# Patent

## Title
Isolated polynucleotides and polypeptides, and methods of using same for increasing nitrogen use efficiency, yield, growth rate, vigor, biomass, oil content, and/or abiotic stress tolerance

## Abstract

Provided are methods of increasing nitrogen use efficiency, yield, biomass, growth rate, vigor, oil content, fiber yield, fiber quality and/or abiotic stress tolerance of a plant by expressing within the plant an exogenous polynucleotide comprising a nucleic acid sequence at least 80% identical to SEQ ID NO:1-467, 785-3047; or an exogenous polynucleotide encoding a polypeptide at least 80% identical to SEQ ID NO:468-784, 3048-4333, 4335-4682. Also provided isolated polynucleotide comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOs:1-467, 785-3047, which can be used to increase nitrogen use efficiency, yield, biomass, growth rate, vigor, oil content, fiber yield, fiber quality and/or abiotic stress tolerance of a plant.

## Background
RELATED APPLICATIONS
This application is a National Phase of PCT Patent Application No. PCT/IB2010/052545 having International filing date of Jun. 8, 2010, which claims the benefit of priority of U.S. Provisional Patent Application Nos. 61/272,764 filed on Oct. 30, 2009, and 61/213,457 filed on Jun. 10, 2009. The contents of the above applications are all incorporated herein by reference.


FIELD AND BACKGROUND OF THE INVENTION
The present invention, in some embodiments thereof, relates to novel polynucleotides and polypeptides which can increase nitrogen use efficiency, fertilizer use efficiency, yield (e.g., seed/grain yield, oil yield), growth rate, vigor, biomass, oil content, fiber yield, fiber quality and/or length, abiotic stress tolerance and/or water use efficiency of a plant.
A common approach to promote plant growth has been, and continues to be, the use of natural as well as synthetic nutrients (fertilizers). Thus, fertilizers are the fuel behind the “green revolution”, directly responsible for the exceptional increase in crop yields during the last 40 years, and are considered the number one overhead expense in agriculture. Of the three macronutrients provided as main fertilizers [Nitrogen (N), Phosphate (P) and Potassium (K)], nitrogen is often the rate-limiting element in plant growth and all field crops have a fundamental dependence on inorganic nitrogenous fertilizer. Nitrogen usually needs to be replenished every year, particularly for cereals, which comprise more than half of the cultivated areas worldwide. For example, inorganic nitrogenous fertilizers such as ammonium nitrate, potassium nitrate, or urea, typically accounts for 40% of the costs associated with crops such as corn and wheat.
Nitrogen is an essential macronutrient for the plant, responsible for biosynthesis of amino and nucleic acids, prosthetic groups, plant hormones, plant chemical defenses, etc. In addition, nitrogen is often the rate-limiting element in plant growth and all field crops have a fundamental dependence on inorganic nitrogen. Thus, nitrogen is translocated to the shoot, where it is stored in the leaves and stalk during the rapid step of plant development and up until flowering. In corn for example, plants accumulate the bulk of their organic nitrogen during the period of grain germination, and until flowering. Once fertilization of the plant has occurred, grains begin to form and become the main sink of plant nitrogen. The stored nitrogen can be then redistributed from the leaves and stalk that served as storage compartments until grain formation.
Since fertilizer is rapidly depleted from most soil types, it must be supplied to growing crops two or three times during the growing season. In addition, the low nitrogen use efficiency (NUE) of the main crops (e.g., in the range of only 30-70%) negatively affects the input expenses for the farmer, due to the excess fertilizer applied. Moreover, the over and inefficient use of fertilizers are major factors responsible for environmental problems such as eutrophication of groundwater, lakes, rivers and seas, nitrate pollution in drinking water which can cause methemoglobinemia, phosphate pollution, atmospheric pollution and the like. However, in spite of the negative impact of fertilizers on the environment, and the limits on fertilizer use, which have been legislated in several countries, the use of fertilizers is expected to increase in order support food and fiber production for rapid population growth on limited land resources. For example, it has been estimated that by 2050, more than 150 million tons of nitrogenous fertilizer will be used worldwide annually.
Increased use efficiency of nitrogen by plants should enable crops to be cultivated with lower fertilizer input, or alternatively to be cultivated on soils of poorer quality and would therefore have significant economic impact in both developed and developing agricultural systems.
Genetic improvement of fertilizer use efficiency (FUE) in plants can be generated either via traditional breeding or via genetic engineering.
Attempts to generate plants with increased FUE have been described in U.S. Pat. Appl. No. 20020046419 to Choo, et al.; U.S. Pat. Appl. No. 2005010879 to Edgerton et al.; U.S. Pat. Appl. No. 20060179511 to Chomet et al.; Good, A, et al. 2007 (Engineering nitrogen use efficiency with alanine aminotransferase. Canadian Journal of Botany 85: 252-262); and Good A G et al. 2004 (Trends Plant Sci. 9:597-605).
Yanagisawa et al. (Proc. Natl. Acad. Sci. U.S.A. 2004 101:7833-8) describe D of 1 transgenic plants which exhibit improved growth under low-nitrogen conditions.
U.S. Pat. No. 6,084,153 to Good et al. discloses the use of a stress responsive promoter to control the expression of Alanine Amine Transferase (AlaAT) and transgenic canola plants with improved drought and nitrogen deficiency tolerance when compared to control plants.
The ever-increasing world population and the decreasing availability in arable land for agriculture affect the yield of plants and plant-related products. The global shortage of water supply, desertification, abiotic stress (ABS) conditions (e.g., salinity, drought, flood, suboptimal temperature and toxic chemical pollution), and/or limited nitrogen and fertilizer sources cause substantial damage to agricultural plants such as major alterations in the plant metabolism, cell death, and decreases in plant growth and crop productivity.
Drought is a gradual phenomenon, which involves periods of abnormally dry weather that persists long enough to produce serious hydrologic imbalances such as crop damage, water supply shortage and increased susceptibility to various diseases.
Salinity, high salt levels, affects one in five hectares of irrigated land. None of the top five food crops, i.e., wheat, corn, rice, potatoes, and soybean, can tolerate excessive salt. Detrimental effects of salt on plants result from both water deficit, which leads to osmotic stress (similar to drought stress), and the effect of excess sodium ions on critical biochemical processes. As with freezing and drought, high salt causes water deficit; and the presence of high salt makes it difficult for plant roots to extract water from their environment. Thus, salination of soils that are used for agricultural production is a significant and increasing problem in regions that rely heavily on agriculture, and is worsen by over-utilization, over-fertilization and water shortage, typically caused by climatic change and the demands of increasing population.
Suboptimal temperatures affect plant growth and development through the whole plant life cycle. Thus, low temperatures reduce germination rate and high temperatures result in leaf necrosis. In addition, mature plants that are exposed to excess of heat may experience heat shock, which may arise in various organs, including leaves and particularly fruit, when transpiration is insufficient to overcome heat stress. Heat also damages cellular structures, including organelles and cytoskeleton, and impairs membrane function. Heat shock may produce a decrease in overall protein synthesis, accompanied by expression of heat shock proteins, e.g., chaperones, which are involved in refolding proteins denatured by heat. High-temperature damage to pollen almost always occurs in conjunction with drought stress, and rarely occurs under well-watered conditions. Combined stress can alter plant metabolism in novel ways. Excessive chilling conditions, e.g., low, but above freezing, temperatures affect crops of tropical origins, such as soybean, rice, maize, and cotton. Typical chilling damage includes wilting, necrosis, chlorosis or leakage of ions from cell membranes. Excessive light conditions, which occur under clear atmospheric conditions subsequent to cold late summer/autumn nights, can lead to photoinhibition of photosynthesis (disruption of photosynthesis). In addition, chilling may lead to yield losses and lower product quality through the delayed ripening of maize.
Nutrient deficiencies cause adaptations of the root architecture, particularly notably for example is the root proliferation within nutrient rich patches to increase nutrient uptake. Nutrient deficiencies cause also the activation of plant metabolic pathways which maximize the absorption, assimilation and distribution processes such as by activating architectural changes. Engineering the expression of the triggered genes may cause the plant to exhibit the architectural changes and enhanced metabolism also under other conditions.
In addition, it is widely known that the plants usually respond to water deficiency by creating a deeper root system that allows access to moisture located in deeper soil layers. Triggering this effect will allow the plants to access nutrients and water located in deeper soil horizons particularly those readily dissolved in water like nitrates.
Yield is affected by various factors, such as, the number and size of the plant organs, plant architecture (for example, the number of branches), grains set length, number of filled grains, vigor (e.g. seedling), growth rate, root development, utilization of water, nutrients (e.g., nitrogen) and fertilizers, and stress tolerance.
Crops such as, corn, rice, wheat, canola and soybean account for over half of total human caloric intake, whether through direct consumption of the seeds themselves or through consumption of meat products raised on processed seeds or forage. Seeds are also a source of sugars, proteins and oils and metabolites used in industrial processes. The ability to increase plant yield, whether through increase dry matter accumulation rate, modifying cellulose or lignin composition, increase stalk strength, enlarge meristem size, change of plant branching pattern, erectness of leaves, increase in fertilization efficiency, enhanced seed dry matter accumulation rate, modification of seed development, enhanced seed filling or by increasing the content of oil, starch or protein in the seeds would have many applications in agricultural and non-agricultural uses such as in the biotechnological production of pharmaceuticals, antibodies or vaccines.
Studies have shown that plant adaptations to adverse environmental conditions are complex genetic traits with polygenic nature. Conventional means for crop and horticultural improvements utilize selective breeding techniques to identify plants having desirable characteristics. However, selective breeding is tedious, time consuming and has an unpredictable outcome. Furthermore, limited germplasm resources for yield improvement and incompatibility in crosses between distantly related plant species represent significant problems encountered in conventional breeding. Advances in genetic engineering have allowed mankind to modify the germplasm of plants by expression of genes-of-interest in plants. Such a technology has the capacity to generate crops or plants with improved economic, agronomic or horticultural traits.
WO publication No. 2009/013750 discloses genes, constructs and methods of increasing abiotic stress tolerance, biomass and/or yield in plants generated thereby.
WO publication No. 2008/122980 discloses genes constructs and methods for increasing oil content, growth rate and biomass of plants.
WO publication No. 2008/075364 discloses polynucleotides involved in plant fiber development and methods of using same.
WO publication No. 2007/049275 discloses isolated polypeptides, polynucleotides encoding same, transgenic plants expressing same and methods of using same for increasing fertilizer use efficiency, plant abiotic stress tolerance and biomass.
WO publication No. 2004/104162 discloses methods of increasing abiotic stress tolerance and/or biomass in plants and plants generated thereby.
WO publication No. 2005/121364 discloses polynucleotides and polypeptides involved in plant fiber development and methods of using same for improving fiber quality, yield and/or biomass of a fiber producing plant.
WO publication No. 2007/020638 discloses methods of increasing abiotic stress tolerance and/or biomass in plants and plants generated thereby.
WO publication No. 2009/083958 discloses methods of increasing water use efficiency, fertilizer use efficiency, biotic/abiotic stress tolerance, yield and biomass in plant and plants generated thereby.
WO publication No. 2010/020941 discloses methods of increasing nitrogen use efficiency, abiotic stress tolerance, yield and biomass in plants and plants generated thereby.
WO publication No. 2009/141824 discloses isolated polynucleotides and methods using same for increasing plant utility.
SUMMARY OF THE INVENTION
According to an aspect of some embodiments of the present invention there is provided a method of increasing nitrogen use efficiency, yield, biomass, growth rate, vigor, oil content, fiber yield, fiber quality, and/or abiotic stress tolerance of a plant, comprising expressing within the plant an exogenous polynucleotide comprising a nucleic acid sequence at least 80% identical to SEQ ID NO:1-467, 785-3046 or 3047, thereby increasing the nitrogen use efficiency, yield, biomass, growth rate, vigor, oil content, fiber yield, fiber quality, and/or abiotic stress tolerance of the plant.
According to an aspect of some embodiments of the present invention there is provided a method of increasing nitrogen use efficiency, yield, biomass, growth rate, vigor, oil content, fiber yield, fiber quality, and/or abiotic stress tolerance of a plant, comprising expressing within the plant an exogenous polynucleotide comprising the nucleic acid sequence selected from the group consisting of SEQ ID NOs:1-467, and 785-3047, thereby increasing the nitrogen use efficiency, yield, biomass, growth rate, vigor, oil content, fiber yield, fiber quality, and/or abiotic stress tolerance of the plant.
According to an aspect of some embodiments of the present invention there is provided a method of increasing nitrogen use efficiency, yield, biomass, growth rate, vigor, oil content, fiber yield, fiber quality, and/or abiotic stress tolerance of a plant, comprising expressing within the plant an exogenous polynucleotide comprising a nucleic acid sequence encoding a polypeptide at least 80% identical to SEQ ID NO:468-784, 3048-4333, 4335-4681 or 4682, thereby increasing the nitrogen use efficiency, yield, biomass, growth rate, vigor, oil content, fiber yield, fiber quality, and/or abiotic stress tolerance of the plant.
According to an aspect of some embodiments of the present invention there is provided a method of increasing nitrogen use efficiency, yield, biomass, growth rate, vigor, oil content, fiber yield, fiber quality, and/or abiotic stress tolerance of a plant, comprising expressing within the plant an exogenous polynucleotide comprising a nucleic acid sequence encoding a polypeptide selected from the group consisting of SEQ ID NOs:468-784, 3048-4333, 4335-4682 and 4334, thereby increasing the nitrogen use efficiency, yield, biomass, growth rate, vigor, oil content, fiber yield, fiber quality, and/or abiotic stress tolerance of the plant.
According to an aspect of some embodiments of the present invention there is provided an isolated polynucleotide comprising a nucleic acid sequence at least 80% identical to SEQ ID NO:1-467, 785-3046 or 3047, wherein the nucleic acid sequence is capable of increasing nitrogen use efficiency, yield, biomass, growth rate, vigor, oil content, fiber yield, fiber quality, and/or abiotic stress tolerance of a plant.
According to an aspect of some embodiments of the present invention there is provided an isolated polynucleotide comprising the nucleic acid sequence selected from the group consisting of SEQ ID NOs:1-467, and 785-3047.
According to an aspect of some embodiments of the present invention there is provided an isolated polynucleotide comprising a nucleic acid sequence encoding a polypeptide which comprises an amino acid sequence at least 80% homologous to the amino acid sequence set forth in SEQ ID NO: 468-784, 3048-4333, 4335-4681 or 4682, wherein the amino acid sequence is capable of increasing nitrogen use efficiency, yield, biomass, growth rate, vigor, oil content, fiber yield, fiber quality, and/or abiotic stress tolerance of the plant.
According to an aspect of some embodiments of the present invention there is provided an isolated polynucleotide comprising a nucleic acid sequence encoding a polypeptide which comprises the amino acid sequence selected from the group consisting of SEQ ID NOs:468-784, 3048-4333, 4335-4682 and 4334.
According to an aspect of some embodiments of the present invention there is provided a nucleic acid construct comprising the isolated polynucleotide of some embodiments of the invention and a promoter for directing transcription of the nucleic acid sequence in a host cell.
According to an aspect of some embodiments of the present invention there is provided an isolated polypeptide comprising an amino acid sequence at least 80% homologous to SEQ ID NO:468-784, 3048-4333, 4335-4681 or 4682, wherein the amino acid sequence is capable of increasing nitrogen use efficiency, yield, biomass, growth rate, vigor, oil content, fiber yield, fiber quality, and/or abiotic stress tolerance of a plant.
According to an aspect of some embodiments of the present invention there is provided an isolated polypeptide comprising the amino acid sequence selected from the group consisting of SEQ ID NOs:468-784, 3048-4333, and 4335-4682.
According to an aspect of some embodiments of the present invention there is provided a plant cell exogenously expressing the polynucleotide of some embodiments of the invention, or the nucleic acid construct of some embodiments of the invention.
According to an aspect of some embodiments of the present invention there is provided a plant cell exogenously expressing the polypeptide of some embodiments of the invention.
According to some embodiments of the invention, the nucleic acid sequence is as set forth in SEQ ID NO:1-467, 785-3046 or 3047.
According to some embodiments of the invention, the polynucleotide consists of the nucleic acid sequence selected from the group consisting of SEQ ID NOs:1-467, and 785-3047.
According to some embodiments of the invention, the nucleic acid sequence encodes an amino acid sequence at least 80% homologous to SEQ ID NO:468-784, 3048-4333, 4335-4681 or 4682.
According to some embodiments of the invention, the nucleic acid sequence encodes the amino acid sequence selected from the group consisting of SEQ ID NOs:468-784, 3048-4333, and 4335-4682.
According to some embodiments of the invention, the plant cell forms part of a plant.
According to some embodiments of the invention, the method further comprising growing the plant expressing the exogenous polynucleotide under the abiotic stress.
According to some embodiments of the invention, the abiotic stress is selected from the group consisting of salinity, drought, water deprivation, flood, etiolation, low temperature, high temperature, heavy metal toxicity, anaerobiosis, nutrient deficiency, nutrient excess, atmospheric pollution and UV irradiation.
According to some embodiments of the invention, the yield comprises seed yield or oil yield.
According to some embodiments of the invention, the promoter is heterologous to the isolated polynucleotide and/or to the host cell.
Unless otherwise defined, all technical and/or scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the invention pertains. Although methods and materials similar or equivalent to those described herein can be used in the practice or testing of embodiments of the invention, exemplary methods and/or materials are described below. In case of conflict, the patent specification, including definitions, will control. In addition, the materials, methods, and examples are illustrative only and are not intended to be necessarily limiting.



BRIEF DESCRIPTION OF THE DRAWINGS
Some embodiments of the invention are herein described, by way of example only, with reference to the accompanying drawings. With specific reference now to the drawings in detail, it is stressed that the particulars shown are by way of example and for purposes of illustrative discussion of embodiments of the invention. In this regard, the description taken with the drawings makes apparent to those skilled in the art how embodiments of the invention may be practiced.
In the drawings:
FIG. 1 is a schematic illustration of the modified pGI binary plasmid containing the new At6669 promoter (SEQ ID NO:4687) and the GUSintron (pQYN—6669) used for expressing the isolated polynucleotide sequences of the invention. RB—T-DNA right border; LB—T-DNA left border; MCS—Multiple cloning site; RE—any restriction enzyme; NOS pro=nopaline synthase promoter; NPT-II=neomycin phosphotransferase gene; NOS ter=nopaline synthase terminator; Poly-A signal (polyadenylation signal); GUSintron—the GUS reporter gene (coding sequence and intron). The isolated polynucleotide sequences of the invention were cloned into the vector while replacing the GUSintron reporter gene.
FIG. 2 is a schematic illustration of the modified pGI binary plasmid containing the new At6669 promoter (SEQ ID NO:4687) (pQFN) used for expressing the isolated polynucleotide sequences of the invention. RB—T-DNA right border; LB—T-DNA left border; MCS—Multiple cloning site; RE—any restriction enzyme; NOS pro=nopaline synthase promoter; NPT-II=neomycin phosphotransferase gene; NOS ter=nopaline synthase terminator; Poly-A signal (polyadenylation signal); GUSintron—the GUS reporter gene (coding sequence and intron). The isolated polynucleotide sequences of the invention were cloned into the MCS of the vector.
FIGS. 3A-F are images depicting visualization of root development of transgenic plants exogenously expressing the polynucleotide of some embodiments of the invention when grown in transparent agar plates under normal (FIGS. 3A-B), osmotic stress (15% PEG; FIGS. 3C-D) or nitrogen-limiting (FIGS. 3E-F) conditions. The different transgenes were grown in transparent agar plates for 17 days (7 days nursery and 10 days after transplanting). The plates were photographed every 3-4 days starting at day 1 after transplanting. FIG. 3A—An image of a photograph of plants taken following 10 after transplanting days on agar plates when grown under normal (standard) conditions. FIG. 3B—An image of root analysis of the plants shown in FIG. 3A in which the lengths of the roots measured are represented by arrows. FIG. 3C—An image of a photograph of plants taken following 10 days after transplanting on agar plates, grown under high osmotic (PEG 15%) conditions. FIG. 3D—An image of root analysis of the plants shown in FIG. 3C in which the lengths of the roots measured are represented by arrows. FIG. 3E—An image of a photograph of plants taken following 10 days after transplanting on agar plates, grown under low nitrogen conditions. FIG. 3F—An image of root analysis of the plants shown in FIG. 3E in which the lengths of the roots measured are represented by arrows.
FIG. 4 is a schematic illustration of the modified pGI binary plasmid containing the Root Promoter (pQNa_RP) used for expressing the isolated polynucleotide sequences of the invention. RB—T-DNA right border; LB—T-DNA left border; NOS pro=nopaline synthase promoter; NPT-II=neomycin phosphotransferase gene; NOS ter=nopaline synthase terminator; Poly-A signal (polyadenylation signal); The isolated polynucleotide sequences according to some embodiments of the invention were cloned into the MCS of the vector.



DESCRIPTION OF SPECIFIC EMBODIMENTS OF THE INVENTION
The present invention, in some embodiments thereof, relates to novel polynucleotides and polypeptides, nucleic acid constructs comprising same, host cells expressing same, transgenic plants exogenously expressing same and, more particularly, but not exclusively, to methods of using same for increasing nitrogen use efficiency, fertilizer use efficiency, yield, growth rate, vigor, biomass, oil content, fiber yield, fiber quality, fiber length, abiotic stress tolerance and/or water use efficiency of a plant.
Before explaining at least one embodiment of the invention in detail, it is to be understood that the invention is not necessarily limited in its application to the details set forth in the following description or exemplified by the Examples. The invention is capable of other embodiments or of being practiced or carried out in various ways.
The present inventors have identified novel polypeptides and polynucleotides which can be used to increase nitrogen use efficiency, fertilizer use efficiency, yield, growth rate, vigor, biomass, oil content, fiber yield, fiber quality, fiber length, abiotic stress tolerance and/or water use efficiency of a plant.
Thus, as shown in the Examples section which follows, the present inventors have utilized bioinformatics tools to identify polynucleotides which enhance nitrogen use efficiency, fertilizer use efficiency, yield (e.g., seed yield, oil yield), growth rate, vigor, biomass, oil content, fiber development (e.g., fiber yield, quality and/or length), abiotic stress tolerance and/or water use efficiency of a plant. Genes which affect the trait-of-interest were identified based on expression profiles of genes of several Arabidopsis, Rice, Sorghum, Barley, Maize and Tomato ecotypes and tissues (Tables 3-84; Examples 3-16), homology with genes known to affect the trait-of-interest and using digital expression profiles in specific tissues and conditions (Table 1, Example 1).
Homologous polypeptides and polynucleotides having the same function were also identified (Table 2, Example 2). Altogether, these results suggest the use of the novel polynucleotides and polypeptides of the invention for increasing nitrogen use efficiency, fertilizer use efficiency, yield (e.g., seed yield, oil yield), growth rate, vigor, biomass, oil content, fiber yield, fiber quality, fiber length, abiotic stress tolerance and/or water use efficiency of a plant.
Thus, according to an aspect of some embodiments of the invention, there is provided method of increasing fertilizer use efficiency, nitrogen use efficiency, yield, biomass, growth rate, vigor, oil content, fiber yield, fiber quality, fiber length, and/or abiotic stress tolerance of a plant, comprising expressing within the plant an exogenous polynucleotide comprising a nucleic acid sequence at least 80% identical to SEQ ID NO:1-467, 785-3046 or 3047, thereby increasing the fertilizer use efficiency, nitrogen use efficiency, yield, biomass, growth rate, vigor, oil content, fiber yield, fiber length, fiber quality, and/or abiotic stress tolerance of the plant.
As used herein the phrase “fertilizer use efficiency” refers to the metabolic process(es) which lead to an increase in the plant's yield, biomass, vigor, and growth rate per fertilizer unit applied. The metabolic process can be the uptake, spread, absorbent, accumulation, relocation (within the plant) and use of one or more of the minerals and organic moieties absorbed by the plant, such as nitrogen, phosphates and/or potassium.
As used herein the phrase “fertilizer-limiting conditions” refers to growth conditions which include a level (e.g., concentration) of a fertilizer applied which is below the level needed for normal plant metabolism, growth, reproduction and/or viability.
As used herein the phrase “nitrogen use efficiency (NUE)” refers to the metabolic process(es) which lead to an increase in the plant's yield, biomass, vigor, and growth rate per nitrogen unit applied. The metabolic process can be the uptake, spread, absorbent, accumulation, relocation (within the plant) and use of nitrogen absorbed by the plant.
As used herein the phrase “nitrogen-limiting conditions” refers to growth conditions which include a level (e.g., concentration) of nitrogen (e.g., ammonium or nitrate) applied which is below the level needed for normal plant metabolism, growth, reproduction and/or viability.
Improved plant NUE and FUE is translated in the field into either harvesting similar quantities of yield, while implementing less fertilizers, or increased yields gained by implementing the same levels of fertilizers. Thus, improved NUE or FUE has a direct effect on plant yield in the field. Thus, the polynucleotides and polypeptides of some embodiments of the invention positively affect plant yield, seed yield, and plant biomass. In addition, the benefit of improved plant NUE will certainly improve crop quality and biochemical constituents of the seed such as protein yield and oil yield.
As used herein the phrase “plant yield” refers to the amount (e.g., as determined by weight or size) or quantity (numbers) of tissues or organs produced per plant or per growing season. Hence increased yield could affect the economic benefit one can obtain from the plant in a certain growing area and/or growing time.
It should be noted that a plant yield can be affected by various parameters including, but not limited to, plant biomass; plant vigor; growth rate; seed yield; seed or grain quantity; seed or grain quality; oil yield; content of oil, starch and/or protein in harvested organs (e.g., seeds or vegetative parts of the plant); number of flowers (florets) per panicle (expressed as a ratio of number of filled seeds over number of primary panicles); harvest index; number of plants grown per area; number and size of harvested organs per plant and per area; number of plants per growing area (density); number of harvested organs in field; total leaf area; carbon assimilation and carbon partitioning (the distribution/allocation of carbon within the plant); resistance to shade; number of harvestable organs (e.g. seeds), seeds per pod, weight per seed; and modified architecture [such as increase stalk diameter, thickness or improvement of physical properties (e.g. elasticity)].
The term “seed” (also referred to as “grain” or “kernel”) as used herein refers to a small embryonic plant enclosed in a covering called the seed coat (usually with some stored food), the product of the ripened ovule of gymnosperm and angiosperm plants which occurs after fertilization and some growth within the mother plant.
As used herein the phrase “seed yield” refers to the number or weight of the seeds per plant, seeds per pod, or per growing area or to the weight of a single seed, or to the oil extracted per seed. Hence seed yield can be affected by seed dimensions (e.g., length, width, perimeter, area and/or volume), number of (filled) seeds and seed filling rate and by seed oil content. Hence increase seed yield per plant could affect the economic benefit one can obtain from the plant in a certain growing area and/or growing time; and increase seed yield per growing area could be achieved by increasing seed yield per plant, and/or by increasing number of plants grown on the same given area.
The phrase “oil content” as used herein refers to the amount of lipids in a given plant organ, either the seeds (seed oil content) or the vegetative portion of the plant (vegetative oil content) and is typically expressed as percentage of dry weight (10% humidity of seeds) or wet weight (for vegetative portion).
It should be noted that oil content is affected by intrinsic oil production of a tissue (e.g., seed, vegetative portion), as well as the mass or size of the oil-producing tissue per plant or per growth period.
In one embodiment, increase in oil content of the plant can be achieved by increasing the size/mass of a plant's tissue(s) which comprise oil per growth period. Thus, increased oil content of a plant can be achieved by increasing the yield, growth rate, biomass and vigor of the plant.
As used herein the phrase “plant biomass” refers to the amount (e.g., measured in grams of air-dry tissue) of a tissue produced from the plant in a growing season, which could also determine or affect the plant yield or the yield per growing area. An increase in plant biomass can be in the whole plant or in parts thereof such as aboveground (harvestable) parts, vegetative biomass, roots and seeds.
As used herein the phrase “growth rate” refers to the increase in plant organ/tissue size per time (can be measured in cm2 per day).
As used herein the phrase “plant vigor” refers to the amount (measured by weight) of tissue produced by the plant in a given time. Hence increased vigor could determine or affect the plant yield or the yield per growing time or growing area. In addition, early vigor (seed and/or seedling) results in improved field stand.
It should be noted that a plant yield can be determined under stress (e.g., abiotic stress, nitrogen-limiting conditions) and/or non-stress (normal) conditions.
As used herein, the phrase “non-stress conditions” refers to the growth conditions (e.g., water, temperature, light-dark cycles, humidity, salt concentration, fertilizer concentration in soil, nutrient supply such as nitrogen, phosphorous and/or potassium), that do not significantly go beyond the everyday climatic and other abiotic conditions that plants may encounter, and which allow optimal growth, metabolism, reproduction and/or viability of a plant at any stage in its life cycle (e.g., in a crop plant from seed to a mature plant and back to seed again). Persons skilled in the art are aware of normal soil conditions and climatic conditions for a given plant in a given geographic location. It should be noted that while the non-stress conditions may include some mild variations from the optimal conditions (which vary from one type/species of a plant to another), such variations do not cause the plant to cease growing without the capacity to resume growth.
The phrase “abiotic stress” as used herein refers to any adverse effect on metabolism, growth, reproduction and/or viability of a plant. Accordingly, abiotic stress can be induced by suboptimal environmental growth conditions such as, for example, salinity, water deprivation, flooding, freezing, low or high temperature, heavy metal toxicity, anaerobiosis, nutrient deficiency, atmospheric pollution or UV irradiation. The implications of abiotic stress are discussed in the Background section.
The phrase “abiotic stress tolerance” as used herein refers to the ability of a plant to endure an abiotic stress without suffering a substantial alteration in metabolism, growth, productivity and/or viability.
As used herein the phrase “water use efficiency (WUE)” refers to the level of organic matter produced per unit of water consumed by the plant, i.e., the dry weight of a plant in relation to the plant's water use, e.g., the biomass produced per unit transpiration.
It should be noted that improved ABST will confer plants with improved vigor also under non-stress conditions, resulting in crops having improved biomass and/or yield e.g., elongated fibers for the cotton industry, higher oil content.
The term “fiber” is usually inclusive of thick-walled conducting cells such as vessels and tracheids and to fibrillar aggregates of many individual fiber cells. Hence, the term “fiber” refers to (a) thick-walled conducting and non-conducting cells of the xylem; (b) fibers of extraxylary origin, including those from phloem, bark, ground tissue, and epidermis; and (c) fibers from stems, leaves, roots, seeds, and flowers or inflorescences (such as those of Sorghum vulgare used in the manufacture of brushes and brooms).
Example of fiber producing plants, include, but are not limited to, agricultural crops such as cotton, silk cotton tree (Kapok, Ceiba pentandra), desert willow, creosote bush, winterfat, balsa, kenaf, roselle, jute, sisal abaca, flax, corn, sugar cane, hemp, ramie, kapok, coir, bamboo, spanish moss and Agave spp. (e.g. sisal).
As used herein the phrase “fiber quality” refers to at least one fiber parameter which is agriculturally desired, or required in the fiber industry (further described hereinbelow). Examples of such parameters, include but are not limited to, fiber length, fiber strength, fiber fitness, fiber weight per unit length, maturity ratio and uniformity (further described hereinbelow.
Cotton fiber (lint) quality is typically measured according to fiber length, strength and fineness. Accordingly, the lint quality is considered higher when the fiber is longer, stronger and finer.
As used herein the phrase “fiber yield” refers to the amount or quantity of fibers produced from the fiber producing plant.
As used herein the term “increasing” refers to at least about 2%, at least about 3%, at least about 4%, at least about 5%, at least about 10%, at least about 15%, at least about 20%, at least about 30%, at least about 40%, at least about 50%, at least about 60%, at least about 70%, at least about 80%, increase in nitrogen use efficiency, fertilizer use efficiency, yield, seed yield, growth rate, vigor, biomass, oil content, fiber yield, fiber quality, fiber length, abiotic stress tolerance and/or water use efficiency of a plant of a plant as compared to a native plant [i.e., a plant not modified with the biomolecules (polynucleotide or polypeptides) of the invention, e.g., a non-transformed plant of the same species which is grown under the same growth conditions).
The phrase “expressing within the plant an exogenous polynucleotide” as used herein refers to upregulating the expression level of an exogenous polynucleotide within the plant by introducing the exogenous polynucleotide into a plant cell or plant and expressing by recombinant means, as further described herein below.
As used herein “expressing” refers to expression at the mRNA and optionally polypeptide level.
As used herein, the phrase “exogenous polynucleotide” refers to a heterologous nucleic acid sequence which may not be naturally expressed within the plant or which overexpression thereof in the plant is desired. The exogenous polynucleotide may be introduced into the plant in a stable or transient manner, so as to produce a ribonucleic acid (RNA) molecule and/or a polypeptide molecule. It should be noted that the exogenous polynucleotide may comprise a nucleic acid sequence which is identical or partially homologous to an endogenous nucleic acid sequence of the plant.
The term “endogenous” as used herein refers to any polynucleotide or polypeptide which is present and/or naturally expressed within a plant or a cell thereof.
According to some embodiments of the invention the exogenous polynucleotide comprises a nucleic acid sequence which is at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99%, e.g., 100% identical to the nucleic acid sequence selected from the group consisting of SEQ ID NOs:1-467, and 785-3047.
Identity (e.g., percent homology) can be determined using any homology comparison software, including for example, the BlastN software of the National Center of Biotechnology Information (NCBI) such as by using default parameters.
According to some embodiments of the invention, the homology is a global homology, i.e., an homology over the entire amino acid or nucleic acid sequences of the invention and not over portions thereof.
According to some embodiments of the invention the exogenous polynucleotide is at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99%, e.g., 100% identical to the polynucleotide selected from the group consisting of SEQ ID NOs:1-467, and 785-3047.
According to some embodiments of the invention the exogenous polynucleotide is set forth by SEQ ID NO:1-467, 785-3046 or 3047.
As used herein the term “polynucleotide” refers to a single or double stranded nucleic acid sequence which is isolated and provided in the form of an RNA sequence, a complementary polynucleotide sequence (cDNA), a genomic polynucleotide sequence and/or a composite polynucleotide sequences (e.g., a combination of the above).
The term “isolated” refers to at least partially separated from the natural environment e.g., from a plant cell.
As used herein the phrase “complementary polynucleotide sequence” refers to a sequence, which results from reverse transcription of messenger RNA using a reverse transcriptase or any other RNA dependent DNA polymerase. Such a sequence can be subsequently amplified in vivo or in vitro using a DNA dependent DNA polymerase.
As used herein the phrase “genomic polynucleotide sequence” refers to a sequence derived (isolated) from a chromosome and thus it represents a contiguous portion of a chromosome.
As used herein the phrase “composite polynucleotide sequence” refers to a sequence, which is at least partially complementary and at least partially genomic. A composite sequence can include some exonal sequences required to encode the polypeptide of the present invention, as well as some intronic sequences interposing therebetween. The intronic sequences can be of any source, including of other genes, and typically will include conserved splicing signal sequences. Such intronic sequences may further include cis acting expression regulatory elements.
According to some embodiments of the invention, the exogenous polynucleotide of the invention encodes a polypeptide having an amino acid sequence at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99%, or more say 100% homologous to the amino acid sequence selected from the group consisting of SEQ ID NOs:468-784, 3048-4333, and 4335-4682.
Homology (e.g., percent homology) can be determined using any homology comparison software, including for example, the BlastP or TBLASTN software of the National Center of Biotechnology Information (NCBI) such as by using default parameters, when starting from a polypeptide sequence; or the tBLASTX algorithm (available via the NCBI) such as by using default parameters, which compares the six-frame conceptual translation products of a nucleotide query sequence (both strands) against a protein sequence database.
Homologous sequences include both orthologous and paralogous sequences. The term “paralogous” relates to gene-duplications within the genome of a species leading to paralogous genes. The term “orthologous” relates to homologous genes in different organisms due to ancestral relationship.
One option to identify orthologues in monocot plant species is by performing a reciprocal blast search. This may be done by a first blast involving blasting the sequence-of-interest against any sequence database, such as the publicly available NCBI database which may be found at: Hypertext Transfer Protocol://World Wide Web (dot) ncbi (dot) nlm (dot) nih (dot) gov. If orthologues in rice were sought, the sequence-of-interest would be blasted against, for example, the 28,469 full-length cDNA clones from Oryza sativa Nipponbare available at NCBI. The blast results may be filtered. The full-length sequences of either the filtered results or the non-filtered results are then blasted back (second blast) against the sequences of the organism from which the sequence-of-interest is derived. The results of the first and second blasts are then compared. An orthologue is identified when the sequence resulting in the highest score (best hit) in the first blast identifies in the second blast the query sequence (the original sequence-of-interest) as the best hit. Using the same rational a paralogue (homolog to a gene in the same organism) is found. In case of large sequence families, the ClustalW program may be used [Hypertext Transfer Protocol://World Wide Web (dot) ebi (dot) ac (dot) uk/Tools/clustalw2/index (dot) html], followed by a neighbor-joining tree (Hypertext Transfer Protocol://en (dot) wikipedia (dot) org/wiki/Neighbor-joining) which helps visualizing the clustering.
According to some embodiments of the invention, the exogenous polynucleotide encodes a polypeptide consisting of the amino acid sequence set forth by SEQ ID NO:468-784, 3048-4333, 4335-4682 or 4334.
Nucleic acid sequences encoding the polypeptides of the present invention may be optimized for expression. Examples of such sequence modifications include, but are not limited to, an altered G/C content to more closely approach that typically found in the plant species of interest, and the removal of codons atypically found in the plant species commonly referred to as codon optimization.
The phrase “codon optimization” refers to the selection of appropriate DNA nucleotides for use within a structural gene or fragment thereof that approaches codon usage within the plant of interest. Therefore, an optimized gene or nucleic acid sequence refers to a gene in which the nucleotide sequence of a native or naturally occurring gene has been modified in order to utilize statistically-preferred or statistically-favored codons within the plant. The nucleotide sequence typically is examined at the DNA level and the coding region optimized for expression in the plant species determined using any suitable procedure, for example as described in Sardana et al. (1996, Plant Cell Reports 15:677-681). In this method, the standard deviation of codon usage, a measure of codon usage bias, may be calculated by first finding the squared proportional deviation of usage of each codon of the native gene relative to that of highly expressed plant genes, followed by a calculation of the average squared deviation. The formula used is: 1 SDCU=n=1 N[(Xn−Yn)/Yn]2/N, where Xn refers to the frequency of usage of codon n in highly expressed plant genes, where Yn to the frequency of usage of codon n in the gene of interest and N refers to the total number of codons in the gene of interest. A Table of codon usage from highly expressed genes of dicotyledonous plants is compiled using the data of Murray et al. (1989, Nuc Acids Res. 17:477-498).
One method of optimizing the nucleic acid sequence in accordance with the preferred codon usage for a particular plant cell type is based on the direct use, without performing any extra statistical calculations, of codon optimization Tables such as those provided on-line at the Codon Usage Database through the NIRS (National Institute of Agrobiological Sciences) DNA bank in Japan (Hypertext Transfer Protocol://World Wide Web (dot) kazusa (dot) or (dot) jp/codon/). The Codon Usage Database contains codon usage tables for a number of different species, with each codon usage Table having been statistically determined based on the data present in Genbank.
By using the above Tables to determine the most preferred or most favored codons for each amino acid in a particular species (for example, rice), a naturally-occurring nucleotide sequence encoding a protein of interest can be codon optimized for that particular plant species. This is effected by replacing codons that may have a low statistical incidence in the particular species genome with corresponding codons, in regard to an amino acid, that are statistically more favored. However, one or more less-favored codons may be selected to delete existing restriction sites, to create new ones at potentially useful junctions (5′ and 3′ ends to add signal peptide or termination cassettes, internal sites that might be used to cut and splice segments together to produce a correct full-length sequence), or to eliminate nucleotide sequences that may negatively effect mRNA stability or expression.
The naturally-occurring encoding nucleotide sequence may already, in advance of any modification, contain a number of codons that correspond to a statistically-favored codon in a particular plant species. Therefore, codon optimization of the native nucleotide sequence may comprise determining which codons, within the native nucleotide sequence, are not statistically-favored with regards to a particular plant, and modifying these codons in accordance with a codon usage table of the particular plant to produce a codon optimized derivative. A modified nucleotide sequence may be fully or partially optimized for plant codon usage provided that the protein encoded by the modified nucleotide sequence is produced at a level higher than the protein encoded by the corresponding naturally occurring or native gene. Construction of synthetic genes by altering the codon usage is described in for example PCT Patent Application 93/07278.
According to some embodiments of the invention, the exogenous polynucleotide is a non-coding RNA.
As used herein the phrase “non-coding RNA” refers to an RNA molecule which does not encode an amino acid sequence (a polypeptide). Examples of such non-coding RNA molecules include, but are not limited to, an antisense RNA, a pre-miRNA (precursor of a microRNA), or a precursor of a Piwi-interacting RNA (piRNA).
Non-limiting examples of non-coding RNA polynucleotides are provided in SEQ ID NOs:214, 215, 216, 466, 467, 967, 968, 969, and 1575.
Thus, the invention encompasses nucleic acid sequences described hereinabove; fragments thereof, sequences hybridizable therewith, sequences homologous thereto, sequences encoding similar polypeptides with different codon usage, altered sequences characterized by mutations, such as deletion, insertion or substitution of one or more nucleotides, either naturally occurring or man induced, either randomly or in a targeted fashion.
The invention provides an isolated polynucleotide comprising a nucleic acid sequence at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99%, e.g., 100% identical to the polynucleotide selected from the group consisting of SEQ ID NOs:1-467, and 785-3047.
According to some embodiments of the invention the nucleic acid sequence is capable of increasing nitrogen use efficiency, fertilizer use efficiency, yield, seed yield, growth rate, vigor, biomass, oil content, fiber yield, fiber quality, fiber length, abiotic stress tolerance and/or water use efficiency of a plant.
According to some embodiments of the invention the isolated polynucleotide comprising the nucleic acid sequence selected from the group consisting of SEQ ID NOs:1-467, and 785-3047.
According to some embodiments of the invention the isolated polynucleotide is set forth by SEQ ID NO:1-467, 785-3046 or 3047.
The invention provides an isolated polynucleotide comprising a nucleic acid sequence encoding a polypeptide which comprises an amino acid sequence at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99%, or more say 100% homologous to the amino acid sequence selected from the group consisting of SEQ ID NO:468-784, 3048-4333, and 4335-4682.
According to some embodiments of the invention the amino acid sequence is capable of increasing nitrogen use efficiency, fertilizer use efficiency, yield, seed yield, growth rate, vigor, biomass, oil content, fiber yield, fiber quality, fiber length, abiotic stress tolerance and/or water use efficiency of a plant.
The invention provides an isolated polynucleotide comprising a nucleic acid sequence encoding a polypeptide which comprises the amino acid sequence selected from the group consisting of SEQ ID NOs:468-784, 3048-4333, 4335-4682 and 4334.
The invention provides an isolated polypeptide comprising an amino acid sequence at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99%, or more say 100% homologous to an amino acid sequence selected from the group consisting of SEQ ID NO: 468-784, 3048-4333, and 4335-4682.
According to some embodiments of the invention, the polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NOs:468-784, 3048-4333, 4335-4682 and 4334.
According to some embodiments of the invention, the polypeptide is set forth by SEQ ID NO: 468-784, 3048-4333, 4335-4682 or 4334.
According to some embodiments of the invention, there is provided a nucleic acid construct comprising the isolated polynucleotide of the invention, and a promoter for directing transcription of the nucleic acid sequence of the isolated polynucleotide in a host cell.
The invention also encompasses fragments of the above described polypeptides and polypeptides having mutations, such as deletions, insertions or substitutions of one or more amino acids, either naturally occurring or man induced, either randomly or in a targeted fashion.
The term “plant” as used herein encompasses whole plants, ancestors and progeny of the plants and plant parts, including seeds, shoots, stems, roots (including tubers), and plant cells, tissues and organs. The plant may be in any form including suspension cultures, embryos, meristematic regions, callus tissue, leaves, gametophytes, sporophytes, pollen, and microspores. Plants that are particularly useful in the methods of the invention include all plants which belong to the superfamily Viridiplantae, in particular monocotyledonous and dicotyledonous plants including a fodder or forage legume, ornamental plant, food crop, tree, or shrub selected from the list comprising Acacia spp., Acer spp., Actinidia spp., Aesculus spp., Agathis australis, Albizia amara, Alsophila tricolor, Andropogon spp., Arachis spp, Areca catechu, Astelia fragrans, Astragalus cicer, Baikiaea plurijuga, Betula spp., Brassica spp., Bruguiera gymnorrhiza, Burkea africana, Butea frondosa, Cadaba farinosa, Calliandra spp, Camellia sinensis, Canna indica, Capsicum spp., Cassia spp., Centroema pubescens, Chacoomeles spp., Cinnamomum cassia, Coffea arabica, Colophospermum mopane, Coronillia varia, Cotoneaster serotina, Crataegus spp., Cucumis spp., Cupressus spp., Cyathea dealbata, Cydonia oblonga, Cryptomeria japonica, Cymbopogon spp., Cynthea dealbata, Cydonia oblonga, Dalbergia monetaria, Davallia divaricata, Desmodium spp., Dicksonia squarosa, Dibeteropogon amplectens, Dioclea spp, Dolichos spp., Dorycnium rectum, Echinochloa pyramidalis, Ehraffia spp., Eleusine coracana, Eragrestis spp., Erythrina spp., Eucalypfus spp., Euclea schimperi, Eulalia vi/losa, Pagopyrum spp., Feijoa sellowlana, Fragaria spp., Flemingia spp, Freycinetia banksli, Geranium thunbergii, GinAgo biloba, Glycine javanica, Gliricidia spp, Gossypium hirsutum, Grevillea spp., Guibourtia coleosperma, Hedysarum spp., Hemaffhia altissima, Heteropogon contoffus, Hordeum vulgare, Hyparrhenia rufa, Hypericum erectum, Hypeffhelia dissolute, Indigo incamata, Iris spp., Leptarrhena pyrolifolia, Lespediza spp., Lettuca spp., Leucaena leucocephala, Loudetia simplex, Lotonus bainesli, Lotus spp., Macrotyloma axillare, Malus spp., Manihot esculenta, Medicago saliva, Metasequoia glyptostroboides, Musa sapientum, Nicotianum spp., Onobrychis spp., Ornithopus spp., Oryza spp., Peltophorum africanum, Pennisetum spp., Persea gratissima, Petunia spp., Phaseolus spp., Phoenix canariensis, Phormium cookianum, Photinia spp., Picea glauca, Pinus spp., Pisum sativam, Podocarpus totara, Pogonarthria fleckii, Pogonaffhria squarrosa, to Populus spp., Prosopis cineraria, Pseudotsuga menziesii, Pterolobium stellatum, Pyrus communis, Quercus spp., Rhaphiolepsis umbellata, Rhopalostylis sapida, Rhus natalensis, Ribes grossularia, Ribes spp., Robinia pseudoacacia, Rosa spp., Rubus spp., Salix spp., Schyzachyrium sanguineum, Sciadopitys vefficillata, Sequoia sempervirens, Sequoiadendron giganteum, Sorghum bicolor, Spinacia spp., Sporobolus fimbriatus, Stiburus alopecuroides, Stylosanthos humilis, Tadehagi spp, Taxodium distichum, Themeda triandra, Trifolium spp., Triticum spp., Tsuga heterophylla, Vaccinium spp., Vicia spp., Vitis vinifera, Watsonia pyramidata, Zantedeschia aethiopica, Zea mays, amaranth, artichoke, asparagus, broccoli, Brussels sprouts, cabbage, canola, carrot, cauliflower, celery, collard greens, flax, kale, lentil, oilseed rape, okra, onion, potato, rice, soybean, straw, sugar beet, sugar cane, sunflower, tomato, squash tea, maize, wheat, barely, rye, oat, peanut, pea, lentil and alfalfa, cotton, rapeseed, canola, pepper, sunflower, tobacco, eggplant, eucalyptus, a tree, an ornamental plant, a perennial grass and a forage crop. Alternatively algae and other non-Viridiplantae can be used for the methods of the present invention.
According to some embodiments of the invention, the plant used by the method of the invention is a crop plant such as rice, maize, wheat, barley, peanut, potato, sesame, olive tree, palm oil, banana, soybean, sunflower, canola, sugarcane, alfalfa, millet, leguminosae (bean, pea), flax, lupinus, rapeseed, tobacco, poplar and cotton.
According to some embodiments of the invention, there is provided a plant cell exogenously expressing the polynucleotide of some embodiments of the invention, the nucleic acid construct of some embodiments of the invention and/or the polypeptide of some embodiments of the invention.
According to some embodiments of the invention, expressing the exogenous polynucleotide of the invention within the plant is effected by transforming one or more cells of the plant with the exogenous polynucleotide, followed by generating a mature plant from the transformed cells and cultivating the mature plant under conditions suitable for expressing the exogenous polynucleotide within the mature plant.
According to some embodiments of the invention, the transformation is effected by introducing to the plant cell a nucleic acid construct which includes the exogenous polynucleotide of some embodiments of the invention and at least one promoter for directing transcription of the exogenous polynucleotide in a host cell (a plant cell). Further details of suitable transformation approaches are provided hereinbelow.
As mentioned, the nucleic acid construct according to some embodiments of the invention comprises a promoter sequence and the isolated polynucleotide of the invention.
According to some embodiments of the invention, the isolated polynucleotide is operably linked to the promoter sequence.
A coding nucleic acid sequence is “operably linked” to a regulatory sequence (e.g., promoter) if the regulatory sequence is capable of exerting a regulatory effect on the coding sequence linked thereto.
As used herein, the term “promoter” refers to a region of DNA which lies upstream of the transcriptional initiation site of a gene to which RNA polymerase binds to initiate transcription of RNA. The promoter controls where (e.g., which portion of a plant) and/or when (e.g., at which stage or condition in the lifetime of an organism) the gene is expressed.
According to some embodiments of the invention the promoter is heterologous to the isolated polynucleotide (e.g., derived from another gene or species with respect to the isolated polynucleotide).
According to some embodiments of the invention the promoter is heterologous to the host cell (e.g., derived from another cell type, or species with respect to the host cell).
According to some embodiments of the invention the promoter is heterologous to the isolated polynucleotide and to the host cell.
Any suitable promoter sequence can be used by the nucleic acid construct of the present invention. Preferably the promoter is a constitutive promoter, a tissue-specific, or an abiotic stress-inducible promoter.
Suitable constitutive promoters include, for example, CaMV 35S promoter (SEQ ID NO:4685; Odell et al., Nature 313:810-812, 1985); Arabidopsis At6669 promoter (SEQ ID NO:4684; see PCT Publication No. WO04081173A2); Arabidopsis new At6669 promoter (SEQ ID NO:4687); maize Ubi 1 (Christensen et al., Plant Sol. Biol. 18:675-689, 1992); rice actin (McElroy et al., Plant Cell 2:163-171, 1990); pEMU (Last et al., Theor. Appl. Genet. 81:581-588, 1991); CaMV 19S (Nilsson et al., Physiol. Plant 100:456-462, 1997); GOS2 (de Pater et al, Plant J Nov; 2(6):837-44, 1992); ubiquitin (Christensen et al, Plant Mol. Biol. 18: 675-689, 1992); Rice cyclophilin (Bucholz et al, Plant Mol. Biol. 25(5):837-43, 1994); Maize H3 histone (Lepetit et al, Mol. Gen. Genet. 231: 276-285, 1992); Actin 2 (An et al, Plant J. 10(1); 107-121, 1996) and Synthetic Super MAS (Ni et al., The Plant Journal 7: 661-76, 1995). Other constitutive promoters include those in U.S. Pat. Nos. 5,659,026, 5,608,149; 5,608,144; 5,604,121; 5,569,597: 5,466,785; 5,399,680; 5,268,463; and 5,608,142.
Suitable tissue-specific promoters include, but not limited to, leaf-specific promoters [such as described, for example, by Yamamoto et al., Plant J. 12:255-265, 1997; Kwon et al., Plant Physiol. 105:357-67, 1994; Yamamoto et al., Plant Cell Physiol. 35:773-778, 1994; Gotor et al., Plant J. 3:509-18, 1993; Orozco et al., Plant Mol. Biol. 23:1129-1138, 1993; and Matsuoka et al., Proc. Natl. Acad. Sci. USA 90:9586-9590, 1993], seed-preferred promoters [e.g., Napin (originated from Brassica napus which is characterized by a seed specific promoter activity; Stuitje A. R. et. al. Plant Biotechnology Journal 1 (4): 301-309; SEQ ID NO:4686), from seed specific genes (Simon, et al., Plant Mol. Biol. 5. 191, 1985; Scofield, et al., J. Biol. Chem. 262: 12202, 1987; Baszczynski, et al., Plant Mol. Biol. 14: 633, 1990), Brazil Nut albumin (Pearson′ et al., Plant Mol. Biol. 18: 235-245, 1992), legumin (Ellis, et al. Plant Mol. Biol. 10: 203-214, 1988), Glutelin (rice) (Takaiwa, et al., Mol. Gen. Genet. 208: 15-22, 1986; Takaiwa, et al., FEBS Letts. 221: 43-47, 1987), Zein (Matzke et al Plant Mol Biol, 143). 323-32 1990), napA (Stalberg, et al, Planta 199: 515-519, 1996), Wheat SPA (Albani et al, Plant Cell, 9: 171-184, 1997), sunflower oleosin (Cummins, et al., Plant Mol. Biol. 19: 873-876, 1992)], endosperm specific promoters [e.g., wheat LMW and HMW, glutenin-1 (Mol Gen Genet. 216:81-90, 1989; NAR 17:461-2), wheat a, b and g gliadins (EMB03:1409-15, 1984), Barley ltrl promoter, barley B1, C, D hordein (Theor Appl Gen 98:1253-62, 1999; Plant J 4:343-55, 1993; Mol Gen Genet. 250:750-60, 1996), Barley DOF (Mena et al, The Plant Journal, 116(1): 53-62, 1998), Biz2 (EP99106056.7), Synthetic promoter (Vicente-Carbajosa et al., Plant J. 13: 629-640, 1998), rice prolamin NRP33, rice-globulin Glb-1 (Wu et al, Plant Cell Physiology 39(8) 885-889, 1998), rice alpha-globulin REB/OHP-1 (Nakase et al. Plant Mol. Biol. 33: 513-S22, 1997), rice ADP-glucose PP (Trans Res 6:157-68, 1997), maize ESR gene family (Plant J 12:235-46, 1997), sorgum gamma-kafirin (PMB 32:1029-35, 1996)], embryo specific promoters [e.g., rice OSH1 (Sato et al, Proc. Natl. Acad. Sci. USA, 93: 8117-8122), KNOX (Postma-Haarsma et al, Plant Mol. Biol. 39:257-71, 1999), rice oleosin (Wu et at, J. Biochem., 123:386, 1998)], and flower-specific promoters [e.g., AtPRP4, chalene synthase (chsA) (Van der Meer, et al., Plant Mol. Biol. 15, 95-109, 1990), LAT52 (Twell et al Mol. Gen. Genet. 217:240-245; 1989), apetala-3], and root promoters such as the RootP promoter [SEQ ID NO:4688; Upstream region of the gene ATXTH19 (AT4G30290, Xyloglucan endotransglucosylase/hydrolase 19, described in Vissenberg K, et al. Plant Cell Physiol. 2005 January; 46(1):192-200].
Suitable abiotic stress-inducible promoters include, but not limited to, salt-inducible promoters such as RD29A (Yamaguchi-Shinozalei et al., Mol. Gen. Genet. 236:331-340, 1993); drought-inducible promoters such as maize rab17 gene promoter (Pla et. al., Plant Mol. Biol. 21:259-266, 1993), maize rab28 gene promoter (Busk et. al., Plant J. 11:1285-1295, 1997) and maize Ivr2 gene promoter (Pelleschi et. al., Plant Mol. Biol. 39:373-380, 1999); heat-inducible promoters such as heat tomato hsp80-promoter from tomato (U.S. Pat. No. 5,187,267).
The nucleic acid construct of some embodiments of the invention can further include an appropriate selectable marker and/or an origin of replication. According to some embodiments of the invention, the nucleic acid construct utilized is a shuttle vector, which can propagate both in E. coli (wherein the construct comprises an appropriate selectable marker and origin of replication) and be compatible with propagation in cells. The construct according to the present invention can be, for example, a plasmid, a bacmid, a phagemid, a cosmid, a phage, a virus or an artificial chromosome.
The nucleic acid construct of some embodiments of the invention can be utilized to stably or transiently transform plant cells. In stable transformation, the exogenous polynucleotide is integrated into the plant genome and as such it represents a stable and inherited trait. In transient transformation, the exogenous polynucleotide is expressed by the cell transformed but it is not integrated into the genome and as such it represents a transient trait.
There are various methods of introducing foreign genes into both monocotyledonous and dicotyledonous plants (Potrykus, I., Annu. Rev. Plant. Physiol., Plant. Mol. Biol. (1991) 42:205-225; Shimamoto et al., Nature (1989) 338:274-276). The principle methods of causing stable integration of exogenous DNA into plant genomic DNA include two main approaches:
(i) Agrobacterium-mediated gene transfer: Klee et al. (1987) Annu. Rev. Plant Physiol. 38:467-486; Klee and Rogers in Cell Culture and Somatic Cell Genetics of Plants, Vol. 6, Molecular Biology of Plant Nuclear Genes, eds. Schell, J., and Vasil, L. K., Academic Publishers, San Diego, Calif. (1989) p. 2-25; Gatenby, in Plant Biotechnology, eds. Kung, S, and Arntzen, C. J., Butterworth Publishers, Boston, Mass. (1989) p. 93-112.
(ii) Direct DNA uptake: Paszkowski et al., in Cell Culture and Somatic Cell Genetics of Plants, Vol. 6, Molecular Biology of Plant Nuclear Genes eds. Schell, J., and Vasil, L. K., Academic Publishers, San Diego, Calif. (1989) p. 52-68; including methods for direct uptake of DNA into protoplasts, Toriyama, K. et al. (1988) Bio/Technology 6:1072-1074. DNA uptake induced by brief electric shock of plant cells: Zhang et al. Plant Cell Rep. (1988) 7:379-384. Fromm et al. Nature (1986) 319:791-793. DNA injection into plant cells or tissues by particle bombardment, Klein et al. Bio/Technology (1988) 6:559-563; McCabe et al. Bio/Technology (1988) 6:923-926; Sanford, Physiol. Plant. (1990) 79:206-209; by the use of micropipette systems: Neuhaus et al., Theor. Appl. Genet. (1987) 75:30-36; Neuhaus and Spangenberg, Physiol. Plant. (1990) 79:213-217; glass fibers or silicon carbide whisker transformation of cell cultures, embryos or callus tissue, U.S. Pat. No. 5,464,765 or by the direct incubation of DNA with germinating pollen, DeWet et al. in Experimental Manipulation of Ovule Tissue, eds. Chapman, G. P. and Mantell, S. H. and Daniels, W. Longman, London, (1985) p. 197-209; and Ohta, Proc. Natl. Acad. Sci. USA (1986) 83:715-719.
The Agrobacterium system includes the use of plasmid vectors that contain defined DNA segments that integrate into the plant genomic DNA. Methods of inoculation of the plant tissue vary depending upon the plant species and the Agrobacterium delivery system. A widely used approach is the leaf disc procedure which can be performed with any tissue explant that provides a good source for initiation of whole plant differentiation. See, e.g., Horsch et al. in Plant Molecular Biology Manual A5, Kluwer Academic Publishers, Dordrecht (1988) p. 1-9. A supplementary approach employs the Agrobacterium delivery system in combination with vacuum infiltration. The Agrobacterium system is especially viable in the creation of transgenic dicotyledonous plants.
There are various methods of direct DNA transfer into plant cells. In electroporation, the protoplasts are briefly exposed to a strong electric field. In microinjection, the DNA is mechanically injected directly into the cells using very small micropipettes. In microparticle bombardment, the DNA is adsorbed on microprojectiles such as magnesium sulfate crystals or tungsten particles, and the microprojectiles are physically accelerated into cells or plant tissues.
Following stable transformation plant propagation is exercised. The most common method of plant propagation is by seed. Regeneration by seed propagation, however, has the deficiency that due to heterozygosity there is a lack of uniformity in the crop, since seeds are produced by plants according to the genetic variances governed by Mendelian rules. Basically, each seed is genetically different and each will grow with its own specific traits. Therefore, it is preferred that the transformed plant be produced such that the regenerated plant has the identical traits and characteristics of the parent transgenic plant. Therefore, it is preferred that the transformed plant be regenerated by micropropagation which provides a rapid, consistent reproduction of the transformed plants.
Micropropagation is a process of growing new generation plants from a single piece of tissue that has been excised from a selected parent plant or cultivar. This process permits the mass reproduction of plants having the preferred tissue expressing the fusion protein. The new generation plants which are produced are genetically identical to, and have all of the characteristics of, the original plant. Micropropagation allows mass production of quality plant material in a short period of time and offers a rapid multiplication of selected cultivars in the preservation of the characteristics of the original transgenic or transformed plant. The advantages of cloning plants are the speed of plant multiplication and the quality and uniformity of plants produced.
Micropropagation is a multi-stage procedure that requires alteration of culture medium or growth conditions between stages. Thus, the micropropagation process involves four basic stages: Stage one, initial tissue culturing; stage two, tissue culture multiplication; stage three, differentiation and plant formation; and stage four, greenhouse culturing and hardening. During stage one, initial tissue culturing, the tissue culture is established and certified contaminant-free. During stage two, the initial tissue culture is multiplied until a sufficient number of tissue samples are produced to meet production goals. During stage three, the tissue samples grown in stage two are divided and grown into individual plantlets. At stage four, the transformed plantlets are transferred to a greenhouse for hardening where the plants' tolerance to light is gradually increased so that it can be grown in the natural environment.
According to some embodiments of the invention, the transgenic plants are generated by transient transformation of leaf cells, meristematic cells or the whole plant.
Transient transformation can be effected by any of the direct DNA transfer methods described above or by viral infection using modified plant viruses.
Viruses that have been shown to be useful for the transformation of plant hosts include CaMV, Tobacco mosaic virus (TMV), brome mosaic virus (BMV) and Bean Common Mosaic Virus (BV or BCMV). Transformation of plants using plant viruses is described in U.S. Pat. No. 4,855,237 (bean golden mosaic virus; BGV), EP-A 67,553 (TMV), Japanese Published Application No. 63-14693 (TMV), EPA 194,809 (BV), EPA 278,667 (BV); and Gluzman, Y. et al., Communications in Molecular Biology: Viral Vectors, Cold Spring Harbor Laboratory, New York, pp. 172-189 (1988). Pseudovirus particles for use in expressing foreign DNA in many hosts, including plants are described in WO 87/06261.
According to some embodiments of the invention, the virus used for transient transformations is avirulent and thus is incapable of causing severe symptoms such as reduced growth rate, mosaic, ring spots, leaf roll, yellowing, streaking, pox formation, tumor formation and pitting. A suitable avirulent virus may be a naturally occurring avirulent virus or an artificially attenuated virus. Virus attenuation may be effected by using methods well known in the art including, but not limited to, sub-lethal heating, chemical treatment or by directed mutagenesis techniques such as described, for example, by Kurihara and Watanabe (Molecular Plant Pathology 4:259-269, 2003), Gal-on et al. (1992), Atreya et al. (1992) and Huet et al. (1994).
Suitable virus strains can be obtained from available sources such as, for example, the American Type culture Collection (ATCC) or by isolation from infected plants. Isolation of viruses from infected plant tissues can be effected by techniques well known in the art such as described, for example by Foster and Tatlor, Eds. “Plant Virology Protocols: From Virus Isolation to Transgenic Resistance (Methods in Molecular Biology (Humana Pr), Vol 81)”, Humana Press, 1998. Briefly, tissues of an infected plant believed to contain a high concentration of a suitable virus, preferably young leaves and flower petals, are ground in a buffer solution (e.g., phosphate buffer solution) to produce a virus infected sap which can be used in subsequent inoculations.
Construction of plant RNA viruses for the introduction and expression of non-viral exogenous polynucleotide sequences in plants is demonstrated by the above references as well as by Dawson, W. O. et al., Virology (1989) 172:285-292; Takamatsu et al. EMBO J. (1987) 6:307-311; French et al. Science (1986) 231:1294-1297; Takamatsu et al. FEBS Letters (1990) 269:73-76; and U.S. Pat. No. 5,316,931.
When the virus is a DNA virus, suitable modifications can be made to the virus itself. Alternatively, the virus can first be cloned into a bacterial plasmid for ease of constructing the desired viral vector with the foreign DNA. The virus can then be excised from the plasmid. If the virus is a DNA virus, a bacterial origin of replication can be attached to the viral DNA, which is then replicated by the bacteria. Transcription and translation of this DNA will produce the coat protein which will encapsidate the viral DNA. If the virus is an RNA virus, the virus is generally cloned as a cDNA and inserted into a plasmid. The plasmid is then used to make all of the constructions. The RNA virus is then produced by transcribing the viral sequence of the plasmid and translation of the viral genes to produce the coat protein(s) which encapsidate the viral RNA.
In one embodiment, a plant viral polynucleotide is provided in which the native coat protein coding sequence has been deleted from a viral polynucleotide, a non-native plant viral coat protein coding sequence and a non-native promoter, preferably the subgenomic promoter of the non-native coat protein coding sequence, capable of expression in the plant host, packaging of the recombinant plant viral polynucleotide, and ensuring a systemic infection of the host by the recombinant plant viral polynucleotide, has been inserted. Alternatively, the coat protein gene may be inactivated by insertion of the non-native polynucleotide sequence within it, such that a protein is produced. The recombinant plant viral polynucleotide may contain one or more additional non-native subgenomic promoters. Each non-native subgenomic promoter is capable of transcribing or expressing adjacent genes or polynucleotide sequences in the plant host and incapable of recombination with each other and with native subgenomic promoters. Non-native (foreign) polynucleotide sequences may be inserted adjacent the native plant viral subgenomic promoter or the native and a non-native plant viral subgenomic promoters if more than one polynucleotide sequence is included. The non-native polynucleotide sequences are transcribed or expressed in the host plant under control of the subgenomic promoter to produce the desired products.
In a second embodiment, a recombinant plant viral polynucleotide is provided as in the first embodiment except that the native coat protein coding sequence is placed adjacent one of the non-native coat protein subgenomic promoters instead of a non-native coat protein coding sequence.
In a third embodiment, a recombinant plant viral polynucleotide is provided in which the native coat protein gene is adjacent its subgenomic promoter and one or more non-native subgenomic promoters have been inserted into the viral polynucleotide. The inserted non-native subgenomic promoters are capable of transcribing or expressing adjacent genes in a plant host and are incapable of recombination with each other and with native subgenomic promoters. Non-native polynucleotide sequences may be inserted adjacent the non-native subgenomic plant viral promoters such that the sequences are transcribed or expressed in the host plant under control of the subgenomic promoters to produce the desired product.
In a fourth embodiment, a recombinant plant viral polynucleotide is provided as in the third embodiment except that the native coat protein coding sequence is replaced by a non-native coat protein coding sequence.
The viral vectors are encapsidated by the coat proteins encoded by the recombinant plant viral polynucleotide to produce a recombinant plant virus. The recombinant plant viral polynucleotide or recombinant plant virus is used to infect appropriate host plants. The recombinant plant viral polynucleotide is capable of replication in the host, systemic spread in the host, and transcription or expression of foreign gene(s) (exogenous polynucleotide) in the host to produce the desired protein.
Techniques for inoculation of viruses to plants may be found in Foster and Taylor, eds. “Plant Virology Protocols: From Virus Isolation to Transgenic Resistance (Methods in Molecular Biology (Humana Pr), Vol 81)”, Humana Press, 1998; Maramorosh and Koprowski, eds. “Methods in Virology” 7 vols, Academic Press, New York 1967-1984; Hill, S. A. “Methods in Plant Virology”, Blackwell, Oxford, 1984; Walkey, D. G. A. “Applied Plant Virology”, Wiley, New York, 1985; and Kado and Agrawa, eds. “Principles and Techniques in Plant Virology”, Van Nostrand-Reinhold, New York.
In addition to the above, the polynucleotide of the present invention can also be introduced into a chloroplast genome thereby enabling chloroplast expression.
A technique for introducing exogenous polynucleotide sequences to the genome of the chloroplasts is known. This technique involves the following procedures. First, plant cells are chemically treated so as to reduce the number of chloroplasts per cell to about one. Then, the exogenous polynucleotide is introduced via particle bombardment into the cells with the aim of introducing at least one exogenous polynucleotide molecule into the chloroplasts. The exogenous polynucleotides selected such that it is integratable into the chloroplast's genome via homologous recombination which is readily effected by enzymes inherent to the chloroplast. To this end, the exogenous polynucleotide includes, in addition to a gene of interest, at least one polynucleotide stretch which is derived from the chloroplast's genome. In addition, the exogenous polynucleotide includes a selectable marker, which serves by sequential selection procedures to ascertain that all or substantially all of the copies of the chloroplast genomes following such selection will include the exogenous polynucleotide. Further details relating to this technique are found in U.S. Pat. Nos. 4,945,050; and 5,693,507 which are incorporated herein by reference. A polypeptide can thus be produced by the protein expression system of the chloroplast and become integrated into the chloroplast's inner membrane.
Since processes which increase nitrogen use efficiency, fertilizer use efficiency, oil content, yield, seed yield, fiber yield, fiber quality, fiber length, growth rate, biomass, vigor and/or abiotic stress tolerance of a plant can involve multiple genes acting additively or in synergy (see, for example, in Quesda et al., Plant Physiol. 130:951-063, 2002), the present invention also envisages expressing a plurality of exogenous polynucleotides in a single host plant to thereby achieve superior effect on oil content, yield, growth rate, biomass, vigor and/or abiotic stress tolerance.
Expressing a plurality of exogenous polynucleotides in a single host plant can be effected by co-introducing multiple nucleic acid constructs, each including a different exogenous polynucleotide, into a single plant cell. The transformed cell can than be regenerated into a mature plant using the methods described hereinabove.
Alternatively, expressing a plurality of exogenous polynucleotides in a single host plant can be effected by co-introducing into a single plant-cell a single nucleic-acid construct including a plurality of different exogenous polynucleotides. Such a construct can be designed with a single promoter sequence which can transcribe a polycistronic messenger RNA including all the different exogenous polynucleotide sequences. To enable co-translation of the different polypeptides encoded by the polycistronic messenger RNA, the polynucleotide sequences can be inter-linked via an internal ribosome entry site (IRES) sequence which facilitates translation of polynucleotide sequences positioned downstream of the IRES sequence. In this case, a transcribed polycistronic RNA molecule encoding the different polypeptides described above will be translated from both the capped 5′ end and the two internal IRES sequences of the polycistronic RNA molecule to thereby produce in the cell all different polypeptides. Alternatively, the construct can include several promoter sequences each linked to a different exogenous polynucleotide sequence.
The plant cell transformed with the construct including a plurality of different exogenous polynucleotides, can be regenerated into a mature plant, using the methods described hereinabove.
Alternatively, expressing a plurality of exogenous polynucleotides in a single host plant can be effected by introducing different nucleic acid constructs, including different exogenous polynucleotides, into a plurality of plants. The regenerated transformed plants can then be cross-bred and resultant progeny selected for superior abiotic stress tolerance, water use efficiency, fertilizer use efficiency, growth, biomass, yield and/or vigor traits, using conventional plant breeding techniques.
According to some embodiments of the invention, the method further comprising growing the plant expressing the exogenous polynucleotide under the abiotic stress.
Non-limiting examples of abiotic stress conditions include, salinity, drought, water deprivation, excess of water (e.g., flood, waterlogging), etiolation, low temperature, high temperature, heavy metal toxicity, anaerobiosis, nutrient deficiency, nutrient excess, atmospheric pollution and UV irradiation.
Thus, the invention encompasses plants exogenously expressing the polynucleotide(s), the nucleic acid constructs and/or polypeptide(s) of the invention. Once expressed within the plant cell or the entire plant, the level of the polypeptide encoded by the exogenous polynucleotide can be determined by methods well known in the art such as, activity assays, Western blots using antibodies capable of specifically binding the polypeptide, Enzyme-Linked Immuno Sorbent Assay (ELISA), radio-immuno-assays (RIA), immunohistochemistry, immunocytochemistry, immunofluorescence and the like.
Methods of determining the level in the plant of the RNA transcribed from the exogenous polynucleotide are well known in the art and include, for example, Northern blot analysis, reverse transcription polymerase chain reaction (RT-PCR) analysis (including quantitative, semi-quantitative or real-time RT-PCR) and RNA-in situ hybridization.
The sequence information and annotations uncovered by the present teachings can be harnessed in favor of classical breeding. Thus, sub-sequence data of those polynucleotides described above, can be used as markers for marker assisted selection (MAS), in which a marker is used for indirect selection of a genetic determinant or determinants of a trait of interest (e.g., biomass, growth rate, oil content, yield, abiotic stress tolerance, water use efficiency, nitrogen use efficiency and/or fertilizer use efficiency). Nucleic acid data of the present teachings (DNA or RNA sequence) may contain or be linked to polymorphic sites or genetic markers on the genome such as restriction fragment length polymorphism (RFLP), microsatellites and single nucleotide polymorphism (SNP), DNA fingerprinting (DFP), amplified fragment length polymorphism (AFLP), expression level polymorphism, polymorphism of the encoded polypeptide and any other polymorphism at the DNA or RNA sequence.
Examples of marker assisted selections include, but are not limited to, selection for a morphological trait (e.g., a gene that affects form, coloration, male sterility or resistance such as the presence or absence of awn, leaf sheath coloration, height, grain color, aroma of rice); selection for a biochemical trait (e.g., a gene that encodes a protein that can be extracted and observed; for example, isozymes and storage proteins); selection for a biological trait (e.g., pathogen races or insect biotypes based on host pathogen or host parasite interaction can be used as a marker since the genetic constitution of an organism can affect its susceptibility to pathogens or parasites).
The polynucleotides and polypeptides described hereinabove can be used in a wide range of economical plants, in a safe and cost effective manner.
Plant lines exogenously expressing the polynucleotide or the polypeptide of the invention are screened to identify those that show the greatest increase of the desired plant trait.
The effect of the transgene (the exogenous polynucleotide encoding the polypeptide) on abiotic stress tolerance can be determined using known methods such as detailed below and in the Examples section which follows.
Abiotic stress tolerance—Transformed (i.e., expressing the transgene) and non-transformed (wild type) plants are exposed to an abiotic stress condition, such as water deprivation, suboptimal temperature (low temperature, high temperature), nutrient deficiency, nutrient excess, a salt stress condition, osmotic stress, heavy metal toxicity, anaerobiosis, atmospheric pollution and UV irradiation.
Salinity tolerance assay—Transgenic plants with tolerance to high salt concentrations are expected to exhibit better germination, seedling vigor or growth in high salt. Salt stress can be effected in many ways such as, for example, by irrigating the plants with a hyperosmotic solution, by cultivating the plants hydroponically in a hyperosmotic growth solution (e.g., Hoagland solution), or by culturing the plants in a hyperosmotic growth medium [e.g., 50% Murashige-Skoog medium (MS medium)]. Since different plants vary considerably in their tolerance to salinity, the salt concentration in the irrigation water, growth solution, or growth medium can be adjusted according to the specific characteristics of the specific plant cultivar or variety, so as to inflict a mild or moderate effect on the physiology and/or morphology of the plants (for guidelines as to appropriate concentration see, Bernstein and Kafkafi, Root Growth Under Salinity Stress In: Plant Roots, The Hidden Half 3rd ed. Waisel Y, Eshel A and Kafkafi U. (editors) Marcel Dekker Inc., New York, 2002, and reference therein).
For example, a salinity tolerance test can be performed by irrigating plants at different developmental stages with increasing concentrations of sodium chloride (for example 50 mM, 100 mM, 200 mM, 400 mM NaCl) applied from the bottom and from above to ensure even dispersal of salt. Following exposure to the stress condition the plants are frequently monitored until substantial physiological and/or morphological effects appear in wild type plants. Thus, the external phenotypic appearance, degree of wilting and overall success to reach maturity and yield progeny are compared between control and transgenic plants.
Quantitative parameters of tolerance measured include, but are not limited to, the average wet and dry weight, growth rate, leaf size, leaf coverage (overall leaf area), the weight of the seeds yielded, the average seed size and the number of seeds produced per plant. Transformed plants not exhibiting substantial physiological and/or morphological effects, or exhibiting higher biomass than wild-type plants, are identified as abiotic stress tolerant plants.
Osmotic tolerance test—Osmotic stress assays (including sodium chloride and mannitol assays) are conducted to determine if an osmotic stress phenotype was sodium chloride-specific or if it was a general osmotic stress related phenotype. Plants which are tolerant to osmotic stress may have more tolerance to drought and/or freezing. For salt and osmotic stress germination experiments, the medium is supplemented for example with 50 mM, 100 mM, 200 mM NaCl or 100 mM, 200 mM NaCl, 400 mM mannitol.
Drought tolerance assay/Osmoticum assay—Tolerance to drought is performed to identify the genes conferring better plant survival after acute water deprivation. To analyze whether the transgenic plants are more tolerant to drought, an osmotic stress produced by the non-ionic osmolyte sorbitol in the medium can be performed. Control and transgenic plants are germinated and grown in plant-agar plates for 4 days, after which they are transferred to plates containing 500 mM sorbitol. The treatment causes growth retardation, then both control and transgenic plants are compared, by measuring plant weight (wet and dry), yield, and by growth rates measured as time to flowering.
Conversely, soil-based drought screens are performed with plants overexpressing the polynucleotides detailed above. Seeds from control Arabidopsis plants, or other transgenic plants overexpressing the polypeptide of the invention are germinated and transferred to pots. Drought stress is obtained after irrigation is ceased accompanied by placing the pots on absorbent paper to enhance the soil-drying rate. Transgenic and control plants are compared to each other when the majority of the control plants develop severe wilting. Plants are re-watered after obtaining a significant fraction of the control plants displaying a severe wilting. Plants are ranked comparing to controls for each of two criteria: tolerance to the drought conditions and recovery (survival) following re-watering.
Cold stress tolerance—To analyze cold stress, mature (25 day old) plants are transferred to 4° C. chambers for 1 or 2 weeks, with constitutive light. Later on plants are moved back to greenhouse. Two weeks later damages from chilling period, resulting in growth retardation and other phenotypes, are compared between both control and transgenic plants, by measuring plant weight (wet and dry), and by comparing growth rates measured as time to flowering, plant size, yield, and the like.
Heat stress tolerance—Heat stress tolerance is achieved by exposing the plants to temperatures above 34° C. for a certain period. Plant tolerance is examined after transferring the plants back to 22° C. for recovery and evaluation after 5 days relative to internal controls (non-transgenic plants) or plants not exposed to neither cold or heat stress.
Water use efficiency—can be determined as the biomass produced per unit transpiration. To analyze WUE, leaf relative water content can be measured in control and transgenic plants. Fresh weight (FW) is immediately recorded; then leaves are soaked for 8 hours in distilled water at room temperature in the dark, and the turgid weight (TW) is recorded. Total dry weight (DW) is recorded after drying the leaves at 60° C. to a constant weight. Relative water content (RWC) is calculated according to the following Formula I:

RWC=[(FW−DW)/(TW−DW)]×100  Formula I

Fertilizer use efficiency—To analyze whether the transgenic plants are more responsive to fertilizers, plants are grown in agar plates or pots with a limited amount of fertilizer, as described, for example, in Examples 14, 15 and 16, hereinbelow and in Yanagisawa et al (Proc Natl Acad Sci USA. 2004; 101:7833-8). The plants are analyzed for their overall size, time to flowering, yield, protein content of shoot and/or grain. The parameters checked are the overall size of the mature plant, its wet and dry weight, the weight of the seeds yielded, the average seed size and the number of seeds produced per plant. Other parameters that may be tested are: the chlorophyll content of leaves (as nitrogen plant status and the degree of leaf verdure is highly correlated), amino acid and the total protein content of the seeds or other plant parts such as leaves or shoots, oil content, etc. Similarly, instead of providing nitrogen at limiting amounts, phosphate or potassium can be added at increasing concentrations. Again, the same parameters measured are the same as listed above. In this way, nitrogen use efficiency (NUE), phosphate use efficiency (PUE) and potassium use efficiency (KUE) are assessed, checking the ability of the transgenic plants to thrive under nutrient restraining conditions.

Nitrogen use efficiency—To analyze whether the transgenic plants (e.g., Arabidopsis plants) are more responsive to nitrogen, plant are grown in 0.75-3 mM (nitrogen deficient conditions) or 6-10 mM (optimal nitrogen concentration). Plants are allowed to grow for additional 25 days or until seed production. The plants are then analyzed for their overall size, time to flowering, yield, protein content of shoot and/or grain/seed production. The parameters checked can be the overall size of the plant, wet and dry weight, the weight of the seeds yielded, the average seed size and the number of seeds produced per plant. Other parameters that may be tested are: the chlorophyll content of leaves (as nitrogen plant status and the degree of leaf greenness is highly correlated), amino acid and the total protein content of the seeds or other plant parts such as leaves or shoots and oil content. Transformed plants not exhibiting substantial physiological and/or morphological effects, or exhibiting higher measured parameters levels than wild-type plants, are identified as nitrogen use efficient plants.
Nitrogen use efficiency assay using plantlets—The assay is done according to Yanagisawa-S. et al. with minor modifications (“Metabolic engineering with Dof1 transcription factor in plants: Improved nitrogen assimilation and growth under low-nitrogen conditions” Proc. Natl. Acad. Sci. USA 101, 7833-7838). Briefly, transgenic plants which are grown for 7-10 days in 0.5×MS [Murashige-Skoog] supplemented with a selection agent are transferred to two nitrogen-limiting conditions: MS media in which the combined nitrogen concentration (NH4NO3 and KNO3) was 0.75 mM (nitrogen deficient conditions) or 6-15 mM (optimal nitrogen concentration). Plants are allowed to grow for additional 30-40 days and then photographed, individually removed from the Agar (the shoot without the roots) and immediately weighed (fresh weight) for later statistical analysis. Constructs for which only T1 seeds are available are sown on selective media and at least 20 seedlings (each one representing an independent transformation event) are carefully transferred to the nitrogen-limiting media. For constructs for which T2 seeds are available, different transformation events are analyzed. Usually, 20 randomly selected plants from each event are transferred to the nitrogen-limiting media allowed to grow for 3-4 additional weeks and individually weighed at the end of that period. Transgenic plants are compared to control plants grown in parallel under the same conditions. Mock-transgenic plants expressing the uidA reporter gene (GUS) under the same promoter or transgenic plants carrying the same promoter but lacking a reporter gene are used as control.
Nitrogen determination—The procedure for N (nitrogen) concentration determination in the structural parts of the plants involves the potassium persulfate digestion method to convert organic N to NO3− (Purcell and King 1996 Argon. J. 88:111-113, the modified Cd− mediated reduction of NO3− to NO2− (Vodovotz 1996 Biotechniques 20:390-394) and the measurement of nitrite by the Griess assay (Vodovotz 1996, supra). The absorbance values are measured at 550 nm against a standard curve of NaNO2. The procedure is described in details in Samonte et al. 2006 Agron. J. 98:168-176.
Germination tests—Germination tests compare the percentage of seeds from transgenic plants that could complete the germination process to the percentage of seeds from control plants that are treated in the same manner. Normal conditions are considered for example, incubations at 22° C. under 22-hour light 2-hour dark daily cycles. Evaluation of germination and seedling vigor is conducted between 4 and 14 days after planting. The basal media is 50% MS medium (Murashige and Skoog, 1962 Plant Physiology 15, 473-497).
Germination is checked also at unfavorable conditions such as cold (incubating at temperatures lower than 10° C. instead of 22° C.) or using seed inhibition solutions that contain high concentrations of an osmolyte such as sorbitol (at concentrations of 50 mM, 100 mM, 200 mM, 300 mM, 500 mM, and up to 1000 mM) or applying increasing concentrations of salt (of 50 mM, 100 mM, 200 mM, 300 mM, 500 mM NaCl).
The effect of the transgene on plant's vigor, growth rate, biomass, yield and/or oil content can be determined using known methods.
Plant vigor—The plant vigor can be calculated by the increase in growth parameters such as leaf area, fiber length, rosette diameter, plant fresh weight and the like per time.
Growth rate—The growth rate can be measured using digital analysis of growing plants. For example, images of plants growing in greenhouse on plot basis can be captured every 3 days and the rosette area can be calculated by digital analysis. Rosette area growth is calculated using the difference of rosette area between days of sampling divided by the difference in days between samples.
Evaluation of growth rate can be done by measuring plant biomass produced, rosette area, leaf size or root length per time (can be measured in cm2 per day of leaf area).
Relative growth area can be calculated using Formula II.

Relative growth rate area=Regression coefficient of area along time course.

Seed yield Evaluation of the seed yield per plant can be done by measuring the amount (weight or size) or quantity (i.e., number) of dry seeds produced and harvested from 8-16 plants and divided by the number of plants.
For example, the total seeds from 8-16 plants can be collected, weighted using e.g., an analytical balance and the total weight can be divided by the number of plants. Seed yield per growing area can be calculated in the same manner while taking into account the growing area given to a single plant. Increase seed yield per growing area could be achieved by increasing seed yield per plant, and/or by increasing number of plants capable of growing in a given area.
In addition, seed yield can be determined via the weight of 1000 seeds. The weight of 1000 seeds can be determined as follows: seeds are scattered on a glass tray and a picture is taken. Each sample is weighted and then using the digital analysis, the number of seeds in each sample is calculated.
The 1000 seeds weight can be calculated using formula III:

1000 Seed Weight=number of seed in sample/sample weight×1000  Formula III:

The Harvest Index can be calculated using Formula IV

Harvest Index=Average seed yield per plant/Average dry weight  Formula IV:

Grain protein concentration—Grain protein content (g grain protein m−2) is estimated as the product of the mass of grain N (g grain N m−2) multiplied by the N/protein conversion ratio of k-5.13 (Mosse 1990, supra). The grain protein concentration is estimated as the ratio of grain protein content per unit mass of the grain (g grain protein kg−1 grain).
Fiber length—Fiber length can be measured using fibrograph. The fibrograph system was used to compute length in terms of “Upper Half Mean” length. The upper half mean (UHM) is the average length of longer half of the fiber distribution. The fibrograph measures length in span lengths at a given percentage point (Hypertext Transfer Protocol://World Wide Web (dot) cottoninc (dot) com/ClassificationofCotton/?Pg=4#Length).
According to some embodiments of the invention, increased yield of corn may be manifested as one or more of the following: increase in the number of plants per growing area, increase in the number of ears per plant, increase in the number of rows per ear, number of kernels per ear row, kernel weight, thousand kernel weight (1000-weight), ear length/diameter, increase oil content per kernel and increase starch content per kernel.
As mentioned, the increase of plant yield can be determined by various parameters. For example, increased yield of rice may be manifested by an increase in one or more of the following: number of plants per growing area, number of panicles per plant, number of spikelets per panicle, number of flowers per panicle, increase in the seed filling rate, increase in thousand kernel weight (1000-weight), increase oil content per seed, increase starch content per seed, among others. An increase in yield may also result in modified architecture, or may occur because of modified architecture.
Similarly, increased yield of soybean may be manifested by an increase in one or more of the following: number of plants per growing area, number of pods per plant, number of seeds per pod, increase in the seed filling rate, increase in thousand seed weight (1000-weight), reduce pod shattering, increase oil content per seed, increase protein content per seed, among others. An increase in yield may also result in modified architecture, or may occur because of modified architecture.
Increased yield of canola may be manifested by an increase in one or more of the following: number of plants per growing area, number of pods per plant, number of seeds per pod, increase in the seed filling rate, increase in thousand seed weight (1000-weight), reduce pod shattering, increase oil content per seed, among others. An increase in yield may also result in modified architecture, or may occur because of modified architecture.
Increased yield of cotton may be manifested by an increase in one or more of the following: number of plants per growing area, number of bolls per plant, number of seeds per boll, increase in the seed filling rate, increase in thousand seed weight (1000-weight), increase oil content per seed, improve fiber length, fiber strength, among others. An increase in yield may also result in modified architecture, or may occur because of modified architecture.
Oil content—The oil content of a plant can be determined by extraction of the oil from the seed or the vegetative portion of the plant. Briefly, lipids (oil) can be removed from the plant (e.g., seed) by grinding the plant tissue in the presence of specific solvents (e.g., hexane or petroleum ether) and extracting the oil in a continuous extractor. Indirect oil content analysis can be carried out using various known methods such as Nuclear Magnetic Resonance (NMR) Spectroscopy, which measures the resonance energy absorbed by hydrogen atoms in the liquid state of the sample [See for example, Conway T F. and Earle F R., 1963, Journal of the American Oil Chemists' Society; Springer Berlin/Heidelberg, ISSN: 0003-021X (Print) 1558-9331 (Online)]; the Near Infrared (NI) Spectroscopy, which utilizes the absorption of near infrared energy (1100-2500 nm) by the sample; and a method described in WO/2001/023884, which is based on extracting oil a solvent, evaporating the solvent in a gas stream which forms oil particles, and directing a light into the gas stream and oil particles which forms a detectable reflected light.
Thus, the present invention is of high agricultural value for promoting the yield of commercially desired crops (e.g., biomass of vegetative organ such as poplar wood, or reproductive organ such as number of seeds or seed biomass).
Any of the transgenic plants described hereinabove or parts thereof may be processed to produce a feed, meal, protein or oil preparation, such as for ruminant animals.
The transgenic plants described hereinabove, which exhibit an increased oil content can be used to produce plant oil (by extracting the oil from the plant).
The plant oil (including the seed oil and/or the vegetative portion oil) produced according to the method of the invention may be combined with a variety of other ingredients. The specific ingredients included in a product are determined according to the intended use. Exemplary products include animal feed, raw material for chemical modification, biodegradable plastic, blended food product, edible oil, biofuel, cooking oil, lubricant, biodiesel, snack food, cosmetics, and fermentation process raw material. Exemplary products to be incorporated to the plant oil include animal feeds, human food products such as extruded snack foods, breads, as a food binding agent, aquaculture feeds, fermentable mixtures, food supplements, sport drinks, nutritional food bars, multi-vitamin supplements, diet drinks, and cereal foods.
According to some embodiments of the invention, the oil comprises a seed oil.
According to some embodiments of the invention, the oil comprises a vegetative portion oil.
According to some embodiments of the invention, the plant cell forms a part of a plant.
As used herein the term “about” refers to ±10%.
The terms “comprises”, “comprising”, “includes”, “including”, “having” and their conjugates mean “including but not limited to”.
The term “consisting of” means “including and limited to”.
The term “consisting essentially of” means that the composition, method or structure may include additional ingredients, steps and/or parts, but only if the additional ingredients, steps and/or parts do not materially alter the basic and novel characteristics of the claimed composition, method or structure.
As used herein, the singular form “a”, an and the include plural references unless the context clearly dictates otherwise. For example, the term “a compound” or “at least one compound” may include a plurality of compounds, including mixtures thereof.
Throughout this application, various embodiments of this invention may be presented in a range format. It should be understood that the description in range format is merely for convenience and brevity and should not be construed as an inflexible limitation on the scope of the invention. Accordingly, the description of a range should be considered to have specifically disclosed all the possible subranges as well as individual numerical values within that range. For example, description of a range such as from 1 to 6 should be considered to have specifically disclosed subranges such as from 1 to 3, from 1 to 4, from 1 to 5, from 2 to 4, from 2 to 6, from 3 to 6 etc., as well as individual numbers within that range, for example, 1, 2, 3, 4, 5, and 6. This applies regardless of the breadth of the range.
Whenever a numerical range is indicated herein, it is meant to include any cited numeral (fractional or integral) within the indicated range. The phrases “ranging/ranges between” a first indicate number and a second indicate number and “ranging/ranges from” a first indicate number “to” a second indicate number are used herein interchangeably and are meant to include the first and second indicated numbers and all the fractional and integral numerals therebetween.
As used herein the term “method” refers to manners, means, techniques and procedures for accomplishing a given task including, but not limited to, those manners, means, techniques and procedures either known to, or readily developed from known manners, means, techniques and procedures by practitioners of the chemical, pharmacological, biological, biochemical and medical arts.
It is appreciated that certain features of the invention, which are, for clarity, described in the context of separate embodiments, may also be provided in combination in a single embodiment. Conversely, various features of the invention, which are, for brevity, described in the context of a single embodiment, may also be provided separately or in any suitable subcombination or as suitable in any other described embodiment of the invention. Certain features described in the context of various embodiments are not to be considered essential features of those embodiments, unless the embodiment is inoperative without those elements.
Various embodiments and aspects of the present invention as delineated hereinabove and as claimed in the claims section below find experimental support in the following examples.
EXAMPLES
Reference is now made to the following examples, which together with the above descriptions illustrate some embodiments of the invention in a non limiting fashion.
Generally, the nomenclature used herein and the laboratory procedures utilized in the present invention include molecular, biochemical, microbiological and recombinant DNA techniques. Such techniques are thoroughly explained in the literature. See, for example, “Molecular Cloning: A laboratory Manual” Sambrook et al., (1989); “Current Protocols in Molecular Biology” Volumes I-III Ausubel, R. M., ed. (1994); Ausubel et al., “Current Protocols in Molecular Biology”, John Wiley and Sons, Baltimore, Md. (1989); Perbal, “A Practical Guide to Molecular Cloning”, John Wiley & Sons, New York (1988); Watson et al., “Recombinant DNA”, Scientific American Books, New York; Birren et al. (eds) “Genome Analysis: A Laboratory Manual Series”, Vols. 1-4, Cold Spring Harbor Laboratory Press, New York (1998); methodologies as set forth in U.S. Pat. Nos. 4,666,828; 4,683,202; 4,801,531; 5,192,659 and 5,272,057; “Cell Biology: A Laboratory Handbook”, Volumes I-III Cellis, J. E., ed. (1994); “Current Protocols in Immunology” Volumes I-III Coligan J. E., ed. (1994); Stites et al. (eds), “Basic and Clinical Immunology” (8th Edition), Appleton & Lange, Norwalk, Conn. (1994); Mishell and Shiigi (eds), “Selected Methods in Cellular Immunology”, W. H. Freeman and Co., New York (1980); available immunoassays are extensively described in the patent and scientific literature, see, for example, U.S. Pat. Nos. 3,791,932; 3,839,153; 3,850,752; 3,850,578; 3,853,987; 3,867,517; 3,879,262; 3,901,654; 3,935,074; 3,984,533; 3,996,345; 4,034,074; 4,098,876; 4,879,219; 5,011,771 and 5,281,521; “Oligonucleotide Synthesis” Gait, M. J., ed. (1984); “Nucleic Acid Hybridization” Hames, B. D., and Higgins S. J., eds. (1985); “Transcription and Translation” Hames, B. D., and Higgins S. J., Eds. (1984); “Animal Cell Culture” Freshney, R. I., ed. (1986); “Immobilized Cells and Enzymes” IRL Press, (1986); “A Practical Guide to Molecular Cloning” Perbal, B., (1984) and “Methods in Enzymology” Vol. 1-317, Academic Press; “PCR Protocols: A Guide To Methods And Applications”, Academic Press, San Diego, Calif. (1990); Marshak et al., “Strategies for Protein Purification and Characterization—A Laboratory Course Manual” CSHL Press (1996); all of which are incorporated by reference as if fully set forth herein. Other general references are provided throughout this document. The procedures therein are believed to be well known in the art and are provided for the convenience of the reader. All the information contained therein is incorporated herein by reference.
General Experimental and Bioinformatics Methods
RNA extraction—Tissues growing at various growth conditions (as described below) were sampled and RNA was extracted using TRIzol Reagent from Invitrogen [Hypertext Transfer Protocol://World Wide Web (dot) invitrogen (dot) com/content (dot)cfm?pageid=469]. Approximately 30-50 mg of tissue was taken from samples. The weighed tissues were ground using pestle and mortar in liquid nitrogen and resuspended in 500 ml of TRIzol Reagent. To the homogenized lysate, 100 ml of chloroform was added followed by precipitation using isopropanol and two washes with 75% ethanol. The RNA was eluted in 30 ml of RNase-free water. RNA samples were cleaned up using Qiagen's RNeasy minikit clean-up protocol as per the manufacturer's protocol (QIAGEN Inc, CA USA). For convenience, each micro-array expression information tissue type has received an expression Set ID.
Correlation analysis—was performed for selected genes according to some embodiments of the invention, in which the characterized parameters (measured parameters according to the correlation IDs) were used as “x axis” for correlation with the tissue transcriptom which was used as the “Y axis”. For each gene and measured parameter a correlation coefficient “R” was calculated (using Pearson correlation) along with a p-value for the significance of the correlation. When the correlation coefficient (R) between the levels of a gene's expression in a certain tissue and a phenotypic performance across ecotypes/variety/hybrid is high in absolute value (between 0.5-1), there is an association between the gene (specifically the expression level of this gene) the phenotypic characteristic (e.g., improved nitrogen use efficiency, abiotic stress tolerance, yield, growth rate and the like).
Example 1
Identifying Genes which Increase Nitrogen Use Efficiency (NUE), Fertilizer Use Efficiency (FUE), Yield, Growth Rate, Vigor, Biomass, Oil Content, Abiotic Stress Tolerance (ABST) and/or Water Use Efficiency (WUE) in Plants
The present inventors have identified polynucleotides which upregulation of expression thereof in plants increases nitrogen use efficiency (NUE), fertilizer use efficiency (FUE), yield (e.g., seed yield, oil yield, biomass, grain quantity and/or quality), growth rate, vigor, biomass, oil content, fiber yield, fiber quality, fiber length, abiotic stress tolerance (ABST) and/or water use efficiency (WUE) of a plant.
All nucleotide sequence datasets used here were originated from publicly available databases or from performing sequencing using the Solexa technology (e.g. Barley and Sorghum). Sequence data from 100 different plant species was introduced into a single, comprehensive database. Other information on gene expression, protein annotation, enzymes and pathways were also incorporated. Major databases used include:
Genomes
Arabidopsis genome [TAIR genome version 6 (Hypertext Transfer Protocol://World Wide Web (dot) arabidopsis (dot) org/)]
Rice genome [IRGSP build 4.0 (Hypertext Transfer Protocol://rgp (dot) dna (dot) affrc (dot) go (dot) jp/IRGSP/)].
Poplar [Populus trichocarpa release 1.1 from JGI (assembly release v1.0) (Hypertext Transfer Protocol://World Wide Web (dot) genome (dot) jgi-psf (dot) org/)]
Brachypodium [JGI 4× assembly, Hypertext Transfer Protocol://World Wide Web (dot) brachypodium (dot) org)]
Soybean [DOE-JGI SCP, version GlymaO (Hypertext Transfer Protocol://World Wide Web (dot) phytozome (dot) net/)]
Grape [French-Italian Public Consortium for Grapevine Genome Characterization grapevine genome (Hypertext Transfer Protocol://World Wide Web (dot) genoscope (dot) cns (dot) fr/)]
Castobean [TIGR/J Craig Venter Institute 4× assembly [(Hypertext Transfer Protocol://msc (dot) jcvi (dot) org/r_communis]
Sorghum [DOE-JGI SCP, version Sbi 1 [Hypertext Transfer Protocol://World Wide Web (dot) phytozome (dot) net/)].
Maize [Hypertext Transfer Protocol://maizesequence (dot) org/]
Cucumber [Hypertext Transfer Protocol://cucumber (dot) genomics (dot) org (dot) cn/page/cucumber/index (dot) jsp]
Tomato [Hypertext Transfer Protocol://solgenomics (dot) net/tomato/]
Cassava [Hypertext Transfer Protocol://www (dot) phytozome (dot) net/cassaya (dot) php]
Expressed EST and mRNA Sequences were Extracted from the Following Databases:
GenBank (Hypertext Transfer Protocol://World Wide Web (dot) ncbi (dot) nlm (dot) nih (dot) gov/Genbank/).
RefSeq (Hypertext Transfer Protocol://World Wide Web (dot) ncbi (dot) nlm (dot) nih (dot) gov/RefSeq/).
TAIR (Hypertext Transfer Protocol://World Wide Web (dot) arabidopsis (dot) org/).
Protein and Pathway Databases
Uniprot [Hypertext Transfer Protocol://World Wide Web (dot) uniprot (dot) org/].
AraCyc [Hypertext Transfer Protocol://World Wide Web (dot) arabidopsis (dot) org/biocyc/index (dot) jsp].
ENZYME [Hypertext Transfer Protocol://expasy (dot) org/enzyme/].
Microarray Datasets were Downloaded from:
GEO (Hypertext Transfer Protocol://World Wide Web.ncbi.nlm.nih.gov/geo/)
TAIR (Hypertext Transfer Protocol://World Wide Web.arabidopsis.org/).
Proprietary micro-array data (See WO2008/122980 and Examples 3-10 below).
QTL and SNPs Information
Gramene [Hypertext Transfer Protocol://World Wide Web (dot) gramene (dot) org/qtl/].
Panzea [Hypertext Transfer Protocol://World Wide Web (dot) panzea (dot) org/index (dot) html].
Soybean QTL: [Hypertext Transfer Protocol://World Wide Web (dot) soybeanbreederstoolbox(dot) com/].
Database Assembly—was performed to build a wide, rich, reliable annotated and easy to analyze database comprised of publicly available genomic mRNA, ESTs DNA sequences, data from various crops as well as gene expression, protein annotation and pathway, QTLs data, and other relevant information.
Database assembly is comprised of a toolbox of gene refining, structuring, annotation and analysis tools enabling to construct a tailored database for each gene discovery project. Gene refining and structuring tools enable to reliably detect splice variants and antisense transcripts, generating understanding of various potential phenotypic outcomes of a single gene. The capabilities of the “LEADS” platform of Compugen LTD for analyzing human genome have been confirmed and accepted by the scientific community [see e.g., “Widespread Antisense Transcription”, Yelin, et al. (2003) Nature Biotechnology 21, 379-85; “Splicing of Alu Sequences”, Lev-Maor, et al. (2003) Science 300 (5623), 1288-91; “Computational analysis of alternative splicing using EST tissue information”, Xie H et al. Genomics 2002], and have been proven most efficient in plant genomics as well.
EST clustering and gene assembly—For gene clustering and assembly of organisms with available genome sequence data (arabidopsis, rice, castorbean, grape, brachypodium, poplar, soybean, sorghum) the genomic LEADS version (GANG) was employed. This tool allows most accurate clustering of ESTs and mRNA sequences on genome, and predicts gene structure as well as alternative splicing events and anti-sense transcription.
For organisms with no available full genome sequence data, “expressed LEADS” clustering software was applied.
Gene annotation—Predicted genes and proteins were annotated as follows:
Sequences blast search [Hypertext Transfer Protocol://blast (dot) ncbi (dot) nlm (dot) nih (dot) gov/Blast (dot) cgi] against all plant UniProt [Hypertext Transfer Protocol://World Wide Web (dot) uniprot (dot) org/] was performed. Open reading frames of each putative transcript were analyzed and longest ORF with higher number of homologues was selected as predicted protein of the transcript. The predicted proteins were analyzed by InterPro [Hypertext Transfer Protocol://World Wide Web (dot) ebi (dot) ac (dot) uk/interpro/].
Blast against proteins from AraCyc and ENZYME databases was used to map the predicted transcripts to AraCyc pathways.
Predicted proteins from different species were compared using blast algorithm [Hypertext Transfer Protocol://World Wide Web (dot) ncbi (dot) nlm (dot) nih (dot) gov/Blast (dot) cgi] to validate the accuracy of the predicted protein sequence, and for efficient detection of orthologs.
Gene expression profiling—Several data sources were exploited for gene expression profiling, namely microarray data and digital expression profile (see below). According to gene expression profile, a correlation analysis was performed to identify genes which are co-regulated under different development stages and environmental conditions and associated with different phenotypes.
Publicly available microarray datasets were downloaded from TAIR and NCBI GEO sites, renormalized, and integrated into the database. Expression profiling is one of the most important resource data for identifying genes important for yield.
A digital expression profile summary was compiled for each cluster according to all keywords included in the sequence records comprising the cluster. Digital expression, also known as electronic Northern Blot, is a tool that displays virtual expression profile based on the EST sequences forming the gene cluster. The tool provides the expression profile of a cluster in terms of plant anatomy (e.g., the tissue/organ in which the gene is expressed), developmental stage (the developmental stages at which a gene can be found) and profile of treatment (provides the physiological conditions under which a gene is expressed such as drought, cold, pathogen infection, etc). Given a random distribution of ESTs in the different clusters, the digital expression provides a probability value that describes the probability of a cluster having a total of N ESTs to contain X ESTs from a certain collection of libraries. For the probability calculations, the following is taken into consideration: a) the number of ESTs in the cluster, b) the number of ESTs of the implicated and related libraries, c) the overall number of ESTs available representing the species. Thereby clusters with low probability values are highly enriched with ESTs from the group of libraries of interest indicating a specialized expression.
Recently, the accuracy of this system was demonstrated by Portnoy et al., 2009 (Analysis Of The Melon Fruit Transcriptome Based On 454 Pyrosequencing) in: Plant & Animal Genomes XVII Conference, San Diego, Calif. Transcriptomic analysis, based on relative EST abundance in data was performed by 454 pyrosequencing of cDNA representing mRNA of the melon fruit. Fourteen double strand cDNA samples obtained from two genotypes, two fruit tissues (flesh and rind) and four developmental stages were sequenced. GS FLX pyrosequencing (Roche/454 Life Sciences) of non-normalized and purified cDNA samples yielded 1,150,657 expressed sequence tags (ESTs) that assembled into 67,477 unigenes (32,357 singletons and 35,120 contigs). Analysis of the data obtained against the Cucurbit Genomics Database [Hypertext Transfer Protocol://World Wide Web (dot) icugi (dot) org/] confirmed the accuracy of the sequencing and assembly. Expression patterns of selected genes fitted well their qRT-PCR data.
Overall, 257 genes were identified to have a major impact on nitrogen use efficiency, fertilizer use efficiency, yield (e.g., seed yield, oil yield, grain quantity and/or quality), growth rate, vigor, biomass, oil content, fiber yield, fiber quality, fiber length, abiotic stress tolerance and/or water use efficiency when expression thereof is increased in plants. The identified genes, their curated polynucleotide and polypeptide sequences, as well as their updated sequences according to GenBank database are summarized in Table 1, hereinbelow.







TABLE 1







Identified polynucleotides for increasing nitrogen use efficiency, fertilizer use


efficiency, yield, growth rate, vigor, biomass, oil content, fiber yield, fiber quality,


fiber length, abiotic stress tolerance and/or water use efficiency of a plant















Polyp.





Polyn. SEQ
SEQ ID


Gene Name
Cluster Name
Organism
ID NO:
NO:














LNU1
arabidopsis|gb165|AT5G11630
arabidopsis
1
468


LNU2
rice|gb157.2|BI798989
rice
2
469


LNU3
rice|gb157.2|AK106493
rice
3
470


LNU4
barley|gb157.3|BI953357
barley
4
471


LNU5
barley|gb157.3|BE421774
barley
5
472


LNU6
soybean|gb166|BI942460
soybean
6
473


LNU7
soybean|gb166|CD398173
soybean
7
474


LNU8
arabidopsis|gb165|AT3G18200
arabidopsis
8
475


LNU9
rice|gb157.2|AU066136
rice
9
476


LNU10
rice|gb157.2|CB678538
rice
10
477


LNU11
rice|gb157.2|CB641645
rice
11
478


LNU12
rice|gb157.2|Y11415
rice
12
479


LNU13
rice|gb157.2|BI805840
rice
13
480


LNU14
arabidopsis|gb165|AT2G37860
arabidopsis
14
481


LNU15
arabidopsis|gb165|AT3G07420
arabidopsis
15
482


LNU17
rice|gb157.2|BF430745
rice
16
483


LNU19
rice|gb157.2|CB642397
rice
17
484


LNU20
tomato|gb164|BG131270
tomato
18
485


LNU23
arabidopsis|gb165|AT1G23120
arabidopsis
19
486


LNU24
arabidopsis|gb165|AT1G33110
arabidopsis
20
487


LNU25
sorghum|gb161.xeno|BE355836
sorghum
21
488


LNU27
barley|gb157.3|BE196470
barley
22
489


LNU28
barley|gb157.3|AL500488
barley
23
490


LNU29
tomato|gb164|AI487919
tomato
24
491


LNU32
sorghum|gb161.xeno|AW671708
sorghum
25
492


LNU33
soybean|gb166|CD408405
soybean
26
493


LNU34
rice|gb157.2|AU030308
rice
27
494


LNU35
wheat|gb164|BE442655
wheat
28
495


LNU36
soybean|gb168|BE347766
soybean
29
496


LNU37
rice|gb157.2|CA767513
rice
30
497


LNU40
rice|gb157.2|OSU76004
rice
31
498


LNU43
soybean|gb166|AW349541
soybean
32
499


LNU44
soybean|gb166|GMU12150
soybean
33
500


LNU45
soybean|gb166|AW508359
soybean
34
501


LNU46
soybean|gb166|AW348273
soybean
35
502


LNU48
rice|gb157.3|BI806333
rice
36
503


LNU50
rice|gb157.3|AK070604
rice
37
504


LNU51
rice|gb157.3|AA751405
rice
38
505


LNU52
rice|gb157.3|AF042333
rice
39
506


LNU53
soybean|gb168|CA782562
soybean
40
507


LNU54
soybean|gb168|BF518437
soybean
41
508


LNU55
soybean|gb168|BQ080255
soybean
42
509


LNU56
soybean|gb168|BE352719
soybean
43
510


LNU57
wheat|gb164|BE405851
wheat
44
511


LNU58
wheat|gb164|BE585823
wheat
45
512


LNU59
wheat|gb164|BE515786
wheat
46
513


LNU60
wheat|gb164|BQ901296
wheat
47
514


LNU61
wheat|gb164|BE498157
wheat
48
515


LNU63
wheat|gb164|BF429186
wheat
49
516


LNU64
wheat|gb164|BU100011
wheat
50
517


LNU65
rice|gb157.3|C28856
rice
51
518


LNU67
rice|gb157.3|AA749717
rice
52
519


LNU68
rice|gb157.3|BM421254
rice
53
520


LNU69
rice|gb157.3|AF458088
rice
54
521


LNU70
rice|gb170|OS11G48080
rice
55
522


LNU71
rice|gb157.3|AF210325
rice
56
523


LNU72
barley|gb157.3|BI954541
barley
57
524


LNU73
rice|gb157.3|AA754527
rice
58
525


LNU74
poplar|gb170|AI164893
poplar
59
526


LNU75
soybean|gb168|AW690409
soybean
60
527


LNU76
rice|gb157.3|AA752216
rice
61
528


LNU79
cotton|gb164|BF272356
cotton
62
529


LNU81
barley|gb157.3|BE421380
barley
63
530


LNU82
rice|gb157.3|AU031357
rice
64
531


LNU83
soybean|gb168|AW471606
soybean
65
532


LNU84
sorghum|gb161.xeno|AI714503
sorghum
66
533


LNU85
sorghum|gb161.xeno|AI941787
sorghum
67
534


LNU86
maize|gb169.2|AI941972
maize
68
535


LNU87
sorghum|gb161.crp|BM325119
sorghum
69
536


LNU89
wheat|gb164|BQ236209
wheat
70
537


LNU94
tomato|gb164|BF113903
tomato
71
538


LNU95
soybean|gb168|BQ741102
soybean
72
539


LNU96
rice|gb157.3|AA751884
rice
73
540


LNU98
maize|gb164|AI947517
maize
74
541


LNU100
cotton|gb164|AI055197
cotton
75
542


LNU101
rice|gb157.3|NM001061106
rice
76
543


LNU104
soybean|gb168|BQ610458
soybean
77
544


LNU105
wheat|gb164|BG606394
wheat
78
545


LNU106
wheat|gb164|BF201718
wheat
79
546


LNU107
rice|gb157.3|BE040237
rice
80
547


LNU109
rice|gb157.3|AU032452
rice
81
548


LNU110
rice|gb157.3|CA755769
rice
82
549


LNU112
rice|gb157.3|AU057246
rice
83
550


LNU113
maize|gb164|AA054793
maize
84
551


LNU114
rice|gb157.3|AA752410
rice
85
552


LNU115
rice|gb157.3|CB635059
rice
86
553


LNU116
rice|gb170|OS10G28240
rice
87
554


LNU117
rice|gb157.3|AU081366
rice
88
555


LNU118
rice|gb157.3|AU098344
rice
89
556


LNU119
rice|gb157.3|AK063703
rice
90
557


LNU120
rice|gb170|OS02G37380
rice
91
558


LNU121
rice|gb157.3|AU166339
rice
92
559


LNU122
rice|gb157.3|BI813219
rice
93
560


LNU123
arabidopsis|gb165|AT1G63850
arabidopsis
94
561


LNU124
arabidopsis|gb165|AT5G54130T2
arabidopsis
95
562


LNU126
arabidopsis|gb165|AT5G58770
arabidopsis
96
563


LNU127
arabidopsis|gb165|AT5G61820
arabidopsis
97
564


LNU128
arabidopsis|gb165|AT1G21690
arabidopsis
98
565


LNU129
arabidopsis|gb165|AT1G53450
arabidopsis
99
566


LNU130
arabidopsis|gb165|AT1G76560
arabidopsis
100
567


LNU131
arabidopsis|gb165|AT2G41950
arabidopsis
101
568


LNU132
arabidopsis|gb165|AT1G68440
arabidopsis
102
569


LNU133
arabidopsis|gb165|AT1G72020
arabidopsis
103
570


LNU134
arabidopsis|gb165|AT4G12000
arabidopsis
104
571


LNU135
arabidopsis|gb165|AT4G38800
arabidopsis
105
572


LNU136
arabidopsis|gb165|AT3G26440
arabidopsis
106
573


LNU138
barley|gb157.3|AL500952
barley
107
574


LNU140
arabidopsis|gb165|AT1G62430
arabidopsis
108
575


LNU141
wheat|gb164|BE604062
wheat
109
576


LNU142
barley|gb157.3|AJ472814
barley
110
577


LNU143
cotton|gb164|BG443936
cotton
111
578


LNU146
rice|gb157.3|AA754467
rice
112
579


LNU147
cotton|gb164|DT462051
cotton
113
580


LNU148
soybean|gb168|CD394513
soybean
114
581


LNU149
rice|gb157.3|AK110423
rice
115
582


LNU150
cotton|gb164|AW186819
cotton
116
583


LNU153
rice|gb157.3|AU166793
rice
117
584


LNU154
cotton|gb164|AI054586
cotton
118
585


LNU155
cotton|gb164|CO101542
cotton
119
586


LNU157
soybean|gb166|CF921687
soybean
120
587


LNU158
cotton|gb164|CO082594
cotton
121
588


LNU161
soybean|gb168|BE661583
soybean
122
589


LNU168
sorghum|gb161.crp|AW927746
sorghum
123
590


LNU170
arabidopsis|gb165|AT5G40060
arabidopsis
124
591


LNU171
barley|gb157.3|AL501130
barley
125
592


LNU172
barley|gb157.3|BI777246
barley
126
593


LNU173
barley|gb157.3|BE437951
barley
127
594


LNU175
arabidopsis|gb165|AT4G28290
arabidopsis
128
595


LNU176
rice|gb157.3|AU062564
rice
129
596


LNU177
arabidopsis|gb165|AT1G67740
arabidopsis
130
597


LNU178
arabidopsis|gb165|AT1G18300
arabidopsis
131
598


LNU179
arabidopsis|gb165|AT1G53560
arabidopsis
132
599


LNU180
arabidopsis|gb165|AT1G70230
arabidopsis
133
600


LNU181
arabidopsis|gb165|AT3G57940
arabidopsis
134
601


LNU182
arabidopsis|gb165|AT3G08980
arabidopsis
135
602


LNU183
arabidopsis|gb165|AT1G58340
arabidopsis
136
603


LNU184
arabidopsis|gb165|AT3G52230
arabidopsis
137
604


LNU185
arabidopsis|gb165|AT3G63160
arabidopsis
138
605


LNU186
arabidopsis|gb165|AT2G03350
arabidopsis
139
606


LNU187
arabidopsis|gb165|AT5G01540
arabidopsis
140
607


LNU188
soybean|gb166|BU547183
soybean
141
608


LNU189
rice|gb157.3|BE230329
rice
142
609


LNU190
canola|gb161|DY007527
canola
143
610


LNU191
rice|gb157.3|CA753062
rice
144
611


LNU192
rice|gb157.3|BE040846
rice
145
612


LNU196
rice|gb157.3|BI809290
rice
146
613


LNU198
cotton|gb164|CO078561
cotton
147
614


LNU200
tomato|gb164|BG791292
tomato
148
615


LNU202
sorghum|gb161.xeno|BE362397
sorghum
149
616


LNU206
arabidopsis|gb165|AT2G31890
arabidopsis
150
617


LNU207
arabidopsis|gb165|AT1G70260
arabidopsis
151
618


LNU210
arabidopsis|gb165|AT3G61060
arabidopsis
152
619


LNU211
arabidopsis|gb165|AT5G06270
arabidopsis
153
620


LNU212
arabidopsis|gb165|AT1G28400
arabidopsis
154
621


LNU213
arabidopsis|gb165|AT5G11690
arabidopsis
155
622


LNU214
arabidopsis|gb165|AT1G19020
arabidopsis
156
623


LNU215
arabidopsis|gb165|AT1G08570
arabidopsis
157
624


LNU216
rice|gb157.3|BI804955
rice
158
625


LNU217
rice|gb157.3|AT003632
rice
159
626


LNU218
arabidopsis|gb165|AT5G35460
arabidopsis
160
627


LNU219
arabidopsis|gb165|AT4G19400
arabidopsis
161
628


LNU220
rice|gb157.3|AW155256
rice
162
629


LNU222
wheat|gb164|BE400657
wheat
163
630


LNU223
rice|gb157.3|BI798260
rice
164
631


LNU224
barley|gb157.3|BF628111
barley
165
632


LNU225
arabidopsis|gb165|AT4G27050
arabidopsis
166
633


LNU228
barley|gb157.3|BF622377
barley
167
634


LNU229
tomato|gb164|AI484048
tomato
168
635


LNU230
rice|gb157.3|AU091786
rice
169
636


LNU232
rice|gb157.2|CA754695
rice
170
637


LNU234
arabidopsis|gb165|AT1G22140
arabidopsis
171
638


LNU235
arabidopsis|gb165|AT1G33590
arabidopsis
172
639


LNU236
cotton|gb164|AI728962
cotton
173
640


LNU239
rice|gb157.2|BE530901
rice
174
641


LNU240
barley|gb157.3|AV914239
barley
175
642


LNU241
rice|gb157.2|CA756471
rice
176
643


LNU242
arabidopsis|gb165|AT4G01650
arabidopsis
177
644


LNU243
barley|gb157.3|BQ467891
barley
178
645


LNU244
barley|gb157.3|AV932151
barley
179
646


LNU245
tomato|gb164|AI486625
tomato
180
647


LNU246
tomato|gb164|AI896232
tomato
181
648


LNU247
arabidopsis|gb165|AT5G15170
arabidopsis
182
649


LNU249
arabidopsis|gb165|AT5G04980
arabidopsis
183
650


LNU250
arabidopsis|gb165|AT1G30860
arabidopsis
184
651


LNU251
arabidopsis|gb165|AT4G04940
arabidopsis
185
652


LNU253
soybean|gb166|CD407540
soybean
186
653


LNU254
arabidopsis|gb165|AT1G47670
arabidopsis
187
654


LNU255
arabidopsis|gb165|AT2G42760
arabidopsis
188
655


LNU256
arabidopsis|gb165|AT2G43920
arabidopsis
189
656


LNU257
arabidopsis|gb165|AT3G12090
arabidopsis
190
657


LNU258
arabidopsis|gb165|AT4G37330
arabidopsis
191
658


LNU260
arabidopsis|gb165|AT5G07020
arabidopsis
192
659


LNU261
arabidopsis|gb165|AT5G48470
arabidopsis
193
660


LNU262
arabidopsis|gb165|AT5G49900
arabidopsis
194
661


LNU263
barley|gb157.3|AL502706
barley
195
662


LNU265
maize|gb164|AI396555
maize
196
663


LNU265
maize|gb164|AI396555
maize
196
705


LNU266
maize|gb164|AI438792
maize
197
664


LNU267
maize|gb164|AI973407
maize
198
665


LNU268
maize|gb164|BE123241
maize
199
666


LNU271
rice|gb157.2|AU089771
rice
200
667


LNU274
rice|gb157.3|BI305442
rice
201
668


LNU275
rice|gb170|OS09G35600
rice
202
669


LNU276
rice|gb157.3|AA753720
rice
203
670


LNU277
rice|gb157.3|CV723478
rice
204
671


LNU278
sorghum|gb161.xeno|AW671348
sorghum
205
672


LNU279
sorghum|gb161.xeno|AW677534
sorghum
206
673


LNU280
sorghum|gb161.crp|BM660677
sorghum
207
674


LNU282
soybean|gb166|BI968975
soybean
208
675


LNU284
soybean|gb166|CA851742
soybean
209
676


LNU287
soybean|gb168|CD394819
soybean
210
677


LNU288
tomato|gb164|BG134658
tomato
211
678


LNU289
tomato|gb164|BG123295
tomato
212
679


LNU222_H6
sorghum|gb161.crp|AW678240
sorghum
213
680


LNU125
arabidopsis|gb165|AT4G04925
arabidopsis
214
—


LNU201
rice|gb157.3|BI801545
rice
215
—


LNU233
rice|gb157.3|AK107825
rice
216
—


LNU3
rice|gb170|OS03G51530
rice
217
470


LNU29
tomato|gb164|AI487919
tomato
218
681


LNU33
soybean|gb168|AL374064
soybean
219
493


LNU35
wheat|gb164|BE442655
wheat
220
682


LNU36
soybean|gb168|BE347766
soybean
221
496


LNU53
soybean|gb168|CA782562
soybean
222
683


LNU55
soybean|gb168|BQ080255
soybean
223
684


LNU57
wheat|gb164|BE405851
wheat
224
685


LNU60
wheat|gb164|BQ901296
wheat
225
686


LNU74
poplar|gb157.2|AI164893
poplar
226
526


LNU83
soybean|gb168|AW471606
soybean
227
687


LNU89
wheat|gb164|BQ236209
wheat
228
688


LNU101
rice|gb157.3|NM001061106
rice
229
689


LNU105
wheat|gb164|BG606394
wheat
230
690


LNU113
maize|gb169.2|AA054793
maize
231
691


LNU114
rice|gb157.3|AA752410
rice
232
692


LNU115
rice|gb157.3|CB635059
rice
233
693


LNU123
arabidopsis|gb165|AT1G63850
arabidopsis
234
561


LNU126
arabidopsis|gb165|AT5G58770
arabidopsis
235
563


LNU143
cotton|gb164|BG443936
cotton
236
694


LNU147
cotton|gb164|DT462051
cotton
237
580


LNU148
soybean|gb168|CD394513
soybean
238
695


LNU158
cotton|gb164|CO082594
cotton
239
696


LNU170
arabidopsis|gb165|AT5G40060
arabidopsis
240
697


LNU190
canola|gb161|DY007527
canola
241
610


LNU192
rice|gb157.3|BE040846
rice
242
698


LNU198
cotton|gb164|CO078561
cotton
243
614


LNU200
tomato|gb164|BG791292
tomato
244
699


LNU202
sorghum|gb161.xeno|BE362397
sorghum
245
700


LNU229
tomato|gb164|AI484048
tomato
246
701


LNU236
cotton|gb164|AI728962
cotton
247
640


LNU240
barley|gb157.3|AV914239
barley
248
702


LNU241
rice|gb170|OS12G40330
rice
249
643


LNU242
arabidopsis|gb165|AT4G01650
arabidopsis
250
703


LNU243
barley|gb157.3|BQ467891
barley
251
645


LNU244
barley|gb157.3|AV932151
barley
252
704


LNU249
arabidopsis|gb165|AT5G04980
arabidopsis
253
650


LNU257
arabidopsis|gb165|AT3G12090
arabidopsis
254
657


LNU266
maize|gb164|AI438792
maize
255
706


LNU289
tomato|gb164|BG123295
tomato
256
679


LNU222_H6
sorghum|gb161.crp|AW678240
sorghum
257
680





Table 1.


“Polyp.” = polypeptide;


“Polyn.”—Polynucleotide.






Example 2
Identification of Homologous Sequences that Increase Nitrogen use efficiency, fertilizer use efficiency, yield, Growth Rate, Vigor, Biomass, Oil Content, Abiotic Stress Tolerance and/or Water Use Efficiency in Plants
The concepts of orthology and paralogy have recently been applied to functional characterizations and classifications on the scale of whole-genome comparisons. Orthologs and paralogs constitute two major types of homologs: The first evolved from a common ancestor by specialization, and the latter is related by duplication events. It is assumed that paralogs arising from ancient duplication events are likely to have diverged in function while true orthologs are more likely to retain identical function over evolutionary time.
To further investigate and identify putative orthologs of the genes affecting nitrogen use efficiency, fertilizer use efficiency, yield (e.g., seed yield, oil yield, biomass, grain quantity and/or quality), growth rate, vigor, biomass, oil content, abiotic stress tolerance and/or water use efficiency, all sequences were aligned using the BLAST (/Basic Local Alignment Search Tool/). Sequences sufficiently similar were tentatively grouped. These putative orthologs were further organized under a Phylogram—a branching diagram (tree) assumed to be a representation of the evolutionary relationships among the biological taxa. Putative ortholog groups were analyzed as to their agreement with the phylogram and in cases of disagreements these ortholog groups were broken accordingly. Expression data was analyzed and the EST libraries were classified using a fixed vocabulary of custom terms such as developmental stages (e.g., genes showing similar expression profile through development with up regulation at specific stage, such as at the seed filling stage) and/or plant organ (e.g., genes showing similar expression profile across their organs with up regulation at specific organs such as seed). The annotations from all the ESTs clustered to a gene were analyzed statistically by comparing their frequency in the cluster versus their abundance in the database, allowing the construction of a numeric and graphic expression profile of that gene, which is termed “digital expression”. The rationale of using these two complementary methods with methods of phenotypic association studies of QTLs, SNPs and phenotype expression correlation is based on the assumption that true orthologs are likely to retain identical function over evolutionary time. These methods provide different sets of indications on function similarities between two homologous genes, similarities in the sequence level—identical amino acids in the protein domains and similarity in expression profiles.
The search and identification of homologous genes involves the screening of sequence information available, for example, in public databases, which include but are not limited to the DNA Database of Japan (DDBJ), Genbank, and the European Molecular Biology Laboratory Nucleic Acid Sequence Database (EMBL) or versions thereof or the MIPS database. A number of different search algorithms have been developed, including but not limited to the suite of programs referred to as BLAST programs. There are five implementations of BLAST, three designed for nucleotide sequence queries (BLASTN, BLASTX, and TBLASTX) and two designed for protein sequence queries (BLASTP and TBLASTN) (Coulson, Trends in Biotechnology: 76-80, 1994; Birren et al., Genome Analysis, I: 543, 1997). Such methods involve alignment and comparison of sequences. The BLAST algorithm calculates percent sequence identity and performs a statistical analysis of the similarity between the two sequences. The software for performing BLAST analysis is publicly available through the National Centre for Biotechnology Information. Other such software or algorithms are GAP, BESTFIT, FASTA and TFASTA. GAP uses the algorithm of Needleman and Wunsch (J. Mol. Biol. 48: 443-453, 1970) to find the alignment of two complete sequences that maximizes the number of matches and minimizes the number of gaps.
The homologous genes may belong to the same gene family. The analysis of a gene family may be carried out using sequence similarity analysis. To perform this analysis one may use standard programs for multiple alignments e.g. Clustal W. A neighbor-joining tree of the proteins homologous to the genes of some embodiments of the invention may be used to provide an overview of structural and ancestral relationships. Sequence identity may be calculated using an alignment program as described above. It is expected that other plants will carry a similar functional gene (orthologue) or a family of similar genes and those genes will provide the same preferred phenotype as the genes presented here. Advantageously, these family members may be useful in the methods of some embodiments of the invention. Example of other plants include, but not limited to, barley (Hordeum vulgare), Arabidopsis (Arabidopsis thaliana), maize (Zea mays), cotton (Gossypium), Oilseed rape (Brassica napus), Rice (Oryza sativa), Sugar cane (Saccharum officinarum), Sorghum (Sorghum bicolor), Soybean (Glycine max), Sunflower (Helianthus annuus), Tomato (Lycopersicon esculentum) and Wheat (Triticum aestivum)
The above-mentioned analyses for sequence homology is preferably carried out on a full-length sequence, but may also be based on a comparison of certain regions such as conserved domains. The identification of such domains, would also be well within the realm of the person skilled in the art and would involve, for example, a computer readable format of the nucleic acids of some embodiments of the invention, the use of alignment software programs and the use of publicly available information on protein domains, conserved motifs and boxes. This information is available in the PRODOM (Hypertext Transfer Protocol://World Wide Web (dot) biochem (dot) ucl (dot) ac (dot) uk/bsm/dbbrowser/protocol/prodomqry (dot) html), PIR (Hypertext Transfer Protocol://pir (dot) Georgetown (dot) edu/) or Pfam (Hypertext Transfer Protocol://World Wide Web (dot) sanger (dot) ac (dot) uk/Software/Pfam/) database. Sequence analysis programs designed for motif searching may be used for identification of fragments, regions and conserved domains as mentioned above. Preferred computer programs include, but are not limited to, MEME, SIGNALSCAN, and GENESCAN.
A person skilled in the art may use the homologous sequences provided herein to find similar sequences in other species and other organisms. Homologues of a protein encompass, peptides, oligopeptides, polypeptides, proteins and enzymes having amino acid substitutions, deletions and/or insertions relative to the unmodified protein in question and having similar biological and functional activity as the unmodified protein from which they are derived. To produce such homologues, amino acids of the protein may be replaced by other amino acids having similar properties (conservative changes, such as similar hydrophobicity, hydrophilicity, antigenicity, propensity to form or break a-helical structures or 3-sheet structures). Conservative substitution Tables are well known in the art [see for example Creighton (1984) Proteins. W.H. Freeman and Company]. Homologues of a nucleic acid encompass nucleic acids having nucleotide substitutions, deletions and/or insertions relative to the unmodified nucleic acid in question and having similar biological and functional activity as the unmodified nucleic acid from which they are derived.
Polynucleotides and polypeptides with significant homology to the identified genes described in Table 1 (Example 1 above) were identified from the databases using BLAST software using the Blastp and tBlastn algorithms. The query polypeptide sequences were SEQ ID NOs: 468-706 (which are encoded by the polynucleotides SEQ ID NOs:1-257, shown in Table 1 above) and SEQ ID NOs:707-784 (which are encoded by the cloned genes SEQ ID NOs:258-467, shown in Table 59 and the identified homologous sequences are provided in Table 2, below.







TABLE 2







Homologues of the identified genes/polypeptides for increasing nitrogen use


efficiency, fertilizer use efficiency, yield, seed yield, growth rate, vigor, biomass, oil


content, fiber yield, fiber quality, fiber length, abiotic stress tolerance and/or water


use efficiency of a plant

















Hom.







Polyp.
to




Polyn.
Hom. to

SEQ
SEQ
%



SEQ ID
Gene

ID
ID
global



NO:
Name
Cluster Name
NO:
NO:
identity
Algor.
















