# Patent

## Title
Increasing leaf longevity and disease resistance by altering salicylic acid catabolism

## Abstract

The present invention relates to a transgenic plant having an altered level of salicylic acid 3-hydroxylase (“S3H”) protein, compared to that of a non-transgenic plant, where the transgenic plant displays an altered leaf senescence phenotype, relative to a non-transgenic plant. The present invention relates to a mutant plant comprising an inactivated gene encoding S3H protein, where the mutant plant displays a premature or precocious leaf senescence phenotype, relative to a non-mutant plant. The present invention also relates to methods for promoting premature or precocious leaf senescence in a plant, delaying leaf senescence in a plant, and making a mutant plant having a decreased level of S3H protein compared to that of a non-mutant plant, where the mutant plant displays a premature or precocious leaf senescence phenotype relative to a non-mutant plant. The present invention also relates to inducing or promoting pathogen resistance in plants.

## Background
This application is a national stage application under 35 U.S.C. §371 of PCT Application No. PCT/US2014/049418, filed Aug. 1, 2014, which claims the benefit of U.S. Provisional Patent Application Ser. No. 61/861,858, filed Aug. 2, 2013, and U.S. Provisional Application Ser. No. 61/913,691, filed Dec. 9, 2013, each of which is hereby incorporated by reference in its entirety.


This invention was made with government support under grant number DE-FG02ER15341 awarded by the Department of Energy, and NSF-MCB-0445596 awarded by the National Science Foundation. The government has certain rights in this invention.


FIELD OF THE INVENTION
The present invention relates generally to control of plant lifecycle and disease resistance, as well as compositions and methods relating to the same.
BACKGROUND OF THE INVENTION
Salicylic acid (“SA”) (2-hydroxy benzoic acid), a phenolic compound, has been studied for its medicinal use in humans for more than 200 years (Vlot et al., “Salicylic Acid, a Multifaceted Hormone to Combat Disease,” Annu. Rev. Phytopathol. 47:177-206 (2009)) and its role as a plant hormone in disease resistance, leaf senescence, flowering and thermogenesis have also more recently been investigated (Vlot et al., “Salicylic Acid, a Multifaceted Hormone to Combat Disease,” Annu. Rev. Phytopathol. 47:177-206 (2009) and Raskin, “Role of Salicylic Acid in Plants,” Annu. Rev. Plant Physiol. Plant Mol. Biol. 43:439-463 (1992)). The roles of SA in plant defense and the hypersensitive response (a fast form of programmed cell death or PCD) have been intensively investigated (Vlot et al., “Salicylic Acid, a Multifaceted Hormone to Combat Disease,” Annu. Rev. Phytopathol. 47:177-206 (2009) and Raskin, “Role of Salicylic Acid in Plants,” Annu. Rev. Plant Physiol. Plant Mol. Biol. 43:439-463 (1992)). Leaf senescence is a slow form of programmed cell death that allows plants to mobilize nutrients released from senescing cells to seeds, storage organs or actively growing tissues (Zhang et al., “An ABA-Regulated and Golgi-Localized Protein Phosphatase Controls Water Loss During Leaf Senescence in Arabidopsis,” Plant J. 69(4):667-678 (2012) and Gan & Amasino, “Making Sense of Senescence: Molecular Genetic Regulation and Manipulation of Leaf Senescence,” Plant Physiol 113(2):313-319 (1997)). Although the role of SA in leaf senescence and its underlying molecular mechanism have been less studied, there is some evidence that both disease defense and leaf senescence seem to share some components in SA signaling and regulation (Love et al., “Timing Is Everything: Regulatory Overlap in Plant Cell Death,” Trends Plant Sci. 13(11):589-595 (2008) and Rivas-San Vicente & Plasencia, “Salicylic Acid Beyond Defence: Its Role in Plant Growth and Development,” J. Exp. Bot. 62(10):3321-3338 (2011)).
Much research has been carried out on SA biosynthesis. There are two SA biosynthetic pathways in plants: the phenylalanine ammonia lyase (PAL) pathway and the isochorismate (IC) pathway; both pathways use the primary metabolite chorismate (Dempsey et al., “Salicylic Acid Biosynthesis and Metabolism,” Arabidopsis Book 9:e0156 (2011)). The chorismate-derived L-phenylalanine can be converted into SA via either benzoate intermediates or coumaric acid through a series of enzymatic reactions involving PAL, benzoic acid 2-hydroxylase (BA2H) and other uncharacterized enzymes (Leon et al., “Benzoic Acid 2-Hydroxylase, a Soluble Oxygenase From Tobacco, Catalyzes Salicylic Acid Biosynthesis,” Proc. Nat'l Acad. Sci. U.S.A. 92(22):10413-10417 (1995)). Chorismate can also be converted to SA via isochorismate in a two-step process involving isochorismate synthase (ICS) and isochorismate pyruvate lyase (IPL). In Arabidopsis, two ICS enzymes, which convert chorismate to isochorimate, have been identified; the IC pathway contributes approximately 90% of the SA production induced by pathogens and UV light (Wildermuth et al., “Isochorismate Synthase Is Required to Synthesize Salicylic Acid for Plant Defence,” Nature 414(6863):562-565 (2001) and Garcion et al., “Characterization and Biological Function of the ISOCHORISMATE SYNTHASE2 Gene of Arabidopsis,” Plant Physiol. 147(3):1279-1287 (2008)).
In plants, SA may undergo biologically relevant chemical modifications such as glucosylation, methylation and amino acid (AA) conjugation (Dempsey et al., “Salicylic Acid Biosynthesis and Metabolism,” Arabidopsis Book 9:e0156 (2011)). SA has been shown to be converted to SA sugar conjugates SA O-β-glucoside (SAG) and salicyloyl glucose ester (SGE) by SA glucosyltransferases (SAGT) (Lim et al., “The Activity of Arabidopsis Glycosyltransferases Toward Salicylic Acid, 4-Hydroxybenzoic Acid, and Other Benzoates,” J. Biol. Chem. 277(1):586-592 (2002) and Dean & Delaney, “Metabolism of Salicylic Acid in Wild-Type, ugt74f1 and ugt74f2 Glucosyltransferase Mutants of Arabidopsis thaliana,” Physiol. Plant 132(4):417-425 (2008)).
The SA glycosides are actively transported from the cytosol to the vacuole as an inactive storage form that can later be converted back to SA (Dean et al., “The Formation, Vacuolar Localization, and Tonoplast Transport of Salicylic Acid Glucose Conjugates in Tobacco Cell Suspension Cultures,” Planta 221(2):287-296 (2005)). Methylation inactivates SA but increases SA's membrane permeability and volatility, thus allows more effective long distance transport of this defense signal (Park et al., “Methyl Salicylate Is a Critical Mobile Signal for Plant Systemic Acquired Resistance,” Science 318(5847):113-116 (2007)). AA conjugation of SA at trace levels was found in infected Arabidopsis plants (Zhang et al., “Dual Regulation Role of GH3.5 in Salicylic Acid and Auxin Signaling During Arabidopsis-Pseudomonas syringae Interaction,” Plant Physiol. 145(2):450-464 (2007)). Recently, high levels of 2,3- and 2,5-dihydroxybenzoic acid (2,3-DHBA and 2,5-DHBA, respectively) sugar conjugates were detected in infected or aged Arabidopsis leaves, and they appeared to be the major inactive form of SA (Bartsch et al., “Accumulation of Isochorismate-Derived 2,3-Dihydroxybenzoic 3-O-beta-D-Xyloside in Arabidopsis Resistance to Pathogens and Ageing of Leaves,” J. Biol. Chem. 285(33):25654-25665 (2010)). SA in transgenic Arabidopsis plants expressing a bacterial salicylate hydroxylase (encoded by NahG) was shown to be converted to catechol; the NahG transgenic plants have thus been useful in plant defense and senescence studies involving SA (Friedrich et al., “Characterization of Tobacco Plants Expressing a Bacterial Salicylate Hydroxylase Gene,” Plant Mol. Biol. 29(5):959-968 (1995) and Yamamoto et al., “Salicylate Hydroxylase, a Monooxygenase Requiring Flavin Adenine Dinucleotide: I. Purification and General Properties,” J. Biol. Chem. 240(8):3408-3413 (1965)). However, the enzyme(s), presumably SA hydroxylases, responsible for the formation of 2,3- and 2,5-DHBA have yet to be identified in plants.
The present invention is directed to overcoming these and other deficiencies in the art.
SUMMARY OF THE INVENTION
One aspect of the present invention relates to a transgenic plant having an altered level of salicylic acid 3-hydroxylase (“S3H”) protein, compared to that of a non-transgenic plant, where the transgenic plant displays an altered leaf senescence phenotype, relative to a non-transgenic plant.
Another aspect of the present invention relates to a mutant plant comprising an inactivated gene encoding S3H protein, where the mutant plant displays a premature or precocious leaf senescence phenotype, relative to a non-mutant plant.
Yet another aspect of the present invention relates to a method for promoting premature or precocious leaf senescence in a plant. This methods involves providing a transgenic plant or plant seed transformed with a nucleic acid construct effective in silencing expression of a S3H protein capable of causing leaf senescence in a plant; and growing the transgenic plant or the plant grown from the transgenic plant seed under conditions effective to promote premature or precocious leaf senescence in the transgenic plant or the plant grown from the transgenic plant seed.
Another aspect of the present invention relates to a method for delaying leaf senescence in a plant. This method involves transforming a plant cell with a nucleic acid molecule encoding a S3H capable of causing leaf senescence in a plant operably associated with a promoter to obtain a transformed plant cell, where expression of the nucleic acid molecule in the plant cell causes delayed leaf senescence; and regenerating a plant from the transformed plant cell under conditions effective to delay leaf senescence in the plant.
Another aspect of the present invention relates to a method of making a mutant plant having a decreased level of S3H protein compared to that of a non-mutant plant, where the mutant plant displays a premature or precocious leaf senescence phenotype relative to a non-mutant plant. This method involves providing at least one cell of a non-mutant plant containing a gene encoding a functional S3H protein and treating the at least one cell of a non-mutant plant under conditions effective to inactivate said gene, thereby yielding at least one mutant plant cell containing an inactivated S3H protein encoding gene. This method also involves propagating the at least one mutant plant cell into a mutant plant, where the mutant plant has a decreased level of S3H protein compared to that of the non-mutant plant and displays a premature or precocious leaf senescence phenotype relative to a non-mutant plant.
Another aspect of the present invention relates to a method of inducing or promoting pathogen resistance in plants. This method involves providing a transgenic plant or plant seed transformed with a nucleic acid construct effective in silencing expression of a salicylic acid 3-hydroxylase protein capable of causing leaf senescence in a plant; and growing the transgenic plant or the plant grown from the transgenic plant seed under conditions effective to induce or promote pathogen resistance in the transgenic plant or the plant grown from the transgenic plant seed.
The plant hormone SA plays critical roles in plant defense, stress responses and senescence. While the SA biosynthesis is well understood, the pathways by which SA is catabolized remains elusive.
Described herein is the characterization of a novel SA 3-hydroxylase (“S3H”) that converts SA to 2,3-DHBA, a precursor of SA's major storage form 2,3-DHBA sugar conjugates, and plays a pivotal role in SA catabolism and homeostasis and in regulation of leaf senescence. S3H is senescence associated and is inducible by SA, and is thus a key part of a negative feedback regulation system of SA levels during senescence. The enzyme converts SA (with a Km 58.29 μM) to both 2,3-dihydroxybenzoic acid (2,3-DHBA) and 2,5-DHBA in vitro but only 2,3-DHBA in vivo. The s3h knockout mutants fail to produce 2,3-DHBA sugar conjugates, accumulate very high levels of SA and its sugar conjugates, and exhibit a precocious senescence phenotype. Conversely, the gain-of-function lines contain high levels of 2,3-DHBA sugar conjugates, extremely low levels of SA and its sugar conjugates, and display a significantly extended leaf longevity. This research reveals an elegant SA catabolic mechanism by which plants regulate SA levels by converting it to 2,3-DHBA to prevent SA over-accumulation. The research also provides strong molecular genetic evidence for an important role of SA in regulating the onset and rate of leaf senescence. The control of leaf senescence is a significant advance and permits the control and enhancement of crop productivity and quality. For instance, delay of leaf senescence may have certain agricultural benefits, including, but not limited to, increase in grain yield and biomass, improvement in horticultural performance, and/or enhanced tolerance to drought stress. In addition, the Examples herein describe results indicating that S3H has a role in plant defense.



BRIEF DESCRIPTION OF THE DRAWINGS
FIGS. 1A-1E show experimental results demonstrating senescence-associated and SA-induced expression of S3H in WT and in a T-DNA insertion line. FIG. 1A shows results demonstrating S3H was expressed during leaf senescence. YL, young leaves, ML, mature leaves, ES, early senescence leaves; LS, late senescence leaves. AtNAP, a senescence maker gene, was used as a positive control. FIG. 1B shows experimental results demonstrating that S3H was induced by SA. The SA-inducible gene PR1 served as a positive control. 0, leaves without treatment. M, leaves treated with mock solution for 36 hrs. FIG. 1C is an illustration of T-DNA insertions in line SALK_059907. FIG. 1D shows the characterization of T-DNA insertion line of FIG. 1C by RNA gel blot analysis of S3H expression in the early senescence leaves of wild type (WT) and the s3h mutant plants (s3h). Two independently isolated RNA samples were used. FIG. 1E is a table showing the primers used in the studies described herein.
FIGS. 2A-2H show experimental results relating to phenotypic analysis of s3h knockout and S3H overexpression lines. FIG. 2A is an image of s3h null mutant (right) exhibited an accelerated leaf senescence phenotype compared to WT (left) at 35 DAG. FIG. 2B is a graph of experimental results relating to chlorophyll contents in the 5th to 10th leaves (counted from bottom to top with the 1st the oldest) of WT and s3h mutant shown in FIG. 2A (bars show SE, n=6). FIG. 2C is a graph of experimental results Fv/Fm ratios of leaves of WT and s3h mutant shown in in FIG. 2A (bars show SE, n=6). FIG. 2D shows experimental results demonstrating S3H overexpression line 1 (S3HOE1, right) displayed a remarkably delayed leaf senescence phenotype compared with that of WT at 45 DAG. FIG. 2E is a graph of experimental results relating to chlorophyll contents of the 5th to 10th leaves from WT and S3HOE1 plant in FIG. 2D. Bars are SE, n=6. (F) Fv/Fm ratios of leaves of WT and S3HOE1 shown in FIG. 2A. Bars show SE, n=6. FIG. 2G is a graph of experimental results showing altered onset and pace of leaf senescence in s3h and S3HOE1 plants compared with those of WT. The bottom section of each bar indicates days from leaf emergence to the onset of senescence (visible yellowing at the leaf tip). The top section of each bar indicates the time period (days) it takes for senescence to progress from the first visible yellowing at the leaf tip to the leaf petiole. The 6th rosette leaves were used for the assay. Bars show SE, n=6. FIG. 2H is a graph showing the survival curve of WT, s3h mutant and S3HOE1 transgenic plant. Bars show SE, n=16.
FIGS. 3A-3B show experimental results demonstrating phenotypic and transcriptional changes in s3h and S3HOE1 plants compared with those of WT. FIG. 3A includes images showing phenotypes of s3h mutant, WT, and S3HOE plants at different ages (DAG, days after germination). FIG. 3B includes images of RNA gel blot analyses of expression of senescence-associated genes (SAG12 and SAG13) and SA responsive genes (EDS1, PAD4 and PR1) in leaves of WT, s3h and S3HOE1 plants at 25, 30, 35 and 40 DAG, respectively. All rosette leaves from two plants at each time point were harvested for RNA extraction.
FIGS. 4A-4H show experimental results relating to conversion of SA to 2,3-DHBA and 2,5-DHBA by recombinant S3H enzyme in vitro. FIG. 4A is a schematic showing the biochemical reaction catalyzed by recombinant S3H in vitro. FIG. 4B is a graph showing HPLC profiles of 30-min reaction of the recombinant S3H protein and empty vector extracts (Ev) incubated with SA. The authentic 2,3-DHBA and 2,5-DHBA were used as standards. FIG. 4C is a graph showing that the UV spectra of the enzymatic product 2,3-DHBA is identical to that of the 2,3-DHBA standard. FIG. 4D is a graph showing that the UV spectra of the enzymatic product 2,5-DHBA is identical to that of the 2,5-DHBA standard. The S3H enzymatic products 2,3-DHBA and 2,5-DHBA also have the same tandem ESI-mass spectra MS (as shown in FIG. 4E) and MS2 (as shown in FIG. 4F), respectively. FIG. 4G is a graph of experimental results showing the kinetics of the recombinant S3H (SA as the substrate). The mean and SE are shown, n=4. The metabolite profiles of SA in young and senescing plants of WT, s3h and S3HOE lines were analyzed using LC-MS/MS. The levels of free SA, SA sugar conjugates, SA derivatives including 2,3-DHBA, 2,5-DHBA and their sugar conjugates are summarized in FIG. 4H.
FIG. 5 is a schematic illustration of proposed SA metabolic pathways enriched with the newly discovered S3H in Arabidopsis. The SA 3-hydroxylase (S3H) indicated in bold reported here is induced by accumulating SA (indicated by a black arrow) and it catalyzes the SA to form 2,3-DHBA that will be subsequently conjugated by sugars by unknown enzymes to produce the storage form 2,3-DHBA sugar conjugates. The SA may also be converted to 2,5-DHBA (by an unknown enzyme) that is then converted to its sugar conjugates. In addition, SA can be catalyzed to produce its storage form salicylic sugar conjugates by enzyme UGT74F1 and UGT74F2. The methytransferase BSMT1 is responsible for the production of functional form methylsalicylic acid from SA while methyl easterase 1, 2, 4, 7, 9 are responsible for the reverse reaction from methylsalicylic acid to SA. Anabolically, two routes including isochorismate (IC) pathway and phenylalanine ammonia-lyase (PAL) pathway have been implicated in SA synthesis. In Arabidopsis the ICS pathway plays the main role while the PAL pathway plays a minor role, either directly or indirectly in the production of SA. The ICS1 and ICS2 are responsible for converting chorismate to isochorismate. The enzyme from isochorimate to SA is yet to be discovered.
FIGS. 6A-6C are images of experimental results relating to accelerated leaf senescence in the s3h knockout mutant and its complementation test with S3H genomic DNA. FIG. 6A is an image of an s3h knockout mutant has normal bolting time and did not show any difference to WT at 25 days after germination (DAG). FIG. 6B is an image of an s3h mutant showing a significantly accelerated leaf senescence phenotype at 42 DAG (the shoots were removed for better observation). FIG. 6C is an image showing the accelerated leaf senescence phenotype in s3h is restored by the S3H genomic fragment. The pGL3228 represent the complementary construct containing the intact S3H genomic DNA including its promoter region.
FIG. 7 is an image of an RNA gel blot analysis of constitutive S3H overexpression lines (S3HOEs). The 1st through 6th rosette leaves were harvested from 35-DAG transgenic plants for RNA extraction and RNA gel blot analysis.
FIG. 8 is a phylogeny of S3H and its homologues in Arabidopsis genome. The deduced protein of S3H was used to search the protein sequence of its homologues and the phylogenetic tree was generated with DNAMAN software (version 7).
FIG. 9 is an amino acid alignment of deduced S3H protein (SEQ ID NO:3) and flavanone 3-hydroxylase enzyme (F3H) (SEQ ID NO:49). The alignment was done with DNAMAN software (version 7).
FIGS. 10A-10B show experimental results relating to effects of temperature (FIG. 10A) and pH value (FIG. 10B) on the S3H enzyme activities. The optimal temperature is around 40° C. and optimal pH value is 6.0. The data are the means and SE of three replicates.
FIG. 11 is graph showing experimental results of S3H Expression in senescent leaves of WT, NahG, coi1 and ein2 mutants. S3H gene expression in senescent leaves of SA deficient line NahG, JA mutant coi1 and enthylene mutant ein2. The ratios of expression in senescing leaves of mutant and WT are shown. The data analyzed here were extracted from microarray data reported in a previous publication (Buchanan-Wollaston et al., “Comparative Transcriptome Analysis Reveals Significant Differences in Gene Expression and Signalling Pathways Between Developmental and Dark/Starvation-Induced Senescence in Arabidopsis,” Plant J. 42(4):567-585 (2005), which is hereby incorporated by reference in its entirety).
FIGS. 12A-12C show the time-course expression pattern of S3H (FIG. 12A), PR1 (FIG. 12B), SAG12 (FIG. 12C) after H. arabidopsis inoculation. The gene expression pattern of S3H is similar to pathogen induced marker gene PR1 after pathogen inoculation. The senescence specific expression marker gene SAG12 did not change during the inoculation, indicating the senescence was not significantly induced 6 days post inoculation. The data was extracted from microarray data reported in a previous publication (Wang et al., “Timing of Plant Immune Responses by a Central Circadian Regulator,” Nature 470(7332):110-114 (2011), which is hereby incorporated by reference in its entirety).
FIG. 13 is a graph showing results of an experiment demonstrating Resistant Phenotype of s3h mutant and Susceptible Phenotype S3HOE line Infected with P. syringae pv tomato DC3000. WT (left bars), s3h (center bars) and S3HOE1 (right bars) plants were infected with P. syringae DC3000, and the number of colony-forming units (cfu) per cm2 leaf area was determined after 0, 1 and 3 days on agar plates. Leaf discs of 3 infected plants were pooled for this experiment. Error bars indicate SD of three biological repeats. ***P<0.001**P<0.01, *P<0.05.
FIG. 14 shows an amino acid sequence alignment of S3H protein, as well as an alignment of nucleotide sequences encoding S3H protein, from Arabidopsis thaliana, Populus trichocarpa, Zea mays, Sorghum bicolor, Oryza sativa (rice), Hordeum vulgare (common barley), Triticum aestivum (wheat), Nicotiana tabacum, petunia, Glycine max (Soybean), cotton, Malus domestica (Apple), Brassica napus, Solanum tuberosum (Potato), and Solanum lycopersicum (Tomato) (amino acid sequences of SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 17, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO: 23, SEQ ID NO: 25, SEQ ID NO: 27, SEQ ID NO: 29, and SEQ ID NO: 31, respectively; nucleotide sequences of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 18, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 28, and SEQ ID NO: 30, respectively).



