Title

Application for an Experimental Use Permit for 1507x59122xMON810, 

1507xMON810 and 59122xMON810 

Data Requirement

40 CFR Part 172 

Authors

Rachel Binning, Keri Henderson, Dave Langer, Scott Mundell, Tracy Rood and Jamie Staley 

Original Submission Date

August 14, 2008 

Submission of Updated Version

April 20, 2009 

Submitter

Tracy A. Rood 

Pioneer Hi-Bred International, Inc. 

7250 NW 62nd Ave. 

Johnston, IA 50131 

Submitter Reference Number

PHI-2008-139 

Volume 1 
PHI-2008-139 

April 20, 2009Page 2 of 416
Volume 1

Table of Contents 

Page

Statement of Data Confidentiality Claim 2 

Transmittal Document 5 

EPA Form 8570-17 (Application for Experimental Use Permit) 7 

EPA Form 8570-4 (Confidential Statements of Formula) 8 

EPA Form 8570-34 (Certification with Respect to Citation of Data) 9 

EPA Form 8570-35 (Data Matrix for 1507x59122xMON810) 10 

EPA Form 8570-35 (Data Matrix for 1507xMON810) 65 

EPA Form 8570-35 (Data Matrix for 59122xMON810) 98 

Section A. Product Chemistry 131 

Section B. Proposed Labels 132 

Section C. Reference to Available Environmental Toxicity Data 193 

Section D. Residue Data 194 

Section E. Effectiveness Data 195 

Section F. Tolerance if Testing Involves Use in Food or Feed 199 

Section G. Proposed Experimental Program 200 

G1. Participants in the Experimental Program 200 

G2. Acreage and Seed Quantities by State or Territory for the EUP Program 203 

G3. Details of the Proposed EUP Program 213 

G4. Definitions 213 

G5. Universal Protocol Procedures 214 

G6. Special Protocol Exemptions 215 

Nursery / Breeding Protocol 219 

Yield / Agronomic Protocol 221 

Efficacy / IRM Protocol 223 

Efficacy / IRM Protocol with No Isolation 225 

Seed Production Protocol 226 

Regulatory Protocol 228 

Confidential Attachment 230 

PHI-2008-139 

April 20, 2009Page 3 of 416
List of Tables and Figures 

Tables 

Page

Table 1 Cross-reference Event Table 131 

Table 2. Lepidopteran Pests That Are Controlled or Suppressed by Pyramided 

1507xMON810 195 

Table 3. Efficacy of Breeding Stacks Against First Generation European Corn Borer 197 

Table 4. Efficacy of Breeding Stacks Against Fall Armyworm 198 

Table 5. List of Participants 200 

Table 6. Breeding Stacks Included in Categories 1, 2 and 3 203 

Table 7. Proposed Total Acreage by State 205 

Table 8. Estimated Acreage per State/Territory for Nursery / Breeding Protocol 206 

Table 9. Estimated Acreage per State/Territory for Yield / Agronomic Protocol 207 

Table 10. Estimated Acreage per State/Territory for Efficacy / IRM Protocol 208 

Table 11. Estimated Acreage per State/Territory for Efficacy / IRM Protocol with No Isolation 209 

Table 12. Estimated Acreage per State/Territory for Seed Production Protocol 210 

Table 13. Estimated Acreage per State/Territory for Regulatory Protocol 211 

Table 14. Summary of EUP Program Quantities for 2009 212 

Figures 

Figure 1. Example Regulated Transgenic Site Map 216 

Figure 2. Example Trial Site Map with No Isolation 217 

Figure 3. Example of Master Label Weight Summary 218 

PHI-2008-139 

April 20, 2009Page 4 of 416
Transmittal Document

Submitter

Pioneer Hi-Bred International, Inc. 

7250 NW 62nd Ave. 

Johnston, IA 50131 

Regulatory Action in Support of Which This Document is Submitted

Application for an Experimental Use Permit with a Plant-Incorporated Protectant 

Pursuant to FIFRA §5(a): 

Application for an Experimental Use Permit for 1507x59122xMON810, 

1507xMON810 and 59122XMON810 

29964-EUPOriginal Transmittal Date

August 14, 2008 

Submission of Updated Version

April 20, 2009 

Submitter Reference Number

PHI-2008-139 

PHI-2008-139 

April 20, 2009Page 5 of 416
PHI-2008-139 

April 20, 2009Page 6 of 416
PHI-2008-139 

April 20, 2009Page 7 of 416
PHI-2008-139 Page 8 of 237 

August 14, 2008 

EPA Forms 8570-4

Confidential Statement of Formula for 1507x59122xMON810 

Confidential Statement of Formula for 1507xMON810 

Confidential Statement of Formula for 59122xMON810 

{See Confidential Appendix} 

PHI-2008-139 

April 20, 2009Page 8 of 416
PHI-2008-139 

April 20, 2009Page 9 of 416
PHI-2008-139 Page 10 of 230 

August 14, 2008 

 

EPA Form 8570-35

Data Matrix for 1507x59122xMON810 (see following pages) 

PHI-2008-139 

April 20, 2009Page 10 of 416






















































PHI-2008-139 

 Page 65 of 237 

August 14, 2008 

 

EPA Form 8570-35

Data Matrix for 1507xMON810 (see following pages) 

PHI-2008-139 

April 20, 2009Page 65 of 416
































PHI-2008-139 Page 98 of 237 

August 14, 2008 

 

EPA Form 8570-35

Data Matrix for 59122xMON810 (see following pages) 

PHI-2008-139 

April 20, 2009Page 98 of 416
































PHI-2008-139 Page 131 of 237 

August 14, 2008 

 

Sections A Through G of the EUP Application

Section A. Product Chemistry 

Pioneer is developing two new products that pyramid Lepidopteran insecticidal corn traits. These 

products are being made by crossing registered events using conventional breeding. The 1507xMON180 

product expresses the Cry1F and Cry1Ab Bt-derived protein, each highly selective and effective in 

controlling certain economically important lepidopteran insect pests of field corn. For markets where 

control of corn rootworm is necessary, Pioneer is developing the 1507x59122xMON810 product by 

adding (also by conventional breeding) a third registered event that expresses the Cry34/35Ab1 protein, 

active against corn rootworm larvae. The 59122xMON810 breeding stack is not intended as a 

commercial product, but rather will be used as a breeding intermediate and/or inbred for product 

development of the 1507x59122xMON810 breeding stack. 

Combining Cry1F and Cry1Ab brings together two distinct modes of action against key lepidopteran 

pests. Research has shown that Cry1F and Cry1Ab do not share any high-affinity binding sites (Perreia 

thesis). Pyramiding the 1507 and MON810 events will reduce the risk of resistance in insects controlled 

by both events. 

Enclosed is an application for an Experimental Use Permit (EUP) for field testing of insect-resistant corn. 

The application is for new (non-registered) combinations of previously registered events: DAS-Ø15Ø7-1 

(also known as 1507), DAS-59122-7 (also known as 59122), and MON-ØØ81Ø-6 (also known as 

MON810). These combinations are as follows: 

1) DAS-Ø15Ø7-1xDAS-59122-7xMON-ØØ81Ø-6 (1507x59122xMON810) 

2) DAS-Ø15Ø7-1xMON-ØØ81Ø-6 (1507xMON810) 

3) DAS-59122-7xMON-ØØ81Ø-6 (59122xMON810) 

1507 corn expresses the Cry1F protein that is insecticidally active against certain lepidopteran pests of 

corn. MON810 expresses the Cry1Ab protein that is likewise active against certain lepidopteran pests of 

corn. In a pyramided breeding combination, these two events express two Cry proteins for enhanced 

pest spectrum and insect resistance management/durability. Also included in the EUP is a triple breeding 

stack that includes the 59122 event, which expresses the Cry34/35Ab1 protein for control of corn 

rootworm pests. The 59122xMON810 breeding stack is not intended as a commercial product, but rather 

will be used as a breeding intermediate and/or inbred for product development of the 

1507x59122xMON810 breeding stack. 

Table 1 specifies the registered events that comprise the combinations that are the subject of the EUP: 

Table 1. Cross-reference Event Table 

OECD Event 

Name 

Event Name 

Abbreviation Product Name Registration Number PIP Expressed 

DAS-Ø15Ø7-1 1507 Herculex1 I 29964-3 Cry1F 

DAS-59122-7 59122 Herculex 

Rootworm 29964-4 Cry34/35 

MON-ØØ81Ø-6 MON810 YieldGard2

 Corn 

Borer 524-489 Cry1Ab 

 

1

 Herculex® I Insect Protection technology by Dow AgroSciences and Pioneer Hi-Bred. Herculex is a registered 

trademark of Dow AgroSciences LLC. 

4

 YieldGard® is a registered trademark used under license from Monsanto Company. 

PHI-2008-139 

April 20, 2009Page 131 of 416
PHI-2008-139 Page 132 of 237 

August 14, 2008 

 

Section B. Proposed Labels 

The following pages contain the proposed Master Label for the EUP, including labels for the EUP Test 

Plants (1-3), as well as labels for registered PIPs (4-13) and non-registered PIPs (14-34) that will be 

planted in EUP experiments. 

