# Patent

## Title
Nucleic acid molecules encoding enzymes that confer disease resistance in jute

## Abstract

The invention further relates to vectors, host cells, seeds, and plants comprising such a nucleic acid molecule. One aspect of the invention is an isolated antibody or antigen binding fragment thereof that specifically binds to a polypeptide molecule of the present invention. One aspect of the invention is a plane or plant cell transfected by a vector of the present invention. One aspect of the invention relates to isolated nucleic acid molecules and fragments thereof encoding enzymes or proteins involved in disease resistance in jute.

## Background
RELATED APPLICATIONS
This application is a National Stage application of PCT/US12/041467 filed Jun. 8, 2012, which claims the benefit of priority to U.S. Provisional Patent Application Ser. No. 61/500,407, filed Jun. 23, 2011.


FIELD OF THE INVENTION
One aspect of the invention relates to polynucleotides that encode polypeptides that confer disease resistance in jute. The invention further relates to vectors and bacteria containing such a nucleic acid molecule, as well as plant cells and plants transformed with at least one of the nucleic acid molecules.
BACKGROUND OF THE INVENTION
Unlike humans, plants do not have an immune system, but at different stages of evolution they have developed a wide array of defense mechanisms in order to withstand pathogenic challenges. A well-characterized mechanism of plant defense is mediated by interaction between a plant's disease-resistance proteins, encoded by disease-resistance (R) genes, and the corresponding avirulence (Avr) genes expressed by the pathogen. (Bent, A F and Mackey, D, 2007, Elicitors, Evectors, and R-genes: the new paradigm and a lifetime supply of questions, Annu Rev Phytopathol, 45:399-436.). This interaction leads to activation of the plant's response, which in turn results in increased resistance to disease. The putative ligand-receptor complex initiates a series of signal transduction cascades contributing to disease resistance. (Baker, B et al., 1997, Signaling in plant-microbe interactions, Science, 273:726-733). Some cellular events that characterize resistance include oxidative burst, induction of defense gene expression, and rapid cell death at the site of infection. (Dhalowal, H S and Uchimiya, H, 1999, Genetic engineering for disease and pest resistance in plants, Plant Biotechnol, 16:255-261).
There are various types of disease-resistance genes. During different evolutionary events, plants have gained and omitted different disease-resistance genes. Therefore, a plant that displays resistance against a certain pathogen may have the gene(s) necessary for resistance against that pathogen. Consequently, identification of disease-resistant genes is a promising field of plant molecular biotechnology.
Disease resistance (R) genes, which interact with the Avr genes of different pathogens, have been isolated from numerous plant species. These genes confer resistance to a wide range of plant pathogens, including bacteria, fungi, oomycetes, viruses, and nematodes. (Baker et al., 1997; Bent, 1996; Hammond-Kosack, K E and Jones, J D G, 1997, Plant disease resistance genes, Annual Review of Plant Physiology and Plant Molecular Biology, 48:573-605; Ellis, C N et al., 1988, Topical Tretinoin for Photoaged Skin-Reply, JAMA, 259(22):3274-78). Although the products of avirulence genes typically have little homology with each other, the reported R genes have conserved functional domains, such as the protein kinase (PK) domain, Leucine-rich regions (LRR), and nucleotide-binding sites (NBS). (Bent, 1996; Ellis et al., 1988).
Based on the structure of R gene products, the R genes can be classified into four main classes: Nucleotide-Binding Site-Leucine-Rich Repeat (NBS-LRR); Leucine-Rich Repeat-Trans-Membrane domain-Protein Kinase (LRR-TM-PK); Leucine-Rich Repeat-Trans-Membrane domain (LRR-TM); and Protein Kinase (PK). (Bent, 1996; Ellis et al., 1988; Hammond-Kosack and Jones, 1997). The largest number of characterized R proteins is of the NBS-LRR type. These NBS-LRR proteins can be further subdivided into two sub-families based on the presence or absence of a region homologous to the toll and interleukin-1 receptor (TIR) domain at their N-terminus. (Baker et al., 1997; Parker, M F et al., 1997, Molecular characterization of adenocarcinoma of the cervix, Gynecol Oncol., 64(2):242-51). The two sub-families are designated TIR and non-TIR, respectively. Most members of the non-TIR subfamily encode a putative coiled-coil (CC) domain at their N-terminus. (Pan, P et al., 2000, Determination of the in situ bactericidal activity of an essential oil mouth rinse using a vital stain method, J Clin Periodontol., 27:256-261). TIR and non-TIR NBS-LRR R genes can be distinguished by amino acid motifs found within the NBS domain itself. (Meyers, B C et al., 2003, Genome-wide analysis of NBS-LRR-encoding genes in Arabidopsis, Plant Cell, 15:809-834; Pan et al., 2000). They also differ at the functional level, based on their apparent involvement in different signal transduction pathways. (Aarts, N et al., 1998, Different requirements for EDS1 and NDR1 by disease resistance genes define at least two R gene-mediated signaling pathways in Arabidopsis, Proc. Natl. Acad. Sci. USA, 95:10306-10311).
NBS sequences are abundant in plant genomes. (Meyers et al., 1999). In fact, the Arabidopsis genome contains 150 NBS-encoding genes, including 85 TIR NBS-LRR genes, 43 non-TIR NBS-LRR genes, and numerous truncated TIR or CC-NBS genes that lack an LRR domain (The Arabidopsis Genome Initiative, 2000). No precise functions have yet been established for each of these resistance gene analogs (RGAs). (Kanazin, V. et al., 1996, Resistance gene analogs are conserved and clustered in soybean, PNAS, USA, 93(21):11746-11750; Leister, D et al., 1996, PCR based approach for isolating pathogen resistance genes from potato with potential for wide application in plants, Nature Genetics, 14(4):421-429; Yu, Y G et al., 1996, Isolation of a super family of candidate disease-resistance genes in soybean based on a conserved nucleotide-binding site, PNAS, USA 93(21):11751-11756). Thus, RGAs are valuable as potential sources of active R genes.
SUMMARY OF THE INVENTION
Among other things, the present invention provides nucleic acid molecules encoding enzymes from jute which are involved in the plant's disease-resistance pathways, and from which genetically-modified plants may be produced with enhanced disease-resistance against various pathogens.
One aspect of the invention is an isolated nucleic acid molecule having at least 90% sequence identity to a nucleic acid sequence selected from the group consisting of: SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, and 27.
In one embodiment, the nucleic acid sequence is selected from the group consisting of: SEQ ID NO: 1, 3, and 5.
In one embodiment, the nucleic acid sequence is selected from the group consisting of SEQ ID NO: 7.
In one embodiment, the nucleic acid sequence is selected from the group consisting of: SEQ ID NO: 9, 11, and 13.
In one embodiment, the nucleic acid sequence is selected from the group consisting of SEQ ID NO: 15.
In one embodiment, the nucleic acid sequence is selected from the group consisting of: SEQ ID NO: 17 and 19.
In one embodiment, the nucleic acid sequence is selected from the group consisting of: SEQ ID NO: 25 and 27.
In one embodiment, the nucleic acid sequence is selected from the group consisting of SEQ ID NO: 23.
One aspect of the invention is an isolated polypeptide molecule having at least 90% sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, and 28.
Another aspect of the present invention relates to a pair of primers (forward and reverse) useful for the amplification of cDNA selected from the group consisting of SEQ ID NO 29 and SEQ ID NO 30; SEQ ID NO 31 and SEQ ID NO 32; SEQ ID NO 33 and SEQ ID NO 34; SEQ ID NO 35 and SEQ ID NO 36; and SEQ ID NO 37 and SEQ ID NO 38.
In certain embodiments, the present invention relates to any one of the aforementioned isolated polynucleotide molecules or isolated polypeptide molecules, wherein said molecule has at least 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% sequence identity to any one of the sequences identified by a SEQ ID NO.
One aspect of the invention is an expression vector comprising an nucleic acid molecule of the present invention.
One aspect of the invention is an isolated antibody or antigen binding fragment thereof that specifically binds to a polypeptide molecule of the present invention.
One aspect of the invention is a plane or plant cell transfected by a vector of the present invention.
One aspect of the invention is a material derived from a transgenic plant of the present invention.
One aspect of the invention is a seed from a plant transfected by a vector of the present invention.
One aspect of the invention is a method for making a transgenic plant, comprising the steps of transfecting at least one plant cell with a vector of the present invention, and growing said at least one plant cell into a plant.
One aspect of the invention is a method of improving growth, fiber yield, fiber strength, disease resistance, or water utilization in a jute plant, comprising incorporating in to a jute plant a non-native nucleic acid sequence of the present invention.



BRIEF DESCRIPTION OF THE DRAWINGS
FIGS. 1a and 1b: Protein sequence alignment of Corchorus olitorius Toll and Interleukin-1 Receptor domain at their N-terminus Nucleotide-Binding Site-Leucine-Rich Repeat (ColTIR-NBS-LRR)1 and ColTIR-NBS-LRR2 with plant TIR-NBS-LRR protein sequences.
FIGS. 2a and 2b: Protein sequence alignment of ColTIR-NBS-LRR3 with plant TIR-NBS-LRR and other resistance-like protein sequences. FIG. 2 discloses SEQ ID NOS 6 and 41-42, respectively, in order of appearance.
FIG. 3: Protein sequence alignment of ColNBS-LRR with a plant NBS-LRR protein sequence. FIG. 3 discloses SEQ ID NOS 8 and 43, respectively, in order of appearance.
FIGS. 4a and 4b: Protein sequence alignment of ColCC-NBS-LRR1 with plant CC-NBS-LRR protein sequences. FIG. 4 discloses SEQ ID NOS 10 and 44, respectively, in order of appearance.
FIGS. 5a and 5b: Protein sequence alignment of ColCC-NBS-LRR2 and ColCC-NBS-LRR3 with plant CC-NBS-LRR protein sequences. FIG. 5 discloses SEQ ID NOS 14, 45-46, and 12, respectively, in order of appearance.
FIG. 6: Protein sequence alignment of ColNBS resistance protein with plant NBS resistance protein sequences. FIG. 6 discloses SEQ ID NOS 16 and 47, respectively, in order of appearance.
FIGS. 7a and 7b: Protein sequence alignment of ColLRR1 and ColLRR-TM-PK protein with plant Leucine-Rich Repeat containing protein sequences. FIG. 7 discloses SEQ ID NOS 20 and 48-50, respectively, in order of appearance.
FIGS. 8a and 8b: Protein sequence alignment of ColCCH type with plant CCH type protein sequences. FIG. 8 discloses SEQ ID NOS 22 and 51, respectively, in order of appearance.
FIG. 9: Protein sequence alignment of ColTIR-NBS resistance protein with plant TIR-NBS resistance proteins sequences. FIG. 9 discloses SEQ ID NOS 24 and 52-53, respectively, in order of appearance.
FIGS. 10a and 10b: Protein sequence alignment of ColRGA1 and ColRGA2 with plant RGA protein sequences. FIG. 10 discloses SEQ ID NOS 28, 54, 26, and 55, respectively, in order of appearance.
FIG. 11: DNA gel of TIR-NBS-LRR resistance protein.
FIG. 12: DNA gel of TIR-NBS-LRR resistance protein.
FIG. 13: DNA gel of CC-NBS-LRR1 resistance protein.
FIG. 14: DNA gel of CC-NBS-LRR3 resistance protein.
FIG. 15: DNA gel of resistance gene analog (RGA1).