DETAILED DESCRIPTION OF THE INVENTION
One aspect of the present invention relates to transgenic plant having an altered level of salicylic acid 3-hydroxylase (“53H”) protein, compared to that of a non-transgenic plant, where the transgenic plant displays an altered leaf senescence phenotype, relative to a non-transgenic plant.
Nucleic acid molecules encoding S3H, as well as corresponding S3H amino acid sequences, each of which may be used in accordance with the present invention include those from a plant is selected from the group consisting of rice, corn, soybean, canola, potato, wheat, mung bean, alfalfa, barley, rye, cotton, sunflower, peanut, sweet potato, bean, pea, chicory, lettuce, endive, cabbage, brussel sprout, beet, parsnip, turnip, cauliflower, broccoli, radish, spinach, onion, garlic, eggplant, pepper, celery, carrot, squash, pumpkin, zucchini, cucumber, apple, pear, melon, citrus, strawberry, grape, raspberry, pineapple, tobacco, tomato, sorghum, sugarcane, banana, Arabidopsis thaliana, Saintpaulia, petunia, pelargonium, poinsettia, chrysanthemum, carnation, crocus, marigold, daffodil, pine, Medicago truncatula, Sandersonia aurantiaca, Populus trichocarpa, and zinnia. 
In one embodiment, SH3 includes the amino acid sequence of NxYPxCPxPxLxxGxxxHxDxxxxTxLLQD (SEQ ID NO: 1), where x is any amino acid. SEQ ID NO:1 is a consensus sequence of those S3H amino acid sequences aligned in FIG. 14. In one embodiment, nucleic acid molecules encoding S3H, as well as corresponding S3H amino acid sequences, include those from Arabidopsis thaliana, Populus trichocarpa, Zea mays, Sorghum bicolor, Oryza sativa (rice), Hordeum vulgare (common barley), Triticum aestivum (wheat), Nicotiana tabacum, petunia, Glycine max (Soybean), cotton, Malus domestica (Apple), Brassica napus, Solanum tuberosum (Potato), or Solanum lycopersicum (Tomato). Such nucleic acid molecules and amino acid sequences include the following:







Arabidopsis thaliana S3H coding cDNA sequence 


(SEQ ID NO: 2):


ATGGCAACTTCTGCAATATCTAAGCTCTTAGTGTCTGATTTCGCCTCCTC


 


CGTTCACATCCCTTCAAACTATGTCCGACCAATCTCCGACCGTCCGAACT


 


TGTCCGAGGTCGAGAGTTCTGGCGATTCCATCCCTCTGATCGATCTCCGG


 


GACCTCCATGGACCTAATCGAGCCGTAATTGTCCAACAACTTGCTAGTGC


 


GTGTTCCACTTATGGTTTCTTTCAGATCAAGAATCATGGAGTACCAGATA


 


CAACCGTCAATAAAATGCAAACCGTTGCGAGAGAGTTCTTCCATCAACCC


 


GAGAGCGAAAGAGTCAAACACTACTCCGCAGATCCAACAAAGACAACGAG


 


ACTCTCCACCAGTTTCAACGTCGGCGCAGACAAAGTCTTGAACTGGAGAG


 


ACTTCCTTAGACTCCATTGCTTTCCCATAGAAGATTTCATCGAAGAATGG


 


CCATCTAGTCCCATCTCTTTCAGAGAAGTCACAGCTGAATATGCCACGAG


 


CGTAAGAGCTTTGGTTTTGAGACTTCTTGAGGCCATCTCAGAGAGTTTAG


 


GCCTTGAAAGCGACCATATAAGCAATATATTAGGCAAACACGCTCAACAC


 


ATGGCGTTTAACTACTATCCGCCGTGTCCAGAACCCGAGCTAACTTACGG


 


ACTTCCCGGACATAAAGACCCAACCGTTATCACTGTCCTTCTTCAAGACC


 


AAGTCTCTGGTTTGCAAGTCTTTAAGGATGATAAATGGGTCGCTGTTAGT


 


CCAATTCCCAACACTTTCATCGTCAATATCGGCGACCAAATGCAGGTCAT


 


AAGCAATGATAAATACAAGAGTGTGCTCCATAGAGCCGTAGTAAACACCG


 


AGAACGAGCGGTTATCGATTCCGACTTTCTATTTCCCTTCAACAGATGCA


 


GTGATTGGTCCAGCACACGAGCTGGTCAATGAACAAGACTCTCTTGCCAT


 


TTACAGAACCTACCCGTTTGTTGAGTATTGGGACAAGTTTTGGAACAGAT


 


CACTTGCTACTGCGAGCTGTCTAGACGCCTTCAAAGCTCCAACAACCTAA


 


Arabidopsis thaliana S3H protein sequence 


(SEQ ID NO: 3):


MATSAISKLLVSDFASSVHIPSNYVRPISDRPNLSEVESSGDSIPLIDLR


 


DLHGPNRAVIVQQLASACSTYGFFQIKNHGVPDTTVNKMQTVAREFFHQP


 


ESERVKHYSADPTKTTRLSTSFNVGADKVLNWRDFLRLHCFPIEDFIEEW


 


PSSPISFREVTAEYATSVRALVLRLLEAISESLGLESDHISNILGKHAQH


 


MAFNYYPPCPEPELTYGLPGHKDPTVITVLLQDQVSGLQVFKDDKWVAVS


 


PIPNTFIVNIGDQMQVISNDKYKSVLHRAVVNTENERLSIPTFYFPSTDA


 


VIGPAHELVNEQDSLAIYRTYPFVEYWDKFWNRSLATASCLDAFKAPTT


 


Populus trichocarpa S3H homologous coding cDNA


sequence (SEQ ID NO: 4):


ATGGCTCCCACCGCCAAGCTACTACTAGCCGACCTTGCATCTTCAGGTGT


 


AAAACAAATTCCTTCCAACTTCATCCGTCCCATCTCCGACCGTCCGAATC


 


TCTCCGATGTTCAGATTTCGGATGGCTCGATTCCTCTAATTGACCTTCGT


 


GGCCTTGATGGTCCCAACCACTCTACTATAATCGAACAAATTGGCCAAGC


 


ATGCCAAAGGGATGGGTTCTTTCAGGTGAAGAATCATGGGATACCAGAGG


 


AAATGATCAGTATCATACTAAACATAGCTAGACAGTTCTTCAAATTGCCT


 


GAAAGTGAAAGGTTAAAAAATTACTCTGACGATCCCACTAAGACAACCAG


 


GTTGTCTACTAGTTTCAATATTAAGACAGAACAAGTTTCAAGCTGGAGAG


 


ATTTCTTGAGACTTCATTGTTATCCTCTCGAAGATTACGTACATGAATGG


 


CCTAGCAATCCTCCATCATTCAGGAAAGATGTGGCTGAATATTGCACAAG


 


TGTTAGAGGTCTAGTGTTGAGACTGCTTGAGGCCATATCCGAGAGCTTGG


 


GTTTGGAAAGAGACTATATTGATAAGAAATTAGGCGGGCATGGACAACAT


 


ATGGCTATGAACTACTATCCACCCTGTCCACAGCCAGAACTCACATATGG


 


ATTGCCTGGACACACCGACCCTAATTTAATCACCATCCTGTTACAAGATC


 


ACGTGCCTGGATTGCAGGTTCTAAGAAATGGCAAGTGGATTGCTGTGAAT


 


CCGATTCCCAATACTTTCATCGTCAACATCGGTGATCAAATGCAGGTACT


 


TAGCAATGATCGTTACAAGAGTGTGCTTCACCGAGCAGTTGTGAACAGTG


 


ATAAAGACCGAATATCTATACCGACGTTCTACTGTCCTTCACCGGATGCT


 


GTAATCGGGCCTCCAAAGGAGCTAGTCGACGACGAGCATCCTGCCGTCTA


 


TAGAGATTTTACGTACGGTGAATACTATGAGAAGTTTTGGAACAAGGGAC


 


TTGTAAAAGAATGTTGCTTGGACTTGTTCAAGCCTTCTAATAATACAACC


 


TAG


 


Populus trichocarpa S3H homologous protein


sequence (SEQ ID NO: 5):


MAPTAKLLLADLASSGVKQIPSNFIRPISDRPNLSDVQISDGSIPLIDLR


 


GLDGPNHSTIIEQIGQACQRDGFFQVKNHGIPEEMISIILNIARQFFKLP


 


ESERLKNYSDDPTKTTRLSTSFNIKTEQVSSWRDFLRLHCYPLEDYVHEW


 


PSNPPSFRKDVAEYCTSVRGLVLRLLEAISESLGLERDYIDKKLGGHGQH


 


MAMNYYPPCPQPELTYGLPGHTDPNLITILLQDHVPGLQVLRNGKWIAVN


 


PIPNTFIVNIGDQMQVLSNDRYKSVLHRAVVNSDKDRISIPTFYCPSPDA


 


VIGPPKELVDDEHPAVYRDFTYGEYYEKFWNKGLVKECCLDLFKPSNNTT


 


Zea mays S3H homologous coding cDNA sequence


(SEQ ID NO: 6):


ATGGCCCCAGCCATTTCCAAGCCTCTCCTTACCGATCTCGTTGCACAGAT


 


CGGGAAGGTCCCATCGAGCCACATCAGGCCTGTCGGAGACCGCCCGGACC


 


TCGCCAATGTCGACAACGAGTCCGGCGCCGGGATCCCGCTCATCGACCTC


 


AAGAAGCTCAACGGCCCGGAGCGCCGTAAGGTGGTGGAGGCCATCGGCAA


 


GGCCTGCGAATCCGACGGCTTCTTCATGGTGACGAACCACGGCATCCCGG


 


CGGCGGTCGTGGAGGGCATGCTGCGCGTGGCGCGGGAGTTCTTCCACCTG


 


CCGGAGTCGGAGCGGCTCAAGTGCTACTCCGACGACCCCAACAAGGCGAT


 


CCGGCTGTCCACCAGCTTCAACGTGCGCACGGAGAAGGTCAGCAACTGGC


 


GCGACTTCCTGCGCCTGCATTGCTACCCCCTCCAGAGCTTCGTCGACCAG


 


TGGCCGTCAAACCCGCCGTCCTTCAGGCAAGTGGTGGGCACCTACGCGAC


 


GGAGGCCAGGGCGCTGGCGCTGAGGCTGCTGGAGGCCATATCGGAGAGCC


 


TGGGCCTGGAGCGGAGCCACATGGTGGCGGCCATGGGGAGGCACGCGCAG


 


CACATGGCGGTGAACTACTACCCGCCGTGCCCGCAGCCGGAGCTCACCTA


 


CGGGCTGCCGGGCCACAAGGACCCCAATGCCATCACGCTGCTGCTGCAGG


 


ACGGCGTCTCCGGCCTCCAGGTGCAGCGTGGCGGCCGCTGGGTGGCCGTC


 


AACCCCGTGCCCAACGCGCTGGTCATCAACATCGGAGACCAGATGCAGGC


 


ACTGAGCAACGACCGGTACAAGAGCGTGCTCCACCGCGTGATCGTCAACA


 


GCGAGAGCGAGCGGATCTCGGTGCCGACGTTCTACTGCCCGTCCCCGGAC


 


GCGGTGATCGCGCCGGCCGACGCGCTGGTGGACGACGGCCACCCTCTGGC


 


CTACCGCCCCTTCACTTACCAGGAGTACTACGACGCGTTCTGGAACATGG


 


GCCTCCAGTCGGCCAGCTGCCTCGACCGGTTTAGGCCCGGAGGATCGTTG


 


GAGTGA


 


Zea mays S3H homologous protein sequence


(SEQ ID NO: 7):


MAPAISKPLLTDLVAQIGKVPSSHIRPVGDRPDLANVDNESGAGIPLIDL


 


KKLNGPERRKVVEAIGKACESDGFFMVTNHGIPAAVVEGMLRVAREFFHL


 


PESERLKCYSDDPNKAIRLSTSFNVRTEKVSNWRDFLRLHCYPLQSFVDQ


 


WPSNPPSFRQVVGTYATEARALALRLLEAISESLGLERSHMVAAMGRHAQ


 


HMAVNYYPPCPQPELTYGLPGHKDPNAITLLLQDGVSGLQVQRGGRWVAV


 


NPVPNALVINIGDQMQALSNDRYKSVLHRVIVNSESERISVPTFYCPSPD


 


AVIAPADALVDDGHPLAYRPFTYQEYYDAFWNMGLQSASCLDRFRPGGSL


 


E


 


Sorghum bicolor S3H homologous coding cDNA


sequence (SEQ ID NO: 8):


ATGGCCCCAGCCATTTCCAAGCCTCTCCTCAGCGATCTCGTGGCACAGAT


 


CGGGAAAGTCCCATCGAGCCACATCAGGCCTGTGGGAGACCGCCCGGACC


 


TCGCCAATGTCGACAACGAGTCCGGCGCCGGGATCCCGCTCATCGACCTC


 


AAGATGCTCAACGGGCCGGAGCGCCGTAAGGTGGTGGAGGCCATCGGCAG


 


GGCCTGCGAATCCGACGGCTTCTTCATGGTGACGAACCACGGCATCCCGG


 


CGGCGGTGGTGGAGGGGATGCTGCGCGTGGCGAGGGAGTTCTTCCACCTG


 


CCGGAGTCGGAGCGGCTCAAGTGCTACTCCGACGACCCCAAGAAGGCGAT


 


CCGGCTGTCCACCAGCTTCAACGTGCGCACGGAGAAGGTGAACAACTGGC


 


GCGACTTCCTGCGCCTGCATTGCTACCCGCTCGAGAGCTTCGTCGACCAG


 


TGGCCGTCAAACCCGCCATCCTTCAGGCAAGTGGTGGGCACCTACGCGAC


 


GGAAGCGAGGGCGCTAGCGCTGAGGCTGCTGGAGGCCATATCGGAGAGCC


 


TGGGCCTGGAGCGGAGCCACATGGTGCGCGCCATGGGGAGGCACGCGCAG


 


CACATGGCGGTGAACTACTACCCGCCGTGCCCGCAGCCGGAGCTCACCTA


 


CGGGCTGCCGGGCCACAAGGACCCCAATGCCATCACGCTGCTGCTCCAGG


 


ACGGCGTCTCCGGCCTGCAGGTGCAGCGCGGCGGCCGATGGGTGGCCGTG


 


AACCCCGTGCCCGACGCGCTGGTCATCAACATCGGAGACCAGATGCAGGC


 


ACTGAGCAACGACCGATACAAGAGCGTGCTCCACCGCGTGATCGTCAACA


 


GCGAGAGCGAGCGGATCTCGGTGCCGACGTTTTACTGCCCGTCGCCGGAC


 


GGGGTGATCGCGCCGGCCGACGCGCTGGTGGACGACGCCCACCCTCTGGC


 


CTACCGCCCTTTCACTTACCAGGAGTACTACGACGAGTTCTGGAACATGG


 


GCCTCCAGTCGGCAAGCTGCCTCGACCGGTTTAGGCCCGGAGGATCCATA


 


GAGTGA


 


Sorghum bicolor S3H homologous Protein sequence


(SEQ ID NO: 9):


MAPAISKPLLSDLVAQIGKVPSSHIRPVGDRPDLANVDNESGAGIPLIDL


 


KMLNGPERRKVVEAIGRACESDGFFMVTNHGIPAAVVEGMLRVAREFFHL


 


PESERLKCYSDDPKKAIRLSTSFNVRTEKVNNWRDFLRLHCYPLESFVDQ


 


WPSNPPSFRQVVGTYATEARALALRLLEAISESLGLERSHMVRAMGRHAQ


 


HMAVNYYPPCPQPELTYGLPGHKDPNAITLLLQDGVSGLQVQRGGRWVAV


 


NPVPDALVINIGDQMQALSNDRYKSVLHRVIVNSESERISVPTFYCPSPD


 


GVIAPADALVDDAHPLAYRPFTYQEYYDEFWNMGLQSASCLDRFRPGGSI


 


