### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX- V9360) 75WG and

·

Nicosulfuron 75WG plus Isodecylalcohol Ethoxylated (IN-KG691) Surfactant to Aquatic

Vascular Plants, 

### Lemna gibba G3

PMRA Submission Number {

........ } EPA MRID Number 48354308

Data Requirement: PMRA OAT A CODE

EPA DP Barcode

OECD Data Point

EPAMRID

EPA Guideline {



### ............ }

386877

{



## ............ }

48354308

850.4400

Test material: Nicosulfuron (DPX-V9360) 75WG and Nicosulfuron 75WG plus Isodecylalcohol Ethoxylated

(IN-KG691) Surfactant

Purity: 75% ai (for DPX360-119)

Common name: Nicosulfuron

ChemiCal name: IUPAC

CAS name: 2-[[[[ ( 4,6-Dimethoxy-2-pyrimidinyl)amino ]carbonyl]amino ]sulfonyl]- N ,N-dimethyl3-pyridinecarboxamide monohydrate (for nicosulfuron (DPX-V9360 active ingredient); Poly(oxy1,2-ethanediyl), .alpha.-isodecyl-.omega.-hydroxy- (for surfactant IN-KG691)

CAS No.: 111991-09-4 (for nicosulfuron active ingredient), 78330-20-8 (for the surfactant INKG691)

Synonyms: Nicosulfuron 75WG plus INKG691 Surfactant, DPX-V9360 75WG, DPX-V9360-119,

Nicosulfuron (DPX- V9360) 75WG, Nicosulfuron 75WG, 3-Pyridinecarboxamide, 2-[[[[(4,6-

dimethoxy-2-pyrimidinyl)amino ]carbonyl]amino ]sulfony 1]- N,N-dimethyl, monohydrate (inverted

CAS name for nicosulfuron active ingredient), DPX-KG691, DPX-KG-010, Flo Mo Low Foam A,

IN-Kg691, IN-KG691-010

Primary Reviewer: Mia Howard

Staff Scientist, Cambridge Environmental, Inc.

Secondary Reviewer: Teri S. Myers

Environmental Scientist, CDM Smith

Primary Reviewer: Geoffrey Sinclair, Biologist

EPA/OPP/EFED/ERB-5

Secondary Reviewer(s): {

...................................... }

{EP A/OECD/PMRA}

Reference/Submission No.: {.

.................... }

Company Code

Active Code

Use Site Category:

EPA PC Code {



### ............ }

{



### ............ }

{



## ............ }

129008 [ForPMRA]

[For PMRA]

[For PMRA]

Date Evaluation Completed: {dd-mm-yyyy} Signature:

# ~fir>~

Date: 10/03/12

Signature:**~,.** 

## '5 

# mu

..**/L.<o~** Date: 08/14/13

## ~ "}"/ ./

Date: 9/18/13

# #~~/'J.

Date: {

............ }

CITATION: Sloman, T.L. 2004. Nicosulfuron (DPX-V9360) 75WG and Nicosulfuron 75WG plus Isodecylalchol

Ethoxylated (IN-KG691) Surfactant: Influence on Growth and Reproduction of Lemna gibba G3. An

Page 1 of46

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) 75WG and

Nicosulfuron 75WG plus Isodecylalcohol Ethoxylated (IN

- KG691) Surfactant to Aquatic

Vascular Plants, Lemna gibba G3

PMRA Submission Number 

### {........} EPA MRID Number 48354308

Page 1 of 46 Data Requirement: PMRA DATA CODE {............}

EPA DP Barcode 386877

OECD Data Point {............}

EPA MRID 48354308

EPA Guideline 850.4400

Test material: Nicosulfuron (DPX

- V9360) 75WG and Nicosulfuron 75WG plus Isodecylalcohol Ethoxylated

(IN

- KG691) Surfactant

Purity: 75% ai (for DPX360

- 119)

Common name : Nicosulfuron

Chemical name: IUPAC

CAS name : 2

- [[[[(4,6

- Dimethoxy

- 2

- pyrimidinyl)amino]carbonyl]amino]sulfonyl]

- N,N

- dimethyl

3

- pyridinecarboxamide monohydrate (for nicosulfuron (DPX

- V9360 active ingredient); Poly(oxy

1,2

- ethanediyl), .alpha.

- isodecyl

- .omega.

- hydroxy

- (for surfactant IN

- KG691)

CAS No. : 111991

- 09

- 4 (for nicosulfuron active ingredient), 78330

- 20

- 8 (for the surfactant IN

KG691)

Synonyms : Nicosulfuron 75WG plus INKG691 Surfactant, DPX

- V9360 75WG, DPX

- V9360

- 119,

Nicosulfuron (DPX

- V9360) 75WG, Nicosulfuron 75WG, 3

- Pyridinecarboxamide, 2

- [[[[(4,6

- dimethoxy

- 2

- pyrimidinyl)amino]carbonyl]amino]sulfonyl]

- N,N

- dimethyl, monohydrate (inverted

CAS name for nicosulfuron active ingredient), DPX

- KG691, DPX

- KG

- 010, Flo Mo Low Foam A,

IN

- Kg691, IN

- KG691

- 010

Primary Revi ewer: Mia Howard Signature:

Staff Scientist, Cambridge Environmental, Inc. Date: 10/03/12

Secondary Reviewer: Teri S. Myers Signature:

Environmental Scientist, CDM Smith Date: 08/14 /13

Primary Reviewer: Geoffrey Sinclair, Biologist Date: 9 / 18 /13

EPA/ OPP/EFED/ERB

- 5

Secondary Reviewer(s): {......................................} Date: {............}

{EPA/OECD/PMRA}

Reference/Submission No.: {.....................}

Company Code {............} [For PMRA]

Active Code {............} [For PMRA]

Use Site Category: {............} [For PMRA]

EPA PC Code 129008

Date Evaluation Completed: {dd

- mm

- yyyy}

CITATION : Sloman, T.L. 2004. Nicosulfuron (DPX

- V9360) 75WG and Nicosulfuron 75WG plus Isodecylalchol

Ethoxylated (IN

- KG691) Surfactant: Influence on Growth and Reproduction of Lemna gibba G3. An

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) 75WG and

Nicosulfuron 75WG plus Isodecylalcohol Ethoxylated (IN

- KG691) Surfactant to Aquatic

Vascular Plants, Lemna gibba G3

PMRA Submission Number 

### {........} EPA MRID Number 48354308

Page 2 of 46 unpublished study performed by E.I. du Pont de Nemours and Company, Haskell Laboratory for Health and

Environmental Sciences, Newark, Delaware. Laboratory projec t ID DuPont

- 12112. Study sponsored by E.I. du

Pont de Nemours and Company, Wilmington, Delaware. Study initiated 04 August 2003 and completed 30

September 2004.

DISCLAIMER : This document provides guidance for EPA and PMRA reviewers on how to complete a data

evaluation record after reviewing a scientific study concerning the acute toxicity of a pesticide to aquatic vascular

plants. It is not intended to prescribe conditions to any external party for conducting this study nor to establish

absolute criteri a regarding the assessment of whether the study is scientifically sound and whether the study satisfies

any applicable data requirements. Reviewers are expected to review and to determine for each study, on a case

- by

case basis, whether it is scientifical ly sound and provides sufficient information to satisfy applicable data

requirements. Studies that fail to meet any of the conditions may be accepted, if appropriate; similarly, studies that

meet all of the conditions may be rejected, if appropriate. In sum, the reviewer is to take into account the totality of

factors related to the test methodology and results in determining the acceptability of the study.

EXECUTIVE SUMMARY :

In two 14

- day acute toxicity studies , the freshwater floating aquatic vascular plants, duckweed ( Lemna gibba G3)

were exposed to Nicosulfuron 75WG at nominal concentrations of 0 (negative control), 0.5, 1, 2, 4, and 8 μg/L

under static conditions. The nominal active ingredient concentrations of nicosulfuron were 0 (negative control), 0.5,

1, 2, 4, and 8 μg ai/L. In the first study, the plants were exposed to Nicosulfuron 75WG only, and in the second

study the plants were additionally exposed to IN

- KG691 surfactant. Nominal concentrations of the surfactant in the

second stu dy were 0 (negative control), 1.825, 3.75, 7.5, 15, and 30 μg/L. M ean measured concentrations of

n icosulfuron in the first study were <0.0037 (<LOD, negative control), 0.424, 0.789, 1.690, 3.300, and 6.440 μg

ai/L. Mean measured concentrations of nicosul furon in the second study were <0.0032 (<LOD, negative control),

0.389, 0.737, 1.520, 5.225, and 8.135 μg ai/L.

In the Nicosulfuron 75WG study, t he NOAEC and EC50/IC50 v alues based on frond number (the most sensitive

endpoint) were 1.690 and 2.6 μ g a.i /L, respectively. T he NOAEC and EC50/IC50 v alues based on 0

- 7 day growth

rate were 0.789 and 3.5 μ g a.i/L, respectively

. T he NOAEC and EC50/IC50 v alues based on 0

- 14 day growth rate

were 1.690 and 4.1 μ g a.i/L, respectively

. T he NOAEC and EC50/IC50 v alu es based on biomass were 0.789 and

3.2 μ g a.i/L, respectively

. The % growth inhibition of frond number in the treated culture as compared to the control

ranged from

- 5 to 91%.

In the Nicosulfuron 75WG plus IN

- KG691 surfactant study, t he NOAEC and EC50 /IC50 v alues based on frond

number were 0.737 and 3.0 μ g a.i/L, respectively. T he NOAEC and EC50/IC50 v alues based on 0

- 7 day growth

rate were 0.737 and 4.9 μ g a.i/L, respectively

. T he NOAEC and EC50/IC50 v alues based on 0

- 14 day growth rate

were 0.737 a nd 5.0 μ g a.i/L, respectively

. T he NOAEC and EC50/IC50 v alues based on biomass were 0.737 and

4.5 μ g a.i/L, respectively

. The % growth inhibition of frond number in the treated culture as compared to the control

ranged from 0 to 84%.

The following abnormalities were noted: chlorosis and necrosis.

This toxicity study is classified as scientifically sound or and satisfies the guideline requirement for an aquatic plant

( Lemna gibba G3) toxicity study. It is therefore classified as accep table.

Results Synopsis

Test Organism: Duckweed, Lemna gibba G3

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) 75WG and

Nicosulfuron 75WG plus Isodecylalcohol Ethoxylated (IN

- KG691) Surfactant to Aquatic

Vascular Plants, Lemna gibba G3

PMRA Submission Number 

### {........} EPA MRID Number 48354308

Page 3 of 46 Test Type (Flow

- through, Static, Static Renewal): Static

Nicosulfuron 75WG study

Frond number

EC 05 : 1.1 μg ai/L 95% C.I.: 0.77 to 1.5 μg ai/L

EC 50 : 2.6 μg ai/L 95% C.I.: 2.3 to 3.1 μg ai/L

NOAEC: 1.690 μg ai/L

Probit Slope: 4.23 ± 0.492

Growth rate (0

- 7 days)

EC 05 : 0.60 μg ai/L 95% C.I.: 0.31 to 1.2 μg ai/L

EC 50 : 3.5 μg ai/L 95% C.I.: 2.9 to 4.4 μg ai/L

NOAEC: 0.789 μg ai/L

Probit Slope: 2.14 ± 0.31 1

Growth rate (0

- 14 days)

EC 05 : 1.3 μg ai/L 95% C.I.: 1.0 to 1.6 μg ai/L

EC 50 : 4.1 μg ai/L 95% C.I.: 3.8 to 4.5 μg ai/L

NOAEC: 1.690 μg ai/L

Probit Slope: 3.21 ± 0.239

Biomass

EC 05 : 0.58 μg ai/L 95% C.I.: 0.27 to 1.3 μg ai/L

EC 50 : 3.2 μg ai /L 95% C.I.: 2.5 to 4.2 μg ai/L

NOAEC: 0.789 μg ai/L

Probit Slope: 2.21 ± 0.360

Endpoint(s) Effected: frond number, 0

- 7 day growth rate, 0

- 14 day growth rate, biomass

Nicosulfuron 75WG plus IN

- KG691 surfactant study

Frond number

EC 05 : 1.1 μg ai/L 95% C.I.: 0.85 to 1.3 μg ai/L

EC 50 : 3.0 μg ai/L 95% C.I.: 2.7 to 3.3 μg ai/L

NOAEC: 0.737 μg ai/L

Probit Slope: 3.64 ± 0.213

Growth rate (0

- 7 days)