PHI-2008-139 

April 20, 2009Page 132 of 416
Master Label 

29964-EUP-6 

Subparts: 

1) 1507 x 59122 x MON810 Corn Seed 

2) 1507 x MON810 Corn Seed 

3) 59122 x MON810 Corn Seed 

4) 1507 Corn Seed 

5) 59122 Corn Seed 

6) MON810 Corn Seed 

7) 1507 x 59122 Corn Seed 

8) MIR604 Corn Seed 

9) Bt11 Corn Seed 

10) Bt11 x MIR604 Corn Seed 

11) MIR162 

12) Bt11 x MIR162 

13) Bt11 x MIR162 x MIR604 

14) 1507 x 59122 x MON810 x MIR162 

15) 1507 x 59122 x MON810 x MIR604 

16) 1507 x 59122 x MIR162 x MIR604 

17) 1507 x MON810 x MIR162 x MIR604 

18) 59122 x MON810 x MIR162 x MIR604 

19) 1507 x 59122 x MIR162 

20) 1507 x 59122 x MIR604 

21) 1507 x MON810 x MIR162 

22) 1507 x MON810 x MIR604 

23) 1507 x MIR162 x MIR604 

24) 59122 x MON810 x MIR162 

25) 59122 x MON810 x MIR604 

26) 59122 x MIR162 x MIR604 

27) MON810 x MIR162 x MIR604 

28) 1507 x MIR162 

29) 1507 x MIR604 

30) 59122 x MIR162 

31) 59122 x MIR604 

32) MON810 x MIR162 

33) MON810 x MIR604 

34) MIR162 x MIR604 

PHI-2008-139 

April 20, 2009Page 133 of 416
1) 1507 x 59122 x MON810 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with 

activity against certain lepidopteran and corn rootworm pests: Cry1F, Cry34/35Ab1, and Cry1Ab. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the 

terms and conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the 

user at the time the corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry1F protein in DAS-Ø15Ø7-1 

and the genetic material necessary for its production in corn................................................................<0.00036%* 

Bacillus thuringiensis Cry34Ab1 protein in DAS-59122-7 

and the genetic material necessary for its production in corn..................................................................<0.0036%* 

Bacillus thuringiensis Cry35Ab1 protein in DAS-59122-7 

and the genetic material necessary for its production in corn................................................................<0.00023%* 

Bacillus thuringiensis Cry1Ab protein in MON-ØØ81Ø-6 

and the genetic material necessary for its production in corn.............................................................<0.000033%* 

Inert Ingredient:

A marker protein and the genetic material 

necessary for its production in event DAS-Ø15Ø7-1 & DAS-59122-7.................................................<0.000016%* 

* Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Pioneer Hi-Bred International, Inc. 

 7300 NW 62nd Ave. 

 Johnston, IA 50131 

NET CONTENTS: ___________pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed 

may only be used according to the field protocols as specified in the approved EUP program. Participants 

and Cooperators must have a copy of each applicable protocol prior to initiating any research with these 

seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened 

lepidopteran and coleopteran species as listed by the US Fish and Wildlife Service. 

PHI-2008-139 

April 20, 2009Page 134 of 416
STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any 

seed not used in these experiments must be returned to the seed provider or disposed of as specified in the 

field protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds 

before destroying. 

PHI-2008-139 

April 20, 2009Page 135 of 416
2) 1507 x MON810 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with 

activity against certain lepidopteran pests: Cry1F and Cry1Ab. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the 

terms and conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the 

user at the time the corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry1F protein in DAS-Ø15Ø7-1 

and the genetic material necessary for its production in corn..................................................................<0.0003%* 

Bacillus thuringiensis Cry1Ab protein in MON-ØØ81Ø-6 

and the genetic material necessary for its production in corn..............................................................<0.000036%* 

Inert Ingredient:

A marker protein and the genetic material 

necessary for its production in event DAS-Ø15Ø7-1.........................................................................<0.0000069%* 

* Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Pioneer Hi-Bred International, Inc. 

 7300 NW 62nd Ave. 

 Johnston, IA 50131 

NET CONTENTS: _______________ pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed 

may only be used according to the field protocols as specified in the approved EUP program. Participants 

and Cooperators must have a copy of each applicable protocol prior to initiating any research with these 

seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened 

lepidopteran species as listed by the US Fish and Wildlife Service. 

PHI-2008-139 

April 20, 2009Page 136 of 416
STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any 

seed not used in these experiments must be returned to the seed provider or disposed of as specified in the 

field protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds 

before destroying. 

PHI-2008-139 

April 20, 2009Page 137 of 416
3) 59122 x MON810 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with 

activity against certain lepidopteran and corn rootworm pests: Cry34/35Ab1 and Cry1Ab. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the 

terms and conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the 

user at the time the corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry34Ab1 protein in DAS-59122-7 

and the genetic material necessary for its production in corn..................................................................<0.0045%* 

Bacillus thuringiensis Cry35Ab1 protein in DAS-59122-7 

and the genetic material necessary for its production in corn................................................................<0.00023%* 

Bacillus thuringiensis Cry1Ab protein in MON-ØØ81Ø-6 

and the genetic material necessary for its production in corn.............................................................<0.000039%* 

Inert Ingredient:

A marker protein and the genetic material 

necessary for its production in event DAS-59122-7...........................................................................<0.0000069%* 

* Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Pioneer Hi-Bred International, Inc. 

 7300 NW 62nd Ave. 

 Johnston, IA 50131 

NET CONTENTS: _______________ pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed 

may only be used according to the field protocols as specified in the approved EUP program. Participants 

and Cooperators must have a copy of each applicable protocol prior to initiating any research with these 

seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened 

lepidopteran and coleopteran species as listed by the US Fish and Wildlife Service. 

PHI-2008-139 

April 20, 2009Page 138 of 416
STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any 

seed not used in these experiments must be returned to the seed provider or disposed of as specified in the 

field protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds 

before destroying. 

PHI-2008-139 

April 20, 2009Page 139 of 416
4) 1507 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with 

activity against certain lepidopteran pests: Cry1F. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the 

terms and conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the 

user at the time the corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry1F protein in DAS-Ø15Ø7-1 

and the genetic material necessary for its production in corn................................................................<0.01415%* 

Inert Ingredient:

A marker protein and the genetic material 

necessary for its production in event DAS-Ø15Ø7-1...............................................................................<0.0020%* 

* Maximum percent (wt/wt) of total extractable protein in grain 

CAUTION 

Keep Out of Reach of Children 

EPA REG NO.: 29964-3 

EPA Establishment Number: 29964-IA-001 Pioneer Hi-Bred International, Inc. 

 7300 NW 62nd Ave. 

 Johnston, IA 50131 

NET CONTENTS: _______________ pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed 

may only be used according to the field protocols as specified in the approved EUP program. Participants 

and Cooperators must have a copy of each applicable protocol prior to initiating any research with these 

seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened 

lepidopteran species as listed by the US Fish and Wildlife Service. 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any 

seed not used in these experiments must be returned to the seed provider or disposed of as specified in the 

field protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds 

before destroying. 

PHI-2008-139 

April 20, 2009Page 140 of 416
5) 59122 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with 

activity against certain corn rootworm pests: Cry34/35Ab1. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the 

terms and conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the 

user at the time the corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry34Ab1 protein in DAS-59122-7 

and the genetic material necessary for its production in corn..................................................................<0.0117%* 

Bacillus thuringiensis Cry35Ab1 protein in DAS-59122-7 

and the genetic material necessary for its production in corn..............................................................<0.000161%* 

Inert Ingredient:

A marker protein and the genetic material 

necessary for its production in event DAS-59122-7.........................................................................................0.0%* 

* Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA REG NO.: 29964-4 

EPA Establishment Number: 29964-IA-001 Pioneer Hi-Bred International, Inc. 

 7300 NW 62nd Ave. 

 Johnston, IA 50131 

NET CONTENTS: _______________ pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed 

may only be used according to the field protocols as specified in the approved EUP program. Participants 

and Cooperators must have a copy of each applicable protocol prior to initiating any research with these 

seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened 

coleopteran species as listed by the US Fish and Wildlife Service. 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any 

seed not used in these experiments must be returned to the seed provider or disposed of as specified in the 

field protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds 

before destroying. 

PHI-2008-139 

April 20, 2009Page 141 of 416
6) MON810 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with 

activity against certain lepidopteran pests: Cry1Ab. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the 

terms and conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the 

user at the time the corn seed is planted. 

Active Ingredient:

Bacillus thuringiensis Cry1Ab protein in MON-ØØ81Ø-6 

and the genetic material necessary for its production in corn.................................................................<0.0070%* 

* Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA REG NO.: 524-489 

EPA Establishment Number: 524-MO-002 Monsanto Company. 