Oryza sativa (rice) S3H homologous Coding cDNA


sequence (SEQ ID NO: 10):


ATGGCTCCAGCCATTGCCAAGCCTCTCCTGAGCGATCTGGTGGCACAATC


 


CGGGCAAGTCCCCTCGAGCCACATTCGTCCGGTTGGCGACCGCCCGGACC


 


TCGACAACGTCGACCACGAGTCCGGCGCCGGCATTCCGGTCATCGACCTG


 


AAACAGCTCGACGGCCCGGATCGCCGCAAGGTTGTCGAGGCCATCGGTTC


 


GGCGTGCGAAACCGACGGTTTTTTCATGGTGAAGAATCACGGGATCCCGG


 


AGGAGGTGGTGGAAGGGATGCTGCGCGTGGCGAGGGAGTTCTTCCACATG


 


CCGGAGTCGGAGCGGCTCAAGTGCTATTCCGACGACCCCAAGAAGGCGAT


 


CCGGCTGTCGACGAGCTTCAACGTGCGCACCGAGAAGGTGAGCAACTGGC


 


GCGACTTCCTGCGCTTGCATTGCTACCCTCTCGAGAGCTTCATCGACCAG


 


TGGCCCTCCAACCCACCCTCCTTCAGGCAAGTGGTCGGCACCTACTCGAG


 


GGAGGCGAGGGCGCTGGCGCTGCGGTTGCTGGAGGCGATATCTGAGAGCC


 


TCGGGCTGGAGAGGGGCCACATGGTGTCGGCCATGGGGCGGCAGGCGCAG


 


CACATGGCGGTGAACTACTATCCGCCATGCCCACAGCCGGAGCTCACCTA


 


CGGCCTGCCGGGGCACAAGGACCCCAATGCCATCACGCTGCTGCTCCAGG


 


ACGGCGTCTCCGGCCTGCAGGTCCAGCGCAACGGCCGCTGGGTGGCCGTC


 


AACCCCGTGCCCGACGCCCTGGTCATCAACATCGGAGATCAAATCCAGGC


 


GCTGAGCAACGACCGGTATAAGAGCGTGCTCCACCGGGTGATCGTGAACA


 


GCGAGAGCGAGAGGATCTCCGTGCCGACGTTCTACTGCCCGTCCCCGGAC


 


GCGGTGATCGCGCCGGCCGGCGCGCTGGTGGACGGCGCCCTGCACCCGCT


 


GGCGTACCGGCCCTTCAAGTACCAGGCCTACTACGACGAATTCTGGAACA


 


TGGGCCTCCAGTCCGCCAGCTGCTTAGACCGGTTCCGGCCTAACGATCAG


 


GCCGTCTGA


 


Oryza sativa (Rice) S3H homologous protein


sequence (SEQ ID NO: 11):


MAPAIAKPLLSDLVAQSGQVPSSHIRPVGDRPDLDNVDHESGAGIPVIDL


 


KQLDGPDRRKVVEAIGSACETDGFFMVKNHGIPEEVVEGMLRVAREFFHM


 


PESERLKCYSDDPKKAIRLSTSFNVRTEKVSNWRDFLRLHCYPLESFIDQ


 


WPSNPPSFRQVVGTYSREARALALRLLEAISESLGLERGHMVSAMGRQAQ


 


HMAVNYYPPCPQPELTYGLPGHKDPNAITLLLQDGVSGLQVQRNGRWVAV


 


NPVPDALVINIGDQIQALSNDRYKSVLHRVIVNSESERISVPTFYCPSPD


 


AVIAPAGALVDGALHPLAYRPFKYQAYYDEFWNMGLQSASCLDRFRPNDQ


 


AV


 


Hordeum vulgare (common barley) S3H homologous


coding cDNA sequence (SEQ ID NO: 12):


ATGGCTCCGGCGATCGCCGCCAAGCCTCTCCTCAGTGATCTGGTGGCACA


 


AACCCGGCGAGTTCCGTCGAGCCACATCAGAGCGGTCGGAGACCGTCCGG


 


ACCTCGCCAATGTCGACCACGAGTCCGGCGCGGGCATTCCGCTCATCGAC


 


CTGAAGCACCTCGACGGGCCAGGGCGTCGCAGGGTCGTCGAGGCCATCGG


 


CTCGGCGTGCGAGAACGACGGTTTTTTCATGGTGACGAACCACGGCATCC


 


CGGAGGCGGTCGTGGACGGGATGCTGCGCGTGGCGAGGGAGTTCTTCCAC


 


CTGCCGGAGTCTGAACGGCTCAAGTGCTACTCAGACGACCCCAAGAAGGC


 


GATCCGGCTGTCCACGAGCTTCAACGTGCGCACGGAGAAGGTGAGCAACT


 


GGCGCGATTTCCTCCGCCTGCATTGCTACCCTCTCGAGAGCTTCATCGAC


 


CAGTGGCCCTCAAACCCGCCGGCCTTCAGGGAAGCAGTCGGCACCTACTC


 


GACGGAGGCGAGAGCGCTGGCGCTCAGGCTGCTGGAGGCGATATCGGAGA


 


GCCTTGGGCTCGAGAGAGGCCACATGGTGAAGGCCATGGGGCGGCACGCG


 


CAGCACATGGCGGTGAACTACTACCCGCCGTGCCCGCAGCCGGAGCTGAC


 


GTACGGACTGCCGGGCCACAAGGACCCCAATGCCGTCACGCTGCTCCTCC


 


AGGACGGCGTGTCCGGGCTTCAGGTCCGGCGCGACGGCCGCTGGGTCGCC


 


GTCAACCCCGTGCCCGGCGCGTTGGTCATCAACATCGGCGATCAACTGCA


 


GGCTCTGAGCAACGACCGATACAAGAGCGTACTTCACCGGGTGATTGTGA


 


ACAGCGAGAGCGAGAGGATCTCGGTGCCGACGTTCTACTGCCCGTCCCCG


 


GACGCGGTGGTCGCGCCGGCGGAGGCGCTGGTGGACGGCGGCCACCGTCT


 


GGCCTATCGGCCCTTCACCTACCAGGAGTACTACGAGGAGTTCTGGAACA


 


TGGGCCTCGAGGCCGCCAGCTGCCTCGACCGCTTCCGACCGATCGCGTGA


 


Hordeum vulgare (common barley) S3H homologous


Protein sequence (SEQ ID NO: 13):


MAPAIAAKPLLSDLVAQTRRVPSSHIRAVGDRPDLANVDHESGAGIPLID


 


LKHLDGPGRRRVVEAIGSACENDGFFMVTNHGIPEAVVDGMLRVAREFFH


 


LPESERLKCYSDDPKKAIRLSTSFNVRTEKVSNWRDFLRLHCYPLESFID


 


QWPSNPPAFREAVGTYSTEARALALRLLEAISESLGLERGHMVKAMGRHA


 


QHMAVNYYPPCPQPELTYGLPGHKDPNAVTLLLQDGVSGLQVRRDGRWVA


 


VNPVPGALVINIGDQLQALSNDRYKSVLHRVIVNSESERISVPTFYCPSP


 


DAVVAPAEALVDGGHRLAYRPFTYQEYYEEFWNMGLEAASCLDRFRPIA


 


Triticum aestivum (wheat) S3H homologous Coding


cDNA sequence (SEQ ID NO: 14):


ATGGCGCCGGTGAGCAACGAGACGTTCCTCCCGACGGCGGCCTGGGGGGA


 


GGCGACGCTGCGCCCGTCCTTCGTGCGGGACGAGGACGAGCGGCCCAAGG


 


TGGCGCACGACCGCTTCAGCGATGCGGTGCCGGTGATCTCGCTCGATGGC


 


ATCGACGGCGCGCGCCGGGCCGAGATCCGGGACCGCGTGGCGGCGGCCTG


 


CGAGGGCTGGGGCATCTTCCAGGTGGTCGACCACGGCGTCGACGCCGACC


 


TCATCGCCGACATGACGCGCCTCTCTCGCGAGTTCTTCGCGCTGCCCGCC


 


GAGGACAAGCTCCGGTACGACATGTCCGGTGGCAAGAAGGGCGGCTTCAT


 


CGTCTCCAGCCACCTGCAGGGTGAGGCGGTGCAGGACTGGAGGGAGATTG


 


TGACCTACTTCTCGTACCCGGTGAAAGCACGGGACTACGGGCGGTGGCCG


 


GAGAAGCCGGCGGGGTGGCGCGCGGTAGTGGAGCGGTACAGCGAGCGGCT


 


GATGGAGCTGTCGTGCAAGCTGCTGGGCGTGCTCTCGGAGGCGATGGGCC


 


TGGAGACGGAGTCCCTGGCCAAGGCGTGCGTGGACATGGACCAGAAGGTG


 


GTGGTCAACTTCTACCCGCGGTGTCCCCAGCCCGAGCTCACCCTGGGCGT


 


CAAGCGCCACACCGACCCCGGCACCATCACCCTCCTCCTCCAGGACCTAG


 


TCGGCGGCCTGCAGGCCACCCGCGACGGCGGCAAGACCTGGATCACCGTC


 


CAGCCCATCTCCGGCGCCTTCGTCGTCAACCTCGGCGACCACGGCCACTT


 


CATGAGCAACGGCAGGTTCAAGAACGCGGACCACCAGGCGGTGGTGAACG


 


GGCAGAGCAGCCGGCTGTCGATCGCGACGTTCCAGAACCCGGCGCCGGAC


 


GCGAGGGTGTGGCCGCTGGCGGTGAGGGAGGGGGAGGAGCCCATACTGGA


 


GGAGCCCATCACCTTCTCCGAGATGTACCGCCGCAAGATGGAGCGCGACC


 


TCGACCTCGCCAAGCGCAAGAAGCAGGCCAAGGACCAGCTGATGCAGCAG


 


CAGCTCCAGCTCCAGCAGCAGCAGCAGGCGGTCGCCGCCGCGCCCATGCC


 


CACCGCCACCAAGTCTCTCAACGAAATTCTTGCCTAG


 


Triticum aestivum S3H homologous protein sequence


(SEQ ID NO: 15):


MAPVSNETFLPTAAWGEATLRPSFVRDEDERPKVAHDRFSDAVPVISLDG


 


IDGARRAEIRDRVAAACEGWGIFQVVDHGVDADLIADMTRLSREFFALPA


 


EDKLRYDMSGGKKGGFIVSSHLQGEAVQDWREIVTYFSYPVKARDYGRWP


 


EKPAGWRAVVERYSERLMELSCKLLGVLSEAMGLETESLAKACVDMDQKV


 


VVNFYPRCPQPELTLGVKRHTDPGTITLLLQDLVGGLQATRDGGKTWITV


 


QPISGAFVVNLGDHGHFMSNGRFKNADHQAVVNGQSSRLSIATFQNPAPD


 


ARVWPLAVREGEEPILEEPITFSEMYRRKMERDLDLAKRKKQAKDQLMQQ


 


QLQLQQQQQAVAAAPMPTATKSLNEILA


 


Nicotiana tabacum S3H homologous coding cDNA


sequence (SEQ ID NO: 16):


ATGGCACCTTCGACATTGACAGCTCTAGCAGAGGAAAAGACACTTCAAAC


 


AAGTTTCATAAGGGATGAAGATGAGCGTCCAAAAGTGGCTTATAATCAAT


 


TCAGTGACGAGATTCCGATCATATCGTTGAAGGGTATTGATGATGAGAGT


 


GGAATTAATGGAAAAAGAGGTGAAATATGTGAAAAGATTGTTAAGGCATG


 


TGAAGATTGGGGCATTTTCCAGGTAGTTGATCATGGTGTTGATGCCCAAC


 


TTATCTCACAAATGACAACCCTTGCTAAACAATTCTTCGCTTTGCCTCCT


 


GAGGAAAAACTACGCTTTGATATGTCTGGTGGCAAGAAAGGTGGCTTCAT


 


TGTCTCTAGCCATCTACAGGGTGAAGTGGTCCAAGATTGGCGTGAAATAG


 


TGACCTATTTCTCATATCCAATTCGGGCTAGAGACTACTCTAGATGGCCA


 


GACAAACCAGATGGATGGATAGGTGTGACTCAGAAGTACAGTGAAAAGTT


 


AATGGAGTTGGCTTGCAAATTATTGGAAGTACTATCAGAGGCAATGGGCT


 


TAGAGAAGGAGGCCTTAACCAAGGCATGTGTGGATATGGACCAAAAAGTG


 


GTTGTCAATTTTTACCCAAAGTGTCCACAGCCCGACCTTACCCTTGGACT


 


GAAACGACACACTGATCCAGGAACCATTACCCTCTTGTTACAAGACCAAG


 


TTGGTGGGCTTCAAGCCACTAAAGATAATGGCAAAACTTGGATTACTGTT


 


CAGCCCGTTGAAGGCGCTTTTGTTGTCAATCTTGGTGACCATGGTCACTT


 


TTTGAGCAATGGAAGGTTTAAGAATGCTGATCATCAAGCAGTGGTGAACT


 


CGAATAGTAGCAGATTATCGATAGCTACGTTTCAGAATCCAGCACCAGAA


 


GCTATAGTGTACCCATTGAAAATTAGGGAAGGAGAGAAGGCAGTAATGGA


 


CGAGCCCATAACATTTGCAGAGATGTACAGGAGGAAAATGAGCAAGGACC


 


TTGAGCTTGCTAGGCTCAAGAAACTGGCCAAGGAACACCAAATACAAGCT


 


GAAAAAGCTGCTGAGAAGGCCAAGTTGAAAACCAAGCCCATTGAAGAAAT


 


TCTTGCTTAA


 


Nicotiana tabacum S3H homologous protein sequence


(SEQ ID NO: 17):


MAPSTLTALAEEKTLQTSFIRDEDERPKVAYNQFSDEIPIISLKGIDDES


 


GINGKRGEICEKIVKACEDWGIFQVVDHGVDAQLISQMTTLAKQFFALPP


 


EEKLRFDMSGGKKGGFIVSSHLQGEVVQDWREIVTYFSYPIRARDYSRWP


 


DKPDGWIGVTQKYSEKLMELACKLLEVLSEAMGLEKEALTKACVDMDQKV


 


VVNFYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATKDNGKTWITV


 


QPVEGAFVVNLGDHGHFLSNGRFKNADHQAVVNSNSSRLSIATFQNPAPE


 


AIVYPLKIREGEKAVMDEPITFAEMYRRKMSKDLELARLKKLAKEHQIQA


 


EKAAEKAKLKTKPIEEILA


 


Petunia S3H homologous coding cDNA sequence


(SEQ ID NO: 18):


ATGAAAACAGCTCAAGGTGTCAGTGCAACCCTAACAATGGAAGTGGCAAG


 


AGTACAAGCAATAGCATCGTTAAGCAAGTGCATGGACACAATTCCATCAG


 


AGTACATTAGGTCCGAGAATGAGCAACCAGCAGCCACAACCCTGCATGGG


 


GTAGTTCTTCAAGTGCCAGTGATTGACCTACGTGACCCTGATGAGAACAA


 


GATGGTGAAGCTCATAGCTGATGCTAGCAAAGAGTGGGGGATATTCCAAC


 


TGATCAACCATGGCATTCCTGATGAGGCTATCGCGGATTTACAGAAAGTA


 


GGGAAAGAGTTCTTTGAACATGTTCCACAGGAGGAGAAAGAGCTGATTGC


 


CAAGACTCCAGGATCAAACGACATTGAAGGCTATGGAACTTCTCTGCAGA


 


AGGAAGTGGAAGGCAAGAAAGGTTGGGTGGATCATTTGTTCCATAAGATT


 


TGGCCTCCTTCTGCCGTCAACTATCGTTATTGGCCTAAAAACCCTCCTTC


 


ATACAGGGAAGCAAACGAAGAATATGGAAAGAGGATGCGAGAAGTTGTAG


 


ACAGAATTTTTAAGAGCTTGTCTCTTGGGCTTGGGCTTGAAGGCCATGAA


 


ATGATAGAGGCAGCTGGTGGTGATGAGATAGTTTACTTGTTGAAGATCAA


 


CTATTACCCACCATGCCCAAGGCCCGATTTGGCTCTTGGTGTTGTGGCCC


 


ATACGGACATGTCATATATCACCATTCTTGTCCCAAATGAAGTCCAAGGC


 


CTCCAAGTGTTCAAGGATGGCCATTGGTATGATGTCAAGTACATACCAAA


 


TGCCTTAATTGTCCATATTGGTGACCAAGTTGAGATTCTTAGCAATGGCA


 


AATACAAGAGTGTATACCATAGGACAACGGTGAACAAGGACAAGACAAGA


 


ATGTCATGGCCGGTTTTCTTGGAGCCCCCGTCAGAGCATGAAGTTGGGCC


 


AATTCCTAAGCTGCTTAGTGAGGCCAACCCACCCAAATTCAAGACCAAGA


 


AGTACAAGGATTACGTCTATTGTAAGCTTAACAAGCTTCCTCAGTGA


 


Petunia S3H homologous protein sequence


(SEQ ID NO: 19):


MKTAQGVSATLTMEVARVQAIASLSKCMDTIPSEYIRSENEQPAATTLHG


 


VVLQVPVIDLRDPDENKMVKLIADASKEWGIFQLINHGIPDEAIADLQKV


 


GKEFFEHVPQEEKELIAKTPGSNDIEGYGTSLQKEVEGKKGWVDHLFHKI


 


WPPSAVNYRYWPKNPPSYREANEEYGKRMREVVDRIFKSLSLGLGLEGHE


 


MIEAAGGDEIVYLLKINYYPPCPRPDLALGVVAHTDMSYITILVPNEVQG


 


LQVFKDGHWYDVKYIPNALIVHIGDQVEILSNGKYKSVYHRTTVNKDKTR


 


MSWPVFLEPPSEHEVGPIPKLLSEANPPKFKTKKYKDYVYCKLNKLPQ.


