Triflusulfuron-methyl (129002) Summary of Analytical Chemistry and Residue Data Barcode: D388620

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UNITED STATES ENVIRONMENTAL PROTECTION AGENCY

WASHINGTON, D.C. 20460

OFFICE OF CHEMICAL SAFETY AND

 POLLUTION PREVENTION

MEMORANDUM

Date: April 13, 2011

Subject: Triflusulfuron-methyl. Petition for the Establishment of Permanent Tolerances and 

Registration for Use on Garden Beets (PP 9E7669). Summary of Analytical 

Chemistry and Residue Data.

PC Code: 129002 DP Barcode: D388620

Decision No.: 425459 Registration No.: 352-569

Petition No.: 9E7669 Regulatory Action: Section 3

Assessment Type: Single Chemical Registration Case No.: NA

TXR No.: None CAS No.: 126535-15-7

MRID No.: 47943201 40 CFR §180.492

FROM: Elizabeth Holman, Chemist

Risk Assessment Branch II 

Health Effects Division (7509P)

THROUGH: William T. Drew, Chemist

 Risk Assessment Branch II (RAB2)

Health Effects Division (HED) (7509P)

 and

Richard A. Loranger, Ph.D., Senior Scientist

Risk Assessment Branch II

Health Effects Division (7509P)

TO: Laura Nollen/Barbara Madden 

Minor Use Team

Registration Division (7505P)

The residue chemistry summary document completed 2/17/11 (D376350; E. Holman) has been 

revised to address an issue related to plant-back intervals for rotational crops. 
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Table of Contents

Executive Summary ...................................................................................................................... 3

Residue Chemistry Deficiencies................................................................................................... 4

Background ................................................................................................................................... 5

860.1200 Directions for Use......................................................................................................... 6

860.1300 Nature of the Residue - Plants ..................................................................................... 8

860.1300 Nature of the Residue - Livestock................................................................................ 8

860.1340 Residue Analytical Methods......................................................................................... 9

860.1360 Multiresidue Methods................................................................................................. 11

860.1380 Storage Stability.......................................................................................................... 11

860.1400 Water, Fish, and Irrigated Crops.............................................................................. 11

860.1460 Food Handling............................................................................................................. 11

860.1480 Meat, Milk, Poultry, and Eggs................................................................................... 12

860.1500 Crop Field Trials......................................................................................................... 12

860.1520 Processed Food and Feed ........................................................................................... 16

860.1650 Submittal of Analytical Reference Standards.......................................................... 18

860.1850 Confined Accumulation in Rotational Crops........................................................... 17

860.1900 Field Accumulation in Rotational Crops.................................................................. 18

860.1550 Proposed Tolerances................................................................................................... 18
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Executive Summary

Triflusulfuron-methyl is a sulfonylurea herbicide that inhibits the biosynthesis of branched-chain 

amino acids in plants (via inhibition of the plant enzyme acetolactate synthase). Marketed as 

UpBeet, a dry flowable (DF) formulation containing 50% active ingredient (ai), triflusulfuronmethyl is currently approved for use on sugar beet commodities and chicory using broadcast 

(ground- or aerial-based) or band application methods at seasonal rates of up to 0.078 pounds ai 

per acre (lb ai/A). UpBeet is not to be applied via any type of irrigation system. The existing 

label for UpBeet lists a pre-harvest interval (PHI) of 60 days for both sugar beet and chicory. 

The registrant, E. I. du Pont de Nemours and Company, has submitted a supplemental label for a 

proposed use on garden beets. 

The maximum proposed single application rate on this supplemental label is 0.0156 lb ai/A for 

agricultural use on garden beets for control of broadleaf weeds, with a maximum seasonal 

application rate of 0.047 lb ai/A. Consistent with the existing uses, the requested new use on 

garden beet involves aerial or groundboom application methods. The proposed PHI on garden 

beet is 30 days, a value that is well supported by the garden beet field trial data submitted for this 

new use petition. 

The residue chemistry database is relatively complete and there are no residue chemistry issues 

that would preclude granting the requested new use and establishing permanent tolerances on 

garden beet roots and tops. The petitioner has previously submitted data depicting the nature of 

the residue in sugar beet and lactating goat. For the purposes of this petition on garden beet, the 

previously reviewed sugar beet metabolism study is sufficient to support the requested use of 

triflusulfuron-methyl on garden beets. The proposed tolerance expression in/on garden beets 

should include only the parent compound, triflusulfuron-methyl. The registrant is petitioning for 

tolerances of 0.01 ppm on garden beet roots and 0.02 ppm on garden beet tops. The existing 

tolerances on sugar beet and chicory commodities are 0.05 ppm. 

HED previously reviewed the goat metabolism study and cited deficiencies to be addressed 

should additional Section 3 registrations be pursued by the petitioner. However, based on residue 

levels observed in the goat metabolism study, finite residues are not expected in ruminant meat, 

meat byproduct, fat, or milk and tolerances are not required for these commodities. Therefore, 

the deficiencies cited by HED do not need to be addressed for the purposes of this new food use 

on garden beet. However, if future uses on large-acreage crops such as wheat, corn, or soybean 

are proposed, and consequently involve feedstuffs, then an adequate ruminant metabolism study

should be provided.

Adequate crop field trials and processing studies have been conducted to support tolerances in 

the plant commodities of sugar beet, chicory, and garden beet. Even at exaggerated rates (8X) 

for the field trial used to generate the processing study samples, no triflusulfuron-methyl was 

found in the sugar beet roots or processed commodities. DuPont Method 4480 was used to 

quantitate triflusulfuron-methyl residues in samples from the garden beet field trials currently

under review via solvent extraction and High Performance Liquid Chromatography-Mass 

Spectrometry/Mass Spectrometry (HPLC-MS/MS). With the exception of sugar beet tops at one 
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location that contained residues at the LOQ of 0.01 ppm, these eight field trials yielded residues 

that were less than the limit of quantitation. A registered non-ionic surfactant (NIS) or crop oil 

concentrate (COC) was added to the spray mixture for each application at a rate of 0.25% by

volume (v/v) at all garden beet field trials with the exception of a single trial conducted in 

Oregon. No impact on residue levels was observed in this non-adjuvant garden beet field trial. 

Therefore, adjuvant restrictions on the supplemental label are not needed. There are no 

processed commodities or livestock feed items associated with garden beet. The review of these 

garden beet field trials was originally conducted by Canada’s Pest Management Regulatory

Agency (PMRA) under a work share arrangement, but their conclusions have been reviewed by

HED and are consistent with OCSPP policy.

An analytical standard for triflusulfuron-methyl is currently available in the National Pesticide 

Standards Repository (NPSR). 

