Maternal Statistics / Treatment Group

KEY: S#,M# Gp# = code for blind treatments

TWtGN = Total Weight gain during pregnancy#Fet = number of fetuses in the litter#Res = number of resoprtion sites (no fetus) in the uterine horn#Imp = number of implantation sites (no fetus)in the uterine hornN Hrt = number of fetuses with Normal HeartsAbnHrt = number of fetuses with Abnormal HeartsAbnormalites = the type of cardiac defect seen

1100 ppm Trichloroethylene - Pregnancy Only Drinking waterS# M# Gp# TWtGn #Fet #Res #Imp N Hrt AbnHrt Abnormalities

6 73 16 121 16 0 1 16 06 74 16 106 8 2 0 7 1 1-ASD, VSD6 75 16 139 14 0 0 13 1 1-ASD6 76 16 158 20 1 0 17 3 1-A-V canal(VSD); 2-ASD; 3-ASD6 77 16 120 10 2 1 10 06 78 16 122 15 0 0 13 2 1-ASD; 2-AorticV-unicuspid, VSD6 80 16 94 7 1 0 6 1 1-AorticV-fenestrated ant. leaflet6 81 16 94 3 0 0 3 0

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6 81 16 94 3 0 0 3 06 82 16 134 12 5 0 9 3 1-ASD, VSD; 2-VSD-two muscular; 3-ASD

Stats 9 121 105 11 2 94 11

1.5 ppm Trichloroethylene - Pregnancy only Drinking waterS# M# Gp# TWtGn #Fet #Res #Imp N Hrt AbnHrt Abnormalities

6 85 17 96 4 0 0 4 06 86 17 154 14 1 0 14 06 88 17 109 13 1 0 13 06 89 17 143 14 0 0 14 06 90 17 127 16 0 0 16 06 92 17 158 15 0 0 13 2 1-L transposition; 2-VSD-subaortic6 93 17 127 16 0 0 15 1 1-Absent RPA,PA and AO parallel,small PA6 94 17 136 14 0 0 14 06 96 17 183 17 0 0 14 3 1-ASD; 2-ASD; 3-VSD6 97 17 116 11 1 0 9 2 1-VSD-apical and muscular; 2-ASD9 4 17 135 13 3 0 13 09 11 17 134 17 0 0 17 09 33 17 142 17 0 0 16 1 1-ASD

Stats 13 135 181 6 0 172 9

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Maternal Statistics / Treatment Group

250 ppb Trichloroethylene - Drinking water, Pregnancy OnlyS# M# Gp# TWtGn #Fet #Res #Imp N Hrt AbnHrt Abnormalities

20 1 53 124 15 1 0 14 1 1-TriV -small (R8 and R9 twinned-N Hrts)20 5 53 128 10 1 0 10 020 6 53 139 12 1 0 11 1 1-Huge Coronary Sinus 20 7 53 125 13 1 0 11 2 1-AoV-dysplastic, no coronary; 2-Tri/MitrV-abn20 8 53 155 17 0 0 17 020 11 53 166 14 6 0 14 020 12 53 167 16 0 0 16 020 13 53 61 1 0 0 1 020 23 53 False pregnancy20 19 53 116 12 1 0 11 1 1-ASD 

Stats 10 131.22 110 11 0 105 5

2.5 ppb TCE in Pregnancy Only Drinking WaterS# M# Gp# TWtGn #Fet #Res #Imp N Hrt AbnHrt Abnormalities

22 8 52 127 11 0 0 11 0

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22 8 52 127 11 0 0 11 022 10 52 140 13 2 0 13 022 12 52 137 13 1 0 13 022 13 52 115 10 2 0 10 022 14 52 125 14 0 0 14 022 15 52 134 13 1 0 13 022 17 52 120 14 1 0 14 022 20 52 108 12 1 0 12 022 21 52 107 7 0 0 7 022 40 52 127 13 0 0 13 022 43 52 118 12 2 0 12 022 45 52 128 12 0 0 12 0

Stats 12 123.83 144 10 0 144 0

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Maternal Statistics / Treatment Group

Control S# M# Gp# #Fet N Hrt AbnHrt Abnormalities

4 4 0.2 14 11 3 1- VSD, ECD-vent.attachmts, 2- D-loop in Rt Chest, 3- D-loop in Rt Chest4 24 0.2 12 11 1 1- VSD9 23 0.2 3 2 1 1- VSD-hi subAO9 26 0.2 12 11 1 1- ASD9 28 0.2 9 8 1 1- VSD lg muscular

17 16 0.2 13 12 1 1-ASD, Mitravl V-leaflet atresia19 55 0.2 17 15 2 1-ASD, 2-ASD20 15 0.2 14 12 2 1-ASD, 2-ASD, large23 16 0.2 13 12 1 1-ASD

4 1 0.2 11 11 04 10 0.2 10 10 04 12 0.2 15 15 04 14 0.2 11 11 04 22 0.2 19 19 05 5 0.2 11 11 05 15 0.2 11 11 05 21 0.2 12 12 0

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5 21 0.2 12 12 06 84 0.2 4 4 06 109 0.2 12 12 09 2 0.2 5 5 09 3 0.2 14 14 09 5 0.2 14 14 09 12 0.2 13 13 09 27 0.2 17 17 0

12 8 0.2 15 15 017 8 0.2 16 16 017 19 0.2 9 9 017 24 0.2 12 12 019 10 0.2 8 8 019 25 0.2 7 7 019 35 0.2 5 5 019 43 0.2 9 9 019 57 0.2 5 5 019 59 0.2 12 12 020 18 0.2 4 4 020 20 0.2 8 8 020 22 0.2 10 10 021 11 0.2 13 13 0

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Maternal Statistics / Treatment Group

22 1 0.2 12 12 022 6 0.2 9 9 022 9 0.2 12 12 022 18 0.2 13 13 022 31 0.2 11 11 022 44 0.2 12 12 023 1 0.2 11 11 023 3 0.2 12 12 023 6 0.2 16 16 023 19 0.2 15 15 023 21 0.2 2 2 023 23 0.2 12 12 023 30 0.2 1 1 023 31 0.2 12 12 023 33 0.2 14 14 023 35 0.2 10 10 023 36 0.2 13 13 055 606 593 13

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55 litters total 606 Fetuses total

9 litters with fetuses with abnormal hearts out of the 55 total litters

13 Fetuses with abnormal hearts in 9 Litters

593 Normal Fetuses in 46 Litters

Control Summary (No statistical sign between groups over time, so controls were thus combined)606 fetuses /55 maternal rats total 9 litters with fetuses with abnormal hearts out of the 55 total litters13/606 fetuses with abnormal hearts

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