785
LNU1
canola|10v1|CD822833
3042
468
82.8
globlastp


786
LNU1
canola|gb161|CD822833
3042
468
82.8
globlastp


787
LNU1
radish|gb164|EW724281
3043
468
82.8
globlastp


788
LNU1
canola|10v1|CD819354
3044
468
81.9
globlastp


789
LNU1
b_oleracea|gb161|EE534144
3045
468
81.7
globlastp


790
LNU1
radish|gb164|FD530209
3046
468
81.7
globlastp


791
LNU1
b_rapa|gb162|EX016038
3047
468
81.1
globlastp


792
LNU1
b_oleracea|gb161|DY026134
3048
468
80.9
globlastp


793
LNU1
canola|gb161|CD819354
3049
468
80.9
globlastp


794
LNU2
leymus|gb166|EG388463
3050
469
85
globlastp


795
LNU2
wheat|gb164|BE427374
3051
469
85
globlastp


796
LNU2
barley|gb157SOLEXA|AL507333
3052
469
84.5
globlastp


797
LNU2
maize|gb170|AI691484
3053
469
83.6
globlastp


798
LNU2
sugarcane|10v1|AA525691
3054
469
83.3
globlastp


799
LNU2
sugarcane|gb157.3|AA525691
3055
469
82.9
globlastp


800
LNU2
sorghum|09v1|SB10G006850
3056
469
82.5
globlastp


801
LNU2
sorghum|gb161.crp|AI881329
3056
469
82.5
globlastp


802
LNU2
switchgrass|gb167|FE630265
3057
469
81.9
globlastp


803
LNU2
maize|gb170|AI967022
3058
469
81.1
globlastp


804
LNU2
brachypodium|09v1|DV476481
3059
469
81
globlastp


805
LNU2
brachypodium|gb169|BE427374
3059
469
81
globlastp


806
LNU5
wheat|gb164|BE428356
3060
472
98.1
globlastp


807
LNU5
wheat|gb164|BQ806386
3061
472
97.7
globlastp


808
LNU5
leymus|gb166|EG389542
3062
472
97.2
globlastp


809
LNU5
oat|10v1|GR347048
3063
472
90.3
globlastp


810
LNU6
soybean|gb168|BE660452
3064
473
90.3
globlastp


811
LNU6
bean|gb167|CA901464
3065
473
85.8
globlastp


812
LNU6
cowpea|gb166|FF389080
3066
473
83.2
globlastp


813
LNU6
liquorice|gb171|FS239924
3067
473
80.9
globlastp


814
LNU6
peanut|gb167|AY639025
3068
473
80.6
globlastp


815
LNU6
peanut|gb171|AY639025
3069
473
80.6
globlastp


816
LNU6
lotus|09v1|LLBF177846
3070
473
80.3
globlastp


817
LNU7
chickpea|09v2|GR392103
3071
474
98.4
globlastp


817
LNU27
chickpea|09v2|GR392103
3071
489
80
glotblastn


818
LNU7
liquorice|gb171|FS238627
3072
474
98.4
globlastp


818
LNU27
liquorice|gb171|FS238627
3072
489
80
glotblastn


819
LNU7
liquorice|gb171|FS260230
3072
474
98.4
globlastp


819
LNU27
liquorice|gb171|FS260230
3072
489
80
glotblastn


820
LNU7
cacao|gb167|CU470501
3073
474
98.4
globlastp


820
LNU27
cacao|gb167|CU470501
3073
489
80
glotblastn


821
LNU7
cassava|09v1|DV445520
3072
474
98.4
globlastp


821
LNU27
cassava|09v1|DV445520
3072
489
80
glotblastn


822
LNU7
cassava|gb164|DV445520
3072
474
98.4
globlastp


822
LNU27
cassava|gb164|DV445520
3072
489
80
glotblastn


823
LNU7
cotton|gb164|BE054360
3073
474
98.4
globlastp


823
LNU27
cotton|gb164|BE054360
3073
489
80
glotblastn


824
LNU7
cotton|gb164|ES793421
3073
474
98.4
globlastp


824
LNU27
cotton|gb164|ES793421
3073
489
80
glotblastn


825
LNU7
medicago|09v1|AL377934
3071
474
98.4
globlastp


825
LNU27
medicago|09v1|AL377934
3071
489
80
glotblastn


826
LNU7
medicago|gb157.2|AL377934
3071
474
98.4
globlastp


826
LNU27
medicago|gb157.2|AL377934
3071
489
80
glotblastn


827
LNU7
soybean|gb168|AI967471
3072
474
98.4
globlastp


827
LNU27
soybean|gb168|AI967471
3072
489
80
glotblastn


828
LNU7
soybean|gb168|AW348687
3072
474
98.4
globlastp


828
LNU27
soybean|gb168|AW348687
3072
489
80
glotblastn


829
LNU7
chickpea|09v2|FE668992
3074
474
96.72
glotblastn


829
LNU27
chickpea|09v2|FE668992
3074
489
80
glotblastn


830
LNU7
pigeonpea|gb171|GR471306
3075
474
96.7
globlastp


830
LNU27
pigeonpea|gb171|GR471306
3075
489
80
glotblastn


831
LNU7
bean|gb167|CA897804
3076
474
96.7
globlastp


831
LNU27
bean|gb167|CA897804
3076
489
80
glotblastn


832
LNU7
bean|gb167|FD780529
3076
474
96.7
globlastp


832
LNU27
bean|gb167|FD780529
3076
489
80
glotblastn


833
LNU7
castorbean|09v1|XM002519154
3077
474
96.7
globlastp


833
LNU27
castorbean|09v1|XM002519154
3077
489
80.3
globlastp


834
LNU7
castorbean|gb160|MDL29889M003271
3077
474
96.7
globlastp


834
LNU27
castorbean|gb160|MDL29889M003271
3077
489
80.3
globlastp


835
LNU7
cowpea|gb166|FC459169
3076
474
96.7
globlastp


835
LNU27
cowpea|gb166|FC459169
3076
489
80
glotblastn


836
LNU7
cowpea|gb166|FF384333
3076
474
96.7
globlastp


836
LNU27
cowpea|gb166|FF384333
3076
489
80
glotblastn


837
LNU7
soybean|gb168|CD391265
3076
474
96.7
globlastp


837
LNU27
soybean|gb168|CD391265
3076
489
80
glotblastn


838
LNU7
heritiera|10v1|SRR005794S0008204
3078
474
95.1
globlastp


838
LNU27
heritiera|10v1|SRR005794S0008204
3078
489
80
glotblastn


839
LNU7
cacao|gb167|CU492958
3079
474
95.1
globlastp


840
LNU7
cotton|gb164|BF274438
3080
474
95.1
globlastp


840
LNU27
cotton|gb164|BF274438
3080
489
80
glotblastn


841
LNU7
kiwi|gb166|FG419099
3081
474
95.1
globlastp


842
LNU7
papaya|gb165|EX229339
3082
474
95.1
globlastp


842
LNU27
papaya|gb165|EX229339
3082
489
80
glotblastn


843
LNU7
medicago|09v1|LLCO511931
3083
474
93.4
globlastp


844
LNU7
bruguiera|gb166|BP940274
3084
474
93.4
globlastp


844
LNU27
bruguiera|gb166|BP940274
3084
489
80.3
globlastp


845
LNU7
cassava|09v1|CK646795
3085
474
93.4
globlastp


846
LNU7
grape|gb160|BQ792422
3086
474
93.4
globlastp


847
LNU7
kiwi|gb166|FG400845
3087
474
93.4
globlastp


848
LNU7
kiwi|gb166|FG434680
3088
474
93.4
globlastp


849
LNU7
lotus|09v1|AI967471
3089
474
93.4
globlastp


850
LNU7
lotus|gb157.2|AI967471
3089
474
93.4
globlastp


851
LNU7
medicago|09v1|AW171649
3090
474
93.4
globlastp


852
LNU7
medicago|gb157.2|AW171649
3090
474
93.4
globlastp


853
LNU7
poplar|gb170|AI164188
3091
474
93.4
globlastp


854
LNU7
poplar|10v1|BI127039
3092
474
93.4
globlastp


855
LNU7
poplar|gb170|BI127039
3092
474
93.4
globlastp


856
LNU7
jatropha|09v1|FM887189
3093
474
91.8
globlastp


857
LNU7
rhizophora|10v1|SRR005793S0035789
3094
474
91.8
globlastp


857
LNU27
rhizophora|10v1|SRR005793S0035789
3094
489
80.3
globlastp


858
LNU7
tea|10v1|CV014009
3095
474
91.8
globlastp


859
LNU7
bean|gb167|FD789886
3096
474
91.8
glotblastn


860
LNU7
chestnut|gb170|SRR006295S0000269
3097
474
91.8
globlastp


861
LNU7
cotton|gb164|BF270755
3098
474
91.8
globlastp


861
LNU27
cotton|gb164|BF270755
3098
489
80
glotblastn


862
LNU7
peanut|gb167|CX128150
3099
474
91.8
globlastp


863
LNU7
peanut|gb171|CX128150
3099
474
91.8
globlastp


864
LNU7
peanut|gb171|EE126662
3099
474
91.8
globlastp


865
LNU7
poplar|10v1|AI164188
3100
474
91.8
globlastp


866
LNU7
spurge|gb161|BG354130
3101
474
91.8
globlastp


866
LNU27
spurge|gb161|BG354130
3101
489
81
globlastp


867
LNU7
walnuts|gb166|CV196459
3102
474
91.8
globlastp


868
LNU7
eucalyptus|gb166|CU394883
3103
474
90.3
globlastp


868
LNU27
eucalyptus|gb166|CU394883
3103
489
80
glotblastn


869
LNU7
grape|gb160|EC927944
3104
474
90.3
globlastp


870
LNU7
cleome_gynandra|10v1|SRR015532S0000915
3105
474
90.2
globlastp


871
LNU7
cleome_gynandra|10v1|SRR015532S0002395
3106
474
90.2
globlastp


872
LNU7
cleome_spinosa|10v1|SRR015531S0004899
3107
474
90.2
globlastp


873
LNU7
cleome_spinosa|10v1|SRR015531S0010059
3105
474
90.2
globlastp


874
LNU7
cleome_spinosa|10v1|SRR015531S0015400
3108
474
90.2
globlastp


875
LNU7
eggplant|10v1|FS005478
3109
474
90.2
globlastp


875
LNU27
eggplant|10v1|FS005478
3109
489
80
globlastp


876
LNU7
pepper|gb171|GD054049
3110
474
90.2
globlastp


876
LNU27
pepper|gb171|GD054049
3110
489
81.7
globlastp


877
LNU7
catharanthus|gb166|DT527689
3111
474
90.2
globlastp


878
LNU7
catharanthus|gb166|EG556443
3111
474
90.2
globlastp


879
LNU7
oak|gb170|SRR006309S0014100
3112
474
90.2
globlastp


880
LNU7
rice|gb170|OS01G19840
3113
474
90.2
globlastp


880
LNU27
rice|gb170|OS01G19840
3113
489
85
globlastp


881
LNU7
rice|gb170|OS05G28750
3113
474
90.2
globlastp


881
LNU27
rice|gb170|OS05G28750
3113
489
85
globlastp


882
LNU7
walnuts|gb166|EL900862
3114
474
90.2
globlastp


883
LNU7
citrus|gb166|BQ623885
3115
474
90.16
glotblastn


884
LNU7
amborella|gb166|CK759343
3116
474
88.7
globlastp


885
LNU7
avocado|10v1|CK767358
3117
474
88.7
globlastp


885
LNU27
avocado|10v1|CK767358
3117
489
80
glotblastn


886
LNU7
avocado|gb164|CK767358
3117
474
88.7
globlastp


886
LNU27
avocado|gb164|CK767358
3117
489
80
glotblastn


887
LNU7
nuphar|gb166|CV004221
3118
474
88.7
globlastp


888
LNU7
tomato|gb164|BG126482
3119
474
88.52
glotblastn


888
LNU27
tomato|gb164|BG126482
3119
489
80
glotblastn


889
LNU7
ipomoea_batatas|10v1|BU691209
3120
474
88.5
globlastp


890
LNU7
ipomoea_nil|10v1|BJ567189
3120
474
88.5
globlastp


891
LNU7
lotus|09v1|CRPLJ010213
3121
474
88.5
globlastp


892
LNU7
tomato|09v1|BG126482
3122
474
88.5
globlastp


892
LNU27
tomato|09v1|BG126482
3122
489
80
globlastp


893
LNU7
citrus|gb166|BQ624128
3123
474
88.5
globlastp


894
LNU7
ipomoea|gb157.2|BJ567189
3120
474
88.5
globlastp


895
LNU7
lettuce|gb157.2|DW044205
3124
474
88.5
globlastp


896
LNU7
lettuce|10v1|DW074561
3125
474
88.5
globlastp


897
LNU7
lettuce|gb157.2|DW074561
3125
474
88.5
globlastp


898
LNU7
lettuce|gb157.2|DW147214
3126
474
88.5
globlastp


899
LNU7
lovegrass|gb167|EH188904
3127
474
88.5
globlastp


899
LNU27
lovegrass|gb167|EH188904
3127
489
86.7
globlastp


900
LNU7
melon|gb165|AM723109
3128
474
88.5
globlastp


901
LNU7
melon|gb165|EB715608
3129
474
88.5
globlastp


902
LNU7
oak|gb170|DN950298
3130
474
88.5
globlastp


903
LNU7
potato|gb157.2|BQ047073
3122
474
88.5
globlastp


903
LNU27
potato|gb157.2|BQ047073
3122
489
80
globlastp


904
LNU7
sunflower|gb162|CD849282
3131
474
88.5
globlastp


905
LNU7
thellungiella|gb167|BI698609
3132
474
88.5
globlastp


906
LNU7
lettuce|10v1|DW044205
3124
474
88.5
globlastp


907
LNU7
arabidopsis_lyrata|09v1|JGIAL009087
3133
474
86.9
globlastp


908
LNU7
arabidopsis_lyrata|09v1|JGIAL013834
3134
474
86.9
globlastp


909
LNU7
canola|10v1|CD816913
3135
474
86.9
globlastp


910
LNU7
canola|10v1|CD838406
3135
474
86.9
globlastp


911
LNU7
canola|10v1|CN732166
3135
474
86.9
globlastp


912
LNU7
gerbera|09v1|AJ754854
3136
474
86.9
globlastp


913
LNU7
ginseng|10v1|AB042860
3137
474
86.9
globlastp


914
LNU7
millet|09v1|CD724720
3138
474
86.9
globlastp


914
LNU27
millet|09v1|CD724720
3138
489
85
globlastp


915
LNU7
salvia|10v1|SRR014553S0006508
3139
474
86.9
globlastp


916
LNU7
solanum_phureja|09v1|SPHBG126482
3140
474
86.9
globlastp


917
LNU7
sorghum|gb161.crp|AI664904
3141
474
86.9
globlastp


917
LNU27
sorghum|gb161.crp|AI664904
3141
489
85
globlastp


918
LNU7
arabidopsis|gb165|AT3G06680
3142
474
86.9
globlastp


919
LNU7
b_juncea|gb164|EVGN00263811010987
3135
474
86.9
globlastp


920
LNU7
b_juncea|gb164|EVGN00818312581324
3135
474
86.9
globlastp


921
LNU7
b_juncea|gb164|EVGN01551409763163
3135
474
86.9
globlastp


922
LNU7
b_juncea|gb164|EVGN01699912462188
3135
474
86.9
globlastp


923
LNU7
b_juncea|gb164|EVGN04088908911493
3143
474
86.9
globlastp


924
LNU7
b_oleracea|gb161|DY027316
3135
474
86.9
globlastp


925
LNU7
b_rapa|gb162|CX267004
3135
474
86.9
globlastp


926
LNU7
b_rapa|gb162|CX268468
3135
474
86.9
globlastp


927
LNU7
beet|gb162|BI096143
3144
474
86.9
globlastp


928
LNU7
canola|10v1|CD811781
3135
474
86.9
globlastp


929
LNU7
canola|gb161|CD811781
3135
474
86.9
globlastp


930
LNU7
canola|10v1|CD812329
3135
474
86.9
globlastp


931
LNU7
canola|gb161|CD812329
3135
474
86.9
globlastp


932
LNU7
canola|gb161|CD816913
3135
474
86.9
globlastp


933
LNU7
canola|gb161|CN732166
3135
474
86.9
globlastp


934
LNU7
canola|10v1|CX278337
3135
474
86.9
globlastp


935
LNU7
canola|gb161|CX278337
3135
474
86.9
globlastp


936
LNU7
cenchrus|gb166|EB653562
3138
474
86.9
globlastp


936
LNU27
cenchrus|gb166|EB653562
3138
489
85
globlastp


937
LNU7
coffea|10v1|DV679308
3145
474
86.9
globlastp


938
LNU7
coffea|gb157.2|DV679308
3145
474
86.9
globlastp


939
LNU7
dandelion|gb161|DY802911
3146
474
86.9
globlastp


940
LNU7
maize|gb170|AI941993
3147
474
86.9
globlastp


940
LNU27
maize|gb170|AI941993
3147
489
83.3
globlastp


941
LNU7
pepper|gb157.2|BM067951
3148
474
86.9
globlastp


941
LNU27
pepper|gb157.2|BM067951
3148
489
80
globlastp


942
LNU7
pepper|gb171|BM067951
3149
474
86.9
globlastp


942
LNU27
pepper|gb171|BM067951
3149
489
80
globlastp


943
LNU7
petunia|gb166|EB174600
3150
474
86.9
globlastp


944
LNU7
petunia|gb171|EB174600
3150
474
86.9
globlastp


945
LNU7
potato|gb157.2|BQ047370
3140
474
86.9
globlastp


946
LNU7
prunus|gb167|BI203148
3151
474
86.9
globlastp


947
LNU7
radish|gb164|EV527807
3134
474
86.9
globlastp


948
LNU7
radish|gb164|EV539631
3152
474
86.9
globlastp


949
LNU7
radish|gb164|EW732099
3135
474
86.9
globlastp


950
LNU7
radish|gb164|EW734121
3135
474
86.9
globlastp


951
LNU7
sorghum|09v1|SB09G017140
3141
474
86.9
globlastp


951
LNU27
sorghum|09v1|SB09G017140
3141
489
85
globlastp


952
LNU7
sorghum|09v1|SB09G017150
3153
474
86.9
globlastp


952
LNU27
sorghum|09v1|SB09G017150
3153
489
85
globlastp


953
LNU7
sorghum|gb161.crp|AW287208
3153
474
86.9
globlastp


953
LNU27
sorghum|gb161.crp|AW287208
3153
489
85
globlastp


954
LNU7
spurge|gb161|DV124618
3154
474
86.9
globlastp


955
LNU7
sugarcane|10v1|BQ530802
3141
474
86.9
globlastp


955
LNU27
sugarcane|10v1|BQ530802
3141
489
85
globlastp


956
LNU7
sugarcane|gb157.3|BQ530802
3141
474
86.9
globlastp


956
LNU27
sugarcane|gb157.3|BQ530802
3141
489
85
globlastp


957
LNU7
sugarcane|10v1|CA118622
3141
474
86.9
globlastp


957
LNU27
sugarcane|10v1|CA118622
3141
489
85
globlastp


958
LNU7
sugarcane|gb157.3|CA118622
3141
474
86.9
globlastp


958
LNU27
sugarcane|gb157.3|CA11862
3141
489
85
globlastp


959
LNU7
switchgrass|gb167|DN150845
3138
474
86.9
globlastp


959
LNU27
switchgrass|gb167|DN150845
3138
489
85
globlastp


960
LNU7
wheat|gb164|CD491023
3141
474
86.9
globlastp


960
LNU27
wheat|gb164|CD491023
3141
489
85
globlastp


961
LNU7
potato|10v1|BQ047073
3140
474
86.9
globlastp


962
LNU7
banana|gb167|DN239847
3155
474
85.5
globlastp


963
LNU7
banana|gb167|FL658741
3156
474
85.5
globlastp


964
LNU7
oil_palm|gb166|EL683904
3157
474
85.5
globlastp


965
LNU7
canola|10v1|BQ704618
3158
474
85.25
glotblastn


966
LNU7
dandelion|gb161|DY814075
3159
474
85.25
glotblastn


967
LNU7
b_nigra|09v1|GT069298
—
474
85.25
glotblastn


968
LNU7
petunia|gb171|CV300233
—
474
85.25
glotblastn


969
LNU7
thellungiella|gb167|BQ087680
—
474
85.25
glotblastn


970
LNU7
basilicum|10v1|DY323081
3160
474
85.2
globlastp


970
LNU27
basilicum|10v1|DY323081
3160
489
80.3
globlastp


971
LNU7
canola|10v1|CD811649
3161
474
85.2
globlastp


972
LNU7
cucumber|09v1|CK700790
3162
474
85.2
globlastp


973
LNU7
gerbera|09v1|AJ762109
3163
474
85.2
globlastp


974
LNU7
lotus|09v1|CRPLJ015426
3164
474
85.2
globlastp


975
LNU7
lotus|09v1|CRPLJ021029
3164
474
85.2
globlastp


976
LNU7
lotus|09v1|CRPLJ033772
3164
474
85.2
globlastp


977
LNU7
brachypodium|09v1|GT762410
3165
474
85.2
globlastp


977
LNU27
brachypodium|09v1|GT762410
3165
489
90
globlastp


978
LNU7
brachypodium|gb169|BE420561
3165
474
85.2
globlastp


978
LNU27
brachypodium|gb169|BE420561
3165
489
90
globlastp


979
LNU7
antirrhinum|gb166|AJ559707
3166
474
85.2
globlastp


980
LNU7
apple|gb157.3|CN444191
3167
474
85.2
globlastp


981
LNU7
apple|gb171|CN444191
3167
474
85.2
globlastp


982
LNU7
apple|gb157.3|CN489474
3167
474
85.2
globlastp


983
LNU7
apple|gb171|CN489474
3167
474
85.2
globlastp


984
LNU7
arabidopsis|gb165|AT3G06700
3168
474
85.2
globlastp


985
LNU7
artemisia|gb164|EY054666
3169
474
85.2
globlastp


986
LNU7
b_juncea|gb164|EVGN00375713871037P0
3170
474
85.2
globlastp


987
LNU7
b_juncea|gb164|EVGN01049614682128
3161
474
85.2
globlastp


988
LNU7
b_rapa|gb162|CV432967
3161
474
85.2
globlastp


989
LNU7
basilicum|gb157.3|DY323081
3160
474
85.2
globlastp


989
LNU27
basilicum|gb157.3|DY323081
3160
489
80.3
globlastp


990
LNU7
beech|gb170|SRR006293S0003253
3171
474
85.2
globlastp


991
LNU7
maize|gb170|AI600790
3172
474
85.2
globlastp


991
LNU27
maize|gb170|AI600790
3172
489
83.3
globlastp


992
LNU7
maize|gb170|AI833392
3173
474
85.2
globlastp


992
LNU27
maize|gb170|AI833392
3173
489
83.3
globlastp


993
LNU7
poplar|10v1|DT492219
3174
474
85.2
globlastp


994
LNU7
poplar|gb170|DT492219
3174
474
85.2
globlastp


995
LNU7
radish|gb164|EV536346
3170
474
85.2
globlastp


996
LNU7
radish|gb164|EV549950
3170
474
85.2
globlastp


997
LNU7
radish|gb164|EW714409
3170
474
85.2
globlastp


998
LNU7
radish|gb164|EX746273
3170
474
85.2
globlastp


999
LNU7
radish|gb164|FD556726
3170
474
85.2
globlastp


1000
LNU7
sunflower|gb162|CD846243
3175
474
85.2
globlastp


1001
LNU7
switchgrass|gb167|DN143529
3176
474
85.2
globlastp


1001
LNU27
switchgrass|gb167|DN143529
3176
489
83.3
globlastp


1002
LNU7
switchgrass|gb167|FL789549
3177
474
85.2
globlastp


1002
LNU27
switchgrass|gb167|FL789549
3177
489
83.3
globlastp


1003
LNU7
tamarix|gb166|CF198845
3178
474
85.2
globlastp


1004
LNU7
avocado|10v1|CK758909
3179
474
83.9
globlastp


1005
LNU7
avocado|gb164|CK758909
3179
474
83.9
globlastp


1006
LNU7
banana|gb167|FF559899
3180
474
83.9
globlastp


1007
LNU7
banana|gb167|FL661163
3181
474
83.9
globlastp


1008
LNU7
lovegrass|gb167|EH190358
3182
474
83.61
glotblastn


1008
LNU27
lovegrass|gb167|EH190358
3182
489
83.33
glotblastn


1009
LNU7
canola|10v1|DW998335
3183
474
83.6
globlastp


1010
LNU7
eggplant|10v1|FS009243
3184
474
83.6
globlastp


1011
LNU7
lettuce|10v1|DW101911
3185
474
83.6
globlastp


1012
LNU7
orobanche|10v1|SRR023189S0004367
3186
474
83.6
globlastp


1012
LNU27
orobanche|10v1|SRR023189S0004367
3186
489
80.3
globlastp


1013
LNU7
brachypodium|09v1|GT764657
3187
474
83.6
globlastp


1013
LNU27
brachypodium|09v1|GT764657
3187
489
88.3
globlastp


1014
LNU7
brachypodium|gb169|BE399643
3187
474
83.6
globlastp


1014
LNU27
brachypodium|gb169|BE399643
3187
489
88.3
globlastp


1015
LNU7
b_juncea|gb164|EVGN00222912251720
3188
474
83.6
globlastp


1016
LNU7
b_juncea|gb164|EVGN00516938790398
3189
474
83.6
globlastp


1017
LNU7
canola|10v1|CD839275
3190
474
83.6
globlastp


1018
LNU7
canola|gb161|CD811649
3190
474
83.6
globlastp


1019
LNU7
canola|gb161|H74817
3183
474
83.6
globlastp


1020
LNU7
lettuce|gb157.2|DW045025
3191
474
83.6
globlastp


1021
LNU7
lettuce|10v1|DW077777
3191
474
83.6
globlastp


1022
LNU7
lettuce|gb157.2|DW077777
3191
474
83.6
globlastp


1023
LNU7
lettuce|gb157.2|DW077988
3191
474
83.6
globlastp


1024
LNU7
lettuce|gb157.2|DW104130
3191
474
83.6
globlastp


1025
LNU7
maize|gb170|AI372387
3192
474
83.6
globlastp


1025
LNU27
maize|gb170|AI372387
3192
489
81.7
globlastp


1026
LNU7
poppy|gb166|FE964149
3193
474
83.6
globlastp


1027
LNU7
triphysaria|gb164|EX992128
3194
474
83.6
globlastp


1027
LNU27
triphysaria|gb164|EX992128
3194
489
83.3
globlastp


1028
LNU7
lettuce|10v1|DW045025
3191
474
83.6
globlastp


1029
LNU7
orobanche|10v1|SRR023495S0017698
3195
474
82.3
globlastp


1030
LNU7
tobacco|gb162|CV020926
3196
474
82.3
globlastp


1031
LNU7
liriodendron|gb166|CK757037
3197
474
82.3
globlastp


1032
LNU7
tobacco|gb162|BU673934
3195
474
82.3
globlastp


1033
LNU7
arabidopsis_lyrata|09v1|JGIAL017844
3198
474
82
globlastp


1034
LNU7
flax|09v1|EU829933
3199
474
82
globlastp


1035
LNU7
monkeyflower|10v1|DV206864
3200
474
82
globlastp


1036
LNU7
oat|10v1|GO582693
3201
474
82
globlastp


1036
LNU27
oat|10v1|GO582693
3201
489
93.3
globlastp


1037
LNU7
oat|10v1|GO582779
3201
474
82
globlastp


1037
LNU27
oat|10v1|GO582779
3201
489
93.3
globlastp


1038
LNU7
orobanche|10v1|SRR023189S0006077
3202
474
82
globlastp


1038
LNU27
orobanche|10v1|SRR023189S0006077
3202
489
80.3
globlastp


1039
LNU7
b_juncea|gb164|EVGN04206719550893
3203
474
82
globlastp


1040
LNU7
cacao|gb167|CU480546
3204
474
82
globlastp


1041
LNU7
dandelion|gb161|DY808273
3205
474
82
globlastp


1042
LNU7
dandelion|gb161|DY811268
3205
474
82
globlastp


1043
LNU7
dandelion|gb161|DY814721
3205
474
82
globlastp


1044
LNU7
lettuce|gb157.2|DW101911
3206
474
82
globlastp


1045
LNU7
rose|10v1|BQ105463
3207
474
82
globlastp


1046
LNU7
rose|gb157.2|BQ105463
3207
474
82
globlastp


1047
LNU7
sunflower|gb162|DY905617
3205
474
82
globlastp


1048
LNU7
switchgrass|gb167|DN150598
3208
474
82
globlastp


1048
LNU27
switchgrass|gb167|DN150598
3208
489
81.7
globlastp


1049
LNU7
cichorium|gb171|FL680147
3209
474
81.97
glotblastn


1050
LNU7
cycas|gb166|CB093385
3210
474
81.5
globlastp


1051
LNU7
strawberry|gb164|CO380923
3211
474
81
globlastp


1052
LNU7
tobacco|gb162|CV019192
3212
474
80.6
globlastp


1053
LNU7
ipomoea_batatas|10v1|DV037499XX2
3213
474
80.33
glotblastn


1054
LNU7
lotus|09v1|BW596153
3214
474
80.33
glotblastn


1055
LNU7
lotus|gb157.2|BP059519
3215
474
80.33
glotblastn


1056
LNU7
monkeyflower|10v1|CV521685
3216
474
80.3
globlastp


1057
LNU7
solanum_phureja|09v1|SPHAF204786
3217
474
80.3
globlastp


1058
LNU7
potato|10v1|BQ512966
3217
474
80.3
globlastp


1059
LNU7
potato|gb157.2|BQ512966
3217
474
80.3
globlastp


1060
LNU7
tobacco|gb162|BP530058
3218
474
80.3
globlastp


1061
LNU7
tomato|09v1|AF204786
3219
474
80.3
globlastp


1062
LNU7
tomato|gb164|AF204786
3219
474
80.3
globlastp


1063
LNU7
cryptomeria|gb166|BW992620
3220
474
80
globlastp


1064
LNU8
arabidopsis_lyrata|09v1|JGIAL010354
3221
475
93.6
globlastp


1065
LNU9
rice|gb170|OS07G37280
3222
476
87.84
glotblastn


1066
LNU13
sorghum|09v1|SB02G036230
3223
480
81.8
globlastp


1067
LNU13
sorghum|gb161.crp|BQ635805
3223
480
81.8
globlastp


1068
LNU13
maize|gb170|BI245385
3224
480
80.7
globlastp


1069
LNU14
arabidopsis_lyrata|09v1|JGIAL015001
3225
481
96.3
globlastp


1070
LNU15
arabidopsis_lyrata|09v1|JGIAL009168
3226
482
94.2
globlastp


1071
LNU17
sorghum|09v1|SB03G011640
3227
483
84.6
globlastp


1072
LNU17
sorghum|gb161.crp|AI947401
3227
483
84.6
globlastp


1073
LNU17
millet|09v1|EVO454PM011107
3228
483
84.1
globlastp


1074
LNU17
sugarcane|gb157.3|CA114497
3229
483
84.1
globlastp


1075
LNU17
switchgrass|gb167|DN142702
3230
483
82.5
globlastp


1076
LNU17
maize|gb170|AI861546
3231
483
81.2
globlastp


1077
LNU17
brachypodium|09v1|GT758222
3232
483
80.5
globlastp


1078
LNU17
brachypodium|gb169|BQ246612
3232
483
80.5
globlastp


1079
LNU19
maize|gb170|DR806345
3233
484
82.6
globlastp


1080
LNU19
sorghum|gb161.crp|AW679176
3234
484
80.5
globlastp


1081
LNU20
potato|gb157.2|BG888517
3235
485
97.8
globlastp


1082
LNU20
potato|10v1|BG888517
3236
485
97.6
globlastp


1083
LNU20
solanum_phureja|09v1|SPHBG131270
3237
485
97.4
globlastp


1084
LNU20
pepper|gb171|BM062238
3238
485
89.5
globlastp


1085
LNU20
tobacco|gb162|EB428440
3239
485
83.74
glotblastn


1086
LNU23
arabidopsis_lyrata|09v1|JGIAL002476
3240
486
93.2
globlastp


1087
LNU23
radish|gb164|EW732145
3241
486
88.5
globlastp


1088
LNU24
arabidopsis_lyrata|09v1|JGIAL003443
3242
487
98.2
globlastp


1089
LNU24
radish|gb164|EV528988
3243
487
87
globlastp


1090
LNU24
arabidopsis|gb165|AT1G33090
3244
487
85.8
globlastp


1091
LNU24
arabidopsis|gb165|AT1G33100
3245
487
85.2
globlastp


1092
LNU24
arabidopsis_lyrata|09v1|JGIAL003442
3246
487
85
globlastp


1093
LNU24
arabidopsis|gb165|AT1G33080
3247
487
83.2
globlastp


1094
LNU25
sugarcane|10v1|BQ533886
3248
488
96.1
globlastp


1095
LNU25
sugarcane|gb157.3|BQ533886
3249
488
96.1
globlastp


1096
LNU25
maize|gb170|AW563076
3250
488
93.6
globlastp


1097
LNU25
switchgrass|gb167|FL773555
3251
488
83.3
globlastp


1098
LNU27
wheat|gb164|BE399643
489
489
100
globlastp


1099
LNU27
wheat|gb164|BE424751
489
489
100
globlastp


1100
LNU27
wheat|gb164|BE443944
489
489
100
globlastp


1101
LNU27
rye|gb164|BG263912
3252
489
96.7
globlastp


1102
LNU27
fescue|gb161|CK803089
3253
489
85
globlastp


1103
LNU28
wheat|gb164|BF293133
3254
490
97.1
globlastp


1104
LNU28
pseudoroegneria|gb167|FF346547
3255
490
96
globlastp


1105
LNU28
wheat|gb164|CA655539
3256
490
95.7
globlastp


1106
LNU28
leymus|gb166|EG382149
3257
490
95.5
globlastp


1107
LNU28
brachypodium|09v1|GT829440
3258
490
85.2
globlastp


1108
LNU28
brachypodium|gb169|BF293133
3258
490
85.2
globlastp


1109
LNU29
solanum_phureja|09v1|SPHAI487919
3259
491
91.2
globlastp


1110
LNU29
potato|gb157.2|BM405532
3260
491
88.33
glotblastn


1111
LNU32
sugarcane|10v1|CA070626
3261
492
91.12
glotblastn


1112
LNU32
sugarcane|gb157.3|CA070626
3262
492
87
globlastp


1113
LNU32
sorghum|09v1|SB08G001710
3263
492
85.1
globlastp


1114
LNU32
sorghum|gb161.crp|CD463367
3263
492
85.1
globlastp


1115
LNU32
maize|gb170|CB604763
3264
492
84.8
globlastp


1116
LNU32
maize|gb170|BE552794
3265
492
83.3
globlastp


1117
LNU32
switchgrass|gb167|DN144499
3266
492
82.6
globlastp


1118
LNU33
soybean|gb168|BQ124735
3267
493
92.95
glotblastn


1119
LNU33
lotus|09v1|AV776761
3268
493
80.6
globlastp


1120
LNU34
sorghum|09v1|SB03G034160
3269
494
87.86
glotblastn


1121
LNU34
sorghum|gb161.crp|DN212069
3270
494
87.86
glotblastn


1122
LNU34
brachypodium|09v1|GT773303
3271
494
86.43
glotblastn


1123
LNU34
wheat|gb164|BG608344
3272
494
85.71
glotblastn


1124
LNU34
maize|gb170|BE344718
3273
494
85.2
globlastp


1125
LNU36
soybean|gb168|BE823007
3274
496
94.3
globlastp


1126
LNU36
soybean|gb168|CD398253
3275
496
80.7
globlastp


1127
LNU43
soybean|gb168|AI967672
3276
499
94.9
globlastp


1128
LNU43
bean|gb167|CA896732
3277
499
90.4
globlastp


1129
LNU43
liquorice|gb171|FS261351
3278
499
89.9
globlastp


1130
LNU43
cowpea|gb166|FF399439
3279
499
89.3
globlastp


1131
LNU43
peanut|gb171|ES721626
3280
499
88
globlastp


1132
LNU43
peanut|gb167|EE125486
3281
499
87
globlastp


1133
LNU43
peanut|gb171|EE125486
3281
499
87
globlastp


1134
LNU43
lotus|09v1|LLAI967672
3282
499
84.9
globlastp


1135
LNU43
lotus|gb157.2|AI967672
3282
499
84.9
globlastp


1136
LNU43
chickpea|09v2|FE669917
3283
499
84.8
globlastp


1137
LNU43
pea|09v1|GFXPEAATPASEX1
3284
499
81.3
globlastp


1138
LNU44
pigeonpea|gb171|GR464245
3285
500
94.9
globlastp


1139
LNU44
cowpea|gb166|FC459300
3286
500
92.4
globlastp


1140
LNU44
liquorice|gb171|FS238932
3287
500
89.9
globlastp


1141
LNU44
bean|gb167|CB539787
3288
500
87.34
glotblastn


1142
LNU44
bean|gb167|CA899920
3289
500
86.1
globlastp


1143
LNU44
lotus|09v1|LLCN825274
3290
500
86.1
globlastp


1144
LNU44
lotus|gb157.2|CN825274
3290
500
86.1
globlastp


1145
LNU44
soybean|gb168|BQ155489
3291
500
84.7
globlastp


1146
LNU44
bean|gb167|FD799417
3292
500
83.5
globlastp


1147
LNU44
medicago|09v1|AW171675
3293
500
83.5
globlastp


1148
LNU44
medicago|gb157.2|AW171675
3293
500
83.5
globlastp


1149
LNU44
peanut|gb167|CD038813
3294
500
81.2
globlastp


1150
LNU44
peanut|gb171|CD038813
3294
500
81.2
globlastp


1151
LNU44
peanut|gb171|CD038024
3295
500
80
globlastp


1152
LNU45
chickpea|09v2|GR406612
501
501
100
globlastp


1153
LNU45
liquorice|gb171|FS238653
501
501
100
globlastp


1154
LNU45
pea|09v1|AM161941
501
501
100
globlastp


1155
LNU45
pigeonpea|gb171|GR465032
501
501
100
globlastp


1156
LNU45
bean|gb167|CA897298
501
501
100
globlastp


1157
LNU45
chestnut|gb170|SRR006295S0059092
501
501
100
globlastp


1158
LNU45
cowpea|gb166|DR068382
501
501
100
globlastp


1159
LNU45
cowpea|gb166|EG594283
501
501
100
globlastp


1160
LNU45
cowpea|gb166|FC456876
501
501
100
globlastp


1161
LNU45
lotus|09v1|BI419054
501
501
100
globlastp


1162
LNU45
lotus|gb157.2|BI419054
501
501
100
globlastp


1163
LNU45
lotus|09v1|LLCB829590
501
501
100
globlastp


1164
LNU45
lotus|gb157.2|CB829590
501
501
100
globlastp


1165
LNU45
medicago|09v1|BE318806
501
501
100
globlastp


1166
LNU45
medicago|gb157.2|BE318806
501
501
100
globlastp


1167
LNU45
oak|gb170|CR627523
501
501
100
globlastp


1168
LNU45
peanut|gb167|CD037890
501
501
100
globlastp


1169
LNU45
peanut|gb171|CD037890
501
501
100
globlastp


1170
LNU45
peanut|gb171|CD038469
501
501
100
globlastp


1171
LNU45
peanut|gb167|EE126116
501
501
100
globlastp


1172
LNU45
peanut|gb171|EE126116
501
501
100
globlastp


1173
LNU45
peanut|gb167|EE126336
501
501
100
globlastp


1174
LNU45
peanut|gb171|EE126336
501
501
100
globlastp


1175
LNU45
chickpea|09v2|GR392190
3296
501
98.8
globlastp


1176
LNU45
chickpea|09v2|GR392639
3297
501
98.8
globlastp


1177
LNU45
cleome_gynandra|10v1|SRR015532S0009070
3296
501
98.8
globlastp


1178
LNU45
cucumber|09v1|CK086106
3298
501
98.8
globlastp


1179
LNU45
heritiera|10v1|SRR005795S0009553
3299
501
98.8
globlastp


1180
LNU45
heritiera|10v1|SRR005795S0022077
3299
501
98.8
globlastp


1181
LNU45
liquorice|gb171|FS245788
3300
501
98.8
globlastp


1182
LNU45
bean|gb167|CA897297
3300
501
98.8
globlastp


1183
LNU45
beech|gb170|SRR006293S0000924
3298
501
98.8
globlastp


1184
LNU45
cacao|gb167|CU473827
3299
501
98.8
globlastp


1185
LNU45
cassava|gb164|DV442696
3298
501
98.8
globlastp


1186
LNU45
castorbean|09v1|EE256323
3298
501
98.8
globlastp


1187
LNU45
castorbean|gb160|EE256323
3298
501
98.8
globlastp


1188
LNU45
castorbean|09v1|GE636711
3298
501
98.8
globlastp


1189
LNU45
chestnut|gb170|SRR006295S0001785
3298
501
98.8
globlastp


1190
LNU45
cotton|gb164|BE052927
3299
501
98.8
globlastp


1191
LNU45
cotton|gb164|BE053779
3299
501
98.8
globlastp


1192
LNU45
cotton|gb164|BE054840
3299
501
98.8
globlastp


1193
LNU45
cotton|gb164|BF275747
3299
501
98.8
globlastp


1194
LNU45
cotton|gb164|BG444626
3299
501
98.8
globlastp


1195
LNU45
cotton|gb164|CO104281
3299
501
98.8
globlastp


1196
LNU45
cowpea|gb166|FC460219
3300
501
98.8
globlastp


1197
LNU45
eucalyptus|gb166|CB967805
3298
501
98.8
globlastp


1198
LNU45
eucalyptus|gb166|CT980235
3298
501
98.8
globlastp


1199
LNU45
medicago|gb1579.2|AW329579
3296
501
98.8
globlastp


1200
LNU45
medicago|09v1|LLBE239494
3296
501
98.8
globlastp


1201
LNU45
medicago|gb157.2|BE239494
3296
501
98.8
globlastp


1202
LNU45
melon|gb165|EB714819
3298
501
98.8
globlastp


1203
LNU45
oak|gb170|DN950003
3298
501
98.8
globlastp


1204
LNU45
peanut|gb167|EH046888
3301
501
98.8
globlastp


1205
LNU45
peanut|gb171|EH046888
3301
501
98.8
globlastp


1206
LNU45
rose|10v1|BQ104562
3302
501
98.8
globlastp


1207
LNU45
soybean|gb168|BM140026
3300
501
98.8
globlastp


1208
LNU45
spurge|gb161|BE095304
3298
501
98.8
globlastp


1209
LNU45
cleome_spinosa|10v1|GR931938
3303
501
97.7
globlastp


1210
LNU45
cleome_spinosa|10v1|SRR015531S0016648
3303
501
97.7
globlastp


1211
LNU45
cleome_spinosa|10v1|SRR015531S0024494
3303
501
97.7
globlastp


1212
LNU45
cleome_spinosa|10v1|SRR015531S0039098
3303
501
97.7
globlastp


1213
LNU45
cucumber|09v1|AM715462
3304
501
97.7
globlastp


1214
LNU45
lettuce|10v1|DW075415
3305
501
97.7
globlastp


1215
LNU45
liquorice|gb171|FS239649
3306
501
97.7
globlastp


1216
LNU45
monkeyflower|10v1|CV519036
3307
501
97.7
globlastp


1217
LNU45
pea|09v1|EX570516
3308
501
97.7
globlastp


1218
LNU45
pea|09v1|EX571249
3309
501
97.7
globlastp


1219
LNU45
tea|10v1|CV013950
3310
501
97.7
globlastp


1220
LNU45
antirrhinum|gb166|AJ558887
3311
501
97.7
globlastp


1221
LNU45
beet|gb162|BQ592037
3312
501
97.7
globlastp


1222
LNU45
bruguiera|gb166|BP941557
3313
501
97.7
globlastp


1223
LNU45
cacao|gb167|CF974299
3314
501
97.7
globlastp


1224
LNU45
cacao|gb167|CU476326
3315
501
97.7
globlastp


1225
LNU45
cassava|09v1|BI325193
3316
501
97.7
globlastp


1226
LNU45
cassava|gb164|BI325193
3316
501
97.7
globlastp


1227
LNU45
cassava|09v1|CK644610
3317
501
97.7
globlastp


1228
LNU45
cassava|gb164|CK644610
3317
501
97.7
globlastp


1229
LNU45
cassava|09v1|DV442696
3317
501
97.7
globlastp


1230
LNU45
castorbean|gb160|MDL30128M008573
3318
501
97.7
globlastp


1231
LNU45
cycas|gb166|CB091386
3319
501
97.7
globlastp


1232
LNU45
cycas|gb166|CB092866
3319
501
97.7
globlastp


1233
LNU45
grape|gb160|EC932417
3320
501
97.7
globlastp


1234
LNU45
iceplant|gb164|BE034168
3321
501
97.7
globlastp


1235
LNU45
lettuce|gb157.2|DW075415
3305
501
97.7
globlastp


1236
LNU45
lettuce|gb157.2|DW103341
3305
501
97.7
globlastp


1237
LNU45
lettuce|gb157.2|DW145378
3305
501
97.7
globlastp


1238
LNU45
prunus|gb167|CB821790
3322
501
97.7
globlastp


1239
LNU45
rose|gb157.2|BQ104562
3323
501
97.7
globlastp


1240
LNU45
spurge|gb161|DV133006
3324
501
97.7
globlastp


1241
LNU45
strawberry|gb164|CO379162
3325
501
97.7
globlastp


1242
LNU45
tamarix|gb166|CN605485
3312
501
97.7
globlastp


1243
LNU45
walnuts|gb166|CV197870
3312
501
97.7
globlastp


1244
LNU45
zamia|gb166|DY034316
3319
501
97.7
globlastp


1245
LNU45
canola|10v1|CD817525
3326
501
96.51
glotblastn


1246
LNU45
blueberry|10v1|CF811404
3327
501
96.5
globlastp


1247
LNU45
canola|10v1|CD839015
3328
501
96.5
globlastp


1248
LNU45
canola|10v1|CN731675
3328
501
96.5
globlastp


1249
LNU45
canola|10v1|EE476478
3328
501
96.5
globlastp


1250
LNU45
cleome_gynandra|10v1|SRR015532S0012040
3329
501
96.5
globlastp


1251
LNU45
flax|09v1|EU829812
3330
501
96.5
globlastp


1252
LNU45
gerbera|09v1|AJ761450
3331
501
96.5
globlastp


1253
LNU45
ipomoea_nil|10v1|BJ554792
3332
501
96.5
globlastp


1254
LNU45
ipomoea_nil|10v1|BJ559906
3332
501
96.5
globlastp


1255
LNU45
ipomoea_nil|10v1|CJ742456
3332
501
96.5
globlastp


1256
LNU45
monkeyflower|10v1|DV212640
3333
501
96.5
globlastp


1257
LNU45
salvia|10v1|CV164158
3333
501
96.5
globlastp


1258
LNU45
salvia|10v1|CV165453
3333
501
96.5
globlastp


1259
LNU45
amborella|gb166|FD437556
3334
501
96.5
globlastp


1260
LNU45
antirrhinum|gb166|AJ560227
3335
501
96.5
globlastp


1261
LNU45
apple|gb157.3|CN492050
3336
501
96.5
globlastp


1262
LNU45
apple|gb171|CN492050
3336
501
96.5
globlastp


1263
LNU45
apple|gb157.3|CN997325
3336
501
96.5
globlastp


1264
LNU45
apple|gb171|CN997325
3336
501
96.5
globlastp


1265
LNU45
b_juncea|gb164|EVGN00032311610584
3328
501
96.5
globlastp


1266
LNU45
b_juncea|gb164|EVGN00163218130726
3328
501
96.5
globlastp


1267
LNU45
b_juncea|gb164|EVGN00242617670457
3328
501
96.5
globlastp


1268
LNU45
b_juncea|gb164|EVGN00404524182700
3328
501
96.5
globlastp


1269
LNU45
b_juncea|gb164|EVGN00541511341883
3328
501
96.5
globlastp


1270
LNU45
b_juncea|gb164|EVGN00673809061646
3328
501
96.5
globlastp


1271
LNU45
b_juncea|gb164|EVGN00683412381058
3328
501
96.5
globlastp


1272
LNU45
b_juncea|gb164|EVGN01161211992680
3328
501
96.5
globlastp


1273
LNU45
b_juncea|gb164|EVGN01304909632819
3328
501
96.5
globlastp


1274
LNU45
b_juncea|gb164|EVGN04290618070322
3328
501
96.5
globlastp


1275
LNU45
b_oleracea|gb161|AM062107
3328
501
96.5
globlastp


1276
LNU45
b_oleracea|gb161|DY027039
3328
501
96.5
globlastp


1277
LNU45
b_oleracea|gb161|DY027348
3328
501
96.5
globlastp


1278
LNU45
b_oleracea|gb161|DY027603
3328
501
96.5
globlastp


1279
LNU45
b_oleracea|gb161|DY029297
3328
501
96.5
globlastp


1280
LNU45
b_rapa|gb162|BG544410
3328
501
96.5
globlastp


1281
LNU45
b_rapa|gb162|CA992030
3328
501
96.5
globlastp


1282
LNU45
b_rapa|gb162|CV432516
3328
501
96.5
globlastp


1283
LNU45
b_rapa|gb162|CV433072
3328
501
96.5
globlastp


1284
LNU45
b_rapa|gb162|CX266536
3328
501
96.5
globlastp


1285
LNU45
b_rapa|gb162|CX268525
3328
501
96.5
globlastp


1286
LNU45
b_rapa|gb162|CX270594
3328
501
96.5
globlastp


1287
LNU45
b_rapa|gb162|CX273157
3328
501
96.5
globlastp


1288
LNU45
b_rapa|gb162|EE530283
3328
501
96.5
globlastp


1289
LNU45
bruguiera|gb166|BP948881
3337
501
96.5
globlastp


1290
LNU45
canola|gb161|CD812378
3328
501
96.5
globlastp


1291
LNU45
canola|gb161|CD812394
3328
501
96.5
globlastp


1292
LNU45
canola|10v1|CD812830
3328
501
96.5
globlastp


1293
LNU45
canola|gb161|CD812830
3328
501
96.5
globlastp


1294
LNU45
canola|gb161|CD812870
3328
501
96.5
globlastp


1295
LNU45
canola|gb161|CD817916
3328
501
96.5
globlastp


1296
LNU45
canola|10v1|CD818245
3328
501
96.5
globlastp


1297
LNU45
canola|gb161|CD818245
3328
501
96.5
globlastp


1298
LNU45
canola|gb161|CD818496
3328
501
96.5
globlastp


1299
LNU45
canola|gb161|CD821364
3328
501
96.5
globlastp


1300
LNU45
canola|gb161|CD834560
3328
501
96.5
globlastp


1301
LNU45
canola|gb161|CN731675
3328
501
96.5
globlastp


1302
LNU45
canola|gb161|EE476478
3328
501
96.5
globlastp


1303
LNU45
centaurea|gb166|EH740133
3338
501
96.5
globlastp


1304
LNU45
centaurea|gb166|EH744958
3338
501
96.5
globlastp


1305
LNU45
centaurea|gb166|EH785564
3331
501
96.5
globlastp


1306
LNU45
citrus|gb166|BQ624315
3339
501
96.5
globlastp


1307
LNU45
citrus|gb166|BQ624832
3339
501
96.5
globlastp


1308
LNU45
clover|gb162|BB930040
3340
501
96.5
globlastp


1309
LNU45
cryptomeria|gb166|BP174475
3341
501
96.5
globlastp


1310
LNU45
cynara|gb167|GE585914
3331
501
96.5
globlastp


1311
LNU45
ginger|gb164|DY349602
3342
501
96.5
globlastp


1312
LNU45
grape|gb160|BE846411
3343
501
96.5
globlastp


1313
LNU45
ipomoea|gb157.2|CJ741047
3332
501
96.5
globlastp


1314
LNU45
ipomoea|gb157.2|CJ742456
3332
501
96.5
globlastp


1315
LNU45
lettuce|10v1|DW044163
3331
501
96.5
globlastp


1316
LNU45
lettuce|gb157.2|DW044163
3331
501
96.5
globlastp


1317
LNU45
lettuce|gb157.2|DW045774
3331
501
96.5
globlastp


1318
LNU45
lettuce|gb157.2|DW103758
3331
501
96.5
globlastp


1319
LNU45
lettuce|gb157.2|DW105810
3331
501
96.5
globlastp


1320
LNU45
melon|gb165|AM715462
3344
501
96.5
globlastp


1321
LNU45
nuphar|gb166|CD474984
3345
501
96.5
globlastp


1322
LNU45
oil_palm|gb166|EL684405
3346
501
96.5
globlastp


1323
LNU45
oil_palm|gb166|EY413173
3342
501
96.5
globlastp


1324
LNU45
papaya|gb165|AM903803
3347
501
96.5
globlastp


1325
LNU45
papaya|gb165|EX239749
3348
501
96.5
globlastp


1326
LNU45
pine|10v1|AI812758
3349
501
96.5
globlastp


1327
LNU45
pine|gb157.2|AI812758
3349
501
96.5
globlastp


1328
LNU45
poplar|10v1|AI165443
3350
501
96.5
globlastp


1329
LNU45
poplar|gb170|AI165443
3350
501
96.5
globlastp


1330
LNU45
poplar|10v1|BI070097
3350
501
96.5
globlastp


1331
LNU45
poplar|gb170|BI070097
3350
501
96.5
globlastp


1332
LNU45
poplar|10v1|BI119656
3350
501
96.5
globlastp


1333
LNU45
poplar|gb170|BI119656
3350
501
96.5
globlastp


1334
LNU45
prunus|gb167|BU039142
3351
501
96.5
globlastp


1335
LNU45
radish|gb164|EV525442
3328
501
96.5
globlastp


1336
LNU45
radish|gb164|EV527675
3328
501
96.5
globlastp


1337
LNU45
radish|gb164|EV536763
3328
501
96.5
globlastp


1338
LNU45
radish|gb164|EV537524
3328
501
96.5
globlastp


1339
LNU45
radish|gb164|EW723868
3328
501
96.5
globlastp


1340
LNU45
radish|gb164|EW725365
3328
501
96.5
globlastp


1341
LNU45
radish|gb164|EW733186
3328
501
96.5
globlastp


1342
LNU45
radish|gb164|EW734391
3328
501
96.5
globlastp


1343
LNU45
radish|gb164|EX757217
3328
501
96.5
globlastp


1344
LNU45
radish|gb164|EX765397
3328
501
96.5
globlastp


1345
LNU45
radish|gb164|EX895252
3328
501
96.5
globlastp


1346
LNU45
radish|gb164|EY905533
3328
501
96.5
globlastp


1347
LNU45
radish|gb164|EY934770
3328
501
96.5
globlastp


1348
LNU45
spruce|gb162|CO227497
3349
501
96.5
globlastp


1349
LNU45
strawberry|gb164|CO379638
3352
501
96.5
globlastp


1350
LNU45
sunflower|gb162|CD849156
3331
501
96.5
globlastp


1351
LNU45
sunflower|gb162|CD849309
3331
501
96.5
globlastp


1352
LNU45
triphysaria|gb164|EX989107
3333
501
96.5
globlastp


1353
LNU45
triphysaria|gb164|EY001721
3333
501
96.5
globlastp


1354
LNU45
zamia|gb166|DY036444
3353
501
96.5
globlastp


1355
LNU45
lettuce|10v1|DW045774
3331
501
96.5
globlastp


1356
LNU45
lettuce|10v1|DW099098
3331
501
96.5
globlastp


1357
LNU45
canola|10v1|CD812870
3328
501
96.5
globlastp


1358
LNU45
canola|10v1|CD812378
3328
501
96.5
globlastp


1359
LNU45
canola|10v1|CD834560
3328
501
96.5
globlastp


1360
LNU45
salvia|10v1|SRR014553S0002286
3354
501
95.35
glotblastn


1361
LNU45
b_juncea|gb164|EVGN00337914530877
3355
501
95.35
glotblastn


1362
LNU45
canola|gb161|EL590902
3356
501
95.35
glotblastn


1363
LNU45
citrus|gb166|CF503931
3357
501
95.35
glotblastn


1364
LNU45
safflower|gb162|EL403359
3358
501
95.35
glotblastn


1365
LNU45
arabidopsis_lyrata|09v1|JGIAL029003
3359
501
95.3
globlastp


1366
LNU45
avocado|10v1|FD506790
3360
501
95.3
globlastp


1367
LNU45
cichorium|gb171|EH702627
3361
501
95.3
globlastp


1368
LNU45
eggplant|10v1|FS000719
3362
501
95.3
globlastp


1369
LNU45
potato|10v1|BG589651
3363
501
95.3
globlastp


1370
LNU45
salvia|10v1|SRR014553S0005980
3364
501
95.3
globlastp


1371
LNU45
apple|gb171|CN490097
3365
501
95.3
globlastp


1372
LNU45
arabidopsis|gb165|AT3G61110
3366
501
95.3
globlastp


1373
LNU45
artemisia|gb164|EY036326
3367
501
95.3
globlastp


1374
LNU45
artemisia|gb164|EY037581
3367
501
95.3
globlastp


1375
LNU45
avocado|10v1|CK754126
3368
501
95.3
globlastp


1376
LNU45
avocado|gb164|CK754126
3368
501
95.3
globlastp


1377
LNU45
banana|gb167|ES435098
3369
501
95.3
globlastp


1378
LNU45
banana|gb167|FF560357
3370
501
95.3
globlastp


1379
LNU45
banana|gb167|FF562322
3371
501
95.3
globlastp


1380
LNU45
canola|10v1|EE455490
3372
501
95.3
globlastp


1381
LNU45
canola|gb161|EE455490
3372
501
95.3
globlastp


1382
LNU45
catharanthus|gb166|EG561174
3373
501
95.3
globlastp


1383
LNU45
catharanthus|gb166|FD415347
3373
501
95.3
globlastp


1384
LNU45
cichorium|gb166|DT213797
3361
501
95.3
globlastp


1385
LNU45
cichorium|gb171|DT213797
3361
501
95.3
globlastp


1386
LNU45
citrus|gb166|CX640799
3374
501
95.3
globlastp


1387
LNU45
cryptomeria|gb166|BP174101
3375
501
95.3
globlastp


1388
LNU45
cynara|gb167|GE585853
3376
501
95.3
globlastp


1389
LNU45
ginger|gb164|DY351710
3377
501
95.3
globlastp


1390
LNU45
ginger|gb164|DY358500
3377
501
95.3
globlastp


1391
LNU45
ginger|gb164|DY367611
3378
501
95.3
globlastp


1392
LNU45
ipomoea|gb157.2|BJ554792
3379
501
95.3
globlastp


1393
LNU45
kiwi|gb166|FG410222
3380
501
95.3
globlastp


1394
LNU45
kiwi|gb166|FG430714
3380
501
95.3
globlastp


1395
LNU45
kiwi|gb166|FG441586
3380
501
95.3
globlastp


1396
LNU45
kiwi|gb166|FG461878
3380
501
95.3
globlastp


1397
LNU45
lettuce|10v1|DW077971
3379
501
95.3
globlastp


1398
LNU45
lettuce|gb157.2|DW077971
3379
501
95.3
globlastp


1399
LNU45
liriodendron|gb166|CO999247
3377
501
95.3
globlastp


1400
LNU45
liriodendron|gb166|FD495039
3377
501
95.3
globlastp


1401
LNU45
nicotiana_benthamiana|gb162|
3367
501
95.3
globlastp




CN744078






1402
LNU45
oil_palm|gb166|EL681535
3381
501
95.3
globlastp


1403
LNU45
oil_palm|gb166|EL684385
3377
501
95.3
globlastp


1404
LNU45
pepper|gb157.2|CA514595
3362
501
95.3
globlastp


1405
LNU45
pepper|gb171|CA514595
3362
501
95.3
globlastp


1406
LNU45
pine|gb157.2|AA739876
3382
501
95.3
globlastp


1407
LNU45
pine|gb157.2|AI812974
3382
501
95.3
globlastp


1408
LNU45
pine|gb157.2|AL749664
3382
501
95.3
globlastp


1409
LNU45
potato|gb157.2|BE923191
3363
501
95.3
globlastp


1410
LNU45
potato|gb157.2|BF153777
3363
501
95.3
globlastp


1411
LNU45
potato|gb157.2|BG589651
3363
501
95.3
globlastp


1412
LNU45
potato|gb157.2|BM406913
3363
501
95.3
globlastp


1413
LNU45
radish|gb164|EV535745
3383
501
95.3
globlastp


1414
LNU45
rose|10v1|BQ106521
3384
501
95.3
globlastp


1415
LNU45
sesame|gb157.2|BU668222
3385
501
95.3
globlastp


1416
LNU45
spruce|gb162|CO217320
3382
501
95.3
globlastp


1417
LNU45
sunflower|gb162|CD849221
3386
501
95.3
globlastp


1418
LNU45
sunflower|gb162|CD851828
3387
501
95.3
globlastp


1419
LNU45
sunflower|gb162|DY954225
3386
501
95.3
globlastp


1420
LNU45
thellungiella|gb167|BM985525
3372
501
95.3
globlastp


1421
LNU45
tobacco|gb162|CV016291
3367
501
95.3
globlastp


1422
LNU45
tobacco|gb162|CV018253
3367
501
95.3
globlastp


1423
LNU45
tomato|gb164|BG124194
3362
501
95.3
globlastp


1424
LNU45
tomato|gb164|BG126885
3362
501
95.3
globlastp


1425
LNU45
tomato|gb164|BG134762
3362
501
95.3
globlastp


1426
LNU45
pine|10v1|AA739876
3382
501
95.3
globlastp


1427
LNU45
potato|10v1|AJ489106
3363
501
95.3
globlastp


1428
LNU45
potato|10v1|BM406913
3363
501
95.3
globlastp


1429
LNU45
tomato|09v1|BG124194
3362
501
95.3
globlastp


1430
LNU45
arabidopsis_lyrata|09v1|BQ834271
3388
501
94.2
globlastp


1431
LNU45
arabidopsis_lyrata|09v1|JGIAL015965
3389
501
94.2
globlastp


1432
LNU45
ipomoea_batatas|10v1|CB330065
3390
501
94.2
globlastp


1433
LNU45
ipomoea_batatas|10v1|CB330743
3391
501
94.2
globlastp


1434
LNU45
ipomoea_batatas|10v1|CO500840
3392
501
94.2
globlastp


1435
LNU45
monkeyflower|10v1|DV211088
3393
501
94.2
globlastp


1436
LNU45
orobanche|10v1|SRR023189S0002696
3394
501
94.2
globlastp


1437
LNU45
orobanche|10v1|SRR023189S0007530
3394
501
94.2
globlastp


1438
LNU45
solanum_phureja|09v1|SPHBG124194
3395
501
94.2
globlastp


1439
LNU45
apple|gb157.3|CO756008
3396
501
94.2
globlastp


1440
LNU45
arabidopsis|gb165|AT5G47930
3397
501
94.2
globlastp


1441
LNU45
banana|gb167|DN239263
3398
501
94.2
globlastp


1442
LNU45
canola|gb161|EE569888
3399
501
94.2
globlastp


1443
LNU45
catharanthus|gb166|EG560431
3400
501
94.2
globlastp


1444
LNU45
coffea|10v1|DV667447
3401
501
94.2
globlastp


1445
LNU45
coffea|gb157.2|DV667447
3401
501
94.2
globlastp


1446
LNU45
cotton|gb164|BF272631
3402
501
94.2
globlastp


1447
LNU45
dandelion|gb161|DY807943
3403
501
94.2
globlastp


1448
LNU45
ipomoea|gb157.2|CB330743
3391
501
94.2
globlastp


1449
LNU45
nicotiana_benthamiana|gb162|
3404
501
94.2
globlastp




AY310774






1450
LNU45
nicotiana_benthamiana|gb162|
3405
501
94.2
globlastp




CN743261






1451
LNU45
potato|gb157.2|AJ489106
3395
501
94.2
globlastp


1452
LNU45
potato|gb157.2|BM404024
3395
501
94.2
globlastp


1453
LNU45
radish|gb164|EY920230
3406
501
94.2
globlastp


1454
LNU45
rose|gb157.2|BQ106521
3407
501
94.2
globlastp


1455
LNU45
senecio|gb170|DY662196
3408
501
94.2
globlastp


1456
LNU45
sesame|10v1|BU670278
3409
501
94.2
globlastp


1457
LNU45
tobacco|gb162|CV016119
3404
501
94.2
globlastp


1458
LNU45
triphysaria|gb164|EY020166
3410
501
94.2
globlastp


1459
LNU45
pepper|gb171|BM066089
3411
501
94.19
glotblastn


1460
LNU45
pepper|gb157.2|BM062650
3412
501
94.19
glotblastn


1461
LNU45
avocado|10v1|FD507705
3413
501
93.02
glotblastn


1462
LNU45
barley|gb157SOLEXA|BE411675
3414
501
93.02
glotblastn


1462
LNU45
barley|gb157.3|BE411675
3417
501
93
globlastp


1463
LNU45
physcomitrella|gb157|AW127011
3415
501
93.02
glotblastn


1464
LNU45
eggplant|10v1|FS003716
3416
501
93
globlastp


1465
LNU45
oat|10v1|GO582478
3417
501
93
globlastp


1466
LNU45
orobanche|10v1|SRR023495S0023362
3418
501
93
globlastp


1467
LNU45
physcomitrella|10v1|AW126791
3419
501
93
globlastp


1468
LNU45
physcomitrella|10v1|AW126917
3419
501
93
globlastp


1469
LNU45
physcomitrella|10v1|AW127011
3419
501
93
globlastp


1470
LNU45
apple|gb157.3|EB115463
3420
501
93
globlastp


1471
LNU45
b_rapa|gb162|EX051142
3421
501
93
globlastp


1472
LNU45
banana|gb167|FL658637
3422
501
93
globlastp


1473
LNU45
barley|gb157SOLEXA|AL512188
3417
501
93
globlastp


1474
LNU45
barley|gb157SOLEXA|BE421731
3417
501
93
globlastp


1475
LNU45
brachypodium|09v1|DV471589
3417
501
93
globlastp


1476
LNU45
brachypodium|gb169|BE399584
3417
501
93
globlastp


1477
LNU45
brachypodium|09v1|DV471799
3423
501
93
globlastp


1478
LNU45
brachypodium|gb169|BE416572
3423
501
93
globlastp


1479
LNU45
cenchrus|gb166|BM084666
3424
501
93
globlastp


1480
LNU45
dandelion|gb161|DY809678
3425
501
93
globlastp


1481
LNU45
dandelion|gb161|DY838552
3425
501
93
globlastp


1482
LNU45
ginger|gb164|DY361313
3426
501
93
globlastp


1483
LNU45
lettuce|10v1|DW044248
3427
501
93
globlastp


1484
LNU45
lettuce|gb157.2|DW044248
3427
501
93
globlastp


1485
LNU45
lettuce|gb157.2|DW103448
3427
501
93
globlastp


1486
LNU45
lettuce|gb157.2|DW126058
3427
501
93
globlastp


1487
LNU45
lettuce|gb157.2|DW145140
3427
501
93
globlastp


1488
LNU45
leymus|gb166|CN465799
3417
501
93
globlastp


1489
LNU45
maize|gb170|LLDQ245642
3417
501
93
globlastp


1490
LNU45
oat|10v1|CN817047
3417
501
93
globlastp


1491
LNU45
oat|gb164|CN817047
3417
501
93
globlastp


1492
LNU45
petunia|gb171|CV299912
3428
501
93
globlastp


1493
LNU45
physcomitrella|gb157|AW126791
3419
501
93
globlastp


1494
LNU45
physcomitrella|gb157|AW126917
3419
501
93
globlastp


1495
LNU45
pine|gb157.2|AW754553
3429
501
93
globlastp


1496
LNU45
poppy|gb166|FE965482
3430
501
93
globlastp


1497
LNU45
pseudoroegneria|gb167|FF351733
3417
501
93
globlastp


1498
LNU45
radish|gb164|FD579539
3431
501
93
globlastp


1499
LNU45
rye|gb164|BE494281
3417
501
93
globlastp


1500
LNU45
sugarcane|gb157.3|CA287147
3417
501
93
globlastp


1501
LNU45
tobacco|gb162|BQ842826
3418
501
93
globlastp


1502
LNU45
wheat|gb164|BE399584
3417
501
93
globlastp


1503
LNU45
wheat|gb164|BE443667
3417
501
93
globlastp


1504
LNU45
wheat|gb164|BF199537
3417
501
93
globlastp


1505
LNU45
wheat|gb164|BI751307
3417
501
93
globlastp


1506
LNU45
wheat|gb164|CA601804
3417
501
93
globlastp


1507
LNU45
lettuce|10v1|DW103448
3427
501
93
globlastp


1508
LNU45
orobanche|10v1|SRR023189S0090142
3432
501
92
globlastp


1509
LNU45
millet|09v1|EVO454PM260711
3433
501
91.9
globlastp


1510
LNU45
oat|10v1|GO586209
3434
501
91.9
globlastp


1511
LNU45
physcomitrella|10v1|AW155989
3435
501
91.9
globlastp


1512
LNU45
sorghum|09v1|SB01G008260
3433
501
91.9
globlastp


1513
LNU45
sugarcane|10v1|CA069593
3433
501
91.9
globlastp


1514
LNU45
arabidopsis|gb165|AT2G45710
3436
501
91.9
globlastp


1515
LNU45
fescue|gb161|DT686196
3437
501
91.9
globlastp


1516
LNU45
lovegrass|gb167|DN481942
3438
501
91.9
globlastp


1517
LNU45
lovegrass|gb167|EH188789
3439
501
91.9
globlastp


1518
LNU45
lovegrass|gb167|EH192368
3440
501
91.9
globlastp


1519
LNU45
maize|gb170|AI622704
3433
501
91.9
globlastp


1520
LNU45
maize|gb170|AI973383
3433
501
91.9
globlastp


1521
LNU45
maize|gb170|LLBI361219
3433
501
91.9
globlastp


1522
LNU45
maize|gb170|T23373
3433
501
91.9
globlastp


1523
LNU45
millet|09v1|EB410946
3441
501
91.9
globlastp


1524
LNU45
rice|gb170|OS02G27769
3442
501
91.9
globlastp


1525
LNU45
rose|gb157.2|EC587400
3443
501
91.9
globlastp


1526
LNU45
sorghum|09v1|SB04G018990
3433
501
91.9
globlastp


1527
LNU45
switchgrass|gb167|FE610323
3433
501
91.9
globlastp


1528
LNU45
switchgrass|gb167|FE616250
3433
501
91.9
globlastp


1529
LNU45
switchgrass|gb167|FE631460
3433
501
91.9
globlastp


1530
LNU45
switchgrass|gb167|FL725078
3433
501
91.9
globlastp


1531
LNU45
switchgrass|gb167|FL741521
3433
501
91.9
globlastp


1532
LNU45
switchgrass|gb167|FL849448
3433
501
91.9
globlastp


1533
LNU45
switchgrass|gb167|FL940045
3433
501
91.9
globlastp


1534
LNU45
wheat|gb164|CA486248
3433
501
91.9
globlastp


1535
LNU45
sugarcane|10v1|BQ535468
3433
501
91.9
globlastp


1536
LNU45
sugarcane|10v1|BQ535613
3433
501
91.9
globlastp


1537
LNU45
orobanche|10v1|SRR023189S0004445
3444
501
90.7
globlastp


1538
LNU45
physcomitrella|10v1|BJ164117
3445
501
90.7
globlastp


1539
LNU45
amborella|gb166|FD430338
3446
501
90.7
globlastp


1540
LNU45
banana|gb167|DN239917
3447
501
90.7
glotblastn


1541
LNU45
cacao|gb167|CU493520
3448
501
90.7
globlastp


1542
LNU45
cenchrus|gb166|EB660456
3449
501
90.7
globlastp


1543
LNU45
dandelion|gb161|DY834568
3450
501
90.7
globlastp


1544
LNU45
kiwi|gb166|FG418840
3451
501
90.7
glotblastn


1545
LNU45
marchantia|gb166|C95731
3452
501
90.7
globlastp


1546
LNU45
rice|gb170|OS04G27860
3453
501
90.7
globlastp


1547
LNU45
maize|gb170|LLFL411499
3454
501
89.53
glotblastn


1548
LNU45
switchgrass|gb167|FE624276
3455
501
89.53
glotblastn


1549
LNU45
rice|gb170|OS04G32710
3456
501
89.5
globlastp


1550
LNU45
spikemoss|gb165|DN838335
3457
501
89.5
globlastp


1551
LNU45
spikemoss|gb165|DN839110
3457
501
89.5
globlastp


1552
LNU45
fern|gb171|BP913163
3458
501
88.4
globlastp


1553
LNU45
ginseng|10v1|CN845877
3459
501
88.4
globlastp


1554
LNU45
ginseng|10v1|GR875257
3459
501
88.4
globlastp


1555
LNU45
rye|gb164|BE705802
3460
501
88.4
globlastp


1556
LNU45
canola|10v1|CD812394
3461
501
88.3
globlastp


1557
LNU45
fern|gb171|DK944513
3462
501
87.2
globlastp


1558
LNU45
b_juncea|gb164|EVGN00943108632248
3463
501
87.2
globlastp


1559
LNU45
chlamydomonas|gb162|X83694
3464
501
87.2
globlastp


1560
LNU45
volvox|gb162|X83694
3465
501
87.2
globlastp


1561
LNU45
citrus|gb166|CX663339
3466
501
86
globlastp


1562
LNU45
lovegrass|gb167|EH190160
3467
501
86
globlastp


1563
LNU45
mesostigma|gb166|DN254596
3468
501
86
globlastp


1564
LNU45
mesostigma|gb166|EC728430
3468
501
86
globlastp


1565
LNU45
arabidopsis_lyrata|09v1|JGIAL019416
3469
501
84.9
globlastp


1566
LNU45
heritiera|10v1|SRR005794S0004655
3470
501
84.9
globlastp


1567
LNU45
arabidopsis|gb165|AT3G61111
3471
501
82.6
globlastp


1568
LNU45
rye|gb164|BF146222
3472
501
82.6
globlastp


1569
LNU45
wheat|gb164|CA606076
3473
501
81.7
globlastp


1570
LNU45
solanum_phureja|09v1|SPHCRPSP011443
3474
501
81.4
glotblastn


1571
LNU45
citrus|gb166|DY261826
3475
501
81.4
globlastp


1572
LNU45
ostreococcus|gb162|XM001421510
3476
501
81.4
glotblastn


1573
LNU45
spruce|gb162|ES252989
3477
501
81.4
globlastp


1574
LNU45
switchgrass|gb167|GD026676
3478
501
81.4
glotblastn


1575
LNU45
coffea|10v1|GR981069
—
501
81.4
glotblastn


1576
LNU46
soybean|gb168|AW428695
3479
502
97.5
globlastp


1577
LNU46
cowpea|gb166|FF399962
3480
502
97
globlastp


1578
LNU46
lotus|09v1|AW428695
3481
502
96.1
globlastp


1579
LNU46
bean|gb167|CA898025
3482
502
96.1
globlastp


1580
LNU46
citrus|gb166|CF418615
3483
502
95.7
globlastp


1581
LNU46
cotton|gb164|CO109391
3484
502
95.7
globlastp


1582
LNU46
grape|gb160|BQ795999
3485
502
95.7
globlastp


1583
LNU46
cucumber|09v1|BGI454G0029699
3486
502
95.4
globlastp


1584
LNU46
cowpea|gb166|FF400935
3487
502
95.4
globlastp


1585
LNU46
poplar|10v1|BI120740
3488
502
95.4
globlastp


1586
LNU46
poplar|gb170|BI120740
3488
502
95.4
globlastp


1587
LNU46
cucumber|09v1|CV001012
3489
502
95.2
globlastp


1588
LNU46
castorbean|09v1|T15123
3490
502
95
globlastp


1589
LNU46
castorbean|gb160|T15123
3490
502
95
globlastp


1590
LNU46
kiwi|gb166|FG426103
3491
502
95
globlastp


1591
LNU46
cassava|09v1|DB921974
3492
502
94.7
globlastp


1592
LNU46
cycas|gb166|CB089800
3493
502
94.7
globlastp


1593
LNU46
medicago|09v1|AL376549
3494
502
94.7
globlastp


1594
LNU46
medicago|gb157.2|AL376549
3494
502
94.7
globlastp


1595
LNU46
spruce|gb162|CO226322
3495
502
94.7
globlastp


1596
LNU46
millet|09v1|EVO454PM007367
3496
502
94.5
globlastp


1597
LNU46
artemisia|gb164|EY071317
3497
502
94.5
globlastp


1598
LNU46
cacao|gb167|CU477411
3498
502
94.5
globlastp


1599
LNU46
aquilegia|10v1|DR925421
3499
502
94.3
globlastp


1600
LNU46
aquilegia|gb157.3|DR925421
3499
502
94.3
globlastp


1601
LNU46
maize|gb170|AI932193
3500
502
94.3
globlastp


1602
LNU46
sorghum|09v1|SB01G010860
3501
502
94.3
globlastp


1603
LNU46
sorghum|gb161.crp|BG051224
3501
502
94.3
globlastp


1604
LNU46
poplar|10v1|XM002304585
3502
502
94.1
globlastp


1605
LNU46
cynara|gb167|GE577142
3503
502
94.1
globlastp


1606
LNU46
lettuce|10v1|DW046496
3504
502
94.1
globlastp


1607
LNU46
lettuce|gb157.2|DW124917
3504
502
94.1
globlastp


1608
LNU46
oil_palm|gb166|ES273702
3505
502
94.1
globlastp


1609
LNU46
poplar|10v1|BI068703
3502
502
94.1
globlastp


1610
LNU46
poplar|gb170|BI068703
3502
502
94.1
globlastp


1611
LNU46
tomato|09v1|AI486841
3506
502
94.1
globlastp


1612
LNU46
tomato|gb164|AI486841
3506
502
94.1
globlastp


1613
LNU46
solanum_phureja|09v1|SPHAI486841
3507
502
93.8
globlastp


1614
LNU46
poplar|10v1|BI129155
3508
502
93.8
globlastp


1615
LNU46
poplar|gb170|BI129155
3508
502
93.8
globlastp


1616
LNU46
rice|gb170|OS03G49580
3509
502
93.8
globlastp


1617
LNU46
switchgrass|gb167|FE599427
3510
502
93.8
globlastp


1618
LNU46
switchgrass|gb167|FE604633
3511
502
93.8
globlastp


1619
LNU46
centaurea|gb166|EL934628
3512
502
93.6
globlastp


1620
LNU46
rice|gb170|OS07G39870
3513
502
93.6
globlastp


1621
LNU46
sugarcane|10v1|CA089292
3514
502
93.6
globlastp


1622
LNU46
sugarcane|gb157.3|CA089292
3514
502
93.6
globlastp


1623
LNU46
monkeyflower|10v1|GO963384
3515
502
93.4
globlastp


1624
LNU46
apple|gb157.3|CN493322
3516
502
93.4
globlastp


1625
LNU46
apple|gb171|CN493322
3516
502
93.4
globlastp


1626
LNU46
maize|gb170|BG354242
3517
502
93.4
globlastp


1627
LNU46
sorghum|09v1|SB02G037930
3518
502
93.4
globlastp


1628
LNU46
sorghum|gb161.crp|AW671733
3518
502
93.4
globlastp


1629
LNU46
strawberry|gb164|DY666824
3519
502
93.4
globlastp


1630
LNU46
chestnut|gb170|SRR006295S0030165
3520
502
93.1
globlastp


1631
LNU46
cotton|gb164|BG439937
3521
502
93.1
globlastp


1632
LNU46
oak|gb170|DB998392
3522
502
93.1
globlastp


1633
LNU46
arabidopsis|gb165|AT3G2661
3523
502
92.9
globlastp


1634
LNU46
cotton|gb164|AI054657
3524
502
92.9
globlastp


1635
LNU46
pine|10v1|AI812442
3525
502
92.7
globlastp


1636
LNU46
pine|gb157.2|AI812442
3525
502
92.7
globlastp


1637
LNU46
prunus|gb167|BU039215
3526
502
92.7
globlastp


1638
LNU46
soybean|gb168|AW691393
3527
502
92.7
globlastp


1639
LNU46
pepper|gb171|BM063924
3528
502
92.4
globlastp


1640
LNU46
citrus|gb166|CB291077
3529
502
92.4
globlastp


1641
LNU46
sunflower|gb162|CD845824
3530
502
92.4
globlastp


1642
LNU46
tomato|09v1|BG129404
3531
502
92.4
globlastp


1643
LNU46
tomato|gb164|BG129404
3531
502
92.4
globlastp


1644
LNU46
arabidopsis_lyrata|09v1|JGIAL016770
3532
502
92.2
globlastp


1645
LNU46
apple|gb157.3|CN544908
3533
502
92.2
globlastp


1646
LNU46
apple|gb171|CN544908
3533
502
92.2
globlastp


1647
LNU46
barley|gb157SOLEXA|BE421791
3534
502
92.2
globlastp


1648
LNU46
cowpea|gb166|FF399895
3535
502
92.2
globlastp


1649
LNU46
oat|10v1|CN820661
3536
502
92
globlastp


1650
LNU46
solanum_phureja|09v1|SPHBG129404
3537
502
92
globlastp


1651
LNU46
potato|10v1|BF053654
3537
502
92
globlastp


1652
LNU46
potato|gb157.2|BF053654
3537
502
92
globlastp


1653
LNU46
soybean|gb168|AW428757
3538
502
91.8
globlastp


1654
LNU46
spruce|gb162|CO220375
3539
502
91.8
globlastp


1655
LNU46
triphysaria|gb164|EX991156
3540
502
91.8
globlastp


1656
LNU46
physcomitrella|10v1|BJ175132
3541
502
91.6
globlastp


1657
LNU46
physcomitrella|gb157|BJ175017
3541
502
91.6
globlastp


1658
LNU46
canola|10v1|CD824781
3542
502
91.5
globlastp


1659
LNU46
oat|10v1|BE439128
3543
502
91.5
globlastp


1660
LNU46
brachypodium|09v1|DV471205
3544
502
91.5
globlastp


1661
LNU46
brachypodium|gb169|BE446313
3544
502
91.5
globlastp


1662
LNU46
papaya|gb165|AM904175
3545
502
91.5
globlastp


1663
LNU46
sunflower|gb162|DY910709
3546
502
91.5
globlastp


1664
LNU46
barley|gb157SOLEXA|BE438955
3547
502
91.3
globlastp


1665
LNU46
canola|gb161|DY011567
3548
502
91.3
globlastp


1666
LNU46
fescue|gb161|DT700877
3549
502
91.3
globlastp


1667
LNU46
wheat|gb164|BU099476
3550
502
91.3
globlastp


1668
LNU46
physcomitrella|10v1|BY984317
3551
502
91.2
globlastp


1669
LNU46
physcomitrella|10v1|BQ827517
3552
502
90.9
globlastp


1670
LNU46
physcomitrella|gb157|BQ827517
3553
502
90.87
glotblastn


1671
LNU46
coffea|10v1|DV667361
3554
502
90.8
globlastp


1672
LNU46
potato|10v1|BF154263
3555
502
90.8
globlastp


1673
LNU46
potato|gb157.2|BF154263
3555
502
90.8
globlastp


1674
LNU46
dandelion|gb161|DY809008
3556
502
90.7
globlastp


1675
LNU46
canola|10v1|EE407094
3557
502
90.6
globlastp


1676
LNU46
pepper|gb171|CA523256
3558
502
90.6
globlastp


1677
LNU46
solanum_phureja|09v1|SPHAA824687
3559
502
90.6
globlastp


1678
LNU46
solanum_phureja|09v1|SPHAJ489160
3560
502
90.6
globlastp


1679
LNU46
canola|10v1|CD815302
3557
502
90.6
globlastp


1680
LNU46
canola|gb161|CD815302
3557
502
90.6
globlastp


1681
LNU46
medicago|09v1|AW691393
3561
502
90.6
globlastp


1682
LNU46
medicago|gb157.2|AW691393
3561
502
90.6
globlastp


1683
LNU46
pine|10v1|AW010603
3562
502
90.6
globlastp


1684
LNU46
tomato|09v1|AA824687
3563
502
90.6
globlastp


1685
LNU46
tomato|09v1|AJ489160
3564
502
90.4
globlastp


1686
LNU46
pine|gb157.2|AW010603
3565
502
90.4
globlastp


1687
LNU46
tomato|gb164|AA824687
3566
502
90.4
globlastp


1688
LNU46
potato|10v1|AJ489160
3567
502
90.39
glotblastn


1689
LNU46
arabidopsis_lyrata|09v1|JGIAL001354
3568
502
90.2
globlastp


1690
LNU46
monkeyflower|10v1|DV209146
3569
502
89.9
globlastp


1691
LNU46
arabidopsis|gb165|AT1G12910
3570
502
89.9
globlastp


1692
LNU46
coffea|gb157.2|DV667361
3571
502
89.9
globlastp


1693
LNU46
tobacco|gb162|CV016199
3572
502
89.5
globlastp


1694
LNU46
radish|gb164|EV534949
3573
502
89.47
glotblastn


1695
LNU46
physcomitrella|10v1|BJ175017
3574
502
89.4
globlastp


1696
LNU46
spikemoss|gb165|FE441264
3575
502
88.1
globlastp


1697
LNU46
arabidopsis_lyrata|09v1|JGIAL028997
3576
502
87.9
globlastp


1698
LNU46
arabidopsis|gb165|AT5G47880
3577
502
87.9
globlastp


1699
LNU46
spikemoss|gb165|FE441265
3578
502
87.6
globlastp


1700
LNU46
radish|gb164|EV569528
3579
502
87.5
globlastp


1701
LNU46
canola|10v1|CD814349
3580
502
87
globlastp


1702
LNU46
rice|gb170|OS01G71270
3581
502
87
globlastp


1703
LNU46
brachypodium|09v1|DV469404
3582
502
86.3
globlastp


1704
LNU46
brachypodium|gb169|BG606860
3582
502
86.3
globlastp


1705
LNU46
soybean|gb168|AL376550
3583
502
86.04
glotblastn


1706
LNU46
medicago|09v1|CRPMT036602
3584
502
83.5
globlastp


1707
LNU46
millet|09v1|CD724536
3585
502
83.1
globlastp


1708
LNU46
papaya|gb165|EX253193
3586
502
81.7
globlastp


1709
LNU46
switchgrass|gb167|FE602227
3587
502
80.9
globlastp


1710
LNU46
onion|gb162|CF441127
3588
502
80.55
glotblastn


1711
LNU46
sorghum|09v1|SB09G018630
3589
502
80.2
globlastp


1712
LNU46
sorghum|gb161.crp|BE362758
3589
502
80.2
globlastp


1713
LNU48
switchgrass|gb167|FE638123
3590
503
89
globlastp


1714
LNU48
brachypodium|09v1|DV472885
3591
503
88.37
glotblastn


1715
LNU48
sorghum|09v1|SB02G032700
3592
503
87.2
globlastp


1716
LNU48
sorghum|gb161.crp|AW256150
3593
503
86.82
glotblastn


1717
LNU48
maize|gb170|AW256150
3594
503
86.6
globlastp


1718
LNU51
sorghum|09v1|SB02G028140
3595
505
82.6
globlastp


1719
LNU51
sorghum|gb161.crp|AW520040
3595
505
82.6
globlastp


1720
LNU51
maize|gb170|AW060000
3596
505
82.2
globlastp


1721
LNU51
sugarcane|gb157.3|CA070485
3597
505
81.57
glotblastn


1722
LNU51
brachypodium|09v1|GT760634
3598
505
81.5
globlastp


1723
LNU51
barley|gb157.3|AL499810
3599
505
81.3
globlastp


1724
LNU51
barley|gb157SOLEXA|AL499810
3599
505
81.3
globlastp


1725
LNU51
sugarcane|10v1|CA070485
3600
505
81
globlastp


1726
LNU52
sugarcane|gb157.3|CA075246
3601
506
92.6
globlastp


1727
LNU52
sorghum|09v1|SB01G047930
3602
506
92.4
globlastp


1728
LNU52
sorghum|gb1618.crp|AW433438
3602
506
92.4
globlastp


1729
LNU52
brachypodium|gb169|BE400443
3603
506
92.01
glotblastn


1730
LNU52
brachypodium|09v1|DV470543
3604
506
91.8
globlastp


1731
LNU52
maize|gb170|BE123353
3605
506
91.6
globlastp


1732
LNU52
switchgrass|gb167|DN141859
3606
506
91
globlastp


1733
LNU52
switchgrass|gb167|DN147113
3607
506
90.5
globlastp


1734
LNU52
maize|gb170|AW076488
3608
506
89.5
globlastp


1735
LNU52
wheat|gb164|BE400443
3609
506
89
globlastp


1736
LNU52
oat|10v1|GR316421
3610
506
88
globlastp


1737
LNU52
brachypodium|gb169|BE399305
3611
506
87.2
globlastp


1738
LNU52
barley|gb157SOLEXA|BF623122
3612
506
87.1
globlastp


1739
LNU52
wheat|gb164|BE399305
3613
506
87.1
globlastp


1740
LNU52
barley|gb157SOLEXA|AL505656
3614
506
86.5
globlastp


1741
LNU52
brachypodium|09v1|GT766862
3615
506
85.5
globlastp


1742
LNU56
bean|gb167|CA909969
3616
510
88.8
globlastp


1743
LNU56
cowpea|gb166|FC458698
3617
510
88.78
glotblastn


1744
LNU56
medicago|09v1|LLEX523937
3618
510
85.6
globlastp


1745
LNU56
chickpea|09v2|GR401628
3619
510
83.6
globlastp


1746
LNU56
peanut|gb171|ES762633
3620
510
83
globlastp


1747
LNU56
medicago|09v1|AL374335
3621
510
82.1
globlastp


1748
LNU56
medicago|gb157.2|AL374335
3621
510
82.1
globlastp


1749
LNU56
prunus|gb167|BU043945
3622
510
81
globlastp


1750
LNU56
chestnut|gb170|SRR006295S0008375
3623
510
80.5
globlastp


1751
LNU56
poplar|gb170|BU876352
3624
510
80.5
globlastp


1752
LNU56
cucumber|09v1|AM717347
3625
510
80
globlastp


1753
LNU56
poplar|10v1|BU876352
3626
510
80
globlastp


1754
LNU57
wheat|gb164|BE431144
3627
511
86.8
globlastp


1755
LNU57
pseudoroegneria|gb167|FF351563
3628
511
85.4
globlastp


1755
LNU81
pseudoroegneria|gb167|FF351563
3628
728
85.9
globlastp


1756
LNU60
brachypodium|09v1|SRR031797S0000144
3629
514
88.2
globlastp


1756
LNU84
brachypodium|09v1|SRR031797S0000144
3629
533
80.8
globlastp


1756
LNU65
brachypodium|09v1|SRR031797S0000144
3629
722
80.5
globlastp


1757
LNU60
maize|gb170|AW562715
3630
514
80.6
globlastp


1757
LNU84
maize|gb170|AW562715
3630
533
90.9
globlastp


1758
LNU61
brachypodium|09v1|DV488685
3631
515
86.8
globlastp


1759
LNU63
pseudoroegneria|gb167|FF366744
3632
516
93.8
globlastp


1760
LNU63
wheat|gb164|CD937806
3633
516
90.1
globlastp


1761
LNU63
wheat|gb164|BF485055
3634
516
89.3
globlastp


1762
LNU63
barley|gb157.3|AL508288
3635
516
82.3
globlastp


1763
LNU63
barley|gb157SOLEXA|AL508288
3635
516
82.3
globlastp


1764
LNU63
leymus|gb166|EG402462
3636
516
81.8
globlastp


1765
LNU64
wheat|gb164|BG907753
3637
517
97.1
globlastp


1766
LNU64
wheat|gb164|BQ842100
3638
517
93.1
globlastp


1766
LNU98
wheat|gb164|BQ842100
3638
734
80.57
glotblastn


1767
LNU64
brachypodium|09v1|GT761032
3639
517
88.25
glotblastn


1768
LNU64
switchgrass|gb167|FE631036
3640
517
85.8
globlastp


1768
LNU98
switchgrass|gb167|FE631036
3640
734
84.2
globlastp


1769
LNU64
maize|gb170|AW438149
3641
517
84.3
globlastp


1769
LNU98
maize|gb170|AW438149
3641
734
81.1
globlastp


1770
LNU64
rice|gb170|OS06G05700
3642
517
83.9
globlastp


1770
LNU98
rice|gb170|OS06G05700
3642
734
81.1
globlastp


1771
LNU64
brachypodium|gb169|BE591591
3643
517
80.46
glotblastn


1772
LNU67
brachypodium|09v1|GT769610
3644
519
88.4
globlastp


1773
LNU67
barley|gb157.3|AL510475
3645
519
86.13
glotblastn


1774
LNU67
barley|gb157SOLEXA|AL510475
3645
519
86.13
glotblastn


1775
LNU67
sorghum|09v1|SB01G029600
3646
519
85.4
globlastp


1776
LNU67
sugarcane|10v1|CA073684
3647
519
85.4
globlastp


1777
LNU67
sugarcane|gb157.3|CA073684
3648
519
85.4
globlastp


1778
LNU67
sorghum|gb161.crp|AI586547
3646
519
85.4
globlastp


1779
LNU67
maize|gb170|AI586547
3649
519
84.2
globlastp


1780
LNU67
brachypodium|gb169|BG418808
3650
519
81.3
globlastp


1781
LNU67
switchgrass|gb167|FE612667
3651
519
80.3
globlastp


1782
LNU69
brachypodium|09v1|DV472747
3652
521
87.1
globlastp


1783
LNU69
oat|10v1|GR321530
3653
521
86.36
glotblastn


1784
LNU69
oat|10v1|GO589136
3654
521
85.9
globlastp


1785
LNU69
wheat|gb164|BE637681
3655
521
85.5
globlastp


1786
LNU69
barley|gb157SOLEXA|BI950025
3656
521
84.5
globlastp


1787
LNU69
barley|gb157.3|BI950025
3657
521
84.2
globlastp


1788
LNU69
pseudoroegneria|gb167|FF340678
3658
521
84.2
globlastp


1789
LNU69
brachypodium|gb169|BE637681
3659
521
81.6
globlastp


1790
LNU69
sorghum|gb161.crp|BG049299
3660
521
81.2
globlastp


1791
LNU69
maize|gb170|AI861497
3661
521
81.2
globlastp


1792
LNU69
sorghum|09v1|SB07G026190
3660
521
81.2
globlastp


1793
LNU69
sugarcane|10v1|CA090545
3662
521
81.2
globlastp


1794
LNU69
sugarcane|gb157.3|CA090545
3663
521
80.8
globlastp


1795
LNU71
rice|gb170|OS01G72240
3664
523
83
globlastp


1796
LNU71
oat|10v1|GR359520
3665
523
82.39
glotblastn


1797
LNU71
sorghum|09v1|SB03G045930
3666
523
82.1
globlastp


1798
LNU71
sorghum|gb161.crp|AI396343
3666
523
82.1
globlastp


1799
LNU71
sorghum|09v1|SB03G045940
3667
523
81.4
globlastp


1800
LNU71
maize|gb170|AI396343
3668
523
81.1
globlastp


1801
LNU71
maize|gb170|AI668476
3669
523
80.6
globlastp


1802
LNU73
sorghum|09v1|SB01G031850
3670
525
83.6
globlastp


1803
LNU73
sorghum|gb161.crp|AI665094
3670
525
83.6
globlastp


1804
LNU73
brachypodium|09v1|DV478838
3671
525
81.2
globlastp


1805
LNU73
brachypodium|gb169|BE585945
3672
525
80.9
globlastp


1806
LNU73
oat|10v1|GR315201
3673
525
80.8
globlastp


1807
LNU73
leymus|gb166|EG395112
3674
525
80.6
globlastp


1808
LNU73
wheat|gb164|BE213619
3675
525
80.6
globlastp


1809
LNU73
maize|gb170|AI600420
3676
525
80.2
globlastp


1810
LNU73
barley|gb157SOLEXA|AL507770
3677
525
80
globlastp


1811
LNU74
poplar|gb170|AI164221
3678
526
94.8
globlastp


1812
LNU74
poplar|10v1|AI164221
3679
526
94
globlastp


1813
LNU74
cassava|09v1|DV441703
3680
526
93.3
globlastp


1814
LNU74
castorbean|09v1|EE256800
3681
526
93.3
globlastp


1815
LNU74
cassava|09v1|BM260313
3682
526
92.5
globlastp


1816
LNU74
cassava|gb164|BM260313
3682
526
92.5
globlastp


1817
LNU74
cassava|gb164|DV441703
3683
526
92.5
globlastp


1818
LNU74
castorbean|gb160|EE256800
3684
526
92.5
globlastp


1819
LNU74
banana|gb167|ES432444
3685
526
91.8
globlastp


1820
LNU74
citrus|gb166|BQ624628
3686
526
91.8
globlastp


1821
LNU74
papaya|gb165|EX290028
3687
526
91.8
globlastp


1822
LNU74
banana|gb167|FL665867
3688
526
91
globlastp


1823
LNU74
papaya|gb165|AM903637
3689
526
91
globlastp


1824
LNU74
poplar|10v1|BU817024
3690
526
91
globlastp


1825
LNU74
poplar|gb170|BU817024
3690
526
91
globlastp


1826
LNU74
kiwi|gb166|FG488574
3691
526
90.4
globlastp


1827
LNU74
citrus|gb166|BQ624990
3692
526
90.3
globlastp


1828
LNU74
poplar|10v1|BU813474
3693
526
90.3
globlastp


1829
LNU74
poplar|gb170|BU813474
3693
526
90.3
globlastp


1830
LNU74
bruguiera|gb166|BP946426
3694
526
89.6
globlastp


1831
LNU74
sesame|gb157.2|BU668642
3695
526
89.6
globlastp


1832
LNU74
sesame|10v1|BU667940
3696
526
88.81
glotblastn


1833
LNU74
jatropha|09v1|FM893408
3697
526
88.8
globlastp


1834
LNU74
cacao|gb167|CU491187
3698
526
88.8
globlastp


1835
LNU74
castorbean|09v1|XM002529794
3699
526
88.8
globlastp


1836
LNU74
ginger|gb164|DY377849
3700
526
88.8
globlastp


1837
LNU74
grape|gb160|BQ797018
3701
526
88.8
globlastp


1838
LNU74
spurge|gb161|BE095323
3702
526
88.8
globlastp


1839
LNU74
cleome_gynandra|10v1|SRR015532S0006733
3703
526
88.1
globlastp


1840
LNU74
heritiera|10v1|SRR005795S0013040
3704
526
88.1
globlastp


1841
LNU74
aquilegia|10v1|DR915465
3705
526
88.1
globlastp


1842
LNU74
basilicum|10v1|DY341993
3706
526
88.1
globlastp


1843
LNU74
castorbean|gb160|MDL28492M000475
3707
526
88.1
globlastp


1844
LNU74
grape|gb160|CB004623
3708
526
88.1
globlastp


1845
LNU74
centaurea|gb166|EH742056
3709
526
87.9
globlastp


1846
LNU74
apple|gb171|CN581387
3710
526
87.3
globlastp


1847
LNU74
solanum_phureja|09v1|SPHBG123695
3711
526
87.3
globlastp


1848
LNU74
apple|gb157.3|CN444719
3712
526
87.3
globlastp


1849
LNU74
apple|gb171|CN444719
3712
526
87.3
globlastp


1850
LNU74
ginger|gb164|DY357017
3713
526
87.3
globlastp


1851
LNU74
potato|gb157.2|BF153790
3711
526
87.3
globlastp


1852
LNU74
potato|gb157.2|BG591609
3711
526
87.3
globlastp


1853
LNU74
spurge|gb161|BI993550
3714
526
87.3
globlastp


1854
LNU74
spurge|gb161|DV120560
3715
526
87.3
globlastp


1855
LNU74
thellungiella|gb167|DN772768
3716
526
87.3
globlastp


1856
LNU74
triphysaria|gb164|EX991312
3717
526
87.3
globlastp


1857
LNU74
triphysaria|gb164|EX992098
3718
526
87.3
globlastp


1858
LNU74
triphysaria|gb164|EX993270
3719
526
87.3
globlastp


1859
LNU74
potato|10v1|BF153790
3711
526
87.3
globlastp


1860
LNU74
canola|10v1|BQ704346
3720
526
86.6
globlastp


1861
LNU74
canola|10v1|CD812655
3720
526
86.6
globlastp


1862
LNU74
canola|10v1|CD817750
3720
526
86.6
globlastp


1863
LNU74
canola|10v1|CD820979
3720
526
86.6
globlastp


1864
LNU74
canola|10v1|CD822847
3720
526
86.6
globlastp


1865
LNU74
canola|10v1|CD839803
3720
526
86.6
globlastp


1866
LNU74
canola|10v1|CD840413
3720
526
86.6
globlastp


1867
LNU74
cleome_gynandra|10v1|SRR015532S0001567
3721
526
86.6
globlastp


1868
LNU74
liquorice|gb171|FS239300
3722
526
86.6
globlastp


1869
LNU74
monkeyflower|10v1|DV211742
3723
526
86.6
globlastp


1870
LNU74
orobanche|10v1|SRR023189S0022715
3724
526
86.6
globlastp


1871
LNU74
rhizophora|10v1|SRR005792S0001265
3725
526
86.6
globlastp


1872
LNU74
salvia|10v1|CV170183
3726
526
86.6
globlastp


1873
LNU74
b_juncea|gb164|EVGN00065811011380
3720
526
86.6
globlastp


1874
LNU74
b_juncea|gb164|EVGN00096612141341
3720
526
86.6
globlastp


1875
LNU74
b_juncea|gb164|EVGN00247926600394
3720
526
86.6
globlastp


1876
LNU74
b_juncea|gb164|EVGN00581615062911
3720
526
86.6
globlastp


1877
LNU74
b_juncea|gb164|EVGN01024809191906
3720
526
86.6
globlastp


1878
LNU74
b_oleracea|gb161|DY025798
3720
526
86.6
globlastp


1879
LNU74
b_oleracea|gb161|DY026115
3727
526
86.6
globlastp


1880
LNU74
b_rapa|gb162|CA992036
3720
526
86.6
globlastp


1881
LNU74
b_rapa|gb162|CX265596
3720
526
86.6
globlastp


1882
LNU74
b_rapa|gb162|CX265986
3720
526
86.6
globlastp


1883
LNU74
b_rapa|gb162|DY013411
3720
526
86.6
globlastp


1884
LNU74
b_rapa|gb162|L35798
3720
526
86.6
globlastp


1885
LNU74
cacao|gb167|CU473549
3728
526
86.6
globlastp


1886
LNU74
canola|gb161|CD812655
3720
526
86.6
globlastp


1887
LNU74
canola|gb161|CD817750
3720
526
86.6
globlastp


1888
LNU74
canola|gb161|CD818582
3720
526
86.6
globlastp


1889
LNU74
canola|gb161|CD820979
3720
526
86.6
globlastp


1890
LNU74
canola|gb161|CD822847
3720
526
86.6
globlastp


1891
LNU74
canola|gb161|CD839803
3720
526
86.6
globlastp


1892
LNU74
canola|gb161|CN731800
3720
526
86.6
globlastp


1893
LNU74
cotton|gb164|AI726252
3729
526
86.6
globlastp


1894
LNU74
cotton|gb164|BE054575
3730
526
86.6
globlastp


1895
LNU74
cotton|gb164|BF268166
3731
526
86.6
globlastp


1896
LNU74
ginger|gb164|DY347722
3732
526
86.6
globlastp


1897
LNU74
kiwi|gb166|FG426073
3733
526
86.6
globlastp


1898
LNU74
maize|gb170|LLDQ244555
3720
526
86.6
globlastp


1899
LNU74
oil_palm|gb166|EL684957
3734
526
86.6
globlastp


1900
LNU74
pepper|gb171|CA521239
3735
526
86.6
globlastp


1901
LNU74
radish|gb164|EV524672
3720
526
86.6
globlastp


1902
LNU74
radish|gb164|EV535208
3720
526
86.6
globlastp


1903
LNU74
radish|gb164|EV536989
3720
526
86.6
globlastp


1904
LNU74
radish|gb164|EV538098
3720
526
86.6
globlastp


1905
LNU74
radish|gb164|EV544188
3720
526
86.6
globlastp


1906
LNU74
radish|gb164|EX751329
3720
526
86.6
globlastp


1907
LNU74
radish|gb164|EX904913
3720
526
86.6
globlastp


1908
LNU74
radish|gb164|T25169
3720
526
86.6
globlastp


1909
LNU74
tamarix|gb166|CV791366
3736
526
86.6
globlastp


1910
LNU74
tomato|09v1|BG128242
3737
526
86.6
globlastp


1911
LNU74
tomato|gb164|BG128242
3737
526
86.6
globlastp


1912
LNU74
triphysaria|gb164|CB815081
3738
526
86.6
globlastp


1913
LNU74
sesame|gb157.2|BU667940
3739
526
86.57
glotblastn


1914
LNU74
cotton|gb164|BE052419
3740
526
86
globlastp


1915
LNU74
cleome_gynandra|10v1|SRR015532S0035591
3741
526
85.8
globlastp


1916
LNU74
eggplant|10v1|FS014035
3742
526
85.8
globlastp


1917
LNU74
monkeyflower|10v1|DV208750
3743
526
85.8
globlastp


1918
LNU74
orobanche|10v1|SRR023189S0004356
3744
526
85.8
globlastp


1919
LNU74
potato|10v1|BG589981
3745
526
85.8
globlastp


1920
LNU74
rhizophora|10v1|SRR005793S0025926
3746
526
85.8
globlastp


1921
LNU74
solanum_phureja|09v1|SPHBG128242
3745
526
85.8
globlastp


1922
LNU74
solanum_phureja|09v1|SPHBG131313
3747
526
85.8
globlastp


1923
LNU74
antirrhinum|gb166|AJ558344
3748
526
85.8
globlastp


1924
LNU74
avocado|10v1|CV461343
3749
526
85.8
globlastp


1925
LNU74
banana|gb167|DN239293
3750
526
85.8
globlastp


1926
LNU74
basilicum|gb157.3|DY341993
3751
526
85.8
globlastp


1927
LNU74
catharanthus|gb166|EG554531
3752
526
85.8
globlastp


1928
LNU74
chestnut|gb170|SRR006295S0004660
3753
526
85.8
globlastp


1929
LNU74
chestnut|gb170|SRR006295S0005476
3754
526
85.8
globlastp


1930
LNU74
kiwi|gb166|FG412937
3755
526
85.8
globlastp


1931
LNU74
kiwi|gb166|FG418868
3756
526
85.8
globlastp


1932
LNU74
oak|gb170|DB996491
3754
526
85.8
globlastp


1933
LNU74
oak|gb170|DN950062
3753
526
85.8
globlastp


1934
LNU74
oil_palm|gb166|CN600372
3757
526
85.8
globlastp


1935
LNU74
pineapple|10v1|DT336533
3758
526
85.8
globlastp


1936
LNU74
potato|10v1|BG350007
3747
526
85.8
globlastp


1937
LNU74
potato|gb157.2|BG350007
3747
526
85.8
globlastp


1938
LNU74
potato|gb157.2|BG589981
3745
526
85.8
globlastp


1939
LNU74
prunus|gb167|CB819261
3759
526
85.8
globlastp


1940
LNU74
prunus|gb167|CB819309
3760
526
85.8
globlastp


1941
LNU74
radish|gb164|EV543656
3761
526
85.8
globlastp


1942
LNU74
strawberry|gb164|DY674514
3762
526
85.8
globlastp


1943
LNU74
tomato|09v1|BG123695
3763
526
85.8
globlastp


1944
LNU74
tomato|gb164|BG123695
3763
526
85.8
globlastp


1945
LNU74
tomato|09v1|BG131313
3764
526
85.8
globlastp


1946
LNU74
tomato|gb164|BG131313
3764
526
85.8
globlastp


1947
LNU74
cleome_spinosa|10v1|SRR015531S0000037
3765
526
85.1
globlastp


1948
LNU74
cleome_spinosa|10v1|SRR015531S0001284
3766
526
85.1
globlastp


1949
LNU74
cleome_spinosa|10v1|SRR015531S0008654
3767
526
85.1
globlastp


1950
LNU74
eggplant|10v1|FS000830
3768
526
85.1
globlastp


1951
LNU74
ginseng|10v1|CN846360
3769
526
85.1
globlastp


1952
LNU74
ipomoea_batatas|10v1|BU691765
3770
526
85.1
globlastp


1953
LNU74
ipomoea_nil|10v1|BJ560886
3771
526
85.1
globlastp


1954
LNU74
salvia|10v1|CV169031
3772
526
85.1
globlastp


1955
LNU74
apple|gb157.3|CN443979
3773
526
85.1
globlastp


1956
LNU74
apple|gb171|CN443979
3773
526
85.1
globlastp


1957
LNU74
apple|gb171|CN579105
3774
526
85.1
globlastp


1958
LNU74
arabidopsis|gb165|AT2G20450
3775
526
85.1
globlastp


1959
LNU74
arabidopsis|gb165|AT4G27090
3776
526
85.1
globlastp


1960
LNU74
b_oleracea|gb161|DY025895
3777
526
85.1
globlastp


1961
LNU74
cowpea|gb166|FF393525
3778
526
85.1
globlastp


1962
LNU74
nuphar|gb166|CD475921
3779
526
85.1
globlastp


1963
LNU74
oil_palm|gb166|EL689892
3780
526
85.1
globlastp


1964
LNU74
pepper|gb157.2|BM062339
3781
526
85.1
globlastp


1965
LNU74
pepper|gb171|BM062339
3781
526
85.1
globlastp


1966
LNU74
poppy|gb166|FE964687
3782
526
85.1
globlastp


1967
LNU74
tobacco|gb162|BQ842878
3783
526
85.1
globlastp


1968
LNU74
tobacco|gb162|BQ842898
3784
526
85.1
globlastp


1969
LNU74
tobacco|gb162|CV017540
3783
526
85.1
globlastp


1970
LNU74
antirrhinum|gb166|AJ786986
3785
526
85.07
glotblastn


1971
LNU74
soybean|gb168|AW171724
3786
526
84.33
glotblastn


1972
LNU74
arabidopsis_lyrata|09v1|JGIAL025381
3787
526
84.3
globlastp


1973
LNU74
flax|09v1|EU830250
3788
526
84.3
globlastp


1974
LNU74
ipomoea_nil|10v1|BJ554450
3789
526
84.3
globlastp


1975
LNU74
ipomoea_nil|10v1|BJ554544
3790
526
84.3
globlastp


1976
LNU74
avocado|10v1|CV460831
3791
526
84.3
globlastp


1977
LNU74
avocado|gb164|CV460831
3791
526
84.3
globlastp


1978
LNU74
avocado|gb164|CV461343
3792
526
84.3
globlastp


1979
LNU74
bean|gb167|CA904056
3793
526
84.3
globlastp


1980
LNU74
canola|10v1|EE451770
3794
526
84.3
globlastp


1981
LNU74
cotton|gb164|BE052734
3795
526
84.3
globlastp


1982
LNU74
ginger|gb164|DY352377
3796
526
84.3
globlastp


1983
LNU74
ipomoea|gb157.2|BJ554450
3789
526
84.3
globlastp


1984
LNU74
ipomoea|gb157.2|BJ554544
3790
526
84.3
globlastp


1985
LNU74
lettuce|gb157.2|DW145600
3797
526
84.3
globlastp


1986
LNU74
liriodendron|gb166|CK748217
3798
526
84.3
globlastp


1987
LNU74
maize|gb170|LLFL032821
3799
526
84.3
globlastp


1988
LNU74
onion|gb162|BQ579932
3800
526
84.3
globlastp


1989
LNU74
pepper|gb171|BM061235
3801
526
84.3
globlastp


1990
LNU74
pepper|gb157.2|CA521239
3802
526
84.3
globlastp


1991
LNU74
petunia|gb166|CV296857
3803
526
84.3
globlastp


1992
LNU74
petunia|gb171|CV296857
3803
526
84.3
globlastp


1993
LNU74
poppy|gb166|FE965604
3804
526
84.3
globlastp


1994
LNU74
rose|10v1|EC588002
3805
526
84.3
globlastp


1995
LNU74
rose|gb157.2|EC588002
3805
526
84.3
globlastp


1996
LNU74
strawberry|gb164|CO379805
3806
526
84.3
globlastp


1997
LNU74
triphysaria|gb164|EX996679
3807
526
84.3
globlastp


1998
LNU74
walnuts|gb166|EL900249
3808
526
84.3
globlastp


1999
LNU74
apple|gb171|CN444601
3809
526
83.6
globlastp


2000
LNU74
bean|gb167|CA897601
3810
526
83.6
globlastp


2001
LNU74
bean|gb167|CB543012
3811
526
83.6
globlastp


2002
LNU74
beet|gb162|BI095986
3812
526
83.6
globlastp


2003
LNU74
cowpea|gb166|FF385283
3813
526
83.6
globlastp


2004
LNU74
ipomoea|gb157.2|BU691765
3814
526
83.6
globlastp


2005
LNU74
lettuce|gb157.2|DW043837
3815
526
83.6
globlastp


2006
LNU74
lettuce|gb157.2|DW048497
3816
526
83.6
globlastp


2007
LNU74
lettuce|10v1|DW074549
3817
526
83.6
globlastp


2008
LNU74
lettuce|gb157.2|DW074549
3817
526
83.6
globlastp


2009
LNU74
lettuce|10v1|DW074794
3818
526
83.6
globlastp


2010
LNU74
lettuce|gb157.2|DW074794
3818
526
83.6
globlastp


2011
LNU74
lettuce|gb157.2|DW104050
3815
526
83.6
globlastp


2012
LNU74
lettuce|gb157.2|DW151136
3816
526
83.6
globlastp


2013
LNU74
lotus|09v1|CB827421
3819
526
83.6
globlastp


2014
LNU74
lotus|gb157.2|CB827421
3819
526
83.6
globlastp


2015
LNU74
pepper|gb157.2|BM061235
3820
526
83.6
globlastp


2016
LNU74
soybean|gb168|AL373135
3821
526
83.6
globlastp


2017
LNU74
soybean|gb168|CA904059
3822
526
83.6
globlastp


2018
LNU74
lettuce|10v1|DW043837
3815
526
83.6
globlastp


2019
LNU74
lettuce|10v1|DW048497
3816
526
83.6
globlastp


2020
LNU74
blueberry|10v1|CF811622
3823
526
83.58
glotblastn


2021
LNU74
arabidopsis_lyrata|09v1|JGIAL012515
3824
526
82.8
globlastp


2022
LNU74
cucumber|09v1|CK700738
3825
526
82.8
globlastp


2023
LNU74
bean|gb167|CA897605
3826
526
82.8
globlastp


2024
LNU74
cichorium|gb166|EH700721
3827
526
82.8
globlastp


2025
LNU74
cichorium|gb171|EH700721
3827
526
82.8
globlastp


2026
LNU74
clover|gb162|BB922889
3828
526
82.8
globlastp


2027
LNU74
coffea|10v1|DV665694
3829
526
82.8
globlastp


2028
LNU74
coffea|gb157.2|DV665694
3829
526
82.8
globlastp


2029
LNU74
eucalyptus|gb166|CT981369
3830
526
82.8
globlastp


2030
LNU74
lettuce|gb157.2|DW120158
3831
526
82.8
globlastp


2031
LNU74
lettuce|gb157.2|DW148342
3831
526
82.8
globlastp


2032
LNU74
nuphar|gb166|CK768054
3832
526
82.8
globlastp


2033
LNU74
prunus|gb167|BU040832
3833
526
82.8
globlastp


2034
LNU74
safflower|gb162|EL375831
3834
526
82.8
globlastp


2035
LNU74
soybean|gb168|AW719570
3835
526
82.8
globlastp


2036
LNU74
triphysaria|gb164|EX989915
3836
526
82.8
globlastp


2037
LNU74
lettuce|10v1|DW045707
3831
526
82.8
globlastp


2038
LNU74
cucumber|09v1|AB008846
3837
526
82.1
globlastp


2039
LNU74
gerbera|09v1|AJ758629
3838
526
82.1
globlastp


2040
LNU74
liquorice|gb171|FS241908
3839
526
82.1
globlastp


2041
LNU74
rose|10v1|EC586454
3840
526
82.1
globlastp


2042
LNU74
lettuce|gb157.2|DW045707
3841
526
82.1
globlastp


2043
LNU74
lotus|09v1|LLAW163943
3842
526
82.1
globlastp


2044
LNU74
lotus|gb157.2|AW163943
3842
526
82.1
globlastp


2045
LNU74
medicago|09v1|AJ388670
3843
526
82.1
globlastp


2046
LNU74
medicago|gb157.2|AJ388670
3843
526
82.1
globlastp


2047
LNU74
melon|gb165|DV635132
3837
526
82.1
globlastp


2048
LNU74
sunflower|gb162|CD849558
3844
526
82.1
globlastp


2049
LNU74
sunflower|gb162|CD850836
3845
526
82.1
globlastp


2050
LNU74
sunflower|gb162|CD853111
3846
526
82.1
globlastp


2051
LNU74
tobacco|gb162|EB443461
3847
526
82.1
globlastp


2052
LNU74
petunia|gb171|DC240554
3848
526
81.48
glotblastn


2053
LNU74
antirrhinum|gb166|AJ789125
3849
526
81.34
glotblastn


2054
LNU74
liriodendron|gb166|CK767651
3850
526
81.34
glotblastn


2055
LNU74
cucumber|09v1|AM720896
3851
526
81.3
globlastp


2056
LNU74
gerbera|09v1|AJ750902
3852
526
81.3
globlastp


2057
LNU74
gerbera|09v1|AJ752958
3853
526
81.3
globlastp


2058
LNU74
centaurea|gb166|EH740419
3854
526
81.3
globlastp


2059
LNU74
cynara|gb167|GE588148
3855
526
81.3
globlastp


2060
LNU74
dandelion|gb161|DY837376
3856
526
81.3
globlastp


2061
LNU74
iceplant|gb164|BE033656
3857
526
81.3
globlastp


2062
LNU74
melon|gb165|AM720896
3851
526
81.3
globlastp


2063
LNU74
melon|gb165|EB714459
3858
526
81.3
globlastp


2064
LNU74
peanut|gb171|EE125965
3859
526
81.3
globlastp


2065
LNU74
zamia|gb166|CB095897
3860
526
81.3
globlastp


2066
LNU74
chickpea|09v2|GR398973
3861
526
80.6
glotblastn


2067
LNU74
ginseng|10v1|CN846065
3862
526
80.6
globlastp


2068
LNU74
pea|09v1|PSU78952
3863
526
80.6
glotblastn


2069
LNU74
artemisia|gb164|EY050285
3864
526
80.6
globlastp


2070
LNU74
artemisia|gb164|EY052651
3865
526
80.6
globlastp


2071
LNU74
centaurea|gb166|EH737655
3866
526
80.6
globlastp


2072
LNU74
medicago|09v1|AW686970
3867
526
80.6
globlastp


2073
LNU74
medicago|gb157.2|AW686970
3867
526
80.6
globlastp


2074
LNU74
peanut|gb167|EE123818
3868
526
80.6
globlastp


2075
LNU74
peanut|gb171|EE123818
3868
526
80.6
globlastp


2076
LNU74
peanut|gb167|EE125965
3869
526
80.6
globlastp


2077
LNU74
petunia|gb166|EB174480
3870
526
80.6
glotblastn


2078
LNU74
spruce|gb162|CO234251
3871
526
80.6
globlastp


2079
LNU74
sunflower|gb162|CD851355
3872
526
80.6
globlastp


2080
LNU74
tobacco|gb162|EB444354
3873
526
80.6
globlastp


2081
LNU74
petunia|gb171|EB174480
3874
526
80.4
globlastp


2082
LNU75
soybean|gb168|BQ453397
3875
527
96.5
globlastp


2083
LNU75
bean|gb167|CB540681
3876
527
94.43
glotblastn


2084
LNU75
castorbean|gb160|MDL29647M002010
3877
527
83.3
globlastp


2085
LNU75
poplar|10v1|CV248257
3878
527
83.2
globlastp


2086
LNU75
poplar|gb170|CV248257
3878
527
83.2
globlastp


2087
LNU75
poplar|10v1|BU817503
3879
527
82.1
globlastp


2088
LNU75
poplar|gb170|BU817503
3879
527
82.1
globlastp


2089
LNU75
cassava|09v1|DB930644
3880
527
80.47
glotblastn


2090
LNU75
castorbean|09v1|EG661615
3881
527
80.4
globlastp


2091
LNU75
lotus|09v1|CRPLJ030481
3882
527
80.2
globlastp


2092
LNU76
brachypodium|09v1|DV474282
3883
528
91.8
globlastp


2093
LNU76
oat|10v1|SRR020741S0039710
3884
528
91.3
globlastp


2094
LNU76
wheat|gb164|BE213625
3885
528
91.3
globlastp


2095
LNU76
barley|gb157.3|AJ234439
3886
528
91
globlastp


2096
LNU76
barley|gb157SOLEXA|AJ234439
3886
528
91
globlastp


2097
LNU76
sorghum|09v1|SB07G028080
3887
528
89.8
globlastp


2098
LNU76
sorghum|gb161.crp|AW283835
3887
528
89.8
globlastp


2099
LNU76
switchgrass|gb167|DN147961
3888
528
89.1
globlastp


2100
LNU76
maize|gb170|BI431326
3889
528
88.3
globlastp


2101
LNU76
ipomoea_nil|10v1|BJ557425
3890
528
87.8
globlastp


2102
LNU76
ipomoea|gb157.2|BM878831
3890
528
87.8
globlastp


2103
LNU76
tobacco|gb162|DV159853
3891
528
87.6
globlastp


2104
LNU76
eggplant|10v1|FS024720XX1
3892
528
87.3
globlastp


2105
LNU76
pepper|gb171|BM064103
3893
528
87.3
globlastp


2106
LNU76
chestnut|gb170|SRR006295S0084519
3894
528
87.3
globlastp


2107
LNU76
solanum_phureja|09v1|SPHAA824694
3895
528
87.1
globlastp


2108
LNU76
castorbean|09v1|EE254059
3896
528
87.1
globlastp


2109
LNU76
castorbean|gb160|EE254059
3896
528
87.1
globlastp


2110
LNU76
maize|gb170|SRR014549S0042197
3897
528
87.1
glotblastn


2111
LNU76
tomato|gb164|AA824694
3898
528
87.1
globlastp


2112
LNU76
nicotiana_benthamiana|gb162|
3899
528
87.06
glotblastn




CN741775






2113
LNU76
maize|gb170|BE640543
3900
528
86.85
glotblastn


2114
LNU76
cassava|09v1|CK641674
3901
528
86.8
globlastp


2115
LNU76
potato|gb157.2|BG096473
3902
528
86.8
globlastp


2116
LNU76
cleome_spinosa|10v1|SRR015531S0001775
3903
528
86.6
globlastp


2117
LNU76
aquilegia|10v1|DR924828
3904
528
86.6
globlastp


2118
LNU76
aquilegia|gb157.3|DR924828
3904
528
86.6
globlastp


2119
LNU76
medicago|09v1|AW698676
3905
528
86.6
globlastp


2120
LNU76
medicago|gb157.2|AW127555
3905
528
86.6
globlastp


2121
LNU76
melon|gb165|AF461048
3906
528
86.6
globlastp


2122
LNU76
potato|gb157.2|BE919491
3907
528
86.6
globlastp


2123
LNU76
pseudoroegneria|gb167|FF361372
3908
528
86.6
globlastp


2124
LNU76
potato|10v1|BG096473
3909
528
86.57
glotblastn


2125
LNU76
potato|10v1|BE919491
3910
528
86.32
glotblastn


2126
LNU76
cassava|09v1|CK641556
3911
528
86.3
globlastp


2127
LNU76
cucumber|09v1|AF461048
3912
528
86.3
globlastp


2128
LNU76
leymus|gb166|EG397002
3913
528
86.3
globlastp


2129
LNU76
melon|gb165|AY066012
3914
528
86.3
globlastp


2130
LNU76
triphysaria|gb164|EX982271
3915
528
86.3
globlastp


2131
LNU76
cleome_gynandra|10v1|SRR015532S0001935
3916
528
86.1
globlastp


2132
LNU76
cucumber|09v1|AY066012
3917
528
85.8
globlastp


2133
LNU76
apple|gb157.3|CN488454
3918
528
85.8
globlastp


2134
LNU76
apple|gb171|CN488454
3918
528
85.8
globlastp


2135
LNU76
apple|gb157.3|CN489537
3919
528
85.8
globlastp


2136
LNU76
apple|gb171|CN489537
3919
528
85.8
globlastp


2137
LNU76
b_oleracea|gb161|AM394032
3920
528
85.8
globlastp


2138
LNU76
canola|gb161|BQ704207
3920
528
85.8
globlastp


2139
LNU76
clover|gb162|BB932727
3921
528
85.8
globlastp


2140
LNU76
papaya|gb165|EX241410
3922
528
85.8
globlastp


2141
LNU76
peanut|gb167|ES752188
3923
528
85.8
globlastp


2142
LNU76
peanut|gb171|ES752188
3923
528
85.8
globlastp


2143
LNU76
chickpea|09v2|DY475133
3924
528
85.6
globlastp


2144
LNU76
b_juncea|gb164|EVGN00028714050635
3925
528
85.6
globlastp


2145
LNU76
b_rapa|gb162|CX272671
3926
528
85.6
globlastp


2146
LNU76
canola|10v1|CB686087
3926
528
85.6
globlastp


2147
LNU76
canola|gb161|CB686087
3926
528
85.6
globlastp


2148
LNU76
cichorium|gb171|EH672667
3927
528
85.6
globlastp


2149
LNU76
cotton|gb164|CO072567
3928
528
85.6
globlastp


2150
LNU76
ginger|gb164|DY344931
3929
528
85.6
globlastp


2151
LNU76
lettuce|gb157.2|DW047667
3930
528
85.6
globlastp


2152
LNU76
lettuce|gb157.2|DW101545
3930
528
85.6
globlastp


2153
LNU76
lettuce|gb157.2|DW146107
3930
528
85.6
globlastp


2154
LNU76
walnuts|gb166|EL891302
3931
528
85.6
globlastp


2155
LNU76
lettuce|10v1|DW047667
3930
528
85.6
globlastp


2156
LNU76
eucalyptus|gb166|CD668235
3932
528
85.57
glotblastn


2157
LNU76
lotus|09v1|AI967569
3933
528
85.3
globlastp


2158
LNU76
citrus|gb166|CF417173
3934
528
85.3
globlastp


2159
LNU76
cotton|gb164|CA993018
3935
528
85.3
globlastp


2160
LNU76
sunflower|gb162|CD845667
3936
528
85.3
globlastp


2161
LNU76
thellungiella|gb167|DN772880
3937
528
85.3
globlastp


2162
LNU76
artemisia|gb164|EY032115
3938
528
85.1
globlastp


2163
LNU76
b_rapa|gb162|BG544512
3939
528
85.1
globlastp


2164
LNU76
canola|10v1|BQ704676
3940
528
85.1
globlastp


2165
LNU76
canola|gb161|CX191423
3940
528
85.1
globlastp


2166
LNU76
poplar|10v1|BI068844
3941
528
85.1
globlastp


2167
LNU76
poplar|gb170|BI068844
3942
528
85.1
globlastp


2168
LNU76
poplar|10v1|BI069243
3943
528
85.1
globlastp


2169
LNU76
poplar|gb170|BI069243
3943
528
85.1
globlastp


2170
LNU76
radish|gb164|EV525299
3944
528
85.1
globlastp


2171
LNU76
banana|gb167|DN238082
3945
528
85.07
glotblastn


2172
LNU76
cichorium|gb166|EH672667
3946
528
85.07
glotblastn


2173
LNU76
b_oleracea|gb161|AM385579
3947
528
84.8
globlastp


2174
LNU76
coffea|10v1|DV678645
3948
528
84.8
globlastp


2175
LNU76
coffea|gb157.2|DV678645
3948
528
84.8
globlastp


2176
LNU76
cowpea|gb166|FC456675
3949
528
84.8
globlastp


2177
LNU76
cleome_gynandra|10v1|SRR015532S0005303
3950
528
84.6
globlastp


2178
LNU76
prunus|gb167|BU039541
3951
528
84.6
globlastp


2179
LNU76
strawberry|gb164|DY667189
3952
528
84.6
globlastp


2180
LNU76
ginger|gb164|DY344881
3953
528
84.3
globlastp


2181
LNU76
iceplant|gb164|AA842895
3954
528
84.3
globlastp


2182
LNU76
soybean|gb168|AI967569
3955
528
84.3
globlastp


2183
LNU76
arabidopsis|gb165|AT2G13360
3956
528
84.1
globlastp


2184
LNU76
bean|gb167|GFXEU018611X1
3957
528
84.1
globlastp


2185
LNU76
safflower|gb162|EL384125
3958
528
84.08
glotblastn


2186
LNU76
arabidopsis_lyrata|09v1|JGIAL011701
3959
528
83.8
globlastp


2187
LNU76
soybean|gb168|AW719840
3960
528
83.3
globlastp


2188
LNU76
lotus|09v1|AW719840
3961
528
82.8
globlastp


2189
LNU76
lotus|gb157.2|AW719840
3962
528
82.6
globlastp


2190
LNU76
grape|gb160|BM436787
3963
528
82.3
globlastp


2191
LNU76
spruce|gb162|CO230132
3964
528
81.1
globlastp


2192
LNU76
centaurea|gb166|EL931044
3965
528
81.09
glotblastn


2193
LNU76
pine|10v1|CF476545
3966
528
80.4
globlastp


2194
LNU76
pine|gb157.2|CF476545
3966
528
80.4
globlastp


2195
LNU79
cacao|gb167|CU499480
3967
529
82.8
globlastp


2196
LNU83
soybean|gb168|BI974032
3968
532
80.5
globlastp


2197
LNU85
sugarcane|10v1|BQ529864
3969
534
98.3
globlastp


2198
LNU85
sugarcane|gb157.3|BQ529864
3969
534
98.3
globlastp


2199
LNU85
maize|gb170|AI941787
3970
534
94.1
globlastp


2200
LNU85
switchgrass|gb167|DN152416
3971
534
91.4
globlastp


2201
LNU85
millet|09v1|EVO454PM001540
3972
534
89.9
globlastp


2202
LNU85
rice|gb170|OS05G48450
3973
534
88.8
globlastp


2203
LNU85
barley|gb157.3|BI954412
3974
534
86.6
globlastp


2204
LNU85
barley|gb157SOLEXA|BI954412
3974
534
86.6
globlastp


2205
LNU85
brachypodium|09v1|GT758786
3975
534
86.6
globlastp


2206
LNU85
wheat|gb164|BE427293
3976
534
84.9
globlastp


2207
LNU85
rice|gb170|OS10G40200
3977
534
81.7
globlastp


2208
LNU85
brachypodium|gb169|BE402800
3978
534
81.4
globlastp


2209
LNU85
switchgrass|gb167|FE658951
3979
534
80.6
globlastp


2210
LNU85
brachypodium|09v1|SRR031795S0009845
3980
534
80
globlastp


2211
LNU86
sorghum|09v1|SB09G029470
3981
535
85.4
globlastp


2212
LNU86
sorghum|gb161.crp|AI941972
3981
535
85.4
globlastp


2213
LNU89
wheat|gb164|BG606995
3982
537
98.7
globlastp


2214
LNU89
rice|gb170|OS12G16350
3983
537
82.4
globlastp


2215
LNU94
solanum_phureja|09v1|SPHAW621975
3984
538
96.4
globlastp


2216
LNU94
solanum_phureja|09v1|SPHDV105556
3985
538
94.7
globlastp


2217
LNU94
potato|10v1|X98891
3986
538
93.8
globlastp


2218
LNU94
potato|gb157.2|X98891
3986
538
93.8
globlastp


2219
LNU94
pepper|gb171|EF091665
3987
538
90.5
globlastp


2220
LNU94
tobacco|gb162|AB042951
3988
538
88.4
globlastp


2221
LNU94
pepper|gb157.2|EF091665
3989
538
87.3
globlastp


2222
LNU94
cichorium|gb171|DT213190
3990
538
81.44
glotblastn


2223
LNU94
cichorium|gb166|DT213190
3991
538
80.49
glotblastn


2224
LNU94
medicago|09v1|MTAF000354
3992
538
80.37
glotblastn


2225
LNU94
medicago|gb157.2|MTAF000354
3992
538
80.37
glotblastn


2226
LNU94
solanum_phureja|09v1|SPHY16125
3993
538
80.34
glotblastn


2227
LNU94
potato|10v1|AF156695
3993
538
80.34
glotblastn


2228
LNU94
potato|gb157.2|AF156695
3993
538
80.34
glotblastn


2229
LNU94
lettuce|10v1|DW051651
3994
538
80.3
glotblastn


2230
LNU94
lettuce|gb157.2|DW051651
3994
538
80.3
glotblastn


2231
LNU94
medicago|09v1|AW329601
3995
538
80.19
glotblastn


2232
LNU94
medicago|09v1|MTAF000355
3996
538
80.15
glotblastn


2233
LNU94
medicago|gb157.2|MTAF000355
3996
538
80.15
glotblastn


2234
LNU95
bean|gb167|CV543264
3997
539
84.1
globlastp


2235
LNU95
cowpea|gb166|FF384522
3998
539
82.2
globlastp


2236
LNU100
cotton|gb164|BG440037
3999
542
91.97
glotblastn


2237
LNU100
strawberry|gb164|EX657249
4000
542
82.95
glotblastn


2238
LNU100
cassava|09v1|DB921063
4001
542
82.9
globlastp


2239
LNU100
poplar|gb170|BI069411
4002
542
82.3
globlastp


2240
LNU100
poplar|gb170|BI131061
4003
542
82.1
globlastp


2241
LNU100
chestnut|gb170|SRR006295S0057433
4004
542
81.97
glotblastn


2242
LNU100
citrus|gb166|BQ624837
4005
542
81.89
glotblastn


2243
LNU100
poplar|10v1|BI131061
4006
542
81.3
globlastp


2244
LNU100
cowpea|gb166|FF394721
4007
542
80.25
glotblastn


2245
LNU100
soybean|gb168|AW163881
4008
542
80.25
glotblastn


2246
LNU100
cucumber|09v1|CK756513
4009
542
80.2
globlastp


2247
LNU100
bean|gb167|CA901142
4010
542
80.2
globlastp


2248
LNU100
medicago|09v1|AL369478
4011
542
80.04
glotblastn


2249
LNU100
medicago|09v1|AW691643
4012
542
80
globlastp


2250
LNU104
soybean|gb168|BG647792
4013
544
95.5
globlastp


2251
LNU104
cowpea|gb166|FF382988
4014
544
89.8
globlastp


2252
LNU104
medicago|09v1|BG647792
4015
544
82.8
globlastp


2253
LNU104
medicago|gb157.2|BG647792
4016
544
82
glotblastn


2254
LNU105
leymus|gb166|EG396306
4017
545
95.8
globlastp


2254
LNU72
leymus|gb166|EG396306
4017
725
94.2
globlastp


2255
LNU105
pseudoroegneria|gb167|FF352468
4018
545
94.2
globlastp


2255
LNU72
pseudoroegneria|gb167|FF352468
4018
725
92.7
globlastp


2256
LNU105
oat|10v1|CN819266
4019
545
88.8
globlastp


2256
LNU72
oat|10v1|CN819266
4019
725
88.4
globlastp


2257
LNU106
wheat|gb164|BE426509
4020
546
98.2
globlastp


2258
LNU106
barley|gb157.3|AJ234436
4021
546
91.9
globlastp


2259
LNU106
barley|gb157SOLEXA|AJ234436
4021
546
91.9
globlastp


2260
LNU107
brachypodium|09v1|SRR031795S0019801
4022
547
83.9
globlastp


2261
LNU107
brachypodium|gb169|BE637729
4022
547
83.9
globlastp


2262
LNU107
wheat|gb164|BE637729
4023
547
82.89
glotblastn


2263
LNU109
sorghum|09v1|SB10G000960
4024
548
85.6
globlastp


2264
LNU109
sorghum|gb161.crp|AW286123
4024
548
85.6
globlastp


2265
LNU109
brachypodium|09v1|GT863831
4025
548
85.2
globlastp


2266
LNU109
brachypodium|gb169|BE443129
4025
548
85.2
globlastp


2267
LNU109
pseudoroegneria|gb167|FF364297
4026
548
81.3
globlastp


2268
LNU115
sugarcane|10v1|CA067152
4027
553
84.3
globlastp


2269
LNU115
sugarcane|gb157.3|CA067152
4028
553
84.2
globlastp


2270
LNU115
sorghum|09v1|SB07G004420
4029
553
84.1
globlastp


2271
LNU115
maize|gb170|AW067055
4030
553
83.9
globlastp


2272
LNU115
brachypodium|09v1|TMPLOS03G48850T1
4031
553
80.09
glotblastn


2273
LNU115
rice|gb170|OS03G48850
4031
553
80.09
glotblastn


2274
LNU116
sorghum|09v1|SB01G021870
4032
554
80.2
globlastp


2275
LNU121
sorghum|gb161.crp|CF060003
4033
559
80.7
globlastp


2276
LNU121
brachypodium|gb169|BE601610
4034
559
80.39
glotblastn


2277
LNU123
arabidopsis_lyrata|09v1|GFXDQ132362X1
4035
561
98.5
globlastp


2278
LNU124
arabidopsis_lyrata|09v1|JGIAL029920
4036
562
99.3
globlastp


2279
LNU124
radish|gb164|FD967528
4037
562
83.03
glotblastn


2280
LNU124
citrus|gb166|CV885509
4038
562
81.08
glotblastn


2281
LNU124
poplar|10v1|CX176510
4039
562
80.72
glotblastn


2282
LNU124
poplar|gb170|CX176510
4039
562
80.72
glotblastn


2283
LNU126
arabidopsis_lyrata|09v1|JGIAL030477
4040
563
94.2
globlastp


2284
LNU126
canola|10v1|EE444561
4041
563
83.6
globlastp


2285
LNU126
b_rapa|gb162|DN964586
4042
563
83.6
glotblastn


2286
LNU126
canola|gb161|EE444561
4043
563
83.6
glotblastn


2287
LNU126
radish|gb164|EX747028
4044
563
81.94
glotblastn


2288
LNU126
b_juncea|gb164|EVGN00669227830274P1
4045
563
80.5
globlastp


2289
LNU127
arabidopsis_lyrata|09v1|JGIAL030813
4046
564
91.4
globlastp


2290
LNU127
thellungiella|gb167|DN772829
4047
564
81.2
globlastp


2291
LNU128
arabidopsis_lyrata|09v1|JGIAL002292
4048
565
98.8
globlastp


2292
LNU130
arabidopsis_lyrata|09v1|JGIAL007931
4049
567
86.6
globlastp


2293
LNU131
arabidopsis_lyrata|09v1|JGIAL015515
4050
568
92.9
globlastp


2294
LNU131
b_oleracea|gb161|DY026439
4051
568
82.7
globlastp


2295
LNU131
canola|gb161|DY023887
4052
568
82.33
glotblastn


2296
LNU131
canola|10v1|ES956350
4053
568
81.95
glotblastn


2297
LNU131
canola|10v1|DY023887
4054
568
80.6
glotblastn


2298
LNU131
radish|gb164|EW731027
4055
568
80.37
glotblastn


2299
LNU131
radish|gb164|EV546624
4056
568
80
glotblastn


2300
LNU132
arabidopsis_lyrata|09v1|JGIAL007040
4057
569
94.5
globlastp


2301
LNU133
arabidopsis_lyrata|09v1|JGIAL007458
4058
570
95.9
globlastp


2302
LNU133
canola|10v1|EE426894
4059
570
87.8
globlastp


2303
LNU133
canola|gb161|CX193266
4059
570
87.8
globlastp


2304
LNU133
b_rapa|gb162|CV434022
4060
570
86.7
globlastp


2305
LNU133
canola|gb161|CD830045
4061
570
86.7
globlastp


2306
LNU133
radish|gb164|EV527818
4062
570
86.7
globlastp


2307
LNU133
b_oleracea|gb161|AM057011
4063
570
85.7
globlastp


2308
LNU133
b_oleracea|gb161|ES948067
4064
570
85.7
globlastp


2309
LNU133
b_rapa|gb162|EE516981
4065
570
85.7
globlastp


2310
LNU133
canola|gb161|CD822076
4066
570
85.7
globlastp


2311
LNU133
canola|10v1|CX193266
4063
570
85.7
globlastp


2312
LNU133
canola|gb161|CX193799
4063
570
85.7
globlastp


2313
LNU133
radish|gb164|EW725954
4067
570
85.7
globlastp


2314
LNU133
radish|gb164|EW729334
4068
570
85.7
globlastp


2315
LNU133
radish|gb164|EX770388
4068
570
85.7
globlastp


2316
LNU133
radish|gb164|EX774120
4068
570
85.7
globlastp


2317
LNU133
b_juncea|gb164|EVGN00336011243437
4069
570
83.7
globlastp


2318
LNU133
b_juncea|gb164|EVGN00782708352054
4070
570
83.7
globlastp


2319
LNU133
b_oleracea|gb161|AM060067
4069
570
83.7
globlastp


2320
LNU133
b_rapa|gb162|CX266396
4069
570
83.7
globlastp


2321
LNU133
canola|10v1|BQ704992
4069
570
83.7
globlastp


2322
LNU133
canola|gb161|BQ704992
4069
570
83.7
globlastp


2323
LNU133
canola|10v1|H07830
4069
570
83.7
globlastp


2324
LNU133
canola|gb161|H07830
4069
570
83.7
globlastp


2325
LNU133
radish|gb164|EV527349
4069
570
83.7
globlastp


2326
LNU133
radish|gb164|EV535700
4069
570
83.7
globlastp


2327
LNU133
radish|gb164|EV568761
4069
570
83.7
globlastp


2328
LNU133
radish|gb164|EX747596
4069
570
83.7
globlastp


2329
LNU133
radish|gb164|FD538859
4071
570
82.7
globlastp


2330
LNU133
thellungiella|gb167|DN775946
4072
570
82.7
globlastp


2331
LNU133
b_rapa|gb162|EX025487
4073
570
82.47
glotblastn


2332
LNU133
radish|gb164|EV545406
4074
570
82.47
glotblastn


2333
LNU133
radish|gb164|FD981399
4075
570
81.44
glotblastn


2334
LNU134
arabidopsis_lyrata|09v1|JGIAL023230
4076
571
92.8
globlastp


2335
LNU136
arabidopsis_lyrata|09v1|JGIAL016753
4077
573
94.8
globlastp


2336
LNU136
canola|10v1|EV117254
4078
573
83.71
glotblastn


2337
LNU138
wheat|gb164|BE404910
4079
574
98.4
globlastp


2338
LNU138
pseudoroegneria|gb167|FF343940
4080
574
97.5
globlastp


2339
LNU138
wheat|gb164|BE405302
4081
574
97.2
globlastp


2340
LNU138
oat|10v1|GR335867
4082
574
91.5
globlastp


2341
LNU138
brachypodium|09v1|DV481179
4083
574
87.4
globlastp


2342
LNU138
brachypodium|gb169|BE404910
4084
574
83.91
glotblastn


2343
LNU138
rice|gb170|OS05G31750
4085
574
80.6
globlastp


2344
LNU140
canola|gb161|ES899922
4086
575
92.4
globlastp


2345
LNU141
barley|gb157.3|AV832506
4087
576
80.6
globlastp


2346
LNU141
barley|gb157SOLEXA|AV832506
4087
576
80.6
globlastp


2347
LNU142
wheat|gb164|BE429302
4088
577
88.1
globlastp


2348
LNU143
cotton|gb164|BQ411539
4089
578
83.63
glotblastn


2349
LNU147
heritiera|10v1|SRR005795S0005739
4090
580
83.8
globlastp


2350
LNU147
cacao|gb167|CU470446
4091
580
83.1
globlastp


2351
LNU148
soybean|gb168|CD399473
4092
581
87.7
globlastp


2352
LNU148
cowpea|gb166|FG822996
4093
581
81.5
globlastp


2353
LNU150
antirrhinum|gb166|AJ787462
4094
583
84.8
globlastp


2354
LNU153
switchgrass|gb167|FE648089
4095
584
91.8
globlastp


2355
LNU153
wheat|gb164|CA499728
4096
584
91.5
globlastp


2356
LNU153
sorghum|09v1|SB09G000910
4097
584
91.3
globlastp


2357
LNU153
sorghum|gb161.crp|AW564938
4097
584
91.3
globlastp


2358
LNU153
maize|gb170|AW787777
4098
584
89.5
globlastp


2359
LNU153
oat|10v1|GR331123
4099
584
89.08
glotblastn


2360
LNU153
switchgrass|gb167|FL747998
4100
584
85
globlastp


2361
LNU153
switchgrass|gb167|FL711487
4101
584
84.6
globlastp


2362
LNU153
brachypodium|09v1|DV482357
4102
584
84.4
globlastp


2363
LNU153
brachypodium|gb169|BE420118
4102
584
84.4
globlastp


2364
LNU153
rice|gb170|OS01G73970
4103
584
84.3
globlastp


2365
LNU153
barley|gb157SOLEXA|BI959596
4104
584
82.9
globlastp


2366
LNU153
sorghum|09v1|SB03G047280
4105
584
82.8
globlastp


2367
LNU153
sorghum|gb161.crp|BI139689
4105
584
82.8
globlastp


2368
LNU153
barley|gb157.3|BI959596
4106
584
82.6
globlastp


2369
LNU153
ginger|gb164|DY353392
4107
584
80.6
globlastp


2370
LNU157
soybean|gb168|AL365749
4108
587
96.5
globlastp


2371
LNU157
bean|gb167|CB540562
4109
587
93
globlastp


2372
LNU157
medicago|09v1|AW299124
4110
587
85.7
globlastp


2373
LNU161
soybean|gb168|CF808044
4111
589
88.8
globlastp


2374
LNU161
cowpea|gb166|FG846783
4112
589
80.8
globlastp


2375
LNU168
maize|gb170|AW927746
4113
590
97.2
globlastp


2376
LNU168
sugarcane|gb157.3|CA098262
4114
590
97.2
globlastp


2377
LNU168
sugarcane|10v1|BU102716
4114
590
97.2
globlastp


2378
LNU168
sugarcane|gb157.3|BU102716
4115
590
91.59
glotblastn


2379
LNU168
switchgrass|gb167|FL875098
4116
590
83.6
globlastp


2380
LNU168
switchgrass|gb167|DN151126
4117
590
82.7
globlastp


2381
LNU171
wheat|gb164|CK211341
4118
592
88.1
globlastp


2382
LNU171
wheat|gb164|CA712412
4119
592
85.3
globlastp


2383
LNU171
barley|gb157.3|BQ740207
4120
592
83.1
globlastp


2384
LNU171
barley|gb157SOLEXA|BQ740207
4120
592
83.1
globlastp


2385
LNU171
wheat|gb164|BE415592
4121
592
82.39
glotblastn


2386
LNU173
wheat|gb164|BE213263
4122
594
90.8
globlastp


2387
LNU173
wheat|gb164|BG607192
4123
594
90.2
globlastp


2388
LNU173
oat|10v1|Z48431
4124
594
80.3
globlastp


2389
LNU175
arabidopsis_lyrata|09v1|JGIAL025246
4125
595
92.5
globlastp


2390
LNU175
canola|10v1|CD820948
4126
595
85
globlastp


2391
LNU175
canola|gb161|CD820948
4126
595
85
globlastp


2392
LNU175
radish|gb164|EW731834
4126
595
85
globlastp


2393
LNU176
rice|gb170|OS07G23570
4127
596
85.7
globlastp


2394
LNU176
rice|gb170|OS07G44110
4128
596
81.6
globlastp


2395
LNU177
arabidopsis_lyrata|09v1|JGIAL006953
4129
597
95.2
globlastp


2396
LNU177
radish|gb164|EY932302
4130
597
81.3
globlastp


2397
LNU177
canola|10v1|BQ705039
4131
597
80.6
globlastp


2398
LNU177
canola|10v1|CN728969
4132
597
80.4
globlastp


2399
LNU177
canola|gb161|CN728969
4132
597
80.4
globlastp


2400
LNU177
b_oleracea|gb161|DY018716
4133
597
80.1
globlastp


2401
LNU177
b_rapa|gb162|EX061625
4134
597
80.1
globlastp


2402
LNU177
canola|gb161|BQ705039
4135
597
80.1
globlastp


2403
LNU178
arabidopsis_lyrata|09v1|JGIAL001921
4136
598
97.6
globlastp


2404
LNU178
canola|10v1|EE542748
4137
598
86.06
glotblastn


2405
LNU178
b_rapa|gb162|DN960788
4138
598
84.3
globlastp


2406
LNU178
canola|gb161|EL592266
4139
598
84.3
globlastp


2407
LNU178
radish|gb164|EV566211
4140
598
84.06
glotblastn


2408
LNU178
canola|10v1|EL592266
4141
598
83.8
globlastp


2409
LNU178
b_rapa|gb162|DN961047
4142
598
83.3
globlastp


2410
LNU178
radish|gb164|EX897481
4143
598
83.1
globlastp


2411
LNU180
arabidopsis_lyrata|09v1|JGIAL007244
4144
600
87.8
globlastp


2412
LNU181
arabidopsis_lyrata|09v1|JGIAL019086
4145
601
97.3
globlastp


2413
LNU181
arabidopsis|gb165|AT1G10490
4146
601
84.1
globlastp


2414
LNU181
arabidopsis_lyrata|09v1|JGIAL001043
4147
601
82.6
globlastp


2415
LNU182
arabidopsis_lyrata|09v1|JGIAL009295
4148
602
97.4
globlastp


2416
LNU182
canola|10v1|CD838490
4149
602
89
globlastp


2417
LNU182
canola|gb161|CD838490
4149
602
89
globlastp


2418
LNU182
thellungiella|gb167|BY804904
4150
602
87.7
globlastp


2419
LNU182
radish|gb164|EX905824
4151
602
87.1
globlastp


2420
LNU182
canola|10v1|ES915047
4152
602
87
globlastp


2421
LNU182
canola|gb161|ES915047
4152
602
87
globlastp


2422
LNU183
arabidopsis_lyrata|09v1|JGIAL006103
4153
603
96.2
globlastp


2423
LNU184
arabidopsis_lyrata|09v1|JGIAL018440
4154
604
88.4
globlastp


2424
LNU185
arabidopsis_lyrata|09v1|JGIAL019672
4155
605
91.3
globlastp


2425
LNU185
thellungiella|gb167|BM985521
4156
605
80
globlastp


2426
LNU186
arabidopsis_lyrata|09v1|BQ834224
4157
606
98.9
globlastp


2427
LNU186
canola|10v1|EE466406
4158
606
96.6
globlastp


2428
LNU186
b_rapa|gb162|EX032398
4159
606
96.1
globlastp


2429
LNU186
canola|gb161|EE466406
4160
606
96.1
globlastp


2430
LNU186
canola|10v1|CN730049
4161
606
95
globlastp


2431
LNU186
canola|gb161|CN730049
4161
606
95
globlastp


2432
LNU186
radish|gb164|EW725004
4162
606
94.4
globlastp


2433
LNU186
radish|gb164|EX746993
4162
606
94.4
globlastp


2434
LNU186
radish|gb164|EX755385
4162
606
94.4
globlastp


2435
LNU186
radish|gb164|EX763493
4163
606
93.9
globlastp


2436
LNU186
b_rapa|gb162|L38047
4164
606
93.3
globlastp


2437
LNU186
radish|gb164|EY911865
4165
606
93.3
globlastp


2438
LNU186
cleome_gynandra|10v1|SRR015532S0005426
4166
606
88.3
globlastp


2439
LNU186
cleome_spinosa|10v1|GR931536
4167
606
88.3
globlastp


2440
LNU186
cotton|gb164|BQ403404
4168
606
81
globlastp


2441
LNU186
cacao|gb167|CU485964
4169
606
80.4
globlastp


2442
LNU186
cotton|gb164|AI728654
4170
606
80.4
globlastp


2443
LNU187
arabidopsis_lyrata|09v1|JGIAL019765
4171
607
92.4
globlastp


2444
LNU188
soybean|gb168|BE661293
4172
608
90.9
globlastp


2445
LNU188
bean|gb167|FE696342
4173
608
80.24
glotblastn


2446
LNU190
b_oleracea|gb161|AM388062
4174
610
96.7
globlastp


2447
LNU190
canola|gb161|EV105578
4175
610
94.8
globlastp


2448
LNU190
radish|gb164|EV544043
4176
610
90.2
globlastp


2449
LNU192
sorghum|09v1|SB09G029740
4177
612
92.9
globlastp


2450
LNU192
brachypodium|gb169|BE406170
4178
612
90
globlastp


2451
LNU192
brachypodium|09v1|GT839119
4179
612
85.9
globlastp


2452
LNU192
sorghum|gb161.crp|AW747561
4180
612
82.5
globlastp


2453
LNU200
potato|10v1|BQ513965
4181
615
95.9
globlastp


2454
LNU200
potato|gb157.2|BQ513965
4182
615
95.3
globlastp


2455
LNU200
solanum_phureja|09v1|SPHBG791292
4183
615
93.8
globlastp


2456
LNU200
eggplant|10v1|FS057436
4184
615
92.1
globlastp


2457
LNU200
tobacco|gb162|DW002634
4185
615
87.7
globlastp


2458
LNU206
arabidopsis_lyrata|09v1|JGIAL014285
4186
617
93.3
globlastp


2459
LNU207
arabidopsis_lyrata|09v1|JGIAL007246
4187
618
94.9
globlastp


2460
LNU207
canola|10v1|H07749
4188
618
84.8
globlastp


2461
LNU207
canola|gb161|H07749
4188
618
84.8
globlastp


2462
LNU210
arabidopsis_lyrata|09v1|JGIAL019410
4189
619
94.1
globlastp


2463
LNU210
canola|10v1|EG019929
4190
619
85.86
glotblastn


2464
LNU210
canola|gb161|EG019929
4191
619
85.6
globlastp


2465
LNU210
radish|gb164|EW715949
4192
619
81.4
globlastp


2466
LNU211
arabidopsis_lyrata|09v1|JGIAL020302
4193
620
97.5
globlastp


2467
LNU211
thellungiella|gb167|BY811489
4194
620
96.7
globlastp


2468
LNU211
canola|10v1|EE452044
4195
620
90.2
globlastp


2469
LNU211
canola|10v1|EE465088
4196
620
90.2
globlastp


2470
LNU211
canola|gb161|EE465088
4196
620
90.2
globlastp


2471
LNU211
radish|gb164|EX746074
4195
620
90.2
globlastp


2472
LNU211
b_rapa|gb162|CV545795
4197
620
89.4
globlastp


2473
LNU211
canola|10v1|ES923006
4197
620
89.4
globlastp


2474
LNU211
canola|gb161|EE452044
4197
620
89.4
globlastp


2475
LNU211
canola|10v1|EE429968
4198
620
88.5
globlastp


2476
LNU211
canola|gb161|EE429968
4198
620
88.5
globlastp


2477
LNU211
b_rapa|gb162|BG544555
4199
620
86.89
glotblastn


2478
LNU211
b_rapa|gb162|CX269583
4200
620
86.89
glotblastn


2479
LNU214
canola|10v1|DV643275
4201
623
83
globlastp


2480
LNU214
canola|gb161|DV643275
4201
623
83
globlastp


2481
LNU214
b_oleracea|gb161|AM057785
4202
623
80.7
globlastp


2482
LNU215
arabidopsis_lyrata|09v1|JGIAL000823
4203
624
93.9
globlastp


2483
LNU215
canola|10v1|CD837114
4204
624
81.3
globlastp


2484
LNU215
radish|gb164|EX902620
4205
624
80.1
globlastp


2485
LNU216
rice|gb170|OS12G05440
4206
625
85.6
globlastp


2486
LNU216
maize|gb170|LLCF001713
4207
625
81.4
globlastp


2487
LNU216
sorghum|09v1|SB05G003100
4208
625
80.27
glotblastn


2488
LNU216
sorghum|gb161.crp|BG048909
4208
625
80.27
glotblastn


2489
LNU216
sorghum|09v1|SB08G003110
4209
625
80.1
globlastp


2490
LNU216
sorghum|gb161.crp|BE918845
4209
625
80.1
globlastp


2491
LNU216
maize|gb170|CA404041
4210
625
80
globlastp


2492
LNU218
arabidopsis_lyrata|09v1|JGIAL027135
4211
627
95.8
globlastp


2493
LNU218
canola|gb161|CD836926
4212
627
91.7
globlastp


2494
LNU218
radish|gb164|EW725001
4213
627
89.6
globlastp


2495
LNU218
castorbean|09v1|XM002514040
4214
627
81.35
glotblastn


2496
LNU218
castorbean|gb160|MDL29912M005300
4214
627
81.35
glotblastn


2497
LNU218
grape|gb160|BQ792882
4215
627
80.68
glotblastn


2498
LNU218
citrus|gb166|CN191381
4216
627
80.58
glotblastn


2499
LNU218
cassava|09v1|DB926789
4217
627
80.57
glotblastn


2500
LNU218
poplar|10v1|CV236445
4218
627
80.3
globlastp


2501
LNU218
poplar|gb170|CV236445
4218
627
80.3
globlastp


2502
LNU218
cucumber|09v1|GD175338
4219
627
80.2
globlastp


2503
LNU219
arabidopsis_lyrata|09v1|BQ834518
4220
628
99
globlastp


2504
LNU219
aquilegia|10v1|DR932989
4221
628
95.9
globlastp


2505
LNU219
canola|10v1|CD815027
4222
628
94.6
globlastp


2506
LNU219
canola|gb161|CD815027
4222
628
94.6
globlastp


2507
LNU219
radish|gb164|EW714771
4223
628
94.4
globlastp


2508
LNU219
b_rapa|gb162|EX020921
4224
628
94.1
globlastp


2509
LNU219
canola|10v1|CD836405
4225
628
93.9
globlastp


2510
LNU219
canola|gb161|CD836405
4225
628
93.9
globlastp


2511
LNU219
radish|gb164|EV539520
4226
628
92.6
globlastp


2512
LNU219
cleome_gynandra|10v1|SRR015532S0004520
4227
628
88.3
globlastp


2513
LNU219
cleome_spinosa|10v1|GR935537
4228
628
85.9
globlastp


2514
LNU219
b_rapa|gb162|BG544276
4229
628
84.7
globlastp


2515
LNU219
b_oleracea|gb161|AM385469
4230
628
84.4
globlastp


2516
LNU219
canola|gb161|CD818834
4231
628
84.4
globlastp


2517
LNU219
canola|gb161|T18344
4230
628
84.4
globlastp


2518
LNU219
canola|10v1|CD818834
4231
628
84.4
globlastp


2519
LNU219
radish|gb164|EV527315
4232
628
83.9
globlastp


2520
LNU219
arabidopsis_lyrata|09v1|JGIAL028336
4233
628
83.6
globlastp


2521
LNU219
arabidopsis|gb165|AT5G45280
4234
628
82.6
globlastp


2522
LNU222
brachypodium|09v1|DV476280
4235
630
91.4
globlastp


2522
LNU222_H6
brachypodium|09v1|DV476280
4235
680
91.4
globlastp


2523
LNU222
brachypodium|gb169|BE400657
4235
630
91.4
globlastp


2523
LNU222_H6
brachypodium|gb169|BE400657
4235
680
91.4
globlastp


2524
LNU222
rice|gb170|OS06G47890
4236
630
89.7
globlastp


2524
LNU222_H6
rice|gb170|OS06G47890
4236
680
94
globlastp


2525
LNU222
switchgrass|gb167|DN143448
4237
630
89.2
globlastp


2525
LNU222_H6
switchgrass|gb167|DN143448
4237
680
95.7
globlastp


2526
LNU222
sorghum|09v1|SB10G028340
680
630
88
globlastp


2527
LNU222
maize|gb170|AI734407
4238
630
87.3
globlastp


2527
LNU222_H6
maize|gb170|AI734407
4238
680
90.3
globlastp


2528
LNU222
maize|gb170|AI820142
4239
630
83.9
glotblastn


2528
LNU222_H6
maize|gb170|AI820142
4239
680
86.53
glotblastn


2529
LNU222
rice|gb170|OS02G05700
4240
630
80.16
glotblastn


2529
LNU222_H6
rice|gb170|OS02G05700
4240
680
80.2
globlastp


2530
LNU222
brachypodium|09v1|GT771721
4241
630
80.1
glotblastn


2530
LNU222_H6
brachypodium|09v1|GT771721
4241
680
80.8
globlastp


2531
LNU222
brachypodium|gb169|BE406203
4241
630
80.1
glotblastn


2531
LNU222_H6
brachypodium|gb169|BE406203
4241
680
80.8
globlastp


2532
LNU223
pseudoroegneria|gb167|FF350748
4242
631
87.3
globlastp


2533
LNU223
wheat|gb164|BF474058
4242
631
87.3
globlastp


2534
LNU223
maize|gb170|AI461537
4243
631
87.2
globlastp


2535
LNU223
oat|10v1|CN821280
4244
631
86.9
globlastp


2536
LNU223
sorghum|09v1|SB03G026180
4245
631
86.7
globlastp


2537
LNU223
sorghum|gb161.crp|Y14675
4245
631
86.7
globlastp


2538
LNU223
brachypodium|09v1|DV475278
4246
631
86.6
globlastp


2539
LNU223
brachypodium|gb169|BF474058
4246
631
86.6
globlastp


2540
LNU223
switchgrass|gb167|FL697289
4247
631
86.6
globlastp


2541
LNU223
barley|gb157SOLEXA|AL500275
4248
631
86.2
globlastp


2542
LNU223
leymus|gb166|EG378630
4249
631
86
globlastp


2543
LNU223
maize|gb170|AI740070
4250
631
85.6
globlastp


2544
LNU224
pseudoroegneria|gb167|FF346115
4251
632
97.1
globlastp


2545
LNU224
leymus|gb166|EG379498
4252
632
96.4
globlastp


2546
LNU224
wheat|gb164|BQ789174
4253
632
96.4
globlastp


2547
LNU224
wheat|gb164|BE401267
4254
632
95
globlastp


2548
LNU224
oat|10v1|GR322386
4255
632
88.7
globlastp


2549
LNU228
wheat|gb164|BE406956
4256
634
89.89
glotblastn


2550
LNU229
potato|10v1|BE922224
4257
635
94.6
globlastp


2551
LNU229
solanum_phureja|09v1|SPHAI484048
4258
635
94.4
globlastp


2552
LNU230
brachypodium|09v1|TMPLOS12G40300T1
636
636
100
globlastp


2553
LNU234
arabidopsis_lyrata|09v1|JGIAL002366
4259
638
94.4
globlastp


2554
LNU239
oat|10v1|GO581462
4260
641
94.8
globlastp


2555
LNU239
oat|10v1|GO587061
4261
641
94.8
globlastp


2556
LNU239
oat|10v1|GO587140
4260
641
94.8
globlastp


2557
LNU239
oat|10v1|GR361871
4260
641
94.8
globlastp


2558
LNU239
sorghum|09v1|SB10G001510
4262
641
94.8
globlastp


2559
LNU239
sorghum|gb161.crp|AW330931
4262
641
94.8
globlastp


2560
LNU239
switchgrass|gb167|FE639067
4263
641
94.8
globlastp


2561
LNU239
millet|09v1|EVO454PM053563
4264
641
93.1
glotblastn


2562
LNU239
oat|10v1|GR361767
4265
641
93.1
globlastp


2563
LNU239
sugarcane|10v1|BQ533648
4266
641
93.1
globlastp


2564
LNU239
sugarcane|10v1|CA103102
4266
641
93.1
globlastp


2565
LNU239
sugarcane|gb157.3|BQ533648
4266
641
93.1
globlastp


2566
LNU239
oat|10v1|GO581699
4267
641
91.4
globlastp


2567
LNU239
sorghum|09v1|SB10G002810
4268
641
91.4
globlastp


2568
LNU239
maize|gb170|AW330931
4269
641
91.4
globlastp


2569
LNU239
switchgrass|gb167|DN150836
4270
641
89.7
globlastp


2570
LNU239
wheat|gb164|BE413591
4271
641
89.66
glotblastn


2571
LNU239
wheat|gb164|BE443361
4272
641
89.66
glotblastn


2572
LNU239
wheat|gb164|CA613749
4273
641
89.66
glotblastn


2573
LNU239
rye|gb164|BE495091
4274
641
87.93
glotblastn


2574
LNU239
barley|gb157.3|AL501772
4275
641
87.9
globlastp


2575
LNU239
barley|gb157SOLEXA|AL501772
4275
641
87.9
globlastp


2576
LNU239
brachypodium|09v1|DV469471
4276
641
87.9
globlastp


2577
LNU239
brachypodium|gb169|BE413591
4276
641
87.9
globlastp


2578
LNU239
pseudoroegneria|gb167|FF348954
4277
641
87.9
globlastp


2579
LNU239
leymus|gb166|CD808544
4278
641
86.2
globlastp


2580
LNU239
maize|gb170|CD980142
4279
641
84.7
globlastp


2581
LNU239
banana|gb167|FL649074
4280
641
84.48
glotblastn


2582
LNU239
ginger|gb164|DY381315
4281
641
82.76
glotblastn


2583
LNU239
rice|gb170|OS06G03514
4282
641
81
globlastp


2584
LNU242
b_rapa|gb162|EX031422
4283
644
92.45
glotblastn


2585
LNU242
canola|10v1|DY002989
4284
644
88.68
glotblastn


2586
LNU242
radish|gb164|EV566917
4285
644
84.51
glotblastn


2587
LNU243
wheat|gb164|BE414904
4286
645
92.86
glotblastn


2588
LNU245
potato|gb157.2|CK262157
4287
647
93.7
globlastp


2589
LNU245
eggplant|10v1|FS013675
4288
647
89
globlastp


2590
LNU245
potato|gb157.2|BG590426
4289
647
88.3
globlastp


2591
LNU245
solanum_phureja|09v1|SPHAI486625
4290
647
88
globlastp


2592
LNU245
potato|10v1|BG590608
4291
647
81.3
globlastp


2593
LNU246
solanum_phureja|09v1|SPHAI896232
4292
648
97.1
globlastp


2594
LNU246
potato|10v1|BG599206
4293
648
96.8
globlastp


2595
LNU246
potato|gb157.2|BG599206
4293
648
96.8
globlastp


2596
LNU247
arabidopsis_lyrata|09v1|JGIAL021212
4294
649
93.3
globlastp


2597
LNU247
canola|10v1|CN827557
4295
649
87.6
globlastp


2598
LNU247
canola|gb161|CD826643
4296
649
86.83
glotblastn


2599
LNU249
arabidopsis_lyrata|09v1|JGIAL020153
4297
650
96.6
globlastp


2600
LNU251
arabidopsis_lyrata|09v1|JGIAL023392
4298
652
97
globlastp


2601
LNU253
bean|gb167|CB540282
4299
653
92.12
glotblastn


2602
LNU253
cowpea|gb166|FF387997
4300
653
89.16
glotblastn


2603
LNU253
peanut|gb167|EG029988
4301
653
81.37
glotblastn


2604
LNU253
peanut|gb171|EG029988
4301
653
81.37
glotblastn


2605
LNU253
soybean|gb168|BE657859
4302
653
81.3
globlastp


2606
LNU254
arabidopsis_lyrata|09v1|JGIAL004106
4303
654
99.8
globlastp


2607
LNU254
radish|gb164|EV526765
4304
654
97.3
globlastp


2608
LNU254
canola|10v1|CD833572
4305
654
95.8
globlastp


2609
LNU254
radish|gb164|EV526356
4306
654
95.4
globlastp


2610
LNU254
canola|gb161|CD833572
4307
654
93
globlastp


2611
LNU254
cotton|gb164|CO095176
4308
654
90.94
glotblastn


2612
LNU254
cassava|09v1|JGICASSAVA12947VALIDM1
4309
654
90.4
globlastp


2613
LNU254
cucumber|09v1|AM720434
4310
654
90.4
globlastp


2614
LNU254
tomato|09v1|BQ119293
4311
654
90.3
globlastp


2615
LNU254
poplar|10v1|BI131005
4312
654
90.2
globlastp


2616
LNU254
poplar|gb170|BI131005
4312
654
90.2
globlastp


2617
LNU254
solanum_phureja|09v1|SPHBQ119293
4313
654
89.9
globlastp


2618
LNU254
castorbean|09v1|XM002510240
4314
654
89.8
globlastp


2619
LNU254
castorbean|gb160|MDL30170M013940
4314
654
89.8
globlastp


2620
LNU254
soybean|gb168|AW693383
4315
654
89.8
globlastp


2621
LNU254
chestnut|gb170|SRR006295S0002298
4316
654
89.5
globlastp


2622
LNU254
cassava|09v1|JGICASSAVA24790VALIDM1
4317
654
89.1
globlastp


2623
LNU254
soybean|gb168|BE320506
4318
654
89
globlastp


2624
LNU254
medicago|09v1|AL365842
4319
654
88.9
globlastp


2625
LNU254
potato|10v1|BQ119293
4320
654
87.5
globlastp


2626
LNU254
potato|gb157.2|BQ119293
4320
654
87.5
globlastp


2627
LNU254
aquilegia|10v1|DR914712
4321
654
86.4
globlastp


2628
LNU254
monkeyflower|10v1|GO954748
4322
654
85.5
globlastp


2629
LNU255
arabidopsis_lyrata|09v1|JGIAL015618
4323
655
87.3
globlastp


2630
LNU256
arabidopsis|gb165|AT2G43910
4324
656
83.3
globlastp


2631
LNU256
canola|10v1|EG020127
4325
656
81.9
globlastp


2632
LNU256
b_rapa|gb162|CA992319
4326
656
81.5
globlastp


2633
LNU256
b_rapa|gb162|L46502
4327
656
80.6
globlastp


2634
LNU256
b_oleracea|gb161|AF387791
4328
656
80.2
globlastp


2635
LNU256
canola|10v1|CD812772
4329
656
80.2
globlastp


2636
LNU256
canola|gb161|CD812772
4329
656
80.2
globlastp


2637
LNU256
radish|gb164|EV524444
4330
656
80.2
globlastp


2638
LNU257
arabidopsis_lyrata|09v1|JGIAL009641
4331
657
98.2
globlastp


2639
LNU257
radish|gb164|EV543963
4332
657
94.3
globlastp


2640
LNU257
thellungiella|gb167|BY830471
4333
657
94
globlastp


2641
LNU258
arabidopsis_lyrata|09v1|JGIAL024223
4334
658
90.65
glotblastn


2642
LNU260
thellungiella|gb167|DN774718
4335
659
84.9
globlastp


2643
LNU260
b_juncea|gb164|EVGN00742808281526
4336
659
82.8
globlastp


2644
LNU260
radish|gb164|EV536406
4337
659
80.8
globlastp


2645
LNU260
b_oleracea|gb161|DY015251
4338
659
80.51
glotblastn


2646
LNU260
radish|gb164|EV566818
4339
659
80.43
glotblastn


2647
LNU260
b_rapa|gb162|EX108082
4340
659
80.3
globlastp


2648
LNU260
b_rapa|gb162|ES932807
4341
659
80.08
glotblastn


2649
LNU260
canola|10v1|CX194829
4341
659
80.08
glotblastn


2650
LNU260
canola|gb161|CX194829
4341
659
80.08
glotblastn


2651
LNU260
radish|gb164|EV538732
4342
659
80
globlastp


2652
LNU261
arabidopsis_lyrata|09v1|JGIAL029133
4343
660
95.2
globlastp


2653
LNU261
radish|gb164|EV565501
4344
660
82.9
globlastp


2654
LNU262
arabidopsis_lyrata|09v1|JGIAL029381
4345
661
95.2
globlastp


2655
LNU263
wheat|gb164|BE403303
4346
662
92.6
globlastp


2656
LNU263
wheat|gb164|BE405309
4347
662
92.6
globlastp


2657
LNU263
barley|gb157SOLEXA|BE412891
4348
662
91.2
globlastp


2658
LNU263
leymus|gb166|EG394979
4349
662
84.5
glotblastn


2659
LNU263
brachypodium|09v1|DV474340
4350
662
83.9
globlastp


2660
LNU263
brachypodium|gb169|BE445700
4350
662
83.9
globlastp


2661
LNU263
brachypodium|09v1|DV473838
4351
662
82.9
globlastp


2662
LNU263
brachypodium|gb169|DV473838
4351
662
82.9
globlastp


2663
LNU263
sorghum|09v1|SB02G040500
4352
662
80.4
globlastp


2664
LNU263
sorghum|gb161.crp|BM382553
4352
662
80.4
globlastp


2665
LNU263
sorghum|09v1|SB02G040510
4353
662
80.2
globlastp


2666
LNU263
sorghum|gb161.crp|BM661046
4353
662
80.2
globlastp


2667
LNU265
sorghum|09v1|SB09G000890
4354
663
92
globlastp


2668
LNU265
sorghum|gb161.crp|BE597117
4355
663
86.3
globlastp


2669
LNU266
sorghum|09v1|SB03G002900
4356
664
93.6
globlastp


2670
LNU266
sorghum|gb161.crp|AI987574
4357
664
87.1
globlastp


2671
LNU267
maize|gb170|LLDV514913
4358
665
85.7
globlastp


2672
LNU267
maize|gb170|AI637120
4359
665
84
globlastp


2673
LNU267
sugarcane|10v1|BQ535909
4360
665
82.6
globlastp


2674
LNU267
sorghum|09v1|SB04G022740
4361
665
82.2
globlastp


2675
LNU267
sorghum|gb161.crp|AW283751
4361
665
82.2
globlastp


2676
LNU267
switchgrass|gb167|FE640888
4362
665
82
globlastp


2677
LNU267
sugarcane|gb157.3|BQ535909
4363
665
81.1
globlastp


2678
LNU267
switchgrass|gb167|FE621258
4364
665
81.1
globlastp


2679
LNU268
sorghum|09v1|SB03G002890
4365
666
94.8
globlastp


2680
LNU268
sorghum|gb161.crp|BF656912
4365
666
94.8
globlastp


2681
LNU268
sugarcane|gb157.3|CA082317
4366
666
93.8
globlastp


2682
LNU268
maize|gb170|LLEE026298
4367
666
92.2
globlastp


2683
LNU268
switchgrass|gb167|DN141482
4368
666
91.7
globlastp


2684
LNU268
sugarcane|10v1|CA219295
4369
666
86.53
glotblastn


2685
LNU268
barley|gb157SOLEXA|BE060428
4370
666
85.5
globlastp


2686
LNU268
brachypodium|09v1|GT836297
4371
666
84.5
globlastp


2687
LNU271
brachypodium|09v1|GT783626
4372
667
90.2
globlastp


2688
LNU271
brachypodium|gb169|BF483222
4372
667
90.2
globlastp


2689
LNU271
wheat|gb164|BF483222
4373
667
90.2
globlastp


2690
LNU271
wheat|gb164|CA700889
4373
667
90.2
globlastp


2691
LNU271
sorghum|09v1|SB01G043280
4374
667
89
globlastp


2692
LNU271
sorghum|gb161.crp|CD210322
4374
667
89
globlastp


2693
LNU271
oat|10v1|GO592793
4375
667
87.9
globlastp


2694
LNU271
switchgrass|gb167|DN144438
4376
667
87.3
globlastp


2695
LNU271
maize|gb170|CD944785
4377
667
86.1
globlastp


2696
LNU271
maize|gb170|H35893
4378
667
85
globlastp


2697
LNU271
millet|09v1|EVO454PM060798
4379
667
84.1
globlastp


2698
LNU271
fescue|gb161|DT710736
4380
667
83.8
globlastp


2699
LNU274
rice|gb170|OS05G01750
4381
668
93
globlastp


2700
LNU275
brachypodium|09v1|SRR031797S0128470
4382
669
80.33
glotblastn


2701
LNU275
sorghum|09v1|SB02G030360
4383
669
80.3
globlastp


2702
LNU275
maize|gb170|DN213402
4384
669
80.1
globlastp


2703
LNU278
switchgrass|gb167|FL852997
4385
672
93
globlastp


2704
LNU278
millet|09v1|EVO454PM020322
4386
672
90.6
globlastp


2705
LNU278
brachypodium|09v1|GT841667
4387
672
88.1
globlastp


2706
LNU278
brachypodium|gb169|BG343026
4387
672
88.1
globlastp


2707
LNU278
leymus|gb166|EG395159
4388
672
87.9
globlastp


2708
LNU278
oat|10v1|GR322586
4389
672
87.4
globlastp


2709
LNU278
maize|gb170|DR964461
4390
672
86.4
globlastp


2710
LNU278
maize|gb170|DN217333
4391
672
83.1
globlastp


2711
LNU278
rice|gb170|OS05G03530
4392
672
83.05
glotblastn


2712
LNU278
sugarcane|gb157.3|CA139449
4393
672
81.5
globlastp


2713
LNU279
maize|gb170|AW126569
4394
673
92
globlastp


2714
LNU279
maize|gb170|DR823071
4395
673
91.7
globlastp


2715
LNU279
maize|gb170|CF014369
4396
673
87.1
globlastp


2716
LNU279
maize|gb170|CD961214
4397
673
84.8
globlastp


2717
LNU279
sorghum|09v1|SB02G037590
4398
673
84.7
globlastp


2718
LNU280
switchgrass|gb167|FL809443
4399
674
90.5
globlastp


2719
LNU280
maize|gb170|BM498380
4400
674
88.5
globlastp


2720
LNU280
rice|gb170|OS11G23790
4401
674
83.8
globlastp


2721
LNU280
brachypodium|09v1|DV474046
4402
674
83
globlastp


2722
LNU280
brachypodium|gb169|BF482671
4403
674
82.49
glotblastn


2723
LNU280
wheat|gb164|BF293467
4404
674
82.28
glotblastn


2724
LNU282
soybean|gb168|BQ630236
4405
675
96.9
globlastp


2725
LNU282
medicago|09v1|AW686001
4406
675
86.4
globlastp


2726
LNU282
lotus|09v1|AI967570
4407
675
81.5
globlastp


2727
LNU284
soybean|gb168|CF807230
4408
676
93
globlastp


2728
LNU284
soybean|gb168|BQ080894
4409
676
92.8
globlastp


2729
LNU284
soybean|gb168|AW351157
4410
676
82.7
globlastp


2730
LNU288
solanum_phureja|09v1|SPHBG134658
4411
678
97.7
globlastp


2731
LNU288
potato|10v1|BE920963
4412
678
96.7
globlastp


2732
LNU288
potato|gb157.2|BE920963
4412
678
96.7
globlastp


2733
LNU288
tobacco|gb162|AB014483
4413
678
85.8
globlastp


2734
LNU289
solanum_phureja|09v1|SPHBG123295
4414
679
97.2
globlastp


2735
LNU289
potato|gb157.2|BG592935
4415
679
96.5
globlastp


2736
LNU289
potato|10v1|BG592935
4416
679
95.1
globlastp


2737
LNU289
eggplant|10v1|FS024715
4417
679
93.6
globlastp


2738
LNU289
pepper|gb171|CK901930
4418
679
81.27
glotblastn


2739
LNU222_H6
sorghum|09v1|SB04G003660
4419
680
81.3
globlastp


2740
LNU222_H6
maize|gb170|AW928070
4420
680
81
globlastp


2741
LNU29
potato|10v1|BM405532
3260
681
89.42
glotblastn


2742
LNU35
rice|gb170|OS07G13590
4421
682
89.97
glotblastn


2743
LNU35
sorghum|09v1|SB02G007060
4422
682
89.62
glotblastn


2744
LNU35
sorghum|gb161.crp|AW067593
4422
682
89.62
glotblastn


2745
LNU35
maize|gb170|AI601000
4423
682
89.27
glotblastn


2746
LNU35
barley|gb157.3|AV833599
4424
682
89.1
globlastp


2747
LNU35
barley|gb157SOLEXA|AV833599
4425
682
89.1
globlastp


2748
LNU35
brachypodium|gb169|BE442655
4426
682
86.2
globlastp


2749
LNU55
soybean|gb168|SB2GWP093054
4427
684
88.1
globlastp


2750
LNU60
barley|gb157SOLEXA|BG368863
4428
686
92.82
glotblastn


2751
LNU60
barley|gb157.3|BG368863
4428
686
92.53
glotblastn


2752
LNU60
pseudoroegneria|gb167|FF360223
4429
686
81.6
globlastp


2753
LNU60
switchgrass|gb167|FL699261
4430
686
80.52
glotblastn


2754
LNU83
cowpea|gb166|FF386177
4431
687
89.08
glotblastn


2755
LNU83
peanut|gb171|EE124103
4432
687
80.28
glotblastn


2756
LNU89
pseudoroegneria|gb167|FF341285
4433
688
95.76
glotblastn


2757
LNU89
maize|gb170|AA979933
4434
688
80.08
glotblastn


2758
LNU115
rice|gb170|OS03G21740
4435
693
83.6
globlastp


2759
LNU115
wheat|gb164|BE498586
4436
693
80.1
glotblastn


2760
LNU170
arabidopsis_lyrata|09v1|TMPLAT5G40060T1
4437
697
100
glotblastn


2761
LNU192
sugarcane|gb157.3|BQ533786
4438
698
93.97
glotblastn


2762
LNU192
switchgrass|gb167|FL700814
4439
698
91.11
glotblastn


2763
LNU192
monkeyflower|10v1|GR013631
4440
698
86.67
glotblastn


2764
LNU192
poplar|gb170|BI138135
4441
698
82.54
glotblastn


2765
LNU192
poplar|10v1|BI138135
4442
698
82.22
glotblastn


2766
LNU192
poplar|10v1|XM002307099
4443
698
81.88
glotblastn


2767
LNU192
poplar|gb170|XM002307099
4444
698
81.88
glotblastn


2768
LNU192
cucumber|09v1|CV001584
4445
698
81.59
glotblastn


2769
LNU192
soybean|gb168|BQ252456
4446
698
81.27
glotblastn


2770
LNU192
castorbean|09v1|XM002532133
4447
698
80.95
glotblastn


2771
LNU192
medicago|09v1|AW689616
4448
698
80.95
glotblastn


2772
LNU192
soybean|gb168|BM523433
4449
698
80.95
glotblastn


2773
LNU229
potato|gb157.2|BE922224
4450
701
93.8
globlastp


2774
LNU242
arabidopsis_lyrata|09v1|JGIAL023703
4451
703
88.6
globlastp


2775
LNU265
switchgrass|gb167|FE626072
4452
705
86.1
globlastp


2776
LNU4
rye|gb164|BE494134
4453
708
95.5
globlastp


2777
LNU4
wheat|gb164|BE430340
4454
708
94.2
globlastp


2778
LNU4
wheat|gb164|BQ743944
4455
708
93.5
globlastp


2779
LNU4
wheat|gb164|CD935835
4456
708
92.9
globlastp


2780
LNU4
brachypodium|09v1|DV474688
4457
708
86.4
globlastp


2781
LNU4
brachypodium|gb169|BE494134
4457
708
86.4
globlastp


2782
LNU4
oat|10v1|GR330356
4458
708
84.4
globlastp


2783
LNU4
barley|gb157.3|BI955043
4459
708
84.1
globlastp


2784
LNU4
barley|gb157SOLEXA|BI955043
4459
708
84.1
globlastp


2785
LNU4
barley|gb157SOLEXA|BE519514
4460
708
81.1
globlastp


2786
LNU4
oat|10v1|GR364394
4461
708
80.5
globlastp


2787
LNU4
wheat|gb164|CK214316
4462
708
80.4
globlastp


2788
LNU28
oat|10v1|GR315782
4463
712
90.9
globlastp


2789
LNU28
sorghum|09v1|SB03G008060
4464
712
83.7
globlastp


2790
LNU28
sorghum|gb161.crp|AW283962
4464
712
83.7
globlastp


2791
LNU28
sugarcane|10v1|CA109293
4465
712
82
globlastp


2792
LNU28
rice|gb170|OS01G02870
4466
712
81.7
globlastp


2793
LNU28
sugarcane|gb157.3|CA109293
4467
712
81.5
globlastp


2794
LNU28
switchgrass|gb167|FE643547
4468
712
80.2
globlastp


2795
LNU35
brachypodium|09v1|DV488691
4469
714
85.8
globlastp


2796
LNU36
soybean|gb168|BG839931
4470
715
80.4
globlastp


2797
LNU54
soybean|gb168|BG839336
4471
717
94.9
globlastp


2798
LNU54
cowpea|gb166|FC457406
4472
717
85.5
globlastp


2799
LNU54
bean|gb167|CA905321
4473
717
85
globlastp


2800
LNU58
wheat|gb164|BE430622
4474
719
88.4
globlastp


2801
LNU58
wheat|gb164|CA622084
4475
719
82.8
globlastp


2802
LNU58
wheat|gb164|BF484060
4476
719
80.4
globlastp


2803
LNU64
wheat|gb164|AJ603788
4477
721
92.2
globlastp


2803
LNU98
wheat|gb164|AJ603788
4477
734
80.2
globlastp


2804
LNU64
millet|09v1|CD725273
4478
721
83.5
globlastp


2804
LNU98
millet|09v1|CD725273
4478
734
82.2
globlastp


2805
LNU64
wheat|gb164|BE591591
4479
721
82.6
globlastp


2805
LNU98
wheat|gb164|BE591591
4479
734
80.23
glotblastn


2806
LNU70
wheat|gb164|BE492123
4480
724
92.41
glotblastn


2807
LNU70
barley|gb157SOLEXA|AL506812
4481
724
92.1
globlastp


2808
LNU70
brachypodium|09v1|DV469959
4482
724
90.9
globlastp


2809
LNU70
sugarcane|10v1|BQ533718
4483
724
90.9
globlastp


2810
LNU70
maize|gb170|AA051893
4484
724
90.5
globlastp


2811
LNU70
switchgrass|gb167|FE616904
4485
724
90.3
globlastp


2812
LNU70
sorghum|09v1|SB02G000720
4486
724
90.2
globlastp


2813
LNU70
maize|gb170|LLBM501434
4487
724
89.9
globlastp


2814
LNU70
switchgrass|gb167|FE597592
4488
724
89.7
globlastp


2815
LNU70
rice|gb170|OS07G01020
4489
724
89.6
globlastp


2816
LNU70
rice|gb170|OS10G01080
4490
724
89.2
globlastp


2817
LNU70
oat|10v1|GR350932
4491
724
87.7
glotblastn


2818
LNU70
ginger|gb164|DY345087
4492
724
87.5
globlastp


2819
LNU70
ginger|gb164|DY345406
4493
724
87.5
globlastp


2820
LNU70
leymus|gb166|EG378516
4494
724
87.2
globlastp


2821
LNU70
brachypodium|09v1|SRR031795S0034512
4495
724
86.9
globlastp


2822
LNU70
barley|gb157SOLEXA|BE421769
4496
724
86.7
globlastp


2823
LNU70
antirrhinum|gb166|AJ794293
4497
724
86.2
globlastp


2824
LNU70
aquilegia|10v1|DR915720
4498
724
86.2
globlastp


2825
LNU70
orobanche|10v1|SRR023189S0008709
4499
724
86.2
globlastp


2826
LNU70
cleome_gynandra|10v1|SRR015532S0033509
4500
724
85.9
globlastp


2827
LNU70
wheat|gb164|BE216934
4501
724
85.8
globlastp


2828
LNU70
cotton|gb164|AI731099
4502
724
85.6
globlastp


2829
LNU70
cotton|gb164|AI727639
4503
724
85.3
globlastp


2830
LNU70
cassava|09v1|CK646456
4504
724
84.9
globlastp


2831
LNU70
cleome_gynandra|10v1|SRR015532S0002004
4505
724
84.9
globlastp


2832
LNU70
grape|gb160|BM436391
4506
724
84.9
globlastp


2833
LNU70
oat|10v1|GR314124
4507
724
84.9
globlastp


2834
LNU70
soybean|gb168|AL368316
4508
724
84.9
globlastp


2835
LNU70
artemisia|gb164|EY100674
4509
724
84.6
globlastp


2836
LNU70
monkeyflower|10v1|DV211139
4510
724
84.6
globlastp


2837
LNU70
poplar|10v1|BU821620
4511
724
84.6
globlastp


2838
LNU70
soybean|gb168|BG583573
4512
724
84.6
globlastp


2839
LNU70
cleome_gynandra|10v1|SRR015532S0001986
4513
724
84.4
globlastp


2840
LNU70
apple|gb171|CN581367
4514
724
84.3
globlastp


2841
LNU70
aquilegia|10v1|DR926939
4515
724
84.3
globlastp


2842
LNU70
bean|gb167|AY007525
4516
724
84.3
globlastp


2843
LNU70
cichorium|gb171|EH673869
4517
724
84.3
globlastp


2844
LNU70
citrus|gb166|CB291221
4518
724
84.3
globlastp


2845
LNU70
lettuce|10v1|DW065212
4517
724
84.3
globlastp


2846
LNU70
lettuce|10v1|DW074895
4517
724
84.3
globlastp


2847
LNU70
b_rapa|gb162|CV650466
4519
724
84
globlastp


2848
LNU70
canola|10v1|BQ704215
4519
724
84
globlastp


2849
LNU70
cowpea|gb166|FC459209
4520
724
84
globlastp


2850
LNU70
cucumber|09v1|BI740201
4521
724
84
globlastp


2851
LNU70
ipomoea_nil|10v1|BJ563728
4522
724
84
globlastp


2852
LNU70
pepper|gb171|BM059644
4523
724
84
globlastp


2853
LNU70
poplar|10v1|BI071183
4524
724
84
globlastp


2854
LNU70
potato|10v1|BG350133
4525
724
84
globlastp


2855
LNU70
solanum_phureja|09v1|SPHBG127432
4525
724
84
globlastp


2856
LNU70
sunflower|gb162|DY910980
4526
724
84
globlastp


2857
LNU70
thellungiella|gb167|DN773941
4527
724
84
globlastp


2858
LNU70
b_rapa|gb162|CV544377
4528
724
83.7
globlastp


2859
LNU70
canola|10v1|CX193292
4528
724
83.7
globlastp


2860
LNU70
canola|10v1|EE413831
4529
724
83.7
globlastp


2861
LNU70
canola|10v1|EE470024
4530
724
83.7
globlastp


2862
LNU70
chestnut|gb170|SRR006295S0008684
4531
724
83.7
globlastp


2863
LNU70
eggplant|10v1|FS022577
4532
724
83.7
globlastp


2864
LNU70
nicotiana_benthamiana|gb162|
4533
724
83.7
globlastp




CN744951






2865
LNU70
tobacco|gb162|GFXAY532656X1
4533
724
83.7
globlastp


2866
LNU70
tomato|09v1|BG127432
4534
724
83.7
globlastp


2867
LNU70
kiwi|gb166|FG409049
4535
724
83.4
globlastp


2868
LNU70
strawberry|gb164|CO379446
4536
724
83.4
globlastp


2869
LNU70
arabidopsis_lyrata|09v1|BQ834310
4537
724
83.3
globlastp


2870
LNU70
arabidopsis|gb165|AT5G01410
4537
724
83.3
globlastp


2871
LNU70
canola|10v1|CB686329
4538
724
83.3
globlastp


2872
LNU70
cassava|09v1|CK643506
4539
724
83.1
globlastp


2873
LNU70
canola|10v1|CB686238
4540
724
83
globlastp


2874
LNU70
clover|gb162|BB936964
4541
724
82.7
globlastp


2875
LNU70
lotus|09v1|DQ139264
4542
724
82.7
globlastp


2876
LNU70
citrus|gb166|CB292808
4543
724
82.4
globlastp


2877
LNU70
lettuce|10v1|DW090121
4544
724
82.4
globlastp


2878
LNU70
papaya|gb165|EX254443
4545
724
82.4
globlastp


2879
LNU70
strawberry|gb164|EX658116
4546
724
82.2
globlastp


2880
LNU70
castorbean|09v1|EG661356
4547
724
82.11
glotblastn


2881
LNU70
cotton|gb164|DT555568
4548
724
82.1
globlastp


2882
LNU70
cryptomeria|gb166|BP175070
4549
724
82.1
globlastp


2883
LNU70
spruce|gb162|CO222779
4550
724
82.1
globlastp


2884
LNU70
spruce|gb162|CO478481
4551
724
82.1
globlastp


2885
LNU70
cynara|gb167|GE587800
4552
724
82.05
glotblastn


2886
LNU70
pine|10v1|CO170442
4553
724
81.7
globlastp


2887
LNU70
arabidopsis_lyrata|09v1|JGIAL015042
4554
724
81.4
globlastp


2888
LNU70
arabidopsis|gb165|AT2G38230
4555
724
81.4
globlastp


2889
LNU70
fern|gb171|BP912037
4556
724
81.4
globlastp


2890
LNU70
pine|10v1|CF470198
4557
724
81.4
globlastp


2891
LNU70
radish|gb164|EY902227
4558
724
81.4
globlastp


2892
LNU70
medicago|09v1|AW695944
4559
724
81.3
globlastp


2893
LNU70
b_oleracea|gb161|AM385028
4560
724
80.9
globlastp


2894
LNU70
cucumber|09v1|AM723122
4561
724
80.6
globlastp


2895
LNU70
spikemoss|gb165|FE429245
4562
724
80.13
glotblastn


2896
LNU70
spikemoss|gb165|FE451328
4562
724
80.13
glotblastn


2897
LNU74
switchgrass|gb167|FE639952
4563
726
86.7
globlastp


2898
LNU74
millet|09v1|EVO454PM015812
4564
726
86.57
glotblastn


2899
LNU74
switchgrass|gb167|FE657764
4565
726
85.9
globlastp


2900
LNU74
rice|gb170|OS04G43540
4566
726
85.8
globlastp


2901
LNU74
brachypodium|09v1|DV476963
4567
726
85.2
globlastp


2902
LNU74
maize|gb170|AI621531
4568
726
85.2
globlastp


2903
LNU74
maize|gb170|LLBE128837
4568
726
85.2
globlastp


2904
LNU74
pseudoroegneria|gb167|FF342634
4569
726
85.2
globlastp


2905
LNU74
sorghum|09v1|SB06G022580
4570
726
85.2
globlastp


2906
LNU74
sorghum|gb161.crp|AI901612
4570
726
85.2
globlastp


2907
LNU74
sugarcane|10v1|CA073228
4568
726
85.2
globlastp


2908
LNU74
sugarcane|gb157.3|CA073228
4568
726
85.2
globlastp


2909
LNU74
oat|10v1|CA819453
4571
726
85.1
globlastp


2910
LNU74
brachypodium|09v1|DV479778
4572
726
85.1
globlastp


2911
LNU74
brachypodium|gb169|BE403473
4572
726
85.1
globlastp


2912
LNU74
rice|gb170|OS02G40880
4573
726
85.1
globlastp


2913
LNU74
sugarcane|gb157.3|CA084445
4574
726
85.1
globlastp


2914
LNU74
sugarcane|10v1|CA084445
4574
726
85.1
globlastp


2915
LNU74
wheat|gb164|BE429979
4575
726
84.4
globlastp


2916
LNU74
barley|gb157.3|BE421867
4576
726
84.3
globlastp


2917
LNU74
barley|gb157SOLEXA|BE421867
4576
726
84.3
globlastp


2918
LNU74
maize|gb170|LLDQ244985
4577
726
84.3
globlastp


2919
LNU74
sugarcane|gb157.3|CA119465
4578
726
84.3
globlastp


2920
LNU74
switchgrass|gb167|FE612757
4579
726
84.3
globlastp


2921
LNU74
switchgrass|gb167|FE644412
4579
726
84.3
globlastp


2922
LNU74
wheat|gb164|BE403473
4577
726
84.3
globlastp


2923
LNU74
wheat|gb164|TAU91834
4577
726
84.3
globlastp


2924
LNU74
oat|10v1|GO585574
4580
726
83.7
globlastp


2925
LNU74
fescue|gb161|DT690215
4581
726
83.7
globlastp


2926
LNU74
maize|gb170|LLDQ245227
4582
726
83.7
globlastp


2927
LNU74
wheat|gb164|BE400933
4582
726
83.7
globlastp


2928
LNU74
wheat|gb164|BE406339
4582
726
83.7
globlastp


2929
LNU74
eggplant|10v1|FS000049
4583
726
83.6
globlastp


2930
LNU74
millet|09v1|EVO454PM021136
4584
726
83.6
globlastp


2931
LNU74
sugarcane|10v1|CA073639
4585
726
83.6
globlastp


2932
LNU74
lovegrass|gb167|EH186458
4586
726
83.6
globlastp


2933
LNU74
maize|gb170|LLCF630355
4587
726
83.6
globlastp


2934
LNU74
maize|gb170|W21624
4587
726
83.6
globlastp


2935
LNU74
sorghum|09v1|SB02G031930
4588
726
83.6
globlastp


2936
LNU74
sorghum|gb161.crp|AW011692
4588
726
83.6
globlastp


2937
LNU74
sugarcane|gb157.3|CA073639
4589
726
83.6
globlastp


2938
LNU74
barley|gb157SOLEXA|AL500999
4590
726
83
globlastp


2939
LNU74
rye|gb164|BE587782
4591
726
82.84
glotblastn


2940
LNU74
eucalyptus|gb166|CT985211
4592
726
82.8
globlastp


2941
LNU74
maize|gb170|AI942046
4593
726
82.8
globlastp


2942
LNU74
maize|gb170|LLCF004305
4594
726
82.1
globlastp


2943
LNU74
brachypodium|gb169|BE400933
4595
726
81.4
globlastp


2944
LNU74
cotton|gb164|BM360100
4596
726
81.34
glotblastn


2945
LNU87
millet|09v1|EVO454PM017414
4597
731
91.5
globlastp


2946
LNU87
brachypodium|09v1|SRR031797S0046443
4598
731
84.46
glotblastn


2947
LNU87
brachypodium|gb169|BF145866
4599
731
82.4
glotblastn


2948
LNU87
rice|gb170|OS02G12900
4600
731
81.82
glotblastn


2949
LNU89
wheat|gb164|BE429720
4601
732
98.2
globlastp


2950
LNU89
barley|gb157SOLEXA|BE421794
4602
732
94.8
globlastp


2951
LNU89
oat|10v1|CN818133
4603
732
92.7
globlastp


2952
LNU89
brachypodium|09v1|DV473902
4604
732
91.1
globlastp


2953
LNU89
leymus|gb166|EG390106
4605
732
86.6
globlastp


2954
LNU89
switchgrass|gb167|FE650349
4606
732
80.9
globlastp


2955
LNU89
sorghum|09v1|SB01G028410
4607
732
80.1
globlastp


2956
LNU89
sorghum|gb161.crp|AA979933
4607
732
80.1
globlastp


2957
LNU98
sugarcane|10v1|CA151185
4608
734
92.84
glotblastn


2958
LNU98
sugarcane|gb157.3|CA151185
4609
734
91.69
glotblastn


2959
LNU128
radish|gb164|EV568872
4610
742
93.8
globlastp


2960
LNU128
canola|10v1|DY011559
4611
742
93.5
globlastp


2961
LNU128
canola|gb161|BQ704843
4612
742
93.2
globlastp


2962
LNU128
citrus|gb166|CX069720
4613
742
84.37
glotblastn


2963
LNU128
cotton|gb164|AJ513046
4614
742
84
globlastp


2964
LNU128
cacao|gb167|CU482048
4615
742
83.7
globlastp


2965
LNU128
grape|gb160|CB911162
4616
742
83.7
globlastp


2966
LNU128
potato|10v1|BG598087
4617
742
83.19
glotblastn


2967
LNU128
potato|gb157.2|BG598087
4618
742
83.19
glotblastn


2968
LNU128
solanum_phureja|09v1|SPHBG133047
4619
742
82.7
globlastp


2969
LNU128
tomato|09v1|BG133047
4620
742
82.7
globlastp


2970
LNU128
tomato|gb164|BG133047
4620
742
82.7
globlastp


2971
LNU128
eggplant|10v1|FS026981
4621
742
82.4
globlastp


2972
LNU128
cucumber|09v1|CSCRP021916
4622
742
82.3
globlastp


2973
LNU128
cassava|09v1|DV441652
4623
742
81.9
globlastp


2974
LNU128
kiwi|gb166|FG489702
4624
742
81.6
globlastp


2975
LNU128
rice|gb170|OS12G07720
4625
742
81.5
globlastp


2976
LNU128
castorbean|09v1|GE635249
4626
742
81
globlastp


2977
LNU128
castorbean|gb160|MDL29648M002000
4626
742
81
globlastp


2978
LNU128
sugarcane|10v1|CA074195
4627
742
80.9
globlastp


2979
LNU128
sugarcane|gb157.3|CA074195
4627
742
80.9
globlastp


2980
LNU128
switchgrass|gb167|FE607245
4628
742
80.9
globlastp


2981
LNU128
poplar|gb170|AI164310
4629
742
80.88
glotblastn


2982
LNU128
peanut|gb171|GO332421
4630
742
80.83
glotblastn


2983
LNU128
sorghum|09v1|SB08G004780
4631
742
80.6
globlastp


2984
LNU128
sorghum|gb161.crp|BM324980
4631
742
80.6
globlastp


2985
LNU128
sorghum|09v1|SB01G045530
4632
742
80.6
globlastp


2986
LNU128
sorghum|gb161.crp|CB334193
4632
742
80.6
globlastp


2987
LNU128
soybean|gb168|BF520452
4633
742
80.5
globlastp


2988
LNU128
apple|gb157.3|CN495076
4634
742
80.3
globlastp


2989
LNU128
apple|gb171|CN495076
4634
742
80.3
globlastp


2990
LNU128
aquilegia|10v1|DR941443
4635
742
80.24
glotblastn


2991
LNU128
monkeyflower|10v1|GR109554
4636
742
80.24
glotblastn


2992
LNU128
sunflower|gb162|DY947958
4637
742
80.24
glotblastn


2993
LNU128
poplar|10v1|AI164310
4638
742
80.2
globlastp


2994
LNU129
arabidopsis_lyrata|09v1|JGIAL004858
4639
743
89.6
globlastp


2995
LNU129
canola|10v1|CD827308
4640
743
81.2
globlastp


2996
LNU129
canola|gb161|CD827308
4640
743
81.2
globlastp


2997
LNU135
arabidopsis_lyrata|09v1|JGIAL023957
4641
747
96.6
globlastp


2998
LNU135
thellungiella|gb167|BM985897
4642
747
92.5
globlastp


2999
LNU135
b_oleracea|gb161|EH416218
4643
747
88.4
globlastp


3000
LNU135
canola|10v1|CD821934
4643
747
88.4
globlastp


3001
LNU135
b_rapa|gb162|CA991582
4644
747
88
globlastp


3002
LNU135
canola|10v1|CD820689
4645
747
88
globlastp


3003
LNU135
canola|gb161|CD820689
4645
747
88
globlastp


3004
LNU135
canola|10v1|CX189037
4646
747
87.3
globlastp


3005
LNU135
radish|gb164|EW735630
4647
747
86.9
globlastp


3006
LNU135
radish|gb164|EV567321
4648
747
86.5
globlastp


3007
LNU135
canola|gb161|CD821934
4649
747
84.6
globlastp


3008
LNU135
cleome_gynandra|10v1|SRR015532S0008526
4650
747
82.4
globlastp


3009
LNU140
arabidopsis_lyrata|09v1|JGIAL005684
4651
748
90
globlastp


3010
LNU140
cassava|09v1|DV446155
4652
748
81.8
globlastp


3011
LNU140
citrus|gb166|CB250310
4653
748
80.29
glotblastn


3012
LNU150
cotton|gb164|BE052334
4654
752
96.9
globlastp


3013
LNU150
cacao|gb167|CU477864
4655
752
88.9
globlastp


3014
LNU150
heritiera|10v1|SRR005795S0012955
4656
752
86.9
globlastp


3015
LNU150
tea|10v1|CV013763
4657
752
83.5
globlastp


3016
LNU150
poplar|10v1|AI162436
4658
752
81.4
globlastp


3017
LNU171
barley|gb157SOLEXA|BE412872
4659
757
96.6
globlastp


3018
LNU171
barley|gb157.3|BE412872
4660
757
95.9
globlastp


3019
LNU171
barley|gb157.3|BI777448
4661
757
91.8
globlastp


3020
LNU171
barley|gb157SOLEXA|BI777448
4661
757
91.8
globlastp


3021
LNU171
wheat|gb164|AL822126
4662
757
87.07
glotblastn


3022
LNU171
wheat|gb164|BE415359
4663
757
85.7
globlastp


3023
LNU171
wheat|gb164|BG607128
4664
757
83.9
globlastp


3024
LNU171
wheat|gb164|CA727731
4665
757
81
globlastp


3025
LNU172
wheat|gb164|CV774671
4666
758
84.5
globlastp


3026
LNU172
wheat|gb164|BQ807177
4667
758
81.8
globlastp


3027
LNU172
wheat|gb164|CA621288
4668
758
80.5
globlastp


3028
LNU179
arabidopsis_lyrata|09v1|JGIAL004871
4669
760
92.8
globlastp


3029
LNU212
arabidopsis_lyrata|09v1|JGIAL002964
4670
764
94.3
globlastp


3030
LNU212
radish|gb164|EV544090
4671
764
80.9
globlastp


3031
LNU212
canola|gb161|H74617
4672
764
80.8
globlastp


3032
LNU235
arabidopsis_lyrata|09v1|JGIAL003487
4673
770
93.92
glotblastn


3033
LNU235
radish|gb164|EV524630
4674
770
87.7
globlastp


3034
LNU235
thellungiella|gb167|BM985688
4675
770
87.42
glotblastn


3035
LNU235
b_rapa|gb162|L46407
4676
770
85.6
globlastp


3036
LNU250
arabidopsis_lyrata|09v1|JGIAL003223
4677
775
87
globlastp


3037
LNU253
lotus|gb157.2|BU494491
4678
777
84.39
glotblastn


3038
LNU253
lotus|09v1|LLBU494491
4679
777
83.4
globlastp


3039
LNU253
chickpea|09v2|DY475475
4680
777
80.39
glotblastn


3040
LNU256
arabidopsis_lyrata|09v1|JGIAL015756
4681
779
83.3
globlastp


3041
LNU260
arabidopsis_lyrata|09v1|JGIAL020384
4682
781
95.7
globlastp





Table 2: Provided are the homologous polypeptides and polynucleotides of the genes identified in Table 1 and of their cloned genes, which can increase nitrogen use efficiency, fertilizer use efficiency, yield, seed yield, growth rate, vigor, biomass, oil content, fiber yield, fiber quality, fiber length, abiotic stress tolerance and/or water use efficiency of a plant. Homology was calculated as % of identity over the aligned sequences. The query sequences were polypeptide sequences SEQ ID NOs: 468-706, and 707-784 and the subject sequences are polypeptide sequences or polynucleotide sequences which were dynamically translated in all six reading frames identified in the database based on greater than 80% identity to the query polypeptide sequences.


“Polyp.” = polypeptide;


“Polyn.”—Polynucleotide.


Algor. = Algorithm.


“globlastp”—global homology using blastp;


“glotblastn”—global homology using tblastn.


“Hom.”—homologous.






The output of the functional genomics approach described herein is a set of genes highly predicted to improve nitrogen use efficiency, fertilizer use efficiency, yield, seed yield, growth rate, vigor, biomass, oil content, fiber yield, fiber length, fiber quality, abiotic stress tolerance and/or water use efficiency of a plant by increasing their expression.
Although each gene is predicted to have its own impact, modifying the mode of expression of more than one gene or gene product (RNA, polypeptide) is expected to provide an additive or synergistic effect on the desired trait (e.g., nitrogen use efficiency, fertilizer use efficiency, yield, growth rate, vigor, biomass, oil content, abiotic stress tolerance and/or water use efficiency of a plant). Altering the expression of each gene described here alone or of a set of genes together increases the overall yield and/or other agronomic important traits, hence expects to increase agricultural productivity.
Example 3
Production of Arabidopsis Transcriptom and High Throughput Correlation Analysis Using 44K Arabidopsis Oligonucleotide Micro-Array
In order to produce a high throughput correlation analysis comparing between plant phenotype and gene expression level, the present inventors utilized a Arabidopsis oligonucleotide micro-array, produced by Agilent Technologies [Hypertext Transfer Protocol://World Wide Web (dot) chem (dot) agilent (dot) com/Scripts/PDS (dot) asp?1 Page=50879]. The array oligonucleotide represents about 44,000 Arabidopsis genes and transcripts. To define correlations between the levels of RNA expression with NUE, yield components or vigor related parameters various plant characteristics of 14 different Arabidopsis ecotypes were analyzed. Among them, ten ecotypes encompassing the observed variance were selected for RNA expression analysis. The correlation between the RNA levels and the characterized parameters was analyzed using Pearson correlation test [Hypertext Transfer Protocol://World Wide Web (dot) davidmlane (dot) com/hyperstat/A34739 (dot) html].
Experimental Procedures
Analyzed Arabidopsis tissues—Two tissues of plants [leaves and stems] growing at two different nitrogen fertilization levels (1.5 mM Nitrogen or 6 mM Nitrogen) were sampled and RNA was extracted as described above. Each micro-array expression information tissue type has received a Set ID as summarized Table 3 below.







TABLE 3







Arabidopsis transcriptom experimental sets










Expression Set
Set ID







Leaves at 1.5 mM Nitrogen fertilization
A



Leaves at 6 mM Nitrogen fertilization
B



Stems at 1.5 mM Nitrogen fertilization
C



Stem at 6 mM Nitrogen fertilization
D







Table 3.






Arabidopsis yield components and vigor related parameters under different nitrogen fertilization levels assessment—10 Arabidopsis accessions in 2 repetitive plots each containing 8 plants per plot were grown at greenhouse. The growing protocol used was as follows: surface sterilized seeds were sown in Eppendorf tubes containing 0.5× Murashige-Skoog basal salt medium and grown at 23° C. under 12-hour light and 12-hour dark daily cycles for 10 days. Then, seedlings of similar size were carefully transferred to pots filled with a mix of perlite and peat in a 1:1 ratio. Constant nitrogen limiting conditions were achieved by irrigating the plants with a solution containing 1.5 mM inorganic nitrogen in the form of KNO3, supplemented with 2 mM CaCl2, 1.25 mM KH2PO4, 1.50 mM MgSO4, 5 mM KCl, 0.01 mM H3BO3 and microelements, while normal irrigation conditions was achieved by applying a solution of 6 mM inorganic nitrogen also in the form of KNO3, supplemented with 2 mM CaCl2, 1.25 mM KH2PO4, 1.50 mM MgSO4, 0.01 mM H3BO3 and microelements. To follow plant growth, trays were photographed the day nitrogen limiting conditions were initiated and subsequently every 3 days for about 15 additional days. Rosette plant area was then determined from the digital pictures. Image) software was used for quantifying the plant size from the digital pictures [Hypertext Transfer Protocol://rsb (dot) info (dot) nih (dot) gov/ij/] utilizing proprietary scripts designed to analyze the size of rosette area from individual plants as a function of time. The image analysis system included a personal desktop computer (Intel P4 3.0 GHz processor) and a public domain program—ImageJ 1.37 (Java based image processing program, which was developed at the U.S. National Institutes of Health and freely available on the internet [Hypertext Transfer Protocol://rsbweb (dot) nih (dot) gov/]. Next, analyzed data was saved to text files and processed using the JMP statistical analysis software (SAS institute). Data parameters collected are summarized in Table 4, hereinbelow.







TABLE 4







Arabidopsis correlated parameters (vectors)











Correlation



Correlated parameter with
Id














N 1.5 mM; Rosette Area at day 8 [cm2]
1



N 1.5 mM; Rosette Area at day 10 [cm2]
2



N 1.5 mM; Plot Coverage at day 8 [%]
3



N 1.5 mM; Plot Coverage at day 10 [%]
4



N 1.5 mM; Leaf Number at day 10
5



N 1.5 mM; Leaf Blade Area at day 10 [cm2]
6



N 1.5 mM; RGR of Rosette Area at day 3
7



[cm2/day]



N 1.5 mM; t50 Flowering [day]
8



N 1.5 mM; Dry Weight [gr/plant]
9



N 1.5 mM; Seed Yield [gr/plant]
10



N 1.5 mM; Harvest Index
11



N 1.5 mM; 1000 Seeds weight [gr]
12



N 1.5 mM; seed yield/rosette area at day 10
13



[gr/cm2]



N 1.5 mM; seed yield/leaf blade [gr/cm2]
14



N 1.5 mM; % Seed yield reduction compared
15



to N 6 mM



N 1.5 mM; % Biomass reduction compared to
16



N 6 mM



N 1.5 mM; N level/DW [SPAD unit/gr]
17



N 1.5 mM; DW/N level [gr/SPAD unit]
18



N 1.5 mM; seed yield/N level [gr/SPAD unit]
19



N 6 mM; Rosette Area at day 8 [cm2]
20



N 6 mM; Rosette Area at day 10 [cm2]
21



N 6 mM; Plot Coverage at day 8 [%]
22



N 6 mM; Plot Coverage at day 10 [%]
23



N 6 mM; Leaf Number at day 10
24



N 6 mM; Leaf Blade Area at day 10
25



N 6 mM; RGR of Rosette Area at day 3 [cm2/gr]
26



N 6 mM; t50 Flowering [day]
27



N 6 mM; Dry Weight [gr/plant]
28



N 6 mM; Seed Yield [gr/plant]
29



N 6 mM; Harvest Index
30



N 6 mM; 1000 Seeds weight [gr]
31



N 6 mM; seed yield/rosette area day at
32



day 10 [gr/cm2]



N 6 mM; seed yield/leaf blade [gr/cm2]
33



N 6 mM; N level/FW
34



N 6 mM; DW/N level [gr/SPAD unit]
35



N 6 mM; N level/DW (SPAD unit/gr plant)
36



N 6 mM; Seed yield/N unit [gr/SPAD unit]
37







Table 4. “N” = Nitrogen at the noted concentrations; “gr.” = grams; “SPAD” = chlorophyll levels; “t50” = time where 50% of plants flowered; “gr/SPAD unit” = plant biomass expressed in grams per unit of nitrogen in plant measured by SPAD. “DW” = plant dry weight; “N level/DW” = plant Nitrogen level measured in SPAD unit per plant biomass [gr]; “DW/N level” = plant biomass per plant [gr]/SPAD unit;






Assessment of NUE, yield components and vigor-related parameters—Ten Arabidopsis ecotypes were grown in trays, each containing 8 plants per plot, in a greenhouse with controlled temperature conditions for about 12 weeks. Plants were irrigated with different nitrogen concentration as described above depending on the treatment applied. During this time, data was collected documented and analyzed. Most of chosen parameters were analyzed by digital imaging.
Digital imaging—Greenhouse assay
An image acquisition system, which consists of a digital reflex camera (Canon EOS 400D) attached with a 55 mm focal length lens (Canon EF-S series) placed in a custom made Aluminum mount, was used for capturing images of plants planted in containers within an environmental controlled greenhouse. The image capturing process is repeated every 2-3 days starting at day 9-12 till day 16-19 (respectively) from transplanting.
An image processing system was used, which consists of a personal desktop computer (Intel P4 3.0 GHz processor) and a public domain program—ImageJ 1.37, Java based image processing software, which was developed at the U.S. National Institutes of Health and is freely available on the internet at Hypertext Transfer Protocol://rsbweb (dot) nih (dot) gov/. Images were captured in resolution of 10 Mega Pixels (3888×2592 pixels) and stored in a low compression JPEG (Joint Photographic Experts Group standard) format. Next, image processing output data was saved to text files and analyzed using the JMP statistical analysis software (SAS institute).
Leaf analysis—Using the digital analysis leaves data was calculated, including leaf number, leaf blade area, Rosette diameter and area.
Relative growth area rate: The relative growth rate of the rosette and the leaves was calculated according to Formula II as described above.
Seed yield and 1000 seeds weight—At the end of the experiment all seeds from all plots were collected and weighed in order to measure seed yield per plant in terms of total seed weight per plant (gr). For the calculation of 1000 seed weight, an average weight of 0.02 grams was measured from each sample, the seeds were scattered on a glass tray and a picture was taken. Using the digital analysis, the number of seeds in each sample was calculated.
Dry weight and seed yield—At the end of the experiment, plant were harvested and left to dry at 30° C. in a drying chamber. The biomass was separated from the seeds, weighed and divided by the number of plants. Dry weight=total weight of the vegetative portion above ground (excluding roots) after drying at 30° C. in a drying chamber.
Harvest Index—The harvest index was calculated using Formula IV as described above.
T50 days to flowering—Each of the repeats was monitored for flowering date. Days of flowering was calculated from sowing date till 50% of the plots flowered.
Plant nitrogen level—The chlorophyll content of leaves is a good indicator of the nitrogen plant status since the degree of leaf greenness is highly correlated to this parameter. Chlorophyll content was determined using a Minolta SPAD 502 chlorophyll meter and measurement was performed at time of flowering. SPAD meter readings were done on young fully developed leaf. Three measurements per leaf were taken per plot. Based on this measurement, parameters such as the ratio between seed yield per nitrogen unit [seed yield/N level=seed yield per plant [gr]/SPAD unit], plant DW per nitrogen unit [DW/N level=plant biomass per plant [g]/SPAD unit], and nitrogen level per gram of biomass [N level/DW=SPAD unit/plant biomass per plant (gr)] were calculated.
Percent of seed yield reduction—measures the amount of seeds obtained in plants when grown under nitrogen-limiting conditions compared to seed yield produced at normal nitrogen levels expressed in %.
Experimental Results
10 different Arabidopsis accessions (ecotypes) were grown and characterized for 37 parameters as described above. The average for each of the measured parameters was calculated using the JMP software and values are summarized in Table 5 below. Subsequent correlation analysis between the various transcriptom sets (Table 3) and the measured parameters was conducted (Tables 6 and 7 below). Following are the results integrated to the database.







TABLE 5







Measured parameters in Arabidopsis accessions









Ecotype

















Treatment
Line-1
Line-2
Line-3
Line-4
Line-5
Line-6
Line-7
Line-8
Line-9
Line-10




















N 1.5 mM;
0.760
0.709
1.061
1.157
0.996
1.000
0.910
0.942
1.118
0.638


Rosette Area


at day 8


N 1.5 mM;
1.430
1.325
1.766
1.971
1.754
1.832
1.818
1.636
1.996
1.150


Rosette Area


at day 10


N 1.5 mM;
3.221
3.003
4.497
4.902
4.220
4.238
3.858
3.990
4.738
2.705


Plot


Coverage %


at day 8


N 1.5 mM;
6.058
5.614
7.484
8.351
7.432
7.764
7.702
6.933
8.458
4.871


Plot


Coverage %


at day 10


N 1.5 mM;
6.875
7.313
7.313
7.875
7.938
7.750
7.625
7.188
8.625
5.929


Leaf


Number at


day 10


N 1.5 mM;
0.335
0.266
0.374
0.387
0.373
0.370
0.386
0.350
0.379
0.307


Leaf Blade


Area at day


10


N 1.5 mM;
0.631
0.793
0.502
0.491
0.605
0.720
0.825
0.646
0.668
0.636


RGR of


Rosette Area


at day 3


N 1.5 mM;
15.967
20.968
14.836
24.708
23.566
23.698
18.059
19.488
23.568
21.888


t50


Flowering


[day]


N 1.5 mM;
0.164
0.124
0.082
0.113
0.184
0.124
0.134
0.106
0.148
0.171


Dry Weight


[gr/plant]


N 1.5 mM;
0.032
0.025
0.023
0.010
0.006
0.009
0.032
0.019
0.012
0.014


Seed Yield


[gr/plant]


N 1.5 mM;
0.192
0.203
0.295
0.085
0.031
0.071
0.241
0.179
0.081
0.079


Harvest


Index


N 1.5 mM;
0.016
0.016
0.018
0.014
0.018
0.022
0.015
0.014
0.022
0.019


1000 Seeds


weight[gr]


N 1.5 mM;
0.022
0.019
0.014
0.005
0.003
0.005
0.018
0.013
0.007
0.012


seed yield/


rosette area


day at day


10


N 1.5 mM;
0.095
0.095
0.063
0.026
0.015
0.024
0.084
0.059
0.034
0.044


seed


yield/leaf


blade


N 1.5 mM;
72.559
84.701
78.784
87.996
91.820
92.622
76.710
81.938
91.301
85.757


% Seed yield


reduction


compared to


6 mM


N 1.5 mM;
60.746
76.706
78.560
78.140
62.972
78.641
73.192
83.068
77.190
70.120


% Biomass


reduction


compared to


6 mM


N 1.5 mM;
45.590


42.108
28.151

53.111


67.000


Spad/FW


N 1.5 mM;
167.300


241.061
157.823

194.977


169.343


SPAD/DW


N 1.5 mM;
0.006


0.004
0.006

0.005


0.006


DW/SPAD


N 1.5 mM;
0.001


0.000
0.000

0.001


0.000


seed


yield/spad


N 6 mM;
0.759
0.857
1.477
1.278
1.224
1.095
1.236
1.094
1.410
0.891


Rosette Area


at day 8


N 6 mM;
1.406
1.570
2.673
2.418
2.207
2.142
2.474
1.965
2.721
1.642


Rosette Area


at day 10


N 6 mM;
3.216
3.631
6.259
5.413
5.187
4.641
5.236
4.634
5.974
3.774


Plot


Coverage %


at day 8


N 6 mM;
5.957
6.654
11.324
10.244
9.352
9.076
10.485
8.327
11.528
6.958


Plot


Coverage %


at day 10


N 6 mM;
6.250
7.313
8.063
8.750
8.063
8.750
8.375
7.125
9.438
6.313


Leaf


Number at


day 10


N 6 mM;
0.342
0.315
0.523
0.449
0.430
0.430
0.497
0.428
0.509
0.405


Leaf Blade


Area at day


10


N 6 mM;
0.689
1.024
0.614
0.601
0.477
0.651
0.676
0.584
0.613
0.515


RGR of


Rosette Area


at day 3


N 6 mM; t50
16.371
20.500
14.635
24.000
23.378
23.595
15.033
19.750
22.887
18.804


Flowering


[day]


N 6 mM;
0.419
0.531
0.382
0.518
0.496
0.579
0.501
0.628
0.649
0.573


Dry Weight


[gr/plant]


N 6 mM;
0.116
0.165
0.108
0.082
0.068
0.119
0.139
0.107
0.138
0.095


Seed Yield


[gr/plant]


N 6 mM;
0.280
0.309
0.284
0.158
0.136
0.206
0.276
0.171
0.212
0.166


Harvest


Index


N 6 mM;
0.015
0.017
0.018
0.012
0.016
0.016
0.015
0.014
0.017
0.016


1000 Seeds


weight[gr]


N 6 mM;
0.082
0.106
0.041
0.034
0.031
0.056
0.057
0.055
0.051
0.058


seed yield/


rosette area


day at day


10


N 6 mM;
0.339
0.526
0.207
0.183
0.158
0.277
0.281
0.252
0.271
0.235


seed


yield/leaf


blade


N 6 mM;
22.489


28.268
17.641

33.323


39.003


Spad/FW


N 6 mM;
0.019


0.018
0.028

0.015


0.015


DW/SPAD


(biomass/N


unit)


N 6 mM;
53.705


54.625
35.548

66.479


68.054


spad/DW


(gN/g plant)


N 6 mM;
0.004


0.003
0.002

0.005


0.003


Seed yield/N


unit





Table 5.


Provided are the measured parameters under various treatments in various ecotypes (Arabidopsis accessions).













TABLE 6







Correlation between the expression level of selected LNU genes of some embodiments


of the invention in various tissues and the phenotypic performance under normal or


low nitrogen fertilization conditions across Arabidopsis accessions




















Correl.







Gene

P
Exp.
Set
Gene


Exp.
Correl.


Name
R
value
set
ID
Name
R
P value
set
Set ID



















LNU1
0.78
7.86E−03
A
6
LNU182
0.73
1.70E−02
A
8


LNU1
0.76
1.80E−02
C
5
LNU182
0.75
1.20E−02
A
8


LNU1
0.75
1.33E−02
A
2
LNU182
0.75
2.09E−02
C
8


LNU1
0.80
4.98E−03
A
1
LNU182
0.76
1.04E−02
C
8


LNU1
0.74
1.35E−02
A
1
LNU182
0.84
2.12E−03
B
24


LNU1
0.72
2.87E−02
C
1
LNU182
0.81
4.08E−03
B
24


LNU1
0.83
3.20E−03
B
25
LNU182
0.91
6.63E−04
D
24


LNU1
0.83
2.96E−03
B
21
LNU182
0.78
8.39E−03
D
24


LNU1
0.86
1.27E−03
B
20
LNU182
0.75
1.17E−02
B
27


LNU1
0.97
4.77E−03
B
35
LNU182
0.73
1.66E−02
B
27


LNU1
0.91
3.24E−02
B
35
LNU182
0.79
6.67E−03
D
27


LNU123
0.90
3.72E−02
A
18
LNU183
0.80
5.90E−03
C
10


LNU123
0.89
4.05E−02
B
35
LNU183
0.80
5.89E−03
C
14


LNU124
0.87
9.30E−04
A
11
LNU183
0.79
6.13E−03
C
13


LNU124
0.81
7.91E−03
C
11
LNU183
0.71
2.06E−02
B
31


LNU124
0.91
2.70E−04
C
11
LNU183
0.80
5.94E−03
D
30


LNU124
0.72
1.95E−02
A
10
LNU183
0.72
2.94E−02
D
32


LNU124
0.91
2.78E−04
A
10
LNU184
0.87
1.08E−03
C
11


LNU124
0.83
6.08E−03
C
10
LNU184
0.89
4.09E−02
C
19


LNU124
0.89
6.50E−04
C
10
LNU184
0.88
4.83E−02
B
37


LNU124
0.96
9.63E−03
A
19
LNU184
0.93
1.98E−02
B
37


LNU124
0.98
2.55E−03
C
19
LNU184
0.93
2.08E−02
D
37


LNU124
0.84
2.31E−03
A
14
LNU184
0.89
4.04E−02
B
36


LNU124
0.75
1.96E−02
C
14
LNU185
0.99
1.83E−03
C
18


LNU124
0.89
5.45E−04
C
14
LNU186
0.72
1.88E−02
C
15


LNU124
0.86
1.25E−03
A
13
LNU186
0.84
2.46E−03
C
8


LNU124
0.79
1.20E−02
C
13
LNU186
0.73
1.71E−02
D
27


LNU124
0.85
2.04E−03
C
13
LNU187
0.82
6.47E−03
D
26


LNU124
0.81
4.19E−03
B
30
LNU187
0.74
2.18E−02
D
33


LNU124
0.89
6.61E−04
D
30
LNU187
0.88
4.65E−02
B
37


LNU124
0.92
2.79E−02
B
37
LNU206
0.72
1.82E−02
C
16


LNU124
0.94
1.63E−02
D
37
LNU206
0.71
2.02E−02
C
16


LNU125
0.76
1.06E−02
A
11
LNU206
0.71
2.05E−02
A
2


LNU125
0.72
1.80E−02
A
11
LNU206
0.84
2.24E−03
A
1


LNU125
0.81
4.61E−03
A
10
LNU206
0.82
3.92E−03
A
1


LNU125
0.83
3.17E−03
A
10
LNU206
0.75
1.24E−02
B
20


LNU125
0.84
4.66E−03
C
10
LNU206
0.72
1.90E−02
B
20


LNU125
0.95
1.30E−02
A
19
LNU207
0.79
6.36E−03
A
11


LNU125
0.88
4.88E−02
C
19
LNU210
0.73
2.64E−02
C
11


LNU125
0.82
3.78E−03
A
14
LNU210
0.74
1.36E−02
A
10


LNU125
0.78
7.43E−03
A
14
LNU210
0.70
2.33E−02
A
10


LNU125
0.83
5.88E−03
C
14
LNU210
0.74
1.38E−02
A
14


LNU125
0.72
1.84E−02
A
13
LNU210
0.73
1.67E−02
A
14


LNU125
0.72
1.96E−02
A
13
LNU210
0.72
1.87E−02
A
14


LNU125
0.82
6.30E−03
C
13
LNU210
0.78
8.31E−03
A
13


LNU125
0.82
3.37E−03
B
30
LNU210
0.74
1.45E−02
A
13


LNU125
0.92
1.78E−04
B
30
LNU210
0.73
1.62E−02
A
13


LNU125
0.71
2.11E−02
D
30
LNU210
0.74
1.49E−02
B
30


LNU125
0.79
5.99E−03
B
26
LNU210
0.71
2.18E−02
B
30


LNU125
0.78
7.61E−03
B
29
LNU211
0.82
3.68E−03
C
15


LNU125
0.78
8.34E−03
B
29
LNU211
0.77
9.05E−03
C
15


LNU125
0.91
3.07E−02
B
37
LNU211
0.89
5.65E−04
C
8


LNU125
0.91
3.16E−02
B
37
LNU211
0.84
2.25E−03
C
8


LNU126
0.77
9.60E−03
A
11
LNU211
0.80
4.99E−03
D
27


LNU126
0.76
1.13E−02
C
11
LNU211
0.77
8.96E−03
D
27


LNU126
0.87
1.01E−03
A
10
LNU213
0.75
1.26E−02
C
9


LNU126
0.74
2.28E−02
C
10
LNU213
0.76
1.07E−02
A
8


LNU126
0.93
1.08E−04
C
10
LNU213
0.75
1.17E−02
B
27


LNU126
0.92
2.83E−02
C
19
LNU215
0.80
5.41E−03
A
11


LNU126
0.83
3.21E−03
A
14
LNU215
0.77
9.84E−03
A
11


LNU126
0.72
3.00E−02
C
14
LNU215
0.85
1.72E−03
C
11


LNU126
0.94
4.77E−05
C
14
LNU215
0.75
1.16E−02
C
11


LNU126
0.87
1.05E−03
A
13
LNU215
0.76
1.09E−02
A
7


LNU126
0.78
1.31E−02
C
13
LNU215
0.74
1.49E−02
A
7


LNU126
0.95
3.71E−05
C
13
LNU215
0.90
3.85E−04
A
10


LNU126
0.94
1.81E−02
A
17
LNU215
0.89
6.05E−04
A
10


LNU126
0.74
1.35E−02
B
30
LNU215
0.77
8.62E−03
C
10


LNU126
0.88
9.14E−04
D
30
LNU215
0.91
3.00E−02
A
19


LNU126
0.72
1.94E−02
D
32
LNU215
0.91
3.33E−02
A
19


LNU126
0.91
3.13E−02
B
36
LNU215
0.95
1.52E−02
C
19


LNU127
0.73
1.55E−02
A
12
LNU215
0.90
4.04E−04
A
14


LNU127
0.81
4.57E−03
A
11
LNU215
0.87
9.88E−04
A
14


LNU127
0.87
2.17E−03
C
11
LNU215
0.86
1.39E−03
A
13


LNU127
0.81
8.03E−03
C
11
LNU215
0.84
2.45E−03
A
13


LNU127
0.74
1.49E−02
C
11
LNU215
0.90
4.13E−04
B
30


LNU127
0.85
3.93E−03
C
10
LNU215
0.82
3.99E−03
B
30


LNU127
0.75
1.95E−02
C
10
LNU215
0.71
2.18E−02
B
26


LNU127
0.79
6.97E−03
C
10
LNU218
0.82
7.15E−03
C
11


LNU127
0.90
3.54E−02
A
19
LNU218
0.74
2.21E−02
C
11


LNU127
0.92
2.63E−02
C
19
LNU218
0.70
2.31E−02
C
11


LNU127
0.79
1.13E−02
C
14
LNU218
0.73
1.75E−02
A
14


LNU127
0.77
9.09E−03
C
14
LNU218
0.74
1.42E−02
A
14


LNU127
0.80
9.01E−03
C
13
LNU218
0.72
2.72E−02
C
14


LNU127
0.78
7.52E−03
C
13
LNU218
0.74
1.41E−02
C
14


LNU127
0.73
1.73E−02
C
13
LNU218
0.75
1.19E−02
A
13


LNU127
0.89
4.17E−02
A
17
LNU218
0.76
1.05E−02
A
13


LNU127
0.76
1.08E−02
B
30
LNU218
0.72
3.01E−02
C
13


LNU127
0.83
5.36E−03
D
30
LNU218
0.78
7.56E−03
C
13


LNU127
0.81
7.93E−03
D
30
LNU219
0.79
6.18E−03
C
16


LNU127
0.90
3.61E−02
B
37
LNU219
0.74
1.41E−02
C
16


LNU127
0.89
4.37E−02
B
36
LNU219
0.78
7.39E−03
A
2


LNU127
0.92
2.87E−02
B
34
LNU219
0.76
1.13E−02
A
2


LNU128
0.77
9.80E−03
A
11
LNU219
0.81
4.95E−03
A
1


LNU128
0.76
1.13E−02
A
11
LNU219
0.79
6.02E−03
A
1


LNU128
0.81
4.54E−03
C
11
LNU219
0.97
4.90E−03
A
17


LNU128
0.72
1.85E−02
A
10
LNU219
0.96
8.96E−03
A
17


LNU128
0.97
7.59E−03
A
19
LNU219
0.74
2.26E−02
D
25


LNU128
0.95
1.40E−02
A
19
LNU219
0.74
2.40E−02
D
25


LNU128
0.96
1.12E−02
C
19
LNU219
0.74
1.35E−02
B
24


LNU128
0.79
6.18E−03
B
30
LNU219
0.71
2.14E−02
B
24


LNU128
0.73
1.73E−02
B
30
LNU219
0.78
1.40E−02
D
24


LNU128
0.80
9.72E−03
D
30
LNU219
0.77
1.45E−02
D
24


LNU128
0.79
1.22E−02
D
29
LNU219
0.74
1.53E−02
D
24


LNU128
0.91
3.39E−02
B
37
LNU219
0.73
1.76E−02
D
24


LNU128
0.96
1.03E−02
B
37
LNU219
0.78
7.47E−03
B
21


LNU129
0.74
1.46E−02
A
16
LNU219
0.72
1.97E−02
B
21


LNU129
0.70
2.35E−02
A
16
LNU219
0.78
1.36E−02
D
21


LNU129
0.82
6.97E−03
C
11
LNU219
0.77
1.46E−02
D
21


LNU129
0.81
4.89E−03
C
11
LNU219
0.72
1.83E−02
B
20


LNU129
0.79
5.99E−03
C
11
LNU219
0.70
2.31E−02
B
20


LNU129
0.79
1.12E−02
C
10
LNU219
0.75
1.30E−02
B
20


LNU129
0.73
2.65E−02
C
14
LNU225
0.76
1.15E−02
A
12


LNU129
0.73
2.56E−02
C
13
LNU225
0.71
2.08E−02
A
5


LNU129
0.95
1.29E−02
A
17
LNU225
0.71
2.22E−02
A
5


LNU129
0.94
1.61E−02
A
17
LNU225
0.79
6.89E−03
A
2


LNU129
0.92
2.87E−02
A
17
LNU225
0.82
4.02E−03
A
2


LNU129
0.73
2.48E−02
D
30
LNU225
0.80
5.59E−03
A
2


LNU130
0.87
1.05E−03
A
11
LNU225
0.78
8.33E−03
A
2


LNU130
0.92
1.88E−04
A
10
LNU225
0.80
1.03E−02
C
2


LNU130
0.96
1.13E−02
A
19
LNU225
0.77
1.50E−02
C
2


LNU130
0.89
4.83E−04
A
14
LNU225
0.76
1.06E−02
C
2


LNU130
0.89
5.74E−04
A
13
LNU225
0.74
1.49E−02
C
2


LNU130
0.85
1.96E−03
B
30
LNU225
0.73
1.76E−02
C
2


LNU130
0.78
8.11E−03
D
26
LNU225
0.75
1.22E−02
A
1


LNU130
0.73
1.70E−02
D
33
LNU225
0.75
1.30E−02
A
1


LNU130
0.97
6.99E−03
B
37
LNU225
0.74
2.23E−02
C
1


LNU131
0.77
8.68E−03
A
6
LNU225
0.73
1.66E−02
C
1


LNU131
0.75
1.26E−02
A
6
LNU225
0.99
7.77E−04
A
17


LNU131
0.81
4.74E−03
A
2
LNU225
0.95
1.23E−02
A
17


LNU131
0.80
5.22E−03
A
2
LNU225
0.98
2.52E−03
A
17


LNU131
0.86
1.29E−03
A
1
LNU225
0.98
2.54E−03
A
17


LNU131
0.82
3.59E−03
A
1
LNU225
0.99
5.55E−04
C
17


LNU131
0.92
2.64E−02
A
17
LNU225
0.99
1.27E−03
C
17


LNU131
0.88
4.64E−02
A
17
LNU225
0.89
4.56E−02
C
17


LNU131
0.81
4.18E−03
B
25
LNU225
0.82
3.45E−03
B
24


LNU131
0.74
1.40E−02
B
25
LNU225
0.80
5.23E−03
B
24


LNU131
0.82
3.65E−03
B
24
LNU225
0.82
3.67E−03
B
24


LNU131
0.70
2.33E−02
B
24
LNU225
0.75
1.23E−02
B
24


LNU131
0.84
2.46E−03
B
21
LNU225
0.75
1.26E−02
B
24


LNU131
0.75
1.16E−02
B
21
LNU225
0.78
1.24E−02
D
24


LNU131
0.79
6.07E−03
B
20
LNU225
0.83
3.21E−03
D
24


LNU131
0.74
1.36E−02
B
20
LNU225
0.81
4.76E−03
D
24


LNU131
0.95
1.41E−02
B
35
LNU225
0.77
8.51E−03
D
24


LNU131
0.91
3.29E−02
B
35
LNU234
0.92
1.64E−04
A
11


LNU132
0.94
3.82E−05
A
11
LNU234
0.76
1.03E−02
A
11


LNU132
0.72
2.85E−02
C
11
LNU234
0.83
2.68E−03
C
11


LNU132
0.78
8.12E−03
C
11
LNU234
0.83
3.05E−03
C
11


LNU132
0.72
1.81E−02
A
10
LNU234
0.80
5.47E−03
A
10


LNU132
0.84
2.47E−03
A
10
LNU234
0.85
3.92E−03
C
10


LNU132
0.79
6.66E−03
C
10
LNU234
0.97
5.68E−03
A
19


LNU132
0.94
1.85E−02
A
19
LNU234
0.97
6.49E−03
C
19


LNU132
0.76
1.00E−02
A
14
LNU234
0.96
1.08E−02
C
19


LNU132
0.72
1.84E−02
C
14
LNU234
0.81
8.21E−03
C
14


LNU132
0.74
1.49E−02
A
13
LNU234
0.81
8.76E−03
C
13


LNU132
0.74
1.44E−02
C
13
LNU234
0.94
1.95E−02
A
17


LNU132
0.72
1.89E−02
B
30
LNU234
0.89
4.23E−02
A
17


LNU132
0.89
4.59E−02
B
37
LNU234
0.90
3.87E−02
C
17


LNU132
0.97
7.13E−03
B
37
LNU234
0.90
8.60E−04
D
30


LNU133
0.76
1.12E−02
A
7
LNU234
0.79
1.05E−02
D
30


LNU133
0.71
2.03E−02
A
7
LNU234
0.87
1.12E−03
D
30


LNU133
0.81
7.58E−03
C
7
LNU234
0.82
4.00E−03
D
30


LNU133
0.81
4.47E−03
C
7
LNU234
0.83
5.75E−03
D
29


LNU133
0.82
3.45E−03
B
29
LNU234
0.76
1.69E−02
D
29


LNU133
0.77
9.67E−03
B
29
LNU234
0.75
2.09E−02
D
29


LNU133
0.85
3.64E−03
D
29
LNU234
0.74
2.30E−02
D
32


LNU133
0.79
7.02E−03
D
29
LNU234
0.72
2.75E−02
D
33


LNU133
0.89
4.18E−02
D
37
LNU234
0.97
6.94E−03
B
37


LNU134
0.70
2.42E−02
A
12
LNU234
0.95
1.25E−02
D
37


LNU134
0.74
1.39E−02
A
7
LNU234
0.89
4.04E−02
D
37


LNU134
0.73
1.69E−02
A
7
LNU234
0.91
3.01E−02
D
36


LNU134
0.75
2.08E−02
C
7
LNU235
0.74
1.44E−02
A
11


LNU134
0.72
1.85E−02
C
2
LNU235
0.73
1.66E−02
A
11


LNU134
0.98
2.66E−03
A
17
LNU235
0.76
1.06E−02
C
11


LNU134
0.73
2.51E−02
D
30
LNU235
0.73
1.70E−02
A
10


LNU134
0.80
5.04E−03
B
24
LNU235
0.73
1.75E−02
A
14


LNU134
0.83
3.21E−03
B
24
LNU235
0.73
2.43E−02
C
14


LNU134
0.76
1.85E−02
D
24
LNU235
0.78
8.39E−03
A
13


LNU134
0.83
2.67E−03
D
24
LNU235
0.79
1.20E−02
D
30


LNU134
0.77
9.31E−03
B
26
LNU235
0.86
2.99E−03
D
26


LNU134
0.79
6.09E−03
B
26
LNU235
0.83
5.85E−03
D
32


LNU134
0.86
3.03E−03
D
26
LNU235
0.84
4.39E−03
D
33


LNU134
0.80
5.60E−03
D
26
LNU24
0.71
2.18E−02
A
11


LNU134
0.82
3.60E−03
B
29
LNU24
0.85
1.97E−03
A
11


LNU134
0.79
6.62E−03
B
29
LNU24
0.78
7.95E−03
C
11


LNU134
0.87
2.40E−03
D
29
LNU24
0.71
2.01E−02
A
10


LNU134
0.72
1.96E−02
D
29
LNU24
0.73
1.60E−02
A
10


LNU134
0.71
2.04E−02
B
32
LNU24
0.79
1.15E−02
C
10


LNU134
0.86
1.49E−03
B
32
LNU24
0.93
2.40E−02
A
19


LNU134
0.85
3.42E−03
D
32
LNU24
0.77
9.72E−03
A
8


LNU134
0.86
1.25E−03
D
32
LNU24
0.73
1.73E−02
B
27


LNU134
0.82
3.64E−03
B
33
LNU242
0.82
3.31E−03
A
7


LNU134
0.89
5.09E−04
B
33
LNU242
0.71
2.09E−02
A
7


LNU134
0.91
7.35E−04
D
33
LNU242
0.88
1.66E−03
C
7


LNU134
0.89
5.29E−04
D
33
LNU242
0.71
2.27E−02
C
7


LNU135
0.85
1.71E−03
A
11
LNU242
0.70
3.46E−02
C
10


LNU135
0.72
1.90E−02
A
10
LNU242
0.91
3.06E−02
C
17


LNU135
0.93
2.21E−02
C
17
LNU242
0.85
1.63E−03
B
29


LNU136
0.79
6.32E−03
A
11
LNU242
0.81
4.09E−03
B
29


LNU136
0.96
7.92E−03
A
17
LNU242
0.83
6.09E−03
D
29


LNU136
0.95
1.21E−02
A
17
LNU242
0.87
9.49E−04
D
29


LNU136
0.90
3.77E−02
C
17
LNU247
0.78
8.28E−03
A
12


LNU136
0.78
1.41E−02
D
30
LNU247
0.85
1.84E−03
A
12


LNU136
0.88
8.99E−04
B
26
LNU247
0.75
1.94E−02
C
12


LNU136
0.80
5.26E−03
B
33
LNU247
0.80
4.99E−03
C
12


LNU136
0.93
2.21E−02
D
37
LNU249
0.70
2.28E−02
A
11


LNU14
0.92
2.50E−02
C
17
LNU249
0.85
3.90E−03
C
7


LNU14
0.72
1.89E−02
B
25
LNU249
0.87
1.07E−03
A
10


LNU14
0.91
3.18E−02
D
36
LNU249
0.95
1.35E−02
A
19


LNU140
0.93
2.06E−02
C
18
LNU249
0.95
1.78E−05
A
14


LNU140
0.81
7.85E−03
C
7
LNU249
0.92
1.31E−04
A
13


LNU140
0.97
7.30E−03
A
19
LNU249
0.83
2.68E−03
B
30


LNU140
0.77
9.73E−03
A
8
LNU249
0.82
6.70E−03
D
30


LNU140
0.85
1.72E−03
B
26
LNU249
0.75
1.88E−02
D
30


LNU140
0.74
1.42E−02
B
29
LNU249
0.71
2.07E−02
B
26


LNU140
0.78
1.24E−02
D
29
LNU249
0.81
4.45E−03
B
26


LNU140
0.81
4.46E−03
B
32
LNU249
0.80
9.94E−03
D
26


LNU140
0.75
2.12E−02
D
32
LNU249
0.80
5.30E−03
B
29


LNU140
0.89
5.19E−04
B
33
LNU249
0.74
1.42E−02
B
29


LNU140
0.76
1.65E−02
D
33
LNU249
0.82
7.33E−03
D
29


LNU140
0.96
1.09E−02
B
37
LNU249
0.81
8.47E−03
D
29


LNU140
0.75
1.26E−02
B
27
LNU249
0.80
5.30E−03
B
32


LNU15
0.70
2.30E−02
A
9
LNU249
0.85
1.90E−03
B
32


LNU170
0.74
1.40E−02
A
12
LNU249
0.74
2.31E−02
D
32


LNU170
0.76
1.66E−02
C
12
LNU249
0.82
3.37E−03
B
33


LNU170
0.73
1.71E−02
A
16
LNU249
0.83
3.29E−03
B
33


LNU170
0.73
1.59E−02
C
16
LNU249
0.79
1.18E−02
D
33


LNU170
0.82
4.00E−03
A
2
LNU249
0.90
3.49E−02
B
37


LNU170
0.79
6.10E−03
A
2
LNU250
0.78
7.39E−03
A
11


LNU170
0.80
9.44E−03
C
2
LNU250
0.71
2.17E−02
A
7


LNU170
0.79
6.07E−03
C
2
LNU250
0.79
6.58E−03
A
10


LNU170
0.88
8.83E−04
A
1
LNU250
0.95
3.35E−05
A
10


LNU170
0.88
6.87E−04
A
1
LNU250
0.91
3.18E−02
A
19


LNU170
0.84
4.72E−03
C
1
LNU250
0.97
6.08E−03
A
19


LNU170
0.87
9.86E−04
C
1
LNU250
0.83
2.77E−03
A
14


LNU170
0.81
8.77E−03
D
25
LNU250
0.93
7.59E−05
A
14


LNU170
0.74
1.45E−02
D
25
LNU250
0.83
2.76E−03
A
13


LNU170
0.81
4.61E−03
B
24
LNU250
0.92
1.63E−04
A
13


LNU170
0.78
8.26E−03
B
24
LNU250
0.70
3.50E−02
C
13


LNU170
0.73
1.72E−02
B
24
LNU250
0.80
5.53E−03
B
30


LNU170
0.80
9.62E−03
D
24
LNU250
0.78
7.75E−03
B
29


LNU170
0.78
7.72E−03
D
24
LNU250
0.71
2.09E−02
B
29


LNU170
0.80
5.86E−03
B
21
LNU250
0.82
3.82E−03
B
32


LNU170
0.81
4.63E−03
B
21
LNU250
0.75
1.17E−02
B
33


LNU170
0.90
9.72E−04
D
21
LNU250
0.91
3.17E−02
B
37


LNU170
0.86
1.38E−03
D
21
LNU250
0.95
1.24E−02
B
37


LNU170
0.80
5.15E−03
B
20
LNU251
0.75
1.32E−02
A
6


LNU170
0.84
2.62E−03
B
20
LNU251
0.79
6.02E−03
A
2


LNU170
0.90
1.04E−03
D
20
LNU251
0.72
1.96E−02
A
1


LNU170
0.89
6.02E−04
D
20
LNU251
0.73
1.68E−02
B
24


LNU175
0.80
5.82E−03
A
11
LNU251
0.92
2.57E−02
B
35


LNU175
0.90
3.34E−04
C
11
LNU254
0.84
2.37E−03
C
15


LNU175
0.98
3.47E−03
C
19
LNU254
0.88
8.39E−04
C
8


LNU175
0.93
2.38E−02
D
37
LNU254
0.82
4.01E−03
D
27


LNU175
0.88
4.60E−02
B
36
LNU255
0.78
8.00E−03
A
10


LNU175
0.91
2.97E−02
B
34
LNU255
0.89
4.54E−02
A
19


LNU177
0.75
1.21E−02
A
11
LNU255
0.91
3.16E−02
A
17


LNU177
0.73
1.66E−02
C
11
LNU255
0.96
8.73E−03
B
37


LNU177
0.73
1.68E−02
C
10
LNU255
0.99
5.16E−04
B
36


LNU177
0.79
6.51E−03
B
31
LNU255
0.92
2.49E−02
B
34


LNU177
0.86
1.60E−03
D
30
LNU255
0.91
3.34E−02
B
34


LNU177
0.77
9.90E−03
D
32
LNU256
0.76
1.78E−02
C
11


LNU179
0.80
5.62E−03
A
11
LNU256
0.78
7.59E−03
A
5


LNU179
0.87
9.87E−04
A
11
LNU256
0.78
1.32E−02
D
31


LNU179
0.90
4.59E−04
C
11
LNU256
0.71
2.19E−02
D
30


LNU179
0.76
1.15E−02
A
10
LNU256
0.71
2.05E−02
B
24


LNU179
0.92
1.92E−04
A
10
LNU257
0.85
1.75E−03
C
11


LNU179
0.72
1.95E−02
C
10
LNU257
0.74
1.43E−02
A
7


LNU179
0.96
1.11E−02
A
19
LNU257
0.73
1.70E−02
A
7


LNU179
0.91
3.44E−02
C
19
LNU257
0.82
4.07E−03
C
10


LNU179
0.84
2.27E−03
A
14
LNU257
0.90
3.65E−02
C
19


LNU179
0.83
2.82E−03
A
13
LNU257
0.82
3.37E−03
C
14


LNU179
0.97
6.67E−03
B
37
LNU257
0.83
2.91E−03
C
13


LNU180
0.79
6.02E−03
A
7
LNU257
0.81
4.78E−03
D
30


LNU180
0.76
1.04E−02
A
7
LNU258
0.75
1.31E−02
A
11


LNU180
0.72
1.92E−02
A
10
LNU258
0.79
6.36E−03
C
11


LNU180
0.77
1.46E−02
C
10
LNU258
0.76
1.01E−02
A
10


LNU180
0.91
3.24E−02
A
19
LNU258
0.80
5.41E−03
C
10


LNU180
0.93
1.99E−02
A
19
LNU258
0.92
2.45E−02
C
19


LNU180
0.92
2.57E−02
A
19
LNU258
0.79
6.92E−03
A
14


LNU180
0.70
2.41E−02
A
14
LNU258
0.87
1.11E−03
C
14


LNU180
0.74
2.29E−02
C
13
LNU258
0.83
2.76E−03
A
13


LNU180
0.92
2.74E−02
A
17
LNU258
0.86
1.56E−03
C
13


LNU180
0.86
1.55E−03
B
29
LNU258
0.76
1.09E−02
D
30


LNU180
0.81
4.45E−03
B
29
LNU260
0.86
1.39E−03
C
11


LNU180
0.99
2.08E−03
B
37
LNU260
0.91
2.73E−04
C
10


LNU180
0.88
4.67E−02
B
37
LNU260
0.93
2.34E−02
C
19


LNU180
0.98
4.69E−03
B
36
LNU260
0.88
7.70E−04
C
14


LNU180
0.89
4.53E−02
B
34
LNU260
0.84
2.42E−03
C
13


LNU181
0.72
1.80E−02
A
2
LNU260
0.78
8.14E−03
D
30


LNU181
0.80
5.37E−03
A
1
LNU260
0.97
7.52E−03
D
37


LNU181
0.74
1.46E−02
A
15
LNU261
0.75
1.20E−02
A
12


LNU181
0.82
4.04E−03
B
24
LNU261
0.75
1.32E−02
A
12


LNU181
0.75
1.29E−02
B
21
LNU261
0.83
2.88E−03
A
6


LNU181
0.76
1.05E−02
B
20
LNU261
0.78
7.38E−03
A
1


LNU181
0.91
3.17E−02
B
35
LNU261
0.73
2.66E−02
D
31


LNU182
0.71
3.32E−02
C
11
LNU261
0.71
3.36E−02
D
31


LNU182
0.71
2.09E−02
A
6
LNU261
0.71
2.24E−02
B
25


LNU182
0.79
6.26E−03
A
5
LNU261
0.71
2.04E−02
B
24


LNU182
0.71
2.28E−02
A
5
LNU261
0.80
5.03E−03
B
21


LNU182
0.97
8.06E−06
C
5
LNU261
0.84
2.50E−03
B
20


LNU182
0.78
7.68E−03
C
5
LNU261
0.94
1.78E−02
B
35


LNU182
0.78
7.95E−03
A
2
LNU262
0.83
3.15E−03
A
12


LNU182
0.79
6.72E−03
A
2
LNU262
0.78
1.34E−02
C
2


LNU182
0.80
1.04E−02
C
2
LNU262
0.79
6.53E−03
A
15


LNU182
0.72
1.96E−02
C
2
LNU262
0.80
5.50E−03
A
15


LNU182
0.76
1.14E−02
A
1
LNU262
0.73
1.62E−02
C
15


LNU182
0.77
8.53E−03
A
1
LNU262
0.72
1.97E−02
A
8


LNU182
0.75
2.05E−02
C
1
LNU262
0.88
8.15E−04
A
8


LNU182
0.71
2.03E−02
C
1
LNU262
0.85
1.74E−03
C
8


LNU182
0.77
8.65E−03
A
15
LNU262
0.81
7.58E−03
D
24


LNU182
0.75
1.33E−02
A
15
LNU262
0.70
2.37E−02
B
27


LNU182
0.82
6.29E−03
C
15
LNU262
0.85
2.02E−03
B
27


LNU182
0.82
3.37E−03
C
15
LNU8
0.75
2.09E−02
C
5





Table 6.