EC 05 : 0.84 μg ai/L 95% C.I.: 0.54 to 1.3 μg ai/L

EC 50 : 4.9 μg ai/L 95% C.I.: 4.4 to 5.6 μg ai /L

NOAEC: 0.737 μg ai/L

Probit Slope: 2.14 ± 0.210

Growth rate (0

- 14 days)

EC 05 : 1.6 μg ai/L 95% C.I.: 1.2 to 2.1 μg ai/L

EC 50 : 5.0 μg ai/L 95% C.I.: 4.6 to 5.4 μg ai/L

NOAEC: 0.737 μg ai/L

Probit Slope: 3.30 ± 0.295

Biomass

EC 05 : 0.69 μg ai /L 95% C.I.: 0.40 to 1.2 μg ai/L

EC 50 : 4.5 μg ai/L 95% C.I.: 3.8 to 5.3 μg ai/L

NOAEC: 0.737 μg ai/L

Probit Slope: 2.03 ± 0.221

Endpoint(s) Effected: frond number, 0

- 7 day growth rate, 0

- 14 day growth rate, biomass

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) 75WG and

Nicosulfuron 75WG plus Isodecylalcohol Ethoxylated (IN

- KG691) Surfactant to Aquatic

Vascular Plants, Lemna gibba G3

PMRA Submission Number 

### {........} EPA MRID Number 48354308

Page 4 of 46 I. MATERIALS AND METHODS

GUIDELINE FOLLOWED: The study was designed to comply with the U.S. Environmental Protection

Agency (EPA) Pesticide Assessment Guidelines Subdivision J, 122

- 2, 123

- 2 (1982). The following deviations from the U.S. EPA OCSPP 850.4400

(2012) guidelines are n oted:

1. The storage conditions of the test substance were not reported.

2. The age of the inoculum at test initiation was not reported. An age of 7

- 12 days is recommended.

3. The pH and total organic carbon, particulate matter, metal, pesticide, and chlorine contents of the

dilution water were not reported.

4. Based on 0

- 14 day growth rates, the doubling times of frond number were 2.84 and 2.78 days for the

Nicosulfuron 75WG and Nicosulfuron 75WG plus IN

- KG691 studies, respectively. The validity

criteria of the OCSPP 850.4400 guidelines state that the doubling time of frond number must not

exceed 2.5 days. However, based on 0

- 7 day growth rates, the doubling times of frond number were

2.14 and 1.96 days for the Nicosulfuron 75WG and Nicosulfuron 75WG plus IN

- KG6 91 st udies,

respectively, meeting the validity criterion.

5. Three replicates were used per level for biomass measurements. A minimum of four replicates per test

level are recommended. Four replicates were used for the measurement of all other endpoints.

T hese deviations do not affect the validity of the study.

COMPLIANCE: Signed and dated GLP, Quality Assurance and Data Confidential ity

statements were provided. The study was conducted in compliance with the

US EPA FIFRA (40 CFR part 160) GLP Standards with one exception: the

individual components of the Nicosulfuron 75WG plus Isodecylalcohol

Ethoxylated (IN

- KG691) Surfactant blend used as the test substance were

characterized, but no individual characterization was performed on the

blend itself.

A. MATERIALS:

1. Test material Nicosulfuron (DPX

- V9360) 75WG and Nicosulfuron 75WG plus

Isodecylalcohol Ethoxylated (IN

- KG691) Surfactant

Description: Nicosulfur on (DPX

- V9360) is described as white (colorless) crystals. The

IN

- KG691 surfactant i s described as a clear liquid.

Lot No./Batch No. : DPX

- V9360

- 119 and IN

- KG691

- 010

Purity: 75% ai (for DPX360

- 119), Nicosulfuron 75WG plus In

- KG691 Surfactant

is 1 part Nicosulfuron 75WG and 3.75 parts IN

- KG691 surfactant

Stability of compound under test conditions: In the Nicosulfuron 75WG only study, initial nicosulfuron

concentrations ranged from 94 to 102% of nominal. Final nicosulfuron concentrations ranged from

112 to 131% of nominal. Final measured concentrations ranged from 110 to 140% of initial measured

concentrations. In the Nicosulfuron 75WG plus IN

- KG691 surfactant study, initial measured

concentrations ranged from 95 to 176% of nominal. Final measured concentrations ranged from 99 to

173% of nominal. Final measured concentrations ranged from 96 to 106% of initial measured

concentrations.

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) 75WG and

Nicosulfuron 75WG plus Isodecylalcohol Ethoxylated (IN

- KG691) Surfactant to Aquatic

Vascular Plants, Lemna gibba G3

PMRA Submission Number 

### {........} EPA MRID Number 48354308

Page 5 of 46 (OECD recommends water solubility, stability in water and light, pKa, Pow, and vapor pr essure of test

compound)

Storage conditions of

test chemicals: Not reported.

Physicochemical properties of nicosulfuron.

Parameter Values Comments

Water solubility at 20 Ε C Not reported Solubility in 20X

AAP is 172 mg/L at

22°C

Vapor pressure Not reported

UV absorption Not reported

pKa Not reported

Kow Not reported

2. Test organism :

Name: Duckweed, Lemna gibba G3 EPA requires a vascular species: Lemna gibba

. Strain, if provided: G3

Source: In

- house cultures originally obtained from Dr. Janet P. Sloven, Ph.D, USDA/ARS,

Horticulture Crop Quality Lab, Beltsville, Maryland

Age of inoculum : Not reported

Method of cultivation: Cultured and tested in 20X AAP nutrient medium, transferred to fresh medium

every 7

- 14 days

B. STUDY DESIGN:

1. Experimental Conditions

a. Range

- finding study . A range

- finding study was used to select exposure concentrations for the

definitive test.

b. Definitive Study

Table 1: Experimental Parameters

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) 75WG and

Nicosulfuron 75WG plus Isodecylalcohol Ethoxylated (IN

- KG691) Surfactant to Aquatic

Vascular Plants, Lemna gibba G3

PMRA Submission Number 

### {........} EPA MRID Number 48354308

Page 6 of 46 Parameter Details Remarks

Criteria

Acclimation period:

Culturing media and conditions:

(same as test or not)

Health: (any mortality observed) Continuous

Same as test

None reported

Test system

Static/static renewal

Renewal rate for static renewal Static

N/A EPA expects the test concentrations to

be renewed every 3 to 4 days (one

renewal for the 7 day test, 3

- 4 renewals

for the 14 day test).

Incubation facility Environmental chamber

Duration of the test 14 days

EPA requires a duration of 14 days.

Seven day studies will be accepted for

review by the Agency.

Test vessel

Material: (glass/stainless steel)

Size:

Fill volume: Not reported

250 mL

100 mL Canted neck flasks

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) 75WG and

Nicosulfuron 75WG plus Isodecylalcohol Ethoxylated (IN

- KG691) Surfactant to Aquatic

Vascular Plants, Lemna gibba G3

PMRA Submission Number 

### {........} EPA MRID Number 48354308

Page 7 of 46 Parameter Details Remarks

Criteria

Details of 20X AAP medium

( Nicosulfuron 75WG study)

pH at test initiation:

pH at test termination:

(Nicosulfuron 75WG plus IN

KG691 surfactant study)

pH at test initiation:

pH at test termination:

Chelator used:

Carbon source: 7.60 to 8.05

8.82 to 9.66

6.95 to 7.46

8.88 to 9.44

Na 2 EDTAx2H 2 O

NaHCO 3 EPA recommends the following

culture media: Modified

Hoagland’s E+ or 20X

- AAP.

C

helating agents (e.g. EDTA) are

recommended in the nutrient

medium for optimum cell growth.

Lower concentrations of chelating

agents (down to one

- third of the

normal concentration recommended

for AAP medium) may be used in the

nutrient medium used for test

solution preparation if it is

suspected that the chelator will

interact with the test material.

ASTM reference, E14 15

- 91and D

3978

- 80 (reapproved 1987).

If non

- standard nutrient medium

was used, detailed composition

provided (Yes/No) A standard medium was used and a

detailed composition was also

provided

Dilution water

source/type:

pH:

water pretreatment (if any):

Total Organic Carbon:

particulate matter:

metals:

pesticides:

chlorine: Milli

- Q (deionized) water

Not reported

Deionized

Not reported

Not reported

Not reported

Not reported

Not reported EPA recommends a pH of ~5.0. A

solution pH of 7.5 is acceptable if type

20X

- AAP nutrient media is used.

Indicate how the test material is

added to the medium (added

directly or used stock solution) Used stock solutions prepared in

20X AAP medium.

Aeration or agitation N/A

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) 75WG and

Nicosulfuron 75WG plus Isodecylalcohol Ethoxylated (IN

- KG691) Surfactant to Aquatic

Vascular Plants, Lemna gibba G3

PMRA Submission Number 

### {........} EPA MRID Number 48354308

Page 8 of 46 Parameter Details Remarks

Criteria

Sediment used ( for rooted aquatic

vascular plants )

Origin:

Textural classification (%sand, silt,

and clay):

Organic carbon (%):

Geographic location: N/A

Number of replicates

Control:

Solvent control:

Treatments: 4

N/A

4 A single abiotic control was used for

each test

Only 3 replicates were used for

biomass measurements

Number of plants/replicate 5

EPA requires 5 plants.

Number of fronds/plant 3

EPA requires 3 fronds per plant.

Test concentrations

(Nicosulfuron 75WG study)

Nominal Nicosulfuron 75WG : 0 (negative control), 0.5, 1, 2, 4, 8

μg/L The mean measured nicosulfuron

(active ingredient) concentrations

were calculated by the reviewer as

the arithmetic mean of the measured

concentrations at days 0 and 14.

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) 75WG and

Nicosulfuron 75WG plus Isodecylalcohol Ethoxylated (IN

- KG691) Surfactant to Aquatic

Vascular Plants, Lemna gibba G3

PMRA Submission Number 

### {........} EPA MRID Number 48354308

Page 9 of 46 Parameter Details Remarks

Criteria

Nominal nicosulfuron (active

ingredient):

M ean m easured nicosulfuron

(active ingredient) :

( Nicsulfuron 75WG plus IN

KG691 surfactant study)

Nominal Nicosulfuron 75WG:

Nominal nicosulfuron (active

ingredient):

Mean measured nicosulfuron

(active ingredient):

Nominal IN

- KG691: 0 (negative control), 0.5, 1, 2, 4, 8

μg/L

<0.0037 (<LOD, negative control),

0.376, 0.751, 1.50, 3.00, 6.01 μg

ai/L

0 (negative control), 0.5, 1, 2, 4, 8

μg/L

0 (negative control), 0.5, 1, 2, 4, 8

μg/L

<0.0032 (<LOD, negative control),

0.389, 0.737, 1.520, 5.225, 8.135

0 (negative control), 1.875, 3.75,

7.5, 15, 30 μg/L EPA requires at least 5 test

concentrations with a dose range of 2X

or 3X progression.

Solvent (type, percentage, if used) N/A

Method and interval of analytical

verification The concentration of nicosulfuron

was measured on days 0 and 14

using LC/MS/MS.