Residue Chemistry Deficiencies

The residue chemistry data set is complete with the exception of: 

1. The following label amendments are needed: a) The requested 30-day PHI for garden beet 

needs to be added to the supplemental label. b) The use of the aerial application method needs to 

be clarified in the supplemental label. The submitted supplemental label for garden beet does not 

include the petition-requested 30-day PHI, nor does it delineate the use of the aerial application 

method. Aerial application method instructions are included in the existing label for sugar beets.

2. Analytical Method – An adapted version of DuPont Method 4480 (MRID 48207101) was used 

in the submitted garden beet field trials under review in this petition. The enforcement method 

on record for triflusulfuron-methyl is method AMR 1930-91 (MRID 42496873), an HPLC-UV 

method. Method AMR 2021-91 (MRID 42496872) is very similar to AMR 1930-91, with the 

addition of an automatic switching column protocol, and was used in the previously submitted 

sugar beet and chicory field trials. Method 4480 is also similar to both these methods, with the 

primary difference being the addition of a tandem mass spectrometric detection protocol in Method 

4480. In addition, the independent laboratory validation (ILV) of Method 4480 successfully

demonstrated the reliability of the method on the first attempt. The registrant should clarify

whether they want to replace the existing enforcement method with Method 4480 when applying 

for future new uses, as this newer method does appear to be an acceptable enforcement method. 

Should the registrant decide to request Method 4480 be used for enforcement purposes, they will 

need to ensure that the standards are made available to the USEPA’s Analytical Chemistry Branch 

(ACB) in the event that they differ from those used in method AMR 1930-91. 

Regulatory Recommendations

There are no major deficiencies which would preclude the establishment of permanent tolerances 

for the use of triflusulfuron-methyl (as the DF formulation, UpBeet) on garden beets. Provided 
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that the forthcoming human health risk assessment (D373433; E.Holman; January 2011) does not 

identify any issues of concern, the submitted data support tolerances for residues of 

triflusulfuron-methyl, including its metabolites and degradates, in or on garden beet roots and 

tops, at the levels listed below. Compliance with the tolerance levels specified below is to be 

determined by measuring triflusulfuron-methyl only. 

Beet, garden, roots ...............................................................0.01 ppm

Beet, garden, tops.................................................................0.02 ppm

Note to RD: According to HED’s Interim Guidance on Tolerance Expressions (S. Knizner, 27 

May 2009), the tolerance expression for triflusulfuron-methyl cited in 40CFR §180.492[a] should

be revised to state: 

Tolerances are established for residues of triflusulfuron-methyl, including its 

metabolites and degradates, in or on the commodities listed in the table 

below. Compliance with the tolerance levels specified below is to be 

determined by measuring only triflusulfuron-methyl (methyl 2-[[[[[4-

(dimethylamino)-6-(2,2,2-trifluoroethoxy)-1,3,5-triazin-2-

yl]amino]carbonyl]amino]sulfonyl]-3-methylbenzoate) in or on the 

commodity. 

Background

Triflusulfuron-methyl is a post-emergence selective herbicide which acts by inhibiting 

biosynthesis of the essential amino acids valine, leucine and isoleucine, hence stopping cell 

division and plant growth. HED previously assessed and recommended in favor of the food uses 

of this herbicide on sugar beets and chicory (M. Doherty, et al., D260076, 12 February 2002). 

Nomenclature and the physicochemical properties of triflusulfuron-methyl are summarized in 

Tables 1 and 2, below.

TABLE 1. Test Compound Nomenclature.

Compound Chemical Structure

SO2NHCONH

N

N

N

N(CH3)2

CH3 OCH2CF3

CO2CH3

Common name Triflusulfuron-methyl

Company experimental name DPX 66037

IUPAC name methyl 2-[4-dimethylamino-6-(2,2,2-trifluoroethoxy)-1,3,5-triazin-2-

ylcarbamoylsulfamoyl]-m-toluic acid

CAS name methyl 2-[[[[[4-(dimethylamino)-6-(2,2,2-trifluoroethoxy)-1,3,5-triazin-2-yl]amino] 

carbonyl]amino]sulfonyl]-3-methylbenzoate

CAS # 126535-15-7
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End-use product/(EP) UpBeet Herbicide*

*The EP used in the submitted garden beet field trials under review was Safari 50DF, an EP that is equivalent to that of UpBeet. 

TABLE 2. Physicochemical Properties of the Triflusulfuron-Methyl.

Parameter Value Reference

Melting point/range 155-158oC PMRA REG99-03

pH Not indicated

Density (g/mL) 1.45 absolute

0.56 (Bulk)

Water solubility ( 25°C) pH ppm

3 1.0

5 2.7

7 110

9 11 000

PMRA REG99-03

Low solubility at pH3 and pH 5; soluble at 

pH 7; very soluble at pH 9. Solubility 

increases with increasing pH.

Solvent solubility (mg/L) solvent mg/L

hexane <0.0016

octanol 0.026

toluene 2.0

methanol 7.0

ethyl acetate 27

acetonitrile 80

acetone 120

chloroform 160

methylene chloride 580

Vapor pressure at 25°C <1 x 10-7 mm Hg

A relatively non-volatile compound; low 

potential for losses due to volatilization.

Dissociation constant (pKa) 4.4

This compound will dissociate and exist in its 

anionic form at most environmentally 

relevant pHs.

Octanol/water partition coefficient (KOW) pH Kow at 25°C

5 220

7 9.2

9 0.86

Low potential for bioaccumulation in biota.

UV/visible absorption spectrum λmax = 395 nm

absorption at λ>400 nm is not anticipated

Potential to absorb sunlight in the UV/visible 

range of 290-400 nm.

860.1200 Directions for Use

Summary of Existing Uses on Garden Beet and Chicory

For sugar beet, UpBeet may be broadcast applied via ground or air equipment at up to 0.032 lb 
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ai/A/application. Multiple applications can be made at 5-10 day re-treatment intervals (RTIs)

with the total application not to exceed 0.078 lb ai/A/season. For chicory, two applications may

be made, at 0.024 lb ai/A and at 5- to 10-day RTIs, for a maximum seasonal application rate of 

0.048 lb ai/A. This formulation may also be band-applied at rates equivalent to the broadcast 

rates (actual band rates are dependent upon crop row spacing). UpBeet is not to be applied via 

any type of irrigation system. The existing label for UpBeet lists a PHI of 60 days for both sugar 

beet and chicory, representing a potential mid-season use. Plant-back intervals are 0 days for 

sugar beets, 14 days for all other crops except corn, and 21 days for corn. A plant-back interval 

has not been proposed for chicory or garden beet. HED is satisfied with the 14-day interval that 

these crops receive from being in the “other crop” category as listed on the current label.

Proposed Use on Garden Beet

Table 3 provides a summary of the proposed uses for DuPontTM UpBeet® Herbicide (DF, waterdispersible granule, WDG, containing 50% triflusulfuron-methyl by weight). The maximum 

single application rate is 0.0156 lb ai/A for agricultural use on garden beets for control of 

broadleaf weeds. Triflusulfuron-methyl (as UpBeet) may be applied aerially or with 

groundboom equipment. 