DETAILED DESCRIPTION OF THE INVENTION
Identification of the NBS-LRR Genes
The identification the NBS encoding genes in Corchorus olitorius was done using BLAST (Altschul, S F et al., 1990, Basic local alignment search tool. J Mol Biol, 215:403-410) with the in-house NBS disease resistance gene database with a high stringency e-value cutoff of 1e-25. Gene prediction was done with Augustus, and further validation of the sequences was completed using the InterProScan domain prediction program. Selection of the models was based on completeness and homology to a select set of NBS-encoding genes of other plants.
Classification of the Identified Genes
The identified genes were then classified based on their N-terminal regions (Ming et al., 2008). The TIR domain was detected by Pfam HMM searches using a model for TIR (PF01582). (Finn, R D et al., 2008, The Pfam protein families database, Nucleic Acids Res, 36:281-288) The Coiled Coil (CC) domains were identified by the COILS program using a threshold of 0.9. (Lupas, A et al., 1991, Predicting coiled coils from protein sequences, Science, 252:1162-1164). For the identification of the LRR motifs, Pfam HMM searches using models for LRR-I (PF00560), LRR_2 (PF07723) and LRR_3 (PF07725) were used. (Finn et al., 2008). Conserved motifs within the domains were identified using Multiple Expectation Maximization for Motif Elicitation. (Bailey, T L and Elkan, C, 1995, The value of prior knowledge in discovering motifs with MEME, Proc. Int. Conf. Intell. Syst. Mol. Biol., 3:2119).
Mapping with c-DNA Data:
The c-DNA sequencing of the C. olitorius genome was done by the 454 pyrosequencing platform and the identified NBS sequences were mapped to o-DNA sequences using GMAP. (Wu, T D and Watanabe, C K, 2005, GMAP: a genomic mapping and alignment program for mRNA and EST sequences, Bioinformatics, 2:1859-1875). The criteria fixed for mapping with an EST was greater than 95% sequence identity. Only the best alignment score were considered for ESTs that can be mapped to multiple locations in the genome.
An amino acid sequence alignment of putative proteins encoded by the ColTIR-NBS-LRR1 and ColTIR-NBS-LRR2 genes with other plant TIR-NBS-LRR protein sequences available in the NCBI database, using the CLUSTAL W program, is shown in FIGS. 1a and 1b. The following proteins were aligned with the putative ColTIR-NBS-LRR proteins, with the GeneBank Accession Numbers in parentheses: PtcTIR-NBS-LRR (Populus tricocarpa, gi224127726) and MtrTIR-NBS-LRR (Medicago tranculata, gi87162908).
An amino acid sequence alignment of putative proteins encoded by the ColTIR-NBS-LRR3 gene with plant TIR-NBS-LRR and other resistance-like protein sequences available in the NCBI database, using the CLUSTAL W program, is shown in FIGS. 2a and 2b. The following proteins were aligned with the putative ColTIR-NBS-LRR3 protein, with the GeneBank Accession Numbers in parentheses: PtcTIR-NBS-LRR (Populus tricocarpa, gi224126507) and Stu nematode resistance-like protein (Solunum tuberosum, gi37781360).
An amino acid sequence alignment of putative proteins encoded by the ColNBS-LRR gene with other plant NBS-LRR protein sequences available in the NCBI database, using the CLUSTAL W program, is shown in FIG. 3. The following protein was aligned with the putative ColNBS-LRR proteins, with the GeneBank Accession Numbers in parentheses: PtcNBS-LRR (Populus tricocarpa, gi224075299).
An amino acid sequence alignment of putative proteins encoded by the ColCC-NBS-LRR1 gene with other plant CC-NBS-LRR protein sequences available in the NCBI database, using the CLUSTAL W program, is shown in FIGS. 4a and 4b. The following protein was aligned with the putative ColCC-NBS-LRR protein, with the GeneBank Accession Numbers in parentheses: PtcCC-NBS-LRR (Populus tricocarpa, gi224111284).
An amino acid sequence alignment of putative proteins encoded by the ColCC-NBS-LRR2 and ColCC-NBS-LRR3 genes with other plant CC-NBS-LRR protein sequences available in the NCBI database, using the CLUSTAL W program, is shown in FIGS. 5a and 5b. The following proteins were aligned with the putative ColCC-NBS-LRR proteins, with the GeneBank Accession Numbers in parentheses: PtcCC-NBS-LRR (Populus tricocarpa, gi224059584) and PtcCC-NBS-LRR (Populus tricocarpa, gi8224132258).
An amino acid sequence alignment of putative proteins encoded by the ColNBS gene with other plant NBS protein sequences available in the NCBI database, using the CLUSTAL W program, is shown in FIG. 6. The following protein was aligned with the putative ColNBS protein, with the GeneBank Accession Numbers in parentheses: PtcNBS (Populus tricocarpa, gi224058687).
An amino acid sequence alignment of putative proteins encoded by the ColLLR1 and ColLLR-TM-PK genes with other plant LRR protein sequences available in the NCBI database, using the CLUSTAL W program, is shown in FIGS. 7a and 7b. The following proteins were aligned with the putative ColLRR proteins, with the GeneBank Accession Numbers in parentheses: AthLRR (Arabidopsis thaliana, gi42561789) and RcoLRR (Ricinus communis, gi255546155).
An amino acid sequence alignment of putative proteins encoded by the ColCCH gene with other plant CCH protein sequences available in the NCBI database, using the CLUSTAL W program, is shown in FIGS. 8a and 8b. The following protein was aligned with the putative ColCCH proteins, with the GeneBank Accession Numbers in parentheses: PtcCCH type (Ricinus communis, gi109676362).
An amino acid sequence alignment of putative proteins encoded by the ColTIR-NBS gene with other plant TIR-NBS protein sequences available in the NCBI database, using the CLUSTAL W program, is shown in FIG. 9. The following proteins were aligned with the putative ColTIR-NBS proteins, with the GeneBank Accession Numbers in parentheses: Ptc (Populus tricocarpa, gi224071947) and Rco nucleoside-triphosphatase (Ricinus communis, gi255537517).
An amino acid sequence alignment of putative proteins encoded by the ColRGA1 and ColRGA2 genes with other plant RGA protein sequences available in the NCBI database, using the CLUSTAL W program, is shown in FIGS. 10a and 10b. The following proteins were aligned with the putative ColRGA proteins, with the GeneBank Accession Numbers in parentheses: RcoRGA2 (Ricinus communis, gi255561034) and RcoRGA2 (Ricinus communis, gi255568719).
DNA gels of PCR reactions using forward and reverse primers for several jute disease-resistance enzymes are shown in FIGS. 11-15. In FIG. 11, the DNA gel is of TIR-NBS-LRR resistance protein from Corchorus olitorius. Lane 1 is the PCR product of TIR-NBS-LRR resistance protein using cDNA as a template. The forward primer and reverse primer are SEQ ID NO. 29 and 30, respectively. Lane 2 is the 1 Kb+ladder. In FIG. 12, the DNA gel is of TIR-NBS-LRR resistance protein from Corchorus olitorius. Lane 1 is the PCR product of TIR-NBS-LRR resistance protein using cDNA as a template. The forward primer and reverse primer are SEQ ID NO. 31 and 32, respectively. Lane 2 is the 1 Kb+ladder. In FIG. 13, the DNA gel is of CC-NBS-LRR1 resistance protein from Corchorus olitorius. Lane 1 is the PCR product of CC-NBS-LRR1 resistance protein using cDNA as a template. The forward primer and reverse primer are SEQ ID NO. 33 and 34, respectively. Lane 2 is the 1 Kb+ladder. In FIG. 14, the DNA gel is of CC-NBS-LRR3 resistance protein from Corchorus olitorius. Lane 1 is the PCR product of CC-NBS-LRR3 resistance protein using cDNA as a template. The forward primer and reverse primer are SEQ ID NO. 35 and 36, respectively. Lane 2 is the 1 Kb+ladder. In FIG. 15, the DNA gel is of RGA1 resistance protein from Corchorus olitorius. Lane 1 is the PCR product of RGA1 resistance protein using cDNA as a template. The forward primer and reverse primer are SEQ ID NO. 37 and 38, respectively. Lane 2 is the 1 Kb+ladder.
Definitions
A cell has been “transformed” or “transfected” by exogenous or heterologous DNA when such DNA has been introduced inside the cell. The transforming DNA may or may not be integrated (covalently linked) into the genome of the cell. In prokaryotes, yeast, and mammalian cells for example, the transforming DNA may be maintained on an episomal element such as a plasmid. With respect to eukaryotic cells, a stably transformed cell is one in which the transforming DNA has become integrated into a chromosome so that it is inherited by daughter cells through chromosome replication. The practice of the present invention contemplates a wide variety of stably transformed plant cells.
An “expression cassette” refers to a nucleic acid construct, which when introduced into a host cell, results in transcription and/or translation of a RNA and/or polypeptide, respectively. The expression cassette may include a nucleic acid comprising a promoter sequence, with or without a sequence containing mRNA polyadenylation signals, and one or more restriction enzyme sites located downstream from the promoter allowing insertion of heterologous gene sequences. The expression cassette is capable of directing the expression of a heterologous protein when the gene encoding the heterologous protein is operably linked to the promoter by insertion into one of the restriction sites. The recombinant expression cassette allows expression of the heterologous protein in a host cell when the expression cassette containing the heterologous protein is introduced into the host cell. Expression cassettes can be derived from a variety of sources depending on the host cell to be used for expression. For example, an expression cassette can contain components derived from a viral, bacterial, insect, plant, or mammalian source. In the case of both expression of transgenes and inhibition of endogenous genes (e.g., by antisense, or sense suppression) the inserted polynucleotide sequence need not be identical and can be “substantially identical” to a sequence of the gene from which it was derived. Preferably the recombinant expression cassette allows expression at an early stage of infection and/or it allows expression in substantially all cells of an organism, such as a plant. Examples of expression cassettes suitable for transformation of plants can be found in U.S. Pat. Nos. 5,880,333 and 6,002,072; International Patent Publications Nos. WO/1990/002189 and WO/2000/026388; Ainley and Key, 1990, Plant Mol. Biol., 14:949-967; and Birch, 1997, Annu. Rev. Plant Physiol. Plant Mol. Biol., 48:297-326, all of which are herein incorporated by reference.
The term “host cell” refers to a cell from any organism. Preferred host cells are derived from plants, bacteria, yeast, fungi, insects, or other animals. The term “recombinant host cell” (or simply “host cell”) refers to a cell into which a recombinant expression vector has been introduced. It should be understood that the term “host cell” is intended to refer not only to the particular subject cell but to the progeny of such a cell. Because certain modifications may occur in succeeding generations due to either mutation or environmental influences, such progeny may not, in fact, be identical to the parent cell, but are still included within the scope of the term “host cell” as used herein. Methods for introducing polynucleotide sequences into various types of host cells are well known in the art. Provided are host cells or progeny of host cells transformed with the recombinant expression cassettes of the present invention. The host cells may be plant cells. Preferably, the plant cells are jute cells.
The term “operably linked” or “operably inserted” means that the regulatory sequences necessary for expression of the coding sequence are placed in a nucleic acid molecule in the appropriate positions relative to the coding sequence so as to enable expression of the coding sequence. This same definition is sometimes applied to the arrangement of other transcription control elements (e.g., enhancers) in an expression cassette. Transcriptional and translational control sequences are DNA regulatory sequences, such as promoters, enhancers, polyadenylation signals, terminators, and the like, that provide for the expression of a coding sequence in a host cell.
The terms “promoter,” “promoter region,” or “promoter sequence” refer generally to transcriptional regulatory regions of a gene, which may be found at the 5′ or 3′ side of the coding region, or within the coding region, or within introns. Typically, a promoter is a DNA regulatory region capable of binding RNA polymerase in a cell and initiating transcription of a downstream (3′ direction) coding sequence. The typical 5′ promoter sequence is bounded at its 3′ terminus by the transcription initiation site and extends upstream (5′ direction) to include the minimum number of bases or elements necessary to initiate transcription at levels detectable above background. Within the promoter sequence is a transcription initiation site (conveniently defined by mapping with nuclease S1), as well as protein binding domains (consensus sequences) responsible for the binding of RNA polymerase.
The term “nucleic acid construct” or “DNA construct” is sometimes used to refer to a coding sequence or sequences operably linked to appropriate regulatory sequences and inserted into an expression cassette for transforming a cell. This term may be used interchangeably with the term “transforming DNA” or “transgene”. Such a nucleic acid construct may contain a coding sequence for a gene product of interest, along with a selectable marker gene and/or a reporter gene. The term “selectable marker gene” refers to a gene encoding a product that, when expressed, confers a selectable phenotype such as antibiotic resistance on a transformed cell. The term “reporter gene” refers to a gene that encodes a product which is easily detectable by standard methods, either directly or indirectly.
A “heterologous” region of a nucleic acid construct is an identifiable segment (or segments) of the nucleic acid molecule within a larger molecule that is not found in association with the larger molecule in nature. When the heterologous region encodes a plant gene, the gene will usually be flanked by DNA that does not flank the plant genomic DNA in the genome of the source organism. In another example, a heterologous region is a construct where the coding sequence itself is not found in nature (e.g., a cDNA where the genomic coding sequence contains introns, or synthetic sequences having codons different than the native gene). Allelic variations or naturally-occurring mutational events do not give rise to a heterologous region of DNA as defined herein. The term “DNA construct” is also used to refer to a heterologous region, particularly one constructed for use in transformation of a cell.
The term “vector” is intended to refer to a nucleic acid molecule capable of transporting another nucleic acid to which it has been linked. One type of vector is a “plasmid,” which refers to a circular double-stranded DNA loop into which additional DNA segments may be ligated. Another type of vector is a viral vector, where additional DNA segments may be ligated into the viral genome. Certain vectors are capable of autonomous replication in a host cell into which they are introduced (e.g., bacterial vectors having a bacterial origin of replication and episomal mammalian vectors). Other vectors can be integrated into the genome of a host cell upon introduction into the host cell, and thereby are replicated along with the host genome. Moreover, certain vectors are capable of directing the expression of genes to which they are operatively linked. Such vectors are referred to herein as “recombinant expression vectors” (or simply, “expression vectors”). In general, expression vectors of utility in recombinant DNA techniques are often in the form of plasmids. In the present specification, “plasmid” and “vector” may be used interchangeably as the plasmid is the most commonly used form of vector. However, the invention is intended to include such other forms of expression vectors, such as viral vectors (e.g., replication defective retroviruses, adenoviruses and adeno-associated viruses), which serve equivalent functions.
“Percentage of sequence identity” is determined by comparing two optimally aligned sequences over a comparison window, where the portion of the polynucleotide sequence in the comparison window may comprise additions or deletions (i.e., gaps) as compared to the reference sequence (which does not comprise additions or deletions) for optimal alignment of the two sequences. The percentage is calculated by determining the number of positions at which the identical nucleic acid base or amino acid residue occurs in both sequences to yield the number of matched positions, dividing the number of matched positions by the total number of positions in the window of comparison, and multiplying the result by 100 to yield the percentage of sequence identity.
The term “substantial identity” of polynucleotide sequences means that a polynucleotide comprises a sequence that has at least 25% sequence identity compared to a reference sequence as determined using the programs described herein; preferably BLAST using standard parameters, as described. Alternatively, percent identity can be any integer from 25% to 100%. More preferred embodiments include polynucleotide sequences that have at least: 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% sequence identity compared to a reference sequence. These values can be appropriately adjusted to determine corresponding identity of proteins encoded by two nucleotide sequences by taking into account codon degeneracy, amino acid similarity, reading frame positioning, and the like.
The term “substantial identity” of amino acid sequences (and of polypeptides having these amino acid sequences) normally means sequence identity of at least 40% compared to a reference sequence as determined using the programs described herein; preferably BLAST using standard parameters, as described. Preferred percent identity of amino acids can be any integer from 40% to 100%. More preferred embodiments include amino acid sequences that have at least 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% sequence identity compared to a reference sequence. Polypeptides that are “substantially identical” share amino acid sequences as noted above except that residue positions which are not identical may differ by conservative amino acid changes. Conservative amino acid substitutions refer to the interchangeability of residues having similar side chains. For example, a group of amino acids having aliphatic side chains is glycine, alanine, valine, leucine, and isoleucine; a group of amino acids having aliphatic-hydroxyl side chains is serine and threonine; a group of amino acids having amide-containing side chains is asparagine and glutamine; a group of amino acids having aromatic side chains is phenylalanine, tyrosine, and tryptophan; a group of amino acids having basic side chains is lysine, arginine, and histidine; and a group of amino acids having sulfur-containing side chains is cysteine and methionine. Preferred conservative amino acids substitution groups are: valine-leucine-isoleucine, phenylalanine-tyrosine, lysine-arginine, alanine-valine, aspartic acid-glutamic acid, and asparagine-glutamine.
INCORPORATION BY REFERENCE
All of the U.S. patents, U.S. published patent applications, and published PCT applications that designate the U.S. cited herein are hereby incorporated by reference.
EQUIVALENTS
While several embodiments of the present invention have been described and illustrated herein, those of ordinary skill in the art will readily envision a variety of other means and/or structures for performing the functions and/or obtaining the results and/or one or more of the advantages described herein, and each of such variations and/or modifications is deemed to be within the scope of the present invention. Those skilled in the art will recognize, or be able to ascertain using no more than routine experimentation, many equivalents to the specific embodiments of the invention described herein. It is, therefore, to be understood that the foregoing embodiments are presented by way of example only and that, within the scope of the appended claims and equivalents thereto; the invention may be practiced otherwise than as specifically described and claimed.








Organism Name: Corchorus olitorius



Type: DNA


Length: 3639


Sequence Name: TIR-NBS-LRR1 resistance protein; complete cDNA


SEQ ID NO: 1



ATGCTGTCCATACAATCATCTTCTTCTTGTTCCTATGTTTCTCGAAAGAAGTATGATGTTTTCCTGAGTTT



 


CAGAGGAGAAGATACCCGCAATAACTTCACCGATCATCTTTATGCCGCTCTAGTCAGGAGAGGAATCG


 


TCACTTTCAGGGACAATGAAAGGCTCGAGGCCGGAGAATCGATCGCACCGGAACTCTTTAAAGCAAT


 


TCAGGAATCATGGGGTTCAGTAATTGTATTCTCGGAAACTTATGCCTTTTCAGGTTGGTGCTTGGACGA


 


GCTAACCCAGATTGTCAAACAGAAAAATGAAGAAGGGCATAAAGTTTTCCCCATTTTCTATGATGTTGA


 


TCCATCTGATTTAAGAAAACAGACAGGGAAAGTTGCAGAAGCCTTTGTCAAACATGAAGAAAGATATA


 


AGGAGAATAAAAACAAGACACAAACTTGGCGATCTGCTTTGACTGAAGTGGCTAATTTAAAGGGATG


 


GCATCTAAATAATAGACCCGAAGCAGAATTCATTGCAGACATCGTTAAAAGGGTGTCAGCAAAGTTAT


 


ACCAAGCCTGTTCTAGCATTCCTGATGACTTAATTGGAATTCATTCACGCTTGGAAGAGTTGCATTCCA


 


AACTAGAAATTGGGGAAGATGATATCCGCATTATAGGAATTTGTGGTATGGGTGGCATTGGCAAAACA


 


ACTCTCGCAAGAGTTGTTTACACTCAAATGTCACCTCATTTTGAAGCCAAAAGTTTTCTTTCTGATGTC


 


CGAGAAGTTTCAGATAAATTTGGACTTGTAGCTATACAGAAACAGCTTCTTTCTCAAATCTTCCCAGAG


 


GAACACCTCAATTTTTTTGATGTCCAAGAAGGAAGTTTCATGATAAGTCGTAGCCTATCTCACAAAAAA


 


GTTCTTATAGTTCTTGATGATGTTGATAACATACAACACTTGAAAATGGTTGGGAGCAGGATCATCGTA


 


ACTACAAGAGATGAACATGTGCTACAATCTTTTCAAGTGGATGATGTGTTAAAGCCTACAATATTAGAT


 


GCCAATGAAGCACTTCGTCTTTTTAGCTTAAAAGCTTTCAATAGTGATACACCAGAAGATGATTTCATT


 


GAGCTTTCTAAATGTGTTGTAGAATATGCTGATGGCCTTCCCTTAGCTCTTGAAGTATTGGGTTCATTTT


 


TATGTGGTAGGGATGAAGATCAATGGACAAGTGCCATTGAAAGGTTTAAAAGAGACTCTAACAAAGA


 


AATTCACAACCGGCTTCGAATAAGCTTTGATGGATTAGAAGAAACTGAGAAAAATATTTTTTTGGATAT


 


TGCATGTTTCTTTAAAGGGGAGGAGAAAGATTTTGTACTGAGAGTACTGGATGGTTGTGGGTTTTTCC


 


CAGGTATTGGAATAGATGCTCTCATTAAGAAATCTCTCATAAAAGTTTATGGGGACAAGGACAAATATT


 


TGTGGATGCATGACTTGCTACAAGAGATGGGAAGGAAAATTGTGAAGCAAAAATCGCTAGAAGAACC


 


TGGAAAACGTTGTAGATTGTGGGAGGGAAGGGATGTCTATGACGTGCTAACAAAGAACACTGCTACA


 


GAAGAAATTGAGGGCATGGACATCGACATCAAGTGTTGGGATCAGAGAAAGACAATCACTTGGAATG


 


TTGAAGCCTTCTTGAAGATGAAAAAATTAAGATTGCTCAGAGTCTCTTATCTCCCAAATCCCTGTGATCT


 


CAATTATCTTTCTGATAAGCTACGACTTTTAGATTGGAGTGGATATCCTTTTAGATCCTTACCTTCAAACT


 


TCCAACCAGACAACCTTGTTGCACTTCTCCTACCTTATAGCCGCGTTCAACAGCTATGGAATGGAAACA


 


TATGTTTAGAAAAGTTGAAATGGGTTAACCTCGAAGGCTCTGGAAACCTGACCAAGACCCCAGACTTT


 


ACAATGGCTCCAAATCTCGAAACTTTGATTTTGGAAGCTTGTATCAAGATAGTAGATGTTCATCCCTCCA


 


TTGGACTTCTGAGGAGACTCAGATTTCTGAATTTAAGAAACTGCAAAAGTCTTAGGAGACTTCCAACC


 


AAAATTGGCATGAAATCTCTTGAAACATGGATTCTTTCAGGTTGCTCAAATCTTGAAAGGTTACCAGAT


 


CAGATTGATGGGGAAATGGAATGTTTAGTTGAGCTTTATTTAGATGGGACGGGCATTCGACATCTTCC


 


CTCTCTAATTGGACATCTGAGTGGTCTTGTTTTATTAAATCTGAAAGGTTGCAGGAACTTGGCGAGCCT


 


CCCAAGCAACATTAATGGGTTGAAACGCTTAAAAATTTTTGATCTCTCTGGCTGCTCTAAACTTGAAAT


 


TTTGCCAGAAAGTTTGCAGCAAGTAGAATCTTTGGAGGAGCTTGATTTAAGTGAAACTGCCATAAGAC


 


AACCGCCATCCTTCATATTTCAATTTAAAAATCTTAAACATCTGTCTTTCCGTGGATGCAAGGGGCCACT


 


GTCTAAATTAAGACCAAATCTGCCTTCTCTTTTCAAGGTTATGCAAAGCAGAAGTTTGAATTCCATGGC


 


TCTAATGTTACCTCCTTTGTCAGGTTTGAGTTCTTTAACAAATCTGGATATAAGTTACTGCAATCTTGGT


 


GAAGAGGCTATTCCTAGCGATGTTTACCGGTTATCCTCTTTGAAAAAATTAAATCTTTGTGGTAACAATT


 


TCATCAGCCTGCCTGCAAATCTTGAACGACTTTCCAATCTTAAGTGTCTCGTATTGACACATTGTATGGA


 


GCTTAAATCATTGCCTGAGTTTCTAACAAGCACGGCCAGTTCATGCAATATTATAGGTCGTCATAGTGTA


 


GACCTATCTGCAAATGCAACAGTACGCAACTCAGTAAGCTGTGCTTCTATTTGGTTAACTAATTGCTTCA


 


GATTGAGTGAGAATACAGACATAGTAACATTGCTGAAAAAACATCTTAAGGCATCTGCAAATTCAAGA


 


CAATTGAACATTGTTCTACCCGGAAGTGAAATCCCAGAGTGGTTCAGCAATCAGAGGGATGGATGTTC


 


GATAAAGATACCTCTGCCTTACCAGATTTTGAATGATAGTCAGTGTATTGGAGTTGCTTTCTGCTGTGTC


 


TTTGTCAATGCTATAGAAATGCGCCGCAAAGCTTTTATCCATGGTAGAAAGTCTCAAAATGTGGATAAT


 


CATGTGTTGTGCATTACAAATGGCTGCTCCTCGGTCACTAAAGACCACCTCTTGCTAGGTTATTGGTCT


 


CGTGACTACTTTTATTCAATTTATTCCTTGGAGGAGAAATGTGGTGAAACTGAGCAATTATCAAGCCTA


 


GAATCCGATGAACTTGAGGTTGTGGTTGAGGTTGATGAGGATGAGATGTTGTCATCTAAGCCGACCAT


 


CAAGAAGTGTGGAATTCATATAGTTTATAAGAAAGATGTGGAAGAGATGGAACAAATAAAAGAACACC


 


ACATTCTGCAAATTGGCAATACAACTATTGAGGATATCCCTCAGCCTCAGAACGGTGATGAATCGGAG


 


ATAGGGAAGGGAGCTCTTGTAAAGCGAAAACGCAACTTCTATGAGAAAAGTGAGAGTGACAAAATTG


 


AAGAGAGACCACAACCTAAACGGCTTCAACAATTTCTAAAATGTATAATGCGGAAGGAGCTTTAA


 


Organism Name: Corchorus olitorius


Type: PRT


Length: 1212


Sequence name: TIR-NBS-LRR1 resistance protein peptide


SEQ ID NO: 2



M L S I Q S S S S C S Y V S R K K Y D V F L S F R G E D T R N N F T D H L Y A A L V R R G I V T F R



 


D N E R L E A G E S I A P E L F K A I Q E S W G S V I V F S E T Y A F S G W C L D E L T Q I V K Q K


 


N E E G H K V F P I F Y D V D P S D L R K Q T G K V A E A F V K H E E R Y K E N K N K T Q T W R


 


S A L T E V A N L K G W H L N N R P E A E F I A D I V K R V S A K L Y Q A C S S I P D D L I G I H S


 


R L E E L H S K L E I G E D D I R I I G I C G M G G I G K T T L A R V V Y T Q M S P H F E A K S F L S


 


D V R E V S D K F G L V A I Q K Q L L S Q I F P E E H L N F F D V Q E G S F M I S R S L S H K K V L


 


