# Patent

## Title
Pest control mixture

## Abstract

A pesticidal mixture including (a) spinetoram; and (b) a compound of group A selected from novaluron, pyriproxyfen, tau-fluvalinate, and a combination thereof.

## Background
This application is a national stage application of PCT/IL2013/050909, filed Nov. 5, 2013, which claims priority to U.S. Provisional Application Ser. No. 61/723,036, filed Nov. 6, 2012, which are hereby incorporated by reference in their entirety.
FIELD OF THE INVENTION
The present disclosure relates to a pesticidal mixture for pest control.
BACKGROUND OF THE INVENTION
Active agent mixtures are described in the literature. However, the control over the pests does not always satisfy the needs of agriculture practice. Additionally, the efficacy of mixtures are not entirely satisfactory in areas of pest control and/or toxicological and/or environmental effects.
Spinetoram is a semi-synthetic spinosyn. Spinetoram is a mixture of 50-90% (2R,3aR,5aR,5bS,9S,13S,14R,16aS,16bR)-2-(6-deoxy-3-O-ethyl-2,4-di-O-methyl-α-L-mannopyranosyloxy)-13-[(2R,5S,6R)-5-(dimethylamino)tetrahydro-6-methylpyran-2-yloxy]-9-ethyl-2,3,3a,4,5,5a,5b,6,9,10,11,12,13,14,16a,16b-hexadecahydro-14-methyl-1H-as-indaceno[3,2-d]oxacyclododecine-7,15-dione and 50-10% (2S,3aR,5aS,5bS,9S,13S,14R,16aS,16bS)-2-(6-deoxy-3-O-ethyl-2,4-di-O-methyl-α-L-mannopyranosyloxy)-13-[(2R,5S,6R)-5-(dimethylamino)tetrahydro-6-methylpyran-2-yloxy]-9-ethyl-2,3,3a,5a,5b,6,9,10,11,12,13,14,16a,16b-tetradecahydro-4,14-dimethyl-1H-as-indaceno[3,2-d]oxacyclododecine-7,15-dione.
Spinetoram has been described in U.S. Pat. No. 6,001,981. Spinetoram is also described in The E-Pesticide Manual (Version 5.0.1, 2010, 15th Edition, Editor: C D S Tomlin)), Entry number 783.




Spinetoram: Mixture of 50-90% component (i); and 50-10% component (ii).
Pesticidal mixtures comprising spinosyns have been described in WO 2010/052129, WO2010/030501, U.S. Pat. Nos. 6,444,667, 6,686,387 and US2006/0211655.
Pesticidal compositions and mixtures do not exert a satisfactory controlling effect in some cases, and therefore, there is still a need for development of new pesticidal mixtures having a satisfactory controlling effect.
Thus, it will be highly advantageous to have new pesticidal mixture combinations of spinetoram with various pesticides described in the invention, having a very good controlling effect on various pests, improving control over the pests, broadening the spectrum of control, and/or maintaining favorable and reduced environmental or toxicological effects.
SUMMARY OF THE INVENTION
According to one aspect there is provided a pesticidal mixture comprising (a) spinetoram; and (b) a compound of group A selected from novaluron. pyriproxyfen, tau-fluvalinate, and a combination thereof.
According to another aspect there is provided a pesticidal composition comprising a pesticidal mixture comprising (a) spinetoram.; and (b) a compound of group A selected from novaluron, pyriproxyfen, tau-fluvalinate, and a combination thereof; and an agriculturally acceptable carrier.
According to additional aspect there is provided a method for controlling pests in a crop, comprising contacting the pests or a locus, where control of the pest is desired, with a pesticidal mixture comprising (a) spinetoram; and (b) a compound of group A selected from novaluron, pyriproxyfen, tau-fluvalinate, and a combination thereof.
According to further aspect there is provided a kit comprising (a) at least one container including spinetoram; (b) at least one container including a compound of group A selected from novaluron, pyriproxyfen, tau-fluvalinate, and a combination thereof; and (c) instructions for applying said spinetoram and said compound of group A onto a pest or a locus, where control of the pest is desired.
According to still further aspect there is provided a kit comprising (a) at least one container including (i) spinetoram; and (ii) a compound of group A selected from novaluron, pyriproxyfen, tau-fluvalinate, and a combination thereof; and (b) instructions for applying said spinetoram and said a compound of group A onto a pest or a locus, where control of the pest is desired.


DETAILED DESCRIPTION OF THE INVENTION
Prior to setting forth the invention in detail, it may be helpful to provide definitions of certain terms to be used herein. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as is commonly understood by one of skill in the art to which this invention belongs.
The use of the terms “a” and “an” and “the” and similar referents in the context of describing the invention (especially in the context of the following claims) are to be construed to cover both the singular and the plural, unless otherwise indicated herein or clearly contradicted by context.
As used herein the term “crop” includes reference to whole plant, plant organ (e.g., leaves, stems, twigs, roots, trunks, limbs, shoots, fruits etc.), or plant cells.
As used herein the term “propagation material” is to he understood to denote all the generative parts of the plant such as seeds and spores, vegetative structures such as bulbs, corms, tubers, rhizomes, roots stems, basal shoots, stolons, and buds.
According to one aspect there is provided a pesticidal mixture comprising (a) spinetoram; and (h) a compound of group A selected from novaluron, pyriproxyfen, tau-fluvalinate, and a combination thereof.
Spinetoram is a mixture of 50-90% (2R,3aR,5aR,5bS,9S,13S,14R,16aS,16bR)-2-(6-deoxy-3-O-ethyl-2,4-di-O-methyl-α-L-mannopyranosyloxy)-13-[2R,5S,6R)-5-(dimethylamino)tetrahydro-6-methylpyran-2-yloxy]-9-ethyl-2,3,3a4,5,5a,5b,6,9,10,11,12,13,14,16a,16b-hexadecahydro-14-methyl-1H-as-indaceno[3,2-d]oxacyclododecine-7,15-dione and 50-10% (2S,3aR,5aS,5bS,9S,13S,14R,16aS,16bS)-2-(6-deoxy-3-O-ethyl-2,4-di-O-methyl-α-t-mannopyranosyloxy)-13-[(2R,5S,6R)-5-(dimethylamino)tetrahydro-6-methylpyran-2-yloxy]-9-ethyl-2,3,3a,5a,5b,6,9,10,11,12,13,14,16a,16b-tetradecahydro-4,14-dimethyl-1H-as-indaceno[3,2-d]oxacyclododecine-7,15-dione.
Spinetoram is described in The E-Pesticide Manual (Version 5.0.1, 2010, 15th Edition, Editor: C D S Tomlin)), Entry number 783.




Spinetoram; Mixture of 50-90% component 1); and 50-10% component (ii).
Novaluron ((±)-1-[3-chloro-4-(1,1,2-trifluoro-2-trifluoromethoxyethoxy)phenyl]-3-(2,6-difluorobenzoyl)urea),




is described in The E-Pesticide Manual (Version 5.0.1, 2010, 15th Edition, Editor: C D S Tomlin)), Entry number 621.
Tau-fluvalinate ((RS)-α-cyano-3-phenoxybenzyl N-(2-chloro-α,α,α-trifluoro-p-tolyl)-D-valinate),




is described in The E-Pesticide Manual (Version 5.0.1, 2010, 15th Edition, Editor: C D S Tomlin)) Entry number 423, and
Pyriproxyfen (4-phenoxyphenyl(RS)-2-(2-pyridyloxy)propyl ether)