 


Glycine max (Soybean) S3H homologous coding cDNA


sequence (SEQ ID NO: 20)


ATGGCCACCACAAAGCCATTGTTAACCGACTTAGCCTCCACCGTTGATCG


 


TGTTCCCTCTAACTTCATCAGGCCCATTGGTGACCGTCCAAACCTTCAGC


 


AACTTCACTCCTCCATTGCTTCTATTCCCATCATCGACCTTCAAGGCCTT


 


GGTGGCTCCAATCATTCCCAAATCATCCAAAACATTGCACATGCTTGCCA


 


AAATTATGGCTTCTTTCAAATTGTGAACCATGGGATTCCGGAGGAGGTGG


 


TGAGCAAGATGGTGAATGTGTCAAAAGAGTTCTTTGGTTTGCCGGAGAGT


 


GAGAGGCTGAAGAATTACTCTGATGACCCAACCAAGACCACAAGACTCTC


 


CACCAGTTTCAATGTCAAGACTGAGAAAGTTTCCAACTGGAGAGACTTCT


 


TGAGACTTCACTGCCACCCCCTTGAGGATTACATTCAAGAATGGCCTGGC


 


AACCCTCCATCTTTCAGGGAAGATGTGGCGGAGTATAGTAGAAAGATGAG


 


GGGTTTATCACTGAAGTTGCTTGAGGCAATCTCAGAGAGTTTGGGGTTGG


 


AAAAGGATTATATAGACAAAGCATTGGGGAAACATGGGCAGCACATGGCC


 


ATAAACTACTACCCTCCATGTCCTGAGCCAGAGTTAACATATGGTTTGCC


 


AGCTCATGCTGACCCAAATGCAATTACTATTCTGCTCCAAAATCAAGTCC


 


CTGGCTTGCAAGTCCTCCATGATGGCAAGTGGCTAACCGTCAATCCTGTT


 


CCTAACACCTTCATTGTCAATATTGCTGACCAAATTCAGGTGATAAGCAA


 


CGATAGGTACAAGAGTGTGCTGCATCGAGCATTGGTGAATTGTGAGAAGG


 


AGAGAATGTCCATTCCAACATTCTATTGCCCTTCACCTGATGCATTGATA


 


AAACCAGCACCACAACTCGTAGACAAGGAACATCCTGCGCAGTACACAAA


 


CTTCACATACAGAGAATACTACGACAAGTTCTGGATCAGAGGACTTTCAA


 


AAGAAACATGCGTGGACATGTTCAAGGCTCAAGATTAA


 


Glycine max (Soybean) S3H homologous protein


sequence (SEQ ID NO: 21):


MATTKPLLTDLASTVDRVPSNFIRPIGDRPNLQQLHSSIASIPIIDLQGL


 


GGSNHSQIIQNIAHACQNYGFFQIVNHGIPEEVVSKMVNVSKEFFGLPES


 


ERLKNYSDDPTKTTRLSTSFNVKTEKVSNWRDFLRLHCHPLEDYIQEWPG


 


NPPSFREDVAEYSRKMRGLSLKLLEAISESLGLEKDYIDKALGKHGQHMA


 


INYYPPCPEPELTYGLPAHADPNAITILLQNQVPGLQVLHDGKWLTVNPV


 


PNTFIVNIADQIQVISNDRYKSVLHRALVNCEKERMSIPTFYCPSPDALI


 


KPAPQLVDKEHPAQYTNFTYREYYDKFWIRGLSKETCVDMFKAQD


 


Cotton S3H coding cDNA homologous sequence


(SEQ ID NO: 22):


ATGGCTCCTTCAACTCTGACGGCTCTTGCGGAAGAGAAAACCTTGCAGGC


 


AAGCTTCGTTCGTGATGAAGATGAGCGTCCTAAGGTTGCTTACAACCAAT


 


TCAGTAATGATATCCCTGTCATCTCTCTTGCTGGTATCGATGATGTTGAT


 


GGCAAGAGGGGTGAGATATGCAAGAAGATTGTTGAGGCTTGTGAGGATTG


 


GGGTGTCTTCCAGGTTGTGGATCATGGTGTTGATACTAAACTCGTGTCCG


 


AAATGACCCGTTTTGCCAGAGAGTTTTTTGCTTTGCCTGCTGAAGAGAAG


 


CTTCGGTTCGATATGTCTGGTGGCAAGAAAGGTGGTTTCATCGTCTCCAG


 


CCACCTTCAGGGAGAAGCAGTGCAAGATTGGCGGGAGATTGTGACATACT


 


TTTCATACCCATTGAAGAGCAGGGACTATTCAAGGTGGCCTGATAAGCCA


 


GAGGGTTGGATTGAAGTTACAAAGGAGTACAGCGAGAAGTTGATGGGCCT


 


AGCTTGCAAGCTTCTTGAGGTGTTGTCAGAGGCCATGGGGTTAGAGAAGG


 


AGGCTTTGACTAAGGCATGTGTGGACATGGATCAGAAAGTGGTGGTTAAC


 


TTCTATCCTAAATGCCCTCAACCTGACCTCACTTTAGGACTCAAGCGCCA


 


CACTGACCCAGGCACCATCACACTCTTGCTTCAAGACCAAGTTGGTGGGC


 


TTCAGGCCACCCGGGACAATGGCAAGACGTGGATCACTGTCCAACCTGTG


 


GAAGGAGCCTTTGTGGTCAACCTTGGAGACCATGGCCATTATCTGAGCAA


 


TGGGAGGTTCAAGAATGCTGATCACCAAGCAGTGGTGAACTCAAACTGCA


 


GCAGATTGTCAATAGCCACATTCCAAAATCCAGCACCCGATGCCACAGTG


 


TATCCCTTGAAGATCAGAGAGGGAGAGAAACCAATCCTTGAGGAGCCCAT


 


CACATTTGCTGAGATGTATAGGAGGAAGATGAGCAAGGATCTTGAGCTTG


 


CCAGGCTGAAGAAGCTGGCCAAAGAGCAGCAACAGTTGAAGGAGAAAGAG


 


GCTGAGAATGAGAAGCCCAAGCTTGAAGCCAAGCCATTGGAGGAAATCCT


 


TGCCTAA


 


Cotton S3H protein homologous sequence


(SEQ ID NO: 23):


MAPSTLTALAEEKTLQASFVRDEDERPKVAYNQFSNDIPVISLAGIDDVD


 


GKRGEICKKIVEACEDWGVFQVVDHGVDTKLVSEMTRFAREFFALPAEEK


 


LRFDMSGGKKGGFIVSSHLQGEAVQDWREIVTYFSYPLKSRDYSRWPDKP


 


EGWIEVTKEYSEKLMGLACKLLEVLSEAMGLEKEALTKACVDMDQKVVVN


 


FYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATRDNGKTWITVQPV


 


EGAFVVNLGDHGHYLSNGRFKNADHQAVVNSNCSRLSIATFQNPAPDATV


 


YPLKIREGEKPILEEPITFAEMYRRKMSKDLELARLKKLAKEQQQLKEKE


 


AENEKPKLEAKPLEEILA


 


Malus domestica (Apple) S3H homologous coding cDNA


sequence (SEQ ID NO: 24):


ATGGCTCCTCCTGCTACTACGCTGACATCCATTGCGCATGAGAAAACCCT


 


ACAACAAAAATTCGTCCGAGACGAAGACGAGCGTCCAAAGGTTGCCTACA


 


ACGAATTCAGCAACGAAATTCCGATCATCTCGCTTGCCGGGATCGATGAG


 


GTTGAAGGCCGCCGGGCCGAGATTTGCAAGAAGATTGTGGAAGCTTGTGA


 


GGACTGGGGTATTTTCCAGATTGTTGATCATGGAGTTGATGCCGAGCTCA


 


TATCGGAAATGACCGGTCTCGCCAAAGAGTTCTTTGATTTGCCATCGGAG


 


GAGAAGCTCCGCTTCGACATGTCCGGTGGCAAAAAGGGTGGATTCATCGT


 


GTCCAGTCATTTACAGGGAGAAGCTGTGCAAGATTGGCGTGAAATTGTGA


 


CCTACTTTTTATACCCGATTCGCCACCGGGACTACTCGAGGTGGCCGGAC


 


AAGCCAGAGGCATGGAGGGAGGTGACGAAGAAGTACAGCGACGAGCTGAT


 


GGGGCTGGCATGCAAGCTCTTGGGGGTTTTATCAGAAGCCATGGGGTTGG


 


ATACAGAGGCATTGACAAAGGCATGTGTGGACATGGACCAAAAAGTGGTG


 


GTGAATTTCTATCCGAAGTGCCCTCAGCCCGACCTAACTCTTGGCCTCAA


 


GCGCCACACGGACCCGGGCACAATTACCCTTTTGCTTCAGGACCAAGTTG


 


GTGGCCTTCAGGCTACTAGGGATGATGGGAAGACATGGATCACCGTTCAA


 


CCAGTGGAAGGAGCTTTTGTGGTCAATCTCGGAGATCATGGTCATTTTCT


 


GAGCAATGGGAGGTTCAAGAATGCTGATCACCAAGCAGTGGTGAACTCAA


 


ACAGCAGCAGGCTGTCCATAGCCACATTCCAGAACCCAGCTCAAGATGCA


 


ATAGTGTATCCACTCAGTGTGAGGGAGGGAGAGAAGCCGATTCTCGAGGC


 


GCCGATCACCTACACCGAGATGTACAAGAAGAAGATGAGCAAAGATCTTG


 


AGCTTGCCAGGCTGAAAAAGCTGGCCAAGGAACAGCAACTGCAGGACTTG


 


GAGAAAGCCAAAGTGGAGACAAAGCCAGCGGACGACATATTTGCTTAG


 


Malus domestica (Apple) S3H homologous protein


sequence (SEQ ID NO: 25):


MAPPATTLTSIAHEKTLQQKFVRDEDERPKVAYNEFSNEIPIISLAGIDE


 


VEGRRAEICKKIVEACEDWGIFQIVDHGVDAELISEMTGLAKEFFDLPSE


 


EKLRFDMSGGKKGGFIVSSHLQGEAVQDWREIVTYFLYPIRHRDYSRWPD


 


KPEAWREVTKKYSDELMGLACKLLGVLSEAMGLDTEALTKACVDMDQKVV


 


VNFYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATRDDGKTWITVQ


 


PVEGAFVVNLGDHGHFLSNGRFKNADHQAVVNSNSSRLSIATFQNPAQDA


 


IVYPLSVREGEKPILEAPITYTEMYKKKMSKDLELARLKKLAKEQQLQDL


 


EKAKVETKPADDIFA


 


Brassica napus S3H Coding cDNA homologous sequence


(SEQ ID NO: 26):


ATGGCTCCAGGAACTCTAAATGAGCTTGCCGGAGAGACTAAGCTCAACTC


 


CAAGTTTGTCCGGGACGAGGACGAACGTCCCAAGGTTGCCTACAATGAGT


 


TTAGCACGGAGATCCCCGTGATCTCTCTCGCCGGAATCGACGATGTTGGT


 


GAGAAAAGAGGAGAGATCTGTCGACAGATCGTTGAGGCTTGTGAGAACTG


 


GGGTGTTTTTCAGGTGGTCGATCATGGAGTGGATACTAGTTTGGTGGCCG


 


ATATGACTCGTCTCGCTCGAGACTTCTTCGCGTTACCTCCTGAGGAGAAA


 


CTCAAGTTCGACATGTCTGGTGGTAAAAAGGGAGGATTCATTGTCTCTAG


 


TCATCTTCAGGGAGAGTCTGTTCAAGATTGGAGAGAGATCGTGACGTATT


 


TCTCGTACCCGGTGAGAAACAGAGACTACTCACGGTGGCCGACTAAGCCG


 


GAAGGATGGGTGAAAGTGACGGAGGAGTACAGCGAGAGGCTGATGGGTTT


 


GGCTTGTAAACTTCTTGAGGTTTTGTCTGAAGCTATGGGGCTCGAGAAAG


 


AGGCACTCACCAATGCATGCGTCGATATGGACCAGAAAATAGTTGTTAAC


 


TATTACCCAAAATGCCCTCAGCCTGATCTAACCCTCGGGCTCAAGCGTCA


 


CACTGACCCTGGAACCATCACTTTGCTGCTCCAAGACCAAGTTGGTGGTT


 


TACAAGCCACACGAGACGATGGGAAGACATGGATTACAGTTCAGCCTGTT


 


GAAGGAGCTTTTGTTGTTAATCTTGGCGACCATGGTCACTATCTGAGCAA


 


CGGGAGGTTCAAGAACGCTGACCACCAGGCGGTGGTGAACTCCAACTCGA


 


GCAGACTATCAATAGCCACGTTCCAGAATCCGGCGCCGGAAGCAACCGTG


 


TATCCGCTTAAAGTGAGAGAAGGAGAGAAGCCGATCTTGGAGGAGCCAAT


 


TACGTTTGCGGAGATGTATAAGAGAAAGATGAGTAGAGATCTCGAGCTGG


 


CTCGCCTCAAGAAGCTGGCGAAAGAAGAGCATGACCACAAGGAAGCTGCC


 


AAGCCTCTAGACCAAATCATCGCTTAG


 


Brassica napus S3H homologous protein sequence


(SEQ ID NO: 27):


MAPGTLNELAGETKLNSKFVRDEDERPKVAYNEFSTEIPVISLAGIDDVG


 


EKRGEICRQIVEACENWGVFQVVDHGVDTSLVADMTRLARDFFALPPEEK


 


LKFDMSGGKKGGFIVSSHLQGESVQDWREIVTYFSYPVRNRDYSRWPTKP


 


EGWVKVTEEYSERLMGLACKLLEVLSEAMGLEKEALTNACVDMDQKIVVN


 


YYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATRDDGKTWITVQPV


 


EGAFVVNLGDHGHYLSNGRFKNADHQAVVNSNSSRLSIATFQNPAPEATV


 


YPLKVREGEKPILEEPITFAEMYKRKMSRDLELARLKKLAKEEHDHKEAA


 


KPLDQIIA


 


Solanum tuberosum (Potato) S3H homologous coding


cDNA sequence (SEQ ID NO: 28):


ATGGCTTCAACACTAACAGCTCTAGCTAATGAAAAGACCCTTCAAACAAG


 


TTTTATTAGGGATGAAGAAGAACGTCCAAAAGTGGCTTACAATAAATTTA


 


GTGACGAAATTCCAGTAATATCGTTGCAAGGTATTGATGATATTAATGGA


 


AGAAGAAGTGAAATATGTGAGAAAATTGTAAATGCTTGTGAAGATTGGGG


 


AGTTTTTCAGGTAATTGATCATGGGGCCGATGCTCAATTAATATCAGAAA


 


TGACAAAATTGGCTAAGGAATTTTTCGAATTGCCTCCTGACGAAAAGCTT


 


CGGTTTGACATGTCTGGTGGCAAGAAAGGCGGCTTTATTGTCTCAAGCCA


 


TTTACAGGGTGAAGTGGTTCAAGACTGGCGTGAAATAGTGACCTACTTTT


 


CTTACCCAATTCGAGCTAGAGACTACTCCAGATGGCCAGACAAACCACAA


 


GGCTGGATAGCTGTAACTGAGAAATACAGTGAAAAATTAATGGACTTGGC


 


TTGCAAATTATTAGAAGTATTATCAGAGGCAATGGGCTTAGAGAAAGAGG


 


CTTTAACCAAGGCATGTGTGGATATGGACCAAAAAGTAGTTGTCAATTTT


 


TACCCAAAGTGTCCAGAGCCTGACCTTACCCTTGGGCTGAAACGACATAC


 


TGATCCAGGAACCATCACCCTCTTGTTACAAGACCAAGTTGGTGGGCTTC


 


AAGCCACTAAAGATAATGGCAAAACTTGGATCACTGTTCAGCCCGTTGAA


 


GGCGCTTTTGTTGTTAATCTTGGTGATCATGGTCATTATTTGAGCAATGG


 


GAGGTTCAAGAATGCTGATCATCAAGCAGTTGTGAATTCGAATAGCAGCA


 


GATTATCGATAGCCACTTTTCAGAATCCAGCACCGGATGCAAAAGTGTAT


 


CCGTTAAAAATTAGGGAAGGAGAGAAGGCAATAATGGATGAGCCGATTAC


 


ATTTGCAGAAATGTACAGGAGGAAAATGAGTAAGGATCTTGAGCTTGCTA


 


GGCTCAAGAAACTGGCCAAGGAACAGACTGAAGAGGCCAAGTTGGAGTCC


 


AAGCCCATTGAGGAAATTCTTGCTTAA


 


Solanum tuberosum (Potato) S3H homologous protein


sequence (SEQ ID NO: 29):


MASTLTALANEKTLQTSFIRDEEERPKVAYNKFSDEIPVISLQGIDDING


 


RRSEICEKIVNACEDWGVFQVIDHGADAQLISEMTKLAKEFFELPPDEKL


 


RFDMSGGKKGGFIVSSHLQGEVVQDWREIVTYFSYPIRARDYSRWPDKPQ


 


GWIAVTEKYSEKLMDLACKLLEVLSEAMGLEKEALTKACVDMDQKVVVNF


 


YPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQATKDNGKTWITVQPVE


 


GAFVVNLGDHGHYLSNGRFKNADHQAVVNSNSSRLSIATFQNPAPDAKVY


 


PLKIREGEKAIMDEPITFAEMYRRKMSKDLELARLKKLAKEQTEEAKLES


 


KPIEEILA


 


Solanum lycopersicum (Tomato) S3H homologous


coding cDNA sequence (SEQ ID NO: 30):


ATGACAACAACAAGTGTTCTTTCTAGTGGATTCAACCACTCAACCCTCCC


 


TCAGTCTTACGTTCGACCTGAATCTCAAAGACCTTGCATGTCTGAAGTTG


 


TTGATAGCGACGATCTTGTCCCAGTCATTGATATGTCTTGTACTAATAGG


 


AACGTTATCGTTCATCAAATCGGTGAAGCTTGTCGTCTTTATGGGTTTTT


 


CCAGGTGATAAATCACGGTGTGTCGAAGAAGGTGATAGATGAAATGTTAG


 


GGGTAAGTCATGAATTTTTTAAGCTACCAGTTGAAGAAAAGATGAAATTG


 


TATTCTGATGATCCATCAAAAACTATGAGATTATCAACTAGTTTTAATGT


 


TAAGAAGGAAGCTGTTCATAATTGGAGAGATTATCTTAGGCTACATTGTT


 


ATCCTTTGGACAAATATGCCCCTGAATGGCCTTCTAATCCTCCTTCTTTC


 


AGGGAAATAGTAAGCAAATATTGCATGGAAGTTAGAGAGCTTGGATATAG


 


ATTGGAAGAAGCAATATCAGAGAGCTTAGGGCTTGAGAAGGATTGTATAA


 


AAAATGTGTTAGGTGAACAAGGACAACATATGGCTATCAATTTTTATCCT


 


CAGTGTCCACAACCTGAATTAACTTATGGGTTACCAGCCCATACAGATCC


 


AAATGCAATTACAATTCTTCTTCAAGATTTGCAAGTGGCTGGCCTTCAAG


 


TTCTTAAGGATGGAAAATGGCTATCTATTAAACCTCAGCCTAATGCCTTT


 


GTCATCAATCTTGGTGATCAATTGGAGGCGTTGAGTAATGGGAAGTATAA


 


AAGTATATGGCATAGAGCTATAGTGAATTCAGACAAAGCAAGGATGTCTG


 


TGGCTTCTTTCCTCTGTCCCAATGATTGTTCCATTATCAGTGCTCCAAAA


 


ACCTTAACTGAAGATGGATCTTCTGCAATTTATCGACATTTCACTTATGC


 


TGAATATTATGAAAAATTCTGGAGCAGGAATTTAGATCAGGAATATTGTT


 


TAGAACTTTTCAAGAACGATGGAACCTAG


 


Solanum lycopersicum (Tomato) S3H homologous


protein sequence (SEQ ID NO: 31):


MTTTSVLSSGFNHSTLPQSYVRPESQRPCMSEVVDSDDLVPVIDMSCTNR


 


NVIVHQIGEACRLYGFFQVINHGVSKKVIDEMLGVSHEFFKLPVEEKMKL


 


YSDDPSKTMRLSTSFNVKKEAVHNWRDYLRLHCYPLDKYAPEWPSNPPSF


 