I V L D D V D N I Q H L K M V G S R I I V T T R D E H V L Q S F Q V D D V L K P T I L D A N E A L R


 


L F S L K A F N S D T P E D D F I E L S K C V V E Y A D G L P L A L E V L G S F L C G R D E D Q W T


 


S A I E R F K R D S N K E I H N R L R I S F D G L E E T E K N I F L D I A C F F K G E E K D F V L R V L


 


D G C G F F P G I G I D A L I K K S L I K V Y G D K D K Y L W M H D L L Q E M G R K I V K Q K S L


 


E E P G K R C R L W E G R D V Y D V L T K N T A T E E I E G M D I D I K C W D Q R K T I T W N V


 


E A F L K M K K L R L L R V S Y L P N P C D L N Y L S D K L R L L D W S G Y P F R S L P S N F Q P D


 


N L V A L L L P Y S R V Q Q L W N G N I C L E K L K W V N L E G S G N L T K T P D F T M A P N L


 


E T L I L E A C I K I V D V H P S I G L L R R L R F L N L R N C K S L R R L P T K I G M K S L E T W I L


 


S G C S N L E R L P D Q I D G E M E C L V E L Y L D G T G I R H L P S L I G H L S G L V L L N L K G


 


C R N L A S L P S N I N G L K R L K I F D L S G C S K L E I L P E S L Q Q V E S L E E L D L S E T A I R


 


Q P P S F I F Q F K N L K H L S F R G C K G P L S K L R P N L P S L F K V M Q S R S L N S M A L M


 


L P P L S G L S S L T N L D I S Y C N L G E E A I P S D V Y R L S S L K K L N L C G N N F I S L P A N L


 


E R L S N L K C L V L T H C M E L K S L P E F L T S T A S S C N I I G R H S V D L S A N A T V R N S


 


V S C A S I W L T N C F R L S E N T D I V T L L K K H L K A S A N S R Q L N I V L P G S E I P E W F


 


S N Q R D G C S I K I P L P Y Q I L N D S Q C I G V A F C C V F V N A I E M R R K A F I H G R K S Q


 


N V D N H V L C I T N G C S S V T K D H L L L G Y W S R D Y F Y S I Y S L E E K C G E T E Q L S S L


 


E S D E L E V V V E V D E D E M L S S K P T I K K C G I H I V Y K K D V E E M E Q I K E H H I L Q I


 


G N T T I E D I P Q P Q N G D E S E I G K G A L V K R K R N F Y E K S E S D K I E E R P Q P K R L Q


 


Q F L K C I M R K E L


 


Organism Name: Corchorus olitorius


Type: DNA


Length: 3621


Sequence Name: TIR-NBS-LRR2 resistance protein; complete cDNA


SEQ ID NO: 3



ATGTTGTCCTTACCACCATCATCCTCTTCCCATGTTTCTCGAAAAAAGTATGATGTTTTCTTGAGTTTCAG



 


AGGAGCAGATACTCGCCAAAAGTTCACCGATCATCTCTATGCTGCCTTGAAAAGAAATGGAATCATCA


 


CTTTCAGGGACAATGAAAGGCTTGAGGCTGGTGAATCGATCAGGATGGAACTCTTTAAAGCAATTCA


 


GGAATCATGGTGTTCTATAGTTGTATTCTCAAAAACTTATTCCTTTTCAGGTTGGTGCTTGGACGAGCTT


 


GCTGAGATTGTCAAACAGAAAAATGAATGCAGGCATACAATTTTCCCCATTTTCTATGATATCGATCCAT


 


CTGATCTAAGAAAGCAGACGGGAAGAGTTGCAGAAGCCTTTGCCAAACATGAAGAGAGATACAAGG


 


AGAATAGAAACAGGACACAAAGCTGGCGATCTGCTTTAACTGAAGTGGCTAATTTAAAGGGATGGCA


 


TCTAAATAATACAAGACATGAATCAGAATTCATTGGAGACATTGTTAGAAGGATATCAGCAAAGTTATG


 


CCAAACCTATTCCTCTGTTCCAGATGACTTGATTGGAATTAATTCAAGTTTGGAAGAGTTGCATTCTAA


 


AATAGACATTGGGGAAGATGACATTCGTATTATAGGAATTTGTGGTATGGGTGGCATTGGCAAAACAA


 


CTCTTGCAAGAGTTGTTTACACTCAAATGTCACCTCATTTTGAAGGTAAAAGCTTTCTTCCCGATGTTC


 


GAGAAGTTTCAAATAAACTTGGACTTGTATTTTTACAGAAGCAGTTTCTTTCTCACATTTTCCCAGAAG


 


AATGCTTCAATTTTTCTGATGTTCATGAAGGAAGTTACATGATTAATCGTAGGCTATCTCACAAAAAGGT


 


TCTTGTAGTTATCGATGATGTCGATAACATACAACAGTTGAAATGGTTGATTGGAAGGCGTGATTGGCT


 


TGGTTCAGGTAGCAGAGCCATTTTAACTACTAGAGATGAACATGTTCTGCTATCATACAGAGTGGATCA


 


TGTTTGTAAGCCAACAACATTAGATTCCAATGATGCACTTTGCCTTTTTAGTTTGAAAGCTTTCAATAAT


 


GATACACCAGAAAATGATTTCATTGAGCTTTCTAAACGTGTTGTACAATATTGTGATGGCCTTCCCTTAG


 


CTCTTGAAGTTTTGGGTTCATTTTTTTGTGGAAGAGATGCAGCTCAATGGAGAAGTGCAATTGAAAG


 


GCTTAAAAGAGAGTCTAACAAGGAAATTCATGACCGGCTTCAAATAAGCTTTGATGGATTAGAAGAAA


 


CAGAGAAAAACATATTTTTGGATGTTGCTTGTTTCTTTAAAGGGGAGGAGAAAGATTTAGTAATCAAA


 


GTTCTAGATGGTTGCGAGTTTTACCCAGATATTGGAATAGATGTTCTCATCAAGAAATCTCTCATCAAAT


 


TTTATGGTGACAAGTATTTGGGGATGCATGATTTGCTGCAAGAGATGGGTAGAAAAATTGTCAAGCAA


 


AAATCTGTTGATGAACCTGGACGACGTTGTAGATTGTGGGAGGAAAGGGATGTCTATCATGTGCTAAC


 


AAAGAACACGGCTACGAAAGCAGTTGAAGGCTTGGACATCAACGTTAAATGCTGGGAGCACAGAAA


 


GATGTTCACTAGGAATGCTGATGCCTTCATGAAGATGAAAAAATTAAGATTGCTCAAAGTCTGTAATCT


 


CCCAAATTCTCATGATCTCAAATATCTTTCTAATGCGCTACGGCTTTTAGATTGGACTGGATATCCTTTCA


 


GATCCTTGCCTTCACGCTTCCAACCAGACAACCTTGTTGCACTTCTCCTACCTTGTAGCCGCATTGAAC


 


AACTATGGAACGGAAACATACTTTTAGAAAAACTGAAATTCGTCAACCTCGAAGGATCCATGAACCTG


 


ATCAGGACACCAGACTTTACAATGGCCCCAAATCTGGAAAGTTTGATTTTGGAAAGTTGTGTCAACTT


 


AGTAGATGTTCATCCATCCATCGGCCTTCTAAGGAGACTAAAACTTCTGAATTTTAGAGGCTGCAAAAG


 


TCTTAGCAGTCTTCCAACCAAAATTGGAATGAAATCTCTTGAAACATTGATTCTTTCAGGTTGCTCAAAT


 


CTTGAAAGGTTACCAGATCAGATTGATGGGAAGATGGAATGTTTGGTCGAGCTTCATTTAGATGGGAC


 


CGGTGTGGGACATCTTTCCTCTGCAATTGGACATCTGAGTGGTCTTGTTTTATTGAATCTGAAAGATTG


 


CAGAAATTTAGCAAGTCTCCCAAGCAGCATTAATGGGCTGAAATGTCTGAAAACTCTTAATCTCTCAGG


 


CTGCTCTAATCTTGAACATTTTCCAGAGAATTTGCAGCAATTAGAATCTTTAGAGGAGCTTGATTTAAG


 


TGGAACTGCTATAACAAAGCCACCATCCTTCATTTTCCAATTCAAGAATCTTAAACATCTGTCTTTCCAT


 


GGATGCAAGGCACCACCAACTAAATTACAACCAAATCAGCCTTCTCTAGGATGTATGAACTGCATGGC


 


GCTAACCTTACCGCCTTTGTCAGGTCTGAGTTCTTTAACACAGCTGAATATAAGTTACTGCAATCTTTAT


 


GAAGGAGCTATTCCGAGTGATATTTGCTCCTTATCCTCTTTGAAAAGACTAGATCTTCGCGGTAACAAT


 


TTCTTCAGCCTACCTGCGAATCTTGATAGACTTTCCAATCTCGACTATCTCGGATTGACAGATTGTATGG


 


AGCTTAAATCATTGCCTGAGCTTCTAACAAGCACACTAGTTCCTATTTCAAATGATTGCAGTTTTCCAGT


 


GGGACTATTTGCAAATGCAAGAGCATGCAATTCAATGGATTGGGCACCTGCTTCTATTTGGTTAACTAA


 


TTGCTACAGACTGGCTGAGAACACAAATGTATTAACATTGCTGAAAAAACATCTTAAGGTGTTTGCAA


 


AGGCAAGAGAAACTTTGGACATTATTTTACCCGGAAGTCAAATCCCAGATTGGTTCAGCCATCAGAGC


 


AATGAATCGTCAATCAAGATACCTCTGCCTCACCATCTTCAGAGTAATAGTAAGTGGATTGGAGTTGCT


 


TTCTGCTGTGTCTTCGTCGATGTTGTTGGTATCGACTGCAAAGCTTTTGTCCATGGTAGAATGTCCCAC


 


GACATTAATGGTTACGGGTTGTATTTTGGACATGGCTCCTCGGTCACAAAGGATCATCTTTGGCTACGT


 


TATTGGTCTCGTAACAAGTTATATTCGTTTGCCTTGGATGACAAATGTGGTGAAACAGGGCATCCACAG


 


AGCCTAAAATGTCCGGTAGATCAAGAATCCGATGAATTCGAGGTTGCTGTTGAGGTTGAGGTTGAGTT


 


GTCACGGTCACGTTTTAAGAAAGTGAAGAAGTGTGGGGTTAGACTAGTTTATGAGAAAGATTTGCAA


 


GAGTTGGAGCAACTTCTGCAAATCTGCAATTCAACTTGTGCAGATGAATCAAAAACAGGGGAGGTTC


 


CGGTAAAGCGAAAACGCAACATCTATGAAGAGGAGGCAGAACTAAGTGAGAGTGACAGCTTCCGAG


 


GGCCGGAACGATTTCTAAGATATATAATGCAGAAGAAGGAACATAATTGA


 


Organism Name: Corchorus olitorius


Type: PRT


Length: 1206


Sequence name: TIR-NBS-LRR2 resistance protein peptide


SEQ ID NO: 4



M L S L P P S S S S H V S R K K Y D V F L S F R G A D T R Q K F T D H L Y A A L K R N G I I T F R D



 


N E R L E A G E S I R M E L F K A I Q E S W C S I V V F S K T Y S F S G W C L D E L A E I V K Q K N


 


E C R H T I F P I F Y D I D P S D L R K Q T G R V A E A F A K H E E R Y K E N R N R T Q S W R S A L


 


T E V A N L K G W H L N N T R H E S E F I G D I V R R I S A K L C Q T Y S S V P D D L I G I N S S L


 


E E L H S K I D I G E D D I R I I G I C G M G G I G K T T L A R V V Y T Q M S P H F E G K S F L P D


 


V R E V S N K L G L V F L Q K Q F L S H I F P E E C F N F S D V H E G S Y M I N R R L S H K K V L V


 


V I D D V D N I Q Q L K W L I G R R D W L G S G S R A I L T T R D E H V L L S Y R V D H V C K P T


 


T L D S N D A L C L F S L K A F N N D T P E N D F I E L S K R V V Q Y C D G L P L A L E V L G S F F


 


C G R D A A Q W R S A I E R L K R E S N K E I H D R L Q I S F D G L E E T E K N I F L D V A C F F K


 


G E E K D L V I K V L D G C E F Y P D I G I D V L I K K S L I K F Y G D K Y L G M H D L L Q E M G R


 


K I V K Q K S V D E P G R R C R L W E E R D V Y H V L T K N T A T K A V E G L D I N V K C W E H


 


R K M F T R N A D A F M K M K K L R L L K V C N L P N S H D L K Y L S N A L R L L D W T G Y P F


 


R S L P S R F Q P D N L V A L L L P C S R I E Q L W N G N I L L E K L K F V N L E G S M N L I R T P


 


D F T M A P N L E S L I L E S C V N L V D V H P S I G L L R R L K L L N F R G C K S L S S L P T K I G


 


M K S L E T L I L S G C S N L E R L P D Q I D G K M E C L V E L H L D G T G V G H L S S A I G H L S


 


G L V L L N L K D C R N L A S L P S S I N G L K C L K T L N L S G C S N L E H F P E N L Q Q L E S L E


 


E L D L S G T A I T K P P S F I F Q F K N L K H L S F H G C K A P P T K L Q P N Q P S L G C M N C


 


M A L T L P P L S G L S S L T Q L N I S Y C N L Y E G A I P S D I C S L S S L K R L D L R G N N F F S


 


L P A N L D R L S N L D Y L G L T D C M E L K S L P E L L T S T L V P I S N D C S F P V G L F A N A


 


R A C N S M D W A P A S I W L T N C Y R L A E N T N V L T L L K K H L K V F A K A R E T L D I I L


 


P G S Q I P D W F S H Q S N E S S I K I P L P H H L Q S N S K W I G V A F C C V F V D V V G I D C


 


K A F V H G R M S H D I N G Y G L Y F G H G S S V T K D H L W L R Y W S R N K L Y S F A L D D K


 


C G E T G H P Q S L K C P V D Q E S D E F E V A V E V E V E L S R S R F K K V K K C G V R L V Y E


 


K D L Q E L E Q L L Q I C N S T C A D E S K T G E V P V K R K R N I Y E E E A E L S E S D S F R G P


 


E R F L R Y I M Q K K E H N


 


Organism Name: Corchorus olitorius


Type: DNA


Length: 719


Sequence Name: TIR-NBS-LRR3 resistance protein; partial cDNA


SEQ ID NO: 5



TTGCGAGTCTCCCAAGCAGCATTCATGAGTTGAAATGTCTAAAAACTCTTGATCTCTCAGGCTGCTCTA



 


AACTTGAAAATTTGCCAGAGAGTTTGCAGCAAGTAGAATCTCTCGAGGAGCTTGATTTAAGTGGGAC


 


TGCCATAACAAAACCACCATCCTTCATTTTTCAATTGAAAAATCTTAAACATCTGTCTTTCCGCGGATGC


 


AAGGGAACAGTTTCTAAATCACGACCCAATCTGCTTTCTCTTTTCAAGGTAATGCAAAGAGGAGGAG


 


GAAGTGTGAATTCAGTGGCTCTAACCTTACCTCCCTTGTCAGGTTTGACTTGTTTGACAAAGCTGGATA


 


TAAGTTACTGCAATCTTGGTGAAGGGGCTATTCCTAGCGATATTTGCCACTTATCCTCTTTGAGAGACTT


 


GAATCTTAGTGGTAACACTTTCTCCAGCCTTCCTGCAAACCTTGATCGACTTTCCAATCTTGAGCGTATC


 


AGACTGAGACATTGTACGGAGCTTAAATCATTGCCTGAGCTTCTTAGGAGCACATACCATTCGGTTGG


 


AGTATTTGCGAATGCTGCAATACGCAACTCGCGAGATTGGGCATGTATTTTTTTACCTAATTGCTACAG


 


AATAGCTGAGAATACAAATATAGTAACCTTGCTGAAGAAGAATCTTAAGGTTGGTCTCTATATCTCTCCC


 


CCTAATCAGCTCACACAAAGAAATTTATGA


 


Organism Name: Corchorus olitorius


Type: PRT


Length: 238


Sequence name: TIR-NBS-LRR3 resistance protein peptide


SEQ ID NO: 6



A S L P S S I H E L K C L K T L D L S G C S K L E N L P E S L Q Q V E S L E E L D L S G T A I T K P P S



 


F I F Q L K N L K H L S F R G C K G T V S K S R P N L L S L F K V M Q R G G G S V N S V A L T L P P


 


L S G L T C L T K L D I S Y C N L G E G A I P S D I C H L S S L R D L N L S G N T F S S L P A N L D R


 


L S N L E R I R L R H C T E L K S L P E L L R S T Y H S V G V F A N A A I R N S R D W A C I F L P N


 


C Y R I A E N T N I V T L L K K N L K V G L Y I S P P N Q L T Q R N L


 


Organism Name: Corchorus olitorius


Type: DNA


Length: 2583


Sequence Name: NBS-LRR resistance protein; complete cDNA


SEQ ID NO: 7



ATGGCTGAAGCTGCTGTTTCCTTTGTGCTAGAGAGGCTAGCTGACATACTGGAAGAAATTGATTTTCA



 


AACAAATGTTCGGAATGAAGTTGTGCGTTTACAAGATGAACTGAAGAGGATGCGTTGTTTCTTGCGTG


 


ATGCAGATGCAAAGCAAGATGATGATGATAGAGTGCGCAACTGGGTGTCTGATATTAGAAATGTAGCT


 


TATGATGCTGAGGATCTCATTGACACATTCATTCTCAGAATTGATGCCGTTCAAAAAAAGAACTCCATC


 


AAAAAGTATGCTTCCGTTTTCAAAGATTGGAAACGTCGCTCCAAGATTGCAAATGAGCTCATTGCTATC


 


CAGAGAAGAATCCTTGATGTTTCTCAGAGTCGTGAAAAGTATGGTATCAAGAACATTGGAGAGGGGA


 


TTAGCACAGCAAAAGAGAGGCTCCGCAAGCAGAGAAGATCATCTCCTCGCGGTGAAGAGAAAGATA


 


TTGTTGGACTGGACGATGATATAGCTAAGCTGGTGACACAACTTGTTGATGCTGAGGACCAATGGCAT


 


GCCATTTCAGTAGTGGGAATGGGAGGGATCGGCAAGACAACTCTTGCCAAGAAGGTTTACAATCATG


 


CTGATATCCAGGCCCGTTTTCCTACCCGAGCATGGGTTTATGTATCCCAGGAATACAGCATTCGAGACA


 


TATTTCAGGCAATTATAAAGCAAGTGGCATCAACAGGAAGGAATTTGGAAAAACTGCGGGAAGAAGA


 


GTTGGAAGAAATTCTCTATGAACACCTCCGGAAAAAACGGTATTTGGTGGTCTTGGATGATGTATGGA


 


GCATAGAAGCATGGAATTCTCTTTCTGAGGCCTTTCCGGATAGCAGCAGCAATGGAAGCAGAGTGAT


 