is described in The E-Pesticide Manual (Version 5.0.1, 2010, 15th Edition, Editor: C D S Tomlin)) Entry number 749.
According to another aspect there is provided a pesticidal composition comprising a pesticidal mixture as described in the present disclosure; and an agriculturally acceptable carrier.
According to certain embodiments, the pesticidal mixture may result in a synergistic pesticidal activity when applied to pests.
Synergy can be calculated according to Colby S. R. in an article entitled “Calculating synergistic and antagonistic responses of herbicide combinations” published in the journal Weeds, 1967, 15, p. 20-22, incorporated herein by reference in its entirety.
A pesticidal composition comprising a mixture as described in the present disclosure may improve effectiveness of the pesticidal activity, may broaden the spectrum of control and/or allow minimizing the dosages of the active ingredients being used when compared to the use of such individual pesticides alone.
According to a specific embodiment the pest is selected from an insect, a mite, molluscs, or a combination thereof.
In a specific embodiment the pest is in an insect. In a specific embodiment the pest is in a mite. In a specific embodiment the pest is in a molluscs.
The term “pesticidal” especially relates to insecticidal and/or miticidal activities. The term “pesticidal” also refers to molluscicidal activity.
According to an additional aspect there is provided a synergistic pesticidal mixture comprising (a) spinetoram; and (b) a compound of group A selected from novaluron, pyriproxyfen, tau-fluvalinate, and a combination thereof.
According to an additional aspect there is provided a pesticidal composition comprising a pesticidal mixture as described in the present disclosure in a synergistically effective amount; and an agriculturally acceptable carrier.
The composition may be for use in controlling agricultural pests in a crop or a locus thereof comprising contacting the agricultural pests or the agricultural pests' food supply, habitat, breeding grounds, or locus with an effective amount of a mixture as described in the present disclosure.
According to a further aspect there is provided a method for controlling pests in a crop, comprising contacting the pests or a locus, where control of the pest is desired, with a pesticidal mixture comprising (a) spinetoram; and (b) a compound of group A selected from novaluron, pyriproxyfen, tau-fluvalinate, and a combination thereof.
According to a further aspect there is provided a method for controlling pests in a crop, comprising contacting the pests or a locus, where control of the pest is desired, with a composition comprising a pesticidal mixture as described in the present disclosure.
The locus may be the soil or water in which the crop is growing.
The disclosure additionally relates to a method for controlling pests in a crop or in a propagation material thereof, comprising contacting the pests or the agricultural pests' food supply, habitat, breeding grounds or locus with an amount of a combination of a pesticidal mixture as described in the present disclosure.
According to certain embodiments the ratio by weight of spinetoram and a compound of group A selected from novaluron, pyriproxyfen, tau-fluvalinate, and a combination thereof is from 1:1000 to 1000:1. In an embodiment the ratio by weight of spinetoram and a compound of group A is from 1:100 to 100:1. In a further embodiment the ratio by weight of spinetoram and a compound of group A is from 1:50 to 50:1. In still further embodiment the ratio by weight of spinetoram and a compound of group A is from 1:10 to 10:1. In an additional embodiment the ratio by weight of spinetoram and a compound of group A is from 1:8 to 8:1. In an additional embodiment the ratio by weight of spinetoram and a compound of group A is from 1:5 to 5:1.
According to some embodiments the ratio by weight of spinetoram and a compound of group A selected from novaluron, pyriproxyfen, tau-fluvalinate, and a combination thereof is 100:1 and below, 50:1 and below, 10:1 and below, 8:1 and below, 5:1 and below, 4:1 and below, 3:1 and below, 2:1 and below, 1.3:1 and below, 1:1 and below. According to some embodiments the ratio by weight of spinetoram and a compound of group A selected from novaluron, pyriproxyfen, tau-fluvalinate, and a combination thereof is 1:100 and above, 1:50 and above, 1:10 and above, 1:8 and above, 1:5 and above, 1:4 and above, 1:3 and above, 1:2 and above, 1:1.3 and above, 1:1 and above.
The ratio by weight of spinetoram and a compound of group A selected from novaluron, pyriproxyfen, tau-fluvalinate, and a combination thereof may be in a range between any of the above indicated values (e.g. 4:1 to 1:4, 4:1 to 1:3, 4:1 to 1:2, 4:1 to 1:1.3, 4:1 to 1:1, 3:1 to 1:4, etc.).
As used herein the terms “ha” refers to hectare.
According to certain embodiments, the application rates of spinetoram are from 0.5 gram per hectare (“g/ha”) to 500 g/ha, specifically from 1 g/ha to 500 g/ha, specifically from 1 g/ha to 250 g/ha, more specifically from 1 g/ha. to 100 g/ha.
According to certain embodiments, the application rates of spinetoram are 0.5 g/ha and above, 1 g/ha and above, 1.5 g/ha and above, 2 g/ha and above, 2.5 g/ha and above, 3 g/ha and above, 3.5 g/ha and above, 4 g/ha and above, 4.5 g/ha and above, 5 g/ha and above, 5.5 g/ha and above, 6 g/ha and above.
According to certain embodiments, the application rates of spinetoram are 500 g/ha and below, 250 g/ha and below, 200 g/ha and below, 150 g/ha and below, 100 g/ha and below, 50 g/ha and below, 20 g/ha and below, 10 g/ha and below, 8 g/ha and below.
The application rate of spinetoram may be any range between any of the above indicated values.
According to certain embodiments, the application rates of a compound of group A are from 0.5 g/ha to 500 g/ha, specifically from 1 g/ha to 500 g/ha, specifically from 1 g/ha. to 250 g/ha, more specifically from 1 g/ha to 100 g/ha.
According to certain embodiments, the application rates of a compound of group A are 0.5 g/ha and above, 1 g/ha and above, 1.5 g/ha and above, 2 g/ha and above, 2.5 g/ha and above, 3 g/ha and above, 3.5 g/ha and above, 4 g/ha and above, 4.5 g/ha and above, 5 g/ha and above, 5.5 g/ha and above, 6 g/ha and above.
According to certain embodiments, the application rates of a compound of group A are 500 g/ha and below, 250 g/ha. and below, 200 g/ha and below, 150 g/ha and below, 100 g/ha and below, 50 g/ha and below, 20 g/ha and below, 10 g/ha and below, 8 g/ha and below.
The application rate of a compound of group A selected from novaluron, pyriproxyfen, tau-fluvalinate, may be any range between any of the above indicated values.
According to certain embodiments the application rates of the pesticidal mixture as described in the present disclosure are from 1 g/ha to 1000 g/ha. In a specific embodiment the application rates of the pesticidal mixture as described in the present disclosure are from 2 g/ha to 1000 g/ha.
In another embodiment, the application rates of the combination (mixture) of spinetoram and a compound of group A are from 1 g/ha to 500 g/ha. In yet another embodiment, the application rates of the combination of spinetoram and a compound of group A are from 1 g/ha to 250 g/ha. In yet another embodiment, the application rates of the combination of spinetoram and a compound of group A are from 1 g/ha to 200 g/ha. In yet another embodiment, the application rates of the combination of spinetoram and a compound of group A are from 1 g/ha to 150 g/ha. In yet another embodiment, the application rates of the combination of spinetoram and a compound of group A are from 1 g/ha to 100 g/ha. In yet another embodiment, the application rates of the combination of spinetoram and a compound of group A are from 1 g/ha to 50 g/ha. The above ranges refer to the application rates of a combination of spinetoram. and a compound of group A (namely a sum of the application rates of both active ingredients).
In an embodiment the spinetoram and the compound of group A are applied concomitantly or sequentially.
The application of spinetoram and the compound of group A may be in any desired order.
The term “applied sequentially” means the successive administration of a composition including spinetoram, and then administration of a composition of a compound of group A. When administered sequentially the spinetoram and the compound of group A are included in separate compositions. If the spinetoram and the compound of group A are administered sequentially, the order of administering thereof may be interchangeable.
The term “applied simultaneously” or “applied concomitantly” means administering the compounds substantially concurrently. These terms encompasses not only administering spinetoram and the compound of group A according to the invention in a single formulation (composition) but also the administration of each compound (i.e spinetoram and the compound of group A) in its own separate formulation. Where separate formulations are used, the compounds can be administered at essentially the same time, i.e., concurrently.
The application may be by any method known in the art, particularly, by methods such as by spraying, dipping, dusting, dressing, coating, and soaking.
The disclosure additionally relates to use of a composition comprising a pesticidal mixture as described in the present disclosure for controlling pests in a crop or locus thereof.
The disclosure further relates to a composition comprising a pesticidal mixture as described in the present disclosure for use in controlling pests in a crop or locus thereof. In a specific embodiment the pesticidal mixture is in a synergistic effective amount.
The crops include but not limited to vegetables, fruits, soybean, tobacco, cultivated mushrooms, cotton, oil seed rape, cereals, sunflower, peanuts, rice, corn, coffee, beans, peas, yucca, sugar cane, clover, spices, ornamentals, cocoa, beet.
In an embodiment, the crop may be vegetables, such as peppers, cabbage, broccoli, asparagus, artichoke, squash, lettuce, turnip, spinach, cauliflower, melon, watermelon, cucumbers, carrots, onions, garlic, cucurbits and potatoes; fruits for example, pome and stone fruits and berries, such as walnuts, kiwi, banana, avocado, olives, passion fruit, almonds, pineapples, apples, pears, plums, peaches, and cherries, tropical and subtropical fruits, table and wine grapes, citrus fruit, such as oranges, lemons, grapefruits and limes; cereals such as wheat, barley, rye, triticale, oats, sorghum; corn (maize) such as sweet corn; beet such as sugar beet, fodder beet; spices such as cardamom; ornamentals such as flowers, Christmas trees.
In another embodiment, the insect pests are of the order Coleoptera, such as Acanthoscelides spp. (weevils), Acanthoscelides obtectus (common bean weevil), Agrilus planipennis (emerald ash borer), Agriotes spp. (wireworms), Anoplophora glabripennis (Asian longhorned beetle), Anthonomus spp. (weevils), Anthonomus grandis (boll weevil), Aphidius spp., Apion spp. (weevils), Apogonia spp. (grubs), Ataenius spretulus (Black Turgrass Ataenius), Atomaria linearis (pygmy mangold beetle). Aulacophore spp., Bothynoderes punctiventris (beet root weevil), Bruchus spp. (weevils), Bruchus pisorum (pea weevil), Cacoesia spp., Callosobruchus maculatus (southern cow pea weevil), Carpophilus hemipteras (dried fruit beetle), Cassida vittata, Cerosterna spp, Cerotoma spp. (chrysomeids), Cerotoma trifurcata (bean leaf beetle), Ceutorhynchus spp. (weevils), Ceutorhynchus assimilis (cabbage seedpod weevil), Ceutorhynchus napi (cabbage curculio), Chaetocnema spp. (chrysomelids), Colaspis spp. (soil beetles), Conoderus scalaris, Conoderus stigmosus, Conotrachelus nenuphar (plum curculio), Cotinus nitidis (Green June beetle). Crioceris asparagi (asparagus beetle), Cryptolestes ferrugineus (rusty grain beetle), Cryptolestes pusillus (flat grain beetle), Cryptolestes turcicus (Turkish grain beetle), Ctenicera spp. (wireworms), Curculio spp. (weevils), Cyclocephala spp. (grubs), Cylindrocpturus adspersus (sunflower stem weevil), Deporaus marginatus (mango leaf-cutting weevil), Dermestes lardarius (larder beetle), Dermestes maculates (hide beetle), Diabrotica spp. (chrysolemids), Epilachna varivestis (Mexican bean beetle), Faustinus cubae, Hylobius pales (pales weevil), Hypera spp. (weevils), Hypera postica (alfalfa weevil), Hyperdoes spp. (Hyperodes weevil), Hypothenemus hampei (coffee berry beetle), Ips spp. (engravers), Lasioderma serricorne (cigarette beetle), Leptinotarsa decemlineata (Colorado potato beetle), Liogenys futscus, Liogenys suturalis, Lissorhoptrus oryzophilus (rice water weevil), Lyctus spp. (wood beetles/powder post beetles), Maecolaspis joliveti, Megascelis spp., Melanotus communis, Meligethes spp., Meligethes aeneus (blossom beetle), Melolontha melolontha (common European cockchafer), Oberea brevis, Oberea linearis, Oryctes rhinoceros (date palm beetle), Oryzaephilus mercator (merchant grain beetle), Oryzaephilus surinamensis (sawtoothed grain beetle), Otiorhynchus spp. (weevils), Oulema melanopus (cereal leaf beetle), Oulema oryzae, Pantomorus spp. (weevils), Phyllophaga spp. (May/June beetle), Phyllophaga cuyabana, Phyllotreta spp. (chrysomelids), Phynchites spp., Popillia japonica (Japanese beetle), Prostephanus truncates (larger grain borer), Rhizopertha dominica (lesser grain borer), Rhizotrogus spp. (Eurpoean chafer), Rhynchophorus spp. (weevils), Scolytus spp. (wood beetles), Shenophorus spp. (Billbug), Sitona lineatus (pea leaf weevil), Sitophilus spp. (grain weevils), Sitophilus granaries (granary weevil), Sitophilus oryzae (rice weevil), Stegobium paniceum (drugstore beetle), Tribolium spp. (flour beetles), Tribolium castaneum (red flour beetle), Tribolium confusum (confused flour beetle), Trogoderma variabile (warehouse beetle) and Zabrus tenebioides. 
In yet another embodiment, the insect pests are of the order Diptera, such as Aedes spp. (mosquitoes) Agromyza frontella (alfalfa blotch leafminer), Agromyza spp. (leaf miner flies). Anastrepha spp. (fruit flies), Anastrepha suspensa (Caribbean fruit fly), Anopheles spp. (mosquitoes), Batrocera spp. (fruit flies), Bactrocera cucurbitae (melon fly), Bactrocera dorsalis (oriental fruit fly), Ceratitis spp. (fruit flies), Ceratitis capitata (Mediterranea fruit fly), Chrysops spp. (deer flies), Cocliliamyia spp. (screwworms), Contarinia spp. (Gall midges), Culex spp. (mosquitoes), Dasineura spp. (gall midges), Dasineura brassicae (cabbage gall midge), Delia spp., Delia platura (seedcorn maggot), Drosophila spp. (vinegar flies), Fannia spp. (filth flies), Fannia canicularis (little house fly), Fannia scalaris (latrine fly), Gasterophilus intestinalis (horse bot fly), Gracillia perseae, Haematobia irritans (horn fly), Hylemyia spp. (root maggots), Hypoderma lineatum (common cattle grub), Liriomyza spp. (leafminer flies), Liriomyza brassica (serpentine leafminer), Melophagus ovinus (sheep ked), Musca spp. (muscid Musca autumnalis (face fly), Musca domestica (house fly), Oestrus ovis (sheep bot fly), Oscinella frit (grass fly), Pegomyia betae (beet leafminer), Phorbia spp., Psila rosae (carrot rust fly), Rhagoletis cerasi (cherry fruit fly), Rhagoletis pomonella (apple maggot), Sitodiplosis mosellana (orange wheat blossom midge), Stomoxys calcitrans (stable fly), Tabanus spp. (horse flies) and Tipula spp. (crane flies).
