Test Type Report #Test SystemComments Results2-hr Approximate lethal concentration (ALC)DuPont21871Rat, whole-bodyNot GLP;NotGuidelineThe FEA-1100 male rat, whole-body inhalation 2-hr approximate lethal concentration(ALC) was greater than 21,000 ppm. There were no deaths during the exposurerecovery period.4-hr LC50DuPont26154 RV1Rat, nose-onlyGLP;GuidelineThe FEA-1100 male and female rat, nose-only inhalation 4-hr LC50 was greater than102,900 ppm. There were no deaths during the exposure or recovery period.Cardiac sensitizationDuPont25453DogGLP;StandardProtocolThe FEA-1100 cardiac sensitization no-observed-adverse-effect level (NOAEL) andlowest-observed-adverse-effect level (LOAEL) in epinephrine challenged dogs was12,500 and 25,000 ppm, respectivelyDermal irritationDuPont22199RabbitGLP;GuidelineFEA-1100 was not a dermal irritant when tested in rabbits. No clinical toxicity wasobserved in the study.Bacterial reverse mutation assay (Ames)DuPont22597BacteriaGLP;GuidelineFEA-1100 was not mutagenic in the Bacterial Reverse Mutation Assay (Ames) testusingSalmonellastrains TA98, TA100, TA1535, and TA1537 andE. colistrain WP2uvrA with and without S9 activation.In vitro chomosome aberrationDuPont22598HumanlymphocytesGLP;GuidelineFEA-1100 did not produce structural or numerical chromosome aberrations in aninvitromammalian chromosome aberration test using human peripheral bloodlymphocytes either in the presence or absence of S9-activated test systems.5-day inhalation range-finderDuPont25309RatNot GLP;NotGuidelineMale and female rats were exposed to 0, 1000, 10,000 or 50,000 ppm FEA-1100 vianose-only inhalation for 6-hours per day for 5 consecutive days. Reduced body weightgain, reduced food consumption and degeneration of the olfactory epithelium,neutrophilic infiltration and luminal cellular debris in the nasal cavity were observed atthe 10,000 and 50,000 ppm exposure levels. The no-observed-adverse-effect level(NOAEL) was 1000 ppm.28-day inhalationDuPont25850RatGLP;GuidelineMale and female rats were exposed to 0, 2500, 5000 or 10,000 ppm FEA-1100 via noseonly inhalation for 6 hours per day, 5 days per week for 4 weeks. Reduced body weightgain and reduced food consumption were observed at the 5000 ppm and higherexposure concentrations. The no-observed-adverse-effect level (NOAEL) was 2500ppm.90-day inhalationDuPont17453-785RatGLP;GuidelineMale and female rats were exposed to 0, 500, 1500 or 10,000 ppm FEA-1100 viawhole-body inhalation for 6 hours per day, 5 days per week for 13 weeks. Reducedbody weight gain and reduced food consumption were observed at the 10,000 ppmexposure level. There were no adverse effects in the clinical or anatomical pathologyparameters in animals exposed to FEA-1100; therefore, the no-observed-adverse-effectlevel (NOAEL) was 1500 ppm.Pre-natal developmentDuPont17453-731RatGLP;GuidelineFemale rats were exposed to 500, 1500 or 10,000 ppm for 6 hours per day duringgestation day (GD) 6-20. Reduced body weight gain, reduced food consumption andreduced fetal body weight were observed at the 10,000 ppm exposure level. No fetalmalformations were observed at any exposure concentration. The no-observed-adverseeffect level (NOAEL) for maternal and fetal effects was 1500 ppm.Completed Studies CAS RN 692-49-9 - submitted with the PMN90-day inhalation NA RatGLP;GuidelineMale and female rats were exposed to 0, 3000, 4000, 5000 or 7500 ppm FEA-1100 viawhole-body inhalation for 6 hours per day, 5 days per week for 13 weeks. Based onverbal results, the no-observed-adverse-effect level (NOAEL) is 5000 ppm and 7500ppm for males and females, respectively. Male rats had significantly reduced bodyweight in the 7500 ppm exposure group, but no histopathological effects were observedin any test group. The study was initiated in August 2010 and the expected report dateis August 2011.In vivo micronucleus NA RatGLP;GuidelineThe micronucleus study was conducted as part of the on-going 90-day inhalation inrats. Based on verbal results, the test substance was negative for adverse effets in the invivo micronucleus test. The study was initiated in August 2010 and the expected reportdata is August 2011.Ready biodegradability**A100560***NAGLP;GuidelineFEA-1100 is not readily biodegradable. No transformation product was generatedunder the conditions of the test.Partition coefficient**A100561***NAGLP;GuidelineThe study was conducted using the HPLC Method for determining a substance'spartition coefficient (n-octanol/water). The partition coefficient (log Pow) value of thetest sustance was calculated to be 2.3.Algae growth inhibition test** - - -In progressFish acute toxicity test** - - -In progressDaphnia acute immobilization test** - - -In progressExpected dates of final reports: 3Q2011On-going Studies CAS RN 692-49-9**Testing Facility: Environmental Risk Assessment CenterMitsubishi Chemical Medience Corporation1000 Kamoshida-cho, Aoba-ku, Yokohama, Kanagawa-ken 227-0033,JAPAN***Testing facility report number