Test conditions

Temperature (Nicosulfuron 75WG

study):

Temperature (Nicosulfuron 75WG

plus IN

- KG691 surfactant study) :

Photoperiod:

Light intensity and quality: 25.1 to 25.3°C

24.1 to 24.5°C

Continuous

4360 to 5180 lux (Nicosulfuron

75WG study),

---- (Nicosulfuron

75WG plus IN

- KG691 surfactant

study), warm

- white fluorescent light

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) 75WG and

Nicosulfuron 75WG plus Isodecylalcohol Ethoxylated (IN

- KG691) Surfactant to Aquatic

Vascular Plants, Lemna gibba G3

PMRA Submission Number 

### {........} EPA MRID Number 48354308

Page 10 of 46 Parameter Details Remarks

Criteria

Reference chemical (if used)

name:

concentrations: N/A

Other parameters, if any N/A

2. Observations:

Table 2: Observation parameters

Parameters Details Remarks/Criteria

Parameters measured (e.g.,:

number of fronds, plant dry weight

or other toxicity symptoms) Frond number

Biomass

Growth rate

Measurement technique for frond

number and other end points The number of healthy fronds

was determined by direct

counting using an illuminated

magnifier

. Plants were dried for

approximately 48 hours at

approximately 70°C to

determine the dry weight for

biomass measurements. The

growth rate was determined

using a logarithmic equation

based on frond number.

Observation intervals Fronds were counted on days 0,

2, 7, 9, 12, and 14

Other observations, if any 14

- day recovery tests were

performed subsequent to the

definitive tests.

Indicate whether there was an

exponential growth in the control Based on 0

- 7 day growth rates,

the doubling times of frond

number were 2.14 and 1.96 days

for the Nicosulfuron 75WG and

Nicosulfuron 75WG plus IN

KG691 studies, respectively.

However, based on 0

- 14 day

growth rates, the doubling times

were 2.84 and 2.78 days for the Doubling times were calculated by the

reviewer (see Reviewer’s Comments

section)

The OCSPP 850.4400 guidelines state

that the doubling time must not exceed

2.5 days.

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) 75WG and

Nicosulfuron 75WG plus Isodecylalcohol Ethoxylated (IN

- KG691) Surfactant to Aquatic

Vascular Plants, Lemna gibba G3

PMRA Submission Number 

### {........} EPA MRID Number 48354308

Page 11 of 46 Parameters Details Remarks/Criteria

Nicosulfuron 75WG and

Nicosulfuron 75WG plus IN

KG691 studies, respectively.

Were raw data included? Yes

II. RESULTS and DISCUSSION:

A. INHIBITORY EFFECTS:

Nicosulfuron 75WG study

After 14 days, the mean frond number of the negative control was 459, yielding inhibitions relative to the

negative control of

- 5,

- 4,

- 4, 76, and 91% for nominal Nicosulfuron 75WG concentrations of 0.5, 1, 2, 4, and 8

μg/L, respectively. The NOAEC and EC50 values calculated by the study author based on nominal

Nicosulfuron 75WG concentrations were2 and 3.26 μg/L, respectively.

The mean 0

- 7 day growth rate of the negative control was 0.3235 /day , yielding inhibitions relative to the

negative c ontrol of

- 3,

- 3, 11, 58 and 64% for nominal Nicosulfuron 75WG concentrations of 0.5, 1, 2, 4, and 8

μg/L, respectively. The NOAEC and EC50 values calculated by the study author based on nominal

Nicosulfuron 75WG concentrations were 2 and 3.41μg/L, respec tively.

The mean 0

- 14 day growth rate of the negative control was 0.2444 /day , yielding inhibitions relative to the

negative control of

- 1,

- 1,

- 1 ,42 and 71% for nominal Nicosulfuron 75WG concentrations of 0.5, 1, 2, 4, and 8

μg/L, respectively. The NOAE C and EC50 values calculated by the study author based on nominal

Nicosulfuron 75WG concentrations were 4 and 5.07 μg/L, respectively.

After 14 days, the mean biomass of the negative control was 43.5 mg, yielding inhibitions relative to the

negative contr ol of

- 3, 0, 13, 63, and 68% for nominal Nicosulfuron 75WG concentrations of 0.5, 1, 2, 4, and 8

μg/L, respectively. The NOAEC and EC50 values calculated by the study author based on nominal

Nicosulfuron 75WG concentrations were 1 and 3.29μg/L, respective ly.

Chlorotic and necrotic fronds were observed at all test levels on day 14. There was an increase in pH during the

study.

Nicosulfuron 75WG plus IN

- KG691 surfactant study

After 14 days, the mean frond number of the negative control was 489, yielding inhibitions relative to the

negative control of 0, 2, 4, 8, and 84% for nominal Nicosulfuron 75WG concentrations of 0.5, 1, 2, 4, and 8

μg/L, respectively. The NOAEC and EC50 v alues calculated by the study author based on nominal

Nicosulfuron 75WG concentrations were 1 and 3.22 μg/L, respectively.

The mean 0

- 7 day growth rate of the negative control was 0.3538/day, yielding inhibitions relative to the

negative control of 1, 0, 5, 56, and 65% for nominal Nicosulfuron 75WG concentrations of 0.5, 1, 2, 4, and 8

μg/L, respectively. The NOAEC and EC50 values calculated by the study author based on nominal

Nicosulfuron 75WG concentrations were 1 and 3.70 μg/L, respectively.

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) 75WG and

Nicosulfuron 75WG plus Isodecylalcohol Ethoxylated (IN

- KG691) Surfactant to Aquatic

Vascular Plants, Lemna gibba G3

PMRA Submission Number 

### {........} EPA MRID Number 48354308

Page 12 of 46 The mean 0

- 14 day growth rate of the negative control was 0.2477/day, yielding inhibitions relative to the

negative control of

- 1, 0, 2, 53, and 75 % for nominal Nicosulfuron 75WG concentrations of 0.5, 1, 2, 4, and 8

μg/L, respectively. The NOAEC and EC50 values calculated by the study author based on nominal

Nicosulfuron 75WG concentrations were 1 and 3.92μg/L, respectively.

After 14 days, the mean biomass of the negative control was 41.6 mg, yielding inhibitions relative to the

negative control of 0, 0, 13, 58 and 67 % for nominal Nicosulfuron 75WG concentrations of 0.5, 1, 2, 4, and 8

μg/L, respectively. The NOAEC and EC50 values calculated by the study author based on nominal

Nicosulfuron 75WG concentrations were 1 and 3.49μg/L, respectively.

Chlorotic and necrotic fronds were observed at all test levels on day 14. There was an increase in pH during the

study.

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) 75WG and

Nicosulfuron 75WG plus Isodecylalcohol Ethoxylated (IN

- KG691) Surfactant to Aquatic

Vascular Plants, Lemna gibba G3

PMRA Submission Number 

### {........} EPA MRID Number 48354308

Page 13 of 46 Ta ble 3: Effect of Nicosulfuron 75WG on frond number of ( duckweed, Lemna gibba G3 )

Treatment

Nominal

Nicosulfuron

75WG (and

nominal

nicosulfuron

active ingredient)

[and mean

measured

nicosulfuron

active ingredient]

concentrations

(μg /L) Initial frond

number/test

solution frond number at

2 days 7 days 14 days

frond number % inhibition

Negative control 15 28 145 459 N/A

0.5 (0.376) [0.424] 15 27 157 481

- 5

1 (0.751) [0.789] 15 28 156 477

- 4

2 (1.5) [1.690] 15 25 113 477

- 4

4 (3.00) [3.300] 15 22 39 108 76

8 (6.01) [6.400] 15 23 34 41 91

Reference chemical

(if used) N/A

Table 4: Effect of Nicosulfuron 75WG on frond number of (duckweed, Lemna gibba G3)

Treatment

Nominal

Nicosulfuron 75WG

(and nominal

nicosulfuron active

ingredient) [and

mean measured

nicosulfuron active

ingredient]

concentrations

(μg/L) Growth rate,

0

- 7 days (per

day) %

inhibition* Growth rate,

0

- 14 days

(per day) %

inhibition* Biomass

(mg) %

inhibition*

Negative control 0.3235 N/A 0.2444 N/A 43.7 N/A

0.5 (0.376) [0.424] 0.3347

- 3 0.2477

- 1 44.9

- 3

1 (0.751) [0.789] 0.3340

- 3 0.2471

- 1 43.7 0

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) 75WG and

Nicosulfuron 75WG plus Isodecylalcohol Ethoxylated (IN

- KG691) Surfactant to Aquatic

Vascular Plants, Lemna gibba G3

PMRA Submission Number 

### {........} EPA MRID Number 48354308

Page 14 of 46 2 (1.5) [1.690] 0.2878 11 0.2471

- 1 37.9 13

4 (3.00) [3.300] 0.1343 58 0.1410 42 16.3 63

8 (6.01) [6.400] 0.1163 64 0.0720 71 13.8 68

* Calculated by the reviewer relative to the negative control, based on mean values

Table 5: Statistical endpoint values for the Nicosulfuron 75WG study.* (calculated by the study author using

nominal Nicosulfuron 75WG concentrations)

Statistical Endpoint Frond number (14

days) Growth rate (0

- 7

days) Growth rate (0

- 14

days) Biomass (14 days)

NOAEC (μg/L) 2 2 4 1

IC 50 or EC 50 (μg/L) (95% C.I.) 3.26 (2.93 to 3.64) 3.41 (3.09 to 3.72) 5.07 (4.54 to 5.65) 3.29 (2.84 to 3.75)

Reference chemical

NOAEC

IC 50 /EC 50 N/A

* Do not use this table, if the study was deemed unacceptable.

Table 6 : Effect of Nicosulfuron 75WG plus IN

- KG691 on frond number of (duckweed, Lemna gibba G3)

Treatment

Nominal

Nicosulfuron

75WG (and

nominal

nicosulfuron

active ingredient)

[and mean

measured

nicosulfuron

active ingredient]

{and IN

- KG691

surfactant}

concentrations

(μg/L) Initial frond

number/test

solution frond number at

2 days 7 days 14 days

frond number % inhibition

Negative control 15 40 179 489 N/A

0.5 (0.376) [0.389]

{1.875} 15 32 473 491 0

1 (0.751) [0.737]

{3.75} 15 35 178 478 2

2 (1.5) [1.520] 15 31 141 449 4

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) 75WG and

Nicosulfuron 75WG plus Isodecylalcohol Ethoxylated (IN

- KG691) Surfactant to Aquatic

Vascular Plants, Lemna gibba G3

PMRA Submission Number 

### {........} EPA MRID Number 48354308

Page 15 of 46 {7.5}

4 (3.00) [5.225]

{15} 15 32 45 78 8

8 (6.01) [8.135]

{30} 15 29 36 36 84

Reference chemical

(if used) N/A

Table 7 : Effect of Nicosulfuron 75WG plus IN

- KG691 on frond number of (duckweed, Lemna gibba G3)

Treatment

Nominal

Nicosulfuron 75WG

(and nominal

nicosulfuron active

ingredient) [and

mean measured

nicosulfuron active

ingredient] {and IN

KG691 surfactant}

concentrations

(μg/L) Growth rate,

0

- 7 days (per

day) %

inhibition* Growth rate

(per day) %

inhibition* Biomass %

inhibition*

Negative control 0.3538 N/A 0.2477 N/A 41.6 N/A

0.5 (0.376) [0.389]

{1.875} 0.3493 1 0.2491

- 1 41.8 0

1 (0.751) [0.737]

{3.75} 0.3527 0 0.2472 0 41.7 0

2 (1.5) [1.520] {7.5} 0.3364 5 0.2427 2 36.1 13

4 (3.00) [5.225] {15} 0.1563 56 0.1173 53 17.3 58

8 (6.01) [8.135] {30} 0.1231 65 0.0616 75 13.9 67

* Calculated by the reviewer relative to the negative control, based on mean values

Table 8 : Statistical endpoint values for the Nicosulfuron 75WG plus IN

- KG691 surfactant study.*

(calculated by the study author using nominal Nicosulfuron 75WG concentrations)

Statistical Endpoint Frond number (14

days) Growth rate (0

- 7

days) Growth rate (0

- 14

days) Biomass (14 days)

NOAEC (μg/L) 1 1 1 1

IC 50 or EC 50 (μg/L) (95% C.I.) 3.22 (3.11 to 3.32) 3.7 0 (3.48 to 3.93) 3.92 ( 3.86 to 3.99 ) 3.49 (3.04 to 3.94)

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) 75WG and

Nicosulfuron 75WG plus Isodecylalcohol Ethoxylated (IN

- KG691) Surfactant to Aquatic

Vascular Plants, Lemna gibba G3

PMRA Submission Number 

### {........} EPA MRID Number 48354308

Page 16 of 46 Reference chemical

NOAEC

IC 50 /EC 50 N/A

* Do not use this table, if the study was deemed unacceptable.