In yet another embodiment, the insect pests are of the order Hemiptera, such as Acrostemum hilare (green stink bug), Blissus leucopterus (chinch bug), Calocoris norvegicus (potato mirid), Cimex hemipterus (tropical bed bug), Cimex lectularius (bed bug), Dichelops melacanthus (Dallas), Dagbertus fasciatus, Dichelops furcatus, Dysdercus suturellus (cotton stainer), Edessa meditabunda, Eurygaster maura (cereal bug), Euschistus heros, Euschistus serous (brown stink bug), Helopeltis antonii, Helopeltis theivora (tea blight plantbug), Lagynotomus spp. (stink bugs), Leptocorisa oratorius, Leptocorisa varicomis, Lygus spp. (plant bugs), Lygus hesperus (western tarnished plant bug), Maconellicoccus hirsutus, Neurocolpus longirostris, Nezara viridula (southern green stink bug), Paratrioza cockerelli, Phytocoris spp. (plant bugs), Phytocoris califomicus, Phytocoris relativus, Piezodorus guildingi, Poecilocapsus lineatus (fourlined plant bug), Psallus vaccinicola, Pseudacysta perseae, Scaptocoris castanea and Triatoma spp. (bloodsucking conenose bugs/kissing bugs).
In yet another embodiment, the insect pests are of the order Homoptera, such as Acrythosiphon pisum (pea aphid), Adelges spp. (adelgids), Aleurodes proletella (cabbage whitefly), Aleurodicus disperses, Aleurothrixus floccosus (woolly whitefly), Aluacaspis spp. Amrasca bigutella bigutella, Aphrophora spp. (leafhoppers), Aonidiella aurantii (California red scale), Aphis spp. (aphids), Aphis gossypii (cotton aphid), Aphis fabae (aphid), Aphis pomi (apple aphid), Aulacorthum solani (foxglove aphid), Bemisia spp. (whiteflies), Bemisia argentifolii, Bemisia tabaci (sweet potato whitefly), Brachycolus noxius (Russian aphid), Brachycoryneila asparagi (asparagus aphid), Brevennia rehi, Brevicoryne brassicae (cabbage aphid), Ceroplastes spp. (scales), Ceroplastes rubens (red wax scale), Chionaspis spp. (scales), Chrysomphalus spp. (scales), Coccus spp. (scales), Dysaphis plantaginea (rosy apple aphid), Empoasca spp. (leafhoppers), Eriosoma lanigerum (woolly apple aphid), Icerya purchasi (cottony cushion scale), Idioscopus nitidulus (mango leafhopper), Laodelphax striatellus (smaller brown planthopper), Lepidosaphes spp., Macrosiphum spp., Macrosiphum euphorbiae (potato aphid), Macrosiphum granarium (English grain aphid), Macrosiphum rosae (rose aphid), Macrosteles quadrilineatus (aster leafhopper), Mahanarva frimbiolata, Metopolophiurn dirhodurn (rose grain aphid), Mictis longicornis, Myzus persicae (green peach aphid), Nephotettix spp. (leafhoppers), Nephotettix cinctipes (green leafhopper), Nilaparvata lugens (brown planthopper), Parlatoria pergandii (chaff scale), Parlatoria ziziphi (ebony scale), Peregrinus maidis (corn delphacid), Philaenus spp. (spittlebugs), Phylloxera vitifoliae (grape phylloxera), Physokermes piceae (spruce bud scale), Planococcus spp. (mealybugs), Pseudococcus spp. (mealybugs), Pseudococcus brevipes (pine apple mealybug), Quadraspidiotus perniciosus (San Jose scale), Rhapalosiphum spp. (aphids), Rhapalosiphum maida (corn leaf aphid), Rhapalosiphum padi (oat bird-cherry aphid), Saissetia spp. (scales), Saissetia oleae (black scale), Schizaphis graminum (greenbug), Sitobion avenae (English grain aphid), Sogatella furcifera (white-backed planthopper), Therioaphis spp. (aphids), Toumeyella spp. (scales), Toxoptera spp. (aphids), Trialeurodes spp. (whiteflies), Trialeurodes vaporariorum (greenhouse whitefly), Trialeurodes abutiloneus (banded wing whitefly), Unaspis spp. (scales), Unaspis yanonensis (arrowhead scale) and Zulia entreriana. 
In yet another embodiment, the insect pests are of the order Lepidopter such as Achoea Janata, Adoxophyes spp., Adoxophyes orana, Agrotis spp. (cutworms), Agrotis ipsilon (black cutworm), Alabama argillacea (cotton leafworm), Amorbia cuneana, Amyelosis transitella (navel orangeworm), Anacamptodes defectaria, Anarsia lineatella (peach twig borer), Anomis sabulifera (jute looper), Anticarsia gemmatalis (velvetbean caterpillar), Archips argyrospila (fruittree leafroller) Archips rosana (rose leaf roller), Argyrotaenia spp. (tortricid moths), Argyrotaenia citrine (orange torrid), Autograph gamma, Bongos crunodes, Bourbon cinnabar (rice leaf folder), Bucculatrix thurheriella (cotton leafperforator), Caloptilia spp. (leaf miners), Capua reticulana, Carposina niponensis (peach fruit moth), Chilo spp., Chlumetia transversa (mango shoot borer), Choristoneura rosaceana (obliquebanded leafroller), Chrysodeixis spp., Cnaphalocerus medinalis (grass leafroller), Colias spp., Conpomorpha cramerella, Cossus cossus (carpenter moth), Crambus spp. (Sod webworms), Cydia funebrana (plum fruit moth), Cydia molesta (oriental fruit moth), Cydia nignicana (pea moth), Cydia pomonella (codling moth), Darna diducta, Diaphania spp. (stem borers), Diatraea spp. (stalk borers), Diatraea saccharalis (sugarcane borer), Diatraea graniosella (southwester corn borer), Earias spp. (bollworms), Earias insulata (Egyptian bollworm), Earias vitella (rough northern bollworm), Ecdytopopha aurantianum, Elasmopalpus lignosellus (lesser cornstalk borer), Epiphysias postruttana (light brown apple moth), Ephestia spp. (flour moths), Ephestia cautella (almond moth), Ephestia elutella (tobbaco moth), Ephestia kuehniella (Mediterranean flour moth), Epimeces spp., Epinotia aporema, Erionota thrax (banana skipper), Eupoecilia ambiguella (grape berry moth), Euxoa auxiliaris (army cutworm), Feltia spp. (cutworms), Gortyna spp. (stemborers), Grapholita molesta (oriental fruit moth), Fledylepta indicata (bean leaf webber), Helicoverpa spp. (noctuid moths), Helicoverpa armigera (cotton bollworm), Helicoverpa zea (bollworm/corn earworm), Heliothis spp. (noctuid moths), Heliothis virescens (tobacco budworin), Hellula undalis (cabbage webworm), Indarbela spp. (root borers), Keiferia lycopersicella (tomato pinworm), Leucinodes orbonalis (eggplant fruit borer), Leucoptera malifoliella, Lithocollectis spp., Lobesia botrana (grape fruit moth), Loxagrotis spp. (noctuid moths), Loxagrotis albicosta (western bean cutworm), Lymantria dispar (gypsy moth), Lyonetia clerkella (apple leaf miner), Mahasena corbetti (oil palm bagworin), Malacosoma spp. (tent caterpillars), Mamestra brassicae (cabbage armyworm), Maruca testulalis (bean pod borer), Metisa plana (bagworm), Mythimna unipuncta (true armyworm), Neoleucinodes elegantalis (small tomato borer), Nymphula depunctalis (rice caseworm), Operophthera brumata (winter moth), Ostrinia nubilalis (European corn borer), Oxydia vesulia, Pandemis cerasana (common currant tortrix), Pandemis heparana (brown apple tortrix), Papilio demodocus, Pectinophora gossypiella (pink bollworm), Peridroma spp. (cutworms), Peridroma saucia (variegated cutworm), Perileucoptera coffeella (white coffee leafminer), Phthorimaea operculella (potato tuber moth), Phyllocnisitis arena, Phyllonorycter spp. (leafminers), Pieris rapae (imported cabbageworm), Plathypena scabra, Plodia interpunctella (Indian meal moth), Plutella xylostella (diamondback moth), Polychrosis viteana (grape berry moth), Prays endocarpa, Prays oleae (olive moth), Pseudaletia spp. (noctuid moths), Pseudaletia unipunctata (armyworm), Pseudoplusia includens (soybean looper), Rachiplusia Scirpophaga incertulas, Sesamia spp. (stemborers), Sesamia inferens (pink rice stem borer), Sesamia nonagrioides, Setora nitens, Sitotroga cerealella (Angoumois grain moth), Sparganothis pilleriana, Spodoptera spp. (armyworms), Spodoptera exigua (beet armyworm), Spodoptera fugiperda (fall armyworm), Spodoptera littoralis (cotton leafworm), Spodoptera oridania (southern armyworm), Synanthedon spp. (root borers), Thecla basilides, Thermisia gemmatalis, Tineola bisselliella (webbing clothes moth), Trichoplusia ni (cabbage looper), Tuta absoluta, Yponometita spp., Zeuzera coffeae (red branch borer) and Zeuzera pyrina (leopard moth).
In yet another embodiment, the insect pests are of the order Orthoptera, such as Anabrus simplex (Mormon cricket), Gryllotalpidae (mole crickets), Locusta migratoria, Melanoplus spp. (grasshoppers), Microcentrum retinerve (angularwinged katydid), Pterophylla spp. (kaydids), chistocerca gregaria, Scudderia furcata (forktailed bush katydid) and Valanga nigricorni. 
In yet another embodiment, the insect pests are of the order Thysanoptera, such as Frankliniella fusca (tobacco thrips), Frankliniella occidentalis (western flower thrips), Frankliniella shultzei Frankliniella williamsi (corn thrips), Heliothrips haemorrhaidalis (greenhouse thrips), Riphiphorothrips cruentatus, Scirtothrips spp., Scirtothrips citri (citrus thrips), Scirothrips dorsalis (yellow tea thrips), Taeniothrips rhopalantennalis and Thrips spp.
The mites may be for example, Red Spider mite (Tetranychus urticae), European red mite (Panonvchus ulmi) Citrus Red mite (Panonychus citri) Rust Mite (Aceria anthocoptes), Citrus Flat mite (Brevipalpus lewisi), False Spider mite (Brevipalpus phoenicis), Yellow Mite (Lorryia Formosa), Brown Mite (Bryobia rubrioculus), Peach silver mite (Aculus cornutus), Two Spotted Spider mite (Tetranychus urticae Koch).
The Molluscs may be for example Grey garden slug (Deroceras reticulatum), Spanish slug (Arion vulgaris), Yellow slug (Limax flavus), Tree slug (Limax marginatus), Spanish Stealth slug (Arion Flagellus), Common Garden slug (Arion distinctus), Common Keeled slug (Tandonia budapestensis), Large Red slug and Black slug (Arion ater), Great grey slug (Limax maximus), Portuguese slug (Arion lusitanicus).
The application rates for the pesticidal compositions of spinetoram and a compound of group A can be influenced by many factors of the environment. The desired effect and can be determined under actual use conditions.
The composition may be applied in various mixtures or combinations of spinetoram and a compound of group A, for example in a single “ready-for-use” form, or in a combined spray mixture composed from separate formulations of the single active ingredients, such as a “tank-mix” form.
In certain embodiments, the composition is applied in the form of a ready-for-use formulation comprising spinetoram and a compound of group A. This formulation can be obtained by combining the mixture described in the present disclosure with an agriculturally acceptable carrier, a surfactant, or other application-promoting adjuvant customarily employed in formulation technology.
Compositions comprising (a) spinetoram and (b) a compound of group A may be employed in any conventional form, for example, in the form of a twin pack. The compositions may be in a form of, for example, an emulsion concentrates (“EC”), suspension concentrates (“SC”), soluble concentrates (“SL”), suspoemulsion (“SE”), oil dispersions (“OD”), water dispersible granules (“WDG” or “WG”), water soluble granules (“SG”) and wettable powders (“WP”). Such compositions can be formulated using agriculturally acceptable carriers, surfactants or other application-promoting adjuvants customarily employed in formulation technology and formulation techniques that are known in the art.
In an embodiment, the amount of the active ingredient or ingredients in the composition is 0.01-99.9 percent by weight (“wt. %”), specifically 0.1-99 wt. %, more specifically 0.1-95 wt. %, even more specifically 0.1-90 wt. %, based on the total weight of the composition.
Examples of suitable solid carriers include mineral earths such as silica gels, silicates, talc, kaolin, attaclay, limestone, lime, chalk, bole, loess, clay, dolomite, diatomaceous earth, calcium sulfate, magnesium sulfate, magnesium oxide, sodium carbonate and bicarbonate, and sodium sulfate, ground synthetic materials, fertilizers, such as, for example, ammonium sulfate, ammonium phosphate, ammonium nitrate, ureas, and products of vegetable origin, such as cereal meal, tree bark meal, wood meal and nutshell meal, cellulose powders and other solid carriers.
Examples of suitable liquid carriers include water, alcohols such as methanol, cyclohexanol and decanol, ethylene glycol and polypropylene glycol, N,N-dimethylformamide, dimethylsulfoxide, N-alkylpyrrolidone, aromatic hydrocarbons such as alkylbenzenes and alkylnaphthalenes, paraffins, oils of olive, castor, linseed, tong, sesame, corn, peanut, cotton-seed, soybean, rape-seed and coconut, fatty acid esters, ketones such as cyclohexanone, 2-heptanone, isophorone and 4-hydroxy-4-methyl-2-pentanone and the like.
The term “surfactant,” as used herein, means an agriculturally acceptable material which imparts emulsifiability, stability, spreading, wetting, dispersibility or other surface-modifying properties. Examples of suitable surfactants include non-ionic, anionic, cationic and ampholytic types such as lignin sulfonates, fatty acid sulfonates lauryl sulfonate), phosphate esters such as phosphate esters of alcohol alkoxylates, phosphate esters of alkylphenol alkoxylates and phosphate esters of styrylphenol ethoxylates, condensates of sulfonated naphthalene and naphthalene derivatives with formaldehyde, condensates of naphthalene or of naphthalenesulfonic acid with phenol and formaldehyde, alkylarylsulfonates, ethoxylated alkylphenols and aryl phenols, polyalkylene glycols and ethoxylated fatty alcohols.
Other ingredients, such as wetting agents, adhesives, thickeners, hinders, fertilizers or anti-freeze agents, may also be added to the composition including the combination of spinetoram and a compound of group A in order to increase the stability, density and viscosity of the composition.
In an embodiment, the combined amount of spinetoram and a compound of group A together in the ready-to-use formulations (compositions) is 0.1-99 wt. %.
In an embodiment, the combined amount of spinetoram and a compound of group A together in the ready-to-use formulations is 0.1-95 wt. %.
In another embodiment the amount of spinetoram and a compound of group A together in the ready-to-use formulations (compositions) is 10−4-99 wt. %, 10−3-99 wt. %, or 10−2-99 wt. In a specific embodiment the amount of spinetoram and a compound of group A together in the ready-to-use formulations (compositions) is 10−5-10 wt. %, 10−5-1 wt. %, 10−5-0.1 wt. %, 10−4-10 wt. %, 10−4-5 wt %, 10−4-2 wt %, 10−4-1 wt %, 10−4-0.5 wt %, or 10−4-0.1 wt %.
The remaining components in the formulation are for example t carrier and additives. In specific embodiment the carrier is aqueous based.
According to further aspect there is provided a kit comprising (a) at least one container including spinetoram; (b) at least one container including a compound of group A selected from novaluron, pyriproxyfen, tau-fluvalinate, and a combination thereof; and (c) instructions for applying said spinetoram and said compound of group A onto a pest or a locus, where control of the pest is desired.
In an embodiment the spinetoram and the compound of group A are applied concomitantly or sequentially.
According to still further aspect there is provided a kit comprising (a) at least one container including (i) spinetoram; and (ii) a compound of group A selected from novaluron, pyriproxyfen, tau-fluvalinate, and a combination thereof; and (b) instructions for applying said spinetoram and said compound of group A onto a pest or a locus, where control of the pest is desired.
The instructions may be in the form of printed matter, for example either as inserts or labels.
A synergistic effect exists wherever the action of a combination of active components is greater than the sum of the action of each of the components alone. Therefore, a synergistically effective amount (or an effective amount of a synergistic composition or combination) is an amount that exhibits greater pesticidal. activity than the sum of the pesticidal activities of the individual components.
In the field of agriculture, it is often understood that the term “synergy” is as defined by Colby S. R. in an article entitled “Calculating synergistic and antagonistic responses of herbicide combinations” published in the journal Weeds, 1967, 15, p. 20-22, incorporated herein by reference in its entirety. The action expected for a given combination of two active components can be calculated as follows:




  E
  =
  
    X
    +
    Y
    -
    
      XY
      100
    
  




in which E represents the expected percentage of pesticidal control for the combination of the two pesticides at defined doses (for example equal to x and y respectively), X is the percentage of pesticidal control observed by spinetoram at a defined dose (equal to x) and Y is the percentage of pesticidal control observed by a compound of group A at a defined dose (equal to y). When the percentage of pesticidal control observed for the combination is greater than the expected percentage, there is a synergistic effect.

It is appreciated that the spinetoram and the compound of group A, described in the invention in a particular aspect may be characterized by the various features, e.g., weight ratios, rates of applications etc., described in the present invention in the other aspects.
It is appreciated that one or more features, aspects, or embodiments of the present invention can be combined with one or more other features, aspects or embodiments of the present invention.
It is recognized that all embodiments of the invention, including those specifically described for different aspects of the invention, can be combined with any other embodiments of the invention as appropriate.
EXAMPLES
Example 1
Novaluron and Spinetoram in Control of Plutella xylostella on Cabbage (Brassica oleracea L Var. Capitata) and Broccoli (Brassica oleracea L Var. Italica)
Two trials—one on cabbage crops, and one on broccoli crops—were conducted in the district of La Plata (Buenos Aires, Argentina) with the goal of evaluating the efficacy of and interaction between foliar applications of Novaluron and Spinetoram in Plutella control. For preparation of the mixtures, Spinetoram (Delegate® 250 g a.i./Kg WG, Dow AgroSciences LLC) and Novaluron (Rimon® Supra 100 g a.i./Liter SC, Makhteshim Agan Group Company) were used. For each treatment in Examples 1-6 determined amounts of the formulated products were diluted with water to obtain a mixture.
Applications were made with a backpack-type sprayer fitted with a 2.1-m wide bar with nozzles set at 0.525 m from one another, with 8002 flat fan tips. In all cases, applications were made from 5 p.m. to 7 p.m. at a rate of 150 l·ha−1 (Liter/ha).
Ten-meter-long plots were used, each divided into four ridges. Applications were made only on three ridges. A one-ridge-wide buffer zone was left among plots to minimize drift. Experiment design consisted in random blocks at four repetitions.
Evaluation following 1, 3 and 7 days post-application is presented in Table B below. Larvae per plant (LP) was an average of 10 plants per plot. 4 repetitions were averaged. % Efficacy of Control in Examples 1-6 was calculated, based on untreated control evaluated on the same days.







TABLE A







Description of each trial.