GCTAACGACTCGCAACAAGAGTATTGCTCTCAAAGCAGATGCTAGAAGTGTTCCTTATGATTTGCACTT


 


TATGAATGAAGAAAATGGATGGATGTTGTTCTGCAAGAAAGCTTTCATTCAAAGTGCTGATTCACATCG


 


TTCCCCACGTTTGGAGGAAATCGGGAAGGAGATTGTTGAAAAATGTGCTGGTTTACCACTGGCCATCA


 


TTGTGATGGGAGGATTGCTTTCAACAAAAAGAAGTTTAGCAGAATGGAAAAGGGTTCTCTCCAACAT


 


GAGCTCATTCTTTGCTCAAGACCCGAATGGGGTATCAGCAATACTGGCTTTGAGTTACAACGACTTGC


 


CATATTATCTCAAATCTTGTTTCCTCCATCTAGGACAGTTCCCAGAAGACCAGCCAATTCCAACACATAA


 


ATTGTTTAGGCTATGGATTGCTGAGGGCTTGATACCACAGCAAGGTGAAAGGGTGGAGGATGTAGCA


 


GAGGACTACTTGAATGAGCTAATAGAGAGAAACATGGTTCAAGTAGCCAAATGGAGTGTCAACGAGA


 


GAGTTAAACAATGTCGTCTTCATGATCTATTACGAGATCTCTCCATTTCAAAGGCCAAAGCAGAGAGCT


 


TTCATGAGATTCAAGGGAGCCAAAGCCTCGAACCTTCTGCTAGATCACGTCGTCATGCCATCTATTCCA


 


CCTTTCATTGGCCCCAATGTAAGTATTCCAATCCTCAACTTCGGACACTTCTCCTATTTAGAGTTGATCAT


 


AACCAAAGCCAGGTTAATTATTATATAAATGATCCCTATAAAATGGAAGGCAGCGATCTAGATTATATTA


 


GCAAAAACTTCAAATTACTGAGGGTCTTGGAGTTGGAGGGTATACCATGTGCTACCATTCCAAGCATA


 


ATTGGGTTACTAATTCATTTGAAGTACTTGGGGCTAAAGGAGACTAACCTGCAAGAGCTTTCATCCGC


 


CATTGGTTCTTTGAGGAGCCTGCAGACTCTTGATATAGCTGCAAATCTTCATCTTCTAACAATTCCCAAT


 


GTCATATGGAAGTTAAAAAGATTAAGGCATCTTTACATGTGCGGGCATAAATATGGGGGGCCTCTGCG


 


AATCGACACATTACAGCATCTTCAAGCTCTGTCTGAAATAAATGTCCAGAGATGGATGCAAAATGATCC


 


TGCCAATTTAACCAGCCTGCGAACTGAAGAGGCTGATTTTCCATCTCTCACACAACTTTCTGCTCTTCA


 


AAATCTTGTCAAGTTGCATATGAGAGGAACAATAAGGCAGTTGCCAAACTCAGAGGAATTCCCACCAA


 


ATCTCTGTCAGCTGACCTTGGAACATACCCATCTCCAGCAAGATTCAGTGGGAATTCTTGAGAAATTGC


 


CAAGATTGTTGATTTTGAGACTAAAAGCACGGTCCTACGATGGAGAAAAAATGAAAATATCAGTCAGC


 


GGCTTTCCCCAACTTGAAGTCCTGGAGCTTGTTTCATTGGAATCATTGGAAGAGTTGAATCTTGAAGA


 


AGGTGCAATGCTAAGGCTTAGGAGTTTTCGGATTATAAAGTGTGAGAAATTGAAGATGCTTCCTGAGG


 


GAATGAAAACCCTAACCGGTCTCCGTGAGTTGGACATTGAATTGATGCCAAAATCATTCGTGGATAGG


 


ATTCGTGGGGAAGATTTCTACAAAGTGCAGCATGTTCCCTCTATCTTGTTTGTTTGA


 


Organism Name: Corchorus olitorius


Type: PRT


Length: 860


Sequence name: NBS-LRR resistance protein peptide


SEQ ID NO: 8



M A E A A V S F V L E R L A D I L E E I D F Q T N V R N E V V R L Q D E L K R M R C F L R D A D A



 


K Q D D D D R V R N W V S D I R N V A Y D A E D L I D T F I L R I D A V Q K K N S I K K Y A S V F


 


K D W K R R S K I A N E L I A I Q R R I L D V S Q S R E K Y G I K N I G E G I S T A K E R L R K Q R R


 


S S P R G E E K D I V G L D D D I A K L V T Q L V D A E D Q W H A I S V V G M G G I G K T T L A K


 


K V Y N H A D I Q A R F P T R A W V Y V S Q E Y S I R D I F Q A I I K Q V A S T G R N L E K L R E E


 


E L E E I L Y E H L R K K R Y L V V L D D V W S I E A W N S L S E A F P D S S S N G S R V M L T T R


 


N K S I A L K A D A R S V P Y D L H F M N E E N G W M L F C K K A F I Q S A D S H R S P R L E E I


 


G K E I V E K C A G L P L A I I V M G G L L S T K R S L A E W K R V L S N M S S F F A Q D P N G V


 


S A I L A L S Y N D L P Y Y L K S C F L H L G Q F P E D Q P I P T H K L F R L W I A E G L I P Q Q G E


 


R V E D V A E D Y L N E L I E R N M V Q V A K W S V N E R V K Q C R L H D L L R D L S I S K A K A


 


E S F H E I Q G S Q S L E P S A R S R R H A I Y S T F H W P Q C K Y S N P Q L R T L L L F R V D H N


 


Q S Q V N Y Y I N D P Y K M E G S D L D Y I S K N F K L L R V L E L E G I P C A T I P S I I G L L I H L


 


K Y L G L K E T N L Q E L S S A I G S L R S L Q T L D I A A N L H L L T I P N V I W K L K R L R H L Y


 


M C G H K Y G G P L R I D T L Q H L Q A L S E I N V Q R W M Q N D P A N L T S L R T E E A D F P


 


S L T Q L S A L Q N L V K L H M R G T I R Q L P N S E E F P P N L C Q L T L E H T H L Q Q D S V G


 


I L E K L P R L L I L R L K A R S Y D G E K M K I S V S G F P Q L E V L E L V S L E S L E E L N L E E G


 


A M L R L R S F R I I K C E K L K M L P E G M K T L T G L R E L D I E L M P K S F V D R I R G E D F


 


Y K V Q H V P S I L F V


 


Organism Name: Corchorus olitorius


Type: DNA


Length: 6459


Sequence Name: CC-NBS-LRR1 resistance protein; complete cDNA


SEQ ID NO: 9



ATGGAGTTTGTTGTAGGCATTGTTAGCTCTATTTTCACGCCTGCTGTACAATTGATTATCTCCCCCATCA



 


AAAACAAAATCAAATACATTTCCAATCATGAGAACAATGTCCAAACTCTCAAGAATCAAGTTGAGAGC


 


CTGAAGGATGAAAGAAAGAGAGTTCAACATTCTGTTGATGCCGCTAGACAAAACGGGGAAGAGATT


 


GAAGATGATGTTAAAAAGTGGCAGAAGACGGTCGACCAGAAGATCGCTGATGAAGTAGAGAAAGTA


 


ATTGCAGATGAGGAGAAAGCTAAGAAAAAATGTTTCGTTGGCTTGTGTCCGAATCTCTGGGCTCGTTA


 


TAAGCATAGTGTGAAAGCTGAAGAGAAGGGGAAGGTTGTGGCTAAGTTGCTTGAACAGGGCAAATT


 


TGACAAGGTTTCTTATCGTCCAGCTCCACAAGGTGCCAGTGTCACTGCTGCATTTGTTAAAGGTTTTG


 


AAGAATTCAAGTCAAGAGAAGTGCTTTTGAAAGGAATCATGGAGGCGCTGAACGATGATAAAATCAA


 


CATCATGGGAGTGCATGGCATGGGTGGCGTTGGCAAGACCATGCTTGTTAAAGAAGTTGCTAGACAA


 


GTTAAGGAGGGGAGGCTATTTGATTATGTGGTTATGGCAAAAGTGACTCAGACTGTTGATGTCAAGAC


 


AATTCAGAATGATATTGCTGAGTTACTGGGTTTAAGATTTGACGAACAGAGTATTGTTAGGAGGGCTG


 


ATCGACTGCGAGAAAGATTGAAGAGAGAGACAAAAGTTCTTGTTGTTCTGGATGACGTATGGGAAAG


 


ATTAGATCTGGAGGAAGTTGGGATTCCAGTTGCAGATGAACACAAGGGGTGCAAGATACTGCTGACG


 


TCCAGAGATCTCAATGTTTTATCCAATGGGATGAACAGTGAGAAAAATTTTGTGGTTGGCCTTCTCACT


 


GAAGAAGAAACCTGGAACCTTTTTAAGAAGAAGGCCGGTTATGTTGTTGAAAGTTCTGATATAAAGCC


 


TACGGCTATCGAGGTGGCAAAGAAATGTGCAAAATTGCCAATCGCCATCGCCACAGTTGCAGGGGCT


 


TTGAGGAACAAAGAGGCGTTCCATTGGAAGGATGCTCTGTGCCAATTACAGAAGCCTTCAACAGTAA


 


ACCTCAAAGGGGTAGCAACGACTGTACATTCAGCTATAAAGCTTAGCTACGATTTTTTAGAAAGCGAG


 


GAAGTTAAATTCACTTTCTTGCTTTGCTGTTTATTGGGCCGCAATGGTTTGATTGAGGACTTGTTGAAG


 


TATGTTATTGGTATGAGATTATTTCAAGGCATCACAATAGAGGAAACAAGAAACAGAGTATTGACTGTG


 


GTGAGTAATCTGAAAGCCTCTTGCCTGTTACTTGATAGCTATAATAATGAAAAGTTTGATATCCATGATG


 


TTGTTTGGGATGTTGCTCTATTGATTGCATCAAGAGACCGCCATATGTTTGTCTTAAGAGATGGTGAAG


 


AGCTAAAAGACTGGCCTACCCAGGAGATGAAGGAGAATTGCAGTGCGATTAACTTTCGTTGTCCTCGT


 


ATTATGACCGAGCTACCTGATGAGATGGAAGGTTTACGTCTTTCCTTATTGCGCTTGGACAATGTAGGC


 


GCATTGGAAATTCCAGCCAACTTCTTTAGACGGATGGAAAGACTCGATGTCTTACATTTCACTAGAATG


 


CATTTTTCCTCCCTACCTGTTTCAATTAGTCTCCTTACAAACCTTCACACACTGTGTCTCAGTGATTGTGC


 


ACTGCAAGATATAACCATTGTTGGAAAGCTGAAGAATTTAGAAATCCTTAGCCTTGCACGTTCAGTTAT


 


TGAAGCCCTGCCCGAGGAGACAGCGCAATTGACTCGGTTAAGGCTGTTAGATTTGAGTCATTGTTCTA


 


AACTTCAGCTCATTCCACCAAATGTTCTATCAAGTTTGTCCAAGTTGGAAGAATTATATTTGTACAATAG


 


CTTTGTTCAATGGGAAGGTGAAGTACATAGCAGCGGAAGGAGAAATGCTAGCCTTGATGAACTAAAG


 


CATTTGTCTCATCTTACCACTTTGTATGTTCATATCCCCAATGCCGAAATAGTTCCAAAAGATCTTTTCAT


 


TGAGAGATTGGAAAGATTCAGTATTTTGATTGAAGATGAGCGGCGTTGGTACAGTGAGTTTGAATACT


 


CAAGAACTTTGAAATTGAAGATATATACAAGCATTTATTTGGATCATGCGGTGAGAATGTTATTGAAGA


 


AAACTGAAGATCTACATCTATACCAACTTAAAGGTATCAAGAATGTGCTTGATGAGTTAATTGATGGTGT


 


AGAATTGCCGCATCTAAAGAACCTTCATATTCGCAATGGTTCGGAGGTCCAATATATCATGAGGAAGAA


 


AATTGAGTGCGCTCAATTAAAGTCCATGACACTTGAAGGTCTGCCGAAACTGATTAGCTTTTGGTTTG


 


AAGACAAAAGGTGTTCCACATCTCATGAGGAGCGAGCTACGAGTTCCAATCCCCTGCCACTTTTCAAT


 


AAACAGCTTGTGTTCCCTTGCTTGGAAAGCCTGCGATTGTCCTCAATTAATGCTGAAAGAATATGGCAC


 


AGCCCTCTTTCGGAGAATTGTACTTTTGCTGCAAATTTGAAAAGCTTGACGGTTGAAGGCTGTGGCGA


 


ATTGGAGCATCTATTATCACCCTCTGTTGCCAGAAGTCTTGTCCAGCTTACACACTTCGAGGTAGCAAG


 


ATGCCAGCGCCTAAGAGAGATAATATCTACAGAGGAAATAGAAGATGAGAGTGTTGCGATTTGTTTCC


 


CCCAATTAAACTCCTTAGAGATAAGAAGTCTCCAGAATCTAGCAAACTTTTGTGCAGGAAACTATAATA


 


TTGAATTCCCAGCACTTAAAGTATTGGAGGTTAATGGCTGCCCTGTATTAAAGGAATTCATTAGGGTGA


 


ATAAAAGTGAGTTTCATGTGCCGGCTCTCTTCAATGAAAAGGTTGCTCTTCCTAGCTTGGAAAGGATG


 


GAATTCTCCTACCTGAAAAATGTGAAGATGATATTTGACAAGCAACTTCTGGCAGGTTCCTTTTGCAAA


 


TTAAAAGCAATGTCAGTTTATCATTGTGATGCATTATTGACTATTTTTTCATCTAATATATTTGGAGCATTT


 


CAGAGTCTAGAAAATCTTGATGTGTACAGATGTAATTCACTTGAAATGATATTTGAGGTTGGAGGGTTA


 


AATATCAGAGAACCACACGTTGTACACTCTCAACTAAGATCTCTGTATATTTCCTCGCTGCCTGCATTGA


 


AGCATGTTTGGAATAAAGATCCCCAAGGAATTCTTTCCTTCCAAAATCTTCATACAGTAGATTTGTCTTT


 


TTGTCGGAATTTGAAAAGTCTATTTCCAGTGTCAGTAGCCAAACACCTTCAACAGCTCGAAAATCTGA


 


GACTGTGTAATTCTGCGGTGGAGGAGATTGTGTTTTCAGAAGAAGGATTAGAAGAGCCCATTGGGTT


 


CGAGTTTGCTCAACTGTCTTCCCTTGTGCTTTATAATCTAAGAGAGCTCAAATGCTTCTATCGAGGGCA


 


GCATACAATAGTGTGGGCGATGTTGAAAAAGTTGGAGACGGATCATTCTACTTTACTGAAGATAGTAG


 


GTAGTAATTCACAACATCTTGGCATCCAAGAAATGAATAGCAATGACCCACCAGAATGCACAACTGGA


 


CAACCACTTTTTTCGACTGAAAAGGTCATTCCCATTTTAGAGGAACTGCATTTGCGGTTAACAAACCCT


 


GATGACATTTCAAAGATATGTGATGGCCATTTTCTCCAAAGATTCTGCAATTTGGAAAGTTTAGAACTT


 


TCATCTTCTGAAGGAGATGATGCTCAGATACTACCAGATGCGGTGACACTTCCACGAATTAAAACATTA


 


ATATTGTCTTCTTGCAACTTTCTTAAGCATATATGGGAGAAGAAGGATTCAGAGCTAGGGCACATTCTT


 


CAAAAGCTCGAAATTCTTGAAGTTAACGAGTGTGGCGATTTGACAAGTTTTGGACCGTCCTCGGCATC


 


TTTTCAAAATCTCACAACTTTGGAAGTGACATACTGCAACATGATGATAAACTTGGCTACACCCTCAGT


 


AGTCCAGAATCTGGTACAATTAACAACCATGAGGATAGCATACTGCAGGGGAATGGCAGAAATAGTTG


 


CAAATGAGGGAGGTGAAGCAACACCAACATATGAGATCAATTTCAGCAAGTTGCAAAGTTTAGAACT


 


CAATCGGTTACATCGCCTCACAAGCTTTTCTCCAGGGAATTACACCATCAACTTTCCTTCCTTGCAAGA


 


ATTAATGAGAGTAAAAGAATCACATAATGATCGAAAAGGGCGTTGGGCCGGTGACCTTAATACCACCA


 


TACAACTTTTGTACAGCGTAAATGTTGTTGAGGGATACCATGGCATATGTAATTTGAAATTATCAGACAC


 


CTCTCCTGAGTTGATGGAAATATGGAATGGAAGGAACCCTCATGAAATTGTGGACTTGAAATTCCTTG


 


GACGTGTGGAATTTTGTAACTGTAGCAGCTTGAAATACATTTTTACTCTGTCCAGGTTGTTGAGCCTCA


 


AGCACCTATCTTATTTAGTAGTAAAAGAATGCAGTACTCTGAAAGAAGTTGTGATGGAACAGGAAATT


 


GAGGAGGAAGCAACAACCGATAACTTCATATTCCCTAATCTCCGGTACATTAAAATTGAGTCATGTTCC


 


AGCTTGAGATGCTTTTATTTGGGAAGTGGAGCTCTTGAAATTCCACGGTTGGAAATTATTGAGATAACT


 


GACTGTCCAAAAATGACTACGTTTGCTTCTTCATTCCCAAGAGATGAGGAGAAAGAGATTAGTGCTGA


 


TGGAAGTGAAAAAAGGGTTGGCCATGGCGACCTCAATATTGAACCGTTTTTCAGCGATAAGGTGGCC


 


TTGCCTAGTTTAGAAAGGTTGAGAATCAAAGGCATGGGGAAATGTAGAAAGATATGGCAAGACCAAC


 


TCACTGTGAATTCATTTTGCGAGCTAAAGTATATACTGGTAGAAAGCTGTGAAAAACTCTCAAATATTTT


 


TCCATTCAACATGATGGAAAGGCTTGAGAAACTAGAAGAGTTGCAGATTGTGAATTGTGATTCATTAG


 


AAGAAATCTTTGAGCCAGAAGCCCTCACTAATAATCAATCACATGGAGTTGCCACTACTGAATCTATTG


 


TAGAAGAGACAATGGCTAAGTTTGTATTTCCCAGTGCTACATACCTTCGACTTGAAAACTTGCCCAACT


 


TGAAATGTTTTTACTCGAGGACACATGCTACCGAATGGCCATCTCTGAAAAAAATGAAGGTTCTCGATT


 


GCCAAAATGTGCAGATTTTTGCTTCAGAATGTCCTGCCTTTGGAGAAACACAAGGAGCGAGCACAGA


 


AATTAATATCTCAAATCAACCTCCTCTGTTTCGGGTCAACGAGGTTACATTCCCAATCTTAGAAGAATTA


 


AAATTGAAGCCAGATGATACATGGCATGGACAAGTACTCTCAACAGAGTGTTTCAGCAAATTGAAAGT


 


TCTAGAGCTCATCTGCATTCCTGAGAAGGCAACAGATCTTGCATGTTGCTTCATTCAATCATTGCCGAA


 