B. REPORTED STATISTICS:

The study author statistically analyzed the endpoints for frond number , 0

- 7 day growth rate, 0

- 14 day growth

rate, and biomass using nominal exposure concentrations. The EC50 values were determined using inverse

weighted least

- squares non

- linear regression. The EC 50 values based on frond number were calculated using

probit analysis. The NOAEC values were determined using Dunnett’s tests , for the Nicosulfuron 75WG study

, and Jonkheere tests, for the Nicosulfuron 75WG plus IN

- KG691 surfactant study

.

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) 75WG and

Nicosulfuron 75WG plus Isodecylalcohol Ethoxylated (IN

- KG691) Surfactant to Aquatic

Vascular Plants, Lemna gibba G3

PMRA Submission Number 

### {........} EPA MRID Number 48354308

Page 17 of 46 C. VERIFICATION OF STATISTICAL RESULTS:

Statistical Method: The reviewer statistically analyzed the endpoints for frond number, 0

- 7 day growth rate, 0

- 14 day growth rate, and biomass using mean measured nicosulfuron (active ingredient) concentrations. The

data were assessed for normality and homogeneity of variance using Shapiro

- Wilk’s and Levene’s tests,

respectively. All data met all assumptions of normality and homogeneity of variance without transformation,

except for the 0

- 14 day growth rate data for the Nicosulfuron 75WG plus IN

- KG691 surfactant study, which

met the assumptions of homogeneity of va riance, but not normality. These data required an optimal power

transformation with power= 1.99 to meet the assumptions of normality. The NOAEC values we re calculated

using ANOVA followed by William’s tests. All preceding analyses were performed using Toxstat statistical

software. The ECx values were determined using probit analysis via Nuthatch statistical software.

Nicosulfuron 75WG study

Frond numbe r

EC 05 : 1.1 μg ai/L 95% C.I.: 0.77 to 1.5 μg ai/L

EC 50 : 2.6 μg ai/L 95% C.I.: 2.3 to 3.1 μg ai/L

NOAEC: 1.690 μg ai/L

Probit Slope: 4.23 ± 0.492

Growth rate (0

- 7 days)

EC 05 : 0.60 μg ai/L 95% C.I.: 0.31 to 1.2 μg ai/L

EC 50 : 3.5 μg ai/L 95% C.I.: 2.9 to 4.4 μg ai/L

NOAEC: 0.789 μg ai/L

Probit Slope: 2.14 ± 0.311

Growth rate (0

- 14 days)

EC 05 : 1.3 μg ai/L 95% C.I.: 1.0 to 1.6 μg ai/L

EC 50 : 4.1 μg ai/L 95% C.I.: 3.8 to 4.5 μg ai/L

NOAEC: 1.690 μg ai/L

Probit Slope: 3.21 ± 0.239

Biomass

EC 05 : 0.58 μg ai/L 95% C.I.: 0.27 to 1.3 μg ai/L

EC 50 : 3.2 μg ai/L 95% C.I.: 2.5 to 4.2 μg ai/L

NOAEC: 0.789 μg ai/L

Probit Slope: 2.21 ± 0.360

Nicosulfuron 75WG plus IN

- KG691 surfactant study

Frond number

EC 05 : 1.1 μg ai/L 95% C.I.: 0.85 to 1.3 μg ai/L

EC 50 : 3.0 μg ai/L 95% C.I.: 2.7 to 3.3 μg ai/L

NOAEC: 0.737 μg ai/L

Probit Slope: 3.64 ± 0.213

Growth rate (0

- 7 days)

EC 05 : 0.84 μg ai/L 95% C.I.: 0.54 to 1.3 μg ai/L

EC 50 : 4.9 μg ai/L 95% C.I.: 4.4 to 5.6 μg ai/L

NOAEC: 0.737 μg ai/L

Probit Slope: 2.14 ± 0.210

Growth rate (0

- 14 days)

EC 05 : 1.6 μg ai/L 95% C.I.: 1.2 to 2.1 μg ai/L

EC 50 : 5.0 μg ai/L 95% C.I.: 4.6 to 5.4 μg ai/L

NOAEC: 0.737 μg ai/L

Probit Slope: 3.30 ± 0.295

Biomass

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) 75WG and

Nicosulfuron 75WG plus Isodecylalcohol Ethoxylated (IN

- KG691) Surfactant to Aquatic

Vascular Plants, Lemna gibba G3

PMRA Submission Number 

### {........} EPA MRID Number 48354308

Page 18 of 46 EC 05 : 0.69 μg ai/L 95% C.I .: 0.40 to 1.2 μg ai/L

EC 50 : 4.5 μg ai/L 95% C.I.: 3.8 to 5.3 μg ai/L

NOAEC: 0.737 μg ai/L

Probit Slope: 2.03 ± 0.221

D. STUDY DEFICIENCIES:

There were not any major deficiencies noted in this study.

E. REVIEWER ’ S COMMENTS:

The results of the reviewer were similar to those of the study author. The study author used nominal

formulation concentrations to perform all analyses, whereas the reviewer used mean measured active ingredient

concentrations. The statistical methods of the study autho r and the reviewer also differed. Therefore, the

results of the reviewer and the study author differ slightly.

The reviewer calculated the doubling time of the control treatments based on both 0

- 7 and 0

- 14 day growth

rates of frond number using the follo wing equation:



### T

d 

### = ln(2) / r control

Where 

### T d = doubling time in days and 

### r control = mean growth rate of the control in 1/days

Based on 0

- 7 day growth rates, both studies would meet the validity criterion of a doubling time <2.5 days.

Based on 0

- 14 day growth rate, the doubling time would exceed the validity criterion stated in the OCSPP

850.4400 guidelines.

The study author noted that the high recoveries of the nicosulfuron active ingredient in the Nicosulfuron 75WG

plus IN

- KG691 study may have bee n due to an interaction with the surfactant

.

F. CONCLUSIONS:

This study is scientifically and meets the guideline requirements for toxicity study with the aquatic plant Lemna

gibba . It is thus classified as acceptable

. In the Nicosulfuron 75WG study, the most sensitive endpoint was

frond number , with NOAEC and EC50 values of 1.690 and 2.6 μg ai/L, respectively. In the Nicosulfuron

75WG plus IN

- KG691 surfactant study, the most sensitive endpoint was frond number, with NOAEC and EC50

v alues of 0.737 and 3.0 μg ai/L, respectively.

Nicosulfuron 75WG study

Frond number

EC 05 : 1.1 μg ai/L 95% C.I.: 0.77 to 1.5 μg ai/L

EC 50 : 2.6 μg ai/L 95% C.I.: 2.3 to 3.1 μg ai/L

NOAEC: 1.690 μg ai/L

Probit Slope: 4.23 ± 0.492

Growth rate (0

- 7 days)

EC 05 : 0.60 μg ai/L 95% C.I.: 0.31 to 1.2 μg ai/L

EC 50 : 3.5 μg ai/L 95% C.I.: 2.9 to 4.4 μg ai/L

NOAEC: 0.789 μg ai/L

Probit Slope: 2.14 ± 0.311

Growth rate (0

- 14 days)

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) 75WG and

Nicosulfuron 75WG plus Isodecylalcohol Ethoxylated (IN

- KG691) Surfactant to Aquatic

Vascular Plants, Lemna gibba G3

PMRA Submission Number 

### {........} EPA MRID Number 48354308

Page 19 of 46 EC 05 : 1.3 μg ai/L 95% C.I.: 1.0 to 1.6 μg ai/L

EC 50 : 4.1 μg ai/L 95% C.I .: 3.8 to 4.5 μg ai/L

NOAEC: 1.690 μg ai/L

Probit Slope: 3.21 ± 0.239

Biomass

EC 05 : 0.58 μg ai/L 95% C.I.: 0.27 to 1.3 μg ai/L

EC 50 : 3.2 μg ai/L 95% C.I.: 2.5 to 4.2 μg ai/L

NOAEC: 0.789 μg ai/L

Probit Slope: 2.21 ± 0.360

Endpoint(s) Effected : frond number, 0

- 7 day growth rate, 0

- 14 day growth rate, biomass

Nicosulfuron 75WG plus IN

- KG691 surfactant study

Frond number

EC 05 : 1.1 μg ai/L 95% C.I.: 0.85 to 1.3 μg ai/L

EC 50 : 3.0 μg ai/L 95% C.I.: 2.7 to 3.3 μg ai/L

NOAEC: 0.737 μg ai/L

Probit Slope: 3.64 ± 0.213

Growth rate (0

- 7 days)

EC 05 : 0.84 μg ai/L 95% C.I.: 0.54 to 1.3 μg ai/L

EC 50 : 4.9 μg ai/L 95% C.I.: 4.4 to 5.6 μg ai/L

NOAEC: 0.737 μg ai/L

Probit Slope: 2.14 ± 0.210

Growth rate (0

- 14 days)

EC 05 : 1.6 μg ai/L 95% C.I .: 1.2 to 2.1 μg ai/L

EC 50 : 5.0 μg ai/L 95% C.I.: 4.6 to 5.4 μg ai/L

NOAEC: 0.737 μg ai/L

Probit Slope: 3.30 ± 0.295

Biomass

EC 05 : 0.69 μg ai/L 95% C.I.: 0.40 to 1.2 μg ai/L

EC 50 : 4.5 μg ai/L 95% C.I.: 3.8 to 5.3 μg ai/L

NOAEC: 0.737 μg ai/ L

Probit Slope: 2.03 ± 0.221

Endpo int(s) Effected: frond number, 0

- 7 day growth rate, 0

- 14 day growth rate, biomass

III. REFERENCES :

1. DuPont Haskell Laboratory (2003). Solubility of Nicosulfuron (DPX

- V9360) 75WG in

20X AAP Nutrient Medium. Unpublished report, DuPont

- 12889.

2. American Society for Testing and Materials (ASTM). (1991). "Standard Guide for

Conducting Static Toxicity Tests with Lemna gibba G3" in ASTM Annual Book of Standards,

E1415

- 91. Vol. 11.04, Philadelphia, PA.

3. Levene, H . (1960). Robust test for equality of variances. Contributions to Probability and

Statistics (J. Olkin, ed.), 278

- 292. Stanford University Press, Palo Alto.

4. Snedecor, G.W. and Cochran, W.G. (1967). Statistical Methods, 6th edition. The Iowa State

Univer sity Press, Ames.

5. Bruce, R. D. and Versteeg, D. J. (1992). A statistical procedure for modeling continuous

toxicity data. Environ. Toxicol. Chem. 11:1485

- 1494.

6. Hochberg, Y. and Tamhane, A. C. (1987). Multiple Comparison Procedures, Wiley, New

York.

7 . Jonckheere, A. R. (1954). A distribution

- free fc

- sample test against ordered alternatives,

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) 75WG and

Nicosulfuron 75WG plus Isodecylalcohol Ethoxylated (IN

- KG691) Surfactant to Aquatic

Vascular Plants, Lemna gibba G3

PMRA Submission Number 

### {........} EPA MRID Number 48354308

Page 20 of 46 Biometrika41, 133.

8. SAS Software Version 8.2, SAS Institute, Gary, NC, 1999.

9. Slob, W. (2002). Dose

- Response Modeling of Continuous Endpoints, Toxicological

Sciences 66, 298

- 312.

10. Terpstra, T. J. (1952). The Asymptotic Normality and Consistency of Kendall's Test

Against Trend, When Ties are Present in One Ranking, Indag. Math. 14, 327

- 333.

11. U.S.EPA. (1995). The Use of the Benchmark Dose Approach in Healt h Risk Assessment,

Risk Assessment Forum, EPA/630/R

- 94/007, United States Environmental Protection

Agency, Washington, D.C. Principal authors: Crump, K., Allen, B., and Faustman, E.

12. Westfall, P.H., Tobias, R.D., Rom, D., Wolfinger, R.D., and Hochberg, Y. (1999). Multiple

comparisons and multiple tests, SAS Institute, Cary, North Carolina.