Rate of





application



Location
Phenological Status
(L/ha)





Trial 1
Los Hornos (La Plata)
Vegetative
150 lts/ha


Trial 2
Etcheverry (La Plata)
Vegetative
150 lts/ha
















TABLE B





Results







Trial 1 - Broccoli











Novaluron
Spinetoram
% Efficacy of control















amount of
amount of
1
3
7




ai(*), g/ha
ai g/ha
DAA
DAA
DAA





1
Novaluron + Spinetoram
7.5
7.5
92.35%
100.00%
100.00%


2
Novaluron + Spinetoram
7.5
5.625
86.47%
100.00%
100.00%


3
Novaluron + Spinetoram
7.5
3.75
83.53%
96.88%
93.02%


4
Novaluron + Spinetoram
7.5
1.875
64.71%
78.13%
100.00%


5
Novaluron + Spinetoram
5.625
7.5
92.35%
98.13%
93.02%


6
Novaluron + Spinetoram
5.625
5.625
79.41%
100.00%
93.02%


7
Novaluron + Spinetoram
5.625
3.75
70.59%
98.13%
100.00%


8
Novaluron + Spinetoram
5.625
1.875
57.06%
93.75%
100.00%


9
Novaluron + Spinetoram
3.75
7.5
85.29%
100.00%
100.00%


10
Novaluron + Spinetoram
3.75
5.625
80.59%
91.88%
93.02%


11
Novaluron + Spinetoram
3.75
3.75
61.76%
87.50%
88.37%


12
Novaluron + Spinetoram
3.75
1.875
54.12%
75.00%
93.02%


13
Novaluron + Spinetoram
1.875
7.5
65.88%
84.38%
65.12%


14
Novaluron + Spinetoram
1.875
5.625
64.71%
76.25%
93.02%


15
Novaluron + Spinetoram
1.875
3.75
68.82%
60.63%
76.74%


16
Novaluron + Spinetoram
1.875
1.875
41.18%
65.63%
76.74%


17
Novaluron
7.5
0
47.06%
59.38%
88.37%


18
Spinetoram
0
7.5
85.29%
95.00%
88.37%


19
Novaluron
5.625
0
60.00%
50.00%
88.37%


20
Spinetoram
0
5.625
62.94%
82.50%
81.40%


21
Novaluron
3.75
0
60.00%
53.13%
76.74%


22
Spinetoram
0
3.75
62.94%
84.38%
93.02%


23
Novaluron
1.875
0
50.00%
35.63%
81.40%


24
Spinetoram
0
1.875
41.18%
54.38%
46.51%


25
Untreated controls (UTC)
0
0
0.00%
0.00%
0.00%










(*)In this table and tables below, the term “ai” refers to active ingredient.


Trial 2 - Cabbage











Novaluron
Spinetoram
% Efficacy of control















amount of
amount of
1
3
7




ai, g/ha
ai, g/ha
DAA
DAA
DAA





1
Novaluron+
7.5
7.5
94.30%
100.00%
100.00%


2
Novaluron+
7.5
5.625
87.45%
100.00%
100.00%


3
Novaluron+
7.5
3.75
86.69%
95.24%
97.46%


4
Novaluron+
7.5
1.875
69.58%
83.33%
95.76%


5
Novaluron+
5.625
7.5
88.59%
98.57%
100.00%


6
Novaluron+
5.625
5.625
85.55%
96.19%
100.00%


7
Novaluron+
5.625
3.75
74.14%
88.10%
88.98%


8
Novaluron+
5.625
1.875
75.29%
83.33%
88.98%


9
Novaluron+
3.75
7.5
77.95%
93.81%
100.00%


10
Novaluron+
3.75
5.625
80.99%
85.71%
93.22%


11
Novaluron+
3.75
3.75
77.95%
81.90%
88.98%


12
Novaluron+
3.75
1.875
61.98%
73.81%
70.34%


13
Novaluron+
1.875
7.5
77.95%
85.71%
87.29%


14
Novaluron+
1.875
5.625
71.48%
80.95%
83.05%


15
Novaluron+
1.875
3.75
68.44%
74.76%
72.03%


16
Novaluron+
1.875
1.875
62.74%
52.38%
55.08%


17
Novaluron
7.5
0
55.13%
57.14%
42.37%


18
Spinetoram
0
7.5
85.55%
73.81%
87.29%


19
Novaluron
5.625
0
70.34%
52.38%
59.32%


20
Spinetoram
0
5.625
72.24%
78.57%
100.00%


21
Novaluron
3.75
0
60.84%
57.14%
32.20%


22
Spinetoram
0
3.75
58.94%
66.67%
95.76%


23
Novaluron
1.875
0
63.88%
58.10%
8.47%


24
Spinetoram
0
1.875
67.68%
59.52%
83.05%


25
Untreated controls (UTC)
0
0
0.00%
0.00%
0.00%









Example 2
Novaluron and Spinetoram in Fall Armyworm (Spodoptera frugiperda) Control in Sweet Corn (Zea mays Var. Saccharata)
Two trials were conducted on sweet corn crops in the district of Orán (Salta, Argentina) with the goal of evaluating the efficacy and interaction of foliar applications of Novaluron and Spinetoram on fall armyworms (Spodoptera frugiperda). For preparation of the mixtures the products used are as described in Example 1.
Applications were made with a backpack type sprayer fitted with a 2.1-m wide bar with nozzles set at 0.525 in from one another and fitted with 8002 flat fan tips. In all cases, applications were made between 6.00 p.m. and 9.00 p.m. at a rate of 205 l·ha−1.
Ten-meter-long plots were used, each divided into four ridges. Applications were made only on three ridges. A one-ridge-wide buffer zone was left among plots to minimize drift. Experiment design consisted in random blocks at four repetitions.
The evaluation following 1, 3 and 7 days post-application is presented in Table B below. Larvae per plant (LP) was an average of 10 plants per plot (4 repetitions were averaged). % Efficacy was calculated in the same manner as described in Example 1.







TABLE A







Description of each trial













Rate of





application



Location
Phenological Status
(l · ha)





Trial 1
Rio Blanco (Oran, Salta)
V 5
205 lts/ha


Trial 2
Las Yungas (Oran, Salta)
V 5
205 lts/ha
















TABLE B







Results











amount of ai
amount of ai
% Efficacy of control













Novaluron,
Spinetoram,
1
3
7



g/ha
g/ha
DAA
DAA
DAA










Trial 1


% of Efficacy (according to number of larva/plant)













1
Novaluron + Spinetoram
7.5
7.5
97.78%
100.00%
100.00%


2
Novaluron + Spinetoram
7.5
5.625
95.56%
100.00%
100.00%


3
Novaluron + Spinetoram
7.5
3.75
95.56%
100.00%
100.00%


4
Novaluron + Spinetoram
7.5
1.875
82.22%
96.97%
100.00%


5
Novaluron + Spinetoram
5.625
7.5
100.00%
98.48%
100.00%


6
Novaluron + Spinetoram
5.625
5.625
100.00%
96.97%
97.01%


7
Novaluron + Spinetoram
5.625
3.75
88.89%
96.97%
98.51%


8
Novaluron + Spinetoram
5.625
1.875
93.33%
95.45%
92.54%


9
Novaluron + Spinetoram
3.75
7.5
84.44%
93.94%
91.04%


10
Novaluron + Spinetoram
3.75
5.625
68.89%
90.91%
86.57%


11
Novaluron + Spinetoram
3.75
3.75
64.44%
90.91%
83.58%


12
Novaluron + Spinetoram
3.75
1.875
57.78%
80.30%
85.07%


13
Novaluron + Spinetoram
1.875
7.5
55.56%
80.30%
80.60%


14
Novaluron + Spinetoram
1.875
5.625
51.11%
77.27%
68.66%


15
Novaluron + Spinetoram
1.875
3.75
42.22%
65.15%
64.18%


16
Novaluron + Spinetoram
1.875
1.875
31.11%
74.24%
52.24%


17
Novaluron
7.5
0
77.78%
93.94%
83.58%


18
Spinetoram
0
7.5
77.78%
96.97%
89.55%


19
Novaluron
5.625
0
66.67%
89.39%
76.12%


20
Spinetoram
0
5.625
71.11%
84.85%
76.12%


21
Novaluron
3.75
0
51.11%
78.79%
65.67%


22
Spinetoram
0
3.75
64.44%
78.79%
59.70%


23
Novaluron
1.875
0
35.56%
63.64%
46.27%


24
Spinetoram
0
1.875
44.44%
62.12%
50.75%


25
Untreated controls (UTC)
0
0
0.00%
0.00%
0.00%







Trial 2


% of Efficacy (according to number of larva/plant)













1
Novaluron + Spinetoram
7.5
7.5
68.42%
92.45%
100.00%


2
Novaluron + Spinetoram
7.5
5.625
68.42%
84.91%
100.00%


3
Novaluron + Spinetoram
7.5
3.75
62.28%
94.34%
98.28%


4
Novaluron + Spinetoram
7.5
1.875
60.53%
86.79%
93.10%


5
Novaluron + Spinetoram
5.625
7.5
63.16%
92.45%
100.00%


6
Novaluron + Spinetoram
5.625
5.625
63.16%
94.34%
98.28%


7
Novaluron + Spinetoram
5.625
3.75
50.00%
84.91%
98.28%


8
Novaluron + Spinetoram
5.625
1.875
55.26%
81.13%
87.93%


9
Novaluron + Spinetoram
3.75
7.5
65.79%
83.02%
94.83%


10
Novaluron + Spinetoram
3.75
5.625
50.00%
81.13%
89.66%


11
Novaluron + Spinetoram
3.75
3.75
47.37%
84.91%
84.48%


12
Novaluron + Spinetoram
3.75
1.875
42.11%
71.70%
70.69%


13
Novaluron + Spinetoram
1.875
7.5
44.74%
73.58%
75.86%


14
Novaluron + Spinetoram
1.875
5.625
47.37%
69.81%
75.86%


15
Novaluron + Spinetoram
1.875
3.75
34.21%
62.26%
56.90%


16
Novaluron + Spinetoram
1.875
1.875
31.58%
49.06%
53.45%


17
Novaluron
7.5
0
55.26%
71.70%
82.76%


18
Spinetoram
0
7.5
44.74%
71.70%
84.48%


19
Novaluron
5.625
0
42.11%
64.15%
70.69%


20
Spinetoram
0
5.625
55.26%
71.70%
82.76%


21
Novaluron
3.75
0
26.32%
58.49%
62.07%


22
Spinetoram
0
3.75
31.58%
64.15%
72.41%


23
Novaluron
1.875
0
28.95%
49.06%
60.34%


24
Spinetoram
0
1.875
15.79%
45.28%
65.52%


25
Untreated controls (UTC)
0
0
0.00%
0.00%
0.00%









Example 3
Foliar applications of Pyriproxyfen and Spinetoram on Whitefly (Trialeurodes vaporariorum) Control in Peppers (Capsicum annuum)
The efficacy of Pyriproxyfen and Spinetoram on whitefly control in pepper crops was evaluated.
Two trials were conducted on pepper crops in the vegetable growing belt of La Plata (Province of Buenos Aires, Argentina) with the goal of evaluating the efficacy and interaction of foliar applications of Pyriproxyfen and Spinetoram on whiteflies. For preparation of the mixtures, Spinetoram product used is the same as in Example 1, Pyriproxyfen (EPINGLE® 1.00 g a.i./L. EC, Sumitomo Chemical Co., Ltd.) was used. Applications were made using a backpack-type sprayer fitted with a vertical bar and a constant pressure regulator, with nozzles set at 0.30 m from one another and fitted with 8002 flat fan tips. In all cases, applications were made at a rate of 900-915 l·ha (L/ha). Eight-meter-long plots were used, each divided into four ridges (eight rows of plants). Applications were made on two ridges, while the remaining two ridges were left as buffers. Experiment design consisted in random blocks at four repetitions.
The number of adult whiteflies per leaf was assessed e.g. at days 1, 3, 5, 7, 10 and 14 post-application, whereas the number of whitefly nymphs per leaf was evaluated e.g. at 10, 14, 21 and 29 days post-application. For this assessment, ten leaves were taken from plants located in the central rows of each plot, taking care to avoid the initial and final meter of each plot. Efficacy of control (%) was calculated based on untreated control.
Description of Each Trial
