TCTTGAAAAGTTACTTGTGAAGGATTCTTCTTTCTGTCAAATATTCCAGTTTGAAGGACTCAGTGATGA


 


TGACCAAAGACATGCAGCACTCACTCGGTTAAGTGAATTGAGATTGTCTAAACTTCCAGAGTTGACAC


 


ATCTCTGGACGGAAGAATTCCAACCCGGAGCAGCATTTTCTAACCTGAAACTTCTTGAAGTGCTTCATT


 


GTGTCAAATTAAAGACTTTAGTCCCATCTTTGGTGTCTTTCAACAATTTAACAACTCTGAAAGTTTCAG


 


GATGTCATGGATTAACCAATTTAGTAACATGCTCAATAGCTACAAGCTTGATGCAACTCAAAAGAATGA


 


GTATAACTGATTGCAACATGATAGAAGAGATCATAGCATGTGATGCTGATGAAATTCAAGGTGCCATTG


 


TTTTCTCCCAGTTGAGATATTTGAAACTCAGCTGTCTACCAAGTTTGGCAAGCTTTTGCTTAGGCAATC


 


AGAGCTTTGATTTCCCAACCTTGCAAAAGTTGATTGTTCATGAATGCCCAAAACTGGAGATTTTCTGTC


 


AAGGAGACTTAACCACCCCAAAGCTGCAGCAAGTGCTATTGCCAGAGTATGAATATGAATACTATGAT


 


GCAGAAGAGTATGAAGATGAATATGATGCAGAAGAGTATGAAGAAAACAGCATGTGGGAGGGCGAC


 


CTTAAAAGTACTATAAGAAAGCTGTTCGAAGAAATGGCAGAGGATGAAGATGAAGATGAAGATGAAG


 


ATGATGATGAAGAGCATGAAGATGAAGATGAAGATGAAGATGATGATGAAGAGCATGAAGATGAAGA


 


TGAAGATGAAGATGATGATGGTTGA


 


Organism Name: Corchorus olitorius


Type: PRT


Length: 2152


Sequence name: CC-NBS-LRR1 resistance protein peptide


SEQ ID NO: 10



M E F V V G I V S S I F T P A V Q L I I S P I K N K I K Y I S N H E N N V Q T L K N Q V E S L K D E R



 


K R V Q H S V D A A R Q N G E E I E D D V K K W Q K T V D Q K I A D E V E K V I A D E E K A K K


 


K C F V G L C P N L W A R Y K H S V K A E E K G K V V A K L L E Q G K F D K V S Y R P A P Q G A


 


S V T A A F V K G F E E F K S R E V L L K G I M E A L N D D K I N I M G V H G M G G V G K T M L


 


V K E V A R Q V K E G R L F D Y V V M A K V T Q T V D V K T I Q N D I A E L L G L R F D E Q S I V


 


R R A D R L R E R L K R E T K V L V V L D D V W E R L D L E E V G I P V A D E H K G C K I L L T S R


 


D L N V L S N G M N S E K N F V V G L L T E E E T W N L F K K K A G Y V V E S S D I K P T A I E V


 


A K K C A K L P I A I A T V A G A L R N K E A F H W K D A L C Q L Q K P S T V N L K G V A T T V H


 


S A I K L S Y D F L E S E E V K F T F L L C C L L G R N G L I E D L L K Y V I G M R L F Q G I T I E E T


 


R N R V L T V V S N L K A S C L L L D S Y N N E K F D I H D V V W D V A L L I A S R D R H M F V L


 


R D G E E L K D W P T Q E M K E N C S A I N F R C P R I M T E L P D E M E G L R L S L L R L D N V


 


G A L E I P A N F F R R M E R L D V L H F T R M H F S S L P V S I S L L T N L H T L C L S D C A L Q


 


D I T I V G K L K N L E I L S L A R S V I E A L P E E T A Q L T R L R L L D L S H C S K L Q L I P P N V


 


L S S L S K L E E L Y L Y N S F V Q W E G E V H S S G R R N A S L D E L K H L S H L T T L Y V H I P


 


N A E I V P K D L F I E R L E R F S I L I E D E R R W Y S E F E Y S R T L K L K I Y T S I Y L D H A V R


 


M L L K K T E D L H L Y Q L K G I K N V L D E L I D G V E L P H L K N L H I R N G S E V Q Y I M R K


 


K I E C A Q L K S M T L E G L P K L I S F W F E D K R C S T S H E E R A T S S N P L P L F N K Q L V


 


F P C L E S L R L S S I N A E R I W H S P L S E N C T F A A N L K S L T V E G C G E L E H L L S P S V


 


A R S L V Q L T H F E V A R C Q R L R E I I S T E E I E D E S V A I C F P Q L N S L E I R S L Q N L A


 


N F C A G N Y N I E F P A L K V L E V N G C P V L K E F I R V N K S E F H V P A L F N E K V A L P S


 


L E R M E F S Y L K N V K M I F D K Q L L A G S F C K L K A M S V Y H C D A L L T I F S S N I F G A


 


F Q S L E N L D V Y R C N S L E M I F E V G G L N I R E P H V V H S Q L R S L Y I S S L P A L K H V


 


W N K D P Q G I L S F Q N L H T V D L S F C R N L K S L F P V S V A K H L Q Q L E N L R L C N S A


 


V E E I V F S E E G L E E P I G F E F A Q L S S L V L Y N L R E L K C F Y R G Q H T I V W A M L K K


 


L E T D H S T L L K I V G S N S Q H L G I Q E M N S N D P P E C T T G Q P L F S T E K V I P I L E E L


 


H L R L T N P D D I S K I C D G H F L Q R F C N L E S L E L S S S E G D D A Q I L P D A V T L P R I K


 


T L I L S S C N F L K H I W E K K D S E L G H I L Q K L E I L E V N E C G D L T S F G P S S A S F Q N


 


L T T L E V T Y C N M M I N L A T P S V V Q N L V Q L T T M R I A Y C R G M A E I V A N E G G E


 


A T P T Y E I N F S K L Q S L E L N R L H R L T S F S P G N Y T I N F P S L Q E L M R V K E S H N D


 


R K G R W A G D L N T T I Q L L Y S V N V V E G Y H G I C N L K L S D T S P E L M E I W N G R N


 


P H E I V D L K F L G R V E F C N C S S L K Y I F T L S R L L S L K H L S Y L V V K E C S T L K E V V


 


M E Q E I E E E A T T D N F I F P N L R Y I K I E S C S S L R C F Y L G S G A L E I P R L E I I E I T D C


 


P K M T T F A S S F P R D E E K E I S A D G S E K R V G H G D L N I E P F F S D K V A L P S L E R L


 


R I K G M G K C R K I W Q D Q L T V N S F C E L K Y I L V E S C E K L S N I F P F N M M E R L E K


 


L E E L Q I V N C D S L E E I F E P E A L T N N Q S H G V A T T E S I V E E T M A K F V F P S A T Y L


 


R L E N L P N L K C F Y S R T H A T E W P S L K K M K V L D C Q N V Q I F A S E C P A F G E T Q G


 


A S T E I N I S N Q P P L F R V N E V T F P I L E E L K L K P D D T W H G Q V L S T E C F S K L K V L


 


E L I C I P E K A T D L A C C F I Q S L P N L E K L L V K D S S F C Q I F Q F E G L S D D D Q R H A A


 


L T R L S E L R L S K L P E L T H L W T E E F Q P G A A F S N L K L L E V L H C V K L K T L V P S L V


 


S F N N L T T L K V S G C H G L T N L V T C S I A T S L M Q L K R M S I T D C N M I E E I I A C D A


 


D E I Q G A I V F S Q L R Y L K L S C L P S L A S F C L G N Q S F D F P T L Q K L I V H E C P K L E I F


 


C Q G D L T T P K L Q Q V L L P E Y E Y E Y Y D A E E Y E D E Y D A E E Y E E N S M W E G D L K S


 


T I R K L F E E M A E D E D E D E D E D D D E E H E D E D E D E D D D E E H E D E D E D E D D D G


 


Organism Name: Corchorus olitorius


Type: DNA


Length: 928


Sequence Name: CC-NBS-LRR2 resistance protein; partial cDNA


SEQ ID NO: 11



ATGAAATGTCCTCAACTTTTGTGCCTGATGGATGAAGAGAATGAGCTGCCCTCCAATCTGGAGTATGTG



 


GAAATTGAAGATTGTAGTAACCTGGCAAAGCTTCCAAATGGGCTACAAAAGCTTCGATCATTGAAAGA


 


TTTGAGTGTTAAATGGTGCCCCAAACTGATGTCTTTTCCAAATGCAGAGTTGCCATCTACGCTGAAAAC


 


TTTGTCAATCTTGGGATGTGAATCTTTAGAGTCTTTACCCAAGGGACTGGTGCACAATGGTAGCAGCA


 


GCATTGGTAGATGTAATCTTGATAACCTGGAGATTCTAGGATGTCCATCTCTTAGATTGTTTTCAACTGG


 


TGAGCTACCAACTTGCCTTAAGCAACTCGATATTTGGGATTGCATGCAGTTGAAGTGTATTCCAGAGA


 


GATTGCTGGAGAATAGTCAGTCACTTGAATTTATTCGTATTGGGAACTGCAAAAATTTGAAAACCTTAC


 


CGCAGTGCCTATACAGGTTTGATTATCTAACTGAGTTGCATGTAAATCAATGCCCTTCCTTGGAGTCTTT


 


CCCAGAAAAGGGCTTGCCTATTCGCAACCTCAATCTGGTTTTAATATCCAACTGTGTGAATCTTAAGTC


 


CCTACCAAATCGGATGCATTACCTCACATCCCTGCAGTATTTGACTTTATTTGGTTGTCCAAGTGTAGAA


 


TCCTTTCCGGAAGAAGAGTTTACTATTCCAACAACTCTTGTCCACCTGCGAGTCCAGAGTCTACCTAAT


 


CTGGAATATTTATCTAAGGGGCTCCAGGACCTTGTTTTTCTTGAATCATTGGATGTCTGGAATTGCCCTA


 


AGCTTCAGTACTTGCCAAAGGATGGCCTGCCAAGCATGCTTGGTTTACTTCAGATCAGAAACTGTCCT


 


CTTCTAGAAAAAAAATGCTTATATGAGAAAG


 


Organism Name: Corchorus olitorius


Type: PRT


Length: 309


Sequence name: CC-NBS-LRR2 resistance protein peptide


SEQ ID NO: 12



M K C P Q L L C L M D E E N E L P S N L E Y V E I E D C S N L A K L P N G L Q K L R S L K D L S V K



 


W C P K L M S F P N A E L P S T L K T L S I L G C E S L E S L P K G L V H N G S S S I G R C N L D N


 


L E I L G C P S L R L F S T G E L P T C L K Q L D I W D C M Q L K C I P E R L L E N S Q S L E F I R I


 


G N C K N L K T L P Q C L Y R F D Y L T E L H V N Q C P S L E S F P E K G L P I R N L N L V L I S N


 


C V N L K S L P N R M H Y L T S L Q Y L T L F G C P S V E S F P E E E F T I P T T L V H L R V Q S L P


 


N L E Y L S K G L Q D L V F L E S L D V W N C P K L Q Y L P K D G L P S M L G L L Q I R N C P L L E


 


K K C L Y E K


 


Organism Name: Corchorus olitorius


Type: DNA


Length: 565


Sequence Name: CC-NBS-LRR3 resistance protein; partial cDNA


SEQ ID NO: 13



ATGCCGAGCAATTTTGGTGCATTAACTAATCTTCAACTGCTATCAGATTTTGTTGTGGGCAAAGACAAG



 


GGATATCAAATAAGGGAGCTACAGGATTTATCAAATCTCAAGGGTTCACTTTATATTTCAGGGTTAGAG


 


AATGTTGTTGAAACTGAAGATGCATCAAAGGCTAAGATACATGATAAGTCAGGACTAGATAAGTTGGT


 


GTTAGACTGGAAGAGTGGAATGAAGAAGAATAGAGACTTTGAGAATATAAGAGAAGATGTTGAGCAA


 


AAGGTGTTGGATTTGCTTGAACCATCTAAACAACTTAAAAAGCTTGTTATTATGCACTACAGAGGTTTG


 


ATGTTGGCAAAATGGGTGGGAAATTCTTCATTGACTAATTTAGAATCTTTACAGCTTATAAATTGTACCA


 


ATTGCTTGTCATTGCCATCGCTTGGGGAACTACCATTGTTGAAAAATGTGGTGATCAGGAGATTGGATA


 


GTATAAGCAGTGTGGGAGTAGAGTTCCTTGGAGAAAAAACGATGGAACCTTTTCGAGCATTGGAGCT


 


TTTACAATTTGAAGACA


 


Organism Name: Corchorus olitorius


Type: PRT


Length: 188


Sequence name: CC-NBS-LRR3 resistance protein peptide


SEQ ID NO: 14



M P S N F G A L T N L Q L L S D F V V G K D K G Y Q I R E L Q D L S N L K G S L Y I S G L E N V V



 


E T E D A S K A K I H D K S G L D K L V L D W K S G M K K N R D F E N I R E D V E Q K V L D L L E


 


P S K Q L K K L V I M H Y R G L M L A K W V G N S S L T N L E S L Q L I N C T N C L S L P S L G E L


 


P L L K N V V I R R L D S I S S V G V E F L G E K T M E P F R A L E L L Q F E D


 


Organism Name: Corchorus olitorius


Type: DNA


Length: 3141


Sequence Name: NBS resistance protein; complete cDNA


SEQ ID NO: 15



ATGGATCCATTACAAGCTGTTGCAGCTGCAACACAAATAATATCCAGTATGGTTGGGGCTGTTGGAGC



 


ATTAGAACAAGCCTCCAGAAATCTTGATGAAGCTCCAAAGAGAATCCGAAGCCTAGAAGAATTTGTGT


 


GTGATCTTGAGAATTTGGCACAACGAATTAGGCAAAAACATGCCAACAAGCTTCACAATGCTCAGTTA


 


GATTACCAACTTCAAAGTTTGCATGCCCTTATAGAAAGGCTGCGCCCAAACATCAGGAAGGCAAGAA


 


CAGTTGTATCAAAAAGTAAAATCAAGAACTTGGCTAAGGTATTCTGGAATTCCATGGCCGGAGATCCG


 


CTTGGAAAACTGACAGTTTTAATTAAAGATGACTTAAATTGGTGGCTTGATACTCAAATGTTGGCACAA


 


AATGTTGAGAAAGTACTAGAATCAACCGCACAAGATACGCCAGTTCGATTGAAAATAAAGACTGATCA


 


AGGCTACCCAACTTCTAGTAAATGTATCTTTGTGAAGGAGTTGCTTGAACAGGAGGATACTCATCGAG


 


TCATTCTAATTGTTGGGTTATCTGGTATTGGAAAGTCTTGTTTAGCTCGTCAAGTAGCTTCTGATCCACC


 


CAAAAAATTTGCAGGTGGAGCACTTGAACTTGGATTTGGGCAATGGTGTAGTCGTGCTGCTTGCAAT


 


GGCAGTAAGGTTGAATATCAGAAGCGTTTGGCAAGGAAAATTAGTAAATTCCTGGTGCAGATTGGGT


 


TCTGGAAAAAGATTAAGGAGGAGAATAGTGGAGATCTTGATTATGTTTGTTACTTGCTTCAAGAAGCC


 


TTGTATGGGAAAAGCATTTTAGTCCTTCTTGATGATGTATGGGAGCAGGACATAGTTCAGCGGTTTGCT


 


AAACTGTATGATAACAATTGTAAGTACTTAGTAACAACAAGAAATGAAGCCGTCCATGAAATTACAGAA


 


GCTGAAAAGGTAGAGCTAAGCAAGGATGATATAAGGGAGATAAGCAAGGGAATCCTTCTGTACCATA


 


GCCTCCTTAGTGAAGAAGAGCTTCCGGGTATAGCAGAGAGCTTACTTGAACGGTGTGGCCACCACCC


 


TCTAACAGTTGCTGTTATGGGAAAGGCTCTTAGAAAAGAAGTAAGAGCTGAGAAATGGGAGAAGGCT


 


ATAACCAACCTATCAACTTTTGCTACATGCGCACCAGGTCCAGTCTCATATGTGAATGAGAAAGATGCT


 


GAGGACACATTAACCATTTTTGGTTCATTTGAGTTCAGTCTAGAAGCAATGCCTGTTGACTCAAAGAG


 


ACTCTTCATCGCTCTTGCTTCTCTTTCATGGGCAGAACCGGTACCAGAAGCATGCATAGAGGCTATATG


 


GTCATGTATCGGGCAGGAGAGCTTGTTTTCACTCATTGTCTGCAAGCTTGTCGAAGGGTCTTTATTGAT


 


GAAGGTAGACATGGATCCACTATACCAAGTACATGACATGGTTTCATTGTACCTTGATAGCAAGACTAC


 


TGATTCAATTGAGATGCTACTGCATAGATCTAAACCAGAAGAAACTGCATTTATTTGCCCTTGGCTTCTT


 


ATTTTTGGGAAAGAGAATGTGAAAAAGATTGTTGAAGAGAGGATGAAGCTTTTCTTTGATATTTTAGA


 


TGAAAAACAAGTGGTTATCACCTTAGAATCCAGTATTGAGGCTCTAATGGCAAGCAAATCCATATCTGA


 


ACTCGAAGCAAGCAGAGCAAGCTTTAGTAGGATATTGGGACCCAAGATTACGGATATTGTCTCAACTA


 


ATTCACAGAGTATGATTGCAGTGTCTGCAGAAGCATCATAATCATTTTTATAAGACTGATTATTGCAA


 


CTATTTTCCATCCCTTGAAACTGACAGTACAGTTGATAAGTTGGCAAGTATGTTAGAAGATTGCGAAGA


 


TCCTGTAATCCAAACAAACATTTTAACCATCCTTGCCAAGATTGCTGAGTTTGGAAGCCCGGAGATTGT


 


TGATAAGGTGCTTCAAAGTATCCCCTTTAACCAGGTTGCTGACTTGCTCTCTCCCAATGCCAAGGATTG


 


GCATGAGAGCATGTTTACAATATTGATGTCTTTGACCAAAGCTGGAAAGTCAAAAGCTGTTGAAAGAA


 


TGTTTGCTTTTCAGATTGATAAAAATCTGATTAACCTTATAGAGAGTGAATCTGAACTAGTGCAACACCA


 


TGCCATTGTCACTTTGAAGGCATTTTATGAGCTGGCTGGCCCTTCTTTGAATAGTTCTCTTCGACCTGCT


 


AATCTAGACCTCTTGCCATGGCAAGTGAGACTTCGTTTAGAGAGATTTGTTATGCCAGACCGGAACAT


 


TCCCCTTTCCCCGAAACCACAAACTTTTGAAGATCTTATCCACAAGATGCTAGATAATGACAACAAACA


 


GGTGTTGGAGGCTATGCAGGATCTTGTGCCGATAATTGAAAAGGCTGGAGACCCAGGTTTCAGACAG


 


