13339 Hagers Ferry Road

Huntersville, NC 28078-7929

McGuire Nuclear Complex - MG03A2

Phone: 980-875-5245 Fax: 980-875-4349

Order Summary Report

Analytical Laboratory

Order Number: J13010342

Lab Contact: Jason C Perkins

Date: 2/11/2013

Customer Address: 253 Plant Allen Road

Customer Name(s): Bill K., Ron L., Don S.

Belmont, NC 28012

Phone: 980-875-5348

Report Authorized By:

(Signature)

Program Comments:

Please contact the Program Manager (Jason C Perkins) with any questions regarding this report.

Data Flags & Calculations:

Any analytical tests or individual analytes within a test flagged with a Qualifier indicate a deviation from the method quality 

system or quality control requirement. The qualifier description is found at the end of the Certificate of Analysis (sample results) 

under the qualifiers heading. All results are reported on a dry weight basis unless otherwise noted. Subcontracted data 

included on the Duke Certificate of Analysis is to be used as information only. Certified vendor results can be found in the 

subcontracted lab final report. Duke Energy Analytical Laboratory subcontracts analyses to other vendor laboratories that have 

been qualified by Duke Energy to perform these analyses except where noted.

Data Package:

This data package includes analytical results that are applicable only to the samples described in this narrative. An estimation of 

the uncertainty of measurement for the results in the report is available upon request. This report shall not be reproduced, except 

in full, without the written consent of the Analytical Laboratory. Please contact the Analytical laboratory with any questions. The 

order of individual sections within this report is as follows:

Job Summary Report, Sample Identification, Technical Validation of Data Package, Analytical Laboratory Certificate of Analysis, 

Analytical Laboratory QC Reports, Sub-contracted Laboratory Results, Customer Specific Data Sheets, Reports & 

Documentation, Customer Database Entries, Test Case Narratives, Chain of Custody (COC)

Certification:

The Analytical Laboratory holds the following State Certifications : North Carolina (DENR) Certificate #248, South Carolina 

(DHEC) Laboratory ID # 99005. Contact the Analytical Laboratory for definitive information about the certification status of 

specific methods. 

Jason C Perkins

Page 1 of 39
Sample ID's & Descriptions:

Sample ID Plant/Station

Collection 

Date and Time Collected By Sample Description

2013001388 ALLEN 18-Jan-13 10:51 AM JBW FGD Purge Eff 

2013001389 ALLEN 18-Jan-13 8:27 AM JBW EQ Tank 

2013001390 ALLEN 18-Jan-13 8:30 AM JBW BioReactor 1 Inf 

2013001397 ALLEN 18-Jan-13 8:57 AM JBW BioReactor 1 Inf BLANK 

2013001400 ALLEN 18-Jan-13 8:40 AM JBW BioReactor 2 Inf 

2013001401 ALLEN 18-Jan-13 9:09 AM JBW BioReactor 2 Inf BLANK 

2013001402 ALLEN 18-Jan-13 8:34 AM JBW BioReactor 2 Eff 

2013001403 ALLEN 18-Jan-13 9:05 AM JBW BioReactor 2 Eff BLANK 

2013001404 ALLEN 18-Jan-13 12:10 PM JBW Filter Blk 

9 Total Samples

Page 2 of 39
COC and .pdf report are in agreement with sample totals 

and analyses (compliance programs and procedures).

All Results are less than the laboratory reporting limits.

All laboratory QA/QC requirements are acceptable.

Yes No

Technical Validation Review

Checklist:

Yes No

Yes No

Report Sections Included:

Job Summary Report Sub-contracted Laboratory Results

Sample Identification Customer Specific Data Sheets, Reports, & Documentation

Technical Validation of Data Package Customer Database Entries

Analytical Laboratory Certificate of Analysis

Analytical Laboratory QC Report

Chain of Custody

Reviewed By: DBA Account Date: 2/11/2013

Electronic Data Deliverable (EDD) Sent Separately

Page 3 of 39
Certificate of Laboratory Analysis

This report shall not be reproduced, except in full.

Order # J13010342

2013001388

Collection Date: 18-Jan-13 10:51 AM

Site: FGD Purge Eff 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

ALKALINITY - (Analysis Performed by Prism Labs)

Vendor Parameter Complete Vendor Method V_PRISM

INORGANIC IONS BY IC

Bromide 760 mg/L 100 1000 EPA 300.0 01/28/2013 14:34 JAHERMA

Chloride 2000 mg/L 100 1000 EPA 300.0 01/28/2013 14:34 JAHERMA

Sulfate 1300 mg/L 100 1000 EPA 300.0 01/28/2013 14:34 JAHERMA

MERCURY (COLD VAPOR) IN WATER

Mercury (Hg) 26.3 ug/L 2.5 50 EPA 245.1 01/24/2013 12:23 AGIBBS

DISSOLVED METALS BY ICP

Manganese (Mn) 4.10 mg/L 0.05 10 EPA 200.7 01/28/2013 12:03 MHH7131

TOTAL RECOVERABLE METALS BY ICP

Boron (B) 46.2 mg/L 0.5 10 EPA 200.7 01/29/2013 13:30 MHH7131

Calcium (Ca) 2150 mg/L 0.1 10 EPA 200.7 01/29/2013 13:30 MHH7131

Iron (Fe) 84.3 mg/L 0.1 10 EPA 200.7 01/29/2013 13:30 MHH7131

Magnesium (Mg) 374 mg/L 0.05 10 EPA 200.7 01/29/2013 13:30 MHH7131

Manganese (Mn) 4.63 mg/L 0.05 10 EPA 200.7 01/29/2013 13:30 MHH7131

DISSOLVED METALS BY ICP-MS

Selenium (Se) 833 ug/L 10 10 EPA 200.8 01/24/2013 11:27 KRICHAR

TOTAL RECOVERABLE METALS BY ICP-MS

Arsenic (As) 287 ug/L 10 10 EPA 200.8 01/24/2013 10:07 KRICHAR

Cadmium (Cd) < 10 ug/L 10 10 EPA 200.8 01/24/2013 10:07 KRICHAR

Chromium (Cr) 176 ug/L 10 10 EPA 200.8 01/24/2013 10:07 KRICHAR

Copper (Cu) 260 ug/L 10 10 EPA 200.8 01/24/2013 10:07 KRICHAR

Nickel (Ni) 268 ug/L 10 10 EPA 200.8 01/24/2013 10:07 KRICHAR

Selenium (Se) 4490 ug/L 10 10 EPA 200.8 01/24/2013 10:07 KRICHAR

Silver (Ag) < 10 ug/L 10 10 EPA 200.8 01/24/2013 10:07 KRICHAR

Zinc (Zn) 457 ug/L 10 10 EPA 200.8 01/24/2013 10:07 KRICHAR

SELENIUM SPECIATION - (Analysis Performed by Applied Speciation and Consulting, LLC)

Vendor Parameter Complete Vendor Method V_AS&C

TOTAL DISSOLVED SOLIDS

TDS 8300 mg/L 200 1 SM2540C 01/22/2013 16:15 SWILLI3

TOTAL SUSPENDED SOLIDS

TSS 3300 mg/L 250 1 SM2540D 01/24/2013 13:25 SWILLI3

Page 4 of 39
Certificate of Laboratory Analysis

This report shall not be reproduced, except in full.