“Correl. Set ID”—correlation set ID according to the correlated parameters Table above.













TABLE 7







Correlation between the expression level of selected LNU orthologs genes of some


embodiments of the invention in various tissues and the phenotypic performance


under normal or low nitrogen fertilization conditions across Arabidopsis accessions


















P
Exp.
Correl.


P
Exp.
Correl.


Gene Name
R
value
Set
Set ID
Gene Name
R
value
Set
Set ID



















LNU219_H1
0.88
6.94E−04
B
12
LNU45_H11
0.91
3.06E−02
A
17


LNU76_H3
0.77
9.83E−03
B
12
LNU46_H3
0.89
4.47E−02
A
17


LNU219_H1
0.71
2.17E−02
B
15
LNU46_H3
0.91
3.04E−02
B
36


LNU256_H0
0.96
1.14E−02
B
17
LNU219_H1
0.76
1.66E−02
D
27


LNU219_H1
0.79
7.10E−03
C
15
LNU219_H1
0.78
7.31E−03
C
27


LNU76_H3
0.74
1.45E−02
B
31
LNU7_H5
0.96
9.76E−03
B
35


LNU76_H3
0.70
2.40E−02
B
28
LNU219_H1
0.76
1.77E−02
D
5


LNU46_H5
0.73
1.67E−02
B
28
LNU7_H5
0.70
3.41E−02
D
5


LNU7_H5
0.70
2.32E−02
A
25
LNU7_H4
0.79
1.08E−02
D
5


LNU7_H4
0.83
2.75E−03
A
25
LNU219_H1
0.70
3.47E−02
D
5


LNU7_H4
0.80
5.94E−03
C
25
LNU219_H1
0.71
3.13E−02
D
1


LNU7_H4
0.71
2.13E−02
A
24
LNU7_H5
0.70
3.42E−02
D
1


LNU45_H9
0.78
7.80E−03
C
24
LNU7_H5
0.70
3.56E−02
D
1


LNU24_H2
0.90
4.44E−04
B
26
LNU219_H1
0.79
1.17E−02
D
8


LNU45_H11
0.90
4.44E−04
B
26
LNU7_H5
0.95
1.43E−02
B
35


LNU7_H4
0.73
1.61E−02
C
21
LNU45_H10
0.74
1.53E−02
C
12


LNU7_H4
0.75
1.18E−02
C
20
LNU76_H3
0.73
1.56E−02
C
9


LNU24_H2
0.76
1.07E−02
B
32
LNU74_H8
0.96
8.06E−03
C
18


LNU45_H11
0.76
1.07E−02
B
32
LNU74_H8
0.90
3.70E−02
C
18


LNU24_H2
0.86
1.39E−03
B
33
LNU76_H3
0.90
3.91E−02
C
18


LNU45_H11
0.86
1.39E−03
B
33
LNU45_H10
0.90
3.58E−02
C
18


LNU219_H1
0.87
1.13E−03
A
12
LNU45_H12
0.98
4.31E−03
C
18


LNU7_H4
0.82
3.80E−03
A
6
LNU256_H0
0.71
2.03E−02
C
11


LNU7_H4
0.78
7.67E−03
A
6
LNU76_H3
0.70
2.40E−02
C
11


LNU45_H10
0.89
5.71E−04
A
6
LNU46_H3
0.82
3.49E−03
C
6


LNU45_H9
0.73
1.76E−02
A
5
LNU45_H9
0.71
2.22E−02
C
5


LNU7_H4
0.74
1.45E−02
A
2
LNU45_H9
0.82
3.70E−03
C
2


LNU45_H10
0.86
1.29E−03
A
2
LNU7_H4
0.96
1.02E−02
B
35


LNU45_H9
0.80
5.07E−03
A
2
LNU219_H1
0.89
5.64E−04
C
8


LNU7_H4
0.70
2.32E−02
A
1
LNU74_H8
0.90
3.55E−02
B
35


LNU45_H10
0.89
5.60E−04
A
1
LNU45_H10
0.93
2.43E−02
B
35


LNU45_H9
0.74
1.49E−02
A
1
LNU45_H10
0.91
3.00E−02
B
35


LNU7_H4
0.98
2.32E−03
A
17
LNU46_H3
0.94
1.79E−02
B
35







LNU181_H0
0.95
1.14E−02
A
35





Table 7.


“Correl. Set ID”—correlation set ID according to the correlated parameters Table above.






Example 4
Production of Rice Transcriptom Using 44K Rice Oligonucleotide Micro-Array
In order to produce differential expression analysis of rice plants subjected to nitrogen limiting conditions compared to normal (non-limiting) nitrogen conditions, the present inventors have utilized a Rice oligonucleotide micro-array, produced by Agilent Technologies [Hypertext Transfer Protocol://World Wide Web (dot) chem. (dot) agilent (dot) com/Scripts/PDS (dot) asp?1 Page=50879]. The array oligonucleotide represents about 44,000 rice genes and transcripts.
Experimental Procedures
Rice plants grown under different nitrogen fertilization levels assessment—Five rice accessions were grown in 3 repetitive plots, each containing 10 plants, at a net house under semi-hydroponics conditions. Briefly, the growing protocol was as follows: Rice seeds were sown in trays filled with a mix of vermiculite and peat in a 1:1 ratio. Constant nitrogen limiting conditions were achieved by irrigating the plants with a solution containing 0.8 mM inorganic nitrogen in the form of KNO3, supplemented with 1 mM KH2PO4, 1 mM MgSO4, 3.6 mM K2SO4 and microelements, while normal nitrogen levels were achieved by applying a solution of 8 mM inorganic nitrogen also in the form of KNO3 with 1 mM KH2PO4, 1 mM MgSO4, and microelements.
Analyzed rice tissues—All 5 selected rice varieties were pooled in 1 batch per each treatment. Two tissues [leaves and roots] growing at two different nitrogen fertilization levels, 0.8 mM Nitrogen (nitrogen limiting conditions) or 8 mM Nitrogen (normal nitrogen conditions), were sampled and RNA was extracted as described above. For convenience, each micro-array expression information tissue type has received a Set ID as summarized in Table 8 below.







TABLE 8







Rice transcriptom experimental sets










Expression Set
Set ID







Leaves at 0.8 mM Nitrogen fertilization
A



Leaves at 8 mM Nitrogen fertilization
B



Roots at 0.8 mM Nitrogen fertilization
C



Roots at 8 mM Nitrogen fertilization
D







Table 8.






Experimental Results
Gene up-regulation under reduced nitrogen fertilization levels indicates the involvement of the genes in NUE improvement. LNU116, LNU117, LNU118, LNU119, LNU120, LNU216, LNU217 and LNU276 were upregulated in Set ID C compared to their expression level in Set ID D. In addition, LNU116, LNU121, LNU176, LNU216, LNU217, LNU276 were upregulated in Set ID A compared to their expression level in Set ID B.
Example 5
Production of Arabidopsis Transcriptom and High Throughput Correlation Analysis of Yield, Biomass and/or Vigor Related Parameters Using 44K Arabidopsis Full Genome Oligonucleotide Micro-Array
To produce a high throughput correlation analysis comparing between plant phenotype and gene expression level, the present inventors utilized an Arabidopsis thaliana oligonucleotide micro-array, produced by Agilent Technologies [Hypertext Transfer Protocol://World Wide Web (dot) chem. (dot) agilent (dot) com/Scripts/PDS (dot) asp?1 Page=50879]. The array oligonucleotide represents about 40,000 A. thaliana genes and transcripts designed based on data from the TIGR ATH1 v.5 database and Arabidopsis MPSS (University of Delaware) databases. To define correlations between the levels of RNA expression and yield, biomass components or vigor related parameters, various plant characteristics of 15 different Arabidopsis ecotypes were analyzed. Among them, nine ecotypes encompassing the observed variance were selected for RNA expression analysis. The correlation between the RNA levels and the characterized parameters was analyzed using Pearson correlation test [Hypertext Transfer Protocol://World Wide Web (dot) davidmlane (dot) com/hyperstat/A34739 (dot) html].
Experimental Procedures
Analyzed Arabidopsis tissues—Five tissues at different developmental stages including root, leaf, flower at anthesis, seed at 5 days after flowering (DAF) and seed at 12 DAF, representing different plant characteristics, were sampled and RNA was extracted as described above. Each micro-array expression information tissue type has received a Set ID as summarized in Table 9 below.







TABLE 9







Tissues used for Arabidopsis transcriptom expression sets










Expression Set
Set ID







Root
A



Leaf
B



Flower
C



Seed 5 DAF
D



Seed 12 DAF
E







Table 9: Provided are the identification (ID) letters of each of the Arabidopsis expression sets (A-E). DAF = days after flowering.






Yield components and vigor related parameters assessment—Eight out of the nine Arabidopsis ecotypes were used in each of 5 repetitive blocks (named A, B, C, D and E), each containing 20 plants per plot. The plants were grown in a greenhouse at controlled conditions in 22° C., and the N:P:K fertilizer (20:20:20; weight ratios) [nitrogen (N), phosphorus (P) and potassium (K)] was added. During this time data was collected, documented and analyzed. Additional data was collected through the seedling stage of plants grown in a tissue culture in vertical grown transparent agar plates. Most of chosen parameters were analyzed by digital imaging.
Digital imaging in Tissue culture—A laboratory image acquisition system was used for capturing images of plantlets sawn in square agar plates. The image acquisition system consists of a digital reflex camera (Canon EOS 300D) attached to a 55 mm focal length lens (Canon EF-S series), mounted on a reproduction device (Kaiser RS), which included 4 light units (4×150 Watts light bulb) and located in a darkroom.
Digital imaging in Greenhouse—The image capturing process was repeated every 3-4 days starting at day 7 till day 30. The same camera attached to a 24 mm focal length lens (Canon EF series), placed in a custom made iron mount, was used for capturing images of larger plants sawn in white tubs in an environmental controlled greenhouse. The white tubs were square shape with measurements of 36×26.2 cm and 7.5 cm deep. During the capture process, the tubs were placed beneath the iron mount, while avoiding direct sun light and casting of shadows. This process was repeated every 3-4 days for up to 30 days.
An image analysis system was used, which consists of a personal desktop computer (Intel P43.0 GHz processor) and a public domain program—ImageJ 1.37, Java based image processing program, which was developed at the U.S. National Institutes of Health and is freely available on the internet at Hypertext Transfer Protocol://rsbweb (dot) nih (dot) gov/. Images were captured in resolution of 6 Mega Pixels (3072×2048 pixels) and stored in a low compression JPEG (Joint Photographic Experts Group standard) format. Next, analyzed data was saved to text files and processed using the JMP statistical analysis software (SAS institute).
Leaf analysis—Using the digital analysis leaves data was calculated, including leaf number, area, perimeter, length and width. On day 30, 3-4 representative plants were chosen from each plot of blocks A, B and C. The plants were dissected, each leaf was separated and was introduced between two glass trays, a photo of each plant was taken and the various parameters (such as leaf total area, laminar length etc.) were calculated from the images. The blade circularity was calculated as laminar width divided by laminar length.
Root analysis—During 17 days, the different ecotypes were grown in transparent agar plates. The plates were photographed every 3 days starting at day 7 in the photography room and the roots development was documented (see examples in FIGS. 3A-F). The growth rate of roots was calculated according to Formula V.

Relative growth rate of root coverage=Regression coefficient of root coverage along time course.  Formula V:

Vegetative growth rate analysis—was calculated according to Formula VI. The analysis was ended with the appearance of overlapping plants.

Relative vegetative growth rate area=Regression coefficient of vegetative area along time course.  Formula VI

For comparison between ecotypes the calculated rate was normalized using plant developmental stage as represented by the number of true leaves. In cases where plants with 8 leaves had been sampled twice (for example at day 10 and day 13), only the largest sample was chosen and added to the Anova comparison.
Seeds in siliques analysis—On day 70, 15-17 siliques were collected from each plot in blocks D and E. The chosen siliques were light brown color but still intact. The siliques were opened in the photography room and the seeds were scatter on a glass tray, a high resolution digital picture was taken for each plot. Using the images the number of seeds per silique was determined.
Seeds average weight—At the end of the experiment all seeds from plots of blocks A-C were collected. An average weight of 0.02 grams was measured from each sample, the seeds were scattered on a glass tray and a picture was taken. Using the digital analysis, the number of seeds in each sample was calculated.
Oil percentage in seeds—At the end of the experiment all seeds from plots of blocks A-C were collected. Columbia seeds from 3 plots were mixed grounded and then mounted onto the extraction chamber. 210 ml of n-Hexane (Cat No. 080951 Biolab Ltd.) were used as the solvent. The extraction was performed for 30 hours at medium heat 50° C. Once the extraction has ended the n-Hexane was evaporated using the evaporator at 35° C. and vacuum conditions. The process was repeated twice. The information gained from the Soxhlet extractor (Soxhlet, F. Die gewichtsanalytische Bestimmung des Milchfettes, Polytechnisches J. (Dingler's) 1879, 232, 461) was used to create a calibration curve for the Low Resonance NMR. The content of oil of all seed samples was determined using the Low Resonance NMR (MARAN Ultra—Oxford Instrument) and its MultiQuant software package.
Silique length analysis—On day 50 from sowing, 30 siliques from different plants in each plot were sampled in block A. The chosen siliques were green-yellow in color and were collected from the bottom parts of a grown plant's stem. A digital photograph was taken to determine silique's length.
Dry weight and seed yield—On day 80 from sowing, the plants from blocks A-C were harvested and left to dry at 30° C. in a drying chamber. The biomass and seed weight of each plot was separated, measured and divided by the number of plants. Dry weight=total weight of the vegetative portion above ground (excluding roots) after drying at 30° C. in a drying chamber; Seed yield per plant=total seed weight per plant (gr).
Oil yield—The oil yield was calculated using Formula VII.

Seed Oil yield=Seed yield per plant (gr)*Oil % in seed.  Formula VII:

Harvest Index (seed)—The harvest index was calculated using Formula IV (described above).
Experimental Results
Nine different Arabidopsis ecotypes were grown and characterized for 18 parameters (named as vectors).







TABLE 10







Arabidopsis correlated parameters (vectors)











Correlation



Correlated parameter with
ID














Root length day 13 (cm)
1



Root length day 7 (cm)
2



Relative root growth (cm/day) day 13
3



Fresh weight per plant (gr) at
4



bolting stage



Dry matter per plant (gr)
5



Vegetative growth rate (cm2/day)
6



till 8 true leaves



Blade circularity
7



Lamina width (cm)
8



Lamina length (cm)
9



Total leaf area per plant (cm)
10



1000 Seed weight (gr)
11



Oil % per seed
12



Seeds per silique
13



Silique length (cm)
14



Seed yield per plant (gr)
15



Oil yield per plant (mg)
16



Harvest Index
17



Leaf width/length
18







Table 10. Provided are the Arabidopsis correlated parameters (correlation ID Nos. 1-18). Abbreviations: Cm = centimeter(s); gr = gram(s); mg = milligram(s).






The characterized values are summarized in Tables 11 and 12 below.







TABLE 11







Measured parameters in Arabidopsis ecotypes









Ecotype

















15
16
12
11
5
17
10
13
14




















An-1
0.34
118.63
34.42
0.0203
0.64
0.53
46.86
45.44
1.06


Col-0
0.44
138.73
31.19
0.0230
1.27
0.35
109.89
53.47
1.26


Ct-1
0.59
224.06
38.05
0.0252
1.05
0.56
58.36
58.47
1.31


Cvi (N8580)
0.42
116.26
27.76
0.0344
1.28
0.33
56.80
35.27
1.47


Gr-6
0.61
218.27
35.49
0.0202
1.69
0.37
114.66
48.56
1.24


Kondara
0.43
142.11
32.91
0.0263
1.34
0.32
110.82
37.00
1.09


Ler-1
0.36
114.15
31.56
0.0205
0.81
0.45
88.49
39.38
1.18


Mt-0
0.62
190.06
30.79
0.0226
1.21
0.51
121.79
40.53
1.18


Shakdara
0.55
187.62
34.02
0.0235
1.35
0.41
93.04
25.53
1.00





Table 11.


Provided are the values of each of the parameters measured in Arabidopsis ecotypes:


15 = Seed yield per plant (gram);


16 = oil yield per plant (mg);


12 = oil % per seed;


11 = 1000 seed weight (gr);


5 = dry matter per plant (gr);


17 = harvest index;


10 = total leaf area per plant (cm);


13 = seeds per silique;


14 = Silique length (cm).













TABLE 12







Additional measured parameters in Arabidopsis ecotypes









Ecotype

















6
3
2
1
4
9
8
18
7




















An-1
0.313
0.631
0.937
4.419
1.510
2.767
1.385
0.353
0.509


Col-0
0.378
0.664
1.759
8.530
3.607
3.544
1.697
0.288
0.481


Ct-1
0.484
1.176
0.701
5.621
1.935
3.274
1.460
0.316
0.450


Cvi (N8580)
0.474
1.089
0.728
4.834
2.082
3.785
1.374
0.258
0.370


Gr-6
0.425
0.907
0.991
5.957
3.556
3.690
1.828
0.356
0.501


Kondara
0.645
0.774
1.163
6.372
4.338
4.597
1.650
0.273
0.376


Ler-1
0.430
0.606
1.284
5.649
3.467
3.877
1.510
0.305
0.394


Mt-0
0.384
0.701
1.414
7.060
3.479
3.717
1.817
0.335
0.491


Shakdara
0.471
0.782
1.251
7.041
3.710
4.149
1.668
0.307
0.409





Table 12.


Provided are the values of each of the parameters measured in Arabidopsis ecotypes:


6 = Vegetative growth rate (cm2/day) until 8 true leaves;


3 = relative root growth (cm/day) (day 13);


2 = Root length day 7 (cm);


1 = Root length day 13 (cm);


4 = fresh weight per plant (gr) at bolting stage;


9. = Lamima length (cm);


8 = Lamina width (cm);


18 = Leaf width/length;


7 = Blade circularity.






Tables 13 and 14 provide the correlation analyses.







TABLE 13







Correlation between the expression level of selected LNU genes


of some embodiments of the invention in various tissues and


the phenotypic performance under normal or low nitrogen fertilization


conditions across Arabidopsis accessions













Gene


Exp.
Corr.



Name
R
P value
set
Set ID

















LNU1
0.74
3.45E−02
C
5



LNU1
0.83
2.02E−02
D
5



LNU1
0.81
2.58E−02
D
8



LNU1
0.77
4.08E−02
D
8



LNU1
0.97
4.59E−05
B
3



LNU1
0.88
4.21E−03
B
3



LNU1
0.73
3.79E−02
E
3



LNU1
0.92
1.10E−03
A
14



LNU1
0.72
4.38E−02
E
14



LNU123
0.87
4.82E−03
E
11



LNU123
0.82
1.22E−02
C
6



LNU124
0.73
3.90E−02
E
1



LNU124
0.71
4.96E−02
E
1



LNU125
0.79
2.07E−02
A
7



LNU125
0.72
4.19E−02
C
13



LNU125
0.85
7.49E−03
A
13



LNU126
0.80
3.09E−02
D
3



LNU127
0.82
2.36E−02
D
13



LNU127
0.81
1.53E−02
E
6



LNU129
0.74
3.42E−02
C
5



LNU129
0.85
1.49E−02
D
17



LNU129
0.79
2.04E−02
C
18



LNU129
0.73
3.85E−02
B
12



LNU129
0.77
2.60E−02
B
16



LNU129
0.86
5.89E−03
B
3



LNU129
0.82
1.25E−02
B
15



LNU129
0.78
2.34E−02
A
14



LNU132
0.82
1.26E−02
E
16



LNU132
0.94
6.45E−04
E
15



LNU133
0.80
3.06E−02
D
1



LNU133
0.91
4.56E−03
D
1



LNU134
0.78
2.26E−02
B
11



LNU134
0.75
3.13E−02
B
14



LNU135
0.76
4.61E−02
D
1



LNU135
0.84
8.88E−03
B
6



LNU135
0.74
3.45E−02
E
6



LNU136
0.82
1.18E−02
E
5



LNU136
0.79
1.85E−02
C
4



LNU136
0.76
2.70E−02
C
8



LNU136
0.71
4.69E−02
A
14



LNU136
0.82
1.18E−02
C
10



LNU14
0.72
4.41E−02
A
1



LNU14
0.86
6.57E−03
E
11



LNU14
0.75
3.20E−02
B
15



LNU14
0.75
3.11E−02
E
14



LNU140
0.71
4.67E−02
C
15



LNU15
0.75
3.37E−02
B
12



LNU15
0.95
2.59E−04
B
16



LNU15
0.91
1.48E−03
B
15



LNU170
0.85
7.15E−03
C
12



LNU170
0.76
3.00E−02
A
12



LNU170
0.75
3.30E−02
C
16



LNU170
0.92
1.24E−03
A
16



LNU170
0.79
1.86E−02
E
16



LNU170
0.76
2.96E−02
E
3



LNU170
0.87
4.80E−03
A
15



LNU170
0.73
3.82E−02
E
15



LNU175
0.81
1.49E−02
A
17



LNU175
0.76
4.68E−02
D
18



LNU177
0.90
5.47E−03
D
5



LNU177
0.96
4.41E−04
D
8



LNU177
0.71
4.99E−02
B
1



LNU177
0.90
5.34E−03
D
10



LNU178
0.82
1.26E−02
C
1



LNU178
0.71
4.72E−02
E
13



LNU179
0.83
1.14E−02
E
3



LNU179
0.73
4.13E−02
B
11



LNU179
0.78
2.32E−02
E
14



LNU179
0.84
9.15E−03
E
6



LNU181
0.75
3.03E−02
B
7



LNU181
0.77
2.41E−02
A
14



LNU183
0.80
1.68E−02
E
8



LNU183
0.77
2.68E−02
E
10



LNU184
0.73
3.79E−02
A
1



LNU185
0.83
2.23E−02
D
16



LNU185
0.77
4.27E−02
D
3



LNU185
0.92
1.20E−03
E
11



LNU185
0.75
3.22E−02
B
15



LNU185
0.82
2.27E−02
D
15



LNU186
0.79
1.88E−02
C
5



LNU186
0.81
1.57E−02
A
5



LNU186
0.80
1.59E−02
E
5



LNU186
0.73
4.09E−02
C
4



LNU186
0.80
1.77E−02
A
4



LNU186
0.74
3.39E−02
C
9



LNU186
0.75
3.24E−02
A
9



LNU186
0.73
3.92E−02
C
8



LNU186
0.82
1.17E−02
A
8



LNU186
0.83
1.14E−02
E
3



LNU186
0.71
4.90E−02
C
15



LNU186
0.79
2.05E−02
A
10



LNU186
0.72
4.50E−02
C
6



LNU187
0.81
1.38E−02
A
9



LNU187
0.77
2.40E−02
E
11



LNU206
0.76
2.89E−02
B
3



LNU207
0.83
1.08E−02
E
17



LNU210
0.74
3.52E−02
C
16



LNU210
0.89
2.97E−03
B
3



LNU210
0.74
3.61E−02
B
11



LNU211
0.92
1.22E−03
B
5



LNU211
0.73
4.00E−02
B
8



LNU212
0.85
7.78E−03
A
7



LNU212
0.75
3.23E−02
B
5



LNU212
0.72
4.52E−02
C
8



LNU212
0.93
9.64E−04
B
8



LNU212
0.77
2.59E−02
C
1



LNU212
0.77
2.54E−02
E
11



LNU212
0.77
2.63E−02
B
10



LNU213
0.72
4.28E−02
B
7



LNU213
0.86
6.08E−03
E
9



LNU213
0.75
3.39E−02
C
3



LNU213
0.88
3.77E−03
A
1



LNU213
0.81
1.40E−02
A
1



LNU213
0.85
1.66E−02
D
14



LNU213
0.83
1.14E−02
E
6



LNU214
0.73
4.08E−02
C
17



LNU215
0.86
6.04E−03
C
3



LNU215
0.77
2.68E−02
B
14



LNU215
0.78
2.15E−02
A
6



LNU218
0.81
1.53E−02
A
12



LNU218
0.73
3.81E−02
E
6



LNU219
0.71
4.78E−02
B
12



LNU219
0.78
2.20E−02
B
16



LNU219
0.71
4.98E−02
B
3



LNU225
0.75
3.36E−02
B
7



LNU225
0.81
1.58E−02
C
18



LNU225
0.90
2.55E−03
B
18



LNU225
0.83
1.03E−02
A
18



LNU225
0.75
3.27E−02
E
18



LNU23
0.90
2.05E−03
C
11



LNU23
0.91
1.72E−03
B
11



LNU234
0.71
4.90E−02
E
9



LNU234
0.75
3.19E−02
E
6



LNU24
0.73
4.05E−02
A
3



LNU247
0.81
1.51E−02
C
13



LNU249
0.74
3.73E−02
B
17



LNU249
0.77
4.42E−02
D
17



LNU249
0.75
3.35E−02
E
6



LNU250
0.74
3.45E−02
C
7



LNU250
0.74
3.67E−02
E
18



LNU250
0.71
4.84E−02
E
12



LNU250
0.78
2.20E−02
A
11



LNU251
0.79
3.56E−02
D
7



LNU251
0.85
8.12E−03
B
13



LNU251
0.73
3.88E−02
A
13



LNU251
0.78
2.12E−02
A
14



LNU254
0.83
9.96E−03
B
5



LNU254
0.78
2.20E−02
B
8



LNU255
0.89
2.76E−03
A
1



LNU256
0.79
1.85E−02
E
3



LNU256
0.89
2.98E−03
E
11



LNU256
0.73
4.18E−02
B
13



LNU256
0.78
2.10E−02
E
14



LNU258
0.75
3.08E−02
C
12



LNU258
0.81
1.50E−02
E
16



LNU258
0.83
9.93E−03
E
15



LNU258
0.81
1.48E−02
A
13



LNU260
0.85
1.52E−02
D
5



LNU260
0.79
3.43E−02
D
8



LNU260
0.74
3.40E−02
C
1



LNU260
0.73
3.78E−02
C
1



LNU260
0.83
1.10E−02
E
1



LNU261
0.73
3.90E−02
C
9



LNU261
0.76
2.98E−02
B
16



LNU261
0.85
6.86E−03
A
1



LNU261
0.82
2.29E−02
D
11



LNU261
0.94
1.95E−03
D
6



LNU262
0.77
4.15E−02
D
7



LNU262
0.71
4.97E−02
B
5



LNU262
0.71
4.67E−02
E
8



LNU262
0.87
5.11E−03
E
16



LNU262
0.94
4.36E−04
E
15



LNU8
0.74
3.71E−02
B
3



LNU8
0.84
8.67E−03
A
3



LNU8
0.72
4.37E−02
E
1



LNU8
0.72
4.38E−02
A
11







Table 13. “Correl. Set ID”—correlation set ID according to the correlated parameters Table above.













TABLE 14







Correlation between the expression level of selected


LNU orthologs genes of some embodiments of the invention


in various tissues and the phenotypic performance under


normal or low nitrogen fertilization conditions across


Arabidopsis accessions













Gene


Exp.
Correl.



Name
R
P value
Set
Set ID

















LNU24_H1
0.75
3.13E−02
C
7



LNU46_H3
0.75
3.24E−02
C
9



LNU181_H0
0.71
4.84E−02
C
3



LNU46_H5
0.87
4.72E−03
C
3



LNU76_H3
0.76
2.75E−02
C
2



LNU181_H0
0.75
3.05E−02
B
7



LNU256_H0
0.84
9.66E−03
B
5



LNU46_H4
0.73
3.78E−02
B
8



LNU45_H9
0.73
4.02E−02
B
16



LNU46_H4
0.76
2.91E−02
B
1



LNU24_H0
0.74
3.72E−02
B
11



LNU45_H9
0.77
2.55E−02
B
15



LNU181_H0
0.85
6.93E−03
B
13



LNU24_H0
0.73
3.87E−02
B
14



LNU76_H3
0.89
3.16E−03
B
14



LNU46_H3
0.73
3.97E−02
B
6



LNU256_H0
0.77
2.56E−02
A
5



LNU24_H1
0.71
4.63E−02
A
9



LNU256_H0
0.88
3.57E−03
A
9



LNU46_H4
0.85
8.11E−03
A
3



LNU24_H1
0.74
3.45E−02
A
11



LNU7_H4
0.71
4.64E−02
A
11



LNU181_H0
0.75
3.26E−02
A
14



LNU7_H5
0.80
1.74E−02
A
14



LNU45_H12
0.76
2.91E−02
A
14



LNU24_H1
0.80
1.77E−02
A
6



LNU256_H0
0.76
2.90E−02
A
6



LNU46_H3
0.72
4.43E−02
E
5



LNU219_H1
0.77
2.49E−02
E
8



LNU219_H1
0.80
1.69E−02
E
1



LNU219_H1
0.81
1.39E−02
E
2



LNU24_H0
0.86
5.99E−03
E
11



LNU74_H8
0.88
3.96E−03
E
11



LNU46_H4
0.76
2.94E−02
E
15



LNU181_H0
0.84
9.43E−03
E
14



LNU24_H0
0.83
1.09E−02
E
14



LNU74_H8
0.77
2.54E−02
E
14



LNU74_H9
0.76
2.93E−02
E
14



LNU46_H3
0.71
4.67E−02
E
14



LNU219_H1
0.81
1.43E−02
E
10



LNU76_H3
0.88
9.64E−03
D
5



LNU45_H10
0.89
6.71E−03
D
5



LNU76_H3
0.87
1.09E−02
D
8



LNU45_H10
0.95
9.46E−04
D
8



LNU46_H3
0.77
4.26E−02
D
8



LNU45_H9
0.88
9.11E−03
D
2



LNU74_H9
0.85
1.63E−02
D
11



LNU45_H10
0.83
2.05E−02
D
10



LNU45_H12
0.76
4.62E−02
D
6







Table 14. “Correl. Set ID”—correlation set ID according to the correlated parameters Table above.






Example 6
Production of Barley Transcriptom and High Throughput Correlation Analysis Using 44K Barley Oligonucleotide Micro-Array
In order to produce a high throughput correlation analysis comparing between plant phenotype and gene expression level, the present inventors utilized a Barley oligonucleotide micro-array, produced by Agilent Technologies [Hypertext Transfer Protocol://World Wide Web (dot) chem. (dot) agilent (dot) com/Scripts/PDS (dot) asp?1 Page=50879]. The array oligonucleotide represents about 47,500 Barley genes and transcripts. In order to define correlations between the levels of RNA expression and yield or vigor related parameters, various plant characteristics of 25 different Barley accessions were analyzed. Among them, 13 accessions encompassing the observed variance were selected for RNA expression analysis. The correlation between the RNA levels and the characterized parameters was analyzed using Pearson correlation test [Hypertext Transfer Protocol://World Wide Web (dot) davidmlane (dot) com/hyperstat/A34739 (dot) html].
Experimental Procedures
Analyzed Barley tissues—Five tissues at different developmental stages [meristem, flower, booting spike, stem, flag leaf], representing different plant characteristics, were sampled and RNA was extracted as described above. Each micro-array expression information tissue type has received a Set ID as summarized in Table 15 below.







TABLE 15







Barley transcriptom expression sets










Expression Set
Set ID







Meristem
A



Flower
B



Booting spike
C



Stem
D



Flag leaf
E







Table 15






Barley yield components and vigor related parameters assessment—25 Barley accessions in 4 repetitive blocks (named A, B, C, and D), each containing 4 plants per plot were grown at net house. Plants were phenotyped on a daily basis following the standard descriptor of barley (Table 16, below). Harvest was conducted while 50% of the spikes were dry to avoid spontaneous release of the seeds. Plants were separated to the vegetative part and spikes, of them, 5 spikes were threshed (grains were separated from the glumes) for additional grain analysis such as size measurement, grain count per spike and grain yield per spike. All material was oven dried and the seeds were threshed manually from the spikes prior to measurement of the seed characteristics (weight and size) using scanning and image analysis. The image analysis system included a personal desktop computer (Intel P4 3.0 GHz processor) and a public domain program—ImageJ 1.37 (Java based image processing program, which was developed at the U.S. National Institutes of Health and freely available on the internet [Hypertext Transfer Protocol://rsbweb (dot) nih (dot) gov/]. Next, analyzed data was saved to text files and processed using the JMP statistical analysis software (SAS institute).







TABLE 16







Barley standard descriptors










Trait
Parameter
Range
Description





Growth habit
Scoring
1-9
Prostrate (1) or Erect (9)


Hairiness of
Scoring
P (Presence)/
Absence (1) or Presence (2)


basal leaves

A (Absence)


Stem
Scoring
1-5
Green (1), Basal only or


pigmentation


Half or more (5)


Days to
Days

Days from sowing to


Flowering


emergence of awns


Plant height
Centimeter

Height from ground level



(cm)

to top of the longest spike





excluding awns


Spikes per
Number

Terminal Counting


plant


Spike length
Centimeter

Terminal Counting 5 spikes



(cm)

per plant


Grains per
Number

Terminal Counting 5 spikes


spike


per plant


Vegetative dry
Gram

Oven-dried for 48 hours at


weight


70° C.


Spikes dry
Gram

Oven-dried for 48 hours at


weight


30° C.





Table 16.






Grains per spike—At the end of the experiment (50% of the spikes were dry) all spikes from plots within blocks A-D are collected. The total number of grains from 5 spikes that were manually threshed was counted. The average grain per spike is calculated by dividing the total grain number by the number of spikes.
Grain average size (cm)—At the end of the experiment (50% of the spikes were dry) all spikes from plots within blocks A-D are collected. The total grains from 5 spikes that were manually threshed were scanned and images were analyzed using the digital imaging system. Grain scanning was done using Brother scanner (model DCP-135), at the 200 dpi resolution and analyzed with Image J software. The average grain size was calculated by dividing the total grain size by the total grain number.
Grain average weight (mgr)—At the end of the experiment (50% of the spikes were dry) all spikes from plots within blocks A-D are collected. The total grains from 5 spikes that were manually threshed were counted and weight. The average weight was calculated by dividing the total weight by the total grain number.
Grain yield per spike (gr)—At the end of the experiment (50% of the spikes were dry) all spikes from plots within blocks A-D are collected. The total grains from 5 spikes that were manually threshed were weight. The grain yield was calculated by dividing the total weight by the spike number.
Spike length analysis—At the end of the experiment (50% of the spikes were dry) all spikes from plots within blocks A-D are collected. The five chosen spikes per plant were measured using measuring tape excluding the awns.
Spike number analysis—At the end of the experiment (50% of the spikes were dry) all spikes from plots within blocks A-D are collected. The spikes per plant were counted.
Growth habit scoring—At the growth stage 10 (booting), each of the plants was scored for its growth habit nature. The scale that was used was 1 for prostate nature till 9 for erect.
Hairiness of basal leaves—At the growth stage 5 (leaf sheath strongly erect; end of tillering), each of the plants was scored for its hairiness nature of the leaf before the last. The scale that was used was 1 for prostate nature till 9 for erect.

    
    
        Plant height—At the harvest stage (50% of spikes were dry) each of the plants was measured for its height using measuring tape. Height was measured from ground level to top of the longest spike excluding awns.
    
    


Days to flowering—Each of the plants was monitored for flowering date. Days of flowering was calculated from sowing date till flowering date.
Stem pigmentation—At the growth stage 10 (booting), each of the plants was scored for its stem color. The scale that was used was 1 for green till 5 for full purple.
Vegetative dry weight and spike yield—At the end of the experiment (50% of the spikes were dry) all spikes and vegetative material from plots within blocks A-D are collected. The biomass and spikes weight of each plot was separated, measured and divided by the number of plants.
Dry weight=total weight of the vegetative portion above ground (excluding roots) after drying at 70° C. in oven for 48 hours;
Spike yield per plant=total spike weight per plant (gr) after drying at 30° C. in oven for 48 hours.
Harvest Index (for barley)—The harvest index is calculated using Formula VIII.

Harvest Index=Average spike dry weight per plant/(Average vegetative dry weight per plant+Average spike dry weight per plant)  Formula VIII:








TABLE 17







Barley correlated parameters (vectors)










Correlated parameter with (units)
Correlation Id














Grains per spike (numbers)
1



Grains size (mm2)
2



Grain weight (miligrams)
3



Grain Yield per spike (gr/spike)
4



Spike length (cm)
5



Spikes per plant (numbers)
6



Growth habit (scores 1-9)
7



Hairiness of basal leaves (scoring 1-2)
8



Plant height (cm)
9



Days to flowering (days)
10



Stem pigmentation (scoring 1-5)
11



Vegetative dry weight (gram)
12



Harvest Index (ratio)
13







Table 17.






Experimental Results
13 different Barley accessions were grown and characterized for 13 parameters as described above. The average for each of the measured parameter was calculated using the JMP software and values are summarized in Tables 18 and 19 below. Subsequent correlation analysis between the various transcriptom sets (Table 15) and the average parameters, was conducted (Tables 20 and 21). Follow, results were integrated to the database.







TABLE 18







Measured parameters of correlation Ids in Barley accessions









Parameter














Accession
6
10
3
5
2
1
7

















Amatzya
48.85
62.40
35.05
12.04
0.27
20.23
2.60


Ashqelon
48.27
64.08
28.06
10.93
0.23
17.98
2.00


Canada park
37.42
65.15
28.76
11.83
0.24
17.27
1.92


Havarim stream
61.92
58.92
17.87
9.90
0.17
17.73
3.17


Jordan est
33.27
63.00
41.22
11.68
0.29
14.47
4.33


Klil
41.69
70.54
29.73
11.53
0.28
16.78
2.69


Maale Efraim
ND
52.80
25.22
8.86
0.22
13.47
3.60


Mt Arbel
40.63
60.88
34.99
11.22
0.28
14.07
3.50


Mt Harif
62.00
58.10
20.58
11.11
0.19
21.54
3.00


Neomi
49.33
53.00
27.50
8.58
0.22
12.10
3.67


Neot Kdumim
50.60
60.40
37.13
10.18
0.27
14.36
2.47


Oren canyon
43.09
64.58
29.56
10.51
0.27
15.28
3.50


Yeruham
51.40
56.00
19.58
9.80
0.18
17.07
3.00





Table 18. Provided are the values of each of the parameters measured in Barley accessions according to the following correlation identifications (Correlation Ids): 6 = Spikes per plant; 10 = Days to flowering; 3 = Grain weight; 5 = Spike length; 2 = Grains Size; 1 = Grains per spike; 7 = Growth habit.













TABLE 19







Barley accessions, additional measured parameters









Parameter













Accession
8
9
4
11
12
13
















Amatzya
1.53
134.27
3.56
1.13
78.87
0.45


Ashqelon
1.33
130.50
2.54
2.50
66.14
0.42


Canada park
1.69
138.77
2.58
1.69
68.49
0.40


Havarim stream
1.08
114.58
1.57
1.75
53.39
0.44


Jordan est
1.42
127.75
3.03
2.33
68.30
0.43


Klil
1.69
129.38
2.52
2.31
74.17
0.40


Maale Efraim
1.30
103.89
1.55
1.70
35.35
0.52


Mt Arbel
1.19
121.63
2.62
2.19
58.33
0.48


Mt Harif
1.00
126.80
2.30
2.30
62.23
0.44


Neomi
1.17
99.83
1.68
1.83
38.32
0.49


Neot Kdumim
1.60
121.40
2.68
3.07
68.31
0.45


Oren canyon
1.08
118.42
2.35
1.58
56.15
ND


Yeruham
1.17
117.17
1.67
2.17
42.68
ND





Table 19. Provided are the values of each of the parameters measured in Barley accessions according to the following correlation identifications (Correlation Ids): 8 = Hairiness of basal leaves; 9 = Plant height; 4 = Grain yield per spike; 11 = Stem pigmentation; 12 = Vegetative dry weight; 13 = Harvest Index.













TABLE 20







Correlation between the expression level of selected


LNU genes of some embodiments of the invention in various


tissues and the phenotypic performance under normal fertilization


conditions across barley accessions













Gene


Exp.
Corr.



Name
R
P value
Set ID
Set ID

















LNU72
0.77
6.01E−03
A
2



LNU72
0.75
7.97E−03
A
3



LNU72
0.75
2.03E−02
A
3



LNU72
0.74
2.13E−02
A
2



LNU240
0.73
1.15E−02
C
6



LNU240
0.70
3.44E−02
C
6



LNU244
0.72
4.57E−02
C
7



LNU27
0.81
7.70E−03
A
1



LNU27
0.78
1.24E−02
A
9



LNU27
0.76
1.72E−02
A
12



LNU27
0.72
1.17E−02
A
12



LNU28
0.80
1.02E−02
C
2



LNU28
0.79
1.14E−02
C
3



LNU28
0.78
4.43E−03
C
2



LNU28
0.78
4.67E−03
C
3



LNU172
0.81
1.56E−02
C
7



LNU228
0.86
3.07E−03
A
3



LNU228
0.86
7.60E−04
A
3



LNU228
0.81
7.47E−03
A
2



LNU228
0.79
1.99E−02
C
6



LNU228
0.79
3.90E−03
C
6



LNU228
0.75
7.78E−03
A
2



LNU224
0.85
1.53E−02
C
6







Table 20. “Correl. Set ID”—correlation set ID according to the correlated parameters Table above.













TABLE 21







Correlation between the expression level of selected LNU


orthologs genes of some embodiments of the invention in


various tissues and the phenotypic performance under normal


fertilization conditions across barley accessions













Gene


Exp.
Correl.



Name
R
P value
Set
Set ID







LNU52_H0
0.91
5.92E−04
C
3



LNU52_H0
0.91
9.60E−05
C
3



LNU89_H0
0.76
2.80E−02
C
3



LNU89_H0
0.76
2.80E−02
C
3



LNU85_H0
0.74
2.27E−02
C
3



LNU85_H0
0.74
2.27E−02
C
3



LNU2_H0
0.75
1.94E−02
C
1



LNU2_H0
0.75
1.94E−02
C
1



LNU2_H0
0.73
2.65E−02
C
1



LNU2_H0
0.72
1.30E−02
C
1



LNU2_H0
0.73
2.65E−02
C
1



LNU2_H0
0.72
1.30E−02
C
1



LNU268_H0
0.79
4.16E−03
C
1



LNU268_H0
0.78
1.27E−02
C
1



LNU52_H0
0.93
2.81E−04
C
2



LNU52_H0
0.92
5.39E−05
C
2



LNU85_H0
0.73
2.63E−02
B
2



LNU85_H0
0.73
2.63E−02
B
2



LNU89_H0
0.81
1.44E−02
B
2



LNU89_H0
0.81
1.44E−02
B
2



LNU85_H0
0.74
2.21E−02
B
2



LNU85_H0
0.71
1.51E−02
B
2



LNU85_H0
0.74
2.21E−02
B
2



LNU85_H0
0.71
1.51E−02
B
2



LNU74_H31
0.72
3.00E−02
B
7



LNU74_H31
0.72
3.00E−02
B
7



LNU85_H0
0.83
5.97E−03
B
8



LNU85_H0
0.81
2.45E−03
B
8



LNU85_H0
0.83
5.97E−03
A
8



LNU85_H0
0.81
2.45E−03
A
8



LNU52_H1
0.71
4.86E−02
A
8



LNU52_H1
0.70
2.31E−02
A
8



LNU52_H1
0.71
4.86E−02
A
8



LNU52_H1
0.70
2.31E−02
A
8



LNU89_H0
0.73
3.99E−02
A
8



LNU89_H0
0.73
3.99E−02
A
8



LNU76_H11
0.71
3.36E−02
A
8



LNU76_H11
0.71
3.36E−02
A
8



LNU2_H0
0.81
8.66E−03
A
9



LNU2_H0
0.72
1.31E−02
A
9



LNU2_H0
0.81
8.66E−03
A
9



LNU2_H0
0.72
1.31E−02
A
9



LNU2_H0
0.88
1.59E−03
A
5



LNU2_H0
0.75
7.76E−03
A
5



LNU2_H0
0.88
1.59E−03
A
5



LNU2_H0
0.75
7.76E−03
A
5



LNU69_H0
0.79
2.01E−02
A
6



LNU69_H0
0.79
2.01E−02
A
6



LNU45_H52
0.76
4.62E−02
A
6



LNU45_H52
0.76
4.62E−02
A
6



LNU51_H0
0.86
6.78E−03
A
6



LNU51_H0
0.71
1.41E−02
A
6



LNU51_H0
0.86
6.78E−03
A
6



LNU51_H0
0.71
1.41E−02
A
6



LNU60_H0
0.72
1.31E−02
A
6



LNU60_H0
0.72
1.31E−02
A
6



LNU67_H0
0.88
3.84E−03
A
6



LNU67_H0
0.72
1.17E−02
A
6



LNU67_H0
0.88
3.84E−03
A
6



LNU67_H0
0.72
1.17E−02
A
6



LNU46_H7
0.89
3.10E−03
A
6



LNU46_H7
0.83
1.45E−03
A
6



LNU46_H7
0.89
3.10E−03
A
6



LNU46_H7
0.83
1.45E−03
A
6



LNU35_H0
0.87
4.90E−03
A
6



LNU35_H0
0.85
8.62E−04
A
6



LNU35_H0
0.87
4.90E−03
A
6



LNU35_H0
0.85
8.62E−04
A
6







Table 21. “Correl. Set ID”—correlation set ID according to the correlated parameters Table above.






Example 7
Production of Sorghum Transcriptom and High Throughput Correlation Analysis with Yield, NUE, and ABST Related Parameters Measured in Fields Using 44K Sorguhm Oligonucleotide Micro-Arrays
In order to produce a high throughput correlation analysis between plant phenotype and gene expression level, the present inventors utilized a sorghum oligonucleotide micro-array, produced by Agilent Technologies [Hypertext Transfer Protocol://World Wide Web (dot) chem. (dot) agilent (dot) com/Scripts/PDS (dot) asp?1 Page=50879]. The array oligonucleotide represents about 44,000 sorghum genes and transcripts. In order to define correlations between the levels of RNA expression with ABST, yield and NUE components or vigor related parameters, various plant characteristics of 17 different sorghum hybrids were analyzed. Among them, 10 hybrids encompassing the observed variance were selected for RNA expression analysis. The correlation between the RNA levels and the characterized parameters was analyzed using Pearson correlation test [Hypertext Transfer Protocol://World Wide Web (dot) davidmlane (dot) com/hyperstat/A34739 (dot) html].
Correlation of Sorghum Varieties Across Ecotypes Grown Under Low Nitrogen, Regular Growth and Severe Drought Conditions
Experimental Procedures
17 Sorghum varieties were grown in 3 repetitive plots, in field. Briefly, the growing protocol was as follows:
1. Regular growth conditions: sorghum plants were grown in the field using commercial fertilization and irrigation protocols.
2. Low Nitrogen fertilization conditions: sorghum plants were fertilized with 50% less amount of nitrogen in the field than the amount of nitrogen applied in the regular growth treatment. All the fertilizer was applied before flowering.
3. Drought stress: sorghum seeds were sown in soil and grown under normal condition until around 35 days from sowing, around V8. At this point, irrigation was stopped, and severe drought stress was developed. In order to define correlations between the levels of RNA expression with NUE, drought, and yield components or vigor related parameters, the 17 different sorghum varieties were analyzed. Among them, 10 varieties encompassing the observed variance were selected for RNA expression analysis. The correlation between the RNA levels and the characterized parameters was analyzed using Pearson correlation test [Hypertext Transfer Protocol://World Wide Web (dot) davidmlane (dot) com/hyperstat/A34739 (dot) html].
Analyzed Sorghum tissues—All 10 selected Sorghum hybrids were sample per each treatment. Plant tissues [Flag leaf, Flower meristem and Flower] growing under low nitrogen, severe drought stress and plants grown under Normal conditions were sampled and RNA was extracted as described above. Each micro-array expression information tissue type has received a Set ID as summarized in Table 22 below.







TABLE 22







Sorghum transcriptom expression sets in field experiments










Expression Set
Set ID







Sorghum field/Normal/flower meristem
A



Sorghum field/Normal/flower
B



Sorghum field/Normal/leaf
C



Sorghum field/Low N/flower meristem
D



Sorghum field/Low N/flower
E



Sorghum field/Low N/leaf
F



Sorghum field/Drought/flower meristem
G



Sorghum field/Drought/flower
H



Sorghum field/Drought/leaf
I







Table 22: Provided are the sorghum transcriptom expression sets.






The following parameters were collected using digital imaging system:
Average Grain Area (cm2)—At the end of the growing period the grains were separated from the Plant ‘Head’. A sample of  ̃200 grains were weight, photographed and images were processed using the below described image processing system. The grain area was measured from those images and was divided by the number of grains.
Average Grain Length (cm)—At the end of the growing period the grains were separated from the Plant ‘Head’. A sample of  ̃200 grains were weight, photographed and images were processed using the below described image processing system. The sum of grain lengths (longest axis) was measured from those images and was divided by the number of grains.
Head Average Area (cm2) At the end of the growing period 5 ‘Heads’ were, photographed and images were processed using the below described image processing system. The ‘Head’ area was measured from those images and was divided by the number of ‘Heads’.
Head Average Length (cm) At the end of the growing period 5 ‘Heads’ were, photographed and images were processed using the below described image processing system. The ‘Head’ length (longest axis) was measured from those images and was divided by the number of ‘Heads’.
The image processing system was used, which consists of a personal desktop computer (Intel P4 3.0 GHz processor) and a public domain program—ImageJ 1.37, Java based image processing software, which was developed at the U.S. National Institutes of Health and is freely available on the internet at Hypertext Transfer Protocol://rsbweb (dot) nih (dot) gov/. Images were captured in resolution of 10 Mega Pixels (3888×2592 pixels) and stored in a low compression JPEG (Joint Photographic Experts Group standard) format. Next, image processing output data for seed area and seed length was saved to text files and analyzed using the JMP statistical analysis software (SAS institute).
Additional parameters were collected either by sampling 5 plants per plot or by measuring the parameter across all the plants within the plot.
Total Seed Weight per Head (gr.)—At the end of the experiment (plant ‘Heads’) heads from plots within blocks A-C were collected. 5 heads were separately threshed and grains were weighted, all additional heads were threshed together and weighted as well. The average grain weight per head was calculated by dividing the total grain weight by number of total heads per plot (based on plot). In case of 5 heads, the total grains weight of 5 heads was divided by 5.
FW Head per Plant gr—At the end of the experiment (when heads were harvested) total and 5 selected heads per plots within blocks A-C were collected separately. The heads (total and 5) were weighted (gr.) separately and the average fresh weight per plant was calculated for total (FW Head/Plant gr based on plot) and for 5 (FW Head/Plant gr based on 5 plants).
Plant height—Plants were characterized for height during growing period at 5 time points. In each measure, plants were measured for their height using a measuring tape. Height was measured from ground level to top of the longest leaf.
Plant leaf number—Plants were characterized for leaf number during growing period at 5 time points. In each measure, plants were measured for their leaf number by counting all the leaves of 3 selected plants per plot.
Relative Growth Rate was calculated using Formulas IX and X.

Relative growth rate of plant height=Regression coefficient of plant height along time course.  Formula IX

Relative growth rate of plant leaf number=Regression coefficient of plant leaf number along time course.  Formula X

SPAD—Chlorophyll content was determined using a Minolta SPAD 502 chlorophyll meter and measurement was performed 64 days post sowing. SPAD meter readings were done on young fully developed leaf. Three measurements per leaf were taken per plot.
Vegetative dry weight and Heads—At the end of the experiment (when Inflorescence were dry) all Inflorescence and vegetative material from plots within blocks A-C were collected. The biomass and Heads weight of each plot was separated, measured and divided by the number of Heads.
Dry weight=total weight of the vegetative portion above ground (excluding roots) after drying at 70° C. in oven for 48 hours;
Harvest Index (HI) (Sorghum)—The harvest index was calculated using Formula XI.

Harvest Index=Average grain dry weight per Head/(Average vegetative dry weight per Head+Average Head dry weight)  Formula XI:

FW Heads/(FW Heads+FW Plants)—The total fresh weight of heads and their respective plant biomass were measured at the harvest day. The heads weight was divided by the sum of weights of heads and plants.
Experimental Results
17 different sorghum hybrids were grown and characterized for different parameters: The average for each of the measured parameter was calculated using the JMP software (Tables 23-29) and a subsequent correlation analysis was performed (Tables 30-31). Results were then integrated to the database.







TABLE 23







Sorghum correlated parameters (vectors)








Correlation set
Correlation ID











Leaf SPAD 64 Days Post Sowing-normal [SPAD unit]
1


RGR of Leaf Num-normal
2


Total Seed Weight/Head gr based on plot-normal [gr]
3


Head Average Area cm2-normal [cm2]
4


Head Average Length cm-normal [cm]
5


Average Seed Area cm2-normal [cm2]
6


Average Seed Length cm-normal [cm]
7


FW Head/Plant gr based on plot-normal [gr]
8


FW per Plant gr based on plot-normal [gr]
9


Final Plant Height cm-normal [cm]
10


HI-normal
11


FW Heads/(FW Heads + FW Plants) all plot-normal [gr]
12


RGR of Plant Height-normal
13


FW-Inflorescence per Plant Normal [gr]
14


DW per Plant Normal [gr]
15


DW-5 Inflorescence Normal [gr]
16


Seed Yield Normal [gr]
17


Leaf No 2 Normal [number]
18


Plant Height 2 Normal [cm]
19


SPAD 2 Normal [SPAD unit]
20


Leaf No 3 Normal [number]
21


Plant Height 3 Normal [cm]
22


Leaf No 4 Normal [number]
23


Plant Height 4 Normal [cm]
24


Leaf No 5 Normal [number]
25


Leaf No 6 Normal [number]
26


Plant Height 6 Normal [cm]
27


Plant Height 5 Low-N [cm]
28


FW-Inflorescence per Plant Low-N
29


DW per Plant Low-N
30


Seed per Plant Low-N
31


Seed yield Low-N [gr]
32


Leaf No 2 Low-N [number]
33


Plant Height 2 Low-N [cm]
34


SPAD 2 Low-N
35


Leaf No 3 Low-N [number]
36


Plant Height 3 Low-N [cm]
37


Leaf No 5 Low-N [number]
38


RGR of Leaf Num-NUE
39


Total Seed Yield per Head gr based on plot-NUE
40


Head Average Area cm2-NUE [cm2]
41


Head Average Perimeter cm-NUE [cm]
42


Head Average Length cm-NUE [cm]
43


Head Average Width cm-NUE [cm]
44


Average Seed Area cm2-NUE [cm2]
45


Average Seed Length cm-NUE [cm]
46


FW Head per Plant gr based on plot-NUE [gr]
47


FW per Plant gr based on plot-NUE [gr]
48


Leaf SPAD 64 Days Post Sowing-NUE [SPAD unit]
49


HI-NUE
50


FW Heads/(FW Heads + FW Plants) all plot-NUE [gr]
51


FW-Inflorescence per Plant Drought [gr]
52


Dw per Plant Drought [gr]
53


Seed per Plant Drought [gr]
54


DW-5 Inflorescence Drought [gr]
55


Seed Yield Drought [gr]
56


Seed Yield (5 heads) gr Drought
57


Leaf No 2 Drought [number]
58


Plant Height 2 Drought [cm]
59


SPAD 2 Drought [SPAD unit]
60


Leaf No 3 Drought [number]
61


Plant Height 3 Drought [cm]
62


Leaf No 4 Drought [number]
63


Plant Height 4 Drought [cm]
64


Leaf No 5 Drought [number]
65


Plant Height 5 Drought [cm]
66


Leaf No 6 Drought [number]
67


Plant Height 6 Drought [cm]
68


Average Seed Area cm2-Drought [cm2]
69


Average Seed Length cm-Drought [cm]
70


Total Seed Yield per Head gr based on plot-Drought [gr]
71


Head Average Area cm2-Drought [cm2]
72


Head Average Perimeter cm-Drought [cm]
73


Head Average Length cm-Drought [cm]
74


Head Average Width cm-Drought [cm]
75


HI-Drought
76


RGR of Leaf_Num-Drought
77





Table 23. Provided are the Sorghum correlated parameters (vectors).


“gr.” = grams;


“SPAD” = chlorophyll levels;


“FW” = Plant Fresh weight;


“DW” = Plant Dry weight;


“normal” = standard growth conditions.













TABLE 24







Measured parameters in Sorghum accessions under normal conditions





















Seed ID
1
2
3
4
5
6
7
8
9
10
11
12
13
14
























20
43
0.10
31
120
26
0.11
0.39
175
163
95
201
0.51
1.89
0.18


21


26
168
27
0.11
0.40
223
213
79
127
0.51
1.62
0.68


22
43
0.21
19
85
21
0.13
0.45
56
335
198
52
0.12
3.42
0.06


24
45
0.19
38
157
27
0.13
0.45
112
313
234
122
0.26
2.42
0.11


25
46
0.19







189
55
0.12
3.12
0.07


26
42
0.16







195
94
0.18
3.32
0.09


27
45
0.20
48
169
31
0.11
0.40
126
151
117
327
0.46
2.18
0.13


28
45
0.17
31
109
23
0.11
0.41
108
138
93
231
0.43
2.19
0.11


29
43
0.18
40
135
26
0.10
0.38
124
168
113
241
0.43
2.57
0.12


30
46

38
169
29
0.12
0.42
103
129
98
304
0.44
2.05
0.10


31
45

32
156
28
0.12
0.43
82
98
98
336
0.46
2.07
0.08


32
45
0.18
33
112
23
0.11
0.40
78
99
100
350
0.45
2.55
0.08


33
47
0.12
33
155
28
0.12
0.41
91
112
106
293
0.45
2.33
0.09


34
44
0.21
52
172
30
0.11
0.40
150
157
151
411
0.51
3.04
0.15


35
45
0.19
36
169
31
0.11
0.40
109
131
117
285
0.46
2.33
0.11


36
45
0.15
38
163
27
0.11
0.40
108
136
124
283
0.44
2.52
0.11


37
43
0.24
42
170
29
0.11
0.39
131
209
126
204
0.39
2.81
0.13





Table 24: Provided are the values of each of the parameters (as described above) measured in Sorghum accessions (Seed ID) under normal conditions. Growth conditions are specified in the experimental procedure section.













TABLE 25







Additional measured parameters in Sorghum accessions under normal growth conditions




















Seed ID
15
16
17
18
19
20
21
22
23
24
25
26
27























20
0.16
0.04
1.15
5.31
10.6
43
5.44
24.5
6.73
37.3
6.75
6.73
37.3


21
0.25
0.06
0.48
6.08
8
40.7
6.38
19.9
8.44
47.8
8.38
8.44
47.8


22
0.34
0.03
1.22
6
16
43.3
6.56
31.1
7.25
40.1
9.81
7.25
40.1


24
0.31
0.03
2.61
5.44
11.9
44.7
5.75
24.5
7.88
45.9
8.81
7.88
45.9


25
0.46
0.07
0.99
5.38
14.2
45.8
5.12
27.2
8.12
41.4
8.69
8.12
41.4


26
0.36
0.05
2.67
6.31
15.3
41.6
6.31
27.2
9.12
44.9
9.5
9.12
44.9


27
0.15
0.05
3.59
6.12
15.7
45.2
6.19
31.2
7.5
42.1
9.19
7.5
42.1


28
0.14
0.03
2.12
6.06
15.9
45.1
6.06
30.2
8.25
41
6.69
8.25
41


29
0.17
0.05
2.50
6.12
14.6
43
5.62
26.2
7
42.5
9
7
42.1


30
0.13
0.04
2.78
6.44
18.4
45.6
5.94
31.9
6.75
41.9
6.75
6.75
41.9


31
0.10
0.02
3.16
7
18.1
44.8
6
32.2
8.06
43.4
6.38
8.06
43.4


32
0.10
0.04
3.41
6.31
17.2
45.3
6.38
29.8
6.94
39.8
7.81
6.94
39.8


33
0.11
0.05
2.94
6.25
14.9
46.5
6.31
28.1
7.94
40.6
7.88
7.94
40.6


34
0.16
0.05
2.80
6.25
13.5
44
5.94
27
8.06
44.4
9.94
8.06
44.4


35
0.13
0.04
2.90
5.62
14.6
45.1
5.94
29.2
7.56
43.2
8.69
7.56
43.2


36
0.14
0.04
3.40
6.38
16.4
45.1
5.75
27.8
7.94
41
8.56
7.94
41


37
0.21
0.06
3.16
5.88
17.3
43.1
6.56
31.6
10.3





Table 25: Provided are the values of each of the parameters (as described above) measured in Sorghum accessions (Seed ID) under normal conditions. Growth conditions are specified in the experimental procedure section.













TABLE 26







Measured parameters in Sorghum accessions under Low nitrogen conditions




















Seed Id
28
29
30
31
32
33
34
35
36
37
38
39
40























20
52.2
0.155
0.208
0.0221
0.563
5.47
12
40.6
6
27.8
7.67

25.9


21
40.6
0.122
0.138
0.0168
0.347
5.58
9.58
40.9
6.33
22.7
8.5
0.185
30.6


22
67.4
0.131
0.255
0.00919
0.68
5.58
13.9
45
5.83
27.8
8.83
0.186
19.4


24
66.2
0.241
0.402
0.104
0.94
5.25
10.6
42.3
5.25
24.1
8.83
0.219
35.6


25
68.1
0.069
0.234
0.00324
0.055
5
14.5
45.2
5.17
30.2
8.75
0.23
25.2


26
77.7
0.186
0.392
0.022
1.63
5.67
15.7
40.6
6.83
31.5
10.2
0.197
22.2


27
54.3
0.0621
0.0893
0.00997
0.755
5.5
13.8
44.8
6.67
32.3
8.83
0.181
50


28
71.8
0.039
0.0506
0.0186
0.863
5.58
15
45.1
6.25
29.9
7.33
0.213
27.5


29
81.6
0.0589
0.087
0.0293
1.45
6
17.5
40.6
6.25
33.2
8.58

51.1


30
57.6
0.0764
0.12
0.0105
1.06
6.17
19.4
45.4
6.25
34.6
6.42

36.8


31
61.5
0.0335
0.0372
0.0148
1.23
6.42
19.5
42.6
6.58
36.1
6.67

29.4


32
74.1
0.0422
0.0482
0.0129
1.13
5.92
18.9
44.2
7.08
32.8
8.25

26.7


33
60.7
0.0415
0.0442
0.0182
0.48
6.25
16.2
44.6
6.83
29.7
8.17
0.115
29.4


34
67.6
0.132
0.232
0.0116
0.653
5.42
14.2
42.4
6
27.2
10.9
0.224
51.1


35
59.3
0.0608
0.116
0.0186
0.95
5.83
14.3
43.2
5.83
29.8
8.42
0.168
37


36
61.6
0.0443
0.123
0.0164
0.736
6.25
15.8
40.3
6.92
34
8.42

39.9


37
69.3
0.185
0.343
5.83
5.25
40.8
5.58
31.8
9.67



41.8





Table 26: Provided are the values of each of the parameters (as described above) measured in Sorghum accessions (Seed ID) under low nitrogen conditions. Growth conditions are specified in the experimental procedure section.













TABLE 27







Additional measured parameters in Sorghum accessions


under low nitrogen growth conditions


















Seed Id
41
42
43
44
45
46
47
48
49
50
51





















20
96.2
56.3
23.2
5.26
0.105
0.383
215
205
38.3
133
0.505


21
215
79.2
25.6
10.4
0.111
0.402
205
200
39
153
0.506


22
98.6
53.2
20.9
5.93
0.136
0.445
73.5
341
42.3
56.7
0.166


24
183
76.2
28.4
8.25
0.121
0.417
123
241
40.9
195
0.391


25
120
67.3
24.3
6.19
0.141
0.474
153
538
43.1
46.9
0.21


26
110
59.5
22.6
6.12
0.134
0.475
93.2
359
39.9
63.9
0.192


27
172
79.3
32.1
6.8
0.119
0.411
134
149
42.7
342
0.476


28
84.8
51.5
20.4
5.25
0.117
0.405
77.4
129
43.3
215
0.375


29
156
69.9
26.7
7.52
0.116
0.409
130
179
39
286
0.42


30
137
66.2
26.3
6.59
0.129
0.428
99.8
124
42.7
295
0.441


31
138
67.4
25.4
6.85
0.131
0.446
76.9
101
40.1
288
0.429


32
96.5
57.9
23.1
5.32
0.12
0.42
84.2
132
44
202
0.387


33
158
70.6
27.9
7.25
0.116
0.407
92.2
118
45.4
247
0.438


34
164
73.8
28.9
7.19
0.115
0.411
139
177
44.8
289
0.439


35
138
66.9
27.6
6.27
0.107
0.4
113
144
42.6
254
0.442


36
135
65.4
25.5
6.57
0.121
0.414
95.5
127
43.8
316
0.43


37
166
76
30.3
6.82
0.109
0.395
129
180
46.7
232
0.417





Table 27: Provided are the values of each of the parameters (as described above) measured in Sorghum accessions (Seed ID) under low nitrogen conditions. Growth conditions are specified in the experimental procedure section.













TABLE 28







Measured parameters in Sorghum accessions under drought conditions



















Seed Id
52
53
54
55
56
57
58
59
60
61
62
63






















20
0.215
0.205
0.0259
2.36
0.653
0.251
5.33
10.8
38.3
4.5
22.2
7.75


21
0.205
0.2
0.0306
2.44
0.823
0.255
5.5
8
39
5.75
18.5
7.92


22
0.0735
0.341
0.0194
3.67
1.58
0.181
5.83
16
42.3
5.75
30.3
8.67


24
0.123
0.241
0.0356
2.44
1.65
0.365
5.25
10.7
40.9
5.25
22.6
8.25


25
0.153
0.538
0.0252
2.7
0.959
0.189
5.25
13.8
43.1
5.5
27.9
8.17


26
0.0932
0.359
0.0222
3.03
1.32
0.182
6.25
16.3
39.9
6
32.7
9.38


27
0.134
0.149
0.05
2.42
3.47
0.358
6.5
15.2
42.7
6.17
32.2
9.12


28
0.0774
0.129
0.0275
2.19
2.76
0.175
5.75
13.5
43.3
5.83
28.9
8.58


29
0.13
0.179
0.0511
2.57
4.03
0.384
6.5
16.4
39
5.17
26.8
9.5


30
0.0998
0.124
0.0368
2.26
3.03
0.265
6.25
17.6
42.7
5.92
34.2
7.83


31
0.0769
0.101
0.0294
2.19
3.13
0.215
6.75
15.8
40.1
6.08
30.5
7.25


32
0.0842
0.132
0.0267
2.16
3.13
0.182
6.25
17
44
5.92
30.6
9


33
0.0922
0.118
0.0294
2.42
2.39
0.343
6.42
13.9
45.4
6.08
27.2
7.5


34
0.139
0.177
0.0511
2.71
3.34
0.479
6.33
14.8
44.8
6.08
27.6
10


35
0.113
0.144
0.037
2.29
2.7
0.246
5.5
12.5
42.6
5.42
28
8.38


36
0.0955
0.127
0.0399
2.08
4.19
0.219
6.33
17.6
43.8
5.33
29.3
8.67


37
0.129
0.18
0.0418
2.52
2.98
0.26
5.58
15.9
46.7
5.5
30.4
10





Table 28: Provided are the values of each of the parameters (as described above) measured in Sorghum accessions (Seed ID) under drought conditions. Growth conditions are specified in the experimental procedure section.













TABLE 29







Additional measured parameters in Sorghum accessions under drought growth conditions





















Seed Id
64
65
66
67
68
69
70
71
72
73
74
75
76
77
























20
38
7.08
50.2
7.75
38


22.1
83.1
52.8
21.6
4.83
132
0.10


21
30.8
8.58
45.2
7.92
30.8
0.10
0.385
16.8
108
64.5
21.9
6.31
128
0.18


22
111
9.08
92.1
8.67
60.8


9.19
88.7
56.6
21.6
5.16
257
0.16


24
42.8
9
67.2
8.25
42.8
0.12
0.411
104
136
64.4
22
7.78
257
0.22


25
49.6
9.58
73.9
8.17
49.6








0.17


26
49.8
9.75
100
9.38
49.8
0.11
0.41
3.24
90.8
53.2
21
5.28
8.76
0.21


27
46.9
9.08
58.7
9.12
46.9


22
124
71.7
28.6
5.49
248
0.15


28
41.9
7.75
70.8
8.58
41.9
0.10
0.373
9.97
86.1
55.6
21.3
5.04
197
0.08


29
46.1
8.5
67.7
8.25
46.1
0.09
0.364
18.6
85.2
53
20.8
5.07
213
0.14


30
50.2
7.08
68.5
7.83
50.2


29.3
113
69.8
24.7
5.77
325


31
43.6
6.75
65.7
13.1
43.6


10.5
101
65.1
24.3
5.37
282
0.11


32
50.8
8.25
79.2
9
50.9


14.8
80.4
55.3
21.9
4.66
300
0.12


33
42.4
7.92
57.7
7.5
42.4


12.9
127
69.1
25
6.35
292
0.11


34
45.5
9.83
78.6
10
45.5


18.2
86.4
53.3
19.5
5.58
90.2
0.27


35
50.4
8.5
60.8
8.38
50.4


11.6
92.3
56.3
20.4
5.76
105
0.13


36
48.8
9.17
71.2
8.67
53.8


18.6
77.9
49.1
16.8
5.86
148
0.12


37
49.8
10.6
73.9
9.5
51.4
0.12
0.406
16.4
76.9
51.9
18.9
5.1
84.6





Table 29: Provided are the values of each of the parameters (as described above) measured in Sorghum accessions (Seed ID) under drought conditions. Growth conditions are specified in the experimental procedure section.













TABLE 30







Correlation between the expression level of selected LNU genes of some embodiments of the


invention in various tissues and the phenotypic performance under low nitrogen, normal or


drought stress conditions across Sorghum accessions
















Gene

P
Exp.
Corr.
Gene

P
Exp.
Corr.


Name
R
value
Set ID
set
Name
R
value
Set ID
set



















LNU280
0.75
1.91E−02
B
6
LNU278
0.75
1.19E−02
G
67


LNU85
0.71
3.17E−02
B
6
LNU202
0.73
2.45E−02
B
26


LNU279
0.70
3.53E−02
A
6
LNU202
0.71
3.09E−02
B
26


LNU32
0.85
3.53E−03
A
6
LNU84
0.79
6.65E−03
I
58


LNU280
0.70
3.52E−02
B
7
LNU84
0.76
1.11E−02
F
33


LNU279
0.76
1.73E−02
A
7
LNU87
0.71
2.11E−02
F
33


LNU32
0.86
2.96E−03
A
7
LNU25
0.74
1.38E−02
D
33


LNU278
0.73
1.63E−02
H
53
LNU87
0.72
1.97E−02
B
1


LNU87
0.71
2.11E−02
C
15
LNU87
0.71
2.17E−02
B
1


LNU280
0.89
1.22E−03
B
9
LNU202
0.71
3.11E−02
B
24


LNU279
0.76
1.74E−02
C
8
LNU202
0.71
3.11E−02
B
27


LNU280
0.73
1.59E−02
D
47
LNU84
0.89
3.10E−03
G
77


LNU87
0.83
2.92E−03
D
47
LNU84
0.80
1.82E−02
G
77


LNU84
0.70
2.31E−02
D
29
LNU280
0.87
4.92E−03
G
77


LNU279
0.74
1.53E−02
G
72
LNU279
0.82
4.60E−02
E
39


LNU84
0.80
5.67E−03
I
74
LNU84
0.72
1.82E−02
I
56


LNU279
0.73
1.73E−02
G
74
LNU202
0.71
2.07E−02
H
54


LNU279
0.70
2.29E−02
F
74
LNU278
0.95
7.45E−05
F
31


LNU84
0.72
1.86E−02
I
73
LNU280
0.76
1.86E−02
D
31


LNU84
0.70
2.36E−02
I
75
LNU279
0.71
2.23E−02
B
17


LNU280
0.77
9.16E−03
H
75
LNU280
0.77
9.13E−03
A
17


LNU278
0.78
8.14E−03
B
11
LNU87
0.72
1.97E−02
B
20


LNU278
0.76
1.04E−02
B
11
LNU25
0.75
1.26E−02
I
57


LNU84
0.95
2.28E−05
I
61
LNU279
0.75
1.94E−02
B
3


LNU278
0.71
2.26E−02
E
36
LNU202
0.76
1.82E−02
B
3


LNU280
0.71
2.11E−02
G
63
LNU280
0.88
1.84E−03
A
3


LNU202
0.73
2.45E−02
B
23
LNU279
0.86
1.29E−03
F
40


LNU280
0.76
1.15E−02
G
65
LNU84
0.75
1.27E−02
D
40


LNU202
0.87
1.02E−03
I
67
LNU280
0.76
1.78E−02
C
3


LNU278
0.89
5.06E−04
I
67
LNU202
0.76
1.14E−02
H
57







LNU280
0.78
8.43E−03
C
17





Table 30. “Correl. Set ID”—correlation set ID according to the correlated parameters Table above.













TABLE 31







Correlation between the expression level of selected LNU homologous genes of some embodiments of the


invention in various tissues and the phenotypic performance under low nitrogen, normal or drought stress


conditions across Sorghum accessions




















Correl.




Correl.




P
Exp.
Set


P
Exp.
Set


Gene Name
R
value
Set
ID
Gene Name
R
value
Set
ID



















LNU267_H2
0.82
7.37E−03
C
6
LNU265_H0
0.81
4.77E−03
G
63


LNU267_H2
0.82
7.37E−03
C
6
LNU265_H0
0.72
1.84E−02
G
63


LNU74_H173
0.85
3.59E−03
C
6
LNU265_H0
0.81
4.77E−03
G
63


LNU74_H173
0.83
5.54E−03
C
6
LNU265_H0
0.72
1.84E−02
G
63


LNU74_H173
0.85
3.59E−03
C
6
LNU153_H6
0.80
5.43E−03
G
63


LNU74_H173
0.83
5.54E−03
C
6
LNU153_H6
0.80
5.43E−03
G
63


LNU46_H58
0.86
2.71E−03
C
6
LNU271_H4
0.90
8.49E−04
C
23


LNU46_H58
0.73
2.66E−02
C
6
LNU271_H4
0.90
9.96E−04
C
23


LNU46_H58
0.72
2.74E−02
C
6
LNU271_H4
0.90
8.49E−04
C
23


LNU46_H58
0.86
2.71E−03
C
6
LNU271_H4
0.90
9.96E−04
C
23


LNU46_H58
0.73
2.66E−02
C
6
LNU45_H259
0.71
3.28E−02
C
23


LNU46_H58
0.72
2.74E−02
C
6
LNU71_H3
0.81
4.39E−03
I
65


LNU2_H4
0.77
1.61E−02
B
6
LNU71_H3
0.81
4.39E−03
I
65


LNU2_H4???
0.77
1.61E−02
B
6
LNU28_H4
0.77
9.28E−03
H
65


LNU2_H4
0.77
1.61E−02
B
6
LNU28_H4
0.76
1.12E−02
H
65


LNU73_H2
0.78
1.37E−02
B
6
LNU28_H4
0.77
9.28E−03
H
65


LNU73_H2
0.74
2.30E−02
B
6
LNU28_H4
0.76
1.12E−02
H
65


LNU73_H2
0.78
1.37E−02
B
6
LNU71_H3
0.72
1.99E−02
G
65


LNU73_H2
0.74
2.30E−02
B
6
LNU71_H3
0.72
1.99E−02
G
65


LNU76_H55
0.90
8.36E−04
B
6
LNU192_H3
0.75
1.16E−02
G
65


LNU76_H55
0.79
1.10E−02
B
6
LNU192_H3
0.75
1.16E−02
G
65


LNU76_H55
0.90
8.36E−04
B
6
LNU153_H6
0.73
1.59E−02
G
65


LNU76_H55
0.79
1.10E−02
B
6
LNU153_H6
0.73
1.59E−02
G
65


LNU35_H4
0.72
2.88E−02
B
6
LNU109_H2
0.82
3.46E−03
F
38


LNU35_H4
0.72
2.88E−02
B
6
LNU109_H2
0.73
1.67E−02
F
38


LNU223_H6
0.74
2.25E−02
B
6
LNU109_H2
0.82
3.46E−03
F
38


LNU223_H6
0.74
2.25E−02
B
6
LNU109_H2
0.73
1.67E−02
F
38


LNU267_H2
0.75
2.05E−02
A
6
LNU153_H6
0.73
1.75E−02
F
38


LNU267_H2
0.72
3.00E−02
A
6
LNU153_H6
0.73
1.75E−02
F
38


LNU267_H2
0.75
2.05E−02
A
6
LNU28_H4
0.90
4.11E−04
E
38


LNU267_H2
0.72
3.00E−02
A
6
LNU28_H4
0.89
5.37E−04
E
38


LNU263_H4
0.75
2.03E−02
A
6
LNU28_H4
0.90
4.11E−04
E
38


LNU263_H4
0.75
2.07E−02
A
6
LNU28_H4
0.89
5.37E−04
E
38


LNU263_H5
0.75
1.93E−02
A
6
LNU153_H5
0.79
6.64E−03
B
25


LNU263_H5
0.74
2.14E−02
A
6
LNU153_H5
0.79
6.64E−03
B
25


LNU263_H4
0.75
2.03E−02
A
6
LNU45_H258
0.77
9.31E−03
A
25


LNU263_H4
0.75
2.07E−02
A
6
LNU45_H258
0.77
9.31E−03
A
25


LNU263_H5
0.75
1.93E−02
A
6
LNU263_H4
0.73
1.64E−02
I
67


LNU263_H5
0.74
2.14E−02
A
6
LNU263_H4
0.71
2.20E−02
I
67


LNU32_H2
0.76
1.78E−02
A
6
LNU263_H4
0.73
1.64E−02
I
67


LNU32_H2
0.76
1.78E−02
A
6
LNU263_H4
0.71
2.20E−02
I
67


LNU109_H2
0.88
1.67E−03
A
6
LNU19_H1
0.75
1.26E−02
H
67


LNU109_H2
0.88
1.78E−03
A
6
LNU263_H5
0.71
2.22E−02
H
67


LNU109_H2
0.88
1.67E−03
A
6
LNU263_H5
0.71
2.22E−02
H
67


LNU109_H2
0.88
1.78E−03
A
6
LNU271_H4
0.83
2.65E−03
H
67


LNU45_H259
0.75
1.93E−02
A
6
LNU271_H4
0.72
1.82E−02
H
67


LNU73_H2
0.74
1.46E−02
F
45
LNU271_H4
0.83
2.65E−03
H
67


LNU73_H2
0.74
1.46E−02
F
45
LNU271_H4
0.72
1.82E−02
H
67


LNU267_H2
0.87
2.55E−03
C
7
LNU223_H6
0.78
7.24E−03
H
67


LNU267_H2
0.71
3.24E−02
C
7
LNU223_H6
0.78
7.24E−03
H
67


LNU267_H2
0.87
2.55E−03
C
7
LNU271_H4
0.90
8.49E−04
C
26


LNU267_H2
0.71
3.24E−02
C
7
LNU271_H4
0.90
9.96E−04
C
26


LNU74_H173
0.81
8.49E−03
C
7
LNU271_H4
0.90
8.49E−04
C
26


LNU74_H173
0.77
1.44E−02
C
7
LNU271_H4
0.90
9.96E−04
C
26


LNU74_H173
0.81
8.49E−03
C
7
LNU45_H259
0.71
3.28E−02
C
26


LNU74_H173
0.77
1.44E−02
C
7
LNU266_H0
0.91
2.13E−04
I
58


LNU46_H58
0.85
3.94E−03
C
7
LNU266_H0
0.87
1.17E−03
I
58


LNU46_H58
0.85
3.94E−03
C
7
LNU266_H0
0.91
2.13E−04
I
58


LNU19_H1
0.74
2.38E−02
B
7
LNU266_H0
0.87
1.17E−03
I
58


LNU73_H2
0.74
2.27E−02
B
7
LNU271_H4
0.86
1.38E−03
I
58


LNU73_H2
0.74
2.27E−02
B
7
LNU271_H4
0.76
1.14E−02
I
58


LNU76_H55
0.90
8.98E−04
B
7
LNU271_H4
0.86
1.38E−03
I
58


LNU76_H55
0.76
1.78E−02
B
7
LNU271_H4
0.76
1.14E−02
I
58


LNU76_H55
0.90
8.98E−04
B
7
LNU266_H0
0.81
4.11E−03
H
58


LNU76_H55
0.76
1.78E−02
B
7
LNU266_H0
0.75
1.16E−02
H
58


LNU223_H6
0.76
1.80E−02
B
7
LNU266_H0
0.81
4.11E−03
H
58


LNU223_H6
0.76
1.80E−02
B
7
LNU266_H0
0.75
1.16E−02
H
58


LNU267_H2
0.81
7.62E−03
A
7
LNU268_H2
0.81
4.08E−03
H
58


LNU267_H2
0.79
1.15E−02
A
7
LNU268_H2
0.80
4.97E−03
H
58


LNU267_H2
0.81
7.62E−03
A
7
LNU268_H2
0.81
4.08E−03
H
58


LNU267_H2
0.79
1.15E−02
A
7
LNU268_H2
0.80
4.97E−03
H
58


LNU263_H4
0.76
1.81E−02
A
7
LNU121_H1
0.74
1.37E−02
E
33


LNU263_H4
0.75
1.98E−02
A
7
LNU121_H1
0.71
2.17E−02
E
33


LNU263_H4
0.76
1.81E−02
A
7
LNU268_H2
0.92
1.97E−04
E
33


LNU263_H4
0.75
1.98E−02
A
7
LNU268_H2
0.90
3.81E−04
E
33


LNU32_H2
0.73
2.63E−02
A
7
LNU268_H2
0.92
1.97E−04
E
33


LNU32_H2
0.73
2.63E−02
A
7
LNU268_H2
0.90
3.81E−04
E
33


LNU109_H2
0.88
1.95E−03
A
7
LNU48_H1
0.85
1.63E−03
E
33


LNU109_H2
0.86
2.61E−03
A
7
LNU48_H1
0.85
1.63E−03
E
33


LNU109_H2
0.88
1.95E−03
A
7
LNU34_H1
0.78
8.40E−03
E
33


LNU109_H2
0.86
2.61E−03
A
7
LNU34_H1
0.78
8.40E−03
E
33


LNU45_H259
0.75
2.09E−02
A
7
LNU28_H4
0.71
2.27E−02
D
33


LNU45_H259
0.78
8.02E−03
I
53
LNU28_H4
0.71
2.27E−02
D
33


LNU45_H259
0.78
8.02E−03
I
53
LNU45_H259
0.86
1.53E−03
D
33


LNU69_H4
0.70
2.34E−02
H
53
LNU45_H259
0.85
1.76E−03
D
33


LNU69_H4
0.72
1.97E−02
G
53
LNU267_H2
0.73
1.73E−02
A
18


LNU2_H4
0.71
2.09E−02
B
15
LNU267_H2
0.71
2.20E−02
A
18


LNU2_H???
0.71
2.09E−02
B
15
LNU267_H2
0.73
1.73E−02
A
18


LNU2_H4
0.71
2.09E−02
B
15
LNU267_H2
0.71
2.20E−02
A
18


LNU267_H2
0.77
9.13E−03
B
15
LNU73_H2
0.73
1.55E−02
A
18


LNU267_H2
0.77
9.13E−03
B
15
LNU73_H2
0.71
2.03E−02
A
18


LNU153_H5
0.83
3.20E−03
B
15
LNU73_H2
0.73
1.55E−02
A
18


LNU153_H5
0.83
3.20E−03
B
15
LNU73_H2
0.71
2.03E−02
A
18


LNU45_H259
0.75
1.20E−02
I
53
LNU76_H55
0.76
1.06E−02
A
18


LNU45_H259
0.75
1.20E−02
I
53
LNU76_H55
0.75
1.17E−02
A
18


LNU28_H4
0.76
1.02E−02
G
53
LNU76_H55
0.76
1.06E−02
A
18


LNU28_H4
0.76
1.02E−02
G
53
LNU76_H55
0.75
1.17E−02
A
18


LNU153_H5
0.78
7.18E−03
G
53
LNU52_H6
0.84
2.20E−03
B
1


LNU153_H5
0.78
7.18E−03
G
53
LNU52_H6
0.74
1.36E−02
B
1


LNU239_H10
0.72
1.81E−02
E
30
LNU52_H6
0.84
2.20E−03
B
1


LNU239_H10
0.72
1.81E−02
E
30
LNU52_H6
0.74
1.36E−02
B
1


LNU121_H1
0.73
1.72E−02
D
30
LNU46_H60
0.72
1.85E−02
B
1


LNU121_H1
0.71
2.19E−02
D
30
LNU46_H60
0.72
1.85E−02
B
1


LNU45_H259
0.77
9.46E−03
C
15
LNU35_H4
0.80
5.52E−03
H
59


LNU45_H259
0.74
1.43E−02
C
15
LNU35_H4
0.77
8.61E−03
H
59


LNU76_H55
0.80
5.70E−03
H
30
LNU35_H4
0.80
5.52E−03
H
59


LNU76_H55
0.80
5.70E−03
H
30
LNU35_H4
0.77
8.61E−03
H
59


LNU28_H4
0.80
4.98E−03
G
30
LNU73_H2
0.78
8.29E−03
F
34


LNU28_H4
0.72
1.96E−02
G
30
LNU73_H2
0.78
8.29E−03
F
34


LNU28_H4
0.80
4.98E−03
G
30
LNU73_H2
0.76
1.00E−02
A
19


LNU28_H4
0.72
1.96E−02
G
30
LNU73_H2
0.72
1.95E−02
I
62


LNU153_H5
0.76
1.15E−02
G
30
LNU271_H4
0.73
1.58E−02
I
62


LNU153_H5
0.76
1.15E−02
G
30
LNU271_H4
0.73
1.58E−02
I
62


LNU52_H6
0.71
2.05E−02
F
30
LNU35_H4
0.75
1.25E−02
H
62


LNU52_H6
0.71
2.27E−02
F
30
LNU35_H4
0.74
1.39E−02
H
62


LNU52_H6
0.71
2.05E−02
F
30
LNU35_H4
0.75
1.25E−02
H
62


LNU52_H6
0.71
2.27E−02
F
30
LNU35_H4
0.74
1.39E−02
H
62


LNU7_H125
0.71
2.09E−02
F
30
LNU153_H5
0.77
9.81E−03
H
62


LNU7_H125
0.71
2.09E−02
F
30
LNU153_H5
0.77
9.81E−03
H
62


LNU7_H124
0.71
2.09E−02
F
30
LNU239_H10
0.75
1.29E−02
A
22


LNU7_H125
0.71
2.09E−02
F
30
LNU239_H10
0.75
1.29E−02
A
22


LNU7_H124
0.71
2.09E−02
F
30
LNU76_H55
0.76
1.10E−02
H
64


LNU7_H125
0.71
2.09E−02
F
30
LNU76_H55
0.76
1.10E−02
H
64


LNU45_H260
0.79
6.14E−03
F
30
LNU48_H1
0.76
1.04E−02
G
64


LNU239_H10
0.74
1.47E−02
F
30
LNU48_H1
0.76
1.04E−02
G
64


LNU239_H10
0.74
1.47E−02
F
30
LNU28_H4
0.89
6.25E−04
G
64


LNU89_H5
0.72
1.99E−02
E
30
LNU28_H4
0.79
7.02E−03
G
64


LNU89_H5
0.72
1.99E−02
E
30
LNU28_H4
0.89
6.25E−04
G
64


LNU266_H0
0.79
6.53E−03
D
30
LNU28_H4
0.79
7.02E−03
G
64


LNU266_H0
0.75
1.33E−02
D
30
LNU153_H5
0.83
2.98E−03
G
64


LNU266_H0
0.79
6.53E−03
D
30
LNU153_H5
0.83
2.98E−03
G
64


LNU266_H0
0.75
1.33E−02
D
30
LNU271_H4
0.82
7.37E−03
C
24


LNU46_H58
0.74
1.40E−02
D
30
LNU271_H4
0.80
9.35E−03
C
24


LNU46_H58
0.74
1.40E−02
D
30
LNU271_H4
0.82
7.37E−03
C
24


LNU2_H4
0.72
1.77E−02
B
15
LNU271_H4
0.80
9.35E−03
C
24


LNU2_H???
0.72
1.77E−02
B
15
LNU28_H4
0.71
3.17E−02
B
24


LNU2_H4
0.72
1.77E−02
B
15
LNU28_H4
0.70
3.44E−02
B
24


LNU73_H2
0.71
2.04E−02
B
15
LNU28_H4
0.71
3.17E−02
B
24


LNU73_H2
0.71
2.04E−02
B
15
LNU28_H4
0.70
3.44E−02
B
24


LNU71_H3
0.89
4.70E−04
I
55
LNU268_H2
0.77
8.80E−03
A
24


LNU71_H3
0.75
1.32E−02
I
55
LNU268_H2
0.77
8.80E−03
A
24


LNU71_H3
0.89
4.70E−04
I
55
LNU109_H2
0.81
4.76E−03
E
28


LNU71_H3
0.75
1.32E−02
I
55
LNU109_H2
0.81
4.76E−03
E
28


LNU192_H3
0.78
8.36E−03
I
55
LNU45_H259
0.76
1.10E−02
E
28


LNU192_H3
0.77
9.80E−03
I
55
LNU45_H259
0.73
1.62E−02
E
28


LNU192_H3
0.78
8.36E−03
I
55
LNU45_H260
0.74
1.45E−02
E
28


LNU192_H3
0.77
9.80E−03
I
55
LNU45_H260
0.72
1.98E−02
E
28


LNU74_H172
0.71
2.23E−02
G
55
LNU45_H259
0.76
1.10E−02
E
28


LNU74_H172
0.71
2.23E−02
G
55
LNU45_H259
0.73
1.62E−02
E
28


LNU192_H3
0.76
1.03E−02
G
55
LNU48_H1
0.75
1.16E−02
H
68


LNU192_H3
0.76
1.03E−02
G
55
LNU48_H1
0.75
1.16E−02
H
68


LNU128_H12
0.73
1.77E−02
A
16
LNU35_H4
0.76
1.10E−02
H
68


LNU128_H12
0.73
1.77E−02
A
16
LNU35_H4
0.76
1.10E−02
H
68


LNU268_H2
0.76
1.02E−02
A
16
LNU271_H4
0.82
7.37E−03
C
27


LNU268_H2
0.70
2.37E−02
A
16
LNU271_H4
0.80
9.35E−03
C
27


LNU268_H2
0.76
1.02E−02
A
16
LNU271_H4
0.82
7.37E−03
C
27


LNU268_H2
0.70
2.37E−02
A
16
LNU271_H4
0.80
9.35E−03
C
27


LNU35_H4
0.73
1.76E−02
A
16
LNU28_H4
0.71
3.17E−02
B
27


LNU35_H4
0.73
1.76E−02
A
16
LNU28_H4
0.70
3.44E−02
B
27


LNU153_H6
0.70
2.36E−02
A
16
LNU28_H4
0.71
3.17E−02
B
27


LNU153_H6
0.70
2.36E−02
A
16
LNU28_H4
0.70
3.44E−02
B
27


LNU73_H2
0.71
2.14E−02
B
10
LNU268_H2
0.77
8.80E−03
A
27


LNU73_H2
0.71
2.14E−02
B
10
LNU268_H2
0.77
8.80E−03
A
27


LNU153_H5
0.81
4.91E−03
B
10
LNU28_H4
0.90
2.14E−03
H
77


LNU153_H5
0.81
4.91E−03
B
10
LNU28_H4
0.90
2.19E−03
H
77


LNU223_H6
0.78
7.44E−03
G
52
LNU28_H4
0.90
2.14E−03
H
77


LNU223_H6
0.78
7.44E−03
G
52
LNU28_H4
0.90
2.19E−03
H
77


LNU52_H6
0.73
1.60E−02
B
14
LNU153_H6
0.74
3.50E−02
H
77


LNU52_H6
0.73
1.60E−02
B
14
LNU153_H6
0.74
3.50E−02
H
77


LNU46_H60
0.75
1.93E−02
C
9
LNU121_H1
0.72
4.30E−02
G
77


LNU46_H60
0.75
1.93E−02
C
9
LNU121_H1
0.72
4.30E−02
G
77


LNU267_H2
0.89
1.45E−03
B
9
LNU13_H1
0.86
5.88E−03
G
77


LNU267_H2
0.76
1.75E−02
B
9
LNU13_H1
0.85
7.91E−03
G
77


LNU267_H2
0.89
1.45E−03
B
9
LNU13_H1
0.86
5.88E−03
G
77


LNU267_H2
0.76
1.75E−02
B
9
LNU13_H1
0.85
7.91E−03
G
77


LNU73_H2
0.78
1.40E−02
B
9
LNU268_H2
0.88
3.80E−03
G
77


LNU73_H2
0.70
3.39E−02
B
9
LNU268_H2
0.81
1.59E−02
G
77


LNU73_H2
0.78
1.40E−02
B
9
LNU268_H2
0.88
3.80E−03
G
77


LNU73_H2
0.70
3.39E−02
B
9
LNU268_H2
0.81
1.59E−02
G
77


LNU153_H5
0.73
2.46E−02
B
9
LNU7_H125
0.87
5.38E−03
G
77


LNU153_H5
0.73
2.46E−02
B
9
LNU7_H125
0.87
5.38E−03
G
77


LNU109_H2
0.73
2.64E−02
A
9
LNU7_H124
0.87
5.38E−03
G
77


LNU109_H2
0.72
2.89E−02
A
9
LNU71_H3
0.89
2.92E−03
G
77


LNU109_H2
0.73
2.64E−02
A
9
LNU71_H3
0.88
3.50E−03
G
77


LNU109_H2
0.72
2.89E−02
A
9
LNU71_H3
0.89
2.92E−03
G
77


LNU35_H4
0.74
1.46E−02
F
48
LNU71_H3
0.88
3.50E−03
G
77


LNU35_H4
0.74
1.46E−02
F
48
LNU74_H172
0.87
5.17E−03
G
77


LNU74_H172
0.73
2.61E−02
B
14
LNU74_H172
0.75
3.05E−02
G
77


LNU74_H172
0.73
2.61E−02
B
14
LNU74_H172
0.87
5.17E−03
G
77


LNU45_H258
0.72
2.92E−02
B
14
LNU74_H172
0.75
3.05E−02
G
77


LNU45_H258
0.72
2.92E−02
B
14
LNU192_H3
0.90
2.49E−03
G
77


LNU153_H6
0.72
2.81E−02
A
14
LNU192_H3
0.86
6.31E−03
G
77


LNU153_H6
0.72
2.81E−02
A
14
LNU192_H3
0.90
2.49E−03
G
77


LNU266_H0
0.70
2.31E−02
F
48
LNU192_H3
0.86
6.31E−03
G
77


LNU266_H0
0.70
2.31E−02
F
48
LNU7_H125
0.87
5.38E−03
G
77


LNU46_H58
0.70
2.41E−02
D
48
LNU7_H124
0.87
5.38E−03
G
77


LNU46_H58
0.70
2.41E−02
D
48
LNU7_H125
0.87
5.38E−03
G
77


LNU216_H3
0.71
3.12E−02
C
8
LNU265_H0
0.74
3.66E−02
G
77


LNU216_H3
0.71
3.12E−02
C
8
LNU265_H0
0.74
3.66E−02
G
77


LNU263_H4
0.72
3.01E−02
C
8
LNU45_H259
0.85
7.87E−03
G
77


LNU263_H5
0.79
1.06E−02
C
8
LNU45_H259
0.81
1.42E−02
G
77


LNU263_H5
0.78
1.32E−02
C
8
LNU45_H260
0.73
3.79E−02
G
77


LNU263_H4
0.72
3.01E−02
C
8
LNU45_H258
0.89
2.90E−03
G
77


LNU263_H5
0.79
1.06E−02
C
8
LNU45_H258
0.85
8.01E−03
G
77


LNU263_H5
0.78
1.32E−02
C
8
LNU45_H259
0.85
7.87E−03
G
77


LNU216_H4
0.80
9.11E−03
B
8
LNU45_H259
0.81
1.42E−02
G
77


LNU216_H3
0.81
8.55E−03
B
8
LNU45_H258
0.89
2.90E−03
G
77


LNU216_H3
0.77
1.44E−02
B
8
LNU45_H258
0.85
8.01E−03
G
77


LNU216_H4
0.80
9.11E−03
B
8
LNU35_H4
0.74
3.67E−02
G
77


LNU216_H3
0.81
8.55E−03
B
8
LNU35_H4
0.74
3.67E−02
G
77


LNU216_H3
0.77
1.44E−02
B
8
LNU153_H6
0.89
2.89E−03
G
77


LNU74_H172
0.81
7.93E−03
B
8
LNU153_H6
0.89
2.89E−03
G
77


LNU74_H172
0.81
7.93E−03
B
8
LNU74_H173
0.75
3.19E−02
A
2


LNU45_H259
0.79
1.20E−02
B
8
LNU74_H173
0.75
3.19E−02
A
2


LNU45_H259
0.79
1.20E−02
B
8
LNU46_H60
0.82
4.40E−02
E
39


LNU153_H6
0.80
1.00E−02
B
8
LNU46_H58
0.82
4.70E−02
E
39


LNU153_H6
0.80
1.00E−02
B
8
LNU46_H60
0.82
4.40E−02
E
39


LNU128_H13
0.71
3.37E−02
A
8
LNU46_H58
0.82
4.70E−02
E
39


LNU128_H12
0.80
1.04E−02
A
8
LNU7_H125
0.85
3.33E−02
D
39


LNU128_H13
0.71
3.37E−02
A
8
LNU7_H125
0.85
3.33E−02
D
39


LNU128_H12
0.80
1.04E−02
A
8
LNU7_H124
0.85
3.33E−02
D
39


LNU51_H2
0.71
3.07E−02
A
8
LNU7_H125
0.85
3.33E−02
D
39


LNU51_H2
0.71
3.07E−02
A
8
LNU7_H124
0.85
3.33E−02
D
39


LNU67_H4
0.80
1.03E−02
A
8
LNU7_H125
0.85
3.33E−02
D
39


LNU265_H0
0.82
7.21E−03
A
8
LNU271_H4
0.82
4.76E−02
D
39


LNU265_H0
0.82
7.21E−03
A
8
LNU271_H4
0.82
4.76E−02
D
39


LNU128_H13
0.78
7.57E−03
E
47
LNU153_H5
0.74
1.40E−02
B
13


LNU128_H12
0.78
7.39E−03
E
47
LNU153_H5
0.74
1.40E−02
B
13


LNU128_H12
0.71
2.13E−02
E
47
LNU266_H0
0.71
2.15E−02
G
56


LNU128_H13
0.78
7.57E−03
E
47
LNU266_H0
0.70
2.28E−02
G
56


LNU128_H12
0.78
7.39E−03
E
47
LNU266_H0
0.71
2.15E−02
G
56


LNU128_H12
0.71
2.13E−02
E
47
LNU266_H0
0.70
2.28E−02
G
56


LNU52_H6
0.71
2.20E−02
E
47
LNU223_H6
0.84
2.57E−03
G
56


LNU52_H6
0.71
2.20E−02
E
47
LNU223_H6
0.70
2.34E−02
G
56


LNU263_H4
0.70
2.40E−02
E
47
LNU223_H6
0.84
2.57E−03
G
56


LNU263_H4
0.70
2.40E−02
E
47
LNU223_H6
0.70
2.34E−02
G
56


LNU52_H6
0.79
7.10E−03
B
12
LNU73_H2
0.73
2.55E−02
F
32


LNU52_H6
0.79
7.10E−03
B
12
LNU73_H2
0.73
2.55E−02
F
32


LNU74_H172
0.76
1.08E−02
B
12
LNU45_H259
0.88
1.98E−03
F
32


LNU74_H172
0.74
1.40E−02
B
12
LNU45_H259
0.88
1.98E−03
F
32


LNU74_H172
0.76
1.08E−02
B
12
LNU17_H2
0.77
1.44E−02
E
32


LNU74_H172
0.74
1.40E−02
B
12
LNU17_H2
0.70
3.40E−02
E
32


LNU35_H4
0.81
4.84E−03
E
29
LNU17_H2
0.77
1.44E−02
E
32


LNU35_H4
0.81
4.84E−03
E
29
LNU17_H2
0.70
3.40E−02
E
32


LNU266_H0
0.72
1.84E−02
D
29
LNU109_H2
0.76
1.68E−02
E
32


LNU266_H0
0.70
2.29E−02
D
29
LNU109_H2
0.76
1.68E−02
E
32


LNU266_H0
0.72
1.84E−02
D
29
LNU266_H0
0.77
9.34E−03
C
17


LNU266_H0
0.70
2.29E−02
D
29
LNU266_H0
0.73
1.70E−02
C
17


LNU216_H3
0.71
2.23E−02
G
52
LNU266_H0
0.77
9.34E−03
C
17


LNU216_H3
0.71
2.23E−02
G
52
LNU266_H0
0.73
1.70E−02
C
17


LNU223_H6
0.85
1.66E−03
G
52
LNU216_H4
0.76
1.15E−02
G
54


LNU223_H6
0.71
2.11E−02
G
52
LNU216_H3
0.80
5.03E−03
G
54


LNU223_H6
0.85
1.66E−03
G
52
LNU216_H3
0.79
6.11E−03
G
54


LNU223_H6
0.71
2.11E−02
G
52
LNU216_H4
0.76
1.15E−02
G
54


LNU35_H4
0.76
1.01E−02
E
29
LNU216_H3
0.80
5.03E−03
G
54


LNU35_H4
0.76
1.01E−02
E
29
LNU216_H3
0.79
6.11E−03
G
54


LNU266_H0
0.78
8.03E−03
D
29
LNU74_H172
0.85
1.77E−03
G
54


LNU266_H0
0.76
1.10E−02
D
29
LNU74_H172
0.85
1.77E−03
G
54


LNU266_H0
0.78
8.03E−03
D
29
LNU45_H259
0.78
7.31E−03
G
54


LNU266_H0
0.76
1.10E−02
D
29
LNU45_H260
0.71
2.04E−02
G
54


LNU153_H6
0.73
1.69E−02
A
29
LNU45_H259
0.78
7.31E−03
G
54


LNU153_H6
0.73
1.69E−02
A
29
LNU223_H6
0.87
9.56E−04
G
54


LNU35_H4
0.83
2.65E−03
G
52
LNU223_H6
0.84
2.41E−03
G
54


LNU35_H4
0.83
2.65E−03
G
52
LNU223_H6
0.87
9.56E−04
G
54


LNU265_H0
0.72
1.79E−02
D
29
LNU223_H6
0.84
2.41E−03
G
54


LNU265_H0
0.72
1.79E−02
D
29
LNU128_H12
0.76
1.66E−02
F
31


LNU263_H4
0.73
1.67E−02
C
12
LNU128_H12
0.74
2.35E−02
F
31


LNU263_H5
0.80
5.07E−03
C
12
LNU128_H12
0.76
1.66E−02
F
31


LNU263_H5
0.79
6.53E−03
C
12
LNU128_H12
0.74
2.35E−02
F
31


LNU263_H4
0.73
1.67E−02
C
12
LNU69_H4
0.86
2.75E−03
F
31


LNU263_H5
0.80
5.07E−03
C
12
LNU69_H4
0.74
2.16E−02
D
31


LNU263_H5
0.79
6.53E−03
C
12
LNU153_H6
0.71
2.27E−02
C
17


LNU216_H4
0.81
4.94E−03
B
12
LNU153_H6
0.71
2.27E−02
C
17


LNU216_H3
0.78
7.53E−03
B
12
LNU28_H4
0.77
9.79E−03
B
17


LNU216_H3
0.74
1.39E−02
B
12
LNU28_H4
0.75
1.27E−02
B
17


LNU216_H4
0.81
4.94E−03
B
12
LNU28_H4
0.77
9.79E−03
B
17


LNU216_H3
0.78
7.53E−03
B
12
LNU28_H4
0.75
1.27E−02
B
17


LNU216_H3
0.74
1.39E−02
B
12
LNU153_H6
0.84
2.34E−03
B
17


LNU74_H172
0.82
4.02E−03
B
12
LNU153_H6
0.84
2.34E−03
B
17


LNU74_H172
0.82
4.02E−03
B
12
LNU128_H12
0.72
2.00E−02
A
17


LNU45_H259
0.80
5.93E−03
B
12
LNU128_H12
0.72
2.00E−02
A
17


LNU45_H259
0.80
5.93E−03
B
12
LNU13_H1
0.80
4.97E−03
A
17


LNU153_H6
0.72
1.99E−02
B
12
LNU13_H1
0.78
7.23E−03
A
17


LNU153_H6
0.72
1.99E−02
B
12
LNU13_H1
0.80
4.97E−03
A
17


LNU128_H12
0.76
1.11E−02
A
12
LNU13_H1
0.78
7.23E−03
A
17


LNU128_H12
0.76
1.11E−02
A
12
LNU268_H2
0.87
1.22E−03
A
17


LNU51_H2
0.71
2.07E−02
A
12
LNU268_H2
0.82
3.58E−03
A
17


LNU51_H2
0.71
2.07E−02
A
12
LNU268_H2
0.87
1.22E−03
A
17


LNU67_H4
0.80
5.06E−03
A
12
LNU268_H2
0.82
3.58E−03
A
17


LNU265_H0
0.81
4.14E−03
A
12
LNU52_H6
0.72
1.91E−02
A
17


LNU265_H0
0.81
4.14E−03
A
12
LNU52_H6
0.72
1.91E−02
A
17


LNU121_H1
0.87
1.04E−03
H
72
LNU153_H6
0.89
5.61E−04
A
17


LNU121_H1
0.77
9.39E−03
H
72
LNU153_H6
0.89
5.61E−04
A
17


LNU121_H1
0.87
1.04E−03
H
72
LNU13_H1
0.79
6.40E−03
I
60


LNU121_H1
0.77
9.39E−03
H
72
LNU13_H1
0.79
6.40E−03
I
60


LNU2_H4
0.78
8.24E−03
H
72
LNU71_H3
0.84
2.54E−03
I
60


LNU2_H???
0.78
8.24E−03
H
72
LNU71_H3
0.83
2.96E−03
I
60


LNU2_H4
0.78
8.24E−03
H
72
LNU71_H3
0.84
2.54E−03
I
60


LNU51_H2
0.85
1.96E−03
H
72
LNU71_H3
0.83
2.96E−03
I
60


LNU51_H2
0.85
1.96E−03
H
72
LNU74_H172
0.74
1.44E−02
I
60


LNU32_H2
0.89
5.20E−04
H
72
LNU74_H172
0.74
1.44E−02
I
60


LNU32_H2
0.89
5.20E−04
H
72
LNU192_H3
0.87
1.08E−03
I
60


LNU266_H0
0.73
2.68E−02
A
4
LNU192_H3
0.72
1.77E−02
I
60


LNU266_H0
0.73
2.68E−02
A
4
LNU192_H3
0.87
1.08E−03
I
60


LNU268_H2
0.75
1.98E−02
A
4
LNU192_H3
0.72
1.77E−02
I
60


LNU268_H2
0.72
2.84E−02
A
4
LNU46_H60
0.73
1.71E−02
I
60


LNU268_H2
0.75
1.98E−02
A
4
LNU46_H60
0.73
1.71E−02
I
60


LNU268_H2
0.72
2.84E−02
A
4
LNU73_H2
0.77
8.83E−03
E
35


LNU153_H6
0.77
1.48E−02
A
4
LNU35_H4
0.76
1.00E−02
E
35


LNU153_H6
0.77
1.48E−02
A
4
LNU35_H4
0.76
1.00E−02
E
35


LNU46_H58
0.72
1.93E−02
D
41
LNU52_H6
0.84
2.20E−03
B
20


LNU46_H58
0.72
1.93E−02
D
41
LNU52_H6
0.74
1.36E−02
B
20


LNU268_H2
0.71
2.18E−02
I
74
LNU52_H6
0.84
2.20E−03
B
20


LNU268_H2
0.71
2.18E−02
I
74
LNU52_H6
0.74
1.36E−02
B
20


LNU121_H1
0.83
2.88E−03
H
74
LNU46_H60
0.72
1.85E−02
B
20


LNU121_H1
0.78
8.38E−03
H
74
LNU46_H60
0.72
1.85E−02
B
20


LNU121_H1
0.83
2.88E−03
H
74
LNU109_H2
0.74
1.51E−02
I
71


LNU121_H1
0.78
8.38E−03
H
74
LNU109_H2
0.74
1.51E−02
I
71


LNU51_H2
0.77
9.69E−03
H
74
LNU216_H4
0.74
1.53E−02
G
71


LNU51_H2
0.77
9.69E−03
H
74
LNU216_H3
0.75
1.27E−02
G
71


LNU192_H3
0.77
8.74E−03
H
74
LNU216_H3
0.74
1.41E−02
G
71


LNU192_H3
0.70
2.33E−02
H
74
LNU216_H4
0.74
1.53E−02
G
71


LNU192_H3
0.77
8.74E−03
H
74
LNU216_H3
0.75
1.27E−02
G
71


LNU192_H3
0.70
2.33E−02
H
74
LNU216_H3
0.74
1.41E−02
G
71


LNU32_H2
0.76
1.06E−02
H
74
LNU45_H259
0.75
2.10E−02
C
3


LNU32_H2
0.76
1.06E−02
H
74
LNU153_H6
0.72
2.81E−02
C
3


LNU89_H5
0.71
2.03E−02
H
74
LNU153_H6
0.72
2.81E−02
C
3


LNU89_H5
0.71
2.03E−02
H
74
LNU28_H4
0.77
1.62E−02
B
3


LNU86_H0
0.72
2.01E−02
H
74
LNU28_H4
0.76
1.80E−02
B
3


LNU86_H0
0.72
2.01E−02
H
74
LNU28_H4
0.77
1.62E−02
B
3


LNU46_H58
0.75
1.23E−02
G
74
LNU28_H4
0.76
1.80E−02
B
3


LNU46_H58
0.75
1.23E−02
G
74
LNU153_H6
0.83
5.32E−03
B
3


LNU153_H5
0.71
3.05E−02
B
5
LNU153_H6
0.83
5.32E−03
B
3


LNU153_H5
0.71
3.05E−02
B
5
LNU128_H12
0.72
2.75E−02
A
3


LNU268_H2
0.71
3.17E−02
A
5
LNU128_H12
0.72
2.75E−02
A
3


LNU268_H2
0.71
3.29E−02
A
5
LNU13_H1
0.80
9.65E−03
A
3


LNU268_H2
0.71
3.17E−02
A
5
LNU13_H1
0.78
1.27E−02
A
3


LNU268_H2
0.71
3.29E−02
A
5
LNU13_H1
0.80
9.65E−03
A
3


LNU153_H6
0.80
1.01E−02
A
5
LNU13_H1
0.78
1.27E−02
A
3


LNU153_H6
0.80
1.01E−02
A
5
LNU266_H0
0.76
1.85E−02
A
3


LNU263_H4
0.75
1.20E−02
F
43
LNU266_H0
0.71
3.35E−02
A
3


LNU263_H4
0.75
1.32E−02
F
43
LNU266_H0
0.76
1.85E−02
A
3


LNU263_H4
0.75
1.20E−02
F
43
LNU266_H0
0.71
3.35E−02
A
3


LNU263_H4
0.75
1.32E−02
F
43
LNU268_H2
0.85
3.93E−03
A
3


LNU46_H58
0.72
1.86E−02
D
43
LNU268_H2
0.79
1.06E−02
A
3


LNU46_H58
0.72
1.86E−02
D
43
LNU268_H2
0.85
3.93E−03
A
3


LNU121_H1
0.88
8.89E−04
H
73
LNU268_H2
0.79
1.06E−02
A
3


LNU121_H1
0.78
8.16E−03
H
73
LNU52_H6
0.75
1.94E−02
A
3


LNU121_H1
0.88
8.89E−04
H
73
LNU52_H6
0.75
1.94E−02
A
3


LNU121_H1
0.78
8.16E−03
H
73
LNU67_H4
0.76
1.73E−02
A
3


LNU2_H4
0.72
1.80E−02
H
73
LNU192_H3
0.73
2.47E−02
A
3


LNU2_H???
0.72
1.80E−02
H
73
LNU192_H3
0.73
2.47E−02
A
3


LNU2_H4
0.72
1.80E−02
H
73
LNU265_H0
0.70
3.43E−02
A
3


LNU51_H2
0.84
2.50E−03
H
73
LNU265_H0
0.70
3.43E−02
A
3


LNU51_H2
0.84
2.50E−03
H
73
LNU46_H60
0.73
2.57E−02
A
3


LNU32_H2
0.85
1.87E−03
H
73
LNU46_H60
0.73
2.57E−02
A
3


LNU32_H2
0.85
1.87E−03
H
73
LNU153_H6
0.96
5.15E−05
A
3


LNU265_H0
0.70
2.29E−02
H
73
LNU153_H6
0.96
5.15E−05
A
3


LNU265_H0
0.70
2.41E−02
H
73
LNU216_H4
0.82
3.31E−03
F
40


LNU265_H0
0.70
2.29E−02
H
73
LNU216_H3
0.86
1.42E−03
F
40


LNU265_H0
0.70
2.41E−02
H
73
LNU216_H3
0.86
1.43E−03
F
40


LNU263_H4
0.72
1.77E−02
F
42
LNU216_H4
0.82
3.31E−03
F
40


LNU263_H4
0.71
2.06E−02
F
42
LNU216_H3
0.86
1.42E−03
F
40


LNU263_H4
0.72
1.77E−02
F
42
LNU216_H3
0.86
1.43E−03
F
40


LNU263_H4
0.71
2.06E−02
F
42
LNU263_H4
0.80
5.34E−03
F
40


LNU46_H58
0.75
1.27E−02
D
42
LNU263_H4
0.80
5.88E−03
F
40


LNU46_H58
0.75
1.27E−02
D
42
LNU263_H5
0.71
2.04E−02
F
40


LNU216_H4
0.77
9.16E−03
I
75
LNU263_H4
0.80
5.34E−03
F
40


LNU216_H4
0.77
9.16E−03
I
75
LNU263_H4
0.80
5.88E−03
F
40


LNU46_H60
0.70
2.29E−02
I
75
LNU263_H5
0.71
2.04E−02
F
40


LNU46_H60
0.70
2.29E−02
I
75
LNU271_H4
0.73
1.71E−02
F
40


LNU2_H4
0.75
1.24E−02
H
75
LNU271_H4
0.73
1.71E−02
F
40


LNU2_H???
0.75
1.24E−02
H
75
LNU266_H0
0.70
2.34E−02
E
40


LNU2_H4
0.75
1.24E−02
H
75
LNU266_H0
0.70
2.34E−02
E
40


LNU32_H2
0.73
1.67E−02
H
75
LNU74_H173
0.73
1.63E−02
D
40


LNU32_H2
0.73
1.67E−02
H
75
LNU74_H173
0.73
1.63E−02
D
40


LNU73_H2
0.73
1.56E−02
G
75
LNU45_H258
0.79
6.14E−03
D
40


LNU32_H2
0.75
1.25E−02
G
75
LNU45_H258
0.79
6.14E−03
D
40


LNU32_H2
0.75
1.25E−02
G
75
LNU46_H58
0.80
5.34E−03
D
40


LNU45_H259
0.83
2.77E−03
G
75
LNU46_H58
0.80
5.34E−03
D
40


LNU45_H259
0.78
8.22E−03
G
75
LNU266_H0
0.79
1.14E−02
C
3


LNU266_H0
0.74
1.45E−02
F
44
LNU266_H0
0.77
1.42E−02
C
3


LNU266_H0
0.74
1.45E−02
F
44
LNU266_H0
0.79
1.14E−02
C
3


LNU73_H2
0.70
2.37E−02
G
76
LNU266_H0
0.77
1.42E−02
C
3


LNU74_H173
0.80
5.37E−03
B
11
LNU74_H173
0.79
1.16E−02
B
3


LNU74_H173
0.80
5.37E−03
B
11
LNU74_H173
0.79
1.16E−02
B
3


LNU266_H0
0.72
1.93E−02
A
11
LNU192_H3
0.76
1.74E−02
B
3


LNU266_H0
0.72
1.93E−02
A
11
LNU192_H3
0.76
1.74E−02
B
3


LNU153_H6
0.71
2.21E−02
A
11
LNU266_H0
0.74
2.24E−02
A
3


LNU153_H6
0.71
2.21E−02
A
11
LNU266_H0
0.74
2.24E−02
A
3


LNU266_H0
0.81
4.88E−03
F
50
LNU268_H2
0.77
1.61E−02
A
3


LNU266_H0
0.77
9.63E−03
F
50
LNU268_H2
0.76
1.65E−02
A
3


LNU266_H0
0.81
4.88E−03
F
50
LNU268_H2
0.77
1.61E−02
A
3


LNU266_H0
0.77
9.63E−03
F
50
LNU268_H2
0.76
1.65E−02
A
3


LNU266_H0
0.72
2.00E−02
E
50
LNU153_H6
0.90
9.44E−04
A
3


LNU266_H0
0.72
2.00E−02
E
50
LNU153_H6
0.90
9.44E−04
A
3


LNU266_H0
0.80
5.96E−03
D
50
LNU216_H3
0.70
2.30E−02
F
32


LNU266_H0
0.79
7.09E−03
D
50
LNU216_H3
0.70
2.34E−02
F
32


LNU266_H0
0.80
5.96E−03
D
50
LNU216_H3
0.70
2.30E−02
F
32


LNU266_H0
0.79
7.09E−03
D
50
LNU216_H3
0.70
2.34E−02
F
32


LNU46_H58
0.81
4.21E−03
D
50
LNU266_H0
0.72
1.83E−02
F
32


LNU46_H58
0.81
4.21E−03
D
50
LNU266_H0
0.72
1.83E−02
F
32


LNU266_H0
0.72
1.96E−02
I
61
LNU51_H2
0.74
1.40E−02
I
57


LNU266_H0
0.72
1.96E−02
I
61
LNU51_H2
0.74
1.40E−02
I
57


LNU271_H4
0.87
9.39E−04
I
61
LNU74_H172
0.74
1.54E−02
G
57


LNU271_H4
0.85
1.86E−03
I
61
LNU74_H172
0.70
2.39E−02
G
57


LNU271_H4
0.87
9.39E−04
I
61
LNU74_H172
0.74
1.54E−02
G
57


LNU271_H4
0.85
1.86E−03
I
61
LNU74_H172
0.70
2.39E−02
G
57


LNU45_H259
0.80
4.99E−03
F
36
LNU45_H259
0.76
1.14E−02
G
57


LNU45_H259
0.80
4.99E−03
F
36
LNU45_H259
0.74
1.46E−02
G
57


LNU121_H1
0.77
9.58E−03
E
36
LNU45_H258
0.72
1.97E−02
G
57


LNU121_H1
0.77
9.26E−03
E
36
LNU45_H258
0.71
2.04E−02
G
57


LNU121_H1
0.77
9.58E−03
E
36
LNU45_H259
0.76
1.14E−02
G
57


LNU67_H4
0.73
1.57E−02
E
36
LNU45_H259
0.74
1.46E−02
G
57


LNU86_H0
0.74
1.37E−02
E
36
LNU45_H258
0.72
1.97E−02
G
57


LNU86_H0
0.74
1.37E−02
E
36
LNU45_H258
0.71
2.04E−02
G
57


LNU71_H3
0.71
2.15E−02
I
63
LNU266_H0
0.81
4.59E−03
C
3


LNU71_H3
0.71
2.15E−02
I
63
LNU266_H0
0.79
6.43E−03
C
3


LNU28_H4
0.81
4.19E−03
H
63
LNU266_H0
0.81
4.59E−03
C
3


LNU28_H4
0.81
4.23E−03
H
63
LNU266_H0
0.79
6.43E−03
C
3


LNU28_H4
0.81
4.19E−03
H
63
LNU74_H173
0.70
2.36E−02
B
3


LNU28_H4
0.81
4.23E−03
H
63
LNU74_H173
0.70
2.36E−02
B
3


LNU71_H3
0.81
4.65E−03
G
63
LNU192_H3
0.72
1.98E−02
B
3


LNU71_H3
0.77
9.48E−03
G
63
LNU192_H3
0.72
1.98E−02
B
3


LNU71_H3
0.81
4.65E−03
G
63
LNU268_H2
0.79
6.71E−03
A
3


LNU71_H3
0.77
9.48E−03
G
63
LNU268_H2
0.79
6.95E−03
A
3


LNU192_H3
0.77
9.48E−03
G
63
LNU268_H2
0.79
6.71E−03
A
3


LNU192_H3
0.72
1.81E−02
G
63
LNU268_H2
0.79
6.95E−03
A
3


LNU192_H3
0.77
9.48E−03
G
63
LNU153_H6
0.85
1.63E−03
A
3


LNU192_H3
0.72
1.81E−02
G
63
LNU153_H6
0.85
1.63E−03
A
3





Table 31. “Correl. Set ID”—correlation set ID according to the correlated parameters Table above.






Example 8
Production of Sorghum Transcriptom and High Throughput Correlation Analysis with Yield, NUE, and ABST Related Parameters Measured 1N Semi-Hydroponics Conditions Using 44K Sorguhm Oligonucleotide Micro-Arrays
Sorghum vigor related parameters under low nitrogen, 100 mM NaCl, low temperature (10±2° C.) and normal growth conditions—Ten Sorghum hybrids were grown in 3 repetitive plots, each containing 17 plants, at a net house under semi-hydroponics conditions. Briefly, the growing protocol was as follows: Sorghum seeds were sown in trays filled with a mix of vermiculite and peat in a 1:1 ratio. Following germination, the trays were transferred to the high salinity solution (100 mM NaCl in addition to the Full Hoagland solution), low temperature (10±2° C. in the presence of Full Hoagland solution), low nitrogen solution (the amount of total nitrogen was reduced in 90% from the full Hoagland solution (i.e., to a final concentration of 10% from full Hoagland solution, final amount of 1.2 mM N) or at Normal growth solution (Full Hoagland containing 16 mM N solution, at 28±2° C.). Plants were grown at 28±2° C.
Full Hoagland solution consists of: KNO3-0.808 grams/liter, MgSO4—0.12 grams/liter, KH2PO4—0.172 grams/liter and 0.01% (volume/volume) of ‘Super coratin’ micro elements (Iron-EDDHA [ethylenediamine-N,N′-bis(2-hydroxyphenylacetic acid)]-40.5 grams/liter; Mn—20.2 grams/liter; Zn 10.1 grams/liter; Co 1.5 grams/liter; and Mo 1.1 grams/liter), solution's pH should be 6.5-6.8].
Analyzed Sorghum tissues—All 10 selected Sorghum hybrids were sampled per each treatment. Three tissues [leaves, meristems and roots] growing at 100 mM NaCl, low temperature (10±2° C.), low Nitrogen (1.2 mM N) or under Normal conditions were sampled and RNA was extracted as described above. Each micro-array expression information tissue type has received a Set ID as summarized in Table 32 below.







TABLE 32







Sorghum transcriptom expression sets under semi hydroponics conditions










Expression set
Set Id







Sorghum roots under Low Nitrogen
A



Sorghum leaves under Low Nitrogen
B



Sorghum meristems under Low Nitrogen
C



Sorghum roots under Normal Growth
D



Sorghum leaves under Normal Growth
E



Sorghum meristems under Normal Growth
F



Sorghum roots under 100 mM NaCl
G



Sorghum leaves under 100 mM NaCl
H



Sorghum meristems under 100 mM NaCl
I



Sorghum roots under cold
J



Sorghum leaves under cold
K



Sorghum meristems under cold
L







Table 32: Provided are the Sorghum transcriptom expression sets.



Cold conditions = 10 ± 2° C.;



NaCl = 100 mM NaCl;



low nitrogen = 1.2 mM Nitrogen;



Normal conditions = 16 mM Nitrogen.






Experimental Results
10 different Sorghum hybrids were grown and characterized for the following parameters: “Leaf number Normal”=leaf number per plant under normal conditions (average of five plants); “Plant Height Normal”=plant height under normal conditions (average of five plants); “Root DW”—root dry weight per plant (average of five plants); The average for each of the measured parameter was calculated using the JMP software and values are summarized in Table 34, 35, 36 and 37 below. Subsequent correlation analysis was performed (Tables 38 and 39). Results were then integrated to the database.







TABLE 33







Sorghum correlated parameters (vectors)










Correlation Set
Correlation ID














Plant Height TP1 - Low Nitrogen
1



Plant Height TP2- Low Nitrogen
2



Plant Height TP3 - Low Nitrogen
3



Leaf TP1 - Low Nitrogen
4



Leaf TP2 - Low Nitrogen
5



Leaf TP3 - Low Nitrogen
6



DW Shoot/Plant - Low Nitrogen
7



DW Root/Plant - Low Nitrogen
8



SPAD - Low Nitrogen
9



Plant Height TP1 - 100 mM NaCl
10



Plant Height TP2 - 100 mM NaCl
11



Plant Height TP3 - 100 mM NaCl
12



Leaf TP1 - 100 mM NaCl
13



Leaf TP2 - 100 mM NaCl
14



Leaf TP3 - 100 mM NaCl
15



DW Shoot/Plant - 100 mM NaCl
16



DW Root/Plant - 100 mM NaCl
17



SPAD - 100 mM NaCl
18



Plant Height TP1-Cold
19



Plant Height TP2- Cold
20



Leaf TP1 - Cold
21



Leaf TP2 - Cold
22



Leaf TP3 - Cold
23



DW Shoot/Plant - Cold
24



DW Root/Plant - Cold
25



SPAD - Cold
26



Plant Height TP1-Normal
27



Plant Height TP2-Normal
28



Leaf TP1 -Normal
29



Leaf TP2 -Normal
30



Leaf TP3 -Normal
31



DW Shoot/Plant - Normal
32



DW Root/Plant - Normal
33



SPAD - Normal
34







Table 33: Provided are the Sorghum correlated parameters.



Cold conditions = 10 ± 2° C.;



NaCl = 100 mM NaCl;



low nitrogen = 1.2 mM Nitrogen;



Normal conditions = 16 mM Nitrogen * TP-1-2-3 refers to time points 1, 2 and 3.













TABLE 34







Sorghum accessions, measured parameters under low nitrogen growth conditions
















Seed ID
1
2
3
4
5
6
7
8
9



















20
6.73
13.3
22.2
3
4
3.9
0.0823
0.0444
26.9


22
9.77
20.6
31.1
3.13
4.58
4.27
0.187
0.108
28


26
12.7
23.7
34.7
3.87
4.97
4.7
0.328
0.202
29.6


27
8.67
18
30
3.53
4.73
4.23
0.163
0.104
31.5


28
9.77
19.3
30.8
3.2
4.6
4.3
0.163
0.0777
29.6


29
9.23
19.2
29.9
3.13
4.7
4.57
0.156
0.0858
26.8


30
10.3
21.9
30.9
3.13
4.97
4.63
0.259
0.13
28.5


31
10.1
22.1
32.4
3.3
4.87
4.67
0.199
0.0944
28.2


34
7.93
18.2
29.4
3.07
4.67
3.97
0.13
0.0863
30.5


37
8.23
21
30.7
3.07
4.57
4.1
0.184
0.0924
27.6





Table 34: Provided are the values of each of the parameters (as described above) measured in Sorghum accessions (Seed ID) under low nitrogen conditions. Growth conditions are specified in the experimental procedure section.













TABLE 35







Sorghum accessions, measured parameters under 100 mM NaCl growth conditions
















Seed ID
10
11
12
13
14
15
16
17
18



















20
7.9
14.2
21.8
3
4
4
0.0943
0.05
32.7


22
9.5
16.3
23.2
3.13
4.37
4.13
0.186
0.104
35.1


26
10.9
20.4
30.4
3.4
4.87
4.57
0.202
0.124
28


27
7.93
13.3
22.8
3.07
4.6
4.43
0.137
0.0688
30.9


28
9.7
15.9
23.7
3.33
4.5
4.07
0.13
0.0757
34.5


29
8.53
16.5
23.3
3.07
4.53
4.33
0.133
0.0752
30


30
8.9
15.5
22.5
3.07
4.5
4.13
0.154
0.135
32.1


31
10.4
18.9
26.8
3.27
4.77
4.5
0.189
0.0955
31.9


34
7
13.7
20.3
3
4.32
3.78
0.0993
0.165
32.5


37
7.83
15.8
23.6
3.07
4.2
4.2
0.124
0.139
34.3





Table 35: Provided are the values of each of the parameters (as described above) measured in Sorghum accessions (Seed ID) under 100 mM NaCl growth conditions. Growth conditions are specified in the experimental procedure section.













TABLE 36







Sorghum accessions, measured parameters under cold growth conditions















Seed ID
19
20
21
22
23
24
25
26


















20
6.5
11.2
3
3.9
4.73
0.0781
0.0681
28.6


22
8.77
15.9
3
4.13
5.33
0.154
0.108
30.3


26
10.4
18.4
3.5
4.63
5.43
0.189
0.163
27


27
6.8
12.2
3.17
4.17
5.5
0.112
0.0935
32.3


28
9.03
16
3.4
4.27
5.33
0.13
0.0835
28.3


29
9
14.6
3.2
4.23
5.07
0.165
0.114
29.9


30
7.97
14.6
3.13
4.2
4.5
0.152
0.137
32.5


31
9.17
17.3
3.07
4.3
5.4
0.15
0.127
28.6


34
6.5
13.4
3.07
4.17
5.37
0.112
0.108
31.7


37
7.23
13.9
3
4
5.18
0.141
0.139
29.6





Table 36: Provided are the values of each of the parameters (as described above) measured in Sorghum accessions (Seed ID) under cold growth conditions. Growth conditions are specified in the experimental procedure section.













TABLE 37







Sorghum accessions, measured parameters under regular growth


conditions















Seed ID
27
28
29
30
31
32
33
34


















20
7.47
15
3
4.17
5.33
0.101
0.0525
26.7


22
9.3
18.2
3.07
4.5
5.87
0.236
0.134
29.3


26
12.9
22.1
3.8
4.8
6.2
0.313
0.172
29.9


27
8.57
17.6
3.2
4.6
5.8
0.158
0.103
29.1


28
8.93
18.1
3.23
4.53
5.8
0.194
0.107
25


29
8.53
18.5
3.23
4.97
5.73
0.188
0.12
24.6


30
10.7
22.8
3.13
4.6
5.73
0.241
0.139
30.8


31
10.3
22
3.43
4.93
6
0.244
0.124
25.5


34
7.87
20
3
4.5
5.6
0.185
0.0994
32.9


37
8.77
21.8
3
4.57
6.07
0.242
0.115
33.5





Table 37: Provided are the values of each of the parameters (as described above) measured in Sorghum accessions (Seed ID) under regular growth conditions. Growth conditions are specified in the experimental procedure section.













TABLE 38







Correlation between the expression level of selected LNU genes of some embodiments


of the invention in various tissues and the phenotypic performance under low nitrogen,


normal, cold or salinity stress conditions across Sorghum accessions
















Gene

P
Exp.
Correl.
Gene

P
Exp.
Correl.


Name
R
value
set
Set ID
Name
R
value
set
Set ID



















LNU202
0.71
3.35E−02
I
17
LNU84
0.76
4.81E−02
A
5


LNU202
0.74
1.47E−02
J
25
LNU168
0.70
3.43E−02
I
15


LNU 84
0.76
4.73E−02
A
8
LNU84
0.77
4.19E−02
A
6


LNU280
0.86
2.80E−03
C
8
LNU84
0.76
4.92E−02
A
6


LNU84
0.88
8.76E−03
A
7
LNU168
0.95
1.13E−03
A
6


LNU84
0.77
4.16E−02
A
7
LNU168
0.94
1.82E−03
A
6


LNU280
0.81
7.50E−03
C
7
LNU278
0.91
4.12E−03
A
6


LNU84
0.75
2.05E−02
L
24
LNU84
0.73
2.50E−02
L
20


LNU84
0.71
3.31E−02
L
24
LNU84
0.85
1.62E−02
A
5


LNU202
0.77
1.43E−02
L
22
LNU84
0.81
2.61E−02
A
3


LNU202
0.77
1.56E−02
L
22





Table 38. “Correl. Set ID”—correlation set ID according to the correlated parameters Table above.













TABLE 39







Correlation between the expression level of selected LNU homologous genes of some embodiments


of the invention in various tissues and the phenotypic performance under low nitrogen,


normal, cold or salinity stress conditions across Sorghum accessions




















Correl.




Correl.


Gene

P
Exp.
Set
Gene

P
Exp.
Set


Name
R
value
set
ID
Name
R
value
Set
ID



















LNU74_H173
0.96
8.89E−03
G
17
LNU271_H4
0.87
2.23E−03
L
22


LNU74_H173
0.89
4.39E−02
G
17
LNU265_H0
0.88
1.56E−03
L
22


LNU74_H173
0.96
8.89E−03
G
17
LNU265_H0
0.88
1.56E−03
L
22


LNU74_H173
0.89
4.39E−02
G
17
LNU67_H4
0.76
4.57E−02
A
5


LNU52_H6
0.73
2.48E−02
I
17
LNU74_H173
0.82
2.44E−02
A
5


LNU52_H6
0.72
2.75E−02
I
17
LNU74_H173
0.82
2.44E−02
A
5


LNU52_H6
0.73
2.48E−02
I
17
LNU13_H1
0.72
2.98E−02
C
5


LNU52_H6
0.72
2.75E−02
I
17
LNU13_H1
0.72
2.98E−02
C
5


LNU192_H3
0.82
6.46E−03
I
17
LNU45_H260
0.71
3.05E−02
C
5


LNU192_H3
0.74
2.18E−02
I
17
LNU35_H4
0.72
3.03E−02
C
5


LNU192_H3
0.82
6.46E−03
I
17
LNU35_H4
0.72
3.03E−02
C
5


LNU192_H3
0.74
2.18E−02
I
17
LNU19_H1
0.83
5.33E−03
I
15


LNU46_H60
0.76
1.85E−02
I
17
LNU263_H5
0.74
2.16E−02
I
15


LNU46_H60
0.76
1.85E−02
I
17
LNU263_H5
0.74
2.18E−02
I
15


LNU35_H4
0.81
7.57E−03
I
17
LNU263_H5
0.74
2.16E−02
I
15


LNU35_H4
0.81
7.57E−03
I
17
LNU263_H5
0.74
2.18E−02
I
15


LNU2_H4
0.72
1.93E−02
J
25
LNU45_H260
0.74
2.36E−02
C
6


LNU2_H???
0.72
1.93E−02
J
25
LNU263_H5
0.70
3.53E−02
F
31


LNU2_H4
0.72
1.93E−02
J
25
LNU263_H5
0.70
3.53E−02
F
31


LNU48_H1
0.71
2.11E−02
J
25
LNU45_H260
0.99
6.05E−04
G
10


LNU48_H1
0.71
2.11E−02
J
25
LNU45_H260
0.98
3.25E−03
G
10


LNU52_H6
0.80
1.01E−02
L
25
LNU263_H5
0.75
2.10E−02
I
10


LNU52_H6
0.77
1.61E−02
L
25
LNU263_H5
0.74
2.18E−02
I
10


LNU52_H6
0.80
1.01E−02
L
25
LNU263_H5
0.75
2.10E−02
I
10


LNU52_H6
0.77
1.61E−02
L
25
LNU263_H5
0.74
2.18E−02
I
10


LNU48_H1
0.80
2.98E−02
A
8
LNU271_H4
0.93
2.36E−04
L
19


LNU48_H1
0.80
2.98E−02
A
8
LNU271_H4
0.84
4.40E−03
L
19


LNU74_H173
0.82
2.44E−02
A
8
LNU271_H4
0.93
2.36E−04
L
19


LNU74_H173
0.82
2.46E−02
A
8
LNU271_H4
0.84
4.40E−03
L
19


LNU74_H173
0.82
2.44E−02
A
8
LNU268_H2
0.82
2.40E−02
A
1


LNU74_H173
0.82
2.46E−02
A
8
LNU268_H2
0.77
4.31E−02
A
1


LNU89_H5
0.86
1.24E−02
A
8
LNU268_H2
0.82
2.40E−02
A
1


LNU89_H5
0.79
3.64E−02
A
8
LNU268_H2
0.77
4.31E−02
A
1


LNU89_H5
0.86
1.24E−02
A
8
LNU48_H1
0.85
1.47E−02
A
1


LNU89_H5
0.79
3.64E−02
A
8
LNU48_H1
0.85
1.47E−02
A
1


LNU121_H1
0.82
6.85E−03
C
8
LNU121_H1
0.74
2.38E−02
C
1


LNU121_H1
0.71
3.24E−02
C
8
LNU121_H1
0.74
2.38E−02
C
1


LNU121_H1
0.82
6.85E−03
C
8
LNU13_H1
0.70
3.39E−02
C
1


LNU121_H1
0.71
3.24E−02
C
8
LNU13_H1
0.70
3.42E−02
C
1


LNU13_H1
0.75
2.09E−02
C
8
LNU13_H1
0.70
3.39E−02
C
1


LNU13_H1
0.72
2.77E−02
C
8
LNU13_H1
0.70
3.42E−02
C
1


LNU13_H1
0.75
2.09E−02
C
8
LNU45_H260
0.84
4.75E−03
C
1


LNU13_H1
0.72
2.77E−02
C
8
LNU45_H260
0.80
9.40E−03
C
1


LNU67_H4
0.80
1.02E−02
C
8
LNU109_H2
0.78
1.40E−02
F
27


LNU67_H4
0.74
2.39E−02
C
8
LNU109_H2
0.76
1.83E−02
F
27


LNU71_H3
0.81
7.59E−03
C
8
LNU109_H2
0.78
1.40E−02
F
27


LNU71_H3
0.81
7.59E−03
C
8
LNU109_H2
0.76
1.83E−02
F
27


LNU45_H260
0.91
5.68E−04
C
8
LNU271_H4
0.93
2.36E−04
L
20


LNU45_H260
0.89
1.29E−03
C
8
LNU271_H4
0.86
3.12E−03
L
20


LNU268_H2
0.83
2.04E−02
A
7
LNU271_H4
0.93
2.36E−04
L
20


LNU268_H2
0.77
4.14E−02
A
7
LNU271_H4
0.86
3.12E−03
L
20


LNU268_H2
0.83
2.04E−02
A
7
LNU35_H4
0.73
2.49E−02
L
20


LNU268_H2
0.77
4.14E−02
A
7
LNU35_H4
0.73
2.49E−02
L
20


LNU48_H1
0.88
9.77E−03
A
7
LNU268_H2
0.82
2.41E−02
A
2


LNU48_H1
0.88
9.77E−03
A
7
LNU268_H2
0.77
4.45E−02
A
2


LNU89_H5
0.78
4.02E−02
A
7
LNU268_H2
0.82
2.41E−02
A
2


LNU89_H5
0.78
4.02E−02
A
7
LNU268_H2
0.77
4.45E−02
A
2


LNU46_H58
0.77
4.32E−02
A
7
LNU48_H1
0.89
6.82E−03
A
2


LNU46_H58
0.77
4.32E−02
A
7
LNU48_H1
0.89
6.82E−03
A
2


LNU121_H1
0.86
2.97E−03
C
7
LNU13_H1
0.86
2.89E−03
C
2


LNU121_H1
0.76
1.81E−02
C
7
LNU13_H1
0.85
3.66E−03
C
2


LNU121_H1
0.86
2.97E−03
C
7
LNU13_H1
0.86
2.89E−03
C
2


LNU121_H1
0.76
1.81E−02
C
7
LNU13_H1
0.85
3.66E−03
C
2


LNU13_H1
0.78
1.34E−02
C
7
LNU45_H260
0.86
3.18E−03
C
2


LNU13_H1
0.78
1.39E−02
C
7
LNU45_H260
0.80
1.01E−02
C
2


LNU13_H1
0.78
1.34E−02
C
7
LNU46_H58
0.76
1.81E−02
C
2


LNU13_H1
0.78
1.39E−02
C
7
LNU46_H58
0.74
2.25E−02
C
2


LNU67_H4
0.80
1.00E−02
C
7
LNU46_H58
0.76
1.81E−02
C
2


LNU67_H4
0.74
2.34E−02
C
7
LNU46_H58
0.74
2.25E−02
C
2


LNU45_H260
0.91
7.40E−04
C
7
LNU35_H4
0.88
1.98E−03
C
2


LNU45_H260
0.90
7.93E−04
C
7
LNU35_H4
0.88
1.98E−03
C
2


LNU35_H4
0.71
3.26E−02
C
7
LNU13_H1
0.86
3.30E−03
F
28


LNU35_H4
0.71
3.26E−02
C
7
LNU13_H1
0.84
4.94E−03
F
28


LNU45_H260
0.88
4.81E−02
G
16
LNU13_H1
0.86
3.30E−03
F
28


LNU67_H4
0.79
1.14E−02
I
16
LNU13_H1
0.84
4.94E−03
F
28


LNU67_H4
0.78
1.41E−02
I
16
LNU45_H260
0.99
2.20E−03
G
11


LNU263_H5
0.71
3.36E−02
I
16
LNU45_H260
0.95
1.24E−02
G
11


LNU263_H5
0.70
3.45E−02
I
16
LNU263_H5
0.90
9.67E−04
I
11


LNU263_H5
0.71
3.36E−02
I
16
LNU263_H5
0.90
1.10E−03
I
11


LNU263_H5
0.70
3.45E−02
I
16
LNU263_H5
0.90
9.67E−04
I
11


LNU271_H4
0.93
3.24E−04
L
24
LNU263_H5
0.90
1.10E−03
I
11


LNU271_H4
0.90
1.07E−03
L
24
LNU45_H260
0.89
4.59E−02
G
11


LNU271_H4
0.93
3.24E−04
L
24
LNU263_H5
0.94
1.36E−04
I
11


LNU271_H4
0.90
1.07E−03
L
24
LNU263_H5
0.94
1.51E−04
I
11


LNU35_H4
0.75
1.92E−02
L
24
LNU263_H5
0.94
1.36E−04
I
11


LNU35_H4
0.75
1.92E−02
L
24
LNU263_H5
0.94
1.51E−04
I
11


LNU45_H260
0.93
2.14E−02
G
13
LNU268_H2
0.84
1.77E−02
A
3


LNU263_H5
0.74
2.36E−02
I
13
LNU268_H2
0.78
3.66E−02
A
3


LNU263_H5
0.74
2.38E−02
I
13
LNU268_H2
0.84
1.77E−02
A
3


LNU263_H5
0.74
2.36E−02
I
13
LNU268_H2
0.78
3.66E−02
A
3


LNU263_H5
0.74
2.38E−02
I
13
LNU48_H1
0.84
1.74E−02
A
3


LNU267_H2
0.71
3.39E−02
L
21
LNU48_H1
0.84
1.74E−02
A
3


LNU267_H2
0.71
3.39E−02
L
21
LNU13_H1
0.85
3.76E−03
C
3


LNU265_H0
0.71
3.13E−02
L
21
LNU13_H1
0.84
4.64E−03
C
3


LNU265_H0
0.71
3.13E−02
L
21
LNU13_H1
0.85
3.76E−03
C
3


LNU73_H2
0.93
2.25E−03
A
4
LNU13_H1
0.84
4.64E−03
C
3


LNU223_H6
0.95
1.29E−03
A
4
LNU45_H260
0.76
1.68E−02
C
3


LNU223_H6
0.92
3.52E−03
A
4
LNU45_H260
0.70
3.44E−02
C
3


LNU223_H6
0.95
1.29E−03
A
4
LNU46_H58
0.80
9.90E−03
C
3


LNU223_H6
0.92
3.52E−03
A
4
LNU46_H58
0.78
1.28E−02
C
3


LNU7_H125
0.79
1.17E−02
C
4
LNU46_H58
0.80
9.90E−03
C
3


LNU7_H125
0.79
1.17E−02
C
4
LNU46_H58
0.78
1.28E−02
C
3


LNU7_H124
0.79
1.17E−02
C
4
LNU35_H4
0.88
1.56E−03
C
3


LNU71_H3
0.73
2.62E−02
C
4
LNU35_H4
0.88
1.56E−03
C
3


LNU71_H3
0.73
2.62E−02
C
4
LNU19_H1
0.83
5.26E−03
L
26


LNU7_H125
0.79
1.17E−02
C
4
LNU223_H6
0.82
7.43E−03
L
26


LNU7_H124
0.79
1.17E−02
C
4
LNU223_H6
0.82
7.43E−03
L
26


LNU7_H125
0.79
1.17E−02
C
4
LNU266_H0
0.82
2.29E−02
A
9


LNU109_H2
0.78
1.29E−02
F
29
LNU266_H0
0.77
4.09E−02
A
9


LNU109_H2
0.77
1.50E−02
F
29
LNU266_H0
0.82
2.29E−02
A
9


LNU109_H2
0.78
1.29E−02
F
29
LNU266_H0
0.77
4.09E−02
A
9


LNU109_H2
0.77
1.50E−02
F
29
LNU74_H173
0.84
1.86E−02
A
9


LNU263_H5
0.90
3.77E−02
G
14
LNU74_H173
0.83
1.95E−02
A
9


LNU263_H5
0.90
3.97E−02
G
14
LNU74_H173
0.84
1.86E−02
A
9


LNU263_H5
0.90
3.77E−02
G
14
LNU74_H173
0.83
1.95E−02
A
9


LNU263_H5
0.90
3.97E−02
G
14
LNU45_H260
0.79
3.54E−02
A
9


LNU263_H5
0.74
2.18E−02
I
14
LNU45_H258
0.77
4.27E−02
A
9


LNU263_H5
0.74
2.18E−02
I
14
LNU45_H258
0.77
4.27E−02
A
9


LNU263_H5
0.74
2.18E−02
I
14
LNU46_H60
0.79
3.44E−02
A
9


LNU263_H5
0.74
2.18E−02
I
14
LNU46_H60
0.79
3.44E−02
A
9


LNU266_H0
0.72
2.97E−02
L
22
LNU266_H0
0.95
7.83E−05
C
9


LNU266_H0
0.72
2.97E−02
L
22
LNU266_H0
0.91
6.80E−04
C
9


LNU271_H4
0.88
1.94E−03
L
22
LNU266_H0
0.95
7.83E−05
C
9


LNU271_H4
0.87
2.23E−03
L
22
LNU266_H0
0.91
6.80E−04
C
9


LNU271_H4
0.88
1.94E−03
L
22
LNU265_H0
0.82
6.68E−03
D
34







LNU265_H0
0.82
6.68E−03
D
34





Table 39. “Correl. Set ID”—correlation set ID according to the correlated parameters Table above.






Example 9
Production of Maize Transcriptom and High Throughput Correlation Analysis with Yield and NUE Related Parameters Using 44K Maize Oligonucleotide Micro-Arrays
In order to produce a high throughput correlation analysis between plant phenotype and gene expression level, the present inventors utilized a maize oligonucleotide micro-array, produced by Agilent Technologies [Hypertext Transfer Protocol://World Wide Web (dot) chem. (dot) agilent (dot) com/Scripts/PDS (dot) asp?1 Page=50879]. The array oligonucleotide represents about 44,000 maize genes and transcripts. In order to define correlations between the levels of RNA expression with yield and NUE components or vigor related parameters, various plant characteristics of 12 different maize hybrids were analyzed. Among them, 10 hybrids encompassing the observed variance were selected for RNA expression analysis. The correlation between the RNA levels and the characterized parameters was analyzed using Pearson correlation test [Hypertext Transfer Protocol://World Wide Web (dot) davidmlane (dot) com/hyperstat/A34739 (dot) html].
Correlation of Maize Hybrids Across Ecotypes Grown Under Regular Growth conditions
Experimental Procedures
12 Maize hybrids were grown in 3 repetitive plots, in field. Maize seeds were planted and plants were grown in the field using commercial fertilization and irrigation protocols. In order to define correlations between the levels of RNA expression with NUE and yield components or vigor related parameters, the 12 different maize hybrids were analyzed. Among them, 10 hybrids encompassing the observed variance were selected for RNA expression analysis. The correlation between the RNA levels and the characterized parameters was analyzed using Pearson correlation test [Hypertext Transfer Protocol://World Wide Web (dot) davidmlane (dot) com/hyperstat/A34739 (dot) html].
Analyzed Sorghum tissues—All 10 selected maize hybrids were sample per each treatment. Plant tissues [Flag leaf, Flower meristem, Grain, Cobs, Internodes] growing under Normal conditions were sampled and RNA was extracted as described above. Each micro-array expression information tissue type has received a Set ID as summarized in Table 40 below.







TABLE 40







Maize transcriptom expression sets










Expression Set
Set ID







Maize field/Normal/flower meristem
A



Maize field/Normal/Ear
B



Maize field/Normal/Grain Distal
C



Maize field/Normal/Grain Basal
D



Maize field/Normal/Internode
E



Maize field/Normal/Leaf
F







Table 40: Provided are the maize transcriptom expression sets.



Leaf = the leaf below the main ear;



Flower meristem = Apical meristem following male flower initiation;



Ear = the female flower at the anthesis day.



Grain Distal = maize developing grains from the cob extreme area,



Grain Basal = maize developing grains from the cob basal area;



Internodes = internodes located above and below the main ear in the plant.






The following parameters were collected using digital imaging system:
Grain Area (cm2)—At the end of the growing period the grains were separated from the ear. A sample of  ̃200 grains were weight, photographed and images were processed using the below described image processing system. The grain area was measured from those images and was divided by the number of grains.
Grain Length and Grain width (cm)—At the end of the growing period the grains were separated from the ear. A sample of  ̃200 grains were weight, photographed and images were processed using the below described image processing system. The sum of grain lengths/or width (longest axis) was measured from those images and was divided by the number of grains.
Ear Area (cm2)—At the end of the growing period 5 ears were, photographed and images were processed using the below described image processing system. The Ear area was measured from those images and was divided by the number of Ears.
Ear Length and Ear Width (cm) At the end of the growing period 5 ears were, photographed and images were processed using the below described image processing system. The Ear length and width (longest axis) was measured from those images and was divided by the number of ears.
The image processing system was used, which consists of a personal desktop computer (Intel P4 3.0 GHz processor) and a public domain program—ImageJ 1.37, Java based image processing software, which was developed at the U.S. National Institutes of Health and is freely available on the internet at Hypertext Transfer Protocol://rsbweb (dot) nih (dot) gov/. Images were captured in resolution of 10 Mega Pixels (3888×2592 pixels) and stored in a low compression JPEG (Joint Photographic Experts Group standard) format. Next, image processing output data for seed area and seed length was saved to text files and analyzed using the JMP statistical analysis software (SAS institute).
Additional parameters were collected either by sampling 6 plants per plot or by measuring the parameter across all the plants within the plot.
Normalized Grain Weight per plant (gr.)—At the end of the experiment all ears from plots within blocks A-C were collected. 6 ears were separately threshed and grains were weighted, all additional ears were threshed together and weighted as well. The average grain weight per ear was calculated by dividing the total grain weight by number of total ears per plot (based on plot). In case of 6 ears, the total grains weight of 6 ears was divided by 6.
Ear FW (gr.)—At the end of the experiment (when ears were harvested) total and 6 selected ears per plots within blocks A-C were collected separately. The plants with (total and 6) were weighted (gr.) separately and the average ear per plant was calculated for total (Ear FW per plot) and for 6 (Ear FW per plant).
Plant height and Ear height—Plants were characterized for height at harvesting. In each measure, 6 plants were measured for their height using a measuring tape. Height was measured from ground level to top of the plant below the tassel. Ear height was measured from the ground level to the place were the main ear is located
Leaf number per plant—Plants were characterized for leaf number during growing period at 5 time points. In each measure, plants were measured for their leaf number by counting all the leaves of 3 selected plants per plot.
Relative Growth Rate was calculated using Formulas IX and X (described above).
SPAD—Chlorophyll content was determined using a Minolta SPAD 502 chlorophyll meter and measurement was performed 64 days post sowing. SPAD meter readings were done on young fully developed leaf. Three measurements per leaf were taken per plot. Data were taken after 46 and 54 days after sowing (DPS)
Dry weight per plant—At the end of the experiment (when Inflorescence were dry) all vegetative material from plots within blocks A-C were collected.
Dry weight=total weight of the vegetative portion above ground (excluding roots) after drying at 70° C. in oven for 48 hours;
Harvest Index (HI) (Maize)—The harvest index was calculated using Formula XII.

Harvest Index=Average grain dry weight per Ear/(Average vegetative dry weight per Ear+Average Ear dry weight)  Formula XII:

Percent Filled Ear [%]—it was calculated as the percentage of the Ear area with grains out of the total ear.
Cob diameter [cm]—The diameter of the cob without grains was measured using a ruler.
Kernel Row Number per Ear—The number of rows in each ear was counted.
Experimental Results
12 different maize hybrids were grown and characterized for different parameters: The average for each of the measured parameter was calculated using the JMP software (Tables 42-43) and a subsequent correlation analysis was performed (Tables 44 and 45). Results were then integrated to the database.







TABLE 41







Maize correlated parameters (vectors)










Correlations
Correlation ID














SPAD 54DPS [SPAD units]
1



SPAD 46DPS [SPAD units]
2



Growth Rate Leaf Num
3



Plant Height per Plot [cm]
4



Ear Height [cm]
5



Leaf Number per Plant [number]
6



Ear Length [cm]
7



Percent Filled Ear [%]
8



Cob Diameter [mm]
9



Kernel Row Number per Ear [number]
10



DW per Plant [gr]
11



Ear FW per Plant gr]
12



Normalized Grain Weight per plant [gr]
13



Ears FW per plot [gr]
14



Normalized Grain Weight per plot [gr]
15



Ear Area [cm2]
16



Ear Width [cm]
17



Grain Area [cm2]
18



Grain Length [cm]
19



Grain Width [cm]
20







Table 41. SPAD 46DPS and SPAD 54DPS: Chlorophyl level after 46 and 54 days after sowing (DPS).













TABLE 42







Measured parameters in Maize accessions under normal conditions


















Seed ID
1
2
3
4
5
6
7
8
9
10
11





















Line 1
54.8
55.3
0.306
287
135
11.9
20.9
80.4
28.7
16.2
656


Line 2
54.3
51.7
0.283
278
135
12
19.7
80.6
29
16.2
658


Line 3
57.2
56.4
0.221
270
116
8.4
19.1
94.3
23.8
15
472


Line 4
56
53.5
0.281
275
132
11.7
20.5
82.1
28.1
16.2
641


Line 5
59.7
55.2
0.269
238
114
11.8
21.3
92.7
25.7
15.9
581


Line 6
59.1
59.4
0.244
225
94.3
12.3
18.2
82.8
25.8
15.2
569


Line 7
58
58.5
0.244
264
121
12.4
19
73.2
26.4
16
511


Line 8
60.4
55.9
0.266
252
108
12.2
18.6
81.1
25.2
14.8
544


Line 9
54.8
53











Line 10
53.3
50











Line 11
61.1
59.7
0.301
278
112
12.6
21.7
91.6
26.7
15.4
522


Line 12
51.4
53.9
0.194
164
60.4
9.28
16.7
81.1
14.3
574
141





Table 42. Provided are the values of each of the parameters (as described above) measured in maize accessions (Seed ID) under regular growth conditions. Growth conditions are specified in the experimental procedure section.













TABLE 43







Additional measured parameters in Maize accessions under regular growth


conditions
















Seed ID
12
13
14
15
16
17
18
19
20



















Line 1
272
157
280
140
91.6
5.73
0.806
1.23
0.824


Line 2
246
141
278
154
85.1
5.58
0.753
1.17
0.81


Line 3
190
129
190
121
77.9
5.1
0.674
1.07
0.794


Line 4
262
154
288
152
90.5
5.67
0.755
1.18
0.803


Line 5
264
177
248
159
96
5.53
0.766
1.2
0.803


Line 6
178
120
176
117
72.4
5.23
0.713
1.12
0.803


Line 7
189
120
192
123
74
5.22
0.714
1.14
0.791


Line 8
197
134
205
131
76.5
5.33
0.753
1.13
0.837


Line 9











Line 10











Line 11
261
173
264
171
95.4
5.58
0.762
1.18
0.812


Line 12
54.3
143
40.8
55.2
4.12
0.796
0.921
0.675





Table 43. Provided are the values of each of the parameters (as described above) measured in maize accessions (Seed ID) under regular growth conditions. Growth conditions are specified in the experimental procedure section.













TABLE 44







Correlation between the expression level of selected LNU genes of some embodiments of the


invention in various tissues and the phenotypic performance under normal across maize accessions
















Gene

P
Exp.
Correl.
Gene

P
Exp.
Correl. Set


Name
R
value
set
Set ID
Name
R
value
set
ID



















LNU267
0.81
2.77E−02
F
8
LNU265
0.78
3.99E−02
B
17


LNU113
0.76
2.86E−02
C
9
LNU265
0.76
4.86E−02
B
5


LNU113
0.76
2.88E−02
C
9
LNU265
0.76
4.98E−02
B
18


LNU265
0.89
7.21E−03
B
8
LNU265
0.72
4.32E−02
C
15


LNU265
0.80
3.16E−02
B
20
LNU98
0.78
3.84E−02
E
11


LNU265
0.78
7.51E−03
F
2





Table 44. “Correl. Set ID”—correlation set ID according to the correlated parameters Table above.













TABLE 45







Correlation between the expression level of selected LNU homologous genes of some embodiments of the


invention in various tissues and the phenotypic performance under normal across maize accessions




















Correl.




Correl.


Gene

P
Exp.
Set
Gene

P
Exp.
Set


Name
R
value
Set
ID
Name
R
value
Set
ID



















LNU74_H112
0.84
1.67E−02
B
11
LNU34_H0
0.82
2.26E−02
F
3


LNU46_H34
0.79
3.59E−02
B
11
LNU34_H0
0.79
3.36E−02
F
3


LNU51_H1
0.76
4.84E−02
B
3
LNU34_H0
0.79
3.29E−02
E
12


LNU52_H5
0.86
1.37E−02
B
18
LNU34_H0
0.79
3.57E−02
F
3


LNU45_H173
0.79
3.53E−02
B
16
LNU115_H0
0.81
2.74E−02
B
6


LNU64_H1
0.85
1.53E−02
B
18
LNU35_H2
0.76
4.85E−02
E
12


LNU64_H1
0.85
1.54E−02
B
18
LNU74_H111
0.71
2.23E−02
F
12


LNU74_H118
0.95
3.98E−03
B
16
LNU13_H0
0.87
1.13E−02
F
12


LNU278_H2
0.84
1.89E−02
B
11
LNU45_H173
0.71
4.78E−02
E
19


LNU216_H0
0.96
2.60E−03
B
11
LNU51_H1
0.88
8.90E−03
B
6


LNU74_H110
0.84
1.83E−02
B
18
LNU74_H109
0.81
2.65E−02
B
6


LNU74_H118
0.94
5.44E−03
B
16
LNU45_H173
0.78
3.79E−02
B
6


LNU34_H0
0.76
4.59E−02
B
18
LNU279_H2
0.92
1.08E−03
C
9


LNU45_H173
0.81
4.97E−02
B
16
LNU7_H89
0.85
3.26E−02
B
6


LNU45_H173
0.77
4.28E−02
B
18
LNU52_H5
0.87
9.94E−03
B
8


LNU35_H2
0.90
5.70E−03
B
18
LNU74_H110
0.95
9.33E−04
B
8


LNU271_H3
0.81
1.46E−02
C
16
LNU7_H89
0.84
3.45E−02
B
6


LNU51_H1
0.89
1.71E−02
B
11
LNU7_H89
0.85
3.26E−02
B
6


LNU271_H3
0.76
2.73E−02
C
16
LNU7_H89
0.84
3.45E−02
B
6


LNU64_H1
0.85
1.53E−02
B
18
LNU115_H0
0.78
1.33E−02
E
6


LNU278_H2
0.90
1.50E−02
B
11
LNU74_H110
0.91
4.94E−03
B
8


LNU64_H1
0.85
1.54E−02
B
18
LNU279_H1
0.74
3.49E−02
C
9


LNU13_H0
0.77
2.47E−02
C
11
LNU34_H0
0.85
7.43E−03
C
9


LNU64_H1
0.77
4.40E−02
B
3
LNU34_H0
0.83
1.01E−02
C
9


LNU64_H1
0.76
4.82E−02
B
3
LNU52_H4
0.78
1.26E−02
E
6


LNU13_H0
0.74
3.48E−02
C
11
LNU7_H89
0.78
1.40E−02
E
6


LNU13_H0
0.74
3.53E−02
C
11
LNU7_H89
0.71
3.34E−02
E
6


LNU52_H5
0.76
2.93E−02
C
11
LNU34_H0
0.81
1.54E−02
C
9


LNU52_H5
0.73
4.07E−02
C
11
LNU7_H89
0.70
3.54E−02
E
6


LNU216_H1
0.88
8.39E−03
E
16
LNU7_H88
0.83
6.01E−03
E
6


LNU52_H5
0.86
1.39E−02
E
16
LNU74_H111
0.76
4.89E−02
F
19


LNU52_H4
0.71
4.92E−02
C
11
LNU74_H110
0.83
2.00E−02
B
8


LNU71_H0
0.78
2.30E−02
C
11
LNU7_H88
0.79
1.06E−02
E
6


LNU279_H2
0.77
4.29E−02
E
16
LNU7_H88
0.78
1.28E−02
E
6


LNU34_H0
0.95
8.43E−04
E
16
LNU74_H110
0.81
2.85E−02
B
8


LNU74_H118
0.84
3.81E−02
B
18
LNU74_H110
0.77
4.51E−02
B
8


LNU74_H111
0.71
4.86E−02
E
16
LNU52_H5
0.92
3.01E−03
B
20


LNU71_H0
0.75
3.07E−02
C
11
LNU64_H1
0.87
1.03E−02
B
20


LNU271_H3
0.74
3.77E−02
C
18
LNU7_H87
0.71
3.37E−02
E
6


LNU271_H2
0.71
4.67E−02
C
11
LNU64_H1
0.87
1.18E−02
B
20


LNU13_H0
0.78
3.87E−02
F
16
LNU74_H118
0.78
1.39E−02
E
6


LNU74_H111
0.77
4.23E−02
F
16
LNU74_H110
0.91
4.62E−03
B
20


LNU7_H87
0.84
1.84E−02
B
14
LNU74_H112
0.91
4.94E−03
B
8


LNU271_H3
0.88
3.84E−03
C
11
LNU74_H118
0.74
2.14E−02
E
6


LNU34_H0
0.73
3.96E−02
C
18
LNU74_H112
0.77
4.51E−02
B
8


LNU115_H0
0.77
1.54E−02
E
18
LNU74_H118
0.74
2.22E−02
E
6


LNU74_H118
0.74
2.38E−02
E
18
LNU74_H116
0.78
1.39E−02
E
6


LNU7_H87
0.76
4.59E−02
B
14
LNU74_H110
0.78
1.39E−02
E
6


LNU271_H3
0.78
2.29E−02
C
11
LNU74_H110
0.73
2.44E−02
E
6


LNU74_H118
0.72
2.83E−02
E
18
LNU74_H116
0.78
1.39E−02
E
6


LNU74_H109
0.82
2.31E−02
B
3
LNU74_H109
0.76
1.86E−02
E
6


LNU279_H2
0.86
6.55E−03
C
11
LNU74_H109
0.73
2.54E−02
E
6


LNU7_H87
0.76
4.79E−02
B
14
LNU74_H113
0.78
1.39E−02
E
6


LNU34_H0
0.82
1.35E−02
C
11
LNU74_H112
0.78
1.39E−02
E
6


LNU85_H2
0.71
3.12E−02
E
18
LNU74_H112
0.73
2.44E−02
E
6


LNU7_H87
0.84
1.84E−02
B
14
LNU74_H110
0.77
4.18E−02
B
20


LNU34_H0
0.81
1.51E−02
C
11
LNU74_H110
0.77
4.27E−02
B
20


LNU34_H0
0.78
2.29E−02
C
11
LNU74_H110
0.77
4.39E−02
B
20


LNU271_H3
0.84
4.49E−03
E
11
LNU7_H89
0.78
1.40E−02
E
6


LNU271_H3
0.77
1.58E−02
E
11
LNU74_H112
0.77
4.39E−02
B
20


LNU7_H87
0.76
4.59E−02
B
14
LNU7_H89
0.71
3.34E−02
E
6


LNU7_H87
0.76
4.79E−02
B
14
LNU7_H89
0.70
3.54E−02
E
6


LNU69_H2
0.76
4.75E−02
E
11
LNU7_H88
0.83
6.01E−03
E
6


LNU74_H111
0.82
2.44E−02
E
11
LNU7_H88
0.79
1.06E−02
E
6


LNU45_H173
0.87
1.10E−02
B
14
LNU7_H88
0.78
1.28E−02
E
6


LNU279_H1
0.88
2.09E−02
B
10
LNU7_H87
0.71
3.37E−02
E
6


LNU45_H173
0.85
1.45E−02
B
14
LNU45_H169
0.80
1.01E−02
E
6


LNU45_H173
0.84
1.78E−02
B
14
LNU45_H169
0.78
1.32E−02
E
6


LNU76_H37
0.76
4.93E−02
E
18
LNU45_H168
0.82
6.36E−03
E
6


LNU34_H0
0.84
3.62E−02
B
9
LNU51_H1
0.80
3.24E−02
E
6


LNU74_H111
0.76
4.94E−02
E
11
LNU34_H0
0.92
2.97E−03
B
8


LNU76_H38
0.76
4.93E−02
E
18
LNU279_H2
0.83
1.99E−02
E
6


LNU74_H118
0.96
2.95E−03
B
14
LNU34_H0
0.75
3.22E−02
E
6


LNU74_H118
0.94
5.02E−03
B
14
LNU34_H0
0.86
1.41E−02
B
20


LNU46_H35
0.83
1.99E−02
E
11
LNU34_H0
0.81
2.64E−02
B
20


LNU45_H173
0.89
1.70E−02
B
14
LNU34_H0
0.76
4.86E−02
B
20


LNU45_H173
0.89
1.77E−02
B
14
LNU35_H2
0.93
2.72E−03
B
20


LNU279_H2
0.84
1.76E−02
E
18
LNU64_H1
0.87
1.03E−02
B
20


LNU34_H0
0.89
6.53E−03
E
18
LNU271_H3
0.88
3.49E−03
E
9


LNU45_H173
0.92
3.19E−03
B
3
LNU64_H1
0.87
1.18E−02
B
20


LNU48_H0
0.75
1.31E−02
F
11
LNU271_H3
0.80
1.78E−02
E
9


LNU115_H0
0.83
2.14E−02
F
11
LNU17_H1
0.84
3.53E−02
B
20


LNU45_H173
0.90
1.54E−02
B
9
LNU2_H3
0.81
4.88E−02
B
20


LNU45_H173
0.83
3.90E−02
B
14
LNU48_H0
0.93
7.37E−03
B
20


LNU35_H2
0.88
2.05E−02
B
14
LNU25_H0
0.87
2.57E−02
B
20


LNU271_H3
0.88
3.73E−03
C
14
LNU223_H3
0.74
3.56E−02
E
9


LNU271_H3
0.79
1.87E−02
C
14
LNU25_H0
0.85
3.39E−02
B
20


LNU35_H2
0.79
3.62E−02
E
18
LNU279_H0
0.87
2.42E−02
B
20


LNU279_H2
0.78
2.30E−02
C
14
LNU279_H0
0.86
2.94E−02
B
20


LNU216_H1
0.94
1.76E−03
E
14
LNU279_H2
0.92
8.63E−03
B
20


LNU52_H5
0.76
4.97E−02
E
14
LNU34_H0
0.89
6.68E−03
B
8


LNU34_H0
0.88
8.34E−03
E
14
LNU279_H1
0.92
8.63E−03
B
20


LNU13_H0
0.85
1.65E−02
F
14
LNU34_H0
0.81
2.75E−02
B
8


LNU45_H173
0.78
3.87E−02
B
3
LNU239_H6
0.75
3.13E−02
C
20


LNU74_H111
0.81
2.78E−02
F
18
LNU35_H2
0.94
1.48E−03
B
8


LNU45_H173
0.77
4.38E−02
B
3
LNU85_H2
0.84
1.89E−02
B
8


LNU84_H0
0.76
4.64E−02
F
14
LNU239_H6
0.75
3.17E−02
C
20


LNU84_H0
0.76
4.64E−02
F
14
LNU85_H2
0.78
1.38E−02
E
20


LNU64_H1
0.87
1.14E−02
B
5
LNU85_H2
0.77
4.20E−02
B
8


LNU64_H1
0.81
2.84E−02
B
5
LNU85_H2
0.76
1.72E−02
E
20


LNU7_H87
0.79
3.46E−02
B
5
LNU76_H37
0.78
3.68E−02
E
20


LNU35_H2
0.76
4.90E−02
F
18
LNU19_H0
0.82
4.69E−02
B
8


LNU52_H5
0.79
3.62E−02
B
19
LNU279_H2
0.77
4.33E−02
E
20


LNU7_H87
0.76
4.94E−02
B
5
LNU74_H111
0.85
3.33E−02
B
8


LNU64_H1
0.78
3.97E−02
B
19
LNU45_H169
0.91
1.17E−02
B
8


LNU7_H87
0.79
3.46E−02
B
5
LNU239_H5
0.90
1.43E−02
B
8


LNU7_H87
0.76
4.94E−02
B
5
LNU34_H0
0.77
4.20E−02
E
20


LNU35_H2
0.78
3.76E−02
B
3
LNU64_H1
0.70
3.41E−02
E
8


LNU34_H0
0.80
2.99E−02
B
5
LNU35_H2
0.78
3.92E−02
E
20


LNU34_H0
0.77
4.31E−02
B
5
LNU153_H3
0.77
4.26E−02
E
20


LNU35_H2
0.77
4.28E−02
B
5
LNU19_H0
0.75
3.05E−02
E
20


LNU64_H1
0.87
1.14E−02
B
5
LNU216_H0
0.80
1.78E−02
E
20


LNU64_H1
0.81
2.84E−02
B
5
LNU67_H2
0.86
6.40E−03
E
20


LNU64_H1
0.78
4.04E−02
B
19
LNU271_H2
0.87
4.49E−03
E
20


LNU35_H2
0.85
3.04E−02
B
5
LNU279_H2
0.89
3.36E−03
E
20


LNU74_H110
0.75
4.99E−02
B
19
LNU279_H2
0.72
4.47E−02
E
20


LNU13_H0
0.72
4.54E−02
C
5
LNU279_H1
0.72
4.47E−02
E
20


LNU45_H173
0.82
2.26E−02
B
19
LNU223_H4
0.72
1.91E−02
F
20


LNU35_H2
0.84
1.78E−02
B
19
LNU76_H37
0.70
2.35E−02
F
6


LNU279_H2
0.90
2.37E−03
C
5
LNU74_H111
0.81
2.70E−02
F
20


LNU46_H34
0.81
1.46E−02
C
5
LNU35_H2
0.79
3.57E−02
F
20


LNU34_H0
0.82
2.36E−02
E
5
LNU52_H5
0.76
4.89E−02
B
15


LNU64_H1
0.78
3.97E−02
B
19
LNU74_H110
0.80
3.12E−02
B
15


LNU35_H2
0.93
2.24E−03
E
5
LNU222_H2
0.74
2.37E−02
E
8


LNU64_H1
0.78
4.04E−02
B
19
LNU45_H173
0.76
4.93E−02
B
15


LNU74_H111
0.78
4.02E−02
F
5
LNU64_H1
0.70
3.41E−02
E
8


LNU64_H1
0.77
4.40E−02
B
3
LNU35_H2
0.76
4.79E−02
B
15


LNU64_H1
0.76
4.82E−02
B
3
LNU52_H5
0.79
3.46E−02
E
8


LNU74_H118
0.94
4.87E−03
B
19
LNU74_H118
0.91
1.27E−02
B
15


LNU35_H2
0.78
3.87E−02
F
5
LNU74_H118
0.90
1.51E−02
B
15


LNU7_H87
0.77
4.50E−02
B
7
LNU45_H169
0.86
2.93E−02
B
15


LNU74_H118
0.93
6.77E−03
B
19
LNU34_H0
0.77
4.47E−02
E
8


LNU7_H87
0.77
4.50E−02
B
7
LNU45_H169
0.84
3.68E−02
B
15


LNU45_H173
0.88
2.14E−02
B
19
LNU45_H168
0.88
2.11E−02
B
15


LNU45_H173
0.76
4.71E−02
B
7
LNU35_H2
0.80
2.92E−02
E
8


LNU74_H118
0.88
2.01E−02
B
7
LNU216_H1
0.78
2.36E−02
E
8


LNU74_H118
0.88
2.09E−02
B
7
LNU216_H0
0.73
4.00E−02
C
15


LNU45_H168
0.88
2.20E−02
B
7
LNU271_H3
0.72
4.46E−02
C
15


LNU271_H3
0.80
1.70E−02
C
7
LNU51_H1
0.71
4.94E−02
E
8


LNU271_H3
0.76
2.93E−02
C
7
LNU52_H5
0.75
3.18E−02
E
8


LNU216_H1
0.93
2.42E−03
E
7
LNU76_H38
0.70
2.35E−02
F
6


LNU45_H173
0.87
2.25E−02
B
19
LNU271_H3
0.71
4.85E−02
C
15


LNU52_H5
0.87
1.07E−02
E
7
LNU216_H1
0.79
3.42E−02
E
15


LNU279_H2
0.76
4.74E−02
E
7
LNU69_H2
0.71
5.00E−02
E
8


LNU34_H0
0.92
3.25E−03
E
7
LNU52_H5
0.87
1.18E−02
E
15


LNU45_H173
0.83
4.10E−02
B
19
LNU45_H170
0.72
4.38E−02
E
8


LNU45_H173
0.81
1.49E−02
E
7
LNU76_H37
0.77
4.30E−02
E
15


LNU45_H173
0.74
3.39E−02
E
7
LNU45_H173
0.72
4.38E−02
E
8


LNU45_H173
0.73
4.07E−02
E
7
LNU76_H38
0.77
4.30E−02
E
15


LNU13_H0
0.84
1.76E−02
F
7
LNU74_H110
0.79
3.47E−02
F
8


LNU52_H5
0.77
4.42E−02
B
17
LNU74_H110
0.76
4.66E−02
F
8


LNU64_H1
0.85
1.52E−02
B
17
LNU279_H2
0.81
2.68E−02
E
15


LNU64_H1
0.84
1.84E−02
B
17
LNU34_H0
0.97
2.32E−04
E
15


LNU74_H110
0.80
3.12E−02
B
17
LNU45_H173
0.74
3.52E−02
E
15


LNU74_H118
0.87
2.34E−02
B
3
LNU74_H111
0.81
2.82E−02
F
15


LNU34_H0
0.77
4.46E−02
B
17
LNU35_H2
0.78
3.75E−02
F
15


LNU74_H118
0.85
3.35E−02
B
3
LNU52_H5
0.77
4.49E−02
B
13


LNU35_H2
0.91
1.16E−02
B
19
LNU64_H1
0.78
4.03E−02
B
13


LNU45_H173
0.81
2.74E−02
B
17
LNU74_H111
0.88
8.99E−03
F
8


LNU35_H2
0.89
6.82E−03
B
17
LNU74_H110
0.84
1.89E−02
B
13


LNU64_H1
0.85
1.52E−02
B
17
LNU74_H111
0.78
3.68E−02
F
8


LNU7_H87
0.77
2.69E−02
C
19
LNU35_H2
0.88
9.78E−03
F
8


LNU7_H87
0.75
3.33E−02
C
19
LNU45_H173
0.78
3.75E−02
B
13


LNU64_H1
0.84
1.84E−02
B
17
LNU45_H173
0.79
3.34E−02
B
10


LNU51_H1
0.82
4.40E−02
B
17
LNU35_H2
0.84
1.89E−02
B
13


LNU74_H118
0.94
5.68E−03
B
17
LNU7_H89
0.82
4.69E−02
B
10


LNU7_H87
0.77
2.69E−02
C
19
LNU64_H1
0.78
4.03E−02
B
13


LNU7_H87
0.75
3.33E−02
C
19
LNU74_H110
0.94
5.95E−03
B
10


LNU271_H3
0.87
4.80E−03
C
19
LNU74_H112
0.94
5.95E−03
B
10


LNU271_H3
0.82
1.30E−02
C
19
LNU74_H118
0.89
1.81E−02
B
13


LNU74_H118
0.92
8.82E−03
B
17
LNU74_H118
0.87
2.55E−02
B
13


LNU45_H173
0.93
7.66E−03
B
17
LNU216_H0
0.73
3.89E−02
C
13


LNU45_H173
0.92
8.24E−03
B
17
LNU71_H0
0.75
3.33E−02
C
13


LNU45_H173
0.87
2.30E−02
B
17
LNU271_H3
0.81
1.58E−02
C
13


LNU35_H2
0.85
3.01E−02
B
17
LNU7_H89
0.82
4.69E−02
B
10


LNU13_H0
0.71
4.87E−02
C
17
LNU271_H3
0.86
6.62E−03
C
10


LNU7_H87
0.72
4.19E−02
C
17
LNU271_H3
0.78
2.12E−02
C
10


LNU71_H0
0.77
2.47E−02
C
17
LNU271_H3
0.78
2.34E−02
C
13


LNU71_H0
0.75
3.28E−02
C
17
LNU279_H2
0.77
2.46E−02
C
10


LNU7_H87
0.72
4.19E−02
C
17
LNU216_H1
0.81
2.65E−02
E
10


LNU271_H3
0.89
2.99E−03
C
17
LNU52_H5
0.87
1.14E−02
E
13


LNU271_H3
0.77
2.46E−02
C
17
LNU279_H2
0.86
1.37E−02
E
13


LNU279_H2
0.87
4.77E−03
C
17
LNU48_H0
0.72
2.73E−02
F
9


LNU34_H0
0.72
4.57E−02
C
17
LNU64_H1
0.84
1.85E−02
B
4


LNU115_H0
0.71
3.22E−02
E
17
LNU7_H89
0.73
1.58E−02
F
2


LNU115_H0
0.79
1.10E−02
E
19
LNU74_H110
0.73
1.55E−02
F
2


LNU74_H118
0.72
2.72E−02
E
19
LNU84_H0
0.77
4.38E−02
E
10


LNU52_H5
0.77
4.15E−02
E
17
LNU74_H111
0.74
2.28E−02
F
9


LNU279_H2
0.82
2.45E−02
E
17
LNU34_H0
0.84
1.72E−02
E
10


LNU34_H0
0.92
3.49E−03
E
17
LNU74_H111
0.72
3.03E−02
F
9


LNU74_H118
0.71
3.14E−02
E
19
LNU64_H1
0.77
4.22E−02
B
4


LNU35_H2
0.86
1.39E−02
E
17
LNU74_H112
0.73
1.55E−02
F
2


LNU74_H111
0.83
2.11E−02
F
17
LNU74_H110
0.87
1.17E−02
B
4


LNU35_H2
0.77
4.11E−02
F
17
LNU74_H110
0.76
4.57E−02
B
4


LNU76_H37
0.76
4.52E−02
E
19
LNU7_H89
0.73
1.58E−02
F
2


LNU7_H87
0.91
4.98E−03
B
12
LNU45_H169
0.72
2.73E−02
F
9


LNU7_H87
0.86
1.38E−02
B
12
LNU280_H1
0.78
8.26E−03
F
2


LNU7_H87
0.83
1.96E−02
B
12
LNU34_H0
0.95
9.71E−04
E
13


LNU7_H87
0.91
4.98E−03
B
12
LNU34_H0
0.82
2.41E−02
B
4


LNU7_H87
0.86
1.38E−02
B
12
LNU115_H0
0.84
3.61E−02
F
9


LNU13_H0
0.82
1.27E−02
C
9
LNU34_H0
0.80
3.12E−02
B
4


LNU13_H0
0.79
1.89E−02
C
9
LNU35_H2
0.83
2.10E−02
B
4


LNU13_H0
0.79
1.91E−02
C
9
LNU85_H2
0.77
4.39E−02
B
4


LNU13_H0
0.77
2.44E−02
C
3
LNU84_H0
0.77
4.38E−02
E
10


LNU7_H87
0.83
1.96E−02
B
12
LNU51_H1
0.77
4.46E−02
F
6


LNU13_H0
0.77
2.56E−02
C
3
LNU74_H111
0.74
1.41E−02
F
10


LNU13_H0
0.76
2.80E−02
C
3
LNU74_H111
0.72
1.93E−02
F
10


LNU45_H173
0.91
4.15E−03
B
12
LNU64_H1
0.84
1.85E−02
B
4


LNU45_H173
0.91
4.63E−03
B
12
LNU84_H0
0.79
3.42E−02
F
10


LNU45_H173
0.88
9.87E−03
B
12
LNU64_H1
0.77
4.22E−02
B
4


LNU74_H118
0.91
1.15E−02
B
12
LNU35_H2
0.94
5.10E−03
B
4


LNU74_H118
0.89
1.69E−02
B
12
LNU7_H89
0.81
4.97E−02
F
9


LNU45_H173
0.92
9.33E−03
B
12
LNU279_H2
0.78
2.37E−02
C
4


LNU45_H173
0.91
1.10E−02
B
12
LNU84_H0
0.79
3.42E−02
F
10


LNU52_H5
0.83
1.06E−02
C
9
LNU19_H0
0.91
1.21E−02
B
8


LNU52_H5
0.83
1.10E−02
C
9
LNU46_H34
0.82
1.23E−02
C
4


LNU52_H5
0.77
2.55E−02
C
3
LNU85_H2
0.72
3.04E−02
E
4


LNU45_H173
0.87
2.48E−02
B
12
LNU45_H169
0.90
1.43E−02
B
8


LNU52_H4
0.78
2.19E−02
C
3
LNU52_H5
0.83
2.02E−02
E
4


LNU74_H111
0.72
4.46E−02
C
3
LNU32_H0
0.83
1.97E−02
E
4


LNU52_H4
0.75
3.11E−02
C
9
LNU45_H169
0.89
1.83E−02
B
8


LNU271_H2
0.72
4.57E−02
C
3
LNU67_H2
0.78
3.74E−02
F
6


LNU76_H38
0.76
4.52E−02
E
19
LNU35_H2
0.78
3.76E−02
E
13


LNU279_H2
0.83
2.06E−02
E
19
LNU64_H1
0.73
1.70E−02
F
13


LNU35_H2
0.90
1.55E−02
B
12
LNU32_H0
0.83
2.16E−02
E
4


LNU271_H3
0.79
1.99E−02
C
3
LNU45_H169
0.88
2.01E−02
B
8


LNU13_H0
0.72
4.60E−02
C
12
LNU74_H111
0.78
3.76E−02
F
1


LNU279_H2
0.85
7.52E−03
C
3
LNU45_H168
0.82
4.55E−02
B
8


LNU34_H0
0.81
1.44E−02
C
3
LNU280_H1
0.75
3.04E−02
C
8


LNU71_H0
0.77
2.60E−02
C
12
LNU64_H1
0.73
1.70E−02
F
13


LNU34_H0
0.75
3.24E−02
C
3
LNU7_H89
0.81
4.97E−02
F
9


LNU34_H0
0.71
4.66E−02
C
3
LNU52_H5
0.76
4.59E−02
E
8


LNU74_H111
0.72
4.32E−02
C
9
LNU34_H0
0.82
2.30E−02
F
6


LNU115_H0
0.82
7.10E−03
E
3
LNU34_H0
0.77
4.48E−02
E
4


LNU34_H0
0.94
1.70E−03
E
19
LNU34_H0
0.77
4.23E−02
F
6


LNU115_H0
0.78
2.16E−02
E
19
LNU34_H0
0.76
4.74E−02
F
6


LNU71_H0
0.75
3.37E−02
C
12
LNU35_H2
0.92
3.67E−03
E
4


LNU271_H2
0.77
2.49E−02
C
9
LNU74_H110
0.83
2.20E−02
F
4


LNU74_H118
0.72
2.92E−02
E
3
LNU76_H37
0.76
2.84E−02
E
8


LNU74_H118
0.70
3.55E−02
E
3
LNU74_H110
0.76
4.91E−02
F
4


LNU271_H3
0.85
7.04E−03
C
12
LNU45_H173
1.00
2.63E−05
F
9


LNU279_H2
0.95
1.30E−03
E
3
LNU45_H173
0.99
9.74E−05
F
9


LNU34_H0
0.80
3.18E−02
E
3
LNU45_H173
0.97
1.64E−03
F
9


LNU74_H110
0.85
7.58E−03
E
19
LNU74_H111
0.93
2.57E−03
F
4


LNU271_H3
0.72
4.44E−02
C
12
LNU76_H38
0.76
2.84E−02
E
8


LNU13_H0
0.80
3.08E−02
F
3
LNU74_H111
0.87
1.14E−02
F
4


LNU271_H3
0.82
1.26E−02
C
9
LNU74_H111
0.87
1.12E−02
F
13


LNU74_H112
0.85
7.58E−03
E
19
LNU35_H2
0.94
1.58E−03
F
4


LNU279_H2
0.92
1.15E−03
C
12
LNU2_H3
0.81
2.74E−02
F
8


LNU74_H111
0.81
2.57E−02
F
3
LNU271_H3
0.82
2.53E−02
F
8


LNU216_H1
0.89
7.80E−03
E
12
LNU35_H2
0.83
2.02E−02
F
13





Table 45. “Correl. Set ID”—correlation set ID according to the correlated parameters Table above.