Rate of




Phenological
application



Location
Status
(l · ha−1)




















Trial 1
Jurado (Olmos)
Harvest
900 lts/ha



Trial 2
Expovictor (Florencio
Harvest
915 lts/ha




Varela)









Results











Trial 1









Efficacy of control (%)












amount of ai
amount of ai
Adults
Nymphs




















Spinetoram,
Pyriproxyfen,
1
3
5
7
10
14
10
14
21
29



g/ha
g/ha
DAA
DAA
DAA
DAA
DAA
DAA
DAA
DAA
DAA
DAA






















1
60.0
100
66.57%
78.44%
70.26%
77.57%
68.79%
81.74%
100.00% 
94.82%
100.00% 
96.08%


2
60.0
75
67.57%
78.89%
77.59%
71.21%
72.67%
78.34%
100.00% 
93.06%
100.00% 
96.85%


3
60.0
50
53.20%
66.32%
61.25%
45.42%
55.13%
66.88%
100.00% 
65.53%
86.16%
96.06%


4
60.0
25
46.51%
54.34%
52.24%
29.72%
45.56%
57.75%
93.42%
82.82%
96.49%
98.43%


5
45.0
100
61.31%
70.06%
73.82%
76.64%
60.36%
75.37%
93.42%
94.82%
100.00% 
98.43%


6
45.0
75
57.33%
67.37%
65.86%
57.38%
56.72%
59.02%
87.23%
70.70%
51.86%
98.43%


7
45.0
50
48.08%
57.93%
56.01%
49.72%
52.62%
50.96%
70.99%
62.01%
72.52%
95.28%


8
45.0
25
43.24%
52.69%
47.21%
34.21%
35.76%
39.28%
74.27%
36.23%
89.67%
90.55%


9
30.0
100
53.20%
67.66%
66.28%
77.57%
61.05%
67.52%
74.08%
82.71%
96.49%
98.43%


10
30.0
75
48.65%
64.52%
61.25%
70.84%
46.01%
58.17%
90.33%
58.59%
89.67%
59.06%


11
30.0
50
40.97%
48.35%
43.65%
48.04%
33.49%
56.05%
80.66%
62.11%
62.19%
96.06%


12
30.0
25
29.59%
42.81%
41.14%
31.96%
26.42%
42.04%
83.75%
43.06%
72.52%
88.98%


13
15.0
100
53.49%
64.67%
63.55%
82.43%
56.95%
71.97%
83.75%
67.29%
86.36%
85.04%


14
15.0
75
40.54%
42.07%
51.40%
58.69%
41.69%
47.98%
77.37%
48.34%
−10.33% 
59.85%


15
15.0
50
36.98%
41.92%
34.44%
45.61%
31.44%
39.28%
83.95%
62.01%
89.67%
85.04%


16
15.0
25
33.85%
39.97%
33.39%
23.36%
21.64%
31.85%
83.75%
63.77%
82.85%
93.70%


17
60.0
0
32.15%
44.76%
54.96%
55.14%
35.54%
42.68%
54.74%
51.66%
37.40%
74.79%


18
0.0
100
43.53%
63.02%
63.55%
69.35%
56.95%
45.65%
67.89%
51.76%
69.01%
88.19%


19
15.0
0
10.10%
15.27%
 0.71%
 9.16%
−16.17% 
−14.65% 
61.32%
 8.59%
−3.31%
61.41%


20
0.0
25
19.20%
30.09%
25.01%
36.64%
18.91%
 9.55%
54.74%
24.12%
58.68%
73.25%


21
45.0
0
20.34%
29.19%
32.76%
31.96%
23.46%
12.95%
45.26%
41.30%
30.99%
74.00%


22
0.0
75
19.91%
55.84%
47.84%
64.49%
45.56%
35.46%
48.55%
46.48%
62.19%
87.40%


23
30.0
0
34.00%
38.62%
 8.67%
37.57%
21.64%
 5.94%
74.08%
17.18%
10.74%
78.73%


24
0.0
50
34.00%
55.69%
39.04%
57.01%
33.71%
29.51%
80.85%
51.66%
38.02%
90.57%


25
0.0
0
   0%
   0%
   0%
   0%
   0%
   0%
   0%
   0%
   0%
   0%



















Trial 2









Efficacy of control (%)












a.i.
a.i.
Adults
Nymphs


















Spina
Pyrb
1
3
6
8
11
17
22
30



g/ha
g/ha
DAA
DAA
DAA
DAA
DAA
DAA
DAA
DAA




















1
60.0
100
67.01%
80.58%
64.82%
74.78%
97.02%
97.28%
83.39%
97.51%


2
60.0
75
52.82%
69.70%
58.33%
61.64%
85.09%
81.85%
68.94%
82.61%


3
60.0
50
50.00%
66.03%
50.89%
53.22%
41.74%
61.14%
35.24%
73.50%


4
60.0
25
32.76%
54.87%
39.52%
38.83%
68.58%
71.20%
29.87%
61.47%


5
45.0
100
66.72%
66.10%
67.44%
61.42%
86.24%
82.26%
66.07%
82.73%


6
45.0
75
57.13%
62.36%
53.75%
58.51%
78.67%
58.50%
61.41%
78.80%


7
45.0
50
54.61%
54.45%
52.20%
40.66%
64.22%
64.19%
55.91%
72.88%


8
45.0
25
42.42%
40.54%
39.58%
36.45%
66.28%
66.42%
−0.48%
68.77%


9
30.0
100
66.86%
65.96%
66.13%
68.84%
80.28%
72.36%
63.68%
70.64%


10
30.0
75
64.49%
62.99%
57.26%
60.67%
84.17%
70.96%
58.78%
72.26%


11
30.0
50
50.00%
48.02%
44.46%
52.18%
57.11%
58.83%
30.47%
49.00%


12
30.0
25
38.48%
40.32%
42.32%
42.25%
63.53%
43.32%
18.04%
63.97%


13
15.0
100
63.74%
65.18%
65.24%
66.39%
78.90%
64.52%
62.84%
72.94%


14
15.0
75
53.49%
51.98%
49.76%
50.27%
69.50%
60.15%
57.59%
51.00%


15
15.0
50
48.29%
58.19%
45.54%
42.25%
57.11%
39.69%
46.36%
58.85%


16
15.0
25
37.59%
41.03%
33.15%
29.11%
56.65%
48.35%
14.81%
27.56%


17
60.0
0
52.60%
53.18%
42.02%
55.42%
64.22%
61.72%
36.80%
56.92%


18
0.0
100
67.01%
64.97%
64.58%
59.70%
85.32%
70.21%
57.23%
76.43%


19
15.0
0
13.97%
 7.63%
10.18%
13.35%
12.16%
27.81%
−17.32% 
18.89%


20
0.0
25
28.90%
10.24%
15.24%
30.51%
60.09%
37.21%
14.93%
33.29%


21
45.0
0
37.74%
35.24%
24.40%
45.56%
50.00%
39.36%
19.95%
36.10%


22
0.0
75
46.14%
48.23%
33.93%
44.04%
69.27%
47.44%
58.30%
58.98%


23
30.0
0
33.51%
19.77%
 9.05%
32.24%
44.72%
 9.90%
−0.72%
45.01%


24
0.0
50
43.39%
31.00%
33.27%
41.17%
61.70%
48.84%
30.11%
41.90%


25
0.0
0
   0%
   0%
   0%
   0%
   0%
   0%
   0%
   0%





aSpinetoram;


bPyriproxyfen






Example 4
Foliar Applications of Pyriproxyfen and Spinetoram on Western Flower Thrips (Frankliniella occidentalis) in Peppers (Capsicum annuum)
The study was conducted to assess the efficacy of and interaction between Pyriproxyfen and Spinetoram on Western flower thrips in pepper crops.
Two trials were conducted on pepper crops in the vegetable growing belt of La Plata (Province of Buenos Aires, Argentina) with the goal of evaluating the efficacy and interaction of foliar applications of Pyriproxyfen and Spinetoram on Western flower thrips. For preparation of the mixtures, the products used are the same as in Example 3.
Applications were made using a backpack-type sprayer fitted with a vertical bar and a constant pressure regulator, with nozzles set at 0.30 m from one another and fitted with 8002 flat fan tips. In all cases, applications were made at a rate of 790-937 l·ha−1. Eight-meter-long plots were used, each divided into four ridges (eight rows of plants). Applications were made on two ridges, while the remaining two ridges were left as buffers. Experiment design consisted in random blocks at four repetitions.
The number of thrips (all stages) per flower was assessed at days e.g. 1, 3, 5, 7, 10 and 14 post-application. For this assessment, ten flowers were taken from plants located in the central rows of each plot, taking care to avoid the initial and final meter of each plot. Efficacy of control (%) was calculated based on untreated control.
Detailed Description of Each Trial:
















Rate of




Phenological
application



Location
Status
(l · ha−1)







Trial 1
Jurado (Olmos)
Harvest
937 lts/ha


Trial 2
Expovictor (Florencio Varela)
Harvest
790 lts/ha



















Trial 1











amount of ai
amount of ai
Efficacy of control (%)
















Spinetoram,
Pyriproxyfen,
1
3
5
7
10
14



g/ha
g/ha
DAA
DAA
DAA
DAA
DAA
DAA


















1
60.0
100
81.38%
92.21%
95.94%
89.42%
88.59%
77.33%


2
60.0
75
67.02%
80.33%
83.25%
80.77%
78.52%
72.44%


3
60.0
50
62.23%
71.72%
82.74%
75.96%
79.19%
61.33%


4
60.0
25
48.94%
65.16%
76.65%
68.27%
65.77%
53.33%


5
45.0
100
69.15%
71.31%
90.36%
81.73%
83.89%
63.11%


6
45.0
75
43.62%
69.26%
85.28%
73.08%
72.48%
49.33%


7
45.0
50
36.17%
59.02%
78.68%
72.12%
70.47%
42.53%


8
45.0
25
39.89%
59.02%
76.14%
64.42%
59.06%
21.78%


9
30.0
100
73.40%
71.31%
87.31%
64.42%
72.48%
49.24%


10
30.0
75
50.53%
59.02%
71.07%
56.73%
53.69%
36.44%


11
30.0
50
38.30%
48.36%
72.59%
51.92%
55.03%
41.33%


12
30.0
25
35.64%
39.75%
60.91%
37.50%
42.95%
24.44%


13
15.0
100
52.13%
70.12%
80.71%
59.62%
69.13%
47.56%


14
15.0
75
45.21%
47.54%
80.20%
48.08%
54.36%
38.22%


15
15.0
50
23.94%
40.98%
71.57%
40.38%
50.34%
36.89%


16
15.0
25
21.81%
41.39%
62.44%
32.69%
48.32%
28.44%


17
60.0
0
43.62%
70.90%
83.25%
61.54%
65.10%
47.56%


18
0.0
100
22.34%
64.34%
81.73%
57.69%
65.77%
36.44%


19
15.0
0
 6.38%
30.33%
41.12%
 1.92%
23.49%
23.11%


20
0.0
25
12.77%
28.69%
61.42%
33.65%
34.23%
29.33%


21
45.0
0
30.85%
45.49%
59.39%
53.85%
63.76%
22.22%


22
0.0
75
27.66%
49.18%
63.45%
50.00%
65.77%
42.67%


23
30.0
0
17.55%
41.80%
42.13%
18.27%
32.21%
25.78%


24
0.0
50
40.43%
43.03%
48.73%
47.12%
40.27%
45.78%


25
0.0
0
   0%
   0%
   0%
   0%
   0%
   0%



















Trial 2











mount of ai
amount of ai
Efficacy of control (%)