Order # J13010342

2013001389

Collection Date: 18-Jan-13 8:27 AM

Site: EQ Tank 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

MERCURY (COLD VAPOR) IN WATER

Mercury (Hg) 32.6 ug/L 2.5 50 EPA 245.1 01/24/2013 12:26 AGIBBS

DISSOLVED METALS BY ICP

Manganese (Mn) 2.20 mg/L 0.05 10 EPA 200.7 01/28/2013 12:07 MHH7131

TOTAL RECOVERABLE METALS BY ICP

Boron (B) 34.9 mg/L 0.5 10 EPA 200.7 01/29/2013 13:34 MHH7131

Calcium (Ca) 1780 mg/L 0.1 10 EPA 200.7 01/29/2013 13:34 MHH7131

Iron (Fe) 115 mg/L 0.1 10 EPA 200.7 01/29/2013 13:34 MHH7131

Magnesium (Mg) 302 mg/L 0.05 10 EPA 200.7 01/29/2013 13:34 MHH7131

Manganese (Mn) 5.98 mg/L 0.05 10 EPA 200.7 01/29/2013 13:34 MHH7131

DISSOLVED METALS BY ICP-MS

Selenium (Se) 212 ug/L 10 10 EPA 200.8 01/24/2013 11:30 KRICHAR

TOTAL RECOVERABLE METALS BY ICP-MS

Arsenic (As) 282 ug/L 10 10 EPA 200.8 01/24/2013 10:10 KRICHAR

Cadmium (Cd) < 10 ug/L 10 10 EPA 200.8 01/24/2013 10:10 KRICHAR

Chromium (Cr) 208 ug/L 10 10 EPA 200.8 01/24/2013 10:10 KRICHAR

Copper (Cu) 284 ug/L 10 10 EPA 200.8 01/24/2013 10:10 KRICHAR

Nickel (Ni) 291 ug/L 10 10 EPA 200.8 01/24/2013 10:10 KRICHAR

Selenium (Se) 4720 ug/L 10 10 EPA 200.8 01/24/2013 10:10 KRICHAR

Silver (Ag) < 10 ug/L 10 10 EPA 200.8 01/24/2013 10:10 KRICHAR

Zinc (Zn) 440 ug/L 10 10 EPA 200.8 01/24/2013 10:10 KRICHAR

2013001390

Collection Date: 18-Jan-13 8:30 AM

Site: BioReactor 1 Inf 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

ALKALINITY - (Analysis Performed by Prism Labs)

Vendor Parameter Complete Vendor Method V_PRISM

MERCURY 1631 - (Analysis Performed by Brooks Rand Labs LLC)

Vendor Parameter Complete Vendor Method V_BRAND

MERCURY 1631 - DISSOLVED - (Analysis Performed by Brooks Rand Labs LLC)

Vendor Parameter Complete Vendor Method V_BRAND

DISSOLVED METALS BY ICP

Manganese (Mn) 0.080 mg/L 0.05 10 EPA 200.7 01/28/2013 12:11 MHH7131

Page 5 of 39
Certificate of Laboratory Analysis

This report shall not be reproduced, except in full.

Order # J13010342

2013001390

Collection Date: 18-Jan-13 8:30 AM

Site: BioReactor 1 Inf 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

TOTAL RECOVERABLE METALS BY ICP

Boron (B) 15.4 mg/L 0.5 10 EPA 200.7 01/29/2013 13:38 MHH7131

Calcium (Ca) 796 mg/L 0.1 10 EPA 200.7 01/29/2013 13:38 MHH7131

Iron (Fe) < 0.1 mg/L 0.1 10 EPA 200.7 01/29/2013 13:38 MHH7131

Magnesium (Mg) 113 mg/L 0.05 10 EPA 200.7 01/29/2013 13:38 MHH7131

Manganese (Mn) 0.082 mg/L 0.05 10 EPA 200.7 01/29/2013 13:38 MHH7131

DISSOLVED METALS BY ICP-MS

Selenium (Se) 81.6 ug/L 10 10 EPA 200.8 01/24/2013 11:34 KRICHAR

TOTAL RECOVERABLE METALS BY ICP-MS

Arsenic (As) < 10 ug/L 10 10 EPA 200.8 01/24/2013 10:14 KRICHAR

Cadmium (Cd) < 10 ug/L 10 10 EPA 200.8 01/24/2013 10:14 KRICHAR

Chromium (Cr) < 10 ug/L 10 10 EPA 200.8 01/24/2013 10:14 KRICHAR

Copper (Cu) < 10 ug/L 10 10 EPA 200.8 01/24/2013 10:14 KRICHAR

Nickel (Ni) < 10 ug/L 10 10 EPA 200.8 01/24/2013 10:14 KRICHAR

Selenium (Se) 78.0 ug/L 10 10 EPA 200.8 01/24/2013 10:14 KRICHAR

Silver (Ag) < 10 ug/L 10 10 EPA 200.8 01/24/2013 10:14 KRICHAR

Zinc (Zn) < 10 ug/L 10 10 EPA 200.8 01/24/2013 10:14 KRICHAR

SELENIUM SPECIATION - (Analysis Performed by Applied Speciation and Consulting, LLC)

Vendor Parameter Complete Vendor Method V_AS&C

2013001397

Collection Date: 18-Jan-13 8:57 AM

Site: BioReactor 1 Inf BLANK 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

MERCURY 1631 - (Analysis Performed by Brooks Rand Labs LLC)

Vendor Parameter Complete Vendor Method V_BRAND

MERCURY 1631 - DISSOLVED - (Analysis Performed by Brooks Rand Labs LLC)

Vendor Parameter Complete Vendor Method V_BRAND

2013001400

Collection Date: 18-Jan-13 8:40 AM

Site: BioReactor 2 Inf 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

ALKALINITY - (Analysis Performed by Prism Labs)

Vendor Parameter Complete Vendor Method V_PRISM

Page 6 of 39
Certificate of Laboratory Analysis

This report shall not be reproduced, except in full.