TABLE 3. Summary of Proposed Directions for Use of Triflusulfuron-Methyl on Garden Beets.

Trade 

Name

Application 

Timing

Application 

Rate 

(lb ai/A)

Max. 

No. 

Applic. 

per 

Season

RTI1

(days)

Max. 

Seasonal 

Applic. 

Rate

(lb ai/A)

PHI

(days)

Use Directions and other 

Limitations

(i.e. grazing, type of 

application/equipment, 

plant-back interval, other)

Garden Beets

UpBeet® 

Herbicide

Apply as a postemergent spray 

when garden 

beets are at the 

following 

stages: 2-4 leaf 

stage; 4-6 leaf 

stage; and 6-8 

leaf stage.

0.0156 

+ 

NIS or COC

(at 0.25% v/v)

3 See 

application 

timing

0.047 30a - Apply UpBeet® Herbicide 

with NIS or COC.

- Apply UpBeet® Herbicide 

via groundboom or aerial 

application equipment.b 

- Do not apply UpBeet® 

Herbicide through any type 

of irrigation equipment.

1 RTI = retreatment interval

Notes:

a The label does not indicate a specific PHI for garden beets, however there is a general label statement that appears on the new 

proposed supplemental label indicating that “UpBeet® Herbicide must not be applied within 60 days of harvest” (PMRA# 

1708273). The letter from the sponsor (PMRA# 1708271) and the letter of support from the registrant (PMRA# 1708272) both 

indicate a PHI of 30 days. According to the D.3.1 note to file (M2004-4211/cc050119.ntf.wpd), a clarifax was sent on 13 January 

2005 to the sponsor to clarify the correct PHI. The sponsor indicated that the correct PHI is 30 days. Therefore, a specific PHI 

of 30 days for garden beets needs to be added to the supplemental label. 

b

The petition under review (9E7669, pg. 17) for the new use on garden beets lists both ground and aerial application methods. 

The existing label for sugar beets covers both of these application methods. The proposed supplemental label for garden beets 

covers only the ground application method. The sponsor has confirmed that aerial application is requested for this new use. 

Therefore, the aerial application method needs to be added to the supplemental label. 
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Conclusions. The proposed labels are adequate to evaluate the residue data relative to the 

proposed uses. However, the proposed supplemental label for garden beet needs to be revised to 

reflect the requested 30 day PHI and the aerial application method.

Therefore, the following label amendments are required for the proposed supplemental label for 

use of UpBeet® Herbicide (Reg. No. 352-569) on garden beet:

1) Under the Directions for Use section: 

ADD: Do not apply within 30 days of harvest.

2) Aerial application method – The use of the aerial application method needs to be delineated, 

either by reference to the existing label instructions for aerial application on sugar beets or by

including similar language on the supplemental label itself. 

860.1300 Nature of the Residue - Plants

MRID 42496866 – Sugar Beet

The initial step in the metabolic breakdown of triflusulfuron-methyl on sugar beet plants appears 

to be cleavage of the sulfonylurea bridge, forming triazine urea and ester sulfonamide. This 

process appears to occur readily on the leaf surfaces of the sugar beet. Triazine urea undergoes 

reduction and demethylation steps over time, eventually forming N,N-bisdesmethyl triazine 

amine. The ester sulfonamide intermediate is rapidly converted to acid sulfonamide, which, over 

time, appears to form a conjugate with glucose.

The confined rotational crop studies show that cleavage of the sulfonylurea bond also occurs 

readily in soil. No parent compound was detected in any plant commodities, even from the 

shortest rotation interval of 30 days, suggesting that it is rapidly cleaved and/or not translocated. 

However, the triazine side of the triflusulfuron-methyl molecule appears to be more systemic 

and/or more persistent than the sulfonamide portion. 

Conclusions: For the purposes of this petition (9E7669) on garden beet, the previously reviewed 

sugar beet metabolism study is sufficient to support the new requested uses of triflusulfuronmethyl on garden beets. The proposed tolerance expression should include only the parent 

compound, triflusulfuron-methyl, as determined by HED [Per memo of J. Stokes (February

1996), meeting of 16 January 1996].

860.1300 Nature of the Residue – Livestock

MRID 42991431

Based on the characterization performed on the liver, kidney, and milk (triazine-labeled only for milk), 

the principle pathways for metabolism of triflusulfuron-methyl in the goat appearto be hydrolysis of the

sulfonylurea linkage and demethylation of the dimethylamino group attached to the triazine ring (either 

before of after the sulfonylurea linkage is split). The triazine-labeled studies show that additional 

metabolic pathways exist that involve conjugation with endogenous proteins, producing a high 
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proportion of covalently bound or highly polar, soluble residue.

Conclusions: HED previously reviewed this goat metabolism study and cited deficiencies to be 

addressed if additional Section 3 registrations were pursued by the petitioner. However, HED 

has determined that measurable triflusulfuron-methyl residues above the limit of detection (LOD;

0.005 ppm) will not be detectable in milk, meat, fat, muscle, or meat byproducts from the 

existing use on sugar beets and the new proposed use on garden beet. Therefore, the deficiencies 

cited in the HED memorandum of 7 June 1993 (J. Herndon) do not need to be addressed for the 

purposes of this new food use on garden beet. However, if future uses on large-acreage crops 

such as wheat, corn, or soybean are proposed, and consequently involve feedstuffs, then an 

adequate ruminant metabolism study should be provided.

860.1340 Residue Analytical Methods

MRID 42496873 – Method AMR 1930-91

Method AMR 1930-91 is the enforcement method on record for triflusulfuron-methyl, which was 

independently validated (MRID 42517103). Frozen beet foliage or beet root samples are chopped 

prior to extraction. Samples (10 g.) are extracted with 20% acetonitrile (ACN)/80% 0.1M 

(NH4)2CO3 buffer, macerated, centrifuged, and the resulting supernatant drawn off. The 

supernatant is acidified to between pH 2-3 and recentrifuged. The solutions are passed through 

phenyl solid phase extraction (SPE) cartridges and acidified (pH <3). The samples are further 

purified using a phenyl HPLC column and UV detector (232nm). The samples are eluted using 

0.01 M potassium phosphate in 60% methanol (pH 3.5) and the fractions eluting around DPX66037 (retention time, RT, verified with a standard) are collected. The samples are acidified to 

between pH 2-3 and injected onto a C18 HPLC column, eluted with 0.01 M potassium phosphate in 

42% methanol (pH 7.0), with the DPX-66037 peak detected by UV (232nm). The LOQ is 0.05 

ppm.