Spinetoram
Pyriproxyfen
1
3
6
8



g/ha
g/ha
DAA
DAA
DAA
DAA

















1
Pyriproxyfen + Spinetoram
60.0
100
73.11%
91.49%
75.10%
72.53%


2
Pyriproxyfen + Spinetoram
60.0
75
61.86%
85.49%
69.22%
67.79%


3
Pyriproxyfen + Spinetoram
60.0
50
58.68%
76.40%
57.16%
52.77%


4
Pyriproxyfen + Spinetoram
60.0
25
50.12%
67.12%
54.90%
55.73%


5
Pyriproxyfen + Spinetoram
45.0
100
78.48%
78.53%
68.14%
63.44%


6
Pyriproxyfen + Spinetoram
45.0
75
57.70%
70.60%
61.76%
54.35%


7
Pyriproxyfen + Spinetoram
45.0
50
60.15%
62.67%
52.35%
53.56%


8
Pyriproxyfen + Spinetoram
45.0
25
53.30%
60.74%
54.61%
52.96%


9
Pyriproxyfen + Spinetoram
30.0
100
64.06%
73.69%
65.69%
70.16%


10
Pyriproxyfen + Spinetoram
30.0
75
61.12%
61.70%
63.24%
56.72%


11
Pyriproxyfen + Spinetoram
30.0
50
45.97%
55.71%
51.67%
50.20%


12
Pyriproxyfen + Spinetoram
30.0
25
46.45%
50.48%
50.10%
55.73%


13
Pyriproxyfen + Spinetoram
15.0
100
66.75%
64.60%
64.61%
64.23%


14
Pyriproxyfen + Spinetoram
15.0
75
64.30%
62.67%
48.63%
52.96%


15
Pyriproxyfen + Spinetoram
15.0
50
55.01%
47.97%
47.45%
53.95%


16
Pyriproxyfen + Spinetoram
15.0
25
37.65%
52.22%
42.35%
44.66%


17
Spinetoram
60.0
0
55.99%
61.70%
70.88%
62.06%


18
Pyriproxyfen
0.0
100
31.30%
47.20%
43.14%
44.86%


19
Spinetoram
15.0
0
30.56%
28.63%
33.04%
16.01%


20
Pyriproxyfen
0.0
25
16.14%
17.79%
19.22%
−14.82% 


21
Spinetoram
45.0
0
38.14%
43.91%
55.10%
50.40%


22
Pyriproxyfen
0.0
75
35.45%
32.11%
16.08%
32.61%


23
Spinetoram
30.0
0
37.90%
39.85%
39.12%
33.20%


24
Pyriproxyfen
0.0
50
35.70%
22.05%
28.92%
37.55%


25
Untreated controls (UTC)
0
0
   0%
   0%
   0%
   0%









Example 5
Foliar Applications of Tau-Fluvalinate and Spinetoram on Whiteflies (Trialeurodes vaporariorum) in Peppers (Capsicum annuum)
Two trials were conducted on pepper crops in the vegetable growing belt of La Plata (Province of Buenos Aires, Argentina) with the goal of evaluating the efficacy and interaction of foliar applications of Tau-fluvalinate and Spinetoram in whitefly control. For preparation of the mixtures, Spinetoram product used was the same as in Example 1, Tau-fluvalinate (Mavrik® 240 g a.i./L EW, Makhteshim Agan Group Company) was used.
Applications were made using a backpack-type sprayer fitted with a vertical bar and a constant pressure regulator, with nozzles set at 0.30 in from one another and fitted with 8002 flat fan tips. In all cases, applications were made at a rate of 810-900 l·ha−1. Eight-meter-long plots were used, each divided into four ridges (eight rows of plants). Applications were made on two ridges, while the remaining two ridges were left as buffers. Experiment design consisted in random blocks at four repetitions.
The number of adult whiteflies per leaf was assessed at days e.g. 1, 3, 5, 7, 10 and 14 post-application, whereas the number of whitefly nymphs per leaf was evaluated at e.g. 11, 15 and 21 days post-application. For this assessment, ten leaves were taken from plants located in the central rows of each plot, taking care to avoid the initial and final meter of each plot. Efficacy of Control (%) was calculated based on untreated control.







TABLE A







Detailed description of each trial













Rate of




Phenological
application



Location
Status
(l/ha)





Trial 1
Serrano (Olmos)
Harvest
900 lts/ha


Trial 2
Expovictor (Florencio
Harvest
810 lts/ha



Varela)









Results











Trial 1









Efficacy of control (%)












amount of ai
amount of ai
Adults
Nymphs



















Spinetoram
Tau-fluvalinate
1
3
5
7
10
14
11
15
21



g/ha
g/ha
DAA
DAA
DAA
DAA
DAA
DAA
DAA
DAA
DAA





















1
60.0
30
69.60%
83.64%
76.85%
80.28%
81.09%
65.31%
94.44%
95.28%
71.88%


2
60.0
22.5
61.85%
71.78%
70.85%
68.71%
69.83%
64.25%
76.03%
85.32%
56.05%


3
60.0
15
32.50%
57.10%
51.81%
60.14%
62.73%
60.07%
68.71%
79.87%
64.04%


4
60.0
7.5
−4.78%
41.40%
41.21%
57.89%
61.34%
60.47%
61.41%
78.44%
44.62%


5
45.0
30
66.06%
82.20%
70.08%
72.56%
74.90%
72.75%
78.36%
86.45%
84.08%


6
45.0
22.5
48.09%
70.52%
44.00%
64.92%
68.78%
64.54%
75.44%
55.03%
33.07%


7
45.0
15
7.07%
57.28%
25.52%
49.32%
56.23%
51.44%
65.20%
70.02%
43.19%


8
45.0
7.5
−19.60%
44.16%
13.39%
20.66%
27.84%
36.15%
51.75%
66.53%
26.12%


9
30.0
30
61.85%
74.00%
63.46%
70.63%
74.84%
69.17%
64.04%
87.16%
68.72%


10
30.0
22.5
37.76%
59.20%
46.51%
59.72%
67.25%
66.21%
64.62%
58.62%
55.25%


11
30.0
15
18.07%
32.53%
25.66%
46.42%
57.12%
58.44%
54.39%
68.58%
47.09%


12
30.0
7.5
−1.53%
20.49%
−3.84%
28.34%
41.94%
42.86%
30.12%
56.98%
31.14%


13
15.0
30
65.11%
73.64%
59.83%
68.06%
74.09%
67.14%
61.12%
81.83%
79.26%


14
15.0
22.5
41.30%
55.78%
39.26%
56.25%
65.56%
61.41%
45.91%
69.94%
42.17%


15
15.0
15
4.88%
33.25%
20.01%
37.66%
49.83%
53.44%
51.75%
54.43%
41.59%


16
15.0
7.5
−31.17%
14.44%
−3.07%
16.02%
30.87%
38.43%
42.11%
39.02%
18.84%


17
60.0
0
32.31%
45.66%
34.59%
44.96%
45.66%
53.07%
59.36%
83.57%
56.61%


18
0.0
30
44.84%
64.29%
53.84%
57.00%
62.38%
68.81%
68.42%
74.84%
67.17%


19
15.0
0
4.11%
16.00%
5.23%
29.51%
25.81%
45.30%
40.94%
55.44%
40.58%


20
0.0
7.5
14.05%
39.78%
23.92%
46.84%
52.61%
49.98%
40.64%
62.01%
40.92%


21
45.0
0
14.63%
37.09%
20.15%
40.89%
43.33%
49.90%
41.82%
67.76%
48.99%


22
0.0
22.5
21.32%
49.07%
30.61%
51.52%
62.53%
58.85%
38.30%
69.40%
62.67%


23
30.0
0
−3.25%
21.51%
0.56%
22.62%
31.91%
41.76%
21.92%
54.41%
41.03%


24
0.0
15
14.53%
40.86%
20.78%
39.63%
52.90%
49.00%
47.66%
64.27%
46.97%



















Trial 2









Efficacy of control (%)