Order # J13010342

2013001400

Collection Date: 18-Jan-13 8:40 AM

Site: BioReactor 2 Inf 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

MERCURY 1631 - (Analysis Performed by Brooks Rand Labs LLC)

Vendor Parameter Complete Vendor Method V_BRAND

MERCURY 1631 - DISSOLVED - (Analysis Performed by Brooks Rand Labs LLC)

Vendor Parameter Complete Vendor Method V_BRAND

DISSOLVED METALS BY ICP

Manganese (Mn) 0.091 mg/L 0.05 10 EPA 200.7 01/28/2013 12:15 MHH7131

TOTAL RECOVERABLE METALS BY ICP

Boron (B) 22.4 mg/L 0.5 10 EPA 200.7 01/29/2013 13:42 MHH7131

Calcium (Ca) 802 mg/L 0.1 10 EPA 200.7 01/29/2013 13:42 MHH7131

Iron (Fe) 0.140 mg/L 0.1 10 EPA 200.7 01/29/2013 13:42 MHH7131

Magnesium (Mg) 127 mg/L 0.05 10 EPA 200.7 01/29/2013 13:42 MHH7131

Manganese (Mn) 0.090 mg/L 0.05 10 EPA 200.7 01/29/2013 13:42 MHH7131

DISSOLVED METALS BY ICP-MS

Selenium (Se) 15.6 ug/L 10 10 EPA 200.8 01/24/2013 11:37 KRICHAR

TOTAL RECOVERABLE METALS BY ICP-MS

Arsenic (As) < 10 ug/L 10 10 EPA 200.8 01/24/2013 10:17 KRICHAR

Cadmium (Cd) < 10 ug/L 10 10 EPA 200.8 01/24/2013 10:17 KRICHAR

Chromium (Cr) < 10 ug/L 10 10 EPA 200.8 01/24/2013 10:17 KRICHAR

Copper (Cu) < 10 ug/L 10 10 EPA 200.8 01/24/2013 10:17 KRICHAR

Nickel (Ni) < 10 ug/L 10 10 EPA 200.8 01/24/2013 10:17 KRICHAR

Selenium (Se) 14.0 ug/L 10 10 EPA 200.8 01/24/2013 10:17 KRICHAR

Silver (Ag) < 10 ug/L 10 10 EPA 200.8 01/24/2013 10:17 KRICHAR

Zinc (Zn) < 10 ug/L 10 10 EPA 200.8 01/24/2013 10:17 KRICHAR

SELENIUM SPECIATION - (Analysis Performed by Applied Speciation and Consulting, LLC)

Vendor Parameter Complete Vendor Method V_AS&C

2013001401

Collection Date: 18-Jan-13 9:09 AM

Site: BioReactor 2 Inf BLANK 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

MERCURY 1631 - (Analysis Performed by Brooks Rand Labs LLC)

Vendor Parameter Complete Vendor Method V_BRAND

MERCURY 1631 - DISSOLVED - (Analysis Performed by Brooks Rand Labs LLC)

Vendor Parameter Complete Vendor Method V_BRAND

Page 7 of 39
Certificate of Laboratory Analysis

This report shall not be reproduced, except in full.

Order # J13010342

2013001402

Collection Date: 18-Jan-13 8:34 AM

Site: BioReactor 2 Eff 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

ALKALINITY - (Analysis Performed by Prism Labs)

Vendor Parameter Complete Vendor Method V_PRISM

INORGANIC IONS BY IC

Bromide 310 mg/L 5 50 EPA 300.0 01/28/2013 17:41 JAHERMA

Chloride 960 mg/L 100 1000 EPA 300.0 01/28/2013 17:41 JAHERMA

Sulfate 1500 mg/L 100 1000 EPA 300.0 01/28/2013 17:41 JAHERMA

MERCURY 1631 - (Analysis Performed by Brooks Rand Labs LLC)

Vendor Parameter Complete Vendor Method V_BRAND

MERCURY 1631 - DISSOLVED - (Analysis Performed by Brooks Rand Labs LLC)

Vendor Parameter Complete Vendor Method V_BRAND

DISSOLVED METALS BY ICP

Manganese (Mn) 0.079 mg/L 0.05 10 EPA 200.7 01/28/2013 12:19 MHH7131

TOTAL RECOVERABLE METALS BY ICP

Boron (B) 35.5 mg/L 0.5 10 EPA 200.7 01/29/2013 13:46 MHH7131

Calcium (Ca) 945 mg/L 0.1 10 EPA 200.7 01/29/2013 13:46 MHH7131

Iron (Fe) 0.740 mg/L 0.1 10 EPA 200.7 01/29/2013 13:46 MHH7131

Magnesium (Mg) 175 mg/L 0.05 10 EPA 200.7 01/29/2013 13:46 MHH7131

Manganese (Mn) 0.081 mg/L 0.05 10 EPA 200.7 01/29/2013 13:46 MHH7131

DISSOLVED METALS BY ICP-MS

Selenium (Se) 15.5 ug/L 5 5 EPA 200.8 01/24/2013 11:41 KRICHAR

TOTAL RECOVERABLE METALS BY ICP-MS

Arsenic (As) 9.90 ug/L 5 5 EPA 200.8 01/24/2013 10:20 KRICHAR

Cadmium (Cd) < 5 ug/L 5 5 EPA 200.8 01/24/2013 10:20 KRICHAR

Chromium (Cr) < 5 ug/L 5 5 EPA 200.8 01/24/2013 10:20 KRICHAR

Copper (Cu) < 5 ug/L 5 5 EPA 200.8 01/24/2013 10:20 KRICHAR

Nickel (Ni) < 5 ug/L 5 5 EPA 200.8 01/24/2013 10:20 KRICHAR

Selenium (Se) 11.9 ug/L 5 5 EPA 200.8 01/24/2013 10:20 KRICHAR

Silver (Ag) < 5 ug/L 5 5 EPA 200.8 01/24/2013 10:20 KRICHAR

Zinc (Zn) < 5 ug/L 5 5 EPA 200.8 01/24/2013 10:20 KRICHAR

SELENIUM SPECIATION - (Analysis Performed by Applied Speciation and Consulting, LLC)

Vendor Parameter Complete Vendor Method V_AS&C

Page 8 of 39
Certificate of Laboratory Analysis

This report shall not be reproduced, except in full.

Order # J13010342

2013001403

Collection Date: 18-Jan-13 9:05 AM

Site: BioReactor 2 Eff BLANK 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

MERCURY 1631 - (Analysis Performed by Brooks Rand Labs LLC)

Vendor Parameter Complete Vendor Method V_BRAND

MERCURY 1631 - DISSOLVED - (Analysis Performed by Brooks Rand Labs LLC)

Vendor Parameter Complete Vendor Method V_BRAND

2013001404

Collection Date: 18-Jan-13 12:10 PM

Site: Filter Blk 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

DISSOLVED METALS BY ICP

Manganese (Mn) < 0.005 mg/L 0.005 1 EPA 200.7 01/28/2013 11:44 MHH7131

DISSOLVED METALS BY ICP-MS

Selenium (Se) 2.01 ug/L 1 1 EPA 200.8 01/24/2013 11:17 KRICHAR

Page 9 of 39
This data package contains the analytical results for the project identified above and includes a Case Narrative, Sample 

Results and Chain of Custody. Unless otherwise noted, all samples were received in acceptable condition and processed 

according to the referenced methods. 

Data qualifiers are flagged individually on each sample. A key reference for the data qualifiers appears at the end of this case 

narrative.

NC Certification No. 402

SC Certification No. 99012

NC Drinking Water Cert No. 37735

Case Narrative

01/28/2013

Duke Energy Corporation

Jay Perkins

13339 Hagers Ferry Road

Huntersville, NC 28078

Project: Allen Wastewater - Nietering

Project No.: J13010342

Lab Submittal Date: 01/22/2013

Prism Work Order: 3010460

VA Certification No. 1287

DoD ELAP Certification No. L2307

Please call if you have any questions relating to this analytical report.

Respectfully,

PRISM LABORATORIES, INC.

VP Laboratory Services Reviewed By

Data Qualifiers Key Reference:

HT Sample received and analyzed outside of the hold time.