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) 75WG and

Nicosulfuron 75WG plus Isodecylalcohol Ethoxylated (IN

- KG691) Surfactant to Aquatic

Vascular Plants, Lemna gibba G3

PMRA Submission Number 

### {........} EPA MRID Number 48354308

Page 21 of 46 APPENDIX I. OUTPUT OF THE REVIEWER’S STATISTICAL VERIFICATION

NICOSULFURON 75WG STUDY

FROND NUMBER

Title: NICOSULFURON LEMNA 14 DAY FROND NUMBER

File: 4308F Transform: NO

TRANSFORMATION

Shapiro

- Wilk's Test for Normality

---------------------------------------------------------------------------

-

D = 2031.0000

W = 0.9739

Critical W = 0.8840 (alpha = 0.01 , N = 24)

W = 0.9160 (alpha = 0.05 , N = 24)

---------------------------------------------------------------------------

-

Data PASS normality test (alpha = 0.01). Continu e analysis.

Title: NICOSULFURON LEMNA 14 DAY FROND NUMBER

File: 4308F Transform: NO

TRANSFORMATION

Levene's Test for Homogeneity of Variance

ANOVA Table

---------------------------------------------------------------------------

-

SOURCE DF SS MS F

---------------------------------------------------------------------------

-

Bet ween 5 255.5000 51.1000 1.3687

Within (Error) 18 672.0000 37.3333

---------------------------------------------------------------------------

-

Total 23 927.5 000

---------------------------------------------------------------------------

-

(p

- value = 0.2821)

Critical F = 4.2479 (alpha = 0.01, df = 5,18)

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) 75WG and

Nicosulfuron 75WG plus Isodecylalcohol Ethoxylated (IN

- KG691) Surfactant to Aquatic

Vascular Plants, Lemna gibba G3

PMRA Submission Number 

### {........} EPA MRID Number 48354308

Page 22 of 46 = 2.7729 ( alpha = 0.05, df = 5,18)

Since F < Critical F FAIL TO REJECT Ho: All equal (alpha = 0.01)

Title: NICOSULFURON LEMNA 14 DAY FROND NUMBER

File: 4308F Transform: NO

TRANSFORMATION

ANOVA Table

---------------------------------------------------------------------------

-

SOURCE DF SS MS F

----------------------------------------------------------------- ----------

-

Between 5 858335.0000 171667.0000 1521.4210

Within (Error) 18 2031.0000 112.8333

---------------------------------------------------------------------------

-

Total 23 860366.0000

---------------------------------------------------------------------------

-

(p

- value = 0.0000)

Critical F = 4.2479 (alpha = 0.01, df = 5,18)

= 2. 7729 (alpha = 0.05, df = 5,18)

Since F > Critical F REJECT Ho: All equal (alpha = 0.05)

Title: NICOSULFURON LEMNA 14 DAY FROND NUMBER

File: 4308F Transform: NO

TRANSFORMATION

William's Test

- TABLE 1 OF 2 Ho: Control<Treatment

---------------------------------------------------------------------------

-

ORIGINAL TRANSFORMED ISOTONIZED

GROUP IDENTIFICATION N MEAN MEAN MEAN

------ -------------------- --- ----------- ----------- ----------

-

1 NEG CONTROL 4 459.0000 459.0000 473.4375

2 0.424 4 480.7500 480.7500 473.4375

3 0.789 4 476.7500 476.7500 473.4375

4 1.690 4 477.2500 477.2500 473.4375

5 3.300 4 108.0000 108.0000 108.0000

6 6.400 4 41.2500 41.2500 41.2500

---------------------------------------------------------------------------

-

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) 75WG and

Nicosulfuron 75WG plus Isodecylalcohol Ethoxylated (IN

- KG691) Surfactant to Aquatic

Vascular Plants, Lemna gibba G3

PMRA Submission Number 

### {........} EPA MRID Number 48354308

Page 23 of 46 Title: NICOSULFURON LEMNA 14 DAY FROND NUMBER

File: 4308F Transform: NO

TRANSFORMATION

William's Test

- TABLE 2 OF 2 Ho: Control<Treatment

---------------------------------------------------------------------------

-

COMPARED CALC. SIG TABLE DEGREES OF

IDENTIFICATION MEANS WILLIAMS 0.05 WILLIAMS FREEDOM

USED

-------------------- ----------- ----------- ----- ----------- -----------

-

NEG CONTROL 459.0000

0.424 473.4375

- 1.9 222 1.7300 k= 1,

v=18

0.789 473.4375

- 1.9222 1.8200 k= 2,

v=18

1.690 473.4375

- 1.9222 1.8500 k= 3,

v=18

3.300 108.0000 46.7308 * 1.8600 k= 4,

v=18

6.400 41.2500 55.6177 * 1.8700 k= 5,

v=18

---------------------------------------------------------------------------

-

s = 10.6223

WARNING: Procedure has used isotonized means which differ from original

(transformed) means.

------------------------------------------------------------------------

Estimates of EC%

------------------------------------------------------------------------

Parameter Estimate 95% Bounds Std.Err. Lower Bound

Lower Upper /Estimate

EC5 1.1 0.77 1.5 0.070 0.72

EC10 1.3 0.99 1.8 0.060 0.75

EC25 1.8 1.5 2.3 0.045 0.80

EC50 2.6 2.3 3.1 0.031 0.86

Slope = 4.23 Std.Err. = 0.492

!!!Poor fit: p < 0.001 based on DF= 3.00 18.0

------------------------------------------------------------------------

4308F : NICOSULFURON LEMNA 14 DAY FROND NUMBER

------------------------------------------------------------------------

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) 75WG and

Nicosulfuron 75WG plus Isodecylalcohol Ethoxylated (IN

- KG691) Surfactant to Aquatic

Vascular Plants, Lemna gibba G3

PMRA Submission Number 

### {........} EPA MRID Number 48354308

Page 24 of 46 Observed vs. Predicted T reatment Group Means

------------------------------------------------------------------------

Dose #Reps. Obs. Pred. Obs. Pred. %Change

Mean Mean

- Pred. %Control

0.00 4.00 459. 489.

- 30.2 100. 0.00

0.424 4.00 481. 489.

- 8.31 100. 0.0384

0.789 4.00 477. 483.

- 6.08 98.7 1.31

1.69 4.00 477. 389. 88.4 79.5 20.5

3.30 4.00 108. 167.

- 59.4 34.2 65.8

6.40 4.00 41.3 25.6 15.7 5.22 94.8

GROWTH RATE (0

- 7 DAYS)

Title: NICOSULFURON LEMNA 0

- 7 DAY GROWTH RATE

File: 4308g Transform: NO

TRANSFORMATION

Shapiro

- Wilk's Test for Normality

---------------------------------------------------------------------------

-

D = 0.0022

W = 0.9441

Critical W = 0.8840 (alpha = 0.01 , N = 24)

W = 0.9160 (alpha = 0.05 , N = 24)

---------------------------------------------------------------------------

-

Data PASS normality test (alpha = 0.01). Continu e analysis.

Title: NICOSULFURON LEMNA 0

- 7 DAY GROWTH RATE

File: 4308g Transform: NO

TRANSFORMATION

Levene's Test for Homogeneity of Variance

ANOVA Table

---------------------------------------------------------------------------

-

SOURCE DF SS MS F

---------------------------------------------------------------------------

-

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) 75WG and

Nicosulfuron 75WG plus Isodecylalcohol Ethoxylated (IN

- KG691) Surfactant to Aquatic

Vascular Plants, Lemna gibba G3

PMRA Submission Number 

### {........} EPA MRID Number 48354308

Page 25 of 46 Bet ween 5 0.0002 0.0000 1.1914

Within (Error) 18 0.0005 0.0000

---------------------------------------------------------------------------

-

Total 23 0.0 006

---------------------------------------------------------------------------

-

(p

- value = 0.3523)

Critical F = 4.2479 (alpha = 0.01, df = 5,18)

= 2.7729 ( alpha = 0.05, df = 5,18)

Since F < Critical F FAIL TO REJECT Ho: All equal (alpha = 0.01)

Title: NICOSULFURON LEMNA 0

- 7 DAY GROWTH RATE

File: 4308g Transform: NO

TRANSFORMATION

ANOVA Table

---------------------------------------------------------------------------

-

SOURCE DF SS MS F

------------------------------------------------------------ ---------------

-

Between 5 0.2087 0.0417 334.8018

Within (Error) 18 0.0022 0.0001

---------------------------------------------------------------------------

-

Total 23 0.2109

---------------------------------------------------------------------------

-

(p

- value = 0.0000)

Critical F = 4.2479 (alpha = 0.01, df = 5,18)

= 2.7729 (alpha = 0.05, df = 5,18)

Since F > Critical F REJECT Ho: All equal (alpha = 0.05)

Title: NICOSULFURON LEMNA 0

- 7 DAY GROWTH RATE

File: 4308g Transform: NO

TRANSFORMATION

William's Test

- TABLE 1 OF 2 Ho: Control<Treatment

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) 75WG and

Nicosulfuron 75WG plus Isodecylalcohol Ethoxylated (IN

- KG691) Surfactant to Aquatic

Vascular Plants, Lemna gibba G3

PMRA Submission Number 

### {........} EPA MRID Number 48354308

Page 26 of 46 ---------------------------------------------------------------------------

-

ORIGINAL TRANSFORMED ISOTONIZED

GROUP IDENTIFICATION N MEAN MEAN MEAN

------ -------------------- --- ----------- ----------- ----------

-

1 NEG CONTROL 4 0.3235 0.3235 0.3308

2 0.424 4 0.3347 0.3347 0.3308

3 0.789 4 0.3340 0.3340 0.3308

4 1.690 4 0.2878 0.2878 0.2878

5 3.300 4 0.1343 0.1343 0.1343

6 6.400 4 0.1163 0.1163 0.1163

---------------------------------------------------------------------------

-

Title: NICOSULFURON LEMNA 0

- 7 DAY GROWTH RATE

File: 4308g Transform: NO

TRANSFORMATION

William's Test

- TABLE 2 OF 2 Ho: Control<Treatment

---------------------------------------------------------------------------

-

COMPARED CALC. SIG TABLE DEGREES OF

IDENTIFICATION MEANS WILLIAMS 0.05 WILLIAMS FREEDOM

USED

-------------------- ----------- ----------- ----- ----------- -----------

-

NEG CONTROL 0.3235

0.424 0.3308

- 0.9152 1.7300 k= 1,

v=18

0.789 0.3308

- 0.9152 1.8200 k= 2,

v=18

1.690 0.2878 4.5252 * 1.8500 k= 3,

v=18

3. 300 0.1343 23.9685 * 1.8600 k= 4,

v=18

6.400 0.1163 26.2454 * 1.8700 k= 5,

v=18

---------------------------------------------------------------------------

-

s = 0.0112

WARNING: Procedure has used isotonized means which differ from original

(transformed) means.

------------------------------------------------------------------------

Estimates of EC%

--------------------- ---------------------------------------------------

Parameter Estimate 95% Bounds Std.Err. Lower Bound

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) 75WG and

Nicosulfuron 75WG plus Isodecylalcohol Ethoxylated (IN

- KG691) Surfactant to Aquatic

Vascular Plants, Lemna gibba G3

PMRA Submission Number 

### {........} EPA MRID Number 48354308

Page 27 of 46 Lower Upper /Estimate

EC5 0.60 0.31 1.2 0.14 0.51

EC10 0.89 0.51 1.6 0.12 0.57

EC25 1.7 1.2 2.5 0.079 0.69

EC50 3.5 2.9 4.4 0.044 0.81

Slope = 2.14 Std.Err. = 0.311

!!!Poor fit: p < 0.001 based on DF= 3.00 18.0

------------------------------------------------------------------------

4308G : NICOSULFURON LEMNA 0

- 7 DAY GROWTH RATE

------------------------------------------------------------------------ Observed vs. Predicted Treatment Group Means

------------------------------------------------------------------------

Dose #Reps. Obs. Pred. Obs. Pred. %Change

Mean Mean

- Pred. %Control

0.00 4.00 0.324 0.343

- 0.0192 100. 0.00

0.424 4.00 0.335 0.334 0.000332 97.6 2.42

0.789 4.00 0.334 0.315 0.0192 91.9 8.13

1.69 4.00 0.288 0.259 0.0293 75.4 24.6

3.30 4.00 0.134 0.180

- 0.0461 52.6 47.4

6.40 4.00 0.116 0.0998 0.0165 29.1 70.9

GROWTH RATE (0

- 14 DAYS)

Title: NICOSULFURON LEMNA 0

- 14 DA Y GROWTH RATE

File: 4308lg Transform: NO

TRANSFORMATION

Shapiro

- Wilk's Test for Normality

---------------------------------------------------------------------------

-

D = 0.0002

W = 0.9039

Critical W = 0.8840 (alpha = 0.01 , N = 24)

W = 0.9160 (alpha = 0.05 , N = 24)

---------------------------------------------------------------------------

-

Data PASS normality test (al pha = 0.01). Continue analysis.