Example 10
Production of Tomato Transcription and High Throughput Correlation Analysis Using 44K Tomato Oligonucleotide Micro-Array
In order to produce a high throughput correlation analysis between NUE related phenotypes and gene expression, the present inventors utilized a Tomato oligonucleotide micro-array, produced by Agilent Technologies [Hypertext Transfer Protocol://World Wide Web (dot) chem. (dot) agilent (dot) com/Scripts/PDS (dot) asp?1 Page=50879]. The array oligonucleotide represents about 44,000 Tomato genes and transcripts. In order to define correlations between the levels of RNA expression with NUE, ABST, yield components or vigor related parameters various plant characteristics of 18 different Tomato varieties were analyzed. Among them, 10 varieties encompassing the observed variance were selected for RNA expression analysis. The correlation between the RNA levels and the characterized parameters was analyzed using Pearson correlation test [Hypertext Transfer Protocol://World Wide Web (dot) davidmlane (dot) com/hyperstat/A34739 (dot) html].
Correlation of Tomato Varieties Across Ecotypes Grown Under Low Nitrogen, Drought and Regular Growth Conditions
Experimental Procedures:
Tomato varieties were grown in 3 repetitive blocks, each containing 6 plants per plot were grown at net house. Briefly, the growing protocol was as follows:
1. Regular growth conditions: Tomato varieties were grown under normal conditions (4-6 Liters/m2 of water per day and fertilized with NPK as recommended in protocols for commercial tomato production).
2. Low Nitrogen fertilization conditions: Tomato varieties were grown under normal conditions (4-6 Liters/m2 per day and fertilized with NPK as recommended in protocols for commercial tomato production) until flower stage. At this time, Nitrogen fertilization was stopped.
3. Drought stress: Tomato variety was grown under normal conditions (4-6 Liters/m2 per day) until flower stage. At this time, irrigation was reduced to 50% compared to normal conditions. Plants were phenotyped on a daily basis following the standard descriptor of tomato (Table 47). Harvest was conducted while 50% of the fruits were red (mature). Plants were separated to the vegetative part and fruits, of them, 2 nodes were analyzed for additional inflorescent parameters such as size, number of flowers, and inflorescent weight. Fresh weight of all vegetative material was measured. Fruits were separated to colors (red vs. green) and in accordance with the fruit size (small, medium and large). Next, analyzed data was saved to text files and processed using the JMP statistical analysis software (SAS institute). Data parameters collected are summarized in Table 47, hereinbelow.
Analyzed Sorghum tissues—Two tissues at different developmental stages [flower and leaf], representing different plant characteristics, were sampled and RNA was extracted as described above. For convenience, each micro-array expression information tissue type has received a Set ID as summarized in Table 46 below.







TABLE 46







Tomato transcriptom expression sets










Expression Set
Set ID







Leaf grown under Normal Conditions
A



Leaf grown under 50% Irrigation
B



Flower grown under Normal Conditions
C



Flower grown under 50% Irrigation
D



Leaf grown under Low Nitrogen
E



Flower grown under Low Nitrogen
F







Table 46. Provided are the identification (ID) letters of each of the tomato expression sets.






The average for each of the measured parameter was calculated using the JMP software and values are summarized in Tables 48, 49 and 50 below. Subsequent correlation analysis was conducted (Tables 51-52) with the correlation coefficient (R) and the p-values. Results were integrated to the database.







TABLE 47







Tomato correlated parameters (vectors)










Correlation
Correlation ID














Fruit Yield/Plant Drought [gr.]
1



FW/Plant Drought [gr.]
2



average red fruit weight Drought [gr.]
3



RWC Drought [%]
4



Num of flowers (Drought) [number]
5



Weight flower clusters (Drought) [gr.]
6



Fruit yield/Plant (Normal) [gr.]
7



FW/Plant (Normal) [gr.]
8



average red fruit weight (Normal) [gr.]
9



SPAD (Normal) [SPAD unit]
10



RWC (Normal) [%]
11



SPAD 100% RWC (Normal)
12



No flowers (Normal) [number]
13



Weight Flower clusters (Normal) [gr.]
14



Fruit Yield/Plant (NUE) [gr.]
15



FW/Plant (NUE) [gr.]
16



average red fruit weight (NUE) [gr.]
17



SPAD NUE [SPAD unit]
18



RWC NUE [%]
19



SPAD 100% RWC (NUE) [SPAD unit]
20



No flowers (NUE) [number]
21



Weight clusters (flowers) (NUE) [gr.]
22







Table 47. Provided are the tomato correlated parameters.






Fruit Yield (grams)—At the end of the experiment [when 50% of the fruit were ripe (red)] all fruits from plots within blocks A-C were collected. The total fruits were counted and weighted. The average fruits weight was calculated by dividing the total fruit weight by the number of fruits.
Plant Fresh Weight (grams)—At the end of the experiment [when 50% of the fruit were ripe (red)] all plants from plots within blocks A-C were collected. Fresh weight was measured (grams).
Inflorescence Weight (grams)—At the end of the experiment [when 50% of the fruits were ripe (red)] two Inflorescence from plots within blocks A-C were collected. The Inflorescence weight (gr.) and number of flowers per inflorescence were counted.
SPAD—Chlorophyll content was determined using a Minolta SPAD 502 chlorophyll meter and measurement was performed at time of flowering. SPAD meter readings were done on young fully developed leaf. Three measurements per leaf were taken per plot.
Water use efficiency (WUE)—can be determined as the biomass produced per unit transpiration. To analyze WUE, leaf relative water content was measured in control and transgenic plants. Fresh weight (FW) was immediately recorded; then leaves were soaked for 8 hours in distilled water at room temperature in the dark, and the turgid weight (TW) was recorded. Total dry weight (DW) was recorded after drying the leaves at 60° C. to a constant weight. Relative water content (RWC) was calculated according to the following Formula I [(FW−DW/TW−DW)×100] as described above.
Plants that maintain high relative water content (RWC) compared to control lines were considered more tolerant to drought than those exhibiting reduced relative water content
Experimental Results







TABLE 48







Measured parameters in Tomato accessions under drought conditions













Seed ID
1
2
3
4
5
6
















612
0.467
2.62
0.00925
72.1
16.7
0.368


613
0.483
1.09
0.195
74.5
6.5
0.407


614
0.629
1.85
0.209
65.3
15.7
0.325


616
0.347
2.22
0.00467
72.2
20.3
0.288


617
2.04
2.63
0.102
66.1
11.7
0.551


618
0.25
2.71
0.00193
68.3
25.3
0.311


620
0.045
3.41
0.0346
78.1
29.7
0.445


621
0.453
2.11
0.00627
18.5
17.3
0.555


622
0.292
1.95
0.00527
73.2
14.7
0.304


623
1.02
1.76
0.00487
62.5
29.7
0.315


624
0.6
1.72
0.0052
67.2
15
0.308


625
0.494
1.92
0.012
75.8
10.3
0.311


626
0.272
2.21
0.00451
62.8
18.3
8.36


627
0.679
3.73
0.00632
70.7
12
0.288


628
0.14
0.754
0.303
55.8
20.3
0.342


629
0.529
1.76
0.138
75.2
12.7
0.441


630
0.554
0.626
0.0405
63.7
12.7
0.268


631
0.414
1.11
0.0885
62.3
11.3
0.426





Table 48: Provided are the values of each of the parameters (as described above) measured in Sorghum accessions (Seed ID) under drought growth conditions. Growth conditions are specified in the experimental procedure section.













TABLE 49







Measured parameters in Tomato accessions under normal conditions















Seed ID
7
8
9
10
11
12
13
14


















612
0.826
1.53
0.0479
49.7
72.8
36.2
5.67
1.17


613
0.342
3.17
0.00799
37.2
76.5
28.4
19.3
0.342


614
0.494
3.02
0.00823
55.8
64.3
35.9
6.33
0.693


616
0.121
0.844
0.286
46.4
67.1
31.1
7.67
56.3


617
0.487
2.24
0.00503
48.2
54.8
26.4
9.67
0.44


618
0.454
1.98
0.0541
43.4
77.6
33.7
8.33
11.3


620
0.529
0.848
0.231
42.9
58.2
25
5
0.79


621
0.44
2.09
0.29
53.3
66.5
35.5
8.33
0.577


622
0.21
3.21
0.0061
58.5
64.7
37.9
10
0.73


623
0.31
2.75
0.0066
51.1
75.2
38.4
7
0.833


624
0.662
1.81
0.0577
40
66.2
26.5
9
0.86


625
0.189
3.77
0.007
47.6
63.2
30.1
8
0.5


626
0.852
1.89
0.0264
57.9
56.8
32.9
5.33
1.02


627
0.273
1.93
0.261
48.3
36
17.4
8
0.7


628
0.347
2.14
0.0289
43.6
77.6
33.8
7.67
0.377


629
0.327
1.65
0.00493
54.5
100
54.5
9
0.66


630
0.314
3.01
0.00343
41.6
63.2
26.3
10.7
0.7


631
0.291
2.29
0.00887
59.1
75.1
44.4
9
0.327





Table 49: Provided are the values of each of the parameters (as described above) measured in Sorghum accessions (Seed ID) under normal growth conditions. Growth conditions are specified in the experimental procedure section.













TABLE 50







Measured parameters in Tomato accessions under low nitrogen conditions















Seed Id
15
16
17
18
19
20
21
22


















612
0.406
4.04
0.0239
38.4
74.1
28.5
19
0.533


613
0.66
1.21
0.191
39.4
99.1
39
5.33
0.367


614
0.477
2.25
0.00647
47.5
69.5
33
9
0.307


616
0.458
2.54
0.0053
37
63.2
23.4
13
0.35


617
1.35
1.85
0.0963
44.6
77.4
34.5
10.7
0.473


618
0.354
3.06
0.0044
41.7
77.9
32.5
16.7
0.249


620
0.00889
3.13
0.00553
34.4
80.5
27.7
6
0.293


621
0.509
2.54
0.00747
50
67.4
33.7
16
0.467


622
0.436
1.84
0.0058
44.7
67.2
30
15
0.4


623
0.468
1.52
0.0127
53.7
66.1
35.5
6
0.303


624
1.59
1.91
0.0212
35.7
69.6
24.8
17
0.82


625
0.388
1.86
0.0052
58.8
69.3
40.8
13
0.4


626
0.323
2.47
0.00573
47.5
100
47.5
8.67
0.347


627
0.449
2.62
0.0475
45.2
57.7
26.1
9.33
0.428


628
0.143
1.08
0.357
39
90.8
35.4
12.7
0.353


629
0.396
1.17
0.0367
45
68
30.6
6.67
0.447


630
1.44
0.921
0.626
65.3
59.6
39
9.33
0.283


631
0.495
1.09
1.7
72.2
37.5
0.878
0.47
0.889





Table 50: Provided are the values of each of the parameters (as described above) measured in Sorghum accessions (Seed ID) under low nitrogen growth conditions. Growth conditions are specified in the experimental procedure section.













TABLE 51







Correlation between the expression level of selected LNU genes of some embodiments of the


invention in various tissues and the phenotypic performance under low nitrogen,


normal or drought stress conditions across Tomato accessions
















Gene

P
Exp.
Correl.
Gene

P
Exp.
Correl.


Name
R
value
set
Set ID
Name
R
value
set
Set ID



















LNU20
0.85
1.78E−03
E
20
LNU288
0.87
1.10E−03
A
14


LNU245
0.95
7.41E−05
F
17
LNU288
0.83
2.98E−03
A
9


LNU245
0.84
2.23E−03
A
13
LNU288
0.83
3.30E−03
A
9


LNU245
0.73
1.66E−02
F
22
LNU288
0.80
5.50E−03
B
8


LNU246
0.71
2.25E−02
B
5
LNU288
0.77
9.78E−03
A
14


LNU29
1.00
5.21E−10
A
14
LNU288
0.72
1.90E−02
B
8


LNU229
0.74
1.50E−02
A
9
LNU289
0.82
6.93E−03
E
17


LNU229
0.72
1.80E−02
A
14
LNU289
0.75
1.22E−02
B
6


LNU200
0.86
1.29E−03
C
14
LNU289
0.75
1.24E−02
A
16







LNU289
0.70
3.52E−02
E
17





Table 51. “Correl. Set ID”—correlation set ID according to the correlated parameters Table above.













TABLE 52







Correlation between the expression level of selected LNU homologous genes of some


embodiments of the invention in various tissues and the phenotypic performance


under low nitrogen, normal or drought stress conditions across Tomato accessions




















Correl.




Correl.


Gene

P
Exp.
Set
Gene

P
Exp.
Set


Name
R
value
Set
ID
Name
R
value
Set
ID



















LNU128_H17
0.75
1.32E−02
A
10
LNU45_H302
0.80
5.89E−03
F
21


LNU128_H17
0.73
1.76E−02
A
10
LNU45_H302
0.76
1.08E−02
F
21


LNU46_H77
0.78
7.55E−03
F
18
LNU45_H302
0.76
1.14E−02
F
21


LNU74_H204
0.71
2.22E−02
D
2
LNU45_H301
0.76
1.14E−02
F
21


LNU45_H302
0.71
2.28E−02
D
2
LNU45_H300
0.82
3.93E−03
D
4


LNU74_H203
0.84
2.34E−03
F
21
LNU45_H300
0.72
1.87E−02
D
4


LNU74_H204
0.74
1.37E−02
F
21
LNU128_H17
0.74
1.40E−02
F
19







LNU45_H300
0.70
2.32E−02
A
12





Table 52. “Correl. Set ID”—correlation set ID according to the correlated parameters Table above.






Correlation of early vigor traits across collection of Tomato ecotypes under Low nitrogen, 300 mM NaCl, and normal growth conditions—Ten tomato hybrids were grown in 3 repetitive plots, each containing 17 plants, at a net house under semi-hydroponics conditions. Briefly, the growing protocol was as follows: Tomato seeds were sown in trays filled with a mix of vermiculite and peat in a 1:1 ratio. Following germination, the trays were transferred to the high salinity solution (300 mM NaCl in addition to the Full Hoagland solution), low nitrogen solution (the amount of total nitrogen was reduced in a 90% from the full Hoagland solution, final amount of 0.8 mM N) or at Normal growth solution (Full Hoagland containing 8 mM N solution, at 28±2° C.). Plants were grown at 28±2° C.
Full Hoagland solution consists of: KNO3—0.808 grams/liter, MgSO4—0.12 grams/liter, KH2PO4—0.172 grams/liter and 0.01% (volume/volume) of ‘Super coratin’ micro elements (Iron-EDDHA [ethylenediamine-N,N′-bis(2-hydroxyphenylacetic acid)]-40.5 grams/liter; Mn—20.2 grams/liter; Zn 10.1 grams/liter; Co 1.5 grams/liter; and Mo 1.1 grams/liter), solution's pH should be 6.5-6.8].
Analyzed Sorghum tissues—All 10 selected Tomato varieties were sample per each treatment. Three tissues [leaves, meristems and flowers] were sampled and RNA was extracted as described above. For convenience, each micro-array expression information tissue type has received a Set ID as summarized in Table 53 below.







TABLE 53







Tomato transcriptom experimental sets










Expression Set
Set ID







Leaves at 300 mM NaCl
A



Leaves at Normal conditions
B



Leaves at Low Nitrogen conditions
C



Roots at 100 mM NaCl
D



Roots at Normal conditions
E



Roots at Low Nitrogen conditions
F







Table 53. Provided are the tomato transcriptom experimental sets.






Tomato vigor related parameters—following 5 weeks of growing, plant were harvested and analyzed for Leaf number, plant height, and Plant weight. Next, analyzed data was saved to text files and processed using the JMP statistical analysis software (SAS institute). Data parameters collected are summarize in Table 54, hereinbelow.







TABLE 54







Tomato correlated parameters (vectors)










Correlation Set
Correlation ID







Plant height_NUE [cm]
1



SPAD NUE [SPAD unit]
2



leaf No NUE [number]
3



leaf No Normal [number]
4



Plant height Normal [cm]
5



SPAD Normal [SPAD unit]
6



Leaf No NaCl [number]
7



Plant height NaCl [cm]
8



Plant biomass_NaCl [gr]
9







Table 54. Provided are the tomato correlated parameters.






Experimental Results
10 different Tomato varieties were grown and characterized for 3 parameters as described above. The average for each of the measured parameter was calculated using the JMP software and values are summarized in Tables 55 below. Subsequent correlation analysis was conducted (Tables 56 and 57). Follow, results were integrated to the database.







TABLE 55







Measured parameters in Tomato accessions under normal, salinity and


low nitrogen conditions
















Seed ID
1
2
3
4
5
6
7
8
9



















1139
36.8
34.6
5.56
6.56
45.3
34.3
3.56
5.6
0.36


2078
39.9
24.9
6.22
6.89
47.8
25.3
3.94
6.46
0.44


2958
34.4
28.6
7.22
7.33
40.8
28.1
5
8.47
0.26


5077
47
31.6
6.78
6.22
55.3
31.4
4
8.56
0.71


5080
46.4
29.7
5.56
6.33
56.2
30.2
3.56
8.87
0.46


5084
45.4
31.8
6.56
6.44
48.7
32.4
4.39
7.56
0.54


5085
47.7
30.3
5.11
5.89
55.8
32.6
3.17
8.64
0.66


5088
39.3
30.3
5.89
5.56
37.4
28.8
3.72
5.57
0.4


5089
41.8
31.3
5.56
6.11
49.6
30.9
4
5.82
0.52


5092
41
28.8
6.33
5.67
46.3
29
4.28
9.36
0.45
















TABLE 56







Correlation between the expression level of selected LNU genes


of some embodiments of the invention in various tissues and


the phenotypic performance under low nitrogen, normal or


salinity stress conditions across Tomato accessions











Gene Name
R
P value
Exp. set
Correl. Set ID





LNU245
0.81
1.41E−02
E
6





Table 56. “Correl. Set ID”—correlation set ID according to the correlated parameters Table above.













TABLE 57







Correlation between the expression level of selected LNU homologous genes of some


embodiments of the invention in various tissues and the phenotypic performance


under low nitrogen, normal or salinity stress conditions across Tomato accessions




















Correl.




Correl.


Gene

P
Exp.
Set
Gene

P
Exp.
Set


Name
R
value
Set
ID
Name
R
value
Set
ID





LNU128_H17
0.73
4.14E−02
B
4
LNU46_H78
0.88
4.04E−03
C
3


LNU128_H17
0.71
4.86E−02
B
4
LNU7_H146
0.77
2.68E−02
F
3


LNU128_H17
0.72
4.35E−02
E
4
LNU74_H204
0.71
4.70E−02
F
3


LNU74_H203
0.71
4.77E−02
C
3
LNU74_H205
0.75
1.20E−02
D
9


LNU46_H78
0.88
3.94E−03
C
3
LNU7_H146
0.74
1.38E−02
D
8





Table 57. “Correl. Set ID”—correlation set ID according to the correlated parameters Table above.






Example 11
Gene Cloning and Generation of Binary Vectors for Plant Expression
To validate their role in improving yield, selected genes were over-expressed in plants, as follows.
Cloning Strategy
Selected genes from those presented in Examples 1-10 hereinabove were cloned into binary vectors for the generation of transgenic plants. For cloning, the full-length open reading frames (ORFs) were identified. EST clusters and in some cases mRNA sequences were analyzed to identify the entire open reading frame by comparing the results of several translation algorithms to known proteins from other plant species.
In order to clone the full-length cDNAs, reverse transcription (RT) followed by polymerase chain reaction (PCR; RT-PCR) was performed on total RNA extracted from leaves, roots or other plant tissues, growing under normal/limiting or stress conditions. Total RNA extraction, production of cDNA and PCR amplification is performed using standard protocols described elsewhere (Sambrook J., E. F. Fritsch, and T. Maniatis. 1989. Molecular Cloning. A Laboratory Manual, 2nd Ed. Cold Spring Harbor Laboratory Press, New York.) which are well known to those skilled in the art. PCR products are purified using PCR purification kit (Qiagen)
Usually, 2 sets of primers were prepared for the amplification of each gene, via nested PCR (if required). Both sets of primers were used for amplification on cDNA. In case no product was obtained, a nested PCR reaction was performed. Nested PCR was performed by amplification of the gene using external primers and then using the produced PCR product as a template for a second PCR reaction, where the internal set of primers are used. Alternatively, one or two of the internal primers are used for gene amplification, both in the first and the second PCR reactions (meaning only 2-3 primers were designed for a gene). To facilitate further cloning of the cDNAs, an 8-12 bp extension was added to the 5′ of each internal primer. The primer extension includes an endonuclease restriction site. The restriction sites were selected using two parameters: (a) the restriction site does not exist in the cDNA sequence; and (b) the restriction sites in the forward and reverse primers were designed such that the digested cDNA was inserted in the sense direction into the binary vector utilized for transformation.
PCR products were digested with the restriction endonucleases (New England BioLabs Inc) according to the sites designed in the primers. Each digested PCR product was inserted into a high copy vector pBlue-script KS plasmid vector [pBlue-script KS plasmid vector, Hypertext Transfer Protocol://World Wide Web (dot) stratagene (dot) com/manuals/212205 (dot) pdf), or into plasmids originated from this vector. In case of the high copy vector originated from pBlue-script KS plasmid vector (pGXN or pGXNa), the PCR product was inserted in the high copy plasmid upstream to the NOS terminator (SEQ ID NO:4683) originated from pBI 101.3 binary vector (GenBank Accession No. U12640, nucleotides 4356 to 4693) and downstream to the 35S promoter (SEQ ID NO:4685). The digested products and the linearized plasmid vector are ligated using T4 DNA ligase enzyme (Roche, Switzerland). In some cases PCR products were cloned without digestion into pCR-Blunt II-TOPO vector (Invitrogen).
Sequencing of the inserted genes was performed, using the ABI 377 sequencer (Applied Biosystems). In some cases, after confirming the sequences of the cloned genes, the cloned cDNA accompanied/or not with the NOS terminator was introduced into a modified pGI binary vector containing the At6669 promoter or the RootP promoter via digestion with appropriate restriction endonucleases. In any case the insert was followed by single copy of the NOS terminator (SEQ ID NO:5683).
Several DNA sequences of the selected genes are synthesized by GeneArt [Hypertext Transfer Protocol://World Wide Web (dot) geneart (dot) com/]. Synthetic DNA was designed in silico. Suitable restriction enzymes sites are added to the cloned sequences at the 5′ end and at the 3′ end to enable later cloning into the desired binary vector.
The pPI plasmid vector was constructed by inserting a synthetic poly-(A) signal sequence, originating from pGL3 basic plasmid vector (Promega, GenBank Accession No. U47295; nucleotides 4658-4811) into the HindIII restriction site of the binary vector pBI101.3 (Clontech, GenBank Accession No. U12640). pGI (FIG. 1) was similar to pPI, but the original gene in the backbone was GUS-Intron and not GUS.
The modified pGI vector (pQFN or pQNa_RP) was a modified version of the pGI vector in which the cassette was inverted between the left and right borders so the gene and its corresponding promoter are close to the right border and the NPTII gene was close to the left border.
At6669, the new Arabidopsis thaliana promoter sequence (SEQ ID NO:4687) or the Root P promoter sequence (SEQ ID NO:4688) was inserted in the modified pGI binary vector, upstream to the cloned genes, followed by DNA ligation and binary plasmid extraction from positive E. coli colonies, as described above. Colonies were analyzed by PCR using the primers covering the insert which were designed to span the introduced promoter and gene. Positive plasmids were identified, isolated and sequenced.
In case genomic DNA was cloned, the genes were amplified by direct PCR on genomic DNA extracted from leaf tissue using the DNAeasy kit (Qiagen Cat. No. 69104).
Table 58 below provides primers used for cloning of selected genes.







TABLE 58







The PCR primers used for cloning the genes of some


 embodiments of the invention into high copy vectors










Restriction




Enzymes



Gene
used for



Name
cloning
Primers used for amplification





LNU1
BamHI, KpnI
LNU1_EF_BamHI(SEQ IN NO: 4689)


(6669)

AAAGGATCCAAATCTCAGCTTCACCATTCG




LNU1_ER2_KpnI(SEQ IN NO: 4690)




TTTGGTACCTTTCTTCGAGTCTGGTCTCATTATC


 


LNU1
BamHI, KpnI
LNU1_EF_BamHI(SEQ IN NO: 4689)


(Root_P_F)

AAAGGATCCAAATCTCAGCTTCACCATTCG




LNU1_ER2_KpnI(SEQ IN NO: 4690)




TTTGGTACCTTTCTTCGAGTCTGGTCTCATTATC


 


LNU10

LNU10_NF_XhoI(SEQ IN NO: 4691)




AAACTCGAGCATTAAATTCGATCGAGGCTTTC




LNU10_EF_XhoI(SEQ IN NO: 4692)




AAACTCGAGCAACTCGGTTGCATTAAATTCG




LNU10_NR_EcoRV(SEQ IN NO: 4693)




AAAGATATCAAATACAGCTTGATGGTCGGTG




LNU10_ER_EcoRV(SEQ IN NO: 4694)




AAAGATATCTGATATGGACATGTTTGCAAGG


 


LNU100
BamHI, XhoI
LNU100_EF_BamHI(SEQ IN NO: 4695)




AAAGGATCCTAAAGCACTTCACCTTTGCTCC




LNU100_ER_XhoI(SEQ IN NO: 4696)




AAACTCGAGATACAAATATAACAAGCCAATCATGC


 


LNU101
BamHI, XhoI
LNU101_NF_BamHI(SEQ IN NO: 4697)




AAAGGATCCTATATGTTACACGATGCCGTCC




LNU101_NF_BamHI(SEQ IN NO: 4697)




AAAGGATCCTATATGTTACACGATGCCGTCC




LNU101_NR_XhoI(SEQ IN NO: 4698)




AAACTCGAGCGACTCAAATTCATCTTAACAAGC




LNU101_NR_XhoI(SEQ IN NO: 4698)




AAACTCGAGCGACTCAAATTCATCTTAACAAGC


 


LNU104
SalI, XbaI
LNU104_ER_XbaI(SEQ IN NO: 4699)




AAATCTAGAAAGCAAATTTCGTTTGCAACTC




LYD104_EF_BamI(SEQ IN NO: 4700)




AAAGGATCCTCCCAATAAACCCTAATTCCTTG




LNU104_EF_SalI(SEQ IN NO: 4701)




AAAGTCGACTCCATTGGCCGTAGTAGCAG


 


LNU105
BamHI, XhoI
LNU105_EF_BamHI(SEQ IN NO: 4702)




AAAGGATCCCTTCTTCCAGCTCCGGTTC




LNU105_ER_XhoI(SEQ IN NO: 4703)




AAACTCGAGACTCGTCATCTATGCACTCGAC


 


LNU106 
SalI, XbaI
LNU106_NF_SalI(SEQ IN NO: 4704)




AAAGTCGACACGTCTTGGTTTGTCGGTTAAG




LNU106_EF_SalI(SEQ IN NO: 4705)




AAAGTCGACGTCTCTTCCTCTCCACAAGCAC




LNU106_NR_XbaI(SEQ IN NO: 4706)




AAATCTAGATACCAGCGATTCATATTGGAGG




LNU106_ER_XbaI(SEQ IN NO: 4707)




AAATCTAGACGATCTCATAAACGGATTCGAG


 


LNU107
BamHI, XhoI
LNU107_NF_BamHI(SEQ IN NO: 4708)




AAAGGATCCCCATTTCCATATTCCGTCTGTC




LNU107_EF_BamHI(SEQ IN NO: 4709)




AAAGGATCCCTTCTTCTGCGAATTTCCTCTG




LNU107_R_XhoI(SEQ IN NO: 4710)




AAACTCGAGCTACAAGCAGATCAACTCAGGGAG




LNU107_R_XhoI(SEQ IN NO: 4710)




AAACTCGAGCTACAAGCAGATCAACTCAGGGAG


 


LNU109
XhoI, EcoRV
LNU109_EF_XhoI(SEQ IN NO: 4711)




AAACTCGAGAGCTCACACCGATCCAGTAATC




LNU109_ER_EcoRV(SEQ IN NO: 4712)




AAAGATATCCTTTATGGGAGAGGACATGCAC


LNU110
BamHI, XhoI
LYD110_EF_BamHI(SEQ IN NO: 4713)




AAAGGATCCTAACCTCATAGTGTCGACATGG




LYD110_ER_KpnI(SEQ IN NO: 4714)




AAAGGTACCTTCACCAACTTATACGAACCAC


 


LNU113
BamHI, XhoI
LNU113_EF_BamHI(SEQ IN NO: 4715)




AAAGGATCCGAGCAAGATCAATCCCTCTGC




LNU113_ER_XhoI(SEQ IN NO: 4716)




AAACTCGAGGAAGAAAGCCATCACAAGCATC


 


LNU114
SalI, XbaI
LNU114_EF_SalI(SEQ IN NO: 4717)




AAAGTCGACTGGTAGTGAACCGTGAACACAC




LNU114_ER_XbaI(SEQ IN NO: 4718)




AAATCTAGAACAGGAGCTCAGAAGCTTCAAC


 


LNU115
SmaI, KpnI
LNU115_EF2_SmaI(SEQ IN NO: 4719)




AAACCCGGGGTGTCCCTGTACCAGATCCAC




LNU115_ER2_KpnI(SEQ IN NO: 4720)




AAAGGTACCCTCCAAAATTATCATTAACACCG


 


LNU116
BamHI, KpnI
LNU116_NF_BamHI(SEQ IN NO: 4721)




AAAGGATCCTTGATCCATTCATCTTTGTTGG




LNU116_EF_BamHI(SEQ IN NO: 4722)




AAAGGATCCGCTTGTGTTTCTCGAAATTGTG




LNU116_R_KpnI(SEQ IN NO: 4723)




AAAGGTACCAAGAATGGCCTAAGCTACCGAC




LNU116_R_KpnI(SEQ IN NO: 4723)




AAAGGTACCAAGAATGGCCTAAGCTACCGAC


 


LNU117
BamHI, XhoI
LNU117_NF_BamHI(SEQ IN NO: 4724)




AAAGGATCCGCATGAGCATGACTCCTCAC




LNU117_EF_BamHI(SEQ IN NO: 4725)




AAAGGATCCGAGACCAGACGCAGAAGATGTC




LNU117_NR_XhoI(SEQ IN NO: 4726)




AAACTCGAGACTATTTGCCGTGCATAACGAC




LNU117_ER_XhoI(SEQ IN NO: 4727)




AAACTCGAGACAAACAACCGCGTAAGAAGAG


 


LNU118
BamHI, XhoI
LNU118_NF_BamHI(SEQ IN NO: 4728)


(6669)

AAAGGATCCCTAATTCAGCTAAGGATTTGGAGG




LNU118_NR_XhoI(SEQ IN NO: 4729)




AAACTCGAGCGCTGACTCGATCGTTGAC


 


LNU118
BamHI, XhoI
LNU118_NF_BamHI(SEQ IN NO: 4728)


(Root_P_F)

AAAGGATCCCTAATTCAGCTAAGGATTTGGAGG




LNU118_NR_XhoI(SEQ IN NO: 4729)




AAACTCGAGCGCTGACTCGATCGTTGAC


 


LNU119
BamHI, XhoI
LNU119_NF_BamHI(SEQ IN NO: 4730)




AAAGGATCCTTGCTACCCACCACGAGAG




LNU119_EF_BamHI(SEQ IN NO: 4731)




AAAGGATCCATATACGAGCCTTTGCTACCCAC




LNU119_NR_XhoI(SEQ IN NO: 4732)




AAACTCGAGTGCCAACTGTCTGAGATCTTTC




LNU119_ER_XhoI(SEQ IN NO: 4733)




AAACTCGAGATTGTGTCTTTGAGCTGCCAAC


 


LNU12 
BamHI, KpnI
LNU12_NF_BamHI(SEQ IN NO: 4734)




AAAGGATCCCTAGCGAACTACGTGTGCTCC




LNU12_EF_BamHI(SEQ IN NO: 4735)




AAAGGATCCGCTCTTCACACGGCTAACG




LNU12_NR_KpnI(SEQ IN NO: 4736)




AAAGGTACCGTTTCCACGCAAGGAAGAATC




LNU12_ER_KpnI(SEQ IN NO: 4737)




AAAGGTACCGTTCGATTCGGCTCTGTTTC


 


LNU120
SalI, XbaI
LNU120_NF_SalI(SEQ IN NO: 4738)




AAAGTCGACGTCATCACACATTGGCAGC




LNU120_EF_SalI(SEQ IN NO: 4739)




AAAGTCGACATCAGTCATCACACATTGGCAG




LNU120_NR_XbaI(SEQ IN NO: 4740)




AAATCTAGAACATGGTTGATCTTGAGCTGTG




LNU120_ER_XbaI(SEQ IN NO: 4741)




AAATCTAGACGACATGGTTGATCTTGAGC


 


LNU121 
XhoI, StuI
LNU121_F_XhoI(SEQ IN NO: 4742)




AAACTCGAGAAAAACGCGCAATCCCG




LNU121_ER_StuI(SEQ IN NO: 4743)




TTTAGGCCTGGGTTTGGTCATGTACAGTCAC


 


LNU122

LNU122_NF_BamHI(SEQ IN NO: 4744)




AAAGGATCCAACGAATAGCCAAGCTCAGTTC




LNU122_NR_KpnI(SEQ IN NO: 4745)




AAAGGTACCATTTGATTATTTGTGGTGTACAATGC


 


LNU123
BamHI, XhoI
LNU123_F_BamHI(SEQ IN NO: 4746)




AAAGGATCCGATCCGAAAGGATCTCCACC




LNU123_F_BamHI(SEQ IN NO: 4746)




AAAGGATCCGATCCGAAAGGATCTCCACC




LNU123_NR_XhoI(SEQ IN NO: 4747)




AAACTCGAGATGCTTCCTCATTGTTTGATCC




LNU123_ER_XhoI(SEQ IN NO: 4748)




AAACTCGAGATACCAATTCTAACCGTGGTCG


 


LNU124
BamHI, KpnI
LNU124_NF_BamHI(SEQ IN NO: 4749)




AAAGGATCCAATTAATTCGAAAGAGCGGTCAC




LNU124_EF_BamHI(SEQ IN NO: 4750)




AAAGGATCCATTCACTACATGCACAAGCACG




LNU124_NR_KpnI(SEQ IN NO: 4751)




AAAGGTACCCTAGATCCAATGGAGAGACAGAGC




LNU124_ER_KpnI(SEQ IN NO: 4752)




AAAGGTACCAAAGTCTCTGGAGTTGATGAAATTG


 


LNU125
SalI, SacI
LNU125_F2_Sal(SEQ IN NO: 4753)




TTTGTCGACTGACTTTAAAAATTTGAACGTGAA




LNU125_R2_Sac(SEQ IN NO: 4754)




TTTGAGCTCGTGGAAGGTTACACTGTTGTATTTC


 


LNU126
BamHI, XhoI
LNU126_F_BamHI(SEQ IN NO: 4755)




AATGGATCCCTATCACAAAGCCTAGAGTAAAATCG




LNU126_F_BamHI(SEQ IN NO: 4755)




AATGGATCCCTATCACAAAGCCTAGAGTAAAATCG




LNU126_NR_XhoI(SEQ IN NO: 4756)




AAACTCGAGGCAACACAAGGAACTGTACTATCTC




LNU126_ER_XhoI(SEQ IN NO: 4757)




TTTCTCGAGTCAGCGGTTACTTTGTCGTTAC


 


LNU128
BamHI, XhoI
LNU128_F_BamHI(SEQ IN NO: 4758)




AAAGGATCCAGGCGAAGAAGAGAGAGGAATG




LNU128_F_BamHI(SEQ IN NO: 4758)




AAAGGATCCAGGCGAAGAAGAGAGAGGAATG




LNU128_NR_XhoI(SEQ IN NO: 4759)




AAACTCGAGCTATAAGGCACAGGTCCAATTCAAG




LNU128_ER_XhoI(SEQ IN NO: 4760)




AAACTCGAGTGATTCGATCATGTATTTCACATTG


 


LNU129
BamHI, XhoI
LNU129_NF_BamHI(SEQ IN NO: 4761)




AAAGGATCCGTTTGTCTCGCATGAGGATTTG




LNU129_NF_BamHI(SEQ IN NO: 4761)




AAAGGATCCGTTTGTCTCGCATGAGGATTTG




LNU129_NR_XhoI(SEQ IN NO: 4762)




AAACTCGAGTGAAATTTCTCTGTTGGATTGATG




LNU129_NR_XhoI(SEQ IN NO: 4762)




AAACTCGAGTGAAATTTCTCTGTTGGATTGATG


 


LNU130 
SalI, XbaI
LNU130_F_SalI(SEQ IN NO: 4763)




AAAGTCGACCTGAAAGACGAAGAAGAGAAACG




LNU130_F_SalI(SEQ IN NO: 4763)




AAAGTCGACCTGAAAGACGAAGAAGAGAAACG




LNU130_NR_XbaI(SEQ IN NO: 4764)




AAATCTAGAATGAACAACGGTTTCAATGGAC




LNU130_ER_XbaI(SEQ IN NO: 4765)




AAATCTAGAATCGGTGTAAGTGAACACGATG


 


LNU131
BamHI, XhoI
LNU131_EF_BamHI(SEQ IN NO: 4766)




AAAGGATCCCTTCTTCTTCTTCGATTTAGCACAG




LNU131_ER_XhoI(SEQ IN NO: 4767)




AAACTCGAGCATTGTTGGCTGTATATTTCATCAC


 


LNU132 
SalI, XbaI
LNU132_F_SalI(SEQ IN NO: 4768)




AAAGTCGACTCTTTCTGCAGAGATTATGGAGG




LNU132_ER_XbaI(SEQ IN NO: 4769)




AAATCTAGAAATCGCAGAGAAGCAAACAGAC


 


LNU133 
SalI, XbaI
LNU133_EF_SalI(SEQ IN NO: 4770)




AAAGTCGACAAATTTCCAGAGAAGTCGTTCATC




LNU133_ER_XbaI(SEQ IN NO: 4771)




AAATCTAGAATTACAGCATCAAACAGCCAGC


 


LNU134
BamHI, XhoI
LNU134_NF_BamHI(SEQ IN NO: 4772)




AAAGGATCCAGGTTTCTTTCGATTCGTTGAG




LNU134_EF_BamHI(SEQ IN NO: 4773)




AAAGGATCCGTTATTCTCAATCCTTCCTTCATCC




LNU134_NR_XhoI(SEQ IN NO: 4774)




AAACTCGAGCTACCTGTACTTTGGGAATAAGCAGAG




LNU134_ER_XhoI(SEQ IN NO: 4775)




AAACTCGAGGAGTTCTTTCACATCATGGACG


 


LNU135
BamHI, XhoI
LNU135_EF_BamHI(SEQ IN NO: 4776)




AAAGGATCCAGCCGTTTCTTTCCGATTC




LNU135_ER_XhoI(SEQ IN NO: 4777)




AAACTCGAGACGAGAAATATGATCACTGGAAATC


 


LNU136
BamHI, KpnI
LNU136_EF_BamHI(SEQ IN NO: 4778)




AAAGGATCCTCGGAGACTGAATGATATTGTTTC




LNU136_ER_KpnI(SEQ IN NO: 4779)




AAAGGTACCTTCAAAGAATGTGTCTTGTGTGTG


 


LNU138
EcoRV, SalI
LNU138_EF_SalI(SEQ IN NO: 4780)




AAAGTCGACATAAAGATCGTCCACAAGGAGG




LNU138_ER_EcoRV(SEQ IN NO: 4781)




AAAGATATCCAATCAGCATACAAAGGCACAC


 


LNU14 
EcoRV, XhoI
LNU14_EF_XhoI(SEQ IN NO: 4782)




AAACTCGAGTTCTTAGGGACCATTCCTCCTC




LNU14_R_EcoRV(SEQ IN NO: 4783)




AAAGATATCCTATGGTTTCATCAAATAAGACACACA


 


LNU140
SalI, XbaI
LNU140_NF_SalI(SEQ IN NO: 4784)




AAAGTCGACGCTGTTTCTTCCCGATCTTTG




LNU140_EF_SalI(SEQ IN NO: 4785)




AAAGTCGACGTTAACCTCTCCTCGTTCTCGTC




LNU140_NR_XbaI(SEQ IN NO: 4786)




AAATCTAGACTATCGAGAGGATTTACAATGGCAG




LNU140_ER_XbaI(SEQ IN NO: 4787)




AAATCTAGACGAATCATGAGACAAACAAACC


 


LNU141
BamHI, XhoI
LNU141_NF_BamHI(SEQ IN NO: 4788)




AAAGGATCCCGTCTCACTTCATCCCATCC




LNU141_EF_BamHI(SEQ IN NO: 4789)




AAAGGATCCCTTCCGACCTCACGAAAGC




LNU141_NR_XhoI(SEQ IN NO: 4790)




AAACTCGAGACGGCTTAAGATTTGTACAGCAC




LNU141_ER_XhoI(SEQ IN NO: 4791)




AAACTCGAGCACCATCTATGCACGTCAACTG


 


LNU143
BamHI, XhoI
LNU143_EF_BamHI(SEQ IN NO: 4792)




AATGGATCCCAAGCCTACGGTGTTCATGAC




LNU143_ER_XhoI(SEQ IN NO: 4793)




AAACTCGAGCATCTATAGGGAACACGAATGAGC


 


LNU147
BamHI, XhoI
LNU147_EF_BamHI(SEQ IN NO: 4794)




AAAGGATCCCTCTTCTTGAACATGACAAAGACC




LNU147_ER_XhoI(SEQ IN NO: 4795)




AAACTCGAGAGGATTCACGCCATACAGTTTAG


 


LNU148
BamHI, XhoI
LNU148_F_BamHI(SEQ IN NO: 4796)




TTTGGATCCGTCTATTGCATTGAGTTGAAATCAC




LNU148_F_BamHI(SEQ IN NO: 4796)




TTTGGATCCGTCTATTGCATTGAGTTGAAATCAC




LNU148_NR_XhoI(SEQ IN NO: 4797)




AATCTCGAGTCAATCAAATTGTGTATTCAAATGTATA




TAC




LNU148_ER_XhoI(SEQ IN NO: 4798)




TATCTCGAGTCCCAAAATTCAAGCTAACAGTC


 


LNU149 
BamHI, XhoI
LNU149_NF_BamHI(SEQ IN NO: 4799)




AAAGGATCCATTCAGAATTGGAGAGGGAAGG




LNU149_EF_BamHI(SEQ IN NO: 4800)




AAAGGATCCCTAGCTCAGGCCATTGAAGAAC




LNU149_NR_XhoI(SEQ IN NO: 4801)




AAACTCGAGCCGGGTTTACTCAGTATGAAGC




LNU149_ER_XhoI(SEQ IN NO: 4802)




AAACTCGAGGAGCTTACACGAACGTTTCTCC


 


LNU15
SalI, XbaI
LNU15_NF_SalI(SEQ IN NO: 4803)




AAAGTCGACCTTCTCTCCGCAACACTGAAAC




LNU15_EF_SalI(SEQ IN NO: 4804)




AAAGTCGACACCAAACTTTGCCTTTCTCTCTC




LNU15_NR_XbaI(SEQ IN NO: 4805)




AAATCTAGAGGTTCCTTATTATTTCACACCCAAG




LNU15_ER_XbaI(SEQ IN NO: 4806)




AAATCTAGAAGAACATCAAATCTAGTCGCAGTG


 


LNU150
SalI, XbaI
LNU150_EF_SalI(SEQ IN NO: 4807)




AAAGTCGACCACCGCTTTGTGGAAACAG




LNU150_ER_XbaI(SEQ IN NO: 4808)




AAATCTAGAGGCAGTTGCTTCCATTATTGC


 


LNU153 
BamHI, XhoI
LNU153_EF_BamHI(SEQ IN NO: 4809)




AAAGGATCCTGTCCACTTTGGTTCCTTCTTC




LNU153_ER_XhoI(SEQ IN NO: 4810)




AAACTCGAGTGCTCTACAATCATCACCATCC


 


LNU154 
XhoI, EcoRV
LNU154_EF_XhoI(SEQ IN NO: 4811)




AAACTCGAGCAAAGAAGAAACTAGTTGTAGGCAGC




LNU154_ER_EcoRV(SEQ IN NO: 4812)




AAAGATATCTGGTAATGATACAAGCTCAAGCAAC


 


LNU155
SalI, XbaI
LNU155_EF_SalI(SEQ IN NO: 4813)




AAAGTCGACTTCTTTACCCATTATTGCACTCAC




LNU155_ER_XbaI(SEQ IN NO: 4814)




AAATCTAGACAGTTTCCACAAATTCTCAATTACG


 


LNU157
SalI, XbaI
LNU157_EF_SalI(SEQ IN NO: 4815)




AAAGTCGACCAAAGTTCACACACAGAAGAATCAG




LNU157_R_XbaI(SEQ IN NO: 4816)




ATTTCTAGATCTTTCAATTACTTCAATTAGCCTCC


 


LNU158 
BamHI, XhoI
LNU158_NF_BamHI(SEQ IN NO: 4817)




TTTGGATCCGAAAGTTCCTCACAATCATTTGTC




LNU158_EF_BamHI(SEQ IN NO: 4818)




TTTGGATCCGCACCCTTTGGTAGATTCTCG




LNU158_NR_XhoI(SEQ IN NO: 4819)




ATTCTCGAGTCAAAGATTGAAGGTATCATATGCTGTTCA




LNU158_ER_XhoI(SEQ IN NO: 4820)




TTTCTCGAGTGGTTTGTGGGTAATCTTCTGC


 


LNU161
SalI, XbaI
LNU161_NF_SalI(SEQ IN NO: 4821)




AAAGTCGACACTTTCTCTCTTCGGGTTCTCG




LNU161_NF_SalI(SEQ IN NO: 4821)




AAAGTCGACACTTTCTCTCTTCGGGTTCTCG




LNU161_NR_XbaI(SEQ IN NO: 4822)




AAATCTAGAATCGCTTATTTCCGACCACAC




LNU161_NR_XbaI(SEQ IN NO: 4822)




AAATCTAGAATCGCTTATTTCCGACCACAC


 


LNU168 
XhoI, XbaI
LNU168_EF_XhoI(SEQ IN NO: 4823)




AAACTCGAGCTTCCCTTCCATACTTGCTTCC




LNU168_ER_SacI(SEQ IN NO: 4824)




AAAGAGCTCTGTCACTCAAAGGTAGCTGAGG


 


LNU17 
BamHI, KpnI
LNU17_EF_BamHI(SEQ IN NO: 4825)




AAAGGATCCTGCCATAAGCTTCCATCCTATC




LNU17_ER_KpnI(SEQ IN NO: 4826)




AAAGGTACCTGTGCTTCCTAAGCTTTCAACTC


 


LNU170 
SalI, SacI
LNU170_NF_SalI(SEQ IN NO: 4827)




TTAGTCGACATGTAATGGCTACTTCTTCCTCTTCTTG




LNU170_EF_SalI(SEQ IN NO: 4828)




TTAGTCGACATGTTCTTCACTGTAATGTAATGGCTAC




LNU170_NR_SacI(SEQ IN NO: 4829)




ATAGAGCTCCAATGCATGAATTCCTCGTG




LNU170_ER_SacI(SEQ IN NO: 4830)




TAAGAGCTCCTGATTACGTTAGGTAGGTGTGTGTATC


 


LNU171
SalI, XbaI
LNU171_NF_SalI(SEQ IN NO: 4831)




AAAGTCGACCTAGCAGAGGCAGAGCCTACAG




LNU171_F2_Sal(SEQ IN NO: 4832)




AATGTCGACCGATCAACTAGGCAACTAGCA




LNU171_NR_XbaI(SEQ IN NO: 4833)




AAATCTAGACTACTAAGCATGAACACCTGGTGAG




LNU171_R2_Xba(SEQ IN NO: 4834)




AAATCTAGAGAGAAATCTGTTCCTGGACACA


 


LNU172
XhoI, EcoRV
LNU172_EF_XhoI(SEQ IN NO: 4835)




AAACTCGAGCGAGCACTTCTCTAGCTCATGC




LNU172_ER_EcoRV(SEQ IN NO: 4836)




AAAGATATCGAACCCAATCCGAATTAATTGAC


 


LNU173 
SalI, XbaI
LNU173_NF_SalI(SEQ IN NO: 4837)




AAAGTCGACACATCGTACGTCCGTTCCAG




LNU173_NF_SalI(SEQ IN NO: 4837)




AAAGTCGACACATCGTACGTCCGTTCCAG




LNU173_NR_XbaI(SEQ IN NO: 4838)




AAATCTAGAAACGGAACATTTGAATGACTGC




LNU173_NR_XbaI(SEQ IN NO: 4838)




AAATCTAGAAACGGAACATTTGAATGACTGC


 


LNU175
BamHI, XhoI
LNU175_EF_BamHI(SEQ IN NO: 4839)




AAAGGATCCTCTCTCATCTGCCTACGGTTG




LNU175_ER_XhoI(SEQ IN NO: 4840)




AAACTCGAGAATCATGCCTCTTGTCTTGGTG


 


LNU176 
SalI, XbaI
LNU176_NF_SalI(SEQ IN NO: 4841)




AAAGTCGACCTCTCTCAAGGTCTCACCAACC




LNU176_NR_XbaI(SEQ IN NO: 4842)




AAATCTAGATGCATTACACACAGTAACATCATCAG


 


LNU177
SalI, XbaI
LNU177_NF_SalI(SEQ IN NO: 4843)




AAAGTCGACGATCATCATCAGACAATGGCAG




LNU177_EF_SalI(SEQ IN NO: 4844)




AAAGTCGACAATTTCCATTGGTCCTCCTCTC




LNU177_R_XbaI(SEQ IN NO: 4845)




AAATCTAGAACATTTGAATCCCAAAGATGATTT




LNU177_R_XbaI(SEQ IN NO: 4845)




AAATCTAGAACATTTGAATCCCAAAGATGATTT


 


LNU178 
BamHI, XhoI
LNU178_EF_BamHI(SEQ IN NO: 4846)




AAAGGATCCTCTCTCTTGTTCTGAATTCGTGG




LNU178_ER_XhoI(SEQ IN NO: 4847)




AAACTCGAGGACAGAGAGAAGCTATGACCAACTG


 


LNU179
BamHI, XhoI
LNU179_F_BamHI(SEQ IN NO: 4848)




AAAGGATCCGAGATAGAGAGAGAGATAATGGGCA




LNU179_ER_XhoI(SEQ IN NO: 4849)




AAACTCGAGTGCACACTTAAATCAACAAGCA


 


LNU180
BamHI, XhoI
LNU180_NF_BamHI(SEQ IN NO: 4850)




AAAGGATCCGTTCTATGTTCCTGAAATGGGATT




LNU180_EF_BamHI(SEQ IN NO: 4851)




AAAGGATCCGAAACAAGCTCCATATCAATAATCAA




LNU180_NR_XhoI(SEQ IN NO: 4852)




AAACTCGAGGAACGGAAGAAATAACCAACAAA




LNU180_ER_XhoI(SEQ IN NO: 4853)




AAACTCGAGATGGTTTGAAGAACGGAAGAAA


 


LNU181 
BamHI, KpnI
LNU181_NF_BamHI(SEQ IN NO: 4854)




AAAGGATCCGATTTCTTCGTCAGTTGCGTTT




LNU181_EF_BamHI(SEQ IN NO: 4855)




AAAGGATCCCGGTCCTAAACCCTACTCAACA




LNU181_NR_KpnI(SEQ IN NO: 4856)




AAAGGTACCAAATCTCATAGCTTATCATGCTCAAA




LNU181_ER_KpnI(SEQ IN NO: 4857)




AAAGGTACCTTCAGCCGTATCATCGTCTATTT


 


LNU182 
BamHI, XhoI
LNU182_NF_BamHI(SEQ IN NO: 4858)




AAAGGATCCCGTTGTGTTCCAACTCTCATTC




LNU182_EF_BamHI(SEQ IN NO: 4859)




AAAGGATCCGATTTGCGAGTCGTTGTGTTC




LNU182_NR_XhoI(SEQ IN NO: 4860)




AAACTCGAGGATCTTGAGGAACATGGAGACG




LNU182_ER_XhoI(SEQ IN NO: 4861)




AAACTCGAGGTGACTTTGGTTCCGATTTGAG


 


LNU183 
BamHI, XhoI
LNU183_F_BamHI(SEQ IN NO: 4862)




AACGGATCCAAGCTCTAGACTTTGTCTCTTTGTCC




LNU183_F_BamHI(SEQ IN NO: 4862)




AACGGATCCAAGCTCTAGACTTTGTCTCTTTGTCC




LNU183_NR_XhoI(SEQ IN NO: 4863)




AATCTCGAGTCACACCAATACAACCATAAATAACAC




LNU183_ER_XhoI(SEQ IN NO: 4864)




AATCTCGAGACTGCTGAAGTCAAAGCTAATTAGAAC


 


LNU184 
BamHI, XhoI
LNU184_NF_BamHI(SEQ IN NO: 4865)




AAAGGATCCCCTACCTAATCCACACCGATTC




LNU184_EF_BamHI(SEQ IN NO: 4866)




AAAGGATCCGAAGTGGAGAGAAGTGACCACC




LNU184_NR_XhoI(SEQ IN NO: 4867)




AAACTCGAGCAGCATGAGAAGAGATTTCGAG




LNU184_ER_XhoI(SEQ IN NO: 4868)




AAACTCGAGCAGCAACAACAAGAGATTTGTCC


 


LNU185 
BamHI, XhoI
LNU185_NF_BamHI(SEQ IN NO: 4869)




AAAGGATCCACAAACGGTGTGTAAGTGAAGAAG




LNU185_EF_BamHI(SEQ IN NO: 4870)




AAAGGATCCTCAGTCTGAAGACAAACGGTG




LNU185_NR_XhoI(SEQ IN NO: 4871)




AAACTCGAGCCGCAGAGGCTTTGTTAAATTC




LNU185_ER_XhoI(SEQ IN NO: 4872)




AAACTCGAGAAGGACATCATCAAAGCAGTACG


 


LNU186 
BamHI, XhoI
LNU186_EF_BamHI(SEQ IN NO: 4873)




AAAGGATCCATTGAGAGTCGCCACAGCTATC




LNU186_ER_XhoI(SEQ IN NO: 4874)




AAACTCGAGTGGCTTGATAAAGATTTGTGATTTC


 


LNU187 
XhoI, EcoRV
LNU187_NF_XhoI(SEQ IN NO: 4875)




AAACTCGAGCTCCTTCTTTACTTCGCTCACC




LNU187_EF_XhoI(SEQ IN NO: 4876)




AATCTCGAGTTTATCTCCTTCTTTACTTCGCTCAC




LNU187_NR_EcoRV(SEQ IN NO: 4877)




AATGATATCTTTGAAGCTAAACGATTTGACTAATTC




LNU187_ER_EcoRV(SEQ IN NO: 4878)




AATGATATCCCGCCACATTCATTTCAG


 


LNU188 
BamHI, XhoI
LNU188_NF_BamHI(SEQ IN NO: 4879)




AAAGGATCCAGAGCTTGCTCGGAGAGAGTG




LNU188_EF_BamHI(SEQ IN NO: 4880)




AAAGGATCCACAGAGAGATGCAGACCTGACC




LNU188_NR_XhoI(SEQ IN NO: 4881)




AAACTCGAGCCCTGATTCTCCTGTTGAGAAC




LNU188_ER_XhoI(SEQ IN NO: 4882)




AAACTCGAGTAAAGCTCGATTTCCCTGATTC


 


LNU189 
BamHI, XhoI
LNU189_EF_BamHI(SEQ IN NO: 4883)




AAAGGATCCAACAGACTGAATCATCAACGGAC




LNU189_ER_XhoI(SEQ IN NO: 4884)




AAACTCGAGCACGAGATGATAAGGGTTGGTC


 


LNU19
XhoI, SacI
LNU19_NF_XhoI(SEQ IN NO: 4885)




AAACTCGAGGCAGCTCGTGTGTGATTGAG




LNU19_NF_XhoI(SEQ IN NO: 4885)




AAACTCGAGGCAGCTCGTGTGTGATTGAG




LNU19_NR_SacI(SEQ IN NO: 4886)




AAAGAGCTCTCGTTTCCTACAAATGCAACAG




LNU19_NR_SacI(SEQ IN NO: 4886)




AAAGAGCTCTCGTTTCCTACAAATGCAACAG


 


LNU196
BamHI, KpnI
LNU196_NF_BamHI(SEQ IN NO: 4887)




AAAGGATCCGATCAATCCTTCTGCGTGTTC




LNU196_EF_BamHI(SEQ IN NO: 4888)




AAAGGATCCCCATATCACATCTCTGATCAATCC




LNU196_NR_KpnI(SEQ IN NO: 4889)




AAAGGTACCTACTGTGATCATAAGCTACGTGGAC




LNU196_ER_KpnI(SEQ IN NO: 4890)




AAAGGTACCGCACAACATGTGGTCAAATTATTC


 


LNU2 
BamHI, KpnI
LNU2_NF_BamHI(SEQ IN NO: 4891)




AAAGGATCCCTCCTCTTCCGCTCGAATTTAC




LNU2_EF_BamHI(SEQ IN NO: 4892)




AAAGGATCCACAACACCACAGCGCTCATAC




LNU2_NR_KpnI(SEQ IN NO: 4893)




AAAGGTACCAATCCTACCCACAACTGTCTGG




LNU2_ER_KpnI(SEQ IN NO: 4894)




AAAGGTACCTGAATTCCTCGCAAGAGTTACC


 


LNU20
SalI, XbaI
LNU20_EF_SalI(SEQ IN NO: 4895)




AAAGTCGACGAAGTGTTATTTGGAGGCAAGG




LNU20_ER_XbaI(SEQ IN NO: 4896)




AAATCTAGAACCATCAAATTTAGCCATGCAC


 


LNU200
XhoI, SacI
LNU200_NF_XhoI(SEQ IN NO: 4897)




AAACTCGAGATTTGGTCATAGTGTCGACATGG




LNU200_EF_XhoI(SEQ IN NO: 4898)




AAACTCGAGTGTGCTCCAAACTTGAAAGAAAG




LNU200_NR_SacI(SEQ IN NO: 4899)




AAAGAGCTCGACACGCAAATAGGACACACTG




LNU200_ER_SacI(SEQ IN NO: 4900)




AAAGAGCTCTTGAGACACGCAAATAGGACAC


 


LNU207
BamHI, XhoI
LNU207_NF_BamHI(SEQ IN NO: 4901)




AAAGGATCCCTTGGAGCTAGGAGACATCGTG




LNU207_EF_BamHI(SEQ IN NO: 4902)




AAAGGATCCTTATTTCCCTAAATCCTTGGAGC




LNU207_NR_XhoI(SEQ IN NO: 4903)




AAACTCGAGCTGACCACTTAACACTCTCACTCG




LNU207_ER_XhoI(SEQ IN NO: 4904)




AAACTCGAGAATCTCCCATACGACACTGACC


 


LNU210
BamHI, KpnI
LNU210_EF_BamHI(SEQ IN NO: 4905)




AAAGGATCCCGATCGATTGGTTTAAATCCTG




LNU210_ER_KpnI(SEQ IN NO: 4906)




AAAGGTACCTTACAATCACGACCACCTTGTAAC


 


LNU211 
SalI, XbaI
LNU211_NF_SalI(SEQ IN NO: 4907)




AAAGTCGACAACCTCCTTCTCAAACCGTAGG




LNU211_EF_SalI(SEQ IN NO: 4908)




AAAGTCGACAAAGGCCTAAGCTCAAGCAATC




LNU211_NR_XbaI(SEQ IN NO: 4909)




AAATCTAGAGGAAACCCTAATTTCCTTCTCC




LNU211_ER_XbaI(SEQ IN NO: 4910)




AAATCTAGAAAAGTTAGTGCTATTCGCGCTC


 


LNU212
SalI, XbaI
LNU212_F_SalI(SEQ IN NO: 4911)




TTTGTCGACCTACTCACATCATGGCTTTCTCC




LNU212_ER_XbaI(SEQ IN NO: 4912)




ATTTCTAGATTCACCGAATAATATATGCAAACG


 


LNU213
BamHI, XhoI
LNU213_EF_BamHI(SEQ IN NO: 4913)


(6669)

AAAGGATCCCAAATTAGGAGGAGCGAGAGC




LNU213_ER_XhoI(SEQ IN NO: 4914)




AAACTCGAGATGATCAGAAATTCTCAACCACG


 


LNU213
BamHI, XhoI
LNU213_EF_BamHI(SEQ IN NO: 4913)


(Root_P_F)

AAAGGATCCCAAATTAGGAGGAGCGAGAGC




LNU213_ER_XhoI(SEQ IN NO: 4914)




AAACTCGAGATGATCAGAAATTCTCAACCACG


 


LNU214
BamHI, XhoI
LNU214_F_BamHI(SEQ IN NO: 4915)




AAAGGATCCACTTCAAAGTACAAATTCCATTTCG




LNU214_F_BamHI(SEQ IN NO: 4915)




AAAGGATCCACTTCAAAGTACAAATTCCATTTCG




LNU214_NR_XhoI(SEQ IN NO: 4916)




AAACTCGAGCCACAGTAAACAAAGCATTCAAATC




LNU214_ER_XhoI(SEQ IN NO: 4917)




AAACTCGAGACCCAAACTCGAACAATTTACC


 


LNU215
BamHI, XhoI
LNU215_EF_BamHI(SEQ IN NO: 4918)




AAAGGATCCTTCCTGAGAAAGAAAGCGAAAC




LNU215_ER_XhoI(SEQ IN NO: 4919)




AAACTCGAGTCCAATCCCATAAGAACTGAGC


 


LNU216
BamHI, KpnI
LNU216_F_BamHI(SEQ IN NO: 4920)




AAAGGATCCACCCAAGCTCACACATCTCC




LNU216_F_BamHI(SEQ IN NO: 4920)




AAAGGATCCACCCAAGCTCACACATCTCC




LNU216_NR_KpnI(SEQ IN NO: 4921)




AAAGGTACCATACGAGGAAACCATGACGAAC




LNU216_ER_KpnI(SEQ IN NO: 4922)




AAAGGTACCCGATTACCCGATTTGTGATTTC


 


LNU217
BamHI, XhoI
LNU217_NF_BamHI(SEQ IN NO: 4923)




AAAGGATCCCGTTCGATCTCTCCCATATAATTC




LNU217_EF_BamHI(SEQ IN NO: 4924)




AAAGGATCCATCCATCACTCGTTCGATCTC




LNU217_NR_XhoI(SEQ IN NO: 4925)




AAACTCGAGCTAAATAAAGCATGGCAGACTGTGTC




LNU217_ER_XhoI(SEQ IN NO: 4926)




AAACTCGAGAGCTCCATCTATTCATTCGTGC


 


LNU218
SalI, XbaI
LNU218_NF_SalI(SEQ IN NO: 4927)




AAAGTCGACAGAAATCTGACCGCAATAATGG




LNU218_EF_SalI(SEQ IN NO: 4928)




AAAGTCGACTCTACAGAGAAATCTGACCGCA




LNU218_NR_XbaI(SEQ IN NO: 4929)




AAATCTAGAGATGTATAGCAACAAGAGAAACAAACA




LNU218_ER_XbaI(SEQ IN NO: 4930)




AAATCTAGAGAATGATGTATAGCAACAAGAGAAACA


 


LNU219
BamHI, XhoI
LNU219_EF_BamHI(SEQ IN NO: 4931)




AAAGGATCCGATGAAGAAGACATCAATGACTGG




LNU219_EF_BamHI(SEQ IN NO: 4931)




AAAGGATCCGATGAAGAAGACATCAATGACTGG


 


LNU220
SalI, XbaI
LNU220_NF_SalI(SEQ IN NO: 4932)




AAAGTCGACAACCTCGAACTCGAAGCAGAG




LNU220_EF_SalI(SEQ IN NO: 4933)




AAAGTCGACGAGAGAGACACAAGCCAACCTC




LNU220_NR_XbaI(SEQ IN NO: 4934)




AAATCTAGAAACAACCACCTCCATCACGTAG




LNU220_ER_XbaI(SEQ IN NO: 4935)




AAATCTAGACATCCAGCCACTAAATCGTTG


 


LNU223 
XhoI, EcoRV
LNU223_F_XhoI(SEQ IN NO: 4936)




AAACTCGAGATTAGAAGCCTTCGGCTTTACG




LNU223_F_XhoI(SEQ IN NO: 4936)




AAACTCGAGATTAGAAGCCTTCGGCTTTACG




LNU223_NR_EcoRV(SEQ IN NO: 4937)




AAAGATATCTCTACCTGAGAAATGCTCCCTG




LNU223_ER_EcoRV(SEQ IN NO: 4938)




AAAGATATCGTGAATATCATGGGTTGACTGG


 


LNU224 
BamHI, KpnI
LNU224_NF_BamHI(SEQ IN NO: 4939)




AAAGGATCCATCAACTCGCGACGAATCAG




LNU224_EF_BamHI(SEQ IN NO: 4940)




AAAGGATCCGAACAGAGAAGAGCATCAACTCG




LNU224_NR_KpnI(SEQ IN NO: 4941)




AAAGGTACCTATCCCTTGAGTTATTGCCTCG




LNU224_ER_KpnI(SEQ IN NO: 4942)




AAAGGTACCTCCTGCTACAATGCTATCCACC


 


LNU225 
BamHI, KpnI
LNU225_NF_BamHI(SEQ IN NO: 4943)




AAAGGATCCTCGGTAAGATTTCTTGGAGCAG




LNU225_EF_BamHI(SEQ IN NO: 4944)




AAAGGATCCTTCCTCTTCATCATCGACATCC




LNU225_NR_KpnI(SEQ IN NO: 4945)




AAAGGTACCCTAGCATAACCACGAGAGAGAAAGAG




LNU225_ER_KpnI(SEQ IN NO: 4946)




AAAGGTACCCTAACAAATGCATAACCACGAGAGAG


 


LNU228
SalI, XbaI
LNU228_NF_SalI(SEQ IN NO: 4947)




AAAGTCGACAACTCTTGCCACACGGGTC




LNU228_EF_SalI(SEQ IN NO: 4948)




AAAGTCGACAGAAACTCTTGCCACACAGCTC




LNU228_NR_XbaI(SEQ IN NO: 4949)




AAATCTAGACTATGTGTGTTTCTGCAGGACTTG




LNU228_ER_XbaI(SEQ IN NO: 4950)




AAATCTAGACTACGTACAAACTCTTATGGCGTGG


 


LNU229
SalI, SacI
LNU229_F_SalI(SEQ IN NO: 4951)




TAAGTCGACAATTTAGCAATGCTCTGTTTCTCTC




LNU229_ER_SacI(SEQ IN NO: 4952)




ACAGAGCTCAATCATTGATAACACTAAAACTTTCTGC


 


LNU23 
BamHI, KpnI
LNU23_EF_BamHI(SEQ IN NO: 4953)




AAAGGATCCAAAGTCAGCGTGAGAGACTGG




LNU23_ER_KpnI(SEQ IN NO: 4954)




AAAGGTACCCCTTGACACTTCAGATCTATTCTGTG


 


LNU230
BamHI, KpnI
LNU230_F_BamHI(SEQ IN NO: 4955)




TTCGGATCCACCACCCTCACACACGCT




LNU230_F_BamHI(SEQ IN NO: 4955)




TTCGGATCCACCACCCTCACACACGCT




LNU230_NR_KpnI(SEQ IN NO: 4956)




AAAGGTACCAAAGTACACCACAGTAGGCGAGA




LNU230_ER_KpnI(SEQ IN NO: 4957)




AAAGGTACCAAATATGACTGAATTAAGCAGCGAG


 


LNU232

LNU232_NF_SalI(SEQ IN NO: 4958)




AAAGTCGACACACACTTGGAAGCAAACAACC




LNU232_NR_XbaI(SEQ IN NO: 4959)




AAATCTAGAGAGACATACACTCCCACTGTCG


 


LNU233 
SalI, XbaI
LNU233_EF_SalI(SEQ IN NO: 4960)




AAAGTCGACGCGACCAGATCAGACTCCATC




LNU233_ER_XbaI(SEQ IN NO: 4961)




TTTTCTAGAGCTTCAAGCGATCCAGACC


 


LNU234
SalI, BamHI
LNU234_F_SalI(SEQ IN NO: 4962)




AAAGTCGACACGTTGAACGATGGGAGC




LNU234_ER_BamHI(SEQ IN NO: 4963)




AAAGGATCCAGGGATGTAACATTCAACCACC


 


LNU235
SalI, BamHI
LNU235_F_SalI(SEQ IN NO: 4964)




AAAGTCGACCACAATTCACCACCATGAACTC




LNU235_ER_BamHI(SEQ IN NO: 4965)




AAAGGATCCTGGAGCTAAACTTTATTGGTCACG


 


LNU236
BamHI, XhoI
LNU236_EF_BamHI(SEQ IN NO: 4966)




AAAGGATCCTTAACTGCTTGCCCGTTCAT




LNU236_ER_XhoI(SEQ IN NO: 4967)




AAACTCGAGGTCTAGAGAGGGAGACGATTGC


 


LNU239
BamHI, XhoI
LNU239_EF_BamHI(SEQ IN NO: 4968)




AAAGGATCCGTTCCTCCTCCTACCTTTGGTC




LNU239_ER_XhoI(SEQ IN NO: 4969)




AAACTCGAGATGTCCCGTCAACTGAAACAAC


 


LNU24
BamHI, XhoI
LNU24_NF_BamHI(SEQ IN NO: 4970)




AAAGGATCCCCTCTGGTCCTCCATCAGATAC




LNU24_EF_BamHI(SEQ IN NO: 4971)




AAAGGATCCCTACTTGCTGCTTGGCGTAGAC




LNU24_NR_XhoI(SEQ IN NO: 4972)




AAACTCGAGTGTGACGGTGCTAAAGACAATC




LNU24_ER_XhoI(SEQ IN NO: 4973)




AAACTCGAGTTAGTTGCGAGGACATCAAATC


 


LNU241 
SalI, XbaI
LNU241_NF2_SalI(SEQ IN NO: 4974)




TTAGTCGACCTTCGATATCATGGCGTCC




LNU241_EF2_SalI(SEQ IN NO: 4975)




AATGTCGACTGTCCATATACACTCCTTGCACTC




LNU241_NR2_XbaI(SEQ IN NO: 4976)




TAATCTAGACGATCTACACAAGAATCAATGGACA




LNU241_ER2_XbaI(SEQ IN NO: 4977)




TAATCTAGAGCTCCATGGATGTATGAGACC


 


LNU242
SalI, BamHI
LNU242_F_SalI(SEQ IN NO: 4978)




AAAGTCGACGAGAAAAATGTCCATTAGAGCTCAAG




LNU242_F_SalI(SEQ IN NO: 4978)




AAAGTCGACGAGAAAAATGTCCATTAGAGCTCAAG




LNU242_NR_BamHI(SEQ IN NO: 4979)




TTTGGATCCCTATATTAACACTACCTCAGCTTGGCT




LNU242_ER_BamHI(SEQ IN NO: 4980)




TGTGGATCCTGGTATATTCAAAGCCTAAAGATTCAG


 


LNU243
SalI, XbaI
LNU243_F_SalI(SEQ IN NO: 4981)




AAAGTCGACGACGGGTCAATCATGGAGG




LNU243_F_SalI(SEQ IN NO: 4981)




AAAGTCGACGACGGGTCAATCATGGAGG




LNU243_NR_XbaI(SEQ IN NO: 4982)




AAATCTAGACTATCAGATCACCCTCAACCTTACC




LNU243_ER_XbaI(SEQ IN NO: 4983)




AAATCTAGAGGAGTACACACAACACAACACG


 


LNU244
BamHI, EcoRV
LNU244_NF_BamHI(SEQ IN NO: 4984)




TTAGGATCCGGGAAGCGAGCATTATGGAC




LNU244_EF_BamHI(SEQ IN NO: 4985)




TTTGGATCCCCCTCCTTTCATTATTCTATCCG




LNU244_NR_EcoRV(SEQ IN NO: 4986)




AAAGATATCTGTTTCATCTGCACTACTTTACCTAGC




LNU244_ER_EcoRV(SEQ IN NO: 4987)


 


LNU245
XhoI, SacI
AAAGATATCGGCAAATAACCAAATGTCTCG




LNU245_EF_XhoI(SEQ IN NO: 4988)




AAACTCGAGCATCATCTTCTTCTTCTTCTCATTGG




LNU245_ER_SacI(SEQ IN NO: 4989)




AATGAGCTCCTAGAGATACAACAGACATGTGATCATTG


 


LNU246
BamHI, XhoI
LNU246_F_BamHI(SEQ IN NO: 4990)




AAAGGATCCATAATTTGGAATTGGGTTGCTG




LNU246_ER_XhoI(SEQ IN NO: 4991)




AAACTCGAGATGGTCTAACCAATATGGGACG


 


LNU247

LNU247_EF_BamHI(SEQ IN NO: 4992)




AAAGGATCCTCTTGCCCATTATTCTCCATTG




LNU247_ER_KpnI(SEQ IN NO: 4993)




AAAGGTACCTATCCGTCTGGTTGTTCATCG


 


LNU249
XhoI, SacI
LNU249_EF_XhoI(SEQ IN NO: 4994)




AAACTCGAGGCTCTTGAATTCTCCCTCATACC




LNU249_ER_SacI(SEQ IN NO: 4995)




AAAGAGCTCGGCAACTTAGCATTTGTGATGC


 


LNU25 
BamHI, XhoI
LNU25_EF_BamHI(SEQ IN NO: 4996)




AAAGGATCCATTGACTAGAGCGGGAGGAAAG




LNU25_ER_XhoI(SEQ IN NO: 4997)




AAACTCGAGCAACAATTGCTAAATTCATGGTG


 


LNU250
SalI, BamHI
LNU250_NF_SalI(SEQ IN NO: 4998)




AAAGTCGACTATCTCCACCGTTTGTTTGTTG




LNU250_EF_SalI(SEQ IN NO: 4999)




AAAGTCGACTTCATCATCATCGTATCTCCACC




LNU250_NR_BamHI(SEQ IN NO: 5000)




AAAGGATCCGGGTTTAATGGGTTAGTGAATTCTTC




LNU250_ER_BamHI(SEQ IN NO: 5001)




AAAGGATCCAACGCCAAATAACGCAAACTC


 


LNU251
BamHI, XhoI
LNU251_NF_BamHI(SEQ IN NO: 5002)




AAAGGATCCCCGAGGAAACCCTAATTTCTTG




LNU251_EF_BamHI(SEQ IN NO: 5003)




AAAGGATCCGTTTGCTGTATCTCCTCCATCG




LNU251_NR_XhoI(SEQ IN NO: 5004)




AAACTCGAGGATAACGTTGACATGTTCCATTTG




LNU251_ER_XhoI(SEQ IN NO: 5005)




AAACTCGAGAAACCCTATCATCCAATTCAGG


 


LNU253
BamHI, XhoI
LNU253_EF_BamHI(SEQ IN NO: 5006)




AAAGGATCCTATTCCGTGCAAACACAACAAC




LNU253_ER_XhoI(SEQ IN NO: 5007)




AAACTCGAGAACTGCACTTTCCCTCCTCTTC


 


LNU254
BamHI, XhoI
LNU254_NF_BamHI(SEQ IN NO: 5008)




AAAGGATCCATTTCTTCTCGCACTCTTCACC




LNU254_EF_BamHI(SEQ IN NO: 5009)




AAAGGATCCCCCTCTAAATAGGGAGTGAGTGAG




LNU254_R_XhoI(SEQ IN NO: 5010)




AAACTCGAGAACTTCAAACTTCCCAACCAAAC




LNU254_R_XhoI(SEQ IN NO: 5010)




AAACTCGAGAACTTCAAACTTCCCAACCAAAC


 


LNU255
SalI, BamHI
LNU255_F_SalI(SEQ IN NO: 5011)




AAAGTCGACAACAAACTTTAAACAATGGCTGG




LNU255_F_SalI(SEQ IN NO: 5011)




AAAGTCGACAACAAACTTTAAACAATGGCTGG




LNU255_NR_BamHI(SEQ IN NO: 5012)




AAAGGATCCGTTCTGGGTCAATGGTCGTATC




LNU255_ER_BamHI(SEQ IN NO: 5013)




AAAGGATCCTTATTGGATATGATTGGCCCAC


 


LNU256
BamHI, XhoI
LNU256_F_BamHI(SEQ IN NO: 5014)




AAAGGATCCGCTTGAAGCTTCCCACAACTAC




LNU256_F_BamHI(SEQ IN NO: 5014)




AAAGGATCCGCTTGAAGCTTCCCACAACTAC




LNU256_NR_XhoI(SEQ IN NO: 5015)




AAACTCGAGACGGCCTGATAAGCATTCAC




LNU256_ER_XhoI(SEQ IN NO: 5016)




AAACTCGAGGGAACATGTGACATAACAACAAGG


 


LNU257
SalI, XbaI
LNU257_EF_SalI(SEQ IN NO: 5017)




TTAGTCGACACTACTATGTACAGATTCAGCAACACAG




LNU257_ER_XbaI(SEQ IN NO: 5018)




AAATCTAGACATATACTCATGCACTCGTAGCA


 


LNU258
BamHI, KpnI
LNU258_F_BamHI(SEQ IN NO: 5019)




AAAGGATCCCCCACAAACAAACAAAATGGA




LNU258_ER_KpnI(SEQ IN NO: 5020)




AAAGGTACCAGCCCACTAACCCGGTG


 


LNU260
BamHI, XhoI
LNU260_NF_BamHI(SEQ IN NO: 5021)




AAAGGATCCGAACAATCCCAAGATTCTCCTC




LNU260_EF_BamHI(SEQ IN NO: 5022)




AAAGGATCCGTTACACGCGTAGGCTAGTGG




LNU260_NR_XhoI(SEQ IN NO: 5023)




AAACTCGAGCACTCAAAGAGAAGAGTGACAGAGTG




LNU260_ER_XhoI(SEQ IN NO: 5024)




AAACTCGAGCATTCACTCAAAGAGAAGAGTGACAG


 


LNU261
BamHI, XhoI
LNU261_F_BamHI(SEQ IN NO: 5025)




AATGGATCCTCCGAAACGAGAAAACAAACTATG




LNU261_ER_XhoI(SEQ IN NO: 5026)




TTTCTCGAGCCGATCAAGATTATTTCAGGACG


 


LNU263 
SalI, XbaI
LNU263_NF_SalI(SEQ IN NO: 5027)




AAAGTCGACTCTCAATCTCTCAATCCCGAGT




LNU263_EF_SalI(SEQ IN NO: 5028)




AAAGTCGACGGACTCAAGCTCACAATCACAA




LNU263_R_XbaI(SEQ IN NO: 5029)




AATTCTAGATTTCCAAGCTTATCTAGTGCATAACA




LNU263_R_XbaI(SEQ IN NO: 5029)




AATTCTAGATTTCCAAGCTTATCTAGTGCATAACA


 


LNU266
BamHI, XhoI
LNU266_NF_BamHI(SEQ IN NO: 5030)




AAAGGATCCGAGGAGCTTATCCTGTGCAGC




LNU266_EF_BamHI(SEQ IN NO: 5031)




AAAGGATCCAACGGAGACCACGTGTGAG




LNU266_NR_XhoI(SEQ IN NO: 5032)




AAACTCGAGCGCTGCATTGTTTCAAGAATTAC




LNU266_ER_XhoI(SEQ IN NO: 5033)




AAACTCGAGTAATGAGTTTATAGCCCGCTGC


 


LNU267 
SalI, XbaI
LNU267_NF_SalI(SEQ IN NO: 5034)




AAAGTCGACGATTCCAGTCCTCCTCCTGTTC




LNU267_EF_SalI(SEQ IN NO: 5035)




AAAGTCGACGTCTGGTGTGTGGATGATTCC




LNU267_NR_XbaI(SEQ IN NO: 5036)




AAATCTAGAATCTTCCATCATCATGCGCTAC




LNU267_ER_XbaI(SEQ IN NO: 5037)




AAATCTAGATCCGAAATGCTTGTATCATGG


 


LNU268
XhoI, EcoRV
LNU268_F2_XhoI(SEQ IN NO: 5038)




TATCTCGAGCATCCAATTCCACTTCCACAC




LNU268_R2_EcoRV(SEQ IN NO: 5039)




ATTGATATCCCACATTCAGATCCTCAGTGC


 


LNU27
SalI, XbaI
LNU27_F_SalI(SEQ IN NO: 5040)




AAAGTCGACAGCAGAAGGGATCGGAGATG




LNU27_ER_XbaI(SEQ IN NO: 5041)




AAATCTAGACATGAAATGAACCTCAGCAGTC


 


LNU271
BamHI, KpnI
LNU271_EF_BamHI(SEQ IN NO: 5042)




AAAGGATCCGCAGAATCGCAGTGCAGAC




LNU271_ER_KpnI(SEQ IN NO: 5043)




AAAGGTACCCACCAGAAATCACCAATGTGTC


 


LNU274 
SalI, XbaI
LNU274_F_SalI(SEQ IN NO: 5044)




AAAGTCGACTGATCGCCGTTGGATCTC




LNU274_F_SalI(SEQ IN NO: 5044)




AAAGTCGACTGATCGCCGTTGGATCTC




LNU274_ER_XbaI(SEQ IN NO: 5045)




AAATCTAGAATCTCTGGGTTGAGCGTTACTG




LNU274_ER_XbaI(SEQ IN NO: 5045)




AAATCTAGAATCTCTGGGTTGAGCGTTACTG


 


LNU276
BamHI, KpnI
LNU276_EF_BamHI(SEQ IN NO: 5046)




AAAGGATCCACACAAGCATCTCCTCTTCTCC




LNU276_ER_KpnI(SEQ IN NO: 5047)




AAAGGTACCAGAAGTGTGTATTTGTGCCGTG


 


LNU277
XhoI, EcoRV
LNU277_NF_XhoI(SEQ IN NO: 5048)




AAACTCGAGAAGGAATTTGTTTGTTGAAGCTG




LNU277_EF_XhoI(SEQ IN NO: 5049)




AAACTCGAGTTCGGTTCATCAAATCAGAAGG




LNU277_NR_EcoRV(SEQ IN NO: 5050)




AAAGATATCATTGCTGGTATCCTGAATCCTG




LNU277_ER_EcoRV(SEQ IN NO: 5051)




AAAGATATCAATGAAATCGTGTGCCAAATTC


 


LNU278

LNU278_NF_SalI(SEQ IN NO: 5052)




AAAGTCGACCCAAGAACACAACATCAAGAGC




LNU278_EF_SalI(SEQ IN NO: 5053)




AAAGTCGACCACCAGACCACTCAGAGAAGTG




LNU278_NR_XbaI(SEQ IN NO: 5054)




AAATCTAGATGAAACAAATAGAGTACCGCAGG




LNU278_ER_XbaI(SEQ IN NO: 5055)




AAATCTAGAATTATGCAGACCTGGAAGAAGC


 


LNU279 
SalI, XbaI
LNU279_NF_SalI(SEQ IN NO: 5056)




AAAGTCGACGCTTCAACACAACACTTCAGTAAATC




LNU279_EF_SalI(SEQ IN NO: 5057)




AAAGTCGACTATATGCGTCTGCTTCAACACAAC




LNU279_R_XbaI(SEQ IN NO: 5058)




AAATCTAGACATCAATCATTGGCTCATTGC




LNU279_R_XbaI(SEQ IN NO: 5058)




AAATCTAGACATCAATCATTGGCTCATTGC


 


LNU28
SalI, XbaI
LNU28_EF_SalI(SEQ IN NO: 5059)




AAAGTCGACAGCTGAACCACAAGCAGTGAG




LNU28_ER_XbaI(SEQ IN NO: 5060)




AAATCTAGACACAGTAGGCACCGAAAGTATG


 


LNU280
BamHI, XhoI
LNU280_F_BamHI(SEQ IN NO: 5061)




AAAGGATCCCCATCGCTTCGTTTCCAAG




LNU280_F_BamHI(SEQ IN NO: 5061)




AAAGGATCCCCATCGCTTCGTTTCCAAG




LNU280_NR_XhoI(SEQ IN NO: 5062)




AAACTCGAGATCGTTTCTTCCACTCCACTTC




LNU280_ER_XhoI(SEQ IN NO: 5063)




AAACTCGAGGTTACTTCTGGACTGCACAACG


 


LNU284
BamHI, XhoI
LNU284_NF_BamHI(SEQ IN NO: 5064)




AAAGGATCCGCATAGTCTCTGGTGCAAGAATC




LNU284_EF_BamHI(SEQ IN NO: 5065)




AAAGGATCCGACAGCATAGTCTCTGGTGCAAG




LNU284_NR_XhoI(SEQ IN NO: 5066)




AAACTCGAGCAATTCTACAATGGGCCTTGAC




LNU284_ER_XhoI(SEQ IN NO: 5067)




AAACTCGAGTGATATATGTTAATGCTCACCCAATTC


 


LNU287
BamHI, XhoI
LNU287_EF_BamHI(SEQ IN NO: 5068)




AAAGGATCCATTTATCGGGTCTCTTCCCAAC




LNU287_ER_XhoI(SEQ IN NO: 5069)




AAACTCGAGTGGATGCCTGTAGCTTGAATTAC


 


LNU288
BamHI, XhoI
LNU288_EF_BamHI(SEQ IN NO: 5070)




AAAGGATCCGCAAAGTTGTAGCCAATGGAAG




LNU288_ER_XhoI(SEQ IN NO: 5071)




AAACTCGAGGCAAACTTGAGTTGCATTTGAC


 


LNU289
BamHI, XhoI
LNU289_EF_BamHI(SEQ IN NO: 5072)




AAAGGATCCCCCTTCTCTCCATAACGAACC




LNU289_ER_XhoI(SEQ IN NO: 5073)




AAACTCGAGCTTCTTCTCGGGTTAAAGGGAC


 


LNU29 
BamHI, KpnI
LNU29_F_BamHI(SEQ IN NO: 5074)




TTTGGATCCTCATTATCATCAATATGGGTAGAGCTC




LNU29_F_BamHI(SEQ IN NO: 5074)




TTTGGATCCTCATTATCATCAATATGGGTAGAGCTC




LNU29_NR_KpnI(SEQ IN NO: 5075)




TTTGGTACCTTAACCTCTTTCGAACTGTTTGTTTG




LNU29_ER_KpnI(SEQ IN NO: 5076)




ACTGGTACCTTACAACATGTCCAAAAGCTCAGAG


 


LNU3 
SalI, XbaI
LNU3_NF_SalI(SEQ IN NO: 5077)




AAAGTCGACAACCACCTGCGATTCTGC




LNU3_EF_SalI(SEQ IN NO: 5078)




AAAGTCGACGTTCGTCCGCAACAAACC




LNU3_NR_XbaI(SEQ IN NO: 5079)




AAATCTAGACTAGAGCGGCAATATCATAGCAAAC




LNU3_ER_XbaI(SEQ IN NO: 5080)




AAATCTAGACTACATCCATGTACAAGCTAACATGC


 


LNU33 
BamHI, XhoI
LNU33_EF_BamHI(SEQ IN NO: 5081)




AAAGGATCCTGGCGGCTAAAGTTTGTGAG




LNU33_ER_XhoI(SEQ IN NO: 5082)




AAACTCGAGTTACACATGGAAGACCTGAACG


 


LNU35

LNU35_NF_BamHI(SEQ IN NO: 5083)




AAAGGATCCCGGTACAATAGCCGAATTTGAG




LNU35_EF_BamHI(SEQ IN NO: 5084)




AAAGGATCCAAGCCCAGCCGGTACAATAG




LNU35_NR_XhoI(SEQ IN NO: 5085)




AAACTCGAGGACTCCAATCAATCTACGGAGC




LNU35_ER_XhoI(SEQ IN NO: 5086)




AAACTCGAGTGGCACTTTCTAGCAGCCTAAC


 


LNU36
BamHI, XhoI
LNU36_EF_BamHI(SEQ IN NO: 5087)




AAAGGATCCCCCAAACTCCCTTGTGAACTG




LNU36_ER_XhoI(SEQ IN NO: 5088)




AAACTCGAGTCTAATGTACAACACTGGGCAAAC


 


LNU37
BamHI, XhoI
LNU37_EF_BamHI(SEQ IN NO: 5089)




AAAGGATCCACCAAGTCGCTCAAAGAAACTG




LNU37_ER_XhoI(SEQ IN NO: 5090)




AAACTCGAGGCACGGTACCTTCTTGTTCTTC


 


LNU4 
BamHI, XhoI
LNU4_NF_BamHI(SEQ IN NO: 5091)




AAAGGATCCAGATAGACCCGGAGGAAACAAG




LNU4_EF_BamHI(SEQ IN NO: 5092)




AAAGGATCCAGGCGCCAACTAATAACCAGAG


 


LNU4 
BamHI, XhoI
LNU4_NR_XhoI(SEQ IN NO: 5093)




AAACTCGAGCCCTAGAATAAGAACCACAAATCG




LNU4_ER_XhoI(SEQ IN NO: 5094)




AAACTCGAGTTCAGAAATTCAAATCACAGTTTCC


 


LNU40
SalI, XbaI
LNU40_EF_SalI(SEQ IN NO: 5095)




AAAGTCGACACCACATATCATCGAGAGTTCG




LNU40_ER_XbaI(SEQ IN NO: 5096)




AAATCTAGACTTGGTCACTGGCTAGACCATC


 


LNU43
BamHI, XhoI
LNU43_EF_BamHI(SEQ IN NO: 5097)




AAAGGATCCTCTCTCCGACCTAGCGAGAC




LNU43_ER_XhoI(SEQ IN NO: 5098)




AAACTCGAGTAATTTCAAGGTCTTGCCCAAC


 


LNU44
SalI, XbaI
LNU44_EF_SalI(SEQ IN NO: 5099)




AAAGTCGACCTACCAACTCCGGCTTGTTC




LNU44_ER_XbaI(SEQ IN NO: 5100)




AAATCTAGACCTTCACACTGATCACTCGTTTC


 


LNU45
SalI, XbaI
LNU45_F_SalI(SEQ IN NO: 5101)




AAAGTCGACCGCTTCTTTCTCACAATGGTTC




LNU45_ER_XbaI(SEQ IN NO: 5102)




AAATCTAGATGGCTACTATGCCTTGTGGAAG


 


LNU46
BamHI, SalI
LNU46_EF_SalI(SEQ IN NO: 5103)




AAAGTCGACAGGTTGAAGAAGCAACACAAGC




LNU46_ER_BamHI(SEQ IN NO: 5104)




AAAGGATCCAACACTTCGAATCATGACCTCC


 


LNU48
BamHI, XhoI
LNU48_EF_BamHI(SEQ IN NO: 5105)




AAAGGATCCGATATGGTACAACAACCGAGAGC




LNU48_ER_XhoI(SEQ IN NO: 5106)




AAACTCGAGGATCATCCCATTTCAAGAGAGC


 


LNU5 
BamHI, XhoI
LNU5_NF_BamHI(SEQ IN NO: 5107)


(6669)

AAAGGATCCGCTCTCCACTGTCCACGAAC




LNU5_EF_BamHI(SEQ IN NO: 5108)




AAAGGATCCCCAGATTAGTTGGAAGCTTTCTCTTC




LNU5_NR_XhoI(SEQ IN NO: 5109)




AAACTCGAGTCATCATCTTCCATTGCTCTACC




LNU5_ER_XhoI(SEQ IN NO: 5110)




AAACTCGAGTTCCTTTCATTATTTGCCAACG


 


LNU5
BamHI, XhoI
LNU5_NF_BamHI(SEQ IN NO: 5107)


(Root_P_F)

AAAGGATCCGCTCTCCACTGTCCACGAAC




LNU5_EF_BamHI(SEQ IN NO: 5108)




AAAGGATCCCCAGATTAGTTGGAAGCTTTCTCTTC




LNU5_NR_XhoI(SEQ IN NO: 5109)




AAACTCGAGTCATCATCTTCCATTGCTCTACC




LNU5_ER_XhoI(SEQ IN NO: 5110)




AAACTCGAGTTCCTTTCATTATTTGCCAACG


 


LNU50
SalI, XbaI
LNU50_NF_SalI(SEQ IN NO: 5111)




AAAGTCGACAGAACTGATAAGGCCAGTGTCG




LNU50_EF_SalI(SEQ IN NO: 5112)




AAAGTCGACCGCGGTTAAGTTAGTTGTTGTTG




LNU50_NR_XbaI(SEQ IN NO: 5113)




AAATCTAGATCCTATGTGTTGTTTAGCTACCAGG




LNU50_ER_XbaI(SEQ IN NO: 5114)




AAATCTAGATTCGCATTTAAATAACTGTGCTG


 


LNU52
XhoI, EcoRV
LNU52_NF_XhoI(SEQ IN NO: 5115)




AAACTCGAGGGTGGGTGTTGCTTGTACC




LNU52_NR_EcoRV(SEQ IN NO: 5116)




AAAGATATCCCTCACCAGGTAGGTAGTCTGC


 


LNU53
BamHI, XhoI
LNU53_NF_BamHI(SEQ IN NO: 5117)




AAAGGATCCGAGTGAATTAGAGAAAGGCAAATGG




LNU53_EF_BamHI(SEQ IN NO: 5118)




AAAGGATCCATCGAGTGAATTAGAGAAAGGCA




LNU53_R_XhoI(SEQ IN NO: 5119)




AGTCTCGAGTTATGGAAGTTATGAAATACAAGCAGG




LNU53_R_XhoI(SEQ IN NO: 5119)




AGTCTCGAGTTATGGAAGTTATGAAATACAAGCAGG


 


LNU54
SalI, XbaI
LNU54_EF_SalI(SEQ IN NO: 5120)




AAAGTCGACCAGTTGGCCTTATCCGTATCTC




LNU54_R_XbaI(SEQ IN NO: 5121)




AAATCTAGAATCCTTGAATCCAAATGAAACG


 


LNU55
BamHI, XhoI
LNU55_F_BamHI(SEQ IN NO: 5122)




AAAGGATCCTGTTAGGGAAGAAGTACTGAAGGTG




LNU55_F_BamHI(SEQ IN NO: 5122)




AAAGGATCCTGTTAGGGAAGAAGTACTGAAGGTG




LNU55_NR_XhoI(SEQ IN NO: 5123)




AAACTCGAGGAGGACCAGTTGAATGCCTC




LNU55_ER_XhoI(SEQ IN NO: 5124)




AAACTCGAGACAGAACTCTAGACTCATGAGGACC


 


LNU56
SalI, XbaI
LNU56_EF_SalI(SEQ IN NO: 5125)




AAAGTCGACACATAACACCTCAAATTCTGTGATTC




LNU56_ER_XbaI(SEQ IN NO: 5126)




AAATCTAGATGGAGTACACAACAGATGGCTG


 


LNU57
SalI, XbaI
LNU57_NF_SalI(SEQ IN NO: 5127)




AAAGTCGACCTGAGGGACTGTTCCAGCTC




LNU57_EF_SalI(SEQ IN NO: 5128)




AAAGTCGACACACAAGAAACACCTGAGGGAC




LNU57_NR_XbaI(SEQ IN NO: 5129)




AAATCTAGACTATTTATTATCACAGCCATCCATCG




LNU57_ER_XbaI(SEQ IN NO: 5130)




AAATCTAGACTATTGGCAGGCACACTGATATG


 


LNU58

LNU58_NF_SalI(SEQ IN NO: 5131)




AAAGTCGACCCAAGCTAACGAGCAGTAGCAC




LNU58_EF_SalI(SEQ IN NO: 5132)




AAAGTCGACGATAGCCAAGCTAACGAGCAGTAG




LNU58_NR_XbaI(SEQ IN NO: 5133)




AAATCTAGAGTTCTTCTTCGTGGTTTCCAAC




LNU58_ER_XbaI(SEQ IN NO: 5134)




AAATCTAGAAGACCAGAAACATCTCCGTTTG


 


LNU6
BamHI, XhoI
LNU6_NF_BamHI(SEQ IN NO: 5135)




AAAGGATCCCAACACCGCTCATCTTCTCTTC




LNU6_NR_XhoI(SEQ IN NO: 5136)




AAACTCGAGCAACCGAAGGTGCTTATCGTC


 


LNU60

LNU60_NF_BamHI(SEQ IN NO: 5137)




AAAGGATCCACTCTGAACTGAAGCGAAGTCC




LNU60_NF_BamHI(SEQ IN NO: 5137)




AAAGGATCCACTCTGAACTGAAGCGAAGTCC




LNU60_NR_XhoI(SEQ IN NO: 5138)




AAACTCGAGTCTTCTCTGTTGCTGGAGAAGC




LNU60_NR_XhoI(SEQ IN NO: 5138)


 


LNU61

AAACTCGAGTCTTCTCTGTTGCTGGAGAAGC




LNU61_NF_BamHI(SEQ IN NO: 5139)




AAAGGATCCCCATTATATTGCTCGCGAGTTC




LNU61_NR_XhoI(SEQ IN NO: 5140)




AAACTCGAGCAGTAGCATCAAAGAAACCATCG


 


LNU63
SalI, XbaI
LNU63_EF_SalI(SEQ IN NO: 5141)




AAAGTCGACGATCTTATCCTCGGCGAAGC




LNU63_ER_XbaI(SEQ IN NO: 5142)




AAATCTAGAGCAGTAGGGATGTGTCAACAAG


 


LNU64
BamHI, XhoI
LNU64_NF_BamHI(SEQ IN NO: 5143)




AAAGGATCCTACCCTGTCTCTCCTCCAGC




LNU64_NR_XhoI(SEQ IN NO: 5144)




AAACTCGAGATTTCTGCGTACACCAAGAACC


 


LNU65
BamHI, XhoI
LNU65_F_BamHI(SEQ IN NO: 5145)




AAAGGATCCATTTCCTCCTTCCCTTGCAC




LNU65_ER_XhoI(SEQ IN NO: 5146)




AAACTCGAGAGGCATTTGAAACTGGTTGATG


 


LNU67

LNU67_F_XhoI(SEQ IN NO: 5147)




AAACTCGAGTTACTCTCTCCGCCCTCCTAAAC




LNU67_F_XhoI(SEQ IN NO: 5147)




AAACTCGAGTTACTCTCTCCGCCCTCCTAAAC




LNU67_NR_SacI(SEQ IN NO: 5148)




AAAGAGCTCCTGTTATTATGTGCTGCCAACG




LNU67_ER_SacI(SEQ IN NO: 5149)




AAAGAGCTCAGGAGTTCACCTTGGAGATCAG


 


LNU68
BamHI, KpnI
LNU68_EF_BamHI(SEQ IN NO: 5150)




AAAGGATCCTATACAGCCTAGAACAACTGCTTGC




LNU68_ER_KpnI(SEQ IN NO: 5151)




AAAGGTACCTCATGTCAGTATCTCAGGCGTC


 


LNU69
BamHI, XhoI
LNU69_EF_BamHI(SEQ IN NO: 5152)




AAAGGATCCCAGCAGCAGCTAGGGTTTAGAG




LNU69_ER_XhoI(SEQ IN NO: 5153)




AAACTCGAGAACAATGAAGATGGTCTCAATGC


 


LNU7
SalI, XbaI
LNU7_F_SalI(SEQ IN NO: 5154)




AAAGTCGACGAACTGCACCTTCACCTTCC




LNU7_ER_XbaI(SEQ IN NO: 5155)




AAATCTAGAGGTCTTCAGCAGAAACCAGG


 


LNU70
SalI, XbaI
LNU70_NF_SalI_Jap(SEQ IN NO: 5156)




AAAGTCGACATTCCACTCCACCTCGTCC




LNU70_EF_SalI_Jap(SEQ IN NO: 5157)




AAAGTCGACAATCCACTACTATTCCACTCCACC




LNU70_NR_XbaI_Jap(SEQ IN NO: 5158)




AAATCTAGACGGCAGTAGAGAAGTGCTATTG




LNU70_ER_XbaI_Jap(SEQ IN NO: 5159)




AAATCTAGAGCAATTTGGCATGGTAATGAG


 


LNU71
BamHI, KpnI
LNU71_NF_BamHI(SEQ IN NO: 5160)




AAAGGATCCTTCTCTCGTCTCGGCTCAAG




LNU71_EF_BamHI(SEQ IN NO: 5161)




AAAGGATCCCAAGTCTCGTGCTCTCACTCTC




LNU71_NR_KpnI(SEQ IN NO: 5162)




AAAGGTACCGCATGTGAATTACGAACCACAG




LNU71_ER_KpnI(SEQ IN NO: 5163)




AAAGGTACCAACGAAATTGTTGCTGGGATAG


 


LNU72
BamHI, XhoI
LNU72_NF_BamHI(SEQ IN NO: 5164)




AAAGGATCCGTTGGTCACCACCCAAACTC




LNU72_NR_XhoI(SEQ IN NO: 5165)




AAACTCGAGGCACTGGAGTACTGGACAAGTG


 


LNU73
XhoI, SacI
LNU73_EF_XhoI(SEQ IN NO: 5166)




AAACTCGAGACATATACACACCGGGCCAC




LNU73_ER_SacI(SEQ IN NO: 5167)




AAAGAGCTCCAAATGTTGCGTCGATCAAG


 


LNU74
SalI, XbaI
LNU74_NF_SalI(SEQ IN NO: 5168)




AAAGTCGACGCATCTTCCTTAGCTCGCTC




LNU74_EF_SalI(SEQ IN NO: 5169)




AAAGTCGACATTTCTGCATCTTCCTTAGCTCG




LNU74_NR_XbaI(SEQ IN NO: 5170)




AAATCTAGACTGGCCAGGTAAGAGTGACTTG




LNU74_ER_XbaI(SEQ IN NO: 5171)




AAATCTAGATGATCCACTAAGTAGCAGAACAAGG


 


LNU76
BamHI, KpnI
LNU76_EF_BamHI(SEQ IN NO: 5172)




AAAGGATCCAAATCATCAGCACAAGATCGAG




LNU76_ER_KpnI(SEQ IN NO: 5173)




AAAGGTACCCTACAATAGGATTAATTTGCCGCTG


 


LNU79
XhoI, EcoRV
LNU79_EF_XhoI(SEQ IN NO: 5174)




AAACTCGAGACGAGAATCGCTGAACCTCA




LNU79_R_EcoRV(SEQ IN NO: 5175)




AAAGATATCAAGGAATCAAACTCCAGTTCTCAA


 


LNU8
SalI, XbaI
LNU8_F_SalI(SEQ IN NO: 5176)




AAAGTCGACGGAAAAGAAGAGCTCAAGAAGAA




LNU8_F_SalI(SEQ IN NO: 5176)




AAAGTCGACGGAAAAGAAGAGCTCAAGAAGAA




LNU8_NR_XbaI(SEQ IN NO: 5177)




TTTTCTAGATCATGAGAGACCCACTTGAGGAG




LNU8_ER_XbaI(SEQ IN NO: 5178)




TATTCTAGATCAATTGTATGAGAGACCCACTTG


 


LNU81
SalI, XbaI
LNU81_NF_SalI(SEQ IN NO: 5179)




AAAGTCGACGGGACTGTTGAAGCTCTGC




LNU81_EF_SalI(SEQ IN NO: 5180)




AAAGTCGACGAAACACCTGAGGGACTGTTG




LNU81_NR_XbaI(SEQ IN NO: 5181)




AAATCTAGAACAGCACTGGTGGTTGAAGAAC




LNU81_ER_XbaI(SEQ IN NO: 5182)




AAATCTAGAAATTACAGCCATCCATCCAATC


 


LNU82
BamHI, XhoI
LNU82_NF_BamHI(SEQ IN NO: 5183)




AAAGGATCCGAACACGCTCTCTTCCAAAGC




LNU82_EF_BamHI(SEQ IN NO: 5184)




AAAGGATCCGAAGAACAAGACGAACACGCTC




LNU82_NR_XhoI(SEQ IN NO: 5185)




AAACTCGAGAGGCTACCAATCCACATCAGAC




LNU82_ER_XhoI(SEQ IN NO: 5186)




AAACTCGAGTATTCCAATCCAATCAACCAGG


 


LNU83
BamHI, KpnI
LNU83_F_BamHI(SEQ IN NO: 5187)




AAAGGATCCGAAGGAGGCAATGGCTCG




LNU83_ER_KpnI(SEQ IN NO: 5188)




AAAGGTACCAGGAGCTCGACTCAAACATCTG


 


LNU84
BamHI, KpnI
LNU84_F_BamHI(SEQ IN NO: 5189)




TTAGGATCCGAGCCCCATTCCATTCTTC




LNU84_F_BamHI(SEQ IN NO: 5189)




TTAGGATCCGAGCCCCATTCCATTCTTC




LNU84_NR_KpnI(SEQ IN NO: 5190)




AAAGGTACCCCAGCCAGGATCAGATCAAG




LNU84_ER_KpnI(SEQ IN NO: 5191)




TTAGGTACCATTTCCTTGTCAGGTCCAGC


 


LNU85
SalI, XbaI
LNU85_EF_SalI(SEQ IN NO: 5192)




AAAGTCGACATTTGGGACATCGTCTCCTTC




LNU85_ER_XbaI(SEQ IN NO: 5193)




AAATCTAGAAAACAAACAAGGCAGAGTCCAC


 


LNU86
BamHI, XhoI
LNU86_NF_BamHI(SEQ IN NO: 5194)




AAAGGATCCGGGACAGGACTGTTGGAAGTC




LNU86_EF_BamHI(SEQ IN NO: 5195)




AAAGGATCCAGCACACGTTCGTCTTCCTC




LNU86_NR_XhoI(SEQ IN NO: 5196)




AAACTCGAGCAGTGTTGTGGTAATGAGGTCG




LNU86_ER_XhoI(SEQ IN NO: 5197)




AAACTCGAGACTCAAGTGGCTCTCTCAGGAC


 


LNU87
SalI, XbaI
LNU87_EF_SalI(SEQ IN NO: 5198)




AAAGTCGACGATTTGAAGCTCCCAGTTCTTG




LNU87_ER_XbaI(SEQ IN NO: 5199)




AAATCTAGACTACAATGAAATGAAATCCTGGAAGG


 


LNU89
BamHI, XhoI
LNU89_NF_BamHI(SEQ IN NO: 5200)




AAAGGATCCCAACTCGAAAGGCTCCATTG




LNU89_EF_BamHI(SEQ IN NO: 5201)




AAAGGATCCGGTCTCCTCATCGAGTCCAAC




LNU89_NR_XhoI(SEQ IN NO: 5202)




AAACTCGAGCCGCAGCCAGGATTATATCAC




LNU89_ER_XhoI(SEQ IN NO: 5203)




AAACTCGAGCTTCAGCTTTGGAGAGCAACC


 


LNU9
SalI, XbaI
LNU9_NF_SalI(SEQ IN NO: 5204)




AAAGTCGACCGTGAGGTTAACAACAAGAACAAG




LNU9_EF_SalI(SEQ IN NO: 5205)




AAAGTCGACCAATTGCCAAGTACTCTCTGAGC




LNU9_NR_XbaI(SEQ IN NO: 5206)




AAATCTAGATCAGCACTGTCCATTCAGGTAG




LNU9_ER_XbaI(SEQ IN NO: 5207)




AAATCTAGACTCACGCTTGGATTGGATTAC


 


LNU94
SalI, SacI
LNU94_NF_SalI(SEQ IN NO: 5208)




TTTGTCGACTTAGGCATTATGGCTGTGGG




LNU94_EF_SalI(SEQ IN NO: 5209)




TTTGTCGACTTTGAACCAATTTTAGGCATTATGG




LNU94_NR_SacI(SEQ IN NO: 5210)




TTCGAGCTCTCATTTAATGATCTCATGCTTCTCC




LNU94_ER_SacI(SEQ IN NO: 5211)




TTCGAGCTCTGCAACAAACAAGCTTACACAATAC


 


LNU95
BamHI, XhoI
LNU95_NF_BamHI(SEQ IN NO: 5212)




AAAGGATCCAGAAACCTCGACCCATTTCAG




LNU95_EF_BamHI(SEQ IN NO: 5213)




AAAGGATCCCCTCCAAATTGTAATCTAAACCTGC




LNU95_NR_XhoI(SEQ IN NO: 5214)




AAACTCGAGCAAACACCACTCATGACTCCAC




LNU95_ER_XhoI(SEQ IN NO: 5215)




AAACTCGAGTTCTCCCAGCAAACTTTCTCAC


 


LNU96
SalI, XbaI
LNU96_EF_SalI(SEQ IN NO: 5216)




AAAGTCGACCGGTTCGTCTAGTCGTCTTCC




LNU96_ER_XbaI(SEQ IN NO: 5217)




AAATCTAGAAGCTCAGAGAAAGCAGGTTCAG


 


LNU98
BamHI, KpnI
LNU98_NF_BamHI(SEQ IN NO: 5218)




AAAGGATCCGAAGCGACTCCAAGAAGCAG




LNU98_EF_BamHI(SEQ IN NO: 5219)




AAAGGATCCCTCGTCTGTGTCTGCCTACTCC




LNU98_NR_KpnI(SEQ IN NO: 5220)




AAAGGTACCTGACACCATTCAGACCAAAGTG




LNU98_ER_KpnI(SEQ IN NO: 5221)




AAAGGTACCCCATGTCCTGGAAATATGTCTG





Table 58. Provided are the PCR primers used for cloning the genes of some embodiments of the invention. Fwd = forward primer; Rev = reverse primer; Nested = nested primer for PCR (internal primer); External = external primer for PCR. NF = nested forward; EF-external forward; NR-nested reverse; ER-external reverse.













TABLE 59







Genes cloned from cDNA libraries or genomic DNA in a High copy number plasmid















Polypeptide


Gene
High copy
Amplified from
Polynucleotide
SEQ ID












Name
plasmid
Organism
Origin
SEQ ID NO:
NO:





LNU1
pKS (Pks_J)
ARABIDOPSIS
cDNA
258
707




Arabidopsis




thaliana Kondara


LNU10
Topo B
RICE Oryza
cDNA
267
477




sativa L. Indica




Lebbonet


LNU100
pKS (Pks_J)
COTTON
cDNA
331
735




Gossypium




hirsutum ND


LNU101
pKS (Pks_J)
RICE Oryza
cDNA
332
736




sativa L. Japonica




LEMONT


LNU104
pGXN
SOYBEAN
cDNA
333
737



(pKG + Nos + 35S)
Glycine max 58-




261


LNU105
pKS (Pks_J)
WHEAT Triticum
cDNA
334
738




aestivum L.


LNU106
pGXN
WHEAT Triticum
cDNA
335
739



(pKG + Nos + 35S)
aestivum L. ND


LNU107
pKS (Pks_J)
RICE Oryza
cDNA
336
547




sativa L. Japonica




Nipponbare


LNU109
pKS (Pks_J)
RICE Oryza
cDNA
337
548




sativa L. Indica




Lebbonet


LNU11


GeneArt
268
478


LNU110
pKS (Pks_J)
RICE Oryza
cDNA
338
549




sativa L. Indica




TEBBONET


LNU112


GeneArt
339
550


LNU113
pKS (Pks_J)
MAIZE Zea mays
cDNA
340
740




L. OH43


LNU114
pGXN
RICE Oryza
cDNA
341
552



(pKG + Nos + 35S)
sativa L. Japonica




ND


LNU115
pKS (Pks_J)
RICE Oryza
cDNA
342
553




sativa L. Japonica




Nipponbare


LNU116
pKS (Pks_J)
RICE Oryza
cDNA
343
554




sativa L. Japonica




LEMONT


LNU117
pKS (Pks_J)
RICE Oryza
cDNA
344
555




sativa L. Japonica




ND


LNU118
pKS (Pks_J)
RICE Oryza
cDNA
345
556




sativa L. Indica




TEBBONET


LNU119
pKS (Pks_J)
RICE Oryza
cDNA
346
557




sativa L. Japonica




Nipponbare


LNU12
pKS (Pks_J)
RICE Oryza
cDNA
269
709




sativa L. Indica




Lebbonet


LNU120
pGXN
RICE Oryza
cDNA
347
558



(pKG + Nos + 35S)
sativa L. Japonica




LEMONT


LNU121
pGXNa
RICE Oryza
cDNA
348
559




sativa L. Japonica




Nipponbare


LNU122
Topo B
RICE Oryza
cDNA
349
560




sativa L. Indica




Lebbonet


LNU123
pKS (Pks_J)
ARABIDOPSIS
cDNA
350
741




Arabidopsis




thaliana Kondara


LNU124
pKS (Pks_J)
ARABIDOPSIS
cDNA
351
562




Arabidopsis




thaliana Kondara


LNU125
pGXN
ARABIDOPSIS
Genomic
466
—



(pKG + Nos + 35S)
Arabidopsis




thaliana Kondara


LNU126
pKS (Pks_J)
ARABIDOPSIS
cDNA
352
563




Arabidopsis




thaliana Kondara


LNU127


GeneArt
353
564


LNU128
pKS (Pks_J)
ARABIDOPSIS
cDNA
354
742




Arabidopsis




thaliana Kondara


LNU129
pKS (Pks_J)
ARABIDOPSIS
cDNA
355
743




Arabidopsis




thaliana Kondara


LNU13


GeneArt
270
480


LNU130
pGXN
ARABIDOPSIS
cDNA
356
744



(pKG + Nos + 35S)
Arabidopsis




thaliana Kondara


LNU131
pKS (Pks_J)
ARABIDOPSIS
cDNA
357
568




Arabidopsis




thaliana Kondara


LNU132
pGXN
ARABIDOPSIS
cDNA
358
569



(pKG + Nos + 35S)
Arabidopsis




thaliana Kondara


LNU133
pGXN
ARABIDOPSIS
cDNA
359
745



(pKG + Nos + 35S)
Arabidopsis




thaliana Kondara


LNU134
pKS (Pks_J)
ARABIDOPSIS
cDNA
360
746




Arabidopsis




thaliana Kondara


LNU135
pKS (Pks_J)
ARABIDOPSIS
cDNA
361
747




Arabidopsis




thaliana Kondara


LNU136
pKS (Pks_J)
ARABIDOPSIS
cDNA
362
573




Arabidopsis




thaliana Columbia




wt


LNU138
pKS (Pks_J)
BARLEY
cDNA
363
574




Hordeum vulgare




L. Manit


LNU14
pKS (Pks_J)
ARABIDOPSIS
cDNA
271
710




Arabidopsis




thaliana Kondara


LNU140
pGXN
ARABIDOPSIS
cDNA
364
748



(pKG + Nos + 35S)
Arabidopsis




thaliana Kondara


LNU141
pKS (Pks_J)
WHEAT Triticum
cDNA
365
749




aestivum L.


LNU142


GeneArt
366
577


LNU143
pKS (Pks_J)
COTTON
cDNA
367
750




Gossypium




hirsutum ND


LNU147
pKS (Pks_J)
COTTON
cDNA
368
580




Gossypium




hirsutum Akala


LNU148
pKS (Pks_J)
SOYBEAN
cDNA
369
695




Glycine max 58-




261


LNU149
pKS (Pks_J)
RICE Oryza
cDNA
370
751




sativa L. Japonica




ND


LNU15
pGXN
ARABIDOPSIS
cDNA
272
711



(pKG + Nos + 35S)
Arabidopsis




thaliana Kondara


LNU150
pGXN
COTTON
cDNA
371
752



(pKG + Nos + 35S)
Gossypium




hirsutum ND


LNU153
pKS (Pks_J)
RICE Oryza
cDNA
372
753




sativa L. Japonica




LEMONT


LNU154
pKS (Pks_J)
COTTON
cDNA
373
754




Gossypium




hirsutum ND


LNU155
pGXN
COTTON
cDNA
374
755



(pKG + Nos + 35S)
Gossypium




hirsutum ND


LNU157
pGXN
SOYBEAN
cDNA
375
587



(pKG + Nos + 35S)
Glycine max 58-




261


LNU158
pKS (Pks_J)
COTTON
cDNA
376
588




Gossypium




hirsutum Akala


LNU161
pGXN
SOYBEAN
cDNA
377
589



(pKG + Nos + 35S)
Glycine max 58-




261


LNU168
pGXNa
SORGHUM
cDNA
378
590




Sorghum bicolor




ND


LNU17
pKS (Pks_J)
RICE Oryza
cDNA
273
483




sativa L. Japonica




ND


LNU170
pGXN
ARABIDOPSIS
cDNA
379
756



(pKG + Nos + 35S)
Arabidopsis




thaliana Kondara


LNU171
pGXN
BARLEY
cDNA
380
757



(pKG + Nos + 35S)
Hordeum vulgare




L. Manit


LNU172
pKS (Pks_J)
BARLEY
cDNA
381
758




Hordeum vulgare




L. Manit


LNU173
pGXN
BARLEY
cDNA
382
594



(pKG + Nos + 35S)
Hordeum vulgare




L. ND


LNU175
pKS (Pks_J)
ARABIDOPSIS
cDNA
383
595




Arabidopsis




thaliana Kondara


LNU176
pGXN
RICE Oryza
cDNA
384
759



(pKG + Nos + 35S)
sativa L. Japonica




ND


LNU177
pGXN
ARABIDOPSIS
cDNA
385
597



(pKG + Nos + 35S)
Arabidopsis




thaliana Kondara


LNU178
pKS (Pks_J)
ARABIDOPSIS
cDNA
386
598




Arabidopsis




thaliana Kondara


LNU179
pKS (Pks_J)
ARABIDOPSIS
cDNA
387
760




Arabidopsis




thaliana Kondara


LNU180
pKS (Pks_J)
ARABIDOPSIS
cDNA
388
600




Arabidopsis




thaliana Kondara


LNU181
pKS (Pks_J)
ARABIDOPSIS
cDNA
389
761




Arabidopsis




thaliana Kondara


LNU182
pKS (Pks_J)
ARABIDOPSIS
cDNA
390
602




Arabidopsis




thaliana ND


LNU183
pKS (Pks_J)
ARABIDOPSIS
cDNA
391
603




Arabidopsis




thaliana Kondara


LNU184
pKS (Pks_J)
ARABIDOPSIS
cDNA
392
604




Arabidopsis




thaliana ND


LNU185
pKS (Pks_J)
ARABIDOPSIS
cDNA
393
605




Arabidopsis




thaliana Kondara


LNU186
pKS (Pks_J)
ARABIDOPSIS
cDNA
394
606




Arabidopsis




thaliana Kondara


LNU187
pKS (Pks_J)
ARABIDOPSIS
cDNA
395
762




Arabidopsis




thaliana Kondara


LNU188
pKS (Pks_J)
SOYBEAN
cDNA
396
608




Glycine max 58-




261


LNU189
pKS (Pks_J)
RICE Oryza
cDNA
397
609




sativa L. Japonica




ND


LNU19
pGXNa
RICE Oryza
cDNA
274
484




sativa L. Japonica




Nipponbare


LNU190


GeneArt
398
610


LNU191


GeneArt
399
611


LNU192


GeneArt
400
612


LNU196
pKS (Pks_J)
RICE Oryza
cDNA
401
613




sativa L. Japonica




Nipponbare


LNU198


GeneArt
402
614


LNU2
pKS (Pks_J)
RICE Oryza
cDNA
259
469




sativa L. Japonica




LEMONT


LNU20
pGXN
TOMATO
cDNA
275
485



(pKG + Nos + 35S)
Lycopersicum




esculentum M82


LNU200
pGXNa
TOMATO
cDNA
403
615




Lycopersicum




esculentum M82


LNU201


GeneArt
215
—


LNU206


GeneArt
404
617


LNU207
pKS (Pks_J)
ARABIDOPSIS
cDNA
405
618




Arabidopsis




thaliana Kondara


LNU210
pKS (Pks_J)
ARABIDOPSIS
cDNA
406
763




Arabidopsis




thaliana Kondara


LNU211
pGXN
ARABIDOPSIS
cDNA
407
620



(pKG + Nos + 35S)
Arabidopsis




thaliana Kondara


LNU212
pGXN
ARABIDOPSIS
cDNA
408
764



(pKG + Nos + 35S)
Arabidopsis




thaliana Kondara


LNU213
pKS (Pks_J)
ARABIDOPSIS
cDNA
409
622




Arabidopsis




thaliana Columbia




wt


LNU214
pKS (Pks_J)
ARABIDOPSIS
cDNA
410
765




Arabidopsis




thaliana Kondara


LNU215
pKS (Pks_J)
ARABIDOPSIS
cDNA
411
624




Arabidopsis




thaliana Kondara


LNU216
pKS (Pks_J)
RICE Oryza
cDNA
412
625




sativa L. Japonica




LEMONT


LNU217
pKS (Pks_J)
RICE Oryza
cDNA
413
626




sativa L. Indica




TEBBONET


LNU218
pGXN
ARABIDOPSIS
cDNA
414
627



(pKG + Nos + 35S)
Arabidopsis




thaliana Kondara


LNU219
pKS (Pks_J)
ARABIDOPSIS
cDNA
415
766




Arabidopsis




thaliana Kondara


LNU220
pGXN
RICE Oryza
cDNA
416
767



(pKG + Nos + 35S)
sativa L. Japonica




LEMONT


LNU222_H6


GeneArt
465
680


LNU223
pKS (Pks_J)
RICE Oryza
cDNA
417
631




sativa L. Japonica




Nipponbare


LNU224
pKS (Pks_J)
BARLEY
cDNA
418
632




Hordeum vulgare




L. ND


LNU225
pKS (Pks_J)
ARABIDOPSIS
cDNA
419
768




Arabidopsis




thaliana Kondara


LNU228
pGXN
BARLEY
cDNA
420
634



(pKG + Nos + 35S)
Hordeum vulgare




L. Manit


LNU229
pGXN
TOMATO
cDNA
421
635



(pKG + Nos + 35S)
Lycopersicum




esculentum M82


LNU23
pKS (Pks_J)
ARABIDOPSIS
cDNA
276
486




Arabidopsis




thaliana Columbia




wt


LNU230
pKS (Pks_J)
RICE Oryza
cDNA
422
636




sativa L. Japonica




LEMONT


LNU232
Topo B
RICE Oryza
cDNA
423
769




sativa L. Japonica




LEMONT


LNU233
pGXN
RICE Oryza
Genomic
467
—



(pKG + Nos + 35S)
sativa L. Japonica




ND


LNU234
pKS (Pks_J)
ARABIDOPSIS
cDNA
424
638




Arabidopsis




thaliana Kondara


LNU235
pKS (Pks_J)
ARABIDOPSIS
cDNA
425
770




Arabidopsis




thaliana Kondara


LNU236
pKS (Pks_J)
COTTON
cDNA
426
771




Gossypium




hirsutum Akala


LNU239
pKS (Pks_J)
RICE Oryza
cDNA
427
641




sativa L. Japonica




Nipponbare


LNU24
pKS (Pks_J)
ARABIDOPSIS
cDNA
277
487




Arabidopsis




thaliana Kondara


LNU241
pGXN
RICE Oryza
cDNA
428
643



(pKG + Nos + 35S)
sativa L. Japonica




LEMONT


LNU242
pKS (Pks_J)
ARABIDOPSIS
cDNA
429
644




Arabidopsis




thaliana Columbia




wt


LNU243
pGXN
BARLEY
cDNA
430
645



(pKG + Nos + 35S)
Hordeum vulgare




L. Manit


LNU244
pKS (Pks_J)
BARLEY
cDNA
431
772




Hordeum vulgare




L. Manit


LNU245
pGXNa
TOMATO
cDNA
432
773




Lycopersicum




esculentum M82


LNU246
pKS (Pks_J)
TOMATO
cDNA
433
648




Lycopersicum




esculentum M82


LNU247
Topo B
ARABIDOPSIS
cDNA
434
774




Arabidopsis




thaliana Kondara


LNU249
pGXN
ARABIDOPSIS
cDNA
435
650



(pKG + Nos + 35S)
Arabidopsis




thaliana Columbia




wt


LNU25
pKS (Pks_J)
SORGHUM
cDNA
278
488




Sorghum bicolor




ND


LNU250
pKS (Pks_J)
ARABIDOPSIS
cDNA
436
775




Arabidopsis




thaliana Kondara


LNU251
pKS (Pks_J)
ARABIDOPSIS
cDNA
437
776




Arabidopsis




thaliana Kondara


LNU253
pKS (Pks_J)
SOYBEAN
cDNA
438
777




Glycine max 58-




261


LNU254
pKS (Pks_J)
ARABIDOPSIS
cDNA
439
654




Arabidopsis




thaliana Kondara


LNU255
pKS (Pks_J)
ARABIDOPSIS
cDNA
440
778




Arabidopsis




thaliana Kondara


LNU256
pKS (Pks_J)
ARABIDOPSIS
cDNA
441
779




Arabidopsis




thaliana Kondara


LNU257
pGXN
ARABIDOPSIS
cDNA
442
657



(pKG + Nos + 35S)
Arabidopsis




thaliana Kondara


LNU258
pKS (Pks_J)
ARABIDOPSIS
cDNA
443
780




Arabidopsis




thaliana Kondara


LNU260
pKS (Pks_J)
ARABIDOPSIS
cDNA
444
781




Arabidopsis




thaliana Kondara


LNU261
pKS (Pks_J)
ARABIDOPSIS
cDNA
445
782




Arabidopsis




thaliana Kondara


LNU262


GeneArt
446
661


LNU263
pGXN
BARLEY
cDNA
447
662



(pKG + Nos + 35S)
Hordeum vulgare




L. Manit


LNU265


GeneArt
448
663


LNU266
pKS (Pks_J)
MAIZE Zea mays
cDNA
449
783




L. OH43


LNU267
pGXN
MAIZE Zea mays
cDNA
450
665



(pKG + Nos + 35S)
L. B73


LNU268
pKS (Pks_J)
MAIZE Zea mays
cDNA
451
666




L. OH43


LNU27
pGXN
BARLEY
cDNA
279
489



(pKG + Nos + 35S)
Hordeum vulgare




L. Manit


LNU271
pKS (Pks_J)
RICE Oryza
cDNA
452
667




sativa L. Indica




TEBBONET


LNU274
pGXN
RICE Oryza
cDNA
453
668



(pKG + Nos + 35S)
sativa L. Japonica




LEMONT


LNU275


GeneArt
454
669


LNU276
pKS (Pks_J)
RICE Oryza
cDNA
455
784




sativa L. Indica




TEBBONET


LNU277
pKS (Pks_J)
RICE Oryza
cDNA
456
671




sativa L. Japonica




ND


LNU278
Topo B
SORGHUM
cDNA
457
672




Sorghum bicolor




ND


LNU279
pGXN
SORGHUM
cDNA
458
673



(pKG + Nos + 35S)
Sorghum bicolor




ND


LNU28
pGXN
BARLEY
cDNA
280
712



(pKG + Nos + 35S)
Hordeum vulgare




L. Manit


LNU280
pKS (Pks_J)
SORGHUM
cDNA
459
674




Sorghum bicolor




ND


LNU282


GeneArt
460
675


LNU284
pKS (Pks_J)
SOYBEAN
cDNA
461
676




Glycine max 40-




219


LNU287
pKS (Pks_J)
SOYBEAN
cDNA
462
677




Glycine max 40-




219


LNU288
pKS (Pks_J)
TOMATO
cDNA
463
678




Lycopersicum




esculentum M82


LNU289
pKS (Pks_J)
TOMATO
cDNA
464
679




Lycopersicum




esculentum M82


LNU29
pKS (Pks_J)
RICE Oryza
cDNA
281
491




sativa L. Japonica




Nipponbare


LNU3
pGXN
RICE Oryza
cDNA
260
470



(pKG + Nos + 35S)
sativa L. Japonica




LEMONT


LNU32


GeneArt
282
492


LNU33
pKS (Pks_J)
SOYBEAN
cDNA
283
713




Glycine max 58-




261


LNU35
Topo B
WHEAT Triticum
cDNA
284
714




aestivum L. ND


LNU36
pKS (Pks_J)
SOYBEAN
cDNA
285
715




Glycine max 58-




261


LNU37
pKS (Pks_J)
RICE Oryza
cDNA
286
497




sativa L. Japonica




LEMONT


LNU4
pKS (Pks_J)
BARLEY
cDNA
261
708




Hordeum vulgare




L. Manit


LNU40
pGXN
RICE Oryza
cDNA
287
498



(pKG + Nos + 35S)
sativa L. Japonica




PECOS + RICE




Oryza sativa L.




Japonica




LEMONT


LNU43
pKS (Pks_J)
SOYBEAN
cDNA
288
499




Glycine max 58-




261


LNU44
pGXN
SOYBEAN
cDNA
289
500



(pKG + Nos + 35S)
Glycine max 58-




261


LNU45
pGXN
SOYBEAN
cDNA
290
501



(pKG + Nos + 35S)
Glycine max 58-




261


LNU46
pKS (Pks_J)
SOYBEAN
cDNA
291
502




Glycine max 58-




261


LNU48
pKS (Pks_J)
RICE Oryza
cDNA
292
503




sativa L. Japonica




Nipponbare


LNU5
pKS (Pks_J)
BARLEY
cDNA
262
472




Hordeum vulgare




L. ND


LNU50
pGXN
RICE Oryza
cDNA
293
504



(pKG + Nos + 35S)
sativa L. Japonica




ND


LNU51


GeneArt
294
505


LNU52
pKS (Pks_J)
RICE Oryza
cDNA
295
506




sativa L. Japonica




ND


LNU53
pKS (Pks_J)
SOYBEAN
cDNA
296
716




Glycine max 58-




261


LNU54
pGXN
SOYBEAN
cDNA
297
717



(pKG + Nos + 35S)
Glycine max 58-




261


LNU55
pKS (Pks_J)
SOYBEAN
cDNA
298
718




Glycine max 58-




261


LNU56
pGXN
SOYBEAN
cDNA
299
510



(pKG + Nos + 35S)
Glycine max 40-




219


LNU57
pGXN
WHEAT Triticum
cDNA
300
511



(pKG + Nos + 35S)
aestivum L. ND


LNU58
Topo B
WHEAT Triticum
cDNA
301
719




aestivum L. ND


LNU59


GeneArt
302
513


LNU6
pKS (Pks_J)
SOYBEAN
cDNA
263
473




Glycine max 58-




261


LNU60
Topo B
WHEAT Triticum
cDNA
303
514




aestivum L. ND


LNU61
Topo B
WHEAT Triticum
cDNA
304
720




aestivum L. ND


LNU63
pGXN
WHEAT Triticum
cDNA
305
516



(pKG + Nos + 35S)
aestivum L. ND


LNU64
pKS (Pks_J)
WHEAT Triticum
cDNA
306
721




aestivum L. ND


LNU65
pKS (Pks_J)
RICE Oryza
cDNA
307
722




sativa L. Japonica




ND


LNU67
Topo B
RICE Oryza
cDNA
308
723




sativa L. Japonica




ND


LNU68
pKS (Pks_J)
RICE Oryza
cDNA
309
520




sativa L. Japonica




LEMONT


LNU69
pKS (Pks_J)
RICE Oryza
cDNA
310
521




sativa L. Japonica




LEMONT


LNU7
pGXN
SOYBEAN
cDNA
264
474



(pKG + Nos + 35S)
Glycine max 40-




219


LNU70
pGXN
RICE Oryza
cDNA
311
724



(pKG + Nos + 35S)
sativa L. Japonica




LEMONT


LNU71
pKS (Pks_J)
RICE Oryza
cDNA
312
523




sativa L. Japonica




Nipponbare


LNU72
pKS (Pks_J)
BARLEY
cDNA
313
725




Hordeum vulgare




L. Manit


LNU73
pGXNa
RICE Oryza
cDNA
314
525




sativa L. Indica




TEBBONET


LNU74
pGXN
POPLAR Populus
cDNA
315
726



(pKG + Nos + 35S)
ND ND


LNU75


GeneArt
316
527


LNU76
pKS (Pks_J)
RICE Oryza
cDNA
317
528




sativa L. Indica




TEBBONET


LNU79
pKS (Pks_J)
COTTON
cDNA
318
727




Gossypium




hirsutum ND


LNU8
pGXN
ARABIDOPSIS
cDNA
265
475



(pKG + Nos + 35S)
Arabidopsis




thaliana Columbia




wt


LNU81
pGXN
BARLEY
cDNA
319
728



(pKG + Nos + 35S)
Hordeum vulgare




L. Manit


LNU82
pKS (Pks_J)
RICE Oryza
cDNA
320
531




sativa L. Japonica




LEMONT


LNU83
pKS (Pks_J)
SOYBEAN
cDNA
321
532




Glycine max 58-




261


LNU84
pKS (Pks_J)
SORGHUM
cDNA
322
729




Sorghum bicolor




ND


LNU85
pGXN
SORGHUM
cDNA
323
534



(pKG + Nos + 35S)
Sorghum bicolor




ND


LNU86
pKS (Pks_J)
MAIZE Zea mays
cDNA
324
730




L. OH43


LNU87
pGXN
SORGHUM
cDNA
325
731



(pKG + Nos + 35S)
Sorghum bicolor




ND


LNU89
pKS (Pks_J)
WHEAT Triticum
cDNA
326
732




aestivum L.


LNU9
pGXN
RICE Oryza
cDNA
266
476



(pKG + Nos + 35S)
sativa L. Japonica




ND


LNU94
pGXN
TOMATO
cDNA
327
538



(pKG + Nos + 35S)
Lycopersicum




esculentum M82


LNU95
pKS (Pks_J)
SOYBEAN
cDNA
328
539




Glycine max 58-




261


LNU96
pGXN
RICE Oryza
cDNA
329
733



(pKG + Nos + 35S)
sativa L. Japonica




LEMONT


LNU98
pKS (Pks_J)
MAIZE Zea mays
cDNA
330
734




L. B73





Table 59.


“Polyn.”—Polynucleotide;


“Polyp.”—polypeptide.






Example 12
Transforming Agrobacterium tumefaciens Cells with Binary Vectors Harboring Putative Genes
Each of the binary vectors described in Example 11 above were used to transform Agrobacterium cells. Two additional binary constructs, having only the At6669, or the RootP promoter or no additional promoter were used as negative controls.
The binary vectors were introduced to Agrobacterium tumefaciens GV301, or LB4404 competent cells (about 109 cells/mL) by electroporation. The electroporation was performed using a MicroPulser electroporator (Biorad), 0.2 cm cuvettes (Biorad) and EC-2 electroporation program (Biorad). The treated cells were cultured in LB liquid medium at 28° C. for 3 hours, then plated over LB agar supplemented with gentamycin (50 mg/L; for Agrobacterium strains GV301) or streptomycin (300 mg/L; for Agrobacterium strain LB4404) and kanamycin (50 mg/L) at 28° C. for 48 hours. Agrobacterium colonies, which were developed on the selective media, were further analyzed by PCR using the primers designed to span the inserted sequence in the pPI plasmid. The resulting PCR products were isolated and sequenced as described in Example 11 above, to verify that the correct polynucleotide sequences of the invention are properly introduced to the Agrobacterium cells.
Example 13
Transformation of Arabidopsis thaliana Plants with the Polynucleotides of the Invention
Arabidopsis thaliana Columbia plants (T0 plants) were transformed using the Floral Dip procedure described by Clough and Bent, 1998 (Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J 16:735-43) and by Desfeux et al., 2000 (Female Reproductive Tissues Are the Primary Target of Agrobacterium-Mediated Transformation by the Arabidopsis Floral-Dip Method. Plant Physiol, July 2000, Vol. 123, pp. 895-904), with minor modifications. Briefly, T0 Plants were sown in 250 ml pots filled with wet peat-based growth mix. The pots are covered with aluminum foil and a plastic dome, kept at 4° C. for 3-4 days, then uncovered and incubated in a growth chamber at 18-24° C. under 16/8 hour light/dark cycles. The T0 plants were ready for transformation six days before anthesis.
Single colonies of Agrobacterium carrying the binary constructs, were generated as described in Examples 11 and 12 above. Colonies were cultured in LB medium supplemented with kanamycin (50 mg/L) and gentamycin (50 mg/L). The cultures were incubated at 28° C. for 48 hours under vigorous shaking and then centrifuged at 4000 rpm for 5 minutes. The pellets comprising the Agrobacterium cells were re-suspended in a transformation medium containing half-strength (2.15 g/L) Murashige-Skoog (Duchefa); 0.044 μM benzylamino purine (Sigma); 112 μg/L B5 Gambourg vitamins (Sigma); 5% sucrose; and 0.2 ml/L Silwet L-77 (OSI Specialists, CT) in double-distilled water, at pH of 5.7.
Transformation of T0 plants was performed by inverting each plant into an Agrobacterium suspension, such that the above ground plant tissue was submerged for 3-5 seconds. Each inoculated T0 plant was immediately placed in a plastic tray, then covered with clear plastic dome to maintain humidity and was kept in the dark at room temperature for 18 hours, to facilitate infection and transformation. Transformed (transgenic) plants were then uncovered and transferred to a greenhouse for recovery and maturation. The transgenic T0 plants were grown in the greenhouse for 3-5 weeks until siliques are brown and dry. Seeds were harvested from plants and kept at room temperature until sowing.
For generating T1 and T2 transgenic plants harboring the genes, seeds collected from transgenic T0 plants were surface-sterilized by soaking in 70% ethanol for 1 minute, followed by soaking in 5% sodium hypochloride and 0.05% triton for 5 minutes. The surface-sterilized seeds were thoroughly washed in sterile distilled water then placed on culture plates containing half-strength Murashige-Skoog (Duchefa); 2% sucrose; 0.8% plant agar; 50 mM kanamycin; and 200 mM carbenicylin (Duchefa). The culture plates were incubated at 4° C. for 48 hours, then transferred to a growth room at 25° C. for an additional week of incubation. Vital T1 Arabidopsis plants were transferred to fresh culture plates for another week of incubation. Following incubation the T1 plants were removed from culture plates and planted in growth mix contained in 250 ml pots. The transgenic plants were allowed to grow in a greenhouse to maturity. Seeds harvested from T1 plants were cultured and grown to maturity as T2 plants under the same conditions as used for culturing and growing the T1 plants.
Example 14
Evaluating Transgenic Arabidopsis NUE Under Low or Normal Nitrogen Conditions Using In Vitro (Tissue Culture) Assays
Assay 1: Plant Growth Under Low and Favorable Nitrogen Concentration Levels
Surface sterilized seeds were sown in basal media [50% Murashige-Skoog medium (MS) supplemented with 0.8% plant agar as solidifying agent] in the presence of Kanamycin (used as a selecting agent). After sowing, plates were transferred for 2-3 days for stratification at 4° C. and then grown at 25° C. under 12-hour light 12-hour dark daily cycles for 7 to 10 days. At this time point, seedlings randomly chosen were carefully transferred to plates containing 1⁄2 MS media (15 mM N) for the normal nitrogen concentration treatment and 0.75 mM nitrogen for the low nitrogen concentration treatments. For experiments performed in T2 lines, each plate contained 5 seedlings of the same transgenic event, and 3-4 different plates (replicates) for each event. For each polynucleotide of the invention at least four-five independent transformation events were analyzed from each construct. For experiments performed in T1 lines, each plate contained 5 seedlings of 5 independent transgenic events and 3-4 different plates (replicates) were planted. In total, for T1 lines, 20 independent events were evaluated. Plants expressing the polynucleotides of the invention were compared to the average measurement of the control plants (empty vector or GUS reporter gene under the same promoter) used in the same experiment.
Digital imaging—A laboratory image acquisition system, which consists of a digital reflex camera (Canon EOS 300D) attached with a 55 mm focal length lens (Canon EF-S series), mounted on a reproduction device (Kaiser RS), which includes 4 light units (4×150 Watts light bulb) and located in a darkroom, is used for capturing images of plantlets sawn in agar plates.
The image capturing process is repeated every 3-4 days starting at day 1 till day 10 (see for example the images in FIGS. 3A-B). An image analysis system was used, which consists of a personal desktop computer (Intel P4 3.0 GHz processor) and a public domain program—ImageJ 1.39 [Java based image processing program which was developed at the U.S. National Institutes of Health and freely available on the internet at Hypertext Transfer Protocol://rsbweb (dot) nih (dot) gov/]. Images were captured in resolution of 10 Mega Pixels (3888×2592 pixels) and stored in a low compression JPEG (Joint Photographic Experts Group standard) format. Next, analyzed data was saved to text files and processed using the JMP statistical analysis software (SAS institute).
Seedling analysis—Using the digital analysis seedling data was calculated, including leaf area, root coverage and root length.
The relative growth rate for the various seedling parameters was calculated according to the following formulas XIII, V (described above) and XIV.

Relative growth rate of leaf area=Regression coefficient of leaf area along time course.  Formula XIII:

Relative growth rate of root length=Regression coefficient of root length along time course.  Formula XIV:

At the end of the experiment, plantlets were removed from the media and weighed for the determination of plant fresh weight. Plantlets were then dried for 24 hours at 60° C., and weighed again to measure plant dry weight for later statistical analysis. Growth rate was determined by comparing the leaf area coverage, root coverage and root length, between each couple of sequential photographs, and results are used to resolve the effect of the gene introduced on plant vigor under optimal conditions. Similarly, the effect of the gene introduced on biomass accumulation, under optimal conditions, was determined by comparing the plants' fresh and dry weight to that of control plants (containing an empty vector or the GUS reporter gene under the same promoter). From every construct created, 3-5 independent transformation events are examined in replicates.
Statistical analyses—To identify genes conferring significantly improved plant vigor or enlarged root architecture, the results obtained from the transgenic plants were compared to those obtained from control plants. To identify outperforming genes and constructs, results from the independent transformation events tested were analyzed separately. To evaluate the effect of a gene event over a control the data was analyzed by Student's t-test and the p value is calculated. Results were considered significant if p<0.1. The IMP statistics software package was used (Version 5.2.1, SAS Institute Inc., Cary, N.C., USA).
Experimental Results:
The genes presented in Tables 60-63 showed a significant improvement in plant NUE since they produced larger plant biomass (plant fresh and dry weight and leaf area) in T2 generation (Tables 60-61) or T1 generation (Tables 62-63) when grown under limiting nitrogen growth conditions, compared to control plants. The genes were cloned under the regulation of a constitutive promoter (At6669) or root preferred promoter (RootP). The evaluation of each gene was carried out by testing the performance of different number of events. Some of the genes were evaluated in more than one tissue culture assay. The results obtained in these second experiments were significantly positive as well.







TABLE 60







Genes showing improved plant performance at nitrogen deficient conditions (T2 generation)











Plant Biomass Fresh

Plant Biomass Dry



Weight [gr.]

Weight [gr.]
















Gene


p-
%
Gene


p-
%


Name
Event #
Ave.
value
incr.
Name
Event #
Ave.
value
incr.



















CONT.
—
0.125
—
0.0
CONT.
—
0.005
—
0.0


LNU100
14474.3
0.198
0.046
58.5
LNU100
14474.3
0.009
0.023
74.5


LNU100
14473.1
0.145
0.099
15.8
LNU100
14473.1
0.006
0.196
16.7


LNU100
14473.3
0.132
0.728
5.7
LNU104
25033.3
0.007
0.059
40.2


LNU104
25033.3
0.166
0.090
32.5
LNU104
25034.1
0.006
0.310
17.2


LNU104
25034.1
0.130
0.761
3.9
LNU213
24654.4
0.010
0.079
97.5


LNU213
24654.4
0.228
0.068
82.1
LNU213
24653.2
0.009
0.000
77.0


LNU213
24653.2
0.183
0.007
46.2
LNU213
24651.1
0.006
0.454
10.3


LNU213
24651.1
0.134
0.538
7.6
LNU218
24781.7
0.008
0.221
48.0


LNU218
24781.7
0.169
0.355
35.0
LNU218
24783.2
0.007
0.041
44.1


LNU218
24783.2
0.160
0.034
28.3
LNU4
25134.2
0.008
0.017
59.3


LNU4
25134.1
0.186
0.009
49.2
LNU4
25134.1
0.008
0.013
50.0


LNU4
25134.2
0.181
0.051
45.1
LNU48
24802.2
0.010
0.026
104.4


LNU48
24802.2
0.223
0.017
78.4
LNU48
24804.4
0.007
0.014
42.6


LNU48
24803.2
0.151
0.082
20.9
LNU48
24803.2
0.007
0.036
40.2


LNU48
24804.4
0.146
0.090
16.5
LNU8
25063.1
0.011
0.040
118.6


LNU8
25063.1
0.244
0.026
95.6
LNU8
25063.6
0.009
0.175
76.5


LNU8
25063.6
0.182
0.150
45.4
LNU8
25062.2
0.005
0.689
6.9


LNU94
24833.3
0.167
0.027
34.0
LNU94
24833.3
0.008
0.001
47.5


LNU94
24834.1
0.160
0.030
27.7
LNU94
24834.1
0.007
0.008
46.6


LNU94
24834.4
0.133
0.660
6.7
LNU94
24834.4
0.006
0.359
15.7


CONT.
—
0.110
—
0.0
CONT.
—
0.005
—
0.0


LNU1
24681.3
0.178
0.041
60.9
LNU1
24682.2
0.008
0.015
64.4


LNU1
24682.2
0.155
0.069
40.8
LNU1
24681.3
0.008
0.018
53.7


LNU1
24684.1
0.141
0.037
28.1
LNU1
24681.1
0.007
0.062
29.3


LNU1
24681.1
0.130
0.167
17.9
LNU1
24684.1
0.007
0.036
28.3


LNU1
24682.1
0.120
0.640
8.5
LNU1
24682.1
0.006
0.279
21.5


LNU133
24744.3
0.178
0.005
61.2
LNU133
24741.1
0.009
0.022
79.5


LNU133
24741.1
0.174
0.020
57.2
LNU133
24744.3
0.009
0.001
79.0


LNU133
24741.2
0.117
0.614
5.5
LNU133
24741.2
0.006
0.105
21.5


LNU133
24744.2
0.115
0.651
4.5
LNU133
24744.2
0.006
0.322
11.7


LNU175
24732.4
0.220
0.076
99.3
LNU175
24732.4
0.012
0.054
127.3


LNU175
24732.1
0.191
0.001
72.7
LNU175
24732.1
0.010
0.006
100.0


LNU175
24734.4
0.153
0.155
38.4
LNU175
24734.4
0.008
0.135
52.7


LNU178
14611.5
0.167
0.005
50.8
LNU175
24731.2
0.005
0.566
6.3


LNU178
14614.5
0.141
0.050
27.5
LNU178
14611.5
0.009
0.002
70.7


LNU178
14612.1
0.119
0.527
8.2
LNU178
14614.5
0.007
0.031
38.0


LNU178
14611.1
0.114
0.751
3.3
LNU178
14611.1
0.006
0.448
10.7


LNU215
24664.3
0.174
0.046
57.8
LNU215
24664.3
0.008
0.012
54.1


LNU215
24661.4
0.115
0.781
4.0
LNU215
24661.4
0.006
0.592
9.8


LNU24
24971.2
0.173
0.026
56.6
LNU215
24664.2
0.006
0.567
7.3


LNU24
24973.1
0.164
0.014
48.2
LNU24
24973.1
0.009
0.030
67.8


LNU24
24971.4
0.140
0.317
26.8
LNU24
24971.2
0.008
0.044
63.9


LNU6
24992.3
0.176
0.003
59.8
LNU24
24971.4
0.008
0.193
46.3


LNU6
24994.1
0.137
0.085
24.0
LNU24
24972.1
0.006
0.307
11.2


LNU6
24994.2
0.128
0.208
16.3
LNU6
24992.3
0.009
0.013
67.8


LNU6
24993.3
0.122
0.581
10.2
LNU6
24994.2
0.007
0.040
35.1


LNU6
24994.5
0.118
0.554
6.8
LNU6
24994.1
0.006
0.081
25.4


LNU82
24823.1
0.133
0.171
20.5
LNU6
24993.3
0.006
0.434
12.7


LNU9
25001.3
0.155
0.200
40.5
LNU6
24994.5
0.006
0.322
12.7


LNU9
25001.1
0.138
0.268
25.4
LNU82
24823.1
0.006
0.086
24.4


LNU9
25003.1
0.123
0.419
11.3
LNU9
25001.3
0.007
0.102
33.7


CONT.
—
0.104
—
0.0
LNU9
25001.1
0.007
0.162
33.2


LNU120
25463.7
0.172
0.047
66.1
LNU9
25003.1
0.006
0.360
19.0


LNU120
25463.3
0.151
0.018
45.3
CONT.
—
0.005
—
0.0


LNU124
14501.7
0.179
0.001
72.5
LNU120
25463.7
0.007
0.230
48.7


LNU124
14502.7
0.120
0.105
15.5
LNU120
25463.3
0.006
0.025
21.5


LNU124
14501.1
0.117
0.229
12.9
LNU124
14501.7
0.007
0.018
41.5


LNU132
14101.9
0.131
0.316
26.1
LNU132
14101.9
0.005
0.616
7.7


LNU132
14102.9
0.126
0.145
21.4
LNU140
14112.7
0.010
0.012
110.3


LNU140
14112.7
0.198
0.000
90.9
LNU180
24724.3
0.012
0.000
148.2


LNU140
14111.6
0.120
0.473
16.0
LNU180
24723.3
0.006
0.044
32.3


LNU140
14114.8
0.118
0.251
13.9
LNU20
24932.4
0.005
0.414
10.8


LNU140
14112.6
0.114
0.467
9.9
LNU36
25562.3
0.009
0.130
82.6


LNU180
24724.3
0.272
0.000
162.1
LNU71
25853.4
0.007
0.043
53.3


LNU180
24723.3
0.201
0.010
93.9
CONT.
—
0.005
—
0.0


LNU180
24722.2
0.110
0.700
6.2
LNU1
24681.3
0.007
0.295
45.1


LNU196
25534.1
0.138
0.168
32.7
LNU1
24683.2
0.005
0.703
4.9


LNU196
25533.1
0.119
0.207
15.0
LNU110
24952.2
0.015
0.450
215.1


LNU20
24932.4
0.149
0.119
43.4
LNU110
24952.3
0.008
0.159
60.0


LNU20
24933.2
0.112
0.591
7.4
LNU110
24953.3
0.006
0.466
18.6


LNU36
25562.3
0.193
0.138
85.9
LNU110
24953.2
0.005
0.276
13.3


LNU36
25562.4
0.115
0.538
10.9
LNU175
24732.2
0.008
0.127
66.8


LNU71
25853.4
0.187
0.035
79.9
LNU175
24733.4
0.005
0.748
5.9


LNU71
25852.4
0.117
0.413
13.2
LNU19
25151.1
0.006
0.094
25.0


CONT.
—
0.117
—
0.0
LNU19
25153.3
0.006
0.456
24.5


LNU1
24681.3
0.156
0.381
32.5
LNU215
24664.2
0.008
0.001
70.0


LNU1
24683.2
0.133
0.365
13.5
LNU215
24663.3
0.005
0.272
12.8


LNU110
24953.3
0.164
0.014
40.1
LNU215
24663.4
0.005
0.339
10.7


LNU110
24952.3
0.164
0.248
39.7
LNU27
24873.4
0.007
0.097
46.7


LNU110
24953.2
0.130
0.389
11.1
LNU27
24873.1
0.007
0.161
45.7


LNU175
24732.2
0.182
0.105
55.4
LNU27
24872.4
0.005
0.541
6.5


LNU175
24733.4
0.139
0.069
18.7
LNU44
24924.2
0.019
0.270
304.1


LNU175
24734.4
0.128
0.430
9.5
LNU44
24924.3
0.009
0.055
100.2


LNU19
25151.1
0.186
0.081
58.6
LNU44
24922.3
0.005
0.770
8.1


LNU19
25153.3
0.155
0.241
32.5
LNU44
24923.3
0.005
0.576
5.4


LNU19
25151.11
0.129
0.581
10.0
LNU54
24903.5
0.012
0.004
154.8


LNU215
24664.2
0.196
0.020
67.1
LNU54
24903.3
0.008
0.014
79.0


LNU215
24663.4
0.147
0.139
25.4
LNU54
24901.2
0.008
0.049
75.3


LNU215
24663.3
0.128
0.466
8.9
LNU79
24884.4
0.011
0.028
122.5


LNU215
24661.4
0.127
0.595
8.4
LNU79
24884.3
0.008
0.050
72.1


LNU27
24873.1
0.205
0.097
74.4
LNU79
24881.1
0.007
0.041
47.8


LNU27
24873.4
0.172
0.044
46.2
LNU79
24882.2
0.005
0.321
13.3


LNU27
24872.4
0.130
0.200
10.6
CONT.
—
0.006
—
0.0


LNU44
24924.3
0.239
0.019
103.9
LNU109
24891.5
0.009
0.009
52.1


LNU44
24924.2
0.218
0.056
85.8
LNU109
24892.6
0.008
0.184
38.3


LNU44
24922.3
0.151
0.203
28.6
LNU109
24892.6
0.008
0.164
29.3


LNU44
24923.3
0.123
0.759
4.5
LNU109
24891.2
0.007
0.371
28.0


LNU54
24903.5
0.259
0.000
121.0
LNU110
24952.1
0.015
0.000
162.5


LNU54
24901.2
0.201
0.005
70.9
LNU110
24953.2
0.010
0.036
79.1


LNU54
24903.3
0.166
0.071
41.4
LNU110
24952.3
0.009
0.153
51.6


LNU79
24884.4
0.247
0.003
110.9
LNU110
24954.3
0.007
0.063
27.1


LNU79
24884.3
0.190
0.031
61.6
LNU133
24744.3
0.011
0.020
90.7


LNU79
24881.1
0.169
0.074
44.2
LNU133
24741.1
0.010
0.091
63.2


LNU79
24882.2
0.135
0.113
14.9
LNU133
24741.2
0.008
0.006
37.5


CONT.
—
0.138
—
0.0
LNU133
24744.2
0.007
0.220
21.1


LNU109
24892.5
0.200
0.181
44.6
LNU133
24742.2
0.006
0.581
5.7


LNU109
24891.5
0.179
0.056
29.3
LNU19
25151.1
0.011
0.124
86.9


LNU109
24891.2
0.179
0.351
29.1
LNU27
24873.4
0.012
0.015
107.9


LNU109
24892.6
0.160
0.417
15.9
LNU44
24922.3
0.014
0.009
136.3


LNU110
24952.1
0.333
0.006
140.4
LNU44
24923.1
0.007
0.129
25.0


LNU110
24953.2
0.224
0.053
61.9
LNU44
24924.3
0.007
0.191
21.6


LNU110
24952.3
0.189
0.257
36.4
LNU54
24901.2
0.009
0.041
59.4


LNU133
24744.3
0.213
0.014
54.0
LNU54
24903.5
0.009
0.036
58.5


LNU133
24741.1
0.176
0.226
27.1
LNU54
24902.4
0.009
0.037
52.9


LNU133
24741.2
0.175
0.056
26.5
LNU6
24994.5
0.009
0.047
55.1


LNU133
24744.2
0.150
0.549
8.5
LNU6
24992.3
0.009
0.018
54.6


LNU19
25151.1
0.198
0.151
42.8
LNU6
24994.1
0.007
0.450
22.0


LNU27
24873.4
0.235
0.021
70.0
LNU6
24994.2
0.007
0.201
14.3


LNU44
24922.3
0.259
0.004
86.9
LNU79
24884.4
0.010
0.008
79.1


LNU44
24923.1
0.162
0.055
17.1
LNU79
24882.2
0.010
0.005
64.5


LNU44
24924.3
0.156
0.511
12.6
LNU79
24881.1
0.008
0.049
45.2


LNU54
24901.2
0.215
0.060
55.2
LNU79
24884.3
0.007
0.204
21.6


LNU54
24903.5
0.188
0.013
36.2
CONT.
—
0.004
—
0.0


LNU54
24902.4
0.187
0.003
35.2
LNU109
24892.8
0.009
0.040
152.1


LNU6
24992.3
0.177
0.118
27.9
LNU109
24891.2
0.009
0.005
140.4


LNU6
24994.5
0.174
0.084
25.8
LNU109
24892.5
0.005
0.058
48.6


LNU6
24994.1
0.165
0.300
19.0
LNU109
24891.5
0.005
0.205
35.6


LNU6
24994.2
0.144
0.779
4.4
LNU143
25971.5
0.006
0.039
65.1


LNU79
24884.4
0.218
0.020
57.8
LNU143
25975.3
0.006
0.183
58.9


LNU79
24882.2
0.206
0.105
48.6
LNU143
25972.1
0.005
0.046
47.9


LNU79
24881.1
0.169
0.118
21.8
LNU143
25975.2
0.005
0.118
41.1


CONT.
—
0.113
—
0.0
LNU143
25971.2
0.004
0.699
6.8


LNU109
24892.8
0.192
0.149
69.7
LNU154
14601.6
0.007
0.067
104.1


LNU109
24891.2
0.178
0.028
57.7
LNU154
14604.7
0.007
0.095
102.7


LNU109
24892.5
0.139
0.350
23.4
LNU154
14604.6
0.007
0.101
80.8


LNU109
24891.5
0.121
0.763
6.9
LNU154
14602.8
0.006
0.092
52.1


LNU143
25972.1
0.143
0.231
26.2
LNU154
14604.4
0.005
0.233
36.3


LNU143
25975.3
0.132
0.524
16.8
LNU196
25532.2
0.010
0.019
164.4


LNU143
25971.5
0.124
0.536
9.9
LNU196
25534.1
0.007
0.075
93.2


LNU154
14604.7
0.167
0.182
47.9
LNU196
25531.2
0.006
0.150
54.8


LNU154
14604.6
0.164
0.116
45.2
LNU196
25532.1
0.005
0.350
38.4


LNU154
14601.6
0.154
0.353
36.3
LNU207
24642.5
0.008
0.086
119.2


LNU196
25532.2
0.220
0.017
94.9
LNU207
24642.4
0.008
0.029
115.1


LNU196
25534.1
0.172
0.103
52.6
LNU207
24644.18
0.007
0.040
78.8


LNU196
25531.2
0.140
0.382
24.3
LNU207
24641.1
0.004
0.564
11.0


LNU196
25532.1
0.121
0.716
7.6
LNU288
14562.12
0.005
0.045
49.3


LNU207
24642.5
0.177
0.042
56.6
LNU288
14562.7
0.005
0.181
45.2


LNU207
24642.4
0.166
0.022
47.3
LNU288
14562.9
0.005
0.292
36.3


LNU207
24644.18
0.152
0.058
34.8
LNU288
14562.1
0.004
0.408
21.9


LNU288
14562.9
0.120
0.720
5.8
LNU288
14564.9
0.004
0.427
17.1


LNU50
26024.2
0.153
0.131
35.4
LNU50
26024.2
0.006
0.085
58.9


LNU52
25723.2
0.242
0.001
114.0
LNU50
26025.4
0.005
0.080
39.0


LNU52
25721.4
0.172
0.302
52.1
LNU50
26023.2
0.005
0.207
37.0


LNU52
25721.3
0.125
0.624
10.3
LNU50
26022.1
0.004
0.464
19.9


CONT.
—
0.125
—
0.0
LNU50
26023.5
0.004
0.507
13.0


LNU143
25975.2
0.132
0.701
6.0
LNU52
25723.2
0.012
0.001
230.8


LNU154
14602.8
0.152
0.202
21.6
LNU52
25721.4
0.009
0.172
134.2


LNU154
14604.4
0.140
0.688
12.4
LNU52
25721.3
0.006
0.062
54.8


LNU154
14601.6
0.133
0.706
6.7
LNU52
25721.1
0.005
0.481
32.2


LNU207
24642.5
0.227
0.038
81.8
CONT.
—
0.006
—
0.0


LNU207
24641.1
0.197
0.068
58.0
LNU143
25975.3
0.006
0.736
5.7


LNU207
24642.4
0.155
0.239
24.6
LNU154
14602.8
0.008
0.061
36.7


LNU211
24771.1
0.143
0.348
14.4
LNU154
14601.6
0.006
0.592
10.2


LNU211
24774.4
0.135
0.764
7.9
LNU207
24642.5
0.011
0.028
91.5


LNU52
25723.2
0.202
0.194
61.8
LNU207
24641.1
0.008
0.094
51.5


LNU52
25721.2
0.191
0.018
52.8
LNU207
24642.4
0.007
0.244
28.1


LNU52
25723.1
0.183
0.022
46.8
LNU211
24771.1
0.009
0.007
60.5


LNU52
25721.1
0.170
0.140
36.2
LNU211
24774.4
0.007
0.411
18.7


LNU69
14572.8
0.192
0.089
54.2
LNU52
25721.2
0.009
0.029
59.2


LNU69
14571.1
0.164
0.335
31.7
LNU52
25721.1
0.009
0.065
56.0


LNU69
14572.9
0.131
0.794
5.2
LNU52
25723.2
0.008
0.176
48.4


CONT.
—
0.123
—
0.0
LNU52
25723.1
0.007
0.241
23.7


LNU150
24842.9
0.199
0.000
61.6
LNU69
14571.1
0.008
0.095
48.4


LNU150
24841.9
0.168
0.204
36.0
LNU69
14572.8
0.006
0.278
14.7


LNU150
24843.5
0.152
0.280
23.5
LNU69
14572.9
0.006
0.482
12.0


LNU179
24632.5
0.185
0.008
49.8
CONT.
—
0.006
—
0.0


LNU179
24631.9
0.146
0.079
18.6
LNU150
24842.9
0.009
0.000
60.6


LNU232
26003.7
0.148
0.137
20.4
LNU150
24841.9
0.007
0.462
19.7


LNU235
26185.3
0.182
0.161
47.4
LNU179
24632.5
0.008
0.139
42.3


LNU235
26184.4
0.173
0.171
40.6
LNU235
26184.4
0.008
0.111
43.6


LNU242
25473.1
0.184
0.064
48.9
LNU235
26185.3
0.007
0.425
21.4


LNU242
25474.1
0.158
0.012
28.1
LNU242
25473.1
0.007
0.047
23.5


LNU242
25471.1
0.151
0.138
22.4
LNU242
25474.1
0.007
0.335
11.2


LNU76
26423.1
0.181
0.032
46.5
LNU242
25471.1
0.006
0.525
8.2


LNU76
26421.2
0.163
0.150
31.9
LNU76
26423.1
0.008
0.048
38.0


LNU76
26425.1
0.148
0.327
20.1
LNU76
26421.2
0.007
0.155
23.5


LNU76
26421.1
0.145
0.294
18.0
LNU76
26425.1
0.007
0.569
12.9


LNU76
26422.2
0.136
0.415
10.4
LNU76
26421.1
0.006
0.730
5.6


LNU95
13985.11
0.211
0.001
71.2
LNU95
13985.11
0.011
0.001
84.9


LNU95
13985.15
0.145
0.495
17.6
LNU95
13985.12
0.007
0.535
13.3


LNU95
13985.12
0.144
0.439
16.7
LNU95
13985.15
0.007
0.716
11.6


CONT.
—
0.128
—
0.0
CONT.
—
0.006
—
0.0


LNU118
14013.8
0.187
0.095
46.0
LNU118
14013.6
0.010
0.019
70.3


LNU118
14013.6
0.182
0.009
41.9
LNU118
14012.15
0.008
0.122
41.6


LNU118
14012.15
0.166
0.282
29.8
LNU118
14013.8
0.008
0.070
38.2


LNU150
24841.9
0.163
0.240
27.0
LNU118
14012.12
0.007
0.604
12.1


LNU150
24842.9
0.136
0.793
6.2
LNU150
24841.6
0.007
0.213
19.8


LNU150
24841.6
0.135
0.634
5.2
LNU150
24841.9
0.007
0.506
13.4


LNU179
24631.7
0.161
0.036
25.9
LNU179
24631.7
0.008
0.031
36.0


LNU179
24631.6
0.148
0.451
15.4
LNU179
24631.6
0.007
0.225
19.4


LNU179
24631.9
0.137
0.728
7.2
LNU232
26001.5
0.008
0.191
41.6


LNU232
26001.5
0.161
0.388
25.6
LNU232
26003.6
0.006
0.660
8.7


LNU235
26185.2
0.241
0.023
88.2
LNU235
26184.4
0.013
0.002
115.2


LNU235
26184.4
0.237
0.006
85.1
LNU235
26185.2
0.012
0.020
99.8


LNU235
26184.2
0.211
0.044
64.6
LNU235
26184.2
0.010
0.019
72.4


LNU235
26182.1
0.147
0.303
14.3
LNU242
25474.1
0.007
0.042
26.6


LNU242
25474.1
0.148
0.306
15.6
LNU288
14563.9
0.011
0.003
83.1


LNU288
14563.9
0.232
0.000
81.1
LNU288
14562.1
0.010
0.004
65.6


LNU288
14562.1
0.208
0.025
62.0
LNU288
14564.9
0.007
0.114
23.6


LNU288
14564.9
0.169
0.117
31.9
LNU288
14562.7
0.007
0.420
15.9


LNU288
14562.7
0.166
0.237
29.2
LNU76
26421.2
0.009
0.307
56.1


LNU76
26421.2
0.193
0.330
50.5
LNU76
26423.1
0.008
0.313
41.6


LNU76
26423.1
0.176
0.314
37.7
LNU76
26422.2
0.007
0.489
18.1


LNU76
26422.2
0.151
0.434
18.1
LNU95
13985.16
0.014
0.005
133.6


LNU95
13985.16
0.250
0.003
94.8
LNU95
13985.12
0.011
0.000
92.9


LNU95
13985.12
0.239
0.001
86.4
LNU95
13985.15
0.011
0.020
86.1


LNU95
13985.15
0.223
0.008
74.1
LNU95
13985.19
0.007
0.490
11.7


LNU95
13985.19
0.136
0.658
6.5
CONT.
—
0.007
—
0.0


CONT.
—
0.149
—
0.0
LNU101
27632.7
0.007
0.435
9.2


LNU101
27632.7
0.179
0.122
19.6
LNU101
27632.1
0.007
0.685
5.9


LNU128
26515.3
0.210
0.062
40.6
LNU101
27635.1
0.007
0.516
4.4


LNU192
28315.2
0.200
0.012
33.7
LNU128
26515.3
0.010
0.081
45.4


LNU206
27621.2
0.180
0.159
20.7
LNU192
28315.2
0.009
0.015
33.5


LNU211
24771.1
0.208
0.018
39.5
LNU192
28313.3
0.007
0.598
4.4


LNU211
24773.2
0.162
0.576
8.8
LNU206
27621.2
0.008
0.001
23.8


LNU282
27563.3
0.204
0.036
36.4
LNU211
24771.1
0.011
0.059
66.6


LNU282
27562.1
0.164
0.466
9.7
LNU211
24773.2
0.008
0.451
22.3


LNU69
14571.1
0.208
0.022
39.5
LNU211
24773.1
0.007
0.509
5.9


LNU75
27572.1
0.163
0.669
8.9
LNU282
27563.3
0.010
0.018
54.7


LNU75
27572.2
0.153
0.779
2.3
LNU282
27563.1
0.008
0.141
17.1


CONT.
—
0.121
—
0.0
LNU282
27562.1
0.007
0.571
7.7


LNU101
27632.5
0.151
0.151
25.2
LNU69
14571.1
0.011
0.000
58.1


LNU118
14013.6
0.194
0.050
60.0
LNU69
14572.9
0.007
0.722
6.2


LNU118
14013.9
0.155
0.605
28.5
LNU75
27572.2
0.008
0.191
18.5


LNU118
14012.15
0.153
0.053
26.6
LNU75
27572.1
0.007
0.647
7.4


LNU118
14012.12
0.128
0.683
5.7
CONT.
—
0.005
—
0.0


LNU206
27621.1
0.208
0.003
72.3
LNU101
27632.5
0.006
0.003
36.7


LNU206
27622.1
0.208
0.033
72.2
LNU118
14013.6
0.007
0.027
59.8


LNU206
27621.2
0.207
0.062
71.3
LNU118
14012.15
0.007
0.008
50.7


LNU206
27622.4
0.162
0.285
34.0
LNU118
14013.8
0.005
0.693
9.9


LNU249
26153.1
0.209
0.002
73.0
LNU118
14013.9
0.005
0.674
6.2


LNU249
26154.2
0.168
0.104
39.1
LNU206
27621.1
0.010
0.003
112.9


LNU282
27563.1
0.205
0.000
69.2
LNU206
27622.1
0.010
0.056
110.7


LNU282
27565.2
0.172
0.081
42.2
LNU206
27621.2
0.010
0.073
109.1


LNU282
27565.1
0.136
0.457
12.7
LNU206
27622.4
0.007
0.230
57.6


LNU288
14564.8
0.255
0.004
110.8
LNU249
26153.1
0.010
0.010
114.5


LNU288
14563.9
0.252
0.001
108.8
LNU249
26154.2
0.008
0.229
65.7


LNU288
14562.9
0.180
0.001
48.7
LNU249
26152.4
0.005
0.454
11.5


LNU288
14563.6
0.156
0.052
29.3
LNU249
26152.2
0.005
0.664
6.7


LNU288
14562.7
0.127
0.720
5.0
LNU282
27563.1
0.008
0.006
78.0


LNU75
27572.3
0.278
0.010
130.0
LNU282
27565.2
0.007
0.125
53.9


LNU75
27572.2
0.250
0.054
107.0
LNU282
27565.1
0.005
0.749
5.6


LNU75
27571.4
0.245
0.014
102.6
LNU288
14563.9
0.011
0.005
144.5


LNU75
27571.2
0.227
0.000
88.0
LNU288
14564.8
0.010
0.009
120.4


CONT.
—
0.144
—
0.0
LNU288
14562.9
0.007
0.048
58.7


LNU11
28204.3
0.154
0.501
7.1
LNU288
14563.6
0.007
0.085
52.8


LNU183
24863.1
0.152
0.610
5.5
LNU288
14562.7
0.006
0.116
24.9


LNU201
28222.2
0.161
0.452
11.8
LNU75
27572.2
0.014
0.054
192.2


LNU268
26045.1
0.163
0.186
13.5
LNU75
27572.3
0.012
0.011
153.1


CONT.
—
0.137
—
0.0
LNU75
27571.2
0.010
0.002
117.2


LNU11
28205.1
0.201
0.011
46.8
LNU75
27571.4
0.010
0.003
109.7


LNU11
28203.2
0.180
0.062
31.3
CONT.
—
0.006
—
0.0


LNU11
28204.1
0.150
0.569
9.5
LNU11
28204.3
0.007
0.121
26.6


LNU11
28202.5
0.150
0.669
9.5
LNU11
28205.2
0.006
0.737
7.7


LNU11
28205.2
0.143
0.634
4.5
LNU14
27823.2
0.006
0.686
2.1


LNU112
28212.1
0.178
0.123
29.8
LNU183
24863.1
0.006
0.752
4.3


LNU112
28212.4
0.156
0.058
14.2
LNU201
28222.2
0.006
0.679
9.4


LNU112
28212.3
0.155
0.158
12.9
LNU201
28223.1
0.006
0.773
3.0


LNU14
27821.4
0.179
0.108
30.8
LNU268
26044.2
0.007
0.291
21.0


LNU14
27821.3
0.177
0.103
29.4
LNU268
26045.1
0.007
0.087
18.0


LNU14
27824.2
0.162
0.113
18.6
CONT.
—
0.006
—
0.0


LNU183
24864.6
0.205
0.005
49.5
LNU11
28205.1
0.008
0.211
27.7


LNU183
24863.1
0.203
0.097
48.0
LNU11
28204.1
0.007
0.466
13.3


LNU183
24863.12
0.195
0.001
42.5
LNU11
28203.2
0.007
0.514
9.6


LNU183
24865.1
0.182
0.012
33.1
LNU11
28202.5
0.007
0.747
8.8


LNU191
28325.4
0.182
0.127
32.8
LNU112
28212.1
0.009
0.100
38.2


LNU191
28323.1
0.177
0.021
29.0
LNU112
28212.3
0.007
0.185
16.5


LNU191
28324.2
0.167
0.070
21.7
LNU112
28212.4
0.007
0.598
6.0


LNU191
28321.3
0.157
0.137
14.5
LNU14
27821.4
0.008
0.083
28.9


LNU201
28222.2
0.230
0.047
68.1
LNU14
27821.3
0.008
0.212
22.1


LNU201
28221.3
0.163
0.247
19.2
LNU14
27824.2
0.007
0.268
13.3


LNU201
28223.3
0.162
0.280
18.1
LNU183
24863.12
0.009
0.005
51.4


LNU268
26041.4
0.190
0.160
39.0
LNU183
24864.6
0.009
0.030
49.8


LNU268
26043.4
0.156
0.297
13.7
LNU183
24863.1
0.009
0.013
42.6


CONT.
—
0.126
—
0.0
LNU183
24865.1
0.009
0.029
42.6


LNU107
14584.9
0.221
0.167
74.4
LNU191
28325.4
0.009
0.063
37.8


LNU107
14583.8
0.175
0.117
38.3
LNU191
28323.1
0.008
0.017
30.5


LNU107
14585.5
0.137
0.598
8.5
LNU191
28324.2
0.007
0.554
7.2


LNU116
14492.5
0.201
0.237
58.9
LNU191
28321.3
0.007
0.689
5.2


LNU116
14494.5
0.188
0.192
48.8
LNU201
28222.2
0.012
0.014
86.3


LNU116
14492.9
0.178
0.105
40.7
LNU201
28221.3
0.009
0.158
38.6


LNU116
14493.6
0.160
0.439
26.3
LNU201
28223.3
0.007
0.290
20.1


LNU121
25642.2
0.233
0.043
83.8
LNU268
26041.4
0.009
0.138
44.2


LNU121
27713.4
0.190
0.006
50.0
LNU268
26043.4
0.007
0.746
5.2


LNU121
27711.1
0.188
0.010
48.7
CONT.
—
0.005
—
0.0


LNU121
27713.1
0.164
0.155
29.3
LNU107
14583.8
0.009
0.034
86.2


LNU126
25343.1
0.199
0.011
57.4
LNU107
14584.9
0.008
0.021
73.0


LNU126
25345.1
0.175
0.153
38.4
LNU107
14585.5
0.006
0.282
24.3


LNU126
25343.3
0.172
0.095
36.3
LNU116
14492.9
0.008
0.017
66.1


LNU158
27433.3
0.201
0.015
58.6
LNU116
14492.5
0.007
0.048
54.5


LNU158
27433.2
0.187
0.075
47.6
LNU116
14494.5
0.007
0.101
43.9


LNU158
27432.5
0.156
0.399
23.5
LNU116
14493.6
0.005
0.544
7.9


LNU177
24762.6
0.214
0.018
69.3
LNU121
25642.2
0.010
0.017
106.3


LNU182
25384.1
0.219
0.001
73.0
LNU121
27713.4
0.009
0.001
83.1


LNU182
25384.2
0.170
0.154
34.3
LNU121
27713.1
0.007
0.010
48.1


LNU182
25384.5
0.152
0.450
20.3
LNU121
27711.1
0.007
0.010
47.6


LNU182
27521.4
0.139
0.537
10.2
LNU126
25343.1
0.009
0.022
97.4


LNU2
25713.1
0.186
0.107
47.2
LNU126
25345.1
0.008
0.145
59.3


LNU2
27842.3
0.157
0.298
23.9
LNU126
25343.3
0.007
0.085
39.7


LNU2
27842.1
0.134
0.697
6.0
LNU158
27433.3
0.010
0.015
107.4


LNU225
25991.5
0.212
0.003
67.5
LNU158
27432.5
0.008
0.100
69.3


LNU225
25991.2
0.190
0.157
49.9
LNU158
27433.2
0.008
0.092
59.8


LNU239
26284.1
0.156
0.273
23.1
LNU177
24762.6
0.008
0.001
77.8


LNU239
26283.2
0.138
0.528
9.0
LNU177
24764.9
0.006
0.184
32.3


LNU83
27681.4
0.161
0.237
27.5
LNU177
24765.2
0.006
0.282
21.2


LNU83
27684.1
0.157
0.208
24.0
LNU182
25384.1
0.010
0.000
107.4


LNU83
27685.1
0.138
0.522
9.1
LNU182
25384.2
0.008
0.006
77.2


CONT.
—
0.118
—
0.0
LNU182
27521.4
0.007
0.006
39.2


LNU107
14584.9
0.230
0.000
95.3
LNU182
25384.5
0.006
0.172
30.7


LNU107
14585.2
0.178
0.001
50.9
LNU2
25713.1
0.008
0.060
74.1


LNU107
14583.8
0.161
0.043
36.1
LNU2
27842.3
0.007
0.037
43.9


LNU107
14585.5
0.128
0.604
8.2
LNU2
27842.1
0.006
0.035
27.5


LNU116
14492.5
0.235
0.000
98.7
LNU225
25991.5
0.011
0.000
136.0


LNU116
14493.6
0.211
0.001
78.9
LNU225
25991.2
0.010
0.186
115.3


LNU116
14494.5
0.186
0.118
57.7
LNU239
26284.1
0.007
0.012
40.7


LNU116
14492.9
0.157
0.046
33.4
LNU239
26283.2
0.005
0.576
9.5


LNU116
14491.5
0.146
0.044
23.7
LNU239
26281.1
0.005
0.686
9.0


LNU121
27711.1
0.220
0.007
86.6
LNU57
27854.5
0.005
0.373
13.2


LNU121
27713.4
0.218
0.004
84.4
LNU83
27684.1
0.007
0.162
47.1


LNU121
25642.2
0.215
0.002
81.9
LNU83
27681.4
0.007
0.169
41.1


LNU121
27713.1
0.209
0.000
76.9
CONT.
—
0.004
—
0.0


LNU121
27713.3
0.136
0.357
15.2
LNU107
14584.9
0.010
0.002
148.1


LNU126
25343.1
0.181
0.029
53.3
LNU107
14585.2
0.007
0.021
84.0


LNU126
25341.1
0.153
0.415
29.7
LNU107
14583.8
0.007
0.055
70.4


LNU126
25343.3
0.133
0.604
12.4
LNU107
14585.5
0.005
0.107
24.7


LNU126
25345.1
0.125
0.730
6.0
LNU116
14492.5
0.010
0.000
140.7


LNU158
27433.3
0.260
0.000
120.3
LNU116
14494.5
0.008
0.000
109.3


LNU158
27432.5
0.219
0.011
85.5
LNU116
14493.6
0.008
0.000
104.9


LNU158
27433.2
0.200
0.106
69.9
LNU116
14492.9
0.007
0.131
74.7


LNU158
27434.1
0.140
0.454
18.3
LNU116
14491.5
0.006
0.000
58.6


LNU177
24764.12
0.227
0.027
92.6
LNU121
25642.2
0.009
0.022
122.8


LNU177
24763.6
0.161
0.172
36.3
LNU121
27713.1
0.008
0.022
105.6


LNU177
24764.9
0.154
0.225
30.4
LNU121
27711.1
0.008
0.051
104.9


LNU182
25384.1
0.175
0.039
48.6
LNU121
27713.4
0.008
0.006
95.1


LNU182
25384.6
0.173
0.038
47.0
LNU121
27713.3
0.006
0.228
47.5


LNU182
25384.5
0.135
0.513
14.4
LNU126
25343.1
0.007
0.010
77.2


LNU2
27842.1
0.210
0.001
78.2
LNU126
25343.3
0.005
0.246
27.2


LNU2
27842.3
0.175
0.043
48.6
LNU126
25341.1
0.005
0.314
25.3


LNU2
25713.1
0.132
0.702
11.5
LNU126
25343.4
0.005
0.257
17.9


LNU2
27845.2
0.127
0.715
7.9
LNU126
25345.1
0.004
0.624
4.9


LNU225
25991.3
0.282
0.013
138.6
LNU158
27433.3
0.011
0.001
180.9


LNU225
25991.2
0.223
0.003
89.0
LNU158
27433.2
0.010
0.082
138.3


LNU225
25991.8
0.189
0.000
60.3
LNU158
27432.5
0.010
0.012
137.0


LNU225
25991.1
0.159
0.143
34.7
LNU177
24764.12
0.010
0.012
147.5


LNU239
26281.1
0.152
0.239
28.9
LNU177
24763.6
0.007
0.049
67.3


LNU239
26284.2
0.149
0.094
26.5
LNU177
24762.6
0.005
0.249
17.3


LNU239
26284.1
0.136
0.439
14.9
LNU177
24765.2
0.005
0.333
16.0


LNU239
26283.2
0.130
0.353
9.8
LNU182
25384.1
0.008
0.006
93.8


LNU57
27852.1
0.198
0.010
68.0
LNU182
25384.6
0.007
0.019
74.1


LNU57
27851.2
0.192
0.061
62.3
LNU182
25384.5
0.005
0.160
26.5


LNU57
27854.5
0.143
0.077
21.2
LNU182
25384.2
0.005
0.450
16.0


LNU57
27854.3
0.140
0.256
18.5
LNU2
27842.1
0.009
0.027
131.5


LNU83
27685.1
0.288
0.001
143.9
LNU2
27842.3
0.007
0.104
66.7


LNU83
27685.2
0.201
0.003
70.6
LNU2
25713.1
0.007
0.362
63.0


LNU83
27681.4
0.164
0.041
38.7
LNU2
27845.2
0.006
0.161
52.5


CONT.
—
0.127
—
0.0
LNU2
27845.3
0.005
0.028
30.2


LNU17
13991.1
0.145
0.39
14.5
LNU225
25991.2
0.011
0.007
161.1


LNU17
13993.9
0.135
0.71
6.1
LNU225
25991.3
0.010
0.051
139.5







LNU225
25991.8
0.007
0.001
64.2







LNU225
25991.1
0.005
0.067
31.5







LNU239
26284.2
0.007
0.054
63.6







LNU239
26281.1
0.006
0.190
54.9







LNU239
26283.2
0.006
0.043
41.4







LNU239
26284.1
0.005
0.414
14.8







LNU57
27852.1
0.009
0.000
133.3







LNU57
27851.2
0.009
0.006
110.5







LNU57
27854.5
0.006
0.046
41.4







LNU57
27854.3
0.005
0.404
19.8







LNU83
27685.1
0.010
0.000
156.2







LNU83
27685.2
0.009
0.000
111.7







LNU83
27681.4
0.007
0.018
80.9







LNU83
27682.1
0.004
0.479
6.8





Table 60.