 700 Chesterfield Parkway North. 

 St. Louis, Missouri 63198 

NET CONTENTS: _______________ pounds of corn seed 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed 

may only be used according to the field protocols as specified in the approved EUP program. Participants 

and Cooperators must have a copy of each applicable protocol prior to initiating any research with these 

seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened 

lepidopteran species as listed by the US Fish and Wildlife Service. 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any 

seed not used in these experiments must be returned to the seed provider or disposed of as specified in the 

field protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds 

before destroying. 

PHI-2008-139 

April 20, 2009Page 142 of 416
7) 1507 x 59122 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with 

activity against certain lepidopteran and corn rootworm pests: Cry1F and Cry34/35Ab1. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the 

terms and conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the 

user at the time the corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry1F protein in DAS-Ø15Ø7-1 

and the genetic material necessary for its production in corn................................................................<0.00038%* 

Bacillus thuringiensis Cry34Ab1 protein in DAS-59122-7 

and the genetic material necessary for its production in corn................................................................<0.00633%* 

Bacillus thuringiensis Cry35Ab1 protein in DAS-59122-7 

and the genetic material necessary for its production in corn................................................................<0.00034%* 

Inert Ingredient:

A marker protein and the genetic material 

necessary for its production in DAS-Ø15Ø7-1 & DAS-59122-7 corn.....................................................<0.00004%* 

* Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA REG NO.: 29964-5 

EPA Establishment Number: 29964-IA-001 Pioneer Hi-Bred International, Inc. 

 7300 NW 62nd Ave. 

 Johnston, IA 50131 

NET CONTENTS: _______________ pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed 

may only be used according to the field protocols as specified in the approved EUP program. Participants 

and Cooperators must have a copy of each applicable protocol prior to initiating any research with these 

seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened 

lepidopteran and coleopteran species as listed by the US Fish and Wildlife Service. 

PHI-2008-139 

April 20, 2009Page 143 of 416
STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any 

seed not used in these experiments must be returned to the seed provider or disposed of as specified in the 

field protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds 

before destroying. 

PHI-2008-139 

April 20, 2009Page 144 of 416
8) MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with 

activity against corn rootworm pests: mCry3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the 

terms and conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the 

user at the time the corn seed is planted. 

Active Ingredient:

Bacillus thuringiensis mCry3A protein in SYN-IR6Ø4-5 

and the genetic material necessary for its production in corn.......................................................<0.000000313%* 

Inert Ingredient:

A marker protein and the genetic material 

necessary for its production in event SYN-IR6Ø4-5 ..............................................................................<0.00060%* 

* Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA REG NO.: 67979-5 

EPA Establishment Number: 66736-NC-001 Syngenta Seeds Inc. 

 P.O. Box 12257 

3054 East Cornwallis Rd. 

 Research Triangle Park, NC 27709 

NET CONTENTS: _______________ pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed 

may only be used according to the field protocols as specified in the approved EUP program. Participants 

and Cooperators must have a copy of each applicable protocol prior to initiating any research with these 

seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened 

coleopteran species as listed by the US Fish and Wildlife Service. 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any 

seed not used in these experiments must be returned to the seed provider or disposed of as specified in the 

field protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds 

before destroying. 

PHI-2008-139 

April 20, 2009Page 145 of 416
9) Bt11 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with 

activity against certain lepidopteran pests: Cry1Ab. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the 

terms and conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the 

user at the time the corn seed is planted. 

Active Ingredient:

Bacillus thuringiensis Cry1Ab protein in SYN-BTØ11-1 

and the genetic material necessary for its production in corn .................................................................<0.0016%* 

Inert Ingredient:

A marker protein and the genetic material 

necessary for its production in event SYN-BTØ11-1 ...........................................................................<0.000004%* 

* Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA REG NO.: 67979-1 

EPA Establishment Number: 66736-NC-001 Syngenta Seeds Inc. 

 P.O. Box 12257 

3054 East Cornwallis Rd. 

 Research Triangle Park, NC 27709 

NET CONTENTS: _______________ pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed 

may only be used according to the field protocols as specified in the approved EUP program. Participants 

and Cooperators must have a copy of each applicable protocol prior to initiating any research with these 

seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened 

lepidopteran species as listed by the US Fish and Wildlife Service. 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any 

seed not used in these experiments must be returned to the seed provider or disposed of as specified in the 

field protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds 

before destroying. 

PHI-2008-139 

April 20, 2009Page 146 of 416
10) Bt11 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with 

activity against certain lepidopteran and corn rootworm pests: Cry1Ab and mCry3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the 

terms and conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the 

user at the time the corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry1Ab protein in SYN-BTØ11-1 

and the genetic material necessary for its production in corn................................................................<0.00019%* 

Bacillus thuringiensis mCry3A protein in SYN-IR6Ø4-5 

and the genetic material necessary for its production in corn........................................................<0.000000008%* 

Inert Ingredients:

A marker protein and the genetic material 

necessary for its production in event SYN-BTØ11-1 ...........................................................................<0.000004%* 

A marker protein and the genetic material 

necessary for its production in event SYN-IR6Ø4-5 ..............................................................................<0.00004%* 

* Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA REG NO: 67979-8 

EPA Establishment Number: 66736-NC-001 Syngenta Seeds Inc. 

 P.O. Box 12257 

3054 East Cornwallis Rd. 

 Research Triangle Park, NC 27709 

NET CONTENTS: _______________ pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed 

may only be used according to the field protocols as specified in the approved EUP program. Participants 

and Cooperators must have a copy of each applicable protocol prior to initiating any research with these 

seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened 

lepidopteran and coleopteran species as listed by the US Fish and Wildlife Service. 

PHI-2008-139 

April 20, 2009Page 147 of 416
STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any 

seed not used in these experiments must be returned to the seed provider or disposed of as specified in the 

field protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds 

before destroying. 

PHI-2008-139 

April 20, 2009Page 148 of 416
11) MIR162 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with 

activity against certain lepidopteran pests: VIP3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the 

terms and conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the 

user at the time the corn seed is planted. 

Active Ingredient:

Bacillus thuringiensis Vip3Aa20 protein in SYN-IR162-4 

and the genetic material necessary for its production in corn................................................................<0.00403%* 

Inert Ingredient:

A marker protein and the genetic material 

necessary for its production in event SYN-IR162-4...............................................................................<0.00019%* 

* Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 66736-NC-001 Syngenta Seeds Inc. 

 P.O. Box 12257 

3054 East Cornwallis Rd. 

. Research Triangle Park, NC 27709 

NET CONTENTS: ___________ pounds of corn seed 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed 

may only be used according to the field protocols as specified in the approved EUP program. Participants 

and Cooperators must have a copy of each applicable protocol prior to initiating any research with these 

seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened 

lepidopteran species as listed by the US Fish and Wildlife Service. 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any 

seed not used in these experiments must be returned to the seed provider or disposed of as specified in the 

field protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds 

before destroying. 

PHI-2008-139 

April 20, 2009Page 149 of 416
12) Bt11 x MIR162 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with 

activity against certain lepidopteran pests: Cry1Ab and VIP3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the 

terms and conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the 

user at the time the corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry1Ab protein in SYN-BTØ11-1 

and the genetic material necessary for its production in corn..................................................................<0.0011%* 

Bacillus thuringiensis Vip3Aa20 protein in SYN-IR162-4 

and the genetic material necessary for its production in corn..................................................................<0.0102%* 

Inert Ingredients:

A marker protein and the genetic material 

necessary for its production in event SYN-BTØ11-1 ...........................................................................<0.000041%* 

A marker protein and the genetic material 

necessary for its production in event SYN-IR162-4.............................................................................<0.000041%* 

* Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 66736-NC-001 Syngenta Seeds Inc. 

 P.O. Box 12257 

3054 East Cornwallis Rd. 

 Research Triangle Park, NC 27709 

NET CONTENTS: _______________ pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed 

may only be used according to the field protocols as specified in the approved EUP program. Participants 

and Cooperators must have a copy of each applicable protocol prior to initiating any research with these 

seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened 

lepidopteran species as listed by the US Fish and Wildlife Service. 

PHI-2008-139 

April 20, 2009Page 150 of 416
STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any 

seed not used in these experiments must be returned to the seed provider or disposed of as specified in the 

field protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds 

before destroying. 

PHI-2008-139 

April 20, 2009Page 151 of 416
13) Bt11 x MIR162 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with 

activity against certain lepidopteran and corn rootworm pests: Cry1Ab, VIP3A and mCry3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the 

terms and conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the 

user at the time the corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry1Ab protein in SYN-BTØ11-1 

and the genetic material necessary for its production in corn................................................................<0.00025%* 

Bacillus thuringiensis Vip3Aa20 protein in SYN-IR162-4 

and the genetic material necessary for its production in corn..................................................................<0.0165%* 

Bacillus thuringiensis mCry3A protein in SYN-IR6Ø4-5 

and the genetic material necessary for its production in corn......................................................<0.0000000077%* 

Inert Ingredients:

A marker protein and the genetic material 

necessary for its production in event SYN-BTØ11-1 .............................................................................<0.00003%* 

A marker protein and the genetic material 

necessary for its production in event SYN-IR162-4 & SYN-IR6Ø4-5.....................................................<0.00065%* 

* Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 66736-NC-001 Syngenta Seeds Inc. 