MRID 42496872 – Method AMR-2021-91

This AMR 2021-91 method is very similar to the enforcement method, AMR 1930-91. By using 

automated column switching equipment for the columns and eluate, the sample transfer from the 

phenyl to C18 column can occur automatically. In addition, without the sample loss that occurs in 

the transfer of sample from the phenyl to C18 column (only 2 mL of the 10 mL is transferred to the 

C18 column in the AMR 1930-91 method), the method is able to achieve a lower LOQ (the 

registrant claims 0.02 ppm). For sugar beet roots at fortifications from 0.020 to 1.00, the registrant 

achieved recovery values from 89 to 111% (average recovery of 101%, standard deviation of 7%). 

For sugar beet foliage at fortifications from 0.020 to 1.00, the registrant achieved recovery values 

from 89 to 106% (average recovery of 98%, standard deviation of 5%).

MRID 48207101 – Method 4480 

E.I. du Pont’s Method 4480 is proposed as a data-gathering method in plant matrices for the 

determination of residues of triflusulfuron-methyl (also referred to as DPX-66037). Two 
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separate HPLC protocols were developed, differing in detection instrumentation, with similar 

extraction and clean-up procedures for both methods.

Processed samples of red beet and sugar beet matrices (roots and foliage) are extracted by

homogenization with ACN/0.1 M ammonium carbonate (2:1, v:v). Sodium chloride (NaCl) is 

added to induce phase separation and residues of triflusulfuron-methyl partition directly into the 

ACN layer. An aliquot of the ACN layer is removed, diluted with 0.1 M acetic acid and 

subjected to sequential clean-up by SPE using Bond Elut® and/or OasisTM HLB cartridges. 

Residues of triflusulfuron-methyl are eluted with ACN, and the eluate is evaporated to dryness 

and redissolved in methanol (MeOH)/water (25:75, v:v) prior to analysis by reverse-phase 

HPLC, with either tandem mass spectrometry (MS/MS) or ultraviolet (UV) detection.

The methods are sensitive to the proposed analyte, with LOQs of 0.01 ppm for each method. 

While the LOD was not determined experimentally, it is assumed to be approximately 3 times 

the background around the RT of the peak of interest. In sugar beet and red beet samples spiked 

at the LOQ and analyzed by HPLC/MS/MS following OasisTM HLB clean-up only, recoveries 

ranged from 81% to 104%, with all standard deviations (SDs) less than or equal to 8%. In

samples cleaned-up by sequential application to SAX and OasisTM HLB cartridges and analyzed 

by HPLC/MS/MS, recoveries in the same matrices ranged from 82% to 101% when spiked at the 

LOQ, with SDs less than or equal to 10%. In samples spiked at the LOQ and analyzed by

column-switching HPLC-UV, recoveries ranged from 82% to 105%, with SDs less than or equal 

to 10%. The good recoveries and low SDs in spiked samples indicate that Method 4480 is 

reliable and reproducible, regardless of the clean-up procedures and detection methods used.

Control chromatograms were provided for sugar beet foliage, sugar beet root, red beet foliage 

and red beet root. No interferences were noted at or near the RTs of triflusulfuron-methyl, 

indicating that the method is specific for the analyte in question. There was no interference from 

reagents reported. Detector linearity was demonstrated for both methods, with coefficients of 

determination (r2) greater than 0.9900 in sugar beet foliage analyzed by HPLC/MS/MS and red 

beet foliage analyzed by column switching HPLC-UV. Extraction efficiency was not 

demonstrated for Method 4480.

Conclusions: The review of Method 4480 was originally completed by PMRA, but has been 

reviewed by HED and is consistent with OCSPP policy. An adapted version of Method 4480 

was utilized in the garden beet field trials submitted for this new use petition. 

The independent laboratory validation (ILV) of Method 4480 successfully demonstrated the 

reliability of the HPLC/MS/MS method for the analysis of residues of triflusulfuron-methyl in 

sugar beet foliage and sugar beet roots on the first attempt. Method 4480 also included a 

scientifically acceptable rationale to waive the requirement of a radiovalidation study for this 

method, based on the similarities in extraction steps between this method and those employed in 

a corresponding sweet potato metabolism study (received and reviewed by PMRA). Therefore, 

method 4480 is considered acceptable as a data-gathering and enforcement method for the 

determination of triflusulfuron-methyl in plant matrices (including garden beets). 
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The enforcement method on record for triflusulfuron-methyl is method AMR 1930-91 (MRID 

42496873). Method AMR 2021-91 (MRID 42496872) is very similar to AMR 1930-91 and was 

used in the previously submitted sugar beet and chicory field trials. Method 4480 is also similar to 

both these methods, with the primary difference being the addition of a tandem mass spectrometric 

detection protocol in Method 4480. The registrant should clarify whether they want to replace the 

existing enforcement method with Method 4480 when applying for future new uses, as this newer 

method appears to be an acceptable enforcement method. 

860.1360 Multiresidue Methods 

MRID 42496874

Analysis of DPX-66037 was not applicable to FDA Multi-Residue Protocol A (due to lack of Nmethylcarbamate structure), Protocol B (due to lack of an acid of phenol structure), or Protocol E 

(due to lack of adequate recovery from Florisil). Testing through Protocol C produced responses 

that were greater than 50% full scale deflection using several column/detector combinations. The 

laboratory noted that these responses may have been due to thermal degradation of DPX-66037 

rather than elution of DPX-66037 itself. Recoveries of DPX-66037 from sugar beets were tested 

using Protocol D. A 2% diethylene glycol succinate (DEGS) on Chromosorb W-AW packed 

column was used in conjunction with an electrolytic conductivity detector (ELCD) operated in the 

nitrogen mode. The following recoveries were achieved: 67 and 99% recovery from 0.02 ppm 

fortifications, and 92 and 106% recovery from 0.1 ppm fortifications.

Conclusions: FDA’s multiresidue method Protocol D is suitable for analysis or enforcement of 

triflusulfuron-methyl residues. This information was previously forwarded to FDA.

860.1380 Storage Stability

Previously submitted storage stability data indicate that triflusulfuron-methyl in/on sugar beet 

roots and tops is stable for periods of up to 18 months when stored frozen at -25C. The storage 

stability data submitted in support of the proposed use in garden beet roots and tops (leaves) 

demonstrated that residues were stable for up to ~15 months (436 days) when stored frozen at 

-29C to -10C. 

Conclusions: The available storage stability data indicate that residues of triflusulfuron-methyl 

are stable in frozen storage for up to approximately 15 months. Samples from the garden beet 

residue field trials were stored for up to ~13 months (381 days). Therefore no additional storage 

stability data are needed for the proposed use on garden beet.

860.1400 Water, Fish, and Irrigated Crops

There are no proposed uses for triflusulfuron-methyl that are germane to this guideline. No 

evaluation is necessary at this time.

860.1460 Food Handling
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There are no proposed uses for triflusulfuron-methyl that are germane to this guideline. No 

evaluation is necessary at this time.