REIVSKYCMEVRELGYRLEEAISESLGLEKDCIKNVLGEQGQHMAINFYP


 


QCPQPELTYGLPAHTDPNAITILLQDLQVAGLQVLKDGKWLSIKPQPNAF


 


VINLGDQLEALSNGKYKSIWHRAIVNSDKARMSVASFLCPNDCSIISAPK


 


TLTEDGSSAIYRHFTYAEYYEKFWSRNLDQEYCLELFKNDGT






In one embodiment, the transgenic plant has a reduced level of S3H and displays a precocious or premature leaf senescence phenotype.
In one embodiment, the plant is transformed with a nucleic acid construct comprising a nucleic acid molecule configured to silence S3H protein expression.
In one embodiment, the nucleic acid molecule is configured to silence a protein having the amino acid sequence of NxYPxCPxPxLxxGxxxHxDxxxxTxLLQD (SEQ ID NO: 1), where x can be any amino acid residue. In another embodiment, the nucleic acid construct is configured to silence a protein having the amino acid sequence selected from the group consisting of SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 17, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO: 23, SEQ ID NO: 25, SEQ ID NO: 27, SEQ ID NO: 29, and SEQ ID NO: 31.
In one embodiment, the transgenic plant has an increased level of S3H protein and displays a delayed leaf senescence phenotype. In this embodiment, the plant may be transformed with a nucleic acid construct configured to overexpress S3H protein.
Accordingly, the nucleic acid construct may encode a protein having the amino acid sequence of NxYPxCPxPxLxxGxxxHxDxxxxTxLLQD (SEQ ID NO: 1), wherein x can be any amino acid residue. The nucleic acid construct may also encode a protein having the amino acid sequence selected from the group consisting of SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 17, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO: 23, SEQ ID NO: 25, SEQ ID NO: 27, SEQ ID NO: 29, and SEQ ID NO: 31. An alignment of these sequences is shown in FIG. 14.
The nucleic acid molecule may include the nucleotide sequence of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 18, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 28, and SEQ ID NO: 30.
While activity in particular polypeptide or protein sequences has been identified, variants of those polypeptides are also contemplated and may also be used as described herein (e.g., to alter senescence and/or impart or enhance disease resistance). In some embodiments, the polypeptides of the invention comprise one or more (e.g., 1, 2, 3, 4, 5 or more) amino acid insertions, deletions, or modifications (e.g., substitution of one amino acid for another) compared to SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 17, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO: 23, SEQ ID NO: 25, SEQ ID NO: 27, SEQ ID NO: 29, and/or SEQ ID NO: 31 or are otherwise substantially identical (e.g., having a sequence at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or more identical) with the entire sequence of SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 17, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO: 23, SEQ ID NO: 25, SEQ ID NO: 27, SEQ ID NO: 29, and/or SEQ ID NO: 31. For example, polypeptides comprising or consisting of an amino acid sequence having one or more (e.g., 1, 2, 3, 4, 5, or more) conservative amino acid substitutions relative to such sequences, but retaining the function of such sequences (e.g., in altering leaf senescence and/or imparting or enhancing disease resistance) are encompassed. Further, nucleic acid molecules encoding such variants of the peptides of the present invention are also contemplated. Such nucleic acid molecules may have, for example, a nucleotide sequence at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or more identical) with the entire sequence of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 18, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 28, and/or SEQ ID NO: 30.
Components of nucleic acid constructs according to the present invention may be heterologous or exogenous. A polynucleotide sequence is “heterologous to” or “exogenous to” an organism or a second polynucleotide sequence if it is synthetic or originates from a foreign species, or, if from the same species, is modified from its original form. For example, a promoter operably linked to a heterologous coding sequence (or vice versa) refers to a coding sequence from a species different from that from which the promoter was derived, or, if from the same species, a coding sequence which is not naturally associated with the promoter (e.g. a genetically engineered coding sequence or an allele from a different ecotype or variety).
Methods of producing recombinant nucleic acids for purposes of, e.g., making transgenic plants are well-known. Recombinant molecules can be introduced into cells via transformation, particularly transduction, conjugation, mobilization, or electroporation. The DNA sequences are cloned into the vector using standard cloning procedures in the art, as described by Sambrook et al., Molecular Cloning: A Laboratory Manual, Second Edition, Cold Spring Harbor, N.Y., Cold Spring Harbor Press (1989), and Ausubel et al., Current Protocols in Molecular Biology, New York, N.Y., John Wiley & Sons (1989), which are hereby incorporated by reference in their entirety.
In preparing a nucleic acid vector for expression, the various nucleic acid sequences may normally be inserted or substituted into a bacterial plasmid. Any convenient plasmid may be employed, which will be characterized by having a bacterial replication system, a marker which allows for selection in a bacterium, and generally one or more unique, conveniently located restriction sites. Numerous plasmids, referred to as transformation vectors, are available for plant transformation. The selection of a vector will depend on the preferred transformation technique. A variety of vectors are available for stable transformation using Agrobacterium tumefaciens, a soilborne bacterium that causes crown gall. Crown gall are characterized by tumors or galls that develop on the lower stem and main roots of the infected plant. These tumors are due to the transfer and incorporation of part of the bacterium plasmid DNA into the plant chromosomal DNA. This transfer DNA (“T-DNA”) is expressed along with the normal genes of the plant cell. The plasmid DNA, pTi, or Ti-DNA, for “tumor inducing plasmid,” contains the vir genes necessary for movement of the T-DNA into the plant. The T-DNA carries genes that encode proteins involved in the biosynthesis of plant regulatory factors, and bacterial nutrients (opines). The T-DNA is delimited by two 25 bp imperfect direct repeat sequences called the “border sequences.” By removing the oncogene and opine genes, and replacing them with a gene of interest, it is possible to transfer foreign DNA into the plant without the formation of tumors or the multiplication of Agrobacterium tumefaciens (Fraley et al., “Expression of Bacterial Genes in Plant Cells,” Proc. Nat'l Acad. Sci. 80:4803-4807 (1983), which is hereby incorporated by reference in its entirety).
Further improvement of this technique led to the development of the binary vector system (Bevan, “Binary Agrobacterium Vectors for Plant Transformation,” Nucleic Acids Res. 12:8711-8721 (1984), which is hereby incorporated by reference in its entirety). In this system, all the T-DNA sequences (including the borders) are removed from the pTi, and a second vector containing T-DNA is introduced into Agrobacterium tumefaciens. This second vector has the advantage of being replicable in E. coli as well as A. tumefaciens, and contains a multiclonal site that facilitates the cloning of a transgene. An example of a commonly-used vector is pBin19 (Frisch et al., “Complete Sequence of the Binary Vector Bin19,” Plant Molec. Biol. 27:405-409 (1995), which is hereby incorporated by reference in its entirety). Any appropriate vectors now known or later described for genetic transformation are suitable for use with the present invention.
U.S. Pat. No. 4,237,224 to Cohen and Boyer, which is hereby incorporated by reference in its entirety, describes the production of expression systems in the form of recombinant plasmids using restriction enzyme cleavage and ligation with DNA ligase. These recombinant plasmids are then introduced by means of transformation and replicated in unicellular cultures including prokaryotic organisms and eukaryotic cells grown in tissue culture.
Certain “control elements” or “regulatory sequences” are also incorporated into the vector-construct. These include non-translated regions of the vector, promoters, and 5′ and 3′ untranslated regions which interact with host cellular proteins to carry out transcription and translation. Such elements may vary in their strength and specificity. Depending on the vector system and host utilized, any number of suitable transcription and translation elements, including constitutive and inducible promoters, may be used. Tissue-specific and organ-specific promoters can also be used.
A constitutive promoter is a promoter that directs expression of a gene throughout the development and life of an organism. Examples of some constitutive promoters that are widely used for inducing expression of transgenes include the nopaline synthase (NOS) gene promoter, from Agrobacterium tumefaciens (U.S. Pat. No. 5,034,322 to Rogers et al., which is hereby incorporated by reference in its entirety), the cauliflower mosaic virus (CaMV) 35S and 19S promoters (U.S. Pat. No. 5,352,605 to Fraley et al., which is hereby incorporated by reference in its entirety), those derived from any of the several actin genes, which are known to be expressed in most cells types (U.S. Pat. No. 6,002,068 to Privalle et al., which is hereby incorporated by reference in its entirety), and the ubiquitin promoter, which is a gene product known to accumulate in many cell types.
An inducible promoter is a promoter that is capable of directly or indirectly activating transcription of one or more DNA sequences or genes in response to an inducer. In the absence of an inducer, the DNA sequences or genes will not be transcribed. The inducer can be a chemical agent, such as a metabolite, growth regulator, herbicide, or phenolic compound, or a physiological stress directly imposed upon the plant such as cold, heat, salt, toxins, or through the action of a pathogen or disease agent such as a virus or fungus. A plant cell containing an inducible promoter may be exposed to an inducer by externally applying the inducer to the cell or plant such as by spraying, watering, heating, or by exposure to the operative pathogen. An example of an appropriate inducible promoter is a glucocorticoid-inducible promoter (Schena et al., “A Steroid-Inducible Gene Expression System for Plant Cells,” Proc. Natl. Acad. Sci. 88:10421-5 (1991), which is hereby incorporated by reference in its entirety). Expression of the transgene-encoded protein is induced in the transformed plants when the transgenic plants are brought into contact with nanomolar concentrations of a glucocorticoid, or by contact with dexamethasone, a glucocorticoid analog (Schena et al., “A Steroid-Inducible Gene Expression System for Plant Cells,” Proc. Natl. Acad. Sci. USA 88:10421-5 (1991); Aoyama et al., “A Glucocorticoid-Mediated Transcriptional Induction System in Transgenic Plants,” Plant J. 11:605-612 (1997); and McNellis et al., “Glucocorticoid-Inducible Expression of a Bacterial Avirulence Gene in Transgenic Arabidopsis Induces Hypersensitive Cell Death,” Plant J. 14(2):247-57 (1998), which are hereby incorporated by reference in their entirety). In addition, inducible promoters include promoters that function in a tissue specific manner to regulate the gene of interest within selected tissues of the plant. Examples of such tissue specific or developmentally regulated promoters include seed, flower, fruit, or root specific promoters as are well known by those of ordinary skill in the art (U.S. Pat. No. 5,750,385 to Shewmaker et al., which is hereby incorporated by reference in its entirety).
A number of tissue- and organ-specific promoters have been developed for use in genetic engineering of plants (Potenza et al., “Targeting Transgene Expression in Research, Agricultural, and Environmental Applications: Promoters Used in Plant Transformation,” In Vitro Cell. Dev. Biol. Plant 40:1-22 (2004), which is hereby incorporated by reference in its entirety). Examples of such promoters include those that are floral-specific (Annadana et al., “Cloning of the Chrysanthemum UEP1 Promoter and Comparative Expression in Florets and Leaves of Dendranthema grandiflora,” Transgenic Res. 11:437-445 (2002), which is hereby incorporated by reference in its entirety), seed-specific (Kluth et al., “5′ Deletion of a gbss1 Promoter Region Leads to Changes in Tissue and Developmental Specificities,” Plant Mol. Biol. 49:669-682 (2002), which is hereby incorporated by reference in its entirety), root-specific (Yamamoto et al., “Characterization of cis-acting Sequences Regulating Root-Specific Gene Expression in Tobacco,” Plant Cell 3:371-382 (1991), which is hereby incorporated by reference in its entirety), fruit-specific (Fraser et al., “Evaluation of Transgenic Tomato Plants Expressing an Additional Phytoene Synthase in a Fruit-Specific Manner,” Proc. Natl. Acad. Sci. USA 99:1092-1097 (2002), which is hereby incorporated by reference in its entirety), and tuber/storage organ-specific (Visser et al., “Expression of a Chimaeric Granule-Bound Starch Synthase-GUS Gene in Transgenic Potato Plants,” Plant Mol. Biol. 17:691-699 (1991), which is hereby incorporated by reference in its entirety). Targeted expression of an introduced gene (transgene) is necessary when expression of the transgene could have detrimental effects if expressed throughout the plant. On the other hand, silencing a gene throughout a plant could also have negative effects. However, this problem could be avoided by localizing the silencing to a region by a tissue-specific promoter.
Nucleic acid constructs of the present invention include an operable 3′ regulatory region, selected from among those which are capable of providing correct transcription termination and polyadenylation of mRNA for expression in the host cell of choice, operably linked to a nucleic acid molecule configured to silence BBTV. A number of 3′ regulatory regions are known to be operable in plants. Exemplary 3′ regulatory regions include, without limitation, the nopaline synthase (“nos”) 3′ regulatory region (Fraley et al., “Expression of Bacterial Genes in Plant Cells,” Proc. Nat'l Acad. Sci. USA 80:4803-4807 (1983), which is hereby incorporated by reference in its entirety) and the cauliflower mosaic virus (“CaMV”) 3′ regulatory region (Odell et al., “Identification of DNA Sequences Required for Activity of the Cauliflower Mosaic Virus 35S Promoter,” Nature 313(6005):810-812 (1985), which is hereby incorporated by reference in its entirety). Virtually any 3′ regulatory region known to be operable in plants would be suitable for use in conjunction with the present invention.
The different components described supra can be ligated together to produce the expression systems which contain the nucleic acid constructs of the present invention, using well known molecular cloning techniques as described in Sambrook et al., Molecular Cloning: A Laboratory Manual, Second Edition Cold Spring Harbor, N.Y., Cold Spring Harbor Press (1989), and Ausubel et al. Current Protocols in Molecular Biology, New York, N.Y., John Wiley & Sons (1989), which are hereby incorporated by reference in their entirety.
Once the nucleic acid construct of the present invention has been prepared, it is ready to be incorporated into a host cell. Accordingly, another aspect of the present invention relates to a recombinant host cell containing one or more of the nucleic acid constructs of the present invention. Basically, this method is carried out by transforming a host cell with a nucleic acid construct of the present invention under conditions effective to achieve transcription of the nucleic acid molecule in the host cell. This is achieved with standard cloning procedures known in the art, such as described by Sambrook et al., Molecular Cloning: A Laboratory Manual, Second Edition, Cold Springs Laboratory, Cold Springs Harbor, N.Y. (1989), which is hereby incorporated by reference in its entirety. Suitable host cells include, but are not limited to, bacteria, virus, yeast, mammalian cells, insect, plant, and the like. Preferably the host cells are either a bacterial cell or a plant cell. Methods of transformation may result in transient or stable expression of the nucleic acid under control of the promoter. Preferably, a nucleic acid construct of the present invention is stably inserted into the genome of the recombinant plant cell as a result of the transformation, although transient expression can serve an important purpose.
Plant tissue suitable for transformation includes leaf tissue, root tissue, meristems, zygotic and somatic embryos, callus, protoplasts, tassels, pollen, embryos, anthers, and the like. The means of transformation chosen is that most suited to the tissue to be transformed.
Transient expression in plant tissue can be achieved by particle bombardment (Klein et al., “High-Velocity Microprojectiles for Delivering Nucleic Acids Into Living Cells,” Nature 327:70-73 (1987), which is hereby incorporated by reference in its entirety), also known as biolistic transformation of the host cell, as disclosed in U.S. Pat. Nos. 4,945,050; 5,036,006; and 5,100,792, all to Sanford et al., and in Emerschad et al., “Somatic Embryogenesis and Plant Development from Immature Zygotic Embryos of Seedless Grapes (Vitis vinifera),” Plant Cell Reports 14:6-12 (1995), which are hereby incorporated by reference in their entirety.
In particle bombardment, tungsten or gold microparticles (1 to 2 μm in diameter) are coated with the DNA of interest and then bombarded at the tissue using high pressure gas. In this way, it is possible to deliver foreign DNA into the nucleus and obtain a temporal expression of the gene under the current conditions of the tissue. Biologically active particles (e.g., dried bacterial cells containing the vector and heterologous DNA) can also be propelled into plant cells. Other variations of particle bombardment, now known or hereafter developed, can also be used.
An appropriate method of stably introducing the nucleic acid construct into plant cells is to infect a plant cell with Agrobacterium tumefaciens or Agrobacterium rhizogenes previously transformed with the nucleic acid construct of the present invention. As described supra, the Ti (or RI) plasmid of Agrobacterium enables the highly successful transfer of a foreign nucleic acid molecule into plant cells. A variation of Agrobacterium transformation uses vacuum infiltration in which whole plants are used (Senior, “Uses of Plant Gene Silencing,” Biotechnology and Genetic Engineering Reviews 15:79-119 (1998), which is hereby incorporated by reference in its entirety).
Yet another method of introduction is fusion of protoplasts with other entities, either minicells, cells, lysosomes, or other fusible lipid-surfaced bodies (Fraley et al., “Liposome-mediated Delivery of Tobacco Mosaic Virus RNA Into Tobacco Protoplasts: A Sensitive Assay for Monitoring Liposome-protoplast Interactions,” Proc. Natl. Acad. Sci. USA 79:1859-63 (1982), which is hereby incorporated by reference in its entirety). The nucleic acid molecule may also be introduced into the plant cells by electroporation (Fromm et al., “Expression of Genes Transferred into Monocot and Dicot Plant Cells by Electroporation,” Proc. Natl. Acad. Sci. USA 82:5824 (1985), which is hereby incorporated by reference in its entirety). In this technique, plant protoplasts are electroporated in the presence of plasmids containing the expression cassette. Electrical impulses of high field strength reversibly permeabilize biomembranes allowing the introduction of the plasmids. Electroporated plant protoplasts reform the cell wall, divide, and regenerate. Other methods of transformation include polyethylene-mediated plant transformation, micro-injection, physical abrasives, and laser beams (Senior, “Uses of Plant Gene Silencing,” Biotechnology and Genetic Engineering Reviews 15:79-119 (1998), which is hereby incorporated by reference in its entirety). The precise method of transformation is not critical to the practice of the present invention. Any method that results in efficient transformation of the host cell of choice is appropriate for practicing the present invention.
Yet a further method for introduction is by use of known techniques for genome editing or alteration. Such techniques for targeted genomic insertion involve, for example, inducing a double stranded DNA break precisely at one or more targeted genetic loci followed by integration of a chosen transgene or nucleic acid molecule (or construct) during repair. Such techniques or systems include, for example, zinc finger nucleases (“ZFNs”) (Urnov et al., “Genome Editing with Engineered Zinc Finger Nucleases,” Nat Rev Genet. 11: 636-646 (2010), which is hereby incorporated by reference in its entirety), transcription activator-like effector nucleases (“TALENs”) (Joung & Sander, “TALENs: A Widely Applicable Technology for Targeted Genome Editing,” Nat Rev Mol Cell Biol. 14: 49-55 (2013), which is hereby incorporated by reference in its entirety), clustered regularly interspaced short palindromic repeat (“CRISPR”)-associated endonucleases (e.g., CRISPR/CRISPR-associated (“Cas”) 9 systems) (Wiedenheft et al., “RNA-Guided Genetic Silencing Systems in Bacteria and Archaea,” Nat 482:331-338 (2012); Zhang et al., “Multiplex Genome Engineering Using CRISPR/Cas Systems,” Science 339(6121): 819-23 (2013); and Gaj et al., “ZFN, TALEN, and CRISPR/Cas-based Methods for Genome Engineering,” Cell 31(7):397-405 (2013), each of which is hereby incorporated by reference in its entirety).