 P.O. Box 12257 

3054 East Cornwallis Rd. 

 Research Triangle Park, NC 27709 

NET CONTENTS: _______________ pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed 

may only be used according to the field protocols as specified in the approved EUP program. Participants 

and Cooperators must have a copy of each applicable protocol prior to initiating any research with these 

seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened 

lepidopteran and coleopteran species as listed by the US Fish and Wildlife Service. 

PHI-2008-139 

April 20, 2009Page 152 of 416
STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any 

seed not used in these experiments must be returned to the seed provider or disposed of as specified in the 

field protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds 

before destroying. 

PHI-2008-139 

April 20, 2009Page 153 of 416
14) 1507 x 59122 x MON810 x MIR162 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with 

activity against certain lepidopteran and corn rootworm pests: Cry1F, Cry34/35Ab1, Cry1Ab and VIP3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the 

terms and conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the 

user at the time the corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry1F protein in DAS-Ø15Ø7-1 

and the genetic material necessary for its production in corn................................................................<0.01415%* 

Bacillus thuringiensis Cry34Ab1 protein in DAS-59122-7 

and the genetic material necessary for its production in corn................................................................ <0.0117%** 

Bacillus thuringiensis Cry35Ab1 protein in DAS-59122-7 

and the genetic material necessary for its production in corn............................................................ <0.000161%** 

Bacillus thuringiensis Cry1Ab protein in MON-ØØ81Ø-6 

and the genetic material necessary for its production in corn............................................................... <0.0070%** 

Bacillus thuringiensis Vip3Aa20 protein in SYN-IR162-4 

and the genetic material necessary for its production in corn.............................................................. <0.00403%** 

Inert Ingredients:

A marker protein and the genetic material 

necessary for its production in event DAS-Ø15Ø7-1 & DAS-59122-7.............................................................0.0%* 

A marker protein and the genetic material 

necessary for its production in event SYN-IR162-4............................................................................. <0.00019%** 

* Maximum percent (wt/wt) of total extractable protein in grain 

** Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Pioneer Hi-Bred International, Inc. 

 7300 NW 62nd Ave. 

 Johnston, IA 50131 

NET CONTENTS: ___________pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed 

may only be used according to the field protocols as specified in the approved EUP program. Participants 

and Cooperators must have a copy of each applicable protocol prior to initiating any research with these 

seeds. 

PHI-2008-139 

April 20, 2009Page 154 of 416
Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened 

lepidopteran and coleopteran species as listed by the US Fish and Wildlife Service. 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any 

seed not used in these experiments must be returned to the seed provider or disposed of as specified in the 

field protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds 

before destroying. 

PHI-2008-139 

April 20, 2009Page 155 of 416
15) 1507 x 59122 x MON810 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with 

activity against certain lepidopteran and corn rootworm pests: Cry1F, Cry34/35Ab1, Cry1Ab and mCry3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the 

terms and conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the 

user at the time the corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry1F protein in DAS-Ø15Ø7-1 

and the genetic material necessary for its production in corn................................................................<0.01415%* 

Bacillus thuringiensis Cry34Ab1 protein in DAS-59122-7 

and the genetic material necessary for its production in corn................................................................ <0.0117%** 

Bacillus thuringiensis Cry35Ab1 protein in DAS-59122-7 

and the genetic material necessary for its production in corn............................................................ <0.000161%** 

Bacillus thuringiensis Cry1Ab protein in MON-ØØ81Ø-6 

and the genetic material necessary for its production in corn............................................................... <0.0070%** 

Bacillus thuringiensis mCry3A protein in SYN-IR6Ø4-5 

and the genetic material necessary for its production in corn...................................................... <0.000000313%** 

Inert Ingredients:

A marker protein and the genetic material 

necessary for its production in event DAS-Ø15Ø7-1 & DAS-59122-7.............................................................0.0%* 

A marker protein and the genetic material 

necessary for its production in event SYN-IR6Ø4-5 ............................................................................ <0.00019%** 

* Maximum percent (wt/wt) of total extractable protein in grain 

** Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Pioneer Hi-Bred International, Inc. 

 7300 NW 62nd Ave. 

 Johnston, IA 50131 

NET CONTENTS: ___________pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed 

may only be used according to the field protocols as specified in the approved EUP program. Participants 

and Cooperators must have a copy of each applicable protocol prior to initiating any research with these 

seeds. 

PHI-2008-139 

April 20, 2009Page 156 of 416
Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened 

lepidopteran and coleopteran species as listed by the US Fish and Wildlife Service. 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any 

seed not used in these experiments must be returned to the seed provider or disposed of as specified in the 

field protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds 

before destroying. 

PHI-2008-139 

April 20, 2009Page 157 of 416
16) 1507 x 59122 x MIR162 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with 

activity against certain lepidopteran and corn rootworm pests: Cry1F, Cry34/35Ab1, VIP3A and mCry3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the 

terms and conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the 

user at the time the corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry1F protein in DAS-Ø15Ø7-1 

and the genetic material necessary for its production in corn................................................................<0.01415%* 

Bacillus thuringiensis Cry34Ab1 protein in DAS-59122-7 

and the genetic material necessary for its production in corn................................................................ <0.0117%** 

Bacillus thuringiensis Cry35Ab1 protein in DAS-59122-7 

and the genetic material necessary for its production in corn............................................................ <0.000161%** 

Bacillus thuringiensis Vip3Aa20 protein in SYN-IR162-4 

and the genetic material necessary for its production in corn ............................................................. <0.00403%** 

Bacillus thuringiensis mCry3A protein in SYN-IR6Ø4-5 

and the genetic material necessary for its production in corn...................................................... <0.000000313%** 

Inert Ingredients:

A marker protein and the genetic material 

necessary for its production in DAS-Ø15Ø7-1 & DAS-59122-7 corn...............................................................0.0%* 

A marker protein and the genetic material 

necessary for its production in event SYN-IR6Ø4-5 & SYN-IR162-4 corn.......................................... <0.00019%** 

* Maximum percent (wt/wt) of total extractable protein in grain 

** Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Pioneer Hi-Bred International, Inc. 

 7300 NW 62nd Ave. 

 Johnston, IA 50131 

NET CONTENTS: ___________pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed 

may only be used according to the field protocols as specified in the approved EUP program. Participants 

and Cooperators must have a copy of each applicable protocol prior to initiating any research with these 

seeds. 

PHI-2008-139 

April 20, 2009Page 158 of 416
Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened 

lepidopteran and coleopteran species as listed by the US Fish and Wildlife Service. 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any 

seed not used in these experiments must be returned to the seed provider or disposed of as specified in the 

field protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds 

before destroying. 

PHI-2008-139 

April 20, 2009Page 159 of 416
17) 1507 x MON810 x MIR162 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with 

activity against certain lepidopteran and corn rootworm pests: Cry1F, Cry1Ab, VIP3A and mCry3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the 

terms and conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the 

user at the time the corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry1F protein in DAS-Ø15Ø7-1 

and the genetic material necessary for its production in corn................................................................<0.01415%* 

Bacillus thuringiensis Cry1Ab protein in MON-ØØ81Ø-6 

and the genetic material necessary for its production in corn............................................................... <0.0070%** 

Bacillus thuringiensis Vip3Aa20 protein in SYN-IR162-4 

and the genetic material necessary for its production in corn.............................................................. <0.00403%** 

Bacillus thuringiensis mCry3A protein in SYN-IR6Ø4-5 

and the genetic material necessary for its production in corn...................................................... <0.000000313%** 

Inert Ingredients:

A marker protein and the genetic material 

necessary for its production in event DAS-Ø15Ø7-1.......................................................................................0.0%* 

A marker protein and the genetic material 

necessary for its production in event SYN-IR6Ø4-5 & SYN-IR162-4 .................................................. <0.00019%** 

* Maximum percent (wt/wt) of total extractable protein in grain 

** Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Pioneer Hi-Bred International, Inc. 

 7300 NW 62nd Ave. 

 Johnston, IA 50131 

NET CONTENTS: ___________pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed 

may only be used according to the field protocols as specified in the approved EUP program. Participants 

and Cooperators must have a copy of each applicable protocol prior to initiating any research with these 

seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened 

lepidopteran and coleopteran species as listed by the US Fish and Wildlife Service. 

PHI-2008-139 

April 20, 2009Page 160 of 416
STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any 

seed not used in these experiments must be returned to the seed provider or disposed of as specified in the 

field protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds 

before destroying. 