860.1480 Meat, Milk, Poultry, and Eggs

In an earlier review (PP#4F4278), the petitioner was granted a waiver of ruminant and poultry

feeding studies (and the tolerances in meat, milk, poultry, and egg associated with them) for the 

existing use on sugar beets. 

The ruminant feeding study waiver was granted on the basis of estimated dietary consumption by

dairy cattle of triflusulfuron-methyl from the three feed items associated with sugar beets –

leaves, molasses, and dried pulp. In the goat metabolism study the highest residue values (total 

radioactivity, expressed as triflusulfuron-methyl equivalents) occurred in the kidneys at 0.66 ppm 

and liver at 0.61 ppm (based on feeding 10 ppm of triazine-labeled triflusulfuron-methyl in the 

diet). The maximum expected residue level in ruminant liver/kidney was found to be <0.003 

ppm, well below the LOQ of the enforcement methodology for triflusulfuron-methyl residues in 

sugar beet commodities and the likely LOQ of a reasonable livestock analytical method. 

There are no ruminant feed items associated with the new proposed use on garden beets. 

Therefore this new use should have no additional impact on the estimated ruminant dietary

consumption of triflusulfuron-methyl. 

Sugar and garden beet products are not utilized as poultry feedstuffs, therefore triflusulfuronmethyl residues would not be expected in poultry commodities. 

Conclusions: Using information in the goat metabolism studies, residues of triflusulfuronmethyl (as defined by the livestock residue definition) are expected to be below the LOQ of a 

reasonable livestock analytical method. Hence a 40 CFR 180.6(a)(3) situation (no expectation of 

finite residues) exists. Neither feeding studies nor tolerances in livestock commodities are 

required at this time. Feeding studies and analytical methods may be required to support future 

uses of triflusulfuron-methyl that involve significant livestock feed items. 

860.1500 Crop Field Trials

Sugar Beet 

MRID 42496868, 42496867

No additional field trial residue data for sugar beets were submitted in this petition. The 

registrant previously provided 3 volumes of residue data on sugar beet in PP 3G4161 (MRID#’s 

above, see 6/7/93 G. J. Herndon review, D184250).

The registrant conducted residue trials in 1991 at sites in North Dakota, Michigan, Colorado, 

Nebraska, and California. These 5 states account for 47% of the sugar beet for sugar production

(Agricultural Statistics, 1994, USDA). Three applications of a 50% DF formulation were made 

one week apart to each treated plot at 0.5 or 1.0 oz ai/A (0.03 or 0.06 lb ai/A), which corresponds 
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to between 2X and 4X the maximum proposed seasonal use rate. Samples were harvested 

between 101 and 126 days after the last application. The samples were stored up to 8 months 

between harvest and analysis. The samples were analyzed by method AMR 2021-91 (MRID 

42496872) for the parent compound (average recovery 92%), and method AMR 2187-91 (MRID 

42496875) for the 3 triazine metabolites (average recoveries of 79 to 81%). No residues of 

triflusulfuron-methyl, or its metabolites D8526, E7710, or M7222 were found in any of the 

samples (0.05 ppm LOQ for each analyte; LOD estimated at 0.005 ppm for parent and 0.02 ppm 

for triazine metabolites).

The registrant included additional, non-GLP data from trials conducted in France. At similar 

application rates and PHIs from 119 to 162 days, no triflusulfuron-methyl was found (0.02 ppm 

LOD). No residues of D8526 or E7710 were found in any samples. Quantities of M7222 were 

detected in sugar beet foliage at levels up to 0.05 ppm at 160 days. This is based on an LOD of 

0.02 ppm. 

The registrant conducted field trials to determine the decline of triflusulfuron-methyl residues 

and the formation and decline of residues of the triazine metabolites D8526, E7710, and M7222 

in sugar beet over time (MRID 42496867). Field trials were conducted in Idaho, North Dakota, 

and California in 1990 and 1991. Three applications of either 40% or 50% DF formulations 

were made one week apart at either 0.4 or 0.8 oz ai/A (0.025 or 0.05 lb ai/A) or 0.5 or 1.0 oz ai/A 

(0.03 or 0.06 lb ai/A), which corresponds to between 1.6X and 4X the maximum proposed 

seasonal use rate. Samples were harvested at intervals of about 0, 7, 14, 28, 56, 84, and 112 days 

following the last application. The whole plant was analyzed up to 28 days after last application; 

at 56 days and beyond, tops and roots were analyzed separately. The samples were stored up to 

18 months between harvest and analysis. The samples were analyzed by method AMR 2021-91 

for the parent compound (average recovery 93%), and method AMR 2187-91 for the 3 triazine 

metabolites (average recoveries of 82 to 86%). Up to 2.56 ppm of triflusulfuron-methyl was 

found on the whole beet plants at 0 days after treatment (DAT). At the next harvest, 7 DAT, and 

beyond, no parent was detected in any of the samples (the next sampling of the Idaho trial 

occurred at 28 DAT, not 7 DAT, due to severe hail damage). This is based on an LOD of 0.005 

ppm and an LOQ of 0.05 ppm. At 7 DAT and beyond (28 DAT for Idaho trial), no residues of 

D8526 or E7710 were found in any samples. Trace quantities of M7222 (0.03 ppm) were 

detected in sugar beet foliage as long as 112 DAT in two of the three trials. This is based on an 

LOD of 0.02 ppm and an LOQ of 0.05 ppm.

MRID 43982901

In response to the Agency's request for field trial data reflecting the 60 day PHI, additional data 

(MRID 43982901, see 5/14/96 J. Stokes review, D225643) from 12 trials conducted in the US 

(5), Germany (6), and Sweden (1), as well as comments on the US sugar beet production areas 

and possible PHIs (based upon US agricultural growing practices for sugar beet) were submitted. 

The PHIs of the US trials ranged from 45 to 56 days at rates of 1.4 to 6 oz ai/A (0.09 to 0.375 lb 

ai/A; 1.9X to 8X the proposed label seasonal maximum). In the Germany trials (conducted at 

1.1X the maximum proposed seasonal label rate) samples were harvested and analyzed from 15, 

16, 16, 26, 34, and 53 days after the last application. In the Sweden trial, samples were analyzed 
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33 days after the last application. Triflusulfuron-methyl residues were not detected (<0.02 ppm) 

in the samples. 

Therefore, the field trial and residue decline data demonstrate that triflusulfuron-methyl is rapidly

degraded after application to sugar beet plants. 