ATGATTGTTCAAAGTCCCCTAATTAGAAGGTTATCAGAACTTCTGCAACATGGACATACCGAACAAAAT


 


TCTATAAGATCAGAATCTGCATTTTTACTAATGAAGCTAGCTTACTCTGGTGGGGAACCCTGCATCAATA


 


AGTTTCTAGAGTTTGATGTTATTCCTGAGCTGGTAAAGATGATGCAGTGCAACACTGCAGAGTTGCAG


 


GATTCAGCCTATACAGCTCTGCACCAAATGCTTTTTGGCAATGGTGGGGTTCTTGTTTTGAGTAAGATC


 


TTCAAAATGGGTCTAATAGACAAGATTCCTTATGCACTTGAGAGCAAATCTGCGAAGACTCGGGAAGT


 


CCTGCTGCATTTTGTATTTGATATTGTTGAGCTGGGAAGCAAAGCCTGCTTAGAGAAAATGCTATCTTT


 


GCAAGTTGTGGAGAAACTCACCAAGTTAGAAAAAAGTGGTGGGGGCTCTGGTGAAATTGTGATTGG


 


ATTTCTGAAGGCGATGGATAAGTGTAAGCATCTCACAGTAGCGGAGCGAAAGGTGATGAAACAACAG


 


GTGGTTAGAAAGGTAAGAGCCTCCTTGAAAGGCCACAAATTCGAAACTCGGATTTTAGCGGCTGTAG


 


AAGCTTTCCTCTCTGGAGGGTCAAGGGGTGCAAGTGGTAGTGGCAGTGGTCGGAATAGGAAGTAA


 


Organism Name: Corchorus olitorius


Type: PRT


Length: 1046


Sequence name: NBS resistance protein peptide


SEQ ID NO: 16



M D P L Q A V A A A T Q I I S S M V G A V G A L E Q A S R N L D E A P K R I R S L E E F V C D L E



 


N L A Q R I R Q K H A N K L H N A Q L D Y Q L Q S L H A L I E R L R P N I R K A R T V V S K S K I K


 


N L A K V F W N S M A G D P L G K L T V L I K D D L N W W L D T Q M L A Q N V E K V L E S T A


 


Q D T P V R L K I K T D Q G Y P T S S K C I F V K E L L E Q E D T H R V I L I V G L S G I G K S C L A


 


R Q V A S D P P K K F A G G A L E L G F G Q W C S R A A C N G S K V E Y Q K R L A R K I S K F L V


 


Q I G F W K K I K E E N S G D L D Y V C Y L L Q E A L Y G K S I L V L L D D V W E Q D I V Q R F A


 


K L Y D N N C K Y L V T T R N E A V H E I T E A E K V E L S K D D I R E I S K G I L L Y H S L L S E E E


 


L P G I A E S L L E R C G H H P L T V A V M G K A L R K E V R A E K W E K A I T N L S T F A T C A


 


P G P V S Y V N E K D A E D T L T I F G S F E F S L E A M P V D S K R L F I A L A S L S W A E P V P


 


E A C I E A I W S C I G Q E S L F S L I V C K L V E G S L L M K V D M D P L Y Q V H D M V S L Y L


 


D S K T T D S I E M L L H R S K P E E T A F I C P W L L I F G K E N V K K I V E E R M K L F F D I L D


 


E K Q V V I T L E S S I E A L M A S K S I S E L E A S R A S F S R I L G P K I T D I V S T N S Q S M I A


 


V S A E A I I I I F S K T D Y C N Y F P S L E T D S T V D K L A S M L E D C E D P V I Q T N I L T I L A


 


K I A E F G S P E I V D K V L Q S I P F N Q V A D L L S P N A K D W H E S M F T I L M S L T K A G


 


K S K A V E R M F A F Q I D K N L I N L I E S E S E L V Q H H A I V T L K A F Y E L A G P S L N S S L


 


R P A N L D L L P W Q V R L R L E R F V M P D R N I P L S P K P Q T F E D L I H K M L D N D N K


 


Q V L E A M Q D L V P I I E K A G D P G F R Q M I V Q S P L I R R L S E L L Q H G H T E Q N S I R


 


S E S A F L L M K L A Y S G G E P C I N K F L E F D V I P E L V K M M Q C N T A E L Q D S A Y T A


 


L H Q M L F G N G G V L V L S K I F K M G L I D K I P Y A L E S K S A K T R E V L L H F V F D I V E


 


L G S K A C L E K M L S L Q V V E K L T K L E K S G G G S G E I V I G F L K A M D K C K H L T V A E


 


R K V M K Q Q V V R K V R A S L K G H K F E T R I L A A V E A F L S G G S R G A S G S G S G R N


 


R K


 


Organism Name: Corchorus olitorius


Type: DNA


Length: 3009


Sequence Name: Leucine-rich repeat trans-membrane protein kinase (LRR-TM-PK);


complete cDNA


SEQ ID NO: 17



ATGGGAAGAGCTGATTTTACAACTACTACTCCAAAGCTGCTACTTCTACTTGTTTTCATTGCAATGCTCT



 


GGCTTTCAACCTTTGGATTTGCTGCTGCTACTCCTCTTCTTCATTCTGAAGAAGTGAAGGCACTGAAA


 


GCAATAGGGAAGAAGATGGGGAAGAAGGATTGGGATTTTGGGGTGGATCCTTGCAGTGGAAAGGG


 


GAAGTGGATTGAGGGAGATGAAGAAACTGGATTTGCAAGCAAGGTCACCTGCAATTGCTCTTTCAAC


 


AACAACACCACCTGCCATGTAGTAACCATGGACCTAAGTCGCAACTACTTCACAGGTTCTATTCCTAAA


 


GAATGGGCTACCATGAAGTTGGACATGCTCTCTTTCATGGGGAACCGGTTGTCTGGTCCATTCCCAAA


 


AGTTCTTACCAACATCACAAGCCTCACAAACTTGTCTATTGAAGGGAACAACTTTTCAGGACCCATTCC


 


TCCAGAGATTGGAAAGTTGATCAATTTACAGAAACTTGTTCTCTCATCTAATGCCTTAAGTGGAGAATT


 


GCCTGCAGAACTAGCCAAGTTGGTCAACTTGACTGATATACGGTTTAGCGACAATAACTTCTCTGGAA


 


AGATACCTGATTTTATCAGTAACTGGAAGCAGATTCAAAAACTGCAGTTTCAAGGTTGCTCTCTTGAG


 


GGGCCTATACCTTCCAGCATTTCTACTTTGACTAGCTTATCTGATTTGAGGATTAGTGACTTGAAAGGC


 


AAAGGGTCCCCATTTCCGCTATTGCGTAACCATGACTCACTGAAGACATTGATACTAAGGAACTGCAA


 


GATACATGGAGAAATCCCAGAGTATATTGGGGATATGAAGAAATTGAAAACCCTGGATCTCAGCTATAA


 


TAACTTGACTGGAGAAATTCCCAGTTCATTCTACAAACTGACAAAAGCTGATTTCTTGTATTTGACTCG


 


GAATCAGCTCACCGGGTCTGTCCCTGAGTGGATCCTAGAAAGAAATAAAAATGCGGATATATCTTTCAA


 


CAATTTCACCTGGGACACATCAAGTCCAATAGAATGTCCTCGAGGGAGCGTGAACTTGGTTGAGAGCT


 


ACTCCACACCAACAAATAAACTAAGTAAAGTTCATTCATGTCTAAAACAGAATTTCCCATGTTCAGCTTC


 


AACTAGTCAACATAAATACTCCTTGCACATAAATTGTGGGGGGCAGGAATTAAATGTCAATGGTGATGC


 


CAAATATGAAGCTGATATGGAACCAAGAGGTGCTTCCATGTTTTACCTGGGGCACAACTGGGCATTAA


 


GCAGCACTGGAAACTTCATGGATAACGATATTGATGCAGATGACTATATTGTTACCAATACTTCTGCATT


 


GTCCAATGTATCTGCAACTCATTCCGAACTCTACACAACTGCGCGTGTTTCTCCCCTCTCTCTCACATACT


 


ATGGACTCTGCCTAGGGAATGGGAACTACACTGTTAATCTTCATTTTGCAGAGATTATTTATATAAATGA


 


TCGATCATTTTACAGCCTTGGGAAACGCATATTCGATGTCTATATTCAGGGAGAATTGGTGCTGAAAGA


 


CTTTAATATTCAAGATGAAGCTGGAGGTACTGGTAAGCCCATTGTAAAGAACTTTACAGCCGTCGTGA


 


CAAGAAATACATTAAAAATCCACTTATACTGGGCTGGAAGGGGAACAACAGGCATACCAGCAAGGGG


 


GATGTATGGTCCACTCATATCAGCTATATCAGTGGTTTCAAACTTTGAGCCCCCAACTGTTGTTGGCAA


 


GAAGAATTATCTCATAATTGCGGCAGGGGCAGCTTCTGCAGCAATACTTATAGTCCTTATGGTCTTAGG


 


TATCATTTGGAGAAAAGGCTGGCTGGGAGGCAAAATCTCTGCTGAAAACGAGCTGAAAGACCTGGAT


 


CTGCAAACAGGAATTTTCAGTCTAAGGCAGATTAAAGCTGCCACCAACAACTTCGATGCTGAGAACA


 


AAATTGGTGAGGGTGGATTTGGTTCTGTTTACAAGGGTTTATTATCAGATGGGACAGTTATCGCAGTG


 


AAGCAGCTTTCATCAAAATCTAAGCAGGGAAATCGTGAATTTGTGAATGAAATAGGCATGATATCTGCA


 


CTGCAGCATCCGAATCTTGTGAAGCTCTATGGATGTTGTGTAGAAGGAAACCAGTTATTGCTAGTTTAT


 


GAGTACATGGAACATAACTGTGTATCTCGTGCTCTTTTTGGGAAGGGCTCAACACCCAAATTGAAACT


 


GGACTGGTCTACCCGGAAAAACATTTGCCTTGGTATTGCCAGGGGTTTGGCCTACCTCCATGAAGAGT


 


CGAGAATTAAAATTGTGCACAGGGATATTAAAACAAGTAATGTGTTGCTTGACAAGAATCTAAATGCAA


 


AAATTTCTGATTTTGGTTTGGCAAAGCTAAATGATGATGACAAAACCCACATCAGCACTCGTATAGCCG


 


GGACAATTGGTTATATGGCTCCTGAGTATGCAATGCGTGGATACCTAACCAGCAAAGCTGATGTCTATA


 


GCTTTGGTGTTGTTGCATTGGAAATTGTTAGTGGAAAGAGCAACACAAACTACAGACCAACTGAGGA


 


CTTTGTTTACCTTCTTGACTGGGCCTATGTTTTGAGAGAGAGGGGAAGTTTGTTGGAGTTGGTTGATC


 


CAGAGTTGGGATCAGAGTACTCATCAGAGGAGGCAATGGTGATGCTGAATGTGGCTCTACTATGCACC


 


AATGCAGCCCCCACCCTGAGGCCTACCATGTCACAGGTTGTGAGCATGCTCGAAGGCCAAACCTCAG


 


TTCAAGACATCCTCTCCGACCCTGGATTTTCATCCATGAATTCGAAATTCAAGGCCTTAGTTAATCACTT


 


CTGGCAAAATCCAAGCCAAACAATGAGTTGTCAAGCAATGGCCCAAATACAGATTCCTCAAGTTCAA


 


ACATAGAAGACATAGAAGAGAATAGTCATCTTTTGAGAGTTAGTTCTATTCAATCCGAGGCGTGA


 


Organism Name: Corchorus olitorius


Type: PRT


Length: 1002


Sequence name: Leucine-rich repeat trans-membrane protein kinase (LRR-TM-PK) peptide


SEQ ID NO: 18



M G R A D F T T T T P K L L L L L V F I A M L W L S T F G F A A A T P L L H S E E V K A L K A I G K



 


K M G K K D W D F G V D P C S G K G K W I E G D E E T G F A S K V T C N C S F N N N T T C H V


 


V T M D L S R N Y F T G S I P K E W A T M K L D M L S F M G N R L S G P F P K V L T N I T S L T


 


N L S I E G N N F S G P I P P E I G K L I N L Q K L V L S S N A L S G E L P A E L A K L V N L T D I R F


 


S D N N F S G K I P D F I S N W K Q I Q K L Q F Q G C S L E G P I P S S I S T L T S L S D L R I S D L


 


K G K G S P F P L L R N H D S L K T L I L R N C K I H G E I P E Y I G D M K K L K T L D L S Y N N L T


 


G E I P S S F Y K L T K A D F L Y L T R N Q L T G S V P E W I L E R N K N A D I S F N N F T W D T S


 


S P I E C P R G S V N L V E S Y S T P T N K L S K V H S C L K Q N F P C S A S T S Q H K Y S L H I N


 


C G G Q E L N V N G D A K Y E A D M E P R G A S M F Y L G H N W A L S S T G N F M D N D I D


 


A D D Y I V T N T S A L S N V S A T H S E L Y T T A R V S P L S L T Y Y G L C L G N G N Y T V N L H


 


F A E I I Y I N D R S F Y S L G K R I F D V Y I Q G E L V L K D F N I Q D E A G G T G K P I V K N F T


 


A V V T R N T L K I H L Y W A G R G T T G I P A R G M Y G P L I S A I S V V S N F E P P T V V G K


 


K N Y L I I A A G A A S A A I L I V L M V L G I I W R K G W L G G K I S A E N E L K D L D L Q T G I


 


F S L R Q I K A A T N N F D A E N K I G E G G F G S V Y K G L L S D G T V I A V K Q L S S K S K Q G


 


N R E F V N E I G M I S A L Q H P N L V K L Y G C C V E G N Q L L L V Y E Y M E H N C V S R A L F


 


G K G S T P K L K L D W S T R K N I C L G I A R G L A Y L H E E S R I K I V H R D I K T S N V L L D K


 


N L N A K I S D F G L A K L N D D D K T H I S T R I A G T I G Y M A P E Y A M R G Y L T S K A D V


 


Y S F G V V A L E I V S G K S N T N Y R P T E D F V Y L L D W A Y V L R E R G S L L E L V D P E L G


 


S E Y S S E E A M V M L N V A L L C T N A A P T L R P T M S Q V V S M L E G Q T S V Q D I L S D


 


P G F S S M N S K F K A L V N H F W Q N P S Q T M S L S S N G P N T D S S S S N I E D I E E N S


 


H L L R V S S I Q S E A


 


Organism Name: Corchorus olitorius


Type: DNA


Length: 1989


Sequence Name: Leucine-Rich Repeat containing protein 1 (LRR1); partial cDNA


SEQ ID NO: 19



AGAATTTGTTGTCAGTAAGATCCAACAGTCCATTGTGGCCAGGTTGGGATTGCCATGGGAAGAAAAT



 


GATTCCTATGAGCTACAAACTTCAAAGATTCACAATGTTTTAAAAAATAAGAGGTTCCTCCTATTGCTG


 


GATGATGTTTGGGAAGGAATTGATCTTAGTGAAATTGGGATTCCTCTTCCTGATGAGGAAAATAAATGC


 


AAGCTGATATTTACAACGCGGTCCATGGATGTTTGCACTGACATGGATGCTCATAGGAAGCTCAAAGT


 


AGAATTTCTGGACGAGGAGAAATCATGGCGATTATTCTGTGAGAAGGTTGGAAGAATGGAGATTTTA


 


GAGTCACCACCTGTTAGAACTTATGCTGAGACCATTGTCAGGAAATGTGGAGGTCTACCGCTTGCCTT


 


AATCACTGTGGGGAGAGCCATGGCCAATAAGGAGACTGAAGAAGAATGGAAATATGCAATTGAATTA


 


CTCAACAAATCTCCTTCTGAACTTAGAGGTATGGAAGATGTCTTTACCCTTTTAAAGTTTAGCTATGACA


 


ATTTGGATAGTGAAACAACAAAAATGTGCTTTTTGTATTGTTCTCTTTTCCCGGCAAGCTGTTCCATTG


 


AGAAACAACAGCTTGTAGAGTATTGGATTGGTGAAGGATTCCTAGACAGTTCTAATGCTCATAATAAA


 


GGGTTTGCTGCAATTGGGTCCCTAAAAGTAGCTTGTTTATTGGAAACAGGCGATGAGGAAACCCAAG


 


TAAAGATGAATGATGTTATCCGAAGTTTTGCCTTATGGATAGCATCTGAATCTGGGGTAAATAAGGGAA


 


ACCTTTTGGTAGAAGCAAGCTTGGGCCTTATTGAAGCTCCTGGAGTTGAAAACTGGGAAGAAGCAAA


 


AAGGATTTCCTTGTTAGACAATGGAATCACAGTACTAGAACAAGTACCAATATGCCCAAATCTGTTGAC


 


TCTGTTGCTTCAGTGGAATAATGGTCTGAATCGAATAGCAGCTAACTTTTTTCAATCTATGCCTGCTCTT


 


AGAGTCTTGGATTTGTCATTTACAAGCATTAGAAAGATCCCAGTAAGCATCAGTCAATTAGTAGAGCTT


 


CGGCATCTTAATTTAGCAGGTACAAAAATAACAACATTGCCTAGGGAGCTAGCAAGTTTAGCAAAGCT


 


GAATTACTTGAATCTCTCACGCACATATTCTCTTCGAACGGTTCCACGTGAGGCTTTATCTGGACTTTCA


 


GAGTTAGTGGTCTTGAATTTGTATTACAGTTATGAGGTTCGCGAAGTTCGGAATTTTGAAGGTGAAGG


 


TGAAGTTGAATTTGAGGTATTGGAGACCTTGACACAACTCCGTATCCTTGGTTTAACAATCTCCTCAAT


 


AGCCTCCCTGAATAGACTCTTTGGTTTGAGAAATCTGGTTAGATGCATACACTATTTACTCCTAAAGGA


 


GTGTGAAGGTTTAACAGAATTAGTATTTTCATCAGCTTCTGGCTTAACATTGAGAAGACTTAGCATCAC


 


AGACTGCTATGATTTCAATTACTTAGTAGTGAATGCTGAGGTGGACAAAAGTGGTTGCCTAATTTGGA


 


GGTTTTATCTTTGCATGGTCTACCAAAAGTGACTTCAGTGTGGAAATCCCCAGTAAGAAAAGCAAGCC


 


TGCAAAATTTGCGCTTGTTGAATATCTGGTATTGTCATAGCTTGAAGAATGTTTCTTGGGTATTACTACT


 


TCCAAAGTTAGAAGCAATTTACTTGTTCTATTGCAAGAAAATGGAACAAGTTGTAAGTGGAGAAGAG


 


GGACTACTAGAGCCTGATCCAAAGGCATTTTCAAGGCTTAAAACTATAGAGATCCGCGACCTTCCTGA


 


ATTAAAGAGTATCAGTCCATGGAATTGGCTTTCCCCTGCTTGAAGAGCATTGCTGTGATTGATTGTCC


 


AAAGCTGAAGAAACTACCAATTGGAACCCATAATTCCTCAACTCTTCCAACTGTGTACTGTAGTGAAGA


 


ATG


 