MDL Method Detection Limit

BRL Below Reporting Limit

RPD Relative Percent Difference

*

Results reported to the reporting limit. All other results are reported to the MDL with values between MDL and 

reporting limit indicated with a J.

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543

Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 Page 1 of 9

Page 10 of 39
Sample Receipt Summary

01/28/2013

Prism Work Order: 3010460

Client Sample ID Lab Sample ID Matrix Date Sampled Date Received

2013001388/FGD Purge Eff 3010460-01 Water 01/18/13 01/22/13

2013001390/BioReactor 1 Inf 3010460-02 Water 01/18/13 01/22/13

2013001400/BioReactor 2 Inf 3010460-03 Water 01/18/13 01/22/13

2013001402/BioReactor 2 Eff 3010460-04 Water 01/18/13 01/22/13

Samples received in good condition at 3.0 degrees C unless otherwise noted.

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543

Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 Page 2 of 9

Page 11 of 39
Laboratory Report

01/28/2013

Parameter Result Batch

ID

Report

Limit

Analysis

Date/Time

Dilution Method

Factor

Units MDL Analyst

13339 Hagers Ferry Road Project No.: 

Duke Energy Corporation Project: Allen Wastewater - Nietering

Huntersville, NC 28078

Attn: Jay Perkins

Sample Matrix: Water

Client Sample ID: 

Prism Sample ID: 3010460-01

Prism Work Order: 3010460

Time Collected: 01/18/13 10:51

Time Submitted: 01/22/13 11:00

2013001388/FGD Purge Eff

J13010342

General Chemistry Parameters

pH 7.3 HT pH Units 1 *SM4500-H B 1/22/13 14:35 JAB P3A0402

Total Alkalinity 37 mg/L 5.0 0.59 1 *SM2320 B 1/23/13 9:30 JAB P3A0411

Carbonate Alkalinity BRL mg/L 5.0 0.59 1 *SM2320 B 1/23/13 9:30 JAB P3A0412

Bicarbonate Alkalinity 37 mg/L 5.0 0.59 1 *SM2320 B 1/23/13 9:30 JAB P3A0413

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543

Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 Page 3 of 9

Page 12 of 39
Laboratory Report

01/28/2013

Parameter Result Batch

ID

Report

Limit

Analysis

Date/Time

Dilution Method

Factor

Units MDL Analyst

13339 Hagers Ferry Road Project No.: 

Duke Energy Corporation Project: Allen Wastewater - Nietering

Huntersville, NC 28078

Attn: Jay Perkins

Sample Matrix: Water

Client Sample ID: 

Prism Sample ID: 3010460-02

Prism Work Order: 3010460

Time Collected: 01/18/13 08:30

Time Submitted: 01/22/13 11:00

2013001390/BioReactor 1 Inf

J13010342

General Chemistry Parameters

pH 6.8 HT pH Units 1 *SM4500-H B 1/22/13 14:35 JAB P3A0402

Total Alkalinity BRL mg/L 5.0 0.59 1 *SM2320 B 1/23/13 9:30 JAB P3A0411

Carbonate Alkalinity BRL mg/L 5.0 0.59 1 *SM2320 B 1/23/13 9:30 JAB P3A0412

Bicarbonate Alkalinity BRL mg/L 5.0 0.59 1 *SM2320 B 1/23/13 9:30 JAB P3A0413

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543

Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 Page 4 of 9

Page 13 of 39
Laboratory Report

01/28/2013

Parameter Result Batch

ID

Report

Limit

Analysis

Date/Time

Dilution Method

Factor

Units MDL Analyst

13339 Hagers Ferry Road Project No.: 

Duke Energy Corporation Project: Allen Wastewater - Nietering

Huntersville, NC 28078

Attn: Jay Perkins

Sample Matrix: Water

Client Sample ID: 

Prism Sample ID: 3010460-03

Prism Work Order: 3010460

Time Collected: 01/18/13 08:40

Time Submitted: 01/22/13 11:00

2013001400/BioReactor 2 Inf

J13010342

General Chemistry Parameters

pH 7.2 HT pH Units 1 *SM4500-H B 1/22/13 14:35 JAB P3A0402

Total Alkalinity 70 mg/L 5.0 0.59 1 *SM2320 B 1/23/13 9:30 JAB P3A0411

Carbonate Alkalinity BRL mg/L 5.0 0.59 1 *SM2320 B 1/23/13 9:30 JAB P3A0412

Bicarbonate Alkalinity 70 mg/L 5.0 0.59 1 *SM2320 B 1/23/13 9:30 JAB P3A0413

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543

Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 Page 5 of 9

Page 14 of 39
Laboratory Report

01/28/2013

Parameter Result Batch

ID

Report

Limit

Analysis

Date/Time

Dilution Method

Factor

Units MDL Analyst

13339 Hagers Ferry Road Project No.: 

Duke Energy Corporation Project: Allen Wastewater - Nietering

Huntersville, NC 28078

Attn: Jay Perkins

Sample Matrix: Water

Client Sample ID: 