Chicory 

MRID 45211101

No additional field trial data was submitted with this petition. The results from the supervised 

crop field trials study (MRID 45211101; see 11/9/01 G. J. Herndon review, D279136) in chicory

conducted in Mt. Vernon, WA, La Conner, WA, Sedro Woolley, WA, and Manteca, CA, showed 

that the maximum residues in chicory, collected 59 to 62 days following the last application of 

triflusulfuron-methyl (as 50% ai WDG) and treated at 0.75 oz ai/A (0.05 lb ai/A, equivalent to 

1X the approved use rate), were below the LOD (0.016 ppm). The residues in these field trials 

were determined using DuPont Method AMR 2021-91. These data were sufficient to establish a 

0.05 ppm tolerance in chicory roots. Data were also provided for dry pulp and inulin, although 

tolerances were not needed for these commodities. These analytical results adequately

demonstrated that in this limited setting the proposed use pattern would not result in residues that 

exceed this tolerance. 

Garden Beet

MRID 47943201

The sponsor, Interregional Research Project No. 4 (IR4), has submitted field trial data for 

triflusulfuron-methyl on garden beets. Five garden beet field trials were conducted in the United 

States and 3 field trials were conducted in Canada for a total of eight field trials conducted during 

the 2005 growing season. In the United States, trials were located in New York (Zone 1), Ohio 

(Zone 5), Oregon (Zone 12), Texas (Zone 6) and Wisconsin (Zone 5A). In Canada, two trials 

were conducted in Quebec (Zone 5B) and one trial was conducted in Ontario (Zone 5). At each 

trial site, garden beets were treated with triflusulfuron-methyl (formulated as Safari 50 DF) in 3 

foliar broadcast applications at a rate of 0.016 – 0.017 lb ai/A/application, at 5-12 day RTIs, for a 

maximum seasonal application rate ranging from 0.047 – 0.049 lb ai/A. Garden beet roots and 

tops (leaves) were harvested at PHIs of 22-56 days. A registered NIS or COC was added to the 

spray mixture for each application at a rate of 0.25% v/v at all trials except the trial conducted in 

Oregon.

Garden beet samples were analyzed for residues of triflusulfuron-methyl using a modified 

version of DuPont method 4480 entitled “Analytical Method for the Determination of 

Triflusulfuron-Methyl in sugar beets and red beets by HPLC/MS/MS and column switching 

HPLC-UV” (PMRA# 1708274). This method has been previously reviewed and deemed 

acceptable for both data-gathering and enforcement (M2005-0813 – PMRA# 1041650). 

The method was adequately validated in garden beet roots and tops at spiking levels of 0.01 ppm, 

0.10 ppm, and 1.00 ppm (recoveries ranged from 94-118% in roots and from 90-107% in tops) 

indicating that the modifications to the method did not adversely affect the method’s data-
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gathering capabilities. The instrument’s LOQ for this method was set at the lowest level of 

method validation (LLMV) of 0.01 ppm for each matrix (roots and tops). The 

calculated/statistical LOQ values were 0.0069 ppm for roots and 0.0044 ppm for tops. The 

calculated/statistical LOD values were 0.0023 ppm for roots and 0.0015 ppm for tops. Method 

suitability was also evaluated concurrently with sample analysis. Mean concurrent recoveries ± 

SD in garden been roots and tops spiked with 0.01 ppm (1x LOQ) of triflusulfuron-methyl were 

99 ± 9% (n = 8) and 98 ± 5% (n = 8), respectively. All recoveries fell within the generally

recognized acceptable 70-120% range indicating that DuPont method 4480 is reliable for the 

determination of residues of triflusulfuron-methyl in/on garden beet roots and tops.

Treated garden beet root and top samples were stored frozen (-29oC to -10oC) for 299-377 days 

and 300-381 days, respectively. The freezer storage stability of triflusulfuron-methyl residues 

in/on garden beets was demonstrated concurrently, with samples spiked at 0.10 ppm. The mean 

± SD corrected residue recoveries (corrected for concurrent recoveries of 114% and 107% in 

roots and tops, each spiked with 0.10 ppm of triflusulfuron-methyl, respectively) of 

triflusulfuron-methyl in samples stored frozen for 436 days were 92 ± 3% (n = 3) in roots and 92 

± 2% (n = 3) in tops, indicating that no significant degradation of residues occurred throughout 

this time interval. Therefore, there are no concerns with the stability of residues over time in this 

study.

The data indicate that residues of triflusulfuron-methyl in/on garden beet roots were all below the 

LOQ of 0.01 ppm when treated at total rates of 0.047-0.048 lb ai/A (using a NIS/COC) and 

harvested at PHIs of 22-56 days. Residues of triflusulfuron-methyl in/on garden beet tops 

(leaves) were ≤ 0.01 ppm when treated at total rates of 0.047-0.048 lb ai/A (using a NIS/COC) 

and harvested at PHIs of 22-56 days. At the Oregon trial site where no NIS/COC was used in the 

spray mixtures, residues in roots and tops treated with a total of 0.049 lb ai/A and harvested at a 

PHI of 24 days were all <0.01 ppm. Residue decline behaviour was not assessed in this study. 

TABLE 4. Summary of Residue Data from Crop Field Trials with Triflusulfuron-Methyl.

Commodity Total Applic. 

Rate

 (g a.i./ha)

PHI 

(days)

Triflusulfuron-methyl Residue Levels

(ppm)

n Min. Max. HAFT* Median

(STMdR)

Mean

(STMR)

Std. Dev.

WITH a Non-Ionic Surfactant or Crop Oil Concentrate

Garden Beet 

Tops (Leaves)

53.0 – 54.3a22-56 14 <0.01 0.01 <0.01 <0.01 <0.01 0.00

Garden Beet 

Roots

53.0 – 54.3 22-56 14 <0.01 <0.01 <0.01 <0.01 <0.01 0.00

WITHOUT a Non-Ionic Surfactant or Crop Oil Concentrate

Garden Beet 

Tops (Leaves)

54.6b24 2 <0.01 <0.01 <0.01 <0.01 <0.01 0.00

Garden Beet 

Roots

54.6 24 2 <0.01 <0.01 <0.01 <0.01 <0.01 0.00

a

53.0-54.3 g ai/ha = 0.047-0.048 lb ai/A

b

54.6 g ai/ha = 0.049 lb ai/A

* HAFT = Highest Average Field Trial.

STMdR = supervised trial median residues
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STMR = supervised trial mean residues

Std. Dev. = standard deviation

Conclusions: The crop field trials for chicory and sugar beets were previously classified 

acceptable and satisfied the guideline requirement for crop field trials (M. Doherty, et al., 

D260076, 12 February 2002). 

The newly submitted crop field trials are adequate to support the requested new use of 

triflusulfuron-methyl on garden beet. The review of these field trials was originally conducted by

PMRA under a work share agreement, but their conclusions have been reviewed by HED and are 

consistent with OCSPP policy. The freezer storage stability of triflusulfuron-methyl residues 

in/on garden beets was demonstrated concurrently, with samples spiked at 0.10 ppm stored 

frozen for 436 days. Analysis of these samples showed no significant degradation of residues 

occurred throughout this time interval. The maximum frozen storage time for garden beet 

samples was 381 days, a duration that is shorter than the demonstrated residue stability period. 