Organism Name: Corchorus olitorius


Type: PRT


Length: 662


Sequence name: Leucine-Rich Repeat containing protein 1 (LRR1) peptide


SEQ ID NO: 20



E F V V S K I Q Q S I V A R L G L P W E E N D S Y E L Q T S K I H N V L K N K R F L L L L D D V W E



 


G I D L S E I G I P L P D E E N K C K L I F T T R S M D V C T D M D A H R K L K V E F L D E E K S W


 


R L F C E K V G R M E I L E S P P V R T Y A E T I V R K C G G L P L A L I T V G R A M A N K E T E E


 


E W K Y A I E L L N K S P S E L R G M E D V F T L L K F S Y D N L D S E T T K M C F L Y C S L F P A


 


S C S I E K Q Q L V E Y W I G E G F L D S S N A H N K G F A A I G S L K V A C L L E T G D E E T Q


 


V K M N D V I R S F A L W I A S E S G V N K G N L L V E A S L G L I E A P G V E N W E E A K R I S


 


L L D N G I T V L E Q V P I C P N L L T L L L Q W N N G L N R I A A N F F Q S M P A L R V L D L S F


 


T S I R K I P V S I S Q L V E L R H L N L A G T K I T T L P R E L A S L A K L N Y L N L S R T Y S L R T


 


V P R E A L S G L S E L V V L N L Y Y S Y E V R E V R N F E G E G E V E F E V L E T L T Q L R I L G L


 


T I S S I A S L N R L F G L R N L V R C I H Y L L L K E C E G L T E L V F S S A S G L T L R R L S I T D


 


C Y D F N Y L V V N A E D G Q K W L P N L E V L S L H G L P K V T S V W K S P V R K A S L Q N L


 


R L L N I W Y C H S L K N V S W V L L L P K L E A I Y L F Y C K K M E Q V V S G E E G L L E P D P K


 


A F S R L K T I E I R D L P E L K S I S P W T L A F P C L K S I A V I D C P K L K K L P I G T H N S S T


 


L P T V Y C S E E


 


Organism Name: Corchorus olitorius


Type: DNA


Length: 1258


Sequence Name: CCH-type integrase; partial cDNA


SEQ ID NO: 21



AGATTTATACCAATTAGTGGAAATGAAAATACAGATTGGCCAACGGCGGATTATGAGTGGATTAAGCCT



 


TGCAGTTTAGAAGAATGCAAAACTCAATGCTTGCAAGATTGTCTTTGTACTGTGGCAGTGTTCAATGA


 


GAATGGCTGTTGGAAGAAGGCGCTACCGCTGCCATTTGGTAGGCAAGACCCAGATGTGAAATCCAAC


 


TCCTACCTGAAAGTAAGAAAACCAGAATTTTCCCAGAAAAACCCTCTTCCATTTCTAGACACTAAAAAG


 


AACCAAAATTCATTGGTAATTCTGGTTTCAGTTCTCTTGGGTAGCTCTGTGTTTGTCAACTTTATATTGG


 


TTGGGGTTTTATGTTCGGGATCGTTTTTCCTGTATCAGAAAAAGATTGCAAGAAATGGGAGAAAGTAC


 


AAGAATGGGATACAGAACAATTTGAGGTGTTTTAGTTACAAGGAGCTTGAAGAAGCTACAAATGGTT


 


TCAAGGAAGAGCTAGGAAGGGGAGCATTCGGCATAGTTTATAAAGGGCTAATAAAAACAGATGCTCA


 


AGATCCAACTGAAGCTGCAGTTAAGAAGTTAGACAGAGTGGTTCAAGACAAAGACAACGAATTCAG


 


AACTGAAGTGAGTGTGATTGCTCAAACACATCATAGGAATCTGGTCAAGTTGCTTGGATATTGTGACG


 


AAGGTCAGTGTCGGATGCTGGTGTATGAATACTTAAGCAATAAAACATTAGCAAGCTTTCTTTTTGGG


 


GATCAAAAACCCAGTTGGAACCAAAGGAAACAGATTGCTTTGGGAATTGCAAGAGGATTGCTTTACT


 


TGCATGAGGAATGCAGCCCTCAAATAATCCATTGTGATATAAAGCCTCAAAACATACTTCTTGATGATTA


 


CTATGAAGCTCGGATATCTGACTTTGGGTTGTCAAAGCTTCTAGGGACTGACCAATCATATACTAATACC


 


GCCATAAGGGGAACGAAAGGGTATGTCGCGCCAGAATGGTTCAAGACAGTGCCTGTATCCGTGAAGG


 


TTGATGTGTATAGCTTTGGTGTCCTGCTGCTAGAAATCATTTGTTGTAGAAGAAATGTAGACATGGATAT


 


TGGCAAAGCGAAAATGGAAATTTTGACAGATTGGGCTTGTGATTGCTTCCTGGAAGGCACTTTAGAT


 


GCTCTTGTTGACAATGATGCAGACGCCTTAAGCGACAAGGCGAAGCTTGAGACCTTTGTTATGGTTGC


 


CATTTGGTGCATTCAAGAAGACTTGTCTCTCA


 


Organism Name: Corchorus olitorius


Type: PRT


Length: 419


Sequence name: CCH-type integrase peptide


SEQ ID NO: 22



R F I P I S G N E N T D W P T A D Y E W I K P C S L E E C K T Q C L Q D C L C T V A V F N E N G C



 


W K K A L P L P F G R Q D P D V K S N S Y L K V R K P E F S Q K N P L P F L D T K K N Q N S L V I


 


L V S V L L G S S V F V N F I L V G V L C S G S F F L Y Q K K I A R N G R K Y K N G I Q N N L R C F


 


S Y K E L E E A T N G F K E E L G R G A F G I V Y K G L I K T D A Q D P T E A A V K K L D R V V Q


 


D K D N E F R T E V S V I A Q T H H R N L V K L L G Y C D E G Q C R M L V Y E Y L S N K T L A S F


 


L F G D Q K P S W N Q R K Q I A L G I A R G L L Y L H E E C S P Q I I H C D I K P Q N I L L D D Y Y


 


E A R I S D F G L S K L L G T D Q S Y T N T A I R G T K G Y V A P E W F K T V P V S V K V D V Y S F


 


G V L L L E I I C C R R N V D M D I G K A K M E I L T D W A C D C F L E G T L D A L V D N D A D A


 


L S D K A K L E T F V M V A I W C I Q E D L S L


 


Organism Name: Corchorus olitorius


Type: DNA


Length: 2362


Sequence Name: TIR-NBS resistance protein; partial cDNA


SEQ ID NO: 23



ATAGGATTGCATGGTTGCAAACCTTCGTTACTGAGGTTTGCAAATTGGCTCCGTGCTGAGTTGGAAGT



 


TCAAGGGATGAGTTGCTTTGTATCTGATAGAGCTCGGTTCAGGAACTCTCGTAAGCATGGAATTATTGA


 


AAGGGCAATGGATGTTTCTTCATTCGGTGTTGTAATCCTAACGAGGAAGTCTTTCAGGAATCCATATAC


 


TATTGAGGAGCTCAGATTTTTCTCGAGCAAGAAAAATTTGGTCCCGATCTATTTTGATCTGCGTCCTGG


 


TGATTGCCTTGTCCGGGATATTGTTGAGAAGAGAGGAGACTTGTGGGAAAAACATGGGGGTGAATTA


 


TGGGTTTTCTATGGAGGATTGGAGAAGGAGTGGAAGGAAGCTGTTAGTGGCCTTTTCCGAGTGGATG


 


AATGGAAACTGGAAGCTCAGGATGGTAATTGGAGAGACTGCATATTAAGGGTTGTTACCATTTTGGCA


 


ATGAAGTTAGGAAGAAGAAGTGTTGTAGAGCGATTGACAAAGTGGAAAGAAAAGGTAGACAAAGA


 


GGAATTCCCATTTCCTCGAAATGAAAATTTTATTGGCCGAAAGAAAGAATTGTCAGAGCTAGAATTTAT


 


ACTTTTTGGTGATATTAGTGGAGAATCAGAAAGAGATTATTTTGAGCTCAAGGCTAGGTCAAAGAGAA


 


GAAATTTGACAATTGGGTGGAGTAAGAGCACTTCAGTGGAGGAAAGACGTAGGGAAAGACAGCGG


 


GAGGATGGCAGCCGGAAGGGCAAAGAACCTGTGGTTTGGAAGGAGTCAGAAAAGGAGATTGAGAT


 


GCAAAGCACAGAAAGACAGCACTATCAAAGACCAAGAGGTGGACGGAATTCACAGAGAAAGAGATC


 


AGCGAAAGTTGTCTATGGGAAGGGCATTGCCTGTGTAACAGGGGAATCAGGACTGGGGAAAACTGA


 


GCTTCTTCTGGAGTTTGCCTATAAATATCACCAGAGGTATAAGATGGTCCTGTGGATAGGAGGGGAAA


 


GCAGGTATATTAGACAGAATTATCTAAACCTCTGGTCATTTTTAGAAGTTGACGTAGGGGTTGAAAATT


 


GCACAGATAAAAGCAGGATGAAAAGCTTTGAAGAGCAGGAAGAGGCTGCCATTTCTAGAGTCCGGA


 


AAGAGCTAATGAGAAACATTCCTTTTCTGGTGGTGATTGATAATTTAGAGAGTGAAAAGGATTGGTGG


 


GATCGCAAGCTTGTAATGGATCTTCTTCCCCGTTTTGGCGGAGAGACCCACATTCTGATAGCTACGCGC


 


CTTCCCCGTGTGATGAATTTAGAACCTTTGAAACTGTCATACTTGTCTGGAGTAGAGGCAATGTCATTA


 


ATGCAGGGTAGCGGCAAAGACTACCCTATTGCTGAGATTGATGCGCTTAGGGTCATTGAGGAGAAAG


 


TTGGAAGGCTGACTTTGGGCCTTGCTATAGTAGGCGCAATTCTATCTGAACTACCCATAAATCCAAGCA


 


GGCTATTGGATACCATCAACAGAATGCCCTTGAGAGACTTTTCATGGAGTGGTAAGGAAGCTTATTCG


 


CTGAGGAAAAACAGTTTCCTCCTGCAACTCTTTGAGGTATGTTTTTCAATATTTGATCATGCAGAGGGA


 


CCAAGGAGCTTAGCAACGAGAATGGTCCAGGTGAGTGGTTGGTTTGCACCTGCTGCCATTCCTGTTT


 


CCCTGTTAGCCATGGCTGCTCACAAGATACCTGAGAAACATAAAAGGACCCGCTTTTGGAGAAGATTA


 


TTGCGCTCCTTAACTTGTGGGCTTTCTTCATCATACTCCAAGAGGTCTGAAACAGAAGCATCTTCAATG


 


TTGTTGAGATTTAATATTGCACGAAGCAGTACCAAGCAAGGTTATGTTCATTTTAATGAGCTCATCAAG


 


GTTTACTCTCGGAAGAGAGGAGTTGCTGGAGTTGCACATGCCATGGTCCAAGCTGTTGTTAGTCGTG


 


GATCAATTTTAGATCACTCAGAACATATGTGGGCAGCATGCTTTTTGCTATTTGGATTTGGTAATGATCC


 


TACAGCTGTTGAGCTCAAGGTGTCGGACCTATTATACCTTGTCAAAGAGGTGGTTTTGCCTCTTGCAAT


 


TCGGACATTCATTACATTCTCTAGGTGCAGTGCTGCTCTTGAACTCCTCCGGCTCTGTACCAATGCTTTG


 


GAGGCAGCAGATCAAGCATTTGTTACACCAGTTGAAAAATGGTTGGACAAATCACTTTGTTGGAGGC


 


CCATTCAAACTAATGCTCAGTTAAATCCATATCTTTGGCAGGAGTTGGCATTATCAAGAGCTACTGTGCT


 


AGAAACCAGGGCCAAGTTGATGCTAAGAGGGGGGCAATTTGACATAGGAGATG


 


Organism Name: Corchorus olitorius


Type: PRT


Length: 787


Sequence name: TIR-NBS resistance protein peptide


SEQ ID NO: 24



I G L H G C K P S L L R F A N W L R A E L E V Q G M S C F V S D R A R F R N S R K H G I I E R A M



 


D V S S F G V V I L T R K S F R N P Y T I E E L R F F S S K K N L V P I Y F D L R P G D C L V R D I V


 


E K R G D L W E K H G G E L W V F Y G G L E K E W K E A V S G L F R V D E W K L E A Q D G N


 


W R D C I L R V V T I L A M K L G R R S V V E R L T K W K E K V D K E E F P F P R N E N F I G R K


 


K E L S E L E F I L F G D I S G E S E R D Y F E L K A R S K R R N L T I G W S K S T S V E E R R R E R


 


Q R E D G S R K G K E P V V W K E S E K E I E M Q S T E R Q H Y Q R P R G G R N S Q R K R S A K


 


V V Y G K G I A C V T G E S G L G K T E L L L E F A Y K Y H Q R Y K M V L W I G G E S R Y I R Q N


 


Y L N L W S F L E V D V G V E N C T D K S R M K S F E E Q E E A A I S R V R K E L M R N I P F L V


 


V I D N L E S E K D W W D R K L V M D L L P R F G G E T H I L I A T R L P R V M N L E P L K L S Y


 


L S G V E A M S L M Q G S G K D Y P I A E I D A L R V I E E K V G R L T L G L A I V G A I L S E L P I


 


N P S R L L D T I N R M P L R D F S W S G K E A Y S L R K N S F L L Q L F E V C F S I F D H A E G P


 


R S L A T R M V Q V S G W F A P A A I P V S L L A M A A H K I P E K H K R T R F W R R L L R S L T


 


C G L S S S Y S K R S E T E A S S M L L R F N I A R S S T K Q G Y V H F N E L I K V Y S R K R G V A


 


G V A H A M V Q A V V S R G S I L D H S E H M W A A C F L L F G F G N D P T A V E L K V S D L L


 


Y L V K E V V L P L A I R T F I T F S R C S A A L E L L R L C T N A L E A A D Q A F V T P V E K W L D


 


K S L C W R P I Q T N A Q L N P Y L W Q E L A L S R A T V L E T R A K L M L R G G Q F D I G D


 


Organism Name: Corchorus olitorius


Type: DNA


Length: 2565


Sequence Name: Resistance gene analog 1 (RGA1); complete cDNA


SEQ ID NO: 25



ATGGCGGATGCAATAGTCAATGTATTCTTGGAAAAGCTGTTAAGCACTCTTGCAGAGGAAGGCCGCTA



 


TGTTACTGAATTCAGGGATCAGTTTGAGAAACTACAAACTGAGCTTCAATTGCTGCAATGCTTCCTCAA


 


GGATGCAGATAGGCTGAAGAGGAAAAATCACACTATACGAAAAATCTTGGCTGATCTTCGCGAGTTAA


 


TATATGAAGCTGAAGATATCCTAGCAGATTGCCAGCTTCAGTCAAGGGATGAAAACCAATTTTCCCAAA


 


GCTGGTTAGCTTGTTTCTCTCCACCAAAGCTTCATTTCAAGTACCAAAGTGGGAAGCGTCTTAAGGAA


 


ATTACTGAGAAAATCACAAGCATTAAACAAAACATCTCATCATTCCTTGGAGGGCCTCTTTTATTCCAAC


 


CAGAGGTCATAAGCGCGCAGGACCAAATGCCTAGATGGAGTTCGCAGGTGTATGATCACACTCAAGT


 


GGTTGGACTAGAATCAGACACTCAAAAGATGAAAGACTGGATATTTGATGCAGTTCATGAAGGAGCT


 


CAGGAAATACTAGCAATTGGAGTTGTTGGTATGGGTGGGCTTGGAAAGACTACCATTGCTCAAAAGG


 


TTTTTAATGAAAGAGATATAGAACATCACTTTGACAGAAGAATGTGGGTTTCTGTTTCTCAAACATTCA


 


CTGAAGAACAAATTATGAGAAGTATGTTGAGGAACTTGGGAGATGCAAGTGTGGGAGATGATAGAAA


 


TGAACTGCTGAAGAAAATTAACCAATATCTCTTAGGAAAGAGGTATTTGATTGTGATGGATGATGTGTG


 


GAGTGAAGATGTTCTGTGGTGGCAACGAATCTGTGAAGGGCTGCCTAAAGGAAATGGTAGTTGTATC


 


ATCATAACAACTAGAATTGAAAAGGTTGCAAGAAAGATGGGTGTGAAAGAAGCAAGGATTCACAGGC


 


CTAAATTCCTTAACAAAGATTATAGCTGGCTTCTGTTTCGAAAAATAGCTTTTGCTGCAAGTGGTGGAG


 


AGTGCACTTCCACTGATCTTGAGGATGTTGGAAAAGAGATTGTAGAGAAGTGTAAAGGTCTTCCCTTG


 


GCCATCAAAGCTGTTGGAGGAATGATGCTTTGTAAAGCACCATATTACCGTGAATGGAGGCGAATTGC


 


AGATCATTTCAGAGATGAGTTGGAAGAAAATGATAACTCTGTCATGGCTTCACTACAATTGAGTTATGA


 


TGAGCTCCCTTCCTACTTGAAGTCCTGCTTGCTTAGCTTCTCTCTTTATCCTGAGGATTGTGTCATAACA


 


AAAGAGCAATTAGTTCATTGGTGGATTGGTGAGGGATTTGCCCCACAAAGGAGTAGTAGATCTTCAAC


 


TGATGCTGGAGAAGATTGTTTCTCAGGATTAACAAATCGATGTTTGTTAGAGGTAGTCGACAAGACTT


 


ATAATGGTACAATTTGCACTTGCAAAATGCATGATATGGTTCGTGATTTGGTTCTTAAGATAGCAAAAG


 


ATGATGCATTTTACAATGCAACGGGTACTAATTATCGCCATTTGGGAGTTGATAACAGCATGGATAAGA


 


AGCAACTTATTGCTAATCAGAAGTTGAGAGGATTGGTGTCCACAACAAAAACTTGTGAGGTGAACAA


 


GATTGAATCAGGTATTGCAAAAAGATTTAGTGAGTGCAAATACTTGAGAGTTTTGGATGTTTCCAAATC


 


AATATTTGAATTGCCTCTCAGTAGCTTGCTATATCGTGTTGGCACACTTCAACATCTGACTTATCTTGGTT


 


TAAGCAACACTCATCCTTTGGTTGAACTTCCAGATTCACTAGAAAATCTCACTAACCTTCAAATTTTGGA


 


TGTTAGTTACTGTCAGAATCTGAAATTCTTGCCTCAGTATCTCATTAAATTCAAGAAGCTCAAAGTCTTA


 


GATGTGAGCTTTTGTGGTTCCCTAGAAAACTTACCAAAAGGCTTGGGAAGGCTTTCAAACCTTGAAG


 


TTTTGCTGGGTTTTAGACCTGCAAGGTCAAATAATGGTTGCAGGATTGGAGAATTAAGAAACTTGACG


 


CGATTGAGGACGCTTGGATTACATCTAACACACGCTGATGAAGTTGAAGATAGCGAGTTCAACGCGAT


 