“CONT.”—Control;


“Ave.”—Average;


“% Incr.” = % increment.













TABLE 61







Genes showing improved plant performance at


nitrogen deficient conditions (T2 generation)









Leaf Area [cm2]

















%



Gene Name
Event #
Average
p-value
increment

















CONTROL
—
0.559
—
0.0



LNU100
14474.3
0.877
0.032
56.8



LNU100
14473.1
0.646
0.097
15.5



LNU104
25033.3
0.794
0.005
41.9



LNU104
25034.1
0.742
0.047
32.6



LNU104
25032.2
0.581
0.749
3.8



LNU213
24654.4
1.040
0.036
86.0



LNU213
24653.2
0.896
0.000
60.1



LNU213
24651.1
0.607
0.343
8.5



LNU218
24781.7
0.777
0.116
38.8



LNU218
24783.2
0.775
0.086
38.5



LNU4
25134.2
0.858
0.002
53.4



LNU4
25134.1
0.703
0.183
25.6



LNU48
24802.2
0.897
0.009
60.3



LNU48
24803.2
0.757
0.005
35.3



LNU48
24804.4
0.726
0.066
29.8



LNU8
25063.1
0.974
0.002
74.0



LNU8
25063.6
0.863
0.111
54.3



LNU8
25062.2
0.593
0.706
5.9



LNU94
24833.3
0.761
0.034
36.0



LNU94
24834.4
0.692
0.208
23.7



LNU94
24834.1
0.680
0.234
21.6



CONTROL
—
0.765
—
0.0



LNU1
24681.3
0.979
0.168
28.0



LNU1
24684.1
0.918
0.237
20.1



LNU1
24682.1
0.916
0.285
19.8



LNU1
24682.2
0.879
0.477
14.9



LNU1
24681.1
0.848
0.515
10.9



LNU1
24683.2
0.822
0.641
7.5



LNU133
24744.3
1.103
0.062
44.3



LNU133
24741.1
1.024
0.081
33.8



LNU133
24744.2
0.822
0.651
7.5



LNU175
24732.4
1.207
0.033
57.8



LNU175
24732.1
1.192
0.019
55.9



LNU175
24734.4
0.938
0.207
22.7



LNU178
14614.5
1.132
0.047
48.0



LNU178
14611.5
0.986
0.123
28.9



LNU178
14612.1
0.832
0.567
8.8



LNU178
14611.1
0.809
0.729
5.8



LNU215
24664.3
1.083
0.061
41.6



LNU215
24661.4
0.820
0.659
7.2



LNU24
24973.1
1.022
0.103
33.7



LNU24
24971.2
0.985
0.119
28.8



LNU24
24971.4
0.971
0.301
27.0



LNU6
24992.3
1.049
0.063
37.2



LNU6
24994.1
0.885
0.347
15.8



LNU6
24993.3
0.855
0.506
11.8



LNU6
24994.2
0.815
0.696
6.6



LNU82
24823.1
0.911
0.252
19.1



LNU9
25001.3
0.892
0.361
16.7



LNU9
25003.1
0.879
0.416
14.9



LNU9
25001.1
0.829
0.663
8.4



CONTROL
—
0.561
—
0.0



LNU120
25463.7
0.758
0.001
35.0



LNU120
25463.3
0.749
0.003
33.4



LNU124
14501.7
0.767
0.019
36.6



LNU124
14501.1
0.666
0.072
18.6



LNU124
14502.7
0.645
0.018
14.9



LNU132
14102.7
0.622
0.288
10.9



LNU132
14102.9
0.622
0.258
10.8



LNU140
14112.7
0.953
0.000
69.8



LNU140
14111.6
0.686
0.046
22.2



LNU140
14112.6
0.593
0.465
5.6



LNU140
14114.8
0.582
0.553
3.7



LNU180
24724.3
0.975
0.003
73.6



LNU180
24723.3
0.774
0.044
37.8



LNU196
25534.1
0.674
0.165
20.0



LNU196
25533.1
0.631
0.476
12.4



LNU20
24932.4
0.644
0.190
14.6



LNU20
24933.2
0.640
0.421
14.0



LNU36
25562.3
0.860
0.107
53.1



LNU36
25562.4
0.574
0.750
2.2



LNU71
25853.4
0.835
0.005
48.8



CONTROL
—
0.597
—
0.0



LNU1
24681.3
0.695
0.438
16.4



LNU110
24952.3
0.872
0.056
46.1



LNU110
24953.3
0.789
0.110
32.1



LNU110
24953.2
0.674
0.308
12.9



LNU175
24732.2
0.832
0.032
39.4



LNU175
24733.4
0.725
0.105
21.3



LNU19
25151.1
0.881
0.006
47.6



LNU19
25153.3
0.746
0.119
25.0



LNU215
24664.2
0.846
0.000
41.8



LNU215
24663.4
0.735
0.007
23.1



LNU215
24663.3
0.648
0.325
8.5



LNU27
24873.1
0.899
0.039
50.5



LNU27
24873.4
0.742
0.035
24.3



LNU27
24872.4
0.647
0.344
8.3



LNU44
24924.3
1.069
0.023
79.0



LNU44
24924.2
0.826
0.027
38.3



LNU44
24922.3
0.706
0.156
18.2



LNU54
24903.5
1.040
0.002
74.1



LNU54
24901.2
0.902
0.042
51.1



LNU54
24903.3
0.743
0.098
24.5



LNU79
24884.4
0.963
0.010
61.3



LNU79
24881.1
0.850
0.006
42.4



LNU79
24884.3
0.742
0.061
24.3



LNU79
24882.2
0.666
0.164
11.5



CONTROL
—
0.764
—
0.0



LNU109
24891.5
1.021
0.020
33.7



LNU109
24892.5
0.939
0.200
22.9



LNU109
24891.2
0.908
0.308
18.8



LNU109
24892.6
0.882
0.367
15.5



LNU110
24952.1
1.492
0.000
95.2



LNU110
24953.2
1.101
0.014
44.1



LNU110
24952.3
1.054
0.094
37.9



LNU133
24744.3
1.192
0.002
56.1



LNU133
24741.1
1.023
0.009
33.9



LNU133
24741.2
0.961
0.005
25.8



LNU133
24744.2
0.795
0.706
4.0



LNU19
25151.1
1.069
0.003
39.9



LNU27
24873.4
1.224
0.000
60.2



LNU44
24922.3
1.153
0.000
50.9



LNU44
24924.3
0.915
0.236
19.8



LNU44
24923.1
0.820
0.501
7.3



LNU54
24901.2
1.131
0.002
48.1



LNU54
24903.5
1.035
0.037
35.4



LNU54
24902.4
0.938
0.009
22.7



LNU6
24994.5
1.104
0.015
44.5



LNU6
24992.3
1.026
0.021
34.2



LNU79
24884.4
1.154
0.001
51.0



LNU79
24881.1
1.031
0.029
34.9



LNU79
24882.2
0.958
0.080
25.3



LNU79
24884.3
0.855
0.382
12.0



CONTROL
—
0.512
—
0.0



LNU109
24891.2
1.067
0.004
108.5



LNU109
24892.8
1.053
0.000
105.8



LNU109
24891.5
0.737
0.082
43.9



LNU109
24892.5
0.600
0.244
17.2



LNU143
25975.3
0.755
0.030
47.5



LNU143
25972.1
0.734
0.029
43.3



LNU143
25975.2
0.694
0.055
35.6



LNU143
25971.5
0.623
0.129
21.7



LNU143
25971.2
0.560
0.491
9.5



LNU154
14604.7
0.936
0.002
82.9



LNU154
14604.6
0.843
0.015
64.7



LNU154
14601.6
0.804
0.070
57.1



LNU154
14602.8
0.692
0.156
35.2



LNU154
14604.4
0.672
0.077
31.2



LNU196
25532.2
1.156
0.001
125.8



LNU196
25534.1
0.890
0.025
73.9



LNU196
25531.2
0.727
0.028
42.0



LNU196
25532.1
0.626
0.373
22.2



LNU207
24642.5
0.947
0.003
85.0



LNU207
24642.4
0.914
0.004
78.5



LNU207
24644.18
0.804
0.009
57.1



LNU207
24644.13
0.561
0.593
9.5



LNU207
24641.1
0.532
0.760
4.0



LNU288
14562.12
0.735
0.024
43.5



LNU288
14562.7
0.708
0.037
38.2



LNU288
14562.1
0.658
0.173
28.5



LNU288
14564.9
0.637
0.101
24.5



LNU288
14562.9
0.633
0.180
23.6



LNU50
26024.2
0.863
0.013
68.6



LNU50
26023.2
0.740
0.016
44.6



LNU50
26025.4
0.644
0.088
25.8



LNU50
26022.1
0.577
0.407
12.7



LNU50
26023.5
0.559
0.452
9.2



LNU52
25723.2
1.263
0.000
146.7



LNU52
25721.4
0.968
0.048
89.0



LNU52
25721.3
0.888
0.006
73.4



LNU52
25723.1
0.534
0.750
4.4



CONTROL
—
0.646
—
0.0



LNU143
25975.2
0.707
0.290
9.4



LNU154
14602.8
0.829
0.010
28.2



LNU154
14604.4
0.759
0.253
17.4



LNU154
14601.6
0.708
0.296
9.6



LNU207
24642.5
1.001
0.002
54.9



LNU207
24641.1
0.823
0.047
27.3



LNU207
24642.4
0.718
0.180
11.1



LNU211
24774.4
2.327
0.386
260.0



LNU52
25721.1
0.934
0.011
44.5



LNU52
25723.2
0.872
0.025
34.8



LNU52
25723.1
0.848
0.118
31.2



LNU52
25721.2
0.771
0.151
19.3



LNU69
14571.1
0.824
0.041
27.5



LNU69
14572.9
0.790
0.167
22.2



LNU69
14572.8
0.759
0.195
17.4



CONTROL
—
0.727
—
0.0



LNU150
24842.9
1.108
0.000
52.3



LNU150
24841.9
0.795
0.437
9.3



LNU179
24632.5
0.939
0.038
29.0



LNU179
24631.9
0.785
0.612
7.9



LNU232
26003.7
0.798
0.443
9.7



LNU235
26184.4
0.947
0.035
30.2



LNU235
26185.3
0.941
0.203
29.4



LNU242
25473.1
0.907
0.012
24.6



LNU242
25474.1
0.783
0.529
7.6



LNU242
25471.1
0.747
0.760
2.7



LNU76
26423.1
0.971
0.002
33.5



LNU76
26421.2
0.836
0.200
14.9



LNU76
26425.1
0.832
0.444
14.3



LNU95
13985.11
0.973
0.012
33.8



LNU95
13985.12
0.801
0.537
10.2



CONTROL
—
0.678
—
0.0



LNU118
14013.6
0.809
0.165
19.4



LNU118
14012.15
0.784
0.111
15.7



LNU150
24841.9
0.798
0.251
17.8



LNU150
24841.6
0.753
0.467
11.1



LNU150
24842.9
0.714
0.775
5.4



LNU179
24631.6
0.828
0.134
22.2



LNU179
24631.7
0.812
0.089
19.9



LNU179
24632.7
0.736
0.202
8.6



LNU179
24631.9
0.709
0.743
4.6



LNU232
26001.5
0.814
0.170
20.2



LNU232
26003.3
0.707
0.611
4.3



LNU235
26185.2
1.143
0.000
68.7



LNU235
26184.4
1.125
0.000
66.0



LNU235
26184.2
0.975
0.001
44.0



LNU242
25474.1
0.884
0.056
30.5



LNU288
14563.9
0.967
0.029
42.8



LNU288
14562.1
0.937
0.025
38.3



LNU288
14564.9
0.838
0.020
23.6



LNU288
14562.7
0.781
0.228
15.2



LNU76
26421.2
0.880
0.269
29.8



LNU76
26423.1
0.838
0.247
23.7



LNU76
26422.2
0.824
0.154
21.6



LNU95
13985.16
1.257
0.001
85.6



LNU95
13985.15
1.211
0.000
78.7



LNU95
13985.12
0.949
0.003
40.1



LNU95
13985.19
0.745
0.358
9.9



CONTROL
—
0.644
—
0.0



LNU101
27632.7
0.828
0.020
28.6



LNU101
27632.1
0.664
0.486
3.2



LNU128
26515.3
0.894
0.004
38.8



LNU192
28315.2
0.798
0.011
23.9



LNU192
28313.2
0.657
0.721
2.1



LNU206
27621.2
0.750
0.086
16.5



LNU211
24771.1
0.708
0.169
10.0



LNU282
27563.3
0.835
0.029
29.7



LNU282
27563.1
0.800
0.089
24.3



LNU282
27562.1
0.750
0.319
16.5



LNU69
14571.1
0.896
0.001
39.3



LNU69
14572.9
0.696
0.553
8.1



LNU75
27572.1
0.745
0.054
15.7



LNU75
27572.2
0.730
0.246
13.5



CONTROL
—
0.564
—
0.0



LNU206
27621.2
0.981
0.131
73.9



LNU206
27621.1
0.863
0.037
52.9



LNU206
27622.1
0.791
0.037
40.2



LNU206
27622.4
0.707
0.099
25.3



LNU249
26153.1
0.869
0.008
54.0



LNU249
26154.2
0.738
0.044
30.7



LNU249
26152.4
0.605
0.555
7.2



LNU282
27563.1
0.761
0.014
34.7



LNU282
27565.2
0.753
0.097
33.4



LNU288
14563.9
0.883
0.000
56.4



LNU288
14564.8
0.854
0.009
51.2



LNU288
14562.9
0.698
0.015
23.6



LNU288
14563.6
0.634
0.331
12.3



LNU75
27572.3
0.949
0.000
68.1



LNU75
27572.2
0.829
0.064
46.9



LNU75
27571.4
0.795
0.000
40.8



LNU75
27571.2
0.781
0.005
38.3



CONTROL
—
0.721
—
0.0



LNU11
28204.3
0.762
0.570
5.7



LNU112
28212.4
0.757
0.585
5.0



LNU14
27823.2
0.776
0.442
7.7



LNU183
24863.1
0.926
0.009
28.4



LNU183
24863.12
0.915
0.019
26.9



LNU183
24864.6
0.812
0.539
12.7



LNU183
24865.1
0.795
0.318
10.3



LNU201
28223.1
0.821
0.208
14.0



LNU201
28222.2
0.746
0.733
3.6



LNU268
26044.2
0.805
0.386
11.7



LNU268
26045.1
0.761
0.572
5.6



CONTROL
—
0.666
—
0.0



LNU11
28205.1
0.858
0.048
28.9



LNU11
28204.1
0.729
0.372
9.5



LNU11
28205.2
0.708
0.399
6.3



LNU112
28212.4
0.772
0.015
16.0



LNU112
28212.1
0.760
0.022
14.1



LNU112
28212.3
0.690
0.781
3.7



LNU14
27821.3
0.826
0.009
24.1



LNU14
27821.4
0.813
0.053
22.2



LNU14
27824.2
0.708
0.564
6.4



LNU183
24864.6
1.097
0.018
64.7



LNU183
24863.12
0.968
0.016
45.5



LNU183
24865.1
0.954
0.031
43.4



LNU183
24863.1
0.881
0.002
32.3



LNU191
28325.4
0.775
0.076
16.3



LNU191
28324.2
0.756
0.146
13.5



LNU191
28321.3
0.690
0.556
3.6



LNU201
28222.2
0.929
0.017
39.6



LNU201
28223.3
0.788
0.167
18.3



LNU268
26041.4
0.741
0.203
11.2



LNU268
26043.4
0.722
0.338
8.5



CONTROL
—
0.523
—
0.0



LNU107
14583.8
0.794
0.010
51.7



LNU107
14585.5
0.656
0.055
25.4



LNU107
14584.9
0.597
0.505
14.2



LNU116
14494.5
0.877
0.002
67.7



LNU116
14492.9
0.728
0.082
39.1



LNU116
14492.5
0.664
0.045
27.0



LNU116
14493.6
0.550
0.734
5.1



LNU121
25642.2
0.892
0.013
70.5



LNU121
27711.1
0.880
0.001
68.2



LNU121
27713.4
0.878
0.000
67.7



LNU121
27713.1
0.799
0.055
52.7



LNU126
25343.1
0.723
0.170
38.2



LNU126
25345.1
0.713
0.065
36.3



LNU126
25343.3
0.638
0.325
21.9



LNU158
27433.3
0.797
0.030
52.4



LNU158
27433.2
0.793
0.055
51.5



LNU158
27432.5
0.670
0.383
28.1



LNU177
24762.6
0.950
0.000
81.6



LNU177
24764.9
0.637
0.256
21.7



LNU177
24765.2
0.564
0.702
7.7



LNU182
25384.1
0.855
0.016
63.4



LNU182
25384.5
0.649
0.148
24.1



LNU182
25384.2
0.641
0.348
22.5



LNU182
27521.4
0.640
0.063
22.4



LNU2
27842.1
0.621
0.249
18.7



LNU2
25713.1
0.585
0.478
11.8



LNU2
27845.2
0.582
0.352
11.3



LNU2
27842.3
0.566
0.556
8.1



LNU225
25991.5
0.686
0.194
31.1



LNU225
25991.2
0.625
0.456
19.6



LNU239
26283.2
0.628
0.351
20.0



LNU239
26284.1
0.602
0.230
15.0



LNU57
27854.5
0.548
0.725
4.7



LNU83
27684.1
0.790
0.021
51.1



CONTROL
—
0.400
—
0.0



LNU107
14584.9
0.579
0.031
44.7



LNU107
14585.2
0.511
0.131
27.8



LNU107
14583.8
0.461
0.129
15.2



LNU107
14585.5
0.420
0.690
5.0



LNU116
14492.5
0.713
0.020
78.1



LNU116
14494.5
0.702
0.015
75.3



LNU116
14492.9
0.589
0.061
47.2



LNU116
14493.6
0.563
0.130
40.7



LNU116
14491.5
0.489
0.343
22.2



LNU121
27713.1
0.833
0.031
108.0



LNU121
27711.1
0.636
0.082
58.9



LNU121
27713.4
0.635
0.003
58.8



LNU121
25642.2
0.557
0.065
39.2



LNU126
25343.1
0.559
0.086
39.7



LNU126
25343.3
0.521
0.289
30.1



LNU126
25341.1
0.472
0.329
18.0



LNU158
27433.3
0.898
0.001
124.4



LNU158
27432.5
0.712
0.035
77.8



LNU158
27433.2
0.649
0.056
62.2



LNU158
27434.5
0.415
0.797
3.6



LNU177
24764.12
0.500
0.376
24.8



LNU177
24763.6
0.453
0.543
13.1



LNU177
24762.6
0.449
0.578
12.1



LNU182
25384.6
0.609
0.015
52.2



LNU182
25384.2
0.542
0.059
35.3



LNU2
27842.1
0.653
0.007
63.1



LNU2
27842.3
0.549
0.109
37.1



LNU2
27845.3
0.435
0.627
8.5



LNU225
25991.2
0.693
0.001
73.2



LNU225
25991.3
0.673
0.058
68.2



LNU225
25991.8
0.605
0.000
51.1



LNU225
25991.1
0.456
0.393
13.9



LNU239
26283.2
0.513
0.120
28.2



LNU239
26284.1
0.477
0.331
19.3



LNU239
26284.2
0.468
0.353
16.9



LNU239
26281.1
0.465
0.317
16.1



LNU57
27854.5
0.496
0.354
24.0



LNU57
27852.1
0.478
0.152
19.5



LNU57
27851.2
0.441
0.510
10.1



LNU57
27854.3
0.424
0.779
6.0



LNU83
27685.1
0.793
0.009
98.1



LNU83
27685.2
0.579
0.123
44.7



LNU83
27681.4
0.507
0.120
26.6



CONTROL
12033.3
0.426
—
0.0



LNU129
27501.2
0.681
<0.05
59.9



LNU129
27502.4
0.579
<0.05
36.0



LNU129
27503.4
0.475
<0.6
11.4



LNU129
27504.2
0.779
<0.05
82.9



LNU129
27504.3
0.657
<0.05
54.2



LNU147
27512.1
0.550
<0.05
29.1



LNU147
27513.2
0.554
<0.05
30.1



LNU147
27514.1
0.552
<0.05
29.7



LNU147
27514.2
0.730
<0.05
71.4



LNU153
24851.3
0.621
<0.05
45.9



LNU153
24851.4
0.504
<0.5
18.3



LNU189
26382.3
0.950
<0.05
122.9



LNU189
26382.4
0.779
<0.05
82.9



LNU189
26383.1
0.613
<0.05
43.9



LNU189
26385.1
0.688
<0.05
61.6



LNU189
26385.2
0.493
<0.6
15.8



LNU219
27461.1
0.804
<0.05
88.7



LNU219
27462.1
0.781
<0.05
83.3



LNU219
27462.2
0.903
<0.05
112.0



LNU219
27464.1
0.622
<0.05
46.0



LNU219
27464.2
0.458
<0.6
7.4



LNU256
26211.2
0.519
<0.2
21.8



LNU256
26212.3
0.864
<0.05
102.9



LNU256
26213.1
0.617
<0.05
44.9



LNU256
26214.1
0.667
<0.05
56.6



LNU257
26254.1
0.568
<0.05
33.4



LNU257
26254.3
0.980
<0.05
130.0



LNU257
26254.7
0.842
<0.05
97.6



LNU257
26255.2
0.515
<0.2
20.9



LNU257
26255.3
0.505
<0.05
18.4



LNU261
27401.4
0.527
<0.2
23.7



LNU261
27402.4
0.477
<0.05
11.9



LNU261
27403.2
0.728
<0.05
71.0



LNU261
27405.1
0.609
<0.05
42.9



LNU261
27405.3
0.537
<0.1
26.0



LNU33
25552.2
0.745
<0.05
74.9



LNU33
25553.2
0.571
<0.05
34.1



LNU33
25553.3
0.594
<0.05
39.5



LNU35
27421.2
0.713
<0.05
67.5



LNU35
27422.1
0.556
<0.05
30.4



LNU35
27423.3
0.859
<0.05
101.6



LNU35
27424.3
0.662
<0.05
55.4



LNU35
27424.4
0.593
<0.05
39.2



LNU50
26022.1
0.853
<0.05
100.1



LNU50
26023.2
0.839
<0.05
96.9



LNU50
26023.3
0.501
<0.05
17.6



LNU50
26024.1
0.557
<0.05
30.7



LNU50
26025.3
1.031
<0.05
142.1



LNU70
25311.4
0.835
<0.05
96.0



LNU70
25313.1
0.534
<0.05
25.3



LNU70
25313.2
0.801
<0.05
88.0



LNU70
25315.1
0.501
<0.2
17.7







Table 61.













TABLE 62







Genes showing improved plant performance at nitrogen deficient


conditions (T1 generation)











Plant Biomass Fresh

Plant Biomass Dry



Weight [gr.]

Weight [gr.]














Gene

p-

Gene

p-
%


Name
Ave.
value
% incr.
Name
Ave.
value
incr.

















CONTROL
0.145
—
0.0
CONTROL
0.008
—
0.0


LNU154
0.146
0.982
0.4
LNU121
0.008
0.715
6.8


CONTROL
0.099
—
0.0
CONTROL
0.005
—
0.0


LNU127
0.106
0.594
7.3
LNU127
0.006
0.595
8.1


LNU188
0.110
0.481
11.4
LNU188
0.005
0.890
2.9


LNU2
0.108
0.419
9.8
LNU239
0.006
0.552
18.1


LNU239
0.110
0.642
11.2
LNU255
0.007
0.245
27.1


LNU255
0.116
0.170
17.9
LNU258
0.006
0.806
6.7


LNU265
0.102
0.740
3.7
LNU265
0.006
0.408
13.8


LNU275
0.137
0.080
39.1
LNU275
0.007
0.079
35.7


LNU58
0.119
0.239
20.6
LNU32
0.008
0.018
45.7


LNU83
0.111
0.621
12.6
LNU58
0.008
0.033
51.9


CONTROL
0.126
—
0.0
CONTROL
0.005
—
0.0


LNU115
0.133
0.698
5.2
LNU176
0.006
0.136
14.0


CONTROL
0.116
—
0.0
LNU284
0.005
0.855
3.2


LNU225
0.127
0.548
9.9
CONTROL
0.006
—
0.0


LNU262
0.128
0.317
10.5
LNU115
0.006
0.775
5.8


LNU59
0.117
0.921
1.6
CONTROL
0.005
—
0.0


LNU60
0.148
0.188
27.9
LNU225
0.005
0.527
9.4






LNU262
0.006
0.081
32.4






LNU266
0.006
0.247
24.4






LNU29
0.005
0.614
10.5






LNU59
0.006
0.195
33.0






LNU60
0.006
0.208
38.9





Table 62.


“Ave.”—Average;


“% Incr.” = % increment.













TABLE 63







Genes showing improved plant performance at nitrogen


deficient conditions (T1 generation)









Leaf Area [cm2]











Gene Name
Time Point
Average
p-value
% increment














CONTROL
Leaf_Area_TP2
0.283
—
0.0


LNU154
Leaf_Area_TP2
0.290
0.703
2.4


CONTROL
Leaf_Area_TP3
0.571
—
0.0


LNU154
Leaf_Area_TP3
0.582
0.817
1.9


CONTROL
Leaf_Area_TP1
0.104
—
0.0


LNU127
Leaf_Area_TP1
0.113
0.515
8.3


LNU275
Leaf_Area_TP1
0.156
0.001
49.6


CONTROL
Leaf_Area_TP2
0.269
—
0.0


LNU127
Leaf_Area_TP2
0.287
0.447
6.6


LNU2
Leaf_Area_TP2
0.302
0.123
12.4


LNU255
Leaf_Area_TP2
0.272
0.896
1.2


LNU275
Leaf_Area_TP2
0.404
0.000
50.1


LNU83
Leaf_Area_TP2
0.291
0.500
8.1


CONTROL
Leaf_Area_TP3
0.639
—
0.0


LNU127
Leaf_Area_TP3
0.647
0.862
1.3


LNU188
Leaf_Area_TP3
0.655
0.867
2.6


LNU2
Leaf_Area_TP3
0.662
0.602
3.6


LNU58
Leaf_Area_TP3
0.714
0.443
11.9


LNU83
Leaf_Area_TP3
0.677
0.705
6.1


CONTROL
Leaf_Area_TP1
0.118
—
0.0


LNU123
Leaf_Area_TP1
0.120
0.881
1.5


LNU134
Leaf_Area_TP1
0.138
0.038
17.3


LNU198
Leaf_Area_TP1
0.119
0.901
1.0


CONTROL
Leaf_Area_TP2
0.329
—
0.0


LNU134
Leaf_Area_TP2
0.346
0.587
5.1


CONTROL
Leaf_Area_TP1
0.097
—
0.0


LNU262
Leaf_Area_TP1
0.120
0.161
23.9


LNU266
Leaf_Area_TP1
0.107
0.475
10.8


LNU59
Leaf_Area_TP1
0.099
0.746
2.7


LNU60
Leaf_Area_TP1
0.102
0.491
5.8


CONTROL
Leaf_Area_TP2
0.271
—
0.0


LNU262
Leaf_Area_TP2
0.299
0.009
10.4


LNU60
Leaf_Area_TP2
0.278
0.808
2.6


CONTROL
Leaf_Area_TP3
0.567
—
0.0


LNU243
Leaf_Area_TP3
0.573
0.910
1.0


LNU262
Leaf_Area_TP3
0.618
0.253
9.0


LNU60
Leaf_Area_TP3
0.600
0.649
5.7





Table 63.






The genes presented in Tables 64-65 showed a significant improvement in plant NUE since they produced a larger root biomass (root length and root coverage) when grown under limiting nitrogen growth conditions, compared to control plants. Plants producing larger root biomass have better possibilities to absorb larger amount of nitrogen from soil. The genes were cloned under the regulation of a constitutive promoter (At6669) or root preferred promoter (RootP). The evaluation of each gene was performed by testing the performance of different number of events. Some of the genes were evaluated in more than one tissue culture assay. This second experiment confirmed the significant increment in root performance. Event with p-value <0.1 was considered statistically significant







TABLE 64







Genes showing improved root performance at nitrogen deficient conditions (T2 generation)











Roots Length [cm]

Roots Coverage [cm2]
















Gene


p-

Gene


p-
%


Name
Event #
Ave.
value
% incr.
Name
Event #
Ave.
value
incr.



















CONTROL
—
5.460
—
0.0
CONT.
—
6.069
—
0.0


LNU100
14474.3
7.008
0.000
28.3
LNU100
14474.3
9.816
0.031
61.7


LNU100
14471.4
6.667
0.013
22.1
LNU100
14473.1
6.948
0.313
14.5


LNU100
14473.1
6.461
0.026
18.3
LNU100
14471.4
6.929
0.190
14.2


LNU100
14474.4
6.309
0.001
15.5
LNU104
25033.3
7.325
0.131
20.7


LNU104
25034.1
6.942
0.283
27.1
LNU104
25034.1
6.679
0.275
10.0


LNU104
25033.3
6.252
0.044
14.5
LNU213
24653.2
9.230
0.002
52.1


LNU104
25033.1
5.902
0.151
8.1
LNU213
24654.4
8.585
0.198
41.4


LNU104
25032.2
5.707
0.571
4.5
LNU213
24653.1
6.920
0.321
14.0


LNU104
25032.1
5.563
0.688
1.9
LNU213
24651.1
6.367
0.523
4.9


LNU213
24653.2
6.677
0.003
22.3
LNU218
24783.2
8.791
0.090
44.8


LNU213
24653.1
6.371
0.056
16.7
LNU218
24781.7
6.766
0.632
11.5


LNU213
24654.4
6.357
0.060
16.4
LNU4
25134.2
7.955
0.057
31.1


LNU213
24651.1
6.202
0.002
13.6
LNU4
25134.1
6.933
0.347
14.2


LNU213
24652.4
6.124
0.053
12.2
LNU4
25131.1
6.521
0.371
7.4


LNU218
24783.2
6.533
0.071
19.6
LNU48
24802.2
10.147
0.001
67.2


LNU218
24781.1
6.273
0.001
14.9
LNU48
24804.4
8.757
0.018
44.3


LNU218
24781.7
5.757
0.545
5.4
LNU48
24802.1
6.538
0.497
7.7


LNU218
24781.2
5.575
0.526
2.1
LNU48
24803.2
6.474
0.601
6.7


LNU4
25131.1
6.811
0.000
24.7
LNU8
25063.1
11.923
0.014
96.4


LNU4
25134.2
6.809
0.000
24.7
LNU8
25063.6
7.772
0.469
28.0


LNU4
25134.1
6.092
0.453
11.6
LNU8
25062.2
6.228
0.714
2.6


LNU4
25133.3
5.643
0.584
3.3
LNU94
24833.3
7.631
0.136
25.7


LNU48
24804.4
6.762
0.001
23.9
LNU94
24834.4
6.753
0.519
11.3


LNU48
24802.2
6.606
0.003
21.0
CONT.
—
7.564
—
0.0


LNU48
24803.2
5.911
0.242
8.3
LNU1
24684.1
10.889
0.001
44.0


LNU48
24802.1
5.777
0.362
5.8
LNU1
24682.2
10.883
0.255
43.9


LNU8
25063.1
7.395
0.008
35.4
LNU1
24681.1
10.585
0.067
39.9


LNU8
25062.2
6.064
0.213
11.1
LNU1
24681.3
9.005
0.160
19.0


LNU8
25062.1
5.830
0.105
6.8
LNU1
24682.1
8.490
0.214
12.2


LNU8
25063.6
5.778
0.674
5.8
LNU133
24744.3
13.831
0.022
82.8


LNU94
24833.3
6.716
0.002
23.0
LNU133
24741.1
12.418
0.009
64.2


LNU94
24834.4
6.389
0.022
17.0
LNU133
24741.2
9.300
0.015
22.9


LNU94
24833.1
5.531
0.737
1.3
LNU133
24744.2
8.947
0.166
18.3


CONT.
—
6.635
—
0.0
LNU133
24742.2
8.706
0.318
15.1


LNU1
24684.1
7.260
0.000
9.4
LNU175
24732.4
16.185
0.044
114.0


LNU1
24681.1
7.070
0.179
6.6
LNU175
24732.1
15.203
0.003
101.0


LNU133
24744.3
7.399
0.028
11.5
LNU175
24734.4
10.409
0.050
37.6


LNU133
24741.2
7.040
0.003
6.1
LNU175
24731.2
8.880
0.165
17.4


LNU133
24741.1
6.975
0.033
5.1
LNU175
24733.4
8.589
0.374
13.6


LNU133
24742.2
6.937
0.281
4.6
LNU178
14611.5
13.504
0.018
78.5


LNU175
24732.1
7.839
0.003
18.2
LNU178
14611.1
10.597
0.123
40.1


LNU175
24732.4
7.594
0.135
14.5
LNU178
14614.5
10.444
0.173
38.1


LNU175
24734.4
7.437
0.021
12.1
LNU178
14611.4
8.568
0.168
13.3


LNU175
24733.4
6.965
0.158
5.0
LNU178
14612.1
8.562
0.317
13.2


LNU178
14611.5
7.651
0.025
15.3
LNU215
24664.3
12.121
0.051
60.2


LNU178
14614.5
7.431
0.081
12.0
LNU215
24661.4
9.556
0.117
26.3


LNU178
14611.1
7.198
0.027
8.5
LNU215
24664.2
9.327
0.011
23.3


LNU178
14612.1
6.985
0.005
5.3
LNU215
24663.4
8.874
0.121
17.3


LNU178
14611.4
6.889
0.092
3.8
LNU24
24973.1
12.870
0.010
70.1


LNU215
24664.3
7.586
0.001
14.3
LNU24
24971.4
11.398
0.014
50.7


LNU215
24661.4
7.127
0.146
7.4
LNU24
24971.2
10.449
0.001
38.1


LNU215
24664.2
7.087
0.002
6.8
LNU24
24972.1
8.338
0.479
10.2


LNU215
24663.4
7.006
0.167
5.6
LNU24
24971.3
8.036
0.493
6.2


LNU24
24973.1
7.751
0.000
16.8
LNU6
24992.3
12.812
0.028
69.4


LNU24
24971.4
7.021
0.243
5.8
LNU6
24994.2
10.067
0.055
33.1


LNU6
24992.3
7.266
0.108
9.5
LNU6
24993.3
9.314
0.126
23.1


LNU6
24994.2
7.159
0.021
7.9
LNU6
24994.5
9.037
0.390
19.5


LNU6
24994.5
7.027
0.388
5.9
LNU6
24994.1
8.410
0.406
11.2


LNU82
24823.1
7.050
0.186
6.3
LNU82
24823.1
10.020
0.110
32.5


LNU82
24824.2
7.029
0.092
5.9
LNU82
24824.2
9.584
0.087
26.7


LNU82
24824.3
6.741
0.511
1.6
LNU82
24824.3
8.475
0.310
12.0


LNU9
25001.1
7.345
0.072
10.7
LNU82
24824.1
8.462
0.562
11.9


LNU9
25003.1
7.308
0.021
10.2
LNU9
25001.1
10.896
0.012
44.0


LNU9
25001.3
7.027
0.076
5.9
LNU9
25003.1
10.296
0.099
36.1


LNU9
25001.2
6.762
0.730
1.9
LNU9
25001.2
9.602
0.217
26.9


CONT.
—
6.780
—
0.0
LNU9
25001.3
9.441
0.085
24.8


LNU120
25463.7
7.505
0.017
10.7
CONT.
—
7.438
—
0.0


LNU120
25463.3
7.104
0.329
4.8
LNU120
25463.7
11.666
0.000
56.8


LNU124
14502.1
7.180
0.348
5.9
LNU120
25463.3
8.314
0.249
11.8


LNU132
14102.7
7.563
0.022
11.6
LNU124
14502.1
9.538
0.258
28.2


LNU132
14102.9
7.351
0.021
8.4
LNU124
14501.7
9.283
0.341
24.8


LNU140
14112.6
7.272
0.087
7.3
LNU124
14502.7
8.218
0.448
10.5


LNU140
14112.7
7.233
0.076
6.7
LNU124
14501.1
7.888
0.721
6.0


LNU140
14114.8
7.140
0.189
5.3
LNU132
14102.7
9.324
0.165
25.4


LNU140
14111.6
6.988
0.530
3.1
LNU132
14102.9
9.217
0.025
23.9


LNU180
24724.3
7.931
0.000
17.0
LNU140
14112.7
12.797
0.001
72.0


LNU180
24723.3
6.980
0.532
3.0
LNU140
14111.6
9.362
0.025
25.9


LNU20
24932.4
6.886
0.733
1.6
LNU140
14112.6
7.974
0.374
7.2


LNU36
25562.3
7.270
0.279
7.2
LNU140
14114.8
7.954
0.540
6.9


LNU36
25562.4
7.219
0.142
6.5
LNU180
24724.3
13.032
0.003
75.2


CONT.
—
6.252
—
0.0
LNU180
24723.3
10.374
0.006
39.5


LNU1
24682.1
7.163
0.035
14.6
LNU180
24721.4
7.814
0.644
5.1


LNU1
24681.1
7.118
0.033
13.8
LNU20
24932.4
9.646
0.029
29.7


LNU1
24684.1
6.592
0.311
5.4
LNU20
24933.2
8.359
0.594
12.4


LNU110
24953.2
7.215
0.025
15.4
LNU36
25562.3
11.458
0.143
54.0


LNU110
24954.3
6.459
0.513
3.3
LNU36
25562.4
8.949
0.356
20.3


LNU110
24952.3
6.390
0.745
2.2
LNU71
25853.4
9.497
0.169
27.7


LNU175
24734.4
6.980
0.074
11.6
CONT.
—
7.471
—
0.0


LNU175
24732.2
6.789
0.217
8.6
LNU1
24682.1
7.871
0.605
5.3


LNU175
24732.1
6.684
0.291
6.9
LNU110
24952.3
9.457
0.430
26.6


LNU175
24733.4
6.405
0.644
2.4
LNU110
24953.2
7.893
0.671
5.6


LNU215
24664.2
7.333
0.014
17.3
LNU175
24732.2
10.614
0.087
42.1


LNU215
24663.4
7.264
0.028
16.2
LNU175
24732.1
8.454
0.362
13.1


LNU215
24661.4
7.235
0.020
15.7
LNU19
25151.1
8.732
0.331
16.9


LNU215
24663.3
7.010
0.122
12.1
LNU215
24664.2
10.890
0.014
45.8


LNU215
24663.1
6.620
0.320
5.9
LNU215
24663.4
9.659
0.016
29.3


LNU27
24873.1
7.237
0.033
15.8
LNU215
24663.3
8.611
0.239
15.2


LNU27
24873.4
6.541
0.520
4.6
LNU215
24661.4
8.607
0.103
15.2


LNU27
24871.4
6.422
0.617
2.7
LNU27
24873.1
11.099
0.106
48.5


LNU44
24923.3
7.316
0.022
17.0
LNU27
24873.4
8.805
0.231
17.8


LNU44
24924.2
7.272
0.018
16.3
LNU44
24924.2
11.478
0.024
53.6


LNU44
24922.3
7.169
0.045
14.7
LNU44
24924.3
10.805
0.114
44.6


LNU54
24901.2
7.648
0.030
22.3
LNU44
24922.3
10.586
0.205
41.7


LNU54
24902.4
7.438
0.010
19.0
LNU44
24923.3
9.478
0.026
26.9


LNU54
24903.5
7.013
0.120
12.2
LNU54
24903.5
12.998
0.000
74.0


LNU54
24903.3
6.934
0.256
10.9
LNU54
24901.2
11.545
0.124
54.5


LNU54
24902.7
6.461
0.603
3.3
LNU54
24903.3
11.231
0.101
50.3


LNU79
24882.2
7.312
0.018
16.9
LNU54
24902.4
9.290
0.026
24.3


LNU79
24881.1
7.287
0.017
16.5
LNU79
24884.4
11.593
0.001
55.2


LNU79
24884.4
7.052
0.043
12.8
LNU79
24881.1
11.528
0.010
54.3


LNU79
24883.2
6.742
0.224
7.8
LNU79
24884.3
9.294
0.179
24.4


CONT.
—
6.833
—
0.0
LNU79
24882.2
8.705
0.104
16.5


LNU109
24892.8
7.289
0.058
6.7
CONT.
—
8.911
—
0.0


LNU109
24891.5
7.107
0.314
4.0
LNU109
24891.5
11.274
0.022
26.5


LNU110
24952.1
8.060
0.001
18.0
LNU109
24892.6
10.560
0.281
18.5


LNU110
24952.3
6.969
0.733
2.0
LNU109
24891.2
9.241
0.800
3.7


LNU133
24744.3
7.713
0.005
12.9
LNU110
24952.1
19.086
0.000
114.2


LNU133
24741.1
7.500
0.051
9.8
LNU110
24952.3
12.178
0.071
36.7


LNU133
24742.2
6.891
0.785
0.8
LNU110
24953.2
10.598
0.334
18.9


LNU19
25151.1
7.230
0.230
5.8
LNU110
24954.3
9.930
0.306
11.4


LNU27
24873.4
7.547
0.015
10.5
LNU133
24744.3
14.915
0.004
67.4


LNU27
24871.4
7.040
0.366
3.0
LNU133
24741.1
12.618
0.097
41.6


LNU44
24922.3
7.636
0.008
11.8
LNU133
24742.2
10.314
0.139
15.7


LNU54
24901.2
7.770
0.003
13.7
LNU133
24741.2
9.795
0.366
9.9


LNU54
24903.5
6.959
0.693
1.9
LNU19
25151.1
12.769
0.053
43.3


LNU6
24992.3
7.592
0.020
11.1
LNU27
24873.4
14.340
0.000
60.9


LNU6
24994.5
7.248
0.156
6.1
LNU44
24922.3
16.486
0.000
85.0


LNU79
24884.4
7.615
0.008
11.4
LNU54
24901.2
14.207
0.007
59.4


LNU79
24881.1
7.315
0.048
7.1
LNU54
24903.5
10.705
0.215
20.1


LNU79
24882.2
6.938
0.704
1.5
LNU6
24992.3
14.206
0.025
59.4


CONT.
—
6.439
—
0.0
LNU6
24994.5
11.407
0.229
28.0


LNU109
24892.8
7.505
0.066
16.6
LNU6
24994.2
9.941
0.232
11.6


LNU109
24891.5
6.844
0.408
6.3
LNU79
24884.4
15.230
0.000
70.9


LNU143
25975.3
7.404
0.086
15.0
LNU79
24881.1
11.673
0.018
31.0


LNU143
25972.1
7.221
0.137
12.1
LNU79
24882.2
10.918
0.273
22.5


LNU143
25971.2
6.746
0.523
4.8
LNU79
24884.3
10.381
0.258
16.5


LNU143
25971.5
6.725
0.555
4.4
LNU79
24883.2
9.300
0.577
4.4


LNU154
14604.7
7.068
0.230
9.8
CONT.
—
6.329
—
0.0


LNU154
14604.6
6.971
0.293
8.3
LNU109
24892.8
12.163
0.002
92.2


LNU154
14601.6
6.857
0.438
6.5
LNU109
24891.5
9.351
0.067
47.8


LNU196
25532.2
7.657
0.044
18.9
LNU109
24891.2
9.220
0.134
45.7


LNU196
25534.1
7.153
0.231
11.1
LNU143
25975.3
8.893
0.016
40.5


LNU196
25533.1
6.825
0.414
6.0
LNU143
25972.1
8.480
0.043
34.0


LNU196
25532.1
6.745
0.628
4.7
LNU143
25975.2
7.944
0.277
25.5


LNU207
24642.5
7.512
0.065
16.7
LNU143
25971.5
7.539
0.396
19.1


LNU207
24644.18
7.441
0.076
15.6
LNU154
14601.6
9.541
0.108
50.8


LNU207
24642.4
7.339
0.109
14.0
LNU154
14604.6
9.238
0.039
46.0


LNU207
24644.13
6.858
0.410
6.5
LNU154
14604.7
8.969
0.032
41.7


LNU288
14564.9
7.527
0.062
16.9
LNU154
14602.8
8.206
0.375
29.7


LNU288
14562.1
7.058
0.217
9.6
LNU196
25532.2
14.062
0.001
122.2


LNU288
14562.7
6.960
0.350
8.1
LNU196
25534.1
11.123
0.166
75.8


LNU288
14562.9
6.822
0.401
5.9
LNU196
25532.1
8.240
0.335
30.2


LNU50
26023.2
7.381
0.093
14.6
LNU196
25531.2
6.924
0.550
9.4


LNU50
26023.5
7.254
0.124
12.7
LNU196
25533.1
6.629
0.666
4.7


LNU50
26024.2
7.129
0.249
10.7
LNU207
24642.4
11.713
0.059
85.1


LNU50
26025.3
6.750
0.499
4.8
LNU207
24642.5
10.786
0.013
70.4


LNU52
25723.2
7.754
0.034
20.4
LNU207
24644.18
9.470
0.036
49.6


LNU52
25721.4
7.203
0.191
11.9
LNU207
24644.13
8.136
0.302
28.6


LNU52
25723.1
6.913
0.377
7.3
LNU288
14562.1
8.997
0.162
42.2


LNU52
25721.3
6.720
0.614
4.4
LNU288
14562.7
8.395
0.113
32.6


CONT.
—
5.633
—
0.0
LNU288
14564.9
8.299
0.036
31.1


LNU143
25975.2
6.753
0.015
19.9
LNU288
14562.12
7.258
0.206
14.7


LNU143
25975.3
6.608
0.003
17.3
LNU288
14562.9
7.135
0.346
12.7


LNU143
25971.5
6.566
0.020
16.6
LNU50
26023.2
9.476
0.034
49.7


LNU143
25972.1
6.543
0.041
16.1
LNU50
26024.2
9.283
0.125
46.7


LNU143
25971.2
6.461
0.006
14.7
LNU50
26025.4
8.172
0.053
29.1


LNU154
14602.8
6.492
0.016
15.2
LNU50
26023.5
7.519
0.204
18.8


LNU154
14601.6
6.149
0.108
9.2
LNU50
26022.1
6.843
0.599
8.1


LNU207
24642.5
6.965
0.011
23.6
LNU52
25723.2
14.598
0.005
130.7


LNU207
24641.1
6.872
0.016
22.0
LNU52
25721.4
10.374
0.100
63.9


LNU207
24642.4
6.413
0.011
13.8
LNU52
25721.3
10.089
0.085
59.4


LNU211
24771.1
7.033
0.007
24.8
LNU52
25723.1
7.096
0.545
12.1


LNU211
24771.3
6.030
0.095
7.0
CONT.
—
8.555
—
0.0


LNU211
24774.4
5.978
0.569
6.1
LNU143
25975.2
9.977
0.289
16.6


LNU52
25723.1
7.106
0.004
26.1
LNU143
25975.3
9.097
0.708
6.3


LNU52
25721.2
6.927
0.005
23.0
LNU154
14602.8
10.155
0.142
18.7


LNU52
25721.1
6.506
0.021
15.5
LNU207
24642.5
11.122
0.079
30.0


LNU52
25723.2
5.812
0.660
3.2
LNU207
24641.1
10.558
0.138
23.4


LNU69
14571.1
7.049
0.003
25.1
LNU207
24642.4
9.609
0.289
12.3


LNU69
14573.3
6.658
0.008
18.2
LNU211
24771.1
9.940
0.183
16.2


LNU69
14572.9
6.285
0.020
11.6
LNU52
25721.1
12.111
0.083
41.6


LNU69
14572.8
6.259
0.025
11.1
LNU52
25723.1
11.473
0.179
34.1


CONT.
—
5.748
—
0.0
LNU52
25721.2
11.153
0.205
30.4


LNU150
24842.9
7.210
0.000
25.4
LNU52
25723.2
10.026
0.431
17.2


LNU150
24841.9
7.204
0.001
25.3
LNU69
14571.1
11.815
0.106
38.1


LNU150
24843.5
6.754
0.062
17.5
LNU69
14572.9
9.516
0.423
11.2


LNU179
24632.5
7.693
0.000
33.8
LNU69
14572.8
9.471
0.279
10.7


LNU179
24631.9
7.481
0.004
30.1
CONT.
—
7.365
—
0.0


LNU179
24631.6
6.733
0.008
17.1
LNU150
24842.9
13.108
0.000
78.0


LNU179
24631.7
6.139
0.210
6.8
LNU150
24841.9
9.862
0.067
33.9


LNU232
26003.3
6.837
0.002
18.9
LNU150
24843.5
9.813
0.252
33.2


LNU232
26001.5
6.830
0.057
18.8
LNU179
24632.5
12.877
0.028
74.8


LNU232
26003.7
6.060
0.359
5.4
LNU179
24631.9
10.523
0.117
42.9


LNU232
26001.2
6.004
0.436
4.5
LNU179
24631.6
7.714
0.754
4.7


LNU235
26184.4
7.751
0.000
34.8
LNU232
26003.7
9.269
0.098
25.9


LNU235
26185.3
7.381
0.005
28.4
LNU232
26003.3
7.728
0.720
4.9


LNU235
26182.1
6.699
0.017
16.5
LNU235
26184.4
13.577
0.001
84.3


LNU235
26184.2
6.546
0.026
13.9
LNU235
26185.3
11.955
0.064
62.3


LNU235
26185.2
6.094
0.276
6.0
LNU235
26182.1
8.994
0.179
22.1


LNU242
25474.1
7.362
0.001
28.1
LNU235
26184.2
8.691
0.167
18.0


LNU242
25473.1
7.108
0.003
23.7
LNU242
25473.1
12.344
0.000
67.6


LNU242
25473.3
6.390
0.073
11.2
LNU242
25474.1
10.746
0.086
45.9


LNU242
25471.1
6.244
0.148
8.6
LNU76
26425.1
11.223
0.020
52.4


LNU242
25472.1
5.879
0.763
2.3
LNU76
26421.2
10.070
0.047
36.7


LNU76
26425.1
7.027
0.001
22.2
LNU76
26423.1
9.805
0.046
33.1


LNU76
26423.1
6.864
0.017
19.4
LNU76
26421.1
8.691
0.466
18.0


LNU76
26421.2
6.813
0.066
18.5
LNU95
13985.11
12.944
0.033
75.7


LNU76
26421.1
6.590
0.106
14.6
LNU95
13985.15
9.432
0.058
28.1


LNU95
13985.11
7.431
0.000
29.3
LNU95
13985.12
9.399
0.182
27.6


LNU95
13985.19
7.114
0.004
23.8
LNU95
13985.19
9.281
0.368
26.0


LNU95
13985.15
6.928
0.001
20.5
LNU95
13985.16
7.914
0.569
7.5


LNU95
13985.12
6.791
0.036
18.1
CONT.
—
9.009
—
0.0


LNU95
13985.16
6.612
0.048
15.0
LNU232
26001.5
11.019
0.177
22.3


CONT.
—
6.930
—
0.0
LNU235
26184.4
14.496
0.000
60.9


LNU179
24631.6
7.162
0.374
3.4
LNU235
26185.2
14.127
0.017
56.8


LNU232
26001.5
7.624
0.145
10.0
LNU235
26184.2
10.838
0.207
20.3


LNU232
26003.3
7.555
0.005
9.0
LNU242
25474.1
10.306
0.406
14.4


LNU235
26184.4
8.109
0.000
17.0
LNU288
14563.9
12.963
0.135
43.9


LNU235
26184.2
7.760
0.006
12.0
LNU288
14562.1
10.884
0.044
20.8


LNU235
26185.2
7.656
0.031
10.5
LNU288
14564.9
10.080
0.495
11.9


LNU242
25474.1
7.198
0.532
3.9
LNU76
26421.2
11.857
0.344
31.6


LNU288
14564.9
7.294
0.201
5.3
LNU76
26422.2
9.715
0.723
7.8


LNU288
14562.7
7.126
0.571
2.8
LNU76
26423.1
9.480
0.777
5.2


LNU288
14563.6
7.066
0.547
2.0
LNU95
13985.16
13.851
0.037
53.7


LNU288
14563.9
7.050
0.623
1.7
LNU95
13985.12
12.566
0.179
39.5


LNU76
26421.2
7.262
0.303
4.8
LNU95
13985.15
12.253
0.037
36.0


LNU95
13985.16
7.835
0.000
13.1
CONT.
—
7.303
—
0.0


LNU95
13985.15
7.095
0.521
2.4
LNU101
27632.7
10.044
0.030
37.5


CONT.
—
6.277
—
0.0
LNU101
27635.1
8.226
0.494
12.6


LNU101
27632.7
7.595
0.000
21.0
LNU101
27632.1
8.137
0.145
11.4


LNU101
27635.1
6.518
0.421
3.8
LNU128
26515.3
12.337
0.000
68.9


LNU128
26515.3
7.668
0.001
22.2
LNU128
26511.5
9.896
0.028
35.5


LNU128
26511.5
7.477
0.000
19.1
LNU128
26515.2
8.997
0.171
23.2


LNU128
26515.2
6.784
0.237
8.1
LNU128
26511.4
7.501
0.709
2.7


LNU192
28315.2
7.732
0.000
23.2
LNU192
28315.2
11.544
0.001
58.1


LNU192
28313.2
7.474
0.001
19.1
LNU192
28313.2
10.093
0.000
38.2


LNU192
28312.2
6.813
0.031
8.5
LNU192
28313.3
8.887
0.209
21.7


LNU192
28313.3
6.626
0.429
5.6
LNU192
28312.2
7.667
0.532
5.0


LNU206
27621.2
6.916
0.023
10.2
LNU206
27621.2
10.347
0.011
41.7


LNU206
27622.1
6.838
0.191
8.9
LNU206
27621.1
8.702
0.012
19.2


LNU211
24771.1
7.325
0.008
16.7
LNU206
27622.1
8.138
0.108
11.4


LNU282
27562.1
7.580
0.017
20.8
LNU211
24771.1
11.295
0.035
54.7


LNU282
27563.1
7.388
0.005
17.7
LNU211
24773.2
9.318
0.074
27.6


LNU282
27563.3
7.299
0.045
16.3
LNU282
27562.1
12.185
0.026
66.8


LNU282
27565.2
6.768
0.224
7.8
LNU282
27563.3
10.979
0.012
50.3


LNU69
14571.1
7.603
0.000
21.1
LNU282
27563.1
10.436
0.037
42.9


LNU69
14573.5
6.811
0.135
8.5
LNU282
27565.2
8.007
0.266
9.6


LNU75
27572.1
7.450
0.000
18.7
LNU69
14571.1
12.531
0.002
71.6


LNU75
27572.2
7.264
0.069
15.7
LNU69
14573.5
9.289
0.077
27.2


LNU75
27571.4
6.677
0.333
6.4
LNU75
27572.2
11.342
0.094
55.3


LNU75
27572.3
6.590
0.407
5.0
LNU75
27572.1
9.625
0.022
31.8


CONT.
—
5.813
—
0.0
LNU75
27572.3
8.378
0.398
14.7


LNU101
27632.5
6.807
0.016
17.1
LNU75
27571.4
7.934
0.591
8.6


LNU101
27632.6
6.150
0.322
5.8
CONT.
—
6.735
—
0.0


LNU118
14012.15
5.962
0.626
2.6
LNU101
27632.5
8.284
0.190
23.0


LNU128
26515.3
6.716
0.035
15.5
LNU118
14012.15
8.399
0.071
24.7


LNU128
26511.5
6.226
0.315
7.1
LNU206
27621.2
12.586
0.032
86.9


LNU206
27621.2
7.622
0.001
31.1
LNU206
27622.4
11.079
0.012
64.5


LNU206
27622.4
7.043
0.014
21.2
LNU206
27622.1
10.117
0.052
50.2


LNU206
27622.1
6.757
0.103
16.2
LNU206
27621.1
9.288
0.118
37.9


LNU249
26152.4
6.759
0.189
16.3
LNU249
26153.1
10.203
0.102
51.5


LNU249
26153.1
6.492
0.102
11.7
LNU249
26154.2
9.330
0.208
38.5


LNU249
26151.1
6.359
0.283
9.4
LNU249
26152.4
8.642
0.232
28.3


LNU249
26154.2
6.342
0.316
9.1
LNU282
27563.1
10.545
0.026
56.6


LNU249
26152.2
6.191
0.197
6.5
LNU282
27565.2
10.509
0.072
56.0


LNU282
27565.2
7.232
0.001
24.4
LNU282
27562.1
7.008
0.765
4.1


LNU282
27563.1
6.899
0.017
18.7
LNU288
14563.9
12.005
0.018
78.3


LNU282
27562.1
6.530
0.069
12.3
LNU288
14563.6
9.606
0.173
42.6


LNU282
27565.1
6.410
0.188
10.3
LNU288
14564.8
9.604
0.090
42.6


LNU282
27563.3
6.409
0.205
10.2
LNU288
14562.9
9.080
0.007
34.8


LNU288
14563.6
6.954
0.003
19.6
LNU288
14562.7
8.511
0.202
26.4


LNU288
14562.7
6.914
0.014
18.9
LNU75
27572.2
13.524
0.039
100.8


LNU288
14563.9
6.862
0.016
18.0
LNU75
27571.2
11.853
0.022
76.0


LNU288
14562.9
6.355
0.078
9.3
LNU75
27572.3
11.191
0.014
66.2


LNU288
14564.8
6.291
0.244
8.2
LNU75
27571.4
9.627
0.064
42.9


LNU75
27572.2
7.464
0.003
28.4
CONT.
—
11.156
—
0.0


LNU75
27571.2
7.428
0.000
27.8
LNU112
28212.4
12.419
0.271
11.3


LNU75
27571.4
7.346
0.000
26.4
LNU183
24865.1
12.047
0.600
8.0


LNU75
27572.3
7.114
0.001
22.4
LNU183
24863.1
11.863
0.548
6.3


CONT.
—
6.748
—
0.0
LNU201
28222.2
12.100
0.653
8.5


LNU11
28205.2
7.162
0.194
6.1
LNU201
28223.1
11.563
0.780
3.6


LNU112
28212.4
7.424
0.065
10.0
LNU268
26044.2
13.230
0.270
18.6


LNU14
27821.3
7.205
0.162
6.8
CONT.
—
8.642
—
0.0


LNU14
27823.2
6.881
0.653
2.0
LNU11
28205.2
11.985
0.010
38.7


LNU14
27824.2
6.880
0.664
2.0
LNU11
28203.2
11.222
0.219
29.9


LNU14
27821.1
6.873
0.710
1.8
LNU11
28205.1
11.099
0.083
28.4


LNU201
28223.3
7.200
0.199
6.7
LNU11
28202.5
11.048
0.171
27.8


LNU201
28222.2
7.189
0.180
6.5
LNU11
28204.1
9.232
0.632
6.8


LNU201
28223.1
7.123
0.287
5.6
LNU112
28212.1
11.083
0.103
28.3


LNU201
28222.3
7.105
0.263
5.3
LNU112
28212.4
9.361
0.431
8.3


LNU268
26044.2
7.215
0.173
6.9
LNU14
27821.3
14.480
0.003
67.6


LNU268
26041.6
7.074
0.272
4.8
LNU14
27821.4
11.236
0.153
30.0


CONT.
—
6.558
—
0.0
LNU14
27824.2
9.792
0.285
13.3


LNU11
28205.2
7.549
0.054
15.1
LNU14
27821.1
9.067
0.556
4.9


LNU11
28202.5
7.066
0.043
7.7
LNU183
24864.6
16.882
0.000
95.4


LNU11
28205.1
6.967
0.082
6.2
LNU183
24865.1
13.996
0.010
62.0


LNU11
28203.2
6.673
0.705
1.8
LNU183
24863.12
13.932
0.002
61.2


LNU112
28212.1
7.029
0.091
7.2
LNU183
24863.1
11.228
0.071
29.9


LNU112
28212.4
6.855
0.357
4.5
LNU191
28325.4
11.845
0.144
37.1


LNU14
27821.3
7.453
0.004
13.6
LNU191
28323.1
11.545
0.092
33.6


LNU14
27821.4
7.112
0.086
8.4
LNU191
28321.3
10.102
0.059
16.9


LNU14
27824.2
6.705
0.664
2.2
LNU191
28324.2
9.088
0.641
5.2


LNU14
27821.1
6.617
0.785
0.9
LNU201
28222.2
13.603
0.012
57.4


LNU183
24864.6
7.736
0.001
18.0
LNU201
28223.3
12.364
0.053
43.1


LNU183
24863.12
7.306
0.109
11.4
LNU201
28221.3
9.939
0.105
15.0


LNU183
24863.1
6.908
0.265
5.3
LNU201
28223.1
9.431
0.624
9.1


LNU183
24865.1
6.901
0.514
5.2
LNU268
26041.4
10.798
0.011
25.0


LNU201
28222.2
7.686
0.001
17.2
LNU268
26041.6
10.549
0.191
22.1


LNU201
28223.3
7.225
0.075
10.2
LNU268
26043.4
10.366
0.163
20.0


LNU201
28223.1
6.881
0.350
4.9
CONT.
—
8.826
—
0.0


LNU268
26041.6
7.361
0.030
12.3
LNU107
14583.8
12.388
0.135
40.4


LNU268
26041.4
7.076
0.043
7.9
LNU107
14585.5
10.429
0.192
18.2


CONT.
—
6.688
—
0.0
LNU107
14584.9
9.576
0.645
8.5


LNU107
14585.5
6.948
0.465
3.9
LNU116
14492.9
12.023
0.153
36.2


LNU107
14584.9
6.902
0.582
3.2
LNU116
14494.5
11.609
0.162
31.5


LNU107
14583.8
6.876
0.677
2.8
LNU116
14492.5
10.826
0.224
22.7


LNU121
27711.1
7.515
0.022
12.4
LNU121
27713.4
13.101
0.024
48.4


LNU121
27713.4
7.131
0.040
6.6
LNU121
25642.2
12.536
0.016
42.0


LNU121
25642.2
6.832
0.534
2.2
LNU121
27711.1
12.183
0.025
38.0


LNU126
25345.1
7.223
0.030
8.0
LNU121
27713.1
9.357
0.718
6.0


LNU126
25343.1
7.063
0.039
5.6
LNU126
25345.1
12.082
0.186
36.9


LNU126
25343.3
6.914
0.580
3.4
LNU126
25343.1
11.624
0.066
31.7


LNU158
27433.3
7.724
0.001
15.5
LNU126
25343.3
10.159
0.512
15.1


LNU158
27433.2
7.263
0.050
8.6
LNU158
27433.3
13.205
0.003
49.6


LNU158
27432.5
7.064
0.448
5.6
LNU158
27432.5
13.036
0.262
47.7


LNU177
24764.9
7.526
0.199
12.5
LNU158
27433.2
11.539
0.083
30.7


LNU177
24762.6
7.459
0.001
11.5
LNU177
24762.6
13.660
0.000
54.8


LNU177
24764.12
7.090
0.429
6.0
LNU177
24764.9
10.753
0.487
21.8


LNU177
24765.2
6.840
0.490
2.3
LNU177
24765.2
9.467
0.570
7.3


LNU182
25384.1
7.669
0.032
14.7
LNU182
25384.1
14.203
0.000
60.9


LNU182
27521.4
7.285
0.044
8.9
LNU182
27521.4
12.084
0.082
36.9


LNU182
25384.5
6.920
0.233
3.5
LNU182
25384.2
9.950
0.536
12.7


LNU2
27842.1
7.230
0.111
8.1
LNU182
25384.5
9.279
0.725
5.1


LNU2
27842.3
6.891
0.222
3.0
LNU2
27842.1
10.901
0.082
23.5


LNU225
25991.5
7.854
0.000
17.4
LNU2
27842.3
10.146
0.166
15.0


LNU225
25991.3
7.318
0.004
9.4
LNU225
25991.5
16.802
0.002
90.4


LNU225
25991.2
7.306
0.164
9.2
LNU225
25991.2
13.379
0.239
51.6


LNU239
26284.1
7.073
0.262
5.8
LNU239
26284.1
10.137
0.231
14.9


LNU57
27852.1
7.119
0.122
6.5
LNU57
27852.1
9.278
0.731
5.1


LNU83
27681.4
7.284
0.166
8.9
LNU83
27681.4
10.944
0.212
24.0


LNU83
27682.1
6.944
0.535
3.8
LNU83
27684.1
9.726
0.592
10.2


CONT.
—
6.610
—
0.0
CONT.
—
8.649
—
0.0


LNU107
14584.9
7.452
0.011
12.7
LNU107
14584.9
13.777
0.006
59.3


LNU116
14492.5
6.844
0.603
3.5
LNU107
14583.8
10.320
0.189
19.3


LNU121
27711.1
7.402
0.015
12.0
LNU107
14585.2
9.003
0.660
4.1


LNU121
27713.1
7.140
0.136
8.0
LNU116
14492.5
12.926
0.175
49.5


LNU121
27713.4
6.865
0.366
3.9
LNU116
14494.5
11.992
0.193
38.6


LNU121
25642.2
6.793
0.733
2.8
LNU116
14493.6
9.901
0.185
14.5


LNU126
25343.1
7.627
0.015
15.4
LNU121
25642.2
12.625
0.067
46.0


LNU126
25345.1
6.880
0.247
4.1
LNU121
27711.1
12.159
0.059
40.6


LNU126
25343.3
6.774
0.579
2.5
LNU121
27713.1
11.980
0.126
38.5


LNU126
25343.4
6.743
0.602
2.0
LNU121
27713.4
10.723
0.212
24.0


LNU158
27433.3
7.700
0.001
16.5
LNU126
25343.1
12.591
0.061
45.6


LNU158
27432.5
7.579
0.003
14.7
LNU126
25343.3
9.454
0.636
9.3


LNU158
27433.2
7.218
0.174
9.2
LNU126
25343.4
9.346
0.489
8.1


LNU158
27434.5
7.076
0.202
7.1
LNU158
27433.3
14.173
0.003
63.9


LNU177
24764.12
7.403
0.005
12.0
LNU158
27432.5
12.764
0.002
47.6


LNU177
24765.2
7.050
0.223
6.7
LNU158
27433.2
10.182
0.303
17.7


LNU177
24763.6
6.895
0.320
4.3
LNU177
24764.12
12.256
0.002
41.7


LNU182
27521.4
7.048
0.183
6.6
LNU177
24763.6
9.330
0.473
7.9


LNU182
25384.5
6.953
0.377
5.2
LNU177
24762.6
9.229
0.375
6.7


LNU182
25384.2
6.938
0.392
5.0
LNU182
25384.6
9.479
0.435
9.6


LNU182
25384.6
6.861
0.470
3.8
LNU182
25384.1
9.407
0.565
8.8


LNU2
27842.1
7.658
0.002
15.9
LNU182
25384.5
9.310
0.530
7.6


LNU2
27842.3
7.291
0.010
10.3
LNU2
27842.1
12.557
0.031
45.2


LNU2
25713.1
6.781
0.432
2.6
LNU2
27842.3
10.135
0.121
17.2


LNU225
25991.3
7.597
0.009
14.9
LNU2
25713.1
9.741
0.417
12.6


LNU225
25991.1
7.436
0.021
12.5
LNU225
25991.3
14.898
0.000
72.2


LNU225
25991.2
7.232
0.026
9.4
LNU225
25991.2
13.446
0.031
55.5


LNU225
25991.8
7.229
0.041
9.4
LNU225
25991.8
11.180
0.011
29.3


LNU225
25991.5
7.188
0.033
8.7
LNU225
25991.1
10.790
0.067
24.8


LNU239
26284.2
7.240
0.074
9.5
LNU239
26284.2
11.555
0.003
33.6


LNU239
26281.1
7.010
0.093
6.1
LNU239
26281.1
8.866
0.720
2.5


LNU239
26284.1
6.697
0.763
1.3
LNU57
27852.1
13.264
0.000
53.4


LNU57
27852.1
7.461
0.005
12.9
LNU57
27851.2
12.792
0.028
47.9


LNU57
27851.2
7.231
0.067
9.4
LNU57
27854.5
10.651
0.061
23.1


LNU57
27854.3
7.077
0.107
7.1
LNU57
27854.3
9.037
0.488
4.5


LNU57
27854.5
7.063
0.150
6.8
LNU83
27685.1
14.008
0.002
62.0


LNU83
27685.2
7.614
0.010
15.2
LNU83
27685.2
11.664
0.041
34.9


LNU83
27685.1
7.587
0.001
14.8
LNU83
27681.4
11.522
0.020
33.2


LNU83
27681.4
7.289
0.017
10.3
CONT.
—
10.064
—



LNU83
27682.1
7.010
0.337
6.0
LNU17
13991.1
11.488
0.32
14.1


CONT.
—
6.95
—
0.0
CONT.
12033.3
6.301

0.0


LNU17
13991.1
7.33
0.24
5.3
LNU129
27501.2
10.049
<0.05
59.5


LNU17
13991.14
7.11
0.62
2.1
LNU129
27502.4
8.465
<0.05
34.3


CONT.
—
6.181
—
0.0
LNU129
27503.4
8.035
<0.05
27.5


LNU129
27501.2
7.291
<0.1
17.9
LNU129
27504.2
9.203
<0.05
46.0


LNU129
27502.4
6.724
<0.1
8.8
LNU129
27504.3
10.684
<0.05
69.6


LNU129
27503.4
6.617
<0.2
7.1
LNU147
27511.4
8.137
<0.05
29.1


LNU129
27504.3
7.254
<0.1
17.4
LNU147
27512.1
7.206

14.4


LNU147
27511.4
6.549
<0.5
5.9
LNU147
27513.2
9.441
<0.05
49.8


LNU147
27512.1
6.521
<0.5
5.5
LNU147
27514.1
8.236
<0.05
30.7


LNU147
27514.2
6.686
<0.1
8.2
LNU147
27514.2
9.705
<0.05
54.0


LNU189
26382.4
7.221
<0.1
16.8
LNU153
24851.3
7.510

19.2


LNU189
26383.1
6.520
<0.5
5.5
LNU153
24851.4
7.033

11.6


LNU189
26385.1
6.833
<0.1
10.5
LNU189
26382.3
11.910
<0.05
89.0


LNU219
27462.1
7.278
<0.1
17.7
LNU189
26382.4
12.085
<0.05
91.8


LNU219
27462.2
6.981
<0.1
12.9
LNU189
26383.1
9.119
<0.05
44.7


LNU256
26211.2
6.754
<0.1
9.3
LNU189
26385.1
10.337
<0.05
64.0


LNU256
26212.3
6.691
<0.1
8.2
LNU219
27461.1
9.304
<0.05
47.7


LNU256
26214.1
7.064
<0.1
14.3
LNU219
27462.1
14.273
<0.05
126.5


LNU257
26254.1
7.230
<0.1
17.0
LNU219
27462.2
11.156
<0.05
77.1


LNU257
26254.3
7.714
<0.1
24.8
LNU219
27464.2
7.151

13.5


LNU257
26255.2
6.760
<0.1
9.4
LNU256
26211.2
7.359

16.8


LNU257
26255.3
6.777
<0.1
9.6
LNU256
26212.3
10.694
<0.05
69.7


LNU261
27401.4
7.082
<0.1
14.6
LNU256
26213.1
9.008
<0.05
43.0


LNU261
27403.2
6.719
<0.1
8.7
LNU256
26214.1
11.063
<0.05
75.6


LNU261
27405.1
6.970
<0.1
12.8
LNU257
26254.1
9.639
<0.05
53.0


LNU261
27405.3
6.748
<0.1
9.2
LNU257
26254.3
15.465
<0.05
145.4


LNU33
25552.2
6.847
<0.1
10.8
LNU257
26254.7
9.843
<0.05
56.2


LNU33
25553.3
6.888
<0.1
11.4
LNU257
26255.2
6.589
<0.8
4.6


LNU35
27421.2
6.636
<0.2
7.4
LNU257
26255.3
7.862
<0.2
24.8


LNU35
27423.3
6.966
<0.1
12.7
LNU261
27401.4
8.612
<0.05
36.7


LNU35
27424.3
6.633
<0.2
7.3
LNU261
27403.2
9.379
<0.05
48.8


LNU50
26022.1
6.629
<0.2
7.2
LNU261
27405.1
8.806
<0.05
39.8


LNU50
26023.2
6.948
<0.1
12.4
LNU261
27405.3
8.021
<0.2
27.3


LNU50
26023.3
6.821
<0.1
10.3
LNU33
25552.2
9.471
<0.05
50.3


LNU50
26025.3
7.624
<0.1
23.3
LNU33
25553.2
7.380
<0.4
17.1


LNU70
25311.4
7.590
<0.1
22.8
LNU33
25553.3
7.792
<0.2
23.7


LNU70
25313.2
6.816
<0.1
10.3
LNU33
25555.1
7.261
<0.4
15.2







LNU35
27421.2
8.163
<0.2
29.5







LNU35
27422.1
8.300
<0.05
31.7







LNU35
27423.3
11.263
<0.05
78.7







LNU35
27424.3
8.953
<0.05
42.1







LNU35
27424.4
8.455
<0.05
34.2







LNU50
26022.1
10.653
<0.05
69.1







LNU50
26023.2
12.657
<0.05
100.9







LNU50
26023.3
7.219
<0.5
14.6







LNU50
26024.1
7.889
<0.2
25.2







LNU50
26025.3
15.671
<0.05
148.7







LNU70
25311.4
14.371
<0.05
128.1







LNU70
25313.1
7.720
<0.1
22.5







LNU70
25313.2
10.792
<0.05
71.3





Table 64.


“CONT.”—Control;


“Ave.”—Average;


“% Incr.” = % increment.













TABLE 65







Genes showing improved root performance at nitrogen deficient conditions (T1 generation)











Roots Length [cm]

Roots Coverage [cm2]
















Gene
Time

p-
%
Gene
Time

p-
%


Name
Point
Ave.
value
incr.
Name
Point
Ave.
value
incr.



















CONT.
Roots
0.252
—
0.0
CONT.
Roots
0.017
—
0.0



Length




Coverage






TP1




TP1





LNU107
Roots
0.477
0.000
89.1
LNU107
Roots
0.052
0.011
212.2



Length




Coverage






TP1




TP1





LNU118
Roots
0.338
0.000
33.8
LNU118
Roots
0.039
0.001
138.7



Length




Coverage






TP1




TP1





LNU121
Roots
0.492
0.004
95.1
LNU121
Roots
0.083
0.010
400.7



Length




Coverage






TP1




TP1





LNU141
Roots
0.435
0.020
72.4
LNU141
Roots
0.042
0.103
155.4



Length




Coverage






TP1




TP1





LNU150
Roots
0.355
0.024
40.5
LNU150
Roots
0.042
0.005
153.3



Length




Coverage






TP1




TP1





LNU154
Roots
0.439
0.001
74.0
LNU154
Roots
0.066
0.012
300.8



Length




Coverage






TP1




TP1





LNU210
Roots
0.401
0.001
59.0
LNU210
Roots
0.053
0.009
218.2



Length




Coverage






TP1




TP1





LNU68
Roots
0.403
0.038
59.7
LNU68
Roots
0.054
0.066
227.3



Length




Coverage






TP1




TP1





CONT.
Roots
2.097
—
0.0
CONT.
Roots
1.275
—
0.0



Length




Coverage






TP2




TP2





LNU107
Roots
2.164
0.741
3.2
LNU121
Roots
1.928
0.021
51.2



Length




Coverage






TP2




TP2





LNU121
Roots
2.638
0.033
25.8
LNU150
Roots
1.350
0.667
5.8



Length




Coverage






TP2




TP2





LNU150
Roots
2.296
0.318
9.5
LNU154
Roots
1.296
0.891
1.6



Length




Coverage






TP2




TP2





LNU154
Roots
2.493
0.015
18.9
LNU210
Roots
1.289
0.935
1.1



Length




Coverage






TP2




TP2





LNU210
Roots
2.280
0.244
8.7
LNU68
Roots
1.350
0.607
5.9



Length




Coverage






TP2




TP2





LNU68
Roots
2.524
0.010
20.4
CONT.
Roots
3.537
—
0.0



Length




Coverage






TP2




TP3





CONT.
Roots
4.072
—
0.0
LNU121
Roots
5.046
0.001
42.7



Length




Coverage






TP3




TP3





LNU121
Roots
4.586
0.100
12.6
LNU150
Roots
3.751
0.574
6.0



Length




Coverage






TP3




TP3





LNU154
Roots
4.448
0.335
9.2
LNU154
Roots
3.718
0.766
5.1



Length




Coverage






TP3




TP3





LNU210
Roots
4.153
0.766
2.0
CONT.
Roots
0.045
—
0.0



Length




Coverage






TP3




TP1





LNU68
Roots
4.387
0.324
7.7
LNU127
Roots
0.119
0.184
164.9



Length




Coverage






TP3




TP1





CONT.
Roots
0.324
—
0.0
LNU188
Roots
0.051
0.521
14.3



Length




Coverage






TP1




TP1





LNU127
Roots
0.561
0.073
73.0
LNU2
Roots
0.090
0.023
100.2



Length




Coverage






TP1




TP1





LNU188
Roots
0.423
0.046
30.3
LNU239
Roots
0.076
0.002
69.8



Length




Coverage






TP1




TP1





LNU2
Roots
0.518
0.002
59.7
LNU255
Roots
0.063
0.180
39.0



Length




Coverage






TP1




TP1





LNU239
Roots
0.512
0.000
57.7
LNU265
Roots
0.089
0.026
97.7



Length




Coverage






TP1




TP1





LNU255
Roots
0.496
0.005
53.0
LNU275
Roots
0.131
0.003
192.0



Length




Coverage






TP1




TP1





LNU265
Roots
0.455
0.003
40.4
LNU32
Roots
0.074
0.017
64.8



Length




Coverage






TP1




TP1





LNU275
Roots
0.552
0.000
70.0
LNU57
Roots
0.092
0.001
105.0



Length




Coverage






TP1




TP1





LNU32
Roots
0.525
0.162
61.7
LNU58
Roots
0.096
0.012
112.6



Length




Coverage






TP1




TP1





LNU57
Roots
0.493
0.000
51.9
LNU83
Roots
0.121
0.001
168.7



Length




Coverage






TP1




TP1





LNU58
Roots
0.509
0.001
56.8
CONT.
Roots
1.456
—
0.0



Length




Coverage






TP1




TP2





LNU83
Roots
0.563
0.006
73.5
LNU127
Roots
1.703
0.344
16.9



Length




Coverage






TP1




TP2





CONT.
Roots
2.393
—
0.0
LNU188
Roots
1.489
0.877
2.3



Length




Coverage






TP2




TP2





LNU127
Roots
2.701
0.092
12.9
LNU2
Roots
1.535
0.657
5.4



Length




Coverage






TP2




TP2





LNU265
Roots
2.427
0.804
1.4
LNU239
Roots
1.488
0.894
2.2



Length




Coverage






TP2




TP2





LNU275
Roots
2.955
0.012
23.5
LNU265
Roots
1.738
0.138
19.4



Length




Coverage






TP2




TP2





LNU57
Roots
2.718
0.057
13.6
LNU275
Roots
3.354
0.000
130.4



Length




Coverage






TP2




TP2





LNU58
Roots
2.980
0.007
24.6
LNU57
Roots
1.990
0.088
36.6



Length




Coverage






TP2




TP2





LNU83
Roots
2.943
0.041
23.0
LNU58
Roots
2.071
0.130
42.2



Length




Coverage






TP2




TP2





CONT.
Roots
4.661
—
0.0
LNU83
Roots
2.185
0.081
50.1



Length




Coverage






TP3




TP2





LNU127
Roots
4.923
0.369
5.6
CONT.
Roots
4.725
—
0.0



Length




Coverage






TP3




TP3





LNU217
Roots
4.893
0.607
5.0
LNU127
Roots
5.855
0.232
23.9



Length




Coverage






TP3




TP3





LNU265
Roots
4.843
0.629
3.9
LNU188
Roots
5.162
0.605
9.2



Length




Coverage






TP3




TP3





LNU275
Roots
5.711
0.030
22.5
LNU217
Roots
4.962
0.782
5.0



Length




Coverage






TP3




TP3





LNU57
Roots
5.252
0.111
12.7
LNU239
Roots
5.289
0.707
11.9



Length




Coverage






TP3




TP3





LNU58
Roots
6.931
0.000
48.7
LNU265
Roots
6.215
0.141
31.5



Length




Coverage






TP3




TP3





LNU83
Roots
5.573
0.047
19.6
LNU275
Roots
11.302
0.000
139.2



Length




Coverage






TP3




TP3





CONT.
Roots
0.251
—
0.0
LNU32
Roots
5.220
0.272
10.5



Length




Coverage






TP1




TP3





LNU176
Roots
0.377
0.004
50.3
LNU57
Roots
5.808
0.095
22.9



Length




Coverage






TP1




TP3





LNU186
Roots
0.387
0.000
54.2
LNU58
Roots
11.015
0.002
133.1



Length




Coverage






TP1




TP3





LNU187
Roots
0.469
0.000
87.1
LNU83
Roots
6.154
0.195
30.2



Length




Coverage






TP1




TP3





LNU214
Roots
0.455
0.005
81.4
CONT.
Roots
0.018
—
0.0



Length




Coverage






TP1




TP1





LNU223
Roots
0.422
0.000
68.4
LNU176
Roots
0.047
0.000
161.9



Length




Coverage






TP1




TP1





LNU233
Roots
0.416
0.000
66.1
LNU186
Roots
0.038
0.024
111.9



Length




Coverage






TP1




TP1





LNU245
Roots
0.432
0.000
72.3
LNU187
Roots
0.036
0.065
102.1



Length




Coverage






TP1




TP1





LNU247
Roots
0.391
0.049
56.0
LNU214
Roots
0.055
0.003
207.0



Length




Coverage






TP1




TP1





LNU251
Roots
0.483
0.026
92.8
LNU223
Roots
0.053
0.008
192.3



Length




Coverage






TP1




TP1





LNU284
Roots
0.302
0.089
20.5
LNU233
Roots
0.055
0.001
205.8



Length




Coverage






TP1




TP1





LNU289
Roots
0.541
0.000
115.9
LNU245
Roots
0.052
0.005
190.1



Length




Coverage






TP1




TP1





LNU70
Roots
0.346
0.027
38.0
LNU247
Roots
0.058
0.010
221.7



Length




Coverage






TP1




TP1





LNU85
Roots
0.515
0.001
105.6
LNU251
Roots
0.048
0.011
167.7



Length




Coverage






TP1




TP1





LNU86
Roots
0.432
0.000
72.3
LNU284
Roots
0.025
0.241
36.0



Length




Coverage






TP1




TP1





CONT.
Roots
2.217
—
0.0
LNU289
Roots
0.064
0.000
253.0



Length




Coverage






TP2




TP1





LNU176
Roots
2.309
0.686
4.2
LNU70
Roots
0.042
0.010
132.3



Length




Coverage






TP2




TP1





LNU214
Roots
2.541
0.142
14.6
LNU85
Roots
0.067
0.000
273.2



Length




Coverage






TP2




TP1





LNU223
Roots
2.466
0.095
11.2
LNU86
Roots
0.066
0.005
266.8



Length




Coverage






TP2




TP1





LNU233
Roots
2.570
0.086
15.9
CONT.
Roots
1.074
—
0.0



Length




Coverage






TP2




TP2





LNU245
Roots
2.500
0.087
12.7
LNU176
Roots
1.326
0.300
23.4



Length




Coverage






TP2




TP2





LNU247
Roots
2.725
0.109
22.9
LNU214
Roots
1.406
0.080
30.9



Length




Coverage






TP2




TP2





LNU251
Roots
2.349
0.343
5.9
LNU223
Roots
1.293
0.141
20.4



Length




Coverage






TP2




TP2





LNU284
Roots
2.335
0.488
5.3
LNU233
Roots
1.428
0.093
32.9



Length




Coverage






TP2




TP2





LNU289
Roots
2.995
0.000
35.1
LNU245
Roots
1.265
0.125
17.8



Length




Coverage






TP2




TP2





LNU70
Roots
2.416
0.313
9.0
LNU247
Roots
1.536
0.005
43.0



Length




Coverage






TP2




TP2





LNU85
Roots
2.854
0.059
28.7
LNU284
Roots
1.377
0.377
28.2



Length




Coverage






TP2




TP2





LNU86
Roots
2.359
0.242
6.4
LNU289
Roots
1.643
0.001
53.0



Length




Coverage






TP2




TP2





CONT.
Roots
3.944
—
0.0
LNU70
Roots
1.242
0.329
15.6



Length




Coverage






TP3




TP2





LNU176
Roots
4.150
0.692
5.2
LNU85
Roots
1.648
0.094
53.4



Length




Coverage






TP3




TP2





LNU214
Roots
4.160
0.286
5.5
LNU86
Roots
1.180
0.402
9.9



Length




Coverage






TP3




TP2





LNU223
Roots
4.286
0.266
8.7
CONT.
Roots
3.404
—
0.0



Length




Coverage






TP3




TP3





LNU233
Roots
4.303
0.439
9.1
LNU176
Roots
3.971
0.527
16.7



Length




Coverage






TP3




TP3





LNU245
Roots
4.127
0.664
4.6
LNU214
Roots
4.249
0.169
24.9



Length




Coverage






TP3




TP3





LNU247
Roots
4.561
0.249
15.7
LNU223
Roots
3.730
0.596
9.6



Length




Coverage






TP3




TP3





LNU251
Roots
3.996
0.778
1.3
LNU233
Roots
4.290
0.194
26.0



Length




Coverage






TP3




TP3





LNU289
Roots
4.422
0.344
12.1
LNU245
Roots
3.652
0.665
7.3



Length




Coverage






TP3




TP3





LNU70
Roots
4.245
0.393
7.6
LNU247
Roots
5.428
0.035
59.5



Length




Coverage






TP3




TP3





LNU85
Roots
4.550
0.343
15.4
LNU284
Roots
4.593
0.294
34.9



Length




Coverage






TP3




TP3





CONT.
Roots
0.787
—
0.0
LNU289
Roots
4.654
0.209
36.7



Length




Coverage






TP1




TP3





LNU105
Roots
0.875
0.213
11.2
LNU70
Roots
3.701
0.708
8.7



Length




Coverage






TP1




TP3





LNU123
Roots
0.837
0.514
6.3
LNU85
Roots
4.066
0.532
19.5



Length




Coverage






TP1




TP3





LNU13
Roots
0.795
0.911
0.9
CONT.
Roots
0.233
—
0.0



Length




Coverage






TP1




TP1





LNU134
Roots
0.871
0.124
10.6
LNU105
Roots
0.261
0.329
12.1



Length




Coverage






TP1




TP1





LNU190
Roots
0.821
0.614
4.3
LNU123
Roots
0.238
0.912
1.9



Length




Coverage






TP1




TP1





LNU198
Roots
0.819
0.606
4.1
LNU134
Roots
0.284
0.151
21.8



Length




Coverage






TP1




TP1





LNU200
Roots
1.049
0.020
33.3
LNU190
Roots
0.262
0.395
12.5



Length




Coverage






TP1




TP1





CONT.
Roots
3.113
—
0.0
LNU198
Roots
0.257
0.466
10.4



Length




Coverage






TP2




TP1





LNU105
Roots
3.270
0.198
5.1
LNU200
Roots
0.348
0.006
49.4



Length




Coverage






TP2




TP1





LNU134
Roots
3.134
0.799
0.7
CONT.
Roots
2.289
—
0.0



Length




Coverage






TP2




TP2





LNU190
Roots
3.121
0.943
0.3
LNU198
Roots
2.307
0.891
0.8



Length




Coverage






TP2




TP2





LNU198
Roots
3.190
0.558
2.5
LNU200
Roots
2.426
0.708
6.0



Length




Coverage






TP2




TP2





LNU200
Roots
3.511
0.295
12.8
CONT.
Roots
0.029
—
0.0



Length




Coverage






TP2




TP1





CONT.
Roots
0.308
—
0.0
LNU243
Roots
0.071
0.210
146.6



Length




Coverage






TP1




TP1





LNU243
Roots
0.507
0.184
64.4
LNU244
Roots
0.049
0.147
70.0



Length




Coverage






TP1




TP1





LNU244
Roots
0.481
0.061
55.9
LNU262
Roots
0.049
0.097
71.8



Length




Coverage






TP1




TP1





LNU262
Roots
0.432
0.048
40.2
LNU29
Roots
0.047
0.117
62.4



Length




Coverage






TP1




TP1





LNU29
Roots
0.406
0.242
31.7
LNU51
Roots
0.095
0.003
228.5



Length




Coverage






TP1




TP1





LNU51
Roots
0.610
0.003
98.0
LNU60
Roots
0.043
0.351
48.4



Length




Coverage






TP1




TP1





LNU60
Roots
0.330
0.681
6.9
CONT.
Roots
1.681
—
0.0



Length




Coverage






TP1




TP2





CONT.
Roots
2.538
—
0.0
LNU225
Roots
1.688
0.972
0.4



Length




Coverage






TP2




TP2





LNU243
Roots
2.561
0.935
0.9
LNU262
Roots
1.827
0.531
8.7



Length




Coverage






TP2




TP2





LNU262
Roots
2.725
0.293
7.4
LNU51
Roots
1.934
0.479
15.1



Length




Coverage






TP2




TP2





LNU29
Roots
2.690
0.470
6.0
LNU60
Roots
2.225
0.191
32.3



Length




Coverage






TP2




TP2





LNU51
Roots
3.044
0.220
19.9
CONT.
Roots
4.355
—
0.0



Length




Coverage






TP2




TP3





LNU60
Roots
2.872
0.149
13.2
LNU225
Roots
5.308
0.168
21.9



Length




Coverage






TP2




TP3





CONT.
Roots_Length_TP3
4.714
—
0.0
LNU243
Roots
4.393
0.935
0.9








Coverage











TP3





LNU51
Roots_Length_TP3
4.998
0.709
6.0
LNU262
Roots
4.744
0.431
8.9








Coverage











TP3





LNU60
Roots_Length_TP3
5.050
0.471
7.1
LNU266
Roots
4.867
0.449
11.8








Coverage











TP3










LNU29
Roots
4.473
0.781
2.7








Coverage











TP3










LNU51
Roots
4.551
0.792
4.5








Coverage











TP3










LNU60
Roots
6.276
0.084
44.1








Coverage











TP3





Table 65.


“CONT.”—Control;


“Ave.”—Average;


“% Incr.” = % increment.






The genes listed in Tables 66, 67, 68 and 69 have improved plant growth rate (growth rate of the leaf area, root coverage and root length) when grown under limiting nitrogen growth conditions, compared to control plants. Plants showing fast growth rate show a better plant establishment in soil under nitrogen deficient conditions. Faster growth was observed when growth rate of leaf area and root length and coverage was measured. The genes were cloned under the regulation of a constitutive promoter (At6669) or root preferred promoter (RootP). The evaluation of each gene was performed by testing the performance of different number of events. Some of the genes were evaluated in more than one tissue culture assay and the results obtained where positive as well. Event with p-value <0.1 was considered statistically significant.







TABLE 66







Genes showing improved plant growth rate at nitrogen deficient conditions (T2 generation)













RGR Of Roots



RGR Of Leaf Area

Coverage
















Gene


p-
%
Gene


p-
%


Name
Event #
Ave.
value
incr.
Name
Event #
Ave.
value
incr.



















CONT.
—
0.055
—
0.0
CONT.
—
0.728
—
0.0


LNU100
14474.3
0.088
0.002
60.1
LNU100
14474.3
1.190
0.000
63.4


LNU100
14473.1
0.065
0.103
18.6
LNU100
14473.1
0.840
0.202
15.4


LNU104
25033.3
0.077
0.004
39.3
LNU100
14471.4
0.837
0.160
15.0


LNU104
25034.1
0.072
0.038
31.5
LNU104
25033.3
0.891
0.075
22.4


LNU213
24654.4
0.096
0.005
73.8
LNU104
25034.1
0.762
0.650
4.7


LNU213
24653.2
0.086
0.000
55.7
LNU213
24653.2
1.117
0.000
53.4


LNU213
24651.1
0.060
0.464
8.2
LNU213
24654.4
1.027
0.051
41.1


LNU218
24783.2
0.076
0.044
37.9
LNU213
24653.1
0.837
0.214
14.9


LNU218
24781.7
0.075
0.070
35.8
LNU213
24651.1
0.760
0.628
4.4


LNU4
25134.1
0.081
0.001
47.6
LNU218
24783.2
1.061
0.012
45.7


LNU4
25134.1
0.065
0.267
17.5
LNU218
24781.7
0.814
0.498
11.9


LNU48
24802.2
0.089
0.001
61.2
LNU4
25134.2
0.965
0.013
32.6


LNU48
24803.2
0.071
0.019
28.6
LNU4
25134.1
0.832
0.252
14.2


LNU48
24804.4
0.070
0.060
27.8
LNU4
25131.1
0.762
0.625
4.7


LNU8
25063.1
0.091
0.000
65.8
LNU48
24802.2
1.237
0.000
69.9


LNU8
25063.6
0.080
0.076
44.7
LNU48
24804.4
1.063
0.001
46.0


LNU94
24833.3
0.074
0.023
34.5
LNU48
24802.1
0.776
0.586
6.6


LNU94
24834.1
0.063
0.392
14.3
LNU48
24803.2
0.771
0.607
5.9


LNU94
24834.4
0.062
0.403
13.4
LNU8
25063.1
1.435
0.000
97.1


CONT.
—
0.081
—
0.0
LNU8
25063.6
0.933
0.267
28.1


LNU1
24681.3
0.104
0.206
28.0
LNU94
24833.3
0.922
0.042
26.6


LNU1
24682.1
0.097
0.321
19.8
LNU94
24834.4
0.805
0.441
10.6


LNU1
24684.1
0.097
0.296
19.3
CONT.
—
0.887
—
0.0


LNU1
24681.1
0.095
0.370
17.0
LNU1
24682.2
1.298
0.113
46.3


LNU1
24682.2
0.092
0.542
12.9
LNU1
24684.1
1.291
0.000
45.5


LNU133
24744.3
0.114
0.079
40.6
LNU1
24681.1
1.248
0.019
40.6


LNU133
24741.1
0.105
0.096
29.6
LNU1
24681.3
1.092
0.095
23.1


LNU133
24744.2
0.087
0.712
6.9
LNU1
24682.1
0.989
0.258
11.5


LNU175
24732.4
0.129
0.022
59.1
LNU133
24744.3
1.638
0.001
84.6


LNU175
24732.1
0.124
0.007
53.3
LNU133
24741.1
1.461
0.000
64.7


LNU175
24734.4
0.095
0.350
17.4
LNU133
24741.2
1.071
0.021
20.7


LNU175
24731.2
0.087
0.712
7.0
LNU133
24744.2
1.049
0.150
18.3


LNU178
14614.5
0.115
0.078
41.9
LNU133
24742.2
1.020
0.263
14.9


LNU178
14611.5
0.106
0.129
30.8
LNU175
24732.4
1.929
0.002
117.4


LNU178
14612.1
0.089
0.587
9.5
LNU175
24732.1
1.756
0.000
97.9


LNU178
14611.1
0.085
0.782
5.0
LNU175
24734.4
1.182
0.014
33.3


LNU215
24664.3
0.113
0.068
39.1
LNU175
24731.2
1.070
0.115
20.6


LNU215
24661.4
0.087
0.711
6.9
LNU175
24733.4
1.019
0.271
14.9


LNU24
24973.1
0.104
0.160
28.2
LNU178
14611.5
1.630
0.000
83.7


LNU24
24971.2
0.101
0.203
24.2
LNU178
14611.1
1.245
0.035
40.3


LNU24
24971.4
0.100
0.374
23.4
LNU178
14614.5
1.219
0.066
37.4


LNU6
24992.3
0.106
0.084
31.0
LNU178
14612.1
1.002
0.265
13.0


LNU6
24994.1
0.092
0.476
13.4
LNU178
14611.4
0.985
0.237
11.0


LNU6
24993.3
0.089
0.601
10.2
LNU215
24664.3
1.409
0.003
58.8


LNU6
24994.2
0.088
0.658
8.3
LNU215
24661.4
1.132
0.043
27.6


LNU82
24823.1
0.095
0.328
17.5
LNU215
24664.2
1.100
0.015
24.0


LNU82
24824.1
0.086
0.758
6.3
LNU215
24663.4
1.034
0.121
16.5


LNU9
25003.1
0.093
0.468
14.8
LNU24
24973.1
1.501
0.000
69.2


LNU9
25001.3
0.089
0.598
10.1
LNU24
24971.4
1.350
0.001
52.2


CONT.
—
0.058
—
0.0
LNU24
24971.2
1.271
0.001
43.3


LNU120
25463.7
0.081
0.001
39.9
LNU24
24971.3
0.965
0.419
8.8


LNU120
25463.3
0.074
0.019
27.4
LNU24
24972.1
0.952
0.578
7.3


LNU124
14501.7
0.079
0.007
36.5
LNU6
24992.3
1.524
0.001
71.8


LNU124
14502.7
0.068
0.125
17.3
LNU6
24994.2
1.176
0.024
32.5


LNU124
14501.1
0.065
0.277
12.6
LNU6
24994.5
1.067
0.264
20.3


LNU132
14102.9
0.066
0.251
14.0
LNU6
24993.3
1.045
0.147
17.8


LNU132
14102.7
0.064
0.379
10.6
LNU6
24994.1
1.012
0.332
14.1


LNU140
14112.7
0.097
0.000
67.6
LNU82
24823.1
1.167
0.036
31.5


LNU140
14111.6
0.070
0.081
21.0
LNU82
24824.2
1.126
0.036
27.0


LNU140
14114.8
0.063
0.435
8.5
LNU82
24824.3
0.979
0.370
10.4


LNU140
14112.6
0.060
0.732
3.8
LNU82
24824.1
0.978
0.555
10.2


LNU180
24724.3
0.099
0.000
71.3
LNU9
25001.1
1.294
0.001
45.8


LNU180
24723.3
0.080
0.009
37.5
LNU9
25003.1
1.232
0.023
38.9


LNU196
25534.1
0.069
0.171
18.6
LNU9
25001.2
1.118
0.135
26.0


LNU196
25533.1
0.066
0.349
14.2
LNU9
25001.3
1.117
0.041
25.9


LNU20
24932.4
0.068
0.174
17.3
CONT.
—
0.908
—
0.0


LNU20
24933.2
0.065
0.390
12.7
LNU120
25463.7
1.427
0.000
57.3


LNU36
25562.3
0.085
0.024
46.0
LNU120
25463.3
1.010
0.346
11.3


LNU71
25853.4
0.087
0.001
50.3
LNU124
14502.1
1.162
0.095
28.1


LNU71
25852.4
0.060
0.773
3.2
LNU124
14501.7
1.135
0.152
25.0


CONT.
—
0.060
—
0.0
LNU124
14502.7
1.003
0.415
10.6


LNU1
24681.3
0.073
0.306
20.5
LNU124
14501.1
0.960
0.683
5.8


LNU110
24952.3
0.090
0.013
48.7
LNU132
14102.7
1.130
0.095
24.5


LNU110
24953.3
0.079
0.110
30.2
LNU132
14102.9
1.129
0.060
24.4


LNU110
24953.2
0.069
0.323
14.0
LNU140
14112.7
1.571
0.000
73.0


LNU175
24732.2
0.087
0.008
44.2
LNU140
14111.6
1.145
0.048
26.2


LNU175
24733.4
0.070
0.218
16.4
LNU140
14112.6
0.970
0.554
6.9


LNU19
25151.1
0.090
0.001
48.9
LNU140
14114.8
0.966
0.603
6.5


LNU19
25153.3
0.079
0.063
31.5
LNU180
24724.3
1.590
0.000
75.2


LNU215
24664.2
0.091
0.000
50.8
LNU180
24723.3
1.265
0.004
39.4


LNU215
24663.4
0.078
0.016
28.4
LNU180
24721.4
0.942
0.765
3.8


LNU215
24663.3
0.071
0.182
17.5
LNU20
24932.4
1.177
0.028
29.7


LNU215
24661.4
0.065
0.558
7.1
LNU20
24933.2
1.021
0.453
12.5


LNU27
24873.1
0.088
0.012
45.4
LNU36
25562.3
1.375
0.017
51.5


LNU27
24873.4
0.080
0.048
32.1
LNU36
25562.4
1.090
0.208
20.1


LNU27
24872.4
0.067
0.357
11.3
LNU71
25853.4
1.170
0.066
29.0


LNU44
24924.3
0.112
0.001
84.9
CONT.
—
0.869
—
0.0


LNU44
24924.2
0.089
0.004
48.1
LNU1
24682.1
0.948
0.453
9.2


LNU44
24922.3
0.076
0.072
25.2
LNU110
24952.3
1.151
0.199
32.5


LNU54
24903.5
0.102
0.000
69.7
LNU110
24953.2
0.948
0.500
9.1


LNU54
24901.2
0.097
0.005
60.2
LNU175
24732.2
1.291
0.013
48.7


LNU54
24903.3
0.078
0.058
29.8
LNU175
24732.1
1.018
0.248
17.2


LNU79
24884.4
0.102
0.000
68.5
LNU175
24734.4
0.927
0.633
6.7


LNU79
24881.1
0.088
0.003
46.2
LNU19
25151.1
1.046
0.194
20.4


LNU79
24884.3
0.077
0.045
27.7
LNU215
24664.2
1.315
0.001
51.4


LNU79
24882.2
0.068
0.280
13.4
LNU215
24663.4
1.179
0.010
35.8


CONT.
—
0.078
—
0.0
LNU215
24663.3
1.033
0.208
19.0


LNU109
24891.5
0.105
0.016
35.4
LNU215
24661.4
1.030
0.127
18.6


LNU109
24892.5
0.097
0.142
24.5
LNU215
24663.1
0.907
0.688
4.5


LNU109
24892.6
0.095
0.219
21.5
LNU27
24873.1
1.325
0.016
52.6


LNU109
24891.2
0.088
0.462
12.6
LNU27
24873.4
1.072
0.138
23.5


LNU110
24952.1
0.155
0.000
99.4
LNU44
24924.2
1.395
0.002
60.7


LNU110
24952.3
0.111
0.031
42.2
LNU44
24924.3
1.315
0.018
51.4


LNU110
24953.2
0.106
0.030
36.6
LNU44
24922.3
1.296
0.044
49.2


LNU133
24744.3
0.121
0.000
55.2
LNU44
24923.3
1.123
0.025
29.3


LNU133
24741.1
0.103
0.017
32.5
LNU54
24903.5
1.573
0.000
81.2


LNU133
24741.2
0.097
0.033
25.0
LNU54
24901.2
1.405
0.015
61.7


LNU133
24744.2
0.088
0.329
13.5
LNU54
24903.3
1.367
0.008
57.3


LNU133
24742.2
0.083
0.581
6.3
LNU54
24902.4
1.137
0.031
30.9


LNU19
25151.1
0.106
0.007
36.3
LNU79
24881.1
1.407
0.001
62.0


LNU27
24873.4
0.125
0.000
60.1
LNU79
24884.4
1.407
0.000
61.9


LNU44
24922.3
0.113
0.000
44.6
LNU79
24884.3
1.127
0.095
29.8


LNU44
24924.3
0.091
0.308
17.0
LNU79
24882.2
1.053
0.073
21.2


LNU44
24923.1
0.085
0.518
8.7
LNU79
24883.2
0.899
0.782
3.5


LNU54
24901.2
0.119
0.000
52.2
CONT.
—
1.054
—
0.0


LNU54
24903.5
0.104
0.031
33.2
LNU109
24891.5
1.313
0.018
24.6


LNU54
24902.4
0.094
0.056
20.3
LNU109
24892.6
1.256
0.186
19.1


LNU6
24994.5
0.112
0.005
43.2
LNU110
24952.1
2.265
0.000
114.9


LNU6
24992.3
0.107
0.012
36.7
LNU110
24952.3
1.456
0.012
38.1


LNU79
24884.4
0.120
0.000
54.1
LNU110
24953.2
1.221
0.308
15.8


LNU79
24881.1
0.107
0.012
36.9
LNU110
24954.3
1.163
0.333
10.4


LNU79
24882.2
0.098
0.056
26.4
LNU133
24744.3
1.717
0.000
62.9


LNU79
24884.3
0.092
0.228
18.2
LNU133
24741.1
1.470
0.026
39.5


CONT.
—
0.052
—
0.0
LNU133
24742.2
1.203
0.166
14.2


LNU109
24892.8
0.104
0.000
101.5
LNU133
24741.2
1.123
0.527
6.5


LNU109
24891.2
0.104
0.002
100.4
LNU19
25151.1
1.490
0.008
41.3


LNU109
24891.5
0.072
0.136
38.7
LNU27
24873.4
1.673
0.000
58.7


LNU109
24892.5
0.060
0.394
15.2
LNU44
24922.3
1.944
0.000
84.4


LNU143
25975.3
0.081
0.021
56.0
LNU54
24901.2
1.669
0.000
58.4


LNU143
25972.1
0.074
0.032
42.5
LNU54
24903.5
1.243
0.185
18.0


LNU143
25975.2
0.069
0.088
34.3
LNU6
24992.3
1.678
0.001
59.2


LNU143
25971.5
0.066
0.085
26.9
LNU6
24994.5
1.323
0.144
25.5


LNU143
25971.2
0.060
0.346
16.5
LNU6
24994.2
1.157
0.317
9.7


LNU154
14604.7
0.092
0.002
78.6
LNU79
24884.4
1.756
0.000
66.6


LNU154
14604.6
0.086
0.006
66.7
LNU79
24881.1
1.355
0.008
28.6


LNU154
14601.6
0.077
0.098
48.3
LNU79
24882.2
1.263
0.206
19.8


LNU154
14602.8
0.069
0.185
34.0
LNU79
24884.3
1.223
0.225
16.1


LNU154
14604.4
0.069
0.082
33.4
LNU79
24883.2
1.101
0.613
4.5


LNU196
25532.2
0.113
0.000
117.6
CONT.
—
0.737
—
0.0


LNU196
25534.1
0.089
0.017
71.3
LNU109
24892.8
1.439
0.000
95.2


LNU196
25531.2
0.073
0.034
40.4
LNU109
24891.2
1.093
0.066
48.3


LNU196
25532.1
0.065
0.357
25.8
LNU109
24891.5
1.071
0.028
45.2


LNU207
24642.5
0.094
0.001
82.1
LNU143
25975.3
1.056
0.012
43.2


LNU207
24642.4
0.094
0.001
81.0
LNU143
25972.1
0.980
0.035
32.9


LNU207
24644.18
0.080
0.006
54.8
LNU143
25975.2
0.917
0.233
24.3


LNU207
24641.1
0.056
0.655
7.3
LNU143
25971.5
0.877
0.338
19.0


LNU207
24644.13
0.055
0.786
5.7
LNU154
14604.6
1.099
0.009
49.0


LNU288
14562.12
0.075
0.048
44.1
LNU154
14601.6
1.081
0.079
46.6


LNU288
14562.7
0.075
0.022
44.1
LNU154
14604.7
1.015
0.024
37.7


LNU288
14562.1
0.069
0.154
32.5
LNU154
14602.8
0.982
0.253
33.2


LNU288
14564.9
0.066
0.089
27.6
LNU196
25532.2
1.650
0.000
123.9


LNU288
14562.9
0.064
0.261
23.7
LNU196
25534.1
1.327
0.052
80.0


LNU50
26024.2
0.085
0.007
64.1
LNU196
25532.1
0.971
0.248
31.7


LNU50
26023.2
0.073
0.028
40.6
LNU196
25531.2
0.817
0.525
10.8


LNU50
26025.4
0.065
0.125
26.0
LNU196
25533.1
0.783
0.607
6.2


LNU50
26022.1
0.062
0.321
19.8
LNU207
24642.4
1.393
0.010
89.0


LNU50
26023.5
0.056
0.558
9.1
LNU207
24642.5
1.164
0.002
57.9


LNU52
25723.2
0.126
0.000
143.1
LNU207
24644.18
1.102
0.010
49.5


LNU52
25721.4
0.094
0.034
81.1
LNU207
24644.13
0.920
0.279
24.8


LNU52
25721.3
0.086
0.008
66.2
LNU288
14562.1
1.018
0.124
38.1


CONT.
—
0.064
—
0.0
LNU288
14562.7
0.987
0.056
33.9


LNU143
25975.2
0.075
0.257
15.9
LNU288
14564.9
0.939
0.037
27.3


LNU154
14602.8
0.079
0.120
22.7
LNU288
14562.12
0.823
0.473
11.7


LNU154
14604.4
0.070
0.618
8.3
LNU288
14562.9
0.823
0.387
11.7


LNU207
24642.5
0.092
0.008
43.2
LNU50
26023.2
1.105
0.011
49.9


LNU207
24641.1
0.079
0.154
22.6
LNU50
26024.2
1.085
0.063
47.2


LNU207
24642.4
0.069
0.560
7.5
LNU50
26025.4
0.978
0.038
32.6


LNU211
24774.4
0.271
0.173
321.2
LNU50
26023.5
0.855
0.264
16.0


LNU52
25721.1
0.091
0.013
42.2
LNU50
26022.1
0.810
0.542
9.9


LNU52
25723.2
0.085
0.074
31.7
LNU52
25723.2
1.741
0.000
136.1


LNU52
25723.1
0.081
0.191
25.5
LNU52
25721.4
1.195
0.045
62.1


LNU52
25721.2
0.073
0.369
13.7
LNU52
25721.3
1.193
0.024
61.8


LNU69
14571.1
0.081
0.108
26.1
LNU52
25723.1
0.805
0.623
9.2


LNU69
14572.9
0.079
0.197
23.1
CONT.
—
1.006
—
0.0


LNU69
14572.8
0.074
0.362
14.4
LNU143
25975.2
1.194
0.208
18.7


CONT.
—
0.072
—
0.0
LNU143
25975.3
1.085
0.620
7.8


LNU150
24842.9
0.115
0.006
60.7
LNU154
14602.8
1.225
0.116
21.7


LNU150
24841.9
0.082
0.477
15.1
LNU207
24642.5
1.333
0.028
32.4


LNU179
24632.5
0.097
0.103
35.0
LNU207
24641.1
1.265
0.082
25.7


LNU179
24631.9
0.084
0.441
16.8
LNU207
24642.4
1.159
0.228
15.2


LNU232
26003.7
0.083
0.456
15.9
LNU211
24771.1
1.136
0.330
12.9


LNU235
26184.4
0.101
0.063
40.9
LNU52
25721.1
1.458
0.019
44.8


LNU235
26185.3
0.100
0.080
40.3
LNU52
25723.1
1.362
0.084
35.4


LNU242
25473.1
0.096
0.109
33.9
LNU52
25721.2
1.328
0.093
32.0


LNU242
25474.1
0.079
0.639
9.9
LNU52
25723.2
1.208
0.271
20.1


LNU242
25471.1
0.079
0.638
9.8
LNU69
14571.1
1.398
0.027
38.9


LNU76
26423.1
0.097
0.093
35.9
LNU69
14572.9
1.140
0.342
13.2


LNU76
26421.2
0.089
0.241
24.9
LNU69
14572.8
1.105
0.378
9.8


LNU76
26425.1
0.086
0.377
19.5
CONT.
—
0.860
—
0.0


LNU76
26421.1
0.077
0.746
7.3
LNU150
24842.9
1.560
0.001
81.4


LNU95
13985.11
0.101
0.055
40.8
LNU150
24841.9
1.169
0.141
36.0


LNU95
13985.15
0.079
0.623
10.3
LNU150
24843.5
1.141
0.199
32.7


LNU95
13985.12
0.076
0.784
5.9
LNU179
24632.5
1.501
0.005
74.5


CONT.
—
0.067
—
0.0
LNU179
24631.9
1.269
0.062
47.5


LNU118
14013.6
0.078
0.267
16.4
LNU179
24631.6
0.933
0.723
8.5


LNU118
14012.15
0.076
0.371
12.9
LNU232
26003.7
1.110
0.227
29.1


LNU150
24841.6
0.077
0.375
13.8
LNU235
26184.4
1.576
0.001
83.3


LNU150
24841.9
0.075
0.470
11.0
LNU235
26185.3
1.416
0.013
64.7


LNU150
24842.9
0.072
0.666
7.1
LNU235
26182.1
1.048
0.361
21.8


LNU179
24631.6
0.079
0.251
17.0
LNU235
26184.2
1.019
0.431
18.5


LNU179
24631.7
0.078
0.279
15.7
LNU242
25473.1
1.482
0.004
72.3


LNU179
24632.7
0.072
0.601
7.2
LNU242
25474.1
1.275
0.056
48.3


LNU232
26001.5
0.082
0.155
21.2
LNU76
26425.1
1.342
0.026
56.1


LNU232
26003.3
0.070
0.741
4.7
LNU76
26421.2
1.194
0.112
38.8


LNU235
26184.4
0.115
0.000
71.6
LNU76
26423.1
1.177
0.128
36.9


LNU235
26185.2
0.113
0.000
68.3
LNU76
26421.1
1.021
0.471
18.7


LNU235
26184.2
0.094
0.007
39.9
LNU95
13985.11
1.522
0.003
76.9


LNU235
26182.1
0.071
0.718
5.1
LNU95
13985.15
1.128
0.194
31.2


LNU242
25474.1
0.098
0.004
45.3
LNU95
13985.19
1.100
0.280
27.9


LNU288
14563.9
0.096
0.006
43.3
LNU95
13985.12
1.090
0.273
26.8


LNU288
14562.1
0.090
0.024
33.5
LNU95
13985.16
0.947
0.670
10.1


LNU288
14562.7
0.081
0.183
20.1
CONT.
—
1.017
—
0.0


LNU288
14564.9
0.080
0.203
18.5
LNU232
26001.5
1.259
0.085
23.7


LNU76
26421.2
0.090
0.068
33.6
LNU235
26184.4
1.645
0.000
61.7


LNU76
26422.2
0.086
0.063
28.3
LNU235
26185.2
1.636
0.000
60.8


LNU76
26423.1
0.086
0.099
27.9
LNU235
26184.2
1.205
0.181
18.4


LNU95
13985.16
0.126
0.000
87.0
LNU242
25474.1
1.210
0.179
19.0


LNU95
13985.15
0.113
0.000
68.6
LNU288
14563.9
1.512
0.002
48.6


LNU95
13985.12
0.092
0.013
36.2
LNU288
14562.1
1.225
0.102
20.4


CONT.
—
0.066
—
0.0
LNU288
14564.9
1.119
0.472
10.0


LNU101
27632.7
0.080
0.040
21.0
LNU288
14562.7
1.065
0.698
4.7


LNU128
26515.3
0.087
0.003
30.2
LNU76
26421.2
1.389
0.037
36.6


LNU192
28315.2
0.085
0.007
27.6
LNU76
26423.1
1.131
0.435
11.1


LNU192
28313.2
0.070
0.605
5.1
LNU76
26422.2
1.123
0.483
10.4


LNU206
27621.2
0.077
0.140
15.4
LNU95
13985.16
1.594
0.000
56.7


LNU211
24771.1
0.073
0.298
10.1
LNU95
13985.12
1.420
0.014
39.6


LNU211
24773.2
0.071
0.480
7.1
LNU95
13985.15
1.417
0.004
39.3


LNU282
27563.3
0.082
0.030
22.7
CONT.
—
0.870
—
0.0


LNU282
27563.1
0.078
0.103
17.4
LNU101
27632.7
1.210
0.000
39.0


LNU282
27562.1
0.075
0.286
12.5
LNU101
27632.1
0.985
0.153
13.2


LNU69
14571.1
0.089
0.001
33.3
LNU101
27635.1
0.976
0.280
12.1


LNU75
27572.2
0.072
0.395
8.8
LNU128
26515.3
1.467
0.000
68.5


LNU75
27572.1
0.072
0.379
8.5
LNU128
26511.5
1.191
0.000
36.8


CONT.
—
0.056
—
0.0
LNU128
26515.2
1.071
0.024
23.0


LNU118
14012.15
0.059
0.772
3.7
LNU128
26511.4
0.891
0.790
2.4


LNU206
27621.2
0.097
0.018
72.5
LNU192
28315.2
1.393
0.000
60.0


LNU206
27621.1
0.085
0.007
50.7
LNU192
28313.2
1.211
0.000
39.2


LNU206
27622.1
0.077
0.021
37.3
LNU192
28313.3
1.079
0.026
23.9


LNU206
27622.4
0.073
0.068
29.9
LNU192
28312.2
0.927
0.454
6.5


LNU249
26153.1
0.084
0.003
48.8
LNU206
27621.2
1.252
0.000
43.9


LNU249
26154.2
0.071
0.081
26.7
LNU206
27621.1
1.059
0.023
21.6


LNU249
26152.4
0.060
0.659
6.4
LNU206
27622.1
0.985
0.140
13.2


LNU282
27563.1
0.076
0.016
34.7
LNU211
24771.1
1.374
0.000
57.8


LNU282
27565.2
0.075
0.051
33.1
LNU211
24773.2
1.127
0.006
29.5


LNU288
14564.8
0.087
0.001
55.1
LNU282
27562.1
1.477
0.000
69.7


LNU288
14563.9
0.082
0.000
46.0
LNU282
27563.3
1.333
0.000
53.2


LNU288
14562.9
0.070
0.066
25.0
LNU282
27563.1
1.262
0.000
45.0


LNU288
14563.6
0.065
0.305
14.8
LNU282
27565.2
0.966
0.204
11.0


LNU288
14562.7
0.060
0.678
5.9
LNU69
14571.1
1.528
0.000
75.6


LNU75
27572.3
0.094
0.000
67.3
LNU69
14573.5
1.115
0.004
28.2


LNU75
27572.2
0.081
0.019
43.6
LNU69
14572.9
0.895
0.798
2.9


LNU75
27571.2
0.081
0.004
43.3
LNU75
27572.2
1.373
0.000
57.8


LNU75
27571.4
0.079
0.005
40.0
LNU75
27572.1
1.152
0.001
32.4


CONT.
—
0.076
—
0.0
LNU75
27572.3
1.006
0.148
15.6


LNU112
28212.4
0.082
0.565
7.8
LNU75
27571.4
0.957
0.334
9.9


LNU14
27823.2
0.083
0.490
9.5
CONT.
—
0.804
—
0.0


LNU183
24863.1
0.098
0.030
29.7
LNU101
27632.5
0.982
0.112
22.2


LNU183
24863.12
0.091
0.130
20.4
LNU118
14012.15
1.037
0.038
29.1


LNU183
24864.6
0.085
0.487
12.6
LNU118
14012.12
0.843
0.692
4.9


LNU183
24865.1
0.080
0.652
6.2
LNU206
27621.2
1.502
0.000
86.9


LNU201
28223.1
0.084
0.410
11.6
LNU206
27622.4
1.352
0.000
68.3


LNU201
28222.2
0.078
0.792
3.6
LNU206
27622.1
1.218
0.004
51.5


LNU268
26044.2
0.083
0.520
9.7
LNU206
27621.1
1.124
0.018
39.9


CONT.
—
0.066
—
0.0
LNU249
26153.1
1.209
0.007
50.5


LNU11
28205.1
0.089
0.021
34.1
LNU249
26154.2
1.142
0.032
42.0


LNU11
28205.2
0.075
0.357
12.2
LNU249
26152.4
1.042
0.075
29.6


LNU11
28204.1
0.074
0.414
11.5
LNU249
26151.1
0.841
0.753
4.7


LNU11
28203.2
0.072
0.539
7.7
LNU282
27563.1
1.273
0.001
58.4


LNU112
28212.4
0.081
0.105
22.1
LNU282
27565.2
1.273
0.002
58.4


LNU112
28212.1
0.078
0.181
17.6
LNU282
27562.1
0.836
0.765
4.0


LNU112
28212.3
0.076
0.356
14.5
LNU288
14563.9
1.420
0.000
76.7


LNU14
27821.3
0.091
0.009
36.3
LNU288
14563.6
1.166
0.018
45.1


LNU14
27821.4
0.080
0.139
21.0
LNU288
14564.8
1.158
0.007
44.1


LNU14
27824.2
0.073
0.440
10.6
LNU288
14562.9
1.104
0.004
37.3


LNU183
24864.6
0.108
0.000
63.2
LNU288
14562.7
1.029
0.064
28.1


LNU183
24863.12
0.099
0.001
49.5
LNU75
27572.2
1.648
0.000
105.0


LNU183
24865.1
0.095
0.009
42.5
LNU75
27571.2
1.413
0.000
75.8


LNU183
24863.1
0.089
0.012
34.3
LNU75
27572.3
1.342
0.000
67.0


LNU191
28325.4
0.081
0.112
21.4
LNU75
27571.4
1.151
0.007
43.2


LNU191
28324.2
0.078
0.228
17.5
CONT.
—
1.337
—
0.0


LNU191
28321.3
0.073
0.438
10.2
LNU112
28212.4
1.471
0.446
10.0


LNU191
28323.1
0.073
0.468
9.9
LNU183
24865.1
1.454
0.553
8.7


LNU201
28222.2
0.097
0.004
46.8
LNU183
24863.1
1.423
0.621
6.5


LNU201
28223.3
0.083
0.110
25.1
LNU201
28222.2
1.426
0.669
6.7


LNU201
28221.3
0.075
0.365
12.4
LNU268
26044.2
1.586
0.220
18.6


LNU268
26043.4
0.077
0.218
16.2
CONT.
—
1.041
—
0.0


LNU268
26041.4
0.075
0.332
13.4
LNU11
28205.2
1.468
0.006
41.0


CONT.
—
0.051
—
0.0
LNU11
28203.2
1.369
0.063
31.5


LNU107
14583.8
0.076
0.007
48.4
LNU11
28202.5
1.338
0.069
28.6


LNU107
14585.5
0.065
0.100
26.5
LNU11
28205.1
1.338
0.045
28.5


LNU107
14584.9
0.057
0.561
11.1
LNU11
28204.1
1.126
0.564
8.1


LNU116
14494.5
0.085
0.001
65.8
LNU11
28204.3
1.091
0.747
4.8


LNU116
14492.9
0.080
0.008
55.0
LNU112
28212.1
1.350
0.044
29.7


LNU116
14492.5
0.067
0.072
29.7
LNU112
28212.4
1.142
0.464
9.7


LNU116
14493.6
0.057
0.537
10.1
LNU14
27821.3
1.766
0.000
69.6


LNU121
27713.4
0.092
0.000
78.5
LNU14
27821.4
1.364
0.053
31.0


LNU121
27711.1
0.085
0.001
65.3
LNU14
27824.2
1.190
0.288
14.3


LNU121
25642.2
0.084
0.002
62.9
LNU14
27821.1
1.095
0.671
5.2


LNU121
27713.1
0.072
0.042
40.8
LNU183
24864.6
2.078
0.000
99.6


LNU126
25345.1
0.074
0.016
43.6
LNU183
24863.12
1.703
0.000
63.6


LNU126
25343.1
0.068
0.132
32.1
LNU183
24865.1
1.690
0.000
62.4


LNU126
25343.3
0.063
0.254
21.7
LNU183
24863.1
1.371
0.041
31.7


LNU126
25343.4
0.054
0.749
5.2
LNU191
28325.4
1.453
0.029
39.5


LNU158
27433.3
0.077
0.009
49.2
LNU191
28323.1
1.404
0.028
34.9


LNU158
27433.2
0.075
0.023
45.8
LNU191
28321.3
1.246
0.174
19.7


LNU158
27432.5
0.065
0.225
25.7
LNU191
28324.2
1.114
0.606
7.0


LNU158
27434.1
0.054
0.759
5.3
LNU201
28222.2
1.662
0.000
59.6


LNU177
24762.6
0.092
0.000
79.8
LNU201
28223.3
1.505
0.008
44.6


LNU177
24764.9
0.063
0.216
22.4
LNU201
28221.3
1.212
0.212
16.4


LNU177
24765.2
0.060
0.389
16.0
LNU201
28223.1
1.150
0.497
10.5


LNU182
25384.1
0.083
0.002
61.9
LNU268
26041.4
1.323
0.039
27.0


LNU182
27521.4
0.069
0.028
34.9
LNU268
26041.6
1.273
0.137
22.3


LNU182
25384.5
0.065
0.128
26.1
LNU268
26043.4
1.270
0.108
22.0


LNU182
25384.2
0.057
0.590
11.4
CONT.
—
1.034
—
0.0


LNU2
27842.1
0.062
0.219
20.8
LNU107
14583.8
1.442
0.035
39.5


LNU2
27845.2
0.059
0.329
15.1
LNU107
14585.5
1.213
0.242
17.3


LNU2
27842.3
0.057
0.510
10.6
LNU107
14584.9
1.101
0.695
6.5


LNU225
25991.5
0.062
0.329
20.6
LNU116
14492.9
1.463
0.032
41.6


LNU225
25991.2
0.057
0.617
11.8
LNU116
14494.5
1.394
0.053
34.8


LNU239
26283.2
0.057
0.544
11.4
LNU116
14492.5
1.305
0.120
26.2


LNU57
27854.5
0.059
0.385
14.1
LNU121
27713.4
1.552
0.003
50.1


LNU57
27852.1
0.054
0.717
5.9
LNU121
25642.2
1.486
0.007
43.8


LNU83
27684.1
0.076
0.011
47.3
LNU121
27711.1
1.463
0.009
41.5


LNU83
27685.1
0.055
0.672
7.2
LNU121
27713.1
1.117
0.615
8.0


LNU83
27685.2
0.055
0.693
6.7
LNU126
25345.1
1.417
0.058
37.1


CONT.
—
0.039
—
0.0
LNU126
25343.1
1.367
0.043
32.3


LNU107
14584.9
0.060
0.086
53.1
LNU126
25343.3
1.164
0.485
12.6


LNU107
14585.2
0.051
0.216
30.4
LNU158
27432.5
1.551
0.020
50.0


LNU107
14583.8
0.049
0.326
23.7
LNU158
27433.3
1.537
0.002
48.7


LNU116
14494.5
0.071
0.002
80.2
LNU158
27433.2
1.367
0.047
32.3


LNU116
14492.5
0.068
0.008
74.3
LNU177
24762.6
1.619
0.000
56.6


LNU116
14492.9
0.056
0.084
43.8
LNU177
24764.9
1.270
0.280
22.8


LNU116
14493.6
0.056
0.136
42.3
LNU177
24765.2
1.115
0.596
7.9


LNU116
14491.5
0.049
0.340
24.1
LNU182
25384.1
1.674
0.000
61.9


LNU121
27713.1
0.082
0.002
108.5
LNU182
27521.4
1.443
0.020
39.6


LNU121
27711.1
0.061
0.060
55.3
LNU182
25384.2
1.184
0.409
14.5


LNU121
27713.4
0.056
0.194
42.8
LNU2
27842.1
1.272
0.118
23.0


LNU121
25642.2
0.053
0.123
36.0
LNU2
27842.3
1.202
0.253
16.3


LNU126
25343.3
0.055
0.137
39.2
LNU225
25991.5
1.957
0.000
89.3


LNU126
25343.1
0.055
0.193
39.0
LNU225
25991.2
1.568
0.068
51.7


LNU126
25341.1
0.047
0.375
19.0
LNU239
26284.1
1.173
0.351
13.4


LNU158
27433.3
0.090
0.000
129.9
LNU57
27852.1
1.108
0.646
7.1


LNU158
27432.5
0.071
0.013
80.3
LNU83
27681.4
1.299
0.154
25.7


LNU158
27433.2
0.062
0.028
56.8
LNU83
27684.1
1.142
0.536
10.5


LNU177
24764.12
0.052
0.297
31.3
CONT.
—
1.042
—
0.0


LNU177
24763.6
0.045
0.587
13.9
LNU107
14584.9
1.679
0.000
61.1


LNU177
24762.6
0.044
0.637
11.6
LNU107
14583.8
1.266
0.094
21.5


LNU182
25384.6
0.062
0.027
58.1
LNU107
14585.2
1.110
0.538
6.5


LNU182
25384.2
0.055
0.062
39.4
LNU116
14492.5
1.582
0.011
51.8


LNU2
27842.1
0.065
0.020
64.9
LNU116
14494.5
1.474
0.038
41.4


LNU2
27842.3
0.052
0.204
33.2
LNU116
14493.6
1.214
0.133
16.5


LNU2
27845.2
0.043
0.792
9.5
LNU121
25642.2
1.548
0.003
48.6


LNU225
25991.2
0.067
0.005
70.3
LNU121
27711.1
1.483
0.008
42.3


LNU225
25991.3
0.064
0.033
62.1
LNU121
27713.1
1.469
0.024
41.0


LNU225
25991.8
0.062
0.006
58.4
LNU121
27713.4
1.299
0.148
24.6


LNU225
25991.1
0.044
0.590
12.8
LNU126
25343.1
1.533
0.005
47.1


LNU239
26283.2
0.046
0.481
17.6
LNU126
25343.4
1.138
0.399
9.2


LNU239
26284.2
0.045
0.606
14.6
LNU126
25343.3
1.135
0.547
9.0


LNU239
26284.1
0.045
0.517
14.4
LNU158
27433.3
1.718
0.000
64.9


LNU239
26281.1
0.044
0.624
12.7
LNU158
27432.5
1.552
0.001
48.9


LNU57
27854.5
0.054
0.171
38.4
LNU158
27433.2
1.223
0.182
17.4


LNU57
27852.1
0.045
0.560
13.4
LNU177
24764.12
1.480
0.002
42.0


LNU83
27685.1
0.082
0.001
108.8
LNU177
24763.6
1.139
0.446
9.3


LNU83
27685.2
0.055
0.217
39.2
LNU177
24762.6
1.106
0.569
6.1


LNU83
27681.4
0.053
0.102
34.0
LNU182
25384.1
1.145
0.434
9.8







LNU182
25384.6
1.137
0.510
9.1







LNU182
25384.5
1.120
0.583
7.5







LNU2
27842.1
1.510
0.005
44.9







LNU2
27842.3
1.235
0.123
18.5







LNU2
25713.1
1.173
0.395
12.5







LNU225
25991.3
1.802
0.000
72.9







LNU225
25991.2
1.638
0.000
57.2







LNU225
25991.8
1.355
0.035
30.0







LNU225
25991.1
1.290
0.106
23.8







LNU239
26284.2
1.385
0.010
32.9







LNU57
27852.1
1.618
0.000
55.3







LNU57
27851.2
1.559
0.001
49.6







LNU57
27854.5
1.285
0.052
23.3







LNU57
27854.3
1.105
0.534
6.1







LNU83
27685.1
1.715
0.000
64.6







LNU83
27685.2
1.417
0.007
36.0







LNU83
27681.4
1.399
0.006
34.2





Table 66.


“CONT.”—Control;


“Ave.”—Average;


“% Incr.” = % increment.













TABLE 67







Genes showing improved plant growth rate at nitrogen


deficient conditions (T2 generation)









RGR Of Roots Length













Gene Name
Event #
Average
p-value
% increment

















CONTROL
—
0.508
—
0.0



LNU100
14471.4
0.596
0.019
17.4



LNU100
14474.3
0.592
0.005
16.6



LNU100
14473.1
0.577
0.062
13.6



LNU100
14474.4
0.545
0.162
7.3



LNU104
25034.1
0.610
0.280
20.2



LNU104
25033.3
0.554
0.201
9.1



LNU213
24653.2
0.581
0.019
14.5



LNU213
24652.4
0.535
0.389
5.3



LNU213
24654.4
0.531
0.578
4.6



LNU213
24653.1
0.522
0.713
2.8



LNU218
24783.2
0.579
0.142
14.1



LNU4
25134.2
0.594
0.003
16.9



LNU4
25131.1
0.531
0.379
4.5



LNU48
24802.2
0.600
0.009
18.1



LNU48
24804.4
0.578
0.015
13.9



LNU8
25063.1
0.640
0.004
26.0



LNU94
24833.3
0.561
0.109
10.5



CONTROL
—
0.573
—
0.0



LNU1
24684.1
0.638
0.209
11.2



LNU1
24681.3
0.625
0.192
9.1



LNU1
24682.2
0.617
0.679
7.6



LNU1
24681.1
0.597
0.605
4.1



LNU133
24744.3
0.624
0.454
8.9



LNU133
24741.1
0.589
0.728
2.7



LNU133
24742.2
0.587
0.795
2.4



LNU175
24732.4
0.666
0.280
16.2



LNU175
24732.1
0.613
0.486
6.9



LNU175
24731.2
0.596
0.623
4.0



LNU175
24734.4
0.592
0.710
3.3



LNU175
24733.4
0.586
0.713
2.3



LNU178
14611.5
0.692
0.028
20.7



LNU178
14611.4
0.624
0.288
8.9



LNU178
14614.5
0.616
0.404
7.4



LNU178
14611.1
0.610
0.435
6.4



LNU178
14612.1
0.587
0.696
2.5



LNU215
24661.4
0.639
0.125
11.5



LNU215
24664.3
0.634
0.227
10.6



LNU24
24973.1
0.626
0.223
9.2



LNU24
24971.4
0.608
0.532
6.1



LNU24
24971.3
0.602
0.471
5.0



LNU6
24992.3
0.639
0.244
11.4



LNU6
24994.2
0.594
0.617
3.6



LNU82
24824.3
0.596
0.551
4.0



LNU82
24824.1
0.596
0.664
4.0



LNU82
24823.1
0.596
0.623
3.9



LNU9
25001.1
0.648
0.159
13.1



LNU9
25003.1
0.647
0.066
12.9



LNU9
25001.2
0.624
0.297
8.9



LNU9
25001.3
0.592
0.636
3.3



CONTROL
—
0.676
—
0.0



LNU120
25463.7
0.756
0.081
11.7



LNU120
25463.3
0.689
0.770
1.9



LNU132
14102.9
0.708
0.416
4.7



LNU132
14102.7
0.690
0.752
2.1



LNU140
14112.7
0.723
0.288
7.0



LNU140
14112.6
0.690
0.737
2.0



LNU180
24724.3
0.760
0.046
12.4



CONTROL
—
0.559
—
0.0



LNU1
24681.1
0.707
0.009
26.5



LNU1
24682.1
0.698
0.025
24.8



LNU1
24683.2
0.628
0.204
12.4



LNU1
24684.1
0.578
0.720
3.3



LNU110
24953.2
0.682
0.044
22.0



LNU110
24952.3
0.593
0.566
6.0



LNU110
24954.3
0.589
0.576
5.4



LNU175
24734.4
0.670
0.058
19.9



LNU175
24732.2
0.645
0.220
15.4



LNU175
24732.1
0.607
0.424
8.5



LNU175
24733.1
0.602
0.491
7.7



LNU19
25151.1
0.607
0.536
8.6



LNU215
24663.4
0.729
0.007
30.4



LNU215
24661.4
0.699
0.013
24.9



LNU215
24664.2
0.683
0.040
22.2



LNU215
24663.3
0.668
0.126
19.5



LNU215
24663.1
0.632
0.187
13.1



LNU27
24873.1
0.662
0.093
18.4



LNU27
24873.4
0.631
0.234
12.9



LNU27
24871.4
0.575
0.763
2.9



LNU44
24924.2
0.724
0.009
29.4



LNU44
24922.3
0.717
0.013
28.3



LNU44
24923.3
0.668
0.073
19.5



LNU44
24924.3
0.610
0.456
9.1



LNU54
24901.2
0.786
0.002
40.5



LNU54
24902.4
0.733
0.003
31.2



LNU54
24903.3
0.653
0.227
16.8



LNU54
24902.7
0.645
0.151
15.3



LNU54
24903.5
0.634
0.241
13.4



LNU79
24882.2
0.682
0.026
22.0



LNU79
24881.1
0.679
0.057
21.4



LNU79
24884.4
0.675
0.041
20.7



LNU79
24884.3
0.626
0.303
12.1



LNU79
24883.2
0.604
0.426
8.0



CONTROL
—
0.579
—
0.0



LNU109
24892.8
0.635
0.116
9.7



LNU109
24892.6
0.608
0.476
5.0



LNU109
24891.2
0.593
0.755
2.4



LNU110
24952.1
0.729
0.049
25.9



LNU110
24952.3
0.624
0.341
7.9



LNU133
24741.1
0.641
0.157
10.7



LNU133
24744.3
0.628
0.417
8.5



LNU19
25151.1
0.660
0.070
13.9



LNU27
24873.4
0.675
0.052
16.6



LNU44
24922.3
0.654
0.110
13.0



LNU54
24901.2
0.711
0.036
22.7



LNU6
24992.3
0.662
0.126
14.3



LNU6
24994.5
0.617
0.380
6.6



LNU79
24881.1
0.642
0.082
10.9



LNU79
24884.4
0.622
0.408
7.5



LNU79
24882.2
0.598
0.637
3.3



LNU79
24883.2
0.590
0.762
1.9



CONTROL
—
0.530
—
0.0



LNU109
24892.8
0.658
0.032
24.3



LNU109
24891.2
0.573
0.550
8.1



LNU109
24891.5
0.552
0.715
4.2



LNU143
25972.1
0.665
0.035
25.5



LNU143
25975.3
0.619
0.137
16.8



LNU143
25971.2
0.593
0.274
12.0



LNU143
25971.5
0.588
0.323
11.0



LNU154
14604.6
0.619
0.131
16.9



LNU154
14602.8
0.591
0.440
11.6



LNU154
14604.7
0.588
0.371
11.0



LNU196
25532.2
0.675
0.013
27.4



LNU196
25534.1
0.645
0.113
21.9



LNU196
25533.1
0.596
0.226
12.6



LNU196
25532.1
0.554
0.782
4.5



LNU207
24642.4
0.656
0.046
23.8



LNU207
24644.18
0.593
0.272
12.0



LNU207
24642.5
0.557
0.654
5.2



LNU207
24644.13
0.546
0.800
3.1



LNU288
14562.7
0.624
0.142
17.8



LNU288
14564.9
0.602
0.177
13.6



LNU288
14562.1
0.586
0.349
10.6



LNU288
14562.9
0.568
0.454
7.2



LNU50
26024.2
0.615
0.205
16.1



LNU50
26023.2
0.603
0.254
13.9



LNU50
26023.5
0.586
0.290
10.6



LNU50
26025.4
0.580
0.453
9.6



LNU52
25723.2
0.681
0.034
28.6



LNU52
25721.4
0.604
0.276
14.1



LNU52
25721.3
0.566
0.561
6.9



CONTROL
—
0.496
—
0.0



LNU143
25975.2
0.614
0.010
23.7



LNU143
25971.2
0.600
0.007
20.9



LNU143
25971.5
0.577
0.071
16.3



LNU143
25972.1
0.571
0.096
15.1



LNU143
25975.3
0.560
0.056
12.9



LNU154
14602.8
0.583
0.030
17.5



LNU154
14601.6
0.556
0.146
12.1



LNU207
24642.5
0.651
0.005
31.2



LNU207
24641.1
0.636
0.005
28.2



LNU207
24642.4
0.582
0.026
17.3



LNU211
24771.1
0.631
0.009
27.3



LNU211
24774.4
0.557
0.286
12.2



LNU211
24771.3
0.546
0.192
10.1



LNU52
25723.1
0.635
0.008
28.1



LNU52
25721.2
0.632
0.002
27.4



LNU52
25721.1
0.605
0.030
22.0



LNU52
25723.2
0.531
0.460
7.1



LNU69
14571.1
0.653
0.004
31.7



LNU69
14573.3
0.603
0.037
21.6



LNU69
14572.9
0.583
0.014
17.6



LNU69
14572.8
0.565
0.051
14.0



LNU69
14573.7
0.520
0.567
4.8



CONTROL
—
0.489
—
0.0



LNU150
24841.9
0.673
0.034
37.7



LNU150
24842.9
0.631
0.096
29.1



LNU150
24843.5
0.533
0.611
9.1



LNU179
24631.6
0.674
0.032
38.1



LNU179
24631.9
0.673
0.037
37.8



LNU179
24632.5
0.608
0.166
24.5



LNU179
24631.7
0.598
0.193
22.5



LNU179
24632.7
0.535
0.585
9.4



LNU232
26001.5
0.572
0.345
17.1



LNU232
26003.7
0.568
0.349
16.3



LNU232
26003.3
0.561
0.394
14.9



LNU232
26001.2
0.552
0.454
13.1



LNU232
26003.6
0.523
0.689
7.0



LNU235
26185.3
0.674
0.037
37.9



LNU235
26184.4
0.628
0.114
28.6



LNU235
26185.2
0.611
0.146
25.2



LNU235
26184.2
0.606
0.175
24.0



LNU235
26182.1
0.556
0.429
13.9



LNU242
25474.1
0.646
0.076
32.2



LNU242
25473.1
0.635
0.095
30.1



LNU242
25473.3
0.596
0.205
22.0



LNU242
25471.1
0.542
0.524
11.0



LNU76
26423.1
0.676
0.030
38.5



LNU76
26425.1
0.645
0.069
32.0



LNU76
26421.2
0.605
0.193
23.9



LNU76
26421.1
0.568
0.383
16.2



LNU95
13985.11
0.663
0.046
35.7



LNU95
13985.15
0.632
0.092
29.3



LNU95
13985.19
0.628
0.115
28.5



LNU95
13985.12
0.565
0.375
15.7



LNU95
13985.16
0.565
0.369
15.6



CONTROL
—
0.552
—
0.0



LNU118
14012.15
0.580
0.661
5.0



LNU150
24843.5
0.647
0.138
17.1



LNU150
24841.6
0.576
0.712
4.3



LNU150
24841.9
0.571
0.776
3.3



LNU179
24632.5
0.600
0.471
8.6



LNU179
24631.7
0.583
0.640
5.5



LNU179
24631.6
0.572
0.762
3.4



LNU232
26003.3
0.600
0.461
8.5



LNU232
26001.5
0.581
0.672
5.2



LNU235
26185.2
0.653
0.151
18.2



LNU235
26184.4
0.648
0.155
17.2



LNU235
26184.2
0.618
0.309
11.8



LNU242
25474.1
0.608
0.403
10.1



LNU288
14562.7
0.622
0.286
12.7



LNU288
14564.9
0.608
0.389
10.1



LNU288
14563.9
0.607
0.402
9.8



LNU288
14563.6
0.595
0.502
7.8



LNU288
14562.1
0.588
0.576
6.4



LNU76
26423.1
0.635
0.217
15.0



LNU76
26421.2
0.623
0.279
12.8



LNU76
26422.2
0.602
0.443
9.0



LNU95
13985.16
0.657
0.123
19.0



LNU95
13985.15
0.621
0.282
12.4



LNU95
13985.11
0.594
0.519
7.5



CONTROL
—
0.580
—
0.0



LNU101
27632.7
0.664
0.135
14.5



LNU128
26511.5
0.645
0.229
11.2



LNU128
26515.3
0.594
0.788
2.5



LNU192
28315.2
0.675
0.088
16.5



LNU192
28313.2
0.671
0.114
15.7



LNU192
28312.2
0.645
0.212
11.3



LNU192
28313.3
0.606
0.632
4.6



LNU206
27622.1
0.648
0.211
11.8



LNU211
24771.1
0.745
0.004
28.5



LNU282
27562.1
0.686
0.067
18.3



LNU282
27563.3
0.667
0.138
15.1



LNU282
27563.1
0.653
0.174
12.6



LNU282
27565.2
0.616
0.513
6.2



LNU69
14571.1
0.706
0.020
21.7



LNU69
14573.5
0.610
0.578
5.2



LNU75
27572.2
0.616
0.530
6.2



LNU75
27572.1
0.613
0.539
5.8



CONTROL
—
0.556
—
0.0



LNU101
27632.5
0.605
0.353
8.7



LNU101
27632.6
0.585
0.555
5.2



LNU118
14012.15
0.655
0.047
17.7



LNU118
14012.12
0.604
0.370
8.6



LNU128
26515.3
0.617
0.231
10.9



LNU128
26511.5
0.591
0.498
6.2



LNU206
27622.4
0.676
0.029
21.6



LNU206
27621.2
0.640
0.181
15.0



LNU206
27622.1
0.605
0.401
8.7



LNU249
26154.2
0.597
0.477
7.4



LNU249
26151.1
0.589
0.576
5.8



LNU249
26152.4
0.588
0.631
5.8



LNU249
26152.2
0.573
0.726
3.0



LNU282
27563.1
0.639
0.143
14.9



LNU282
27565.2
0.629
0.151
13.0



LNU282
27563.3
0.595
0.477
7.0



LNU282
27562.1
0.582
0.633
4.6



LNU288
14562.7
0.708
0.006
27.2



LNU288
14563.6
0.652
0.069
17.2



LNU288
14562.9
0.645
0.068
16.0



LNU288
14563.9
0.631
0.178
13.4



LNU288
14564.8
0.583
0.608
4.9



LNU75
27572.2
0.725
0.003
30.4



LNU75
27571.4
0.652
0.063
17.2



LNU75
27572.3
0.636
0.111
14.3



LNU75
27571.2
0.627
0.179
12.8



CONTROL
—
0.596
—
0.0



LNU14
27821.3
0.619
0.681
3.9



LNU183
24863.1
0.615
0.740
3.2



LNU201
28222.2
0.639
0.479
7.3



LNU201
28223.1
0.615
0.747
3.1



LNU201
28222.3
0.613
0.765
2.9



LNU268
26041.6
0.661
0.241
10.8



LNU268
26044.2
0.647
0.386
8.6



LNU268
26045.1
0.619
0.666
3.9



CONTROL
—
0.607
—
0.0



LNU11
28205.2
0.732
0.021
20.7



LNU11
28202.5
0.650
0.304
7.2



LNU11
28205.1
0.640
0.404
5.6



LNU112
28212.4
0.669
0.188
10.3



LNU112
28212.1
0.637
0.527
5.0



LNU14
27821.3
0.678
0.147
11.8



LNU14
27821.1
0.637
0.469
5.0



LNU14
27824.2
0.628
0.661
3.6



LNU14
27821.4
0.621
0.752
2.5



LNU183
24864.6
0.773
0.000
27.4



LNU183
24863.12
0.683
0.164
12.6



LNU183
24863.1
0.669
0.163
10.2



LNU191
28325.4
0.659
0.293
8.7



LNU191
28321.3
0.645
0.407
6.4



LNU191
28324.2
0.627
0.654
3.4



LNU191
28323.1
0.621
0.727
2.4



LNU201
28222.2
0.735
0.009
21.1



LNU201
28223.3
0.668
0.214
10.2



LNU201
28223.1
0.662
0.256
9.1



LNU201
28221.3
0.624
0.731
2.8



LNU268
26041.6
0.694
0.059
14.4



LNU268
26041.4
0.690
0.066
13.8



LNU268
26043.4
0.623
0.696
2.7



CONTROL
—
0.580
—
0.0



LNU107
14584.9
0.616
0.595
6.3



LNU116
14492.5
0.654
0.142
12.8



LNU116
14492.9
0.644
0.255
11.0



LNU116
14494.5
0.610
0.603
5.2



LNU121
27711.1
0.715
0.026
23.3



LNU121
25642.2
0.629
0.325
8.5



LNU121
27713.4
0.613
0.581
5.7



LNU126
25345.1
0.612
0.569
5.4



LNU126
25343.1
0.598
0.765
3.1



LNU158
27433.3
0.652
0.199
12.3



LNU158
27433.2
0.647
0.220
11.5



LNU158
27432.5
0.644
0.297
11.0



LNU158
27434.1
0.614
0.523
5.9



LNU177
24764.9
0.642
0.365
10.7



LNU177
24762.6
0.628
0.398
8.2



LNU177
24764.12
0.620
0.512
6.9



LNU182
25384.1
0.699
0.065
20.5



LNU182
27521.4
0.611
0.610
5.3



LNU225
25991.5
0.657
0.230
13.2



LNU225
25991.8
0.648
0.196
11.8



LNU225
25991.2
0.615
0.602
6.0



LNU225
25991.3
0.603
0.668
4.0



LNU239
26284.2
0.597
0.754
3.0



LNU57
27854.5
0.618
0.456
6.6



LNU57
27852.1
0.615
0.577
6.0



LNU83
27682.1
0.677
0.105
16.8



LNU83
27681.4
0.631
0.462
8.7



CONTROL
—
0.620
—
0.0



LNU107
14584.9
0.711
0.447
14.8



LNU107
14585.2
0.678
0.597
9.5



LNU107
14583.8
0.655
0.757
5.6



LNU116
14492.5
0.689
0.531
11.2



LNU121
27711.1
0.703
0.460
13.5



LNU121
27713.1
0.702
0.488
13.2



LNU121
25642.2
0.653
0.776
5.4



LNU126
25343.1
0.688
0.580
11.1



LNU126
25343.4
0.652
0.767
5.1



LNU158
27433.3
0.695
0.530
12.1



LNU158
27432.5
0.687
0.570
10.9



LNU158
27434.5
0.655
0.768
5.7



LNU177
24763.6
0.662
0.710
6.9



LNU177
24765.2
0.659
0.732
6.4



LNU177
24764.12
0.652
0.778
5.3



LNU182
25384.2
0.653
0.764
5.4



LNU2
27842.3
0.709
0.460
14.4



LNU2
27842.1
0.703
0.466
13.5



LNU225
25991.3
0.666
0.693
7.5



LNU225
25991.2
0.656
0.747
5.9



LNU225
25991.8
0.655
0.756
5.8



LNU225
25991.1
0.650
0.791
5.0



LNU57
27854.3
0.693
0.520
11.9



LNU57
27852.1
0.680
0.615
9.7



LNU57
27854.5
0.652
0.779
5.2



LNU83
27685.1
0.718
0.421
15.9



LNU83
27685.2
0.710
0.446
14.6



LNU83
27681.4
0.661
0.722
6.7







Table 67.













TABLE 68







Genes showing improved plant growth rate at nitrogen


deficient conditions (T1 generation)













RGR Of



RGR Of Leaf Area

Roots Coverage














Gene

p-
%


p-
%


Name
Ave.
value
incr.
Gene Name
Ave.
value
incr.

















CONT.
0.057
—
0.0
CONT.
0.440
—
0.0


LNU121
0.059
0.705
3.5
LNU121
0.620
0.003
41.0


LNU154
0.061
0.457
6.5
LNU150
0.464
0.668
5.4


CONT.
0.067
—
0.0
LNU154
0.456
0.793
3.7


LNU188
0.070
0.724
5.4
CONT.
0.585
—
0.0


LNU2
0.070
0.703
4.4
LNU127
0.717
0.182
22.6


LNU255
0.068
0.888
1.6
LNU188
0.639
0.589
9.2


LNU265
0.067
0.946
0.8
LNU217
0.616
0.741
5.3


LNU58
0.078
0.290
16.5
LNU239
0.652
0.570
11.4


LNU83
0.072
0.600
7.7
LNU265
0.766
0.076
30.9


CONT.
0.059
—
0.0
LNU275
1.396
0.000
138.7


LNU243
0.061
0.723
3.8
LNU32
0.643
0.615
9.9


LNU262
0.062
0.532
5.9
LNU57
0.714
0.134
22.1


LNU60
0.062
0.618
5.7
LNU58
1.365
0.000
133.3






LNU83
0.754
0.094
28.9






CONT.
0.423
—
0.0






LNU176
0.490
0.470
15.9






LNU214
0.524
0.228
23.9






LNU223
0.460
0.658
8.6






LNU233
0.529
0.217
25.1






LNU245
0.450
0.741
6.3






LNU247
0.671
0.019
58.6






LNU284
0.571
0.166
34.9






LNU289
0.574
0.115
35.6






LNU70
0.457
0.700
8.1






LNU85
0.500
0.436
18.1






CONT.
0.541
—
0.0






LNU225
0.661
0.108
22.2






LNU262
0.587
0.477
8.5






LNU266
0.606
0.382
12.1






LNU29
0.553
0.840
2.3






LNU51
0.557
0.826
3.0






LNU60
0.779
0.008
44.1






CONT.
0.454
—
0.0






LNU171
0.767
0.04 
70.0






LNU222_H6
0.4711
0.88 
4.0





Table 68.


“CONT.”—Control;


“Ave.”—Average;


“% Incr.” = % increment.













TABLE 69







Genes showing improved plant growth rate at nitrogen


deficient conditions (T1 generation)









RGR Of Roots Length













Gene Name
Event #
Average
p-value
% increment

















CONTROL
—
0.477
—
0.0



LNU121
27711
0.512
0.373
7.2



LNU154
14601
0.501
0.551
5.0



LNU68
14031
0.498
0.586
4.3



CONTROL
—
0.542
—
0.0



LNU127
27601
0.545
0.939
0.6



LNU217
28231
0.576
0.469
6.3



LNU265
30261
0.548
0.886
1.2



LNU275
30341
0.645
0.030
19.0



LNU57
27851
0.595
0.226
9.8



LNU58
27671
0.803
0.000
48.1



LNU83
27681
0.626
0.073
15.5



CONTROL
—
0.462
—
0.0



LNU176
29501
0.472
0.815
2.2



LNU214
29521
0.463
0.960
0.3



LNU223
29561
0.483
0.503
4.6



LNU233
29461
0.486
0.538
5.2



LNU245
29831
0.462
0.996
0.0



LNU247
29571
0.521
0.147
12.9



LNU289
29741
0.485
0.579
5.1



LNU70
29591
0.487
0.460
5.6



LNU85
29551
0.504
0.380
9.2



CONTROL
—
0.551
—
0.0



LNU60
29991
0.590
0.365
7.1







Table 69.






The genes listed in Tables 70, 71, 72 and 73 improved plant NUE when grown at standard nitrogen concentration levels. These genes produced larger plant biomass (plant fresh and dry weight and leaf area) when grown under standard nitrogen growth conditions, compared to control plants. Larger plant biomass under this growth conditions indicates the high ability of the plant to better metabolize the nitrogen present in the medium. The genes were cloned under the regulation of a constitutive promoter (At6669) or root preferred promoter (RootP). The evaluation of each gene was performed by testing the performance of different number of events. Some of the genes were evaluated in more than one tissue culture assay and the results obtained where positive as well. Event with p-value <0.1 was considered statistically significant







TABLE 70







Genes showing improved plant performance at standard nitrogen growth conditions


(T2 generation)











Plant Biomass Fresh

Plant Biomass Dry



weight [gr.]

Weight [gr.]
















Gene


p-
%
Gene


p-
%


Name
Event #
Ave.
value
incr.
Name
Event #
Ave.
value
incr.



















CONT.
—
0.130
—
0.0
CONT.
—
0.005
—
0.0


LNU100
14474.3
0.199
0.219
52.9
LNU100
14474.3
0.010
0.123
102.5


LNU100
14474.4
0.137
0.727
5.3
LNU100
14474.4
0.006
0.378
23.5


LNU104
25034.1
0.184
0.296
41.6
LNU104
25033.3
0.007
0.224
39.5


LNU104
25033.3
0.170
0.199
30.9
LNU213
24654.4
0.011
0.107
108.3


LNU213
24654.4
0.228
0.080
75.1
LNU213
24653.2
0.011
0.003
104.9


LNU213
24653.2
0.212
0.012
62.6
LNU213
24651.1
0.007
0.073
36.1


LNU213
24651.1
0.160
0.114
22.6
LNU218
24783.2
0.012
0.100
124.8


LNU218
24783.2
0.239
0.098
84.1
LNU218
24781.7
0.007
0.089
28.4


LNU218
24781.7
0.156
0.144
19.7
LNU4
25134.1
0.007
0.070
38.6


LNU4
25134.1
0.165
0.065
26.5
LNU48
24803.2
0.008
0.139
47.3


LNU48
24803.2
0.177
0.060
35.7
LNU48
24802.2
0.007
0.102
32.3


LNU48
24802.2
0.155
0.420
19.2
LNU48
24804.4
0.006
0.382
18.2


LNU48
24804.4
0.138
0.716
5.7
LNU8
25063.1
0.008
0.045
63.3


LNU8
25063.1
0.192
0.136
47.6
LNU8
25062.2
0.007
0.183
44.9


LNU8
25062.2
0.173
0.236
32.8
LNU8
25063.6
0.006
0.207
19.2


LNU8
25063.6
0.140
0.548
7.6
LNU94
24833.3
0.006
0.234
19.7


CONT.
—
0.115
—
0.0
LNU94
24834.1
0.006
0.607
11.4


LNU1
24681.3
0.162
0.123
40.8
CONT.
—
0.006
—
0.0


LNU1
24682.2
0.125
0.542
9.1
LNU1
24681.3
0.009
0.141
44.4


LNU1
24684.1
0.122
0.376
6.3
LNU1
24682.2
0.007
0.548
12.0


LNU1
24682.1
0.121
0.387
5.3
LNU133
24741.1
0.015
0.046
142.4


LNU133
24741.1
0.264
0.035
130.1
LNU133
24744.3
0.014
0.033
120.8


LNU133
24744.3
0.238
0.054
107.4
LNU175
24732.1
0.011
0.086
68.4


LNU175
24734.4
0.179
0.058
55.6
LNU175
24734.4
0.010
0.067
52.4


LNU175
24732.1
0.173
0.101
50.8
LNU175
24732.4
0.008
0.264
33.2


LNU175
24732.4
0.155
0.181
35.2
LNU175
24731.2
0.007
0.367
16.0


LNU175
24731.2
0.145
0.100
26.7
LNU178
14614.5
0.009
0.121
39.2


LNU178
14614.5
0.153
0.109
32.8
LNU178
14611.5
0.009
0.060
37.2


LNU178
14611.5
0.148
0.091
28.6
LNU178
14611.1
0.007
0.505
10.0


LNU178
14611.1
0.134
0.313
16.7
LNU215
24664.3
0.013
0.002
104.8


LNU215
24664.3
0.231
0.004
100.8
LNU215
24661.4
0.007
0.498
13.6


LNU215
24661.4
0.136
0.260
18.1
LNU24
24973.1
0.010
0.156
67.2


LNU24
24971.4
0.178
0.025
55.3
LNU24
24971.4
0.009
0.034
40.8


LNU24
24973.1
0.172
0.126
50.1
LNU24
24971.2
0.007
0.253
19.2


LNU24
24971.2
0.135
0.129
17.7
LNU6
24992.3
0.016
0.075
150.4


LNU6
24992.3
0.308
0.086
168.0
LNU82
24823.1
0.010
0.231
63.2


LNU6
24993.3
0.135
0.561
17.3
LNU9
25001.3
0.011
0.004
81.6


LNU6
24994.1
0.123
0.466
7.1
LNU9
25001.1
0.007
0.657
5.6


LNU6
24994.5
0.118
0.679
3.0
CONT.
—
0.004
—
0.0


LNU82
24823.1
0.198
0.212
72.8
LNU120
2546.37
0.007
0.030
71.6


LNU9
25001.3
0.201
0.011
75.4
LNU120
25463.3
0.005
0.152
29.7


LNU9
25001.1
0.128
0.321
11.2
LNU120
25463.6
0.005
0.070
22.6


CONT.
—
0.090
—
0.0
LNU124
14501.7
0.006
0.008
65.2


LNU120
25463.7
0.144
0.021
60.4
LNU124
14501.1
0.005
0.070
35.5


LNU120
25463.3
0.126
0.003
40.2
LNU124
14502.7
0.004
0.578
5.8


LNU120
25463.6
0.120
0.279
33.0
LNU132
14102.7
0.005
0.136
34.2


LNU120
25464.1
0.095
0.707
5.6
LNU132
14101.9
0.004
0.507
12.9


LNU124
14501.7
0.151
0.006
68.2
LNU132
14102.9
0.004
0.330
9.0


LNU124
14501.1
0.132
0.065
47.0
LNU132
14102.6
0.004
0.650
5.2


LNU124
14502.1
0.117
0.342
30.1
LNU140
14112.7
0.007
0.192
72.3


LNU124
14502.7
0.097
0.521
7.9
LNU140
14111.6
0.005
0.301
20.0


LNU132
14102.7
0.134
0.043
48.6
LNU180
24724.3
0.009
0.010
124.5


LNU132
14102.9
0.109
0.119
20.7
LNU180
24723.3
0.006
0.280
47.7


LNU132
14101.9
0.106
0.158
18.3
LNU196
25534.1
0.006
0.024
63.9


LNU140
14112.7
0.142
0.291
58.4
LNU196
25533.3
0.005
0.509
18.7


LNU140
14111.6
0.117
0.190
29.7
LNU196
25533.1
0.005
0.110
16.8


LNU140
14114.8
0.097
0.690
8.4
LNU20
24933.2
0.005
0.323
34.8


LNU180
24724.3
0.172
0.000
91.7
LNU36
25562.3
0.006
0.034
47.7


LNU180
24723.3
0.148
0.172
64.5
LNU36
25562.4
0.005
0.040
31.0


LNU180
24721.4
0.100
0.586
11.3
LNU36
25561.2
0.004
0.628
3.9


LNU180
24724.1
0.096
0.736
7.2
LNU71
25853.4
0.007
0.004
92.9


LNU196
25534.1
0.141
0.012
56.4
LNU71
25852.4
0.005
0.206
23.9


LNU196
25533.3
0.114
0.448
27.2
CONT.
—
0.004
—
0.0


LNU196
25533.1
0.102
0.223
13.9
LNU1
24681.3
0.008
0.000
108.6


LNU20
24933.2
0.132
0.237
46.5
LNU1
24683.2
0.004
0.244
15.5


LNU20
24933.1
0.094
0.794
4.7
LNU110
24953.3
0.005
0.197
39.9


LNU36
25562.3
0.134
0.001
48.8
LNU110
24952.3
0.005
0.321
35.9


LNU36
25562.4
0.130
0.212
44.1
LNU175
24732.2
0.009
0.151
132.3


LNU36
25561.2
0.095
0.689
5.1
LNU175
24733.4
0.007
0.054
98.4


LNU71
25853.4
0.165
0.001
83.4
LNU175
24732.1
0.004
0.537
13.5


LNU71
25852.4
0.147
0.020
63.7
LNU175
24734.4
0.004
0.573
8.0


CONT.
—
0.125
—
0.0
LNU19
25151.1
0.008
0.031
107.2


LNU1
24681.3
0.180
0.092
44.5
LNU19
25153.3
0.006
0.059
52.9


LNU110
24952.3
0.148
0.419
18.6
LNU19
25153.1
0.004
0.328
10.1


LNU175
24732.2
0.199
0.237
59.6
LNU215
24663.4
0.013
0.209
259.4


LNU175
24733.4
0.177
0.071
41.9
LNU215
24663.1
0.007
0.365
95.0


LNU19
25151.1
0.185
0.230
48.6
LNU215
24663.3
0.005
0.123
30.4


LNU215
24663.4
0.203
0.008
63.3
LNU215
24664.2
0.004
0.411
17.5


LNU215
24663.3
0.134
0.681
7.9
LNU27
24873.1
0.009
0.000
131.0


LNU27
24873.1
0.210
0.005
68.6
LNU27
24873.4
0.005
0.420
29.1


LNU27
24873.4
0.152
0.570
21.9
LNU44
24924.2
0.021
0.318
464.5


LNU44
24924.3
0.207
0.068
66.3
LNU44
24924.3
0.009
0.095
140.5


LNU44
24923.1
0.150
0.444
20.2
LNU44
24923.1
0.006
0.185
69.2


LNU44
24924.2
0.138
0.646
10.6
LNU44
24922.3
0.005
0.387
26.4


LNU54
24903.5
0.191
0.146
53.5
LNU54
24903.5
0.008
0.035
107.9


LNU54
24903.3
0.145
0.354
16.1
LNU54
24903.3
0.006
0.010
58.3


LNU54
24901.2
0.138
0.645
11.0
LNU79
24881.1
0.008
0.016
119.4


LNU79
24884.4
0.212
0.005
70.5
LNU79
24884.4
0.008
0.062
112.6


LNU79
24881.1
0.177
0.036
41.9
LNU79
24884.3
0.005
0.166
47.4


LNU79
24884.3
0.167
0.173
33.8
LNU79
24882.2
0.004
0.169
18.2


CONT.
—
0.132
—
0.0
CONT.
—
0.006
—
0.0


LNU109
24891.2
0.198
0.021
49.7
LNU109
24891.2
0.010
0.003
69.3


LNU109
24892.6
0.180
0.074
36.2
LNU109
24892.6
0.009
0.113
41.8


LNU109
24891.5
0.165
0.048
25.0
LNU109
24891.5
0.009
0.109
39.3


LNU109
24892.5
0.160
0.367
20.9
LNU109
24892.5
0.008
0.290
26.2


LNU110
24952.1
0.241
0.008
81.9
LNU110
24952.1
0.012
0.000
100.4


LNU110
24953.2
0.208
0.030
57.3
LNU110
24954.1
0.010
0.019
66.4


LNU110
24954.1
0.194
0.011
46.8
LNU110
24953.2
0.010
0.013
63.1


LNU110
24952.3
0.183
0.046
38.1
LNU110
24952.3
0.009
0.061
54.5


LNU110
24954.3
0.142
0.445
7.7
LNU110
24954.3
0.008
0.030
25.8


LNU133
24741.2
0.249
0.014
88.2
LNU133
24741.2
0.014
0.019
127.0


LNU133
24744.3
0.232
0.011
75.6
LNU133
24741.1
0.011
0.004
82.4


LNU133
24741.1
0.229
0.010
73.5
LNU133
24744.3
0.011
0.033
76.2


LNU133
24742.2
0.199
0.123
50.5
LNU133
24744.2
0.010
0.015
68.0


LNU133
24744.2
0.186
0.055
40.8
LNU133
24742.2
0.010
0.019
63.1


LNU19
25151.1
0.253
0.026
91.0
LNU19
25151.1
0.012
0.020
104.5


LNU19
25153.3
0.166
0.349
25.3
LNU19
25153.3
0.009
0.158
43.4


LNU27
24873.4
0.293
0.029
121.4
LNU19
25151.11
0.006
0.617
3.3


LNU27
24871.4
0.159
0.476
20.6
LNU27
24873.4
0.015
0.032
150.8


LNU44
24922.3
0.218
0.021
64.5
LNU27
24871.4
0.007
0.420
20.1


LNU44
24923.1
0.153
0.200
15.6
LNU27
24873.1
0.006
0.737
3.7


LNU44
24924.3
0.151
0.470
14.4
LNU44
24922.3
0.011
0.009
84.0


LNU44
24923.3
0.139
0.676
5.3
LNU44
24923.1
0.008
0.058
27.9


LNU54
24903.5
0.286
0.027
116.3
LNU44
24924.3
0.008
0.214
27.0


LNU54
24902.4
0.221
0.294
67.4
LNU54
24903.5
0.015
0.022
142.6


LNU54
24901.2
0.159
0.479
19.9
LNU54
24902.4
0.011
0.184
83.6


LNU6
24992.3
0.240
0.066
81.6
LNU54
24901.2
0.009
0.194
43.9


LNU6
24994.5
0.240
0.121
81.6
LNU6
24992.3
0.012
0.082
91.4


LNU6
24994.1
0.166
0.219
25.6
LNU6
24994.5
0.012
0.172
90.2


LNU79
24881.1
0.255
0.038
92.8
LNU6
24994.1
0.009
0.167
40.2


LNU79
24882.2
0.232
0.026
75.6
LNU6
24994.2
0.008
0.345
24.6


LNU79
24884.4
0.226
0.163
70.7
LNU79
24881.1
0.012
0.007
99.6


LNU79
24883.2
0.198
0.041
49.9
LNU79
24882.2
0.012
0.031
94.7


LNU79
24884.3
0.190
0.074
43.8
LNU79
24883.2
0.011
0.011
88.1


CONT.
—
0.119
—
0.0
LNU79
24884.4
0.010
0.083
62.7


LNU109
24892.8
0.195
0.058
63.2
LNU79
24884.3
0.010
0.028
61.1


LNU109
24891.5
0.188
0.135
57.1
CONT.
—
0.005
—
0.0


LNU109
24891.2
0.139
0.570
16.6
LNU109
24892.8
0.010
0.063
89.5


LNU154
14604.7
0.162
0.247
35.9
LNU109
24891.5
0.008
0.243
50.7


LNU154
14604.6
0.131
0.719
9.4
LNU109
24891.2
0.007
0.486
26.5


LNU196
25532.2
0.223
0.133
86.8
LNU143
25975.2
0.007
0.556
21.0


LNU196
25534.1
0.129
0.790
7.6
LNU154
14604.7
0.009
0.192
56.2


LNU207
24642.5
0.226
0.071
89.3
LNU196
25532.2
0.011
0.119
108.7


LNU52
25723.2
0.168
0.216
40.9
LNU196
25534.1
0.006
0.650
16.9


LNU52
25721.4
0.128
0.799
7.2
LNU207
24642.5
0.010
0.121
82.6


CONT.
—
0.128
—
0.0
LNU50
26025.4
0.006
0.785
11.4


LNU154
14601.6
0.171
0.487
33.7
LNU52
25723.2
0.008
0.335
38.8


LNU207
24642.5
0.220
0.093
72.5
LNU52
25721.3
0.006
0.723
16.9


LNU207
24642.4
0.181
0.203
41.6
CONT.
—
0.006
—
0.0


LNU211
24771.1
0.200
0.078
56.5
LNU154
14601.6
0.009
0.459
48.4


LNU52
25721.4
0.221
0.057
73.3
LNU207
24642.5
0.011
0.016
69.6


LNU52
25721.1
0.172
0.133
34.9
LNU207
24642.4
0.009
0.061
41.8


LNU52
25723.1
0.154
0.093
20.9
LNU211
24771.1
0.011
0.128
64.9


LNU69
14571.1
0.224
0.035
75.6
LNU52
25721.4
0.011
0.050
75.5


LNU69
14572.8
0.141
0.441
10.5
LNU52
25721.1
0.008
0.231
33.1


CONT.
—
0.120
—
0.0
LNU52
25723.1
0.007
0.628
5.6


LNU150
24843.9
0.179
0.091
49.0
LNU69
14571.1
0.010
0.057
49.2


LNU150
24841.9
0.178
0.015
47.8
CONT.
—
0.005
—
0.0


LNU150
24843.5
0.167
0.002
38.6
LNU150
24841.9
0.009
0.021
64.5


LNU150
24842.9
0.143
0.103
18.6
LNU150
24843.5
0.008
0.003
52.8


LNU179
24632.5
0.127
0.762
5.6
LNU150
24843.9
0.007
0.017
38.0


LNU179
24631.7
0.126
0.771
4.9
LNU150
24842.9
0.007
0.112
30.5


LNU232
26003.7
0.130
0.584
8.4
LNU232
26003.7
0.007
0.248
23.6


LNU232
26001.5
0.130
0.608
7.8
LNU232
26001.5
0.006
0.712
4.5


LNU242
25474.1
0.167
0.058
38.9
LNU235
26184.4
0.006
0.747
4.5


LNU242
25473.1
0.142
0.301
17.8
LNU242
25474.1
0.008
0.167
43.1


LNU76
26421.2
0.164
0.265
36.3
LNU242
25473.1
0.007
0.191
27.3


LNU76
26421.1
0.158
0.389
31.9
LNU76
26421.1
0.008
0.060
51.5


LNU76
26422.2
0.133
0.607
11.0
LNU76
26421.2
0.008
0.154
40.3


LNU95
13985.11
0.186
0.040
55.1
LNU76
26422.2
0.007
0.226
26.4


LNU95
13985.15
0.139
0.296
15.8
LNU76
26425.1
0.006
0.700
4.5


LNU95
13985.16
0.136
0.281
12.9
LNU95
13985.11
0.009
0.069
76.1


LNU95
13985.12
0.129
0.576
7.3
LNU95
13985.15
0.008
0.146
39.8


LNU95
13985.19
0.128
0.702
6.8
LNU95
13985.19
0.006
0.641
12.9


CONT.
—
0.129
—
0.0
LNU95
13985.16
0.006
0.466
10.1


LNU118
14013.8
0.227
0.027
75.7
CONT.
—
0.006
—
0.0


LNU118
14013.6
0.219
0.057
69.9
LNU118
14013.8
0.011
0.008
80.1


LNU118
14012.12
0.187
0.147
45.1
LNU118
14013.6
0.010
0.015
67.7


LNU118
14012.15
0.179
0.077
38.8
LNU118
14012.12
0.010
0.060
55.3


LNU118
14012.14
0.134
0.788
3.7
LNU118
14012.15
0.009
0.147
38.8


LNU150
24842.9
0.248
0.035
91.7
LNU118
14012.14
0.008
0.152
20.9


LNU150
24841.9
0.161
0.111
24.7
LNU150
24842.9
0.012
0.066
89.8


LNU150
24841.6
0.150
0.150
16.3
LNU150
24841.9
0.008
0.019
30.8


LNU150
24843.5
0.144
0.664
11.6
LNU150
24843.5
0.008
0.389
20.4


LNU179
24632.7
0.186
0.022
44.1
LNU150
24841.6
0.007
0.632
5.9


LNU179
24631.7
0.173
0.184
33.8
LNU179
24632.7
0.011
0.015
73.3


LNU179
24631.9
0.137
0.716
6.3
LNU179
24631.7
0.008
0.235
27.6


LNU232
26001.5
0.187
0.033
44.6
LNU232
26001.5
0.009
0.014
41.2


LNU232
26003.3
0.147
0.238
13.5
LNU232
26003.6
0.007
0.469
9.1


LNU232
26003.6
0.136
0.661
5.6
LNU232
26003.3
0.007
0.439
7.5


LNU235
26184.4
0.278
0.001
115.4
LNU235
26184.4
0.014
0.006
124.7


LNU235
26184.2
0.243
0.004
88.4
LNU235
26185.2
0.011
0.000
77.7


LNU235
26185.2
0.226
0.017
75.1
LNU235
26184.2
0.011
0.000
72.5


LNU235
26182.1
0.137
0.751
6.0
LNU235
26182.1
0.007
0.064
19.6


LNU242
25474.1
0.158
0.238
22.6
LNU242
25474.1
0.007
0.176
13.5


LNU288
14563.9
0.274
0.006
112.2
LNU288
14563.9
0.016
0.002
152.4


LNU288
14562.1
0.196
0.026
51.7
LNU288
14562.1
0.010
0.001
62.9


LNU288
14564.9
0.172
0.261
33.5
LNU288
14564.9
0.008
0.366
28.4


LNU288
14563.6
0.144
0.420
11.2
LNU288
14562.7
0.007
0.580
20.0


LNU288
14562.7
0.143
0.684
10.5
LNU288
14563.6
0.007
0.207
15.9


LNU76
26421.2
0.175
0.261
35.2
LNU76
26421.2
0.008
0.238
30.8


LNU76
26422.2
0.153
0.210
18.4
LNU76
26422.2
0.008
0.325
22.0


LNU76
26423.1
0.142
0.444
9.8
LNU76
26423.1
0.006
0.747
3.9


LNU95
13985.16
0.265
0.008
105.4
LNU95
13985.16
0.014
0.015
117.5


LNU95
13985.12
0.198
0.016
53.2
LNU95
13985.12
0.010
0.052
67.7


LNU95
13985.15
0.183
0.016
41.3
LNU95
13985.15
0.010
0.024
52.5


CONT.
—
0.144
—
0.0
LNU95
13985.19
0.007
0.340
12.7


LNU101
27632.1
0.177
0.162
23.0
CONT.
—
0.007
—
0.0


LNU101
27635.1
0.159
0.359
10.4
LNU101
27635.1
0.008
0.360
9.9


LNU128
26515.3
0.181
0.160
26.1
LNU101
27632.1
0.007
0.800
4.2


LNU128
26515.2
0.161
0.642
12.0
LNU128
26515.3
0.010
0.119
39.9


LNU192
28312.2
0.164
0.707
13.7
LNU128
26515.2
0.009
0.223
34.5


LNU192
28315.2
0.163
0.168
13.6
LNU192
28315.2
0.009
0.052
26.0


LNU192
28313.2
0.151
0.614
5.1
LNU192
28313.2
0.008
0.224
17.8


LNU206
27621.1
0.160
0.460
11.0
LNU206
27621.1
0.007
0.783
6.0


LNU211
24771.1
0.250
0.046
73.5
LNU211
24771.1
0.013
0.024
85.5


LNU211
24773.1
0.182
0.452
26.2
LNU211
24773.1
0.009
0.342
29.5


LNU282
27563.3
0.180
0.036
24.8
LNU282
27563.3
0.009
0.164
32.7


LNU69
14571.1
0.199
0.150
38.2
LNU69
14571.1
0.011
0.056
57.4


LNU69
14573.5
0.148
0.713
2.7
LNU69
14573.5
0.009
0.003
23.5


LNU75
27572.1
0.185
0.012
28.7
LNU69
14572.9
0.007
0.741
2.4


LNU75
27572.2
0.162
0.409
12.3
LNU75
27572.1
0.009
0.020
29.9


CONT.
—
0.122
—
0.0
LNU75
27572.2
0.009
0.095
28.5


LNU101
27632.5
0.184
0.165
50.5
CONT.
—
0.005
—
0.0


LNU118
14013.6
0.199
0.023
62.9
LNU101
27632.5
0.009
0.151
69.4


LNU118
14012.15
0.167
0.037
36.3
LNU118
14013.6
0.010
0.026
87.5


LNU118
14013.9
0.147
0.325
20.0
LNU118
14012.15
0.009
0.029
68.0


LNU206
27621.2
0.203
0.058
66.4
LNU118
14013.9
0.007
0.194
28.5


LNU249
26153.1
0.221
0.052
81.0
LNU118
14013.8
0.006
0.581
15.5


LNU249
26152.4
0.137
0.578
12.4
LNU118
14012.12
0.006
0.582
7.7


LNU282
27563.1
0.199
0.029
63.0
LNU206
27621.2
0.008
0.034
56.8


LNU288
14563.9
0.247
0.017
101.9
LNU206
27621.1
0.006
0.604
9.0


LNU288
14564.8
0.225
0.000
84.2
LNU249
26153.1
0.010
0.027
86.1


LNU288
14562.7
0.175
0.197
43.4
LNU249
26152.4
0.006
0.799
5.3


LNU288
14562.9
0.167
0.185
36.6
LNU282
27563.1
0.009
0.021
68.9


LNU288
14563.6
0.149
0.366
21.9
LNU288
14563.9
0.013
0.001
135.7


LNU75
27571.4
0.216
0.002
76.8
LNU288
14564.8
0.011
0.001
99.1


LNU75
27572.3
0.199
0.029
62.9
LNU288
14562.7
0.009
0.270
68.0


LNU75
27571.2
0.166
0.041
35.9
LNU288
14562.9
0.009
0.100
62.9


LNU75
27572.2
0.165
0.174
35.3
LNU288
14563.6
0.006
0.439
17.9


CONT.
—
0.130
—
0.0
LNU75
27571.4
0.010
0.007
83.8


LNU11
28204.1
0.145
0.596
11.2
LNU75
27572.3
0.008
0.109
57.3


LNU11
28204.3
0.144
0.455
11.0
LNU75
27572.2
0.008
0.162
48.5


LNU183
24863.12
0.180
0.020
38.4
LNU75
27571.2
0.008
0.037
44.8


LNU183
24863.1
0.149
0.202
14.3
CONT.
—
0.006
—
0.0


LNU183
24865.1
0.135
0.742
4.1
LNU11
28204.1
0.007
0.585
6.6


LNU268
26044.2
0.180
0.339
38.8
LNU11
28204.3
0.007
0.685
5.8


CONT.
—
0.110
—
0.0
LNU183
24863.12
0.008
0.306
25.6


LNU11
28205.1
0.150
0.052
35.7
LNU268
26044.2
0.008
0.393
32.8


LNU11
28205.2
0.150
0.041
35.6
CONT.
—
0.005
—
0.0


LNU11
28204.1
0.127
0.270
14.9
LNU11
28205.1
0.007
0.134
30.1


LNU11
28203.2
0.114
0.769
3.7
LNU11
28205.2
0.007
0.117
24.5


LNU11
28204.3
0.114
0.794
3.1
LNU11
28204.3
0.006
0.214
16.2


LNU112
28212.3
0.132
0.202
19.4
LNU11
28203.2
0.006
0.430
10.6


LNU112
28212.4
0.126
0.280
14.2
LNU112
28212.4
0.006
0.448
10.2


LNU14
27821.3
0.164
0.101
49.0
LNU14
27821.3
0.008
0.119
49.1


LNU14
27821.4
0.116
0.729
4.8
LNU183
24865.1
0.012
0.000
118.5


LNU183
24863.1
0.263
0.045
138.7
LNU183
24863.1
0.010
0.002
88.0


LNU183
24865.1
0.254
0.000
130.0
LNU183
24863.12
0.010
0.028
82.4


LNU183
24864.6
0.208
0.017
88.8
LNU183
24864.6
0.009
0.001
69.0


LNU183
24863.12
0.199
0.004
80.5
LNU191
28323.1
0.008
0.147
40.3


LNU191
28324.2
0.154
0.034
39.9
LNU191
28324.2
0.008
0.058
39.4


LNU191
28325.4
0.152
0.015
38.2
LNU191
28325.4
0.007
0.023
34.7


LNU191
28323.1
0.145
0.229
31.4
LNU191
28325.3
0.006
0.413
18.5


LNU191
28325.3
0.136
0.156
23.3
LNU201
28222.2
0.010
0.022
85.6


LNU201
28222.2
0.216
0.005
96.0
LNU201
28221.3
0.007
0.124
25.5


LNU201
28223.3
0.149
0.123
35.0
LNU201
28223.3
0.006
0.741
6.0


LNU201
28221.3
0.135
0.148
22.5
LNU268
26041.4
0.008
0.016
54.2


LNU201
28222.3
0.118
0.612
7.3
LNU268
26043.4
0.007
0.077
30.1


LNU268
26043.4
0.173
0.019
56.8
LNU268
26045.1
0.007
0.334
21.0


LNU268
26041.4
0.168
0.039
52.0
CONT.
—
0.004
—
0.0


LNU268
26045.1
0.144
0.074
30.2
LNU107
14584.9
0.007
0.003
75.5


LNU268
26041.6
0.126
0.498
14.6
LNU107
14585.5
0.006
0.369
45.7


LNU268
26044.2
0.119
0.679
7.6
LNU107
14583.8
0.005
0.059
44.4


CONT.
—
0.079
—
0.0
LNU107
14583.7
0.004
0.660
6.0


LNU107
14584.9
0.159
0.047
100.2
LNU116
14493.6
0.006
0.045
67.5


LNU107
14585.5
0.143
0.183
79.7
LNU116
14494.5
0.006
0.005
51.7


LNU107
14583.8
0.120
0.064
50.6
LNU116
14492.9
0.006
0.015
49.0


LNU107
14583.7
0.094
0.281
18.2
LNU116
14492.5
0.004
0.421
9.9


LNU116
14493.6
0.201
0.106
152.6
LNU12
27371.1
0.004
0.750
4.0


LNU116
14494.5
0.125
0.020
56.9
LNU12
27372.5
0.004
0.737
4.0


LNU116
14492.9
0.124
0.067
55.8
LNU121
25642.2
0.009
0.002
150.3


LNU116
14492.5
0.110
0.012
38.2
LNU121
27713.1
0.007
0.000
88.1


LNU121
25642.2
0.193
0.010
142.4
LNU121
27713.4
0.007
0.017
85.4


LNU121
27713.1
0.168
0.002
111.8
LNU121
27711.1
0.007
0.037
73.5


LNU121
27711.1
0.153
0.000
92.5
LNU126
25345.1
0.008
0.008
99.3


LNU121
27713.4
0.148
0.002
86.8
LNU126
25343.1
0.007
0.071
80.8


LNU126
25345.1
0.163
0.006
104.8
LNU126
25343.3
0.007
0.000
76.2


LNU126
25343.3
0.154
0.020
93.9
LNU126
25343.4
0.004
0.144
17.9


LNU126
25343.1
0.137
0.103
72.4
LNU158
27433.3
0.008
0.010
118.5


LNU126
25343.4
0.101
0.120
27.4
LNU158
27433.2
0.007
0.005
94.0


LNU126
25341.1
0.093
0.472
17.1
LNU158
27432.5
0.005
0.077
43.0


LNU158
27433.3
0.193
0.007
142.3
LNU177
24762.6
0.006
0.055
69.5


LNU158
27433.2
0.145
0.000
82.0
LNU177
24764.9
0.005
0.115
30.5


LNU158
27432.5
0.129
0.011
62.9
LNU177
24764.12
0.004
0.524
17.9


LNU158
27434.1
0.087
0.526
9.9
LNU177
24765.2
0.004
0.540
11.3


LNU177
24762.6
0.142
0.012
79.1
LNU182
25384.1
0.008
0.015
102.6


LNU177
24764.9
0.127
0.003
59.2
LNU182
25384.5
0.006
0.021
68.2


LNU177
24764.12
0.103
0.280
29.4
LNU182
27521.4
0.006
0.177
57.0


LNU177
24765.2
0.098
0.191
23.3
LNU182
25384.2
0.006
0.015
55.0


LNU182
25384.2
0.177
0.063
122.7
LNU2
25713.1
0.006
0.007
67.5


LNU182
25384.1
0.156
0.022
96.2
LNU2
27842.3
0.005
0.059
37.1


LNU182
25384.5
0.155
0.047
95.5
LNU2
27842.1
0.005
0.517
35.8


LNU182
27521.4
0.136
0.225
71.0
LNU225
25991.5
0.008
0.039
109.9


LNU2
25713.1
0.140
0.006
75.9
LNU225
25991.2
0.005
0.097
22.5


LNU2
27842.1
0.127
0.182
60.4
LNU225
25991.1
0.004
0.627
9.9


LNU2
27842.3
0.103
0.058
29.2
LNU225
25991.8
0.004
0.678
8.6


LNU225
25991.5
0.169
0.013
112.9
LNU239
26284.1
0.006
0.118
47.0


LNU225
25991.8
0.134
0.177
68.3
LNU239
26281.1
0.004
0.382
18.5


LNU225
25991.3
0.115
0.264
45.3
LNU57
27854.5
0.005
0.120
37.7


LNU225
25991.2
0.105
0.071
31.6
LNU57
27855.1
0.005
0.110
30.5


LNU225
25991.1
0.097
0.169
22.6
LNU57
27854.3
0.004
0.214
18.5


LNU239
26284.1
0.111
0.154
39.4
LNU83
27681.4
0.005
0.101
27.2


LNU239
26283.2
0.104
0.056
30.3
LNU83
27684.1
0.005
0.092
23.8


LNU239
26281.1
0.102
0.034
28.0
LNU83
27685.1
0.004
0.575
16.6


LNU239
26284.2
0.086
0.431
8.2
CONT.
—
0.005
—
0.0


LNU57
27854.5
0.151
0.015
90.2
LNU107
14584.9
0.012
0.000
135.8


LNU57
27854.3
0.133
0.034
67.1
LNU107
14585.2
0.007
0.067
49.4


LNU57
27855.1
0.119
0.146
49.5
LNU107
14583.8
0.005
0.598
9.0


LNU57
27851.2
0.091
0.383
14.4
LNU107
14585.5
0.005
0.488
8.4


LNU57
27852.1
0.088
0.323
10.2
LNU116
14493.6
0.009
0.144
89.3


LNU83
27685.2
0.121
0.242
52.9
LNU116
14491.5
0.009
0.003
74.9


LNU83
27681.4
0.116
0.066
46.5
LNU116
14492.5
0.008
0.003
71.9


LNU83
27685.1
0.114
0.414
43.8
LNU116
14494.5
0.007
0.202
40.7


LNU83
27684.1
0.104
0.250
30.3
LNU121
27713.4
0.015
0.000
214.6


CONT.
—
0.132
—
0.0
LNU121
27713.1
0.010
0.049
104.1


LNU107
14584.9
0.254
0.000
92.8
LNU121
25642.2
0.007
0.146
42.2


LNU107
14585.2
0.157
0.116
19.2
LNU121
27713.3
0.007
0.042
39.6


LNU116
14491.5
0.191
0.020
45.3
LNU121
27711.1
0.006
0.484
16.6


LNU116
14492.5
0.178
0.032
35.2
LNU126
25343.1
0.005
0.646
9.0


LNU116
14493.6
0.177
0.130
34.3
LNU158
27433.3
0.010
0.007
109.7


LNU116
14494.5
0.146
0.513
10.8
LNU158
27432.5
0.009
0.002
88.2


LNU121
27713.4
0.302
0.000
129.5
LNU158
27433.2
0.006
0.187
28.9


LNU121
27713.1
0.221
0.148
68.0
LNU177
24764.12
0.006
0.192
26.3


LNU121
27713.3
0.189
0.103
43.2
LNU182
25384.6
0.006
0.063
27.4


LNU121
25642.2
0.181
0.269
37.7
LNU182
25384.1
0.006
0.393
18.7


LNU121
27711.1
0.146
0.459
10.7
LNU182
25384.2
0.005
0.497
9.5


LNU126
25343.1
0.145
0.561
9.9
LNU2
27842.3
0.009
0.049
76.0


LNU158
27432.5
0.223
0.000
69.6
LNU2
27845.3
0.006
0.106
27.9


LNU158
27433.3
0.216
0.018
64.2
LNU2
27842.1
0.006
0.353
21.7


LNU158
27433.2
0.151
0.438
14.9
LNU2
25713.1
0.005
0.786
7.9


LNU177
24762.6
0.154
0.335
16.9
LNU225
25991.2
0.015
0.007
206.9


LNU177
24764.12
0.139
0.715
5.3
LNU225
25991.3
0.009
0.088
90.3


LNU182
25384.6
0.139
0.656
5.6
LNU225
25991.8
0.006
0.224
20.7


LNU2
27842.3
0.220
0.023
67.2
LNU239
26281.1
0.006
0.206
26.3


LNU2
27842.1
0.165
0.250
25.4
LNU239
26283.2
0.006
0.056
25.3


LNU2
27845.3
0.162
0.280
23.1
LNU239
26284.2
0.006
0.450
19.7


LNU225
25991.2
0.297
0.008
125.7
LNU57
27852.1
0.007
0.084
48.8


LNU225
25991.3
0.206
0.142
56.2
LNU57
27851.2
0.007
0.026
47.8


LNU225
25991.8
0.155
0.226
17.7
LNU57
27854.5
0.007
0.117
33.5


LNU239
26281.1
0.156
0.176
18.1
LNU83
27685.2
0.008
0.235
59.1


LNU239
26284.2
0.153
0.408
16.1
LNU83
27685.1
0.008
0.089
56.5


LNU239
26283.2
0.139
0.709
5.5
LNU83
27681.4
0.006
0.184
25.8


LNU57
27854.5
0.179
0.112
35.6
CONT.