Prism Sample ID: 3010460-04

Prism Work Order: 3010460

Time Collected: 01/18/13 08:34

Time Submitted: 01/22/13 11:00

2013001402/BioReactor 2 Eff

J13010342

General Chemistry Parameters

pH 7.3 HT pH Units 1 *SM4500-H B 1/22/13 14:35 JAB P3A0402

Total Alkalinity 98 mg/L 5.0 0.59 1 *SM2320 B 1/23/13 9:30 JAB P3A0411

Carbonate Alkalinity BRL mg/L 5.0 0.59 1 *SM2320 B 1/23/13 9:30 JAB P3A0412

Bicarbonate Alkalinity 98 mg/L 5.0 0.59 1 *SM2320 B 1/23/13 9:30 JAB P3A0413

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543

Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 Page 6 of 9

Page 15 of 39
1/28/13

Level II QC Report

13339 Hagers Ferry Road Project No: J13010342

Duke Energy Corporation Project: Allen Wastewater - Nietering

Huntersville, NC 28078

Attn: Jay Perkins

Prism Work Order: 3010460

Time Submitted: 1/22/2013 11:00:00AM

Result Limit

Reporting

Units Level

Spike

Result

Source

%REC

%REC

Limits RPD

RPD

 Analyte Limit Notes 

General Chemistry Parameters - Quality Control

Batch P3A0402 - NO PREP

LCS (P3A0402-BS1) Prepared & Analyzed: 01/22/13 

pH 6.86 pH Units 6.860 100 99-101

Duplicate (P3A0402-DUP1) Source: 3010460-04 Prepared & Analyzed: 01/22/13 

pH 7.34 pH Units 7.34 0 10

Batch P3A0411 - NO PREP

Blank (P3A0411-BLK1) Prepared & Analyzed: 01/23/13 

Total Alkalinity BRL 5.0 mg/L

LCS (P3A0411-BS1) Prepared & Analyzed: 01/23/13 

Total Alkalinity 239 5.0 mg/L 250.0 96 90-110

LCS Dup (P3A0411-BSD1) Prepared & Analyzed: 01/23/13 

Total Alkalinity 239 5.0 mg/L 250.0 96 90-110 0 200

Duplicate (P3A0411-DUP1) Source: 3010460-04 Prepared & Analyzed: 01/23/13 

Total Alkalinity 102 5.0 mg/L 98.0 4 20

Batch P3A0412 - NO PREP

Blank (P3A0412-BLK1) Prepared & Analyzed: 01/23/13 

Carbonate Alkalinity BRL 5.0 mg/L

Duplicate (P3A0412-DUP1) Source: 3010460-04 Prepared & Analyzed: 01/23/13 

Carbonate Alkalinity BRL 5.0 mg/L BRL 20

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543

Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 Page 7 of 9

Page 16 of 39
1/28/13

Level II QC Report

13339 Hagers Ferry Road Project No: J13010342

Duke Energy Corporation Project: Allen Wastewater - Nietering

Huntersville, NC 28078

Attn: Jay Perkins

Prism Work Order: 3010460

Time Submitted: 1/22/2013 11:00:00AM

Result Limit

Reporting

Units Level

Spike

Result

Source

%REC

%REC

Limits RPD

RPD

 Analyte Limit Notes 

General Chemistry Parameters - Quality Control

Batch P3A0413 - NO PREP

Blank (P3A0413-BLK1) Prepared & Analyzed: 01/23/13 

Bicarbonate Alkalinity BRL 5.0 mg/L

LCS (P3A0413-BS1) Prepared & Analyzed: 01/23/13 

Bicarbonate Alkalinity 239 5.0 mg/L 250.0 96 90-110

LCS Dup (P3A0413-BSD1) Prepared & Analyzed: 01/23/13 

Bicarbonate Alkalinity 239 5.0 mg/L 250.0 96 90-110 0 200

Duplicate (P3A0413-DUP1) Source: 3010460-04 Prepared & Analyzed: 01/23/13 

Bicarbonate Alkalinity 102 5.0 mg/L 98.0 4 20

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543

Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 Page 8 of 9

Page 17 of 39
Page 9 of 9

Page 18 of 39
3958 6th Ave NW • Seattle, WA 98107 • T: 206-632-6206 • F: 206-632-6017 • www.brooksrand.com • brl@brooksrand.com

February 7, 2013

Duke Energy

ATTN: Jay Perkins

Scientific Support-Laboratory

13339 Hagers Ferry Road

Huntersville NC 28078

jcperkins@duke-energy.com

labcustomer@duke-energy.com

RE: Project DUK-HV1201 Client Project: J13010342

Dear Mr. Perkins,

On January 25, 2013, Brooks Rand Labs (BRL) received three (3) wastewater sample and one

(1) corresponding field blank. An aliquot was removed from each sample bottle and filtered into 

a separate container designed for dissolved mercury (Hg) analysis. The sample volume from 

the original container was logged-in for total Hg analysis. All samples were received, prepared, 

analyzed, and stored according to BRL SOPs and EPA methodology.

Data used for regulatory purposes has a 24 hour filtration holding time requirement. Nonregulatory purposed data has a 48 hour filtration holding time. The samples were received

outside of the non-regulatory requirement holding time and were qualified H. 

The results were blank-corrected as described in the calculations section of the relevant SOP

and may have been evaluated using reporting limits that have been adjusted to account for 

sample aliquot size. Please refer to the Sample Results page for sample-specific MDLs, MRLs, 

and other details. 

The dissolved Hg result of sample BioReactor 2 Eff Hg Blk (1304022-12) was detectable at 0.19 

ng/L and was qualified B. This concentration was less than the method defined control limit of 

0.50 ng/L however; and the associated field sample result was greater than 10x the 

concentration of the blank. Contamination was considered insignificant. All data was reported 

without additional qualification, aside from concentration qualifiers, and all associated quality 

control sample results met the acceptance criteria.

BRL, an accredited laboratory, certifies the reported results of all analyses for which BRL is 

NELAP accredited meet all NELAP requirements. For more details, see the Report Information

page of the report. Please feel free to contact us if you have any questions regarding this report.

Sincerely,

 

Tiffany Stilwater Mi Sun Um

Project Manager Data Manager

tiffany@brooksrand.com misun@brooksrand.com

Page 19 of 39
Project ID: DUK-HV1201

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Definition of Data Qualifiers

(Effective 9/23/09)

Laboratory Accreditation

BRL is accredited by the National Environmental Laboratory Accreditation Program (NELAP) through the State of Florida 

Department of Health, Bureau of Laboratories (E87982) and is certified to perform many environmental analyses. BRL is 

also certified by many other states to perform environmental analyses. For a current list of our accreditations /certifications, 

please visit our website at <http://www.brooksrand.com/default.asp?contentID=586>. Results reported relate only to the 

samples listed in the report.

Report Information

BLK

BRL

BS

CAL

CCV

CRM

D

DUP

ICV

MSD

ND

NR

PS

REC

RPD

RSD

SCV

SOP

method blank 

Brooks Rand Labs

laboratory fortified blank

calibration standard

continuing calibration verification

certified reference material

dissolved fraction

duplicate

initial calibration verification

matrix spike duplicate

non-detect

non-reportable

post preparation spike

percent recovery

relative percent difference

relative standard deviation

secondary calibration verification

standard operating procedure

MDL

MRL

MS

method detection limit

method reporting limit

matrix spike

SRM

T

COC

standard reference material

total recoverable fraction

chain of custody record 

Common Abbreviations

These qualifiers are based on those previously utilized by Brooks Rand Labs, those found in the EPA SOW ILM03.0, 

Exhibit B, Section III, pg. B-18, and the USEPA Contract Laboratory Program National Functional Guidelines for Inorganic 

Superfund Data Review; USEPA; January 2010. These supersede all previous qualifiers ever employed by BRL.

B Detected by the instrument, the result is > the MDL but ≤ the MRL. Result is reported and considered an estimate.

E An estimated value due to the presence of interferences. A full explanation is presented in the narrative.

H Holding time and/or preservation requirements not met. Result is estimated.

J Estimated value. A full explanation is presented in the narrative.

J-M Duplicate precision (RPD) for associated QC sample was not within acceptance criteria. Result is estimated.

J-N Spike recovery for associated QC sample was not within acceptance criteria. Result is estimated.

M Duplicate precision (RPD) was not within acceptance criteria. Result is estimated.

N Spike recovery was not within acceptance criteria. Result is estimated.

R Rejected, unusable value. A full explanation is presented in the narrative.

U Result is ≤ the MDL or client requested reporting limit (CRRL). Result reported as the MDL or CRRL.

X Result is not BLK-corrected and is within 10x the absolute value of the highest detectable BLK in the batch. 

Result is estimated.

Field Quality Control Samples

Please be notified that certain EPA methods require the collection of field quality control samples of an appropriate type 

and frequency; failure to do so is considered a deviation from some methods and for compliance purposes should only be 

done with the approval of regulatory authorities. Please see the specific EPA methods for details regarding required field 

quality control samples.