Therefore, there are no concerns with the stability of residues over time in this study.

The data indicates that residues of triflusulfuron-methyl in/on garden beet roots and tops were all 

≤ 0.01 ppm when treated at total rates of 0.047-0.049 lb ai/A (using a NIS/COC or not) and 

harvested at PHIs of 22-56 days. It should be noted that the petition under review requests a 

30-day PHI for garden beet, whereas a 60-day PHI is currently approved for sugar beets. The 

requested 30-day PHI for garden beet is supported by the submitted field trial data. Of the 8 

submitted garden beet field trials, 5 were conducted at PHIs of 30 days or less (22, 23, 24, 24, 30) 

and 3 at PHIs greater than 30 days (36, 42, 56). 

860.1520 Processed Food and Feed

Processing data were not submitted for garden beets. Such data are not required as there are no 

processed commodities from garden beets in which residues are expected to concentrate.

A previously submitted processing study (MRID 42496869; see 6/7/93 G. J. Herndon review, 

D184250) was performed in North Dakota where plots of sugar beet were treated one time at 45 

days before harvest with a 50% DF formulation of triflusulfuron-methyl at the rate of 6.0 oz ai/A 

(0.38 lb ai/A; 8X the proposed seasonal use rate). At harvest, roots and tops were separated and 

frozen. Sugar beet roots were processed into white sugar, molasses, and dried pulp, and the 

samples were shipped frozen to the registrant for analysis. Samples were analyzed using method 

AMR-2021-91 with modifications made to the sample extraction step for the processed fractions. 

Recoveries were as follows: sugar (range: 85 to 104%, average: 97%), molasses (range: 83 to 

97%, average: 92%), and dried pulp (range: 86 to 111%, average: 97%).

Up to 0.033 ppm of triflusulfuron-methyl was found in the sugar beet tops at harvest (45 DAT). 

No triflusulfuron-methyl was found in the samples of sugar beet root, the raw agricultural 

commodity (RAC), or in any of the processed commodities (sugar, molasses, or dried pulp). 

This is based on a 0.01 ppm LOD. No analyses were performed to examine if any of the three 

triazine metabolites, D8526, E7710, and M7222, were present in any of the samples.
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Conclusions. Based on the available data, the field trial and residue decline data demonstrate 

that triflusulfuron-methyl is rapidly degraded after application to sugar beet plants. Even at 

exaggerated rates (8X), no triflusulfuron-methyl was found in the sugar beet roots (RAC) or 

processed commodities; therefore concentration of residues in processed commodities is unlikely

at the currently approved use rates on sugar beets. Additional residue decline data was not 

submitted for the garden beet proposed use currently under review. There are no known 

processed commodities for garden beets in which residues are expected to concentrate; thus,

processed food and feed should not be an issue for this new food use petition. 

860.1650 Submittal of Analytical Reference Standards

An analytical standard for triflusulfuron-methyl is currently available in the NPSR, via personal 

communication with Dallas Wright, of ACB, on October 4 2010. Analytical reference standards 

should be replenished as requested by the Repository. The reference standards should be sent to 

the ACB, which is located at Fort Meade, to the attention of Theresa Cole at the following 

address: 

USEPA

National Pesticide Standards Repository/Analytical Chemistry Branch/OPP

701 Mapes Road

Fort George G. Meade, MD 20755-5350

(Please note that the mail will be returned if the extended zip code is not used.)

860.1850 Confined Accumulation in Rotational Crops

A confined rotational crop study was conducted using wheat, beetroot (red beet), and lettuce sown 

30, 120, and 300 days post-application and grown to maturity (MRID 42496865). The study was 

conducted using triflusulfuron-methyl labelled separately with carbon-14 in the triazine ring and at 

the ester carbonyl position, similar to the plant and animal metabolism studies.

The confined rotational crop studies show that cleavage of the sulfonylurea bond also occurs readily

in soil. No parent compound was detected in any plant commodities, even from the shortest 

rotation interval of 30 days, suggesting that it is rapidly cleaved and/or not translocated. However, 

the triazine side of the triflusulfuron-methyl molecule appears to be more systemic and/or more 

persistent than the sulfonamide portion. Uptake of triazine residues into wheat straw has been 

observed.

Conclusions: Additional rotational crop studies or rotational crop tolerances are not needed at this 

time. Such studies may be needed if uses are proposed on major crops (e.g., wheat, cotton, corn, 

soybeans) due to the observed uptake of triazine-derived soil residues into wheat straw in the 

confined study. [See memo of J. Stokes (31 January 1996) for HED meeting of 16 January 1996].
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860.1900 Field Accumulation in Rotational Crops

Additional rotational crop studies or rotational crop tolerances are not needed at this time. Such 

studies may be needed if uses are proposed on major crops (e.g., wheat, cotton, corn, soybeans).

860.1550 Proposed Tolerances

At this time, tolerances are not required in livestock commodities. For plant commodities, 

existing tolerances are in place for the previously assessed sugar beets and chicory. New 

tolerances in/on garden beets are being requested in the petition under review (Petition 9E7669). 

The registrant has proposed that tolerances for the residues of the herbicide triflusulfuronmethyl, or methyl 2-[[[[[4-(dimethylamino)-6-(2,2,2-trifluoroethoxy)-1,3,5-triazin-2-

yl]amino]carbonyl]amino]sulfonyl]-3-methylbenzoate, be established on garden beet roots at 

0.01 ppm (parent only) and sugar beet tops at 0.02 ppm (parent only). For the purposes of this 

tolerance petition, the compound to be regulated is parent only. 

A summary of the petitioned-for tolerances and HED’s recommendations is provided in Table 5, 

below. The requested new use on garden beet is being assessed as part of a work share 

agreement with Canada’s PMRA. Therefore, implemented tolerances stemming from this new 

use will be harmonized with the PMRA review. The recommended tolerance for garden beet 

roots of 0.01 ppm is identical to that of PMRA’s, with all garden beet roots having 

concentrations less than the LOQ of 0.01 ppm in observed field trials. However, the 

recommended tolerance of 0.02 ppm for garden beet tops is not harmonized with PMRA’s value 

of 0.01 ppm. Based on the submitted garden beet field trial data, garden beet tops had 

concentrations equal to 0.01 ppm at a single field trial in Texas (Trial 08043.05-TX*19). Given 

the level of uncertainty for quantification around the LOQ of 0.01 ppm and the limited number of 

field trials, HED does not consider setting the garden beet top tolerance at 0.01 ppm to be 

appropriate. Therefore, HED is concurring with the registrant-proposed tolerance of 0.02 ppm 

for garden beet tops. 