Title: NICOSULFURON LEMNA 0

- 14 DAY GROWTH RATE

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) 75WG and

Nicosulfuron 75WG plus Isodecylalcohol Ethoxylated (IN

- KG691) Surfactant to Aquatic

Vascular Plants, Lemna gibba G3

PMRA Submission Number 

### {........} EPA MRID Number 48354308

Page 28 of 46 File: 4308lg Transform: NO

TRANSFORMATION

Levene's Test for Homogeneity of Variance

ANOVA Table

---------------------------------------------------------------------------

-

SOURCE DF SS MS F

----------------------------------------------------------------- ----------

-

Between 5 0.0000 0.0000 1.6378

Within (Error) 18 0.0001 0.0000

---------------------------------------------------------------------------

-

Total 23 0.0001

---------------------------------------------------------------------------

-

(p

- value = 0.2008)

Critical F = 4.2479 (alpha = 0.01, df = 5,18)

= 2.7729 (alpha = 0.05, df = 5,18)

Since F < Critical F FAIL TO REJECT Ho: All equal (alpha = 0.01)

Title: NICOSULFURON LEMNA 0

- 14 DAY GROWTH RATE

File: 4308lg Transform: NO

TRANSFORMATION

Shapiro

- Wilk's Test for Normality

---------------------------------------------------------------------------

-

D = 0.0002

W = 0.9039

Critical W = 0.8840 (alpha = 0.01 , N = 24)

W = 0.9160 (alpha = 0.05 , N = 24)

---------------------------------------------------------------------------

-

Data PASS normality test (alpha = 0.01). Continue analysis.

Title: NICOSULFURON LEMNA 0

- 14 DAY GROWTH RATE

File: 4308lg Transform: NO

TRANSFORMATION

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) 75WG and

Nicosulfuron 75WG plus Isodecylalcohol Ethoxylated (IN

- KG691) Surfactant to Aquatic

Vascular Plants, Lemna gibba G3

PMRA Submission Number 

### {........} EPA MRID Number 48354308

Page 29 of 46 Levene's Test for Homogeneity of Variance

ANOVA Table

---------------------------------------------------------------------------

-

SOURCE DF SS MS F

---------------------------------------------------------------------------

-

Between 5 0.0000 0.0000 1.6378

Within (Error) 1 8 0.0001 0.0000

---------------------------------------------------------------------------

-

Total 23 0.0001

---------------------------------------------------------------------------

-

(p

- value = 0.2008)

Critical F = 4.2479 (alpha = 0.01, df = 5,18)

= 2.7729 (alpha = 0.05, df = 5,18)

Since F < Critical F FAIL TO REJECT Ho: All equal (alpha = 0.01)

------ ------------------------------------------------------------------

Estimates of EC%

------------------------------------------------------------------------

Parameter Estimate 95% Bounds Std.Err. Lower Bound

Lower Upper /Estimate

EC5 1.3 1.0 1.6 0.048 0.79

EC10 1.6 1.4 2.0 0.040 0.83

EC25 2.5 2.2 2.9 0.02 7 0.88

EC50 4.1 3.8 4.5 0.016 0.93

Slope = 3.21 Std.Err. = 0.239

!!!Poor fit: p < 0.001 based on DF= 3.00 18.0

------------------------------------------------------------------------

4308LG : NICOSULFURON LEMNA 0

- 14 DAY GROWTH RATE

------------------------------------------------------------------------ Observed vs. Predicted Treatment Group Means

------------------------------------------------------------- -----------

Dose #Reps. Obs. Pred. Obs. Pred. %Change

Mean Mean

- Pred. %Control

0.00 4.00 0.244 0.251

- 0.00665 100. 0.00

0.424 4.00 0.248 0.251

- 0.00316 99.9 0.0748

0.789 4.00 0.247 0.248

- 0.00133 99.0 1.05

1.69 4.00 0.247 0.224 0.0228 89.4 10.6

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) 75WG and

Nicosulfuron 75WG plus Isodecylalcohol Ethoxylated (IN

- KG691) Surfactant to Aquatic

Vascular Plants, Lemna gibba G3

PMRA Submission Number 

### {........} EPA MRID Number 48354308

Page 30 of 46 3.30 4.00 0.141 0.156

- 0.0155 62.3 37.7

6.40 4.00 0.0720 0.0681 0.00390 27.1 72.9

BIOMASS

Title: NICOSULFURON LEMNA 14 DAY BIOMASS

File: 4308b Transform: NO

TRANSFORMATION

Shapiro

- Wilk's Test for Normality

---------------------------------------------------------------------------

-

D = 52.0200

W = 0.9486

Critical W = 0.8580 (alpha = 0.01 , N = 18)

W = 0.8970 (alpha = 0.05 , N = 18)

-------- -------------------------------------------------------------------

-

Data PASS normality test (alpha = 0.01). Continue analysis.

Title: NICOSULFURON LEMNA 14 DAY BIOMASS

File: 4308b Transform: NO

TRANSFORMATION

Levene's Test for Homogeneity of Variance

ANOVA Table

---------------------------------------------------------------------------

-

SOURCE DF SS MS F

---------------------------------------------------------------------------

-

Between 5 7.2361 1.4472 0.6163

Within (Error) 1 2 28.1800 2.3483

---------------------------------------------------------------------------

-

Total 17 35.4161

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) 75WG and

Nicosulfuron 75WG plus Isodecylalcohol Ethoxylated (IN

- KG691) Surfactant to Aquatic

Vascular Plants, Lemna gibba G3

PMRA Submission Number 

### {........} EPA MRID Number 48354308

Page 31 of 46 ---------------------------------------------------------------------------

-

(p

- value = 0.6901)

Critical F = 5.0643 (alpha = 0.01, df = 5,12)

= 3.1059 (alpha = 0.05, df = 5,12)

Since F < Critical F FAIL TO REJECT Ho: All equal (alpha = 0.01)

Title: NICOSULFURON LEMNA 14 DAY BIOMASS

File: 4308b Transform: NO

TRANSFORMATION

ANOVA Table

------------------------------------------------------------------ ---------

-

SOURCE DF SS MS F

---------------------------------------------------------------------------

-

Between 5 3119.6294 623.9259 143.9275

Within (Error) 12 52.0200 4.3350

---------------------------------------------------------------------------

-

Total 17 3171.6494

---------------------------------------------------------------- -----------

-

(p

- value = 0.0000)

Critical F = 5.0643 (alpha = 0.01, df = 5,12)

= 3.1059 (alpha = 0.05, df = 5,12)

Since F > Critical F REJECT Ho: All equal (alpha = 0.05)

Title: NICOSULFURON LEMNA 14 DAY BIOMASS

File: 4308b Transform: NO

TRANSFORMATION

William's Test

- TABLE 1 OF 2 Ho: Control<Treatment

------------------------ ---------------------------------------------------

-

ORIGINAL TRANSFORMED ISOTONIZED

GROUP IDENTIFICATION N MEAN MEAN MEAN

------ -------------------- --- ---------- - ----------- ----------

-

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) 75WG and

Nicosulfuron 75WG plus Isodecylalcohol Ethoxylated (IN

- KG691) Surfactant to Aquatic

Vascular Plants, Lemna gibba G3

PMRA Submission Number 

### {........} EPA MRID Number 48354308

Page 32 of 46 1 NEG CONTROL 3 43.7333 43.7333 44.3333

2 0.424 3 44.9333 44.9333 44.3333

3 0.789 3 43.6667 43.6667 4 3.6667

4 1.690 3 37.9333 37.9333 37.9333

5 3.300 3 16.3333 16.3333 16.3333

6 6.400 3 13.8333 13.8333 13.8333

---------------------------------------------------------------------------

-

Title: NICOSULFURON LEMNA 14 DAY BIOMASS

File: 4308b Transform: NO

TRANSFORMATION

William's Test

- TABLE 2 OF 2 Ho: Control<Treatment

---------------------------------------------------------------------------

-

COMPARED CALC. SIG TABLE DEGREES OF

IDENTIFICATION MEANS WILLIAMS 0.05 WILLIAMS FREEDOM

USED

-------------------- ----------- ----------- ----- ----------- -----------

-

NEG CONTROL 43.7333

0.424 44.3333

- 0.3529 1.7800 k= 1,

v=12

0.789 43.6667 0. 0392 1.8700 k= 2,

v=12

1.690 37.9333 3.4118 * 1.9000 k= 3,

v=12

3.300 16.3333 16.1176 * 1.9200 k= 4,

v=12

6.400 13.8333 17.5882 * 1.9 300 k= 5,

v=12

---------------------------------------------------------------------------

-

s = 2.0821

WARNING: Procedure has used isotonized means which differ from original

(transformed) means.

--------------------------- ---------------------------------------------

Estimates of EC%

------------------------------------------------------------------------

Parameter Estimate 95% Bounds Std.Err. Lower Bound

Lower Upper /Estimate

EC5 0.58 0.27 1.3 0.16 0.46

EC10 0.84 0.44 1.6 0.13 0.52

EC25 1.6 1.0 2.5 0.092 0.64

EC50 3.2 2.5 4.2 0.053 0.77

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) 75WG and

Nicosulfuron 75WG plus Isodecylalcohol Ethoxylated (IN

- KG691) Surfactant to Aquatic

Vascular Plants, Lemna gibba G3

PMRA Submission Number 

### {........} EPA MRID Number 48354308

Page 33 of 46 Slope = 2.21 Std.Err. = 0.360

!!!Poor fit: p < 0.001 based on DF= 3.00 12.0

------------------------------------------------------------------------

4308B : NICOSULFURON LEMN A 14 DAY BIOMASS

------------------------------------------------------------------------ Observed vs. Predicted Treatment Group Means

------------------------------------------------------------------------

Dose #Reps. Obs. Pred. Obs. Pred. %Change

Mean Mean

- Pred. %Control

0.00 3.00 43.7 46.0

- 2.25 100. 0.00

0.424 3.00 44.9 44.8 0.148 97.4 2.60

0.789 3.00 43.7 41.9 1.78 91.1 8.90

1.69 3.00 37.9 33.6 4.32 73.1 26.9

3.30 3.00 16.3 22.5

- 6.16 48.9 51.1

6.40 3.00 13.8 11.7 2.17 25.4 74.6

NICOSULFURON 75WG PLUS IN

- KG691 SURFACTANT STUDY

FROND NUMBER

Title: NICOSULFURON+IN

- KG691 LEMNA 14 DAY FROND NUMBER

File: 4308sf Transform: NO

TRANSFORMATION

Shapiro

- Wilk's Test for Normality

---------------------------------------------------------------------------

-

D = 1978.0000

W = 0.9582

Critical W = 0.8840 (alpha = 0.01 , N = 24)

W = 0.916 0 (alpha = 0.05 , N = 24)

---------------------------------------------------------------------------

-

Data PASS normality test (alpha = 0.01). Continue analysis.