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Page 20 of 39
Project ID: DUK-HV1201

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Sample Information

Sample Lab ID Report Matrix Type Sampled Received

BioReactor 1 Inf 1304022-01 Influent Sample 01/18/2013 01/25/2013

BioReactor 1 Inf 1304022-02 Influent Sample 01/18/2013 01/25/2013

BioReactor 1 Inf Hg Blk 1304022-03 DIW Field Blank 01/18/2013 01/25/2013

BioReactor 1 Inf Hg Blk 1304022-04 DIW Field Blank 01/18/2013 01/25/2013

BioReactor 2 Inf 1304022-05 Influent Sample 01/18/2013 01/25/2013

BioReactor 2 Inf 1304022-06 Influent Sample 01/18/2013 01/25/2013

BioReactor 2 Inf Hg Blk 1304022-07 DIW Field Blank 01/18/2013 01/25/2013

BioReactor 2 Inf Hg Blk 1304022-08 DIW Field Blank 01/18/2013 01/25/2013

BioReactor 2 Eff 1304022-09 Effluent Sample 01/18/2013 01/25/2013

BioReactor 2 Eff 1304022-10 Effluent Sample 01/18/2013 01/25/2013

BioReactor 2 Eff Hg Blk 1304022-11 DIW Field Blank 01/18/2013 01/25/2013

BioReactor 2 Eff Hg Blk 1304022-12 DIW Field Blank 01/18/2013 01/25/2013

Batch Summary

Analyte Lab Matrix Method Prepared Analyzed Batch Sequence

Hg Water EPA 1631 01/29/2013 01/31/2013 B130128 1300071

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Page 21 of 39
Project ID: DUK-HV1201

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Sample Results

Sample Analyte Report Matrix Basis Result Qualifier MDL MRL Unit Batch Sequence

BioReactor 1 Inf

1304022-01 Hg Influent T 152 0.76 2.02 ng/L B130128 1300071

1304022-02 Hg Influent D 26.1 H 0.76 2.02 ng/L B130128 1300071

BioReactor 1 Inf Hg Blk

1304022-03 Hg DIW T 0.15 U 0.15 0.39 ng/L B130128 1300071

1304022-04 Hg DIW D 0.15 H, U 0.15 0.39 ng/L B130128 1300071

BioReactor 2 Eff

1304022-09 Hg Effluent T 38.6 0.15 0.40 ng/L B130128 1300071

1304022-10 Hg Effluent D 4.85 H 0.15 0.40 ng/L B130128 1300071

BioReactor 2 Eff Hg Blk

1304022-11 Hg DIW T 0.15 U 0.15 0.41 ng/L B130128 1300071

1304022-12 Hg DIW D 0.19 H, B 0.15 0.40 ng/L B130128 1300071

BioReactor 2 Inf

1304022-05 Hg Influent T 105 0.38 1.02 ng/L B130128 1300071

1304022-06 Hg Influent D 7.70 H 0.15 0.40 ng/L B130128 1300071

BioReactor 2 Inf Hg Blk

1304022-07 Hg DIW T 0.15 U 0.15 0.41 ng/L B130128 1300071

1304022-08 Hg DIW D 0.15 H, U 0.15 0.41 ng/L B130128 1300071

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Page 22 of 39
Project ID: DUK-HV1201

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Accuracy & Precision Summary

Batch: B130128

Analyte Native Spike Result Units REC & Limits RPD & Limits

Method: EPA 1631

Lab Matrix: Water

Sample

B130128-SRM1 Certified Reference Material (1301006, THg ICV 1641d)

Hg 15.68 16.07 ng/L 102% 85-115

B130128-MS2 Matrix Spike (1304021-05)

Hg 76.86 255.1 345.3 ng/L 105% 71-125

B130128-MSD2 Matrix Spike Duplicate (1304021-05)

Hg 76.86 255.1 345.8 ng/L 105% 71-125 240.1%

B130128-MS3 Matrix Spike (1304022-09)

Hg 38.63 53.08 93.59 ng/L 104% 71-125

B130128-MSD3 Matrix Spike Duplicate (1304022-09)

Hg 38.63 52.67 92.14 ng/L 102% 71-125 242%

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Page 23 of 39
Project ID: DUK-HV1201

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Method Blanks & Reporting Limits

Batch: B130128

Method: EPA 1631

Matrix: Water

Analyte: Hg

Sample Result Units

B130128-BLK1 0.35 ng/L

B130128-BLK2 0.43 ng/L

B130128-BLK3 0.24 ng/L

B130128-BLK4 0.25 ng/L

Average: 0.32 Standard Deviation: 0.09 MDL: 0.15

Limit: 0.50 Limit: 0.10 MRL: 0.40

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Page 24 of 39
Project ID: DUK-HV1201

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Instrument Calibration

Sequence: 1300071

Instrument: THG-05 Method: EPA 1631

Date: 01/31/2013

Total Mercury and Mercury Speciation by CVAFS

Analyte: Hg

Lab ID True Value Result Units REC & Limits

1300071-IBL1 1.14 pg of Hg

1300071-IBL2 2.63 pg of Hg

1300071-IBL3 3.17 pg of Hg

1300071-IBL4 3.35 pg of Hg

1300071-CAL1 10.00 10.27 pg of Hg 103%

1300071-CAL2 25.00 25.06 pg of Hg 100%

1300071-CAL3 100.0 98.35 pg of Hg 98%

1300071-CAL4 500.0 492.3 pg of Hg 98%

1300071-CAL5 2500 2478 pg of Hg 99%

1300071-CAL6 10000 10130 pg of Hg 101%

1300071-ICV1 1568 1607 pg of Hg 102% 85-115

1300071-CCB1 7.92 pg of Hg

1300071-CCV1 500.0 502.1 pg of Hg 100% 77-123

1300071-CCB2 5.03 pg of Hg

1300071-CCB3 5.29 pg of Hg

1300071-CCB4 4.31 pg of Hg

1300071-CCV2 500.0 500.9 pg of Hg 100% 77-123

1300071-CCB5 6.92 pg of Hg

1300071-CCV3 500.0 500.9 pg of Hg 100% 77-123

1300071-CCB6 4.05 pg of Hg

1300071-CCV4 500.0 511.4 pg of Hg 102% 77-123

1300071-CCB7 9.42 pg of Hg

1300071-CCV5 500.0 533.0 pg of Hg 107% 77-123

1300071-CCB8 7.74 pg of Hg

1300071-CCV6 500.0 532.6 pg of Hg 107% 77-123

1300071-CCB9 8.66 pg of Hg

1300071-CCV7 500.0 536.4 pg of Hg 107% 77-123

1300071-CCBA 8.76 pg of Hg

1300071-CCV8 500.0 526.2 pg of Hg 105% 77-123

1300071-CCBB 5.55 pg of Hg

1300071-CCV9 500.0 529.6 pg of Hg 106% 77-123

1300071-CCBC 6.39 pg of Hg

1300071-CCVA 500.0 524.4 pg of Hg 105% 77-123

1300071-CCBD 5.13 pg of Hg

1300071-CCVB 500.0 529.8 pg of Hg 106% 77-123

1300071-CCBE 5.65 pg of Hg

1300071-CCVC 500.0 523.0 pg of Hg 105% 77-123

1300071-CCBF 5.54 pg of Hg

1300071-CCVD 500.0 523.6 pg of Hg 105% 77-123

1300071-CCBG 4.88 pg of Hg

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Page 25 of 39
Project ID: DUK-HV1201