After transformation, the transformed plant cells must be regenerated. Plant regeneration from cultured protoplasts is described in Evans et al., Handbook of Plant Cell Cultures, Vol. 1, New York, N.Y., MacMillan Publishing Co. (1983); Vasil, ed., Cell Culture and Somatic Cell Genetics of Plants, Vol. I (1984) and Vol. III (1986), Orlando, Acad. Press; and Fitch et al., “Somatic Embryogenesis and Plant Regeneration from Immature Zygotic Embryos of Papaya (Carica papaya L.),” Plant Cell Rep. 9:320 (1990), which are hereby incorporated by reference in their entirety.
Means for regeneration varies from species to species of plants, but generally a suspension of transformed protoplasts or a petri plate containing explants is first provided. Callus tissue is formed and shoots may be induced from callus and subsequently rooted. Alternatively, embryo formation can be induced in the callus tissue. These embryos germinate as natural embryos to form plants. The culture media will generally contain various amino acids and hormones, such as auxin and cytokinins Efficient regeneration will depend on the medium, on the genotype, and on the history of the culture. If these three variables are controlled, then regeneration is usually reproducible and repeatable.
Preferably, transformed cells are first identified using a selection marker simultaneously introduced into the host cells along with the nucleic acid construct of the present invention. Suitable selection markers include, without limitation, markers encoding for antibiotic resistance, such as the neomycin phosphotransferae II (“nptII”) gene which confers kanamycin resistance (Fraley et al., “Expression of Bacterial Genes in Plant Cells,” Proc. Natl. Acad. Sci. USA 80:4803-4807 (1983), which is hereby incorporated by reference in its entirety), and the genes which confer resistance to gentamycin, G418, hygromycin, streptomycin, spectinomycin, tetracycline, chloramphenicol, and the like. Cells or tissues are grown on a selection medium containing the appropriate antibiotic, whereby generally only those transformants expressing the antibiotic resistance marker continue to grow. Other types of markers are also suitable for inclusion in the expression cassette of the present invention. For example, a gene encoding for herbicide tolerance, such as tolerance to sulfonylurea is useful, or the dhfr gene, which confers resistance to methotrexate (Bourouis et al., EMBO J. 2:1099-1104 (1983), which is hereby incorporated by reference in its entirety). Similarly, “reporter genes,” which encode for enzymes providing for production of an identifiable compound are suitable. The most widely used reporter gene for gene fusion experiments has been uidA, a gene from Escherichia coli that encodes the β-glucuronidase protein, also known as GUS (Jefferson et al., “GUS Fusions: β Glucuronidase as a Sensitive and Versatile Gene Fusion Marker in Higher Plants,” EMBO J. 6:3901-3907 (1987), which is hereby incorporated by reference in its entirety). Similarly, enzymes providing for production of a compound identifiable by luminescence, such as luciferase, are useful. The selection marker employed will depend on the target species; for certain target species, different antibiotics, herbicide, or biosynthesis selection markers are preferred.
Plant cells and tissues selected by means of an inhibitory agent or other selection marker are then tested for the acquisition of the transgene (Sambrook et al., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor, N.Y., Cold Spring Harbor Press (1989), which is hereby incorporated by reference in its entirety).
In one embodiment, the transgenic plant is transformed with a bacterial artificial chromosome (“BAC”). A BAC is a cloning vector derived from the naturally occurring F factor of Escherichia coli. BACs can accept large inserts of a DNA sequence. BACs have a propensity for coming together to form contiguous stretches of DNA. A BAC “assembles” to a contig based on sequence alignment, if the BAC is sequenced, or via the alignment of its BAC fingerprint to the fingerprints of other BACs.
Accordingly, one aspect of the present invention relates to a plant or plant seed transformed with one or more nucleic acid constructs described herein. The present invention also encompasses the whole plant, or a component part of a plant, including shoot vegetative organs/structures (e.g. leaves, stems and tubers), roots, flowers and floral organs/structures (e.g. bracts, sepals, petals, stamens, carpels, anthers and ovules), seed (including embryo, endosperm, and seed coat) and fruit (the mature ovary), plant tissue (e.g. vascular tissue, ground tissue, and the like) and cells (e.g. guard cells, egg cells, trichomes and the like), and progeny of same.
Suitable plants may be selected from the group consisting of rice, corn, soybean, canola, potato, wheat, mung bean, alfalfa, barley, rye, cotton, sunflower, peanut, sweet potato, bean, pea, chicory, lettuce, endive, cabbage, brussel sprout, beet, parsnip, turnip, cauliflower, broccoli, radish, spinach, onion, garlic, eggplant, pepper, celery, carrot, squash, pumpkin, zucchini, cucumber, apple, pear, melon, citrus, strawberry, grape, raspberry, pineapple, tobacco, tomato, sorghum, sugarcane, banana, Arabidopsis thaliana, Saintpaulia, petunia, pelargonium, poinsettia, chrysanthemum, carnation, crocus, marigold, daffodil, pine, Medicago truncatula, Sandersonia aurantiaca, Populus trichocarpa, and zinnia. Suitable plants as described herein may also be selected from Arabidopsis thaliana, Populus trichocarpa, Zea mays, Sorghum bicolor, Oryza sativa (rice), Hordeum vulgare (common barley), Triticum aestivum (wheat), Nicotiana tabacum, petunia, Glycine max (Soybean), cotton, Malus domestica (Apple), Brassica napus, Solanum tuberosum (Potato), or Solanum lycopersicum (Tomato).
Accordingly, yet another aspect of the present invention relates to a method for promoting premature or precocious leaf senescence in a plant. This methods involves providing a transgenic plant or plant seed transformed with a nucleic acid construct effective in silencing expression of a S3H protein capable of causing leaf senescence in a plant; and growing the transgenic plant or the plant grown from the transgenic plant seed under conditions effective to promote premature or precocious leaf senescence in the transgenic plant or the plant grown from the transgenic plant seed.
In one embodiment, the plant is transformed. In another embodiment, a plant seed is transformed.
In one embodiment, the nucleic acid construct includes a nucleic acid molecule configured to silence S3H protein expression; a 5′ DNA promoter sequence; and a 3′ terminator sequence, where the nucleic acid molecule, the promoter, and the terminator are operatively coupled to permit expression of the nucleic acid molecule.
Suitable nucleic acid molecules and corresponding S3H protein sequence for use in accordance with the present invention are described above.
In one embodiment, the nucleic acid molecule is positioned in the nucleic acid construct to result in suppression or interference of endogenous mRNA encoding the S3H protein.
In one embodiment, the nucleic acid molecule includes an antisense nucleic acid molecule to a nucleic acid molecule encoding an S3H protein or a portion thereof.
In one aspect of the present invention, the nucleic acid construct results in suppression or interference of S3H protein expression by the nucleic acid molecule of the construct containing a dominant negative mutation and encoding a non-functional S3H protein.
In another aspect of the present invention, the nucleic acid construct results in interference of S3H protein expression by sense or co-suppression in which the nucleic acid molecule of the construct (e.g., that encoding S3H or a fragment thereof) is in a sense (5′→3′) orientation. Co-suppression has been observed and reported in many plant species and may be subject to a transgene dosage effect or, in another model, an interaction of endogenous and transgene transcripts that results in aberrant mRNAs (Senior, “Uses of Plant Gene Silencing,” Biotechnology and Genetic Engineering Reviews 15:79-119 (1998); Waterhouse et al., “Exploring Plant Genomes by RNA-Induced Gene Silencing,” Nature Review: Genetics 4: 29-38 (2003), which are hereby incorporated by reference in their entirety). A construct with the nucleic acid molecule (or fragment thereof) in the sense orientation may also give sequence specificity to RNA silencing when inserted into a vector along with a construct of both sense and antisense nucleic acid orientations as described infra (Wesley et al., “Construct Design for Efficient, Effective and High-Throughput Gene Silencing in Plants,” Plant Journal 27(6) 581-590 (2001), which is hereby incorporated by reference in its entirety).
In another embodiment of the present invention, the nucleic acid construct results in interference of S3H protein expression by the use of antisense suppression in which the nucleic acid molecule of the construct (e.g., that encoding S3H or a fragment thereof) is an antisense (3′→5′) orientation. The use of antisense RNA to down-regulate the expression of specific plant genes is well known (van der Krol et al., Nature, 333:866-869 (1988) and Smith et al., Nature, 334:724-726 (1988), which are hereby incorporated by reference in their entirety). Antisense nucleic acids are DNA or RNA molecules that are complementary to at least a portion of a specific mRNA molecule (Weintraub, “Antisense RNA and DNA,” Scientific American 262:40 (1990), which is hereby incorporated by reference in its entirety). In the target cell, the antisense nucleic acids hybridize to a target nucleic acid and interfere with transcription, and/or RNA processing, transport, translation, and/or stability. The overall effect of such interference with the target nucleic acid function is the disruption of protein expression (Baulcombe, “Mechanisms of Pathogen-Derived Resistance to Viruses in Transgenic Plants,” Plant Cell 8:1833-44 (1996); Dougherty, et al., “Transgenes and Gene Suppression: Telling us Something New?,” Current Opinion in Cell Biology 7:399-05 (1995); Lomonossoff, “Pathogen-Derived Resistance to Plant Viruses,” Ann. Rev. Phytopathol. 33:323-43 (1995), which are hereby incorporated by reference in their entirety). Accordingly, one aspect of the present invention involves a nucleic acid construct which contains an antisense nucleic acid molecule to a nucleic acid molecule encoding an S3H protein (or fragment thereof).
Such sense and antisense molecules could be readily generated based on the nucleotide sequences of S3H described above (e.g., SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 18, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 28, and/or SEQ ID NO: 30).
RNA interference of S3H protein expression is also achieved in the present invention by the generation of double-stranded RNA (“dsRNA”) through the use of inverted-repeats, segments of gene-specific sequences oriented in both sense and antisense orientations. In one embodiment of this aspect of the present invention, sequences in the sense and antisense orientations are linked by a third segment, and inserted into a suitable expression vector having the appropriate 5′ and 3′ regulatory nucleotide sequences operably linked for transcription. The expression vector having the modified nucleic acid molecule is then inserted into a suitable host cell or subject. In the present invention, the third segment linking the two segments of sense and antisense orientation may be any nucleotide sequence such as a fragment of the β-glucuronidase (“GUS”) gene. In another embodiment of this aspect of the present invention, a functional (splicing) intron of the s3h gene may be used for the third (linking) segment, or, in yet another aspect of the present invention, other nucleotide sequences without complementary components in the s3h gene may be used to link the two segments of sense and antisense orientation (Chuang et al., “Specific and Heritable Genetic Interference by Double-Stranded RNA in Arabidopsis thaliana,” Proc. Nat'l Academy of Sciences USA 97(9):4985-4990 (2000); Smith et al., “Total Silencing by Intron-Spliced Hairpin RNAs,” Nature 407:319-320 (2000); Waterhouse et al., “Exploring Plant Genomes by RNA-Induced Gene Silencing,” Nature Review: Genetics 4:29-38 (2003); Wesley et al., “Construct Design for Efficient, Effective and High-Throughput Gene Silencing in Plants,” Plant Journal 27(6):581-590 (2001), which are hereby incorporated by reference in their entirety). In any of the embodiments with inverted repeats of S3H protein, the sense and antisense segments may be oriented either head-to-head or tail-to-tail in the construct.
Another aspect of the present invention involves using hairpin RNA (“hpRNA”) which may also be characterized as dsRNA. This involves RNA hybridizing with itself to form a hairpin structure that comprises a single-stranded loop region and a base-paired stem. Though a linker may be used between the inverted repeat segments of sense and antisense sequences to generate hairpin or double-stranded RNA, the use of intron-free hpRNA can also be used to achieve silencing of S3H protein expression.
Alternatively, in another aspect of the present invention, a plant may be transformed with constructs encoding both sense and antisense orientation molecules having separate promoters and no third segment linking the sense and antisense sequences (Chuang et al., “Specific and Heritable Genetic Interference by Double-Stranded RNA in Arabidopsis thaliana,” Proc. Nat'l Academy of Sciences USA 97(9):4985-4990 (2000); Waterhouse et al., “Exploring Plant Genomes by RNA-Induced Gene Silencing,” Nat Rev Genet. 4:29-38 (2003); Wesley et al., “Construct Design for Efficient, Effective and High-Throughput Gene Silencing in Plants,” Plant Journal 27(6):581-590 (2001), which are hereby incorporated by reference in their entirety).
Altering expression (e.g., inhibition of, or interference with, endogenous expression) of S3H can also be accomplished using known techniques for targeted alteration of genes, such as ZFNs, TALENs, CRISPR-associated endonucleases, which are described above.
Another aspect of the present invention relates to a method for delaying leaf senescence in a plant. This method involves transforming a plant cell with a nucleic acid molecule encoding a S3H capable of causing leaf senescence in a plant operably associated with a promoter to obtain a transformed plant cell, where expression of the nucleic acid molecule in the plant cell causes delayed leaf senescence; and regenerating a plant from the transformed plant cell under conditions effective to delay leaf senescence in the plant.
In one embodiment, such transforming includes transforming the plant cell with a nucleic acid construct comprising the nucleic acid molecule; a 5′ heterologous DNA promoter sequence; and a 3′ terminator sequence, where the nucleic acid molecule, the DNA promoter sequence, and the terminator sequence are operatively coupled to permit transcription of the nucleic acid molecule.
In one embodiment, the method involves regenerating a plant from the transformed plant cell and inducing the promoter under conditions effective to cause delayed leaf senescence in the plant.
Yet another aspect of the present invention relates to a method of making a mutant plant having a decreased level of S3H protein compared to that of a non-mutant plant, where the mutant plant displays a premature or precocious leaf senescence phenotype relative to a non-mutant plant. This method involves providing at least one cell of a non-mutant plant containing a gene encoding a functional S3H protein and treating the at least one cell of a non-mutant plant under conditions effective to inactivate said gene, thereby yielding at least one mutant plant cell containing an inactivated S3H protein encoding gene. This method also involves propagating the at least one mutant plant cell into a mutant plant, where the mutant plant has a decreased level of S3H protein compared to that of the non-mutant plant and displays a premature or precocious leaf senescence phenotype relative to a non-mutant plant.
In one embodiment, this method involves providing at least one cell of a non-mutant plant containing a gene encoding a functional S3H protein. Next, the at least one cell of a non-mutant plant is treated under conditions effective to inactivate the gene, thereby yielding at least one mutant plant cell containing an inactivated S3H gene. The at least one mutant plant cell is then propagated into a mutant plant, where the mutant plant has a decreased level of S3H protein compared to that of the non-mutant plant and displays a delayed leaf senescence phenotype relative to a non-mutant plant.
In other embodiments of this method of making a mutant plant, the functional S3H protein can be any S3H protein from a wide variety of plants as described herein supra.
In another embodiment of this method of making a mutant plant, the treating step involves subjecting the at least one cell of the non-mutant plant to a chemical mutagenizing agent under conditions effective to yield at least one mutant plant cell containing an inactive S3H gene. Suitable chemical mutagenizing agents can include, for example, ethylmethanesulfonate.
In another embodiment of this method of making a mutant plant, the treating step involves subjecting the at least one cell of the non-mutant plant to a radiation source under conditions effective to yield at least one mutant plant cell containing an inactive S3H gene. Suitable radiation sources can include, for example, sources that are effective in producing ultraviolet rays, gamma rays, or fast neutrons.
In another embodiment of this method of making a mutant plant, the treating step involves inserting an inactivating nucleic acid molecule into the gene encoding the functional S3H protein or its promoter under conditions effective to inactivate the gene. Suitable inactivating nucleic acid molecules can include, for example, a transposable element. Examples of such transposable elements include, but are not limited to, an Activator (Ac) transposon, a Dissociator (Ds) transposon, or a Mutator (Mu) transposon.
In yet another embodiment of this method of making a mutant plant, the treating step involves subjecting the at least one cell of the non-mutant plant to Agrobacterium transformation under conditions effective to insert an Agrobacterium T-DNA sequence into the gene, thereby inactivating the gene. Suitable Agrobacterium T-DNA sequences can include, for example, those sequences that are carried on a binary transformation vector of pAC106, pAC161, pGABI1, pADIS1, pCSA110, pDAP101, derivatives of pBIN19, or pCAMBIA plasmid series.
In still another aspect of this method of making a mutant plant, the treating step involves subjecting the at least one cell of the non-mutant plant to site-directed mutagenesis of the S3H gene or its promoter under conditions effective to yield at least one mutant plant cell containing an inactive S3H gene. The treating step can also involve mutagenesis by homologous recombination of the S3H gene or its promoter, targeted deletion of a portion of the S3H gene sequence or its promoter, and/or targeted insertion of a nucleic acid sequence into the S3H gene or its promoter. The various plants that can be used in this method are the same as those described supra with respect to the transgenic plants and mutant plants. Other embodiments of the present invention relate to mutant plants produced by this method, as well as mutant plant seeds produced by growing the mutant plant under conditions effective to cause the mutant plant to produce seed.
Accordingly, another aspect of the present invention relates to a mutant plant comprising an inactivated gene encoding S3H protein, where the mutant plant displays a premature or precocious leaf senescence phenotype, relative to a non-mutant plant.
Another aspect of the present invention relates to a method of inducing or promoting pathogen resistance in plants. This method involves providing a transgenic plant or plant seed transformed with a nucleic acid construct effective in silencing expression of a S3H protein capable of causing leaf senescence in a plant; and growing the transgenic plant or the plant grown from the transgenic plant seed under conditions effective to induce or promote pathogen resistance in the transgenic plant or the plant grown from the transgenic plant seed.
In one embodiment, a transgenic plant is provided. In another embodiment, a transgenic plant seed is provided.
Imparting disease or pathogen resistance or enhancing disease or pathogen resistance refers to an increase in the ability of a plant to prevent pathogen infection or pathogen-induced symptoms. Pathogen resistance may be increased compared to, for example, an unmodified or non-transgenic plant. Pathogen resistance can be increased resistance relative to a particular pathogen species or genus or can be increased resistance to all pathogens (e.g., systemic acquired resistance).
A further aspect of the present invention that is contemplated relates to a method of identifying a candidate plant suitable for breeding that displays a delayed senescence phenotype and or enhanced pathogen resistance. This method involves providing a candidate plant; analyzing the candidate plant for the presence, in its genome, of a gene encoding an S3H protein; identifying, based on said analyzing, a candidate plant suitable for breeding that includes in its genome, a gene encoding an S3H protein; and breeding the identified plant with at least one other plant.