Title: NICOSULFURON+IN

- KG691 LEMNA 14 DAY FROND NUMBER

File: 4308sf Transform: NO

TRANSFORMATION

Levene's Test for Homogeneity of Variance

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) 75WG and

Nicosulfuron 75WG plus Isodecylalcohol Ethoxylated (IN

- KG691) Surfactant to Aquatic

Vascular Plants, Lemna gibba G3

PMRA Submission Number 

### {........} EPA MRID Number 48354308

Page 34 of 46 ANOVA Table

------------------------------------------------------------------- --------

-

SOURCE DF SS MS F

---------------------------------------------------------------------------

-

Between 5 133.5000 26.7000 0.6975

Within (E rror) 18 689.0000 38.2778

---------------------------------------------------------------------------

-

Total 23 822.5000

---------------------------------------------------------------------------

-

(p

- value = 0.6322)

Critical F = 4.2479 (alpha = 0.01, df = 5,18)

= 2.7729 (alpha = 0.05, df = 5,18)

Since F < Critical F FAIL TO REJECT Ho: All equal (alpha = 0.01 )

Title: NICOSULFURON+IN

- KG691 LEMNA 14 DAY FROND NUMBER

File: 4308sf Transform: NO

TRANSFORMATION

ANOVA Table

---------------------------------------------------------------------------

-

SOURCE DF SS MS F

---------------------------------------------------------------------------

-

Between 5 947172.5000 189434.5000 1723.8731

Within (Error) 18 1978.0000 109.8889

---------------------------------------------------------------------------

-

Total 23 949150.5000

--------- ------------------------------------------------------------------

-

(p

- value = 0.0000)

Critical F = 4.2479 (alpha = 0.01, df = 5,18)

= 2.7729 (alpha = 0.05, df = 5,18)

Since F > Critical F REJECT Ho: All equal (alpha = 0.05)

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) 75WG and

Nicosulfuron 75WG plus Isodecylalcohol Ethoxylated (IN

- KG691) Surfactant to Aquatic

Vascular Plants, Lemna gibba G3

PMRA Submission Number 

### {........} EPA MRID Number 48354308

Page 35 of 46 Title: NICOSULFURON+IN

- KG691 LEMNA 14 DAY FROND NUMBER

File: 4308sf Transform: NO

TRANSFORMATION

William's Test

- TABLE 1 OF 2 Ho: Control<Treatment

---------------------------------------------------------------------------

-

ORIGINAL TRANSFORMED ISOTONIZED

GROUP IDENTIFICATION N MEAN MEAN MEAN

------ -------------------- --- ----------- ----------- ----------

-

1 NEG CONTROL 4 489.2500 489.2500 490.0000

2 0.389 4 490.7500 490.7500 490.0000

3 0.737 4 478.0000 478.0000 478.0000

4 1.520 4 448.5000 448.5000 448.5000

5 5.225 4 77.7500 77.7500 77.7500

6 8 .135 4 36.2500 36.2500 36.2500

---------------------------------------------------------------------------

-

Title: NICOSULFURON+IN

- KG691 LEMNA 14 DAY FROND NUMBER

File: 4308sf Transform: NO

TRANSFORMATION

William's Test

- TABLE 2 OF 2 Ho: Control<Treatment

---------------------------------------------------------------------------

-

COMPARED CALC. SIG TABLE DEGREES OF

IDENTIFICATION MEANS WILLIAMS 0.05 WILLIAMS FREEDOM

USED

-------------------- ----------- ----------- ----- ----------- -----------

-

NEG CONTROL 489.2500

0.389 490.0000

- 0.1012 1.7300 k= 1,

v=18

0.737 478.0000 1.5177 1.8200 k= 2,

v=18

1.520 448.5000 5.4975 * 1.8500 k= 3,

v=18

5.225 77.7500 55.5147 * 1.8600 k= 4,

v=18

8.135 36.2500 61.1134 * 1.8700 k= 5,

v=18

---------------------------------------------------------------------------

-

s = 10.4828

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) 75WG and

Nicosulfuron 75WG plus Isodecylalcohol Ethoxylated (IN

- KG691) Surfactant to Aquatic

Vascular Plants, Lemna gibba G3

PMRA Submission Number 

### {........} EPA MRID Number 48354308

Page 36 of 46 WARNING: Procedure has used isotonized means which differ from original

( transformed) means.

------------------------------------------------------------------------

Estimates of EC%

------------------------------------------------------------------------

Parameter Estimate 95% Bounds Std.Err. Lower Bound

Lower Upper /Estimate

EC5 1.1 0.85 1.3 0.045 0.81

EC10 1.3 1.1 1.6 0.040 0.83

EC25 2.0 1.7 2.3 0.030 0.86

EC50 3.0 2.7 3.3 0.021 0.91

Slope = 3.64 Std.Err. = 0.213

!!!Poor fit: p < 0.001 based on DF= 3.00 18.0

---------------------------------- --------------------------------------

4308SF : NICOSULFURON+IN

- KG691 LEMNA 14 DAY FROND NUMBER

------------------------------------------------------------------------ Observed vs. Predicted Treatment Group Means

-------------- ----------------------------------------------------------

Dose #Reps. Obs. Pred. Obs. Pred. %Change

Mean Mean

- Pred. %Control

0.00 4.00 489. 494.

- 4.75 100. 0.00

0.389 4.00 491. 494.

- 2.94 99.9 0.0628

0.737 4.00 478. 487.

- 9.41 98.7 1.34

1.52 4.00 449. 424. 24.7 85.8 14.2

5.22 4.00 77.8 93.5

- 15.7 18.9 81.1

8.13 4.00 36.3 28.1 8.11 5.70 94.3

GROWTH RATE (0

- 7 DAYS)

Title: NICOSULFURON+IN

- KG691 LEMNA 0

- 7 DAY GROWTH RATE

File: 4308s g Transform: NO

TRANSFORMATION

Shapiro

- Wilk's Test for Normality

---------------------------------------------------------------------------

-

D = 0.0032

W = 0.9689

Critical W = 0. 8840 (alpha = 0.01 , N = 24)

W = 0.9160 (alpha = 0.05 , N = 24)

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) 75WG and

Nicosulfuron 75WG plus Isodecylalcohol Ethoxylated (IN

- KG691) Surfactant to Aquatic

Vascular Plants, Lemna gibba G3

PMRA Submission Number 

### {........} EPA MRID Number 48354308

Page 37 of 46 ---------------------------------------------------------------------------

-

Data PASS normality test (alpha = 0.01). Continue analysis.

Title: NICOSULFURON+IN

- KG691 LEMNA 0

- 7 DAY GROWTH RATE

File: 4308sg Transform: NO

TRANSFORMATION

Levene's Test for Homogeneity of Variance

ANOVA Table

- --------------------------------------------------------------------------

-

SOURCE DF SS MS F

---------------------------------------------------------------------------

-

Between 5 0.0001 0.0000 0.1527

Within (Error) 18 0.0015 0.0001

---------------------------------------------------------------------------

-

Total 23 0.0015

------------ ---------------------------------------------------------------

-

(p

- value = 0.9765)

Critical F = 4.2479 (alpha = 0.01, df = 5,18)

= 2.7729 (alpha = 0.05, df = 5,18)

Since F < Critical F FAIL TO REJECT Ho: All equal (alpha = 0.01)

Title: NICOSULFURON+IN

- KG691 LEMNA 0

- 7 DAY GROWTH RATE

File: 4308sg Transform: NO

TRANSFORMATION

ANOVA Table

---------------------------------------------------------------------------

-

SOURCE DF SS MS F

-------------------------------------------------------- -------------------

-

Between 5 0.2277 0.0455 254.6673

Within (Error) 18 0.0032 0.0002

---------------------------------------------------------------------------

-

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) 75WG and

Nicosulfuron 75WG plus Isodecylalcohol Ethoxylated (IN

- KG691) Surfactant to Aquatic

Vascular Plants, Lemna gibba G3

PMRA Submission Number 

### {........} EPA MRID Number 48354308

Page 38 of 46 Total 23 0.2309

---------------------------------------------------------------------------

-

(p

- value = 0.0000)

Critical F = 4.2479 (alpha = 0.01, df = 5,18)

= 2.7729 (alpha = 0.05, df = 5,18)

Since F > Critical F REJECT Ho: All equal (alpha = 0.05)

Title: NICOSULFURON+IN

- KG691 LEMNA 0

- 7 DAY GROWTH RATE

File: 4308sg Transform: NO

TRANSFORMATIO N

William's Test

- TABLE 1 OF 2 Ho: Control<Treatment

---------------------------------------------------------------------------

-

ORIGINAL TRANSFORMED ISOTONIZED

GROUP IDENTIFICATION N MEAN MEAN MEAN

------ -------------------- --- ----------- ----------- ----------

-

1 NEG CONTROL 4 0.3538 0.3538 0.3538

2 0.389 4 0.3493 0.3493 0.3510

3 0.737 4 0.3527 0.3527 0.3510

4 1.520 4 0.3197 0.3197 0.3197

5 5.225 4 0.1563 0.1563 0.1563

6 8.135 4 0.1231 0.1231 0.1231

---------------------------------------------------------------------------

-

Title: NICOSULFURON+IN

- KG691 LEMNA 0

- 7 DAY GROWTH RATE

File: 4308sg Transform: NO

TRANSFORMATION

William's Test

- TABLE 2 OF 2 Ho: Control<Treatment

---------------------------------------------------------------------------

-

COM PARED CALC. SIG TABLE DEGREES OF

IDENTIFICATION MEANS WILLIAMS 0.05 WILLIAMS FREEDOM

USED

-------------------- ----------- ----------- ----- ----------- -----------

-

NEG CONTROL 0.3538

0.389 0.3510 0.2922 1.7300 k= 1,

v=18

0.737 0.3510 0.2922 1.8200 k= 2,

v=18

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) 75WG and

Nicosulfuron 75WG plus Isodecylalcohol Ethoxylated (IN

- KG691) Surfactant to Aquatic

Vascular Plants, Lemna gibba G3

PMRA Submission Number 

### {........} EPA MRID Number 48354308

Page 39 of 46 1.520 0.3197 3.5984 * 1.8500 k= 3,

v=18

5.225 0.156 3 20.8819 * 1.8600 k= 4,

v=18

8.135 0.1231 24.3957 * 1.8700 k= 5,

v=18

---------------------------------------------------------------------------

-

s = 0.0134

WARNING: Procedure has used isotonized means which differ from original

(transformed) means.

------------------------------------------------------------------------

Estimates of EC%

------------------------------ ------------------------------------------

Parameter Estimate 95% Bounds Std.Err. Lower Bound

Lower Upper /Estimate

EC5 0.84 0.54 1.3 0.093 0.64

EC10 1.2 0.86 1.8 0.077 0.69

EC25 2.4 1.9 3.0 0.051 0.78

EC50 4.9 4.4 5.6 0.025 0.89

Slope = 2.14 Std.Err. = 0.210

!!!Poor fit: p = 0.040 based on DF= 3.0 18.

------------------------------------------------------------------------

4308SG : NICOSULFURON+IN

- KG691 LEMNA 0

- 7 DAY GROWTH RATE

------------------------------------------------------------------------

Observed vs. Predicted Treatment Grou p Means

------------------------------------------------------------------------

Dose #Reps. Obs. Pred. Obs. Pred. %Change

Mean Mean

- Pred. %Control

0.00 4.00 0.354 0.359

- 0.00507 100. 0.00

0.389 4.00 0.349 0.356

- 0.00630 99.1 0.912

0.737 4.00 0.353 0.345 0.00779 96.1 3.87

1.52 4.00 0.320 0.310 0.0102 86.3 13.7

5.22 4.00 0.156 0.171

- 0.0150 47.7 52.3

8.13 4.00 0.123 0.115 0.00839 32.0 68.0

GROWTH RATE (0

- 14 DAYS)

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) 75WG and

Nicosulfuron 75WG plus Isodecylalcohol Ethoxylated (IN

- KG691) Surfactant to Aquatic

Vascular Plants, Lemna gibba G3

PMRA Submission Number 

### {........} EPA MRID Number 48354308

Page 40 of 46 Titl e: NICOSULFURON+IN

- KG691 LEMNA 0

- 14 DAY GROWTH RATE

File: 4308slg Transform: OPTIMAL

POWER

Shapiro

- Wilk's Test for Normality

---------------------------------------------------------- -----------------

-

D = 0.0000

W = 0.9801

Critical W = 0.8840 (alpha = 0.01 , N = 24)

W = 0.9160 (alpha = 0.05 , N = 24)

---------------------------------------------------------------------------

-

Data PASS normality test (alpha = 0.01). Continue analysis.