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Instrument Calibration

Sequence: 1300071

Instrument: THG-05 Method: EPA 1631

Date: 01/31/2013

Total Mercury and Mercury Speciation by CVAFS

Analyte: Hg

Lab ID True Value Result Units REC & Limits

1300071-CCVE 500.0 523.6 pg of Hg 105% 77-123

1300071-CCBH 3.67 pg of Hg

1300071-CCVF 500.0 516.9 pg of Hg 103% 77-123

1300071-CCBI 4.48 pg of Hg

1300071-CCVG 500.0 509.2 pg of Hg 102% 77-123

1300071-CCBJ 3.59 pg of Hg

1300071-CCVH 500.0 525.4 pg of Hg 105% 77-123

1300071-CCBK 5.51 pg of Hg

1300071-ICV2 1568 1672 pg of Hg 107% 85-115

1300071-CCVI 500.0 509.8 pg of Hg 102% 77-123

1300071-CCBL 4.04 pg of Hg

1300071-CCVJ 500.0 531.6 pg of Hg 106% 77-123

1300071-CCBM 4.48 pg of Hg

1300071-CCVK 500.0 531.4 pg of Hg 106% 77-123

1300071-CCBN 4.38 pg of Hg

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Page 26 of 39
Project ID: DUK-HV1201

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Sample Containers

Lab ID: 1304022-01 Report Matrix: Influent

Sample: BioReactor 1 Inf Sample Type: Sample Received: 01/25/2013

Collected: 01/18/2013

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 76663301

0

Bottle FLPE Hg-T 500mL none n/a Cooler

Lab ID: 1304022-02 Report Matrix: Influent

Sample: BioReactor 1 Inf Sample Type: Sample Received: 01/25/2013

Collected: 01/18/2013

Comments: Qualify H

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A Bottle FLPE Hg-T 250mL 13-0001 none n/a Cooler

Lab ID: 1304022-03 Report Matrix: DIW

Sample: BioReactor 1 Inf Hg Blk Sample Type: Field Blank Received: 01/25/2013

Collected: 01/18/2013

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 76663301

0

Bottle FLPE Hg-T 500mL none n/a Cooler

Lab ID: 1304022-04 Report Matrix: DIW

Sample: BioReactor 1 Inf Hg Blk Sample Type: Field Blank Received: 01/25/2013

Collected: 01/18/2013

Comments: Qualify H

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A Bottle FLPE Hg-T 250mL 13-0001 none n/a Cooler

Lab ID: 1304022-05 Report Matrix: Influent

Sample: BioReactor 2 Inf Sample Type: Sample Received: 01/25/2013

Collected: 01/18/2013

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 76663301

0

Bottle FLPE Hg-T 500mL none n/a Cooler

Lab ID: 1304022-06 Report Matrix: Influent

Sample: BioReactor 2 Inf Sample Type: Sample Received: 01/25/2013

Collected: 01/18/2013

Comments: Qualify H

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A Bottle FLPE Hg-T 250mL 13-0001 none n/a Cooler

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Page 27 of 39
Project ID: DUK-HV1201

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Sample Containers

Lab ID: 1304022-07 Report Matrix: DIW

Sample: BioReactor 2 Inf Hg Blk Sample Type: Field Blank Received: 01/25/2013

Collected: 01/18/2013

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 76663301

0

Bottle FLPE Hg-T 500mL none n/a Cooler

Lab ID: 1304022-08 Report Matrix: DIW

Sample: BioReactor 2 Inf Hg Blk Sample Type: Field Blank Received: 01/25/2013

Collected: 01/18/2013

Comments: Qualify H

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A Bottle FLPE Hg-T 250mL 13-0001 none n/a Cooler

Lab ID: 1304022-09 Report Matrix: Effluent

Sample: BioReactor 2 Eff Sample Type: Sample Received: 01/25/2013

Collected: 01/18/2013

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 76663301

0

Bottle FLPE Hg-T 500mL none n/a Cooler

Lab ID: 1304022-10 Report Matrix: Effluent

Sample: BioReactor 2 Eff Sample Type: Sample Received: 01/25/2013

Collected: 01/18/2013

Comments: Qualify H

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A Bottle FLPE Hg-T 250mL 13-0001 none n/a Cooler

Lab ID: 1304022-11 Report Matrix: DIW

Sample: BioReactor 2 Eff Hg Blk Sample Type: Field Blank Received: 01/25/2013

Collected: 01/18/2013

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 76663301

0

Bottle FLPE Hg-T 500mL none n/a Cooler

Lab ID: 1304022-12 Report Matrix: DIW

Sample: BioReactor 2 Eff Hg Blk Sample Type: Field Blank Received: 01/25/2013

Collected: 01/18/2013

Comments: Qualify H

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A Bottle FLPE Hg-T 250mL 13-0001 none n/a Cooler

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Page 28 of 39
Project ID: DUK-HV1201

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Shipping Containers

Cooler

Tracking No: 535305197910 via FedEx

Temperature: 0.9 °C

Coolant Type: Ice

Description: Cooler

Damaged in transit? No

Returned to client? No

Custody seals present? Yes

Custody seals intact? Yes

COC present? Yes

Received: January 25, 2013 8:30

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Page 29 of 39
Page 30 of 39
 

February 7, 2013 

Jay Perkins 

Duke Energy Analytical Laboratory 

Mail Code MGO3A2 (Building 7405) 

13339 Hagers Ferry Rd. 

Huntersville, NC 28078 

(704) 875-5245 

Project: Allen Wastewater - Nietering (January 2013 - Test Burn) (LIMS #J13010342) 

Dear Mr. Perkins, 

Attached is the report associated with four (4) aqueous samples submitted for selenium 

speciation on January 24, 2013. The samples were received in a sealed cooler at -0.1°C on 

January 25, 2013. Selenium speciation analysis was performed via ion chromatography 

inductively coupled plasma collision reaction cell mass spectrometry (IC-ICP-CRC-MS). Any 

issues associated with the analysis are addressed in the following report. 

If you have any questions, please feel free to contact me at your convenience. 

 Sincerely, 

 

 Russell Gerads 

Vice President 

Applied Speciation and Consulting, LLC 

 

 

Page 31 of 39
Applied Speciation and Consulting, LLC 

Report prepared for: 

Jay Perkins 

Duke Energy Analytical Laboratory 

Mail Code MGO3A2 (Building 7405) 

13339 Hagers Ferry Rd. 

Huntersville, NC 28078 

Project: Allen Wastewater - Nietering (January 2013 - Test Burn) (LIMS #J13010342) 

February 7, 2013 

1. Sample Reception 

Four (4) aqueous samples in 125mL HDPE bottles (provided by Applied Speciation and 

Consulting) were submitted for selenium speciation analysis on January 24, 2013. The 

samples were received on January 25, 2013 in a sealed container at -0.1°C. 

The samples were received in a laminar flow clean hood, void of trace metals contamination 

and ultra-violet radiation, and were designated discrete sample identifiers. An aliquot of 

each sample was filtered (0.45μm) and each filtrate was stored in a secure, monitored 

cryofreezer (maintained at a temperature of -80oC) until selenium speciation analysis could 

be performed via ion chromatography inductively coupled plasma collision reaction cell 

mass spectrometry (IC-ICP-CRC-MS). 