PHI-2008-139 

April 20, 2009Page 161 of 416
18) 59122 x MON810 x MIR162 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with 

activity against certain lepidopteran and corn rootworm pests: Cry34/35Ab1, Cry1Ab, VIP3A and mCry3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the 

terms and conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the 

user at the time the corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry34Ab1 protein in DAS-59122-7 

and the genetic material necessary for its production in corn..................................................................<0.0117%* 

Bacillus thuringiensis Cry35Ab1 protein in DAS-59122-7 

and the genetic material necessary for its production in corn..............................................................<0.000161%* 

Bacillus thuringiensis Cry1Ab protein in MON-ØØ81Ø-6 

and the genetic material necessary for its production in corn.................................................................<0.0070%* 

Bacillus thuringiensis Vip3Aa20 protein in SYN-IR162-4 

and the genetic material necessary for its production in corn................................................................<0.00403%* 

Bacillus thuringiensis mCry3A protein in SYN-IR6Ø4-5 

and the genetic material necessary for its production in corn........................................................<0.000000313%* 

Inert Ingredients:

A marker protein and the genetic material 

necessary for its production in event DAS-59122-7.........................................................................................0.0%* 

A marker protein and the genetic material 

necessary for its production in event SYN-IR6Ø4-5 & SYN-IR162-4....................................................<0.00060%* 

* Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Pioneer Hi-Bred International, Inc. 

 7300 NW 62nd Ave. 

 Johnston, IA 50131 

NET CONTENTS: ___________pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed 

may only be used according to the field protocols as specified in the approved EUP program. Participants 

and Cooperators must have a copy of each applicable protocol prior to initiating any research with these 

seeds. 

PHI-2008-139 

April 20, 2009Page 162 of 416
Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened 

lepidopteran and coleopteran species as listed by the US Fish and Wildlife Service. 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any 

seed not used in these experiments must be returned to the seed provider or disposed of as specified in the 

field protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds 

before destroying. 

PHI-2008-139 

April 20, 2009Page 163 of 416
19) 1507 x 59122 x MIR162 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with 

activity against certain lepidopteran and corn rootworm pests: Cry1F, Cry34/35Ab1, and VIP3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the 

terms and conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the 

user at the time the corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry1F protein in DAS-Ø15Ø7-1 

and the genetic material necessary for its production in corn................................................................<0.01415%* 

Bacillus thuringiensis Cry34Ab1 protein in DAS-59122-7 

and the genetic material necessary for its production in corn................................................................ <0.0117%** 

Bacillus thuringiensis Cry35Ab1 protein in DAS-59122-7 

and the genetic material necessary for its production in corn............................................................ <0.000161%** 

Bacillus thuringiensis Vip3Aa20 protein in SYN-IR162-4 

and the genetic material necessary for its production in corn.............................................................. <0.00403%** 

Inert Ingredients:

A marker protein and the genetic material 

necessary for its production in DAS-Ø15Ø7-1 & DAS-59122-7 corn...............................................................0.0%* 

A marker protein and the genetic material 

necessary for its production in event SYN-IR162-4............................................................................. <0.00019%** 

* Maximum percent (wt/wt) of total extractable protein in grain 

** Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Pioneer Hi-Bred International, Inc. 

 7300 NW 62nd Ave. 

 Johnston, IA 50131 

NET CONTENTS: ___________pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed 

may only be used according to the field protocols as specified in the approved EUP program. Participants 

and Cooperators must have a copy of each applicable protocol prior to initiating any research with these 

seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened 

lepidopteran and coleopteran species as listed by the US Fish and Wildlife Service. 

PHI-2008-139 

April 20, 2009Page 164 of 416
STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any 

seed not used in these experiments must be returned to the seed provider or disposed of as specified in the 

field protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds 

before destroying 

PHI-2008-139 

April 20, 2009Page 165 of 416
20) 1507 x 59122 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with 

activity against certain lepidopteran and corn rootworm pests: Cry1F, Cry34/35Ab1 and mCry3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the 

terms and conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the 

user at the time the corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry1F protein in DAS-Ø15Ø7-1 

and the genetic material necessary for its production in corn................................................................<0.01415%* 

Bacillus thuringiensis Cry34Ab1 protein in DAS-59122-7 

and the genetic material necessary for its production in corn................................................................ <0.0117%** 

Bacillus thuringiensis Cry35Ab1 protein in DAS-59122-7 

and the genetic material necessary for its production in corn............................................................ <0.000161%** 

Bacillus thuringiensis mCry3A protein in SYN-IR6Ø4-5 

and the genetic material necessary for its production in corn...................................................... <0.000000313%** 

Inert Ingredients:

A marker protein and the genetic material 

necessary for its production in DAS-Ø15Ø7-1 & DAS-59122-7 corn...............................................................0.0%* 

A marker protein and the genetic material 

necessary for its production in event SYN-IR6Ø4-5 ............................................................................ <0.00060%** 

* Maximum percent (wt/wt) of total extractable protein in grain 

** Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Pioneer Hi-Bred International, Inc. 

 7300 NW 62nd Ave. 

 Johnston, IA 50131 

NET CONTENTS: ___________pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed 

may only be used according to the field protocols as specified in the approved EUP program. Participants 

and Cooperators must have a copy of each applicable protocol prior to initiating any research with these 

seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened 

lepidopteran and coleopteran species as listed by the US Fish and Wildlife Service. 

PHI-2008-139 

April 20, 2009Page 166 of 416
STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any 

seed not used in these experiments must be returned to the seed provider or disposed of as specified in the 

field protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds 

before destroying. 

PHI-2008-139 

April 20, 2009Page 167 of 416
21) 1507 x MON810 x MIR162 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with 

activity against certain lepidopteran pests: Cry1F, Cry1Ab and VIP3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the 

terms and conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the 

user at the time the corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry1F protein in DAS-Ø15Ø7-1 

and the genetic material necessary for its production in corn................................................................<0.01415%* 

Bacillus thuringiensis Cry1Ab protein in MON-ØØ81Ø-6 

and the genetic material necessary for its production in corn............................................................... <0.0070%** 

Bacillus thuringiensis Vip3Aa20 protein in SYN-IR162-4 

and the genetic material necessary for its production in corn.............................................................. <0.00403%** 

Inert Ingredients:

A marker protein and the genetic material 

necessary for its production in event DAS-Ø15Ø7-1...............................................................................<0.0020%* 

A marker protein and the genetic material 

necessary for its production in event SYN-IR162-4............................................................................. <0.00019%** 

* Maximum percent (wt/wt) of total extractable protein in grain 

** Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Pioneer Hi-Bred International, Inc. 

 7300 NW 62nd Ave. 

 Johnston, IA 50131 

NET CONTENTS: ___________pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed 

may only be used according to the field protocols as specified in the approved EUP program. Participants 

and Cooperators must have a copy of each applicable protocol prior to initiating any research with these 

seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened 

lepidopteran species as listed by the US Fish and Wildlife Service. 

PHI-2008-139 

April 20, 2009Page 168 of 416
STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any 

seed not used in these experiments must be returned to the seed provider or disposed of as specified in the 

field protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds 

before destroying 

PHI-2008-139 

April 20, 2009Page 169 of 416
22) 1507 x MON810 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with 

activity against certain lepidopteran and corn rootworm pests: Cry1F, Cry1Ab and mCry3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the 

terms and conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the 

user at the time the corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry1F protein in DAS-Ø15Ø7-1 

and the genetic material necessary for its production in corn................................................................<0.01415%* 

Bacillus thuringiensis Cry1Ab protein in MON-ØØ81Ø-6 

and the genetic material necessary for its production in corn............................................................... <0.0070%** 

Bacillus thuringiensis mCry3A protein in SYN-IR6Ø4-5 

and the genetic material necessary for its production in corn...................................................... <0.000000313%** 

Inert Ingredients:

A marker protein and the genetic material 

necessary for its production in event DAS-Ø15Ø7-1...............................................................................<0.0020%* 

A marker protein and the genetic material 

necessary for its production in event SYN-IR6Ø4-5 ............................................................................ <0.00060%** 

* Maximum percent (wt/wt) of total extractable protein in grain 

** Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Pioneer Hi-Bred International, Inc. 

 7300 NW 62nd Ave. 

 Johnston, IA 50131 

NET CONTENTS: ___________pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed 

may only be used according to the field protocols as specified in the approved EUP program. Participants 

and Cooperators must have a copy of each applicable protocol prior to initiating any research with these 

seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened 

lepidopteran and coleopteran species as listed by the US Fish and Wildlife Service. 

PHI-2008-139 

April 20, 2009Page 170 of 416
STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any 

seed not used in these experiments must be returned to the seed provider or disposed of as specified in the 

field protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds 

before destroying. 