Title: NICOSULFURON+IN

- KG691 LEMNA 0

- 14 DAY GROWTH RATE

File: 4308slg Transform: OPTIMAL

POWER

Levene's Test for Homogeneity of Variance

ANOVA Table

---------------------------------------------------------------------------

-

SOURCE DF SS MS F

------------------------------------------ ---------------------------------

-

Between 5 0.0000 0.0000 0.2986

Within (Error) 18 0.0000 0.0000

---------------------------------------------------------------------------

-

Total 23 0.0000

---------------------------------------------------------------------------

-

(p

- value = 0.9073)

Critical F = 4.2479 (alpha = 0.01, df = 5,18)

= 2.7729 (alpha = 0.05, df = 5,18)

Since F < Critical F FAIL TO REJECT Ho: All equal (alpha = 0.01)

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) 75WG and

Nicosulfuron 75WG plus Isodecylalcohol Ethoxylated (IN

- KG691) Surfactant to Aquatic

Vascular Plants, Lemna gibba G3

PMRA Submission Number 

### {........} EPA MRID Number 48354308

Page 41 of 46 Title: NICOSULFURON+IN

- KG691 LEMNA 0

- 14 DAY GROWTH RATE

File: 4308slg Transform : OPTIMAL

POWER

ANOVA Table

---------------------------------------------------------------------------

-

SOURCE DF SS MS F

----------------------- ----------------------------------------------------

-

Between 5 0.0151 0.0030 1941.0058

Within (Error) 18 0.0000 0.0000

---------------------------------------------------------- -----------------

-

Total 23 0.0151

---------------------------------------------------------------------------

-

(p

- value = 0.0000)

Critical F = 4.2479 (alph a = 0.01, df = 5,18)

= 2.7729 (alpha = 0.05, df = 5,18)

Since F > Critical F REJECT Ho: All equal (alpha = 0.05)

Title: NICOSULFURON+IN

- KG691 LEMNA 0

- 14 DAY GROWTH RATE

File: 4308slg Transform: OPTIMAL

POWER

William's Test

- TABLE 1 OF 2 Ho: Control<Treatment

---------------------------------------------------------------------------

-

ORIGINAL TRANSFORMED ISOTONIZED

GROUP IDENTIFICATION N MEAN MEAN MEAN

------ -------------------- --- ----------- ----------- ----------

-

1 NEG CONTROL 4 0.2489 0.0628 0.0629

2 0.389 4 0.2491 0.0629 0.0629

3 0.737 4 0.2472 0.0620 0.0620

4 1.520 4 0.2427 0.0597 0.0 597

5 5.225 4 0.1173 0.0141 0.0141

6 8.135 4 0.0616 0.0041 0.0041

---------------------------------------------------------------------------

-

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) 75WG and

Nicosulfuron 75WG plus Isodecylalcohol Ethoxylated (IN

- KG691) Surfactant to Aquatic

Vascular Plants, Lemna gibba G3

PMRA Submission Number 

### {........} EPA MRID Number 48354308

Page 42 of 46 Title: NIC OSULFURON+IN

- KG691 LEMNA 0

- 14 DAY GROWTH RATE

File: 4308slg Transform: OPTIMAL

POWER

William's Test

- TABLE 2 OF 2 Ho: Control<Treatment

--------------------------------------------------- ------------------------

-

COMPARED CALC. SIG TABLE DEGREES OF

IDENTIFICATION MEANS WILLIAMS 0.05 WILLIAMS FREEDOM

USED

-------------------- ----------- ----------- ----- ----------- ------- ----

-

NEG CONTROL 0.0628

0.389 0.0629

- 0.0649 1.7300 k= 1,

v=18

0.737 0.0620 0.9500 1.8200 k= 2,

v=18

1.520 0.0597 3.4880 * 1.8500 k= 3,

v=18

5.225 0.0141 55.2985 * 1.8600 k= 4,

v=18

8.135 0.0041 66.6070 * 1.8700 k= 5,

v=18

----------------- ----------------------------------------------------------

-

s = 0.0012

WARNING: Procedure has used isotonized means which differ from original

(transformed) means.

----------------------------------------------------------------------- -

Estimates of EC%

------------------------------------------------------------------------

Parameter Estimate 95% Bounds Std.Err. Lower Bound

Lower Upper /Estimate

EC5 1.6 1.2 2.1 0.058 0.76

EC10 2.1 1.6 2.6 0.048 0.79

EC25 3.1 2.7 3.7 0.032 0.86

EC50 5.0 4.6 5.4 0.016 0.92

Slope = 3.30 Std.Err. = 0.295

Goodness of fit: p = 0.92 based on DF= 3.0 18.

------------------------------------------------------------------------

4308SLG : NICOSULFURON+IN

- KG691 LEMNA 0

- 14 DAY GROWTH RATE

------------------------------------------------------------------------ Observed vs. Predicted Treatment Group Means

------------------------------------------------------------------------

Dose #Reps. Obs. Pred. Obs. Pred. %Change

Mean Mean

- Pred. %Control

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) 75WG and

Nicosulfuron 75WG plus Isodecylalcohol Ethoxylated (IN

- KG691) Surfactant to Aquatic

Vascular Plants, Lemna gibba G3

PMRA Submission Number 

### {........} EPA MRID Number 48354308

Page 43 of 46 0.00 4.00 0.249 0.250

- 0.000792 100. 0.00

0.389 4.00 0.249 0.250

- 0.000536 100. 0.0124

0.737 4 .00 0.247 0.249

- 0.00172 99.7 0.299

1.52 4.00 0.243 0.239 0.00389 95.6 4.36

5.22 4.00 0.117 0.119

- 0.00160 47.6 52.4

8.13 4.00 0.0616 0.0608 0.000759 24.4 75.6

BIOMASS

Title: NICOSULFURON+IN

- KG691 LEMNA 14 DAY BIOMASS

File: 4308sb Transform: NO

TRANSFORMATION

Shapiro

- Wilk's Test for Normality

---------------------------------------------------------------------------

-

D = 55.0933

W = 0.9552

Critical W = 0.8580 (alpha = 0.01 , N = 18)

W = 0.8970 (alpha = 0.05 , N = 18)

---------------------------- -----------------------------------------------

-

Data PASS normality test (alpha = 0.01). Continue analysis.

Title: NICOSULFURON+IN

- KG691 LEMNA 14 DAY BIOMASS

File: 4308sb Transform: NO

TRANSFOR MATION

Levene's Test for Homogeneity of Variance

ANOVA Table

---------------------------------------------------------------------------

-

SOURCE DF SS MS F

---------------------------------------------------------------------------

-

Between 5 4.3667 0.8733 0.4010

Within (Error) 12 26.1333 2.1778

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) 75WG and

Nicosulfuron 75WG plus Isodecylalcohol Ethoxylated (IN

- KG691) Surfactant to Aquatic

Vascular Plants, Lemna gibba G3

PMRA Submission Number 

### {........} EPA MRID Number 48354308

Page 44 of 46 ---------------------------------------------------------------------------

-

Total 17 30.5000

---------------------------------------------------------------------------

-

(p

- value = 0.8390)

Critical F = 5.0643 (alpha = 0.01, df = 5,12)

= 3.1059 (alpha = 0.05, df = 5,12)

Since F < Critical F FAIL TO REJECT Ho: All equal (alpha = 0.01)

Title: NICOSULFURON+IN

- KG691 LEMNA 14 DAY BIOMASS

File: 4308sb Transform: NO

TRANSFORMATION

ANOVA Table

---------------------------------------------------------------------------

-

SOURCE DF SS MS F

---------------------------------------------------------------------------

-

Between 5 2532.0628 506.4126 110.3028

Within (Error) 12 55.0933 4.5911

---------------------------------------------------------------------------

-

Total 17 2587.1561

---------------------------------------------------------------------------

-

(p

- value = 0.0000)

Critical F = 5.0643 (alpha = 0.01, df = 5,12)

= 3.1059 (alpha = 0.05, df = 5,12)

Since F > Critical F REJECT Ho: All equal (alpha = 0.05)

Title: NICOSULFURON+IN

- KG691 LEMNA 14 DAY BIOMASS

File: 4308sb Transform: NO

TRANSFORMATION

William's Test

- TABLE 1 OF 2 Ho: Control<Treatment

---------------------------------------------------------------------------

-

ORIGINAL TRANSFORMED ISOTONIZED

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) 75WG and

Nicosulfuron 75WG plus Isodecylalcohol Ethoxylated (IN

- KG691) Surfactant to Aquatic

Vascular Plants, Lemna gibba G3

PMRA Submission Number 

### {........} EPA MRID Number 48354308

Page 45 of 46 GROUP IDENTIFICATION N MEAN MEAN MEAN

------ -------------------- --- ----------- ----------- ----------

-

1 N EG CONTROL 3 41.6333 41.6333 41.7333

2 0.389 3 41.8333 41.8333 41.7333

3 0.737 3 41.7000 41.7000 41.7000

4 1.520 3 36.066 7 36.0667 36.0667

5 5.225 3 17.3333 17.3333 17.3333

6 8.135 3 13.8667 13.8667 13.8667

-------------------------------------------------------------------- -------

-

Title: NICOSULFURON+IN

- KG691 LEMNA 14 DAY BIOMASS

File: 4308sb Transform: NO

TRANSFORMATION

William's Test

- TABLE 2 OF 2 Ho: Control<Treatment

---------------------------------------------------------------------------

-

COMPARED CALC. SIG TABLE DEGREES OF

IDENTIFICATION MEANS WILLIAMS 0.05 WILLIAMS FREEDOM

USED

------------------ -- ----------- ----------- ----- ----------- -----------

-

NEG CONTROL 41.6333

0.389 41.7333

- 0.0572 1.7800 k= 1,

v=12

0.737 41.7000

- 0.0381 1.8700 k= 2,

v=12

1.520 36.0667 3.1819 * 1.9000 k= 3,

v=12

5.225 17.3333 13.8897 * 1.9200 k= 4,

v=12

8.135 13.8667 15.8712 * 1.9300 k= 5,

v=12

---------------------- -----------------------------------------------------

-

s = 2.1427

WARNING: Procedure has used isotonized means which differ from original

(transformed) means.

------------------------------------------------------------------------

Est imates of EC%

------------------------------------------------------------------------

Parameter Estimate 95% Bounds Std.Err. Lower Bound

Lower Upper /Estimate

EC5 0.69 0.40 1.2 0.11 0.57

EC10 1.0 0.66 1.7 0.094 0.63

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) 75WG and

Nicosulfuron 75WG plus Isodecylalcohol Ethoxylated (IN

- KG691) Surfactant to Aquatic

Vascular Plants, Lemna gibba G3

PMRA Submission Number 

### {........} EPA MRID Number 48354308

Page 46 of 46 EC25 2.1 1.5 2.8 0.063 0.73

EC50 4.5 3.8 5.3 0.033 0. 85

Slope = 2.03 Std.Err. = 0.221

Goodness of fit: p = 0.12 based on DF= 3.0 12.

------------------------------------------------------------------------

4308SB : NICOSULFURON+IN

- KG691 LEMNA 14 DAY BIOMASS

------------------------------------------------------------------------ Observed vs. Predicted Treatment Group Means

------------------------------------------------------------------------

Dose #Reps. Obs. Pre d. Obs. Pred. %Change

Mean Mean

- Pred. %Control

0.00 3.00 41.6 42.7

- 1.08 100. 0.00

0.389 3.00 41.8 42.0

- 0.208 98.4 1.56

0.737 3.00 41.7 40.3 1.37 94.4 5.58

1.52 3.00 36.1 35.4 0.622 83.0 17.0

5.22 3.00 17.3 19.1

- 1.75 44.7 55.3

8.13 3.00 13.9 12.8 1.04 30.0 70.0