0.0037




LNU57
27852.1
0.172
0.171
30.2
LNU12
27371.1
0.004
0.749
3.4


LNU57
27851.2
0.171
0.158
29.6
LNU12
27372.5
0.004
0.749
3.4


LNU83
27685.2
0.208
0.139
58.1
CONT.
—
0.0051




LNU83
27685.1
0.155
0.346
17.7
LNU17
13991.1
0.0068
0.04 
32.2


CONT.
—
0.116
—
0.0
LNU17
13991.14
0.0053
0.88 
2.2


LNU17
13991.1
0.137
0.2 
17.7





Table 70.


“CONT.”—Control;


“Ave.”—Average;


“% Incr.” = % increment.













TABLE 71







Genes showing improved plant performance at standard


nitrogen growth conditions (T2 generation)









Leaf Area [cm2]













Gene Name
Event #
Average
p-value
% increment

















CONTROL
—
0.520
—
0.0



LNU100
14474.3
0.909
0.052
75.0



LNU100
14474.4
0.612
0.433
17.7



LNU100
14471.4
0.547
0.605
5.3



LNU104
25033.3
0.792
0.040
52.4



LNU213
24654.4
0.962
0.029
85.0



LNU213
24653.2
0.955
0.001
83.8



LNU213
24651.1
0.658
0.045
26.6



LNU218
24783.2
1.103
0.029
112.3



LNU218
24781.7
0.689
0.037
32.6



LNU218
24781.4
0.539
0.771
3.7



LNU4
25134.1
0.737
0.008
41.7



LNU4
25134.2
0.615
0.188
18.4



LNU48
24803.2
0.798
0.007
53.5



LNU48
24802.2
0.679
0.033
30.6



LNU48
24804.4
0.672
0.056
29.2



LNU8
25063.1
0.823
0.008
58.4



LNU8
25062.2
0.780
0.032
50.0



LNU8
25063.6
0.711
0.017
36.8



LNU8
25061.2
0.537
0.772
3.3



LNU94
24833.3
0.656
0.045
26.2



LNU94
24834.4
0.542
0.751
4.3



LNU94
24834.1
0.540
0.785
3.8



CONTROL
—
0.743
—
0.0



LNU1
24681.3
0.883
0.305
18.9



LNU1
24682.2
0.822
0.490
10.7



LNU1
24682.1
0.783
0.569
5.4



LNU1
24684.1
0.761
0.775
2.5



LNU133
24741.1
1.297
0.027
74.7



LNU133
24744.3
1.285
0.000
73.0



LNU175
24732.1
1.056
0.030
42.2



LNU175
24734.4
1.042
0.041
40.3



LNU175
24732.4
0.874
0.310
17.6



LNU178
14614.5
1.008
0.060
35.7



LNU178
14611.5
0.899
0.050
21.1



LNU178
14611.1
0.774
0.665
4.2



LNU215
24664.3
1.274
0.006
71.5



LNU215
24661.4
0.826
0.308
11.2



LNU24
24973.1
1.027
0.018
38.3



LNU24
24971.4
0.935
0.029
25.9



LNU24
24971.2
0.860
0.152
15.7



LNU6
24992.3
1.411
0.054
90.0



LNU6
24993.3
0.888
0.363
19.6



LNU82
24823.1
1.043
0.214
40.5



LNU9
25001.3
1.094
0.004
47.4



LNU9
25001.1
0.779
0.631
4.9



CONTROL
—
0.451
—
0.0



LNU120
25463.7
0.713
0.036
58.0



LNU120
25463.3
0.645
0.006
42.8



LNU120
25463.6
0.551
0.020
22.0



LNU124
14501.7
0.732
0.000
62.2



LNU124
14501.1
0.634
0.005
40.5



LNU124
14502.7
0.526
0.283
16.4



LNU132
14102.7
0.596
0.007
32.0



LNU132
14102.9
0.546
0.025
21.0



LNU132
14101.9
0.486
0.368
7.7



LNU140
14112.7
0.666
0.098
47.5



LNU140
14111.6
0.565
0.074
25.1



LNU180
24724.3
0.805
0.000
78.4



LNU180
24723.3
0.630
0.212
39.6



LNU180
24721.4
0.508
0.401
12.5



LNU196
25534.1
0.735
0.003
62.7



LNU196
25533.1
0.536
0.064
18.8



LNU196
25533.3
0.513
0.552
13.7



LNU20
24933.2
0.653
0.075
44.8



LNU36
25562.3
0.665
0.001
47.2



LNU36
25562.4
0.540
0.131
19.5



LNU71
25853.4
0.741
0.001
64.1



LNU71
25852.4
0.510
0.187
13.0



CONTROL
—
0.595
—
0.0



LNU1
24681.3
0.709
0.020
19.2



LNU110
24952.3
0.665
0.293
11.8



LNU110
24953.3
0.636
0.554
6.9



LNU175
24733.4
0.921
0.050
54.8



LNU175
24732.2
0.777
0.288
30.7



LNU19
25151.1
0.781
0.024
31.3



LNU215
24663.4
0.833
0.092
40.0



LNU27
24873.1
0.882
0.005
48.4



LNU44
24924.3
0.871
0.059
46.4



LNU54
24903.5
0.780
0.169
31.1



LNU79
24884.4
0.866
0.000
45.6



LNU79
24881.1
0.810
0.007
36.3



LNU79
24884.3
0.696
0.240
17.0



CONTROL
—
0.674
—
0.0



LNU109
24891.2
1.109
0.005
64.4



LNU109
24892.6
0.925
0.007
37.1



LNU109
24892.5
0.848
0.274
25.7



LNU109
24891.5
0.790
0.157
17.2



LNU110
24952.1
1.232
0.008
82.7



LNU110
24954.1
1.043
0.005
54.6



LNU110
24953.2
0.971
0.000
44.0



LNU110
24952.3
0.971
0.064
44.0



LNU110
24954.3
0.816
0.036
21.0



LNU133
24741.2
1.252
0.000
85.6



LNU133
24741.1
1.225
0.000
81.7



LNU133
24744.3
1.178
0.000
74.7



LNU133
24742.2
0.924
0.057
37.0



LNU133
24744.2
0.886
0.033
31.4



LNU19
25151.1
1.249
0.001
85.2



LNU19
25153.3
0.867
0.184
28.5



LNU19
25151.11
0.689
0.740
2.1



LNU27
24873.4
1.248
0.017
85.1



LNU27
24871.4
0.847
0.076
25.5



LNU27
24873.1
0.750
0.098
11.2



LNU44
24922.3
1.096
0.008
62.6



LNU44
24924.3
0.924
0.016
37.0



LNU44
24923.1
0.801
0.040
18.7



LNU54
24903.5
1.320
0.005
95.8



LNU54
24902.4
1.016
0.094
50.6



LNU54
24901.2
0.862
0.242
27.8



LNU6
24994.5
1.197
0.051
77.4



LNU6
24992.3
1.154
0.013
71.0



LNU6
24994.1
0.800
0.326
18.6



LNU6
24994.2
0.772
0.294
14.5



LNU79
24882.2
1.111
0.005
64.8



LNU79
24881.1
1.064
0.014
57.8



LNU79
24884.4
1.023
0.070
51.7



LNU79
24883.2
0.958
0.079
42.1



LNU79
24884.3
0.910
0.051
34.9



CONTROL
—
0.559
—
0.0



LNU109
24892.8
1.054
0.000
88.6



LNU109
24891.5
0.876
0.050
56.8



LNU109
24891.2
0.860
0.022
53.8



LNU143
25975.2
0.731
0.020
30.8



LNU143
25972.1
0.719
0.187
28.7



LNU143
25975.3
0.619
0.264
10.8



LNU154
14604.7
0.890
0.003
59.3



LNU154
14601.6
0.747
0.029
33.6



LNU154
14604.6
0.695
0.143
24.3



LNU154
14602.8
0.661
0.147
18.3



LNU196
25532.2
1.107
0.041
98.0



LNU196
25534.1
0.910
0.002
62.8



LNU196
25531.2
0.644
0.401
15.2



LNU207
24642.5
1.002
0.017
79.2



LNU207
24642.4
0.734
0.031
31.3



LNU207
24644.18
0.653
0.190
16.7



LNU207
24644.13
0.593
0.657
6.0



LNU288
14564.9
0.770
0.011
37.8



LNU288
14562.12
0.740
0.130
32.4



LNU288
14562.7
0.669
0.149
19.7



LNU288
14562.9
0.647
0.157
15.8



LNU288
14562.1
0.629
0.249
12.6



LNU50
26024.2
0.845
0.009
51.2



LNU50
26025.4
0.731
0.051
30.8



LNU50
26023.2
0.598
0.452
7.0



LNU52
25723.2
0.891
0.002
59.4



LNU52
25721.3
0.878
0.031
57.1



LNU52
25721.4
0.735
0.149
31.5



CONTROL
—
0.682
—
0.0



LNU154
14601.6
0.842
0.479
23.4



LNU207
24642.5
1.017
0.003
49.0



LNU207
24642.4
0.762
0.283
11.7



LNU52
25721.4
0.962
0.049
41.1



LNU52
25721.1
0.841
0.089
23.3



LNU52
25723.1
0.796
0.166
16.7



LNU69
14571.1
0.857
0.065
25.6



LNU69
14572.8
0.721
0.699
5.8



CONTROL
—
0.671
—
0.0



LNU150
24843.5
0.871
0.043
29.8



LNU150
24842.9
0.819
0.046
22.1



LNU150
24841.9
0.792
0.226
17.9



LNU150
24843.9
0.703
0.750
4.7



LNU232
26003.7
0.797
0.151
18.7



LNU242
25474.1
0.929
0.011
38.4



LNU242
25473.1
0.807
0.260
20.2



LNU242
25471.1
0.727
0.448
8.3



LNU76
26421.2
0.849
0.257
26.5



LNU76
26422.2
0.709
0.692
5.6



LNU95
13985.15
0.775
0.359
15.4



LNU95
13985.11
0.773
0.220
15.2



CONTROL
—
0.673
—
0.0



LNU118
14013.8
0.865
0.061
28.5



LNU118
14013.6
0.858
0.008
27.3



LNU118
14012.15
0.830
0.176
23.3



LNU118
14012.12
0.755
0.105
12.1



LNU118
14012.14
0.747
0.298
11.0



LNU150
24842.9
1.049
0.004
55.7



LNU150
24841.9
0.886
0.001
31.6



LNU150
24841.6
0.706
0.577
4.8



LNU179
24632.7
0.863
0.063
28.1



LNU179
24631.7
0.822
0.051
22.1



LNU179
24631.6
0.724
0.338
7.5



LNU179
24632.5
0.721
0.567
7.1



LNU232
26001.5
0.850
0.038
26.2



LNU232
26003.3
0.728
0.265
8.1



LNU232
26003.6
0.693
0.747
2.8



LNU235
26184.4
1.120
0.011
66.3



LNU235
26184.2
0.945
0.003
40.3



LNU235
26185.2
0.930
0.006
38.1



LNU235
26182.1
0.700
0.566
3.9



LNU242
25474.1
0.813
0.050
20.7



LNU288
14563.9
1.152
0.001
71.1



LNU288
14562.1
0.970
0.007
44.0



LNU288
14564.9
0.863
0.189
28.2



LNU288
14563.6
0.799
0.109
18.7



LNU288
14562.7
0.768
0.398
14.1



LNU76
26421.2
0.775
0.254
15.0



LNU76
26422.2
0.730
0.443
8.4



LNU76
26425.1
0.723
0.524
7.3



LNU76
26423.1
0.721
0.490
7.1



LNU95
13985.16
1.140
0.004
69.3



LNU95
13985.15
1.040
0.019
54.4



LNU95
13985.12
0.863
0.019
28.2



LNU95
13985.19
0.742
0.339
10.1



CONTROL
—
0.587
—
0.0



LNU101
27635.1
0.700
0.037
19.3



LNU101
27632.1
0.693
0.073
18.0



LNU101
27632.7
0.630
0.160
7.3



LNU128
26515.3
0.812
0.011
38.3



LNU128
26515.2
0.700
0.303
19.3



LNU192
28315.2
0.682
0.177
16.2



LNU192
28313.2
0.609
0.756
3.8



LNU206
27621.1
0.621
0.678
5.8



LNU211
24771.1
0.785
0.066
33.7



LNU282
27563.3
0.811
0.008
38.2



LNU69
14571.1
0.824
0.009
40.3



LNU69
14573.5
0.681
0.042
16.0



LNU69
14572.9
0.629
0.134
7.2



LNU75
27572.1
0.820
0.003
39.6



LNU75
27572.2
0.691
0.164
17.7



CONTROL
—
0.552
—
0.0



LNU101
27632.5
0.745
0.060
34.9



LNU118
14013.6
0.692
0.094
25.3



LNU118
14012.15
0.649
0.267
17.5



LNU118
14013.9
0.570
0.713
3.2



LNU206
27621.2
0.776
0.001
40.5



LNU206
27621.1
0.644
0.120
16.7



LNU249
26153.1
0.883
0.034
59.9



LNU282
27563.1
0.724
0.117
31.1



LNU282
27565.2
0.611
0.277
10.7



LNU288
14563.9
0.866
0.002
57.0



LNU288
14564.8
0.854
0.000
54.7



LNU288
14562.9
0.730
0.072
32.2



LNU288
14562.7
0.643
0.244
16.5



LNU288
14563.6
0.600
0.538
8.7



LNU75
27571.4
0.782
0.049
41.7



LNU75
27572.3
0.743
0.085
34.5



LNU75
27572.2
0.691
0.169
25.1



LNU75
27571.2
0.641
0.112
16.1



CONTROL
—
0.642
—
0.0



LNU11
28204.3
0.685
0.490
6.6



LNU11
28204.1
0.676
0.655
5.2



LNU14
27824.2
0.763
0.613
18.8



LNU183
24863.12
1.132
0.001
76.2



LNU183
24863.1
0.922
0.003
43.6



LNU183
24865.1
0.908
0.018
41.3



LNU183
24864.6
0.736
0.125
14.5



LNU201
28223.1
0.662
0.688
3.1



LNU268
26044.2
0.758
0.210
18.0



LNU268
26045.1
0.719
0.210
11.9



CONTROL
—
0.542
—
0.0



LNU11
28204.1
0.672
0.073
24.1



LNU11
28205.1
0.652
0.233
20.4



LNU11
28205.2
0.652
0.099
20.4



LNU11
28203.2
0.615
0.226
13.6



LNU11
28204.3
0.603
0.316
11.4



LNU112
28212.4
0.688
0.070
27.1



LNU112
28212.1
0.605
0.413
11.8



LNU14
27821.4
0.673
0.156
24.3



LNU14
27821.3
0.671
0.217
24.0



LNU14
27823.2
0.638
0.158
17.8



LNU183
24865.1
1.352
0.000
149.7



LNU183
24863.12
1.265
0.011
133.6



LNU183
24863.1
1.237
0.000
128.4



LNU183
24864.6
1.231
0.000
127.3



LNU183
24864.7
0.586
0.464
8.2



LNU191
28325.4
0.740
0.061
36.6



LNU191
28324.2
0.718
0.046
32.6



LNU191
28325.3
0.664
0.080
22.5



LNU191
28323.1
0.653
0.442
20.6



LNU201
28222.2
0.891
0.036
64.5



LNU201
28223.3
0.616
0.301
13.7



LNU201
28221.3
0.561
0.783
3.6



LNU268
26043.4
0.715
0.025
32.0



LNU268
26041.4
0.685
0.049
26.5



LNU268
26041.6
0.588
0.639
8.6



LNU268
26045.1
0.578
0.753
6.8



CONTROL
—
0.441
—
0.0



LNU107
14585.5
0.576
0.422
30.6



LNU107
14583.8
0.573
0.226
29.9



LNU107
14584.9
0.534
0.315
21.0



LNU116
14494.5
0.616
0.067
39.7



LNU116
14493.6
0.594
0.075
34.7



LNU116
14492.9
0.539
0.011
22.2



LNU116
14492.5
0.530
0.156
20.3



LNU121
25642.2
0.892
0.002
102.2



LNU121
27713.4
0.708
0.004
60.6



LNU121
27713.1
0.689
0.064
56.1



LNU121
27711.1
0.666
0.064
50.9



LNU126
25343.3
0.680
0.000
54.1



LNU126
25345.1
0.656
0.054
48.8



LNU126
25343.1
0.618
0.135
40.0



LNU158
27433.3
0.633
0.045
43.5



LNU158
27433.2
0.556
0.195
26.1



LNU158
27432.5
0.520
0.523
17.8



LNU177
24762.6
0.626
0.100
41.9



LNU177
24765.2
0.531
0.253
20.4



LNU177
24764.9
0.500
0.348
13.4



LNU182
25384.5
0.581
0.034
31.8



LNU182
27521.4
0.553
0.394
25.3



LNU182
25384.1
0.529
0.310
20.0



LNU182
25384.2
0.497
0.219
12.7



LNU2
25713.1
0.618
0.057
40.0



LNU2
27842.1
0.519
0.628
17.6



LNU2
27842.3
0.497
0.464
12.7



LNU225
25991.5
0.547
0.210
24.0



LNU225
25991.2
0.487
0.532
10.3



LNU239
26284.1
0.652
0.019
47.7



LNU239
26283.2
0.527
0.219
19.4



LNU239
26281.1
0.472
0.550
6.9



LNU57
27854.3
0.491
0.236
11.3



LNU83
27684.1
0.490
0.592
11.0



LNU83
27681.4
0.472
0.584
7.1



CONTROL
—
0.297
—
0.0



LNU107
14584.9
0.620
0.002
108.6



LNU107
14585.2
0.527
0.063
77.1



LNU107
14585.5
0.435
0.088
46.4



LNU107
14583.8
0.370
0.175
24.3



LNU107
14583.1
0.344
0.429
15.7



LNU116
14492.5
0.627
0.035
110.8



LNU116
14493.6
0.482
0.085
62.1



LNU116
14491.5
0.470
0.086
57.9



LNU116
14494.5
0.401
0.051
34.8



LNU116
14492.9
0.365
0.320
22.8



LNU121
27713.4
0.901
0.001
202.9



LNU121
27713.1
0.656
0.008
120.7



LNU121
27713.3
0.544
0.002
83.0



LNU121
25642.2
0.534
0.012
79.7



LNU121
27711.1
0.494
0.080
66.1



LNU126
25343.1
0.564
0.001
89.8



LNU126
25343.3
0.435
0.286
46.3



LNU126
25343.4
0.366
0.124
23.2



LNU126
25345.1
0.365
0.150
22.7



LNU158
27433.3
0.707
0.011
137.7



LNU158
27432.5
0.696
0.000
134.0



LNU158
27433.2
0.584
0.020
96.3



LNU158
27434.5
0.338
0.249
13.7



LNU158
27434.1
0.310
0.798
4.3



LNU177
24762.6
0.434
0.130
46.1



LNU177
24763.6
0.352
0.452
18.5



LNU177
24764.12
0.348
0.287
17.1



LNU182
25384.1
0.515
0.026
73.2



LNU182
25384.6
0.510
0.161
71.7



LNU182
25384.2
0.451
0.001
51.6



LNU182
25384.5
0.353
0.448
18.7



LNU182
27521.4
0.328
0.531
10.3



LNU2
27845.3
0.556
0.109
87.0



LNU2
25713.1
0.522
0.023
75.5



LNU2
27842.3
0.503
0.001
69.3



LNU2
27842.1
0.453
0.019
52.4



LNU2
27845.2
0.352
0.351
18.4



LNU225
25991.2
0.762
0.000
156.2



LNU225
25991.3
0.582
0.016
95.9



LNU225
25991.1
0.473
0.147
59.1



LNU225
25991.8
0.472
0.067
58.9



LNU225
25991.5
0.331
0.343
11.2



LNU239
26284.2
0.503
0.042
69.2



LNU239
26283.2
0.491
0.014
65.2



LNU239
26281.1
0.475
0.029
59.7



LNU239
26284.1
0.430
0.145
44.6



LNU239
26283.3
0.422
0.161
41.9



LNU57
27852.1
0.508
0.006
71.0



LNU57
27851.2
0.472
0.001
58.6



LNU57
27854.5
0.330
0.420
11.1



LNU83
27685.1
0.554
0.099
86.4



LNU83
27685.2
0.510
0.000
71.7



LNU83
27681.4
0.399
0.286
34.3



LNU83
27682.1
0.368
0.168
24.0



LNU83
27684.1
0.336
0.653
12.9



CONTROL
—
0.467

0.0



LNU129
27501.2
0.636
<0.1
36.3



LNU129
27502.4
0.527
<0.4
13.0



LNU129
27504.2
0.687
<0.1
47.1



LNU129
27504.3
0.897
<0.1
92.2



LNU147
27513.2
0.654
<0.1
40.2



LNU147
27514.1
0.540
<0.4
15.6



LNU147
27514.2
0.536
<0.4
14.8



LNU153
24851.3
0.490
<0.7
5.0



LNU189
26382.3
0.626
<0.1
34.0



LNU189
26382.4
0.597
<0.1
28.0



LNU189
26383.1
0.666
<0.1
42.6



LNU189
26385.1
0.524
<0.4
12.3



LNU189
26385.2
0.513
<0.7
9.9



LNU219
27461.1
0.784
<0.1
67.9



LNU219
27462.1
0.773
<0.1
65.6



LNU219
27462.2
0.644
<0.1
38.0



LNU219
27464.1
0.499
<0.7
7.0



LNU256
26212.3
0.650
<0.1
39.3



LNU256
26213.1
0.630
<0.1
34.9



LNU256
26214.1
0.649
<0.1
39.0



LNU257
26254.1
0.522
<0.4
11.9



LNU257
26254.3
0.883
<0.1
89.1



LNU257
26254.7
0.589
<0.2
26.1



LNU257
26255.3
0.589
<0.2
26.1



LNU261
27403.2
0.646
<0.1
38.3



LNU261
27405.1
0.646
<0.1
38.5



LNU33
25552.2
0.679
<0.1
45.4



LNU33
25553.2
0.592
<0.2
26.9



LNU33
25555.1
0.503
<0.7
7.9



LNU35
27421.2
0.574
<0.2
22.9



LNU35
27422.1
0.903
<0.1
93.4



LNU35
27423.3
0.615
<0.1
31.8



LNU35
27424.3
0.584
<0.2
25.1



LNU35
27424.4
0.607
<0.1
30.1



LNU50
26022.1
0.725
<0.1
55.3



LNU50
26023.2
0.604
<0.1
29.4



LNU50
26023.3
0.557
<0.2
19.2



LNU50
26024.1
0.730
<0.1
56.3



LNU50
26025.3
0.859
<0.1
84.0



LNU70
25313.1
0.735
<0.1
57.5



LNU70
25313.2
0.657
<0.1
40.8







Table 71.













TABLE 72







Genes showing improved plant performance at standard


nitrogen growth conditions (T1 generation)











Plant Biomass

Plant Biomass



Fresh Weight

Dry Weight



[gr.]

[gr.]














Gene


%
Gene


%


Name
Ave.
p-value
incr.
Name
Ave.
p-value
incr.

















CONT.
0.160
—
0.0
CONT.
0.008
—
0.0


LNU121
0.176
0.305
9.8
LNU121
0.009
0.243
19.5


LNU150
0.160
0.987
0.2
LNU154
0.008
0.489
11.3


LNU154
0.166
0.808
4.0
CONT.
0.005
—
0.0


CONT.
0.118
—
0.0
LNU275
0.010
0.005
85.2


LNU275
0.189
0.022
60.3
LNU57
0.006
0.574
10.3


LNU57
0.124
0.697
5.1
LNU64
0.006
0.254
14.1


LNU64
0.127
0.509
7.6
LNU83
0.006
0.295
18.9


LNU83
0.132
0.385
12.1
CONT.
0.005
—
0.0


CONT.
0.110
—
0.0
LNU59
0.005
0.560
12.0


LNU176
0.115
0.670
4.5
LNU60
0.006
0.553
12.5


LNU247
0.112
0.884
1.6






LNU284
0.112
0.941
1.5






CONT.
0.128
—
0.0






LNU59
0.134
0.717
5.0






LNU60
0.135
0.703
5.5





Table 72.


“CONT.”—Control;


“Ave.”—Average;


“% Incr.” = % increment.













TABLE 73







Genes showing improved plant performance at standard nitrogen


growth conditions (T1 generation)









Leaf Area [cm2]











Gene Name
Time Point
Average
p-value
% increment














CONTROL
Leaf_Area_TP2
0.328
—
0.0


LNU154
Leaf_Area_TP2
0.334
0.886
1.6


CONTROL
Leaf_Area_TP3
0.629
—
0.0


LNU121
Leaf_Area_TP3
0.649
0.667
3.1


CONTROL
Leaf_Area_TP2
0.289
—
0.0


LNU127
Leaf_Area_TP2
0.295
0.783
2.3


LNU275
Leaf_Area_TP2
0.492
0.000
70.5


LNU32
Leaf_Area_TP2
0.309
0.519
7.1


LNU57
Leaf_Area_TP2
0.313
0.309
8.4


LNU58
Leaf_Area_TP2
0.305
0.421
5.8


LNU83
Leaf_Area_TP2
0.381
0.032
32.1


CONTROL
Leaf_Area_TP3
0.626
—
0.0


LNU275
Leaf_Area_TP3
0.981
0.004
56.7


LNU57
Leaf_Area_TP3
0.691
0.354
10.3


LNU64
Leaf_Area_TP3
0.630
0.935
0.7


LNU83
Leaf_Area_TP3
0.781
0.069
24.7


CONTROL
Leaf_Area_TP2
0.298
—
0.0


LNU59
Leaf_Area_TP2
0.302
0.885
1.4


CONTROL
Leaf_Area_TP3
0.606
—
0.0


LNU59
Leaf_Area_TP3
0.631
0.619
4.1





Table 73.






The genes listed in Tables 74 and 75 improved plant NUE when grown at standard nitrogen concentration levels. These genes produced larger root biomass (root length and root coverage) when grown under standard nitrogen growth conditions, compared to control plants. Plants producing larger root biomass have better possibilities to absorb larger amount of nitrogen from soil. The genes were cloned under the regulation of a constitutive promoter (At6669) or root preferred promoter (RootP). The evaluation of each gene was performed by testing the performance of different number of events. Some of the genes were evaluated in more than one tissue culture assay resulting in positive results as well. Event with p-value <0.1 was considered statistically significant







TABLE 74







Genes showing improved root performance at standard nitrogen growth conditions


(T2 generation)











Roots Length [cm]

Roots Coverage [cm2]
















Gene


p-
%
Gene


p-
%


Name
Event #
Ave.
value
incr.
Name
Event #
Ave.
value
incr.



















CONT.
—
4.849
—
0.0
CONT.
—
3.976
—
0.0


LNU100
14474.3
6.391
0.001
31.8
LNU100
14474.3
5.804
0.076
46.0


LNU100
14471.4
6.031
0.073
24.4
LNU100
14474.4
5.605
0.215
41.0


LNU100
14474.4
5.910
0.082
21.9
LNU100
14471.4
5.036
0.150
26.7


LNU100
14473.1
5.412
0.078
11.6
LNU104
25033.3
5.092
0.128
28.1


LNU100
14473.3
5.137
0.473
5.9
LNU213
24654.4
6.150
0.072
54.7


LNU104
25033.3
6.109
0.003
26.0
LNU213
24653.2
5.740
0.034
44.4


LNU104
25032.1
5.255
0.247
8.4
LNU213
24651.1
4.940
0.159
24.3


LNU104
25033.1
5.022
0.613
3.6
LNU218
24783.2
7.745
0.049
94.8


LNU104
25032.2
4.979
0.747
2.7
LNU218
24781.2
4.894
0.179
23.1


LNU213
24653.2
6.170
0.004
27.2
LNU218
24781.7
4.617
0.254
16.1


LNU213
24654.4
6.029
0.014
24.3
LNU218
24781.4
4.145
0.714
4.3


LNU213
24651.1
5.610
0.111
15.7
LNU4
25134.1
4.657
0.201
17.1


LNU213
24653.1
5.144
0.485
6.1
LNU48
24802.2
4.922
0.256
23.8


LNU218
24783.2
7.188
0.000
48.2
LNU48
24804.4
4.630
0.443
16.5


LNU218
24781.1
5.595
0.039
15.4
LNU48
24803.2
4.430
0.384
11.4


LNU218
24781.7
5.435
0.155
12.1
LNU8
25063.1
7.354
0.004
85.0


LNU218
24781.4
5.290
0.210
9.1
LNU8
25062.2
5.008
0.068
26.0


LNU218
24781.2
5.196
0.253
7.2
LNU8
25063.6
4.121
0.730
3.7


LNU4
25131.1
6.021
0.008
24.2
LNU94
24833.1
4.236
0.661
6.5


LNU4
25134.1
5.680
0.098
17.1
LNU94
24834.4
4.130
0.709
3.9


LNU4
25134.2
5.462
0.041
12.6
CONT.
—
5.584
—
0.0


LNU4
25133.3
5.018
0.587
3.5
LNU1
24682.2
7.395
0.107
32.4


LNU48
24804.4
5.956
0.010
22.8
LNU1
24682.1
6.727
0.071
20.5


LNU48
24802.2
5.586
0.106
15.2
LNU1
24684.1
6.661
0.177
19.3


LNU48
24803.2
5.049
0.544
4.1
LNU1
24681.1
6.270
0.284
12.3


LNU8
25063.1
6.763
0.000
39.5
LNU133
24744.3
9.891
0.000
77.1


LNU8
25062.2
6.296
0.001
29.8
LNU133
24741.1
9.192
0.066
64.6


LNU8
25063.6
5.123
0.416
5.7
LNU133
24741.2
6.714
0.087
20.2


LNU8
25062.1
5.016
0.610
3.5
LNU175
24732.1
9.540
0.038
70.8


LNU94
24834.4
5.557
0.058
14.6
LNU175
24732.4
9.071
0.011
62.5


LNU94
24833.3
5.351
0.233
10.4
LNU175
24734.4
8.342
0.004
49.4


CONT.
—
6.645
—
0.0
LNU175
24731.2
5.955
0.657
6.6


LNU1
24682.2
6.883
0.409
3.6
LNU178
14614.5
8.913
0.010
59.6


LNU1
24684.1
6.833
0.350
2.8
LNU178
14611.5
7.578
0.133
35.7


LNU133
24744.3
6.958
0.328
4.7
LNU178
14612.1
6.454
0.303
15.6


LNU175
24732.1
7.028
0.152
5.8
LNU178
14611.4
5.776
0.777
3.4


LNU175
24732.4
6.739
0.677
1.4
LNU215
24664.3
9.619
0.027
72.3


LNU178
14614.5
7.100
0.133
6.8
LNU215
24661.4
7.432
0.016
33.1


LNU215
24664.2
6.776
0.643
2.0
LNU215
24663.4
6.228
0.402
11.5


LNU215
24664.3
6.739
0.765
1.4
LNU215
24664.2
5.760
0.756
3.2


CONT.
—
5.816
—
0.0
LNU24
24971.4
8.367
0.080
49.8


LNU120
25463.7
7.237
0.001
24.4
LNU24
24973.1
7.707
0.151
38.0


LNU120
25463.6
6.531
0.009
12.3
LNU6
24992.3
9.043
0.105
61.9


LNU120
25463.3
6.304
0.096
8.4
LNU6
24993.3
7.325
0.282
31.2


LNU124
14502.1
6.609
0.003
13.6
LNU82
24823.1
7.225
0.154
29.4


LNU124
14502.7
6.545
0.113
12.5
LNU82
24824.3
5.882
0.638
5.4


LNU124
14501.1
6.332
0.094
8.9
LNU9
25001.3
8.110
0.025
45.2


LNU124
14501.7
5.964
0.685
2.5
LNU9
25001.1
7.174
0.035
28.5


LNU132
14102.9
6.457
0.057
11.0
LNU9
25001.2
6.256
0.317
12.0


LNU132
14102.7
6.113
0.611
5.1
CONT.
—
4.531
—
0.0


LNU140
14114.8
6.579
0.061
13.1
LNU120
25463.7
7.868
0.038
73.6


LNU140
14111.6
6.576
0.004
13.1
LNU120
25463.6
5.970
0.030
31.8


LNU140
14112.7
5.926
0.577
1.9
LNU120
25463.3
5.164
0.181
14.0


LNU180
24723.3
6.266
0.142
7.7
LNU124
14502.7
5.914
0.076
30.5


LNU180
24724.3
6.232
0.321
7.1
LNU124
14501.7
5.595
0.192
23.5


LNU180
24724.1
6.204
0.096
6.7
LNU124
14502.1
5.257
0.081
16.0


LNU196
25534.1
5.957
0.581
2.4
LNU122
14501.1
5.079
0.170
12.1


LNU196
25533.3
5.955
0.740
2.4
LNU132
14102.7
5.508
0.146
21.6


LNU20
24933.2
6.550
0.079
12.6
LNU132
14102.9
5.228
0.247
15.4


LNU20
24933.4
6.103
0.334
4.9
LNU140
14111.6
5.840
0.083
28.9


LNU36
25562.3
7.052
0.001
21.2
LNU140
14112.7
5.539
0.146
22.2


LNU36
25562.4
6.098
0.448
4.9
LNU140
14114.8
5.460
0.025
20.5


LNU71
25852.5
5.985
0.623
2.9
LNU180
24724.3
6.600
0.013
45.7


CONT.
—
5.715
—
0.0
LNU180
241233
5.650
0.177
24.7


LNU1
24684.1
5.994
0.624
4.9
LNU180
24721.4
4.990
0.306
10.1


LNU1
24682.1
5.985
0.700
4.7
LNU180
24724.1
4.848
0.394
7.0


LNU110
24953.2
6.472
0.204
13.2
LNU196
25534.1
5.798
0.172
28.0


LNU110
24954.3
6.240
0.371
9.2
LNU196
25533.3
4.974
0.596
9.8


LNU110
24953.3
5.887
0.761
3.0
LNU196
25533.1
4.907
0.537
8.3


LNU175
24733.4
7.017
0.056
22.8
LNU20
24933.2
6.361
0.076
40.4


LNU175
24732.1
6.599
0.155
15.5
LNU20
24932.4
5.171
0.277
14.1


LNU175
24734.4
6.338
0.297
10.9
LNU20
24933.4
4.880
0.392
7.7


LNU19
25151.11
5.917
0.721
3.5
LNU36
25562.3
7.451
0.014
64.4


LNU215
24664.2
6.822
0.089
19.4
LNU36
25562.4
5.452
0.174
20.3


LNU215
24663.3
6.562
0.164
14.8
LNU71
25853.4
6.607
0.024
45.8


LNU215
24663.4
6.429
0.239
12.5
CONT.
—
5.186
—
0.0


LNU215
24661.4
6.327
0.285
10.7
LNU1
24681.3
6.080
0.219
17.2


LNU27
24873.1
6.861
0.096
20.0
LNU110
24952.3
5.702
0.579
10.0


LNU27
24871.4
6.399
0.243
12.0
LNU175
24733.4
9.339
0.011
80.1


LNU27
24873.4
5.879
0.794
2.9
LNU175
24732.2
6.572
0.148
26.7


LNU44
24923.3
6.618
0.168
15.8
LNU175
24734.4
5.788
0.436
11.6


LNU44
24924.2
6.437
0.234
12.6
LNU175
24732.1
5.692
0.490
9.8


LNU44
24922.3
6.341
0.322
11.0
LNU19
25151.1
5.793
0.383
11.7


LNU44
24923.1
5.999
0.607
5.0
LNU215
24663.4
7.608
0.035
46.7


LNU54
24901.2
7.145
0.047
25.0
LNU215
24664.2
6.954
0.131
34.1


LNU54
24902.4
7.103
0.062
24.3
LNU215
24663.3
5.781
0.411
11.5


LNU54
24902.7
6.053
0.543
5.9
LNU215
24663.1
5.562
0.795
7.3


LNU54
24903.3
5.999
0.668
5.0
LNU27
24873.1
8.055
0.022
55.3


LNU54
24903.5
5.976
0.679
4.6
LNU27
24873.4
5.906
0.364
13.9


LNU79
24881.1
7.091
0.049
24.1
LNU27
24871.4
5.882
0.322
13.4


LNU79
24882.2
6.898
0.082
20.7
LNU44
24924.2
6.515
0.098
25.6


LNU79
24883.2
6.280
0.322
9.9
LNU44
24924.3
6.346
0.246
22.4


LNU79
24884.4
6.016
0.595
5.3
LNU44
24923.3
6.219
0.267
19.9


CONT.
—
6.033
—
0.0
LNU44
24922.3
5.394
0.779
4.0


LNU109
24892.6
6.662
0.003
10.4
LNU54
24902.4
7.593
0.092
46.4


LNU109
24892.8
6.584
0.089
9.1
LNU54
24901.2
7.095
0.160
36.8


LNU109
24892.5
6.471
0.091
7.3
LNU54
24903.5
6.720
0.141
29.6


LNU109
24891.5
6.398
0.149
6.0
LNU54
24903.3
6.233
0.284
20.2


LNU109
24891.2
6.168
0.624
2.2
LNU79
24881.1
9.394
0.001
81.1


LNU110
24952.1
7.116
0.032
17.9
LNU79
24884.4
6.787
0.061
30.9


LNU110
24952.3
6.584
0.252
9.1
LNU79
24884.3
5.863
0.407
13.1


LNU110
24954.1
6.477
0.388
7.4
LNU79
24882.2
5.824
0.552
12.3


LNU110
24953.2
6.476
0.414
7.3
CONT.
—
5.925
—
0.0


LNU110
24954.3
6.318
0.252
4.7
LNU109
24892.6
9.651
0.001
62.9


LNU133
24744.3
7.193
0.003
19.2
LNU109
24892.5
8.357
0.129
41.1


LNU133
24741.2
6.655
0.043
10.3
LNU109
24891.2
7.582
0.064
28.0


LNU133
24741.1
6.592
0.059
9.3
LNU109
24891.5
7.440
0.090
25.6


LNU133
24742.2
6.248
0.133
3.6
LNU109
24892.8
6.366
0.511
7.5


LNU19
25151.1
6.386
0.125
5.9
LNU110
24952.1
10.431
0.010
76.1


LNU27
24873.1
7.085
0.070
17.4
LNU110
24954.1
9.168
0.016
54.7


LNU27
24871.4
7.029
0.003
16.5
LNU110
24953.2
8.763
0.119
47.9


LNU27
24873.4
6.424
0.115
6.5
LNU110
24954.3
8.415
0.020
42.0


LNU27
24872.3
6.125
0.692
1.5
LNU110
24952.3
8.298
0.094
40.1


LNU44
24922.3
6.695
0.003
11.0
LNU133
24744.3
9.969
0.000
68.3


LNU44
24923.3
6.472
0.305
7.3
LNU133
24741.2
9.419
0.006
59.0


LNU44
24924.3
6.113
0.701
1.3
LNU133
24741.1
9.029
0.014
52.4


LNU54
24901.2
6.844
0.049
13.4
LNU133
24742.2
8.023
0.041
35.4


LNU54
24903.5
6.790
0.005
12.5
LNU133
24744.2
7.532
0.080
27.1


LNU54
24902.4
6.782
0.066
12.4
LNU19
25151.1
9.075
0.003
53.2


LNU54
24903.3
6.624
0.045
9.8
LNU19
25153.3
7.527
0.201
27.0


LNU54
24902.7
6.298
0.398
4.4
LNU19
25151.11
7.438
0.043
25.6


LNU6
24994.2
6.782
0.062
12.4
LNU27
24873.4
8.650
0.067
46.0


LNU6
24992.3
6.759
0.044
12.0
LNU27
24871.4
8.011
0.092
35.2


LNU6
24994.5
6.673
0.102
10.6
LNU27
24873.1
7.767
0.045
31.1


LNU6
24993.3
6.261
0.154
3.8
LNU44
24922.3
10.244
0.000
72.9


LNU79
24884.4
6.952
0.014
15.2
LNU44
24923.3
8.210
0.130
38.6


LNU79
24881.1
6.550
0.233
8.6
LNU44
24924.3
6.559
0.435
10.7


LNU79
24882.2
6.508
0.140
7.9
LNU44
24923.1
6.379
0.492
7.7


LNU79
24883.2
6.422
0.119
6.4
LNU54
24903.5
9.523
0.016
60.7


CONT.
—
6.265
—
0.0
LNU54
24902.4
7.707
0.237
30.1


LNU109
24892.8
6.456
0.444
3.1
LNU54
24901.2
7.417
0.368
25.2


LNU143
25975.3
6.422
0.445
2.5
LNU54
24903.3
6.587
0.292
11.2


LNU154
14601.6
6.582
0.290
5.1
LNU6
24992.3
9.573
0.029
61.6


LNU196
25534.1
6.487
0.491
3.5
LNU6
24994.5
9.480
0.098
60.0


LNU207
24642.5
6.853
0.030
9.4
LNU6
24994.2
8.627
0.028
45.6


LNU207
24641.1
6.636
0.131
5.9
LNU6
24994.1
6.376
0.617
7.6


LNU50
26023.2
6.670
0.171
6.5
LNU79
24884.4
9.253
0.084
56.2


LNU50
26024.2
6.465
0.315
3.2
LNU79
24881.1
8.970
0.067
51.4


LNU52
25723.2
6.373
0.746
1.7
LNU79
24883.2
8.450
0.010
42.6


CONT.
—
5.225
—
0.0
LNU79
24884.3
8.381
0.068
41.5


LNU143
25971.2
6.542
0.010
25.2
LNU79
24882.2
8.338
0.007
40.7


LNU143
25975.3
6.302
0.007
20.6
CONT.
—
5.283
—
0.0


LNU143
25975.2
5.975
0.034
14.4
LNU109
24892.8
7.899
0.001
49.5


LNU143
25971.5
5.900
0.059
12.9
LNU109
24891.5
7.630
0.001
44.4


LNU143
25972.1
5.684
0.234
8.8
LNU143
25975.2
6.615
0.035
25.2


LNU154
14601.6
6.510
0.005
24.6
LNU143
25972.1
5.812
0.490
10.0


LNU207
24641.1
6.531
0.170
25.0
LNU143
25975.3
5.802
0.294
9.8


LNU207
24642.5
6.154
0.029
17.8
LNU154
14604.7
7.402
0.014
40.1


LNU207
24642.4
5.651
0.193
8.2
LNU154
14601.6
6.345
0.203
20.1


LNU211
24771.1
6.217
0.015
19.0
LNU196
25532.2
7.364
0.294
39.4


LNU211
24771.3
5.368
0.648
2.7
LNU196
25534.1
6.774
0.009
28.2


LNU52
25723.1
6.492
0.004
24.3
LNU207
24642.5
7.232
0.151
36.9


LNU52
25721.2
5.400
0.761
3.4
LNU207
24642.4
6.473
0.126
22.5


LNU52
25721.1
5.342
0.673
2.3
LNU207
24644.18
6.146
0.444
16.3


LNU69
14571.1
6.159
0.119
17.9
LNU207
24644.13
5.800
0.264
9.8


LNU69
14573.3
5.824
0.070
11.5
LNU207
24641.1
5.667
0.688
7.3


LNU69
14572.8
5.559
0.427
6.4
LNU288
14562.9
6.574
0.223
24.4


CONT.
—
4.818
—
0.0
LNU288
14564.9
6.230
0.216
17.9


LNU150
24842.9
6.440
0.000
33.7
LNU288
14562.7
5.939
0.495
12.4


LNU150
24843.5
6.075
0.018
26.1
LNU288
14562.12
5.738
0.647
8.6


LNU150
24841.9
5.578
0.117
15.8
LNU50
26025.4
7.278
0.006
37.8


LNU150
24842.5
5.570
0.008
15.6
LNU50
26024.2
7.156
0.012
35.5


LNU179
24631.9
6.537
0.001
35.7
LNU50
26023.5
6.065
0.332
14.8


LNU179
24632.5
6.033
0.024
25.2
LNU50
26023.2
5.464
0.644
3.4


LNU179
24631.6
5.461
0.031
13.4
LNU52
25723.2
7.247
0.018
37.2


LNU179
24632.7
5.320
0.170
10.4
LNU52
25721.4
6.053
0.380
14.6


LNU179
24631.7
5.063
0.520
5.1
LNU52
25721.3
6.011
0.283
13.8


LNU232
26003.3
6.508
0.000
35.1
CONT.
—
6.028
—
0.0


LNU232
26003.7
5.627
0.034
16.8
LNU143
25975.3
6.851
0.315
13.6


LNU232
26001.5
5.375
0.159
11.6
LNU143
25975.2
6.827
0.345
13.3


LNU232
26001.2
5.282
0.078
9.6
LNU154
14601.6
8.039
0.364
33.4


LNU232
26003.6
5.071
0.682
5.3
LNU207
24641.1
7.283
0.464
20.8


LNU235
26184.4
6.139
0.000
27.4
LNU207
24642.5
7.096
0.217
17.7


LNU235
26185.3
5.962
0.005
23.7
LNU207
24642.4
6.809
0.368
13.0


LNU235
26182.1
5.822
0.006
20.9
LNU211
24771.1
7.170
0.287
18.9


LNU235
26184.2
5.461
0.120
13.3
LNU52
25723.1
7.431
0.307
23.3


LNU242
25474.1
6.568
0.008
36.3
LNU69
14571.1
7.692
0.211
27.6


LNU242
25473.1
6.326
0.000
31.3
LNU69
14573.3
6.458
0.686
7.1


LNU242
25473.3
6.163
0.003
27.9
LNU69
14572.8
6.374
0.697
5.7


LNU242
25471.1
6.004
0.000
24.6
CONT.
—
4.102
—
0.0


LNU242
25472.1
5.035
0.595
4.5
LNU150
24843.5
7.038
0.000
71.6


LNU76
26421.2
6.376
0.020
32.3
LNU150
24842.9
7.011
0.010
70.9


LNU76
26421.1
5.555
0.007
15.3
LNU150
24841.9
6.414
0.003
56.4


LNU76
26422.2
5.466
0.117
13.4
LNU150
24843.9
5.198
0.098
26.7


LNU76
26425.1
5.383
0.280
11.7
LNU179
24631.9
5.977
0.047
45.7


LNU76
26423.1
5.317
0.252
10.4
LNU179
24632.5
5.099
0.209
24.3


LNU95
13985.11
6.468
0.007
34.3
LNU232
26003.7
6.928
0.092
68.9


LNU95
13985.16
5.968
0.001
23.9
LNU232
26003.3
6.034
0.029
47.1


LNU95
13985.19
5.886
0.023
22.2
LNU232
26003.6
4.465
0.691
8.8


LNU95
13985.15
5.821
0.033
20.8
LNU232
26001.5
4.325
0.792
5.4


LNU95
13985.12
5.454
0.255
13.2
LNU235
26185.3
5.570
0.023
35.8


CONT.
—
5.596
—
0.0
LNU235
26184.4
5.454
0.110
33.0


LNU150
24842.9
6.495
0.077
16.1
LNU235
26182.1
4.710
0.157
14.8


LNU150
24843.5
5.728
0.782
2.4
LNU235
26184.2
4.681
0.569
14.1


LNU179
24631.7
6.000
0.346
7.2
LNU242
25474.1
7.610
0.042
85.5


LNU179
24631.6
5.967
0.337
6.6
LNU242
25473.1
5.905
0.000
44.0


LNU179
24632.5
5.884
0.329
5.1
LNU242
25471.1
5.858
0.000
42.8


LNU232
26001.5
6.960
0.001
24.4
LNU242
25473.3
4.707
0.315
14.8


LNU232
26003.3
6.334
0.055
13.2
LNU242
25472.1
4.342
0.761
5.8


LNU235
26184.4
6.801
0.001
21.5
LNU76
26421.2
7.302
0.025
78.0


LNU235
26185.2
6.719
0.002
20.1
LNU76
26421.1
5.648
0.015
37.7


LNU235
26184.2
6.703
0.030
19.8
LNU76
26423.1
4.653
0.574
13.4


LNU235
26182.1
6.005
0.414
7.3
LNU76
26422.2
4.641
0.416
13.1


LNU242
25474.1
6.141
0.238
9.7
LNU76
26425.1
4.577
0.624
11.6


LNU288
14563.9
6.445
0.009
15.2
LNU95
13985.11
7.498
0.065
82.8


LNU288
14562.1
6.389
0.118
14.2
LNU95
13985.15
6.783
0.001
65.4


LNU288
14563.6
6.158
0.246
10.1
LNU95
13985.16
5.850
0.119
42.6


LNU288
14562.7
5.913
0.492
5.7
LNU95
13985.19
5.634
0.228
37.3


LNU288
14564.9
5.793
0.539
3.5
LNU95
13985.12
4.641
0.605
13.1


LNU76
26421.2
5.827
0.492
4.1
CONT.
—
5.171
—
0.0


LNU95
13985.11
6.313
0.049
12.8
LNU118
14013.6
6.735
0.106
30.3


LNU95
13985.12
6.257
0.025
11.8
LNU118
14012.15
6.697
0.326
29.5


LNU95
13985.16
6.116
0.328
9.3
LNU150
24842.9
7.643
0.119
47.8


LNU95
13985.15
6.092
0.238
8.9
LNU150
24841.9
6.335
0.177
22.5


LNU95
13985.19
5.758
0.625
2.9
LNU150
24843.5
6.217
0.117
20.2


CONT.
—
5.840
—
0.0
LNU150
24841.6
5.370
0.709
3.8


LNU101
27632.5
6.847
0.035
17.2
LNU179
24632.7
6.009
0.211
16.2


LNU101
27635.1
6.709
0.082
14.9
LNU179
24631.7
5.926
0.488
14.6


LNU101
27632.7
6.522
0.040
11.7
LNU232
26001.5
8.215
0.001
58.9


LNU101
27632.1
6.490
0.176
11.1
LNU232
26003.3
7.174
0.037
38.7


LNU101
27632.6
6.066
0.662
3.9
LNU235
26184.4
10.268
0.005
98.6


LNU128
26511.4
6.453
0.186
10.5
LNU235
26185.2
7.934
0.016
53.4


LNU128
26515.3
6.427
0.238
10.1
LNU235
26184.2
7.761
0.020
50.1


LNU128
26515.2
6.181
0.362
5.8
LNU235
26182.1
5.904
0.305
14.2


LNU128
26511.5
6.170
0.399
5.7
LNU242
25474.1
7.459
0.024
44.2


LNU192
28313.3
6.947
0.003
19.0
LNU288
14563.9
8.852
0.004
71.2


LNU192
28315.2
6.906
0.021
18.3
LNU288
14562.1
7.472
0.019
44.5


LNU192
28313.2
6.630
0.147
13.5
LNU288
14563.6
6.435
0.217
24.4


LNU206
27621.2
6.699
0.296
14.7
LNU288
14564.9
5.887
0.167
13.9


LNU206
27622.1
5.925
0.681
1.5
LNU288
14562.7
5.436
0.775
5.1


LNU282
27563.3
6.365
0.161
9.0
LNU76
26421.2
5.861
0.250
13.3


LNU69
14573.5
6.062
0.437
3.8
LNU76
26425.1
5.685
0.341
9.9


LNU69
14571.1
5.886
0.769
0.8
LNU76
26422.2
5.561
0.685
7.5


LNU75
27572.1
6.841
0.023
17.2
LNU76
26423.1
5.357
0.778
3.6


LNU75
27572.2
6.360
0.207
8.9
LNU95
13985.15
7.642
0.003
47.8


LNU75
27571.4
6.307
0.312
8.0
LNU95
13985.16
7.507
0.080
45.2


CONT.
—
5.421
—
0.0
LNU95
13985.12
6.609
0.092
27.8


LNU101
27635.1
6.330
0.001
16.8
CONT.
—
5.155
—
0.0


LNU101
27632.5
6.284
0.042
15.9
LNU101
27632.1
6.954
0.000
34.9


LNU118
14013.9
5.551
0.582
2.4
LNU101
27635.1
6.553
0.015
27.1


LNU128
26515.3
6.567
0.004
21.2
LNU101
27632.5
6.041
0.341
17.2


LNU206
27621.2
7.079
0.000
30.6
LNU101
27632.6
5.659
0.660
9.8


LNU206
27622.4
6.005
0.027
10.8
LNU128
26515.3
8.036
0.036
55.9


LNU206
27622.1
5.675
0.201
4.7
LNU128
26515.2
6.980
0.059
35.4


LNU249
26152.4
6.731
0.047
24.2
LNU128
26511.4
6.416
0.275
24.5


LNU249
26152.2
6.097
0.021
12.5
LNU192
28315.2
7.992
0.006
55.0


LNU249
26151.1
5.809
0.442
7.2
LNU192
28313.2
7.389
0.056
43.3


LNU249
26153.1
5.805
0.330
7.1
LNU192
28313.3
6.309
0.015
22.4


LNU282
27565.2
6.755
0.000
24.6
LNU206
27621.2
7.023
0.099
36.2


LNU282
27562.1
6.371
0.181
17.5
LNU206
27621.1
6.013
0.362
16.6


LNU282
27563.3
6.358
0.001
17.3
LNU211
24771.1
6.969
0.137
35.2


LNU282
27563.1
5.930
0.327
9.4
LNU282
27563.3
6.654
0.242
29.1


LNU282
27565.1
5.878
0.248
8.4
LNU69
14571.1
6.648
0.029
29.0


LNU288
14563.6
6.686
0.019
23.3
LNU69
14573.5
6.575
0.082
27.5


LNU288
14564.8
5.991
0.025
10.5
LNU75
27572.2
7.816
0.048
51.6


LNU288
14562.9
5.894
0.051
8.7
LNU75
27572.3
6.896
0.047
33.8


LNU288
14563.9
5.770
0.351
6.4
LNU75
27572.1
6.642
0.116
28.8


LNU288
14562.7
5.498
0.730
1.4
LNU75
27571.4
5.830
0.472
13.1


LNU75
27571.4
6.877
0.013
26.9
CONT.
—
5.119
—
0.0


LNU75
27571.2
6.627
0.007
22.3
LNU101
27632.5
7.394
0.082
44.4


LNU75
27572.2
6.434
0.000
18.7
LNU101
27635.1
5.507
0.659
7.6


LNU75
27572.1
6.343
0.001
17.0
LNU118
14012.15
6.364
0.142
24.3


LNU75
27572.3
6.135
0.197
13.2
LNU118
14013.6
6.134
0.192
19.8


CONT.
—
5.851
—
0.0
LNU118
14013.9
5.512
0.470
7.7


LNU11
28203.2
6.770
0.084
15.7
LNU206
27621.2
7.528
0.002
47.1


LNU11
28202.5
6.655
0.030
13.7
LNU249
26153.1
6.400
0.084
25.0


LNU11
28205.2
6.256
0.263
6.9
LNU249
26152.4
5.917
0.429
15.6


LNU11
28204.1
5.953
0.773
1.7
LNU282
27563.1
6.191
0.310
20.9


LNU112
28212.1
7.103
0.025
21.4
LNU282
27565.2
5.531
0.547
8.0


LNU112
28212.4
6.658
0.025
13.8
LNU288
14564.8
7.100
0.026
38.7


LNU14
27823.2
6.785
0.007
16.0
LNU288
14563.6
6.894
0.136
34.7


LNU14
27821.1
6.697
0.015
14.5
LNU288
14562.9
6.758
0.062
32.0


LNU14
27821.3
6.536
0.084
11.7
LNU288
14563.9
6.601
0.128
28.9


LNU183
24864.6
6.449
0.296
10.2
LNU288
14562.7
5.378
0.745
5.1


LNU201
28223.1
6.657
0.018
13.8
LNU75
27572.2
7.467
0.086
45.9


LNU201
28222.3
6.486
0.069
10.9
LNU75
27571.4
7.273
0.099
42.1


LNU201
28223.3
6.296
0.220
7.6
LNU75
27571.2
6.832
0.023
33.5


LNU201
28222.2
6.038
0.526
3.2
LNU75
27572.3
6.252
0.407
22.1


LNU268
26044.2
6.891
0.055
17.8
CONT.
—
6.888
—
0.0


LNU268
26041.6
6.017
0.556
2.8
LNU14
27821.3
7.893
0.445
14.6


CONT.
—
6.335
—
0.0
LNU14
27823.2
7.231
0.669
5.0


LNU11
28205.2
7.266
0.038
14.7
LNU183
24863.12
7.193
0.724
4.4


LNU11
28203.2
6.971
0.204
10.0
LNU268
26044.2
8.591
0.288
24.7


LNU11
28204.3
6.549
0.598
3.4
CONT.
—
6.290
—
0.0


LNU112
28212.4
6.616
0.460
4.4
LNU11
28205.2
9.031
0.002
43.6


LNU112
28212.1
6.561
0.530
3.6
LNU11
28203.2
8.193
0.057
30.3


LNU14
27821.3
7.170
0.048
13.2
LNU11
28205.1
7.731
0.091
22.9


LNU183
24863.1
6.996
0.242
10.4
LNU11
28204.3
7.658
0.100
21.8


LNU183
24864.6
6.598
0.474
4.2
LNU11
28202.5
7.517
0.152
19.5


LNU191
28323.1
6.529
0.614
3.1
LNU11
28204.1
6.773
0.246
7.7


LNU201
28222.2
6.989
0.131
10.3
LNU112
28212.4
7.304
0.058
16.1


LNU268
26043.4
6.545
0.603
3.3
LNU112
28212.1
6.984
0.195
11.0


CONT.
—
5.852
—
0.0
LNU14
27821.3
8.988
0.064
42.9


LNU107
14585.5
6.287
0.288
7.4
LNU14
27823.2
7.003
0.606
11.3


LNU116
14492.5
6.136
0.610
4.8
LNU183
24865.1
11.077
0.001
76.1


LNU121
27711.1
6.388
0.223
9.2
LNU183
24863.1
10.729
0.057
70.6


LNU121
27713.4
6.358
0.207
8.7
LNU183
24863.12
8.917
0.023
41.8


LNU126
25343.3
6.722
0.055
14.9
LNU183
24864.6
8.916
0.003
41.8


LNU126
25345.1
6.584
0.135
12.5
LNU191
28323.1
8.175
0.253
30.0


LNU126
25343.1
6.437
0.194
10.0
LNU191
28325.4
6.688
0.356
6.3


LNU126
25343.4
6.144
0.489
5.0
LNU201
28222.2
10.400
0.029
65.4


LNU158
27433.3
7.014
0.109
19.9
LNU201
28223.1
6.822
0.376
8.5


LNU158
27433.2
6.299
0.374
7.6
LNU268
26043.4
8.266
0.057
31.4


LNU158
27432.5
6.176
0.436
5.5
LNU268
26041.6
7.535
0.443
19.8


LNU158
27434.5
6.080
0.686
3.9
LNU268
26041.4
7.476
0.159
18.9


LNU177
24765.2
7.112
0.025
21.5
LNU268
26044.2
6.808
0.370
8.2


LNU177
24762.6
6.607
0.070
12.9
CONT.
—
5.249
—
0.0


LNU177
24764.9
6.268
0.392
7.1
LNU107
14585.5
6.577
0.434
25.3


LNU177
24763.6
6.176
0.396
5.5
LNU107
14583.8
6.445
0.293
22.8


LNU182
27521.4
6.800
0.042
16.2
LNU107
14584.9
5.987
0.175
14.1


LNU182
25384.5
6.767
0.101
15.6
LNU116
14492.5
5.750
0.366
9.5


LNU182
25384.1
6.019
0.762
2.8
LNU116
14494.5
5.574
0.589
6.2


LNU2
27842.3
6.959
0.029
18.9
LNU116
14492.9
5.515
0.583
5.1


LNU2
25713.1
6.193
0.445
5.8
LNU121
27713.4
8.247
0.002
57.1


LNU225
25991.5
7.007
0.038
19.7
LNU121
25642.2
7.499
0.133
42.9


LNU225
25991.2
6.717
0.114
14.8
LNU121
27711.1
7.285
0.022
38.8


LNU225
25991.3
6.613
0.172
13.0
LNU121
27713.1
5.912
0.261
12.6


LNU225
25991.1
6.286
0.464
7.4
LNU126
25345.1
7.763
0.024
47.9


LNU239
26284.1
6.856
0.025
17.2
LNU126
25343.3
6.965
0.016
32.7


LNU239
26284.2
6.651
0.068
13.7
LNU126
25343.1
6.521
0.210
24.2


LNU239
26281.1
6.264
0.439
7.0
LNU126
25343.4
5.499
0.674
4.8


LNU239
26283.3
6.028
0.712
3.0
LNU158
27433.3
8.968
0.137
70.8


LNU57
27852.1
6.999
0.020
19.6
LNU158
27433.2
8.046
0.004
53.3


LNU57
27854.3
6.744
0.059
15.2
LNU158
27432.5
6.290
0.360
19.8


LNU83
27684.1
6.527
0.133
11.5
LNU177
24762.6
8.887
0.085
69.3


LNU83
27681.4
6.381
0.260
9.0
LNU177
24765.2
7.192
0.269
37.0


LNU83
27682.1
6.213
0.357
6.2
LNU177
24764.9
5.932
0.179
13.0


LNU83
27685.1
5.973
0.771
2.1
LNU182
25384.5
7.997
0.058
52.3


CONT.
—
5.715
—
0.0
LNU182
27521.4
7.490
0.053
42.7


LNU107
14584.9
6.891
0.021
20.6
LNU182
25384.1
6.840
0.039
30.3


LNU107
14583.8
6.667
0.061
16.7
LNU2
27842.3
7.641
0.069
45.6


LNU107
14585.5
6.158
0.237
7.8
LNU2
25713.1
7.344
0.011
39.9


LNU116
14491.5
6.504
0.094
13.8
LNU2
27842.1
6.809
0.436
29.7


LNU116
14494.5
5.784
0.774
1.2
LNU225
25991.5
9.233
0.029
75.9


LNU121
27713.1
7.058
0.001
23.5
LNU225
25991.2
7.038
0.116
34.1


LNU121
27711.1
6.792
0.005
18.9
LNU225
25991.1
6.470
0.244
23.3


LNU121
27713.4
6.571
0.098
15.0
LNU225
25991.3
6.141
0.551
17.0


LNU126
25343.1
7.209
0.000
26.2
LNU239
26284.1
6.751
0.113
28.6


LNU126
25343.4
6.524
0.086
14.2
LNU239
26283.2
5.920
0.548
12.8


LNU126
25345.1
6.324
0.216
10.7
LNU239
26281.1
5.794
0.397
10.4


LNU126
25343.3
6.246
0.108
9.3
LNU57
27852.1
7.854
0.013
49.6


LNU158
27433.3
7.495
0.000
31.2
LNU57
27854.3
6.344
0.102
20.9


LNU158
27432.5
7.179
0.000
25.6
LNU57
27854.5
5.880
0.366
12.0


LNU158
27433.2
7.006
0.002
22.6
LNU83
27681.4
7.249
0.097
38.1


LNU158
27434.1
6.795
0.000
18.9
LNU83
27684.1
5.767
0.589
9.9


LNU158
27434.5
6.698
0.055
17.2
LNU83
27685.1
5.561
0.651
5.9


LNU177
24764.12
7.403
0.006
29.6
CONT.
—
4.915
—
0.0


LNU177
24763.6
7.161
0.001
25.3
LNU107
14584.9
10.490
0.008
113.4


LNU177
24765.2
7.070
0.000
23.7
LNU107
14583.8
7.949
0.019
61.7


LNU177
24764.9
5.894
0.552
3.1
LNU107
14585.2
6.870
0.009
39.8


LNU182
25384.2
6.882
0.008
20.4
LNU107
14583.1
5.412
0.458
10.1


LNU182
25384.6
6.781
0.012
18.7
LNU107
14585.5
5.284
0.413
7.5


LNU182
27521.4
6.773
0.017
18.5
LNU116
14493.6
7.433
0.046
51.2


LNU182
25384.1
6.597
0.012
15.5
LNU116
14494.5
7.121
0.098
44.9


LNU182
25384.5
6.430
0.002
12.5
LNU116
14491.5
7.065
0.145
43.8


LNU2
27842.1
7.238
0.000
26.7
LNU116
14492.5
6.075
0.007
23.6


LNU2
25713.1
6.894
0.001
20.6
LNU116
14492.9
5.198
0.791
5.8


LNU2
27842.3
6.701
0.035
17.3
LNU121
27713.4
10.520
0.009
114.1


LNU225
25991.3
7.743
0.000
35.5
LNU121
27713.1
9.336
0.011
90.0


LNU225
25991.5
7.166
0.001
25.4
LNU121
27711.1
7.107
0.029
44.6


LNU225
25991.2
6.660
0.008
16.6
LNU121
25642.2
5.748
0.472
17.0


LNU225
25991.8
6.386
0.139
11.7
LNU126
25343.1
7.086
0.055
44.2


LNU225
25991.1
6.180
0.020
8.1
LNU126
25343.3
5.673
0.262
15.4


LNU239
26284.1
6.838
0.005
19.7
LNU126
25343.4
5.597
0.015
13.9


LNU239
26281.1
6.800
0.002
19.0
LNU126
25345.1
5.517
0.332
12.3


LNU239
26283.3
6.798
0.002
19.0
LNU158
27433.3
10.395
0.000
111.5


LNU239
26284.2
6.544
0.042
14.5
LNU158
27432.5
10.059
0.009
104.7


LNU239
26283.2
6.142
0.233
7.5
LNU158
27433.2
7.322
0.044
49.0


LNU57
27852.1
7.280
0.003
27.4
LNU158
27434.5
7.124
0.031
44.9


LNU57
27854.5
7.061
0.000
23.6
LNU158
27434.1
6.038
0.135
22.9


LNU57
27851.2
7.054
0.000
23.4
LNU177
24764.12
7.964
0.000
62.1


LNU57
27854.3
6.356
0.004
11.2
LNU177
24763.6
6.811
0.057
38.6


LNU83
27685.1
7.354
0.000
28.7
LNU177
24765.2
5.371
0.457
9.3


LNU83
27682.1
7.128
0.034
24.7
LNU182
25384.6
8.604
0.031
75.1


LNU83
27685.2
7.081
0.000
23.9
LNU182
25384.1
7.588
0.003
54.4


LNU83
27681.4
6.754
0.002
18.2
LNU182
25384.2
7.046
0.020
43.4


LNU83
27684.1
6.555
0.118
14.7
LNU182
27521.4
6.026
0.156
22.6







LNU182
25384.5
5.953
0.156
21.1







LNU2
27842.1
8.180
0.000
66.4







LNU2
27842.3
7.899
0.005
60.7







LNU2
25713.1
7.574
0.090
54.1







LNU2
27845.2
5.617
0.386
14.3







LNU225
25991.2
11.048
0.002
124.8







LNU225
25991.3
10.621
0.024
116.1







LNU225
25991.8
7.226
0.094
47.0







LNU225
25991.5
6.962
0.001
41.7







LNU225
25991.1
6.869
0.003
39.8







LNU239
26283.3
7.477
0.057
52.1







LNU239
26281.1
7.013
0.013
42.7







LNU239
26284.2
5.944
0.170
21.0







LNU239
26284.1
5.651
0.207
15.0







LNU239
26283.2
5.605
0.113
14.0







LNU57
27854.5
9.187
0.002
86.9







LNU57
27851.2
9.142
0.003
86.0







LNU57
27852.1
8.944
0.006
82.0







LNU57
27854.3
5.731
0.095
16.6







LNU83
27685.1
8.992
0.003
83.0







LNU83
27685.2
8.420
0.046
71.3







LNU83
27682.1
7.321
0.092
49.0







LNU83
27681.4
6.918
0.004
40.8







LNU83
27684.1
5.685
0.383
15.7







CONT.
—
7.469
—








LNU17
13991.1
8.395
0.2
12.3







CONT.
—
5.518

0.0







LNU129
27501.2
7.071
<0.1
28.2







LNU129
27502.4
6.038
<0.8
9.4







LNU129
27503.4
5.784
<0.8
4.8







LNU129
27504.3
9.078
<0.05
64.5







LNU147
27513.2
7.281
<0.1
32.0







LNU147
27514.2
6.115
<0.8
10.8







LNU153
24851.3
7.253
<0.1
31.4







LNU189
26382.4
6.849
<0.2
24.1







LNU189
26383.1
6.937
<0.2
25.7







LNU189
26385.1
6.744
<0.2
22.2







LNU219
27462.1
8.526
<0.1
54.5







LNU219
27464.2
7.111
<0.1
28.9







LNU256
26212.3
5.987
<0.8
8.5







LNU256
26214.1
8.193
<0.05
48.5







LNU256
26214.2
6.910
<0.2
25.2







LNU257
26254.1
6.804
<0.2
23.3







LNU257
26254.3
9.461
<0.05
71.5







LNU257
26255.3
6.481
<0.5
17.5







LNU261
27403.2
5.664
<0.9
2.7







LNU33
25552.2
7.217
<0.1
30.8







LNU33
25555.1
5.970
<0.8
8.2







LNU35
27422.1
7.462
<0.1
35.2







LNU35
27424.3
6.419

16.3







LNU35
27424.4
7.739
<0.05
40.2







LNU50
26022.1
7.402
<0.05
34.1







LNU50
26024.1
7.051
<0.1
27.8







LNU50
26025.3
8.616
<0.05
56.1







LNU70
25311.3
5.862
<0.8
6.2







LNU70
25311.4
6.021
<0.8
9.1







LNU70
25313.1
6.324
<0.7
14.6





Table 74.


“CONT.”—Control;


“Ave”—Average;


“% Incr.” = % increment.













TABLE 75







Genes showing improved root performance at standard nitrogen growth conditions


(T1 generation)













Roots Coverage



Roots Length [cm]

[cm2]
















Gene
Time

p-
%
Gene


p-
%


Name
Point
Ave.
value
incr.
Name
Time Point
Ave.
value
incr.



















CONT.
Roots
1.840
—
0.0
CONT.
Roots
0.925
—
0.0



Length




Coverage






TP2




TP2





LNU107
Roots
1.922
0.619
4.4
LNU118
Roots
0.951
0.651
2.9



Length




Coverage






TP2




TP2





LNU181
Roots
1.856
0.869
0.9
LNU121
Roots
1.581
0.007
70.9



Length




Coverage






TP2




TP2





LNU121
Roots
2.431
0.015
32.1
LNU150
Roots
0.939
0.839
1.6



Length




Coverage






TP2




TP2





LNU154
Roots
2.237
0.176
21.5
LNU154
Roots
1.227
0.250
32.7



Length




Coverage






TP2




TP2





LNU210
Roots
2.124
0.122
15.4
LNU210
Roots
1.180
0.032
27.7



Length




Coverage






TP2




TP2





LNU68
Roots
1.915
0.646
4.1
LNU68
Roots
0.931
0.961
0.6



Length




Coverage






TP2




TP2





CONT.
Roots_Length_TP3
3.468
—
0.0
CONT.
Roots
2.488
—
0.0








Coverage











TP3





LNU121
Roots_Length_TP3
4.286
0.007
23.6
LNU121
Roots
4.048
0.000
62.7








Coverage











TP3





LNU154
Roots_Length_TP3
3.653
0.613
5.4
LNU154
Roots
2.785
0.583
12.0








Coverage











TP3





LNU210
Roots_Length_TP3
3.707
0.366
6.9
LNU210
Roots
2.770
0.188
11.3








Coverage











TP3





CONT.
Roots
1.572
—
0.0
CONT.
Roots
1.015
—
0.0



Length




Coverage






TP2




TP2





LNU127
Roots
1.797
0.327
14.3
LNU265
Roots
1.029
0.935
1.3



Length




Coverage






TP2




TP2





LNU188
Roots
1.769
0.191
12.5
LNU275
Roots
2.413
0.003
137.6



Length




Coverage






TP2




TP2





LNU2
Roots
1.673
0.574
6.4
LNU57
Roots
1.571
0.008
54.7



Length




Coverage






TP2




TP2





LNU239
Roots
1.895
0.214
20.5
LNU58
Roots
1.301
0.143
28.1



Length




Coverage






TP2




TP2





LNU258
Roots
1.648
0.689
4.8
LNU83
Roots
1.659
0.013
63.3



Length




Coverage






TP2




TP2





LNU265
Roots
1.947
0.030
23.8
CONT.
Roots
2.621
—
0.0



Length




Coverage






TP2




TP3





LNU275
Roots
2.607
0.002
65.8
LNU275
Roots
7.009
0.005
167.5



Length




Coverage






TP2




TP3





LNU32
Roots
1.886
0.107
20.0
LNU57
Roots
4.363
0.004
66.5



Length




Coverage






TP2




TP3





LNU57
Roots
2.332
0.000
48.3
LNU58
Roots
3.190
0.066
21.7



Length




Coverage






TP2




TP3





LNU58
Roots
2.138
0.001
36.0
LNU83
Roots
4.487
0.007
71.2



Length




Coverage






TP2




TP3





LNU64
Roots
1.642
0.633
4.4
CONT.
Roots
0.613
—
0.0



Length




Coverage






TP2




TP2





LNU83
Roots
2.568
0.001
63.3
LNU176
Roots
0.663
0.738
8.2



Length




Coverage






TP2




TP2





CONT.
Roots_Length_TP3
3.387
—
0.0
LNU214
Roots
0.845
0.016
38.0








Coverage











TP2





LNU239
Roots_Length_TP3
3.480
0.842
2.7
LNU223
Roots
0.741
0.189
20.9








Coverage











TP2





LNU265
Roots_Length_TP3
3.489
0.675
3.0
LNU233
Roots
0.764
0.159
24.7








Coverage











TP2





LNU275
Roots_Length_TP3
4.574
0.017
35.0
LNU245
Roots
0.794
0.027
29.6








Coverage











TP2





LNU57
Roots_Length_TP3
4.598
0.002
35.7
LNU247
Roots
0.673
0.653
9.9








Coverage











TP2





LNU58
Roots_Length_TP3
3.745
0.059
10.5
LNU251
Roots
0.645
0.748
5.2








Coverage











TP2





LNU83
Roots_Length_TP3
4.733
0.003
39.7
LNU284
Roots
0.710
0.330
15.8








Coverage











TP2





CONT.
Roots
1.563
—
0.0
LNU289
Roots
0.880
0.014
43.6



Length




Coverage






TP2




TP2





LNU176
Roots
1.695
0.430
8.4
LNU70
Roots
0.902
0.041
47.1



Length




Coverage






TP2




TP2





LNU214
Roots
2.127
0.006
36.1
LNU85
Roots
0.852
0.012
39.0



Length




Coverage






TP2




TP2





LNU223
Roots
1.907
0.005
22.0
LNU86
Roots
0.665
0.618
8.5



Length




Coverage






TP2




TP2





LNU233
Roots
1.932
0.003
23.6
CONT.
Roots
1.504
—
0.0



Length




Coverage






TP2




TP3





LNU245
Roots
2.021
0.001
29.3
LNU176
Roots
2.038
0.118
35.5



Length




Coverage






TP2




TP3





LNU247
Roots
1.690
0.621
8.1
LNU214
Roots
1.816
0.190
20.7



Length




Coverage






TP2




TP3





LNU251
Roots
1.944
0.251
24.4
LNU223
Roots
1.925
0.066
27.9



Length




Coverage






TP2




TP3





LNU284
Roots
1.925
0.198
23.2
LNU233
Roots
1.884
0.253
25.2



Length




Coverage






TP2




TP3





LNU289
Roots
2.082
0.003
33.2
LNU245
Roots
1.644
0.332
9.3



Length




Coverage






TP2




TP3





LNU70
Roots
1.921
0.157
22.9
LNU247
Roots
1.527
0.933
1.5



Length




Coverage






TP2




TP3





LNU85
Roots
2.118
0.007
35.5
LNU284
Roots
1.802
0.345
19.8



Length




Coverage






TP2




TP3





LNU86
Roots
1.690
0.435
8.1
LNU289
Roots
1.991
0.011
32.4



Length




Coverage






TP2




TP3





CONT.
Roots_Length_TP3
2.862
—
0.0
LNU70
Roots
2.020
0.140
34.2








Coverage











TP3





LNU176
Roots_Length_TP3
3.292
0.150
15.0
LNU85
Roots
2.097
0.066
39.4








Coverage











TP3





LNU214
Roots_Length_TP3
3.448
0.062
20.5
LNU86
Roots
1.521
0.955
1.1








Coverage











TP3





LNU223
Roots_Length_TP3
3.310
0.050
15.6
CONT.
Roots
1.338
—
0.0








Coverage











TP2





LNU233
Roots_Length_TP3
3.361
0.073
17.5
LNU105
Roots
1.395
0.693
4.3








Coverage











TP2





LNU245
Roots_Length_TP3
3.407
0.043
19.0
LNU115
Roots
1.405
0.617
5.0








Coverage











TP2





LNU247
Roots_Length_TP3
2.993
0.659
4.6
LNU134
Roots
1.689
0.236
26.2








Coverage











TP2





LNU251
Roots_Length_TP3
2.986
0.779
4.3
LNU190
Roots
1.556
0.392
16.3








Coverage











TP2





LNU284
Roots_Length_TP3
3.321
0.267
16.0
LNU198
Roots
1.410
0.715
5.4








Coverage











TP2





LNU289
Roots_Length_TP3
3.375
0.016
17.9
LNU200
Roots
1.753
0.054
31.0








Coverage











TP2





LNU70
Roots_Length_TP3
3.398
0.192
18.7
CONT.
Roots
3.172
—
0.0








Coverage











TP3





LNU85
Roots_Length_TP3
3.726
0.032
30.2
LNU105
Roots
3.383
0.707
6.7








Coverage











TP3





CONT.
Roots
2.410
—
0.0
LNU115
Roots
3.577
0.321
12.8



Length




Coverage






TP2




TP3





LNU105
Roots
2.686
0.077
11.5
LNU134
Roots
3.704
0.399
16.8



Length




Coverage






TP2




TP3





LNU115
Roots
2.523
0.420
4.7
LNU190
Roots
3.202
0.958
1.0



Length




Coverage






TP2




TP3





LNU123
Roots
2.532
0.386
5.1
LNU198
Roots
3.239
0.885
2.1



Length




Coverage






TP2




TP3





LNU134
Roots
2.759
0.093
14.4
LNU200
Roots
3.387
0.625
6.8



Length




Coverage






TP2




TP3





LNU190
Roots
2.613
0.533
8.4
CONT.
Roots
1.312
—
0.0



Length




Coverage






TP2




TP2





LNU198
Roots
2.559
0.606
6.2
LNU51
Roots
1.395
0.554
6.4



Length




Coverage






TP2




TP2





LNU200
Roots
2.803
0.129
16.3
LNU59
Roots
1.312
0.999
0.0



Length




Coverage






TP2




TP2





CONT.
Roots_Length_TP3
3.919
—
0.0
CONT.
Roots
3.140
—
0.0








Coverage











TP3





LNU105
Roots_Length_TP3
4.351
0.235
11.0
LNU51
Roots
3.256
0.671
3.7








Coverage











TP3





LNU115
Roots_Length_TP3
4.242
0.218
8.3
LNU59
Roots
3.253
0.612
3.6








Coverage











TP3





LNU134
Roots_Length_TP3
4.380
0.178
11.8







LNU190
Roots_Length_TP3
3.931
0.978
0.3







LNU198
Roots_Length_TP3
4.030
0.799
2.9







LNU200
Roots_Length_TP3
4.038
0.734
3.0







CONT.
Roots
2.210
—
0.0








Length











TP2










LNU225
Roots
2.248
0.809
1.7








Length











TP2










LNU243
Roots
2.283
0.627
3.3








Length











TP2










LNU244
Roots
2.266
0.846
2.6








Length











TP2










LNU51
Roots
2.712
0.022
22.7








Length











TP2










LNU59
Roots
2.522
0.315
14.1








Length











TP2










LNU60
Roots
2.420
0.377
9.5








Length











TP2










CONT.
Roots_Length_TP3
4.117
—
0.0







LNU225
Roots_Length_TP3
4.183
0.834
1.6







LNU244
Roots_Length_TP3
4.133
0.973
0.4







LNU51
Roots_Length_TP3
4.561
0.105
10.8







LNU59
Roots_Length_TP3
4.405
0.210
7.0







LNU60
Roots_Length_TP3
4.125
0.984
0.2





Table 75.


“CONT.”—Control;


“Ave.”—Average;


“% Incr.” = % increment.






The genes listed in Tables 76 and 77 improved plant growth rate (leaf area, root length and root coverage growth rate) when grown at standard nitrogen concentration levels. These produced plants that grew faster than control plants when grown under standard nitrogen growth conditions. Faster growth was observed when growth rate of leaf area and root length and coverage was measured. The genes were cloned under the regulation of a constitutive promoter (At6669) or root preferred promoter (RootP). Evaluation of each gene was performed by testing the performance of different number of events. Some of the genes were evaluated in more than one tissue culture assay resulting in positive results as well. Event with p-value <0.1 was considered statistically significant







TABLE 76







Genes showing improved growth rate at standard nitrogen growth conditions (T2 generation)













RGR Of Leaf

RGR Of Roots

RGR Of Roots



Area

Coverage

Length





















Gene


p-
%
Gene


p-
%
Gene


p-
%


Name
Event #
Ave.
value
incr.
Name
Event #
Ave.
value
incr.
Name
Event #
Ave.
value
incr.
























CONT.
—
0.05
—
0
CONT.
—
0.47
—
0
CONT.
—
0.42
—
0


LNU100
14474.3
0.09
0.02
72
LNU100
14474.3
0.67
0.03
42
LNU100
14471.4
0.49
0.34
16


LNU100
14474.4
0.06
0.20
29
LNU100
14474.4
0.66
0.08
42
LNU100
14474.4
0.48
0.29
13


LNU100
14471.4
0.05
0.50
10
LNU100
14471.4
0.60
0.09
29
LNU100
14474.3
0.47
0.19
11


LNU104
25033.3
0.07
0.03
51
LNU104
25033.3
0.60
0.09
28
LNU100
14473.1
0.46
0.26
10


LNU213
24654.4
0.09
0.01
81
LNU213
24654.4
0.73
0.01
56
LNU100
14473.3
0.44
0.72
4


LNU213
24653.2
0.09
0.00
79
LNU213
24653.2
0.66
0.01
41
LNU104
25033.3
0.49
0.07
17


LNU213
24651.1
0.06
0.04
31
LNU213
24651.1
0.58
0.11
24
LNU213
24654.4
0.49
0.14
17


LNU218
24783.2
0.11
0.00
121
LNU218
24783.2
0.93
0.00
98
LNU213
24653.2
0.48
0.14
15


LNU218
24781.7
0.06
0.07
31
LNU218
24781.2
0.57
0.16
22
LNU213
24651.1
0.45
0.61
6


LNU4
25134.1
0.07
0.01
41
LNU218
24781.7
0.55
0.20
18
LNU218
24783.2
0.65
0.00
54


LNU4
25134.2
0.05
0.51
10
LNU4
25134.1
0.55
0.18
19
LNU218
24781.2
0.45
0.35
8


LNU48
24803.2
0.08
0.01
53
LNU48
24802.2
0.58
0.15
25
LNU218
24781.4
0.45
0.46
8


LNU48
24802.2
0.06
0.07
28
LNU48
24804.4
0.54
0.43
15
LNU4
25134.1
0.48
0.28
14


LNU48
24804.4
0.06
0.21
21
LNU48
24803.2
0.53
0.38
12
LNU4
25131.1
0.44
0.67
4


LNU8
25063.1
0.08
0.01
57
LNU8
25063.1
0.87
0.00
87
LNU48
24802.2
0.44
0.65
5


LNU8
25062.2
0.07
0.04
45
LNU8
25062.2
0.58
0.08
24
LNU48
24804.4
0.44
0.64
5


LNU8
25063.6
0.06
0.08
29
LNU94
24833.1
0.49
0.73
5
LNU8
25063.1
0.57
0.00
36


LNU94
24833.3
0.06
0.17
20
CONT.
—
0.64
—
0
LNU8
25062.2
0.49
0.10
17


LNU94
24834.1
0.05
0.78
5
LNU1
24682.2
0.87
0.04
36
LNU94
24834.1
0.43
0.80
3


CONT.
—
0.08
—
0
LNU1
24684.1
0.77
0.13
20
CONT.
—
0.56
—
0


LNU1
24681.3
0.09
0.30
19
LNU1
24682.1
0.77
0.08
20
LNU1
24682.2
0.59
0.70
4


LNU1
24682.2
0.09
0.37
15
LNU1
24681.1
0.71
0.36
11
LNU178
14614.5
0.59
0.51
5


LNU133
24744.3
0.13
0.00
70
LNU133
24744.3
1.13
0.00
77
LNU215
24661.4
0.58
0.79
3


LNU133
24741.1
0.13
0.01
69
LNU133
24741.1
1.06
0.02
65
CONT.
—
0.53
—
0


LNU175
24732.1
0.11
0.03
42
LNU133
24741.2
0.74
0.16
16
LNU120
25463.7
0.71
0.00
34


LNU175
24734.4
0.11
0.03
42
LNU175
24732.1
1.10
0.00
71
LNU120
25463.6
0.58
0.18
11


LNU175
24732.4
0.09
0.47
12
LNU175
24732.4
1.05
0.00
64
LNU120
25463.3
0.58
0.23
10


LNU175
24731.2
0.08
0.66
7
LNU175
24734.4
0.98
0.00
53
LNU120
25464.1
0.55
0.68
4


LNU178
14614.5
0.10
0.09
29
LNU175
24731.2
0.70
0.52
10
LNU124
14502.7
0.59
0.17
12


LNU178
14611.5
0.09
0.10
19
LNU178
14614.5
1.05
0.00
63
LNU124
14501.1
0.57
0.33
8


LNU178
14611.1
0.08
0.77
3
LNU178
14611.5
0.89
0.05
40
LNU124
14502.1
0.55
0.53
5


LNU215
24664.3
0.13
0.00
69
LNU178
14612.1
0.74
0.31
15
LNU124
14501.7
0.55
0.62
4


LNU215
24661.4
0.09
0.32
12
LNU178
14611.4
0.67
0.73
4
LNU132
14102.9
0.58
0.19
11


LNU24
24973.1
0.10
0.03
36
LNU215
24664.3
1.12
0.00
74
LNU132
14102.7
0.54
0.78
3


LNU24
24971.4
0.09
0.08
21
LNU215
24661.4
0.88
0.00
38
LNU140
14111.6
0.57
0.22
9


LNU24
24971.2
0.08
0.39
10
LNU215
24663.4
0.70
0.47
10
LNU140
14114.8
0.57
0.36
8


LNU6
24992.3
0.14
0.02
85
LNU215
24664.2
0.67
0.73
4
LNU140
14112.7
0.55
0.62
4


LNU6
24993.3
0.09
0.46
15
LNU24
24971.4
0.98
0.02
53
LNU180
24723.3
0.60
0.10
14


LNU82
24823.1
0.11
0.21
37
LNU24
24973.1
0.90
0.07
40
LNU180
24724.3
0.59
0.22
11


LNU9
25001.3
0.11
0.00
45
LNU6
24992.3
1.04
0.03
63
LNU196
25533.3
0.55
0.65
4


CONT.
—
0.04
—
0
LNU6
24993.3
0.78
0.38
22
LNU20
24933.2
0.59
0.20
11


LNU120
25463.7
0.08
0.00
74
LNU82
24823.1
0.84
0.10
32
LNU20
24933.4
0.55
0.52
5


LNU120
25463.3
0.06
0.00
39
LNU82
24824.3
0.68
0.59
7
LNU36
25562.3
0.62
0.04
17


LNU120
25463.6
0.06
0.01
34
LNU9
25001.3
0.96
0.00
49
LNU71
25853.4
0.54
0.78
2


LNU124
14501.7
0.07
0.00
69
LNU9
25001.1
0.83
0.03
29
CONT.
—
0.51
—
0


LNU124
14501.1
0.06
0.01
35
LNU9
25001.2
0.71
0.37
11
LNU1
24682.1
0.56
0.52
10


LNU124
14502.7
0.05
0.10
25
CONT.
—
0.54
—
0
LNU1
24683.2
0.56
0.54
10


LNU124
14502.1
0.05
0.58
6
LNU120
25463.7
0.96
0.00
77
LNU1
24681.1
0.55
0.64
7


LNU132
14102.7
0.06
0.00
40
LNU120
25463.6
0.72
0.02
33
LNU1
24681.3
0.54
0.70
6


LNU132
14102.9
0.06
0.02
29
LNU120
25463.3
0.62
0.22
14
LNU110
24953.2
0.63
0.12
24


LNU132
14101.9
0.05
0.62
6
LNU124
14502.7
0.72
0.02
32
LNU110
24954.3
0.58
0.34
13


LNU140
14112.7
0.07
0.01
51
LNU124
14501.7
0.68
0.09
25
LNU175
24733.4
0.61
0.19
19


LNU140
14111.6
0.06
0.05
27
LNU124
14502.1
0.62
0.20
15
LNU175
24732.1
0.60
0.20
18


LNU140
14114.8
0.05
0.38
10
LNU124
14501.1
0.60
0.40
11
LNU175
24734.4
0.58
0.36
13


LNU180
24724.3
0.08
0.00
87
LNU132
14102.7
0.66
0.11
22
LNU175
24733.1
0.58
0.40
13


LNU180
24723.3
0.06
0.05
45
LNU132
14102.9
0.63
0.21
16
LNU175
24732.2
0.54
0.65
6


LNU180
24721.4
0.05
0.18
20
LNU140
14111.6
0.70
0.04
29
LNU19
25151.11
0.54
0.71
5


LNU196
25534.1
0.07
0.00
66
LNU140
14112.7
0.67
0.10
23
LNU215
24664.2
0.65
0.07
27


LNU196
25533.1
0.05
0.06
24
LNU140
14114.8
0.65
0.09
21
LNU215
24663.4
0.59
0.31
15


LNU196
25533.3
0.05
0.22
23
LNU180
24724.3
0.80
0.00
47
LNU215
24663.3
0.59
0.29
15


LNU20
24933.2
0.07
0.01
52
LNU180
24723.3
0.69
0.06
27
LNU215
24661.4
0.57
0.39
12


LNU20
24932.4
0.05
0.73
4
LNU180
24721.4
0.59
0.42
10
LNU27
24873.1
0.60
0.23
18


LNU36
25562.3
0.07
0.00
50
LNU180
24724.1
0.58
0.58
6
LNU27
24871.4
0.57
0.40
12


LNU36
25562.4
0.05
0.10
23
LNU196
25534.1
0.70
0.06
29
LNU27
24873.4
0.57
0.48
11


LNU36
25561.2
0.05
0.48
9
LNU196
25533.3
0.60
0.46
11
LNU44
24922.3
0.62
0.16
21


LNU71
25853.4
0.08
0.00
72
LNU196
25533.1
0.59
0.51
9
LNU44
24924.2
0.61
0.25
18


LNU71
25852.4
0.05
0.07
23
LNU20
24933.2
0.77
0.01
42
LNU44
24923.3
0.58
0.34
14


CONT.
—
0.06
—
0
LNU20
24932.4
0.62
0.25
15
LNU44
24923.1
0.57
0.43
11


LNU1
24681.3
0.07
0.01
24
LNU20
24933.4
0.57
0.66
5
LNU54
24901.2
0.69
0.03
34


LNU110
24952.3
0.06
0.42
8
LNU36
25562.3
0.90
0.00
66
LNU54
24902.4
0.67
0.06
31


LNU110
24953.3
0.06
0.76
4
LNU36
25562.4
0.65
0.15
20
LNU54
24902.7
0.59
0.27
15


LNU175
24733.4
0.09
0.00
53
LNU71
25853.4
0.80
0.00
48
LNU54
24903.3
0.57
0.50
11


LNU175
24732.2
0.08
0.15
37
CONT.
—
0.60
—
0
LNU54
24903.5
0.54
0.74
5


LNU19
25151.1
0.08
0.01
31
LNU1
24681.3
0.74
0.12
23
LNU79
24881.1
0.66
0.05
29


LNU215
24663.4
0.09
0.01
48
LNU1
24683.2
0.63
0.80
5
LNU79
24882.2
0.62
0.14
22


LNU27
24873.1
0.09
0.00
53
LNU110
24952.3
0.67
0.49
11
LNU79
24884.4
0.54
0.67
6


LNU44
24924.3
0.09
0.01
44
LNU110
24953.3
0.62
0.80
4
CONT.
—
0.51
—
0


LNU54
24903.5
0.08
0.07
32
LNU175
24733.4
1.10
0.00
83
LNU109
24892.6
0.58
0.03
14


LNU54
24902.4
0.07
0.43
9
LNU175
24732.2
0.80
0.07
32
LNU109
24892.5
0.56
0.14
10


LNU79
24884.4
0.09
0.00
51
LNU175
24734.4
0.69
0.34
14
LNU109
24891.2
0.54
0.37
6


LNU79
24881.1
0.08
0.00
41
LNU175
24732.1
0.68
0.40
13
LNU109
24892.8
0.53
0.59
4


LNU79
24884.3
0.07
0.09
22
LNU19
25151.1
0.69
0.29
15
LNU109
24891.5
0.52
0.68
2


CONT.
—
0.07
—
0
LNU19
25153.3
0.63
0.73
5
LNU110
24952.3
0.59
0.10
15


LNU109
24891.2
0.11
0.00
71
LNU215
24663.4
0.92
0.01
53
LNU110
24952.1
0.58
0.18
13


LNU109
24892.6
0.10
0.00
50
LNU215
24664.2
0.83
0.05
39
LNU110
24953.2
0.56
0.49
9


LNU109
24892.5
0.09
0.12
33
LNU215
24663.3
0.68
0.39
13
LNU110
24954.1
0.56
0.37
9


LNU109
24891.5
0.08
0.08
23
LNU215
24663.1
0.65
0.71
9
LNU110
24954.3
0.53
0.57
4


LNU110
24952.1
0.13
0.00
88
LNU27
24873.1
0.96
0.00
60
LNU133
24744.3
0.61
0.04
19


LNU110
24954.1
0.11
0.00
67
LNU27
24873.4
0.72
0.27
19
LNU133
24741.2
0.56
0.16
10


LNU110
24952.3
0.10
0.01
52
LNU27
24871.4
0.70
0.27
17
LNU133
24741.1
0.56
0.20
10


LNU110
24953.2
0.10
0.00
51
LNU44
24924.2
0.78
0.05
30
LNU133
24742.2
0.54
0.34
5


LNU110
24954.3
0.09
0.01
36
LNU44
24924.3
0.76
0.14
26
LNU19
25151.11
0.54
0.38
6


LNU133
24741.2
0.13
0.00
93
LNU44
24923.3
0.74
0.21
22
LNU19
25151.1
0.53
0.62
3


LNU133
24741.1
0.13
0.00
89
LNU44
24922.3
0.65
0.61
8
LNU27
24873.1
0.62
0.05
21


LNU133
24744.3
0.12
0.00
81
LNU44
24923.1
0.63
0.78
4
LNU27
24871.4
0.61
0.00
19


LNU133
24742.2
0.10
0.00
54
LNU54
24902.4
0.91
0.02
52
LNU27
24873.4
0.54
0.39
5


LNU133
24744.2
0.10
0.00
47
LNU54
24901.2
0.85
0.06
41
LNU44
24924.3
0.54
0.29
6


LNU19
25151.1
0.13
0.00
89
LNU54
24903.5
0.80
0.06
34
LNU44
24923.3
0.54
0.64
5


LNU19
25153.3
0.09
0.06
39
LNU54
24903.3
0.76
0.15
26
LNU44
24922.3
0.53
0.56
4


LNU19
25151.11
0.08
0.20
14
LNU79
24881.1
1.13
0.00
88
LNU54
24901.2
0.60
0.01
18


LNU27
24873.4
0.13
0.00
94
LNU79
24884.4
0.82
0.03
36
LNU54
24902.4
0.56
0.26
9


LNU27
24871.4
0.09
0.02
35
LNU79
24884.3
0.70
0.28
17
LNU54
24903.5
0.56
0.16
8


LNU27
24873.1
0.08
0.12
16
LNU79
24882.2
0.69
0.45
14
LNU54
24903.3
0.53
0.67
3


LNU44
24922.3
0.11
0.00
69
CONT.
—
0.70
—
0
LNU6
24992.3
0.60
0.08
17


LNU44
24924.3
0.09
0.01
38
LNU109
24892.6
1.14
0.00
64
LNU6
24994.2
0.60
0.02
17


LNU44
24923.1
0.08
0.03
24
LNU109
24892.5
0.98
0.05
41
LNU6
24994.5
0.58
0.11
12


LNU44
24924.2
0.07
0.75
4
LNU109
24891.2
0.88
0.06
26
LNU79
24882.2
0.57
0.18
10


LNU54
24903.5
0.13
0.00
100
LNU109
24891.5
0.86
0.09
23
LNU79
24881.1
0.56
0.27
9


LNU54
24902.4
0.10
0.02
54
LNU109
24892.8
0.72
0.78
3
LNU79
24883.2
0.56
0.18
8


LNU54
24901.2
0.09
0.04
36
LNU110
24952.1
1.18
0.00
69
LNU79
24884.4
0.55
0.31
7


LNU6
24994.5
0.12
0.00
85
LNU110
24954.1
1.06
0.00
51
CONT.
—
0.47
—
0


LNU6
24992.3
0.12
0.00
84
LNU110
24953.2
1.04
0.03
49
LNU109
24892.8
0.59
0.00
25


LNU6
24994.2
0.09
0.06
30
LNU110
24952.3
0.99
0.02
42
LNU109
24892.6
0.51
0.44
8


LNU6
24994.1
0.09
0.13
28
LNU110
24954.3
0.98
0.01
40
LNU109
24891.5
0.51
0.35
8


LNU79
24882.2
0.12
0.00
76
LNU133
24744.3
1.15
0.00
65
LNU143
25972.1
0.56
0.11
18


LNU79
24881.1
0.11
0.00
68
LNU133
24741.2
1.09
0.00
56
LNU143
25975.3
0.53
0.04
12


LNU79
24884.4
0.10
0.01
55
LNU133
24741.1
1.05
0.00
51
LNU143
25971.5
0.52
0.18
11


LNU79
24883.2
0.10
0.01
52
LNU133
24742.2
0.92
0.04
33
LNU143
25975.2
0.50
0.60
5


LNU79
24884.3
0.10
0.01
49
LNU133
24744.2
0.87
0.08
25
LNU154
14601.6
0.55
0.04
17


CONT.
—
0.05
—
0
LNU19
25151.1
1.04
0.00
50
LNU154
14604.6
0.50
0.48
6


LNU109
24892.8
0.11
0.00
100
LNU19
25151.11
0.88
0.04
27
LNU154
14604.5
0.49
0.68
3


LNU109
24891.5
0.09
0.02
61
LNU19
25153.3
0.88
0.13
27
LNU196
25532.2
0.53
0.19
13


LNU109
24891.2
0.08
0.04
47
LNU27
24873.4
1.01
0.02
45
LNU196
25534.1
0.52
0.29
10


LNU109
24892.6
0.06
0.63
10
LNU27
24873.1
0.92
0.03
32
LNU207
24641.1
0.55
0.04
17


LNU143
25975.2
0.07
0.01
35
LNU27
24871.4
0.92
0.05
32
LNU207
24644.18
0.52
0.30
11


LNU143
25972.1
0.07
0.14
33
LNU44
24922.3
1.19
0.00
71
LNU207
24642.5
0.52
0.30
10


LNU143
25975.3
0.06
0.22
14
LNU44
24923.3
0.97
0.05
39
LNU207
24642.4
0.51
0.40
9


LNU154
14604.7
0.09
0.00
55
LNU44
24924.3
0.76
0.50
9
LNU207
24644.13
0.51
0.27
8


LNU154
14601.6
0.07
0.14
27
LNU44
24923.1
0.74
0.63
6
LNU288
14562.7
0.55
0.04
17


LNU154
14604.6
0.07
0.15
27
LNU54
24903.5
1.09
0.00
56
LNU288
14562.9
0.51
0.43
9


LNU154
14602.8
0.07
0.18
19
LNU54
24902.4
0.88
0.20
26
LNU288
14564.9
0.50
0.48
5


LNU196
25532.2
0.11
0.01
98
LNU54
24901.2
0.84
0.25
21
LNU288
14562.1
0.49
0.71
3


LNU196
25534.1
0.09
0.00
59
LNU54
24903.3
0.76
0.41
10
LNU50
26025.4
0.53
0.09
13


LNU196
25531.2
0.06
0.40
16
LNU6
24992.3
1.13
0.00
62
LNU50
26024.2
0.53
0.08
12


LNU207
24642.5
0.10
0.00
82
LNU6
24994.5
1.11
0.02
59
LNU50
26023.2
0.52
0.18
11


LNU207
24642.4
0.07
0.05
32
LNU6
24994.2
1.03
0.01
47
LNU52
25723.2
0.50
0.56
6


LNU207
24644.18
0.07
0.15
22
LNU6
24994.1
0.77
0.54
10
LNU52
25721.3
0.50
0.63
6


LNU207
24644.13
0.06
0.39
14
LNU79
24884.4
1.05
0.02
51
CONT.
—
0.44
—
0


LNU207
24641.1
0.06
0.42
10
LNU79
24881.1
1.02
0.02
46
LNU143
25971.2
0.56
0.03
28


LNU288
14564.9
0.08
0.01
46
LNU79
24883.2
0.98
0.01
41
LNU143
25975.3
0.54
0.05
23


LNU288
14562.12
0.07
0.11
31
LNU79
24884.3
0.98
0.03
40
LNU143
25975.2
0.52
0.07
19


LNU288
14562.7
0.07
0.18
21
LNU79
24882.2
0.96
0.01
38
LNU143
25971.5
0.51
0.22
16


LNU288
14562.1
0.07
0.14
20
CONT.
—
0.59
—
0
LNU143
25972.1
0.47
0.52
8


LNU288
14562.9
0.07
0.15
20
LNU109
24892.8
0.94
0.00
59
LNU154
14601.6
0.59
0.00
36


LNU50
26024.2
0.08
0.01
50
LNU109
24891.5
0.87
0.00
47
LNU207
24641.1
0.58
0.06
33


LNU50
26025.4
0.07
0.04
34
LNU143
25975.2
0.75
0.05
27
LNU207
24642.5
0.53
0.08
22


LNU50
26023.5
0.06
0.80
4
LNU143
25972.1
0.67
0.43
13
LNU207
24642.4
0.49
0.26
13


LNU52
25721.3
0.09
0.01
59
LNU143
25975.3
0.66
0.39
12
LNU207
24644.15
0.49
0.42
12


LNU52
25723.2
0.08
0.00
54
LNU154
14604.7
0.77
0.03
30
LNU211
24771.1
0.58
0.01
33


LNU52
25721.4
0.07
0.18
29
LNU154
14601.6
0.73
0.15
23
LNU211
24774.4
0.47
0.49
8


CONT.
—
0.07
—
0
LNU196
25532.2
0.86
0.15
45
LNU211
24771.3
0.47
0.48
8


LNU154
14601.6
0.08
0.38
25
LNU196
25534.1
0.78
0.01
33
LNU211
24773.7
0.46
0.77
4


LNU207
24642.5
0.10
0.00
47
LNU207
24642.5
0.77
0.16
31
LNU52
25723.1
0.57
0.01
30


LNU207
24642.4
0.08
0.27
17
LNU207
24642.4
0.75
0.12
27
LNU52
25721.1
0.47
0.44
8


LNU52
25721.4
0.09
0.02
40
LNU207
24644.18
0.72
0.29
22
LNU69
14571.1
0.57
0.04
30


LNU52
25721.1
0.08
0.11
24
LNU207
24644.13
0.67
0.27
14
LNU69
14573.3
0.51
0.12
17


LNU52
25723.1
0.08
0.31
15
LNU207
24641.1
0.65
0.59
10
LNU69
14572.8
0.51
0.23
16


LNU69
14571.1
0.08
0.11
25
LNU288
14562.9
0.78
0.13
31
CONT.
—
0.39
—
0


LNU69
14572.8
0.07
0.68
7
LNU288
14564.9
0.70
0.26
18
LNU150
24842.9
0.59
0.01
51


CONT.
—
0.06
—
0
LNU288
14562.7
0.69
0.37
17
LNU150
24841.9
0.47
0.25
21


LNU150
24843.5
0.09
0.15
32
LNU288
14562.12
0.63
0.69
7
LNU150
24843.9
0.46
0.33
19


LNU150
24841.9
0.09
0.15
32
LNU50
26025.4
0.84
0.00
43
LNU150
24842.5
0.46
0.32
18


LNU150
24842.9
0.08
0.22
26
LNU50
26024.2
0.83
0.02
41
LNU150
24843.5
0.45
0.40
16


LNU150
24843.9
0.07
0.49
15
LNU50
26023.5
0.70
0.26
18
LNU179
24631.9
0.57
0.02
45


LNU232
26003.7
0.08
0.20
28
LNU50
26023.2
0.62
0.69
5
LNU179
24632.7
0.48
0.20
24


LNU235
26184.4
0.07
0.73
7
LNU52
25723.2
0.82
0.01
39
LNU179
24631.6
0.47
0.24
21


LNU242
25474.1
0.09
0.04
45
LNU52
25721.4
0.70
0.29
19
LNU179
24631.7
0.45
0.37
17


LNU242
25473.1
0.08
0.27
25
LNU52
25721.3
0.70
0.22
18
LNU179
24632.5
0.43
0.60
10


LNU242
25471.1
0.07
0.56
12
CONT.
—
0.70
—
0
LNU232
26003.3
0.53
0.06
36


LNU76
26421.2
0.09
0.13
36
LNU143
25975.3
0.81
0.31
16
LNU232
26003.7
0.50
0.11
29


LNU76
26422.2
0.07
0.69
9
LNU143
25975.2
0.81
0.31
16
LNU232
26001.5
0.46
0.35
17


LNU76
26421.1
0.07
0.76
7
LNU154
14601.6
0.96
0.18
37
LNU232
26001.2
0.45
0.39
16


LNU95
13985.15
0.08
0.21
28
LNU207
24641.1
0.86
0.30
23
LNU232
26003.6
0.44
0.47
14


LNU95
13985.11
0.08
0.33
21
LNU207
24642.5
0.83
0.22
19
LNU235
26184.4
0.50
0.13
28


CONT.
—
0.07
—
0
LNU207
24642.4
0.81
0.33
16
LNU235
26185.3
0.49
0.17
25


LNU118
14013.8
0.09
0.03
36
LNU211
24771.1
0.84
0.24
21
LNU235
26184.2
0.48
0.22
23


LNU118
14013.6
0.09
0.07
29
LNU52
25723.1
0.86
0.23
24
LNU235
26182.1
0.47
0.27
20


LNU118
14012.15
0.08
0.25
19
LNU52
25721.1
0.73
0.76
5
LNU235
26185.2
0.43
0.55
11


LNU118
14012.12
0.08
0.29
16
LNU69
14571.1
0.90
0.13
30
LNU242
25473.3
0.56
0.02
43


LNU118
14012.14
0.07
0.60
9
LNU69
14573.3
0.76
0.62
9
LNU242
25474.1
0.54
0.05
39


LNU150
24842.9
0.10
0.00
54
LNU69
14572.8
0.75
0.63
8
LNU242
25471.1
0.51
0.08
32


LNU150
24841.9
0.08
0.07
28
CONT.
—
0.47
—
0
LNU242
25473.1
0.51
0.09
31


LNU150
24841.6
0.07
0.69
6
LNU150
24842.9
0.83
0.00
76
LNU242
25472.1
0.41
0.73
6


LNU179
24632.7
0.08
0.09
28
LNU150
24843.5
0.79
0.00
68
LNU76
26421.2
0.55
0.04
42


LNU179
24631.7
0.08
0.20
20
LNU150
24841.9
0.74
0.00
58
LNU76
26421.1
0.49
0.14
27


LNU179
24632.5
0.07
0.68
7
LNU150
24843.9
0.62
0.06
33
LNU76
26422.2
0.43
0.57
11


LNU232
26001.5
0.09
0.06
30
LNU179
24631.9
0.71
0.00
51
LNU76
26423.1
0.43
0.58
10


LNU232
26003.3
0.07
0.51
10
LNU179
24632.5
0.56
0.24
21
LNU76
26425.1
0.42
0.66
8


LNU232
26003.6
0.07
0.65
7
LNU232
26003.7
0.82
0.00
75
LNU95
13985.11
0.53
0.07
35


LNU235
26184.4
0.12
0.00
76
LNU232
26003.3
0.68
0.01
45
LNU95
13985.15
0.50
0.12
29


LNU235
26185.2
0.09
0.01
40
LNU232
26003.6
0.51
0.64
9
LNU95
13985.19
0.48
0.19
25


LNU235
26184.2
0.09
0.02
37
LNU235
26185.3
0.63
0.05
34
LNU95
13985.16
0.48
0.22
23


LNU235
26182.1
0.07
0.67
6
LNU235
26184.4
0.62
0.07
32
LNU95
13985.12
0.46
0.34
18


LNU242
25474.1
0.08
0.12
24
LNU235
26182.1
0.54
0.35
15
CONT.
—
0.44
—
0


LNU288
14563.9
0.12
0.00
76
LNU235
26184.2
0.54
0.42
15
LNU118
14013.6
0.48
0.65
9


LNU288
14562.1
0.09
0.07
31
LNU242
25474.1
0.87
0.00
86
LNU118
14012.12
0.47
0.77
6


LNU288
14563.6
0.08
0.14
23
LNU242
25471.1
0.69
0.01
47
LNU150
24842.9
0.54
0.27
22


LNU288
14564.9
0.08
0.20
23
LNU242
25473.1
0.68
0.01
46
LNU150
24843.5
0.47
0.74
6


LNU288
14562.7
0.08
0.40
14
LNU242
25473.3
0.56
0.26
19
LNU179
24632.5
0.50
0.47
13


LNU76
26421.2
0.08
0.18
21
LNU242
25472.1
0.51
0.65
8
LNU179
24631.7
0.48
0.64
9


LNU76
26423.1
0.07
0.48
11
LNU76
26421.2
0.84
0.00
80
LNU232
26001.5
0.52
0.40
16


LNU76
26422.2
0.07
0.60
8
LNU76
26421.1
0.67
0.01
43
LNU232
26003.3
0.52
0.39
16


LNU76
26425.1
0.07
0.63
8
LNU76
26422.2
0.54
0.37
15
LNU235
26185.2
0.52
0.38
16


LNU95
13985.16
0.11
0.00
66
LNU76
26425.1
0.53
0.48
13
LNU235
26184.4
0.49
0.57
11


LNU95
13985.15
0.10
0.01
44
LNU76
26423.1
0.51
0.65
8
LNU235
26184.2
0.48
0.64
9


LNU95
13985.12
0.08
0.10
26
LNU95
13985.11
0.86
0.00
83
LNU235
26182.1
0.48
0.65
9


CONT.
—
0.06
—
0
LNU95
13985.15
0.81
0.00
73
LNU242
25474.1
0.53
0.33
18


LNU101
27635.1
0.07
0.03
24
LNU95
13985.19
0.67
0.03
43
LNU288
14563.9
0.54
0.27
21


LNU101
27632.1
0.07
0.18
14
LNU95
13985.16
0.66
0.03
42
LNU288
14563.6
0.51
0.43
15


LNU128
26515.3
0.08
0.00
35
LNU95
13985.12
0.54
0.40
16
LNU288
14562.1
0.49
0.63
9


LNU128
26515.2
0.07
0.15
18
CONT.
—
0.58
—
0
LNU288
14562.7
0.48
0.70
7


LNU192
28315.2
0.07
0.06
22
LNU118
14013.6
0.77
0.08
33
LNU76
26421.2
0.49
0.60
10


LNU192
28313.2
0.07
0.36
11
LNU118
14012.15
0.76
0.14
31
LNU76
26425.1
0.48
0.68
8


LNU211
24771.1
0.08
0.00
37
LNU118
14013.8
0.61
0.78
5
LNU95
13985.11
0.55
0.21
23


LNU282
27563.3
0.08
0.00
34
LNU150
24842.9
0.84
0.03
45
LNU95
13985.12
0.50
0.49
13


LNU69
14571.1
0.08
0.00
39
LNU150
24843.5
0.71
0.20
23
LNU95
13985.16
0.49
0.58
10


LNU69
14573.5
0.07
0.22
13
LNU150
24841.9
0.68
0.34
18
LNU95
13985.15
0.48
0.71
7


LNU69
14572.9
0.06
0.60
5
LNU179
24632.7
0.65
0.49
13
CONT.
—
0.52
—
0


LNU75
27572.1
0.08
0.00
36
LNU179
24631.7
0.64
0.60
10
LNU101
27632.5
0.64
0.01
24


LNU75
27572.2
0.07
0.18
15
LNU179
24632.5
0.61
0.76
6
LNU101
27635.1
0.57
0.31
9


LNU75
27572.3
0.06
0.58
6
LNU232
26001.5
0.93
0.00
60
LNU101
27632.1
0.55
0.50
6


CONT.
—
0.05
—
0
LNU232
26003.3
0.83
0.02
42
LNU101
27632.6
0.55
0.54
6


LNU101
27632.5
0.08
0.03
38
LNU235
26184.4
1.17
0.00
101
LNU128
26511.4
0.59
0.15
14


LNU118
14012.15
0.07
0.16
23
LNU235
26185.2
0.88
0.01
52
LNU128
26511.5
0.54
0.70
4


LNU118
14013.6
0.07
0.14
23
LNU235
26184.2
0.85
0.01
47
LNU192
28313.3
0.63
0.01
22


LNU118
14013.9
0.06
0.54
8
LNU235
26182.1
0.68
0.32
18
LNU192
28315.2
0.63
0.04
21


LNU206
27621.2
0.07
0.01
38
LNU242
25474.1
0.88
0.01
51
LNU192
28313.2
0.56
0.49
7


LNU206
27621.1
0.06
0.38
13
LNU288
14563.9
0.99
0.00
71
LNU206
27621.2
0.59
0.24
13


LNU249
26153.1
0.09
0.00
57
LNU288
14562.1
0.80
0.04
38
LNU282
27563.3
0.54
0.69
4


LNU282
27563.1
0.07
0.05
33
LNU288
14563.6
0.73
0.17
26
CONT.
—
0.50
—
0


LNU282
27565.2
0.06
0.79
4
LNU288
14562.7
0.61
0.78
5
LNU101
27635.1
0.58
0.02
17


LNU288
14564.8
0.09
0.00
67
LNU76
26421.2
0.66
0.43
14
LNU101
27632.5
0.56
0.13
12


LNU288
14563.9
0.08
0.00
56
LNU76
26423.1
0.63
0.60
9
LNU118
14012.15
0.56
0.13
11


LNU288
14562.9
0.07
0.04
35
LNU76
26425.1
0.63
0.62
9
LNU118
14013.6
0.51
0.73
3


LNU288
14562.7
0.07
0.13
24
LNU76
26422.2
0.63
0.66
8
LNU128
26515.3
0.60
0.01
19


LNU288
14563.6
0.06
0.33
15
LNU95
13985.15
0.87
0.01
51
LNU192
28315.2
0.54
0.30
9


LNU75
27571.4
0.08
0.03
42
LNU95
13985.16
0.86
0.01
48
LNU206
27621.2
0.58
0.03
17


LNU75
27572.3
0.07
0.07
33
LNU95
13985.12
0.73
0.16
26
LNU206
27622.4
0.53
0.37
6


LNU75
27572.2
0.07
0.08
30
CONT.
—
0.61
—
0
LNU249
26152.2
0.58
0.03
16


LNU75
27571.2
0.06
0.23
17
LNU101
27632.1
0.83
0.00
36
LNU249
26152.4
0.55
0.26
11


CONT.
—
0.06
—
0
LNU101
27635.1
0.77
0.02
25
LNU249
26151.1
0.52
0.68
4


LNU11
28204.3
0.07
0.63
6
LNU101
27632.5
0.73
0.13
19
LNU249
26153.1
0.52
0.70
4


LNU11
28204.1
0.07
0.73
4
LNU101
27632.6
0.67
0.47
9
LNU282
27563.3
0.56
0.09
12


LNU112
28212.4
0.07
0.64
6
LNU128
26515.3
0.95
0.00
55
LNU282
27563.1
0.55
0.26
11


LNU14
27824.2
0.08
0.22
32
LNU128
26515.2
0.82
0.00
35
LNU282
27562.1
0.55
0.42
10


LNU183
24863.12
0.12
0.00
81
LNU128
26511.4
0.76
0.06
24
LNU282
27565.2
0.53
0.41
6


LNU183
24863.1
0.10
0.00
50
LNU128
26511.5
0.63
0.77
3
LNU282
27565.1
0.51
0.79
2


LNU183
24865.1
0.09
0.00
46
LNU192
28315.2
0.95
0.00
56
LNU288
14563.6
0.62
0.01
24


LNU183
24864.6
0.07
0.25
14
LNU192
28313.2
0.88
0.00
44
LNU288
14564.8
0.60
0.01
19


LNU191
28323.1
0.07
0.70
6
LNU192
28313.3
0.74
0.04
21
LNU288
14562.9
0.56
0.12
12


LNU201
28223.1
0.07
0.71
4
LNU206
27621.2
0.83
0.00
36
LNU288
14562.7
0.51
0.67
3


LNU268
26044.2
0.08
0.20
18
LNU206
27621.1
0.72
0.14
17
LNU75
27572.2
0.61
0.00
23


LNU268
26045.1
0.07
0.32
12
LNU211
24771.1
0.81
0.01
32
LNU75
27571.4
0.60
0.03
20


CONT.
—
0.05
—
0
LNU282
27563.3
0.79
0.02
30
LNU75
27572.1
0.57
0.05
15


LNU11
28204.1
0.07
0.08
32
LNU69
14571.1
0.79
0.01
29
LNU75
27571.2
0.54
0.30
8


LNU11
28205.2
0.07
0.09
29
LNU69
14573.5
0.78
0.02
28
CONT.
—
0.50
—
0


LNU11
28205.1
0.07
0.13
29
LNU75
27572.2
0.94
0.00
53
LNU11
28203.2
0.55
0.43
11


LNU11
28203.2
0.06
0.12
25
LNU75
27572.3
0.81
0.00
33
LNU11
28202.5
0.55
0.45
10


LNU11
28204.3
0.06
0.25
18
LNU75
27572.1
0.78
0.02
27
LNU112
28212.1
0.62
0.09
24


LNU11
28202.5
0.05
0.78
5
LNU75
27571.4
0.70
0.25
14
LNU112
28212.4
0.52
0.72
5


LNU112
28212.4
0.07
0.03
40
CONT.
—
0.61
—
0
LNU14
27823.2
0.56
0.37
13


LNU112
28212.1
0.06
0.22
22
LNU101
27632.5
0.86
0.03
42
LNU14
27821.1
0.55
0.49
10


LNU112
28211.2
0.06
0.52
11
LNU101
27635.1
0.65
0.69
7
LNU183
24864.6
0.56
0.38
13


LNU14
27821.3
0.07
0.07
38
LNU118
14012.15
0.77
0.11
26
LNU201
28223.1
0.57
0.27
15


LNU14
27821.4
0.07
0.17
29
LNU118
14013.6
0.74
0.19
21
LNU201
28222.3
0.55
0.40
11


LNU14
27823.2
0.07
0.13
27
LNU118
14013.9
0.65
0.60
8
LNU201
28223.3
0.52
0.76
4


LNU183
24865.1
0.14
0.00
176
LNU206
27621.2
0.88
0.01
45
LNU268
26044.2
0.58
0.25
16


LNU183
24863.12
0.13
0.00
158
LNU249
26153.1
0.76
0.10
26
LNU268
26041.6
0.54
0.52
8


LNU183
24863.1
0.13
0.00
156
LNU249
26152.4
0.70
0.38
15
LNU268
26041.4
0.53
0.68
6


LNU183
24864.6
0.13
0.00
147
LNU282
27563.1
0.74
0.21
22
LNU268
26045.1
0.52
0.78
4


LNU183
24864.7
0.06
0.21
22
LNU282
27565.2
0.64
0.74
5
CONT.
—
0.60
—
0


LNU191
28325.4
0.08
0.02
49
LNU288
14564.8
0.86
0.01
42
LNU11
28205.2
0.67
0.31
12


LNU191
28324.2
0.07
0.04
42
LNU288
14563.6
0.83
0.05
37
LNU11
28204.3
0.65
0.43
9


LNU191
28323.1
0.07
0.16
37
LNU288
14562.9
0.81
0.04
34
LNU11
28204.1
0.63
0.57
6


LNU191
28325.3
0.07
0.06
34
LNU288
14563.9
0.75
0.16
24
LNU11
28203.2
0.63
0.60
6


LNU191
28321.3
0.06
0.57
10
LNU75
27572.2
0.90
0.01
49
LNU112
28212.4
0.62
0.70
4


LNU201
28222.2
0.09
0.00
82
LNU75
27571.4
0.86
0.03
41
LNU14
27821.3
0.64
0.45
8


LNU201
28223.3
0.06
0.20
23
LNU75
27571.2
0.80
0.04
31
LNU183
24863.1
0.72
0.08
21


LNU201
28221.3
0.06
0.42
14
LNU75
27572.3
0.74
0.29
21
LNU183
24864.6
0.63
0.63
5


LNU201
28223.1
0.06
0.48
13
CONT.
—
0.81
—
0
LNU201
28222.2
0.63
0.59
6


LNU201
28222.3
0.05
0.72
6
LNU14
27821.3
0.92
0.41
14
LNU268
26043.4
0.63
0.55
7


LNU268
26043.4
0.08
0.01
47
LNU183
24863.12
0.84
0.78
4
CONT.
—
0.53
—
0


LNU268
26041.4
0.07
0.03
39
LNU268
26044.2
1.01
0.17
25
LNU116
14492.5
0.58
0.46
10


LNU268
26041.6
0.06
0.31
22
CONT.
—
0.75
—
0
LNU121
27713.4
0.55
0.76
4


LNU268
26045.1
0.06
0.48
14
LNU11
28205.2
1.10
0.00
46
LNU126
25343.3
0.57
0.59
7


LNU268
26044.2
0.06
0.68
7
LNU11
28203.2
0.99
0.04
32
LNU158
27433.3
0.60
0.38
13


CONT.
—
0.04
—
0
LNU11
28205.1
0.94
0.09
25
LNU177
24765.2
0.60
0.38
14


LNU107
14585.5
0.06
0.17
32
LNU11
28204.3
0.93
0.10
24
LNU177
24762.6
0.56
0.67
5


LNU107
14584.9
0.05
0.31
18
LNU11
28202.5
0.91
0.15
21
LNU177
24764.9
0.56
0.69
5


LNU107
14583.8
0.05
0.44
14
LNU11
28204.1
0.82
0.48
9
LNU182
25384.5
0.58
0.48
10


LNU116
14493.6
0.06
0.02
38
LNU112
28212.4
0.89
0.22
18
LNU2
27842.3
0.60
0.35
13


LNU116
14494.5
0.06
0.07
30
LNU112
28212.1
0.85
0.35
12
LNU225
25991.2
0.59
0.41
12


LNU116
14492.9
0.05
0.02
28
LNU14
27821.3
1.08
0.01
44
LNU225
25991.5
0.59
0.47
11


LNU116
14492.5
0.05
0.13
21
LNU14
27823.2
0.85
0.46
14
LNU239
26284.1
0.60
0.32
14


LNU121
25642.2
0.08
0.00
99
LNU183
24865.1
1.33
0.00
11
LNU239
26284.2
0.57
0.57
1


LNU121
27713.4
0.07
0.00
60
LNU183
24863.1
1.32
0.00
75
LNU57
27852.1
0.59
0.39
12


LNU121
27711.1
0.06
0.04
40
LNU183
24864.6
1.09
0.01
45
LNU83
27684.1
0.56
0.64
7


LNU121
27713.1
0.06
0.07
36
LNU183
24863.12
1.09
0.01
45
LNU83
27682.1
0.55
0.76
4


LNU126
25345.1
0.07
0.00
57
LNU191
28323.1
0.97
0.15
29
CONT.
—
0.51
—
0


LNU126
25343.3
0.07
0.00
57
LNU191
28325.4
0.81
0.54
8
LNU107
14584.9
0.66
0.14
29


LNU126
25343.1
0.06
0.05
40
LNU201
28222.2
1.26
0.00
67
LNU107
14583.8
0.64
0.14
26


LNU158
27433.3
0.06
0.05
33
LNU201
28223.1
0.82
0.50
10
LNU107
14585.2
0.61
0.22
20


LNU158
27433.2
0.05
0.28
19
LNU268
26043.4
1.00
0.03
33
LNU107
14585.5
0.58
0.40
13


LNU158
27432.5
0.05
0.64
10
LNU268
26041.4
0.91
0.14
21
LNU116
14491.5
0.61
0.28
19


LNU177
24765.2
0.05
0.08
28
LNU268
26041.6
0.90
0.31
20
LNU116
14492.9
0.55
0.61
8


LNU177
24762.6
0.05
0.15
26
LNU268
26044.2
0.80
0.61
6
LNU121
27713.1
0.68
0.05
33


LNU177
24764.9
0.05
0.54
8
CONT.
—
0.61
—
0
LNU121
27713.4
0.63
0.20
22


LNU182
25384.5
0.06
0.03
30
LNU107
14585.5
0.74
0.27
21
LNU121
27711.1
0.62
0.18
21


LNU182
27521.4
0.05
0.22
27
LNU107
14583.8
0.74
0.20
20
LNU126
25343.1
0.63
0.17
22


LNU182
25384.1
0.05
0.53
11
LNU107
14584.9
0.69
0.32
13
LNU126
25343.4
0.62
0.18
21


LNU2
25713.1
0.05
0.06
29
LNU116
14492.5
0.67
0.43
10
LNU126
25343.3
0.53
0.80
4


LNU2
27842.1
0.05
0.59
15
LNU116
14492.9
0.65
0.59
7
LNU158
27433.3
0.69
0.06
35


LNU2
27842.3
0.05
0.55
9
LNU116
14494.5
0.65
0.61
7
LNU158
27433.2
0.66
0.09
29


LNU225
25991.5
0.05
0.46
14
LNU121
27713.4
0.96
0.00
57
LNU158
27432.5
0.65
0.11
27


LNU225
25991.2
0.05
0.55
9
LNU121
25642.2
0.87
0.02
43
LNU158
27434.1
0.61
0.17
19


LNU239
26284.1
0.06
0.01
40
LNU121
27711.1
0.85
0.01
39
LNU158
27434.5
0.56
0.56
10


LNU239
26283.2
0.05
0.41
12
LNU121
27713.1
0.69
0.34
12
LNU177
24763.6
0.68
0.07
33


LNU239
26281.1
0.05
0.63
6
LNU126
25345.1
0.89
0.00
46
LNU177
24764.12
0.66
0.07
29


LNU57
27852.1
0.05
0.23
15
LNU126
25343.3
0.80
0.02
31
LNU177
24765.2
0.64
0.12
25


LNU57
27854.3
0.05
0.35
12
LNU126
25343.1
0.75
0.14
23
LNU177
24764.9
0.58
0.38
14


CONT.
—
0.03
—
0
LNU158
27433.3
1.04
0.01
71
LNU182
25384.2
0.67
0.08
31


LNU107
14584.9
0.06
0.00
151
LNU158
27433.2
0.94
0.00
54
LNU182
25384.1
0.60
0.24
18


LNU107
14585.2
0.05
0.02
105
LNU158
27432.5
0.71
0.37
16
LNU182
25384.5
0.59
0.30
16


LNU107
14585.5
0.04
0.09
64
LNU177
24762.6
1.04
0.00
71
LNU182
25384.6
0.59
0.37
16


LNU107
14583.8
0.04
0.31
41
LNU177
24765.2
0.83
0.11
35
LNU182
27521.4
0.55
0.63
8


LNU107
14583.1
0.03
0.35
34
LNU177
24764.9
0.69
0.28
13
LNU2
27842.1
0.65
0.14
26


LNU116
14492.5
0.06
0.01
126
LNU182
25384.5
0.91
0.01
49
LNU2
27842.3
0.62
0.20
21


LNU116
14493.6
0.05
0.05
81
LNU182
27521.4
0.84
0.02
38
LNU2
25713.1
0.58
0.36
13


LNU116
14491.5
0.05
0.10
77
LNU182
25384.1
0.80
0.03
31
LNU225
25991.3
0.65
0.12
28


LNU116
14494.5
0.03
0.59
26
LNU2
27842.3
0.90
0.01
47
LNU225
25991.2
0.62
0.21
22


LNU116
14492.9
0.03
0.72
15
LNU2
25713.1
0.85
0.01
39
LNU225
25991.5
0.62
0.22
21


LNU121
27713.4
0.09
0.00
258
LNU2
27842.1
0.77
0.31
26
LNU225
25991.8
0.59
0.34
16


LNU121
27713.1
0.06
0.01
145
LNU225
25991.5
1.09
0.00
78
LNU239
26283.3
0.59
0.33
16


LNU121
27713.3
0.05
0.01
113
LNU225
25991.2
0.81
0.05
32
LNU239
26284.1
0.58
0.37
14


LNU121
25642.2
0.05
0.01
99
LNU225
25991.1
0.75
0.15
22
LNU239
26281.1
0.58
0.42
14


LNU121
27711.1
0.05
0.10
79
LNU225
25991.3
0.68
0.55
12
LNU239
26284.2
0.54
0.70
6


LNU126
25343.1
0.05
0.01
111
LNU239
26284.1
0.78
0.06
28
LNU57
27854.5
0.66
0.11
29


LNU126
25343.3
0.04
0.14
66
LNU239
26283.2
0.68
0.48
12
LNU57
27852.1
0.64
0.12
26


LNU126
25343.4
0.03
0.53
20
LNU239
26281.1
0.67
0.48
9
LNU57
27851.2
0.63
0.16
23


LNU126
25345.1
0.03
0.53
19
LNU57
27852.1
0.92
0.00
50
LNU57
27854.3
0.59
0.29
16


LNU158
27432.5
0.07
0.00
170
LNU57
27854.3
0.71
0.25
16
LNU83
27685.1
0.71
0.05
39


LNU158
27433.3
0.07
0.00
159
LNU57
27854.5
0.68
0.39
12
LNU83
27682.1
0.66
0.11
30


LNU158
27433.2
0.05
0.02
105
LNU83
27681.4
0.85
0.02
38
LNU83
27685.2
0.66
0.08
29


LNU158
27434.1
0.03
0.56
21
LNU83
27684.1
0.66
0.60
8
LNU83
27681.4
0.59
0.34
15


LNU158
27434.5
0.03
0.67
16
LNU83
27685.1
0.64
0.78
4
LNU83
27684.1
0.58
0.39
14


LNU177
24762.6
0.04
0.09
60
CONT.
—
0.58
—
0







LNU177
24764.12
0.03
0.41
30
LNU107
14584.9
1.26
0.00
118







LNU177
24763.6
0.03
0.51
26
LNU107
14583.8
0.96
0.00
66







LNU182
25384.1
0.05
0.02
103
LNU107
14585.2
0.84
0.00
45







LNU182
25384.6
0.05
0.04
101
LNU107
14583.1
0.64
0.40
11







LNU182
25384.2
0.04
0.08
72
LNU107
14585.5
0.63
0.56
8







LNU182
25384.5
0.03
0.43
31
LNU116
14493.6
0.91
0.00
56







LNU182
27521.4
0.03
0.76
11
LNU116
14494.5
0.86
0.02
48







LNU2
27845.3
0.05
0.02
105
LNU116
14491.5
0.86
0.03
48







LNU2
25713.1
0.05
0.04
95
LNU116
14492.5
0.73
0.06
26







LNU2
27842.3
0.05
0.09
85
LNU116
14492.9
0.63
0.64
9







LNU2
27842.1
0.04
0.09
72
LNU121
27713.4
1.28
0.00
120







LNU2
27845.2
0.03
0.46
30
LNU121
27713.1
1.13
0.00
95







LNU225
25991.2
0.08
0.00
207
LNU121
27711.1
0.85
0.01
47







LNU225
25991.3
0.05
0.03
110
LNU121
25642.2
0.70
0.23
20







LNU225
25991.1
0.05
0.05
80
LNU126
25343.1
0.85
0.02
46







LNU225
25991.8
0.04
0.12
68
LNU126
25343.4
0.68
0.24
17







LNU225
25991.5
0.03
0.58
19
LNU126
25343.3
0.66
0.35
14







LNU239
26284.2
0.05
0.02
102
LNU126
25345.1
0.63
0.56
9







LNU239
26281.1
0.04
0.11
71
LNU158
27433.3
1.25
0.00
117







LNU239
26283.2
0.04
0.11
67
LNU158
27432.5
1.22
0.00
110







LNU239
26283.3
0.04
0.27
53
LNU158
27433.2
0.88
0.00
52







LNU239
26284.1
0.04
0.27
44
LNU158
27434.5
0.84
0.01
46







LNU57
27852.1
0.05
0.07
81
LNU158
27434.1
0.71
0.15
23







LNU57
27851.2
0.04
0.08
62
LNU177
24764.12
0.96
0.00
65







LNU57
27854.5
0.03
0.43
27
LNU177
24763.6
0.82
0.01
42







LNU83
27685.1
0.05
0.04
110
LNU177
24765.2
0.63
0.58
9







LNU83
27685.2
0.05
0.03
91
LNU182
25384.6
1.03
0.00
78







LNU83
27681.4
0.04
0.17
53
LNU182
25384.1
0.91
0.00
58







LNU83
27682.1
0.03
0.41
34
LNU182
25384.2
0.84
0.01
46







LNU83
27684.1
0.03
0.69
16
LNU182
27521.4
0.71
0.16
23












LNU182
25384.5
0.70
0.18
21












LNU2
27842.1
0.98
0.00
69












LNU2
27842.3
0.95
0.00
64












LNU2
25713.1
0.89
0.02
54












LNU2
27845.2
0.68
0.27
18












LNU225
25991.2
1.35
0.00
133












LNU225
25991.3
1.27
0.00
119












LNU225
25991.8
0.87
0.01
50












LNU225
25991.1
0.82
0.00
42












LNU225
25991.5
0.82
0.01
41












LNU239
26283.3
0.88
0.01
51












LNU239
26281.1
0.82
0.02
42












LNU239
26284.2
0.70
0.15
20












LNU239
26284.1
0.66
0.33
14












LNU239
26283.2
0.65
0.36
12












LNU57
27854.5
1.11
0.00
92












LNU57
27851.2
1.11
0.00
91












LNU57
27852.1
1.08
0.00
86












LNU57
27854.3
0.69
0.16
19












LNU83
27685.1
1.10
0.00
89












LNU83
27685.2
1.02
0.00
76












LNU83
27682.1
0.88
0.01
51












LNU83
27681.4
0.83
0.00
43












LNU83
27684.1
0.68
0.35
17





Table 76.


“CONT.”—Control;


“Ave.”—Average;


“% Incr.” = % increment.













TABLE 77







Genes showing improved growth rate at standard nitrogen


growth conditions (T1 generation)















RGR Of Roots

RGR Of Roots



RGR Of Leaf Area

Coverage

Length


















Gene

p-
%
Gene

p-
%
Gene

p-
%


Name
Ave.
value
incr.
Name
Ave.
value
incr.
Name
Ave.
value
incr.





















CONT.
0.064
—
0.0
CONT.
0.308
—
0.0
CONT.
0.401
—
0


LNU121
0.070
0.313
8.9
LNU121
0.498
0.000
61.7
LNU121
0.480
0.002
19.8


CONT.
0.065
—
0.0
LNU154
0.341
0.475
10.6
LNU154
0.402
0.971
0.36


LNU275
0.103
0.000
59.6
LNU210
0.339
0.326
10.2
LNU210
0.417
0.531
4


LNU57
0.073
0.309
13.4
CONT.
0.322
—
0.0
CONT.
0.386
—
0


LNU64
0.068
0.723
4.5
LNU275
0.862
0.000
167.2
LNU275
0.509
0.002
32


LNU83
0.084
0.033
29.3
LNU57
0.535
0.000
66.0
LNU57
0.515
0.000
34


CONT.
0.063
—
0.0
LNU58
0.387
0.154
20.1
LNU58
0.407
0.473
5.7


LNU59
0.065
0.738
3.5
LNU83
0.549
0.000
70.2
LNU64
0.388
0.954
0.5


CONT.
0.229
—
0.0
CONT.
0.186
—
0.0
LNU83
0.528
0.000
37


LNU222_H6
0.258
0.65 
13
LNU176
0.250
0.043
34.6
CONT.
0.326
—
0






LNU214
0.219
0.210
18.2
LNU176
0.371
0.101
14






LNU223
0.234
0.060
26.2
LNU214
0.368
0.120
13






LNU233
0.230
0.129
23.9
LNU223
0.364
0.097
12






LNU245
0.200
0.513
7.7
LNU233
0.372
0.061
14






LNU247
0.187
0.961
0.7
LNU245
0.375
0.045
16






LNU284
0.222
0.220
19.5
LNU247
0.335
0.745
3






LNU289
0.241
0.020
30.1
LNU284
0.377
0.105
16






LNU70
0.247
0.038
32.9
LNU289
0.366
0.086
13






LNU85
0.256
0.013
38.2
LNU70
0.377
0.112
16






CONT.
0.380
—
0.0
LNU85
0.416
0.002
28






LNU105
0.400
0.710
5.2
CONT.
0.413
—
0






LNU115
0.432
0.278
13.6
LNU105
0.446
0.325
8.1






LNU134
0.434
0.339
14.2
LNU115
0.455
0.168
10






LNU198
0.385
0.925
1.2
LNU134
0.446
0.328
8






LNU200
0.391
0.820
3.0
LNU198
0.415
0.966
0.4






CONT.
0.838
—
0.0
CONT.
0.468
—
0






LNU51
0.395
0.870
1.8
LNU225
0.478
0.756
2.3






LNU59
0.402
0.703
3.7
LNU51
0.488
0.541
4.5






CONT.
0.429


LNU59
0.502
0.320
7.3






LNU258
0.522
0.25 
21.7





Table 77.


“CONT.”—Control;


“Ave.”—Average;


“% Incr.” = % increment.






Example 15
Evaluation of Transgenic Arabidopsis NUE, Yield and Plant Growth Rate Under Low or Normal Nitrogen Fertilization in Greenhouse Assay
Assay 1: Nitrogen Use efficiency: Seed yield plant biomass and plant growth rate at limited and optimal nitrogen concentration under greenhouse conditions—This assay follows seed yield production, the biomass formation and the rosette area growth of plants grown in the greenhouse at limiting and non-limiting nitrogen growth conditions. Transgenic Arabidopsis seeds were sown in agar media supplemented with 1⁄2 MS medium and a selection agent (Kanamycin). The T2 transgenic seedlings were then transplanted to 1.7 trays filled with peat and perlite in a 1:1 ratio. The trays were irrigated with a solution containing nitrogen limiting conditions, which were achieved by irrigating the plants with a solution containing 1.5 mM inorganic nitrogen in the form of KNO3, supplemented with 1 mM KH2PO4, 1 mM MgSO4, 3.6 mM KCl, 2 mM CaCl2 and microelements, while normal nitrogen levels were achieved by applying a solution of 6 mM inorganic nitrogen also in the form of KNO3 with 1 mM KH2PO4, 1 mM MgSO4, 2 mM CaCl2 and microelements. All plants were grown in the greenhouse until mature seeds. Seeds were harvested, extracted and weight. The remaining plant biomass (the above ground tissue) was also harvested, and weighted immediately or following drying in oven at 50° C. for 24 hours.
Each construct was validated at its T2 generation. Transgenic plants transformed with a construct conformed by an empty vector carrying the 35S promoter and the selectable marker was used as control.
The plants were analyzed for their overall size, growth rate, flowering, seed yield, 1,000-seed weight, dry matter and harvest index (HI—seed yield/dry matter).
Transgenic plants performance was compared to control plants grown in parallel under the same conditions. Mock-transgenic plants expressing the uidA reporter gene (GUS-Intron) or with no gene at all, under the same promoter were used as control.
The experiment was planned in nested randomized plot distribution. For each gene of the invention three to five independent transformation events were analyzed from each construct.
Digital imaging—A laboratory image acquisition system, which consists of a digital reflex camera (Canon EOS 300D) attached with a 55 mm focal length lens (Canon EF-S series), mounted on a reproduction device (Kaiser RS), which includes 4 light units (4×150 Watts light bulb) is used for capturing images of plant samples.
The image capturing process is repeated every 2 days starting from day 1 after transplanting till day 15. Same camera, placed in a custom made iron mount, is used for capturing images of larger plants sawn in white tubs in an environmental controlled greenhouse. The tubs are square shape include 1.7 liter trays. During the capture process, the tubs are placed beneath the iron mount, while avoiding direct sun light and casting of shadows.
An image analysis system is used, which consists of a personal desktop computer (Intel P4 3.0 GHz processor) and a public domain program—ImageJ 1.39 [Java based image processing program which was developed at the U.S. National Institutes of Health and freely available on the internet at Hypertext Transfer Protocol://rsbweb (dot) nih (dot) gov/]. Images are captured in resolution of 10 Mega Pixels (3888×2592 pixels) and stored in a low compression JPEG (Joint Photographic Experts Group standard) format. Next, analyzed data is saved to text files and processed using the JMP statistical analysis software (SAS institute).
Leaf analysis—Using the digital analysis leaves data is calculated, including leaf number, rosette area, rosette diameter, leaf blade area.
Vegetative growth rate: the relative growth rate (RGR) of leaf number [formula X (described above)], rosette area (formula XV), plot coverage (formula XVI) and harvest index (formula IV) is calculated with the indicated formulas.

Relative growth rate of rosette area=Regression coefficient of rosette area along time course.  Formula XV:

Relative growth rate of plot coverage=Regression coefficient of plot coverage along time course.  Formula XVI

Seeds average weight—At the end of the experiment all seeds are collected. The seeds are scattered on a glass tray and a picture was taken. Using the digital analysis, the number of seeds in each sample is calculated.
Dry weight and seed yield—On about day 80 from sowing, the plants are harvested and left to dry at 30° C. in a drying chamber. The biomass and seed weight of each plot are measured and divided by the number of plants in each plot. Dry weight=total weight of the vegetative portion above ground (excluding roots) after drying at 30° C. in a drying chamber; Seed yield per plant=total seed weight per plant (gr). 1000 seed weight (the weight of 1000 seeds) (gr.).
The harvest index (HI) was calculated using Formula IV as described above.
Oil percentage in seeds—At the end of the experiment all seeds from each plot are collected. Seeds from 3 plots are mixed grounded and then mounted onto the extraction chamber. 210 ml of n-Hexane (Cat No. 080951 Biolab Ltd.) are used as the solvent. The extraction is performed for 30 hours at medium heat 50° C. Once the extraction has ended the n-Hexane was evaporated using the evaporator at 35° C. and vacuum conditions. The process is repeated twice. The information gained from the Soxhlet extractor (Soxhlet, F. Die gewichtsanalytische Bestimmung des Milchfettes, Polytechnisches J. (Dingler's) 1879, 232, 461) is used to create a calibration curve for the Low Resonance NMR. The content of oil of all seed samples is determined using the Low Resonance NMR (MARAN Ultra—Oxford Instrument) and its MultiQuant software package
Silique length analysis—On day 50 from sowing, 30 siliques from different plants in each plot are sampled in block A. The chosen siliques are green-yellow in color and are collected from the bottom parts of a grown plant's stem. A digital photograph is taken to determine silique's length.
Statistical analyses—To identify genes conferring significantly improved tolerance to abiotic stresses, the results obtained from the transgenic plants are compared to those obtained from control plants. To identify outperforming genes and constructs, results from the independent transformation events tested are analyzed separately. Data is analyzed using Student's t-test and results are considered significant if the p value was less than 0.1. The JMP statistics software package is used (Version 5.2.1, SAS Institute Inc., Cary, N.C., USA).
Example 16
Evaluation of Transgenic Arabidopsis NUE, Yield and Plant Growth Rate Under Low or Normal Nitrogen Fertilization in Greenhouse Assay
Assay 2: Nitrogen Use efficiency measured until bolting stage: plant biomass and plant growth rate at limited and optimal nitrogen concentration under greenhouse conditions—This assay follows the plant biomass formation and the rosette area growth of plants grown in the greenhouse at limiting and non-limiting nitrogen growth conditions. Transgenic Arabidopsis seeds were sown in agar media supplemented with 1⁄2 MS medium and a selection agent (Kanamycin). The T2 transgenic seedlings were then transplanted to 1.7 trays filled with peat and perlite in a 1:1 ratio. The trays were irrigated with a solution containing nitrogen limiting conditions, which were achieved by irrigating the plants with a solution containing 1.5 mM inorganic nitrogen in the form of KNO3, supplemented with 1 mM KH2PO4, 1 mM MgSO4, 3.6 mM KCl, 2 mM CaCl2 and microelements, while normal nitrogen levels were achieved by applying a solution of 6 mM inorganic nitrogen also in the form of KNO3 with 1 mM KH2PO4, 1 mM MgSO4, 2 mM CaCl2 and microelements. All plants were grown in the greenhouse until mature seeds. Plant biomass (the above ground tissue) was weight in directly after harvesting the rosette (plant fresh weight [FW]). Following plants were dried in an oven at 50° C. for 48 hours and weighted (plant dry weight [DW]).
Each construct was validated at its T2 generation. Transgenic plants transformed with a construct conformed by an empty vector carrying the 35S promoter and the selectable marker was used as control.
The plants were analyzed for their overall size, growth rate, fresh weight and dry matter. Transgenic plants performance was compared to control plants grown in parallel under the same conditions. Mock-transgenic plants expressing the uidA reporter gene (GUS-Intron) or with no gene at all, under the same promoter were used as control.
The experiment was planned in nested randomized plot distribution. For each gene of the invention three to five independent transformation events were analyzed from each construct.
Digital imaging—A laboratory image acquisition system, which consists of a digital reflex camera (Canon EOS 300D) attached with a 55 mm focal length lens (Canon EF-S series), mounted on a reproduction device (Kaiser RS), which includes 4 light units (4×150 Watts light bulb) was used for capturing images of plant samples.
The image capturing process was repeated every 2 days starting from day 1 after transplanting till day 15. Same camera, placed in a custom made iron mount, was used for capturing images of larger plants sawn in white tubs in an environmental controlled greenhouse. The tubs were square shape include 1.7 liter trays. During the capture process, the tubes were placed beneath the iron mount, while avoiding direct sun light and casting of shadows.
An image analysis system was used, which consists of a personal desktop computer (Intel P4 3.0 GHz processor) and a public domain program—ImageJ 1.39 [Java based image processing program which was developed at the U.S. National Institutes of Health and freely available on the internet at Hypertext Transfer Protocol://rsbweb (dot) nih (dot) gov/]. Images were captured in resolution of 10 Mega Pixels (3888×2592 pixels) and stored in a low compression JPEG (Joint Photographic Experts Group standard) format. Next, analyzed data was saved to text files and processed using the JMP statistical analysis software (SAS institute).
Leaf analysis—Using the digital analysis leaves data was calculated, including leaf number, rosette area, rosette diameter, leaf blade area.
Vegetative growth rate: the relative growth rate (RGR) of leaf number (Formula X, described above), rosette area (Formula XV described above) and plot coverage (Formula XVI, described above) are calculated using the indicated formulas.
Plant Fresh and Dry weight—On about day 80 from sowing, the plants were harvested and directly weight for the determination of the plant fresh weight (FW) and left to dry at 50° C. in a drying chamber for about 48 hours before weighting to determine plant dry weight (DW).
Statistical analyses—To identify genes conferring significantly improved tolerance to abiotic stresses, the results obtained from the transgenic plants were compared to those obtained from control plants. To identify outperforming genes and constructs, results from the independent transformation events tested were analyzed separately. Data was analyzed using Student's t-test and results are considered significant if the p value was less than 0.1. The JMP statistics software package was used (Version 5.2.1, SAS Institute Inc., Cary, N.C., USA).
Experimental Results:
The genes listed in Tables 78 and 79 improved plant NUE when grown at limiting nitrogen concentration levels. These genes produced larger plants with a larger photosynthetic area, biomass (fresh weight, dry weight, rosette diameter, rosette area and plot coverage) when grown under limiting nitrogen conditions. The genes were cloned under the regulation of a constitutive (At6669) and root preferred promoter (RootP). The evaluation of each gene was performed by testing the performance of different number of events. Event with p-value <0.1 was considered statistically significant







TABLE 78







Genes showing improved plant biomass production at limiting nitrogen growth conditions











Dry Weight [g]

Fresh Weight [g]
















Gene


P-
%
Gene


P-
%


Name
Event #
Ave.
Value
incr.
Name
Event #
Ave.
Value
incr.



















CONT.
—
0.139
—
0.0
CONT.
—
0.890
—
0.0


LNU100
14471.4
0.157
0.252
12.8
LNU104
25032.2
0.956
0.646
7.4


LNU104
25032.2
0.160
0.429
15.0
LNU106
14481.1
0.981
0.117
10.2


LNU104
25033.3
0.156
0.439
11.9
LNU106
14483.5
0.981
0.664
10.2


LNU106
14483.5
0.163
0.499
16.8
LNU114
25042.1
1.000
0.461
12.3


LNU106
14483.2
0.162
0.637
16.4
LNU155
14525.1
0.975
0.648
9.5


LNU106
14481.1
0.160
0.084
15.0
LNU213
24654.4
0.975
0.028
9.5


LNU106
14484.3
0.146
0.658
4.7
LNU218
24781.4
0.919
0.410
3.2


LNU114
25042.1
0.166
0.483
19.0
LNU23
25163.6
0.919
0.710
3.2


LNU155
14525.1
0.169
0.360
21.3
LNU23
25163.2
0.913
0.610
2.5


LNU213
24654.4
0.168
0.222
20.8
LNU28
25171.2
1.000
0.008
12.3


LNU218
24781.4
0.156
0.134
12.3
LNU4
25134.1
0.956
0.430
7.4


LNU23
25163.6
0.159
0.032
14.1
LNU40
24792.1
0.994
0.670
11.6


LNU23
25163.2
0.156
0.333
12.3
LNU40
24794.4
0.946
0.664
6.3


LNU23
25163.5
0.148
0.677
6.0
LNU46
14462.5
0.988
0.329
10.9


LNU28
25171.2
0.169
0.004
21.3
LNU46
14462.1
0.975
0.190
9.5


LNU28
25171.1
0.146
0.395
4.7
LNU48
24804.4
0.963
0.502
8.1


LNU4
25134.1
0.164
0.007
18.1
LNU63
24814.2
1.056
0.077
18.6


LNU4
25131.1
0.159
0.146
14.1
LNU63
24811.2
1.050
0.606
17.9


LNU4
25133.3
0.146
0.374
5.1
LNU7
25082.7
0.956
0.116
7.4


LNU40
24794.4
0.164
0.399
17.8
LNU8
25062.2
1.019
0.009
14.4


LNU40
24792.1
0.155
0.607
11.4
LNU94
24833.1
0.969
0.502
8.8


LNU40
24794.3
0.151
0.254
8.7
LNU94
24834.4
0.946
0.742
6.2


LNU40
24792.2
0.148
0.782
6.7
LNU96
25073.3
0.956
0.335
7.4


LNU46
14462.5
0.172
0.487
23.5
CONT.
—
0.761
—
0.0


LNU46
14464.4
0.148
0.461
6.5
LNU113
25631.3
0.900
0.458
18.3


LNU46
14462.1
0.146
0.699
5.1
LNU113
25631.7
0.894
0.196
17.5


LNU48
24804.4
0.159
0.209
14.6
LNU113
25631.9
0.875
0.032
15.0


LNU48
24801.4
0.146
0.584
4.7
LNU113
25631.4
0.863
0.232
13.4


LNU63
24811.2
0.184
0.571
32.5
LNU113
25631.1
0.813
0.149
6.8


LNU63
24814.2
0.168
0.222
20.8
LNU120
25463.3
1.119
0.016
47.1


LNU7
25082.7
0.142
0.765
2.0
LNU120
25465.3
0.856
0.204
12.6


LNU8
25062.2
0.170
0.276
22.2
LNU124
14501.1
0.813
0.475
6.8


LNU8
25061.2
0.159
0.721
14.6
LNU124
14502.1
0.794
0.388
4.4


LNU8
25063.1
0.149
0.503
7.4
LNU124
14504.5
0.788
0.439
3.5


LNU94
24833.1
0.161
0.205
15.9
LNU132
14102.6
1.013
0.141
33.1


LNU94
24834.4
0.161
0.651
15.9
LNU132
14102.7
0.906
0.345
19.2


LNU94
24833.3
0.147
0.413
5.7
LNU140
14112.6
0.931
0.000
22.4


LNU96
25073.3
0.163
0.341
17.3
LNU140
14111.6
0.844
0.139
10.9


LNU96
25071.2
0.149
0.707
7.4
LNU148
25685.6
0.906
0.174
19.2


LNU96
25073.4
0.147
0.602
5.6
LNU148
25685.2
0.881
0.057
15.9


CONT.
—
0.122
—
0.0
LNU148
25685.1
0.831
0.046
9.3


LNU113
25631.7
0.146
0.446
20.1
LNU148
25685.9
0.813
0.475
6.8


LNU113
25631.3
0.133
0.620
9.4
LNU287
24674.6
1.006
0.270
32.3


LNU113
25631.1
0.131
0.398
7.3
LNU287
24674.3
0.775
0.732
1.9


LNU120
25463.3
0.173
0.184
41.7
LNU37
14064.7
0.775
0.653
1.9


LNU120
25463.7
0.125
0.732
2.6
LNU5
14042.7
0.869
0.168
14.2


LNU132
14102.7
0.143
0.245
17.6
LNU5
14043.7
0.788
0.529
3.5


LNU132
14102.6
0.140
0.451
15.0
LNU72
24962.3
0.800
0.264
5.2


LNU140
14112.6
0.149
0.329
22.7
LNU74
25443.3
0.956
0.259
25.7


LNU148
25685.6
0.144
0.002
18.6
LNU82
24823.1
0.850
0.014
11.8


LNU148
25685.2
0.138
0.321
13.0
LNU84
25621.8
0.831
0.790
9.3


LNU148
25685.9
0.129
0.199
6.3
LNU84
25621.2
0.794
0.498
4.4


LNU148
25685.1
0.127
0.651
4.2
LNU87
24712.1
0.869
0.657
14.2


LNU287
24674.6
0.156
0.008
28.4
LNU87
24711.3
0.825
0.542
8.5


LNU74
25443.3
0.151
0.071
24.2
LNU87
24713.2
0.794
0.318
4.4


LNU74
25443.5
0.138
0.626
13.0
LNU87
24714.3
0.794
0.756
4.4


LNU74
25444.1
0.135
0.209
10.9
LNU98
25761.6
0.900
0.422
18.3


LNU74
25443.2
0.126
0.521
3.2
LNU98
25762.2
0.794
0.756
4.4


LNU82
24823.1
0.152
0.303
24.8
CONT.
—
0.974
—
0.0


LNU84
25621.8
0.146
0.652
19.6
LNU84
25621.2
1.031
0.280
5.9


LNU84
25621.2
0.128
0.540
4.7
CONT.
—
0.851
—
0.0


LNU87
24712.1
0.148
0.595
21.2
LNU117
25933.3
1.113
0.657
30.8


LNU87
24714.3
0.143
0.613
17.1
LNU117
25931.2
1.050
0.004
23.4


LNU87
24712.4
0.126
0.762
3.7
LNU117
25932.4
0.975
0.272
14.6


LNU87
24713.2
0.124
0.757
2.2
LNU122
25333.2
1.481
0.233
74.1


LNU98
25761.6
0.132
0.543
8.3
LNU122
25332.2
1.181
0.387
38.9


LNU98
25762.2
0.131
0.639
7.8
LNU122
25333.1
1.031
0.101
21.2


LNU98
25763.2
0.125
0.722
2.7
LNU125
25944.3
1.256
0.343
47.7


CONT.
—
0.129
—
0.0
LNU125
25941.4
1.244
0.381
46.2


LNU82
24823.1
0.144
0.134
11.9
LNU125
25941.2
1.081
0.443
27.1


LNU84
25621.2
0.149
0.002
15.3
LNU138
14074.5
1.488
0.001
74.9


LNU98
25763.2
0.133
0.619
3.2
LNU138
14071.5
1.425
0.157
67.5


CONT.
—
0.124
—
0.0
LNU138
14074.6
1.413
0.048
66.1


LNU117
25933.3
0.160
0.692
29.2
LNU138
14072.8
1.244
0.394
46.2


LNU117
25931.2
0.135
0.237
9.0
LNU138
14072.5
1.138
0.422
33.7


LNU122
25333.2
0.228
0.287
84.3
LNU180
24724.1
1.200
0.533
41.1


LNU122
25332.2
0.180
0.412
45.4
LNU180
24721.4
1.119
0.135
31.5


LNU122
25333.1
0.144
0.003
16.1
LNU180
24721.2
1.100
0.043
29.3


LNU125
25944.3
0.179
0.470
44.9
LNU180
24722.2
0.944
0.039
11.0


LNU125
25941.4
0.179
0.500
44.4
LNU180
24723.1
0.881
0.361
3.6


LNU125
25941.2
0.157
0.624
26.7
LNU220
25405.1
1.038
0.453
22.0


LNU138
14074.5
0.224
0.031
81.2
LNU220
25405.3
0.969
0.699
13.9


LNU138
14071.5
0.216
0.199
74.7
LNU220
25405.5
0.931
0.368
9.5


LNU138
14074.6
0.205
0.091
65.6
LNU230
25413.1
1.338
0.437
57.2


LNU138
14072.8
0.169
0.584
36.3
LNU230
25412.2
1.212
0.205
42.4


LNU138
14072.5
0.147
0.695
18.6
LNU230
25413.2
1.019
0.475
19.8


LNU180
24724.1
0.180
0.575
45.4
LNU230
25412.1
0.913
0.311
7.3


LNU180
24721.4
0.159
0.346
28.2
LNU234
25014.5
1.544
0.386
81.5


LNU180
24721.2
0.151
0.119
22.2
LNU234
25014.4
1.056
0.657
24.2


LNU220
25405.3
0.148
0.660
19.1
LNU234
25014.8
1.013
0.706
19.0


LNU220
25405.1
0.143
0.642
15.6
LNU25
14083.7
1.100
0.429
29.3


LNU230
25413.1
0.193
0.512
55.5
LNU25
14084.6
1.038
0.606
22.0


LNU230
25412.2
0.183
0.218
47.5
LNU25
14082.8
0.981
0.466
15.4


LNU230
25413.2
0.149
0.654
20.1
LNU254
25782.5
1.463
0.185
71.9


LNU230
25412.1
0.134
0.269
8.0
LNU254
25781.3
1.256
0.328
47.7


LNU234
25014.5
0.221
0.492
78.2
LNU254
25782.4
1.094
0.559
28.6


LNU234
25014.4
0.152
0.664
22.7
LNU254
25781.5
0.969
0.024
13.9


LNU234
25014.8
0.151
0.725
21.7
LNU263
25794.6
1.138
0.366
33.7


LNU25
14084.6
0.154
0.634
24.2
LNU263
25794.3
1.106
0.003
30.1


LNU25
14083.7
0.148
0.668
19.1
LNU263
25791.3
1.069
0.339
25.6


LNU254
25782.5
0.226
0.199
82.2
LNU263
25794.8
0.956
0.030
12.4


LNU254
25782.4
0.171
0.547
37.8
LNU263
25792.2
0.869
0.680
2.1


LNU254
25781.3
0.166
0.527
34.3
LNU267
25804.3
1.269
0.334
49.2


LNU254
25781.5
0.129
0.138
4.5
LNU267
25803.1
1.106
0.003
30.1


LNU263
25794.6
0.163
0.456
31.3
LNU271
25912.1
1.150
0.626
35.2


LNU263
25794.8
0.138
0.011
11.6
LNU271
25913.3
1.119
0.529
31.5


LNU263
25794.3
0.138
0.018
11.1
LNU278
25812.3
0.913
0.311
7.3


LNU263
25791.3
0.129
0.765
4.5
LNU278
25814.1
0.900
0.140
5.8


LNU267
25804.3
0.197
0.323
59.0
LNU278
25814.3
0.900
0.252
5.8


LNU267
25803.1
0.156
0.034
26.2
LNU278
25813.2
0.888
0.660
4.3


LNU267
25804.4
0.134
0.759
8.0
LNU36
25561.2
1.250
0.571
47.0


LNU271
25913.3
0.168
0.604
35.8
LNU36
25562.3
1.050
0.519
23.4


LNU271
25912.1
0.167
0.666
34.9
LNU36
25562.4
0.938
0.665
10.2


LNU278
25814.3
0.129
0.708
4.0
LNU36
25562.9
0.925
0.548
8.7


LNU278
25812.3
0.126
0.674
1.5
LNU43
14422.8
1.156
0.394
35.9


LNU36
25561.2
0.193
0.589
55.5
LNU43
14421.1
1.150
0.219
35.2


LNU36
25562.3
0.149
0.429
20.1
LNU43
14423.6
0.938
0.207
10.2


LNU36
25562.4
0.138
0.749
11.6
LNU45
25052.11
1.050
0.538
23.4


LNU43
14421.1
0.159
0.254
28.2
LNU45
25052.9
0.931
0.786
9.5


LNU43
14422.8
0.159
0.580
28.2
LNU45
25052.12
0.906
0.797
6.5


LNU67
25824.5
0.156
0.585
26.2
LNU67
25824.5
1.194
0.287
40.3


LNU67
25824.3
0.154
0.513
24.7
LNU67
25824.3
1.050
0.600
23.4


CONT.
—
0.179
—
0.0
LNU67
25821.5
0.900
0.252
5.8


LNU100
14474.2
0.222
0.512
24.2
CONT.
—
1.058
—
0.0


LNU104
25033.3
0.213
0.558
19.3
LNU100
14474.2
1.325
0.357
25.2


LNU106
14483.2
0.232
0.720
29.8
LNU100
14473.3
1.269
0.718
19.9


LNU114
25041.2
0.215
0.661
20.4
LNU100
14472.1
1.144
0.663
8.1


LNU117
25932.4
0.363
0.306
103.3
LNU104
25033.3
1.288
0.462
21.7


LNU180
24723.1
0.341
0.543
90.7
LNU106
14483.2
1.369
0.687
29.4


LNU218
24781.2
0.278
0.651
55.7
LNU114
25041.2
1.206
0.671
14.0


LNU218
24781.1
0.227
0.359
27.0
LNU117
25932.4
1.931
0.268
82.5


LNU254
25781.3
0.333
0.021
86.1
LNU180
24723.1
1.844
0.530
74.3


LNU254
25782.5
0.217
0.285
21.4
LNU218
24781.2
1.450
0.684
37.1


LNU4
25133.3
0.256
0.106
43.4
LNU218
24781.1
1.406
0.410
32.9


LNU4
25134.2
0.193
0.707
8.1
LNU254
25781.3
1.750
0.038
65.4


LNU40
24794.3
0.259
0.557
45.2
LNU254
25782.5
1.181
0.425
11.7


LNU40
24793.1
0.254
0.522
42.4
LNU4
25133.3
1.400
0.093
32.3


LNU63
24814.7
0.333
0.446
86.1
LNU4
25134.3
1.206
0.509
14.0


LNU63
24814.2
0.283
0.221
58.5
LNU4
25134.2
1.163
0.479
9.9


LNU63
24812.3
0.256
0.305
43.4
LNU40
24794.3
1.544
0.515
45.9


LNU7
25082.2
0.197
0.697
10.2
LNU40
24793.1
1.431
0.486
35.3


LNU7
25083.3
0.189
0.753
5.7
LNU40
24792.1
1.231
0.695
16.4


LNU8
25063.6
0.193
0.669
7.8
LNU48
24803.2
1.144
0.774
8.1


CONT.
—
0.106
—
0.0
LNU48
24802.2
1.139
0.581
7.7


LNU122
25332.2
0.119
0.161
11.8
LNU63
24814.7
1.813
0.453
71.3


LNU122
25333.2
0.113
0.223
5.9
LNU63
24814.2
1.613
0.140
52.4


LNU125
25941.4
0.126
0.010
18.2
LNU63
24812.3
1.494
0.216
41.2


LNU125
25943.2
0.121
0.009
13.5
LNU7
25082.2
1.206
0.354
14.0


LNU138
14072.5
0.114
0.426
7.6
LNU7
25083.3
1.169
0.454
10.5


LNU178
14612.1
0.127
0.001
19.4
LNU7
25083.1
1.144
0.696
8.1


LNU178
14614.5
0.124
0.302
16.5
LNU8
25063.6
1.231
0.319
16.4


LNU220
25405.1
0.116
0.533
8.8
CONT.
—
0.690
—
0.0


LNU220
25405.5
0.114
0.260
7.6
LNU122
25332.2
0.775
0.505
12.4


LNU220
25405.6
0.108
0.798
1.2
LNU125
25941.4
0.775
0.586
12.4


LNU234
25014.6
0.128
0.076
20.6
LNU178
14612.1
0.838
0.235
21.4


LNU236
25425.4
0.134
0.302
25.9
LNU178
14614.5
0.781
0.147
13.3


LNU236
25424.2
0.118
0.690
10.6
LNU220
25405.1
0.806
0.468
16.9


LNU236
25423.3
0.116
0.436
8.8
LNU220
25405.5
0.756
0.004
9.6


LNU25
14082.8
0.119
0.185
12.4
LNU234
25014.6
0.763
0.443
10.5


LNU271
25911.4
0.124
0.033
16.5
LNU236
25425.4
0.838
0.373
21.4


LNU271
25913.3
0.109
0.633
2.9
LNU236
25424.2
0.781
0.546
13.3


LNU278
25814.1
0.111
0.707
4.7
LNU236
25423.3
0.731
0.508
6.0


LNU43
14423.6
0.118
0.062
11.2
LNU25
14082.8
0.900
0.066
30.5


LNU43
14423.7
0.111
0.511
4.1
LNU271
25911.4
0.738
0.065
6.9


LNU45
25052.12
0.123
0.467
15.3
LNU271
25913.2
0.725
0.360
5.1


LNU45
25053.4
0.121
0.004
14.1
LNU278
25814.1
0.738
0.507
6.9


LNU45
25052.11
0.118
0.341
10.6
LNU278
25814.3
0.719
0.334
4.2


LNU67
25821.5
0.150
0.379
41.2
LNU43
14423.6
0.775
0.007
12.4


LNU67
25823.5
0.126
0.010
18.2
LNU43
14422.9
0.731
0.197
6.0


LNU67
25824.3
0.118
0.045
10.6
LNU43
14423.7
0.700
0.767
1.5


LNU9
25001.2
0.122
0.205
14.7
LNU45
25052.12
0.813
0.431
17.8


LNU9
25003.1
0.118
0.341
10.6
LNU45
25053.4
0.813
0.139
17.8


LNU9
25001.3
0.110
0.710
3.5
LNU67
25821.5
0.994
0.378
44.1


CONT.
—
0.110
—
0.0
LNU67
25821.4
0.831
0.000
20.5


LNU157
24982.8
0.130
0.146
18.6
LNU67
25823.5
0.775
0.099
12.4


LNU157
24982.4
0.123
0.285
11.8
LNU67
25824.3
0.763
0.014
10.5


LNU157
24983.3
0.120
0.318
9.5
LNU67
25824.5
0.738
0.507
6.9


LNU168
24754.2
0.116
0.617
5.5
LNU9
25003.1
0.819
0.238
18.7


LNU173
25451.5
0.164
0.027
49.4
LNU9
25001.7
0.725
0.510
5.1


LNU173
25451.11
0.155
0.001
41.4
LNU9
25001.2
0.706
0.562
2.4


LNU173
25451.1
0.140
0.140
27.8
CONT.
—
0.728
—
0.0


LNU173
25451.2
0.139
0.337
27.2
LNU157
24982.8
0.856
0.199
17.7


LNU173
25451.12
0.131
0.235
19.2
LNU157
24982.4
0.794
0.422
9.1


LNU178
14612.1
0.126
0.575
14.6
LNU168
24754.2
0.856
0.199
17.7


LNU178
14611.5
0.122
0.233
11.2
LNU173
25451.5
1.069
0.001
46.9


LNU178
14611.4
0.121
0.239
10.6
LNU173
25451.11
1.019
0.002
40.0


LNU178
14611.1
0.120
0.419
9.5
LNU173
25451.2
0.950
0.073
30.5


LNU184
25393.3
0.163
0.236
48.3
LNU173
25451.1
0.938
0.047
28.8


LNU184
25393.1
0.148
0.007
34.6
LNU173
25451.12
0.888
0.399
21.9


LNU184
25393.2
0.147
0.109
34.0
LNU178
14611.4
0.913
0.019
25.4


LNU184
25395.1
0.132
0.075
20.3
LNU178
14612.1
0.869
0.525
19.4


LNU184
25394.3
0.121
0.239
10.6
LNU178
14611.5
0.850
0.092
16.8


LNU20
24933.4
0.154
0.034
40.9
LNU178
14611.1
0.800
0.286
9.9


LNU20
24932.4
0.143
0.431
30.0
LNU184
25393.1
0.994
0.013
36.5


LNU20
24934.1
0.142
0.019
29.5
LNU184
25393.3
0.994
0.100
36.5


LNU20
24933.1
0.120
0.617
9.5
LNU184
25393.2
0.975
0.027
34.0


LNU230
25413.2
0.162
0.022
47.7
LNU184
25395.1
0.888
0.103
21.9


LNU230
25413.1
0.144
0.005
31.7
LNU184
25394.3
0.856
0.077
17.7


LNU230
25415.1
0.144
0.007
31.2
LNU20
24933.4
1.088
0.002
49.4


LNU230
25412.2
0.126
0.148
15.2
LNU20
24934.1
0.950
0.010
30.5


LNU230
25412.1
0.119
0.510
8.4
LNU20
24932.4
0.851
0.585
16.9


LNU236
25425.4
0.153
0.002
39.2
LNU20
24933.1
0.825
0.280
13.4


LNU236
25424.2
0.124
0.160
12.9
LNU230
25413.2
1.131
0.000
55.4


LNU236
25422.4
0.119
0.502
8.9
LNU230
25413.1
0.969
0.043
33.1


LNU236
25423.3
0.119
0.343
8.4
LNU230
25415.1
0.925
0.014
27.1


LNU236
25425.3
0.116
0.546
5.5
LNU230
25412.2
0.913
0.024
25.4


LNU24
24971.3
0.158
0.001
44.3
LNU230
25412.1
0.800
0.315
9.9


LNU24
24971.2
0.143
0.029
30.6
LNU236
25425.4
1.031
0.001
41.7


LNU24
24971.4
0.136
0.141
24.3
LNU236
25422.4
0.863
0.271
18.5


LNU24
24974.2
0.123
0.205
11.8
LNU236
25424.2
0.850
0.092
16.8


LNU24
24972.1
0.116
0.533
5.5
LNU236
25423.3
0.831
0.141
14.2


LNU263
25794.8
0.156
0.050
42.0
LNU236
25425.3
0.800
0.315
9.9


LNU263
25794.6
0.146
0.047
33.5
LNU24
24971.3
0.994
0.003
36.5


LNU263
25794.3
0.141
0.162
28.9
LNU24
24971.2
0.963
0.104
32.3


LNU263
25791.3
0.138
0.021
26.0
LNU24
24971.4
0.869
0.282
19.4


LNU263
25792.2
0.129
0.524
18.1
LNU24
24974.2
0.863
0.067
18.5


LNU276
25433.2
0.144
0.006
31.2
LNU24
24972.1
0.831
0.141
14.2


LNU276
25433.1
0.133
0.362
20.9
LNU263
25794.8
0.988
0.004
35.7


LNU276
25433.3
0.131
0.045
19.8
LNU263
25794.6
0.938
0.011
28.8


LNU276
25433.5
0.124
0.685
12.9
LNU263
25794.3
0.913
0.164
25.4


LNU276
25431.1
0.117
0.611
6.7
LNU263
25792.2
0.900
0.186
23.7


LNU279
25481.5
0.165
0.000
50.6
LNU263
25791.3
0.888
0.043
21.9


LNU279
25481.3
0.163
0.000
48.3
LNU276
25433.2
0.944
0.100
29.7


LNU279
25481.4
0.146
0.022
32.9
LNU276
25433.1
0.881
0.139
21.1


LNU279
25481.2
0.144
0.237
31.7
LNU276
25433.3
0.838
0.230
15.1


LNU279
25484.3
0.132
0.137
20.3
LNU276
25433.5
0.831
0.601
14.2


LNU36
25561.2
0.188
0.000
71.1
LNU276
25431.1
0.819
0.231
12.5


LNU36
25562.3
0.183
0.214
66.5
LNU279
25481.5
1.063
0.001
46.0


LNU36
25562.7
0.131
0.057
19.2
LNU279
25481.2
0.981
0.228
34.8


LNU36
25562.9
0.123
0.367
11.8
LNU279
25484.3
0.981
0.016
34.8


LNU53
25674.1
0.136
0.022
23.8
LNU279
25481.4
0.975
0.056
34.0


LNU53
25674.3
0.119
0.502
8.9
LNU279
25481.3
0.931
0.333
28.0


LNU53
25674.6
0.118
0.383
7.8
LNU36
25562.3
1.325
0.249
82.1


LNU53
25674.2
0.114
0.659
3.8
LNU36
25561.2
1.175
0.000
61.5


LNU56
24693.1
0.164
0.042
49.4
LNU36
25562.9
0.938
0.013
28.8


LNU56
24691.2
0.151
0.006
37.5
LNU36
25562.7
0.913
0.019
25.4


LNU56
24693.2
0.137
0.187
24.9
LNU53
25674.1
0.906
0.058
24.5


LNU56
24694.2
0.133
0.032
21.5
LNU53
25674.6
0.831
0.178
14.2


LNU56
24694.1
0.133
0.186
20.9
LNU53
25674.3
0.819
0.545
12.5


LNU73
25755.1
0.142
0.008
29.5
LNU53
25674.2
0.781
0.423
7.3


LNU73
25751.9
0.138
0.129
25.5
LNU56
24693.1
1.125
0.000
54.6


LNU73
25751.1
0.133
0.063
21.5
LNU56
24693.2
0.988
0.023
35.7


LNU73
25751.8
0.118
0.628
7.2
LNU56
24691.2
0.938
0.010
28.8


LNU73
25754.2
0.116
0.488
6.1
LNU56
24694.1
0.938
0.047
28.8


LNU9
25001.7
0.163
0.236
48.3
LNU56
24694.2
0.925
0.014
27.1


LNU9
25001.1
0.137
0.020
24.9
LNU73
25751.9
0.963
0.007
32.3


LNU9
25001.3
0.119
0.314
8.9
LNU73
25755.1
0.894
0.225
22.8


LNU9
25001.2
0.116
0.655
6.1
LNU73
25751.1
0.881
0.041
21.1


CONT.
—
0.112
—
0.0
LNU73
25751.8
0.806
0.557
10.8


LNU131
14005.5
0.150
0.000
34.2
LNU73
25754.2
0.763
0.650
4.8


LNU131
14002.15
0.138
0.186
23.0
LNU9
25001.7
1.031
0.213
41.7


LNU131
14005.2
0.133
0.015
18.5
LNU9
25001.1
0.981
0.004
34.8


LNU131
14002.12
0.119
0.302
6.8
LNU9
25001.3
0.863
0.079
18.5


LNU135
26204.2
0.156
0.103
39.2
LNU9
25001.2
0.794
0.343
9.1


LNU135
26203.4
0.137
0.363
22.4
CONT.
—
0.865
—
0.0


LNU135
26203.6
0.128
0.021
14.6
LNU131
14002.15
0.981
0.019
13.4


LNU135
26203.1
0.125
0.074
11.8
LNU131
14005.5
0.931
0.259
7.6


LNU135
26204.4
0.118
0.315
5.1
LNU131
14005.2
0.925
0.193
6.9


LNU161
14552.7
0.124
0.673
10.7
LNU135
26204.2
1.063
0.003
22.8


LNU161
14553.4
0.123
0.207
9.6
LNU135
26203.4
0.956
0.542
10.5


LNU161
14553.6
0.122
0.343
9.0
LNU135
26203.6
0.950
0.202
9.8


LNU161
14552.9
0.121
0.457
7.9
LNU135
26203.1
0.906
0.471
4.7


LNU173
25451.5
0.119
0.455
6.2
LNU161
14552.9
0.956
0.220
10.5


LNU173
25451.11
0.115
0.753
2.9
LNU161
14552.7
0.925
0.690
6.9


LNU181
25771.6
0.116
0.522
3.4
LNU161
14553.4
0.881
0.722
1.9


LNU181
25771.2
0.115
0.516
2.9
LNU184
25395.1
1.031
0.181
19.2


LNU184
25395.1
0.153
0.000
36.4
LNU184
25393.3
0.975
0.054
12.7


LNU184
25394.3
0.134
0.104
20.2
LNU184
25394.1
0.931
0.561
7.6


LNU184
25394.1
0.131
0.039
16.9
LNU184
25394.3
0.913
0.594
5.5


LNU184
25393.3
0.127
0.007
13.5
LNU224
25874.4
1.063
0.166
22.8


LNU224
25874.4
0.141
0.250
26.4
LNU224
25872.2
1.013
0.051
17.0


LNU224
25871.3
0.136
0.001
21.9
LNU224
25871.3
0.956
0.220
10.5


LNU224
25872.2
0.136
0.068
21.9
LNU224
25872.3
0.904
0.500
4.5


LNU224
25872.3
0.133
0.063
19.0
LNU246
25744.2
1.044
0.312
20.6


LNU224
25874.1
0.132
0.076
18.0
LNU246
25743.1
0.963
0.049
11.2


LNU246
25744.2
0.140
0.232
25.2
LNU246
25743.2
0.963
0.082
11.2


LNU246
25743.1
0.135
0.211
20.8
LNU250
25592.1
1.000
0.135
15.6


LNU246
25743.2
0.131
0.143
16.9
LNU250
25592.2
0.963
0.049
11.2


LNU250
25592.2
0.149
0.195
33.1
LNU276
25433.5
1.163
0.004
34.4


LNU250
25592.1
0.132
0.008
18.0
LNU276
25433.6
0.931
0.704
7.6


LNU260
26404.8
0.126
0.021
12.9
LNU276
25433.3
0.919
0.353
6.2


LNU260
26404.7
0.125
0.585
11.8
LNU276
25433.1
0.894
0.670
3.3


LNU260
26403.1
0.121
0.239
7.9
LNU279
25481.2
1.106
0.146
27.9


LNU260
26403.2
0.115
0.566
2.9
LNU279
25481.3
1.100
0.038
27.1


LNU276
25433.5
0.146
0.113
30.3
LNU279
25481.4
1.094
0.023
26.4


LNU276
25433.6
0.133
0.212
19.1
LNU279
25484.3
1.088
0.000
25.7


LNU276
25433.1
0.122
0.407
9.0
LNU279
25481.5
1.025
0.095
18.5


LNU276
25433.3
0.119
0.164
6.8
LNU3
26124.1
1.106
0.146
27.9


LNU276
25431.1
0.113
0.800
1.2
LNU3
26122.2
1.100
0.082
27.1


LNU279
25481.3
0.151
0.320
35.3
LNU3
26123.6
0.994
0.011
14.9


LNU279
25484.3
0.149
0.000
33.6
LNU3
26123.5
0.894
0.791
3.3


LNU279
25481.4
0.148
0.120
31.9
LNU33
25553.2
1.010
0.103
16.7


LNU279
25481.2
0.134
0.104
20.2
LNU33
25552.2
0.906
0.404
4.7


LNU279
25481.5
0.129
0.268
15.7
CONT.
—
0.723
—
0.0


LNU3
26122.2
0.147
0.000
31.4
LNU119
26142.8.
0.988
0.051
36.7


LNU3
26124.1
0.141
0.344
25.8
LNU119
26144.2.
0.913
0.000
26.3


LNU3
26123.6
0.126
0.230
12.4
LNU119
26142.5.
0.869
0.035
20.2


LNU3
26124.3
0.121
0.067
8.5
LNU119
26141.1.
0.819
0.563
13.3


LNU3
26123.5
0.121
0.459
8.5
LNU130
24912.7.
1.119
0.093
54.8


LNU33
25553.2
0.140
0.293
25.6
LNU130
24911.7.
1.113
0.000
54.0


LNU33
25552.2
0.138
0.161
23.6
LNU130
24913.5.
0.931
0.052
28.9


LNU33
25551.1
0.123
0.571
10.1
LNU130
24913.6.
0.913
0.000
26.3


CONT.
—
0.072
—
0.0
LNU130
24914.5.
0.863
0.380
19.4


LNU119
26142.8.
0.094
0.155
30.0
LNU136
14511.10.
1.213
0.000
67.8


LNU119
26144.2.
0.088
0.179
21.3
LNU136
14514.8.
1.163
0.000
60.9


LNU119
26141.1.
0.081
0.474
12.7
LNU136
14515.5.
1.144
0.086
58.3


LNU130
24911.7.
0.118
0.000
63.8
LNU136
14513.6.
1.007
0.171
39.4


LNU130
24912.7.
0.114
0.189
58.6
LNU136
14515.1.
1.006
0.000
39.3


LNU130
24913.6.
0.098
0.003
36.0
LNU142
27541.1.
1.194
0.001
65.2


LNU130
24914.5.
0.094
0.405
30.8
LNU142
27545.1.
0.969
0.000
34.1


LNU130
24913.5.
0.091
0.129
26.5
LNU142
27546.1.
0.919
0.015
27.2


LNU136
14511.10.
0.131
0.018
82.0
LNU142
27546.2.
0.894
0.338
23.7


LNU136
14515.5.
0.124
0.014
72.4
LNU142
27541.2.
0.816
0.322
13.0


LNU136
14514.8.
0.114
0.000
58.6
LNU149
26175.3.
1.309
0.001
81.2


LNU136
14515.1.
0.102
0.000
41.2
LNU149
26175.1.
1.094
0.016
51.4


LNU136
14513.6.
0.099
0.068
37.2
LNU149
26175.7.
0.855
0.575
18.4


LNU142
27541.1.
0.126
0.003
74.2
LNU149
26174.4.
0.838
0.699
15.9


LNU142
27546.2.
0.101
0.007
40.4
LNU149
26174.6.
0.813
0.655
12.5


LNU142
27546.1.
0.095
0.188
31.7
LNU15
14123.11.
1.121
0.146
55.1


LNU142
27545.1.
0.094
0.056
30.0
LNU15
14123.13.
0.994
0.410
37.5


LNU142
27541.2.
0.080
0.450
10.3
LNU15
14122.8.
0.988
0.387
36.7


LNU149
26175.3.
0.135
0.098
86.9
LNU15
14122.9.
0.969
0.352
34.1


LNU149
26175.1.
0.116
0.002
61.2
LNU15
14124.12.
0.844
0.521
16.8


LNU149
26174.4.
0.094
0.352
30.8
LNU185
26475.1.
1.131
0.381
56.6


LNU149
26175.7.
0.093
0.203
28.6
LNU185
26474.1.
0.956
0.368
32.4


LNU149
26174.6.
0.081
0.651
12.7
LNU185
26473.1.
0.950
0.413
31.5


LNU15
14123.11.
0.118
0.012
63.2
LNU185
26474.2.
0.919
0.448
27.2


LNU15
14122.8.
0.103
0.403
43.0
LNU212
25834.4.
1.294
0.095
79.1


LNU15
14122.9.
0.103
0.401
42.1
LNU212
25834.5.
1.250
0.131
73.0


LNU15
14124.12.
0.092
0.246
27.4
LNU212
25834.1.
1.081
0.208
49.7


LNU15
14123.13.
0.091
0.183
26.5
LNU212
25833.2.
1.056
0.173
46.2


LNU185
26475.1.
0.120
0.272
66.4
LNU212
25832.1.
1.019
0.026
41.0


LNU185
26474.2.
0.108
0.374
49.9
LNU216
25985.4.
1.313
0.000
81.7


LNU185
26474.1.
0.094
0.429
30.8
LNU216
25982.1.
1.150
0.046
59.2


LNU185
26473.1.
0.093
0.297
28.2
LNU216
25984.6.
0.938
0.027
29.8


LNU212
25834.5.
0.134
0.024
86.3
LNU216
25982.2.
0.863
0.001
19.4


LNU212
25834.4.
0.126
0.092
74.2
LNU216
25984.1.
0.844
0.602
16.8


LNU212
25833.2.
0.121
0.082
67.2
LNU228
26222.4.
1.275
0.000
76.5


LNU212
25834.1.
0.116
0.118
60.3
LNU228
26222.1.
1.181
0.100
63.5


LNU212
25832.1.
0.103
0.006
42.1
LNU228
26224.7.
1.019
0.004
41.0


LNU216
25985.4.
0.119
0.097
64.6
LNU228
26225.2.
0.856
0.615
18.5


LNU216
25982.1.
0.109
0.103
50.8
LNU228
26224.6.
0.775
0.471
7.3


LNU216
25984.6.
0.084
0.015
16.1
LNU229
26112.3.
1.131
0.013
56.6


LNU216
25984.1.
0.083
0.586
14.4
LNU229
26111.5.
0.838
0.607
15.9


LNU216
25982.2.
0.076
0.437
4.9
LNU241
26232.4.
0.800
0.751
10.7


LNU228
26222.4.
0.133
0.000
83.7
LNU274
26261.3.
0.794
0.305
9.9


LNU228
26222.1.
0.115
0.215
59.4
LNU280
26164.4.
0.843
0.137
16.7


LNU228
26224.7.
0.100
0.215
38.6
LNU280
26162.1.
0.800
0.765
10.7


LNU228
26225.2.
0.091
0.511
26.5
LNU55
26013.9.
0.756
0.491
4.7


LNU228
26224.6.
0.086
0.448
18.7
LNU55
26015.1.
0.750
0.396
3.8


LNU229
26112.3.
0.113
0.117
56.0
LNU81
26034.2.
0.806
0.530
11.6


LNU229
26111.5.
0.086
0.658
19.6
CONT.
—
0.625
—
0.0


LNU241
26232.4.
0.078
0.767
8.3
LNU119
26141.1.
0.769
0.001
23.0


LNU274
26261.3.
0.084
0.101
16.1
LNU119
26142.8.
0.719
0.318
15.0


LNU274
26263.2.
0.074
0.613
3.0
LNU119
26142.5.
0.688
0.554
10.0


LNU280
26162.1.
0.085
0.545
17.9
LNU119
26144.1.
0.681
0.053
9.0


LNU280
26164.4.
0.083
0.027
14.5
LNU119
26144.2.
0.650
0.708
4.0


LNU81
26034.2.
0.083
0.301
14.4
LNU130
24913.5.
0.856
0.067
37.0


LNU81
26034.3.
0.075
0.477
4.0
LNU130
24911.7.
0.794
0.446
27.0


CONT.
—
0.084
—
0.0
LNU130
24912.7.
0.719
0.388
15.0


LNU119
26142.8.
0.097
0.259
15.4
LNU130
24913.6.
0.681
0.053
9.0


LNU119
26142.5.
0.093
0.583
10.2
LNU136
14514.8.
0.713
0.011
14.0


LNU119
26141.1.
0.092
0.136
9.4
LNU136
14515.1.
0.694
0.492
11.0


LNU130
24913.5.
0.104
0.006
23.6
LNU136
14515.5.
0.669
0.204
7.0


LNU130
24911.7.
0.094
0.675
11.7
LNU142
27546.1.
0.681
0.742
9.0


LNU130
24913.6.
0.094
0.448
11.7
LNU142
27545.1.
0.656
0.678
5.0


LNU130
24912.7.
0.092
0.680
9.4
LNU142
27546.2.
0.644
0.798
3.0


LNU149
26174.6.
0.102
0.307
21.4
LNU149
26174.6.
0.869
0.147
39.0


LNU149
26175.3.
0.096
0.416
14.6
LNU149
26175.3.
0.844
0.025
35.0


LNU149
26174.7.
0.089
0.592
5.7
LNU149
26174.7.
0.781
0.005
25.0


LNU15
14122.9.
0.093
0.441
10.2
LNU149
26174.8.
0.669
0.638
7.0


LNU15
14123.13.
0.087
0.788
3.5
LNU149
26175.1.
0.656
0.729
5.0


LNU185
26475.1.
0.093
0.213
10.2
LNU15
14122.9.
0.825
0.160
32.0


LNU212
25834.1.
0.128
0.278
51.9
LNU15
14122.8.
0.750
0.605
20.0


LNU212
25834.5.
0.119
0.326
42.2
LNU15
14123.11.
0.694
0.026
11.0


LNU212
25833.2.
0.106
0.326
25.8
LNU15
14123.13.
0.650
0.511
4.0


LNU212
25833.1.
0.101
0.381
20.6
LNU185
26475.1.
0.756
0.223
21.0


LNU212
25834.4.
0.096
0.410
13.9
LNU185
26474.1.
0.706
0.364
13.0


LNU216
25985.4.
0.131
0.110
56.3
LNU185
26474.2.
0.669
0.204
7.0


LNU216
25984.1.
0.123
0.002
45.9
LNU212
25834.1.
1.150
0.214
84.0


LNU216
25984.6.
0.109
0.336
30.3
LNU212
25834.5.
0.975
0.310
56.0


LNU216
25982.1.
0.102
0.218
21.3
LNU212
25833.2.
0.894
0.282
43.0


LNU216
25982.2.
0.097
0.037
15.4
LNU212
25834.4.
0.888
0.000
42.0


LNU228
26222.4.
0.138
0.102
64.5
LNU212
25833.1.
0.844
0.206
35.0


LNU228
26225.2.
0.138
0.000
63.8
LNU216
25985.4.
1.325
0.006
112.0


LNU228
26222.1.
0.136
0.067
61.5
LNU216
25984.1.
1.056
0.180
69.0


LNU228
26224.7.
0.133
0.000
57.9
LNU216
25984.6.
0.963
0.320
54.0


LNU228
26224.6.
0.132
0.097
57.1
LNU216
25982.2.
0.794
0.106
27.0


LNU229
26112.4.
0.139
0.032
65.3
LNU216
25982.1.
0.781
0.239
25.0


LNU229
26111.7.
0.136
0.017
62.3
LNU228
26224.7.
1.302
0.001
108.3


LNU229
26112.3.
0.123
0.058
45.9
LNU228
26222.4.
1.300
0.145
108.0


LNU229
26111.5.
0.121
0.007
43.7
LNU228
26224.6.
1.231
0.127
97.0


LNU241
26234.1.
0.119
0.069
41.4
LNU228
26222.1.
1.219
0.000
95.0


LNU241
26233.2.
0.104
0.114
23.6
LNU228
26225.2.
1.038
0.092
66.0


LNU241
26232.4.
0.103
0.007
22.8
LNU229
26111.7.
1.219
0.016
95.0


LNU241
26232.1.
0.099
0.080
18.4
LNU229
26111.5.
1.169
0.000
87.0


LNU241
26233.3.
0.094
0.322
11.7
LNU229
26112.4.
1.169
0.038
87.0


LNU253
26241.1.
0.129
0.000
54.1
LNU229
26112.3.
1.150
0.001
84.0


LNU253
26245.1.
0.125
0.000
48.9
LNU229
26114.1.
0.763
0.392
22.0


LNU253
26242.1.
0.124
0.110
47.4
LNU241
26234.1.
1.150
0.029
84.0


LNU253
26243.3.
0.105
0.004
25.1
LNU241
26232.4.
0.863
0.131
38.0


LNU253
26244.2.
0.103
0.329
22.8
LNU241
26233.3.
0.831
0.000
33.0


LNU274
26261.3.
0.126
0.575
50.4
LNU241
26232.1.
0.794
0.042
27.0


LNU274
26262.2.
0.126
0.050
50.4
LNU241
26233.2.
0.794
0.000
27.0


LNU274
26264.2.
0.121
0.000
43.7
LNU253
26245.1.
1.150
0.010
84.0


LNU274
26263.2.
0.116
0.033
37.7
LNU253
26242.1.
1.081
0.093
73.0


LNU274
26265.1.
0.111
0.063
32.5
LNU253
26241.1.
1.056
0.026
69.0


LNU277
25844.3.
0.116
0.412
37.7
LNU253
26243.3.
1.013
0.000
62.0


LNU277
25842.3.
0.115
0.001
37.0
LNU253
26244.2.
0.944
0.138
51.0


LNU277
25844.4.
0.105
0.102
25.1
LNU274
26263.2.
1.156
0.161
85.0


LNU277
25841.3.
0.097
0.497
15.4
LNU274
26262.2.
1.125
0.130
80.0


LNU277
25845.1.
0.091
0.696
8.3
LNU274
26261.3.
1.025
0.537
64.0


LNU280
26164.3.
0.131
0.132
56.3
LNU274
26264.2.
0.994
0.000
59.0


LNU280
26162.1.
0.126
0.227
49.6
LNU274
26265.1.
0.994
0.230
59.0


LNU280
26162.7.
0.111
0.493
32.1
LNU277
25842.3.
0.925
0.004
48.0


LNU280
26164.4.
0.098
0.223
17.0
LNU277
25844.3.
0.919
0.388
47.0


LNU280
26164.2.
0.098
0.332
16.1
LNU277
25841.3.
0.825
0.110
32.0


LNU55
26013.9.
0.099
0.467
17.6
LNU277
25844.4.
0.819
0.234
31.0


LNU81
26034.3.
0.098
0.141
16.1
LNU277
25845.1.
0.750
0.556
20.0







LNU280
26164.3.
1.119
0.189
79.0







LNU280
26162.1.
1.038
0.316
66.0







LNU280
26162.7.
0.953
0.360
52.4







LNU280
26164.4.
0.928
0.346
48.4







LNU280
26164.2.
0.800
0.356
28.0







LNU55
26013.9.
0.806
0.363
29.0







LNU55
26013.3.
0.731
0.565
17.0







LNU81
26034.3.
0.756
0.010
21.0







LNU81
26034.2.
0.681
0.053
9.0





Table 78.


