Test Type Report # Test System Comments Results

2-hr Approximate lethal concentration (ALC) DuPont 21871 Rat, whole- body Not GLP; Not Guideline The FEA-1100 male rat, whole-body inhalation 2-hr approxim

(ALC) was greater than 21,000 ppm. There were no deaths durrecovery period. 

4-hr LC50 DuPont 26154 RV1 Rat, nose-only GLP; Guideline The FEA-1100 male and female rat, nose-only inhalation 4-hr L

102,900 ppm. There were no deaths during the exposure or rec

Cardiac sensitization DuPont 25453 Dog GLP; Standard Protocol The FEA-1100 cardiac sensitization no-observed-adverse-effec

lowest-observed-adverse-effect level (LOAEL) in epinephrine 12,500 and 25,000 ppm, respectively

Dermal irritation DuPont 22199 Rabbit GLP; Guideline FEA-1100 was not a dermal irritant when tested in rabbits. No 

observed in the study.

Bacterial reverse mutation assay (Ames) DuPont 22597 Bacteria GLP; Guideline FEA-1100 was not mutagenic in the Bacterial Reverse Mutatio

using Salmonella strains TA98, TA100, TA1535, and TA1537 

uvr A with and without S9 activation.

In vitro chomosome aberration DuPont 22598 Human lymphocytes GLP; Guideline FEA-1100 did not produce structural or numerical chromosom

vitro mammalian chromosome aberration test using human perlymphocytes either in the presence or absence of S9-activated t

5-day inhalation range-finder DuPont 25309 Rat Not GLP; Not Guideline

Male and female rats were exposed to 0, 1000, 10,000 or 50,00nose-only inhalation for 6-hours per day for 5 consecutive daysgain, reduced food consumption and degeneration of the olfactneutrophilic infiltration and luminal cellular debris in the nasal the 10,000 and 50,000 ppm exposure levels. The no-observed-a(NOAEL) was 1000 ppm.

28-day inhalation DuPont 25850 Rat GLP; Guideline

Male and female rats were exposed to 0, 2500, 5000 or 10,000 

only inhalation for 6 hours per day, 5 days per week for 4 weekgain and reduced food consumption were observed at the 5000 exposure concentrations. The no-observed-adverse-effect leveppm.

90-day inhalation DuPont 17453-785 Rat GLP; Guideline

Male and female rats were exposed to 0, 500, 1500 or 10,000 pwhole-body inhalation for 6 hours per day, 5 days per week for body weight gain and reduced food consumption were observeexposure level. There were no adverse effects in the clinical or parameters in animals exposed to FEA-1100; therefore, the no-level (NOAEL) was 1500 ppm.

Pre-natal development DuPont 17453-731 Rat GLP; Guideline

Female rats were exposed to 500, 1500 or 10,000 ppm for 6 hogestation day (GD) 6-20. Reduced body weight gain, reduced reduced fetal body weight were observed at the 10,000 ppm exmalformations were observed at any exposure concentration. Teffect level (NOAEL) for maternal and fetal effects was 1500 p

Completed Studies CAS RN 692-49-9 - submitted with the PMN
90-day inhalation NA Rat GLP; Guideline

Male and female rats were exposed to 0, 3000, 4000, 5000 or 7whole-body inhalation for 6 hours per day, 5 days per week for verbal results, the no-observed-adverse-effect level (NOAEL) ippm for males and females, respectively. Male rats had significweight in the 7500 ppm exposure group, but no histopathologicin any test group. The study was initiated in August 2010 and tis August 2011.

In vivo micronucleus NA Rat GLP; Guideline

The micronucleus study was conducted as part of the on-going rats. Based on verbal results, the test substance was negative fovivo micronucleus test. The study was initiated in August 2010 data is August 2011. 

Ready biodegradability** A100560*** NA GLP; Guideline FEA-1100 is not readily biodegradable. No transformation pro

under the conditions of the test.

Partition coefficient** A100561*** NA GLP; Guideline The study was conducted using the HPLC Method for determin

partition coefficient (n-octanol/water). The partition coefficientest sustance was calculated to be 2.3.

Algae growth inhibition test** - - - In progressFish acute toxicity test** - - - In progressDaphnia acute immobilization test** - - - In progressExpected dates of final reports: 3Q2011

On-going Studies CAS RN 692-49-9

**Testing Facility: Environmental Risk Assessment CenterMitsubishi Chemical Medience Corporation1000 Kamoshida-cho, Aoba-ku, Yokohama, Kanagawa-ken 227-0033, JAPAN***Testing facility report number