In one embodiment, analyzing the candidate plant for the presence, in its genome, of a gene encoding an S3H protein involves isolating genomic DNA from the plant, germplasm, pollen, or seed of the plant; analyzing genomic DNA from the plant, germplasm, pollen, or seed of the plant for the presence of the gene encoding the S3H protein; and detecting the gene encoding the S3H protein.
In one embodiment, the breeding involves crossing, making hybrids, backcrossing, self-crossing, double haploid breeding, and/or combinations thereof.
In one embodiment, a transgenic plant transformed with a nucleic acid molecule that encodes an S3H protein is provided as the candidate plant. In one embodiment, providing the transgenic plant involves transforming a plant or plant seed with a nucleic acid construct according to the present invention and growing the transgenic plant or a plant grown from the transgenic plant seed under conditions effective to express the nucleic acid molecule in the transgenic plant or said plant grown from the transgenic plant seed.
EXAMPLES
Example 1—Plant Materials and Growth Conditions
Arabidopsis thaliana ecotype Col-0 was used as WT for all experiments. The T-DNA insertion line SALK_059907 was obtained from Arabidopsis Biological Resource Center (ABRC). Seeds were sown on Petri dishes containing MS with 0.7% w/v phytoagar and appropriate antibiotics and kept at 4° C. for 2 days before being moved to a growth chamber. Seedlings with two true leaves were transplanted to Cornell mix soils (3 parts peat moss: 2 parts vermiculite: 1 part perlite). WT, mutant and/or transgenic plants were grown side by side in the same tray to minimize possible variation of growth conditions. Plants were grown in 22° C. growth chamber with 60% relative humidity, under constant light (120 μmol m2 sec−1 light from a mixture of fluorescent and incandescent bulbs).
Example 2—Identification of T-DNA Insertion s3h Plants
Gene-specific primers G2563, G2564 (all the primers used in this research are listed in FIG. 1E) and T-DNA left border primer G2325 (LB1) were used to identify homozygous mutant plants of s3h.
Example 3—Chemicals and SA Treatment
All chemicals used in this research were purchased from Sigma-Aldrich (St. Louis, Mo.), unless otherwise stated. Col-0 plants (25 DAG) were sprayed with 5 mM SA in 0.005% Silwet L-77 (Lehele Seeds, Tex.) or only 0.005% Silwet L-77 (mock). All rosette leaves of individual plants for RNA extraction were collected at different time points after the spray.
Example 4—Constructs
(i) The coding sequence of S3H, which was PCR amplified with S3H-specific primers G2563 and G2564, was cloned into pGEM-T easy vector (Promega, Madison, Wis.), released with HindIII and PstI, and subcloned to the binary vector pGL800 (Zhou et al., “An Arabidopsis Mitogen-Activated Protein Kinase Cascade, MKK9-MPK6, Plays a Role in Leaf Senescence,” Plant Physiol. 150(1):167-177 (2009), which is hereby incorporated by reference in its entirety) to form pGL3135 that allows the overexpression of S3H in plants. (ii) The DNA fragment released from EcoRI-digestion of BAC F7L13 DNA (obtained from ABRC) contained the whole S3H gene including its promoter region and was cloned into the binary vector pPZP211 at EcoRI site to form pGL3228 for the complementation of s3h. (iii) To produce His-tagged S3H recombinant protein, the open reading frame of S3H was PCR amplified using a pair of primers, S3H_BamHI and S3H_HindIII, and was cloned into pET28a (Novagen, Madison, Wis.) to form pET28a-S3H. All constructs were verified by DNA sequencing.
Example 5—Chlorophyll Contents, Fv/Fm Assay and Survival Curves
Chlorophyll was extracted and quantified as described previously (He & Gan, “A Gene Encoding an Acyl Hydrolase Is Involved in Leaf Senescence in Arabidopsis,” Plant Cell 14(4):805-815 (2002), which is hereby incorporated by reference in its entirety). Fluorescence in leaves was measured using a portable chlorophyll fluorometer (model: OS1-FL) according to the manufacturer's instructions (Opti-Sciences, Tyngsboro, Mass.). The ratio of variable fluorescence to maximal fluorescence (Fv/Fm) for each leaf was quantified directly using the fluorometer's test mode 1. The survival curve assay was based on visual observation of leaf yellowing. The completely yellowed leaf was regarded as a dead leaf. The survival rate of WT, s3h and S3HOE1 plants were counted from 20 DAG to 70 DAG.
Example 6—Transcript Analyses
Total RNA extraction and RNA blot analysis were performed as described previously (He & Gan, “A Gene Encoding an Acyl Hydrolase Is Involved in Leaf Senescence in Arabidopsis,” Plant Cell 14(4):805-815 (2002), which is hereby incorporated by reference in its entirety). The DNA templates for probe labeling were amplified by the following different pairs of primers: S3H, G2956 and G2957; AtNAP, G3149 and G3150; SAG12, G10 and G246; SAG13, G9 and G16; EDS1, EDS1F and EDS1R; PAD4, PAD4F and PAD4R; PR1, PR1F and PR1R (FIG. 1E). The ethidium bromide staining of the RNA gels was used to show approximate amount of total RNA loading on each lane.
Example 7—Transformation
The binary vectors were electroporated into the Agrobacterium tumefaciens strain ABI1 and transformed into the WT or s3h mutant via the floral dip method (Clough & Bent, “Floral Dip: A Simplified Method for Agrobacterium-Mediated Transformation of Arabidopsis thaliana,” Plant J. 16(6):735-743 (1998), which is hereby incorporated by reference in its entirety). Approximately 40 kanamycin-resistant T1 transgenic lines for each transgene were selected. Phenotypic analyses were performed in the T2 generation and confirmed in the T3 generation. Homozygous plants were used in all experiments.
Example 8—Metabolite Analysis
All rosette leaves of 25 DAG and 35 DAG plants of WT, s3h and S3HOE1 transgenic plants were collected for analysis of free SA, 2,3-DHBA and 2,5-DHBA using a protocol described previously (Pan et al., “Simultaneous Quantification of Major Phytohormones and Related Compounds in Crude Plant Extracts by Liquid Chromatography-Electrospray Tandem Mass Spectrometry,” Phytochemistry 69(8):1773-1781 (2008), which is hereby incorporated by reference in its entirety). 10 μL extracts were injected for analysis using a liquid chromatography mass-spectrometry (LC-MS) (Quantum Access; Thermo Scientific, Waltham, Mass.) as described (Thaler et al., “Salicylate-Mediated Interactions Between Pathogens and Herbivores,” Ecology 91(4):1075-1082 (2010), which is hereby incorporated by reference in its entirety). The SAG, 2,3-DHBX, 2,3-DHBG, 2,5-DHBX and 2,5-DHBG samples with internal standard [2H4]SA (CDN Isotopes, Pointe-Claire, QC, Canada) were extracted as described previously (Zhang et al., “An Engineered Monolignol 4-o-Methyltransferase Depresses Lignin Biosynthesis and Confers Novel Metabolic Capability in Arabidopsis,” Plant Cell 24(7):3135-3152 (2012), which is hereby incorporated by reference in its entirety). Glycosides were separated on a C18 reversed phase column (Gemini-NX, 3 μm, 150×4.6 mm, Phenomenex, Torrance, Calif.) and the following solvent gradient (solvent A: 0.1% formic acid in water; solvent B: 0.1% formic acid in acetonitrile): initial 5% B to 75% B at 45 min, and 45-52 min 75% B at a flow rate of 0.2 mL min−1. The MS detector was equipped with an electrospray ionization (ESI) probe operated under negative ionization conditions: spray voltage 4.0 kV, capillary temperature 360° C., sheath gas (N2) pressure 15 arbitrary units, auxiliary gas (N2) pressure 10 arbitrary units. Mass spectra were recorded in full scan mode (m/z 100 to m/z 1000) to determine molecular ions [M-H]− used for glycoside quantification. Glycoside identity was verified by comparing UV absorption, retention time, and collision-induced dissociation (CID energy 20 V, CID gas (Ar) pressure 1.5 mTorr) fragmentation of selected molecular ions with published data (Bartsch et al., “Accumulation of Isochorismate-Derived 2,3-Dihydroxybenzoic 3-O-beta-D-Xyloside in Arabidopsis Resistance to Pathogens and Ageing of Leaves,” J. Biol. Chem. 285(33):25654-25665 (2010), which is hereby incorporated by reference in its entirety). Five to six biological replicates of each genotype were analyzed.
Example 9—S3H Recombinant Protein Expression in and Purification from Escherichia coli 
The construct pET28a-S3H was introduced into E. coli BL21 (DE3, pLys3, Invitrogen, Carlsbad, Calif.). The bacterial cells containing pET28a-S3H were induced with 0.5 mM isopropyl β-D-1-thiogalactoside (IPTG) for 24 hr at 18° C., and were collected and disrupted by sonication in lysis buffer (20 mM Tris-Cl, pH7.5, 2 mM EDTA, 2 mM β-mercaptoethanol, 1 mM PMSF, 10 mM Imidazole, 0.1% Triton X-100, 20 mM sodium ascorbate). All subsequent purification procedures involving His-Bind® resin were performed at 4° C. according to the manufacturer (Novagen, Madison, Wis.). The recombinant protein concentration was quantified using Quick Start Bradford Dye Reagent (Biorad, Hercules, Calif.). 2 mM DTT was added to the enzyme solution and the protein was immediately kept in a −80° C. refrigerator.
Example 10—Enzyme Assay
The enzyme assay was performed using a method modified from Satio et al. (Saito et al., “Direct Evidence for Anthocyanidin Synthase as a 2-Oxoglutarate-Dependent Oxygenase: Molecular Cloning and Functional Expression of cDNA From a Red Forma of Perilla frutescens,” Plant J. 17(2):181-189 (1999), which is hereby incorporated by reference in its entirety). The reaction mixture (100 μl) contained 5 mM DTT, 4 mM sodium ascorbate, 1 mM 2-oxoglutaric acid, 0.4 mM FeSO4, 0.1 mg/ml catalase, 50 mM Tris-Cl pH8.0 or other buffer, 8-15 μg recombinant protein, various concentrations of SA. To determine the optimal temperature and pH for enzyme activity of S3H, the protein was incubated with 200 μM SA substrate in Tris-Cl buffer (pH8.0) at different temperatures for 15 min; or 500 μM SA substrate, in 50 mM Sodium citrate, Tris-Cl and Glycine buffers with different pH at 40° C. for 15 min. For enzyme kinetics analysis, various substrate SA concentrations ranging from 10 to 500 μM were used and the incubation proceeded for 6 min with pH 6.0. All the reactions were started by adding the enzyme and stopped by adding 100 μl 50% (v/v) acetonitrile and heated at 99° C. for 1 min to pellet the protein. After centrifugation for 10 min at full speed, the samples were resolved on a Gemini C18 reversephase column (Phenomenex, Torrance, Calif.) in the following solvent gradient (solvent A: 0.2% acetic acid in water; solvent B: 0.2% acetic acid in acetonitrile): initial 5% B to 48% B at 10 min, and 11-15 min 51% B, then 16-21 min 100% B at a flow rate of 1 mL min−1. For MS analysis, an HP 1100 series II LC system (Agilent, Danbury, Conn.) was coupled to a Bruker Esquire ion-trap mass spectrometer (MSD trap XCT system) equipped with an electrospray ionization source. Negative ionization was attained using an ion source voltage of 3.5 kV, a corona of 4000 nA, and a skimmer at a voltage of 40 V. Nebulization was aided by a coaxial nitrogen sheath gas at 50 p.s.i. pressure. Desolvation was insured by a counter current nitrogen flow set at 7 p.s.i. pressure, with both the capillary and vaporizer temperature at 300° C. Mass spectra were recorded over 15 to 800 m/z in the negative mode. Km and Vmax were determined by Graphpad Prism 5 software using non-linear regression for Michaelis-Menten equation.
Sequence described herein can be found in the GenBank/EMBL databases under the following accession numbers, each of which is hereby incorporated by reference in its entirety: AT4G10500 (S3H, SAG108), AT3G51240 (F3H), AT2G14610 (PR1), AT1G69490 (AtNAP), AT5G45890 (SAG12), AT2G29350 (SAG13), AT3G48090 (EDS1), AT3G52430 (PAD4), AT1G74710 (ICS1) and AT1G18870 (ICS2), AT2G43840 (UGT74F1) and AT2G43820 (UGT74F2), AT3G11480 (BSMT1); AT2G23620 (AtMES1), AT2G23600 (AtMES2), AT2G23580 (AtMES4), AT2G23560 (AtMES7), AT4G37150 (AtMES9).
Example 11—Pathogen Test
The pathogen test was essentially followed the method described by Katagiri et al., “The Arabidopsis Thaliana-Pseudomonas Syringae Interaction,” Arabidopsis Book 1:e0039 (2002), which is hereby incorporated by reference in its entirety. The plants used for pathogen test were gown at 20° C. growth chamber with 70-80% relative humidity, under 14 hrs light (120 μmol m−2 sec−1 of fluorescent light). The adult leaves at same position of 4-week plants were used for pathogen inoculation. The bacterial strain Pseudomonas syringae pv tomato DC 3000 was diluted to OD600 0.002 (1×106 cfu/mL) and injected in the middle of a leaf with a needleless syringe. 0 (shortly after inoculation), 1 or 3 days post inoculation, the leaf was washed for 1 min in 70% ethanol and 1 min in water then leaf discs were cut using a cork borer immediately. Subsequently, three leaf discs from three plants were put into 100 μL of distilled water and were immediately homogenized. Appropriate dilutions were plated on Kings B plates and incubated for approximately 2 days at 28° C., and then the colony-forming units for each dilution of each sample were counted.
Example 12—SAG108/S3H is a Senescence-Associated Gene and can be Induced by SA
At4g10500 was initially identified as a senescence-associated gene called SAG108 from previous transcriptomic analyses of leaf senescence in Arabidopsis (Guo et al., “Transcriptome of Arabidopsis Leaf Senescence,” Plant Cell Environ. 27(5):521-549 (2004), which is hereby incorporated by reference in its entirety) and was found to encode a functional S3H as described herein. RNA gel blot analysis revealed that the S3H transcript accumulated in senescing leaves but was barely detectable in non-senescence leaves, which showed similar expression pattern to a known senescence-associated transcription factor AtNAP (FIG. 1A) (Guo & Gan, “AtNAP, a NAC Family Transcription Factor, Has an Important Role in Leaf Senescence,” Plant J. 46(4):601-612 (2006), which is hereby incorporated by reference in its entirety). The S3H expression was also readily inducible after SA treatment for 6 hours, with a similar induction pattern to that of PR1 which is well known for its inducibility by SA (FIG. 1B) (Uknes et al., “Acquired Resistance in Arabidopsis,” Plant Cell 4(6):645-656 (1992), which is hereby incorporated by reference in its entirety).
Example 13—S3H Regulates Onset and Progression of Leaf Senescence
To investigate the biological function of S3H, a T-DNA insertion line (SALK_059907) was characterized. This line contains a T-DNA insertion in the 2nd exon of S3H that completely abolishes S3H expression in homozygous mutant plants as revealed by RNA gel blot analysis (FIGS. 1C and D). The s3h knockout plants exhibited a remarkably accelerated leaf senescence phenotype characterized by lower chlorophyll content and reduced Fv/Fm ratios compared with those of age-matched wild type (WT) (FIGS. 2A-C and FIG. 6B); however, the early stages of growth and development of the plants that were 25 DAG old (days after germination of seeds) appeared to be normal (FIG. 3A and FIG. 6A). The Fv/Fm ratio reflects the activity of photosystem II.
The leaf senescence program in s3h mutants was accelerated in two aspects. First, the senescence rate was accelerated. It took 9.5 days on average for senescence to progress from the leaf tip to the petiole in WT but only 2.7 days in the s3h leaves (FIG. 2G). Second, the onset of senescence was earlier (visible sign of yellowing at the leaf tip); in s3h the onset occurred 16.8 days after emergence (DAE) of leaves, compared to 19.2-DAE in WT (FIG. 2G). The earlier onset of senescence and the faster senescence progression are also observed from the leaf survival curves (FIG. 2H). The s3h null mutation is responsible for the phenotype because a construct (pGL3228) carrying an intact S3H (including its promoter region) restored s3h to WT (FIG. 6C).
In contrast, when S3H was constitutively overexpressed in WT under the direction of the CaMV 35S promoter (FIG. 7), leaf senescence was significantly delayed (FIGS. 2D-F and FIG. 3A), but the flowering time was not altered (FIG. 3A). As shown in FIG. 2D, leaves of the 45-DAG WT plants had been senescing but leaves of the age-matched S3H overexpression transgenic plants remained green. Also, it took 14.6 days for senescence to progress from the leaf tip to the petiole in the S3H overexpression lines (S3HOE) compared to 9.5 and 2.7 days in WT and s3h, respectively (FIG. 2G). The onset of leaf senescence in S3HOE, which occurred at 21.2 DAE, was also delayed compared to 19.2 DAE in WT and 16.8 DAE in s3h, respectively (FIG. 2G).
Example 14—Expression of Senescence-Associated Genes and Defense-Related Genes is Accelerated in s3h and Delayed in S3HOE Lines
In addition to the above phenotypic characterization, RNA gel blot analyses of two widely used leaf senescence marker genes SAG12 and SAG13, and three SA signaling marker genes EDS1, PAD4 and PR1 was performed in leaves of WT, s3h and S3HOE lines at different ages (FIG. 3B). The highly senescence-specific SAG12 transcripts were readily detectable in the rosette leaves of 35-DAG s3h mutant and 40-DAG WT plants, but were not detected in the S3HOE1 plants at 40 DAG. Likewise, the SAG13, PR1, EDS1 and PAD4 were also found to be precociously expressed in the s3h mutants but significantly suppressed in the overexpression lines. These data suggest that S3H regulates senescence-associated genes and genes involved in SA signaling.
Example 15—S3H Mediates the Conversion of SA to 2,3-DHBA in Planta
The metabolite profiles of SA in young and senescing plants of WT, s3h and S3HOE lines were analyzed using LC-MS/MS. The levels of free SA, SA sugar conjugates, SA derivatives including 2,3-DHBA, 2,5-DHBA and their sugar conjugates are summarized in FIG. 4H.
The free SA levels in rosette leaves of young (Y) or senescing (S) s3h mutant plants were 625% and 710% of those of WT, whereas the free SA concentrations in rosette leaves of young or senescing S3HOE1 lines were reduced to only 10-12.5% of those in WT. Similarly, the concentrations of SA glucosides (SAG) in leaves of young or senescing s3h mutant plants were also increased to 267% and 317% of those of WT, respectively. However, the SAG levels were reduced to undetectable levels in leaves of both young and senescing S3HOE1 lines (FIG. 4H).
In contrast to SA, the levels of free 2,3-DHBA and 2,5-DHBA, two major catabolites of SA in Arabidopsis, were too low to be detected but the levels of their sugar conjugates were drastically changed. In s3h mutant, free SA and SAG were accumulated to very high levels as mentioned above. However, the total levels of 2,3-DHBA sugar conjugates (2,3-DHBX+2,3-DHBG) were not detected in the s3h mutant while the total levels of 2,5-DHBA sugar conjugates (2,5-DHBX+2,5-DHBG) were increased to 113% and 162% of those of WT in the young leaves and senescing leaves, respectively. By contrast, in the S3HOE1 leaves, the levels of both 2,3-DHBA sugar conjugates (2,3-DHBX+2,3-DHBG) were significantly increased to 187% and 224% of those of rosette leaves of WT (Y or S), respectively, but the levels of 2,5-DHBA sugar conjugates (2,5-DHBX+2,5-DHBG) were significantly reduced to 43% and 40% of those of WT, respectively (FIG. 4H). These data strongly suggest that S3H converts SA to 2,3-DHBA in vivo.
Example 16—Recombinant S3H Possesses SA 3- and 5-Hydroxylase Activities
Sequence analyses revealed that S3H encodes a protein with similarity to the 2OG-Fe(II) oxygenase family enzymes (including a well-known F3H enzyme) that have a conserved catalytic domain Pfam PF03171 (FIGS. 8 and 9) (Wisman et al., “Knock-out Mutants From an En-1 Mutagenized Arabidopsis thaliana Population Generate Phenylpropanoid Biosynthesis Phenotypes,” Proc. Nat'l Acad. Sci. U.S.A. 95(21):12432-12437 (1998), which is hereby incorporated by reference in its entirety). Because the above phytochemical analyses implied that S3H catalyzes SA to produce 2,3-DHBA, biochemical analyses was performed to confirm the enzymatic activity using recombinant S3H enzyme overproduced in and purified from E. coli. To eliminate non-enzymatic oxidation of SA due to hydroxyl radicals (.OH), catalase was added to each reaction to remove the hydroxyl radicals. The recombinant S3H enzyme converted SA to both 2,3-DHBA and 2,5-DHBA in the presence of ferrous iron, ascorbate, 2-oxoglutarate (20G) and catalase (FIGS. 4A and B). The 2,3-DHBA and 2,5-DHBA produced by the recombinant S3H have the same retention times and UV spectra as those of the 2,3-DHBA and 2,5-DHBA authentic standards, respectively (FIG. 4B-D). The mass spectra of produced 2,3-DHBA and 2,5-DHBA were also characterized by LC-MS/MS (FIG. 4E-F). The enzyme activity increased with the increase of temperature from 4° C. to 40° C. and decreased from 40° C. to 50° C., suggesting the optimal temperature of this enzyme is approximate 40° C. (FIG. 10A). The effect of pH on the enzyme activities was also tested and the optimal pH was 6.0 under the test conditions (FIG. 10B). The apparent Km value for SA of the recombinant S3H was determined to be 58.29 μM at the optimal temperature and pH conditions (FIG. 4G). The substrate specificity of the recombinant S3H was also investigated using benzoic acid and anthranilic acid, two chemicals with similar structures to SA. The S3H enzyme did not have detectable activity after 30-min reaction under the same conditions as those for SA (Table 1), suggesting that the recombinant S3H enzyme has high substrate specificity.