“CONT.”—Control;


“Ave.”—Average;


“% Incr.” = % increment.













TABLE 79







Genes showing improved plant biomass production at limiting nitrogen growth conditions













Rosette

Rosette Area

Plot Coverage



Diameter [cm]

[cm2]

[%]





















Gene


P-
%
Gene


P-
%
Gene


P-
%


Name
Event #
Ave.
Value
incr.
Name
Event #
Ave.
Value
incr.
Name
Event #
Ave.
Value
incr.
























CONT.
—
3.606
—
0
CONT.
—
4.630
—
0
CONT.
—
36.6
—
0


LNU100
14472.2
4.256
0.154
18
LNU100
14472.2
6.287
0.060
36
LNU100
14472.2
50.3
0.050
37


LNU100
14471.4
4.115
0.102
14
LNU100
14471.4
5.556
0.016
20
LNU100
14471.4
44.5
0.012
21


LNU100
14474.3
3.819
0.148
6
LNU100
14473.3
4.782
0.448
3
LNU100
14473.3
38.3
0.339
5


LNU100
14473.3
3.744
0.244
4
LNU100
14474.3
4.748
0.505
3
LNU100
14474.3
38.0
0.375
4


LNU104
25033.3
4.546
0.000
26
LNU104
25033.3
6.633
0.000
43
LNU104
25033.3
53.1
0.000
45


LNU104
25032.2
4.232
0.000
17
LNU104
25032.2
6.169
0.166
33
LNU104
25032.2
49.4
0.154
35


LNU104
25032.1
4.113
0.187
14
LNU104
25032.1
5.559
0.115
20
LNU104
25032.1
44.5
0.097
22


LNU104
25033.1
3.852
0.005
7
LNU104
25033.1
5.070
0.158
10
LNU104
25033.1
40.6
0.126
11


LNU106
14481.1
4.267
0.237
18
LNU106
14481.1
6.059
0.128
31
LNU106
14481.1
48.5
0.115
32


LNU106
14483.5
4.070
0.260
13
LNU106
14483.5
5.508
0.264
19
LNU106
14483.5
44.1
0.243
20


LNU106
14484.3
3.952
0.004
10
LNU106
14483.2
5.269
0.017
14
LNU106
14483.2
42.2
0.013
15


LNU106
14483.2
3.927
0.059
9
LNU106
14484.3
5.251
0.041
13
LNU106
14484.3
42.0
0.031
15


LNU114
25042.1
3.824
0.009
6
LNU114
25041.2
5.048
0.584
9
LNU114
25041.2
40.4
0.544
10


LNU114
25041.2
3.809
0.559
6
LNU114
25042.1
5.030
0.127
9
LNU114
25042.1
37.8
0.797
3


LNU114
25041.1
3.764
0.650
4
LNU155
14525.1
5.470
0.145
18
LNU155
14525.1
43.8
0.125
20


LNU155
14525.1
3.907
0.281
8
LNU155
14523.5
5.131
0.517
11
LNU155
14523.5
41.1
0.480
12


LNU155
14523.5
3.768
0.668
4
LNU213
24654.4
5.291
0.068
14
LNU213
24654.4
42.3
0.053
16


LNU213
24652.4
3.789
0.690
5
LNU218
24781.7
5.948
0.000
28
LNU218
24781.7
47.6
0.000
30


LNU213
24654.4
3.787
0.032
5
LNU218
24781.4
5.916
0.220
28
LNU218
24781.4
47.3
0.206
29


LNU213
24653.2
3.731
0.735
3
LNU218
24784.2
5.573
0.127
20
LNU218
24784.2
44.6
0.109
22


LNU218
24781.7
4.252
0.031
18
LNU23
25163.5
5.631
0.533
22
LNU218
24781.2
37.5
0.666
3


LNU218
24781.4
4.196
0.104
16
LNU23
25163.6
5.104
0.623
10
LNU23
25163.5
45.1
0.515
23


LNU218
24784.2
3.967
0.003
10
LNU28
25171.2
5.541
0.000
20
LNU23
25163.6
40.8
0.589
12


LNU218
24781.2
3.729
0.137
3
LNU28
25171.1
5.522
0.000
19
LNU28
25171.2
44.3
0.000
21


LNU23
25163.5
4.157
0.442
15
LNU4
25133.3
5.633
0.001
22
LNU28
25171.1
44.2
0.001
21


LNU23
25163.6
3.923
0.471
9
LNU4
25134.1
4.919
0.614
6
LNU4
25133.3
45.1
0.001
23


LNU23
25162.1
3.663
0.407
2
LNU40
24794.3
5.925
0.016
28
LNU4
25134.1
39.4
0.557
8


LNU28
25171.1
4.244
0.001
18
LNU40
24794.4
5.510
0.201
19
LNU40
24794.3
47.4
0.012
30


LNU28
25171.2
4.046
0.000
12
LNU46
14462.5
7.136
0.169
54
LNU40
24794.4
41.5
0.529
13


LNU28
25174.5
3.751
0.795
4
LNU46
14464.4
6.666
0.333
44
LNU46
14462.5
57.1
0.163
56


LNU4
25133.3
4.119
0.000
14
LNU46
14463.1
5.094
0.053
10
LNU46
14464.4
53.3
0.325
46


LNU4
25134.1
3.793
0.427
5
LNU46
14464.1
5.091
0.285
10
LNU46
14463.1
40.8
0.042
11


LNU4
25131.1
3.711
0.305
3
LNU46
14462.1
4.978
0.516
8
LNU46
14464.1
40.7
0.244
11


LNU40
24794.3
4.091
0.000
13
LNU48
24801.4
6.013
0.000
30
LNU46
14462.1
39.8
0.463
9


LNU40
24794.4
4.033
0.018
12
LNU48
24802.1
5.221
0.008
13
LNU48
24801.4
48.1
0.000
31


LNU46
14462.5
4.468
0.163
24
LNU48
24804.4
4.891
0.171
6
LNU48
24802.1
41.8
0.007
14


LNU46
14464.4
4.460
0.263
24
LNU63
24814.2
5.537
0.003
20
LNU48
24804.4
39.1
0.131
7


LNU46
14464.1
3.912
0.179
8
LNU63
24814.3
5.022
0.132
8
LNU63
24814.2
44.3
0.002
21


LNU46
14463.1
3.806
0.215
6
LNU63
24811.2
4.848
0.409
5
LNU63
24814.3
40.2
0.102
10


LNU46
14462.1
3.803
0.317
5
LNU7
25081.1
5.350
0.006
16
LNU63
24811.2
38.8
0.326
6


LNU48
24801.4
4.262
0.000
18
LNU7
25082.2
5.052
0.320
9
LNU7
25081.1
42.8
0.005
17


LNU48
24802.1
3.989
0.000
11
LNU8
25062.1
5.062
0.031
9
LNU7
25082.2
40.4
0.275
10


LNU48
24804.4
3.707
0.232
3
LNU8
25063.6
5.017
0.229
8
LNU8
25062.1
40.5
0.027
11


LNU63
24814.2
4.060
0.000
13
LNU8
25062.2
4.960
0.219
7
LNU8
25063.6
40.1
0.185
10


LNU63
24814.3
3.864
0.004
7
LNU8
25061.2
4.837
0.300
4
LNU8
25062.2
39.7
0.172
8


LNU63
24811.2
3.717
0.286
3
LNU94
24833.3
5.355
0.037
16
LNU8
25061.2
38.7
0.226
6


LNU7
25081.1
3.996
0.002
11
LNU96
25071.2
5.810
0.305
25
LNU94
24833.3
40.1
0.178
10


LNU7
25082.2
3.864
0.348
7
LNU96
25073.4
4.985
0.729
8
LNU96
25071.2
46.5
0.290
27


LNU8
25063.6
3.835
0.028
6
LNU96
25071.3
4.854
0.524
5
LNU96
25073.4
39.9
0.693
9


LNU8
25061.2
3.731
0.387
3
CONT.
—
5.751
—
0
CONT.
—
46.0
—
0


LNU8
25062.2
3.731
0.092
3
LNU113
25631.7
6.330
0.502
10
LNU113
25631.7
50.6
0.502
10


LNU8
25062.1
3.659
0.573
1
LNU113
25631.3
6.104
0.634
6
LNU113
25631.3
48.8
0.634
6


LNU94
24833.3
3.913
0.030
9
LNU113
25631.1
5.964
0.236
4
LNU113
25631.1
47.7
0.236
4


LNU96
25071.2
4.171
0.281
16
LNU120
25463.6
5.862
0.757
2
LNU120
25463.6
46.9
0.757
2


LNU96
25073.4
3.883
0.603
8
LNU140
14115.1
6.053
0.639
5
LNU140
14115.1
48.4
0.639
5


LNU96
25071.3
3.827
0.491
6
LNU148
25685.6
7.625
0.100
33
LNU148
25685.6
61.0
0.100
33


CONT.
—
3.931
—
0
LNU148
25685.1
6.612
0.066
15
LNU148
25685.1
52.9
0.066
15


LNU113
25631.3
4.273
0.384
9
LNU148
25685.9
6.006
0.756
4
LNU148
25685.9
48.1
0.756
4


LNU113
25631.7
4.196
0.311
7
LNU287
24674.6
5.818
0.717
1
LNU287
24674.6
46.5
0.717
1


LNU113
25631.1
4.081
0.064
4
LNU5
14043.7
6.452
0.317
12
LNU5
14043.7
51.6
0.317
12


LNU120
25463.3
4.027
0.218
2
LNU68
14035.5
5.933
0.381
3
LNU68
14035.5
47.5
0.381
3


LNU124
14501.1
4.008
0.346
2
LNU74
25444.1
6.292
0.127
9
LNU74
25444.1
50.3
0.127
9


LNU132
14102.6
4.040
0.554
3
LNU74
25443.2
5.820
0.674
1
LNU74
25443.2
46.6
0.674
1


LNU140
14115.1
4.026
0.704
2
LNU98
25763.2
5.892
0.745
2
LNU98
25763.2
47.1
0.745
2


LNU148
25685.6
4.481
0.166
14
CONT.
—
5.257
—
0
CONT.
—
41.6
—
0


LNU148
25685.1
4.295
0.056
9
LNU113
25631.9
5.826
0.342
11
LNU113
25631.9
46.6
0.302
12


LNU287
24674.6
4.081
0.322
4
LNU148
25685.1
5.876
0.263
12
LNU148
25685.1
47.0
0.228
13


LNU5
14043.7
4.199
0.258
7
LNU72
24963.7
5.957
0.447
13
LNU72
24963.7
47.7
0.417
15


LNU68
14035.5
4.069
0.056
4
LNU72
24962.3
5.592
0.691
6
LNU72
24962.3
44.7
0.643
8


LNU74
25444.1
4.110
0.149
5
LNU98
25763.2
5.771
0.476
10
LNU98
25763.2
46.2
0.434
11


LNU74
25443.2
3.977
0.479
1
CONT.
—
4.937
—
0
CONT.
—
38.1
—
0


LNU98
25763.2
4.019
0.561
2
LNU117
25931.4
8.201
0.038
66
LNU117
25931.4
65.6
0.031
72


CONT.
—
3.861
—
0
LNU117
25931.2
7.079
0.047
43
LNU117
25931.2
56.6
0.055
49


LNU113
25631.9
4.102
0.390
6
LNU117
25933.3
6.946
0.038
41
LNU117
25933.3
55.6
0.058
46


LNU113
25631.3
3.943
0.755
2
LNU117
25931.1
6.455
0.297
31
LNU117
25931.1
51.6
0.257
35


LNU148
25685.1
4.083
0.380
6
LNU117
25932.4
6.152
0.430
25
LNU117
25932.4
49.2
0.381
29


LNU72
24963.7
4.114
0.536
7
LNU122
25333.2
7.762
0.015
57
LNU122
25333.2
62.1
0.029
63


LNU98
25763.2
4.030
0.446
4
LNU122
25332.1
7.548
0.257
53
LNU122
25332.1
60.4
0.226
58


CONT.
—
3.681
—
0
LNU122
25332.2
6.704
0.053
36
LNU122
25332.2
53.6
0.083
41


LNU117
25931.4
4.672
0.079
27
LNU122
25332.5
6.165
0.112
25
LNU122
25332.5
49.3
0.145
29


LNU117
25933.3
4.364
0.041
19
LNU122
25333.1
6.130
0.142
24
LNU122
25333.1
49.0
0.160
29


LNU117
25931.2
4.320
0.047
17
LNU125
25941.4
8.098
0.097
64
LNU125
25941.4
64.8
0.074
70


LNU117
25932.4
4.197
0.333
14
LNU125
25943.3
7.176
0.030
45
LNU125
25943.3
57.4
0.053
51


LNU117
25931.1
4.189
0.192
14
LNU125
25943.2
6.828
0.370
38
LNU125
25943.2
54.6
0.334
43


LNU122
25332.1
4.536
0.164
23
LNU125
25941.2
6.448
0.309
31
LNU125
25941.2
51.6
0.268
35


LNU122
25333.2
4.522
0.024
23
LNU125
25944.3
6.167
0.380
25
LNU125
25944.3
49.3
0.333
29


LNU122
25332.2
4.252
0.076
16
LNU138
14074.5
7.777
0.014
58
LNU138
14074.5
62.2
0.029
63


LNU122
25333.1
4.135
0.102
12
LNU138
14074.6
7.468
0.181
51
LNU138
14074.6
59.7
0.149
57


LNU122
25332.5
4.098
0.228
11
LNU138
14071.5
6.727
0.051
36
LNU138
14071.5
53.8
0.074
41


LNU125
25941.4
4.714
0.088
28
LNU138
14072.8
5.184
0.664
5
LNU138
14072.8
41.5
0.580
9


LNU125
25943.3
4.442
0.039
21
LNU180
24721.2
9.058
0.043
83
LNU138
14072.5
40.1
0.740
5


LNU125
25943.2
4.326
0.317
18
LNU180
24723.1
8.587
0.010
74
LNU180
24721.2
72.5
0.030
90


LNU125
25941.2
4.212
0.315
14
LNU180
24721.4
8.409
0.017
70
LNU180
24723.1
68.7
0.014
80


LNU125
25944.3
4.191
0.197
14
LNU180
24722.2
7.901
0.179
60
LNU180
24721.4
67.3
0.018
76


LNU138
14074.5
4.654
0.016
26
LNU180
24724.1
7.457
0.117
51
LNU180
24722.2
63.2
0.150
66


LNU138
14074.6
4.553
0.132
24
LNU220
25405.2
7.726
0.048
56
LNU180
24724.1
59.7
0.093
56


LNU138
14071.5
4.284
0.060
16
LNU220
25405.5
7.662
0.086
55
LNU220
25405.2
61.8
0.041
62


LNU138
14072.5
3.780
0.639
3
LNU220
25405.1
7.524
0.216
52
LNU220
25405.5
61.3
0.068
61


LNU138
14072.8
3.760
0.685
2
LNU220
25405.6
6.268
0.091
27
LNU220
25405.1
60.2
0.184
58


LNU180
24721.2
5.125
0.010
39
LNU220
25405.3
5.915
0.406
20
LNU220
25405.6
50.1
0.130
32


LNU180
24723.1
4.878
0.018
33
LNU230
25413.1
8.332
0.014
69
LNU220
25405.3
47.3
0.356
24


LNU180
24721.4
4.870
0.010
32
LNU230
25412.1
7.791
0.079
58
LNU230
25413.1
66.7
0.018
75


LNU180
24722.2
4.783
0.121
30
LNU230
25415.1
7.230
0.167
46
LNU230
25412.1
62.3
0.061
63


LNU180
24724.1
4.538
0.062
23
LNU230
25412.2
6.904
0.045
40
LNU230
25415.1
57.8
0.137
52


LNU220
25405.5
4.611
0.090
25
LNU230
25413.2
6.826
0.274
38
LNU230
25413.2
54.6
0.236
43


LNU220
25405.1
4.497
0.217
22
LNU234
25014.8
7.333
0.034
49
LNU230
25412.2
51.7
0.100
36


LNU220
25405.2
4.485
0.035
22
LNU234
25014.1
6.560
0.125
33
LNU234
25014.8
58.7
0.053
54


LNU220
25405.6
4.220
0.089
15
LNU234
25014.6
6.438
0.079
30
LNU234
25014.1
52.5
0.119
38


LNU220
25405.3
4.043
0.244
10
LNU234
25014.4
6.183
0.121
25
LNU234
25014.6
51.5
0.113
35


LNU230
25413.1
4.814
0.021
31
LNU234
25014.5
5.958
0.451
21
LNU234
25014.4
49.5
0.146
30


LNU230
25412.1
4.738
0.044
29
LNU25
14082.8
7.851
0.067
59
LNU234
25014.5
47.7
0.398
25


LNU230
25415.1
4.453
0.193
21
LNU25
14083.7
7.683
0.025
56
LNU25
14082.8
62.8
0.052
65


LNU230
25412.2
4.390
0.049
19
LNU25
14082.9
7.494
0.021
52
LNU25
14083.7
61.5
0.041
61


LNU230
25413.2
4.226
0.340
15
LNU25
14084.6
7.440
0.229
51
LNU25
14082.9
59.9
0.040
57


LNU234
25014.8
4.643
0.017
26
LNU25
14083.1
7.369
0.024
49
LNU25
14084.6
59.5
0.196
56


LNU234
25014.1
4.390
0.202
19
LNU254
25782.4
8.004
0.020
62
LNU25
14083.1
58.9
0.044
55


LNU234
25014.6
4.206
0.086
14
LNU254
25781.3
7.690
0.025
56
LNU254
25782.4
64.0
0.034
68


LNU234
25014.5
4.196
0.250
14
LNU254
25781.5
7.136
0.064
45
LNU254
25781.3
61.5
0.031
61


LNU234
25014.4
4.160
0.121
13
LNU254
25782.5
6.589
0.062
33
LNU254
25781.5
57.1
0.062
50


LNU25
14082.8
4.776
0.101
30
LNU254
25783.1
6.556
0.250
33
LNU254
25782.5
52.7
0.086
38


LNU25
14082.9
4.731
0.018
29
LNU263
25794.8
7.806
0.182
58
LNU254
25783.1
52.5
0.214
38


LNU25
14083.1
4.630
0.018
26
LNU263
25794.3
7.786
0.015
58
LNU263
25794.8
62.4
0.152
64


LNU25
14083.7
4.596
0.033
25
LNU263
25791.3
7.761
0.041
57
LNU263
25794.3
62.3
0.027
63


LNU25
14084.6
4.556
0.263
24
LNU263
25794.6
7.305
0.024
48
LNU263
25791.3
62.1
0.037
63


LNU254
25782.4
4.741
0.020
29
LNU263
25792.2
7.079
0.031
43
LNU263
25794.6
58.4
0.045
53


LNU254
25781.3
4.696
0.046
28
LNU267
25804.4
7.228
0.038
46
LNU263
25792.2
56.6
0.051
49


LNU254
25781.5
4.485
0.027
22
LNU267
25804.3
7.196
0.098
46
LNU267
25804.4
57.8
0.058
52


LNU254
25782.5
4.354
0.060
18
LNU267
25801.1
6.656
0.227
35
LNU267
25804.3
57.6
0.082
51


LNU254
25783.1
4.174
0.325
13
LNU267
25803.1
6.585
0.059
33
LNU267
25801.1
53.2
0.193
40


LNU263
25794.8
4.696
0.165
28
LNU267
25802.1
6.188
0.147
25
LNU267
25803.1
52.7
0.092
38


LNU263
25794.3
4.651
0.022
26
LNU271
25911.4
8.631
0.011
75
LNU267
25802.1
49.5
0.156
30


LNU263
25791.3
4.640
0.023
26
LNU271
25912.1
7.598
0.052
54
LNU271
25911.4
69.1
0.021
81


LNU263
25794.6
4.565
0.022
24
LNU271
25913.3
6.587
0.069
33
LNU271
25912.1
57.3
0.192
50


LNU263
25792.2
4.422
0.034
20
LNU271
25912.2
6.006
0.141
22
LNU271
25913.3
52.7
0.089
38


LNU267
25804.3
4.532
0.064
23
LNU271
25913.2
5.306
0.743
7
LNU271
25912.2
48.1
0.181
26


LNU267
25804.4
4.525
0.032
23
LNU278
25814.3
8.097
0.011
64
LNU271
25913.2
42.4
0.653
11


LNU267
25803.1
4.381
0.052
19
LNU278
25812.3
8.034
0.021
63
LNU278
25814.3
64.8
0.022
70


LNU267
25801.1
4.216
0.143
15
LNU278
25812.2
6.641
0.088
35
LNU278
25812.3
64.3
0.034
69


LNU267
25802.1
4.181
0.156
14
LNU278
25814.1
6.351
0.218
29
LNU278
25812.2
53.1
0.094
39


LNU271
25911.4
5.046
0.006
37
LNU278
25813.2
5.577
0.364
13
LNU278
25814.1
50.8
0.191
33


LNU271
25912.1
4.508
0.033
22
LNU36
25562.9
7.926
0.019
61
LNU278
25813.2
44.6
0.349
17


LNU271
25913.3
4.382
0.064
19
LNU36
25562.3
6.823
0.049
38
LNU36
25562.9
63.4
0.033
66


LNU271
25912.2
4.152
0.102
13
LNU36
25562.4
6.281
0.088
27
LNU36
25562.3
54.6
0.076
43


LNU271
25913.2
3.894
0.579
6
LNU36
25562.7
5.943
0.154
20
LNU36
25562.4
50.3
0.125
32


LNU278
25812.3
4.752
0.020
29
LNU36
25561.2
5.749
0.222
16
LNU36
25562.7
47.5
0.194
25


LNU278
25814.3
4.655
0.028
26
LNU43
14422.8
7.369
0.026
49
LNU36
25561.2
46.0
0.256
21


LNU278
25812.2
4.267
0.059
16
LNU43
14422.9
6.842
0.040
39
LNU43
14422.8
59.0
0.046
55


LNU278
25814.1
4.121
0.113
12
LNU43
14421.1
6.814
0.042
38
LNU43
14422.9
54.7
0.065
44


LNU278
25813.2
3.927
0.294
7
LNU43
14423.6
6.361
0.080
29
LNU43
14421.1
54.5
0.068
43


LNU36
25562.9
4.633
0.027
26
LNU43
14423.7
6.061
0.312
23
LNU43
14423.6
50.9
0.113
33


LNU36
25562.3
4.246
0.070
15
LNU45
25052.12
7.449
0.059
51
LNU43
14423.7
48.5
0.275
27


LNU36
25561.2
4.090
0.137
11
LNU45
25052.8
7.250
0.025
47
LNU45
25052.12
59.6
0.052
56


LNU36
25562.7
4.023
0.177
9
LNU45
25052.9
6.722
0.055
36
LNU45
25052.8
58.0
0.045
52


LNU36
25562.4
4.008
0.221
9
LNU45
25053.4
6.464
0.464
31
LNU45
25052.9
53.8
0.075
41


LNU43
14422.8
4.553
0.028
24
LNU45
25052.11
5.637
0.269
14
LNU45
25053.4
51.7
0.424
36


LNU43
14421.1
4.514
0.032
23
LNU67
25821.5
6.477
0.348
31
LNU45
25052.11
45.1
0.297
18


LNU43
14422.9
4.354
0.042
18
LNU67
25824.5
6.258
0.293
27
LNU67
25821.5
51.8
0.306
36


LNU43
14423.6
4.283
0.088
16
LNU67
25823.5
6.011
0.155
22
LNU67
25824.5
50.1
0.255
31


LNU43
14423.7
4.074
0.318
11
LNU67
25821.4
5.761
0.238
17
LNU67
25823.5
48.1
0.181
26


LNU45
25052.12
4.543
0.106
23
LNU67
25824.3
5.603
0.597
13
LNU67
25821.4
46.1
0.257
21


LNU45
25052.8
4.344
0.057
18
CONT.
—
3.923
—
0
LNU67
25824.3
44.8
0.528
18


LNU45
25052.9
4.226
0.108
15
LNU100
14474.2
5.788
0.078
48
CONT.
—
31.4
—
0


LNU45
25053.4
4.169
0.483
13
LNU100
14472.1
5.604
0.332
43
LNU100
14474.2
46.3
0.078
48


LNU45
25052.11
3.898
0.323
6
LNU100
14473.3
5.227
0.373
33
LNU100
14472.1
44.8
0.332
43


LNU67
25821.5
4.189
0.385
14
LNU100
14473.1
5.199
0.202
33
LNU100
14473.3
41.8
0.373
33


LNU67
25824.5
4.160
0.342
13
LNU100
14471.4
4.583
0.510
17
LNU100
14473.1
41.6
0.202
33


LNU67
25823.5
4.049
0.152
10
LNU104
25032.2
4.811
0.483
23
LNU100
14471.4
36.7
0.510
17


LNU67
25824.3
4.010
0.423
9
LNU104
25033.1
4.727
0.319
21
LNU104
25032.2
38.5
0.483
23


LNU67
25821.4
3.971
0.295
8
LNU104
25032.1
4.607
0.401
17
LNU104
25033.1
37.8
0.319
21


CONT.
—
3.345
—
0
LNU104
25033.3
4.515
0.021
15
LNU104
25032.1
36.9
0.401
17


LNU100
14474.2
4.215
0.024
26
LNU106
14483.2
5.906
0.009
51
LNU104
25033.3
36.1
0.021
15


LNU100
14472.1
4.080
0.377
22
LNU106
14481.1
5.002
0.460
28
LNU106
14483.2
47.3
0.009
51


LNU100
14473.3
3.988
0.272
19
LNU106
14483.5
4.726
0.003
20
LNU106
14481.1
40.0
0.460
28


LNU100
14473.1
3.860
0.145
15
LNU106
14484.3
4.471
0.599
14
LNU106
14483.5
37.8
0.003
20


LNU100
14471.4
3.657
0.440
9
LNU114
25044.11
5.196
0.404
32
LNU106
14484.3
35.8
0.599
14


LNU104
25033.1
3.784
0.440
13
LNU114
25041.2
5.087
0.105
30
LNU114
25044.11
41.6
0.404
32


LNU104
25033.3
3.769
0.127
13
LNU114
25042.1
4.666
0.316
19
LNU114
25041.2
40.7
0.105
30


LNU104
25032.1
3.733
0.185
12
LNU114
25041.1
4.424
0.790
13
LNU114
25042.1
37.3
0.316
19


LNU104
25032.2
3.695
0.465
10
LNU114
25044.4
4.267
0.800
9
LNU114
25041.1
35.4
0.790
13


LNU106
14483.2
4.299
0.001
29
LNU114
25932.4
5.264
0.026
34
LNU114
25044.4
34.1
0.800
9


LNU106
14481.1
3.952
0.369
18
LNU117
25931.4
5.003
0.007
28
LNU117
25932.4
42.1
0.026
34


LNU106
14483.5
3.741
0.029
12
LNU117
25931.1
4.406
0.731
12
LNU117
25931.4
40.0
0.007
28


LNU106
14484.3
3.573
0.544
7
LNU117
25931.2
4.256
0.596
9
LNU117
25931.1
35.2
0.731
12


LNU114
25044.11
3.992
0.266
19
LNU155
14523.5
5.064
0.371
29
LNU117
25931.2
34.1
0.596
9


LNU114
25041.2
3.908
0.005
17
LNU155
14524.8
4.210
0.411
7
LNU155
14523.5
40.5
0.371
29


LNU114
25042.1
3.783
0.193
13
LNU180
24723.1
4.999
0.001
27
LNU155
14524.8
33.7
0.411
7


LNU114
25041.1
3.667
0.691
10
LNU180
24722.2
4.503
0.696
15
LNU180
24723.1
40.0
0.001
27


LNU117
25932.4
4.072
0.091
22
LNU218
24781.4
5.394
0.065
38
LNU180
24722.2
36.0
0.696
15


LNU117
25931.4
3.797
0.001
14
LNU218
24781.1
4.950
0.283
26
LNU218
24781.4
43.2
0.065
38


LNU117
25931.1
3.637
0.607
9
LNU218
24781.6
4.116
0.510
5
LNU218
24781.1
39.6
0.283
26


LNU117
25931.2
3.493
0.531
4
LNU218
24781.2
4.052
0.541
3
LNU218
24781.6
32.9
0.510
5


LNU155
14523.5
3.825
0.342
14
LNU254
25782.5
4.883
0.061
24
LNU218
24781.2
32.4
0.541
3


LNU155
14524.8
3.615
0.063
8
LNU4
25134.3
5.451
0.299
39
LNU254
25782.5
39.1
0.061
24


LNU180
24723.1
3.874
0.067
16
LNU4
25134.2
4.702
0.210
20
LNU4
25134.3
43.6
0.299
39


LNU180
24722.2
3.708
0.619
11
LNU4
25131.1
4.628
0.422
18
LNU4
25134.2
37.6
0.210
20


LNU218
24781.4
4.130
0.080
23
LNU4
25133.3
4.261
0.501
9
LNU4
25131.1
37.0
0.422
18


LNU218
24781.1
4.061
0.057
21
LNU40
24794.3
4.596
0.661
17
LNU4
25133.3
34.1
0.501
9


LNU218
24781.2
3.582
0.031
7
LNU40
24792.1
4.508
0.017
15
LNU40
24794.3
36.8
0.661
17


LNU218
24781.6
3.522
0.244
5
LNU40
24794.4
4.144
0.345
6
LNU40
24792.1
36.1
0.017
15


LNU254
25782.5
3.825
0.120
14
LNU46
14462.5
5.330
0.315
36
LNU40
24794.4
33.2
0.345
6


LNU254
25782.4
3.387
0.697
1
LNU46
14464.4
4.290
0.256
9
LNU46
14462.5
42.6
0.315
36


LNU4
25134.3
4.057
0.310
21
LNU46
14462.1
4.285
0.114
9
LNU46
14464.4
34.3
0.256
9


LNU4
25134.2
3.667
0.282
10
LNU48
24801.4
4.780
0.139
22
LNU46
14462.1
34.3
0.114
9


LNU4
25131.1
3.643
0.535
9
LNU48
24803.2
4.595
0.596
17
LNU48
24801.4
38.2
0.139
22


LNU4
25133.3
3.533
0.527
6
LNU63
24812.3
5.325
0.111
36
LNU48
24803.2
36.8
0.596
17


LNU40
24794.3
3.803
0.557
14
LNU63
24814.7
4.440
0.426
13
LNU63
24812.3
42.6
0.111
36


LNU40
24792.1
3.682
0.005
10
LNU7
25082.2
4.973
0.460
27
LNU63
24814.7
35.5
0.426
13


LNU40
24794.4
3.484
0.177
4
LNU7
25083.1
4.472
0.220
14
LNU7
25082.2
39.8
0.460
27


LNU46
14462.5
3.977
0.353
19
LNU7
25083.3
4.378
0.198
12
LNU7
25083.1
35.8
0.220
14


LNU46
14462.1
3.575
0.041
7
LNU8
25063.6
5.383
0.119
37
LNU7
25083.3
35.0
0.198
12


LNU46
14464.4
3.512
0.213
5
LNU8
25061.2
4.703
0.003
20
LNU8
25063.6
43.1
0.119
37


LNU48
24803.2
3.731
0.520
12
LNU94
24833.3
4.639
0.511
18
LNU8
25061.2
37.6
0.003
20


LNU48
24801.4
3.724
0.156
11
CONT.
—
5.138
—
0
LNU94
24833.3
37.1
0.511
18


LNU48
24802.2
3.457
0.760
3
LNU122
25332.1
6.084
0.057
18
CONT.
—
41.1
—
0


LNU63
24812.3
4.044
0.067
21
LNU122
25332.2
6.037
0.049
18
LNU122
25332.1
48.7
0.057
18


LNU63
24814.7
3.580
0.458
7
LNU122
25332.5
5.627
0.601
10
LNU122
25332.2
48.3
0.049
18


LNU63
24814.2
3.498
0.361
5
LNU125
25943.2
5.541
0.155
8
LNU122
25332.5
45.0
0.601
10


LNU7
25082.2
3.789
0.443
13
LNU125
25941.4
5.481
0.153
7
LNU125
25943.2
44.3
0.155
8


LNU7
25083.1
3.697
0.020
11
LNU138
14074.5
5.527
0.128
8
LNU125
25941.4
43.8
0.153
7


LNU7
25083.3
3.566
0.325
7
LNU138
14071.5
5.204
0.795
1
LNU138
14074.5
44.2
0.128
8


LNU8
25063.6
4.011
0.034
20
LNU157
24982.1
5.356
0.680
4
LNU138
14071.5
41.6
0.795
1


LNU8
25061.2
3.745
0.002
12
LNU178
14614.5
5.971
0.096
16
LNU157
24982.1
42.8
0.680
4


LNU94
24833.3
3.572
0.610
7
LNU178
14611.5
5.656
0.022
10
LNU178
14614.5
47.8
0.096
16


CONT.
—
3.921
—
0
LNU178
14612.1
5.477
0.551
7
LNU178
14611.5
45.3
0.022
10


LNU10
25123.5
4.081
0.621
4
LNU178
14611.4
5.445
0.126
6
LNU178
14612.1
43.8
0.551
7


LNU122
25332.1
4.326
0.175
10
LNU220
25405.6
5.656
0.282
10
LNU178
14611.4
43.6
0.126
6


LNU122
25332.2
4.305
0.000
10
LNU220
25405.1
5.643
0.024
10
LNU220
25405.6
45.2
0.282
10


LNU122
25332.5
4.214
0.324
7
LNU220
25405.5
5.597
0.084
9
LNU220
25405.1
45.1
0.024
10


LNU122
25333.2
3.972
0.796
1
LNU220
25405.2
5.420
0.567
5
LNU220
25405.5
44.8
0.084
9


LNU125
25941.4
4.108
0.072
5
LNU234
25014.6
5.343
0.764
4
LNU220
25405.2
43.4
0.567
5


LNU125
25943.2
4.065
0.372
4
LNU236
25424.2
6.609
0.163
29
LNU234
25014.6
42.7
0.764
4


LNU138
14074.5
4.227
0.004
8
LNU236
25422.4
5.751
0.104
12
LNU236
25424.2
52.9
0.163
29


LNU138
14071.5
4.055
0.110
3
LNU236
25425.4
5.715
0.461
11
LNU236
25422.4
46.0
0.104
12


LNU138
14074.6
3.995
0.626
2
LNU236
25423.3
5.617
0.562
9
LNU236
25425.4
45.7
0.461
11


LNU157
24982.1
4.149
0.283
6
LNU24
24974.2
5.406
0.476
5
LNU236
25423.3
44.9
0.562
9


LNU178
14614.5
4.306
0.077
10
LNU25
14082.8
6.389
0.000
24
LNU24
24974.2
43.2
0.476
5


LNU178
14611.5
4.250
0.119
8
LNU25
14082.9
5.548
0.099
8
LNU25
14082.8
51.1
0.000
24


LNU178
14611.4
4.135
0.015
5
LNU25
14084.6
5.424
0.348
6
LNU25
14082.9
44.4
0.099
8


LNU178
14612.1
4.004
0.628
2
LNU271
25911.4
5.716
0.647
11
LNU25
14084.6
43.4
0.348
6


LNU220
25405.6
4.098
0.215
5
LNU278
25814.1
5.701
0.013
11
LNU271
25911.4
45.7
0.647
11


LNU220
25405.5
4.062
0.115
4
LNU278
25814.3
5.444
0.283
6
LNU278
25814.1
45.6
0.013
11


LNU220
25405.1
4.012
0.543
2
LNU278
25812.2
5.272
0.492
3
LNU278
25814.3
43.5
0.283
6


LNU220
25405.2
3.998
0.620
2
LNU43
14423.7
5.559
0.132
8
LNU278
25812.2
42.2
0.492
3


LNU234
25014.6
4.021
0.585
3
LNU45
25052.12
7.025
0.000
37
LNU43
14423.7
44.5
0.132
8


LNU234
25014.5
3.951
0.735
1
LNU45
25053.4
6.516
0.308
27
LNU45
25052.12
56.2
0.000
37


LNU236
25424.2
4.580
0.101
17
LNU45
25052.11
5.797
0.179
13
LNU45
25053.4
52.1
0.308
27


LNU236
25423.3
4.191
0.440
7
LNU45
25052.9
5.597
0.032
9
LNU45
25052.11
46.4
0.179
13


LNU236
25425.4
4.116
0.531
5
LNU67
25824.3
6.189
0.000
20
LNU45
25052.9
44.8
0.032
9


LNU236
25422.4
4.101
0.033
5
LNU67
25824.5
5.719
0.011
11
LNU67
25824.3
49.5
0.000
20


LNU24
24974.2
4.058
0.091
3
LNU67
25821.4
5.563
0.307
8
LNU67
25824.5
45.7
0.011
11


LNU24
24972.1
4.010
0.494
2
LNU9
25001.1
5.942
0.008
16
LNU67
25821.4
44.5
0.307
8


LNU25
14082.8
4.402
0.000
12
LNU9
25001.7
5.900
0.329
15
LNU9
25001.1
47.5
0.008
16


LNU25
14082.9
4.236
0.001
8
LNU9
25003.1
5.374
0.723
5
LNU9
25001.7
47.2
0.329
15


LNU25
14084.6
4.023
0.394
3
CONT.
—
5.507
—
0
LNU9
25003.1
43.0
0.723
5


LNU25
14083.7
3.969
0.736
1
LNU10
25123.6
6.580
0.085
19
CONT.
—
44.1
—
0


LNU267
25804.4
3.994
0.576
2
LNU10
25123.5
5.880
0.455
7
LNU10
25123.6
52.6
0.085
19


LNU271
25911.4
4.261
0.337
9
LNU157
24982.8
7.166
0.005
30
LNU10
25123.5
47.0
0.455
7


LNU271
25912.1
4.121
0.475
5
LNU157
24982.4
6.039
0.298
10
LNU157
24982.8
57.3
0.005
30


LNU278
25813.2
4.096
0.393
4
LNU157
24983.3
5.728
0.725
4
LNU157
24982.4
48.3
0.298
10


LNU278
25814.3
4.086
0.051
4
LNU173
25451.1
6.406
0.534
16
LNU157
24983.3
45.8
0.725
4


LNU278
25814.1
4.075
0.297
4
LNU178
14611.4
7.845
0.315
42
LNU173
25451.1
51.2
0.534
16


LNU278
25812.2
3.990
0.365
2
LNU178
14611.5
7.119
0.096
29
LNU178
14611.4
62.8
0.315
42


LNU43
14423.7
4.174
0.132
6
LNU178
14611.1
7.075
0.374
28
LNU178
14611.5
57.0
0.096
29


LNU43
14422.9
4.066
0.685
4
LNU184
25393.2
6.860
0.015
25
LNU178
14611.1
56.6
0.374
28


LNU45
25052.12
4.606
0.005
17
LNU184
25393.3
6.648
0.354
21
LNU184
25393.2
54.9
0.015
25


LNU45
25053.4
4.431
0.260
13
LNU184
25393.1
6.580
0.617
19
LNU184
25393.3
53.2
0.354
21


LNU45
25052.11
4.149
0.297
6
LNU184
25395.1
6.315
0.563
15
LNU184
25393.1
52.6
0.617
19


LNU45
25052.9
4.127
0.022
5
LNU230
25413.1
7.185
0.026
30
LNU184
25395.1
50.5
0.563
15


LNU67
25824.3
4.354
0.064
11
LNU230
25413.2
7.178
0.136
30
LNU230
25413.1
57.5
0.026
30


LNU67
25824.5
4.312
0.001
10
LNU230
25412.1
6.604
0.043
20
LNU230
25413.2
57.4
0.136
30


LNU67
25821.4
4.183
0.273
7
LNU230
25412.2
6.600
0.298
20
LNU230
25412.1
52.8
0.043
20


LNU9
25001.7
4.232
0.226
8
LNU230
25415.1
6.466
0.149
17
LNU230
25412.2
52.8
0.298
20


LNU9
25001.1
4.204
0.007
7
LNU236
25425.4
8.219
0.000
49
LNU230
25415.1
51.7
0.149
17


LNU9
25003.1
4.068
0.556
4
LNU236
25423.3
6.874
0.278
25
LNU236
25425.4
65.8
0.000
49


LNU9
25001.2
4.025
0.660
3
LNU236
25422.4
6.448
0.170
17
LNU236
25423.3
55.0
0.278
25


CONT.
—
3.944
—
0
LNU236
25424.2
6.380
0.075
16
LNU236
25422.4
51.6
0.170
17


LNU10
25123.6
4.138
0.332
5
LNU24
24974.2
7.569
0.002
37
LNU236
25424.2
51.0
0.075
16


LNU157
24982.8
4.444
0.034
13
LNU24
24971.3
6.403
0.230
16
LNU24
24974.2
60.6
0.002
37


LNU157
24982.4
4.193
0.371
6
LNU24
24971.2
5.754
0.601
4
LNU24
24971.3
51.2
0.230
16


LNU173
25451.1
4.512
0.313
14
LNU24
24972.1
5.719
0.665
4
LNU24
24971.2
46.0
0.601
4


LNU173
25451.11
4.050
0.602
3
LNU263
25791.3
7.019
0.194
27
LNU24
24972.1
45.8
0.665
4


LNU178
14611.4
4.797
0.243
22
LNU263
25794.3
6.800
0.159
23
LNU263
25791.3
56.2
0.194
27


LNU178
14611.5
4.523
0.095
15
LNU263
25794.8
6.413
0.261
16
LNU263
25794.3
54.4
0.159
23


LNU178
14611.1
4.401
0.349
12
LNU263
25792.2
6.090
0.249
11
LNU263
25794.8
51.3
0.261
16


LNU184
25393.2
4.407
0.052
12
LNU276
25433.1
6.838
0.133
24
LNU263
25792.2
48.7
0.249
11


LNU184
25393.3
4.363
0.445
11
LNU276
25431.1
6.701
0.131
22
LNU276
25433.1
54.7
0.133
24


LNU184
25395.1
4.336
0.494
10
LNU276
25433.3
6.144
0.175
12
LNU276
25431.1
53.6
0.131
22


LNU184
25393.1
4.294
0.623
9
LNU279
25484.3
7.208
0.010
31
LNU276
25433.3
49.1
0.175
12


LNU20
24933.4
4.011
0.756
2
LNU279
25481.3
7.101
0.255
29
LNU279
25484.3
57.7
0.010
31


LNU230
25413.2
4.579
0.204
16
LNU279
25481.5
6.775
0.173
23
LNU279
25481.3
56.8
0.255
29


LNU230
25413.1
4.544
0.071
15
LNU279
25481.4
6.607
0.242
20
LNU279
25481.5
54.2
0.173
23


LNU230
25412.1
4.308
0.113
9
LNU279
25481.2
6.433
0.660
17
LNU279
25481.4
52.9
0.242
20


LNU230
25412.2
4.279
0.300
8
LNU36
25562.3
7.147
0.344
30
LNU279
25481.2
51.5
0.660
17


LNU230
25415.1
4.195
0.242
6
LNU36
25562.7
7.019
0.528
27
LNU36
25562.3
57.2
0.344
30


LNU236
25425.4
4.953
0.001
26
LNU36
25561.2
6.466
0.436
17
LNU36
25562.7
56.2
0.528
27


LNU236
25423.3
4.397
0.278
11
LNU56
24694.1
6.943
0.530
26
LNU36
25561.2
51.7
0.436
17


LNU236
25422.4
4.229
0.174
7
LNU56
24693.1
6.813
0.402
24
LNU56
24694.1
55.5
0.530
26


LNU236
25424.2
4.222
0.203
7
LNU56
24691.2
6.571
0.207
19
LNU56
24693.1
54.5
0.402
24


LNU24
24974.2
4.724
0.003
20
LNU56
24694.2
6.087
0.583
11
LNU56
24691.2
52.6
0.207
19


LNU24
24971.3
4.446
0.030
13
LNU73
25755.1
8.249
0.000
50
LNU56
24694.2
48.7
0.583
11


LNU24
24971.4
4.323
0.630
10
LNU73
25751.1
7.035
0.007
28
LNU73
25755.1
66.0
0.000
50


LNU24
24971.2
4.177
0.306
6
LNU73
25751.9
7.002
0.057
27
LNU73
25751.1
56.3
0.007
28


LNU24
24972.1
4.036
0.670
2
LNU73
25751.8
6.744
0.478
22
LNU73
25751.9
56.0
0.057
27


LNU263
25791.3
4.414
0.206
12
LNU73
25754.2
6.571
0.510
19
LNU73
25751.8
53.9
0.478
22


LNU263
25794.3
4.382
0.058
11
LNU9
25001.1
7.060
0.030
28
LNU73
25754.2
52.6
0.510
19


LNU263
25794.8
4.364
0.160
11
LNU9
25001.7
7.045
0.272
28
LNU9
25001.1
56.5
0.030
28


LNU263
25794.6
4.133
0.664
5
LNU9
25001.2
6.492
0.050
18
LNU9
25001.7
56.4
0.272
28


LNU263
25792.2
4.105
0.417
4
CONT.
—
4.310
—
0
LNU9
25001.2
51.9
0.050
18


LNU276
25433.1
4.519
0.043
15
LNU131
14005.5
8.645
0.001
101
CONT.
—
34.1
—
0


LNU276
25431.1
4.420
0.058
12
LNU131
14005.2
6.311
0.274
46
LNU131
14005.5
69.2
0.001
103


LNU276
25433.3
4.065
0.546
3
LNU131
14002.15
5.784
0.140
34
LNU131
14005.2
50.5
0.269
48


LNU276
25433.2
4.058
0.721
3
LNU135
26204.2
8.965
0.258
108
LNU131
14002.15
46.3
0.137
36


LNU279
25481.3
4.560
0.121
16
LNU135
26203.6
6.304
0.010
46
LNU135
26204.2
71.7
0.257
110


LNU279
25484.3
4.429
0.045
12
LNU135
26203.4
6.171
0.448
43
LNU135
26203.6
50.4
0.010
48


LNU279
25481.4
4.402
0.080
12
LNU135
26203.1
5.922
0.000
37
LNU135
26203.4
49.4
0.441
45


LNU279
25481.5
4.265
0.415
8
LNU135
26203.3
5.009
0.698
16
LNU135
26203.1
47.4
0.000
39


LNU279
25481.2
4.214
0.715
7
LNU161
14553.5
6.325
0.483
47
LNU135
26203.3
40.1
0.682
17


LNU36
25562.3
4.718
0.145
20
LNU161
14553.6
5.177
0.010
20
LNU161
14553.5
50.6
0.476
48


LNU36
25562.7
4.472
0.522
13
LNU173
25451.2
4.840
0.090
12
LNU161
14553.6
41.4
0.006
21


LNU36
25561.2
4.392
0.185
11
LNU173
25451.5
4.762
0.536
10
LNU173
25451.2
38.7
0.062
13


LNU36
25562.9
4.067
0.606
3
LNU181
25771.2
6.501
0.126
51
LNU173
25451.5
38.1
0.503
12


LNU53
25674.1
4.109
0.434
4
LNU181
25771.11
5.656
0.001
31
LNU173
25451.11
35.2
0.767
3


LNU56
24694.1
4.545
0.384
15
LNU181
25771.6
5.580
0.028
29
LNU181
25771.2
52.0
0.125
52


LNU56
24693.1
4.456
0.334
13
LNU181
25774.1
5.207
0.009
21
LNU181
25771.11
45.3
0.001
33


LNU56
24691.2
4.309
0.335
9
LNU181
25771.8
4.592
0.664
7
LNU181
25771.6
44.6
0.026
31


LNU56
24694.2
4.131
0.682
5
LNU181
25771.5
4.521
0.499
5
LNU181
25774.1
41.7
0.005
22


LNU73
25755.1
4.907
0.002
24
LNU184
25395.1
7.171
0.277
66
LNU181
25771.8
36.7
0.620
8


LNU73
25751.1
4.566
0.021
16
LNU184
25394.3
7.043
0.350
63
LNU181
25771.5
36.2
0.405
6


LNU73
25751.9
4.543
0.012
15
LNU184
25394.1
6.482
0.289
50
LNU184
25395.1
57.4
0.274
68


LNU73
25751.8
4.499
0.356
14
LNU184
25393.3
6.186
0.067
44
LNU184
25394.3
56.3
0.346
65


LNU73
25754.2
4.393
0.435
11
LNU184
25393.2
5.114
0.046
19
LNU184
25394.1
51.9
0.285
52


LNU9
25001.1
4.617
0.008
17
LNU184
25393.1
5.009
0.591
16
LNU184
25393.3
49.5
0.067
45


LNU9
25001.7
4.463
0.087
13
LNU224
25871.3
7.087
0.000
64
LNU184
25393.2
40.9
0.036
20


LNU9
25001.2
4.305
0.092
9
LNU224
25874.1
6.965
0.008
62
LNU184
25393.1
40.1
0.572
17


LNU9
25001.3
4.015
0.712
2
LNU224
25872.2
6.385
0.001
48
LNU224
25871.3
56.7
0.000
66


CONT.
—
3.452
—
0
LNU224
25872.3
5.780
0.001
34
LNU224
25874.1
55.7
0.009
63


LNU131
14005.5
4.854
0.000
41
LNU224
25874.4
5.469
0.321
27
LNU224
25872.2
51.1
0.001
50


LNU131
14005.2
4.227
0.316
22
LNU246
25743.2
7.125
0.404
65
LNU224
25874.4
43.7
0.311
28


LNU131
14002.15
4.036
0.002
17
LNU246
25743.1
6.759
0.276
57
LNU224
25872.3
43.4
0.207
27


LNU135
26204.2
5.120
0.162
48
LNU246
25744.2
6.626
0.387
54
LNU246
25743.2
57.0
0.399
67


LNU135
26203.6
4.181
0.042
21
LNU246
25744.4
6.098
0.515
41
LNU246
25743.1
54.1
0.273
58


LNU135
26203.4
4.131
0.450
20
LNU246
25744.3
5.273
0.176
22
LNU246
25744.2
53.0
0.382
55


LNU135
26203.1
4.001
0.021
16
LNU250
25592.2
6.367
0.000
48
LNU246
25744.4
48.8
0.507
43


LNU135
26203.3
3.773
0.675
9
LNU250
25592.1
5.181
0.475
20
LNU246
25744.3
42.2
0.166
24


LNU161
14553.5
4.024
0.540
17
LNU250
25591.1
5.156
0.269
20
LNU250
25592.2
50.9
0.000
49


LNU161
14553.6
3.716
0.062
8
LNU260
26404.8
5.607
0.297
30
LNU250
25592.1
41.4
0.459
21


LNU173
25451.5
3.669
0.516
6
LNU260
26404.7
5.580
0.506
29
LNU250
25591.1
41.2
0.254
21


LNU173
25451.2
3.600
0.279
4
LNU260
26404.1
5.285
0.546
23
LNU260
26404.8
44.9
0.289
31


LNU173
25451.11
3.547
0.642
3
LNU260
26403.1
4.649
0.651
8
LNU260
26404.7
44.6
0.496
31


LNU181
25771.2
4.262
0.041
23
LNU260
26403.2
4.396
0.800
2
LNU260
26404.1
42.3
0.532
24


LNU181
25771.6
3.935
0.165
14
LNU276
25433.6
5.708
0.197
32
LNU260
26403.1
37.2
0.613
9


LNU181
25771.11
3.894
0.004
13
LNU276
25433.5
5.179
0.140
20
LNU260
26403.2
35.2
0.699
3


LNU181
25774.1
3.818
0.017
11
LNU276
25431.1
5.161
0.011
20
LNU276
25433.6
45.7
0.191
34


LNU181
25771.8
3.674
0.280
6
LNU276
25433.1
4.426
0.713
3
LNU276
25433.5
41.4
0.128
21


LNU184
25395.1
4.492
0.290
30
LNU279
25484.3
6.354
0.341
47
LNU276
25431.1
41.3
0.006
21


LNU184
25394.3
4.482
0.323
30
LNU279
25481.3
5.756
0.587
34
LNU276
25433.1
35.4
0.607
4


LNU184
25394.1
4.177
0.252
21
LNU279
25481.5
5.435
0.007
26
LNU279
25484.3
50.8
0.335
49


LNU184
25393.3
4.133
0.020
20
LNU279
25481.4
5.033
0.497
17
LNU279
25481.3
46.0
0.578
35


LNU184
25393.2
3.813
0.014
10
LNU3
26122.2
5.909
0.425
37
LNU279
25481.5
43.5
0.005
27


LNU184
25393.1
3.705
0.662
7
LNU33
25553.3
6.560
0.006
52
LNU279
25481.4
40.3
0.477
18


LNU224
25871.3
4.493
0.000
30
LNU33
25553.2
5.801
0.285
35
LNU3
26122.2
47.3
0.417
38


LNU224
25874.1
4.356
0.000
26
LNU33
25552.2
5.752
0.591
33
LNU33
25553.3
52.5
0.007
54


LNU224
25872.2
4.309
0.000
25
LNU33
25553.1
4.961
0.698
15
LNU33
25552.2
46.0
0.582
35


LNU224
25872.3
3.908
0.128
13
LNU53
25674.6
5.147
0.323
19
LNU33
25553.2
43.1
0.124
26


LNU224
25874.4
3.833
0.404
11
LNU56
24694.2
4.436
0.766
3
LNU33
25553.1
39.7
0.681
16


LNU246
25743.2
4.424
0.405
28
LNU73
25751.8
4.966
0.726
15
LNU53
25674.6
41.2
0.307
21


LNU246
25743.1
4.343
0.295
26
CONT.
—
7.009
—
0
LNU56
24694.2
35.5
0.687
4


LNU246
25744.2
4.321
0.372
25
LNU119
26144.2.
7.422
0.040
6
LNU56
24693.1
35.2
0.768
3


LNU246
25744.4
4.129
0.544
20
LNU130
24913.6.
8.010
0.031
14
LNU73
25751.8
39.7
0.710
16


LNU246
25744.3
3.791
0.027
10
LNU130
24912.7.
8.000
0.563
14
CONT.
—
56.1
—
0


LNU250
25592.2
4.190
0.000
21
LNU130
24914.5.
7.702
0.630
10
LNU119
26144.2.
59.4
0.040
6


LNU250
25591.1
3.850
0.300
12
LNU130
24911.7.
7.358
0.707
5
LNU130
24913.6.
64.1
0.031
14


LNU250
25592.1
3.821
0.500
11
LNU136
14511.10.
8.765
0.000
25
LNU130
24912.7.
64.0
0.563
14


LNU260
26404.7
3.957
0.497
15
LNU136
14515.5.
8.318
0.421
19
LNU130
24914.5.
61.6
0.630
10


LNU260
26404.8
3.890
0.165
13
LNU142
27541.1.
8.194
0.001
17
LNU130
24911.7.
58.9
0.707
5


LNU260
26404.1
3.770
0.526
9
LNU142
27546.1.
7.204
0.712
3
LNU136
14511.10.
70.1
0.000
25


LNU260
26403.1
3.649
0.489
6
LNU149
26175.3.
7.709
0.362
10
LNU136
14515.5.
66.5
0.421
19


LNU276
25433.6
3.990
0.171
16
LNU15
14123.11.
7.968
0.426
14
LNU142
27541.1.
65.6
0.001
17


LNU276
25431.1
3.781
0.144
10
LNU15
14123.13.
7.941
0.000
13
LNU142
27546.1.
57.6
0.712
3


LNU276
25433.5
3.747
0.110
9
LNU185
26474.2.
8.650
0.453
23
LNU149
26175.3.
57.6
0.306
3


LNU279
25484.3
4.239
0.327
23
LNU185
26475.1.
8.305
0.283
19
LNU15
14123.13.
63.5
0.000
13


LNU279
25481.3
4.086
0.519
18
LNU185
26474.1.
7.750
0.681
11
LNU15
14123.11.
60.1
0.749
7


LNU279
25481.5
3.904
0.006
13
LNU212
25833.2.
9.276
0.130
32
LNU185
26474.2.
69.2
0.453
23


LNU279
25481.4
3.752
0.483
9
LNU212
25834.4.
8.993
0.223
28
LNU185
26475.1.
66.4
0.283
19


LNU279
25481.2
3.509
0.721
2
LNU212
25834.5.
8.125
0.046
16
LNU185
26474.1.
62.0
0.681
11


LNU3
26122.2
4.147
0.367
20
LNU212
25832.1.
7.353
0.652
5
LNU212
25833.2.
74.2
0.130
32


LNU33
25553.3
4.335
0.017
26
LNU216
25985.4.
9.065
0.247
29
LNU212
25834.4.
71.9
0.223
28


LNU33
25552.2
4.067
0.541
18
LNU216
25982.1.
8.548
0.051
22
LNU212
25834.5.
65.0
0.046
16


LNU33
25553.2
3.973
0.017
15
LNU216
25984.1.
8.543
0.361
22
LNU212
25832.1.
58.8
0.652
5


LNU33
25553.1
3.716
0.678
8
LNU216
25984.6.
7.518
0.254
7
LNU216
25985.4.
72.5
0.247
29


LNU53
25674.6
3.826
0.360
11
LNU228
26224.7.
8.690
0.000
24
LNU216
25982.1.
68.4
0.051
22


LNU53
25674.3
3.496
0.751
1
LNU228
26222.4.
7.798
0.004
11
LNU216
25984.1.
68.3
0.361
22


LNU56
24694.2
3.551
0.538
3
LNU229
26112.3.
8.196
0.000
17
LNU216
25984.6.
60.1
0.254
7


LNU56
24693.1
3.515
0.785
2
LNU253
26241.1.
8.044
0.163
15
LNU228
26224.7.
69.5
0.000
24


LNU73
25751.8
3.799
0.677
10
LNU274
26261.3.
7.172
0.369
2
LNU228
26222.4.
62.4
0.004
11


CONT.
—
4.516
—
0
LNU280
26162.1.
8.341
0.473
19
LNU229
26112.3.
65.6
0.000
17


LNU119
26144.2.
4.661
0.249
3
LNU55
26015.1.
7.969
0.422
14
LNU253
26241.1.
64.4
0.163
15


LNU130
24912.7.
5.046
0.322
12
LNU81
26031.9.
7.582
0.756
8
LNU274
26261.3.
57.4
0.369
2


LNU130
24914.5.
4.894
0.471
8
LNU81
26034.2.
7.572
0.684
8
LNU280
26162.1.
66.7
0.473
19


LNU130
24911.7.
4.783
0.342
6
CONT.
—
6.912
—
0
LNU55
26015.1.
63.8
0.422
14


LNU130
24913.6.
4.731
0.324
5
LNU119
26141.1.
8.792
0.468
27
LNU81
26031.9.
60.7
0.756
8


LNU136
14511.10.
5.012
0.068
11
LNU119
26142.5.
7.376
0.586
7
LNU81
26034.2.
60.6
0.684
8


LNU136
14515.5.
4.893
0.486
8
LNU130
24913.5.
7.963
0.260
15
CONT.
—
55.3
—
0


LNU142
27541.1.
5.007
0.001
11
LNU130
24914.5.
7.654
0.394
11
LNU119
26141.1.
70.3
0.468
27


LNU142
27546.1.
4.599
0.429
2
LNU130
24913.6.
7.350
0.664
6
LNU119
26142.5.
59.0
0.586
7


LNU149
26175.3.
4.779
0.002
6
LNU136
14515.1.
7.530
0.508
9
LNU130
24913.5.
63.7
0.260
15


LNU15
14123.13.
4.878
0.001
8
LNU136
14511.10.
7.444
0.692
8
LNU130
24914.5.
61.2
0.394
11


LNU15
14123.11.
4.818
0.550
7
LNU136
14514.8.
7.389
0.728
7
LNU130
24913.6.
58.8
0.664
6


LNU185
26474.2.
5.052
0.503
12
LNU142
27546.2.
7.551
0.642
9
LNU136
14515.1.
60.2
0.508
9


LNU185
26475.1.
4.953
0.147
10
LNU142
27546.1.
7.487
0.504
8
LNU136
14511.10.
59.6
0.692
8


LNU185
26474.1.
4.889
0.530
8
LNU142
27545.1.
7.329
0.792
6
LNU136
14514.8.
59.1
0.728
7


LNU212
25834.4.
5.219
0.236
16
LNU149
26174.7.
7.171
0.788
4
LNU142
27546.2.
60.4
0.642
9


LNU212
25833.2.
5.176
0.153
15
LNU15
14124.12.
8.132
0.349
18
LNU142
27546.1.
59.9
0.504
8


LNU212
25834.5.
5.085
0.167
13
LNU15
14122.8.
8.027
0.486
16
LNU142
27545.1.
58.6
0.792
6


LNU212
25834.1.
4.707
0.624
4
LNU15
14123.13.
7.620
0.415
10
LNU149
26174.7.
57.4
0.788
4


LNU212
25832.1.
4.689
0.346
4
LNU185
26474.1.
8.921
0.055
29
LNU15
14124.12.
65.1
0.349
18


LNU216
25982.1.
5.253
0.053
16
LNU212
25834.4.
8.727
0.084
26
LNU15
14122.8.
64.2
0.486
16


LNU216
25985.4.
5.187
0.306
15
LNU212
25834.5.
8.061
0.217
17
LNU15
14123.13.
61.0
0.415
10


LNU216
25984.1.
5.160
0.310
14
LNU212
25833.2.
7.256
0.683
5
LNU185
26474.1.
71.4
0.055
29


LNU216
25984.6.
4.889
0.000
8
LNU216
25985.4.
9.450
0.146
37
LNU212
25834.4.
69.8
0.084
26


LNU228
26224.7.
5.104
0.000
13
LNU216
25982.1.
7.955
0.280
15
LNU212
25834.5.
64.5
0.217
17


LNU228
26222.4.
4.805
0.198
6
LNU216
25984.6.
7.667
0.701
11
LNU212
25833.2.
58.1
0.683
5


LNU229
26112.3.
4.981
0.000
10
LNU216
25982.2.
7.312
0.641
6
LNU216
25985.4.
75.6
0.146
37


LNU253
26241.1.
4.900
0.000
9
LNU228
26225.2.
9.276
0.044
34
LNU216
25982.1.
63.6
0.280
15


LNU253
26245.1.
4.580
0.372
1
LNU228
26224.7.
8.572
0.399
24
LNU216
25984.6.
61.3
0.701
11


LNU274
26264.2.
4.699
0.606
4
LNU228
26222.4.
8.339
0.137
21
LNU216
25982.2.
58.5
0.641
6


LNU280
26162.1.
5.073
0.376
12
LNU228
26222.1.
8.289
0.164
20
LNU228
26225.2.
74.2
0.044
34


LNU55
26015.1.
4.909
0.385
9
LNU228
26224.6.
8.203
0.319
19
LNU228
26222.4.
66.7
0.137
21


LNU81
26034.2.
4.657
0.745
3
LNU229
26112.4.
8.649
0.257
25
LNU228
26222.1.
66.3
0.164
20


LNU81
26034.3.
4.588
0.501
2
LNU229
26111.7.
8.573
0.438
24
LNU228
26224.6.
65.6
0.319
19


CONT.
—
4.514
—
0
LNU229
26111.5.
8.441
0.277
22
LNU228
26224.7.
58.7
0.616
6


LNU119
26141.1.
5.132
0.455
14
LNU229
26112.3.
8.225
0.344
19
LNU229
26112.4.
69.2
0.257
25


LNU119
26142.5.
4.717
0.517
4
LNU241
26232.4.
8.028
0.230
16
LNU229
26111.7.
68.6
0.438
24


LNU130
24913.5.
4.742
0.500
5
LNU241
26233.3.
7.906
0.658
14
LNU229
26111.5.
67.5
0.277
22


LNU130
24913.6.
4.712
0.574
4
LNU241
26234.1.
7.487
0.502
8
LNU229
26112.3.
65.8
0.344
19


LNU130
24914.5.
4.685
0.604
4
LNU253
26242.1.
7.550
0.652
9
LNU241
26232.4.
64.2
0.230
16


LNU136
14511.10.
4.806
0.556
6
LNU253
26241.1.
7.211
0.734
4
LNU241
26233.3.
63.2
0.658
14


LNU136
14514.8.
4.785
0.592
6
LNU274
26265.1.
7.782
0.634
13
LNU241
26234.1.
59.9
0.502
8


LNU136
14515.1.
4.727
0.548
5
LNU274
26262.2.
7.698
0.582
11
LNU253
26242.1.
60.4
0.652
9


LNU142
27546.2.
4.748
0.558
5
LNU280
26162.1.
8.526
0.144
23
LNU253
26241.1.
57.7
0.734
4


LNU142
27546.1.
4.712
0.515
4
LNU280
26164.4.
7.630
0.408
10
LNU274
26265.1.
62.3
0.634
13


LNU142
27545.1.
4.687
0.683
4
LNU55
26013.4.
7.698
0.433
11
LNU274
26262.2.
61.6
0.582
11


LNU15
14122.8.
4.959
0.461
10
LNU55
26015.1.
7.646
0.435
11
LNU280
26162.1.
68.2
0.144
23


LNU15
14123.13.
4.837
0.301
7
LNU55
26013.3.
7.634
0.782
10
LNU55
26013.4.
61.6
0.433
11


LNU15
14124.12.
4.773
0.572
6
LNU81
26031.10.
8.300
0.656
20
LNU55
26015.1.
61.2
0.435
11


LNU185
26474.1.
5.288
0.053
17
LNU81
26031.2.
7.932
0.410
15
LNU55
26013.3.
61.1
0.782
10


LNU185
26473.1.
4.624
0.722
2
CON-
—
5.130
—
0.0
LNU81
26031.10.
66.4
0.656
20







TROL











LNU212
25834.4.
5.158
0.069
14
LNU61
26134.4
6.167
0.034
20.22
LNU81
26031.2.
63.5
0.410
15


LNU212
25834.5.
4.908
0.226
9
LNU61
26135.3
5.575
0.338
8.68
CONT.
—
40.689
—
0.0


LNU212
25834.1.
4.682
0.593
4
CONT.
—
5.130
—
0.0
LNU61
26134.4
49.340
0.02
21.26


LNU216
25985.4.
5.308
0.120
18
LNU115
27584.2
5.790
<0.4
12.9
LNU61
26135.3
44.604
0.33
9.62


LNU216
25982.1.
4.804
0.348
6
LNU115
27586.2
6.387
<0.1
24.5
CONT.
—
40.689
—
0.0


LNU216
25982.2.
4.712
0.619
4
LNU115
27586.3
5.314
<0.7
3.6
LNU115
27584.2
46.316
<0.3
13.8


LNU216
25984.6.
4.658
0.782
3
LNU123
27721.1
5.768
<0.4
12.4
LNU115
27586.2
51.093
<0.1
25.6


LNU216
25984.1.
4.639
0.747
3
LNU123
27721.2
6.131
<0.4
19.5
LNU115
27586.3
42.513
<0.9
4.5


LNU228
26225.2.
5.360
0.060
19
LNU123
27722.1
6.229
<0.1
21.4
LNU123
27721.1
46.143
<0.3
13.4


LNU228
26224.7.
5.173
0.325
15
LNU123
27722.4
6.830
<0.1
33.1
LNU123
27721.1
49.044
<0.1
20.5


LNU228
26222.1.
5.005
0.185
11
LNU123
27724.1
5.298
<0.8
3.3
LNU123
27722.1
49.835
<0.1
22.5


LNU228
26224.6.
4.928
0.350
9
LNU123
27724.2
5.585
<0.6
8.9
LNU123
27722.4
54.642
<0.1
34.3


LNU228
26222.4.
4.759
0.423
5
LNU127
27601.2
6.811
<0.1
32.8
LNU123
27724.1
42.383
<0.9
4.2


LNU229
26111.7.
5.050
0.417
12
LNU127
27601.3
6.212
<0.1
21.1
LNU123
27724.2
44.676
<0.5
9.8


LNU229
26112.3.
5.016
0.412
11
LNU127
27601.4
7.362
<0.1
43.5
LNU127
27601.2
54.489
<0.1
33.9


LNU229
26112.4.
4.983
0.320
10
LNU127
27603.1
6.636
<0.1
29.3
LNU127
27601.3
49.696
<0.1
22.1


LNU229
26111.5.
4.884
0.337
8
LNU127
27603.5
6.058
<0.4
18.1
LNU127
27601.4
58.894
<0.1
44.7


LNU241
26233.3.
5.068
0.575
12
LNU134
29191.3
6.109
<0.4
19.1
LNU127
27603.1
53.084
<0.1
30.5


LNU241
26232.4.
5.012
0.163
11
LNU134
29191.6
5.673
<0.6
10.6
LNU127
27603.5
48.464
<0.3
19.1


LNU241
26234.1.
4.673
0.598
4
LNU134
29192.1
7.116
<0.1
38.7
LNU134
29191.3
48.868
<0.1
20.1


LNU253
26242.1.
4.850
0.331
7
LNU134
29193.2
5.921
<0.4
15.4
LNU134
29191.6
45.388
<0.5
11.5


LNU253
26241.1.
4.677
0.604
4
LNU190
27555.2
5.704
<0.6
11.2
LNU134
29192.1
56.931
<0.1
39.9


LNU274
26262.2.
4.923
0.343
9
LNU198
27731.2
5.550
<0.6
8.2
LNU134
29193.2
47.367
<0.3
16.4


LNU274
26265.1.
4.804
0.680
6
LNU198
27734.4
5.441
<0.8
6.1
LNU190
27555.2
45.636
<0.5
12.2


LNU280
26162.1.
5.140
0.227
14
LNU198
27735.4
5.535
<0.6
7.9
LNU198
27731.2
44.399
<0.5
9.1


LNU280
26164.4
4.779
0.510
6
LNU200
27991.2
5.736
<0.6
11.8
LNU198
27734.4
43.531
<0.7
7.0


LNU55
26013.3.
4.868
0.663
8
LNU200
27992.2
5.827
<0.4
13.6
LNU198
27735.4
44.277
<0.5
8.8


LNU55
26013.4.
4.795
0.535
6
LNU200
27992.3
6.394
<0.1
24.6
LNU200
27991.2
45.887
<0.5
12.8


LNU55
26015.1.
4.687
0.636
4
LNU200
27993.4
6.850
<0.1
33.5
LNU200
27992.2
46.620
<0.3
14.6


LNU81
26031.10.
4.970
0.569
10
LNU200
27994.3
6.408
<0.1
24.9
LNU200
27992.3
51.155
<0.1
25.7


LNU81
26031.2.
4.792
0.582
6
LNU217
28231.3
6.348
<0.1
23.7
LNU200
27993.4
54.797
<0.1
34.7


CONT.
—
4.055
—
0.0
LNU217
28234.1
6.165
<0.1
20.2
LNU200
27994.3
51.262
<0.1
26.0


LNU115
27584.2
4.302
<0.6
6.1
LNU244
28013.6
6.179
<0.1
20.4
LNU217
28231.3
50.780
<0.1
24.8


LNU115
27586.2
4.561
<0.1
12.5
LNU244
28013.8
6.185
<0.1
20.6
LNU217
28234.1
45.735
<0.5
12.4


LNU123
27721.1
4.234
<0.6
4.4
LNU244
28014.3
5.743
<0.6
11.9
LNU244
28013.6
49.429
<0.1
21.5


LNU123
27721.2
4.546
<0.1
12.1
LNU244
28014.4
6.687
<0.1
30.4
LNU244
28013.8
49.477
<0.1
21.6


LNU123
27722.1
4.427
0.25
9.2
LNU244
28015.1
6.208
<0.1
21.0
LNU244
28014.3
45.945
<0.5
12.9


LNU123
27722.4
4.726
<0.1
16.6
LNU262
27591.3
5.986
<0.4
16.7
LNU244
28014.4
53.499
<0.1
31.5


LNU123
27724.1
4.230
<0.6
4.3
LNU262
27591.7
6.332
<0.1
23.4
LNU244
28015.1
49.667
<0.1
22.1


LNU127
27601.2
4.661
<0.1
15.0
LNU262
27593.6
6.748
<0.1
31.5
LNU262
27591.3
47.887
<0.3
17.7


LNU127
27601.3
4.404
<0.25
8.6
LNU262
27595.4
6.770
<0.1
32.0
LNU262
27591.7
50.657
<0.1
24.5


LNU127
27601.4
4.689
<0.1
15.7
LNU266
27932.1
6.839
<0.1
33.3
LNU262
27593.6
53.987
<0.1
32.7


LNU127
27603.1
4.461
<0.25
10.0
LNU266
27935.3
5.920
<0.4
15.4
LNU262
27595.2
41.885
<0.9
2.9


LNU127
27603.5
4.477
<0.25
10.4
LNU266
27935.4
6.236
<0.1
21.6
LNU262
27595.4
54.162
<0.1
33.1


LNU134
29191.3
4.275
<0.6
5.4
LNU29
27651.3
5.393
<0.8
5.1
LNU266
27932.1
54.714
<0.1
34.5


LNU134
29192.1
4.774
<0.1
17.7
LNU29
27653.1
6.158
<0.1
20.0
LNU266
27935.3
47.363
<0.3
16.4


LNU190
27555.2
4.209
<0.7
3.8
LNU32
29251.1
5.634
<0.6
9.8
LNU266
27935.4
49.891
<0.1
22.6


LNU198
27734.4
4.216
<0.7
4.0
LNU32
29252.6
5.641
<0.6
10.0
LNU29
27651.3
43.145
<0.7
6.0


LNU200
27991.2
4.293
<0.6
5.9
LNU51
27611.1
5.810
<0.4
13.3
LNU29
27653.1
49.264
<0.1
21.1


LNU200
27992.2
4.337
<0.6
7.0
LNU51
27613.3
5.593
<0.6
9.0
LNU29
27654.1
41.748
<0.9
2.6


LNU200
27992.3
4.609
<0.1
13.7
LNU51
27614.2
5.505
<0.8
7.3
LNU32
29251.1
45.070
<0.5
10.8


LNU200
27993.4
4.754
<0.1
17.3
LNU58
27673.2
6.263
<0.1
22.1
LNU32
29252.6
45.129
<0.5
10.9


LNU200
27994.3
4.487
<0.25
10.7
LNU58
27673.3
5.560
<0.6
8.4
LNU51
27611.1
46.483
<0.3
14.2


LNU217
28231.3
4.431
<0.25
9.3
LNU58
27673.4
5.416
<0.8
5.6
LNU51
27613.3
44.746
<0.5
10.0


LNU217
28234.1
4.432
<0.25
9.3
LNU58
27673.6
5.456
<0.8
6.3
LNU51
27614.2
44.042
<0.5
8.2


LNU244
28013.6
4.470
<0.25
10.2
LNU58
27673.8
5.481
<0.8
6.8
LNU58
27673.2
50.103
<0.1
23.1


LNU244
28013.8
4.365
<0.6
7.6
CONT.
8252.24
1.222
—
0.0
LNU58
27673.3
44.483
<0.5
9.3


LNU244
28014.3
4.337
<0.6
7.0
LNU89
25325.1
1.521
0.03
24.5
LNU58
27673.4
43.332
<0.7
6.5


LNU244
28014.4
4.543
<0.1
12.0
LNU89
27193.2
1.438
0.11
17.7
LNU58
27673.8
43.851
<0.7
7.8


LNU244
28015.1
4.451
<0.25
9.8





CONT.
—
8.301 
—
0.0


LNU262
27591.3
4.358
<0.6
7.5





LNU89
25325.1
12.168
0.0005
46.6


LNU262
27591.7
4.642
<0.1
14.5





LNU89
27193.2
10.064
0.047
21.2


LNU262
27593.6
4.659
<0.1
14.9












LNU262
27595.4
4.664
<0.1
15.0












LNU266
27932.1
4.708
<0.1
16.1












LNU266
27935.3
4.412
<0.25
8.8












LNU266
27935.4
4.558
<0.1
12.4












LNU29
27653.1
4.643
<0.1
14.5












LNU32
29251.1
4.206
<0.7
3.7












LNU51
27614.2
4.177
<0.7
3.0












LNU58
27673.2
4.410
<0.25
8.8












LNU58
27673.3
4.331
<0.6
6.8












CONT.
8252.24
1.922
—
0.0












LNU89
25325.1
2.153
0.07
12.0












LNU89
27193.2
2.107
0.14
9.6





Table 79.


“CONT.”—Control;


“Ave.”—Average;


“% Incr.” = % increment.






The genes listed in Table 80 improved plant NUE when grown at limiting nitrogen concentration levels. These genes produced photosynthetic areas as seen by their larger leaf number, leaf blade area and petiole area. The genes were cloned under the regulation of a constitutive (At6669) and root preferred promoter (RootP). The evaluation of each gene was performed by testing the performance of different number of events. Event with p-value <0.1 was considered statistically significant.







TABLE 80







Genes showing improved plant photosynthetic capacity at limiting nitrogen growth conditions 




















Leaf Blade


Leaf Petiole




Leaf Number


Area [cm2]


Length [cm]





















Gene 


P- 
%
Gene 


P-
%
Gene 


P-
% 


Name
Event #
Ave.
Value
incr.
Name
Event #
Ave.
Value
incr.
Name
Event #
Ave.
Value
incr.
























CONT.
—
10.679
—
0
CONT.
—
0.634
—
0
CONT.
—
0.725
—
0


LNU100
14472.2
11.125
0.089
4
LNU100
14472.2
0.811
0.001
28
LNU100
14472.2
0.902
0.008
24


LNU100
14471.4
10.813
0.513
1
LNU100
14471.4
0.765
0.110
21
LNU100
14471.4
0.822
0.000
13


LNU104
25033.1
11.313
0.237
6
LNU100
14474.3
0.703
0.314
11
LNU100
14474.3
0.785
0.198
8


LNU104
25033.3
11.313
0.083
6
LNU100
14473.3
0.674
0.112
6
LNU100
14473.3
0.744
0.413
3


LNU104
25032.1
10.938
0.390
2
LNU104
25033.3
0.862
0.000
36
LNU104
25033.3
0.943
0.002
30


LNU106
14481.1
11.188
0.027
5
LNU104
25032.2
0.854
0.000
35
LNU104
25032.2
0.842
0.008
16


LNU106
14483.5
11.063
0.412
4
LNU104
25032.1
0.775
0.133
22
LNU104
25032.1
0.823
0.294
13


LNU114
25042.1
11.021
0.501
3
LNU104
25033.1
0.694
0.027
9
LNU104
25033.1
0.819
0.036
13


LNU155
14525.1
11.250
0.190
5
LNU104
25034.1
0.653
0.743
3
LNU104
25034.1
0.764
0.636
5


LNU155
14523.1
11.063
0.079
4
LNU106
14481.1
0.829
0.076
31
LNU106
14481.1
0.878
0.028
21


LNU218
24784.2
11.500
0.009
8
LNU106
14483.5
0.768
0.284
21
LNU106
14483.2
0.869
0.001
20


LNU218
24781.4
11.000
0.636
3
LNU106
14484.3
0.739
0.000
17
LNU106
14483.5
0.862
0.206
19


LNU218
24781.7
10.938
0.390
2
LNU106
14483.2
0.725
0.001
14
LNU106
14484.3
0.757
0.195
4


LNU218
24781.1
10.750
0.762
1
LNU114
25042.1
0.705
0.024
11
LNU114
25041.1
0.806
0.377
11


LNU23
25163.2
11.063
0.079
4
LNU114
25041.2
0.702
0.432
11
LNU114
25042.1
0.786
0.417
8


LNU23
25162.1
10.839
0.767
1
LNU114
25041.1
0.659
0.664
4
LNU114
25041.2
0.779
0.504
7


LNU23
25163.6
10.813
0.513
1
LNU155
14525.1
0.718
0.110
13
LNU155
14525.1
0.799
0.295
10


LNU28
25171.4
11.042
0.727
3
LNU155
14523.5
0.711
0.251
12
LNU155
14523.5
0.757
0.671
4


LNU28
25171.1
11.000
0.543
3
LNU213
24654.4
0.716
0.002
13
LNU213
24653.2
0.821
0.312
13


LNU28
25171.2
10.813
0.643
1
LNU218
24781.7
0.811
0.001
28
LNU213
24654.4
0.782
0.419
8


LNU4
25133.3
11.161
0.432
5
LNU218
24781.4
0.788
0.140
24
LNU218
24781.4
0.905
0.003
25


LNU40
24794.3
11.438
0.186
7
LNU218
24784.2
0.710
0.247
12
LNU218
24781.7
0.892
0.002
23


LNU40
24794.4
11.229
0.371
5
LNU218
24781.2
0.658
0.336
4
LNU218
24784.2
0.809
0.312
12


LNU40
24792.2
11.000
0.119
3
LNU23
25163.5
0.767
0.469
21
LNU218
24781.1
0.794
0.396
10


LNU46
14462.5
11.938
0.000
12
LNU23
25163.6
0.687
0.670
8
LNU23
25163.5
0.800
0.652
10


LNU46
14464.4
11.313
0.083
6
LNU23
25163.2
0.660
0.733
4
LNU23
25162.1
0.792
0.188
9


LNU46
14462.1
11.000
0.197
3
LNU28
25171.1
0.782
0.046
23
LNU23
25163.6
0.773
0.607
7


LNU46
14463.1
10.875
0.320
2
LNU28
25171.2
0.772
0.000
22
LNU23
25163.2
0.747
0.751
3


LNU48
24801.4
11.750
0.161
10
LNU28
25174.5
0.684
0.751
8
LNU28
25171.1
0.860
0.000
19


LNU48
24802.1
11.188
0.309
5
LNU4
25133.3
0.755
0.000
19
LNU28
25171.4
0.827
0.407
14


LNU48
24804.4
10.875
0.320
2
LNU4
25134.1
0.693
0.527
9
LNU28
25171.2
0.822
0.000
13


LNU63
24814.2
10.813
0.752
1
LNU4
25131.1
0.653
0.785
3
LNU28
25174.5
0.764
0.710
5


LNU8
25062.1
11.063
0.229
4
LNU40
24794.3
0.782
0.000
23
LNU4
25133.3
0.861
0.033
19


LNU8
25061.2
10.875
0.586
2
LNU40
24794.4
0.745
0.154
18
LNU4
25131.1
0.761
0.268
5


LNU8
25063.6
10.813
0.643
1
LNU46
14464.4
0.883
0.291
39
LNU4
25134.1
0.759
0.361
5


LNU94
24833.3
11.250
0.190
5
LNU46
14462.5
0.861
0.185
36
LNU40
24794.3
0.850
0.166
17


LNU96
25071.2
10.813
0.643
1
LNU46
14463.1
0.700
0.088
10
LNU40
24794.4
0.835
0.000
15


CONT.
—
10.798
—
0
LNU46
14464.1
0.695
0.200
10
LNU40
24792.1
0.754
0.372
4


LNU113
25631.1
11.563
0.204
7
LNU46
14462.1
0.666
0.666
5
LNU40
24792.2
0.746
0.307
3


LNU120
25464.1
11.375
0.628
5
LNU48
24801.4
0.790
0.000
25
LNU46
14462.5
0.963
0.219
33


LNU120
25463.6
11.188
0.219
4
LNU48
24802.1
0.691
0.018
9
LNU46
14464.4
0.905
0.299
25


LNU120
25463.3
10.938
0.375
1
LNU48
24804.4
0.667
0.178
5
LNU46
14463.1
0.796
0.003
10


LNU120
25463.7
10.929
0.419
1
LNU63
24814.2
0.756
0.000
19
LNU46
14464.1
0.766
0.049
6


LNU124
14502.1
11.188
0.025
4
LNU63
24814.3
0.696
0.020
10
LNU46
14462.1
0.762
0.584
5


LNU124
14502.7
11.000
0.756
2
LNU63
24811.2
0.693
0.025
9
LNU48
24802.1
0.878
0.000
21


LNU124
14501.1
10.875
0.699
1
LNU7
25081.1
0.722
0.002
14
LNU48
24801.4
0.853
0.000
18


LNU132
14102.6
11.125
0.148
3
LNU7
25082.2
0.694
0.150
9
LNU48
24803.2
0.733
0.701
1


LNU132
14102.7
11.000
0.564
2
LNU8
25063.6
0.693
0.345
9
LNU63
24814.2
0.810
0.002
12


LNU140
14115.1
11.250
0.527
4
LNU8
25062.1
0.679
0.076
7
LNU63
24814.3
0.797
0.003
10


LNU148
25685.6
11.875
0.000
10
LNU8
25062.2
0.649
0.460
2
LNU63
24811.2
0.745
0.671
3


LNU148
25685.1
11.563
0.067
7
LNU8
25061.2
0.643
0.650
2
LNU7
25081.1
0.849
0.000
17


LNU148
25685.9
11.375
0.032
5
LNU94
24833.3
0.714
0.054
13
LNU7
25082.2
0.812
0.082
12


LNU287
24674.6
11.000
0.686
2
LNU96
25071.2
0.801
0.130
26
LNU7
25083.3
0.770
0.598
6


LNU37
14061.7
11.063
0.547
2
LNU96
25073.4
0.701
0.694
11
LNU8
25063.6
0.792
0.013
9


LNU5
14043.7
11.063
0.721
2
LNU96
25071.3
0.681
0.268
8
LNU8
25061.2
0.765
0.536
5


LNU5
14043.9
10.938
0.736
1
CONT.
—
0.788
—
0
LNU8
25062.1
0.754
0.485
4


LNU72
24962.3
10.938
0.375
1
LNU113
25631.7
0.894
0.290
13
LNU94
24833.3
0.795
0.003
10


LNU74
25444.1
11.188
0.531
4
LNU113
25631.3
0.853
0.488
8
LNU96
25071.2
0.893
0.225
23


LNU74
25443.2
11.125
0.537
3
LNU140
14115.1
0.806
0.789
2
LNU96
25074.1
0.786
0.362
8


LNU87
24712.4
11.313
0.006
5
LNU148
25685.6
0.979
0.181
24
LNU96
25073.3
0.784
0.469
8


LNU87
24714.3
11.188
0.613
4
LNU148
25685.1
0.868
0.009
10
LNU96
25073.4
0.781
0.578
8


LNU98
25763.2
10.938
0.736
1
LNU5
14043.7
0.859
0.400
9
LNU96
25071.3
0.779
0.407
7


CONT.
—
10.726
—
0
LNU68
14035.5
0.816
0.192
4
CONT.
—
0.754
—
0


LNU113
25631.9
11.063
0.461
3
LNU74
25444.1
0.878
0.151
11
LNU113
25631.7
0.807
0.570
7


LNU113
25631.3
10.938
0.772
2
LNU74
25443.2
0.803
0.536
2
LNU113
25631.3
0.793
0.424
5


LNU120
25463.6
11.438
0.416
7
LNU98
25763.2
0.823
0.203
5
LNU113
25631.1
0.768
0.530
2


LNU124
14504.5
11.063
0.750
3
CONT.
—
0.712
—
0
LNU120
25463.3
0.763
0.796
1


LNU148
25685.1
11.375
0.021
6
LNU113
25631.9
0.783
0.320
10
LNU124
14501.1
0.782
0.372
4


LNU148
25685.2
10.938
0.218
2
LNU120
25463.3
0.734
0.786
3
LNU132
14102.6
0.794
0.132
5


LNU72
24962.3
11.188
0.164
4
LNU148
25685.1
0.771
0.122
8
LNU140
14115.1
0.770
0.380
2


LNU72
24963.7
11.000
0.737
3
LNU72
24963.7
0.789
0.543
11
LNU148
25685.6
0.845
0.270
12


LNU74
25443.5
11.000
0.219
3
LNU72
24962.3
0.740
0.793
4
LNU148
25685.1
0.835
0.191
11


CONT.
—
10.625
—
0
LNU98
25763.2
0.802
0.253
13
LNU287
24674.6
0.784
0.613
4


LNU117
25933.3
11.688
0.143
10
CONT.
—
0.692
—
0
LNU5
14043.7
0.783
0.413
4


LNU117
25931.4
11.125
0.398
5
LNU117
25931.4
1.085
0.072
57
LNU74
25443.3
0.794
0.491
5


LNU117
25932.4
11.063
0.436
4
LNU117
25931.2
0.951
0.036
37
LNU74
25444.1
0.767
0.671
2


LNU117
25931.2
10.875
0.632
2
LNU117
25933.3
0.927
0.043
34
LNU74
25443.2
0.759
0.757
1


LNU122
25333.2
12.188
0.068
15
LNU117
25931.1
0.897
0.110
30
LNU87
24713.2
0.780
0.758
3


LNU122
25332.5
11.375
0.225
7
LNU117
25932.4
0.841
0.457
22
LNU98
25763.2
0.775
0.386
3


LNU122
25333.1
11.375
0.225
7
LNU122
25332.1
0.985
0.158
42
CONT.
—
0.753
—
0


LNU122
25332.1
11.250
0.307
6
LNU122
25333.2
0.959
0.030
39
LNU113
25631.9
0.794
0.664
5


LNU122
25332.2
11.000
0.483
4
LNU122
25332.2
0.842
0.096
22
LNU148
25685.1
0.781
0.655
4


LNU125
25941.4
12.063
0.114
14
LNU122
25332.5
0.837
0.092
21
LNU72
24962.3
0.793
0.677
5


LNU125
25941.2
11.625
0.150
9
LNU122
25333.1
0.796
0.383
15
LNU98
25763.2
0.794
0.125
5


LNU125
25943.3
11.313
0.256
6
LNU125
25941.4
1.017
0.107
47
CONT.
—
0.663
—
0


LNU125
25943.2
10.875
0.738
2
LNU125
25943.3
0.935
0.027
35
LNU117
25931.4
0.906
0.055
37


LNU125
25944.3
10.813
0.724
2
LNU125
25943.2
0.918
0.310
33
LNU117
25933.3
0.845
0.077
27


LNU138
14074.6
11.438
0.200
8
LNU125
25941.2
0.814
0.516
18
LNU117
25932.4
0.812
0.161
23


LNU138
14074.5
11.375
0.230
7
LNU125
25944.3
0.811
0.427
17
LNU117
25931.2
0.809
0.121
22


LNU138
14071.5
10.875
0.695
2
LNU138
14074.5
1.033
0.015
49
LNU117
25931.1
0.745
0.545
12


LNU180
24721.4
12.000
0.075
13
LNU38
14074.6
0.962
0.273
39
LNU122
25333.2
0.878
0.054
32


LNU180
24722.2
11.750
0.124
11
LNU38
14071.5
0.904
0.041
31
LNU122
25332.1
0.821
0.124
24


LNU180
24724.1
11.750
0.124
11
LNU38
14072.5
0.714
0.781
3
LNU122
25333.1
0.792
0.169
19


LNU180
24721.2
11.563
0.180
9
LNU80
24721.2
1.181
0.022
71
LNU122
25332.5
0.772
0.278
17


LNU180
24723.1
11.375
0.230
7
LNU80
24723.1
1.124
0.008
62
LNU122
25332.2
0.751
0.385
13


LNU220
25405.5
11.313
0.258
6
LNU80
24721.4
1.097
0.116
59
LNU125
25941.4
0.885
0.230
33


LNU220
25405.1
11.250
0.361
6
LNU80
24722.2
1.065
0.090
54
LNU125
25941.2
0.817
0.141
23


LNU220
25405.2
11.125
0.398
5
LNU80
24724.1
0.963
0.058
39
LNU125
25943.2
0.799
0.269
21


LNU220
25405.6
11.000
0.513
4
LNU220
25405.2
1.026
0.043
48
LNU125
25943.3
0.795
0.181
20


LNU220
25405.3
10.813
0.724
2
LNU220
25405.5
1.019
0.106
47
LNU125
25944.3
0.768
0.253
16


LNU230
25415.1
11.750
0.150
11
LNU220
25405.1
1.011
0.233
46
LNU138
14074.5
0.867
0.075
31


LNU230
25413.2
11.500
0.537
8
LNU220
25405.6
0.889
0.048
29
LNU138
14074.6
0.851
0.104
28


LNU230
25413.1
11.313
0.495
6
LNU220
25405.3
0.802
0.515
16
LNU138
14071.5
0.791
0.165
19


LNU230
25412.2
11.277
0.274
6
LNU230
25413.1
1.105
0.109
60
LNU138
14072.8
0.737
0.338
11


LNU230
25412.1
11.188
0.328
5
LNU230
25412.1
1.014
0.101
46
LNU180
24721.2
0.952
0.026
44


LNU234
25014.6
11.313
0.258
6
LNU230
25415.1
0.966
0.211
40
LNU180
24722.2
0.918
0.037
38


LNU234
25014.5
11.063
0.598
4
LNU230
25413.2
0.911
0.239
32
LNU180
24723.1
0.914
0.051
38


LNU234
25014.1
10.938
0.552
3
LNU230
25412.2
0.891
0.048
29
LNU180
24721.4
0.889
0.055
34


LNU234
25014.4
10.938
0.566
3
LNU234
25014.8
1.020
0.016
47
LNU180
24724.1
0.858
0.107
29


LNU25
14082.9
11.688
0.134
10
LNU234
25014.1
0.930
0.131
34
LNU220
25405.1
0.822
0.179
24


LNU25
14083.1
11.625
0.321
9
LNU234
25014.6
0.843
0.090
22
LNU220
25405.5
0.808
0.171
22


LNU25
14084.6
11.500
0.230
8
LNU234
25014.4
0.834
0.101
20
LNU220
25405.2
0.767
0.217
16


LNU25
14082.8
11.000
0.488
4
LNU234
25014.5
0.815
0.435
18
LNU220
25405.6
0.746
0.441
12


LNU254
25781.3
11.625
0.143
9
LNU25
14083.7
1.052
0.010
52
LNU220
25405.3
0.739
0.385
11


LNU254
25781.5
11.625
0.145
9
LNU25
14082.8
1.052
0.103
52
LNU230
25412.1
0.896
0.045
35


LNU254
25782.5
11.500
0.299
8
LNU25
14082.9
0.982
0.022
42
LNU230
25413.1
0.856
0.073
29


LNU254
25783.1
11.313
0.258
6
LNU25
14084.6
0.981
0.399
42
LNU230
25412.2
0.807
0.133
22


LNU254
25782.4
11.125
0.337
5
LNU25
14083.1
0.973
0.028
41
LNU230
25415.1
0.775
0.349
17


LNU263
25794.6
11.813
0.101
11
LNU254
25782.4
1.061
0.014
53
LNU230
25413.2
0.772
0.438
16


LNU263
25794.8
11.500
0.185
8
LNU254
25781.3
0.995
0.022
44
LNU234
25014.8
0.872
0.123
31


LNU263
25792.2
11.188
0.373
5
LNU254
25781.5
0.956
0.029
38
LNU234
25014.1
0.856
0.319
29


LNU263
25791.3
11.125
0.452
5
LNU254
25783.1
0.868
0.371
25
LNU234
25014.6
0.815
0.113
23


LNU263
25794.3
11.063
0.476
4
LNU254
25782.5
0.828
0.199
20
LNU234
25014.5
0.780
0.359
18


LNU267
25804.3
11.250
0.307
6
LNU263
25791.3
1.060
0.056
53
LNU234
25014.4
0.762
0.283
15


LNU267
25803.1
11.188
0.373
5
LNU263
25794.8
1.042
0.269
51
LNU25
14082.8
0.932
0.040
41


LNU267
25804.4
11.125
0.452
5
LNU263
25794.3
1.015
0.013
47
LNU25
14082.9
0.920
0.035
39


LNU267
25802.1
10.813
0.751
2
LNU263
25794.6
1.003
0.032
45
LNU25
14083.7
0.901
0.173
36


LNU271
25912.2
11.563
0.164
9
LNU263
25792.2
0.934
0.058
35
LNU25
14084.6
0.896
0.145
35


LNU271
25911.4
11.500
0.178
8
LNU267
25804.4
0.986
0.021
43
LNU25
14083.1
0.890
0.047
34


LNU271
25913.2
11.250
0.361
6
LNU267
25804.3
0.959
0.265
39
LNU254
25782.4
0.870
0.067
31


LNU271
25912.1
11.161
0.539
5
LNU267
25801.1
0.938
0.107
36
LNU254
25781.3
0.859
0.079
30


LNU271
25913.3
10.813
0.751
2
LNU267
25803.1
0.904
0.040
31
LNU254
25783.1
0.832
0.227
26


LNU278
25814.3
11.438
0.200
8
LNU267
25802.1
0.877
0.188
27
LNU254
25782.5
0.825
0.101
24


LNU278
25812.3
11.375
0.237
7
LNU271
25911.4
1.163
0.010
68
LNU254
25781.5
0.819
0.118
23


LNU278
25813.2
11.250
0.289
6
LNU271
25912.1
1.012
0.014
46
LNU263
25791.3
0.911
0.043
37


LNU278
25812.2
11.063
0.436
4
LNU271
25913.3
0.909
0.053
31
LNU263
25794.6
0.892
0.892
34


LNU36
25562.3
11.188
0.328
5
LNU271
25912.2
0.796
0.173
15
LNU263
25794.8
0.866
0.866
31


LNU36
25562.7
11.188
0.658
5
LNU278
25812.3
1.100
0.012
59
LNU263
25794.3
0.859
0.859
30


LNU36
25561.2
11.063
0.436
4
LNU278
25814.3
1.093
0.010
58
LNU263
25792.2
0.815
0.121
23


LNU43
14423.6
11.688
0.125
10
LNU278
25814.1
0.901
0.155
30
LNU267
25803.1
0.812
0.128
22


LNU43
14422.9
11.313
0.256
6
LNU278
25812.2
0.882
0.072
28
LNU267
25804.4
0.809
0.266
22


LNU43
14421.1
11.188
0.334
5
LNU278
25813.2
0.777
0.319
12
LNU267
25804.3
0.808
0.250
22


LNU43
14422.8
11.000
0.621
4
LNU36
25562.9
1.095
0.021
58
LNU267
25801.1
0.760
0.238
15


LNU45
25052.12
11.688
0.143
10
LNU36
25562.3
0.896
0.048
30
LNU267
25802.1
0.747
0.299
13


LNU45
25052.9
11.500
0.178
8
LNU36
25562.4
0.884
0.048
28
LNU271
25911.4
0.953
0.035
44


LNU45
25053.4
11.375
0.237
7
LNU36
25562.7
0.874
0.101
26
LNU271
25912.1
0.861
0.094
30


LNU45
25052.11
11.188
0.328
5
LNU36
25561.2
0.752
0.400
9
LNU271
25913.3
0.794
0.162
20


LNU45
25052.8
10.813
0.712
2
LNU43
14422.8
1.015
0.022
47
LNU271
25912.2
0.775
0.197
17


LNU67
25821.5
11.250
0.361
6
LNU43
14422.9
0.941
0.033
36
LNU271
25913.2
0.744
0.382
12


LNU67
25824.5
10.875
0.656
2
LNU43
14421.1
0.912
0.044
32
LNU278
25812.3
0.892
0.057
35


LNU67
25821.4
10.813
0.784
2
LNU43
14423.7
0.859
0.267
24
LNU278
25814.3
0.816
0.122
23


CONT.
—
10.125
—
0
LNU43
14423.6
0.833
0.125
20
LNU278
25812.2
0.783
0.172
18


LNU100
14474.2
11.063
0.319
9
LNU45
25052.8
0.979
0.026
41
LNU278
25814.1
0.776
0.192
17


LNU100
14472.1
10.813
0.065
7
LNU45
25052.12
0.957
0.172
38
LNU278
25813.2
0.744
0.312
12


LNU100
14473.3
10.625
0.381
5
LNU45
25052.9
0.920
0.062
33
LNU36
25562.9
0.862
0.069
30


LNU100
14473.1
10.500
0.487
4
LNU45
25053.4
0.871
0.507
26
LNU36
25561.2
0.796
0.147
20


LNU104
25033.1
10.750
0.033
6
LNU45
25052.11
0.755
0.339
9
LNU36
25562.7
0.735
0.346
11


LNU104
25032.1
10.688
0.490
6
LNU67
25823.5
0.875
0.062
26
LNU36
25562.3
0.719
0.445
8


LNU104
25032.2
10.688
0.655
6
LNU67
25824.5
0.871
0.368
26
LNU36
25562.4
0.686
0.744
3


LNU104
25033.3
10.625
0.037
5
LNU67
25821.5
0.864
0.355
25
LNU43
14421.1
0.890
0.047
34


LNU106
14483.2
11.000
0.300
9
LNU67
25821.4
0.830
0.175
20
LNU43
14422.8
0.853
0.075
29


LNU106
14483.5
10.813
0.009
7
LNU67
25824.3
0.752
0.732
9
LNU43
14422.9
0.845
0.091
27


LNU106
14482.3
10.375
0.502
2
CONT.
—
0.585
—
0
LNU43
14423.6
0.786
0.172
19


LNU106
14481.1
10.313
0.797
2
LNU100
14474.2
0.838
0.093
43
LNU43
14423.7
0.746
0.296
13


LNU106
14484.3
10.250
0.619
1
LNU100
14472.1
0.812
0.381
39
LNU45
25052.12
0.902
0.052
36


LNU114
25041.2
10.700
0.247
6
LNU100
14473.3
0.753
0.372
29
LNU45
25052.8
0.818
0.119
23


LNU114
25044.4
10.625
0.624
5
LNU100
14473.1
0.750
0.198
28
LNU45
25053.4
0.813
0.204
23


LNU114
25042.1
10.250
0.728
1
LNU100
14471.4
0.698
0.385
19
LNU45
25052.9
0.796
0.153
20


LNU117
25931.4
10.500
0.160
4
LNU104
25032.2
0.686
0.492
17
LNU45
25052.11
0.749
0.283
13


LNU117
25931.2
10.438
0.767
3
LNU104
25033.3
0.668
0.006
14
LNU67
25821.5
0.774
0.285
17


LNU117
25932.4
10.438
0.603
3
LNU104
25032.1
0.655
0.457
12
LNU67
25823.5
0.772
0.210
17


LNU155
14523.5
10.563
0.188
4
LNU104
25033.1
0.648
0.408
11
LNU67
25824.5
0.767
0.395
16


LNU180
24723.1
10.250
0.728
1
LNU106
14483.2
0.845
0.000
44
LNU67
25824.3
0.740
0.351
12


LNU182
24781.4
10.563
0.488
4
LNU106
14481.1
0.753
0.355
29
LNU67
25821.4
0.698
0.610
5


LNU254
25782.4
10.563
0.188
4
LNU106
14483.5
0.711
0.001
21
CONT.
—
0.683
—
0


LNU4
25134.3
10.938
0.003
8
LNU106
14484.3
0.664
0.541
13
LNU100
14473.3
0.874
0.197
28


LNU4
25134.2
10.875
0.244
7
LNU114
25044.11
0.787
0.364
34
LNU100
14474.2
0.827
0.155
21


LNU40
24794.4
10.375
0.256
2
LNU114
25042.1
0.718
0.140
23
LNU100
14472.1
0.806
0.320
18


LNU40
24792.1
10.313
0.665
2
LNU114
25041.2
0.716
0.100
22
LNU100
14473.1
0.756
0.005
11


LNU46
14462.5
10.750
0.033
6
LNU114
25041.1
0.658
0.757
12
LNU104
25033.1
0.862
0.461
26


LNU46
14464.4
10.625
0.073
5
LNU117
25932.4
0.791
0.009
35
LNU104
25033.3
0.826
0.197
21


LNU46
14462.1
10.313
0.797
2
LNU117
25931.4
0.725
0.025
24
LNU104
25032.2
0.766
0.309
12


LNU48
24801.4
10.813
0.328
7
LNU117
25931.1
0.693
0.592
18
LNU104
25032.1
0.758
0.594
11


LNU48
24803.2
10.313
0.797
2
LNU117
25931.2
0.633
0.340
8
LNU106
14483.2
0.865
0.175
27


LNU36
24812.3
10.500
0.487
4
LNU117
25933.3
0.613
0.731
5
LNU106
14481.1
0.849
0.409
24


LNU7
25082.2
10.625
0.037
5
LNU155
14523.5
0.741
0.313
27
LNU106
14483.5
0.751
0.017
10


LNU8
25061.2
10.813
0.009
7
LNU155
14524.8
0.630
0.220
8
LNU106
14484.3
0.710
0.741
4


LNU8
25063.6
10.688
0.490
6
LNU180
24723.1
0.755
0.062
29
LNU114
25044.11
0.864
0.115
27


LNU94
24833.3
10.375
0.627
2
LNU180
24722.2
0.641
0.737
10
LNU114
25041.2
0.788
0.001
15


CONT.
—
9.886
—
0
LNU218
24781.4
0.780
0.088
33
LNU114
25044.4
0.743
0.698
9


LNU122
25332.2
10.250
0.130
4
LNU218
24781.1
0.712
0.255
22
LNU114
25041.1
0.731
0.649
7


LNU122
25332.5
9.938
0.775
1
LNU218
24781.6
0.631
0.212
8
LNU114
25042.1
0.727
0.336
6


LNU125
25944.1
10.188
0.113
3
LNU218
24781.2
0.615
0.328
5
LNU117
25932.4
0.849
0.304
24


LNU220
25405.1
10.000
0.740
1
LNU254
25782.5
0.723
0.169
24
LNU117
25931.4
0.788
0.149
15


LNU236
25422.4
10.375
0.244
5
LNU4
25134.3
0.798
0.251
36
LNU117
25931.2
0.732
0.038
7


LNU236
25425.4
9.938
0.775
1
LNU4
25131.1
0.699
0.398
19
LNU117
25931.1
0.711
0.466
4


LNU45
25052.11
10.188
0.113
3
LNU4
25134.2
0.673
0.270
15
LNU155
14523.5
0.785
0.420
15


LNU45
25052.12
10.125
0.508
2
LNU4
25133.3
0.647
0.479
11
LNU180
24723.1
0.812
0.294
19


LNU45
25053.4
10.000
0.740
1
LNU40
24794.3
0.687
0.571
17
LNU180
24722.2
0.725
0.790
6


CONT.
—
10.528
—
0
LNU40
24792.1
0.683
0.015
17
LNU218
24781.1
0.844
0.000
24


LNU10
25123.5
11.438
0.233
9
LNU40
24794.4
0.631
0.149
8
LNU218
24781.4
0.834
0.270
22


LNU10
25123.6
11.063
0.063
5
LNU46
14462.5
0.746
0.301
27
LNU218
24781.2
0.746
0.039
9


LNU157
24982.8
11.250
0.018
7
LNU46
14462.1
0.653
0.087
12
LNU254
25782.5
0.738
0.022
8


LNU173
25451.1
11.375
0.504
8
LNU46
14464.4
0.615
0.486
5
LNU4
25134.3
0.841
0.486
23


LNU173
25451.5
10.688
0.624
2
LNU48
24801.4
0.694
0.254
19
LNU4
25134.2
0.743
0.463
9


LNU178
14611.5
11.500
0.004
9
LNU48
24803.2
0.666
0.605
14
LNU4
25133.3
0.705
0.354
3


LNU178
14611.4
11.313
0.445
7
LNU63
24812.3
0.777
0.125
33
LNU40
24792.1
0.852
0.011
25


LNU178
14611.1
11.250
0.557
7
LNU63
24814.7
0.649
0.437
11
LNU40
24794.3
0.786
0.579
15


LNU184
25393.2
11.125
0.505
6
LNU63
24814.2
0.603
0.689
3
LNU40
24793.1
0.696
0.779
2


LNU184
25393.3
10.688
0.719
2
LNU7
25082.2
0.713
0.441
22
LNU40
24794.4
0.694
0.703
2


LNU230
25413.1
11.625
0.002
10
LNU7
25083.1
0.678
0.034
16
LNU46
14462.5
0.860
0.259
26


LNU230
25413.2
11.625
0.226
10
LNU7
25083.3
0.642
0.070
10
LNU46
14464.4
0.714
0.544
5


LNU230
25415.1
11.125
0.004
6
LNU8
25063.6
0.779
0.138
33
LNU48
24803.2
0.804
0.393
18


LNU230
25412.1
11.063
0.063
5
LNU8
25061.2
0.682
0.002
16
LNU48
24804.4
0.770
0.443
13


LNU230
25412.2
10.813
0.787
3
LNU94
24833.3
0.696
0.405
19
LNU48
24801.4
0.735
0.573
8


LNU236
25422.4
11.188
0.081
6
CONT.
—
0.766
—
0
LNU63
24812.3
0.848
0.008
24


LNU236
25425.4
11.063
0.063
5
LNU10
25123.5
0.818
0.561
7
LNU63
24814.2
0.758
0.189
11


LNU236
25423.3
10.938
0.237
4
LNU122
25332.1
0.907
0.025
18
LNU63
24814.7
0.745
0.616
9


LNU236
25424.2
10.813
0.288
3
LNU122
25332.2
0.884
0.168
15
LNU7
25082.2
0.754
0.399
10


LNU24
24974.2
11.000
0.122
4
LNU122
25332.5
0.835
0.623
9
LNU7
25083.3
0.736
0.035
8


LNU24
24971.2
10.813
0.394
3
LNU125
25943.2
0.822
0.518
7
LNU7
25083.1
0.736
0.328
8


LNU24
24972.1
10.688
0.785
2
LNU125
25941.4
0.814
0.156
6
LNU8
25063.6
0.778
0.001
14


LNU263
25792.2
11.188
0.338
6
LNU138
14074.5
0.860
0.075
12
LNU8
25061.2
0.704
0.537
3


LNU263
25794.3
11.063
0.139
5
LNU138
14071.5
0.807
0.136
5
LNU94
24833.3
0.705
0.723
3


LNU263
25791.3
10.750
0.669
2
LNU138
14074.6
0.782
0.570
2
CONT.
—
0.808
—
0


LNU276
25431.1
11.438
0.006
9
LNU157
24982.1
0.831
0.413
8
LNU122
25332.1
0.856
0.240
6


LNU276
25433.3
10.750
0.388
2
LNU178
14614.5
0.896
0.071
17
LNU122
25332.2
0.844
0.239
5


LNU276
25433.1
10.688
0.624
2
LNU178
14612.1
0.826
0.469
8
LNU122
25332.5
0.830
0.586
3


LNU279
25481.4
10.813
0.288
3
LNU178
14611.5
0.824
0.042
8
LNU138
14071.5
0.851
0.003
5


LNU279
25484.3
10.750
0.565
2
LNU178
14611.4
0.824
0.040
8
LNU138
14074.6
0.821
0.726
2


LNU36
25562.7
11.250
0.119
7
LNU220
25405.6
0.875
0.048
14
LNU178
14614.5
0.864
0.384
7


LNU36
25562.3
10.777
0.613
2
LNU220
25405.5
0.874
0.028
14
LNU178
14611.5
0.848
0.005
5


LNU36
25561.2
10.750
0.565
2
LNU220
25405.1
0.835
0.086
9
LNU234
25014.6
0.828
0.146
3


LNU56
24693.1
10.875
0.725
3
LNU220
25405.2
0.823
0.324
7
LNU236
25424.2
0.936
0.000
16


LNU56
24694.2
10.688
0.785
2
LNU234
25014.4
0.848
0.669
11
LNU236
25425.4
0.865
0.308
7


LNU73
25755.1
11.375
0.083
8
LNU234
25014.5
0.806
0.678
5
LNU236
25423.3
0.857
0.623
6


LNU73
25751.1
11.125
0.505
6
LNU236
25424.2
0.980
0.000
28
LNU236
25422.4
0.827
0.683
2


LNU73
25751.8
10.938
0.392
4
LNU236
25425.4
0.868
0.476
13
LNU25
14082.8
0.897
0.036
11


LNU73
25751.9
10.688
0.624
2
LNU236
25423.3
0.868
0.428
13
LNU25
14082.9
0.848
0.438
5


LNU9
25001.2
11.063
0.414
5
LNU236
25422.4
0.843
0.244
10
LNU267
25804.4
0.823
0.592
2


LNU9
25001.7
10.875
0.613
3
LNU24
24974.2
0.821
0.216
7
LNU271
25911.4
0.830
0.124
3


LNU9
25001.1
10.813
0.288
3
LNU24
24972.1
0.800
0.590
4
LNU271
25912.1
0.824
0.783
2


CONT.
—
8.400
—
0
LNU25
14082.8
0.954
0.000
25
LNU278
25812.2
0.815
0.595
1


LNU131
14005.5
9.750
0.071
16
LNU25
14082.9
0.847
0.009
11
LNU43
14422.9
0.842
0.585
4


LNU131
14002.15
9.438
0.000
12
LNU25
14084.6
0.840
0.043
10
LNU43
14423.7
0.814
0.754
1


LNU131
14005.2
9.188
0.208
9
LNU25
14083.7
0.797
0.375
4
LNU45
25052.12
0.935
0.030
16


LNU135
26204.2
9.750
0.211
16
LNU267
25804.4
0.796
0.733
4
LNU45
25052.11
0.905
0.000
12


LNU135
26203.1
9.313
0.000
11
LNU271
25911.4
0.855
0.666
12
LNU45
25053.4
0.856
0.604
6


LNU135
26203.6
9.125
0.000
9
LNU271
25912.1
0.794
0.774
4
LNU45
25052.9
0.833
0.049
3


LNU135
26203.3
8.750
0.609
4
LNU278
25814.1
0.877
0.012
14
LNU67
25824.3
0.853
0.105
6


LNU161
14553.5
9.500
0.094
13
LNU278
25814.3
0.843
0.027
10
LNU67
25824.5
0.842
0.011
4


LNU161
14553.6
8.750
0.369
4
LNU278
25812.2
0.816
0.071
7
LNU67
25821.4
0.822
0.706
2


LNU173
25451.1
8.813
0.513
5
LNU278
25813.2
0.789
0.649
3
LNU9
25001.1
0.870
0.249
8


LNU173
25451.2
8.563
0.237
2
LNU43
14423.7
0.873
0.014
14
LNU9
25001.7
0.831
0.059
3


LNU173
25451.5
8.438
0.776
0
LNU43
14422.9
0.815
0.697
6
CONT.
—
0.804
—
0


LNU181
25771.2
9.250
0.327
10
LNU45
25052.12
1.036
0.000
35
LNU10
25123.6
0.832
0.506
4


LNU181
25774.1
9.188
0.077
9
LNU45
25053.4
0.932
0.327
22
LNU157
24982.8
0.901
0.119
12


LNU181
25771.6
9.063
0.000
8
LNU45
25052.9
0.856
0.031
12
LNU157
24982.4
0.885
0.385
10


LNU181
25771.11
8.750
0.515
4
LNU45
25052.11
0.831
0.448
9
LNU157
24983.3
0.852
0.542
6


LNU184
25394.3
9.688
0.000
15
LNU67
25824.3
0.949
0.000
24
LNU173
25451.1
0.884
0.386
10


LNU184
25393.3
9.313
0.268
11
LNU67
25824.5
0.889
0.001
16
LNU173
25451.11
0.817
0.776
2


LNU184
25395.1
9.250
0.507
10
LNU67
25821.4
0.845
0.196
10
LNU178
14611.4
0.962
0.394
20


LNU184
25394.1
9.125
0.375
9
LNU9
25001.7
0.910
0.345
19
LNU178
14611.1
0.906
0.339
13


LNU184
25393.1
8.813
0.395
5
LNU9
25001.1
0.896
0.149
17
LNU178
14611.5
0.843
0.669
5


LNU224
25874.1
9.875
0.133
18
LNU9
25003.1
0.830
0.544
8
LNU184
25393.3
0.906
0.385
13


LNU224
25872.3
9.375
0.009
12
LNU9
25001.3
0.792
0.359
3
LNU184
25393.2
0.864
0.196
7


LNU224
25871.3
9.188
0.208
9
LNU9
25001.2
0.782
0.798
2
LNU184
25395.1
0.820
0.703
2


LNU224
25874.4
9.063
0.251
8
CONT.
—
0.791
—
0
LNU230
25413.2
0.942
0.260
17


LNU224
25872.2
8.875
0.270
6
LNU10
25123.6
0.909
0.068
15
LNU230
25413.1
0.914
0.384
14


LNU246
25743.2
9.688
0.251
15
LNU157
24982.8
0.994
0.003
26
LNU230
25415.1
0.850
0.299
6


LNU246
25744.2
9.250
0.327
10
LNU157
24982.4
0.887
0.133
12
LNU230
25412.2
0.849
0.332
6


LNU246
25744.4
9.250
0.327
10
LNU157
24983.3
0.865
0.270
9
LNU230
25412.1
0.843
0.419
5


LNU246
25744.3
9.188
0.208
9
LNU168
24751.2
0.836
0.791
6
LNU236
25425.4
0.949
0.007
18


LNU246
25743.1
8.938
0.002
6
LNU173
25451.1
0.853
0.607
8
LNU236
25423.3
0.850
0.558
6


LNU250
25592.2
9.188
0.077
9
LNU178
14611.4
1.015
0.297
28
LNU24
24974.2
0.928
0.016
15


LNU250
25591.1
8.750
0.008
4
LNU178
14611.1
0.964
0.288
22
LNU24
24971.3
0.893
0.066
11


LNU250
25592.1
8.750
0.609
4
LNU178
14611.5
0.954
0.156
21
LNU24
24971.4
0.878
0.718
9


LNU250
25591.3
8.563
0.774
2
LNU184
25393.2
0.975
0.005
23
LNU24
24971.2
0.818
0.758
2


LNU250
25594.1
8.563
0.695
2
LNU184
25393.3
0.939
0.361
19
LNU263
25791.3
0.913
0.118
14


LNU260
26404.8
9.125
0.163
9
LNU184
25393.1
0.914
0.620
16
LNU263
25794.8
0.882
0.199
10


LNU260
26404.1
9.000
0.510
7
LNU184
25395.1
0.887
0.517
12
LNU263
25794.6
0.858
0.757
7


LNU260
26404.7
8.938
0.426
6
LNU184
25394.3
0.819
0.705
4
LNU263
25794.3
0.854
0.301
6


LNU260
26403.1
8.750
0.124
4
LNU230
25413.1
0.972
0.024
23
LNU276
25431.1
0.878
0.174
9


LNU276
25433.6
10.000
0.056
19
LNU230
25413.2
0.953
0.221
20
LNU276
25433.1
0.837
0.547
4


LNU276
25433.1
8.938
0.002
6
LNU230
25412.1
0.922
0.035
17
LNU279
25481.3
0.906
0.317
13


LNU276
25431.1
8.813
0.595
5
LNU230
25412.2
0.916
0.204
16
LNU279
25481.4
0.889
0.207
10


LNU276
25433.5
8.438
0.776
0
LNU230
25415.1
0.891
0.225
13
LNU279
25484.3
0.852
0.277
6


LNU279
25484.3
9.500
0.258
13
LNU236
25425.4
1.093
0.001
38
LNU279
25481.2
0.829
0.799
3


LNU279
25481.5
9.000
0.338
7
LNU236
25423.3
0.973
0.352
23
LNU36
25562.3
0.988
0.131
23


LNU279
25481.4
8.875
0.062
6
LNU236
25422.4
0.885
0.262
12
LNU36
25561.2
0.930
0.306
16


LNU279
25481.3
8.688
0.628
3
LNU236
25424.2
0.885
0.093
12
LNU36
25562.7
0.856
0.749
6


LNU3
26124.3
8.875
0.062
6
LNU24
24974.2
1.048
0.032
32
LNU53
25674.1
0.826
0.614
3


LNU3
26122.2
8.750
0.515
4
LNU24
24971.3
0.905
0.200
14
LNU56
24694.1
0.940
0.502
17


LNU33
25553.2
9.107
0.447
8
LNU263
25791.3
1.005
0.067
27
LNU56
24693.1
0.910
0.267
13


LNU33
25553.3
9.63
0.000
8
LNU263
25794.3
0.951
0.177
20
LNU56
24691.2
0.862
0.484
7


LNU33
25552.2
9.000
0.000
7
LNU263
25794.8
0.897
0.286
13
LNU56
24694.2
0.861
0.547
7


LNU53
25674.6
8.563
0.695
2
LNU263
25792.2
0.843
0.326
7
LNU73
25755.1
1.002
0.062
25


LNU56
24694.2
8.813
0.210
5
LNU276
25433.1
0.972
0.193
23
LNU73
25751.9
0.974
0.003
21


LNU56
24693.1
8.750
0.515
4
LNU276
25431.1
0.920
0.211
16
LNU73
25751.8
0.962
0.259
20


LNU73
25751.9
8.500
0.592
1
LNU276
25433.3
0.891
0.078
13
LNU73
25751.1
0.878
0.177
9


CONT.
—
10.300
—
0
LNU276
25433.2
0.839
0.705
6
LNU73
25754.2
0.878
0.393
9


LNU130
24913.6.
10.625
0.391
3
LNU279
25481.3
0.999
0.223
26
LNU9
25001.1
0.934
0.049
16


LNU136
14511.10.
11.375
0.002
10
LNU279
25484.3
0.995
0.011
26
LNU9
25001.7
0.899
0.313
12


LNU136
14513.6.
10.563
0.174
3
LNU279
25481.5
0.973
0.141
23
LNU9
25003.1
0.886
0.650
10


LNU136
14515.5.
10.500
0.759
2
LNU279
25481.2
0.953
0.575
20
LNU9
25001.3
0.858
0.278
7


LNU136
14515.1.
10.375
0.666
1
LNU279
25481.4
0.920
0.251
16
LNU9
25001.2
0.833
0.627
4


LNU142
27546.1.
10.875
0.193
6
LNU36
25562.3
1.007
0.297
27
CONT.
—
0.562
—
0


LNU142
27541.1.
10.500
0.264
2
LNU36
25562.7
0.933
0.623
18
LNU131
14005.5
0.869
0.001
55


LNU15
14123.13.
11.063
0.304
7
LNU36
25561.2
0.907
0.401
15
LNU131
14005.2
0.772
0.276
37


LNU15
14123.11.
10.917
0.474
6
LNU36
25562.4
0.829
0.518
5
LNU131
14002.15
0.714
0.004
27


LNU185
26474.2.
10.875
0.521
6
LNU53
25674.1
0.818
0.774
3
LNU131
14001.16
0.603
0.721
7


LNU185
26475.1.
10.750
0.424
4
LNU56
24694.1
0.987
0.484
25
LNU135
26204.2
0.930
0.182
66


LNU212
25833.2.
11.438
0.109
11
LNU56
24693.1
0.979
0.356
24
LNU135
26203.6
0.708
0.177
26


LNU212
25834.4.
11.250
0.358
9
LNU56
24691.2
0.926
0.180
17
LNU135
26203.3
0.686
0.434
22


LNU212
25834.5.
10.813
0.134
5
LNU56
24694.2
0.850
0.624
7
LNU135
26203.4
0.676
0.509
20


LNU212
25832.1.
10.500
0.573
2
LNU73
25755.1
1.109
0.000
40
LNU135
26203.1
0.647
0.276
15


LNU216
25984.1.
11.125
0.326
8
LNU73
25751.9
0.997
0.079
26
LNU161
14553.5
0.787
0.366
40


LNU216
25985.4.
10.688
0.056
4
LNU73
25751.1
0.985
0.011
25
LNU161
14552.7
0.603
0.548
7


LNU216
25984.6.
10.563
0.550
3
LNU73
25754.2
0.974
0.426
23
LNU173
25451.11
0.635
0.423
13


LNU228
26224.7.
11.000
0.456
7
LNU73
25751.8
0.965
0.469
22
LNU173
25451.5
0.585
0.523
4


LNU228
26222.4.
10.688
0.614
4
LNU9
25001.1
1.001
0.015
26
LNU181
25771.2
0.721
0.112
28


LNU228
26225.2.
10.625
0.632
3
LNU9
25001.7
0.979
0.222
24
LNU181
25774.1
0.651
0.165
16


LNU229
26111.7.
10.813
0.017
5
LNU9
25001.2
0.890
0.107
12
LNU181
25771.5
0.638
0.124
14


LNU229
26112.3.
10.750
0.270
4
LNU9
25001.3
0.821
0.598
4
LNU181
25771.6
0.637
0.042
13


LNU229
26112.6.
10.375
0.733
1
CONT.
—
0.609
—
0
LNU181
25771.11
0.597
0.228
6


LNU241
26234.1.
10.938
0.520
6
LNU131
14005.5
1.234
0.000
79
LNU181
25771.8
0.577
0.596
3


LNU253
26241.1.
10.938
0.005
6
LNU131
14005.2
0.916
0.334
33
LNU184
25394.3
0.813
0.180
45


LNU274
26261.3.
10.625
0.391
3
LNU131
14002.15
0.843
0.195
22
LNU184
25393.3
0.799
0.071
42


LNU274
26265.1.
10.563
0.768
3
LNU135
26204.2
1.253
0.247
82
LNU184
25394.1
0.755
0.192
34


LNU280
26162.1.
10.580
0.507
3
LNU135
26203.6
0.954
0.040
38
LNU184
25395.1
0.719
0.636
28


LNU280
26164.4.
10.479
0.461
2
LNU135
26203.4
0.904
0.479
31
LNU184
25393.1
0.640
0.376
14


LNU55
26015.1.
10.563
0.174
3
LNU135
26203.1
0.874
0.039
27
LNU184
25393.2
0.634
0.177
13


LNU81
26034.2.
10.750
0.270
4
LNU135
26203.3
0.790
0.705
15
LNU224
25871.3
0.797
0.041
42


LNU81
26031.2.
10.688
0.056
4
LNU161
14553.5
0.916
0.565
33
LNU224
25872.2
0.751
0.000
34


LNU81
26031.9.
10.563
0.656
3
LNU161
14553.6
0.776
0.049
12
LNU224
25874.1
0.726
0.011
29


CONT.
—
10.021
—
0
LNU173
25451.5
0.736
0.714
7
LNU224
25872.3
0.690
0.203
23


LNU119
26144.1.
10.500
0.098
5
LNU173
25451.2
0.718
0.488
4
LNU224
25874.4
0.662
0.314
18


LNU119
26142.5.
10.313
0.280
3
LNU181
25771.2
0.966
0.125
40
LNU246
25743.2
0.819
0.360
46


LNU130
24914.5.
10.688
0.037
7
LNU181
25771.11
0.876
0.000
27
LNU246
25744.4
0.760
0.459
35


LNU130
24913.6.
10.500
0.124
5
LNU181
25771.6
0.862
0.004
25
LNU246
25744.2
0.744
0.439
32


LNU130
24913.5.
10.313
0.280
3
LNU181
25774.1
0.763
0.105
11
LNU246
25743.1
0.712
0.087
27


LNU136
14511.10.
10.688
0.430
7
LNU181
25771.8
0.751
0.478
9
LNU246
25744.3
0.653
0.399
16


LNU136
14514.8.
10.500
0.124
5
LNU181
25771.5
0.731
0.334
6
LNU250
25592.2
0.752
0.213
34


LNU136
14515.1.
10.438
0.506
4
LNU184
25395.1
1.054
0.264
53
LNU250
25591.1
0.673
0.248
20


LNU136
14515.5.
10.438
0.506
4
LNU184
25394.3
1.053
0.371
53
LNU250
25592.1
0.640
0.589
14


LNU142
27546.2.
10.250
0.779
2
LNU184
25394.1
0.951
0.294
38
LNU250
25591.3
0.582
0.622
4


LNU142
27546.1.
10.188
0.607
2
LNU184
25393.3
0.929
0.024
35
LNU260
26404.8
0.683
0.025
22


LNU149
26174.7.
10.250
0.424
2
LNU184
25393.2
0.782
0.035
13
LNU260
26404.1
0.679
0.418
21


LNU15
14123.13.
10.875
0.015
9
LNU184
25393.1
0.753
0.732
9
LNU260
26403.1
0.656
0.142
17


LNU15
14124.12.
10.688
0.037
7
LNU224
25871.3
1.095
0.000
59
LNU260
26403.2
0.622
0.084
11


LNU15
14122.9.
10.313
0.628
3
LNU224
25874.1
1.000
0.000
45
LNU260
26404.7
0.619
0.672
10


LNU185
26474.1.
10.500
0.124
5
LNU224
25872.2
0.981
0.000
42
LNU276
25433.6
0.741
0.251
32


LNU185
26475.1.
10.125
0.677
1
LNU224
25872.3
0.841
0.025
22
LNU276
25431.1
0.651
0.008
16


LNU212
25834.4.
11.063
0.198
10
LNU224
25874.4
0.807
0.382
17
LNU276
25433.1
0.598
0.619
6


LNU212
25834.1.
10.750
0.027
7
LNU246
25743.1
1.000
0.316
45
LNU279
25481.5
0.724
0.000
29


LNU212
25834.5.
10.375
0.512
4
LNU246
25743.2
0.986
0.464
43
LNU279
25481.3
0.695
0.581
24


LNU212
25833.1.
10.188
0.607
2
LNU246
25744.2
0.977
0.436
42
LNU279
25484.3
0.694
0.583
24


LNU216
25982.2.
11.188
0.081
12
LNU246
25744.4
0.900
0.570
30
LNU279
25481.2
0.592
0.694
5


LNU216
25985.4.
11.000
0.009
10
LNU246
25744.3
0.766
0.516
11
LNU3
26122.2
0.726
0.545
29


LNU216
25982.1.
10.938
0.234
9
LNU250
25592.2
0.925
0.000
34
LNU3
26124.3
0.684
0.377
22


LNU216
25984.6.
10.313
0.746
3
LNU250
25591.1
0.792
0.492
15
LNU3
26123.5
0.643
0.491
15


LNU228
26225.2.
10.938
0.033
9
LNU250
25592.1
0.772
0.626
12
LNU3
26124.1
0.627
0.549
12


LNU228
26224.7.
10.771
0.553
7
LNU260
26404.7
0.835
0.567
21
LNU33
25553.3
0.772
0.058
37


LNU228
26222.4.
10.750
0.027
7
LNU260
26404.1
0.830
0.519
20
LNU33
25552.2
0.729
0.384
30


LNU228
26224.6.
10.375
0.672
4
LNU260
26404.8
0.822
0.210
19
LNU33
25553.2
0.703
0.091
25


LNU228
26222.1.
10.313
0.280
3
LNU276
25433.6
0.852
0.275
23
LNU33
25553.1
0.699
0.464
24


LNU229
26112.3.
10.938
0.394
9
LNU276
25433.5
0.811
0.162
17
LNU53
25674.6
0.675
0.202
20


LNU229
26111.5.
10.688
0.336
7
LNU276
25431.1
0.795
0.020
15
LNU53
25674.3
0.621
0.284
11


LNU229
26112.4.
10.625
0.175
6
LNU279
25481.3
0.943
0.510
37
LNU56
24694.2
0.667
0.187
19


LNU229
26111.7.
10.375
0.377
4
LNU279
25484.3
0.927
0.303
34
LNU56
24693.1
0.623
0.497
11


LNU241
26233.3.
10.313
0.697
3
LNU279
25481.5
0.884
0.004
28
LNU73
25751.8
0.621
0.689
11


LNU241
26232.4.
10.125
0.781
1
LNU279
25481.4
0.754
0.618
9
CONT.
—
0.920
—
0


LNU253
26242.1.
10.375
0.672
4
LNU3
26122.2
0.888
0.481
29
LNU130
24912.7.
0.993
0.491
8


LNU274
26265.1.
10.625
0.065
6
LNU33
25553.3
0.988
0.000
43
LNU130
24911.7.
0.932
0.736
1


LNU274
26261.3.
10.375
0.607
4
LNU33
25552.2
0.881
0.639
28
LNU130
24913.6.
0.928
0.690
1


LNU274
26264.2.
10.250
0.659
2
LNU33
25553.2
0.860
0.276
25
LNU136
14511.10.
1.024
0.000
11


LNU280
26164.4.
10.652
0.132
6
LNU33
25553.1
0.793
0.638
15
LNU136
14514.8.
0.976
0.214
6


LNU280
26162.1.
10.438
0.506
4
LNU53
25674.6
0.831
0.271
20
LNU142
27546.1.
0.931
0.592
1


LNU55
26013.4.
10.563
0.072
5
LNU56
24694.2
0.718
0.699
4
LNU149
26175.3.
0.950
0.244
3


LNU55
26013.3.
10.438
0.734
4
LNU73
25751.8
0.793
0.713
15
LNU15
14123.13.
0.985
0.389
7


LNU55
26013.9.
10.313
0.383
3
CONT.
—
0.998
—
0
LNU15
14123.11.
0.973
0.646
6


LNU81
26031.2.
10.813
0.019
8
LNU119
26144.2.
1.111
0.000
11
LNU15
14122.8.
0.927
0.724
1


LNU81
26031.10.
10.500
0.637
5
LNU130
24912.7.
1.170
0.471
17
LNU185
26475.1.
1.017
0.001
11







LNU130
24913.6.
1.124
0.000
13
LNU185
26474.1.
1.017
0.224
11







LNU130
24914.5.
1.091
0.507
9
LNU185
26474.2.
1.012
0.558
10







LNU130
24911.7.
1.065
0.479
7
LNU212
25834.4.
1.093
0.186
19







LNU136
14511.10.
1.152
0.000
15
LNU212
25834.5.
1.044
0.000
14







LNU136
14515.5.
1.131
0.504
13
LNU212
25833.2.
1.029
0.044
12







LNU142
27541.1.
1.162
0.162
16
LNU212
25834.1.
1.003
0.439
9







LNU149
26175.3.
1.088
0.262
9
LNU212
25832.1.
0.977
0.024
6







LNU15
14123.11.
1.074
0.619
8
LNU216
25982.1.
1.110
0.000
21







LNU15
14123.13.
1.060
0.270
6
LNU216
25984.1.
1.095
0.394
19







LNU185
26474.2.
1.181
0.474
18
LNU216
25984.6.
1.011
0.199
10







LNU185
26475.1.
1.164
0.276
17
LNU216
25985.4.
1.008
0.361
10







LNU185
26474.1.
1.075
0.648
8
LNU216
25982.2.
0.946
0.569
3







LNU212
25833.2.
1.253
0.141
26
LNU228
26222.4.
0.994
0.311
8







LNU212
25834.4.
1.144
0.191
15
LNU228
26224.7.
0.980
0.433
7







LNU212
25834.5.
1.137
0.293
14
LNU228
26224.6.
0.974
0.335
6







LNU212
25832.1.
1.024
0.771
3
LNU229
26112.3.
1.010
0.108
10







LNU216
25985.4.
1.190
0.281
19
LNU229
26111.7.
0.932
0.754
1







LNU216
25982.1.
1.189
0.144
19
LNU253
26241.1.
0.970
0.691
5







LNU216
25984.1.
1.159
0.328
16
LNU274
26262.2.
0.933
0.628
1







LNU216
25984.6.
1.047
0.057
5
LNU280
26162.1.
1.053
0.427
15







LNU228
26224.7.
1.169
0.003
17
LNU81
26031.9.
0.939
0.770
2







LNU228
26222.4.
1.089
0.204
9
LNU81
26034.2.
0.931
0.798
1







LNU229
26112.3.
1.154
0.000
16
CONT.
—
0.912
—
0







LNU253
26241.1.
1.110
0.200
11
LNU119
26142.5.
0.983
0.375
8







LNU253
26245.1.
1.050
0.357
5
LNU119
26141.1.
0.981
0.501
8







LNU274
26261.3.
1.028
0.543
3
LNU136
14514.8.
0.989
0.455
8







LNU277
25844.4.
1.059
0.506
6
LNU136
14511.10.
0.982
0.508
8







LNU280
26162.1.
1.139
0.546
14
LNU142
27546.2.
0.967
0.635
6







LNU55
26015.1.
1.133
0.325
14
LNU142
27545.1.
0.962
0.742
6







LNU81
26031.9.
1.080
0.744
8
LNU149
26174.7.
0.936
0.711
3







LNU8
26034.2.
1.063
0.673
6
LNU15
14122.8.
1.050
0.456
15







CONT.
—
0.973
—
0
LNU15
14123.13.
0.994
0.220
9







LNU119
26141.1.
1.245
0.391
28
LNU15
14122.9.
0.966
0.725
6







LNU119
26142.8.
1.071
0.613
10
LNU15
14124.12.
0.932
0.764
2







LNU119
26142.5.
1.063
0.417
9
LNU185
26474.1.
1.126
0.129
23







LNU130
24913.5.
1.121
0.205
15
LNU185
26473.1.
0.976
0.332
7







LNU130
24914.5.
1.060
0.457
9
LNU212
25834.4.
1.075
0.053
18







LNU130
24912.7.
1.044
0.766
7
LNU212
25834.1.
0.952
0.524
4







LNU130
24913.6.
1.041
0.655
7
LNU212
25833.2.
0.940
0.733
3







LNU136
14515.1.
1.065
0.451
9
LNU216
25985.4.
1.081
0.242
19







LNU136
14514.8.
1.048
0.675
8
LNU216
25982.2.
1.023
0.157
12







LNU136
14511.10.
1.040
0.682
7
LNU216
25982.1.
1.010
0.295
11







LNU142
27546.1.
1.048
0.483
8
LNU216
25984.6.
0.974
0.511
7







LNU142
27546.2.
1.048
0.622
8
LNU228
26225.2.
1.054
0.065
16







LNU149
26174.7.
1.017
0.729
5
LNU228
26222.4.
1.004
0.233
10







LNU15
14124.12.
1.132
0.340
16
LNU228
26222.1.
0.978
0.304
7







LNU15
14122.8.
1.119
0.482
15
LNU228
26224.6.
0.951
0.727
4







LNU15
14123.11.
1.082
0.717
11
LNU229
26111.5.
1.050
0.063
15







LNU15
14123.13.
1.052
0.469
8
LNU229
26112.3.
1.039
0.668
14







LNU185
26474.1.
1.258
0.042
29
LNU229
26111.7.
1.035
0.371
13







LNU185
26473.1.
1.004
0.773
3
LNU229
26112.4.
0.971
0.420
6







LNU212
25834.4.
1.176
0.138
21
LNU253
26242.1.
0.986
0.260
8







LNU212
25834.5.
1.099
0.265
13
LNU253
26241.1.
0.953
0.786
4







LNU212
25833.2.
1.063
0.408
9
LNU274
26262.2.
0.967
0.439
6







LNU216
25985.4.
1.248
0.201
28
LNU280
26162.1.
1.023
0.127
12







LNU216
25984.6.
1.077
0.653
11
LNU55
26013.4.
0.969
0.639
6







LNU216
25982.1.
1.068
0.386
10
LNU55
26013.9.
0.936
0.738
3







LNU216
25984.4.
1.025
0.757
5
LNU81
26031.2.
1.025
0.455
12







LNU228
26225.2.
1.254
0.054
29
LNU81
26031.10.
0.974
0.759
7







LNU228
26222.1.
1.154
0.136
19












LNU228
26224.7.
1.153
0.423
19












LNU228
26222.4.
1.135
0.165
17












LNU228
26224.6.
1.123
0.227
15












LNU229
26112.4.
1.185
0.331
22












LNU229
26111.7.
1.176
0.394
21












LNU229
26111.5.
1.160
0.295
19












LNU229
26112.3.
1.129
0.305
16












LNU241
26233.3.
1.137
0.596
17












LNU241
26234.1.
1.068
0.385
10












LNU241
26232.4.
1.065
0.399
9












LNU253
26242.1.
1.053
0.632
8












LNU253
26241.1.
1.045
0.505
7












LNU274
26265.1.
1.098
0.634
13












LNU274
26262.1.
1.056
0.558
8












LNU280
26162.1.
1.190
0.211
22












LNU55
26015.1.
1.104
0.272
13












LNU55
26013.4.
1.084
0.388
11












LNU81
26031.10.
1.169
0.541
20












LNU81
26031.2.
1.089
0.427
12





“CONT.”—Control; “Ave.”—Average; “% Incr.” = % increment.






The genes listed in Table 81 improved plant NUE when grown at standard nitrogen concentration levels. These genes produced faster developing plants when grown under limiting nitrogen growth conditions, compared to control plants as measured by growth rate of rosette area, rosette diameter and plot coverage. The genes were cloned under the regulation of a constitutive (At6669) and root preferred promoter (RootP). The evaluation of each gene was performed by testing the performance of different number of events. Event with p-value <0.1 was considered statistically significant.







TABLE 81







Genes showing improved rosette growth performance at limiting nitrogen growth conditions













RGR Of Rosette

RGR Of Rosette

RGR Of Plot



Area

Diameter

Coverage





















Gene


P-
%
Gene


P-
%
Gene


P-
%


Name
Event #
Ave.
Value
incr.
Name
Event #
Ave.
Value
incr.
Name
Event #
Ave.
Value
incr.
