Table 5. Tolerance Summary for Triflusulfuron-methyl

Commodity Proposed 

Tolerance (ppm)

Recommended 

Tolerance (ppm)

Comments (correct commodity definition)

Beet, garden, roots 0.01 0.01 --

Beet, garden, tops 0.02 0.02 --

References

Triflusulfuron-methyl: Human Health Risk Assessment for the Section 3 Registration on Sugar

Beets (PP#4F4278) and Chicory (PP#0E6214). (M. Doherty, Ph.D., D260076; February 2002) 

PP#4F4278. Triflusulfuron-methyl (DPX-66037) on Sugarbeets. Evaluation of Analytical Method and

Residue Data. MRID#s 429914-01, 429914-02, and 429914-31. DP Barcodes# 211798 and 211802.

CBTS# 15084 and 15085. (J. Stokes, January 31, 1996) 
Triflusulfuron-methyl (129002) Summary of Analytical Chemistry and Residue Data Barcode: D388620

Page 19 of 21

PP#4F4278. Triflusulfuron-methyl (DPX-66037) In/On Sugarbeet. Additional Comments on 

Proposed 60-Day PHI. Product Chemistry. MRID#s 439829-00 and 439829-01. DP Barcode# 

D225643. (J. Stokes, May 14, 1996)

PP#3G04161. Temporary Tolerance Petition and Experimental Use Permit for Use of 

Triflusulfuron-methyl (DPX-66037) on Sugar Beets; 000352-EUP-RAN. Evaluation of Analytical 

Method and Residue Data. (G. J. Herndon, D184250, 6/7/93)

MRID Study Title Date

42496865

Hawkins, D.; Kirkpatrick, D.; Dean, G.; et al. (1992) Confined 

Accumulation Study of (Carbon 14)-DPX-66037 on Rotational Crops: Lab 

Project Number: HRC/DPT 222/911123: AMR-1637-90. Unpublished study

prepared by Huntingdon Research Centre Ltd. 122 p. 

30-

Sep1992

42496866

Dietrich, R.; McAleer, N. (1992) Metabolism of DPX-66037 in Sugar Beets: 

Lab Project Number: EF-90-312: AMR 1639-90. Unpublished study

prepared by Pan-Agricultural Laboratories Inc.; E.I. du Pont de Nemours & 

Co. 94 p. 

30-

Sep1992

42496868

Slates, R.; Desmond, P.; Devine, P.; et al. (1992) Magnitude of Residues of 

DPX-66037 Experimental Herbicide in Sugar Beets Following Multiple 

Applications: Lab Project Number: AMR 2004-91. Unpublished study

prepared by E. I. du Pont de Nemours & Co. 258 p. 

30-

Sep1992

42496873

Amoo, J.; Walker, D. (1991) Split Analytical Method for the Quantitation of 

DPX-66037 in Sugar Beets: Lab Project Number: AMR 1930-91. 

Unpublished study prepared by E. I. du Pont de Nemours & Co. 41 p. 

30-

Sep1992

42496872

Major L.; Larochelle, J.; Bedwell, L.; et al. (1991) Automated Analytical 

Method for the Quantitation of DPX-66037 in Sugar Beets: Lab Project 

Number: AMR 2021-91. Unpublished study prepared by E. I. du Pont de 

Nemours & Co. 42 p. 

30-

Sep1992

42496874

Fomenko, J. (1991) Testing of DPX-66037 through FDA Multi-Residue 

Protocols A through E: Lab Project Number: AMR-2085-91: DUP91001. 

Unpublished study prepared by Spectralytix Inc. 69 p. 

30-

Sep1992

42517103

Bruns, G. (1992) Independent Laboratory Validation of DPX-66037 in Sugar 

Beet Foliage and Roots by Liquid Chromatography: Lab Project Number: 

AMR 2194-91: 92-P1143.REP. Unpublished study prepared by Enviro-Test 

Laboratories. 142 p. 

30-

Sep1992

45211101

Salzman, F. (2000) Triflusulfuron-Methyl: Magnitude of the Residue on 

Chicory (Roots): Lab Project Number: 06709.99-ABC03: 06709.97-WA63: 

06709.97-WA64. Unpublished study prepared by ABC Laboratories, Inc., 

Washington State University & Plant Sciences, Inc. 223 p. {OPPTS 

12-

Sep2000
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Page 20 of 21

860.1550} 

47943201

Carpenter, D. (2008) Triflusulfuron-Methyl: Magnitude of the Residue on 

Beet (Garden). Project Number: 08043, 08043/05/MIR04, 08043/05/NY07. 

Unpublished study prepared by Interregional Research Project No. 4, IR-4 

North Central Analytical Lab and Cornell University. 247 p.

23-

Dec2009

48207101

Peterson, B.; Walker, D.; Cabusas, M. (2001) Analytical Method for the 

Determination of Triflusulfuron-Methyl in Sugar Beets and Red Beets by

HPLC/MS/MS and Column-Switching HPLC-UV. Project Number: 

AAFC03/003, XENO3/88, AAFCO3/003/OCR. Unpublished study prepared 

by E.I. du Pont de Nemours and Company. 35 p.

42991431

Hawkins, D.; Kirkpatrick, D.; Dean, G.; et al. (1993) The Metabolism of 

(carbon-14)-DPX-66037 in the Goat Following Administration at a Level 

Equivalent to 10 PPM in the Diet: Lab Project Number: AMR 1640-90: 

HRC/DPT 244/911287. Unpublished study prepared by Dept. of Chemical 

Metabolism & Radiosynthesis, Huntingdon Research Centre Ltd. 123 p. 

20-

Oct1993
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Attachment 1 – International Residue Limits 

Triflusulfuron-methyl (PC Code 129002; 10/01/10)

Summary of US and International Tolerances and Maximum Residue Limits 

Residue Definition:

US Canada Mexico2 Codex3

40 CFR 180.492:

Plants: triflusulfuron methyl 2-[[[[[4-

(dimethylamino)-6-(2,2,2-

trifluoroethoxy)-1,3,5-triazin-2-

yl]amino]carbonyl]amino]sulfonyl]-3-

methylbenzoate

methyl 2-[4-

dimethylamino-6-(2,2,2-

trifluoroethoxy)-1,3,5-

triazin-2-

ylcarbamoylsulfamoyl]- mtoluate

None

Commodity1 Tolerance (ppm) /Maximum Residue Limit (mg/kg)

US Canada Mexico2 Codex

Beet, garden, roots 0.01 None

Beet, garden, tops 0.02

Completed: M. Negussie; 10/05/10

1

Includes only commodities of interest for this action. Tolerance values should be the HED recommendations and 

not those proposed by the applicant.

2 Mexico adopts US tolerances and/or Codex MRLs for its export purposes.

3 * = absent at the limit of quantitation; Po = postharvest treatment, such as treatment of stored grains. PoP = 

processed postharvest treated commodity, such as processing of treated stored wheat. (fat) = to be measured on the 

fat portion of the sample. MRLs indicated as proposed have not been finalized by the CCPR and the CAC.