PHI-2008-139 

April 20, 2009Page 171 of 416
23) 1507 x MIR162 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with 

activity against certain lepidopteran and corn rootworm pests: Cry1F, VIP3A and mCry3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the 

terms and conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the 

user at the time the corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry1F protein in DAS-Ø15Ø7-1 

and the genetic material necessary for its production in corn................................................................<0.01415%* 

Bacillus thuringiensis Vip3Aa20 protein in SYN-IR162-4 

and the genetic material necessary for its production in corn.............................................................. <0.00403%** 

Bacillus thuringiensis mCry3A protein in SYN-IR6Ø4-5 

and the genetic material necessary for its production in corn...................................................... <0.000000313%** 

Inert Ingredients:

A marker protein and the genetic material 

necessary for its production in event DAS-Ø15Ø7-1.......................................................................................0.0%* 

A marker protein and the genetic material 

necessary for its production in event SYN-IR6Ø4-5 & SYN-IR162-4................................................... <0.00019%** 

* Maximum percent (wt/wt) of total extractable protein in grain 

** Maximum percent (wt/wt) of dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Pioneer Hi-Bred International, Inc. 

 7300 NW 62nd Ave. 

 Johnston, IA 50131 

NET CONTENTS: ___________pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed 

may only be used according to the field protocols as specified in the approved EUP program. Participants 

and Cooperators must have a copy of each applicable protocol prior to initiating any research with these 

seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened 

lepidopteran and coleopteran species as listed by the US Fish and Wildlife Service. 

PHI-2008-139 

April 20, 2009Page 172 of 416
STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any 

seed not used in these experiments must be returned to the seed provider or disposed of as specified in the 

field protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds 

before destroying. 

PHI-2008-139 

April 20, 2009Page 173 of 416
24) 59122 x MON810 x MIR162 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with 

activity against certain lepidopteran and corn rootworm pests: Cry34/35Ab1, Cry1Ab and VIP3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the 

terms and conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the 

user at the time the corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry34Ab1 protein in DAS-59122-7 

and the genetic material necessary for its production in corn..................................................................<0.0117%* 

Bacillus thuringiensis Cry35Ab1 protein in DAS-59122-7 

and the genetic material necessary for its production in corn..............................................................<0.000161%* 

Bacillus thuringiensis Cry1Ab protein in MON-ØØ81Ø-6 

and the genetic material necessary for its production in corn.................................................................<0.0070%* 

Bacillus thuringiensis Vip3Aa20 protein in SYN-IR162-4 

and the genetic material necessary for its production in corn................................................................<0.00403%* 

Inert Ingredients:

A marker protein and the genetic material 

necessary for its production in event DAS-59122-7.........................................................................................0.0%* 

A marker protein and the genetic material 

necessary for its production in event SYN-IR162-4...............................................................................<0.00019%* 

* Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Pioneer Hi-Bred International, Inc. 

 7300 NW 62nd Ave. 

 Johnston, IA 50131 

NET CONTENTS: ___________pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed 

may only be used according to the field protocols as specified in the approved EUP program. Participants 

and Cooperators must have a copy of each applicable protocol prior to initiating any research with these 

seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened 

lepidopteran and coleopteran species as listed by the US Fish and Wildlife Service. 

PHI-2008-139 

April 20, 2009Page 174 of 416
STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any 

seed not used in these experiments must be returned to the seed provider or disposed of as specified in the 

field protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds 

before destroying. 

PHI-2008-139 

April 20, 2009Page 175 of 416
25) 59122 x MON810 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with 

activity against certain lepidopteran and corn rootworm pests: Cry34/35Ab1, Cry1Ab and mCry3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the 

terms and conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the 

user at the time the corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry34Ab1 protein in DAS-59122-7 

and the genetic material necessary for its production in corn..................................................................<0.0117%* 

Bacillus thuringiensis Cry35Ab1 protein in DAS-59122-7 

and the genetic material necessary for its production in corn..............................................................<0.000161%* 

Bacillus thuringiensis Cry1Ab protein in MON-ØØ81Ø-6 

and the genetic material necessary for its production in corn.................................................................<0.0070%* 

Bacillus thuringiensis mCry3A protein in SYN-IR6Ø4-5 

and the genetic material necessary for its production in corn........................................................<0.000000313%* 

Inert Ingredients:

A marker protein and the genetic material 

necessary for its production in event DAS-59122-7.........................................................................................0.0%* 

A marker protein and the genetic material 

necessary for its production in event SYN-IR6Ø4-5 ..............................................................................<0.00060%* 

* Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Pioneer Hi-Bred International, Inc. 

 7300 NW 62nd Ave. 

 Johnston, IA 50131 

NET CONTENTS: ___________pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed 

may only be used according to the field protocols as specified in the approved EUP program. Participants 

and Cooperators must have a copy of each applicable protocol prior to initiating any research with these 

seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened 

lepidopteran and coleopteran species as listed by the US Fish and Wildlife Service. 

PHI-2008-139 

April 20, 2009Page 176 of 416
STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any 

seed not used in these experiments must be returned to the seed provider or disposed of as specified in the 

field protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds 

before destroying. 

PHI-2008-139 

April 20, 2009Page 177 of 416
26) 59122 x MIR162 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with 

activity against certain lepidopteran and corn rootworm pests: Cry34/35Ab1, VIP3A and mCry3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the 

terms and conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the 

user at the time the corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry34Ab1 protein in DAS-59122-7 

and the genetic material necessary for its production in corn..................................................................<0.0117%* 

Bacillus thuringiensis Cry35Ab1 protein in DAS-59122-7 

and the genetic material necessary for its production in corn..............................................................<0.000161%* 

Bacillus thuringiensis Vip3Aa20 protein in SYN-IR162-4 

and the genetic material necessary for its production in corn................................................................<0.00403%* 

Bacillus thuringiensis mCry3A protein in SYN-IR6Ø4-5 

and the genetic material necessary for its production in corn........................................................<0.000000313%* 

Inert Ingredients:

A marker protein and the genetic material 

necessary for its production in event DAS-59122-7.........................................................................................0.0%* 

A marker protein and the genetic material 

necessary for its production in event SYN-IR6Ø4-5 & SYN-IR162-4.....................................................<0.00019%* 

* Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Pioneer Hi-Bred International, Inc. 

 7300 NW 62nd Ave. 

 Johnston, IA 50131 

NET CONTENTS: ___________pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed 

may only be used according to the field protocols as specified in the approved EUP program. Participants 

and Cooperators must have a copy of each applicable protocol prior to initiating any research with these 

seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened 

lepidopteran and coleopteran species as listed by the US Fish and Wildlife Service. 

PHI-2008-139 

April 20, 2009Page 178 of 416
STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any 

seed not used in these experiments must be returned to the seed provider or disposed of as specified in the 

field protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds 

before destroying. 

PHI-2008-139 

April 20, 2009Page 179 of 416
27) MON810 x MIR162 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with 

activity against certain lepidopteran and corn rootworm pests: Cry1Ab, VIP3A and mCry3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the 

terms and conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the 

user at the time the corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry1Ab protein in MON-ØØ81Ø-6 

and the genetic material necessary for its production in corn.................................................................<0.0070%* 

Bacillus thuringiensis Vip3Aa20 protein in SYN-IR162-4 

and the genetic material necessary for its production in corn................................................................<0.00403%* 

Bacillus thuringiensis mCry3A protein in SYN-IR6Ø4-5 

and the genetic material necessary for its production in corn........................................................<0.000000313%* 

Inert Ingredient:

A marker protein and the genetic material 

necessary for its production in event SYN-IR6Ø4-5 & SYN-IR162-4.....................................................<0.00019%* 

* Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Pioneer Hi-Bred International, Inc. 

 7300 NW 62nd Ave. 

 Johnston, IA 50131 

NET CONTENTS: ___________pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed 

may only be used according to the field protocols as specified in the approved EUP program. Participants 

and Cooperators must have a copy of each applicable protocol prior to initiating any research with these 

seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened 

lepidopteran and coleopteran species as listed by the US Fish and Wildlife Service. 

PHI-2008-139 

April 20, 2009Page 180 of 416
STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any 

seed not used in these experiments must be returned to the seed provider or disposed of as specified in the 

field protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds 

before destroying. 

PHI-2008-139 

April 20, 2009Page 181 of 416
28) 1507 x MIR162 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with 

activity against certain lepidopteran pests: Cry1F and VIP3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the 

terms and conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the 

user at the time the corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry1F protein in DAS-Ø15Ø7-1 

and the genetic material necessary for its production in corn................................................................<0.01415%* 

Bacillus thuringiensis Vip3Aa20 protein in SYN-IR162-4 

and the genetic material necessary for its production in corn.............................................................. <0.00403%** 

Inert Ingredients:

A marker protein and the genetic material 

necessary for its production in event DAS-Ø15Ø7-1..............................................................................< 0.0020%* 

A marker protein and the genetic material 

necessary for its production in event SYN-IR162-4............................................................................. <0.00019%** 

* Maximum percent (wt/wt) of total extractable protein in grain 

** Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Pioneer Hi-Bred International, Inc. 

 7300 NW 62nd Ave. 

 Johnston, IA 50131 

NET CONTENTS: ___________pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed 

may only be used according to the field protocols as specified in the approved EUP program. Participants 

and Cooperators must have a copy of each applicable protocol prior to initiating any research with these 

seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened 

lepidopteran species as listed by the US Fish and Wildlife Service. 