CONT.
—
0.488
—
0
CONT.
—
0.279
—
0
CONT.
—
3.86
—
0


LNU100
14472.2
0.673
0.007
38
LNU100
14472.2
0.341
0.009
22
LNU100
14472.2
5.39
0.008
39


LNU100
14471.4
0.573
0.204
17
LNU100
14471.4
0.314
0.127
13
LNU100
14471.4
4.59
0.190
19


LNU104
25033.3
0.708
0.002
45
LNU100
14474.3
0.294
0.500
5
LNU100
14474.3
4.01
0.785
4


LNU104
25032.2
0.654
0.019
34
LNU100
14473.3
0.293
0.539
5
LNU104
25033.3
5.66
0.002
47


LNU104
25032.1
0.590
0.122
21
LNU104
25033.3
0.359
0.001
29
LNU104
25032.2
5.24
0.018
36


LNU104
25033.1
0.539
0.432
10
LNU104
25032.1
0.321
0.078
15
LNU104
25032.1
4.72
0.115
22


LNU106
14481.1
0.646
0.025
32
LNU104
25032.2
0.315
0.115
13
LNU104
25033.1
4.31
0.400
12


LNU106
14483.5
0.591
0.140
21
LNU104
25033.1
0.299
0.369
7
LNU106
14481.1
5.17
0.024
34


LNU106
14483.2
0.555
0.315
14
LNU106
14481.1
0.341
0.015
22
LNU106
14483.5
4.73
0.132
22


LNU106
14484.3
0.543
0.412
11
LNU106
14483.5
0.331
0.042
19
LNU106
14483.2
4.44
0.292
15


LNU114
25041.2
0.534
0.497
9
LNU106
14483.2
0.305
0.245
9
LNU106
14484.3
4.34
0.381
13


LNU114
25042.1
0.528
0.545
8
LNU106
14484.3
0.296
0.446
6
LNU114
25041.2
4.27
0.461
11


LNU155
14525.1
0.573
0.193
17
LNU114
25041.2
0.299
0.401
7
LNU155
14525.1
4.58
0.181
19


LNU155
14523.5
0.542
0.422
11
LNU114
25041.1
0.295
0.506
6
LNU155
14523.5
4.34
0.391
12


LNU213
24654.4
0.558
0.285
14
LNU114
25042.1
0.289
0.660
4
LNU213
24654.4
4.46
0.265
16


LNU218
24781.7
0.637
0.029
31
LNU213
24653.2
0.307
0.264
10
LNU218
24781.7
5.10
0.029
32


LNU218
24781.4
0.628
0.046
29
LNU213
24652.4
0.295
0.527
6
LNU218
24781.4
5.02
0.045
30


LNU218
24784.2
0.586
0.152
20
LNU218
24781.7
0.353
0.002
27
LNU218
24784.2
4.68
0.143
21


LNU23
25163.5
0.601
0.157
23
LNU218
24781.4
0.328
0.045
18
LNU23
25163.5
4.81
0.147
25


LNU23
25163.6
0.537
0.492
10
LNU218
24784.2
0.314
0.139
13
LNU23
25163.6
4.29
0.457
11


LNU28
25171.2
0.588
0.137
20
LNU218
24781.2
0.286
0.762
2
LNU28
25171.2
4.70
0.129
22


LNU28
25171.1
0.583
0.157
19
LNU23
25163.5
0.341
0.050
22
LNU28
25171.1
4.66
0.148
21


LNU28
25174.5
0.520
0.677
7
LNU23
25163.6
0.309
0.239
11
LNU28
25174.5
4.16
0.633
8


LNU4
25133.3
0.591
0.127
21
LNU28
25171.1
0.349
0.003
25
LNU4
25133.3
4.73
0.120
22


LNU4
25134.1
0.515
0.691
5
LNU28
25171.2
0.327
0.034
17
LNU4
25134.1
4.12
0.641
7


LNU40
24794.3
0.619
0.053
27
LNU28
25174.5
0.305
0.374
9
LNU40
24794.3
4.95
0.051
28


LNU40
24794.4
0.585
0.153
20
LNU28
25171.4
0.289
0.676
4
LNU40
24794.4
4.41
0.346
14


LNU46
14462.5
0.751
0.001
54
LNU4
25133.3
0.317
0.088
14
LNU46
14462.5
6.01
0.001
56


LNU46
14464.4
0.712
0.008
46
LNU4
25134.1
0.291
0.636
4
LNU46
14464.4
5.69
0.008
47


LNU46
14464.1
0.540
0.430
11
LNU4
25131.1
0.288
0.705
3
LNU46
14464.1
4.32
0.398
12


LNU46
14462.1
0.532
0.511
9
LNU40
24794.4
0.326
0.045
17
LNU46
14462.1
4.26
0.473
10


LNU46
14463.1
0.532
0.506
9
LNU40
24794.3
0.304
0.256
9
LNU46
14463.1
4.25
0.468
10


LNU48
24801.4
0.634
0.032
30
LNU46
14464.4
0.356
0.010
28
LNU48
24801.4
5.07
0.032
31


LNU48
24802.1
0.557
0.289
14
LNU46
14462.5
0.338
0.020
21
LNU48
24802.1
4.46
0.268
15


LNU48
24804.4
0.512
0.714
5
LNU46
14464.1
0.304
0.264
9
LNU48
24804.4
4.09
0.661
6


LNU63
24814.2
0.572
0.210
17
LNU46
14462.1
0.298
0.416
7
LNU63
24814.2
4.58
0.196
19


LNU63
24814.3
0.529
0.538
8
LNU46
14463.1
0.290
0.614
4
LNU63
24814.3
4.23
0.498
10


LNU63
24811.2
0.511
0.723
5
LNU48
24801.4
0.335
0.015
20
LNU63
24811.2
4.09
0.670
6


LNU7
25081.1
0.570
0.217
17
LNU48
24802.1
0.312
0.132
12
LNU7
25081.1
4.56
0.203
18


LNU7
25082.2
0.540
0.431
11
LNU63
24814.3
0.307
0.219
10
LNU7
25082.2
4.32
0.399
12


LNU8
25063.6
0.531
0.508
9
LNU63
24814.2
0.300
0.368
7
LNU8
25063.6
4.25
0.469
10


LNU8
25062.1
0.530
0.516
9
LNU63
24811.2
0.289
0.636
4
LNU8
25062.1
4.24
0.478
10


LNU8
25061.2
0.512
0.712
5
LNU7
25081.1
0.315
0.109
13
LNU8
25061.2
4.10
0.660
6


LNU8
25062.2
0.511
0.734
5
LNU7
25082.2
0.312
0.187
12
LNU8
25062.2
4.09
0.681
6


LNU94
24833.3
0.560
0.273
15
LNU8
25061.2
0.294
0.504
5
LNU94
24833.3
4.19
0.543
9


LNU96
25071.2
0.621
0.062
27
LNU8
25063.6
0.287
0.699
3
LNU96
25071.2
4.96
0.060
29


LNU96
25073.4
0.524
0.612
7
LNU94
24833.3
0.298
0.382
7
LNU96
25073.4
4.19
0.569
9


LNU96
25071.3
0.510
0.738
4
LNU96
25071.2
0.321
0.091
15
CONT.
—
5.49
—
0


CONT.
—
0.686
—
0
LNU96
25073.4
0.301
0.419
8
LNU113
25631.7
6.03
0.397
10


LNU113
25631.7
0.754
0.397
10
LNU96
25071.3
0.294
0.527
5
LNU113
25631.3
5.87
0.555
7


LNU113
25631.3
0.733
0.555
7
LNU96
25074.1
0.292
0.559
5
LNU113
25631.1
5.75
0.676
5


LNU113
25631.1
0.718
0.676
5
CONT.
—
0.328
—
0
LNU140
14115.1
5.68
0.766
3


LNU140
14115.1
0.710
0.766
3
LNU113
25631.3
0.376
0.086
15
LNU148
25685.6
7.18
0.010
31


LNU148
25685.6
0.898
0.010
31
LNU113
25631.7
0.355
0.277
8
LNU148
25685.1
6.24
0.232
14


LNU148
25685.1
0.779
0.232
14
LNU113
25631.1
0.347
0.436
6
LNU148
25685.9
5.67
0.772
3


LNU148
25685.9
0.709
0.772
3
LNU120
25463.3
0.335
0.779
2
LNU5
14043.7
6.18
0.270
13


LNU5
14043.7
0.773
0.270
13
LNU132
14102.6
0.348
0.435
6
LNU74
25444.1
6.11
0.317
11


LNU74
25444.1
0.763
0.317
11
LNU148
25685.6
0.366
0.136
12
LNU98
25763.2
5.77
0.655
5


LNU98
25763.2
0.721
0.655
5
LNU148
25685.1
0.357
0.244
9
CONT.
—
5.16
—
0


CONT.
—
0.653
—
0
LNU148
25685.9
0.338
0.668
3
LNU113
25631.9
5.82
0.422
13


LNU113
25631.9
0.727
0.470
11
LNU287
24674.6
0.336
0.749
3
LNU148
25685.1
5.80
0.439
12


LNU148
25685.1
0.725
0.488
11
LNU5
14043.7
0.358
0.226
9
LNU72
24963.7
5.72
0.507
11


LNU72
24963.7
0.715
0.559
9
LNU68
14035.5
0.335
0.761
2
LNU72
24962.3
5.56
0.633
8


LNU72
24962.3
0.695
0.690
6
LNU74
25444.1
0.348
0.418
6
LNU98
25763.2
5.81
0.437
12


LNU98
25763.2
0.726
0.486
11
LNU74
25441.2
0.343
0.592
5
CONT.
—
4.13
—
0


CONT.
—
0.534
—
0
LNU74
25443.3
0.340
0.633
4
LNU117
25931.4
7.72
0.000
87


LNU117
25931.4
0.965
0.000
81
LNU74
25443.2
0.336
0.746
2
LNU117
25931.2
6.79
0.005
64


LNU117
25931.2
0.849
0.002
59
LNU87
24714.3
0.335
0.771
2
LNU117
25933.3
6.49
0.006
57


LNU117
25933.3
0.811
0.001
52
CONT.
—
0.333
—
0
LNU117
25931.1
6.13
0.040
49


LNU117
25931.1
0.767
0.027
44
LNU113
25631.9
0.355
0.607
7
LNU117
25932.4
5.82
0.116
41


LNU117
25932.4
0.728
0.107
36
LNU148
25685.1
0.351
0.669
6
LNU122
25333.2
7.24
0.002
75


LNU122
25333.2
0.905
0.000
69
LNU74
25443.5
0.344
0.792
3
LNU122
25332.1
6.68
0.017
62


LNU122
25332.1
0.835
0.011
56
LNU98
25763.2
0.353
0.647
6
LNU122
25332.2
6.18
0.015
50


LNU122
25332.2
0.773
0.005
45
CONT.
—
0.241
—
0
LNU122
25333.1
5.72
0.064
38


LNU122
25333.1
0.715
0.041
34
LNU117
25931.4
0.364
0.004
51
LNU122
25332.5
5.66
0.056
37


LNU122
25332.5
0.708
0.029
32
LNU117
25931.2
0.357
0.003
48
LNU125
25941.4
7.61
0.001
84


LNU125
25941.4
0.951
0.000
78
LNU117
25931.1
0.335
0.026
39
LNU125
25943.3
6.79
0.003
64


LNU125
25943.3
0.849
0.001
59
LNU117
25933.3
0.332
0.026
38
LNU125
25943.2
6.47
0.043
57


LNU125
25943.2
0.809
0.036
51
LNU117
25932.4
0.331
0.098
37
LNU125
25941.2
6.03
0.055
46


LNU125
25941.2
0.754
0.041
41
LNU122
25333.2
0.339
0.011
41
LNU125
25944.3
5.66
0.113
37


LNU125
25944.3
0.707
0.096
32
LNU122
25332.1
0.316
0.078
31
LNU138
14074.5
7.47
0.001
81


LNU138
14074.5
0.933
0.000
75
LNU122
25332.2
0.316
0.047
31
LNU138
14074.6
7.05
0.010
71


LNU138
14074.6
0.882
0.006
65
LNU122
25333.1
0.313
0.055
30
LNU138
14071.5
6.56
0.009
59


LNU138
14071.5
0.820
0.003
54
LNU122
25332.5
0.299
0.138
24
LNU138
14072.8
4.88
0.351
18


LNU138
14072.8
0.610
0.348
14
LNU125
25941.4
0.359
0.006
49
LNU138
14072.5
4.76
0.424
15


LNU138
14072.5
0.595
0.436
11
LNU125
25943.2
0.340
0.057
41
LNU180
24721.2
8.62
0.000
109


LNU180
24721.2
1.077
0.000
102
LNU125
25943.3
0.339
0.013
41
LNU180
24723.1
8.22
0.000
99


LNU180
24723.1
1.028
0.000
92
LNU125
25944.3
0.322
0.043
34
LNU180
24721.4
8.02
0.000
94


LNU180
24721.4
1.002
0.000
88
LNU125
25941.2
0.321
0.079
33
LNU180
24722.2
7.40
0.005
79


LNU180
24722.2
0.925
0.003
73
LNU138
14074.5
0.375
0.001
55
LNU180
24724.1
7.06
0.008
71


LNU180
24724.1
0.883
0.004
65
LNU138
14074.6
0.360
0.011
49
LNU220
25405.2
7.25
0.002
76


LNU220
25405.2
0.906
0.001
70
LNU138
14071.5
0.355
0.003
47
LNU220
25405.5
7.21
0.003
75


LNU220
25405.5
0.901
0.001
69
LNU138
14072.5
0.312
0.045
30
LNU220
25405.1
7.03
0.006
70


LNU220
25405.1
0.879
0.003
64
LNU138
14072.8
0.299
0.100
24
LNU220
25405.6
5.97
0.036
45


LNU220
25405.6
0.746
0.020
40
LNU180
24721.2
0.404
0.000
67
LNU220
25405.3
5.49
0.133
33


LNU220
25405.3
0.686
0.111
28
LNU180
24721.4
0.397
0.001
65
LNU230
25413.1
7.87
0.001
90


LNU230
25413.1
0.983
0.000
84
LNU180
24723.1
0.393
0.000
63
LNU230
25412.1
7.21
0.003
75


LNU230
25412.1
0.902
0.001
69
LNU180
24722.2
0.372
0.007
54
LNU230
25415.1
6.63
0.013
60


LNU230
25415.1
0.828
0.007
55
LNU180
24724.1
0.355
0.026
47
LNU230
25413.2
6.35
0.031
54


LNU230
25412.2
0.807
0.005
51
LNU220
25405.5
0.369
0.003
53
LNU230
25412.2
6.05
0.032
46


LNU230
25413.2
0.793
0.021
48
LNU220
25405.2
0.353
0.009
47
LNU234
25014.8
6.95
0.002
68


LNU234
25014.8
0.868
0.001
63
LNU220
25405.6
0.340
0.010
41
LNU234
25014.1
6.22
0.021
51


LNU234
25014.1
0.778
0.010
46
LNU220
25405.1
0.332
0.043
38
LNU234
25014.6
6.08
0.019
47


LNU234
25014.6
0.759
0.007
42
LNU220
25405.3
0.320
0.039
33
LNU234
25014.4
5.76
0.047
40


LNU234
25014.4
0.720
0.024
35
LNU230
25413.1
0.382
0.003
59
LNU234
25014.5
5.67
0.121
37


LNU234
25014.5
0.709
0.107
33
LNU230
25412.1
0.363
0.004
51
LNU25
14082.8
7.38
0.002
79


LNU25
14082.8
0.923
0.001
73
LNU230
25412.2
0.331
0.024
37
LNU25
14083.7
7.21
0.001
75


LNU25
14083.7
0.901
0.000
69
LNU230
25415.1
0.323
0.091
34
LNU25
14082.9
7.12
0.001
72


LNU25
14082.9
0.890
0.000
67
LNU230
25413.2
0.317
0.083
32
LNU25
14084.6
7.08
0.008
71


LNU25
14084.6
0.885
0.005
66
LNU234
25014.8
0.370
0.004
54
LNU25
14083.1
6.98
0.002
69


LNU25
14083.1
0.873
0.000
63
LNU234
25014.1
0.339
0.026
41
LNU254
25782.4
7.44
0.001
80


LNU254
25782.4
0.931
0.000
74
LNU234
25014.5
0.336
0.038
39
LNU254
25781.3
7.41
0.001
79


LNU254
25781.3
0.927
0.000
73
LNU234
25014.6
0.325
0.019
35
LNU254
25781.5
6.76
0.005
64


LNU254
25781.5
0.845
0.002
58
LNU234
25014.4
0.323
0.035
34
LNU254
25783.1
6.35
0.026
54


LNU254
25783.1
0.793
0.017
48
LNU25
14082.9
0.381
0.001
58
LNU254
25782.5
6.16
0.018
49


LNU254
25782.5
0.769
0.007
44
LNU25
14082.8
0.367
0.013
52
LNU263
25794.3
7.49
0.001
81


LNU263
25794.3
0.937
0.000
75
LNU25
14083.1
0.361
0.002
50
LNU263
25794.8
7.41
0.004
79


LNU263
25794.8
0.926
0.003
73
LNU25
14084.6
0.356
0.038
47
LNU263
25791.3
7.30
0.001
77


LNU263
25791.3
0.912
0.000
71
LNU25
14083.7
0.343
0.014
42
LNU263
25794.6
7.09
0.002
72


LNU263
25794.6
0.886
0.001
66
LNU254
25781.3
0.376
0.002
56
LNU263
25792.2
6.66
0.006
61


LNU263
25792.2
0.833
0.002
56
LNU254
25782.4
0.363
0.007
50
LNU267
25804.3
6.91
0.006
67


LNU267
25804.3
0.864
0.003
62
LNU254
25781.5
0.356
0.003
48
LNU267
25804.4
6.88
0.002
67


LNU267
25804.4
0.860
0.000
61
LNU254
25782.5
0.345
0.007
43
LNU267
25801.1
6.29
0.022
52


LNU267
25801.1
0.786
0.012
47
LNU254
25783.1
0.335
0.028
39
LNU267
25803.1
6.23
0.013
51


LNU267
25803.1
0.778
0.004
46
LNU263
25794.3
0.383
0.000
59
LNU267
25802.1
5.92
0.039
43


LNU267
25802.1
0.740
0.022
39
LNU263
25794.6
0.374
0.002
55
LNU271
25911.4
8.12
0.000
97


LNU271
25911.4
1.015
0.000
90
LNU263
25794.8
0.370
0.007
53
LNU271
25912.1
6.51
0.019
58


LNU271
25912.1
0.862
0.001
61
LNU263
25791.3
0.354
0.007
47
LNU271
25913.3
6.28
0.014
52


LNU271
25913.3
0.785
0.005
47
LNU263
25792.2
0.350
0.010
45
LNU271
25912.2
5.66
0.057
37


LNU271
25912.2
0.707
0.030
32
LNU267
25804.3
0.367
0.006
52
LNU271
25913.2
4.79
0.488
16


LNU271
25913.2
0.599
0.531
12
LNU267
25803.1
0.357
0.003
48
LNU278
25812.3
7.83
0.000
89


LNU278
25812.3
0.978
0.000
83
LNU267
25802.1
0.346
0.008
43
LNU278
25814.3
7.58
0.000
84


LNU278
25814.3
0.948
0.000
77
LNU267
25804.4
0.335
0.010
39
LNU278
25812.2
6.21
0.018
50


LNU278
25812.2
0.776
0.008
45
LNU267
25801.1
0.319
0.053
32
LNU278
25814.1
5.82
0.051
41


LNU278
25814.1
0.727
0.030
36
LNU271
25911.4
0.399
0.000
65
LNU278
25813.2
5.19
0.173
26


LNU278
25813.2
0.648
0.132
21
LNU271
25913.3
0.359
0.004
49
LNU36
25562.9
7.33
0.000
78


LNU36
25562.9
0.917
0.000
72
LNU271
25912.2
0.332
0.013
38
LNU36
25562.3
6.37
0.011
54


LNU36
25562.3
0.797
0.004
49
LNU271
25912.1
0.317
0.056
31
LNU36
25562.4
5.88
0.031
42


LNU36
25562.4
0.735
0.013
38
LNU271
25913.2
0.265
0.611
10
LNU36
25562.7
5.45
0.090
32


LNU36
25562.7
0.681
0.053
27
LNU278
25812.3
0.398
0.000
65
LNU36
25561.2
5.42
0.105
31


LNU36
25561.2
0.678
0.069
27
LNU278
25814.3
0.352
0.006
46
LNU43
14422.8
6.93
0.002
68


LNU43
14422.8
0.867
0.000
62
LNU278
25812.2
0.330
0.019
37
LNU43
14422.9
6.46
0.007
56


LNU43
14422.9
0.808
0.002
51
LNU278
25814.1
0.290
0.263
20
LNU43
14421.1
6.45
0.008
56


LNU43
14421.1
0.806
0.002
51
LNU278
25813.2
0.282
0.286
17
LNU43
14423.6
5.85
0.033
42


LNU43
14423.6
0.731
0.014
37
LNU36
25562.9
0.342
0.013
42
LNU43
14423.7
5.70
0.082
38


LNU43
14423.7
0.712
0.060
33
LNU36
25561.2
0.326
0.021
35
LNU45
25052.12
7.16
0.003
73


LNU45
25052.12
0.896
0.001
68
LNU36
25562.3
0.324
0.025
34
LNU45
25052.8
6.58
0.004
59


LNU45
25052.8
0.823
0.001
54
LNU36
25562.4
0.300
0.110
24
LNU45
25052.9
6.10
0.017
48


LNU45
25052.9
0.762
0.006
43
LNU36
25562.7
0.288
0.196
19
LNU45
25053.4
5.57
0.185
35


LNU45
25053.4
0.697
0.182
30
LNU43
14422.8
0.347
0.006
44
LNU45
25052.11
5.20
0.176
26


LNU45
25052.11
0.650
0.138
22
LNU43
14422.9
0.336
0.013
39
LNU67
25821.5
5.92
0.062
43


LNU67
25821.5
0.740
0.046
39
LNU43
14421.1
0.335
0.014
39
LNU67
25824.5
5.91
0.067
43


LNU67
25824.5
0.739
0.051
38
LNU43
14423.6
0.325
0.027
35
LNU67
25823.5
5.42
0.149
31


LNU67
25823.5
0.678
0.127
27
LNU43
14423.7
0.306
0.158
27
LNU67
25824.3
5.29
0.208
28


LNU67
25824.3
0.661
0.194
24
LNU45
25052.12
0.365
0.006
51
LNU67
25821.4
4.98
0.267
21


LNU67
25821.4
0.623
0.236
17
LNU45
25052.8
0.303
0.099
26
CONT.
—
3.80
—
0


CONT.
—
0.475
—
0
LNU45
25052.11
0.302
0.097
25
LNU100
14474.2
5.66
0.002
49


LNU100
14474.2
0.708
0.002
49
LNU45
25052.9
0.295
0.152
22
LNU100
14472.1
5.36
0.011
41


LNU100
14472.1
0.670
0.011
41
LNU45
25053.4
0.266
0.626
10
LNU100
14473.1
5.14
0.018
35


LNU100
14473.1
0.642
0.018
35
LNU67
25824.5
0.320
0.090
33
LNU100
14473.3
5.11
0.031
34


LNU100
14473.3
0.638
0.031
34
LNU67
25824.3
0.316
0.058
31
LNU100
14471.4
4.46
0.241
17


LNU100
14471.4
0.557
0.241
17
LNU67
25821.5
0.307
0.124
27
LNU104
25033.1
4.65
0.121
23


LNU104
25033.1
0.582
0.121
23
LNU67
25823.5
0.284
0.341
18
LNU104
25032.2
4.64
0.146
22


LNU104
25032.2
0.580
0.146
22
LNU67
25821.4
0.252
0.790
4
LNU104
25032.1
4.40
0.263
16


LNU104
25032.1
0.550
0.263
16
CONT.
—
0.289
—
0
LNU104
25033.3
4.39
0.253
16


LNU104
25033.3
0.549
0.253
16
LNU100
14474.2
0.374
0.011
29
LNU104
25033.8
4.02
0.705
6


LNU104
25033.8
0.502
0.705
6
LNU100
14473.3
0.359
0.039
24
LNU106
14483.2
5.70
0.001
50


LNU106
14483.2
0.713
0.001
50
LNU100
14472.1
0.356
0.060
23
LNU106
14481.1
4.90
0.066
29


LNU106
14481.1
0.612
0.066
29
LNU100
14473.1
0.351
0.053
22
LNU106
14483.5
4.59
0.134
21


LNU106
14483.5
0.573
0.134
21
LNU100
14471.4
0.335
0.163
16
LNU106
14484.3
4.41
0.281
16


LNU106
14484.3
0.551
0.281
16
LNU104
25033.3
0.334
0.157
15
LNU114
25044.11
5.15
0.023
36


LNU114
25044.11
0.643
0.023
36
LNU104
25033.1
0.330
0.247
14
LNU114
25041.2
4.91
0.044
29


LNU114
25041.2
0.614
0.044
29
LNU104
25032.1
0.324
0.262
12
LNU114
25042.1
4.54
0.175
19


LNU114
25042.1
0.567
0.175
19
LNU104
25033.8
0.315
0.468
9
LNU114
25041.1
4.37
0.379
15


LNU114
25041.1
0.546
0.379
15
LNU104
25032.2
0.312
0.475
8
LNU114
25044.4
4.13
0.578
9


LNU114
25044.4
0.516
0.578
9
LNU106
14483.2
0.397
0.001
38
LNU117
25932.4
5.18
0.013
36


LNU117
25932.4
0.647
0.013
36
LNU106
14481.1
0.345
0.110
19
LNU117
25931.4
4.89
0.043
29


LNU117
25931.4
0.612
0.043
29
LNU106
14483.5
0.326
0.241
13
LNU117
25931.1
4.26
0.438
12


LNU117
25931.1
0.532
0.438
12
LNU106
14484.3
0.317
0.401
10
LNU117
25931.2
4.20
0.448
11


LNU117
25931.2
0.525
0.448
11
LNU114
25044.11
0.348
0.075
20
LNU155
14523.5
4.92
0.050
30


LNU155
14523.5
0.616
0.050
30
LNU114
25041.2
0.347
0.066
20
LNU155
14524.8
4.12
0.540
9


LNU155
14524.8
0.515
0.540
9
LNU114
25041.1
0.338
0.204
17
LNU180
24723.1
4.93
0.037
30


LNU180
24723.1
0.617
0.037
30
LNU114
25042.1
0.335
0.153
16
LNU180
24722.2
4.47
0.282
18


LNU180
24722.2
0.559
0.282
18
LNU117
25932.4
0.374
0.011
30
LNU218
24781.4
5.34
0.007
41


LNU218
24781.4
0.668
0.007
41
LNU117
25931.4
0.326
0.236
13
LNU218
24781.1
4.83
0.066
27


LNU218
24781.1
0.604
0.066
27
LNU117
25931.2
0.316
0.405
9
LNU218
24781.6
4.00
0.696
5


LNU218
24781.6
0.501
0.696
5
LNU117
25931.1
0.314
0.458
9
LNU218
24781.2
3.96
0.758
4


LNU218
24781.2
0.495
0.758
4
LNU155
14523.5
0.329
0.228
14
LNU254
25782.5
4.78
0.073
26


LNU254
25782.5
0.598
0.073
26
LNU155
14524.8
0.320
0.324
11
LNU4
25134.3
5.40
0.009
42


LNU4
25134.3
0.675
0.009
42
LNU180
24722.2
0.354
0.102
23
LNU4
25134.2
4.65
0.115
23


LNU4
25134.2
0.581
0.115
23
LNU180
24723.1
0.349
0.060
21
LNU4
25131.1
4.56
0.170
20


LNU4
25131.1
0.570
0.170
20
LNU180
24721.2
0.304
0.629
5
LNU4
25133.3
4.15
0.504
9


LNU4
25133.3
0.519
0.504
9
LNU218
24781.4
0.386
0.004
34
LNU40
24794.3
4.55
0.220
20


LNU40
24794.3
0.569
0.220
20
LNU218
24781.1
0.365
0.020
27
LNU40
24792.1
4.42
0.230
17


LNU40
24792.1
0.553
0.230
17
LNU218
24781.2
0.329
0.201
14
LNU40
24794.4
4.00
0.696
5


LNU40
24794.4
0.500
0.696
5
LNU218
24781.6
0.314
0.430
9
LNU46
14462.5
5.13
0.024
35


LNU46
14462.5
0.642
0.024
35
LNU254
25782.5
0.353
0.046
22
LNU46
14462.1
4.18
0.458
10


LNU46
14462.1
0.523
0.458
10
LNU4
25134.3
0.368
0.029
28
LNU46
14464.4
4.16
0.488
10


LNU46
14464.4
0.520
0.488
10
LNU4
25134.2
0.324
0.272
12
LNU48
24801.4
4.69
0.098
24


LNU48
24801.4
0.586
0.098
24
LNU4
25131.1
0.324
0.294
12
LNU48
24803.2
4.49
0.231
18


LNU48
24803.2
0.562
0.231
18
LNU4
25133.3
0.314
0.437
9
LNU63
24812.3
5.17
0.015
36


LNU48
24802.2
0.503
0.687
6
LNU40
24794.3
0.336
0.199
16
LNU63
24814.7
4.35
0.320
14


LNU63
24812.3
0.646
0.015
36
LNU40
24792.1
0.311
0.470
8
LNU7
25082.2
4.87
0.070
28


LNU63
24814.7
0.543
0.320
14
LNU40
24794.4
0.310
0.496
7
LNU7
25083.1
4.37
0.289
15


LNU7
25082.2
0.609
0.070
28
LNU46
14462.5
0.335
0.190
16
LNU7
25083.3
4.21
0.443
11


LNU7
25083.1
0.546
0.289
15
LNU46
14462.1
0.323
0.265
12
LNU8
25063.6
5.22
0.013
37


LNU7
25083.3
0.526
0.443
11
LNU46
14464.4
0.312
0.454
8
LNU8
25061.2
4.50
0.179
19


LNU8
25063.6
0.652
0.013
37
LNU48
24803.2
0.337
0.175
17
LNU94
24833.3
4.45
0.240
17


LNU8
25061.2
0.563
0.179
19
LNU48
24801.4
0.334
0.166
16
CONT.
—
5.26
—
0


LNU94
24833.3
0.556
0.240
17
LNU48
24802.2
0.310
0.515
7
LNU122
25332.1
6.26
0.154
19


CONT.
—
0.658
—
0
LNU48
24804.4
0.300
0.715
4
LNU122
25332.2
6.17
0.190
17


LNU122
25332.1
0.782
0.154
19
LNU63
24812.3
0.356
0.041
23
LNU122
25332.5
5.78
0.455
10


LNU122
25332.2
0.771
0.190
17
LNU63
24814.7
0.327
0.239
13
LNU125
25943.2
5.69
0.530
8


LNU122
25332.5
0.723
0.455
10
LNU63
24814.2
0.310
0.494
7
LNU125
25941.4
5.58
0.643
6


LNU125
25943.2
0.711
0.530
8
LNU7
25082.2
0.341
0.128
18
LNU125
25944.1
5.44
0.797
3


LNU125
25941.4
0.697
0.643
6
LNU7
25083.1
0.339
0.114
17
LNU138
14074.5
5.71
0.514
8


LNU125
25944.1
0.681
0.797
3
LNU8
25063.6
0.354
0.046
23
LNU157
24982.1
5.62
0.607
7


LNU138
14074.5
0.714
0.514
8
LNU8
25061.2
0.324
0.254
12
LNU178
14614.5
6.17
0.189
17


LNU157
24982.1
0.703
0.607
7
CONT.
—
0.352
—
0
LNU178
14611.5
5.80
0.430
10


LNU178
14614.5
0.771
0.189
17
LNU10
25123.5
0.379
0.378
8
LNU178
14612.1
5.63
0.595
7


LNU178
14611.5
0.725
0.430
10
LNU122
25332.2
0.399
0.125
13
LNU178
14611.4
5.56
0.664
6


LNU178
14612.1
0.704
0.595
7
LNU122
25332.1
0.394
0.177
12
LNU220
25405.6
5.86
0.398
11


LNU178
14611.4
0.695
0.664
6
LNU122
25332.5
0.388
0.241
10
LNU220
25405.5
5.83
0.409
11


LNU220
25405.6
0.732
0.398
11
LNU125
25944.1
0.370
0.592
5
LNU220
25405.1
5.79
0.438
10


LNU220
25405.5
0.729
0.409
11
LNU125
25943.2
0.367
0.611
4
LNU220
25405.2
5.59
0.633
6


LNU220
25405.1
0.723
0.438
10
LNU125
25941.2
0.367
0.639
4
LNU234
25014.4
5.62
0.621
7


LNU220
25405.2
0.699
0.633
6
LNU138
14074.5
0.393
0.180
12
LNU234
25014.6
5.50
0.728
5


LNU234
25014.4
0.703
0.621
7
LNU138
14071.5
0.376
0.424
7
LNU236
25424.2
6.84
0.028
30


LNU234
25014.6
0.688
0.728
5
LNU138
14074.6
0.371
0.526
5
LNU236
25422.4
5.91
0.336
12


LNU236
25424.2
0.855
0.028
30
LNU157
24982.1
0.399
0.137
13
LNU236
25425.4
5.82
0.420
11


LNU236
25422.4
0.739
0.336
12
LNU157
24983.3
0.365
0.675
4
LNU236
25423.3
5.76
0.479
9


LNU236
25425.4
0.728
0.420
11
LNU178
14611.5
0.382
0.336
8
LNU24
24974.2
5.55
0.672
5


LNU236
25423.3
0.720
0.479
9
LNU178
14614.5
0.381
0.325
8
LNU24
24972.1
5.45
0.786
4


LNU24
24974.2
0.694
0.672
5
LNU178
14611.1
0.370
0.552
5
LNU25
14082.8
6.62
0.055
26


LNU24
24972.1
0.681
0.786
4
LNU178
14611.4
0.366
0.648
4
LNU25
14082.9
5.75
0.484
9


LNU25
14082.8
0.827
0.055
26
LNU178
14612.1
0.363
0.717
3
LNU25
14084.6
5.55
0.670
6


LNU25
14082.9
0.719
0.484
9
LNU220
25405.2
0.382
0.336
8
LNU271
25911.4
5.93
0.358
13


LNU25
14084.6
0.694
0.670
6
LNU220
25405.5
0.376
0.427
7
LNU278
25814.1
5.78
0.448
10


LNU271
25911.4
0.741
0.358
13
LNU220
25405.6
0.367
0.635
4
LNU278
25814.3
5.50
0.720
5


LNU278
25814.1
0.722
0.448
10
LNU234
25014.6
0.368
0.595
5
LNU278
25812.2
5.47
0.754
4


LNU278
25814.3
0.688
0.720
5
LNU236
25424.2
0.423
0.025
20
LNU43
14423.7
5.68
0.545
8


LNU278
25812.2
0.684
0.754
4
LNU236
25423.3
0.379
0.418
8
LNU43
14422.9
5.46
0.777
4


LNU43
14423.7
0.709
0.545
8
LNU236
25422.4
0.366
0.629
4
LNU45
25052.12
7.19
0.008
36


LNU43
14422.9
0.683
0.777
4
LNU24
24972.1
0.372
0.522
6
LNU45
25053.4
6.71
0.048
27


LNU45
25052.12
0.898
0.008
36
LNU24
24971.2
0.365
0.689
4
LNU45
25052.11
5.94
0.317
13


LNU45
25053.4
0.839
0.048
27
LNU24
24974.2
0.364
0.696
3
LNU45
25052.9
5.75
0.471
9


LNU45
25052.11
0.742
0.317
13
LNU25
14082.8
0.395
0.154
12
LNU67
25824.3
6.41
0.104
22


LNU45
25052.9
0.719
0.471
9
LNU25
14082.9
0.393
0.184
12
LNU67
25824.5
5.89
0.354
12


LNU67
25824.3
0.801
0.104
22
LNU271
25911.4
0.388
0.243
10
LNU67
25821.4
5.70
0.521
8


LNU67
25824.5
0.736
0.354
12
LNU271
25912.1
0.362
0.743
3
LNU9
25001.1
6.13
0.201
16


LNU67
25821.4
0.713
0.521
8
LNU278
25813.2
0.382
0.340
8
LNU9
25001.7
6.03
0.271
15


LNU9
25001.1
0.767
0.201
16
LNU43
14422.9
0.379
0.417
8
LNU9
25003.1
5.56
0.670
6


LNU9
25001.7
0.754
0.271
15
LNU43
14423.7
0.377
0.421
7
CONT.
—
5.11
—
0


LNU9
25003.1
0.695
0.670
6
LNU43
14423.6
0.365
0.680
4
LNU10
25123.6
6.24
0.253
22


CONT.
—
0.638
—
0
LNU45
25052.12
0.407
0.076
16
LNU157
24982.8
6.83
0.093
34


LNU10
25123.6
0.779
0.253
22
LNU45
25053.4
0.397
0.161
13
LNU157
24982.4
5.78
0.506
13


LNU157
24982.8
0.853
0.093
34
LNU45
25052.9
0.368
0.599
4
LNU157
24983.3
5.44
0.736
7


LNU157
24982.4
0.723
0.506
13
LNU67
25824.3
0.395
0.165
12
LNU168
24751.2
5.47
0.732
7


LNU157
24983.3
0.680
0.736
7
LNU67
25824.5
0.393
0.173
12
LNU173
25451.1
6.15
0.322
20


LNU168
24751.2
0.683
0.732
7
LNU67
25821.4
0.368
0.597
5
LNU178
14611.4
7.38
0.047
45


LNU173
25451.1
0.768
0.322
20
LNU67
25821.5
0.364
0.712
3
LNU178
14611.5
6.73
0.122
32


LNU178
14611.4
0.923
0.047
45
LNU9
25001.7
0.379
0.381
8
LNU178
14611.1
6.59
0.187
29


LNU178
14611.5
0.842
0.122
32
LNU9
25001.2
0.378
0.396
7
LNU184
25393.2
6.42
0.202
26


LNU178
14611.1
0.823
0.187
29
LNU9
25003.1
0.370
0.566
5
LNU184
25393.3
6.25
0.281
22


LNU184
25393.2
0.803
0.202
26
LNU9
25001.1
0.368
0.580
5
LNU184
25393.1
6.04
0.411
18


LNU184
25393.3
0.782
0.281
22
CONT.
—
0.307
—
0
LNU184
25395.1
5.98
0.419
17


LNU184
25393.1
0.755
0.411
18
LNU157
24982.8
0.372
0.169
21
LNU230
25413.1
6.82
0.101
34


LNU184
25395.1
0.747
0.419
17
LNU157
24982.4
0.354
0.335
16
LNU230
25413.2
6.73
0.124
32


LNU230
25413.1
0.852
0.101
34
LNU157
24983.3
0.319
0.797
4
LNU230
25412.1
6.22
0.275
22


LNU230
25413.2
0.841
0.124
32
LNU168
24751.2
0.346
0.435
13
LNU230
25412.2
6.09
0.339
19


LNU230
25412.1
0.778
0.275
22
LNU173
25451.1
0.399
0.076
30
LNU230
25415.1
6.04
0.370
18


LNU230
25412.2
0.761
0.339
19
LNU173
25451.11
0.337
0.519
10
LNU236
25425.4
7.78
0.014
52


LNU230
25415.1
0.755
0.370
18
LNU173
25451.2
0.335
0.563
9
LNU236
25423.3
6.50
0.189
27


LNU236
25425.4
0.973
0.014
52
LNU178
14611.4
0.400
0.074
30
LNU236
25422.4
5.94
0.416
16


LNU236
25423.3
0.813
0.189
27
LNU178
14611.5
0.379
0.143
24
LNU236
25424.2
5.93
0.419
16


LNU236
25422.4
0.743
0.416
16
LNU178
14611.1
0.353
0.368
15
LNU24
24974.2
7.22
0.048
41


LNU236
25424.2
0.741
0.419
16
LNU184
25393.3
0.368
0.232
20
LNU24
24971.3
6.02
0.366
18


LNU24
24974.2
0.903
0.048
41
LNU184
25393.2
0.358
0.291
17
LNU263
25791.3
6.66
0.142
30


LNU24
24971.3
0.753
0.366
18
LNU184
25395.1
0.354
0.364
15
LNU263
25794.3
6.36
0.229
25


LNU263
25791.3
0.832
0.142
30
LNU184
25393.1
0.344
0.492
12
LNU263
25794.8
6.23
0.272
22


LNU263
25794.3
0.795
0.229
25
LNU184
25394.3
0.325
0.701
6
LNU263
25792.2
5.56
0.649
9


LNU263
25794.8
0.779
0.272
22
LNU20
24933.4
0.348
0.400
13
LNU276
25433.1
6.40
0.218
25


LNU263
25792.2
0.695
0.649
9
LNU230
25413.2
0.382
0.133
25
LNU276
25431.1
6.37
0.226
25


LNU276
25433.1
0.800
0.218
25
LNU230
25413.1
0.373
0.175
22
LNU276
25433.3
6.01
0.361
18


LNU276
25431.1
0.796
0.226
25
LNU230
25412.1
0.352
0.348
15
LNU279
25484.3
6.77
0.111
33


LNU276
25433.3
0.752
0.361
18
LNU230
25415.1
0.340
0.486
11
LNU279
25481.3
6.62
0.165
30


LNU279
25484.3
0.846
0.111
33
LNU230
25412.2
0.327
0.684
7
LNU279
25481.5
6.32
0.245
24


LNU279
25481.3
0.827
0.165
30
LNU236
25425.4
0.426
0.018
39
LNU279
25481.2
6.07
0.400
19


LNU279
25481.5
0.790
0.245
24
LNU236
25423.3
0.352
0.365
15
LNU279
25481.4
6.07
0.356
19


LNU279
25481.2
0.759
0.400
19
LNU236
25424.2
0.352
0.351
15
LNU36
25562.3
6.64
0.161
30


LNU279
25481.4
0.759
0.356
19
LNU236
25422.4
0.325
0.705
6
LNU36
25562.7
6.54
0.219
28


LNU36
25562.3
0.830
0.161
30
LNU24
24974.2
0.401
0.057
31
LNU36
25561.2
6.18
0.307
21


LNU36
25562.7
0.817
0.219
28
LNU24
24971.3
0.361
0.263
18
LNU56
24694.1
6.66
0.177
31


LNU36
25561.2
0.772
0.307
21
LNU24
24971.4
0.348
0.467
14
LNU56
24693.1
6.41
0.228
25


LNU56
24694.1
0.833
0.177
31
LNU24
24971.2
0.323
0.729
5
LNU56
24691.2
6.26
0.267
23


LNU56
24693.1
0.801
0.228
25
LNU263
25791.3
0.362
0.271
18
LNU56
24694.2
5.66
0.598
11


LNU56
24691.2
0.783
0.267
23
LNU263
25794.8
0.361
0.260
18
LNU73
25755.1
7.76
0.015
52


LNU56
24694.2
0.707
0.598
11
LNU263
25794.3
0.354
0.328
15
LNU73
25751.1
6.71
0.120
31


LNU73
25755.1
0.970
0.015
52
LNU263
25794.6
0.341
0.506
11
LNU73
25751.9
6.57
0.163
29


LNU73
25751.1
0.839
0.120
31
LNU276
25433.1
0.382
0.135
25
LNU73
25751.8
6.32
0.271
24


LNU73
25751.9
0.822
0.163
29
LNU276
25431.1
0.374
0.174
22
LNU73
25754.2
6.25
0.291
22


LNU73
25751.8
0.790
0.271
24
LNU276
25433.3
0.346
0.409
13
LNU9
25001.7
6.80
0.115
33


LNU73
25754.2
0.782
0.291
22
LNU276
25433.2
0.338
0.534
10
LNU9
25001.1
6.51
0.177
27


LNU9
25001.7
0.851
0.115
33
LNU279
25481.3
0.372
0.202
21
LNU9
25001.2
6.05
0.356
18


LNU9
25001.1
0.813
0.177
27
LNU279
25484.3
0.367
0.215
20
CONT.
—
3.57
—
0


LNU9
25001.2
0.756
0.356
18
LNU279
25481.4
0.355
0.331
16
LNU131
14005.5
7.37
0.000
106


CONT.
—
0.451
—
0
LNU279
25481.2
0.346
0.475
13
LNU131
14005.2
5.32
0.050
49


LNU131
14005.5
0.921
0.000
104
LNU279
25481.5
0.341
0.485
11
LNU131
14002.15
4.88
0.135
36


LNU131
14005.2
0.665
0.059
47
LNU36
25562.3
0.393
0.090
28
LNU135
26204.2
7.67
0.000
115


LNU131
14002.15
0.609
0.154
35
LNU36
25561.2
0.371
0.195
21
LNU135
26203.6
5.27
0.052
48


LNU135
26204.2
0.959
0.000
113
LNU36
25562.7
0.361
0.323
18
LNU135
26203.4
5.22
0.074
46


LNU135
26203.6
0.659
0.061
46
LNU36
25562.4
0.331
0.618
8
LNU135
26203.1
4.95
0.109
39


LNU135
26203.4
0.653
0.085
45
LNU36
25562.9
0.321
0.763
5
LNU135
26203.3
4.18
0.494
17


LNU135
26203.1
0.619
0.126
37
LNU53
25674.1
0.328
0.660
7
LNU161
14553.5
5.35
0.060
50


LNU135
26203.3
0.522
0.528
16
LNU53
25674.3
0.324
0.716
6
LNU161
14553.6
4.33
0.372
21


LNU161
14553.5
0.669
0.069
48
LNU53
25674.6
0.322
0.759
5
LNU173
25451.2
4.03
0.583
13


LNU161
14553.6
0.541
0.404
20
LNU56
24694.1
0.381
0.171
24
LNU173
25451.5
3.89
0.709
9


LNU173
25451.2
0.504
0.620
12
LNU56
24693.1
0.372
0.204
21
LNU181
25771.2
5.43
0.036
52


LNU173
25451.5
0.486
0.746
8
LNU56
24691.2
0.368
0.220
20
LNU181
25771.11
4.74
0.176
33


LNU181
25771.2
0.679
0.044
51
LNU56
24694.2
0.343
0.477
12
LNU181
25771.6
4.74
0.171
33


LNU181
25771.11
0.593
0.198
31
LNU73
25755.1
0.405
0.051
32
LNU181
25774.1
4.36
0.350
22


LNU181
25771.6
0.593
0.194
31
LNU73
25751.1
0.387
0.103
26
LNU181
25771.8
3.81
0.783
7


LNU181
25774.1
0.545
0.382
21
LNU73
25751.8
0.379
0.165
24
LNU184
25395.1
6.17
0.006
73


LNU184
25395.1
0.771
0.007
71
LNU73
25754.2
0.378
0.171
23
LNU184
25394.3
6.00
0.010
68


LNU184
25394.3
0.750
0.012
66
LNU73
25751.9
0.377
0.145
23
LNU184
25394.1
5.43
0.040
52


LNU184
25394.1
0.678
0.047
50
LNU9
25001.1
0.374
0.163
22
LNU184
25393.3
5.24
0.057
47


LNU184
25393.3
0.655
0.068
45
LNU9
25001.7
0.370
0.207
21
LNU184
25393.2
4.23
0.436
18


LNU184
25393.2
0.529
0.470
17
LNU9
25001.2
0.350
0.362
14
LNU184
25393.1
4.19
0.472
17


LNU184
25393.1
0.524
0.506
16
LNU9
25001.3
0.326
0.687
6
LNU224
25871.3
5.98
0.008
67


LNU224
25871.3
0.747
0.010
66
LNU9
25003.1
0.325
0.718
6
LNU224
25874.1
5.87
0.010
64


LNU224
25874.1
0.734
0.013
63
CONT.
—
0.287
—
0
LNU224
25872.2
5.35
0.047
50


LNU224
25872.2
0.669
0.056
48
LNU131
14005.5
0.396
0.020
38
LNU224
25874.4
4.53
0.266
27


LNU224
25872.3
0.601
0.174
33
LNU131
14005.2
0.353
0.165
23
LNU224
25872.3
4.51
0.278
26


LNU224
25874.4
0.567
0.294
26
LNU131
14002.15
0.328
0.376
14
LNU246
25743.2
5.97
0.014
67


LNU246
25743.2
0.747
0.017
66
LNU135
26204.2
0.437
0.003
52
LNU246
25743.1
5.65
0.024
58


LNU246
25743.1
0.707
0.029
57
LNU135
26203.4
0.343
0.244
20
LNU246
25744.2
5.49
0.039
54


LNU246
25744.2
0.687
0.046
52
LNU135
26203.6
0.336
0.290
17
LNU246
25744.4
5.08
0.111
42


LNU246
25744.4
0.635
0.126
41
LNU135
26203.1
0.315
0.540
10
LNU246
25744.3
4.48
0.282
26


LNU246
25744.3
0.561
0.311
24
LNU135
26203.3
0.310
0.641
8
LNU250
25592.2
5.37
0.043
50


LNU250
25592.2
0.672
0.051
49
LNU161
14553.5
0.320
0.500
11
LNU250
25592.1
4.28
0.412
20


LNU250
25592.1
0.535
0.445
19
LNU181
25771.2
0.339
0.265
18
LNU250
25591.1
4.28
0.410
20


LNU250
25591.1
0.535
0.444
18
LNU181
25771.6
0.327
0.380
14
LNU260
26404.8
4.71
0.191
32


LNU260
26404.8
0.589
0.213
31
LNU181
25771.11
0.318
0.505
11
LNU260
26404.7
4.63
0.238
30


LNU260
26404.7
0.579
0.263
28
LNU181
25774.1
0.309
0.638
7
LNU260
26404.1
4.46
0.311
25


LNU260
26404.1
0.558
0.340
24
LNU181
25771.8
0.299
0.795
4
LNU260
26403.1
3.81
0.777
7


LNU276
25433.6
0.602
0.175
33
LNU184
25395.1
0.377
0.063
31
LNU276
25433.6
4.82
0.154
35


LNU276
25431.1
0.542
0.404
20
LNU184
25394.3
0.362
0.119
26
LNU276
25431.1
4.33
0.371
21


LNU276
25433.5
0.532
0.459
18
LNU184
25394.1
0.333
0.318
16
LNU276
25433.5
4.26
0.426
19


LNU279
25484.3
0.667
0.061
48
LNU184
25393.3
0.330
0.355
15
LNU279
25484.3
5.34
0.052
49


LNU279
25481.3
0.604
0.202
34
LNU184
25393.1
0.304
0.714
6
LNU279
25481.3
4.83
0.182
35


LNU279
25481.5
0.568
0.292
26
LNU184
25393.2
0.303
0.726
6
LNU279
25481.5
4.54
0.265
27


LNU279
25481.4
0.525
0.497
16
LNU224
25871.3
0.367
0.086
28
LNU279
25481.4
4.20
0.463
18


LNU3
26122.2
0.614
0.157
36
LNU224
25872.2
0.364
0.104
27
LNU3
26122.2
4.91
0.139
37


LNU33
25553.3
0.686
0.040
52
LNU224
25874.1
0.355
0.138
24
LNU33
25553.3
5.48
0.034
54


LNU33
25553.2
0.615
0.142
36
LNU224
25872.3
0.315
0.548
10
LNU33
25552.2
4.78
0.197
34


LNU33
25552.2
0.598
0.218
32
LNU246
25743.2
0.369
0.100
29
LNU33
25553.2
4.57
0.246
28


LNU33
25553.1
0.522
0.527
16
LNU246
25743.1
0.354
0.164
23
LNU33
25553.1
4.18
0.493
17


LNU53
25674.6
0.546
0.383
21
LNU246
25744.2
0.347
0.216
21
LNU53
25674.6
4.37
0.352
22


LNU73
25751.8
0.521
0.539
16
LNU246
25744.4
0.344
0.256
20
LNU73
25751.8
4.17
0.506
17


CONT.
—
0.809
—
0
LNU246
25744.3
0.313
0.570
9
CONT.
—
6.47
—
0


LNU119
26144.2.
0.861
0.646
6
LNU250
25592.2
0.359
0.121
25
LNU119
26144.2.
6.89
0.646
6


LNU130
24912.7.
0.932
0.319
15
LNU250
25592.1
0.308
0.649
7
LNU130
24912.7.
7.45
0.319
15


LNU130
24913.6.
0.926
0.303
14
LNU250
25591.1
0.301
0.772
5
LNU130
24913.6.
7.41
0.303
14


LNU130
24914.5.
0.893
0.475
10
LNU260
26404.7
0.321
0.485
12
LNU130
24914.5.
7.14
0.475
10


LNU130
24911.7.
0.860
0.661
6
LNU260
26404.8
0.316
0.529
10
LNU130
24911.7.
6.88
0.661
6


LNU136
14511.10.
1.025
0.060
27
LNU276
25433.6
0.332
0.330
16
LNU136
14511.10.
8.20
0.060
27


LNU136
14515.5.
0.969
0.180
20
LNU276
25431.1
0.302
0.738
5
LNU136
14515.5.
7.75
0.180
20


LNU142
27541.1.
0.946
0.231
17
LNU279
25481.3
0.346
0.238
21
LNU142
27541.1.
7.57
0.231
17


LNU149
26175.3.
0.901
0.415
11
LNU279
25484.3
0.345
0.234
20
LNU149
26175.3.
6.73
0.773
4


LNU15
14123.13.
0.904
0.411
12
LNU279
25481.5
0.323
0.444
12
LNU15
14123.13.
7.23
0.411
12


LNU15
14123.11.
0.901
0.455
11
LNU279
25481.4
0.310
0.615
8
LNU15
14123.11.
6.81
0.737
5


LNU185
26474.2.
1.021
0.089
26
LNU3
26122.2
0.336
0.309
17
LNU185
26474.2.
8.17
0.089
26


LNU185
26475.1.
0.982
0.132
21
LNU33
25553.3
0.364
0.103
27
LNU185
26475.1.
7.86
0.132
21


LNU185
26474.1.
0.893
0.487
10
LNU33
25552.2
0.339
0.288
18
LNU185
26474.1.
7.14
0.487
10


LNU212
25833.2.
1.095
0.020
35
LNU33
25553.2
0.329
0.365
14
LNU212
25833.2.
8.76
0.020
35


LNU212
25834.4.
1.035
0.067
28
LNU33
25553.1
0.320
0.487
11
LNU212
25834.4.
8.28
0.067
28


LNU212
25834.5.
0.941
0.254
16
LNU53
25674.6
0.311
0.601
8
LNU212
25834.5.
7.53
0.254
16


LNU212
25832.1.
0.863
0.630
7
LNU56
24694.2
0.303
0.733
5
LNU212
25832.1.
6.91
0.630
7


LNU216
25985.4.
1.045
0.062
29
LNU73
25751.8
0.329
0.404
14
LNU216
25985.4.
8.36
0.062
29


LNU216
25984.1.
1.010
0.104
25
CONT.
—
0.359
—
0
LNU216
25984.1.
8.08
0.104
25


LNU216
25982.1.
1.005
0.090
24
LNU119
26144.2.
0.399
0.196
11
LNU216
25982.1.
8.04
0.090
24


LNU216
25984.6.
0.878
0.537
9
LNU130
24912.7.
0.417
0.098
16
LNU216
25984.6.
7.02
0.537
9


LNU228
26224.7.
0.989
0.123
22
LNU130
24911.7.
0.407
0.136
13
LNU228
26224.7.
7.91
0.123
22


LNU228
26222.4.
0.907
0.385
12
LNU130
24914.5.
0.398
0.243
11
LNU228
26222.4.
7.26
0.385
12


LNU229
26112.3.
0.956
0.208
18
LNU130
24913.6.
0.378
0.541
5
LNU229
26112.3.
7.65
0.208
18


LNU253
26241.1.
0.935
0.281
16
LNU136
14511.10.
0.415
0.062
15
LNU253
26241.1.
7.48
0.281
16


LNU280
26162.1.
0.941
0.274
16
LNU136
14515.5.
0.388
0.397
8
LNU280
26162.1.
7.53
0.274
16


LNU55
26015.1.
0.911
0.389
13
LNU136
14514.8.
0.378
0.553
5
LNU55
26015.1.
7.28
0.389
13


LNU81
26034.2.
0.891
0.476
10
LNU136
14515.1.
0.369
0.761
3
LNU81
26034.2.
7.13
0.476
10


LNU81
26031.9.
0.891
0.493
10
LNU142
27541.1.
0.391
0.310
9
LNU81
26031.9.
7.13
0.493
10


CONT.
—
0.822
—
0
LNU149
26175.3.
0.393
0.257
9
CONT.
—
6.58
—
0


LNU119
26141.1.
1.016
0.363
24
LNU149
26175.1.
0.373
0.647
4
LNU119
26141.1.
8.13
0.363
24


LNU119
26142.5.
0.876
0.777
7
LNU15
14123.13.
0.399
0.197
11
LNU119
26142.5.
7.01
0.777
7


LNU130
24913.5.
0.946
0.514
15
LNU15
14123.11.
0.387
0.443
8
LNU130
24913.5.
7.57
0.514
15


LNU130
24914.5.
0.903
0.668
10
LNU15
14122.9.
0.374
0.645
4
LNU130
24914.5.
7.22
0.668
10


LNU136
14515.1.
0.892
0.711
8
LNU185
26474.2.
0.414
0.133
15
LNU136
14515.1.
7.13
0.711
8


LNU142
27546.2.
0.902
0.680
10
LNU185
26475.1.
0.399
0.193
11
LNU142
27546.2.
7.22
0.680
10


LNU142
27545.1.
0.876
0.780
7
LNU185
26474.1.
0.399
0.242
11
LNU142
27545.1.
7.01
0.780
7


LNU15
14124.12.
0.984
0.405
20
LNU212
25833.2.
0.436
0.019
21
LNU15
14124.12.
7.87
0.405
20


LNU15
14122.8.
0.942
0.541
15
LNU212
25834.4.
0.435
0.026
21
LNU15
14122.8.
7.54
0.541
15


LNU15
14123.13.
0.904
0.666
10
LNU212
25834.5.
0.431
0.028
20
LNU15
14123.13.
7.23
0.666
10


LNU185
26474.1.
1.074
0.194
31
LNU212
25834.1.
0.404
0.167
13
LNU185
26474.1.
8.59
0.194
31


LNU212
25834.4.
1.045
0.255
27
LNU212
25832.1.
0.391
0.298
9
LNU212
25834.4.
8.36
0.255
27


LNU212
25834.5.
0.924
0.596
12
LNU216
25984.1.
0.448
0.011
25
LNU212
25834.5.
7.39
0.596
12


LNU216
25985.4.
1.096
0.186
33
LNU216
25982.1.
0.445
0.005
24
LNU216
25985.4.
8.77
0.186
33


LNU216
25982.1.
0.951
0.499
16
LNU216
25985.4.
0.425
0.060
18
LNU216
25982.1.
7.61
0.499
16


LNU216
25984.6.
0.925
0.610
12
LNU216
25984.6.
0.400
0.175
11
LNU216
25984.6.
7.40
0.610
12


LNU228
26225.2.
1.099
0.161
34
LNU228
26222.4.
0.401
0.170
12
LNU228
26225.2.
8.79
0.161
34


LNU228
26224.7.
1.018
0.337
24
LNU228
26224.7.
0.385
0.414
7
LNU228
26224.6.
7.80
0.434
19


LNU228
26224.6.
0.974
0.434
19
LNU228
26224.6.
0.383
0.450
7
LNU228
26222.4.
7.58
0.509
15


LNU228
26222.4.
0.948
0.509
15
LNU228
26222.1.
0.379
0.588
5
LNU228
26222.1.
7.54
0.528
15


LNU228
26222.1.
0.943
0.528
15
LNU229
26112.3.
0.419
0.057
17
LNU229
26111.7.
8.25
0.318
25


LNU229
26111.7.
1.031
0.318
25
LNU229
26112.6.
0.371
0.710
3
LNU229
26111.5.
8.05
0.348
22


LNU229
26111.5.
1.006
0.348
22
LNU253
26241.1.
0.402
0.172
12
LNU229
26112.4.
7.87
0.406
20


LNU229
26112.4.
0.984
0.406
20
LNU253
26245.1.
0.385
0.402
7
LNU229
26112.3.
7.83
0.423
19


LNU229
26112.3.
0.978
0.423
19
LNU274
26264.2.
0.383
0.481
7
LNU241
26232.4.
7.72
0.458
17


LNU241
26232.4.
0.965
0.458
17
LNU274
26263.2.
0.372
0.661
4
LNU241
26233.3.
7.50
0.574
14


LNU241
26233.3.
0.938
0.574
14
LNU280
26162.1.
0.404
0.172
13
LNU241
26234.1.
7.10
0.729
8


LNU241
26234.1.
0.888
0.729
8
LNU280
26164.4.
0.367
0.797
2
LNU253
26242.1.
7.19
0.695
9


LNU253
26242.1.
0.899
0.695
9
LNU55
26015.1.
0.382
0.493
6
LNU274
26265.1.
7.39
0.618
12


LNU274
26265.1.
0.923
0.618
12
LNU81
26031.9.
0.378
0.557
5
LNU274
26262.2.
7.31
0.646
11


LNU274
26262.2.
0.913
0.646
11
LNU81
26034.3.
0.373
0.646
4
LNU280
26162.1.
7.93
0.386
21


LNU280
26162.1.
0.992
0.386
21
CONT.
—
0.401
—
0
LNU55
26013.3.
7.44
0.609
13


LNU280
26164.4.
0.904
0.662
10
LNU119
26141.1.
0.431
0.688
8
LNU55
26015.1.
7.37
0.602
12


LNU55
26013.3.
0.930
0.609
13
LNU130
24912.7.
0.420
0.792
5
LNU55
26013.4.
7.34
0.613
12


LNU55
26015.1.
0.922
0.602
12
LNU15
14122.8.
0.431
0.661
8
LNU81
26031.10.
7.95
0.440
21


LNU55
26013.4.
0.917
0.613
12
LNU15
14124.12.
0.425
0.727
6
LNU81
26031.2.
7.50
0.541
14


LNU81
26031.10.
0.994
0.440
21
LNU185
26474.1.
0.469
0.311
17







LNU81
26031.2.
0.937
0.541
14
LNU212
25834.4.
0.443
0.521
11












LNU216
25985.4.
0.437
0.596
9












LNU216
25982.1.
0.424
0.725
6












LNU228
26224.7.
0.460
0.397
15












LNU228
26225.2.
0.456
0.410
14












LNU228
26224.6.
0.428
0.686
7












LNU229
26112.3.
0.463
0.366
16












LNU229
26111.7.
0.455
0.448
13












LNU229
26111.5.
0.418
0.794
4












LNU241
26232.4.
0.460
0.380
15












LNU241
26233.3.
0.445
0.562
11












LNU253
26241.1.
0.424
0.720
6












LNU274
26262.2.
0.431
0.660
8












LNU274
26263.2.
0.421
0.764
5












LNU280
26162.1.
0.438
0.582
9












LNU280
26164.4.
0.424
0.725
6












LNU55
26013.3.
0.448
0.529
12












LNU81
26031.10.
0.432
0.675
8












LNU81
26034.3.
0.426
0.732
6





Table 81.


“CONT.”—Control;


“Ave.”—Average;


“% Incr.” = % increment.






The genes listed in Tables 82 and 83 improved plant NUE when grown at standard nitrogen concentration levels. These genes produced larger plants with a larger photosynthetic area and increased biomass (fresh weight, dry weight, rosette diameter, rosette area and plot coverage) when grown under standard nitrogen conditions. The genes were cloned under the regulation of a constitutive (At6669) and root preferred promoter (RootP). The evaluation of each gene was performed by testing the performance of different number of events. Event with p-value <0.1 was considered statistically significant.







TABLE 82







Genes showing improved plant biomass production


at standard nitrogen growth conditions











Dry Weight [g]

Fresh Weight [g]
















Gene


P-
%
Gene


P-
%


Name
Event #
Ave.
Value
incr.
Name
Event #
Ave.
Value
incr.



















CONT.
—
0.241
—
0
CONT.
—
2.503
—
0


LNU100
14473.3
0.264
0.319
9
LNU104
25033.1
2.750
0.418
10


LNU104
25033.1
0.279
0.272
16
LNU104
25032.2
2.563
0.745
2


LNU104
25032.2
0.273
0.000
13
LNU106
14484.3
3.125
0.148
25


LNU104
25033.3
0.259
0.039
7
LNU114
25044.4
2.800
0.512
12


LNU104
25034.1
0.253
0.337
5
LNU114
25044.3
2.788
0.003
11


LNU106
14484.3
0.314
0.221
30
LNU114
25041.1
2.681
0.625
7


LNU106
14482.3
0.279
0.125
16
LNU155
14524.8
3.067
0.330
23


LNU106
14483.5
0.251
0.655
4
LNU155
14525.6
2.706
0.290
8


LNU114
25041.1
0.260
0.304
8
LNU218
24781.2
2.750
0.168
10


LNU114
25044.3
0.253
0.075
5
LNU218
24781.1
2.738
0.261
9


LNU114
25044.4
0.253
0.698
5
LNU218
24781.4
2.563
0.437
2


LNU114
25042.1
0.246
0.762
2
LNU28
25174.5
2.625
0.650
5


LNU155
14524.8
0.288
0.471
19
LNU4
25134.1
2.800
0.582
12


LNU155
14525.6
0.258
0.146
7
LNU40
24794.4
2.756
0.006
10


LNU213
24653.2
0.277
0.088
15
LNU48
24801.4
2.656
0.741
6


LNU218
24781.7
0.319
0.224
32
LNU63
24811.2
2.704
0.336
8


LNU218
24781.2
0.271
0.380
12
LNU7
25083.3
3.006
0.323
20


LNU218
24781.1
0.256
0.113
6
LNU8
25062.2
2.656
0.200
6


LNU218
24781.4
0.252
0.389
4
LNU8
25063.1
2.631
0.691
5


LNU23
25163.5
0.283
0.597
17
LNU8
25061.2
2.594
0.722
4


LNU23
25163.4
0.263
0.493
9
LNU94
24833.3
2.675
0.541
7


LNU28
25171.2
0.275
0.429
14
CONT.
—
2.638
—
0


LNU28
25174.3
0.267
0.526
11
LNU132
14102.6
2.794
0.720
6


LNU28
25174.5
0.251
0.251
4
LNU65
24703.1
3.006
0.684
14


LNU28
25171.4
0.249
0.762
3
CONT.
—
2.303
—
0


LNU4
25134.1
0.281
0.255
17
LNU113
25631.1
2.631
0.213
14


LNU4
25134.2
0.253
0.690
5
LNU113
25631.3
2.500
0.261
9


LNU4
25133.3
0.248
0.681
3
LNU120
25463.7
2.500
0.059
9


LNU40
24794.3
0.260
0.212
8
LNU120
25464.1
2.344
0.420
2


LNU40
24794.4
0.259
0.435
7
LNU148
25685.2
2.719
0.315
18


LNU46
14462.1
0.273
0.121
13
LNU148
25683.2
2.544
0.533
10


LNU48
24802.2
0.281
0.000
16
LNU148
25685.1
2.519
0.537
9


LNU48
24801.4
0.268
0.360
11
LNU287
24674.3
2.419
0.730
5


LNU48
24802.1
0.258
0.018
7
LNU287
24674.2
2.331
0.726
1


LNU63
24811.2
0.285
0.475
18
LNU37
14064.7
2.719
0.186
18


LNU63
24814.2
0.262
0.293
9
LNU37
14064.1
2.575
0.275
12


LNU7
25083.3
0.357
0.496
48
LNU37
14064.6
2.375
0.637
3


LNU7
25083.1
0.248
0.326
3
LNU5
14043.7
2.750
0.000
19


LNU8
25062.2
0.268
0.001
11
LNU65
24702.3
2.644
0.025
15


LNU8
25063.1
0.259
0.403
7
LNU65
24703.3
2.400
0.393
4


LNU8
25061.2
0.249
0.759
3
LNU65
24703.6
2.381
0.593
3


LNU94
24833.3
0.273
0.455
13
LNU68
14034.13
2.569
0.000
12


LNU94
24834.4
0.247
0.369
2
LNU68
14033.1
2.413
0.457
5


LNU94
24831.4
0.244
0.781
1
LNU68
14034.1
2.394
0.544
4


LNU96
25073.4
0.279
0.590
16
LNU71
25853.1
2.613
0.152
13


CONT.
—
0.302
—
0
LNU71
25851.4
2.594
0.213
13


LNU132
14102.6
0.431
0.245
43
LNU71
25853.4
2.475
0.565
7


LNU140
14114.8
0.331
0.380
10
LNU71
25852.4
2.469
0.376
7


LNU148
25685.1
0.339
0.184
12
LNU71
25852.5
2.456
0.362
7


LNU287
24674.6
0.346
0.699
15
LNU72
24962.3
2.575
0.213
12


LNU5
14043.9
0.334
0.186
11
LNU72
24963.7
2.450
0.527
6


LNU65
24703.7
0.418
0.117
38
LNU72
24962.2
2.406
0.650
4


LNU65
24702.3
0.351
0.681
16
LNU72
24963.8
2.344
0.711
2


LNU71
25851.4
0.419
0.686
39
LNU74
25444.1
2.575
0.000
12


LNU98
25761.6
0.369
0.365
22
LNU74
25443.3
2.569
0.202
12


CONT.
—
0.243
—
0
LNU74
25443.2
2.525
0.363
10


LNU113
25631.1
0.269
0.122
11
LNU82
24823.1
2.538
0.000
10


LNU113
25631.3
0.261
0.116
7
LNU84
25621.2
2.413
0.499
5


LNU148
25685.2
0.283
0.365
16
LNU84
25621.6
2.356
0.674
2


LNU148
25683.2
0.261
0.583
7
LNU84
25623.1
2.350
0.724
2


LNU148
25685.1
0.261
0.416
7
CONT.
—
1.523
—
0


LNU287
24674.3
0.252
0.757
4
LNU117
25931.2
1.844
0.247
21


LNU37
14064.1
0.275
0.009
13
LNU117
25931.1
1.806
0.190
19


LNU37
14064.7
0.271
0.296
11
LNU117
25931.4
1.750
0.384
15


LNU5
14043.7
0.269
0.003
11
LNU117
25932.4
1.731
0.227
14


LNU65
24702.3
0.266
0.007
9
LNU122
25332.1
1.725
0.581
13


LNU65
24703.6
0.250
0.621
3
LNU122
25332.5
1.706
0.380
12


LNU65
24703.3
0.249
0.443
2
LNU122
25333.1
1.700
0.012
12


LNU68
14034.13
0.276
0.000
14
LNU122
25332.2
1.694
0.015
11


LNU68
14033.1
0.258
0.350
6
LNU122
25333.2
1.681
0.600
10


LNU71
25853.1
0.278
0.131
14
LNU125
25944.3
1.588
0.593
4


LNU71
25851.4
0.271
0.002
12
LNU125
25943.3
1.569
0.430
3


LNU71
25852.5
0.264
0.501
9
LNU138
14074.5
1.738
0.103
14


LNU71
25853.4
0.259
0.473
7
LNU138
14074.6
1.644
0.551
8


LNU71
25852.4
0.249
0.521
2
LNU138
14072.8
1.619
0.366
6


LNU72
24962.3
0.252
0.757
4
LNU180
24722.2
1.625
0.095
7


LNU72
24963.7
0.251
0.739
3
LNU230
25413.1
1.775
0.510
17


LNU72
24963.8
0.250
0.621
3
LNU230
25412.2
1.638
0.267
8


LNU72
24962.2
0.249
0.666
3
LNU254
25781.3
1.638
0.054
8


LNU74
25444.1
0.259
0.174
7
LNU254
25783.1
1.594
0.173
5


LNU74
25443.2
0.256
0.627
5
LNU263
25791.3
1.544
0.673
1


LNU74
25443.3
0.252
0.624
4
LNU271
25912.1
1.588
0.534
4


LNU82
24823.1
0.272
0.080
12
LNU278
25814.3
1.581
0.244
4


LNU82
24823.3
0.248
0.746
2
LNU43
14422.8
1.625
0.230
7


LNU84
25621.2
0.261
0.351
7
LNU43
14423.6
1.594
0.327
5


LNU84
25623.1
0.249
0.666
3
LNU43
14423.7
1.569
0.370
3


LNU87
24713.2
0.247
0.743
2
LNU45
25053.4
1.613
0.105
6


CONT.
—
0.162
—
0
LNU67
25824.5
1.688
0.319
11


LNU117
25931.4
0.200
0.003
24
CONT.
—
2.439
—
0


LNU117
25931.2
0.193
0.055
20
LNU100
14472.1
2.956
0.299
21


LNU117
25931.1
0.192
0.094
19
LNU100
14473.1
2.763
0.147
13


LNU117
25932.4
0.181
0.091
12
LNU100
14473.3
2.613
0.002
7


LNU117
25933.3
0.175
0.554
8
LNU104
25032.2
2.944
0.331
21


LNU122
25332.2
0.190
0.009
18
LNU104
25033.1
2.581
0.465
6


LNU122
25332.1
0.189
0.434
17
LNU104
25033.3
2.544
0.030
4


LNU122
25332.5
0.186
0.008
15
LNU104
25033.8
2.544
0.567
4


LNU122
25333.1
0.184
0.013
14
LNU104
25032.1
2.519
0.358
3


LNU122
25333.2
0.181
0.367
12
LNU106
14483.2
3.163
0.391
30


LNU125
25944.3
0.183
0.192
13
LNU106
14484.3
2.856
0.330
17


LNU125
25943.3
0.175
0.148
8
LNU114
25044.11
2.731
0.403
12


LNU125
25941.4
0.169
0.592
5
LNU114
25041.2
2.713
0.374
11


LNU138
14074.5
0.196
0.013
22
LNU114
25041.1
2.688
0.036
10


LNU138
14074.6
0.186
0.348
15
LNU114
25042.1
2.688
0.499
10


LNU180
24723.1
0.173
0.655
7
LNU114
25044.4
2.519
0.700
3


LNU180
24722.2
0.171
0.132
6
LNU117
25931.4
2.669
0.000
9


LNU180
24721.2
0.164
0.691
1
LNU117
25932.4
2.631
0.249
8


LNU220
25405.1
0.163
0.798
1
LNU117
25931.1
2.538
0.536
4


LNU230
25413.1
0.196
0.390
22
LNU117
25931.2
2.525
0.541
4


LNU230
25412.2
0.176
0.287
9
LNU155
14523.5
2.644
0.232
8


LNU230
25413.2
0.164
0.795
2
LNU155
14525.1
2.550
0.344
5


LNU25
14083.1
0.176
0.287
9
LNU180
24722.4
2.700
0.153
11


LNU254
25781.3
0.179
0.048
11
LNU180
24724.1
2.582
0.687
6


LNU254
25783.1
0.170
0.585
5
LNU180
24723.1
2.531
0.214
4


LNU263
25791.3
0.178
0.331
10
LNU218
24781.4
2.731
0.244
12


LNU263
25794.8
0.178
0.038
10
LNU218
24781.1
2.631
0.249
8


LNU263
25794.6
0.173
0.405
7
LNU218
24781.6
2.606
0.004
7


LNU267
25804.3
0.166
0.772
3
LNU254
25782.4
3.331
0.467
37


LNU271
25912.1
0.178
0.061
10
LNU254
25782.5
2.950
0.570
21


LNU271
25913.3
0.166
0.469
3
LNU254
25781.5
2.630
0.090
8


LNU278
25814.3
0.173
0.086
7
LNU254
25783.1
2.600
0.368
7


LNU278
25813.2
0.166
0.606
3
LNU4
25134.2
2.688
0.009
10


LNU36
25562.3
0.171
0.448
6
LNU4
25134.3
2.650
0.000
9


LNU36
25561.2
0.167
0.414
3
LNU4
25133.3
2.519
0.110
3


LNU43
14423.6
0.180
0.176
11
LNU40
24792.2
2.881
0.134
18


LNU43
14422.8
0.178
0.031
10
LNU40
24794.3
2.763
0.281
13


LNU43
14423.7
0.173
0.534
7
LNU40
24793.1
2.744
0.513
12


LNU43
14421.1
0.169
0.552
5
LNU40
24792.1
2.681
0.058
10


LNU45
25053.4
0.172
0.147
6
LNU46
14462.5
2.588
0.247
6


LNU45
25052.9
0.169
0.734
4
LNU46
14463.2
2.563
0.086
5


LNU67
25821.5
0.182
0.016
13
LNU46
14462.1
2.525
0.064
4


LNU67
25824.5
0.181
0.019
12
LNU46
14464.4
2.500
0.245
3


CONT.
—
0.256
—
0
LNU48
24801.4
3.004
0.165
23


LNU100
14472.1
0.345
0.295
35
LNU48
24803.2
2.931
0.241
20


LNU100
14473.1
0.279
0.113
9
LNU48
24804.4
2.625
0.001
8


LNU100
14473.3
0.272
0.724
6
LNU48
24802.2
2.575
0.669
6


LNU104
25032.2
0.361
0.403
41
LNU48
24802.1
2.538
0.235
4


LNU106
14483.2
0.414
0.483
62
LNU63
24814.7
2.806
0.166
15


LNU106
14484.3
0.363
0.263
42
LNU63
24812.2
2.663
0.267
9


LNU114
25044.11
0.358
0.468
40
LNU63
24812.3
2.575
0.332
6


LNU114
25042.1
0.316
0.479
24
LNU63
24814.2
2.531
0.051
4


LNU114
25041.2
0.280
0.462
9
LNU7
25083.3
2.725
0.360
12


LNU114
25041.1
0.266
0.507
4
LNU7
25081.1
2.713
0.006
11


LNU117
25931.2
0.292
0.022
14
LNU7
25083.1
2.700
0.409
11


LNU117
25931.4
0.279
0.113
9
LNU8
25062.1
2.633
0.253
8


LNU117
25931.1
0.268
0.394
5
LNU8
25061.2
2.550
0.112
5


LNU117
25932.4
0.264
0.686
3
LNU94
24831.4
2.704
0.083
11


LNU155
14523.5
0.264
0.686
3
LNU94
24833.3
2.613
0.002
7


LNU180
24722.4
0.315
0.247
23
LNU94
24834.1
2.538
0.738
4


LNU180
24724.1
0.283
0.759
11
LNU96
25071.2
2.725
0.315
12


LNU180
24723.1
0.282
0.353
10
LNU96
25073.4
2.681
0.351
10


LNU218
24781.4
0.308
0.558
20
LNU96
25071.3
2.525
0.690
4


LNU218
24781.1
0.271
0.342
6
LNU96
25074.1
2.506
0.497
3


LNU254
25782.4
0.448
0.464
75
CONT.
—
2.048
—
0


LNU254
25782.5
0.377
0.491
47
LNU10
25123.6
2.244
0.002
10


LNU254
25781.5
0.282
0.573
10
LNU10
25123.5
2.200
0.009
7


LNU254
25781.3
0.262
0.655
2
LNU122
25332.1
2.438
0.000
19


LNU4
25133.3
0.279
0.100
9
LNU122
25332.5
2.275
0.041
11


LNU4
25134.1
0.270
0.319
6
LNU122
25332.2
2.106
0.486
3


LNU4
25134.2
0.267
0.429
4
LNU125
25941.4
2.325
0.207
14


LNU40
24793.1
0.323
0.561
26
LNU125
25944.1
2.213
0.012
8


LNU40
24792.2
0.314
0.299
23
LNU125
25941.2
2.175
0.597
6


LNU40
24794.3
0.311
0.535
22
LNU125
25943.2
2.106
0.409
3


LNU40
24792.1
0.268
0.372
5
LNU125
25944.3
2.088
0.421
2


LNU40
24794.4
0.268
0.372
5
LNU178
14611.5
2.138
0.353
4


LNU46
14462.1
0.289
0.424
13
LNU178
14611.1
2.081
0.679
2


LNU46
14462.5
0.279
0.693
9
LNU234
25014.1
2.288
0.000
12


LNU48
24803.2
0.318
0.465
24
LNU234
25014.6
2.188
0.319
7


LNU48
24801.4
0.301
0.009
18
LNU234
25014.5
2.125
0.248
4


LNU48
24804.4
0.288
0.069
13
LNU236
25424.2
2.200
0.049
7


LNU48
24802.2
0.269
0.362
5
LNU236
25423.3
2.131
0.101
4


LNU48
24802.1
0.261
0.730
2
LNU24
24974.2
2.319
0.159
13


LNU63
24814.7
0.288
0.183
12
LNU24
24972.1
2.175
0.293
6


LNU7
25083.3
0.316
0.394
23
LNU25
14082.8
2.275
0.132
11


LNU7
25082.7
0.281
0.763
10
LNU271
25912.1
2.188
0.026
7


LNU7
25081.1
0.269
0.406
5
LNU271
25913.3
2.181
0.245
7


LNU8
25061.2
0.264
0.584
3
LNU271
25911.4
2.144
0.363
5


LNU94
24833.3
0.271
0.626
6
LNU278
25814.3
2.369
0.058
16


LNU94
24831.4
0.265
0.497
4
LNU278
25814.1
2.250
0.470
10


LNU96
25073.4
0.307
0.516
20
LNU278
25812.3
2.188
0.262
7


LNU96
25073.3
0.303
0.664
18
LNU278
25813.2
2.138
0.630
4


LNU96
25071.2
0.301
0.535
17
LNU43
14423.7
2.450
0.132
20


LNU96
25074.1
0.267
0.440
4
LNU43
14423.6
2.275
0.084
11


CONT.
—
0.243
—
0
LNU43
14421.1
2.106
0.646
3


LNU10
25123.6
0.283
0.160
17
LNU43
14422.8
2.100
0.750
3


LNU10
25123.5
0.265
0.218
9
LNU45
25053.4
2.356
0.000
15


LNU10
25123.1
0.246
0.773
1
LNU45
25052.12
2.244
0.010
10


LNU122
25332.1
0.277
0.167
14
LNU45
25052.11
2.106
0.409
3


LNU122
25332.5
0.266
0.002
9
LNU67
25823.5
2.331
0.000
14


LNU125
25941.4
0.285
0.000
17
LNU67
25824.5
2.313
0.000
13


LNU125
25944.1
0.275
0.000
13
LNU67
25821.5
2.288
0.009
12


LNU125
25943.2
0.264
0.003
9
LNU67
25824.3
2.194
0.587
7


LNU125
25941.2
0.261
0.562
7
LNU67
25821.4
2.169
0.025
6


LNU157
24982.8
0.259
0.114
7
LNU9
25003.1
2.219
0.171
8


LNU178
14611.5
0.265
0.295
9
LNU9
25001.7
2.156
0.243
5


LNU234
25014.4
0.267
0.253
10
LNU9
25001.3
2.138
0.189
4


LNU234
25014.5
0.262
0.005
8
LNU9
25001.2
2.113
0.668
3


LNU234
25014.1
0.258
0.552
6
CONT.
—
2.362
—
0


LNU234
25014.8
0.257
0.466
6
LNU10
25121.8
2.531
0.266
7


LNU234
25014.6
0.246
0.774
1
LNU10
25121.6
2.494
0.083
6


LNU236
25424.2
0.266
0.006
9
LNU10
25123.6
2.469
0.186
5


LNU236
25425.3
0.258
0.016
6
LNU157
24982.1
2.525
0.348
7


LNU236
25422.4
0.253
0.256
4
LNU157
24983.3
2.506
0.325
6


LNU24
24972.1
0.280
0.454
15
LNU157
24982.7
2.431
0.736
3


LNU24
24974.2
0.273
0.015
12
LNU168
24754.2
2.650
0.001
12


LNU24
24971.2
0.256
0.425
6
LNU168
24753.8
2.563
0.018
9


LNU25
14082.8
0.265
0.002
9
LNU168
24753.5
2.456
0.695
4


LNU267
25804.4
0.253
0.338
4
LNU168
24753.2
2.450
0.234
4


LNU271
25911.4
0.264
0.353
9
LNU173
25451.1
3.063
0.024
30


LNU271
25912.1
0.256
0.045
6
LNU173
25451.12
2.839
0.335
20


LNU271
25913.3
0.249
0.780
2
LNU173
25451.2
2.825
0.126
20


LNU278
25814.3
0.277
0.404
14
LNU173
25451.5
2.688
0.001
14


LNU278
25814.1
0.268
0.028
10
LNU173
25451.11
2.481
0.144
5


LNU278
25812.3
0.264
0.089
9
LNU178
14611.4
2.788
0.021
18


LNU278
25813.2
0.261
0.522
7
LNU178
14612.1
2.638
0.142
12


LNU43
14423.7
0.292
0.267
20
LNU178
14611.5
2.463
0.280
4


LNU43
14422.8
0.276
0.000
14
LNU178
14614.5
2.463
0.173
4


LNU43
14423.6
0.269
0.001
11
LNU178
14611.1
2.456
0.212
4


LNU45
25052.12
0.271
0.104
11
LNU184
25393.1
2.725
0.371
15


LNU45
25053.4
0.271
0.143
11
LNU184
25394.3
2.675
0.001
13


LNU67
25823.5
0.284
0.018
17
LNU184
25393.3
2.638
0.016
12


LNU67
25824.3
0.277
0.222
14
LNU184
25395.1
2.569
0.443
9


LNU67
25824.5
0.270
0.371
11
LNU20
24933.4
2.775
0.009
18


LNU67
25821.5
0.265
0.387
9
LNU20
24933.1
2.550
0.019
8


LNU67
25821.4
0.252
0.137
4
LNU20
24933.2
2.538
0.228
7


LNU9
25003.1
0.274
0.013
13
LNU20
24932.4
2.525
0.037
7


LNU9
25001.2
0.259
0.011
7
LNU20
24934.1
2.506
0.697
6


LNU9
25001.3
0.254
0.592
5
LNU230
25413.2
2.769
0.084
17


CONT.
—
0.240
—
0
LNU230
25415.1
2.744
0.227
16


LNU10
25123.6
0.256
0.224
7
LNU230
25412.2
2.656
0.001
12


LNU10
25121.8
0.253
0.717
5
LNU230
25412.1
2.625
0.003
11


LNU10
25121.6
0.248
0.530
3
LNU230
25413.1
2.563
0.014
9


LNU157
24982.4
0.265
0.764
10
LNU236
25425.4
2.725
0.000
15


LNU157
24982.1
0.262
0.085
9
LNU236
25422.4
2.650
0.409
12


LNU157
24982.8
0.261
0.293
9
LNU236
25424.2
2.638
0.142
12


LNU168
24754.2
0.259
0.131
8
LNU236
25425.3
2.625
0.322
11


LNU168
24751.2
0.253
0.333
5
LNU236
25423.3
2.519
0.188
7


LNU168
24753.2
0.250
0.647
4
LNU24
24974.2
2.775
0.000
18


LNU173
25451.1
0.295
0.044
23
LNU24
24971.2
2.581
0.447
9


LNU173
25451.2
0.274
0.032
14
LNU24
24971.4
2.550
0.066
8


LNU173
25451.12
0.259
0.643
8
LNU24
24971.3
2.506
0.588
6


LNU173
25451.5
0.251
0.396
4
LNU263
25791.3
2.738
0.376
16


LNU173
25451.11
0.250
0.411
4
LNU263
25794.8
2.675
0.466
13


LNU178
14611.4
0.275
0.011
14
LNU263
25792.2
2.606
0.636
10


LNU178
14612.1
0.256
0.279
6
LNU263
25794.6
2.556
0.531
8


LNU178
14611.5
0.253
0.427
5
LNU263
25794.3
2.456
0.200
4


LNU178
14614.5
0.251
0.584
5
LNU276
25431.1
2.513
0.418
6


LNU184
25393.1
0.276
0.409
15
LNU276
25433.1
2.413
0.714
2


LNU184
25395.1
0.275
0.074
14
LNU279
25481.4
2.644
0.009
12


LNU184
25393.2
0.259
0.724
8
LNU279
25481.3
2.563
0.014
9


LNU184
25393.3
0.258
0.155
7
LNU279
25484.3
2.425
0.386
3


LNU184
25394.3
0.249
0.468
4
LNU279
25481.5
2.406
0.534
2


LNU20
24933.2
0.278
0.604
16
LNU279
25481.2
2.381
0.783
1


LNU20
24933.4
0.275
0.053
14
LNU36
25562.9
2.706
0.051
15


LNU20
24934.1
0.261
0.418
9
LNU36
25561.2
2.675
0.544
13


LNU20
24933.1
0.257
0.184
7
LNU36
25562.4
2.656
0.109
12


LNU230
25415.1
0.277
0.258
15
LNU36
25562.3
2.513
0.514
6


LNU230
25413.2
0.264
0.084
10
LNU36
25562.7
2.456
0.335
4


LNU230
25413.1
0.258
0.159
7
LNU53
25674.1
2.669
0.203
13


LNU230
25412.1
0.253
0.282
5
LNU53
25674.6
2.563
0.137
9


LNU230
25412.2
0.248
0.538
3
LNU53
25674.5
2.506
0.217
6


LNU236
25422.4
0.281
0.397
17
LNU53
25674.2
2.488
0.576
5


LNU236
25425.4
0.273
0.047
13
LNU56
24694.1
2.694
0.120
14


LNU236
25424.2
0.261
0.362
9
LNU56
24693.2
2.656
0.020
12


LNU236
25425.3
0.257
0.172
7
LNU56
24694.2
2.625
0.192
11


LNU24
24971.2
0.269
0.225
12
LNU56
24693.1
2.531
0.355
7


LNU24
24974.2
0.260
0.139
8
LNU73
25751.8
2.463
0.231
4


LNU24
24971.4
0.259
0.148
8
LNU73
25751.1
2.450
0.446
4


LNU24
24971.3
0.251
0.381
4
LNU9
25001.3
2.725
0.000
15


LNU263
25794.8
0.306
0.504
27
LNU9
25001.7
2.719
0.136
15


LNU263
25791.3
0.271
0.586
13
LNU9
25001.2
2.600
0.431
10


LNU263
25792.2
0.270
0.759
12
LNU9
25003.1
2.475
0.585
5


LNU263
25794.6
0.267
0.369
11
CONT.
—
2.036
—
0


LNU263
25794.3
0.254
0.535
6
LNU173
25451.5
2.394
0.002
18


LNU276
25431.1
0.290
0.201
21
LNU173
25451.2
2.369
0.294
16


LNU276
25433.2
0.248
0.544
3
LNU173
25451.1
2.189
0.154
8


LNU276
25433.1
0.246
0.665
2
LNU181
25771.2
2.338
0.095
15


LNU279
25481.4
0.268
0.417
11
LNU181
25771.5
2.275
0.509
12


LNU279
25481.3
0.265
0.201
10
LNU181
25771.6
2.219
0.332
9


LNU279
25481.5
0.250
0.431
4
LNU181
25771.8
2.150
0.206
6


LNU279
25481.2
0.246
0.759
3
LNU184
25394.1
2.338
0.521
15


LNU36
25562.4
0.269
0.404
12
LNU184
25393.2
2.238
0.279
10


LNU36
25561.2
0.269
0.305
12
LNU184
25393.3
2.220
0.530
9


LNU36
25562.9
0.252
0.406
5
LNU184
25394.3
2.106
0.663
3


LNU36
25562.3
0.249
0.479
4
LNU224
25872.3
2.344
0.144
15


LNU53
25674.1
0.260
0.355
8
LNU224
25874.1
2.269
0.018
11


LNU53
25674.2
0.246
0.679
2
LNU224
25874.4
2.250
0.016
11


LNU53
25674.6
0.245
0.697
2
LNU224
25872.2
2.188
0.166
7


LNU56
24694.1
0.261
0.109
9
LNU246
25744.2
2.331
0.080
15


LNU56
24694.2
0.254
0.445
6
LNU246
25743.1
2.287
0.001
12


LNU56
24693.1
0.246
0.711
2
LNU246
25743.2
2.188
0.027
7


LNU73
25751.1
0.259
0.621
8
LNU246
25744.3
2.106
0.663
3


LNU9
25001.7
0.264
0.193
10
LNU250
25592.1
2.319
0.535
14


LNU9
25001.2
0.264
0.063
10
LNU250
25592.2
2.319
0.023
14


LNU9
25003.1
0.256
0.343
7
LNU250
25591.1
2.113
0.244
4


LNU9
25001.3
0.252
0.359
5
LNU260
26403.1
2.194
0.513
8


CONT.
—
0.178
—
0
LNU260
26404.7
2.163
0.079
6


LNU131
14005.5
0.208
0.034
17
LNU260
26404.1
2.144
0.325
5


LNU131
14005.2
0.204
0.069
14
LNU260
26403.2
2.081
0.774
2


LNU135
26204.2
0.206
0.109
16
LNU276
25433.3
2.356
0.000
16


LNU135
26203.6
0.204
0.230
14
CONT.
—
1.044
—
0


LNU135
26203.4
0.196
0.793
10
LNU136
14515.1
1.313
0.374
26


LNU135
26203.1
0.188
0.712
5
LNU136
14515.5
1.263
0.005
21


LNU173
25451.2
0.239
0.030
34
LNU136
14511.10
1.150
0.466
10


LNU173
25451.5
0.226
0.009
27
LNU142
27541.1
1.344
0.125
29


LNU173
25451.11
0.205
0.421
15
LNU142
27541.2
1.219
0.389
17


LNU173
25451.1
0.193
0.703
8
LNU149
26175.1
1.225
0.480
17


LNU181
25771.6
0.224
0.281
25
LNU149
26175.3
1.131
0.404
8


LNU181
25771.2
0.217
0.010
22
LNU149
26175.7
1.131
0.337
8


LNU181
25771.8
0.209
0.086
17
LNU15
14123.13
1.244
0.123
19


LNU181
25771.5
0.204
0.423
14
LNU15
14122.8
1.163
0.061
11


LNU181
25771.11
0.200
0.105
12
LNU15
14122.9
1.156
0.304
11


LNU184
25394.1
0.236
0.114
32
LNU15
14123.11
1.156
0.112
11


LNU184
25393.2
0.229
0.001
28
LNU185
26474.2
1.206
0.066
16


LNU184
25395.1
0.215
0.088
20
LNU185
26475.1
1.144
0.102
10


LNU184
25393.3
0.207
0.186
16
LNU185
26474.1
1.131
0.714
8


LNU184
25394.3
0.203
0.287
14
LNU212
25834.1
1.356
0.269
30


LNU184
25393.1
0.196
0.175
10
LNU212
25832.1
1.233
0.246
18


LNU224
25874.1
0.223
0.003
25
LNU212
25834.4
1.195
0.255
14


LNU224
25872.3
0.214
0.418
20
LNU212
25833.2
1.150
0.605
10


LNU224
25874.4
0.214
0.011
20
LNU212
25834.5
1.138
0.552
9


LNU224
25871.3
0.206
0.219
15
LNU216
25985.4
1.369
0.232
31


LNU224
25872.2
0.194
0.465
9
LNU216
25982.1
1.363
0.223
31


LNU246
25744.3
0.237
0.000
33
LNU228
26222.1
1.269
0.306
22


LNU246
25744.2
0.231
0.030
30
LNU228
26222.4
1.169
0.049
12


LNU246
25743.1
0.223
0.003
25
LNU228
26224.6
1.119
0.466
7


LNU246
25743.2
0.218
0.028
22
LNU229
26112.3
1.094
0.659
5


LNU246
25744.4
0.212
0.097
19
LNU277
25842.3
1.150
0.545
10


LNU250
25591.1
0.221
0.016
24
LNU280
26162.1
1.394
0.038
34


LNU250
25592.2
0.216
0.013
21
LNU280
26162.7
1.166
0.378
12


LNU250
25592.1
0.213
0.159
19
LNU280
26164.4
1.144
0.211
10


LNU250
25591.3
0.186
0.684
4
LNU55
26015.1
1.356
0.006
30


LNU260
26404.1
0.215
0.032
20
LNU55
26015.3
1.219
0.011
17


LNU260
26404.7
0.211
0.027
18
LNU81
26034.3
1.194
0.140
14


LNU260
26403.1
0.204
0.119
15
LNU81
26031.2
1.125
0.172
8


LNU260
26403.2
0.195
0.312
9
CONT.
—
1.494
—
0


LNU260
26404.8
0.185
0.681
4
LNU119
26141.1
1.806
0.082
21


LNU276
25433.3
0.218
0.006
22
LNU119
26142.8
1.744
0.472
17


LNU276
25433.6
0.213
0.066
19
LNU119
26144.1
1.613
0.619
8


LNU276
25433.5
0.188
0.475
5
LNU119
26144.2
1.606
0.157
8


LNU276
25431.1
0.185
0.614
4
LNU119
26142.5
1.575
0.705
5


LNU279
25484.3
0.216
0.017
21
LNU130
24913.5
1.856
0.340
24


LNU279
25481.5
0.215
0.046
20
LNU130
24911.7
1.813
0.030
21


LNU279
25481.3
0.199
0.223
11
LNU130
24913.6
1.700
0.219
14


LNU279
25481.4
0.184
0.646
3
LNU130
24912.7
1.675
0.371
12


LNU3
26124.3
0.218
0.073
22
LNU136
14514.8
1.906
0.106
28


LNU3
26122.2
0.205
0.050
15
LNU136
14511.10
1.731
0.165
16


LNU3
26123.5
0.203
0.162
14
LNU136
14515.1
1.725
0.192
15


LNU3
26124.1
0.193
0.528
8
LNU142
27546.2
2.000
0.300
34


LNU33
25552.2
0.223
0.023
25
LNU142
27541.2
1.919
0.001
28


LNU33
25551.1
0.186
0.589
4
LNU142
27545.1
1.844
0.100
23


CONT.
—
0.079
—
0
LNU142
27546.1
1.769
0.266
18


LNU119
26142.5
0.085
0.725
8
LNU142
27541.1
1.625
0.157
9


LNU130
24914.5
0.085
0.186
8
LNU149
26174.7
1.756
0.306
18


LNU130
24913.6
0.084
0.792
7
LNU149
26174.6
1.659
0.375
11


LNU130
24912.7
0.083
0.422
5
LNU149
26175.3
1.638
0.321
10


LNU136
14515.5
0.108
0.000
38
LNU149
26174.8
1.538
0.272
3


LNU136
14515.1
0.108
0.377
37
LNU15
14122.8
1.894
0.001
27


LNU136
14511.10
0.092
0.107
17
LNU15
14123.11
1.713
0.281
15


LNU142
27541.1
0.111
0.000
41
LNU15
14124.12
1.625
0.510
9


LNU142
27541.2
0.093
0.086
18
LNU185
26474.1
1.806
0.283
21


LNU142
27545.1
0.088
0.528
12
LNU185
26473.1
1.750
0.206
17


LNU142
27546.2
0.085
0.411
8
LNU185
26475.1
1.694
0.102
13


LNU149
26175.1
0.104
0.357
33
LNU185
26474.2
1.644
0.376
10


LNU149
26175.3
0.095
0.463
21
LNU212
25834.4
2.106
0.000
41


LNU149
26175.7
0.084
0.421
7
LNU212
25834.5
2.094
0.000
40


LNU15
14123.11
0.104
0.000
33
LNU212
25834.1
2.019
0.079
35


LNU15
14123.13
0.102
0.304
30
LNU212
25833.2
1.944
0.118
30


LNU15
14122.9
0.100
0.016
27
LNU212
25833.1
1.600
0.705
7


LNU15
14122.8
0.099
0.033
26
LNU216
25984.1
2.238
0.043
50


LNU185
26474.2
0.099
0.001
26
LNU216
25982.2
2.169
0.073
45


LNU185
26473.1
0.088
0.651
12
LNU216
25985.4
2.113
0.239
41


LNU185
26474.1
0.088
0.084
11
LNU216
25984.6
2.094
0.000
40


LNU212
25832.1
0.115
0.117
46
LNU216
25982.1
1.850
0.000
24


LNU212
25834.4
0.107
0.172
36
LNU228
26222.1
2.688
0.000
80


LNU212
25834.1
0.101
0.043
29
LNU228
26224.7
2.381
0.040
59


LNU212
25833.2
0.094
0.565
19
LNU228
26222.4
2.301
0.066
54


LNU212
25834.5
0.094
0.425
19
LNU228
26225.2
2.256
0.000
51


LNU216
25982.1
0.114
0.219
45
LNU228
26224.6
2.081
0.244
39


LNU216
25985.4
0.108
0.055
37
LNU229
26111.5
2.194
0.002
47


LNU228
26222.1
0.103
0.001
30
LNU229
26112.4
2.175
0.000
46


LNU228
26222.4
0.098
0.002
24
LNU229
26111.7
2.163
0.000
45


LNU228
26224.6
0.094
0.238
19
LNU229
26112.3
2.038
0.243
36


LNU228
26224.7
0.083
0.422
5
LNU229
26114.1
1.931
0.185
29


LNU229
26112.3
0.096
0.466
22
LNU241
26232.4
2.163
0.000
45


LNU253
26241.1
0.083
0.514
6
LNU241
26234.1
2.163
0.018
45


LNU277
25842.3
0.099
0.370
26
LNU241
26232.1
1.881
0.209
26


LNU277
25844.4
0.089
0.166
14
LNU241
26233.2
1.881
0.383
26


LNU277
25844.3
0.082
0.765
4
LNU241
26233.3
1.744
0.415
17


LNU280
26162.1
0.114
0.002
45
LNU253
26242.1
2.175
0.000
46


LNU280
26162.7
0.096
0.081
23
LNU253
26241.1
2.100
0.000
41


LNU280
26164.4
0.092
0.053
17
LNU253
26243.3
2.069
0.169
38


LNU280
26164.2
0.083
0.374
6
LNU253
26245.1
1.794
0.707
20


LNU55
26015.1
0.097
0.100
23
LNU253
26244.2
1.781
0.165
19


LNU55
26015.3
0.094
0.006
20
LNU274
26264.2
2.331
0.030
56


LNU81
26031.2
0.099
0.077
26
LNU274
26265.1
2.238
0.147
50


LNU81
26034.3
0.092
0.311
17
LNU274
26262.2
2.225
0.087
49


LNU81
26031.10
0.088
0.570
11
LNU274
26263.2
2.225
0.113
49


CONT.
—
0.150
—
0
LNU274
26261.3
2.113
0.008
41


LNU119
26142.8
0.160
0.721
7
LNU277
25844.3
2.094
0.000
40


LNU119
26141.1
0.157
0.673
5
LNU277
25842.3
2.013
0.028
35


LNU130
24913.5
0.168
0.234
12
LNU277
25844.4
1.988
0.136
33


LNU130
24913.6
0.159
0.303
6
LNU277
25841.3
1.881
0.230
26


LNU130
24911.7
0.156
0.412
4
LNU277
25845.1
1.625
0.589
9


LNU136
14514.8
0.193
0.421
28
LNU280
26162.1
2.469
0.006
65


LNU136
14511.10
0.164
0.110
10
LNU280
26164.4
2.263
0.001
51


LNU136
14515.1
0.163
0.361
8
LNU280
26162.7
2.094
0.368
40


LNU142
27546.2
0.176
0.494
17
LNU280
26164.3
1.888
0.255
26


LNU142
27541.2
0.171
0.037
14
LNU280
26164.2
1.700
0.487
14


LNU142
27545.1
0.171
0.032
14
LNU55
26013.9
2.219
0.067
49


LNU142
27546.1
0.153
0.768
2
LNU55
26013.3
2.181
0.000
46


LNU15
14123.11
0.171
0.491
14
LNU55
26015.1
1.881
0.001
26


LNU15
14122.8
0.166
0.087
11
LNU81
26034.2
1.956
0.572
31


LNU15
14124.12
0.160
0.721
7
LNU81
26031.10
1.781
0.671
19


LNU185
26473.1
0.174
0.019
16
LNU81
26034.3
1.581
0.514
6


LNU185
26474.1
0.170
0.236
13







LNU185
26475.1
0.157
0.757
5







LNU212
25834.5
0.186
0.004
24







LNU212
25833.2
0.178
0.250
18







LNU212
25834.1
0.175
0.016
17







LNU212
25834.4
0.175
0.015
17







LNU216
25982.1
0.194
0.348
30







LNU216
25982.2
0.192
0.002
28







LNU216
25985.4
0.179
0.237
19







LNU216
25984.1
0.178
0.146
18







LNU216
25984.6
0.171
0.204
14







LNU228
26222.1
0.251
0.000
67







LNU228
26222.4
0.206
0.108
37







LNU228
26224.7
0.199
0.024
33







LNU228
26225.2
0.196
0.081
31







LNU228
26224.6
0.171
0.365
14







LNU229
26111.7
0.190
0.006
27







LNU229
26111.5
0.184
0.056
23







LNU229
26112.4
0.184
0.007
23







LNU229
26114.1
0.167
0.397
11







LNU229
26112.3
0.163
0.123
9







LNU241
26232.4
0.190
0.002
27







LNU241
26234.1
0.183
0.008
22







LNU241
26232.1
0.171
0.129
14







LNU241
26233.2
0.169
0.467
13







LNU241
26233.3
0.153
0.719
2







LNU253
26242.1
0.188
0.003
25







LNU253
26241.1
0.185
0.005
23







LNU253
26243.3
0.171
0.057
14







LNU253
26244.2
0.156
0.523
4







LNU274
26264.2
0.211
0.000
40







LNU274
26263.2
0.193
0.025
28







LNU274
26262.2
0.190
0.269
27







LNU274
26261.3
0.188
0.003
25







LNU274
26265.1
0.179
0.465
20







LNU277
25844.3
0.198
0.003
32







LNU277
25841.3
0.181
0.434
20







LNU277
25844.4
0.171
0.204
14







LNU277
25842.3
0.160
0.348
7







LNU280
26162.1
0.216
0.134
44







LNU280
26164.4
0.191
0.002
28







LNU280
26162.7
0.169
0.673
13







LNU280
26164.3
0.169
0.491
13







LNU55
26013.3
0.194
0.042
29







LNU55
26013.9
0.190
0.227
27







LNU55
26015.1
0.168
0.057
12







LNU81
26034.2
0.174
0.732
16





Table 82.


“CONT.”—Control;


“Ave.”—Average;


“% Incr.” = % increment.













TABLE 83







Genes showing improved plant biomass production


at standard nitrogen growth conditions













Rosette

Rosette Area

Plot Coverage



Diameter [cm]

[cm2]

[%]





















Gene


P-
%
Gene


P-
%
Gene


P-
%


Name
Event #
Ave.
Value
incr.
Name
Event #
Ave.
Value
incr.
Name
Event #
Ave.
Value
incr.
























CONT.
—
3.65
—
0
CONT.
—
4.53
—
0
CONT.
—
35.85
—
0


LNU100
14471.4
4.39
0.208
20
LNU100
14471.4
6.19
0.343
37
LNU100
14471.4
49.56
0.334
38


LNU100
14474.3
4.22
0.051
16
LNU100
14474.3
5.82
0.061
28
LNU100
14474.3
46.53
0.052
30


LNU100
14472.2
4.16
0.159
14
LNU100
14473.3
5.78
0.148
27
LNU100
14473.3
46.21
0.136
29


LNU100
14473.3
4.07
0.105
12
LNU100
14472.2
5.61
0.199
24
LNU100
14472.2
44.86
0.184
25


LNU104
25032.2
4.52
0.083
24
LNU104
25032.2
7.12
0.090
57
LNU104
25032.2
56.93
0.085
59


LNU104
25033.1
4.46
0.131
22
LNU104
25033.1
6.47
0.063
43
LNU104
25033.1
51.77
0.056
44


LNU104
25032.1
4.32
0.074
18
LNU104
25032.1
6.29
0.129
39
LNU104
25032.1
50.31
0.121
40


LNU104
25033.3
4.26
0.023
17
LNU104
25033.3
6.07
0.000
34
LNU104
25033.3
48.53
0.000
35


LNU104
25034.1
3.84
0.276
5
LNU104
25034.1
5.06
0.235
12
LNU104
25034.1
40.49
0.204
13


LNU106
14482.3
4.27
0.401
17
LNU106
14482.3
5.94
0.358
31
LNU106
14482.3
47.55
0.347
33


LNU106
14483.5
4.19
0.000
15
LNU106
14483.5
5.72
0.063
26
LNU106
14483.5
45.73
0.054
28


LNU106
14483.2
4.11
0.218
13
LNU106
14483.2
5.69
0.340
26
LNU106
14483.2
45.51
0.326
27


LNU106
14481.1
3.77
0.065
3
LNU114
25041.1
6.77
0.000
49
LNU114
25041.1
54.13
0.000
51


LNU114
25041.1
4.54
0.000
24
LNU114
25042.1
5.62
0.144
24
LNU114
25042.1
44.92
0.130
25


LNU114
25042.1
4.13
0.084
13
LNU114
25041.2
5.18
0.018
14
LNU114
25041.2
41.40
0.013
15


LNU114
25041.2
3.96
0.112
9
LNU155
14525.6
5.63
0.118
24
LNU155
14525.6
45.03
0.105
26


LNU155
14525.6
4.16
0.115
14
LNU155
14525.1
5.43
0.517
20
LNU155
14525.1
43.44
0.499
21


LNU155
14525.1
3.97
0.517
9
LNU213
24653.2
6.87
0.000
51
LNU213
24653.2
54.92
0.000
53


LNU213
24653.2
4.55
0.000
25
LNU213
24652.4
6.04
0.458
33
LNU213
24652.4
48.29
0.448
35


LNU213
24652.4
4.25
0.477
16
LNU213
24653.1
5.21
0.001
15
LNU213
24653.1
41.64
0.001
16


LNU213
24653.1
3.88
0.004
6
LNU218
24781.1
6.41
0.167
41
LNU218
24781.1
51.27
0.159
43


LNU213
24654.4
3.79
0.713
4
LNU218
24781.7
6.10
0.215
35
LNU218
24781.7
48.78
0.206
36


LNU218
24781.1
4.43
0.199
21
LNU218
24781.2
5.24
0.306
16
LNU218
24781.2
41.96
0.282
17


LNU218
24781.7
4.30
0.190
18
LNU23
25163.5
6.90
0.010
52
LNU23
25163.5
55.20
0.008
54


LNU218
24781.2
3.98
0.000
9
LNU23
25163.6
5.17
0.442
14
LNU23
25163.6
41.39
0.416
15


LNU23
25163.5
4.47
0.000
23
LNU28
25171.2
6.00
0.122
32
LNU28
25171.2
47.99
0.112
34


LNU23
25163.6
3.87
0.342
6
LNU28
25174.3
5.31
0.173
17
LNU28
25174.3
42.47
0.154
18


LNU23
25163.4
3.72
0.764
2
LNU28
25171.1
5.15
0.003
14
LNU28
25171.1
38.56
0.403
8


LNU28
25171.2
4.17
0.193
14
LNU28
25171.4
4.82
0.522
6
LNU28
25171.4
38.54
0.464
8


LNU28
25171.1
4.04
0.215
11
LNU28
25174.5
4.72
0.769
4
LNU28
25174.5
37.74
0.713
5


LNU28
25174.3
3.93
0.086
8
LNU4
25134.1
6.45
0.020
42
LNU4
25134.1
51.60
0.015
44


LNU28
25171.4
3.82
0.422
5
LNU4
25133.3
5.78
0.001
28
LNU4
25133.3
46.23
0.001
29


LNU28
25174.5
3.74
0.715
3
LNU4
25134.3
5.42
0.390
20
LNU4
25134.3
43.35
0.372
21


LNU4
25134.1
4.33
0.052
19
LNU4
25134.2
5.00
0.352
10
LNU4
25134.2
40.03
0.315
12


LNU4
25133.3
4.26
0.004
17
LNU4
25131.1
4.82
0.730
6
LNU4
25131.1
38.56
0.689
8


LNU4
25134.3
3.95
0.423
8
LNU40
24794.3
6.09
0.075
34
LNU40
24794.3
48.69
0.066
36


LNU4
25134.2
3.82
0.388
5
LNU40
24794.4
5.79
0.278
28
LNU40
24794.4
46.29
0.265
29


LNU4
25131.1
3.78
0.702
4
LNU40
24792.1
4.96
0.024
9
LNU40
24792.1
39.69
0.019
11


LNU40
24794.3
4.22
0.021
16
LNU40
24792.2
4.93
0.568
9
LNU40
24792.2
39.44
0.528
10


LNU40
24794.4
4.12
0.136
13
LNU46
14464.4
6.71
0.233
48
LNU46
14464.4
53.69
0.227
50


LNU40
24792.1
3.78
0.048
4
LNU46
14463.1
6.56
0.339
45
LNU46
14463.1
52.50
0.331
46


LNU46
14463.1
4.49
0.310
23
LNU46
14462.5
6.24
0.000
38
LNU46
14462.5
49.91
0.000
39


LNU46
14464.4
4.38
0.219
20
LNU46
14462.1
5.39
0.339
19
LNU46
14462.1
40.68
0.601
13


LNU46
14462.5
4.34
0.000
19
LNU48
24801.4
7.25
0.000
60
LNU48
24801.4
58.02
0.000
62


LNU46
14462.1
3.98
0.418
9
LNU48
24802.2
6.41
0.050
41
LNU48
24802.2
51.27
0.044
43


LNU48
24801.4
4.61
0.000
26
LNU48
24802.1
6.01
0.051
33
LNU48
24802.1
48.11
0.044
34


LNU48
24802.1
4.41
0.039
21
LNU63
24814.2
6.06
0.418
34
LNU63
24814.2
48.51
0.408
35


LNU48
24802.2
4.33
0.026
19
LNU63
24812.2
5.31
0.254
17
LNU63
24812.2
42.49
0.233
19


LNU63
24814.2
4.25
0.454
17
LNU63
24814.3
5.22
0.391
15
LNU63
24814.3
41.77
0.367
17


LNU63
24814.3
3.97
0.477
9
LNU63
24811.2
4.78
0.772
6
LNU7
25081.1
45.92
0.015
28


LNU63
24812.2
3.93
0.357
8
LNU7
25081.1
5.74
0.019
27
LNU7
25083.1
44.18
0.044
23


LNU7
25083.1
4.12
0.000
13
LNU7
25083.1
5.52
0.053
22
LNU7
25083.3
40.05
0.530
12


LNU7
25081.1
4.09
0.000
12
LNU7
25083.3
5.01
0.563
10
LNU7
25082.2
37.72
0.193
5


LNU7
25083.3
3.74
0.704
3
LNU7
25082.2
4.71
0.267
4
LNU8
25063.6
46.26
0.036
29


LNU7
25082.2
3.73
0.217
2
LNU8
25063.6
5.78
0.043
28
LNU8
25062.2
42.10
0.315
17


LNU8
25063.6
4.19
0.064
15
LNU8
25062.2
5.26
0.338
16
LNU8
25061.2
38.84
0.393
8


LNU8
25062.2
3.85
0.582
6
LNU8
25061.2
4.86
0.447
7
LNU8
25063.1
38.51
0.289
7


LNU8
25061.2
3.84
0.299
5
LNU8
25063.1
4.81
0.351
6
LNU94
24833.3
47.02
0.289
31


LNU8
25063.1
3.77
0.071
3
LNU94
24833.3
5.88
0.301
30
LNU94
24831.4
45.54
0.000
27


LNU94
24833.3
4.12
0.202
13
LNU94
24831.4
5.69
0.000
26
LNU94
24834.4
42.80
0.036
19


LNU94
24831.4
4.00
0.001
10
LNU94
24834.4
5.35
0.046
18
LNU94
24833.1
39.16
0.691
9


LNU94
24834.4
3.99
0.101
9
LNU94
24833.1
4.89
0.724
8
LNU96
25073.3
42.52
0.451
19


LNU94
24833.1
3.77
0.724
3
LNU96
25073.3
5.31
0.472
17
LNU96
25071.3
41.84
0.050
17


LNU96
25073.3
4.05
0.372
11
LNU96
25071.3
5.23
0.063
15
CONT.
—
56.10
—
0


LNU96
25071.3
3.92
0.111
7
CONT.
—
7.01
—
0
LNU148
25685.6
59.24
0.687
6


LNU96
25073.4
3.76
0.755
3
LNU148
25685.6
7.41
0.687
6
LNU5
14042.7
61.42
0.016
9


CONT.
—
4.37
—
0
LNU5
14042.7
7.68
0.016
9
LNU72
24962.3
60.29
0.584
7


LNU5
14042.7
4.56
0.028
4
LNU72
24962.3
7.54
0.584
7
LNU98
25763.2
58.27
0.297
4


LNU72
24962.3
4.58
0.611
5
LNU98
25763.2
7.28
0.297
4
CONT.
—
49.17
—
0


LNU98
25763.2
4.53
0.195
4
CONT.
—
6.21
—
0
LNU113
25631.1
57.73
0.070
17


CONT.
—
4.09
—
0
LNU113
25631.1
7.22
0.082
16
LNU113
25631.3
56.54
0.380
15


LNU113
25631.1
4.59
0.128
12
LNU113
25631.3
7.07
0.406
14
LNU124
14504.5
56.31
0.308
15


LNU113
25631.3
4.47
0.296
9
LNU124
14504.5
7.04
0.335
13
LNU148
25685.1
56.54
0.036
15


LNU124
14504.5
4.52
0.176
11
LNU148
25685.1
7.07
0.044
14
LNU148
25685.2
54.69
0.758
11


LNU132
14102.9
4.33
0.705
6
LNU148
25685.2
6.84
0.778
10
LNU37
14064.7
63.16
0.018
28


LNU148
25685.1
4.40
0.068
7
LNU37
14064.7
7.89
0.021
27
LNU37
14064.6
54.98
0.659
12


LNU287
24674.3
4.30
0.654
5
LNU37
14064.6
6.87
0.684
11
LNU5
14043.9
63.55
0.068
29


LNU37
14064.7
4.77
0.034
16
LNU5
14043.9
7.94
0.075
28
LNU5
14043.7
51.12
0.681
4


LNU37
14064.6
4.55
0.461
11
LNU5
14043.7
6.39
0.757
3
LNU65
24702.3
52.18
0.687
6


LNU5
14043.9
4.52
0.129
11
LNU65
24702.3
6.52
0.734
5
LNU68
14034.13
64.85
0.369
32


LNU65
24703.6
4.32
0.434
6
LNU68
14034.13
8.11
0.379
31
LNU71
25852.5
62.11
0.105
26


LNU65
24702.3
4.28
0.759
5
LNU71
25852.5
7.76
0.114
25
LNU71
25853.1
61.33
0.002
25


LNU68
14034.13
4.73
0.410
16
LNU71
25853.1
7.67
0.003
23
LNU71
25851.4
55.90
0.258
14


LNU71
25853.1
4.76
0.000
16
LNU71
25851.4
6.99
0.287
13
LNU72
24963.7
55.78
0.372
13


LNU71
25852.5
4.67
0.198
14
LNU72
24963.7
6.97
0.402
12
LNU72
24963.8
50.14
0.789
2


LNU71
25851.4
4.57
0.245
12
LNU74
25443.3
7.10
0.538
14
LNU74
25443.3
56.78
0.515
15


LNU72
24963.7
4.47
0.518
9
LNU82
24823.1
6.66
0.450
7
LNU82
24823.1
53.30
0.399
8


LNU72
24963.8
4.16
0.749
2
LNU84
25621.2
6.58
0.562
6
LNU84
25621.2
52.68
0.506
7


LNU74
25443.3
4.58
0.398
12
LNU84
25621.4
6.55
0.392
5
LNU84
25621.4
52.36
0.324
6


LNU74
25444.1
4.20
0.485
3
CONT.
—
7.69
—
0
CONT.
—
60.12
—
0


LNU82
24823.1
4.32
0.164
6
LNU117
25931.4
10.65
0.009
38
LNU117
25931.4
85.18
0.007
42


LNU84
25621.2
4.39
0.279
7
LNU117
25933.3
10.29
0.003
34
LNU117
25933.3
82.33
0.005
37


LNU84
25621.4
4.25
0.299
4
LNU117
25931.1
8.36
0.259
9
LNU117
25931.1
66.92
0.211
11


LNU87
24742.1
4.29
0.647
5
LNU117
25932.4
8.00
0.520
4
LNU117
25932.4
63.99
0.400
6


CONT.
—
4.56
—
0
LNU117
25931.2
7.88
0.794
2
LNU117
25931.2
63.01
0.633
5


LNU117
25931.4
5.36
0.121
18
LNU122
25333.2
10.01
0.004
30
LNU122
25333.2
80.11
0.007
33


LNU117
25933.3
5.32
0.013
17
LNU122
25333.1
9.61
0.013
25
LNU122
25333.1
76.90
0.015
28


LNU117
25931.1
4.81
0.421
5
LNU122
25332.2
9.57
0.061
24
LNU122
25332.2
76.60
0.047
27


LNU117
25932.4
4.74
0.194
4
LNU122
25332.5
9.29
0.340
21
LNU122
25332.5
74.32
0.298
24


LNU117
25931.2
4.72
0.564
3
LNU122
25332.1
9.00
0.309
17
LNU122
25332.1
72.00
0.260
20


LNU122
25333.2
5.09
0.046
12
LNU125
25941.4
9.65
0.009
25
LNU125
25941.4
77.19
0.012
28


LNU122
25332.2
5.06
0.023
11
LNU125
25944.3
8.58
0.410
12
LNU125
25944.3
68.65
0.342
14


LNU122
25332.1
5.01
0.165
10
LNU125
25941.2
8.07
0.588
5
LNU125
25941.2
64.54
0.464
7


LNU122
25333.1
4.99
0.040
9
LNU138
14074.6
9.67
0.108
26
LNU138
14074.6
77.36
0.083
29


LNU122
25332.5
4.93
0.447
8
LNU138
14074.5
9.38
0.024
22
LNU138
14074.5
75.02
0.025
25


LNU125
25941.4
5.22
0.062
14
LNU180
24722.2
8.14
0.371
6
LNU180
24722.2
65.08
0.298
8


LNU125
25944.3
4.83
0.100
6
LNU220
25405.1
8.16
0.413
6
LNU180
24724.1
62.46
0.776
4


LNU138
14074.5
5.17
0.005
13
LNU220
25405.6
8.07
0.724
5
LNU220
25405.1
65.27
0.324
9


LNU138
14074.6
5.12
0.008
12
LNU230
25413.1
9.23
0.467
20
LNU220
25405.6
64.54
0.617
7


LNU180
24724.1
4.69
0.503
3
LNU230
25412.2
9.22
0.477
20
LNU220
25405.3
63.52
0.713
6


LNU180
24722.2
4.68
0.399
3
LNU230
25413.2
8.92
0.659
16
LNU220
25405.2
62.55
0.703
4


LNU180
24723.1
4.64
0.532
2
LNU25
14083.7
8.47
0.614
10
LNU230
25413.1
73.84
0.427
23


LNU220
25405.6
4.82
0.397
6
LNU25
14083.1
8.41
0.174
9
LNU230
25412.2
73.79
0.437
23


LNU230
25413.1
5.04
0.391
11
LNU25
14082.8
8.39
0.185
9
LNU230
25413.2
71.33
0.619
19


LNU230
25412.2
4.99
0.456
9
LNU25
14082.9
8.01
0.505
4
LNU25
14083.7
67.72
0.546
13


LNU230
25413.2
4.89
0.710
7
LNU254
25782.4
8.05
0.580
5
LNU25
14083.1
67.24
0.158
12


LNU230
25412.1
4.66
0.755
2
LNU254
25781.3
7.96
0.605
3
LNU25
14082.8
67.09
0.166
12


LNU25
14082.8
4.86
0.066
7
LNU263
25791.3
9.99
0.294
30
LNU25
14082.9
64.08
0.390
7


LNU25
14082.9
4.79
0.207
5
LNU263
25794.8
9.48
0.103
23
LNU254
25782.4
64.44
0.454
7


LNU25
14083.1
4.77
0.146
5
LNU267
25804.3
8.13
0.675
6
LNU254
25781.3
63.70
0.462
6


LNU25
14083.7
4.70
0.756
3
LNU271
25912.1
8.11
0.778
5
LNU263
25791.3
79.91
0.265
33


LNU254
25781.3
4.71
0.283
3
LNU278
25814.3
8.80
0.498
14
LNU263
25794.8
75.84
0.080
26


LNU254
25782.5
4.70
0.435
3
LNU278
25814.1
8.01
0.539
4
LNU263
25794.6
61.72
0.759
3


LNU254
25782.4
4.68
0.777
3
LNU278
25812.3
7.95
0.661
3
LNU267
25804.3
65.00
0.570
8


LNU263
25791.3
5.30
0.257
16
LNU36
25562.3
8.31
0.386
8
LNU271
25913.3
63.74
0.687
6


LNU263
25794.8
5.21
0.133
14
LNU43
14422.8
8.81
0.099
14
LNU278
25814.3
70.43
0.444
17


LNU263
25794.6
4.65
0.738
2
LNU43
14423.6
8.66
0.549
13
LNU278
25814.1
64.05
0.414
7


LNU267
25804.3
4.66
0.579
2
LNU43
14421.1
8.62
0.403
12
LNU278
25812.3
63.60
0.510
6


LNU271
25913.3
4.84
0.510
6
LNU45
25053.4
9.41
0.017
22
LNU36
25562.3
66.51
0.306
11


LNU271
25912.1
4.73
0.729
4
LNU67
25824.5
10.02
0.103
30
LNU43
14422.8
70.46
0.087
17


LNU278
25814.3
4.79
0.556
5
LNU67
25821.5
9.56
0.213
24
LNU43
14423.6
69.31
0.490
15


LNU278
25814.1
4.71
0.273
3
LNU67
25823.5
7.91
0.742
3
LNU43
14421.1
68.95
0.337
15


LNU278
25812.3
4.65
0.552
2
CONT.
—
5.51
—
0
LNU43
14422.9
61.94
0.683
3


LNU36
25562.3
4.84
0.280
6
LNU100
14472.1
7.25
0.214
32
LNU45
25053.4
75.26
0.020
25


LNU43
14421.1
5.03
0.265
10
LNU100
14473.1
6.68
0.126
21
LNU67
25824.5
80.13
0.080
33


LNU43
14423.6
4.98
0.534
9
LNU100
14473.3
6.65
0.202
21
LNU67
25821.5
76.49
0.178
27


LNU43
14422.8
4.91
0.059
8
LNU104
25032.2
8.31
0.061
51
LNU67
25823.5
63.25
0.582
5


LNU43
14422.9
4.69
0.341
3
LNU104
25033.3
7.18
0.094
30
LNU67
25821.4
61.30
0.796
2


LNU45
25053.4
5.08
0.048
12
LNU104
25032.1
6.09
0.137
10
CONT.
—
43.28
—
0


LNU67
25824.5
5.33
0.013
17
LNU106
14483.2
6.84
0.395
24
LNU100
14472.1
58.02
0.197
34


LNU67
25821.5
5.01
0.414
10
LNU106
14483.5
6.54
0.277
19
LNU100
14473.1
53.48
0.103
24


LNU67
25823.5
4.69
0.594
3
LNU106
14481.1
6.47
0.545
17
LNU100
14473.3
53.22
0.176
23


LNU67
25821.4
4.66
0.659
2
LNU106
14484.3
5.67
0.734
3
LNU104
25032.2
66.45
0.052
54


CONT.
—
4.04
—
0
LNU114
25041.2
7.36
0.173
34
LNU104
25033.3
57.45
0.078
33


LNU100
14472.1
4.66
0.122
15
LNU114
25044.4
6.15
0.537
12
LNU104
25032.1
48.73
0.095
13


LNU100
14473.1
4.55
0.001
12
LNU114
25041.1
5.89
0.758
7
LNU104
25033.8
44.10
0.672
2


LNU100
14473.3
4.45
0.361
10
LNU117
25931.4
6.66
0.186
21
LNU106
14483.2
54.74
0.371
26


LNU104
25032.2
4.96
0.095
23
LNU117
25931.1
5.91
0.423
7
LNU106
14483.5
52.35
0.247
21


LNU104
25033.3
4.73
0.150
17
LNU155
14523.5
5.99
0.663
9
LNU106
14481.1
51.74
0.511
20


LNU104
25032.1
4.25
0.102
5
LNU218
24781.4
6.59
0.002
20
LNU114
25041.2
58.92
0.158
36


LNU106
14483.2
4.55
0.371
13
LNU218
24781.6
6.06
0.519
10
LNU114
25044.4
49.23
0.487
14


LNU106
14481.1
4.50
0.556
11
LNU218
24781.1
5.78
0.271
5
LNU114
25041.1
47.15
0.701
9


LNU106
14483.5
4.40
0.277
9
LNU254
25782.4
6.21
0.006
13
LNU114
25042.1
45.58
0.794
5


LNU106
14484.3
4.18
0.404
3
LNU4
25133.3
6.38
0.118
16
LNU117
25931.4
53.31
0.161
23


LNU114
25041.2
4.64
0.072
15
LNU4
25134.3
6.31
0.001
14
LNU117
25931.1
47.29
0.337
9


LNU114
25044.4
4.22
0.627
4
LNU4
25134.2
6.01
0.517
9
LNU155
14523.5
47.93
0.605
11


LNU114
25041.1
4.20
0.685
4
LNU40
24792.1
7.46
0.301
35
LNU218
24781.4
52.74
0.001
22


LNU117
25931.4
4.55
0.106
12
LNU40
24794.4
6.15
0.536
12
LNU218
24781.6
48.47
0.459
12


LNU117
25931.1
4.15
0.711
3
LNU40
24794.3
5.94
0.458
8
LNU218
24781.1
46.21
0.160
7


LNU155
14523.5
4.30
0.554
6
LNU40
24792.2
5.64
0.769
2
LNU254
25782.4
49.64
0.004
15


LNU218
24781.4
4.53
0.000
12
LNU46
14462.5
7.95
0.292
44
LNU4
25133.3
51.05
0.090
18


LNU218
24781.6
4.39
0.371
9
LNU46
14462.1
7.10
0.000
29
LNU4
25134.3
50.45
0.001
17


LNU218
24781.1
4.24
0.138
5
LNU48
24801.4
7.08
0.007
28
LNU4
25134.2
48.07
0.452
11


LNU254
25782.4
4.26
0.046
5
LNU48
24802.1
6.43
0.546
17
LNU40
24792.1
59.71
0.285
38


LNU4
25133.3
4.50
0.233
11
LNU48
24803.2
5.82
0.623
6
LNU40
24794.4
49.18
0.486
14


LNU4
25134.2
4.26
0.337
5
LNU63
24814.2
6.82
0.060
24
LNU40
24794.3
47.49
0.377
10


LNU4
25134.3
4.25
0.069
5
LNU63
24814.6
6.19
0.055
12
LNU40
24792.2
45.13
0.611
4


LNU40
24792.1
4.82
0.229
19
LNU63
24812.3
5.68
0.410
3
LNU46
14462.5
63.58
0.280
47


LNU40
24794.4
4.40
0.436
9
LNU7
25081.1
6.87
0.077
25
LNU46
14462.1
56.79
0.000
31


LNU40
24794.3
4.20
0.287
4
LNU7
25083.1
5.88
0.710
7
LNU48
24801.4
53.00
0.046
22


LNU40
24792.2
4.16
0.413
3
LNU7
25083.3
5.67
0.536
3
LNU48
24802.1
51.43
0.511
19


LNU46
14462.5
4.78
0.258
18
LNU8
25063.1
6.90
0.262
25
LNU48
24803.2
46.55
0.528
8


LNU46
14462.1
4.60
0.009
14
LNU8
25062.1
6.81
0.405
24
LNU63
24814.2
54.54
0.044
26


LNU48
24801.4
4.57
0.000
13
LNU8
25062.2
6.00
0.024
9
LNU63
24814.6
49.55
0.035
14


LNU48
24802.1
4.44
0.480
10
LNU8
25061.2
5.96
0.360
8
LNU63
24812.3
45.47
0.234
5


LNU48
24803.2
4.12
0.714
2
LNU94
24833.1
6.98
0.006
27
LNU63
24814.7
44.44
0.562
3


LNU63
24814.2
4.54
0.028
12
LNU94
24831.4
6.89
0.092
25
LNU7
25081.1
54.97
0.061
27


LNU63
24814.6
4.48
0.004
11
LNU94
24833.3
6.88
0.101
25
LNU7
25083.1
47.07
0.643
9


LNU63
24814.7
4.13
0.375
2
LNU94
24834.4
6.54
0.594
19
LNU7
25083.3
45.32
0.338
5


LNU7
25081.1
4.63
0.039
14
LNU96
25071.2
6.37
0.261
16
LNU8
25063.1
55.17
0.240
27


LNU7
25083.1
4.32
0.569
7
LNU96
25073.4
6.26
0.027
14
LNU8
25062.1
51.57
0.580
19


LNU8
25063.1
4.59
0.202
13
LNU96
25071.3
6.25
0.601
13
LNU8
25062.2
47.97
0.015
11


LNU8
25062.1
4.43
0.453
10
CONT.
—
5.52
—
0
LNU8
25061.2
47.65
0.283
10


LNU8
25062.2
4.28
0.023
6
LNU10
25123.5
6.36
0.501
15
LNU94
24833.1
55.84
0.003
29


LNU8
25061.2
4.23
0.060
5
LNU122
25332.5
7.43
0.076
35
LNU94
24833.3
55.01
0.082
27


LNU94
24831.4
4.57
0.059
13
LNU122
25332.2
6.54
0.271
19
LNU94
24834.4
52.33
0.564
21


LNU94
24833.1
4.54
0.234
12
LNU122
25332.1
5.85
0.107
6
LNU94
24831.4
51.83
0.377
20


LNU94
24833.3
4.47
0.001
10
LNU125
25941.4
6.33
0.058
15
LNU96
25071.2
50.95
0.223
18


LNU94
24834.4
4.28
0.678
6
LNU125
25943.2
6.05
0.017
10
LNU96
25073.4
50.07
0.016
16


LNU96
25071.2
4.32
0.311
7
LNU178
14611.5
6.41
0.271
16
LNU96
25071.3
50.01
0.560
16


LNU96
25073.4
4.27
0.121
6
LNU178
14611.1
5.90
0.494
7
CONT.
—
44.13
—
0


LNU96
25071.3
4.23
0.729
5
LNU178
14612.1
5.77
0.771
5
LNU10
25123.5
50.86
0.501
15


CONT.
—
4.07
—
0
LNU234
25014.4
6.04
0.267
9
LNU122
25332.5
59.44
0.076
35


LNU10
25123.5
4.50
0.413
11
LNU236
25423.3
6.00
0.132
9
LNU122
25332.2
52.35
0.271
19


LNU122
25332.5
4.69
0.003
15
LNU236
25425.3
5.96
0.660
8
LNU122
25332.1
46.78
0.107
6


LNU122
25332.2
4.35
0.245
7
LNU236
25424.2
5.95
0.471
8
LNU125
25941.4
50.64
0.058
15


LNU122
25332.1
4.27
0.198
5
LNU236
25425.4
5.73
0.639
4
LNU125
25943.2
48.42
0.017
10


LNU125
25941.4
4.41
0.004
8
LNU236
25422.4
5.73
0.273
4
LNU178
14611.5
51.30
0.271
16


LNU125
25943.2
4.20
0.109
3
LNU25
14083.7
6.23
0.090
13
LNU178
14611.1
47.22
0.494
7


LNU178
14611.5
4.38
0.025
8
LNU25
14082.8
6.00
0.068
9
LNU178
14612.1
46.17
0.771
5


LNU178
14611.1
4.23
0.524
4
LNU271
25911.4
6.14
0.007
11
LNU234
25014.4
48.31
0.267
9


LNU234
25014.4
4.35
0.065
7
LNU278
25814.1
6.84
0.000
24
LNU236
25423.3
48.03
0.132
9


LNU236
25425.4
4.29
0.469
5
LNU278
25814.3
6.80
0.049
23
LNU236
25425.3
47.68
0.660
8


LNU236
25425.3
4.27
0.665
5
LNU278
25813.2
5.87
0.769
6
LNU236
25424.2
47.58
0.471
8


LNU236
25423.3
4.18
0.177
3
LNU278
25812.3
5.80
0.562
5
LNU236
25425.4
45.87
0.639
4


LNU25
14083.7
4.37
0.179
7
LNU278
25812.2
5.74
0.748
4
LNU236
25422.4
45.86
0.273
4


LNU25
14082.8
4.28
0.102
5
LNU43
14423.7
5.87
0.454
6
LNU25
14083.7
49.87
0.090
13


LNU267
25803.1
4.17
0.224
2
LNU43
14423.6
5.85
0.597
6
LNU25
14082.8
48.04
0.068
9


LNU271
25911.4
4.32
0.005
6
LNU45
25052.12
7.09
0.347
29
LNU271
25911.4
49.15
0.007
11


LNU271
25913.3
4.17
0.665
2
LNU45
25053.4
7.03
0.158
28
LNU278
25814.1
54.73
0.000
24


LNU278
25814.1
4.53
0.014
11
LNU45
25052.11
6.11
0.526
11
LNU278
25814.3
54.39
0.049
23


LNU278
25814.3
4.51
0.018
11
LNU45
25052.9
5.81
0.542
5
LNU278
25813.2
46.93
0.769
6


LNU278
25812.2
4.29
0.549
5
LNU67
25823.5
7.64
0.000
39
LNU278
25812.3
46.39
0.562
5


LNU278
25813.2
4.23
0.708
4
LNU67
25824.5
5.73
0.563
4
LNU278
25812.2
45.95
0.748
4


LNU278
25812.3
4.21
0.465
3
LNU67
25821.5
5.65
0.575
2
LNU43
14423.7
46.93
0.454
6


LNU43
14423.7
4.28
0.436
5
LNU9
25001.7
6.07
0.382
10
LNU43
14423.6
46.77
0.597
6


LNU43
14423.6
4.22
0.486
4
LNU9
25001.1
5.69
0.730
3
LNU45
25052.12
56.76
0.347
29


LNU45
25052.12
4.65
0.342
14
LNU9
25003.1
5.68
0.610
3
LNU45
25053.4
56.27
0.158
28


LNU45
25053.4
4.56
0.208
12
CONT.
—
7.52
—
0
LNU45
25052.11
48.88
0.526
11


LNU45
25052.11
4.24
0.619
4
LNU10
25123.6
9.66
0.315
29
LNU45
25052.9
46.47
0.542
5


LNU45
25052.9
4.21
0.378
3
LNU10
25123.5
8.34
0.102
11
LNU67
25823.5
61.12
0.000
39


LNU67
25823.5
4.72
0.004
16
LNU157
24982.8
7.91
0.274
5
LNU67
25824.5
45.84
0.563
4


LNU67
25824.3
4.23
0.447
4
LNU168
24753.5
8.14
0.367
8
LNU67
25821.5
45.21
0.575
2


LNU9
25001.7
4.29
0.201
6
LNU173
25451.1
8.31
0.009
10
LNU9
25001.7
48.58
0.382
10


LNU9
25003.1
4.19
0.138
3
LNU173
25451.2
7.86
0.634
5
LNU9
25001.1
45.53
0.730
3


LNU9
25001.1
4.15
0.729
2
LNU178
14611.5
9.41
0.002
25
LNU9
25003.1
45.46
0.610
3


CONT.
—
4.68
—
0
LNU178
14611.4
9.14
0.223
22
CONT.
—
60.15
—
0


LNU10
25123.6
5.27
0.289
13
LNU178
14611.1
9.08
0.135
21
LNU10
25123.6
77.30
0.315
29


LNU10
25123.5
4.98
0.007
7
LNU184
25393.1
9.32
0.512
24
LNU10
25123.5
66.75
0.102
11


LNU157
24982.8
4.91
0.084
5
LNU184
25393.2
9.21
0.123
22
LNU157
24982.8
63.29
0.274
5


LNU168
24753.5
4.93
0.301
6
LNU184
25394.3
9.18
0.443
22
LNU168
24753.5
65.11
0.367
8


LNU173
25451.1
5.21
0.016
11
LNU184
25395.1
8.66
0.155
15
LNU173
25451.1
66.45
0.009
10


LNU173
25451.2
4.77
0.648
2
LNU230
25415.1
10.23
0.024
36
LNU173
25451.2
62.87
0.634
5


LNU178
14611.5
5.23
0.136
12
LNU230
25413.2
9.09
0.162
21
LNU178
14611.5
75.30
0.002
25


LNU178
14611.4
5.15
0.052
10
LNU230
25412.1
8.84
0.000
18
LNU178
14611.4
73.11
0.223
22


LNU178
14611.1
5.05
0.206
8
LNU230
25413.1
8.51
0.025
13
LNU178
14611.1
72.61
0.135
21


LNU184
25394.3
5.28
0.400
13
LNU230
25412.2
8.44
0.068
12
LNU184
25393.1
74.53
0.512
24


LNU184
25393.1
5.26
0.428
12
LNU236
25425.4
9.76
0.202
30
LNU184
25393.2
73.66
0.123
22


LNU184
25393.2
5.14
0.273
10
LNU236
25424.2
9.53
0.078
27
LNU184
25394.3
73.42
0.443
22


LNU184
25395.1
5.09
0.015
9
LNU236
25423.3
8.78
0.364
17
LNU184
25395.1
69.24
0.155
15


LNU230
25415.1
5.42
0.002
16
LNU236
25422.4
8.71
0.006
16
LNU230
25415.1
81.83
0.024
36


LNU230
25413.2
5.11
0.006
9
LNU24
24974.2
8.96
0.272
19
LNU230
25413.2
72.72
0.162
21


LNU230
25412.1
5.07
0.001
8
LNU24
24971.2
8.70
0.001
16
LNU230
25412.1
70.74
0.000
18


LNU230
25413.1
4.97
0.028
6
LNU24
24971.3
8.53
0.547
13
LNU230
25413.1
68.11
0.025
13


LNU230
25412.2
4.97
0.186
6
LNU24
24972.1
8.05
0.085
7
LNU230
25412.2
67.54
0.068
12


LNU236
25425.4
5.38
0.204
15
LNU24
24971.4
7.76
0.788
3
LNU236
25425.4
78.05
0.202
30


LNU236
25424.2
5.33
0.121
14
LNU263
25794.8
9.47
0.222
26
LNU236
25424.2
76.22
0.078
27


LNU236
25423.3
5.08
0.329
9
LNU276
25433.3
9.55
0.001
27
LNU236
25423.3
70.24
0.364
17


LNU236
25422.4
4.95
0.044
6
LNU276
25431.1
7.87
0.282
5
LNU236
25422.4
69.67
0.006
16


LNU24
24971.2
5.23
0.032
12
LNU279
25481.3
9.22
0.414
23
LNU24
24974.2
71.68
0.272
19


LNU24
24974.2
5.09
0.352
9
LNU279
25481.4
8.87
0.167
18
LNU24
24971.2
69.57
0.001
16


LNU24
24971.3
5.03
0.556
8
LNU279
25481.5
8.81
0.413
17
LNU24
24971.3
68.22
0.547
13


LNU24
24972.1
4.81
0.229
3
LNU36
25562.3
7.66
0.786
2
LNU24
24972.1
64.42
0.085
7


LNU24
24971.4
4.80
0.482
3
LNU36
25562.7
7.66
0.594
2
LNU24
24971.4
62.06
0.788
3


LNU263
25794.8
5.31
0.121
14
LNU53
25674.1
9.58
0.175
27
LNU263
25794.8
75.78
0.222
26


LNU276
25433.3
5.25
0.002
12
LNU53
25674.2
7.84
0.722
4
LNU276
25433.3
76.41
0.001
27


LNU276
25431.1
4.81
0.282
3
LNU53
25674.6
7.72
0.545
3
LNU276
25431.1
62.93
0.282
5


LNU279
25481.4
5.08
0.321
9
LNU56
24693.1
8.44
0.356
12
LNU279
25481.3
73.77
0.414
23


LNU279
25481.3
5.02
0.552
7
LNU56
24694.1
8.33
0.056
11
LNU279
25481.4
70.96
0.167
18


LNU279
25481.5
5.01
0.447
7
LNU56
24693.2
7.76
0.556
3
LNU279
25481.5
70.48
0.413
17


LNU36
25562.3
4.96
0.429
6
LNU73
25755.1
9.11
0.194
21
LNU36
25562.3
61.29
0.786
2


LNU53
25674.1
5.35
0.188
14
LNU73
25751.1
8.87
0.641
18
LNU36
25562.7
61.26
0.594
2


LNU53
25674.2
4.85
0.112
4
LNU73
25754.2
8.51
0.642
13
LNU53
25674.1
76.62
0.175
27


LNU53
25674.6
4.85
0.235
4
LNU73
25751.8
8.46
0.566
12
LNU53
25674.2
62.69
0.722
4


LNU56
24693.1
5.02
0.358
7
LNU73
25751.9
8.14
0.255
8
LNU53
25674.6
61.73
0.545
3


LNU56
24694.1
4.95
0.147
6
LNU9
25001.1
9.43
0.119
25
LNU56
24693.1
67.51
0.356
12


LNU73
25751.1
5.16
0.600
10
LNU9
25001.7
9.08
0.159
21
LNU56
24694.1
66.68
0.056
11


LNU73
25755.1
5.13
0.188
10
LNU9
25001.2
8.80
0.315
17
LNU56
24693.2
62.11
0.556
3


LNU73
25754.2
5.05
0.562
8
LNU9
25001.3
8.59
0.527
14
LNU73
25755.1
72.87
0.194
21


LNU73
25751.8
5.02
0.539
7
CONT.
—
5.46
—
0
LNU73
25751.1
70.95
0.641
18


LNU73
25751.9
4.87
0.323
4
LNU131
14005.2
8.63
0.000
58
LNU73
25754.2
68.05
0.642
13


LNU9
25001.1
5.27
0.006
13
LNU131
14005.5
7.70
0.000
41
LNU73
25751.8
67.65
0.566
12


LNU9
25001.7
5.23
0.108
12
LNU131
14002.15
5.68
0.675
4
LNU73
25751.9
65.14
0.255
8


LNU9
25001.2
5.00
0.381
7
LNU135
26203.1
6.47
0.756
18
LNU9
25001.1
75.41
0.119
25


LNU9
25001.3
4.93
0.552
5
LNU135
26204.2
6.39
0.071
17
LNU9
25001.7
72.60
0.159
21


CONT.
—
3.89
—
0
LNU135
26203.4
6.30
0.740
15
LNU9
25001.2
70.42
0.315
17


LNU131
14005.2
4.95
0.001
27
LNU135
26203.6
5.91
0.651
8
LNU9
25001.3
68.74
0.527
14


LNU131
14005.5
4.66
0.000
20
LNU135
26203.3
5.59
0.723
2
CONT.
—
43.36
—
0


LNU135
26203.4
4.17
0.763
7
LNU161
14552.9
5.58
0.793
2
LNU131
14005.2
69.03
0.000
59


LNU135
26204.2
4.15
0.173
7
LNU173
25451.2
7.12
0.405
30
LNU131
14005.5
61.62
0.000
42


LNU135
26203.6
4.04
0.754
4
LNU173
25451.5
5.78
0.563
6
LNU131
14002.15
45.46
0.614
5


LNU173
25451.2
4.44
0.465
14
LNU181
25771.8
7.22
0.385
32
LNU135
26203.1
51.73
0.746
19


LNU181
25771.8
4.49
0.391
15
LNU181
25771.6
6.86
0.005
25
LNU135
26204.2
51.12
0.062
18


LNU181
25771.6
4.32
0.006
11
LNU181
25774.1
6.52
0.018
19
LNU135
26203.4
50.44
0.727
16


LNU181
25771.5
4.21
0.573
8
LNU181
25771.5
6.38
0.548
17
LNU135
26203.6
47.26
0.621
9


LNU181
25774.1
4.10
0.130
5
LNU184
25393.2
7.56
0.000
38
LNU135
26203.3
44.70
0.629
3


LNU184
25393.2
4.54
0.000
17
LNU184
25394.1
7.46
0.238
36
LNU161
14552.9
44.61
0.713
3


LNU184
25394.1
4.52
0.195
16
LNU184
25393.1
6.68
0.061
22
LNU173
25451.2
56.96
0.397
31


LNU184
25393.3
4.34
0.530
11
LNU184
25393.3
6.64
0.595
21
LNU173
25451.5
46.25
0.512
7


LNU184
25393.1
4.32
0.096
11
LNU184
25394.3
6.51
0.645
19
LNU181
25771.8
57.77
0.377
33


LNU184
25394.3
4.27
0.603
10
LNU184
25395.1
6.01
0.471
10
LNU181
25771.6
54.86
0.004
27


LNU184
25395.1
3.96
0.799
2
LNU224
25872.3
9.03
0.019
65
LNU181
25774.1
52.14
0.014
20


LNU224
25872.3
5.01
0.045
29
LNU224
25874.4
8.06
0.136
47
LNU181
25771.5
51.03
0.532
18


LNU224
25874.1
4.62
0.275
19
LNU224
25874.1
7.70
0.317
41
LNU184
25393.2
60.48
0.000
39


LNU224
25874.4
4.61
0.105
18
LNU224
25872.2
7.18
0.359
31
LNU184
25394.1
59.65
0.232
38


LNU224
25872.2
4.45
0.255
14
LNU224
25871.3
5.78
0.451
6
LNU184
25393.1
53.47
0.055
23


LNU246
25744.3
4.64
0.491
19
LNU246
25744.3
7.98
0.492
46
LNU184
25394.3
52.09
0.633
20


LNU246
25744.2
4.64
0.043
19
LNU246
25744.2
7.83
0.167
43
LNU184
25393.3
50.56
0.719
17


LNU246
25744.4
4.60
0.531
18
LNU246
25744.4
7.70
0.539
41
LNU184
25395.1
48.06
0.440
11


LNU246
25743.1
4.44
0.177
14
LNU246
25743.1
6.94
0.175
27
LNU224
25872.3
72.21
0.019
67


LNU246
25743.2
4.24
0.017
9
LNU246
25743.2
6.60
0.006
21
LNU224
25874.4
64.48
0.133
49


LNU250
25591.1
4.80
0.006
23
LNU250
25591.1
8.34
0.140
53
LNU224
25874.1
61.56
0.311
42


LNU250
25592.2
4.41
0.001
13
LNU250
25592.2
6.69
0.003
22
LNU224
25872.2
57.46
0.351
33


LNU260
26404.1
4.10
0.473
5
LNU260
26404.1
6.08
0.526
11
LNU224
25871.3
46.25
0.391
7


LNU276
25433.6
4.57
0.306
17
LNU260
26403.1
5.88
0.523
8
LNU246
25744.3
63.80
0.486
47


LNU276
25433.5
4.20
0.487
8
LNU276
25433.6
7.47
0.273
37
LNU246
25744.2
62.63
0.163
44


LNU279
25481.3
4.68
0.457
20
LNU276
25433.5
6.51
0.490
19
LNU246
25744.4
61.63
0.533
42


LNU279
25484.3
4.60
0.331
18
LNU276
25433.3
5.80
0.607
6
LNU246
25743.2
52.80
0.005
22


LNU279
25481.2
3.96
0.708
2
LNU279
25484.3
7.86
0.232
44
LNU246
25743.1
51.76
0.010
19


LNU3
26124.3
4.05
0.307
4
LNU279
25481.3
7.80
0.383
43
LNU250
25591.1
66.75
0.137
54


LNU33
25553.3
4.15
0.416
7
LNU279
25481.2
6.22
0.206
14
LNU250
25592.2
53.50
0.002
23


LNU53
25674.5
4.05
0.348
4
LNU279
25481.5
6.02
0.769
10
LNU260
26404.1
48.66
0.501
12


LNU56
24694.2
4.01
0.359
3
LNU3
26122.2
5.76
0.546
5
LNU260
26403.1
47.04
0.484
8


CONT.
—
4.57
—
0
LNU3
26124.3
5.71
0.636
4
LNU276
25433.6
59.76
0.267
38


LNU130
24912.7
5.24
0.000
15
LNU33
25553.3
6.36
0.406
16
LNU276
25433.5
52.07
0.476
20


LNU130
24913.5
4.81
0.452
5
LNU33
25552.2
5.73
0.453
5
LNU276
25433.3
46.39
0.563
7


LNU130
24911.7
4.79
0.709
5
LNU53
25674.5
6.02
0.129
10
LNU279
25484.3
62.91
0.228
45


LNU136
14515.1
5.09
0.087
12
LNU56
24694.2
5.79
0.346
6
LNU279
25481.3
62.39
0.377
44


LNU142
27541.1
4.78
0.662
5
CONT.
—
7.19
—
0
LNU279
25481.2
49.74
0.186
15


LNU15
14123.13
4.85
0.095
6
LNU119
26142.8
7.51
0.405
4
LNU279
25481.5
48.15
0.752
11


LNU15
14123.11
4.83
0.046
6
LNU130
24912.7
8.81
0.058
23
LNU3
26122.2
46.10
0.489
6


LNU185
26475.1
4.91
0.486
8
LNU130
24913.5
7.84
0.354
9
LNU3
26124.3
45.68
0.578
5


LNU185
26474.2
4.77
0.091
4
LNU130
24911.7
7.63
0.793
6
LNU33
25553.3
50.90
0.388
17


LNU212
25834.5
4.87
0.163
7
LNU130
24913.6
7.54
0.613
5
LNU33
25552.2
45.87
0.382
6


LNU212
25834.1
4.85
0.527
6
LNU136
14515.1
8.58
0.046
19
LNU53
25674.5
48.16
0.103
11


LNU216
25985.4
4.95
0.714
8
LNU142
27541.1
8.08
0.487
12
LNU56
24694.2
46.36
0.287
7


LNU216
25982.1
4.72
0.350
3
LNU142
27545.1
7.62
0.787
6
CONT.
—
57.54
—
0


LNU216
25982.2
4.65
0.766
2
LNU142
27546.1
7.55
0.280
5
LNU119
26142.8
60.06
0.405
4


LNU228
26222.4
4.93
0.168
8
LNU15
14123.13
8.21
0.064
14
LNU130
24912.7
70.52
0.058
23


LNU228
26224.7
4.76
0.623
4
LNU15
14123.11
7.71
0.257
7
LNU130
24913.5
62.68
0.354
9


LNU241
26232.4
4.79
0.104
5
LNU185
26475.1
8.28
0.371
15
LNU130
24911.7
61.01
0.793
6


LNU241
26233.2
4.77
0.290
4
LNU185
26474.2
7.75
0.352
8
LNU130
24913.6
60.30
0.613
5


LNU277
25845.1
4.73
0.691
4
LNU212
25834.5
8.45
0.003
18
LNU136
14515.1
68.61
0.046
19


LNU280
26162.1
5.13
0.547
12
LNU212
25834.1
7.98
0.668
11
LNU142
27541.1
64.66
0.487
12


LNU280
26162.7.
4.79
0.255
5
LNU216
25985.4
8.64
0.625
20
LNU142
27545.1
60.95
0.787
6


LNU55
26015.1
4.82
0.304
5
LNU216
25982.1
7.69
0.532
7
LNU142
27546.1
60.40
0.280
5


LNU81
26034.3
5.23
0.040
14
LNU216
25982.2
7.54
0.539
5
LNU15
14123.13
65.70
0.064
14


CONT.
—
4.91
—
0
LNU228
26222.4
8.01
0.037
11
LNU15
14123.11
61.64
0.257
7


LNU119
26141.1
5.35
0.525
9
LNU228
26224.7
7.58
0.588
5
LNU185
26475.1
66.26
0.371
15


LNU119
26142.8
5.22
0.246
6
LNU241
26233.2
7.94
0.271
10
LNU185
26474.2
61.98
0.352
8


LNU130
24914.5
5.30
0.068
8
LNU274
26265.1
7.38
0.559
3
LNU212
25834.5
67.62
0.003
18


LNU136
14515.1
5.19
0.363
6
LNU277
25845.1
8.19
0.506
14
LNU212
25834.1
63.81
0.668
11


LNU142
27541.2
5.19
0.243
6
LNU280
26162.1
9.03
0.532
26
LNU216
25985.4
69.11
0.625
20


LNU142
27541.
5.18
0.641
6
LNU280
26162.7
7.81
0.372
9
LNU216
25982.1
61.50
0.532
7


LNU142
27545.1
5.11
0.039
4
LNU55
26015.1
7.57
0.495
5
LNU216
25982.2
60.31
0.539
5


LNU15
14123.11
5.64
0.450
15
LNU55
26015.3
7.33
0.676
2
LNU228
26222.4
64.07
0.037
11


LNU15
14122.8
5.43
0.709
11
LNU81
26034.3
9.24
0.001
28
LNU228
26224.7
60.67
0.588
5


LNU15
14123.13
5.36
0.081
9
CONT.
—
8.21
—
0
LNU241
26233.2
63.55
0.271
10


LNU212
25834.5
5.27
0.020
7
LNU119
26142.8
9.21
0.257
12
LNU274
26265.1
59.07
0.559
3


LNU212
25834.1
5.09
0.469
4
LNU119
26141.1
8.81
0.725
7
LNU277
25845.1
65.53
0.506
14


LNU212
25834.4
5.04
0.481
3
LNU130
24914.5
9.08
0.160
11
LNU280
26162.1
72.23
0.532
26


LNU216
25984.6
5.11
0.290
4
LNU130
24913.5
8.41
0.792
2
LNU55
26015.1
60.59
0.495
5


LNU216
25984.1
5.11
0.459
4
LNU136
14515.1
8.81
0.488
7
LNU55
26015.3
58.66
0.676
2


LNU228
26224.7
5.49
0.069
12
LNU142
27541.1
8.99
0.680
10
LNU81
26034.3
73.89
0.001
28


LNU228
26222.1
5.32
0.001
8
LNU142
27541.2
8.81
0.265
7
CONT.
—
65.65
—
0


LNU229
26111.5
5.30
0.002
8
LNU142
27545.1
8.76
0.036
7
LNU119
26142.8
73.65
0.257
12


LNU241
26232.4
5.31
0.438
8
LNU149
26174.7
8.81
0.799
7
LNU130
24914.5
72.61
0.160
11


LNU274
26262.2
5.10
0.464
4
LNU15
14123.11
10.26
0.454
25
LNU130
24913.5
67.25
0.792
2


LNU277
25842.3
5.28
0.524
7
LNU15
14122.8
10.12
0.624
23
LNU136
14515.1
70.47
0.488
7


LNU280
26164.4
5.21
0.005
6
LNU15
14123.13
9.22
0.424
12
LNU142
27541.1
71.91
0.680
10


LNU280
26164.3
5.11
0.061
4
LNU212
25834.5
9.20
0.260
12
LNU142
27541.2
70.52
0.265
7


LNU280
26162.1
5.10
0.346
4
LNU212
25834.4
8.59
0.703
5
LNU142
27545.1
70.07
0.036
7


LNU81
26031.10
5.59
0.339
14
LNU216
25984.1
9.00
0.597
10
LNU149
26174.7
70.46
0.799
7


LNU81
26034.3
5.12
0.770
4
LNU216
25982.2
8.73
0.147
6
LNU15
14123.11
82.11
0.454
25


LNU81
26034.2
5.12
0.022
4
LNU216
25984.6
8.55
0.486
4
LNU15
14122.8
80.97
0.624
23


CONT.

4.203
—
0.0
LNU228
26222.1
9.79
0.004
19
LNU15
14123.13
73.80
0.424
12


LNU61
26134.4
4.780
0.02
13.7
LNU228
26224.7
9.60
0.018
17
LNU212
25834.5
73.62
0.260
12


CONT.
—
4.203
—
0.0
LNU229
26111.5
8.83
0.421
8
LNU212
25834.4
68.74
0.703
5


LNU134
29191.3
4.344
<0.8
3.3
LNU241
26232.4
9.50
0.384
16
LNU216
25984.1
71.97
0.597
10


LNU134
29191.6
4.524
<0.3
7.6
LNU274
26262.2
8.45
0.409
3
LNU216
25982.2
69.80
0.147
6


LNU134
29194.1
4.365
<0.7
3.8
LNU277
25842.3
9.21
0.560
12
LNU216
25984.6
68.39
0.486
4


LNU198
27735.4
4.406
<0.7
4.8
LNU280
26164.4
9.50
0.000
16
LNU228
26222.1
78.34
0.004
19


LNU200
27992.2
4.704
<0.1
11.9
LNU280
26162.1
8.65
0.122
5
LNU228
26224.7
76.83
0.018
17


LNU200
27992.3
4.608
<0.3
9.6
LNU81
26031.10
10.53
0.247
28
LNU229
26111.5
70.61
0.421
8


LNU200
27993.4
4.735
<0.1
12.6
LNU81
26034.3
8.79
0.743
7
LNU241
26232.4
76.03
0.384
16


LNU200
27994.3
4.378
<0.8
4.1
LNU81
26031.9
8.48
0.768
3
LNU274
26262.2
67.61
0.409
3


LNU244
28013.6
4.584
<0.3
9.1
CONT.

5.508
—
0.0
LNU277
25842.3
73.68
0.560
12


LNU244
28013.8
4.635
<0.3
10.3
LNU61
26134.4
6.634
0.045
20.5
LNU280
26164.4
76.02
0.000
16


LNU244
28014.4
4.673
<0.1
11.2
LNU61
26135.3
5.827
0.55
5.8
LNU280
26162.1
69.21
0.122
5


LNU244
28015.1
4.628
<0.3
10.1
CONT.
—
5.508
—
0.0
LNU81
26031.10
84.26
0.247
28


LNU262
27591.3
4.517
<0.3
7.5
LNU123
27722.4
5.740
<0.8
4.2
LNU81
26034.3
70.32
0.743
7


LNU262
27591.7
4.740
<0.1
12.8
LNU134
29191.3
5.789
<0.8
5.1
LNU81
26031.9
67.84
0.768
3


LNU262
27593.1
4.860
<0.1
15.6
LNU134
29191.6
6.647
<0.1
20.7
CONT.

44.061
—
0.0


LNU262
27593.6
4.849
<0.1
15.4
LNU134
29194.1
6.208
<0.4
12.7
LNU61
26134.4
53.071
0.045
20.5


LNU262
27595.2
4.884
<0.1
16.2
LNU198
27735.4
6.147
<0.4
11.6
LNU61
26135.3
46.614
0.55
5.8


LNU266
27931.5
4.544
<0.3
8.1
LNU200
27992.2
6.852
<0.1
24.4
CONT.
—
44.061
—
0.0


LNU266
27932.1
4.312
<0.8
2.6
LNU200
27992.3
6.540
<0.1
18.7
LNU123
27722.4
45.924
<0.7
4.2


LNU266
27935.3
4.632
<0.3
10.2
LNU200
27993.4
6.516
<0.1
18.3
LNU134
29191.6
53.174
<0.1
20.7


LNU266
27935.4
4.449
<0.7
5.8
LNU200
27994.3
6.021
<0.4
9.3
LNU134
29194.1
49.666
<0.3
12.7


LNU29
27652.2
4.297
<0.8
2.2
LNU244
28013.6
6.619
<0.1
20.2
LNU198
27735.4
49.176
<0.7
11.6


LNU29
27653.1
4.758
<0.1
13.2
LNU244
28013.8
6.593
<0.1
19.7
LNU200
27992.2
54.815
<0.1
24.4


LNU29
27654.1
4.748
<0.1
13.0
LNU244
28014.4
6.854
<0.1
24.4
LNU200
27992.3
52.322
<0.1
18.7


LNU29
27655.1
4.751
<0.1
13.0
LNU244
28015.1
6.623
<0.1
20.3
LNU200
27993.4
52.124
<0.1
18.3


LNU32
29252.6
4.427
<0.3
5.3
LNU262
27591.3
6.548
<0.1
18.9
LNU200
27994.3
48.167
<0.7
9.3


LNU51
27612.2
4.681
<0.1
11.4
LNU262
27591.7
6.654
<0.1
20.8
LNU244
28013.6
52.951
<0.1
20.2


LNU51
27614.4
4.482
<0.3
6.6
LNU262
27593.1
7.392
<0.1
34.2
LNU244
28013.8
52.747
<0.1
19.7


LNU51
27616.1
4.677
<0.3
11.3
LNU262
27593.6
7.160
<0.1
30.0
LNU244
28014.4
54.828
<0.1
24.4


LNU58
27673.4
4.505
<0.3
7.2
LNU262
27595.2
7.217
<0.1
31.0
LNU244
28015.1
52.986
<0.1
20.3


CONT.
8252.24
2.097

0.0
LNU266
27931.5
6.158
<0.4
11.8
LNU262
27591.3
52.382
<0.1
18.9


LNU89
25325.2
2.217
0.7
5.7
LNU266
27932.1
5.966
<0.4
8.3
LNU262
27591.7
53.232
<0.1
20.8







LNU266
27935.3
6.563
<0.1
19.2
LNU262
27593.1
59.137
<0.1
34.2







LNU266
27935.4
6.305
<0.4
14.5
LNU262
27593.6
57.278
<0.1
30.0







LNU29
27653.1
7.013
<0.1
27.3
LNU262
27595.2
57.740
<0.1
31.0







LNU29
27654.1
6.500
<0.1
18.0
LNU266
27931.5
49.265
<0.7
11.8







LNU29
27655.1
6.526
<0.1
18.5
LNU266
27932.1
47.729
<0.7
8.3







LNU32
29251.1
5.912
<0.8
7.3
LNU266
27935.3
52.505
<0.1
19.2







LNU32
29252.6
6.123
<0.4
11.2
LNU266
27935.4
50.439
<0.3
14.5







LNU51
27612.2
6.630
<0.1
20.4
LNU29
27653.1
56.105
<0.1
27.3







LNU51
27614.4
6.359
<0.4
15.5
LNU29
27654.1
51.998
<0.1
18.0







LNU51
27616.1
6.922
<0.1
25.7
LNU29
27655.1
52.211
<0.1
18.5







LNU58
27673.2
5.906
<0.8
7.2
LNU32
29251.1
47.296
<0.7
7.3







LNU58
27673.4
6.527
<0.1
18.5
LNU32
29252.6
48.987
<0.7
11.2












LNU51
27612.2
53.044
<0.1
20.4












LNU51
27614.4
50.868
<0.1
15.5












LNU51
27616.1
55.376
<0.1
25.7












LNU58
27673.2
47.248
<0.3
7.2












LNU58
27673.4
52.219
<0.15
18.5












CONT.
8252.24
10.791

0.0












LNU89
25325.1
11.960
0.3
10.8












LNU89
25325.2
11.480
0.54
6.4





Table 83.


“CONT.”—Control;


“Ave.”—Average;


“% Incr.” = % increment.






The genes listed in Table 84 improved plant NUE when grown at standard nitrogen concentration levels. These genes produced larger photosynthetic areas as it can be observed by their larger leaf blade area and leaf petiole length. The genes were cloned under the regulation of a constitutive (At6669) and root preferred promoter (RootP). The evaluation of each gene was performed by testing the performance of different number of events. Event with p-value <0.1 was considered statistically significant







TABLE 84







Genes showing improved photosynthetic capacity at standard nitrogen growth


conditions















Leaf Blade Area

Leaf Petiole



Leaf Number

[cm2]

Length [cm]





















Gene


P-
%
Gene


P-
%
Gene


P-
%


Name
Event #
Ave.
Value
incr.
Name
Event #
Ave.
Value
incr.
Name
Event #
Ave.
Value
incr.
























CONT.
—
10.49
—
0
CONT.
—
0.64
—
0
CONT.
—
0.76
—
0


LNU100
14471.4
11.25
0.262
7
LNU100
14471.4
0.81
0.306
28
LNU100
14471.4
0.88
0.214
16


LNU100
14473.3
11.13
0.024
6
LNU100
14474.3
0.77
0.003
21
LNU100
14473.3
0.87
0.001
14


LNU100
14474.3
11.13
0.024
6
LNU100
14473.3
0.77
0.117
20
LNU100
14472.2
0.85
0.002
11


LNU100
14472.2
10.75
0.472
2
LNU100
14472.2
0.75
0.256
17
LNU100
14474.3
0.84
0.102
10


LNU100
14474.4
10.69
0.639
2
LNU104
25032.2
0.91
0.059
43
LNU104
25033.1
0.96
0.055
26


LNU104
25032.2
12.13
0.001
16
LNU104
25033.1
0.89
0.159
39
LNU104
25032.2
0.95
0.179
25


LNU104
25034.1
11.00
0.048
5
LNU104
25032.1
0.88
0.076
38
LNU104
25033.3
0.90
0.002
18


LNU104
25032.1
10.94
0.631
4
LNU104
25033.3
0.84
0.007
31
LNU104
25032.1
0.89
0.157
17


LNU104
25033.3
10.94
0.015
4
LNU104
25034.1
0.70
0.146
10
LNU104
25034.1
0.80
0.437
4


LNU104
25033.1
10.69
0.639
2
LNU106
14482.3
0.79
0.371
24
LNU106
14482.3
0.90
0.441
19


LNU106
14483.5
11.06
0.115
5
LNU106
14483.5
0.78
0.140
22
LNU106
14483.5
0.90
0.000
17


LNU106
14482.3
10.88
0.329
4
LNU106
14483.2
0.76
0.264
20
LNU106
14483.2
0.83
0.043
8


LNU106
14483.2
10.75
0.610
2
LNU106
14481.1
0.66
0.416
3
LNU114
25041.1
0.99
0.000
29


LNU114
25041.1
11.94
0.000
14
LNU114
25041.1
0.86
0.000
35
LNU114
25042.1
0.85
0.000
12


LNU114
25041.2
11.00
0.048
5
LNU114
25042.1
0.77
0.122
21
LNU114
25041.2
0.84
0.250
10


LNU114
25042.1
10.69
0.222
2
LNU114
25041.2
0.70
0.018
9
LNU155
14525.1
0.85
0.540
12


LNU213
24653.2
11.50
0.090
10
LNU155
14525.6
0.78
0.126
23
LNU155
14525.6
0.85
0.231
11


LNU213
24653.1
10.75
0.472
2
LNU155
14525.1
0.71
0.598
12
LNU213
24653.2
1.00
0.000
32


LNU218
24781.2
11.00
0.486
5
LNU213
24653.2
0.88
0.000
39
LNU213
24652.4
0.88
0.468
15


LNU218
24781.1
10.81
0.591
3
LNU213
24652.4
0.83
0.472
31
LNU213
24653.1
0.83
0.200
8


LNU218
24781.7
10.75
0.230
2
LNU213
24653.1
0.74
0.000
16
LNU218
24781.7
0.91
0.091
20


LNU23
25163.5
11.06
0.401
5
LNU213
24654.4
0.68
0.754
7
LNU218
24781.1
0.89
0.004
16


LNU28
25171.1
10.88
0.688
4
LNU218
24781.1
0.82
0.211
29
LNU218
24781.2
0.85
0.001
11


LNU28
25171.2
10.88
0.102
4
LNU218
24781.7
0.80
0.254
25
LNU23
25163.5
0.91
0.234
19


LNU4
25134.1
11.25
0.262
7
LNU218
24781.2
0.70
0.232
11
LNU23
25163.6
0.83
0.193
9


LNU4
25134.3
11.06
0.282
5
LNU23
25163.5
0.90
0.028
41
LNU28
25171.2
0.89
0.143
16


LNU4
25133.3
10.94
0.015
4
LNU23
25163.6
0.70
0.546
9
LNU28
25171.1
0.80
0.064
5


LNU4
25134.2
10.69
0.639
2
LNU28
25171.2
0.81
0.201
28
LNU28
25171.4
0.79
0.610
4


LNU40
24794.4
10.88
0.102
4
LNU28
25174.3
0.78
0.071
23
LNU28
25174.3
0.78
0.381
2


LNU46
14464.4
11.69
0.196
11
LNU28
25171.1
0.72
0.240
13
LNU4
25134.1
0.89
0.116
16


LNU46
14463.1
11.31
0.371
8
LNU28
25174.5
0.68
0.508
7
LNU4
25133.3
0.86
0.225
12


LNU46
14462.5
11.19
0.079
7
LNU28
25171.4
0.65
0.790
3
LNU4
25134.3
0.83
0.476
9


LNU48
24802.2
12.00
0.182
14
LNU4
25134.1
0.86
0.062
35
LNU4
25134.2
0.80
0.164
4


LNU48
24801.4
11.25
0.000
7
LNU4
25133.3
0.77
0.000
22
LNU40
24794.3
0.88
0.322
15


LNU48
24802.1
11.13
0.001
6
LNU4
25134.3
0.76
0.419
20
LNU40
24794.4
0.86
0.221
13


LNU63
24812.2
11.06
0.282
5
LNU4
25131.1
0.69
0.563
9
LNU40
24792.1
0.81
0.029
6


LNU63
24814.2
10.94
0.483
4
LNU4
25134.2
0.69
0.404
8
LNU46
14463.1
0.92
0.307
21


LNU7
25083.1
11.13
0.024
6
LNU40
24794.3
0.83
0.013
30
LNU46
14464.4
0.92
0.125
21


LNU7
25082.2
11.00
0.384
5
LNU40
24794.4
0.78
0.248
22
LNU46
14462.5
0.89
0.001
17


LNU7
25081.1
10.81
0.060
3
LNU40
24792.1
0.70
0.011
10
LNU46
14462.1
0.80
0.321
4


LNU7
25082.7
10.69
0.473
2
LNU40
24792.2
0.67
0.582
6
LNU48
24801.4
0.95
0.000
25


LNU8
25062.2
10.88
0.647
4
LNU46
14463.1
0.83
0.288
30
LNU48
24802.2
0.92
0.214
21


LNU8
25063.1
10.75
0.748
2
LNU46
14464.4
0.83
0.258
30
LNU48
24802.1
0.90
0.216
17


LNU8
25063.6
10.69
0.639
2
LNU46
14462.5
0.82
0.000
29
LNU63
24814.2
0.87
0.489
14


LNU94
24833.3
11.13
0.001
6
LNU46
14462.1
0.75
0.214
17
LNU63
24812.2
0.83
0.441
9


LNU94
24831.4
11.00
0.048
5
LNU48
24801.4
0.93
0.004
46
LNU63
24811.2
0.80
0.686
5


LNU94
24834.4
10.69
0.473
2
LNU48
24802.1
0.81
0.017
28
LNU7
25081.1
0.90
0.013
17


LNU96
25071.3
11.06
0.004
5
LNU48
24802.2
0.81
0.000
27
LNU7
25083.3
0.84
0.415
10


LNU96
25073.3
11.03
0.530
5
LNU63
24814.2
0.81
0.376
28
LNU7
25083.1
0.82
0.009
8


LNU96
25073.4
10.69
0.222
2
LNU63
24812.2
0.72
0.265
13
LNU8
25063.6
0.85
0.045
12


CONT.
—
11.17
—
0
LNU63
24814.3
0.71
0.507
12
LNU8
25062.2
0.80
0.132
5


LNU124
14501.1
11.56
0.231
4
LNU63
24811.2
0.68
0.730
7
LNU94
24831.4
0.86
0.027
12


LNU132
14103.9
11.38
0.297
2
LNU7
25081.1
0.78
0.003
22
LNU94
24834.4
0.84
0.002
9


LNU140
14112.7
11.38
0.552
2
LNU7
25083.1
0.76
0.003
20
LNU94
24833.3
0.79
0.598
4


LNU37
14064.7
11.56
0.010
4
LNU7
25083.3
0.69
0.445
9
LNU96
25071.3
0.81
0.395
7


LNU72
24962.3
11.69
0.002
5
LNU7
25082.2
0.65
0.268
3
LNU96
25073.4
0.81
0.464
7


LNU82
24823.1
11.75
0.221
5
LNU8
25063.6
0.77
0.240
21
LNU96
25073.3
0.81
0.763
6


LNU84
25621.2
11.38
0.297
2
LNU8
25062.2
0.72
0.202
13
CONT.
—
0.84
—
0


LNU87
24712.4
11.44
0.761
2
LNU8
25063.1
0.68
0.036
7
LNU5
14042.7
0.91
0.264
8


LNU98
25762.2
11.75
0.045
5
LNU8
25061.2
0.68
0.124
6
LNU72
24962.3
0.91
0.559
8


CONT.
—
10.96
—
0
LNU94
24833.3
0.79
0.280
25
LNU87
24714.3
0.87
0.753
3


LNU124
14504.5
11.38
0.076
4
LNU94
24834.4
0.75
0.015
19
LNU98
25763.2
0.86
0.757
2


LNU148
25685.6
11.81
0.567
8
LNU94
24831.4
0.74
0.005
17
CONT.
—
0.76
—
0


LNU148
25685.2
11.63
0.140
6
LNU94
24833.1
0.71
0.624
11
LNU113
25631.1
0.92
0.325
20


LNU148
25683.2
11.19
0.459
2
LNU96
25073.3
0.75
0.357
17
LNU113
25631.3
0.85
0.570
11


LNU37
14064.7
11.25
0.198
3
LNU96
25071.3
0.71
0.238
12
LNU124
14504.5
0.84
0.388
11


LNU5
14043.9
11.19
0.603
2
LNU96
25073.4
0.67
0.521
6
LNU132
14102.9
0.86
0.489
12


LNU68
14034.13
11.56
0.092
6
CONT.
—
0.92
—
0
LNU140
14115.1
0.78
0.771
2


LNU68
14033.9
11.31
0.562
3
LNU148
25685.6
0.97
0.438
6
LNU148
25685.1
0.83
0.065
8


LNU68
14034.1
11.31
0.641
3
LNU5
14042.7
1.01
0.003
9
LNU148
25683.2
0.79
0.454
3


LNU71
25852.5
11.75
0.475
7
LNU72
24962.3
0.99
0.393
8
LNU287
24674.3
0.81
0.628
7


LNU71
25851.4
11.25
0.269
3
LNU98
25763.2
0.98
0.024
7
LNU37
14064.7
0.97
0.300
27


LNU74
25441.2
11.56
0.018
6
CONT.
—
0.82
—
0
LNU37
14064.6
0.93
0.434
21


LNU74
25444.1
11.38
0.127
4
LNU113
25631.3
1.00
0.210
23
LNU5
14043.9
0.81
0.406
6


LNU74
25443.2
11.25
0.443
3
LNU113
25631.1
0.98
0.043
19
LNU5
14043.7
0.78
0.587
2


LNU87
24712.1
11.25
0.665
3
LNU124
14504.5
0.91
0.218
11
LNU65
24703.6
0.89
0.001
17


CONT.
—
11.68
—
0
LNU132
14102.9
0.89
0.737
9
LNU65
24702.3
0.82
0.643
8


LNU117
25931.4
12.13
0.429
4
LNU148
25685.1
0.97
0.016
18
LNU68
14034.13
0.90
0.525
18


LNU117
25933.3
12.13
0.281
4
LNU148
25685.2
0.91
0.781
11
LNU68
14034.1
0.83
0.225
8


LNU122
25332.2
12.63
0.009
8
LNU287
24674.3
0.87
0.766
7
LNU71
25852.5
0.93
0.211
21


LNU122
25332.5
12.63
0.078
8
LNU37
14064.7
1.02
0.001
25
LNU71
25853.1
0.89
0.012
16


LNU122
25333.1
12.19
0.066
4
LNU37
14064.6
0.94
0.579
14
LNU71
25851.4
0.88
0.005
15


LNU122
25333.2
12.19
0.313
4
LNU5
14043.9
1.04
0.159
27
LNU72
24963.7
0.90
0.324
18


LNU122
25332.1
12.13
0.123
4
LNU5
14043.7
0.85
0.539
4
LNU72
24962.3
0.80
0.774
5


LNU125
25944.3
12.25
0.633
5
LNU65
24702.3
0.91
0.471
11
LNU74
25443.3
0.92
0.166
20


LNU25
14082.8
12.19
0.646
4
LNU65
24703.6
0.88
0.539
7
LNU82
24823.1
0.81
0.171
6


LNU267
25804.3
12.25
0.525
5
LNU68
14034.13
1.07
0.318
30
LNU84
25621.2
0.78
0.779
3


LNU45
25053.4
12.13
0.281
4
LNU71
25853.1
1.03
0.001
26
LNU84
25621.4
0.78
0.734
2


LNU67
25821.5
12.31
0.238
5
LNU71
25852.5
0.99
0.014
22
LNU87
24712.1
0.84
0.600
10


CONT.
—
10.39
—
0
LNU71
25851.4
0.95
0.352
16
LNU98
25763.2
0.81
0.213
6


LNU100
14472.1
11.19
0.381
8
LNU72
24963.7
0.95
0.423
16
CONT.
—
0.84
—
0


LNU100
14473.1
11.19
0.065
8
LNU74
25443.3
0.95
0.507
16
LNU117
25933.3
1.05
0.197
25


LNU100
14473.3
11.13
0.000
7
LNU82
24823.1
0.89
0.342
9
LNU117
25931.4
1.00
0.145
20


LNU104
25032.1
11.81
0.165
14
LNU84
25621.2
0.93
0.311
13
LNU117
25931.1
0.92
0.407
10


LNU104
25032.2
11.69
0.000
13
LNU84
25621.4
0.91
0.089
11
LNU117
25931.2
0.87
0.716
3


LNU104
25033.3
11.56
0.030
11
CONT.
—
1.01
—
0
LNU117
25932.4
0.86
0.577
3


LNU104
25033.8
10.94
0.416
5
LNU117
25931.4
1.38
0.070
37
LNU122
25332.5
0.95
0.368
13


LNU104
25033.1
10.88
0.576
5
LNU117
25933.3
1.26
0.013
25
LNU122
25332.1
0.95
0.014
13


LNU106
14483.5
11.00
0.028
6
LNU117
25932.4
1.10
0.235
8
LNU122
25333.1
0.92
0.412
9


LNU106
14483.2
10.94
0.416
5
LNU117
25931.1
1.09
0.286
8
LNU122
25332.2
0.89
0.313
6


LNU106
14484.3
10.78
0.451
4
LNU117
25931.2
1.04
0.773
2
LNU122
25333.2
0.88
0.182
5


LNU114
25041.2
11.31
0.049
9
LNU122
25333.2
1.24
0.029
23
LNU125
25941.4
0.98
0.080
17


LNU114
25041.1
11.13
0.149
7
LNU122
25332.2
1.20
0.063
19
LNU125
25944.3
0.97
0.096
15


LNU114
25042.1
10.69
0.063
3
LNU122
25333.1
1.20
0.045
19
LNU125
25941.2
0.88
0.608
5


LNU117
25931.4
10.94
0.297
5
LNU122
25332.5
1.15
0.331
14
LNU138
14074.6
0.98
0.005
17


LNU155
14525.1
11.06
0.001
6
LNU122
25332.1
1.14
0.392
13
LNU138
14074.5
0.97
0.064
15


LNU218
24781.2
10.94
0.568
5
LNU125
25941.4
1.28
0.010
26
LNU180
24723.1
0.89
0.138
7


LNU218
24781.6
10.94
0.128
5
LNU125
25941.2
1.06
0.598
5
LNU180
24724.1
0.89
0.519
6


LNU218
24781.1
10.88
0.255
5
LNU138
14074.5
1.24
0.018
23
LNU180
24722.2
0.87
0.400
4


LNU254
25782.4
11.56
0.000
11
LNU138
14074.6
1.23
0.080
22
LNU180
24721.2
0.87
0.704
3


LNU4
25134.1
11.63
0.000
12
LNU180
24722.2
1.07
0.420
6
LNU220
25405.6
0.93
0.053
11


LNU4
25133.3
11.06
0.239
6
LNU220
25405.1
1.11
0.197
10
LNU230
25413.2
0.96
0.537
14


LNU4
25131.1
11.00
0.427
6
LNU220
25405.3
1.08
0.473
7
LNU230
25412.2
0.91
0.548
8


LNU4
25134.3
10.88
0.255
5
LNU220
25405.6
1.07
0.722
6
LNU230
25413.1
0.90
0.566
8


LNU40
24792.1
11.56
0.030
11
LNU230
25413.1
1.26
0.345
25
LNU230
25412.1
0.87
0.343
4


LNU40
24794.3
10.88
0.255
5
LNU230
25412.2
1.20
0.501
19
LNU25
14082.8
0.97
0.007
16


LNU40
24794.4
10.81
0.381
4
LNU230
25413.2
1.16
0.643
15
LNU25
14082.9
0.93
0.069
11


LNU46
14462.5
11.50
0.082
11
LNU25
14083.7
1.14
0.520
12
LNU25
14083.1
0.93
0.025
11


LNU46
14462.1
11.06
0.089
6
LNU25
14082.9
1.10
0.226
9
LNU254
25782.4
0.92
0.529
9


LNU48
24802.2
11.50
0.004
11
LNU25
14083.1
1.10
0.227
9
LNU254
25781.3
0.88
0.169
5


LNU48
24804.4
10.81
0.194
4
LNU25
14082.8
1.07
0.473
6
LNU254
25782.5
0.87
0.508
4


LNU48
24802.1
10.75
0.502
3
LNU254
25781.3
1.09
0.263
8
LNU263
25791.3
1.03
0.308
23


LNU48
24801.4
10.70
0.320
3
LNU254
25782.5
1.07
0.363
6
LNU263
25794.8
1.01
0.074
20


LNU63
24814.2
11.13
0.149
7
LNU254
25782.4
1.06
0.671
5
LNU267
25804.3
0.89
0.244
7


LNU63
24814.7
10.94
0.297
5
LNU263
25791.3
1.25
0.376
23
LNU271
25913.3
0.93
0.373
11


LNU7
25081.1
11.56
0.271
11
LNU263
25794.8
1.22
0.078
21
LNU278
25812.3
0.94
0.232
12


LNU8
25063.1
11.38
0.098
10
LNU267
25804.3
1.03
0.799
2
LNU278
25814.1
0.88
0.251
4


LNU8
25062.1
11.23
0.337
8
LNU271
25913.3
1.10
0.642
9
LNU36
25562.3
0.90
0.540
7


LNU8
25061.2
11.06
0.001
6
LNU271
25912.1
1.05
0.719
4
LNU36
25562.9
0.87
0.797
4


LNU8
25062.2
10.81
0.586
4
LNU278
25814.3
1.16
0.190
15
LNU36
25562.7
0.87
0.704
3


LNU94
24833.3
11.50
0.252
11
LNU278
25812.3
1.07
0.388
6
LNU43
14421.1
0.99
0.255
18


LNU94
24834.4
11.13
0.149
7
LNU36
25562.3
1.15
0.107
14
LNU43
14422.8
0.96
0.014
15


LNU94
24831.4
10.88
0.401
5
LNU43
14423.6
1.21
0.313
20
LNU43
14423.6
0.93
0.587
11


LNU94
24833.1
10.81
0.381
4
LNU43
14422.8
1.13
0.122
12
LNU43
14423.7
0.88
0.607
5


LNU96
25071.2
11.44
0.141
10
LNU43
14421.1
1.11
0.465
10
LNU45
25053.4
0.93
0.154
11


LNU96
25071.3
11.19
0.299
8
LNU43
14422.9
1.08
0.337
7
LNU45
25052.9
0.86
0.458
3


LNU96
25073.4
11.13
0.149
7
LNU45
25053.4
1.22
0.027
21
LNU67
25824.5
1.02
0.168
21


CONT.
—
10.00
—
0
LNU67
25824.5
1.29
0.024
28
LNU67
25821.5
0.97
0.324
16


LNU122
25332.5
10.88
0.388
9
LNU67
25821.5
1.27
0.200
26
LNU67
25823.5
0.91
0.481
9


LNU122
25332.2
10.63
0.323
6
LNU67
25823.5
1.06
0.563
5
LNU67
25821.4
0.90
0.545
7


LNU125
25944.1
10.69
0.089
7
CONT.
—
0.78
—
0
CONTROL
—
0.82
—
0


LNU125
25941.4
10.56
0.292
6
LNU100
14472.1
0.96
0.137
23
LNU100
14473.1
0.98
0.000
18


LNU125
25943.2
10.31
0.297
3
LNU100
14473.3
0.92
0.300
19
LNU100
14472.1
0.94
0.041
15


LNU178
14611.5
10.31
0.603
3
LNU100
14473.1
0.92
0.061
18
LNU100
14473.3
0.86
0.298
5


LNU236
25425.3
10.50
0.003
5
LNU104
25032.2
1.05
0.002
35
LNU104
25032.2
1.02
0.179
24


LNU236
25422.4
10.31
0.053
3
LNU104
25033.3
0.97
0.085
24
LNU104
25033.3
1.00
0.144
21


LNU236
25424.2
10.25
0.488
3
LNU104
25032.1
0.84
0.203
8
LNU104
25032.1
0.87
0.339
6


LNU25
14082.8
10.44
0.493
4
LNU106
14483.2
0.94
0.396
21
LNU106
14481.1
0.92
0.716
11


LNU271
25912.1
10.19
0.492
2
LNU106
14483.5
0.90
0.119
16
LNU106
14483.5
0.90
0.549
9


LNU278
25814.3
10.50
0.394
5
LNU106
14481.1
0.90
0.504
15
LNU106
14483.2
0.85
0.510
3


LNU278
25814.1
10.25
0.238
3
LNU106
14484.3
0.83
0.332
6
LNU106
14484.3
0.85
0.708
3


LNU45
25053.4
10.50
0.394
5
LNU114
25041.2
1.01
0.294
29
LNU114
25041.1
0.95
0.413
15


LNU45
25052.11
10.38
0.108
4
LNU114
25044.4
0.86
0.562
10
LNU114
25041.2
0.89
0.303
8


LNU45
25052.12
10.25
0.795
3
LNU114
25041.1
0.82
0.781
5
LNU117
25931.4
0.97
0.303
17


LNU67
25823.5
10.63
0.028
6
LNU114
25042.1
0.81
0.797
4
LNU117
25932.4
0.92
0.034
11


CONT.
—
11.19
—
0
LNU117
25931.4
0.96
0.082
23
LNU117
25931.2
0.85
0.452
3


LNU168
24753.5
11.75
0.638
5
LNU117
25931.1
0.86
0.211
11
LNU218
24781.6
0.94
0.521
15


LNU173
25451.1
11.69
0.034
4
LNU155
14523.5
0.83
0.659
7
LNU218
24781.4
0.92
0.006
12


LNU173
25451.2
11.50
0.417
3
LNU218
24781.4
0.95
0.000
22
LNU218
24781.1
0.85
0.709
4


LNU178
14611.1
12.00
0.002
7
LNU218
24781.6
0.85
0.545
9
LNU254
25782.4
0.87
0.129
6


LNU178
14611.5
11.94
0.185
7
LNU218
24781.1
0.81
0.390
5
LNU4
25133.3
0.94
0.351
15


LNU184
25393.1
11.56
0.425
3
LNU254
25782.4
0.86
0.005
11
LNU4
25134.3
0.85
0.490
4


LNU230
25412.2
11.75
0.194
5
LNU4
25133.3
0.88
0.002
13
LNU40
24792.1
1.00
0.002
22


LNU230
25413.1
11.63
0.282
4
LNU4
25134.3
0.84
0.020
8
LNU40
24794.4
0.93
0.513
13


LNU236
25425.4
11.63
0.666
4
LNU4
25134.2
0.82
0.704
5
LNU40
24794.3
0.84
0.528
2


LNU236
25423.3
11.44
0.263
2
LNU40
24792.1
1.04
0.308
34
LNU46
14462.1
0.92
0.101
12


LNU236
25424.2
11.44
0.263
2
LNU40
24794.4
0.86
0.426
11
LNU46
14462.5
0.92
0.485
12


LNU24
24971.2
11.81
0.239
6
LNU40
24792.2
0.82
0.640
5
LNU46
14463.2
0.89
0.140
8


LNU263
25794.8
11.44
0.578
2
LNU40
24794.3
0.81
0.695
4
LNU48
24801.4
0.96
0.263
17


LNU276
25431.1
12.13
0.282
8
LNU46
14462.5
1.05
0.329
35
LNU48
24802.1
0.90
0.388
10


LNU276
25433.3
12.00
0.002
7
LNU46
14462.1
0.97
0.001
24
LNU48
24804.4
0.86
0.455
4


LNU279
25481.3
11.44
0.420
2
LNU48
24801.4
0.95
0.000
22
LNU48
24802.2
0.85
0.800
3


LNU53
25674.6
11.63
0.103
4
LNU48
24802.1
0.92
0.437
18
LNU63
24814.6
0.93
0.402
13


LNU73
25751.9
11.63
0.103
4
LNU48
24803.2
0.81
0.631
3
LNU63
24814.7
0.86
0.545
4


LNU9
25001.2
11.50
0.417
3
LNU63
24814.2
0.93
0.009
20
LNU63
24814.2
0.86
0.533
4


LNU9
25001.7
11.50
0.417
3
LNU63
24814.6
0.88
0.064
13
LNU7
25081.1
0.92
0.467
12


LNU9
25001.3
11.44
0.578
2
LNU63
24812.2
0.80
0.703
3
LNU8
25063.1
0.91
0.182
11


CONT.
—
8.52
—
0
LNU63
24812.3
0.80
0.542
2
LNU8
25062.1
0.89
0.508
9


LNU131
14005.2
9.63
0.000
13
LNU7
25081.1
0.91
0.017
17
LNU8
25062.2
0.87
0.493
6


LNU131
14002.15
9.25
0.371
9
LNU7
25083.1
0.82
0.766
5
LNU8
25061.2
0.85
0.329
4


LNU131
14005.5
9.25
0.000
9
LNU7
25083.3
0.81
0.337
4
LNU94
24831.4
0.94
0.025
15


LNU135
26204.4
9.25
0.444
9
LNU8
25063.1
0.96
0.184
23
LNU94
24833.1
0.90
0.463
9


LNU135
26204.2
9.13
0.033
7
LNU8
25062.1
0.89
0.480
14
LNU94
24833.3
0.86
0.278
5


LNU135
26203.1
9.06
0.505
6
LNU8
25061.2
0.84
0.400
8
LNU96
25071.3
0.87
0.751
6


LNU135
26203.3
9.06
0.140
6
LNU8
25062.2
0.82
0.204
6
LNU96
25071.2
0.87
0.169
6


LNU135
26203.4
9.06
0.622
6
LNU94
24833.1
0.95
0.000
22
CONT.
—
0.83
—
0


LNU173
25451.2
9.56
0.417
12
LNU94
24833.3
0.94
0.127
21
LNU10
25123.5
0.90
0.591
8


LNU173
25451.5
9.13
0.202
7
LNU94
24831.4
0.94
0.036
21
LNU122
25332.5
0.93
0.021
11


LNU181
25774.1
9.44
0.000
11
LNU94
24834.4
0.90
0.624
15
LNU122
25332.2
0.88
0.460
6


LNU181
25771.6
9.31
0.076
9
LNU96
25071.2
0.86
0.223
10
LNU122
25332.1
0.85
0.615
3


LNU181
25771.8
8.88
0.118
4
LNU96
25073.4
0.86
0.195
10
LNU125
25941.4
0.92
0.309
11


LNU181
25771.5
8.81
0.508
3
LNU96
25071.3
0.82
0.753
5
LNU178
14611.1
0.86
0.609
3


LNU181
25771.2
8.69
0.762
2
CONT.
—
0.80
—
0
LNU234
25014.4
0.93
0.115
12


LNU184
25393.2
9.56
0.000
12
LNU10
25123.5
0.95
0.358
19
LNU236
25425.3
0.92
0.298
11


LNU184
25394.1
9.13
0.202
7
LNU122
25332.5
1.04
0.000
30
LNU236
25425.4
0.89
0.516
8


LNU184
25393.1
9.06
0.140
6
LNU122
25332.2
0.93
0.177
17
LNU236
25423.3
0.86
0.230
3


LNU184
25394.3
9.06
0.420
6
LNU122
25332.1
0.88
0.010
10
LNU25
14082.8
0.93
0.045
12


LNU184
25393.3
9.04
0.334
6
LNU125
25941.4
0.91
0.015
14
LNU25
14083.7
0.91
0.407
9


LNU184
25395.1
9.00
0.407
6
LNU125
25943.2
0.87
0.021
10
LNU267
25803.1
0.87
0.095
5


LNU224
25872.3
9.75
0.080
14
LNU178
14611.5
0.94
0.197
18
LNU271
25913.3
0.91
0.307
10


LNU224
25874.1
9.69
0.131
14
LNU178
14611.1
0.89
0.325
12
LNU271
25911.4
0.85
0.380
3


LNU224
25874.4
9.56
0.150
12
LNU178
14612.1
0.85
0.692
6
LNU278
25814.1
0.93
0.001
12


LNU224
25872.2
9.00
0.678
6
LNU220
25405.1
0.82
0.402
3
LNU278
25814.3
0.88
0.054
6


LNU224
25871.3
8.88
0.118
4
LNU234
25014.5
0.91
0.519
14
LNU278
25812.2
0.86
0.687
4


LNU246
25744.2
9.69
0.131
14
LNU234
25014.4
0.89
0.324
12
LNU43
14423.7
0.86
0.635
3


LNU246
25744.3
9.69
0.039
14
LNU234
25014.6
0.88
0.716
11
LNU45
25052.12
0.95
0.351
15


LNU246
25744.4
9.13
0.505
7
LNU236
25423.3
0.87
0.160
10
LNU45
25053.4
0.92
0.098
10


LNU246
25743.2
8.81
0.508
3
LNU236
25424.2
0.85
0.481
7
LNU45
25052.11
0.86
0.741
3


LNU246
25743.1
8.77
0.634
3
LNU236
25425.3
0.84
0.790
5
LNU67
25823.5
0.96
0.000
15


LNU250
25591.1
9.44
0.174
11
LNU236
25425.4
0.83
0.385
5
LNU9
25001.1
0.88
0.507
7


LNU250
25592.2
8.94
0.388
5
LNU25
14083.7
0.93
0.119
17
LNU9
25001.7
0.85
0.430
2


LNU250
25591.3
8.88
0.118
4
LNU25
14082.8
0.85
0.075
7
CONT.
—
0.91
—
0


LNU260
26404.1
9.44
0.059
11
LNU267
25803.1
0.83
0.236
4
LNU10
25123.6
0.99
0.534
9


LNU260
26403.1
8.88
0.007
4
LNU271
25911.4
0.92
0.001
16
LNU157
24982.7
0.96
0.550
5


LNU260
26404.7
8.81
0.317
3
LNU271
25913.3
0.82
0.358
3
LNU168
24753.5
0.98
0.046
8


LNU276
25433.6
9.56
0.304
12
LNU271
25912.1
0.81
0.607
2
LNU173
25451.1
1.04
0.071
14


LNU276
25433.3
9.19
0.247
8
LNU278
25814.3
0.98
0.000
23
LNU178
14611.4
1.02
0.358
12


LNU276
25433.5
9.00
0.407
6
LNU278
25814.1
0.97
0.000
22
LNU178
14611.5
1.00
0.524
9


LNU276
25431.1
8.75
0.511
3
LNU278
25812.3
0.89
0.147
12
LNU178
14611.1
0.98
0.049
7


LNU279
25484.3
9.38
0.131
10
LNU278
25813.2
0.88
0.659
10
LNU184
25395.1
1.04
0.002
14


LNU279
25481.3
9.19
0.000
8
LNU278
25812.2
0.85
0.696
6
LNU184
25393.1
1.03
0.381
13


LNU279
25481.2
8.88
0.118
4
LNU43
14423.6
0.89
0.316
12
LNU184
25394.3
1.03
0.335
13


LNU279
25481.4
8.69
0.680
2
LNU43
14423.7
0.85
0.376
7
LNU184
25393.2
1.01
0.354
11


LNU3
26124.3
9.06
0.002
6
LNU45
25052.12
1.00
0.263
26
LNU230
25413.2
1.06
0.291
17


LNU3
26122.2
8.75
0.643
3
LNU45
25053.4
0.97
0.179
21
LNU230
25415.1
1.05
0.005
16


LNU33
25552.2
9.25
0.020
9
LNU45
25052.9
0.88
0.137
10
LNU230
25412.2
1.02
0.344
12


LNU33
25553.3
9.19
0.355
8
LNU45
25052.11
0.86
0.590
8
LNU230
25413.1
1.00
0.012
10


LNU53
25674.5
8.69
0.214
2
LNU67
25823.5
1.10
0.003
38
LNU230
25412.1
0.93
0.714
2


LNU53
25674.6
8.69
0.762
2
LNU67
25824.3
0.83
0.751
5
LNU236
25424.2
1.06
0.447
16


CONT.
—
10.18
—
0
LNU67
25824.5
0.82
0.693
3
LNU236
25425.4
1.03
0.545
13


LNU119
26142.8
10.44
0.112
3
LNU67
25821.5
0.82
0.558
3
LNU236
25423.3
1.01
0.324
11


LNU130
24913.6
10.75
0.206
6
LNU9
25001.7
0.89
0.264
12
LNU24
24971.2
1.08
0.157
19


LNU130
24912.7
10.56
0.222
4
LNU9
25003.1
0.85
0.350
6
LNU24
24974.2
0.99
0.440
9


LNU130
24911.7
10.38
0.567
2
LNU9
25001.3
0.82
0.418
3
LNU24
24971.3
0.97
0.303
6


LNU136
14515.1
11.06
0.051
9
CONT.
—
1.00
—
0
LNU24
24971.4
0.93
0.796
2


LNU142
27541.1
10.63
0.009
4
LNU10
25123.6
1.30
0.206
30
LNU263
25794.8
1.04
0.342
14


LNU142
27545.1
10.50
0.632
3
LNU10
25123.5
1.16
0.000
16
LNU276
25433.3
1.00
0.036
9


LNU142
27546.2
10.44
0.363
3
LNU157
24982.8
1.11
0.005
11
LNU276
25431.1
0.93
0.716
2


LNU149
26175.1
10.88
0.458
7
LNU157
24982.4
1.09
0.165
9
LNU279
25481.3
0.98
0.043
8


LNU149
26175.7
10.69
0.143
5
LNU168
24753.5
1.05
0.467
5
LNU279
25481.4
0.96
0.480
6


LNU15
14123.11
10.44
0.363
3
LNU173
25451.1
1.08
0.026
8
LNU36
25562.3
1.00
0.294
10


LNU185
26474.2
11.00
0.242
8
LNU173
25451.2
1.05
0.537
5
LNU53
25674.1
1.08
0.187
18


LNU185
26475.1
10.38
0.567
2
LNU178
14611.5
1.20
0.032
20
LNU53
25674.2
1.06
0.073
16


LNU212
25834.1
10.75
0.002
6
LNU178
14611.4
1.19
0.217
19
LNU53
25674.6
0.96
0.197
5


LNU212
25834.5
10.69
0.007
5
LNU178
14611.1
1.18
0.069
18
LNU56
24694.1
1.01
0.043
11


LNU212
25834.4
10.37
0.772
2
LNU184
25393.2
1.28
0.000
28
LNU56
24694.2
0.98
0.420
8


LNU212
25832.1
10.35
0.224
2
LNU184
25393.1
1.25
0.468
25
LNU56
24693.1
0.98
0.048
7


LNU216
25982.2
10.69
0.439
5
LNU184
25394.3
1.21
0.440
21
LNU56
24693.2
0.96
0.202
6


LNU216
25984.6
10.69
0.143
5
LNU184
25395.1
1.19
0.089
19
LNU73
25751.1
1.06
0.531
16


LNU216
25985.4
10.50
0.539
3
LNU184
25393.3
1.02
0.563
2
LNU73
25755.1
1.04
0.223
14


LNU216
25982.1
10.44
0.363
3
LNU230
25415.1
1.34
0.109
34
LNU73
25754.2
1.00
0.624
9


LNU228
26224.7
10.81
0.002
6
LNU230
25412.1
1.18
0.043
18
LNU73
25751.9
0.99
0.205
8


LNU228
26222.4
10.56
0.222
4
LNU230
25413.2
1.17
0.119
17
LNU73
25751.8
0.97
0.778
6


LNU228
26224.6
10.44
0.549
3
LNU230
25413.1
1.15
0.021
15
LNU9
25001.7
1.07
0.049
17


LNU274
26265.1
10.44
0.112
3
LNU230
25412.2
1.13
0.020
13
LNU9
25001.2
1.03
0.562
13


LNU274
26262.2
10.38
0.689
2
LNU236
25425.4
1.25
0.002
25
LNU9
25001.1
1.00
0.008
10


LNU277
25845.1
10.56
0.413
4
LNU236
25424.2
1.22
0.064
23
LNU9
25001.3
0.97
0.625
6


LNU280
26164.2
10.88
0.001
7
LNU236
25422.4
1.17
0.000
17
LNU9
25003.1
0.96
0.591
5


LNU280
26164.4
10.69
0.591
5
LNU236
25423.3
1.17
0.372
17
CONT.
—
0.60
—
0


LNU280
26162.1
10.56
0.413
4
LNU24
24974.2
1.19
0.247
20
LNU131
14005.2
0.87
0.000
45


LNU55
26015.3
10.56
0.028
4
LNU24
24971.3
1.16
0.500
16
LNU131
14005.5
0.78
0.003
29


LNU81
26034.2
10.50
0.632
3
LNU24
24971.2
1.10
0.172
10
LNU135
26203.1
0.78
0.594
29


LNU81
26034.3
10.50
0.632
3
LNU24
24972.1
1.07
0.056
7
LNU135
26204.2
0.70
0.288
16


CONT.
—
10.58
—
0
LNU24
24971.4
1.03
0.781
3
LNU135
26203.4
0.68
0.746
13


LNU119
26142.8
10.81
0.330
2
LNU263
25794.8
1.28
0.169
28
LNU135
26203.3
0.62
0.559
3


LNU130
24913.5
11.44
0.040
8
LNU263
25791.3
1.11
0.713
11
LNU161
14552.9
0.70
0.356
16


LNU136
14511.10
11.19
0.032
6
LNU276
25433.3
1.28
0.035
29
LNU173
25451.2
0.72
0.605
19


LNU136
14515.1
10.88
0.444
3
LNU276
25431.1
1.03
0.504
3
LNU181
25774.1
0.76
0.002
26


LNU142
27545.1
11.38
0.106
7
LNU279
25481.3
1.24
0.341
24
LNU181
25771.8
0.76
0.085
26


LNU142
27541.1
11.19
0.100
6
LNU279
25481.5
1.20
0.322
20
LNU181
25771.6
0.69
0.368
14


LNU212
25834.5
11.44
0.262
8
LNU279
25481.4
1.18
0.055
18
LNU181
25771.5
0.66
0.207
9


LNU212
25833.2
11.19
0.249
6
LNU279
25484.3
1.05
0.606
5
LNU184
25393.1
0.79
0.182
31


LNU216
25984.6
11.06
0.165
5
LNU36
25562.3
1.06
0.391
6
LNU184
25393.3
0.79
0.416
30


LNU216
25982.2
10.88
0.444
3
LNU36
25562.7
1.05
0.251
5
LNU184
25394.1
0.74
0.234
23


LNU216
25984.1
10.81
0.459
2
LNU53
25674.1
1.30
0.163
30
LNU184
25394.3
0.74
0.498
23


LNU228
26222.1
11.00
0.302
4
LNU53
25674.2
1.07
0.456
7
LNU184
25393.2
0.71
0.003
18


LNU228
26224.7
10.94
0.276
3
LNU53
25674.6
1.07
0.042
7
LNU184
25395.1
0.65
0.404
8


LNU274
26264.2
11.06
0.165
5
LNU56
24693.1
1.14
0.252
14
LNU224
25872.3
0.93
0.003
54


LNU277
25842.3
10.88
0.275
3
LNU56
24694.1
1.13
0.018
13
LNU224
25874.4
0.82
0.142
36


LNU280
26164.4
11.00
0.099
4
LNU56
24693.2
1.08
0.395
8
LNU224
25874.1
0.77
0.387
28


LNU55
26013.9
10.81
0.756
2
LNU73
25755.1
1.22
0.115
22
LNU224
25872.2
0.77
0.403
27


LNU81
26034.2
11.00
0.552
4
LNU73
25751.1
1.19
0.593
19
LNU224
25871.3
0.63
0.558
4


LNU81
26031.10
10.88
0.665
3
LNU73
25754.2
1.16
0.492
16
LNU246
25744.2
0.79
0.201
32


LNU81
26034.3
10.88
0.578
3
LNU73
25751.8
1.14
0.457
14
LNU246
25744.3
0.78
0.375
29







LNU73
25751.9
1.09
0.180
9
LNU246
25744.4
0.77
0.608
27







LNU9
25001.1
1.30
0.010
30
LNU250
25591.1
0.90
0.167
49







LNU9
25001.7
1.19
0.260
19
LNU250
25592.2
0.81
0.002
34







LNU9
25001.3
1.16
0.365
16
LNU260
26404.1
0.66
0.499
10







LNU9
25001.2
1.14
0.394
14
LNU260
26403.1
0.64
0.234
6







CONT.
—
0.86
—
0
LNU276
25433.6
0.83
0.201
37







LNU131
14005.2
1.26
0.000
46
LNU276
25433.5
0.74
0.245
22







LNU131
14005.5
1.13
0.000
31
LNU279
25481.3
0.86
0.338
43







LNU135
26203.4
0.96
0.768
11
LNU279
25484.3
0.71
0.486
17







LNU135
26204.2
0.93
0.333
8
LNU279
25481.5
0.69
0.577
14







LNU173
25451.2
0.99
0.530
15
LNU3
26123.5
0.71
0.515
18







LNU181
25771.8
1.10
0.417
27
LNU3
26124.1
0.70
0.779
15







LNU181
25771.5
1.00
0.513
16
LNU3
26124.3
0.67
0.035
12







LNU181
25771.6
1.00
0.014
16
LNU3
26123.6
0.66
0.533
9







LNU181
25774.1
0.94
0.123
9
LNU3
26122.2
0.65
0.194
8







LNU184
25393.2
1.11
0.000
29
LNU33
25552.2
0.67
0.463
11







LNU184
25394.1
1.07
0.293
24
LNU33
25553.3
0.66
0.224
10







LNU184
25393.1
0.99
0.197
15
LNU53
25674.5
0.72
0.129
19







LNU184
25393.3
0.99
0.655
15
LNU53
25674.1
0.68
0.641
13







LNU184
25394.3
0.95
0.736
11
LNU53
25674.6
0.63
0.661
4







LNU224
25872.3
1.26
0.036
46
LNU56
24694.2
0.70
0.010
16







LNU224
25874.4
1.11
0.110
29
CONT.
—
0.88
—
0







LNU224
25874.1
1.11
0.416
29
LNU130
24912.7
1.07
0.223
21







LNU224
25872.2
1.08
0.309
25
LNU130
24911.7
1.00
0.492
13







LNU246
25744.4
1.13
0.553
31
LNU130
24913.6
0.95
0.430
8







LNU246
25744.3
1.13
0.587
31
LNU130
24914.5
0.90
0.785
3







LNU246
25744.2
1.10
0.259
28
LNU136
14515.1
1.01
0.272
14







LNU246
25743.1
1.06
0.214
23
LNU142
27541.1
0.96
0.031
9







LNU246
25743.2
0.95
0.095
11
LNU149
26175.1
0.92
0.662
5







LNU250
25591.1
1.23
0.129
43
LNU15
14123.11
1.01
0.138
15







LNU250
25592.2
1.01
0.010
17
LNU15
14122.9
0.95
0.466
8







LNU260
26404.1
0.91
0.655
5
LNU15
14122.8
0.94
0.149
7







LNU276
25433.6
1.12
0.280
30
LNU15
14123.13
0.93
0.183
6







LNU276
25433.5
0.96
0.621
12
LNU185
26475.1
1.03
0.395
18







LNU279
25481.3
1.19
0.435
38
LNU185
26472.1
0.99
0.436
13







LNU279
25484.3
1.16
0.212
34
LNU185
26474.2
0.98
0.028
11







LNU279
25481.5
0.97
0.605
12
LNU212
25834.5
0.98
0.025
12







LNU279
25481.2
0.96
0.279
12
LNU212
25834.1
0.96
0.391
10







LNU33
25553.3
0.96
0.576
11
LNU216
25982.1
1.01
0.001
15







LNU53
25674.5
0.92
0.285
7
LNU216
25985.4
0.97
0.557
10







LNU56
24694.2
0.93
0.292
8
LNU216
25984.1
0.91
0.292
4







CONT.
—
1.03
—
0
LNU216
25984.6
0.90
0.693
2







LNU119
26142.8
1.07
0.559
4
LNU228
26222.4
1.10
0.057
25







LNU130
24912.7
1.26
0.102
23
LNU228
26222.1
0.95
0.291
8







LNU130
24913.5
1.14
0.374
11
LNU228
26224.6
0.93
0.423
6







LNU130
24911.7
1.11
0.732
7
LNU229
26112.3
0.96
0.264
9







LNU130
24913.6
1.07
0.672
4
LNU229
26111.7
0.95
0.079
8







LNU136
14515.1
1.18
0.067
15
LNU241
26232.4
0.99
0.432
12







LNU142
27541.1
1.13
0.533
10
LNU241
26233.2
0.92
0.441
5







LNU142
27545.1
1.10
0.737
6
LNU253
26243.3
0.90
0.536
2







LNU15
14123.13
1.15
0.362
11
LNU274
26262.2
0.95
0.487
8







LNU15
14123.11
1.11
0.073
8
LNU280
26162.1
1.04
0.456
18







LNU185
26475.1
1.18
0.387
15
LNU280
26162.7
0.96
0.248
9







LNU185
26474.2
1.07
0.388
4
LNU280
26164.4
0.95
0.713
7







LNU212
25834.5
1.20
0.002
16
LNU55
26015.1
1.05
0.364
19







LNU212
25834.1
1.11
0.719
8
LNU55
26015.3
0.92
0.303
5







LNU216
25985.4
1.17
0.706
13
LNU81
26034.3
1.01
0.003
14







LNU216
25982.1
1.10
0.527
6
CONT.
—
0.94
—
0







LNU228
26224.7
1.09
0.568
6
LNU119
26142.8
1.05
0.360
12







LNU228
26222.4
1.09
0.344
6
LNU119
26144.2
1.03
0.028
9







LNU241
26233.2
1.11
0.160
8
LNU119
26141.1
1.03
0.219
9







LNU241
26232.
1.06
0.592
3
LNU130
24913.5
1.06
0.379
12







LNU274
26262.2
1.08
0.753
5
LNU130
24914.5
1.03
0.141
10







LNU274
26265.1
1.06
0.397
3
LNU136
14515.1
1.06
0.310
13







LNU277
25845.1
1.18
0.363
15
LNU142
27541.2
1.06
0.366
13







LNU280
26162.1
1.23
0.563
20
LNU142
27541.1
1.03
0.521
10







LNU280
26162.7
1.15
0.086
12
LNU142
27545.1
1.03
0.100
9







LNU55
26015.1
1.12
0.158
9
LNU149
26174.7
1.05
0.622
12







LNU55
26015.3
1.05
0.540
2
LNU15
14122.8
1.20
0.478
28







LNU81
26034.3
1.26
0.001
22
LNU15
14123.11
1.12
0.500
19







CONT.
—
1.10
—
0
LNU15
14123.13
1.05
0.263
12







LNU119
26142.8
1.27
0.139
16
LNU15
14122.9
0.99
0.563
5







LNU119
26141.1
1.27
0.565
15
LNU185
26472.1
1.04
0.507
11







LNU130
24914.5
1.32
0.004
20
LNU185
26474.2
0.98
0.645
4







LNU136
14515.1
1.21
0.274
10
LNU185
26474.1
0.96
0.547
2







LNU142
27541.1
1.21
0.638
10
LNU212
25834.5
1.05
0.388
11







LNU142
27541.2
1.17
0.098
6
LNU212
25834.1
1.04
0.612
11







LNU15
14123.11
1.42
0.327
29
LNU212
25833.2
1.04
0.551
11







LNU15
14122.8
1.36
0.570
24
LNU212
25834.4
1.00
0.545
6







LNU15
14123.13
1.25
0.308
13
LNU216
25984.6
1.09
0.113
16







LNU185
26474.1
1.20
0.433
9
LNU216
25984.1
1.05
0.100
12







LNU212
25834.5
1.24
0.411
13
LNU216
25982.2
0.99
0.087
6







LNU212
25834.4
1.18
0.605
7
LNU216
25982.1
0.98
0.148
5







LNU212
25834.1
1.17
0.479
6
LNU228
26224.7
1.17
0.050
25







LNU212
25833.2
1.15
0.765
4
LNU228
26224.6
1.04
0.620
11







LNU216
25984.1
1.19
0.587
8
LNU228
26222.1
1.04
0.028
11







LNU216
25982.2
1.16
0.031
6
LNU228
26225.2
0.99
0.120
5







LNU216
25984.6
1.14
0.675
4
LNU229
26111.5
1.00
0.301
6







LNU228
26224.7
1.33
0.012
21
LNU241
26232.4
1.06
0.094
13







LNU228
26222.1
1.30
0.015
18
LNU253
26243.3
1.03
0.292
9







LNU228
26225.2
1.14
0.627
3
LNU274
26262.2
1.00
0.069
6







LNU229
26111.5
1.27
0.000
15
LNU277
25842.3
1.09
0.283
16







LNU229
26112.4
1.13
0.720
3
LNU277
25844.3
0.98
0.547
5







LNU241
26232.4
1.30
0.340
18
LNU280
26164.3
1.08
0.014
15







LNU253
26242.1
1.17
0.621
6
LNU280
26162.7
1.06
0.452
13







LNU274
26262.2
1.15
0.489
5
LNU280
26164.4
1.04
0.114
11







LNU277
25842.3
1.24
0.486
13
LNU280
26162.1
1.04
0.496
11







LNU280
26164.4
1.25
0.101
13
LNU55
26013.4
0.96
0.409
3







LNU280
26162.1
1.18
0.024
7
LNU81
26031.10
1.14
0.307
21







LNU81
26031.10
1.38
0.204
25
LNU81
26034.3
1.11
0.438
18







LNU81
26031.9
1.17
0.607
6
LNU81
26034.2
1.05
0.021
12












LNU81
26031.9
0.97
0.711
3





Table 84.


“CONT.”—Control;


“Ave.”—Average;


“% Incr.” = % increment.






Although the invention has been described in conjunction with specific embodiments thereof, it is evident that many alternatives, modifications and variations will be apparent to those skilled in the art. Accordingly, it is intended to embrace all such alternatives, modifications and variations that fall within the spirit and broad scope of the appended claims.
All publications, patents and patent applications mentioned in this specification are herein incorporated in their entirety by reference into the specification, to the same extent as if each individual publication, patent or patent application was specifically and individually indicated to be incorporated herein by reference. In addition, citation or identification of any reference in this application shall not be construed as an admission that such reference is available as prior art to the present invention. To the extent that section headings are used, they should not be construed as necessarily limiting.

## Claims

1. A method of increasing nitrogen use efficiency, yield, biomass, growth rate, vigor, and/or tolerance to nitrogen deficiency of a plant, comprising expressing within the plant an exogenous polynucleotide comprising the nucleic acid sequence set forth in SEQ ID NO:420, thereby increasing the nitrogen use efficiency, yield, biomass, growth rate, vigor, and/or tolerance to nitrogen deficiency of the plant.

2. A method of increasing nitrogen use efficiency, yield, biomass, growth rate, vigor, and/or tolerance to nitrogen deficiency of a plant, comprising expressing within the plant an exogenous polynucleotide comprising a nucleic acid sequence encoding the polypeptide set forth in SEQ ID NO: 634, thereby increasing the nitrogen use efficiency, yield, biomass, growth rate, vigor, and/or tolerance to nitrogen deficiency of the plant.

3. The method of claim 1, wherein the yield comprises seed yield or oil yield.

4. The method of claim 1, further comprising growing the plant expressing said exogenous polynucleotide under nitrogen-limiting conditions.

5. The method of claim 2, wherein said nucleic acid sequence is set forth by SEQ ID NO: 167.

6. The method of claim 1, further comprising selecting a plant expressing said exogenous polynucleotide for an increased biomass as compared to a non-transformed plant grown under the same growth conditions.

7. The method of claim 1, further comprising selecting a plant expressing said exogenous polynucleotide for an increased yield as compared to a non-transformed plant grown under the same growth conditions.

8. The method of claim 1, further comprising selecting a plant expressing said exogenous polynucleotide for an increased nitrogen use efficiency as compared to a non-transformed plant grown under the same growth conditions.

9. The method of claim 1, further comprising selecting a plant expressing said exogenous polynucleotide for an increased growth rate as compared to a non-transformed plant grown under the same growth conditions.

10. The method of claim 1, further comprising selecting a plant expressing said exogenous polynucleotide for an increased tolerance to nitrogen deficiency as compared to a non-transformed plant grown under the same growth conditions.

11. The method of claim 2, further comprising selecting a plant expressing said exogenous polynucleotide for an increased biomass as compared to a non-transformed plant grown under the same growth conditions.

12. The method of claim 2, further comprising selecting a plant expressing said exogenous polynucleotide for an increased yield as compared to a non-transformed plant grown under the same growth conditions.

13. The method of claim 2, further comprising selecting a plant expressing said exogenous polynucleotide for an increased nitrogen use efficiency as compared to a non-transformed plant grown under the same growth conditions.

14. The method of claim 2, further comprising selecting a plant expressing said exogenous polynucleotide for an increased growth rate as compared to a non-transformed plant grown under the same growth conditions.

15. The method of claim 2, further comprising selecting a plant expressing said exogenous polynucleotide for an increased tolerance to nitrogen deficiency as compared to a non-transformed plant grown under the same growth conditions.