amount of ai
amount of ai
Adults
Nymphs

















Spinetoram
Tau-fluvalinate
1
3
6
10
14
21
28



g/ha
g/ha
DAA
DAA
DAA
DAA
DAA
DAA
DAA



















1
60.0
72.0
4.38%
72.91%
90.30%
78.32%
76.47%
84.45%
75.95%


2
60.0
54.0
2.92%
58.88%
82.49%
61.70%
46.04%
79.08%
62.78%


3
60.0
36.0
−5.22% 
41.51%
73.74%
51.67%
41.43%
73.02%
58.69%


4
60.0
18.0
7.72%
29.05%
55.35%
38.96%
50.12%
58.36%
37.58%


5
45.0
72.0
4.80%
74.39%
76.57%
69.76%
70.85%
83.85%
65.74%


6
45.0
54.0
−0.42% 
64.47%
72.19%
60.07%
59.60%
71.30%
44.21%


7
45.0
36.0
4.18%
35.82%
56.03%
34.96%
58.57%
62.08%
31.68%


8
45.0
18.0
4.80%
26.99%
54.81%
36.46%
71.86%
37.14%
 6.31%


9
30.0
72.0
10.86% 
77.13%
80.40%
70.01%
74.70%
73.15%
55.50%


10
30.0
54.0
1.25%
55.35%
66.33%
56.89%
71.61%
60.92%
48.25%


11
30.0
36.0
−3.13% 
50.44%
62.56%
50.77%
58.30%
43.71%
26.24%


12
30.0
18.0
5.64%
49.95%
67.14%
53.14%
66.23%
40.41%
16.48%


13
15.0
72.0
1.46%
81.26%
81.82%
74.98%
58.82%
62.72%
49.16%


14
15.0
54.0
9.81%
63.40%
76.23%
59.58%
57.54%
59.32%
34.62%


15
15.0
36.0
12.11% 
49.66%
62.22%
46.70%
54.48%
37.65%
10.62%


16
15.0
18.0
−1.67% 
46.91%
55.29%
44.01%
50.13%
39.78%
 7.89%


17
60.0
0.0
7.31%
39.74%
54.07%
41.73%
51.92%
46.70%
27.36%


18
0.0
72.0
11.06% 
58.88%
71.45%
59.74%
71.10%
73.66%
59.37%


19
15.0
0.0
−6.26% 
−19.92% 
18.92%
−1.87%
37.60%
22.78%
−21.39% 


20
0.0
18.0
3.55%
18.25%
34.61%
 7.74%
54.73%
37.55%
−17.56% 


21
45.0
0.0
−15.45% 
31.01%
52.19%
29.83%
57.03%
41.27%
 6.48%


22
0.0
54.0
−15.03% 
56.82%
64.44%
43.60%
62.67%
58.68%
11.29%


23
30.0
0.0
−14.20% 
12.07%
25.32%
18.91%
49.37%
32.44%
−15.76% 


24
0.0
36.0
1.67%
22.96%
48.35%
10.27%
67.97%
50.50%
 1.95%


25
0.0
0
  0%
   0%
   0%
   0%
   0%
   0%
   0%









Example 6
Foliar Applications of Tau-Fluvalinate and Spinetoram on Western Flower Thrips (Frankliniella occidentalis) in Peppers (Capsicum annuum)
The study was conducted to assess the efficacy of and interaction between Tau-fluvalinate and Spinetoram on Western flower thrips in pepper crops.
Two trials were conducted on pepper crops in the vegetable growing belt of La Plata (Province of Buenos Aires, Argentina) with the goal of evaluating the efficacy and interaction of foliar applications of Tau-fluvalinate and Spinetoram on Western flower thrips. 
Applications were made using a backpack-type sprayer fitted with a vertical bar and a constant pressure regulator, with nozzles set at 0.30 m from one another and fitted with 8002 flat fan tips. In all cases, applications were made at a rate of 790-937 l·ha−1. Eight-meter-long plots were used, each divided into four ridges (eight rows of plants). Applications were made on two ridges, while the remaining two ridges were left as buffers. Experiment design consisted in random blocks at four repetitions.
The number of thrips (all stages) per flower was assessed at days e.g. 1, 3, 5, 7, 10 and 14 post-application. For this assessment, ten flowers were taken from plants located in the central rows of each plot, taking care to avoid the initial and final meter of each plot. Efficacy of Control (%) was calculated based on untreated control.
Description of Each Trial
















Rate of





application



Location
Phenological Status
(l · ha)







Trial 1
Serrano (Olmos)
Harvest
937 lts/ha


Trial 2
Expovictor (Florencio
Harvest
790 lts/ha



Varela)



















Trial 1











amount of ai
amount of ai
Efficacy of control (%)
















Spinetoram,
Tau-fluvalinate,
1
3
5
7
10
14



g/ha
g/ha
DAA
DAA
DAA
DAA
DAA
DAA


















1
60.0
72.0
44.33%
86.49%
97.67%
93.91%
90.51%
81.96%


2
60.0
54.0
39.63%
82.14%
87.67%
87.54%
80.09%
76.26%


3
60.0
36.0
27.57%
75.16%
80.00%
79.42%
78.47%
63.24%


4
60.0
18.0
35.61%
64.05%
70.47%
67.83%
73.15%
53.42%


5
45.0
72.0
23.88%
77.78%
86.74%
76.81%
83.80%
77.40%


6
45.0
54.0
39.30%
69.50%
73.72%
74.78%
74.07%
68.49%


7
45.0
36.0
20.52%
59.91%
60.93%
64.35%
69.21%
56.85%


8
45.0
18.0
27.57%
54.90%
55.12%
51.30%
65.74%
48.63%


9
30.0
72.0
39.64%
68.85%
77.91%
81.16%
82.41%
74.20%


10
30.0
54.0
43.00%
60.57%
66.98%
68.99%
71.99%
65.30%


11
30.0
36.0
19.18%
48.80%
58.37%
58.26%
65.28%
55.02%


12
30.0
18.0
38.30%
35.95%
49.77%
42.90%
57.18%
45.89%


13
15.0
72.0
52.72%
71.46%
75.81%
66.67%
72.92%
63.24%


14
15.0
54.0
49.36%
61.87%
65.12%
55.36%
64.81%
55.71%


15
15.0
36.0
43.33%
54.03%
50.47%
57.10%
57.87%
39.04%


16
15.0
18.0
24.14%
45.53%
35.81%
42.32%
53.47%
38.81%


17
60.0
0.0
68.61%
72.98%
66.28%
54.78%
56.25%
48.17%


18
0.0
72.0
67.81%
66.01%
68.84%
60.87%
64.81%
36.76%


19
15.0
0.0
51.31%
57.52%
53.49%
40.87%
40.05%
36.30%


20
0.0
18.0
36.42%
29.41%
40.23%
25.22%
39.81%
38.58%


21
45.0
0.0
53.92%
59.69%
58.84%
43.48%
46.30%
34.25%


22
0.0
54.0
56.54%
62.53%
59.30%
46.38%
51.39%
37.21%


23
30.0
0.0
51.71%
51.42%
50.47%
37.68%
42.82%
28.77%


24
0.0
36.0
46.28%
55.34%
55.35%
44.64%
42.13%
26.94%



















Trial 2











amount of ai
amount of ai
Efficacy of control (%)















Spinetoram
Tau-fluvalinate
1
3
5
10
16



g/ha
g/ha
DAA
DAA
DAA
DAA
DAA

















1
60.0
72.0
95.81%
96.26%
93.61%
96.23%
42.74%


2
60.0
54.0
90.35%
89.69%
89.23%
91.21%
43.79%


3
60.0
36.0
74.85%
79.28%
73.49%
79.39%
32.88%


4
60.0
18.0
55.65%
51.00%
53.73%
64.33%
27.64%


5
45.0
72.0
95.13%
92.30%
94.79%
94.87%
41.90%


6
45.0
54.0
79.14%
81.09%
78.22%
82.11%
40.64%


7
45.0
36.0
62.38%
66.14%
60.83%
67.57%
37.08%


8
45.0
18.0
46.00%
49.04%
46.51%
53.56%
28.27%


9
30.0
72.0
79.59%
80.86%
79.64%
80.65%
38.76%


10
30.0
54.0
73.20%
77.69%
74.44%
79.71%
38.13%


11
30.0
36.0
48.15%
52.32%
53.73%
61.19%
33.30%


12
30.0
18.0
42.20%
44.17%
48.40%
53.45%
30.16%


13
15.0
72.0
66.28%
65.23%
61.42%
59.73%
35.19%


14
15.0
54.0
61.70%
60.36%
52.66%
57.11%
30.58%


15
15.0
36.0
46.59%
50.06%
40.36%
44.04%
41.06%


16
15.0
18.0
30.12%
31.60%
28.28%
33.58%
30.37%


17
60.0
0.0
71.25%
73.84%
72.66%
77.41%
35.61%


18
0.0
72.0
43.18%
53.34%
51.48%
55.86%
33.93%


19
15.0
0.0
23.20%
38.17%
37.16%
42.05%
41.06%


20
0.0
18.0
−0.88%
20.16%
14.44%
22.70%
23.44%


21
45.0
0.0
60.82%
57.87%
62.72%
66.84%
29.11%


22
0.0
54.0
31.58%
43.37%
45.56%
48.54%
30.37%


23
30.0
0.0
51.27%
46.21%
53.49%
56.80%
26.38%


24
0.0
36.0
19.59%
39.75%
32.43%
38.91%
33.72%









Phytotoxicity

No symptoms were observed in any of the treatments of Examples 1-6.
CONCLUSIONS
The results show very good efficacy control over the pests with a combination of spinetoram and the co-component (Novaluron, Pyriproxyfen, Tau-fluvalinate). Additionally these combinations, afford very good control over more than one pest that tend to be present on the plant at the same time.
While this invention has been shown and described with reference to preferred embodiments thereof, it will be understood by those skilled in the art that many alternatives, modifications and variations may be made thereto without departing from the spirit and scope of the invention. Accordingly, it is intended to embrace all such alternatives, modifications, and variations that fall within the spirit and broad scope of the appended claims.
All publications, patents and patent applications mentioned in this specification are herein incorporated in their entirety by reference into the specification, to the same extent as if each individual publication, patent or patent application was specifically and individually indicated to be incorporated herein by reference.

## Claims

1. A pesticidal mixture comprising:
(a) spinetoram; and
(b) a compound of group A selected from novaluron, pyriproxyfen, tau-fluvalinate, and a combination thereof,
wherein a weight ratio of spinetoram to the compound of group A is from 1:10 to 10:1.

2. The pesticidal mixture according to claim 1 in a synergistic effective amount.

3. A pesticidal composition comprising the pesticidal mixture according to claim 1; and an agriculturally acceptable carrier.

4. The pesticidal composition according to claim 3 for use in controlling pests in a crop or a locus thereof.

5. A method for controlling pests in a crop, comprising:
applying a pesticidal combination to pests or a locus, where control of the pest is desired,
wherein the pesticidal combination comprises:
(a) spinetoram; and
(b) a compound of group A selected from novaluron, pyriproxyfen, tau-fluvalinate, and combination thereof, and
wherein a weight ratio of spinetoram to the compound of group A is from 1:10 to 10:1.

6. The method according to claim 5, wherein an application rate of spinetoram is from 1 g/ha to 500 g/ha.

7. The method according to claim 5 , wherein an application rate of the compound of group A is from 1 g/ha to 500 g/ha.

8. The method according to claim 5, wherein an application rate of the pesticidal combination according to claim 5 is from 1 g/ha to 1000 g/ha.

9. The method according to claim 5, wherein spinetoram and the compound of group A are applied concomitantly or sequentially.

10. A kit comprising (a) at least one container including spinetoram; (b) at least one container including a compound of group A selected from novaluron, pyriproxyfen, tau-fluvalinate, and a combination thereof; and (c) instructions for applying a combination of said spinetoram and said compound of group A a pest or a locus, where control of the pest is desired, wherein a weight ratio of spinetoram to the compound of group A is from 1:10 to 10:1.

11. The kit of claim 10 wherein said spinetoram and said compound of group A are applied concomitantly or sequentially.

12. A kit comprising
(a) at least one container including (i) spinetoram; and (ii) a compound of group A selected from novaluron, pyriproxyfen, tau-fluvalinate, and a combination thereof; and
(b) instructions for applying said spinetoram and said compound of group A onto a pest or a locus, where control of the pest is desired, wherein a weight ratio of spinetoram to the compound of group A is from 1:10 to 10:1.