PHI-2008-139 

April 20, 2009Page 182 of 416
STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any 

seed not used in these experiments must be returned to the seed provider or disposed of as specified in the 

field protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds 

before destroying. 

PHI-2008-139 

April 20, 2009Page 183 of 416
29) 1507 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with 

activity against certain lepidopteran and corn rootworm pests: Cry1F and mCry3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the 

terms and conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the 

user at the time the corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry1F protein in DAS-Ø15Ø7-1 

and the genetic material necessary for its production in corn................................................................<0.01415%* 

Bacillus thuringiensis mCry3A protein in SYN-IR6Ø4-5 

and the genetic material necessary for its production in corn..................................................... < 0.000000313%** 

Inert Ingredients:

A marker protein and the genetic material 

necessary for its production in event DAS-Ø15Ø7-1.......................................................................................0.0%* 

A marker protein and the genetic material 

necessary for its production in event SYN-IR6Ø4-5 ............................................................................ <0.00060%** 

* Maximum percent (wt/wt) of total extractable protein in grain 

** Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Pioneer Hi-Bred International, Inc. 

 7300 NW 62nd Ave. 

 Johnston, IA 50131 

NET CONTENTS: ___________pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed 

may only be used according to the field protocols as specified in the approved EUP program. Participants 

and Cooperators must have a copy of each applicable protocol prior to initiating any research with these 

seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened 

lepidopteran and coleopteran species as listed by the US Fish and Wildlife Service. 

PHI-2008-139 

April 20, 2009Page 184 of 416
STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any 

seed not used in these experiments must be returned to the seed provider or disposed of as specified in the 

field protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds 

before destroying. 

PHI-2008-139 

April 20, 2009Page 185 of 416
30) 59122 x MIR162 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with 

activity against certain lepidopteran and corn rootworm pests: Cry34/35Ab1 and VIP3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the 

terms and conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the 

user at the time the corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry34Ab1 protein in DAS-59122-7 

and the genetic material necessary for its production in corn.................................................................<0.0117 %* 

Bacillus thuringiensis Cry35Ab1 protein in DAS-59122-7 

and the genetic material necessary for its production in corn..............................................................<0.000161%* 

Bacillus thuringiensis Vip3Aa20 protein in SYN-IR162-4 

and the genetic material necessary for its production in corn................................................................<0.00403%* 

Inert Ingredients:

A marker protein and the genetic material 

necessary for its production in event DAS-59122-7.........................................................................................0.0%* 

A marker protein and the genetic material 

necessary for its production in event SYN-IR162-4...............................................................................<0.00019%* 

* Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Pioneer Hi-Bred International, Inc. 

 7300 NW 62nd Ave. 

 Johnston, IA 50131 

NET CONTENTS: ___________pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed 

may only be used according to the field protocols as specified in the approved EUP program. Participants 

and Cooperators must have a copy of each applicable protocol prior to initiating any research with these 

seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened 

lepidopteran and coleopteran species as listed by the US Fish and Wildlife Service. 

PHI-2008-139 

April 20, 2009Page 186 of 416
STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any 

seed not used in these experiments must be returned to the seed provider or disposed of as specified in the 

field protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds 

before destroying. 

PHI-2008-139 

April 20, 2009Page 187 of 416
31) 59122 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with 

activity against corn rootworm pests: Cry34/35Ab1 and mCry3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the 

terms and conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the 

user at the time the corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry34Ab1 protein in DAS-59122-7 

and the genetic material necessary for its production in corn..................................................................<0.0117%* 

Bacillus thuringiensis Cry35Ab1 protein in DAS-59122-7 

and the genetic material necessary for its production in corn..............................................................<0.000161%* 

Bacillus thuringiensis mCry3A protein in SYN-IR6Ø4-5 

and the genetic material necessary for its production in corn........................................................<0.000000313%* 

Inert Ingredients:

A marker protein and the genetic material 

necessary for its production in event DAS-59122-7.........................................................................................0.0%* 

A marker protein and the genetic material 

necessary for its production in event SYN-IR6Ø4-5 ..............................................................................<0.00060%* 

* Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Pioneer Hi-Bred International, Inc. 

 7300 NW 62nd Ave. 

 Johnston, IA 50131 

NET CONTENTS: ___________pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed 

may only be used according to the field protocols as specified in the approved EUP program. Participants 

and Cooperators must have a copy of each applicable protocol prior to initiating any research with these 

seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened 

coleopteran species as listed by the US Fish and Wildlife Service. 

PHI-2008-139 

April 20, 2009Page 188 of 416
STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any 

seed not used in these experiments must be returned to the seed provider or disposed of as specified in the 

field protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds 

before destroying. 

PHI-2008-139 

April 20, 2009Page 189 of 416
32) MON810 x MIR162 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with 

activity against certain lepidopteran pests: Cry1Ab and VIP3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the 

terms and conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the 

user at the time the corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry1Ab protein in MON-ØØ81Ø-6 

and the genetic material necessary for its production in corn.................................................................<0.0070%* 

Bacillus thuringiensis Vip3Aa20 protein in SYN-IR162-4 

and the genetic material necessary for its production in corn................................................................<0.00403%* 

Inert Ingredient:

A marker protein and the genetic material 

necessary for its production in event SYN-IR162-4...............................................................................<0.00019%* 

* Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Pioneer Hi-Bred International, Inc. 

 7300 NW 62nd Ave. 

 Johnston, IA 50131 

NET CONTENTS: ___________pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed 

may only be used according to the field protocols as specified in the approved EUP program. Participants 

and Cooperators must have a copy of each applicable protocol prior to initiating any research with these 

seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened 

lepidopteran species as listed by the US Fish and Wildlife Service. 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any 

seed not used in these experiments must be returned to the seed provider or disposed of as specified in the 

field protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds 

before destroying. 

PHI-2008-139 

April 20, 2009Page 190 of 416
33) MON810 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with 

activity against certain lepidopteran and corn rootworm pests: Cry1Ab and mCry3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the 

terms and conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the 

user at the time the corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry1Ab protein in MON-ØØ81Ø-6 

and the genetic material (PV-ZMBK07) necessary for its production in corn..........................................<0.0070%* 

Bacillus thuringiensis mCry3A protein in SYN-IR6Ø4-5 

and the genetic material necessary for its production in corn........................................................<0.000000313%* 

Inert Ingredient:

A marker protein and the genetic material 

necessary for its production in event SYN-IR6Ø4-5 ..............................................................................<0.00060%* 

* Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Pioneer Hi-Bred International, Inc. 

 7300 NW 62nd Ave. 

 Johnston, IA 50131 

NET CONTENTS: ___________pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed 

may only be used according to the field protocols as specified in the approved EUP program. Participants 

and Cooperators must have a copy of each applicable protocol prior to initiating any research with these 

seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened 

lepidopteran and coleopteran species as listed by the US Fish and Wildlife Service. 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any 

seed not used in these experiments must be returned to the seed provider or disposed of as specified in the 

field protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds 

before destroying. 

PHI-2008-139 

April 20, 2009Page 191 of 416
34) MIR162 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with 

activity against certain lepidopteran and corn rootworm pests: VIP3A and mCry3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the 

terms and conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the 

user at the time the corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Vip3Aa20 protein in SYN-IR162-4 

and the genetic material necessary for its production in corn................................................................<0.00403%* 

Bacillus thuringiensis mCry3A protein in SYN-IR6Ø4-5 

and the genetic material necessary for its production in corn........................................................<0.000000313%* 

Inert Ingredient:

A marker protein and the genetic material 

necessary for its production in event SYN-IR6Ø4-5 & SYN-IR162-4.....................................................<0.00019%* 

* Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Pioneer Hi-Bred International, Inc. 

 7300 NW 62nd Ave. 

 Johnston, IA 50131 

NET CONTENTS: ___________pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed 

may only be used according to the field protocols as specified in the approved EUP program. Participants 

and Cooperators must have a copy of each applicable protocol prior to initiating any research with these 

seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened 

lepidopteran and coleopteran species as listed by the US Fish and Wildlife Service. 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any 

seed not used in these experiments must be returned to the seed provider or disposed of as specified in the 

field protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds 

before destroying. 

PHI-2008-139 

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PHI-2008-139 Page 190 of 237 

August 14, 2008 

 

Section C. Reference to Available Environmental Toxicity Data 

Summary of data for individual events 

59122, 1507, and MON 810 are each registered events; extensive data have been submitted to EPA 

and have been previously reviewed (U.S. EPA, 2001 & 2005). To summarize, nontarget organism 

testing was performed on each of the single events (or their respective Bt proteins) with honeybees 

(larvae and adults), Daphnia magna, lady beetles, parasitic hymenoptera, green lacewing, 

earthworms, and collembolans. No adverse effects were found at environmentally relevant exposures 

for any of the species tested. As one of the most publicly recognized insects, monarch butterfly larvae 

were also tested for sensitivity to Cry1F and Cry1Ab, and no significant risk to monarchs was found at 

environmentally relevant exposures. Additionally, environmental fate data indicate that Cry1F, 

Cry34/35Ab1 and Cry1Ab dissipate rapidly from soil. Therefore accumulation of the proteins in soil 

should be minimal, thereby decreasing the likelihood of exposure to nontarget soil organisms. The 

Agency previously concluded that the levels of Cry 1F, Cry 1Ab, and Cry 34/35Ab1 likely to occur in 

the environment will not pose unreasonable risk to nontarget organisms, including mammals, birds, 

fish, bees, and other invertebrates, based on the required and voluntary data submitted. 