GATGAACCTTCAAGACTTGGAAAAGCTATCAATAAGTTTCTTTGATAGCCATGGCAGTGCAAGTGATCT


 


GACCTCTAAAATTGACAAACTCTGCCCTCCACAACAACTCCATGAACTATCCGTCATGTTCTATCCAGGA


 


AAGATAAGTCCACTTTGGCTCAACCCTTTAGCACTTCCCATGCTTAAATATCTGTCAATCTCTTCAGGAA


 


ATCTTGCAAAAATGCATCAGAACTTTTGGGGTGGTGACAACAACATTGTTTGGAAAGTTGAAGGCTTA


 


ATGTTGGAATCATTGTCTGATTTGGAACTGCAGTGGCCAAAGTTGCAACAGCTTATGCAAATCCTGCG


 


AGTTGTGAATGTTAGTTGGTGTCCGGAATTAGTTTCTTTCCCAATTGAAGATGTTGGATTCAGGGGTG


 


GAGTATGGATTAAGGAACAAATTAGGAACTGA


 


Organism Name: Corchorus olitorius


Type: PRT


Length: 854


Sequence name: Resistance gene analog 1 (RGA1) peptide


SEQ ID NO: 26



M A D A I V N V F L E K L L S T L A E E G R Y V T E F R D Q F E K L Q T E L Q L L Q C F L K D A D R



 


L K R K N H T I R K I L A D L R E L I Y E A E D I L A D C Q L Q S R D E N Q F S Q S W L A C F S P P


 


K L H F K Y Q S G K R L K E I T E K I T S I K Q N I S S F L G G P L L F Q P E V I S A Q D Q M P R W


 


S S Q V Y D H T Q V V G L E S D T Q K M K D W I F D A V H E G A Q E I L A I G V V G M G G L G


 


K T T I A Q K V F N E R D I E H H F D R R M W V S V S Q T F T E E Q I M R S M L R N L G D A S V


 


G D D R N E L L K K I N Q Y L L G K R Y L I V M D D V W S E D V L W W Q R I C E G L P K G N G S


 


C I I I T T R I E K V A R K M G V K E A R I H R P K F L N K D Y S W L L F R K I A F A A S G G E C T S


 


T D L E D V G K E I V E K C K G L P L A I K A V G G M M L C K A P Y Y R E W R R I A D H F R D E L


 


E E N D N S V M A S L Q L S Y D E L P S Y L K S C L L S F S L Y P E D C V I T K E Q L V H W W I G E


 


G F A P Q R S S R S S T D A G E D C F S G L T N R C L L E V V D K T Y N G T I C T C K M H D M V


 


R D L V L K I A K D D A F Y N A T G T N Y R H L G V D N S M D K K Q L I A N Q K L R G L V S T T K


 


T C E V N K I E S G I A K R F S E C K Y L R V L D V S K S I F E L P L S S L L Y R V G T L Q H L T Y L G


 


L S N T H P L V E L P D S L E N L T N L Q I L D V S Y C Q N L K F L P Q Y L I K F K K L K V L D V S F


 


C G S L E N L P K G L G R L S N L E V L L G F R P A R S N N G C R I G E L R N L T R L R T L G L H L


 


T H A D E V E D S E F N A M M N L Q D L E K L S I S F F D S H G S A S D L T S K I D K L C P P Q Q


 


L H E L S V M F Y P G K I S P L W L N P L A L P M L K Y L S I S S G N L A K M H Q N F W G G D N


 


N I V W K V E G L M L E S L S D L E L Q W P K L Q Q L M Q I L R V V N V S W C P E L V S F P I E


 


D V G F R G G V W I K E Q I R N


 


Organism Name: Corchorus olitorius


Type: DNA


Length: 3604


Sequence Name: Resistance gene analog 2 (RGA2); partial cDNA


SEQ ID NO: 27



AAAAGTAGACATGGCGGGTGATTATTCGGTGGAGCTTTTCTCTCTGCCACTCTCCAAGTTTTGTTCG



 


ACCGGTTGGCTTCTCGTGAGGTGGTGGACTTCATCAGGGGAAAGAAACTTGAGGTTTTGGTCAAGA


 


AACTGAAGCCAGTGTTGCTGTCCGTCAAAGCAGTGCTGGATGATGCTGAAGACAAGCAGATCACCAA


 


CCAGAATGTGAAAGAGTGGCTCTCCGAGCTCAAAGATGGTGTTTATGATGCAGAGGACCTCCTCGATG


 


AGATCGCTTATGAAGCTCTTAAAAGGAGGCTGGAAACCACTACTTCAGCCAAGGGGATATTGGAGTC


 


AAAGTTAGAGGAGATCCTTGAGAGGCTAGAACTTCTAGTCGACCAAACAGAACGCCTGGGGTTGAA


 


GGAGTGTAGAGGTGGTGAAACCTTGTCTCAAAGGTTGCCTCCAACTTCTGTGGTGGATGAGTCTTGT


 


GTTTATGGTAGAGTTGATGAAAAAGAAGCAATCATGAAGTTGCTACACCCTGAAAACCCGACTCAGAA


 


TCAGATTGATGTGATTCCCATAGTGGGTATGGGAGGGGTTGGTAAAACCACCCTTGCTCAATTGATCTA


 


CAATGACAACAGATTGGAGGAATGGTTTGACCTCAAAGCTTGGGTGTGTGTTTCAGATGAATTTGATG


 


CTTTCAGGGTTACCAAAACCATTCTTCAACAGATTGCTTCTGATTGGGATGATCGTCTCGACCTAAATC


 


AGCTTCAAGTTAAACTACAGGAGAAGCTGTTGGGGAAGAGGTTTCTATTTGTTTTAGATGATGTTTGG


 


AATGACAAATATATTGAGTGGAAACAGTTGACAAGTCCTTTCAGTGCTGGGGCAAAAGACAGCAAGA


 


TTGTTGTGACTACACGTAGTGATAATGTTGCAAACATCATGAGGACAGTTCCAGCTTATCAGCTTCCAA


 


TCTTATCTGATTCTGATTGTTGCTTGTTATTTGCAAAGCATGCGTTCGTCAATACAAGCCCAAGTGAGCA


 


GCCAGATTTGAAGCTAATTGGGGAAGCAATTGTCAAAAGGTGCAAAGGGCTACCTCTAGCAGTGAAA


 


GCAGTTGGAGGTTTTCTTCGTTGGAAACTAATGTTGATGAATGGAGAAATATGAATATGGAAGAGCA


 


AAGGTATGAGTACATCAAAGATTTAGAGTCAAGGTCATTTTTCCAAAAATTAAGCGGGGATGAATCCT


 


GCTTTGTCATGCATGACCTCATCAGTGACTTGGCTAAATCTGTGTCTGGAGAATTTTTTTGCAGATTGG


 


AAGGTGGCGATGGAGGTTCATGTGTTATAACTAAAAAGACCCGCCATTTGTCTAATATCCAAGAACCTT


 


ATGATGTGCGTAAGAAATTTGAGACCTTATGTGAAGCAAAAGGTTTGCGGACATTCCTAACTCTGAATC


 


TGAAGTCATCATTTCTATCTTCTTCTTTTGTTACTAACAGGCTAATGGATGATTTGATTGTAAAATCAAGT


 


CGCTTACGAGTTCTTTCTTTGACTGATTATAATAATATTAATATCAGGGAAATACAAGAAGGAATTGGAA


 


ATTTGAAGCATTTGCGATATTTGGACCTCTCTAATACTTTAATTCAAAGGTTGCCGAACCGTGTGTGTAC


 


TTTGTATAATTTACAAACATTAAAATTGTTTGGATGTGGCAAGCTTGTTGAGTTGCCAAAAGATATGGG


 


AAGATTGATCAATATGCATCATCTTGATATCAGGGATACAGGGTTAAATTGGATGCCATCAGGAATAGG


 


AAAATTGAAAGATCTTAAAGTTTTAACAAATTTTTTTGTTGGTAAGCATAAGGGTTCAAGCATTGGTGA


 


GTTGGGAAAGCTGAAGCATCTACAAGGAAGTGTTGCCATTTGGAATTTACAAAATGTTGTTTGTGCAA


 


AGGATGCTATGGATGCCAATTTGAAGGACAAGGTGAACCTTAAGGAGTTGAGATTGATATGGAGTAA


 


GGATGCTGATGTTGATGATGATTCAGAGCGAGATAGAGAAGTACTTGAACAACTGGAGCCTCACACA


 


GACTTGCAGCATCTTGACATCATGTTTTATAGAGGTACCAGATTTCCAGAGTGGATTGGGCATTCTTCT


 


TTCTCAAAAGTAGTATCTATGGAGTTAATTGATTGTAAAAGTTGTGAATTGTTGCCCCCACTACGCCAAC


 


TATCATCCCTGAAATCTCTCTCTATTAGTGGGTGTGTTAAGATAGTTAGACTGGGTGATGAGTTCTACGG


 


TAGTGGTGATGCATTGAGTAACCCATTTGGATGTCTTGAAGTTCTAAAATTTGAGGATATGTCAGAATG


 


GGAAGAATGGATTTGTTTGAAGGAAGAAGCTTTCTCTAATCTACGAGAATTAGTCATAAGAGATTGTC


 


CCAAGTTAAGCAAGTCTTTGCCCAAGTACCTCCCTTGTTTAAAGAAACTTAAGATTAGAAGATGTGGA


 


AAGCTCGAAGGCATACTTCCAGAGGCACCAAGCATTGAAGAAGTGCAGCTAGAGGGGTGTGATGCC


 


TTGCAAATGGAGGCATTGCCAAGTGGGCTTAGAGAATTGCAGATTGATGGTTTGCGAATCAATGCTTC


 


CATATTGAAGCAGATGTTGCAACCTTGCACTATTCTTGAACAATTGCAAATTTCTAAGTGTAATAGGCTG


 


AGATCCCTTCCTGAAGGTAGTAATTTGCCTATGAGACTGAAGAAATTGAGAATCGAAGAGAGTAATGT


 


GTTGAATGATTCTAAAATCCTCATGTATACATCCCTTGAATCCTTAAAAATAAGAAATAGCAGGTGGAAT


 


GGGGTGGAATCTTTCCCATTAGGATCATTCCCTTTGCTAAATCGTCTTGACATAACGGGATGTGAAGAG


 


TTGAAGTGGATTATTGGTGCATCAGAGGGAGAAGATGCTCCTCTCTCATGTCGTCTCAATTCTTTGGAG


 


ATCTATAATTGCCCTAATTTTGTATGTTTTGAGAAATTAGAGGGATTCTGTGCCCCCAATTTGACATCAC


 


TTGAGCTGGTGGGATGTTCAAATTTAAAGGCATTGCCTGAGCAAATGCACTCCCTCTTCCCATCCCTTG


 


AAGAATTGTGGATATCATTTTGTCCAAAAATAGAGGGATTTCCAAAAGAGGGTTTACCCTCCACATTAA


 


AAGCTCTTTTTATCCAAGGAGGATGTAAGAAACTAATAAAGGGGATGATGAGGAGAGATAGAGATAC


 


GGAATGGGGCTTGCAATCACTCCCTTCACTTGAAGAGTTCATAATCTCAGGTGGTGGAGAAGAGATA


 


GAAGGGATAGAGAGTTTTCCAGATGAACATCTGCTGCCCTCTTCTCTTACCTCTCTTTCTATCTCTTATT


 


TTCCAAATCTAAAGAGTTTGGAGTCTAAGGGCTTTCAACACCTCACCTCTCTCCGTCAATTGGGTATCT


 


ACTTTTGTCCGAGGCTCCAATCCATACCGGAAAAGAGGGTCATTTCCTCTCTTTCTTATTTGGAGATTG


 


CAAAATGTCCAAAGCTGAGAGAAAATCGTGAAAAGGAGAAAGGCAAACATTGGCCCAACATTTCCCA


 


CATCCCTGTCATCAACTTTGATGGGTTTGATCCAGTCATTATATAG


 


Organism Name: Corchorus olitorius


Type: PRT


Length: 1200


Sequence name: Resistance gene analog 2 (RGA2) peptide


SEQ ID NO: 28



K V D M A G E L F G G A F L S A T L Q V L F D R L A S R E V V D F I R G K K L E V L V K K L K P V L



 


L S V K A V L D D A E D K Q I T N Q N V K E W L S E L K D G V Y D A E D L L D E I A Y E A L K R R


 


L E T T T S A K G I L E S K L E E I L E R L E L L V D Q T E R L G L K E C R G G E T L S Q R L P P T S V


 


V D E S C V Y G R V D E K E A I M K L L H P E N P T Q N Q I D V I P I V G M G G V G K T T L A Q L


 


I Y N D N R L E E W F D L K A W V C V S D E F D A F R V T K T I L Q Q I A S D W D D R L D L N Q


 


L Q V K L Q E K L L G K R F L F V L D D V W N D K Y I E W K Q L T S P F S A G A K D S K I V V T T


 


R S D N V A N I M R T V P A Y Q L P I L S D S D C C L L F A K H A F V N T S P S E Q P D L K L I G E


 


A I V K R C K G L P L A V K A V G G F L R W K L D V D E W R N M N M E E Q R Y E Y I K D L E S R


 


S F F Q K L S G D E S C F V M H D L I S D L A K S V S G E F F C R L E G G D G G S C V I T K K T R H


 


L S N I Q E P Y D V R K K F E T L C E A K G L R T F L T L N L K S S F L S S S F V T N R L M D D L I V


 


K S S R L R V L S L T D Y N N I N I R E I Q E G I G N L K H L R Y L D L S N T L I Q R L P N R V C T L


 


Y N L Q T L K L F G C G K L V E L P K D M G R L I N M H H L D I R D T G L N W M P S G I G K L K


 


D L K V L T N F F V G K H K G S S I G E L G K L K H L Q G S V A I W N L Q N V V C A K D A M D A


 


N L K D K V N L K E L R L I W S K D A D V D D D S E R D R E V L E Q L E P H T D L Q H L D I M F Y


 


R G T R F P E W I G H S S F S K V V S M E L I D C K S C E L L P P L R Q L S S L K S L S I S G C V K I


 


V R L G D E F Y G S G D A L S N P F G C L E V L K F E D M S E W E E W I C L K E E A F S N L R E L


 


V I R D C P K L S K S L P K Y L P C L K K L K I R R C G K L E G I L P E A P S I E E V Q L E G C D A L Q


 


M E A L P S G L R E L Q I D G L R I N A S I L K Q M L Q P C T I L E Q L Q I S K C N R L R S L P E G S


 


N L P M R L K K L R I E E S N V L N D S K I L M Y T S L E S L K I R N S R W N G V E S F P L G S F P


 


L L N R L D I T G C E E L K W I I G A S E G E D A P L S C R L N S L E I Y N C P N F V C F E K L E G F


 


C A P N L T S L E L V G C S N L K A L P E Q M H S L F P S L E E L W I S F C P K I E G F P K E G L P S


 


T L K A L F I Q G G C K K L I K G M M R R D R D T E W G L Q S L P S L E E F I I S G G G E E I E G I


 


E S F P D E H L L P S S L T S L S I S Y F P N L K S L E S K G F Q H L T S L R Q L G I Y F C P R L Q S I


 


P E K R V I S S L S Y L E I A K C P K L R E N R E K E K G K H W P N I S H I P V I N F D G F D P V I I


 


Organism Name: Corchorus olitorius


Type: DNA


Length: 20


Sequence Name: Forward primer 1


SEQ ID NO: 29



TGGACGAGCTAACCCAGATT



 


Organism Name: Corchorus olitorius


Type: DNA


Length: 20


Sequence Name: Reverse primer 1


SEQ ID NO: 30



GGACAAGTGCCATTGAAAGG



 


Organism Name: Corchorus olitorius


Type: DNA


Length: 20


Sequence Name: Forward primer 5


SEQ ID NO: 31



CTGCCATAACAAAACCACCA



 


Organism Name: Corchorus olitorius


Type: DNA


Length: 20


Sequence Name: Reverse primer 5


SEQ ID NO: 32



ATTCGGTTGGAGTATTTGCG



 


Organism Name: Corchorus olitorius


Type: DNA


Length: 20


Sequence Name: Forward primer 9


SEQ ID NO: 33



TCAACATCATGGGAGTGCAT



Organism Name: Corchorus olitorius


 


Type: DNA


Length: 20


Sequence Name: Reverse primer 9


SEQ ID NO: 34



TTTGAGGAACAAAGAGGCGT



 


Organism Name: Corchorus olitorius


Type: DNA


Length: 20


Sequence Name: Forward primer 13


SEQ ID NO: 35



GTGGGCAAAGACAAGGGATA



 


Organism Name: Corchorus olitorius


Type: DNA


Length: 20


Sequence Name: Reverse primer 13


SEQ ID NO: 36



GCTTGGGGAACTACCATTGT



 


Organism Name: Corchorus olitorius


Type: DNA


Length: 20


Sequence Name: Forward primer 25


SEQ ID NO: 37



TGGGAAGCGTCTTAAGGAAA



 


Organism Name: Corchorus olitorius


Type: DNA


Length: 20


Sequence Name: Reverse primer 25


SEQ ID NO: 38



CTGTTAAGGGCTGCCTAAAG

## Claims

1. A nucleic acid molecule comprising a cDNA sequence having at least 95% sequence identity to a nucleic acid sequence selected from the group consisting of: SEQ ID NO: 1, 3, and 5, wherein said nucleic acid molecule encodes a Corchorus olitorius Toll and Interleukin-1 Receptor domain at their N-terminus Nucleotide-Binding Site-Leucine-Rich Repeat (ColTIR-NBS-LRR) resistance protein.

2. An expression vector, comprising the nucleic acid molecule of claim 1.

3. A transgenic plant, comprising the expression vector of claim 2.

4. A material obtained from the transgenic plant of claim 3, wherein said material comprises the expression vector.

5. A seed from the transgenic plant of claim 3, wherein said seed comprises the expression vector.

6. A method for making a transgenic plant, comprising the steps of: transfecting at least one plant cell with the expression vector of claim 2; and growing said at least one plant cell into the transgenic plant.

7. A method of improving disease resistance in a jute plant, comprising the step of incorporating into the jute plant the nucleic acid molecule of claim 1.

8. The nucleic acid molecule of claim 1, wherein said cDNA has at least 98% sequence identity to the nucleotide sequence selected from the group consisting of SEQ ID NO: 1, 3, and 5.

9. The nucleic acid molecule of claim 1, wherein said cDNA has at least 99% sequence identity to the nucleotide sequence selected from the group consisting of SEQ ID NO: 1, 3, and 5.

10. The nucleic acid molecule of claim 1, wherein said cDNA has 100% sequence identity to the nucleotide sequence selected from the group consisting of SEQ ID NO: 1, 3, and 5.

11. The nucleic acid molecule of claim 10, wherein said cDNA is SEQ ID NO: 1.

12. The nucleic acid molecule of claim 10, wherein said cDNA is SEQ ID NO: 3.

13. The nucleic acid molecule of claim 10, wherein said cDNA is SEQ ID NO: 5.