2. Sample Preparation 

All sample preparation is performed in laminar flow clean hoods known to be free from trace 

metals contamination. All applied water for dilutions and sample preservatives are 

monitored for contamination to account for any biases associated with the sample results. 

Selenium Speciation Analysis by IC-ICP-CRC-MS Prior to analysis, an aliquot of each 

sample was filtered with a syringe filter (0.45μm) and injected directly into an autosampler 

vial. No further sample preparation was performed as any chemical alteration of a sample 

may shift the equilibrium of the system, resulting in changes in speciation ratios. 

3. Sample Analysis 

All sample analysis is preceded by a minimum of a five-point calibration curve spanning the 

entire concentration range of interest. Calibration curves are performed at the beginning of 

Page 32 of 39
each analytical day. All calibration curves, associated with each species of interest, are 

standardized by linear regression resulting in a response factor. All sample results are 

instrument blank corrected to account for any operational biases associated with the 

analytical platform. 

Prior to sample analysis, all calibration curves are verified using second source standards 

which are identified as initial calibration verification standards (ICV). 

Ongoing instrument performance is identified by the analysis of continuing calibration 

verification standards (CCV) and continuing calibration blanks (CCB) at a minimum interval 

of every ten analytical runs. 

Selenium Speciation Analysis by IC-ICP-CRC-MS Each sample for selenium speciation 

analysis was analyzed by ion chromatography inductively coupled plasma collision reaction 

cell mass spectrometry (IC-ICP-CRC-MS) on January 30, 2013. An aliquot of each sample 

is injected onto an anion exchange column and mobilized by a basic (pH > 7) gradient. The 

eluting selenium species are then introduced into a radio frequency (RF) plasma where 

energy-transfer processes cause desolvation, atomization, and ionization. The ions are 

extracted from the plasma through a differentially-pumped vacuum interface and travel 

through a pressurized chamber (CRC) containing a reaction gas which preferentially reacts 

with interfering ions of the same target mass to charge ratios (m/z). A solid-state detector 

detects ions transmitted through the mass analyzer and the resulting current is processed by a 

data handling system. 

Retention times for each eluting species are compared to known standards for species 

identification. 

4. Analytical Issues 

The overall analyses went well and no significant analytical issues were encountered. All 

quality control parameters associated with the samples were within acceptance limits. 

The estimated method detection limits (eMDLs) for selenite, selenate, and selenocyanate are 

generated from replicate analyses of the lowest standard in the calibration curve. Not all 

selenium species are present in preparation blanks; therefore, eMDL calculations based on 

preparation blanks are artificially biased low. 

The eMDL for methylseleninic acid and selenomethionine is calculated from the average 

eMDL of selenite, selenate, and selenocyanate. The calibration does not contain 

methylseleninic acid or selenomethionine due to impurities in these standards which would 

bias the results for other selenium species. 

Page 33 of 39
If you have any questions or concerns regarding this report, please feel free to contact me. 

Sincerely, 

Russell Gerads 

Vice President 

Applied Speciation and Consulting, LLC 

Page 34 of 39
Sample Results

Sample ID Se(IV) Se(VI) SeCN MeSe(IV) SeMe

Unknown Se 

Species (n)

FGD Purge Eff 499 86.4 ND (<5.2) ND (<6.3) ND (<6.3) 0.0 (0)

BioReactor 1 Inf 27.3 15.1 ND (<1.3) ND (<1.6) ND (<1.6) 0.0 (0)

BioReactor 2 Inf 2.5 ND (<0.95) ND (<1.3) ND (<1.6) ND (<1.6) 0.0 (0)

BioReactor 2 Eff ND (<2.5) ND (<0.95) ND (<1.3) ND (<1.6) ND (<1.6) 0.0 (0)

All results reflect the applied dilution and are reported in μg/L

ND = Not detected at the applied dilution

SeCN = Selenocyanate

MeSe(IV) = Methylseleninic acid

SeMe = Selenomethionine

Unknown Se Species = Total concentration of all unknown Se species observed by IC-ICP-MS

Report Generated by: Russell Gerads

Applied Speciation and Consulting, LLC

Selenium Speciation Results for Duke Energy

Project Name: Allen Wastewater - Nietering (January 2013 - Test Burn)

Contact: Jay Perkins

Date: February 7, 2013

LIMS #J13010342

Page 35 of 39
Quality Control Summary - Preparation Blank Summary

Analyte (μg/L) PBW1 PBW2 PBW3 PBW4 Mean StdDev eMDL* eMDL 250x eMDL 1000x

Se(IV) 0.000 0.000 0.000 0.000 0.000 0.000 0.010 2.5 9.8

Se(VI) 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.95 3.8

SeCN 0.000 0.000 0.000 0.000 0.000 0.000 0.005 1.3 5.2

MeSe(IV) 0.000 0.000 0.000 0.000 0.000 0.000 0.006 1.6 6.3

SeMe 0.000 0.000 0.000 0.000 0.000 0.000 0.006 1.6 6.3

eMDL = Estimated Method Detection Limit

*Please see narrative regarding eMDL calculations

Quality Control Summary - Certified Reference Materials

Analyte (μg/L) CRM True Value Result Recovery

Se(IV) LCS 9.57 9.64 100.8

Se(VI) LCS 9.48 9.01 95.1

SeCN LCS 8.92 8.50 95.2

MeSe(IV) LCS 6.47 6.09 94.2

SeMe LCS 9.32 8.58 92.0

Applied Speciation and Consulting, LLC

Selenium Speciation Results for Duke Energy

Project Name: Allen Wastewater - Nietering (January 2013 - Test Burn)

Contact: Jay Perkins

LIMS #J13010342

Date: February 7, 2013

Report Generated by: Russell Gerads

Page 36 of 39
Quality Control Summary - Matrix Duplicates

Analyte (μg/L) Sample ID Rep 1 Rep 2 Mean RPD

Se(IV) Batch QC 5.80 5.27 5.5 9.7

Se(VI) Batch QC ND (<0.95) ND (<0.95) NC NC

SeCN Batch QC ND (<1.3) ND (<1.3) NC NC

MeSe(IV) Batch QC ND (<1.6) ND (<1.6) NC NC

SeMe Batch QC ND (<1.6) ND (<1.6) NC NC

ND = Not detected at the applied dilution

NC = Value was not calculated due to one or more concentrations below the eMDL

Quality Control Summary - Matrix Spike/ Matrix Spike Duplicate

Analyte (μg/L) Sample ID Spike Conc MS Result Recovery Spike Conc MSD Result Recovery RPD

Se(IV) Batch QC 1390 1272 91.1 1390 1256 90.0 1.3

Se(VI) Batch QC 1261 1049 83.2 1261 1031 81.8 1.7

SeCN Batch QC 1144 879.6 76.9 1144 867.2 75.8 1.4

Applied Speciation and Consulting, LLC

Selenium Speciation Results for Duke Energy

Project Name: Allen Wastewater - Nietering (January 2013 - Test Burn)

Contact: Jay Perkins

LIMS #J13010342

Date: February 7, 2013

Report Generated by: Russell Gerads

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