TABLE 1







Substrate Specificity of S3Ha












Specific






activity

Vmax




(nmol

(nmol
Kcat/Km


Substrates
mg−1 min−1)
Km (μM)
mg−1 min−1)
(M−1s−1)





Salicylic acid
78.06 ± 9.75
58.29 ± 5.27
497.8 ± 12.77
6.09 × 103


Benzoic acid
n.d.b


Anthranilic
n.d


acid





aThe specific activity was measured from the reaction at pH 6.0 and 40° C. for 30 min, the data represent the mean ± SE of three replicates. Kinetic parameters were obtained from the reactions at pH 6.0 and 40° C. for 6 min as described in methods, the data represent the mean ± SE of four replicates


bn.d. not detectable.






The data described herein has revealed a novel negative feedback regulation mechanism by which plants modulate their endogenous SA levels at the onset of and during leaf senescence and has provided strong molecular genetic evidence that S3H, via modulating SA levels, has a pivotal role in controlling leaf senescence.
Elucidation of biosynthesis and catabolism of SA is important for understanding its biological functions. The PAL and IC pathway have been well studied and proposed to be the two main routes responsible for SA biosynthesis (Dempsey et al., “Salicylic Acid Biosynthesis and Metabolism,” Arabidopsis Book 9:e0156 (2011), which is hereby incorporated by reference in its entirety). However, the catabolism of SA is less understood (Dempsey et al., “Salicylic Acid Biosynthesis and Metabolism,” Arabidopsis Book 9:e0156 (2011), which is hereby incorporated by reference in its entirety). This identification and characterization of S3H uncovered a negative feedback regulatory mechanism by which plants regulate SA levels. Briefly, S3H is induced by SA and the induced S3H enzyme in turn hydrolyzes SA to 2,3-DHBA, a de-activated form of SA (Bartsch et al., “Accumulation of Isochorismate-Derived 2,3-Dihydroxybenzoic 3-O-beta-D-Xyloside in Arabidopsis Resistance to Pathogens and Ageing of Leaves,” J. Biol. Chem. 285(33):25654-25665 (2010) and Hennig et al., “Interconversion of the Salicylic Acid Signal and its Glucoside in Tobacco,” Plant J. 4(4):593-600 (1993), each of which is hereby incorporated by reference in its entirety), to prevent over-accumulation of SA.
The feedback regulation is supported by the facts that (i) S3H is readily induced by the treatment with SA (FIG. 1B and Table 2), and (ii) S3H is highly expressed in senescing leaves (FIG. 1A), which is most likely caused by the elevated levels of endogenous SA in senescing leaves (FIG. 4H; Bartsch et al., “Accumulation of Isochorismate-Derived 2,3-Dihydroxybenzoic 3-O-beta-D-Xyloside in Arabidopsis Resistance to Pathogens and Ageing of Leaves,” J. Biol. Chem. 285(33):25654-25665 (2010) and Morris et al., “Salicylic Acid has a Role in Regulating Gene Expression During Leaf Senescence,” Plant J. 23(5):677-685 (2000), each of which is hereby incorporated by reference in its entirety)). In senescing leaves of the SA-deficient NahG transgenic plants, the expression of S3H is almost undetectable but in the senescing leaves of the JA-deficient mutant coi1 and the ethylene-signaling mutant ein2 (as controls), S3H expression is not affected (FIG. 11) (Buchanan-Wollaston et al., “Comparative Transcriptome Analysis Reveals Significant Differences in Gene Expression and Signalling Pathways Between Developmental and Dark/Starvation-Induced Senescence in Arabidopsis,” Plant J. 42(4):567-585 (2005), which is hereby incorporated by reference in its entirety).







TABLE 2







S3H was induced by SA and pathogensa














Treat
Induction


Stimulus
Concentration
Materials
time
folds















Salicylic acid
10
μM
Col-0 (Seedling)
 3 hrs
36.72


Pstb(DC3000)
2 × 105
cfu/ml
Ler (Adult leaf)
12 hrs
11.73


Pst (DC3000)
1 × 105
cfu/ml
Col-0 (Adult leaf)
48 hrs
4.7


Pst (DC3000, AvrRpt1)
1 × 108
cfu/ml
Col-0 (Adult leaf)
 6 hrs
4.41


Pst (DC3000, AvrRpt2)
1 × 105
cfu/ml
Col-0 (Adult leaf)
48 hrs
5.09


Pst (DC3118)
2 × 107
cfu/ml
Col-5 (Adult leaf)
10 hrs
8.54


H. arabidopsis
5 × 105
spores/ml
Col-0 (Adult leaf)
 4 days
10.61


H. arabidopsis
5 × 105
spores/ml
Col-0 (Seedling)
 6 days
4.89











Turnip mosaic virus
n.dc.
Col-0 (Adult leaf)
Zoned 0
26.24





aData were collected from perturbation treatments in (genevestigator.com).


bPst, P.syringe pv. Tomato.


cNo description.


dZone represents viral infection stage.






The feedback regulation is further supported by the demonstration of S3H's enzymatic activity. Although the recombinant S3H possesses both 3-hydroxylase and 5-hydroxylase activities that convert SA to respective 2,3- and 2,5-DHBA in vitro (FIG. 4), S3H most likely acts as SA 3-hydroxylase in vivo because disruption of S3H renders 2,3-DHBA sugar conjugates undetectable whereas overexpression of S3H leads to a very high level accumulation of the 2,3-DHBA sugar conjugates (FIG. 4H). In contrast, the levels of 2,5-DHBA sugar conjugates increased in s3h and decreased in the overexpression lines, respectively (FIG. 4H), presumably resulting from changes in the SA metabolic flux in these mutants. For example, as shown in FIG. 5, because the conversion of SA to 2,3-DHBA is blocked in s3h, more SA becomes available for its conversion to 2,5-DHBA. It has been postulated that 2,3- and 2,5-DHBA species are products of SA oxidation by reactive oxygen species (ROS) (Bartsch et al., “Accumulation of Isochorismate-Derived 2,3-Dihydroxybenzoic 3-O-beta-D-Xyloside in Arabidopsis Resistance to Pathogens and Ageing of Leaves,” J. Biol. Chem. 285(33):25654-25665 (2010), which is hereby incorporated by reference in its entirety). This research, however, provides direct evidence that 2,3-DHBA is enzymatically synthesized from SA, which enriches current understanding of SA metabolic pathways as shown in FIG. 5. Whether 2,5-DHBA is also enzymatically formed needs to be investigated.
The experimental results reported herein also provide strong molecular genetic evidence that S3H has a pivotal role in regulating the onset and rate of leaf senescence in Arabidopsis by modulating the endogenous levels of SA. S3H was initially identified during leaf senescence transcriptomic analyses (Guo et al., “Transcriptome of Arabidopsis Leaf Senescence,” Plant Cell Environ. 27(5):521-549 (2004), each of which is hereby incorporated by reference in its entirety) as a senescence-associated gene whose transcript levels increase with the progression of leaf senescence (FIG. 1A). Leaf senescence was significantly accelerated in the T-DNA insertion s3h knockout plants but remarkably attenuated in the S3H overexpression lines (FIGS. 2 and 3A). Specifically, under the experimental growth conditions, a WT leaf started to senesce (as visible sign of yellowing at the leaf tip) at 19.2 DAE and took 9.5 days for the senescence to progress from the tip to the petiole. By comparison, a leaf of s3h or S3HOE began senescence 2.4 days earlier or 2 days later than that of WT, respectively. The rate of leaf senescence was also 72% faster in s3h and 54% slower in S3HOE than the rate of WT (FIG. 2G). Consistently, the expression of the leaf senescence marker genes SAG12 and SAG13 was also enhanced in s3h and inhibited in S3HOE (FIG. 3B).
S3H regulates leaf senescence most likely by controlling the SA levels. As discussed above, S3H modulates the SA levels in plants via a negative feedback regulatory mechanism. The SA levels in the senescing leaves were higher than those in the young leaves (FIG. 4H). Furthermore, the SA levels in s3h were much higher than in WT (FIG. 4H). In contrast, the SA levels in S3HOE were much lower (FIG. 4H). Previous correlative studies that revealed low SA levels in young leaves and high concentration in senescing leaves have suggested that SA promotes leaf senescence (Gan, in Plant Hormones: Biosynthesis, Signal Transduction, Action!, Ed. Davies (Springer, Dordrecht), pp 597-617 (2010), which is hereby incorporated by reference in its entirety). The suppression of SAG101, whose product interacts with PAD4 and EDS1 of SA signaling (Zhu et al., “SAG101 Forms a Ternary Complex With EDS1 and PAD4 and Is Required for Resistance Signaling Against Turnip Crinkle Virus,” PLoS Pathog. 7(11):e1002318 (2011), which is hereby incorporated by reference in its entirety), displayed a delay in leaf senescence (He & Gan, “A Gene Encoding an Acyl Hydrolase Is Involved in Leaf Senescence in Arabidopsis,” Plant Cell 14(4):805-815 (2002), which is hereby incorporated by reference in its entirety), also supporting a role of SA in leaf senescence. Overexpression of a bacterial gene named NahG reduced the SA levels and delayed leaf senescence, which further supports a role of SA in promoting leaf senescence (Morris et al., “Salicylic Acid has a Role in Regulating Gene Expression During Leaf Senescence,” Plant J. 23(5):677-685 (2000), which is hereby incorporated by reference in its entirety). This research provides two lines of evidence for SA's role in leaf senescence. One line of evidence comes from S3HOE plants that, like NahG plants, have very low levels of SA (FIG. 4H) and display a significant delay in leaf senescence (FIGS. 2D and 3A). The other line of the evidence comes from s3h knockout plants that accumulate high levels of SA (FIG. 4H) and exhibit an early senescence phenotype (FIGS. 2A and 3A). It should be noted that although Nah G-overexpressing plants have been useful in many studies involving SA, the introduction of the bacterial gene may complicate interpretation of the data. For example, the NahG enzyme converts SA to catechol, which is quite different from the natural metabolites of SA in plants. Therefore, the S3HOE may be a better system for SA-related research in the future.
In addition to leaf senescence, S3H appears to have a role in plant defense. Analyses of published (Wang et al., “Timing of Plant Immune Responses by a Central Circadian Regulator,” Nature 470(7332):110-114 (2011), which is hereby incorporated by reference in its entirety) and other online microarray data (genevestigator.com) revealed that S3H can be induced by pathogen infection (FIGS. 12A-12C and Table 2). Consistently, the levels of 2,3-DHBA (the product of S3H-catalyzed reaction) derivatives were increased in Arabidopsis after challenging with pathogen (Bartsch et al., “Accumulation of Isochorismate-Derived 2,3-Dihydroxybenzoic 3-O-beta-D-Xyloside in Arabidopsis Resistance to Pathogens and Ageing of Leaves,” J. Biol. Chem. 285(33):25654-25665 (2010), which is hereby incorporated by reference in its entirety). Knockout and overexpression of this gene resulted in enhanced and reduced resistance to Pseudomonas syringae pv. Tomato DC3000, respectively (FIG. 13).
In addition FIG. 8 is a phylogeny of S3H and its homologues in Arabidopsis genome. The deduced protein of S3H was used to search the protein sequence of its homologues and the phylogenetic tree was generated with DNAMAN software (version 7).
Although preferred embodiments have been depicted and described in detail herein, it will be apparent to those skilled in the relevant art that various modifications, additions, substitutions, and the like can be made without departing from the spirit of the invention and these are therefore considered to be within the scope of the invention as defined in the claims which follow.

## Claims

1. A transgenic plant having a reduced level of salicylic acid 3-hydroxylase (“S3H”), compared to that of a non-transgenic plant, wherein the transgenic plant displays a precocious or premature leaf senescence phenotype, relative to a non-transgenic plant, wherein the plant is transformed with a nucleic acid construct comprising a nucleic acid molecule configured to silence expression of an S3H protein having the amino acid sequence of SEQ ID NO: 3.

2. The plant according to claim 1, wherein the plant is selected from the group consisting of rice, corn, soybean, canola, potato, wheat, mung bean, alfalfa, barley, rye, cotton, sunflower, peanut, sweet potato, bean, pea, chicory, lettuce, endive, cabbage, brussel sprout, beet, parsnip, turnip, cauliflower, broccoli, radish, spinach, onion, garlic, eggplant, pepper, celery, carrot, squash, pumpkin, zucchini, cucumber, apple, pear, melon, citrus, strawberry, grape, raspberry, pineapple, tobacco, tomato, sorghum, sugarcane, banana, Arabidopsis thaliana, Saintpaulia, petunia, pelargonium, poinsettia, chrysanthemum, carnation, crocus, marigold, daffodil, pine, Medicago truncatula, Sandersonia aurantiaca, Populus trichocarpa, and zinnia.

3. Seed produced from the plant according to claim 1, wherein the seed comprises the nucleic acid construct.

4. A transgenic plant having an increased level of S3H, compared to that of a non-transgenic plant, wherein said transgenic plant displays a delayed leaf senescence phenotype, relative to a non-transgenic plant, wherein the plant is transformed with a nucleic acid construct that encodes an S3H protein having the amino acid sequence of SEQ ID NO: 3.

5. The plant according to claim 4, wherein the plant is selected from the group consisting of rice, corn, soybean, canola, potato, wheat, mung bean, alfalfa, barley, rye, cotton, sunflower, peanut, sweet potato, bean, pea, chicory, lettuce, endive, cabbage, brussel sprout, beet, parsnip, turnip, cauliflower, broccoli, radish, spinach, onion, garlic, eggplant, pepper, celery, carrot, squash, pumpkin, zucchini, cucumber, apple, pear, melon, citrus, strawberry, grape, raspberry, pineapple, tobacco, tomato, sorghum, sugarcane, banana, Arabidopsis thaliana, Saintpaulia, petunia, pelargonium, poinsettia, chrysanthemum, carnation, crocus, marigold, daffodil, pine, Medicago truncatula, Sandersonia aurantiaca, Populus trichocarpa, and zinnia.

6. Seed produced from the plant according to claim 4, wherein the seed comprises the nucleic acid construct.

7. A mutant plant comprising an inactivated gene encoding an S3H protein having the amino acid sequence of SEQ ID NO:3, wherein the mutant plant displays a premature or precocious leaf senescence phenotype, relative to a non-mutant plant.

8. A method for promoting premature or precocious leaf senescence in a plant, said method comprising:
providing a transgenic plant or plant seed transformed with a nucleic acid construct effective in silencing expression of an S3H protein having the amino acid sequence of SEQ ID NO:3 and
growing the transgenic plant or the plant grown from the transgenic plant seed under conditions effective to promote premature or precocious leaf senescence in the transgenic plant or the plant grown from the transgenic plant seed.

9. The method according to claim 8, wherein said nucleic acid construct comprises:
a nucleic acid molecule configured to silence the S3H protein expression;
a 5′ DNA promoter sequence; and
a 3′ terminator sequence, wherein the nucleic acid molecule, the promoter, and the terminator are operatively coupled to permit expression of the nucleic acid molecule.

10. A method for delaying leaf senescence in a plant, said method comprising:
transforming a plant cell with a nucleic acid molecule encoding an S3H protein comprising the amino acid sequence of SEQ ID NO: 3, wherein the nucleic acid molecule is operably associated with a promoter to obtain a transformed plant cell, wherein expression of the nucleic acid molecule in the plant cell causes delayed leaf senescence; and
regenerating a plant from the transformed plant cell under conditions effective to delay leaf senescence in the plant.

11. The method according to claim 10, wherein said transforming comprises transforming the plant cell with a nucleic acid construct comprising:
the nucleic acid molecule;
a 5′ heterologous DNA promoter sequence; and
a 3′ terminator sequence, wherein the nucleic acid molecule, the DNA promoter sequence, and the terminator sequence are operatively coupled to permit transcription of the nucleic acid molecule.

12. A method of making a mutant plant having a decreased level of S3H protein compared to that of a non-mutant plant, wherein the mutant plant displays a premature or precocious leaf senescence phenotype relative to a non-mutant plant, said method comprising:
providing at least one cell of a non-mutant plant containing a gene encoding a functional S3H protein comprising the amino acid sequence of SEQ ID NO:3;
treating said at least one cell of a non-mutant plant under conditions effective to inactivate said gene, thereby yielding at least one mutant plant cell containing an inactivated S3H protein encoding gene; and
propagating said at least one mutant plant cell into a mutant plant, wherein said mutant plant has a decreased level of S3H protein compared to that of the non-mutant plant and displays a premature or precocious leaf senescence phenotype relative to a non-mutant plant.

13. A method of inducing or promoting pathogen resistance in plants, said method comprising:
providing a transgenic plant or plant seed transformed with a nucleic acid construct effective in silencing expression of an S3H protein comprising the amino acid sequence of SEQ ID NO:3; and
growing the transgenic plant or the plant grown from the transgenic plant seed under conditions effective to induce or promote pathogen resistance in the transgenic plant or the plant grown from the transgenic plant seed.