Specific data for proposed stack events 

Pioneer measured the concentrations of Cry1F, Cry34/35Ab1, Cry1Ab and PAT in plant tissues of the 

proposed stacked events. Expression levels were similar to those of the single events (PHI-2008-

006), thereby indicating no increase in potential exposure concentrations compared to the previously 

reviewed individual events. 

Pioneer also evaluated the effects of pollen from the proposed stacked events on monarch butterfly 

larvae. No adverse effects were seen (PHI-2008-104 and PHI-2008-105); therefore, we do not expect 

any additional risk from the stacked products compared to single events for non-target lepidoptera.

In addition, Pioneer confirmed that the efficacy of 1507 pyramided with MON810 is additive in nature 

(as opposed to synergistic or antagonistic) against European corn borer, southwestern corn borer, 

corn earworm, and fall armyworm (PHI-2008-014). Because there was no evidence of synergy during 

testing of several key target pests, we do not expect synergy to occur in non-target insects. 

Based on the lack of environmental and mammalian effects in the individual events, the similarity in 

expression levels, the lack of synergy seen in additivity testing, and the low likelihood of exposure, we 

expect no adverse effects from the proposed stacked products to mammals, birds, fish, bees, other 

invertebrates, and plants. Lack of exposure due to the small scale of testing plots and the limited 

duration of the EUP also points to a very low likelihood of risk from the proposed stacked product, 

similar to the findings by EPA for the previously registered individual events. In addition, the EUP 

Program will be conducted on land that is currently in agricultural use. Therefore, we do not expect 

that the proposed stacked product, which may include all of these proteins, will have a different 

environmental profile than the individual products that have been previously reviewed by EPA. 

References 

U.S. EPA. 2001. Environmental Assessment: Bt Plant-Incorporated Protectants. Biopesticides 

Registration Action Document. October 15, 2001. Accessed June 24, 2008: 

http://www.epa.gov/oppbppd1/biopesticides/pips/bt_brad2/3-ecological.pdf

U.S. EPA. 2005. Environmental Assessment: Bacillus thuringiensis Cry34/35Ab1 Corn Biopesticide 

Registration Action Document. October 2005. Accessed June 24, 2008: 

http://www.epa.gov/pesticides/biopesticides/ingredients/tech_docs/brad_006490.pdf

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August 14, 2008 

 

Section D. Residue Data 

The Cry1F, Cry1Ab and Cry34/35 proteins and the genetic material necessary for their production in 

corn are plant-incorporated protectants (PIPs). These PIPs are not applied to the corn crop as 

conventional pesticides would be, but rather are synthesized by plants carrying the cry1f, Cry1Ab and 

cry34/35 genes. Residue data per se are not available for a PIP. However, for purposes of assessing 

dietary and environmental risk of 1507x59122xMON810, 1507xMON810 and 59122XMON810, the 

concentrations of Cry1F, Cry1(A)b, Cry34/35 and phosphinothricin acetyltransferase (PAT) proteins 

were determined in different plant tissues at different plant growth stages using quantitative ELISA 

assays. The results from this determination are summarized in Volume 5 of this submission (PHI2008-006). 

PHI-2008-139 

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August 14, 2008 

 

Section E. Effectiveness Data 

To date, limited efficacy data have been generated on the EUP breeding stacks listed in Table 1. 

However, the individual events are well characterized with respect to efficacy, and 1507 and MON810 

have been demonstrated to be additive in nature, as opposed to synergistic or antagonistic (PHI-2008-

114). Therefore, the breeding pyramids of 1507xMON810 and 1507x59122xMON810 are expected to 

be efficacious against the pests listed in Table 2. 

Table 2. Lepidopteran Pests That Are Controlled or Suppressed by Pyramided 1507xMON810* 

Scientific name Common name 1507 

Activity 

MON810 

Activity 

Predicted 

1507 x MON810 

Activity 

Agrotis ipsilon Black cutworm Yes No Yes 

Helicoverpa zea Corn earworm Yes Yes Yes 

Ostrinia nubilalis European corn borer Yes Yes Yes 

Spodoptera frugiperda Fall armyworm Yes Yes Yes 

Diatraea grandiosella Southwestern corn 

borer 

Yes Yes Yes 

Richia albicosta Western bean cutworm Yes No Yes 

Elasmopalpus 

lignosellus 

Lesser corn stalk borer Yes No Yes 

Diatrae crambidoides Southern corn stalk 

borer 

Yes Yes Yes 

Diatrea saccharalis Sugarcane borer Yes No Yes 

Papaipema nebris Common stalk borer No Yes Yes 

* based on current labels 

The breeding stacks of 1507x59122xMON810 and 59122xMON810 are expected to be equal in 

efficacy to the 59122 event alone against corn rootworm pests: Diabrotica virgifera virgifera, western 

corn rootworm (WCRW), Diabrotica berberi, northern corn rootworm (NCRW), and Diabrotica 

virgiferea zeae, Mexican corn rootworm (MCRW). 

Under the proposed EUP program, efficacy against lepidopteran and coleopteran pests will be 

evaluated in a range of environments, growing conditions and genetic backgrounds. 

Thus far, limited field efficacy trials have been completed characterizing first generation European corn 

borer, Ostrinia nubilalis (Hubner) (ECB), foliar feeding and fall armyworm, Spodoptera frugiperda (J.E. 

Smith) (FAW), whorl feeding on three maize hybrids containing events 1507x59122, 

1507x59122xMON810, and a negative control (isoline). The experimental design included nine 

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August 14, 2008 

 

entries (each a single-row plot with 30” row spacing) arranged in a randomized complete block with 

three replications. 

For trials characterizing first generation ECB feeding, 100 neonates/plant were manually infested three 

times within a 7-day period (300 total neonates/plant) beginning when plants reached growth stage 

V5. Approximately three weeks after the last infestation, mean damage ratings were taken on either 

10 or 15 individual plants per plot, depending on location (Table 3). 

For trials characterizing fall armyworm feeding, 20 neonates/plant were manually infested three times 

within a 10-day period (60 total neonates/plant) beginning when plants reached growth stage V4. 

Approximately two weeks after the last infestation, mean damage ratings were taken on either 10 or 

15 plants per plot, depending on location (Table 4). 

Results to date indicate that early vegetative-stage maize hybrids containing either events 

1507x59122xMON810 or events 1507x59122 were nearly immune to first generation ECB and FAW 

foliar injury. Additionally, there were no significant differences in mean damage ratings between 

hybrids containing events 1507x59122xMON810 and hybrids with only 1507x59122 for both pests at 

all locations. 

These results demonstrate the efficacy of these events is stable regardless of stacking or pyramiding 

in the event combinations named in Table 1. The EUP will allow for more extensive efficacy testing to 

further characterize the pest spectrum and level of efficacy of named event combinations under a 

wider range of environmental conditions.

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August 14, 2008 

 

Table 3. Efficacy of Breeding Stacks Against First Generation European Corn Borer 

Mean damage ratings characterizing first generation ECB foliar feeding on maize hybrids containing 

events 1507x59122, 1507x59122xMON810, or a negative control (isoline) at 5 field trial locations in 

2008. 

Location Event Combination na Mean Damage Rating 

Standard Error b,c

1507x59122 135 9.0  0.00 a 

York, NE 1507x59122xMON810 135 9.0  0.00 a 

Negative control 135 5.4  0.07 b 

1507x59122 135 9.0  0.00 a 

Dallas Center, IA 1507x59122xMON810 135 9.0  0.00 a 

Negative control 135 5.1  0.07 b 

1507x59122 90 9.0  0.00 a 

Johnston, IA 1507x59122xMON810 90 9.0  0.00 a 

Negative control 89 5.6  0.07b 

1507x59122 135 9.0  0.00 a 

Mankato, MN 1507x59122xMON810 135 9.0  0.00 a 

Negative control 135 5.8  0.09 b 

1507x59122 135 9.0  0.00 a 

Princeton, IL 1507x59122xMON810 132 9.0  0.00 a 

Negative control 135 6.0  0.08 b 

a

 n = number of plants characterized; 3 maize hybrid backgrounds, 3 replications, and either 10 or 15 

plants/plot depending on location. 

b

 Damage ratings on individual plants were determined using the following visual rating scale: 

9. No visible leaf injury or a small amount of pin or fine shot-hole type injury on a few leaves. 

8. Small amount of shot-hole type lesions on a few leaves. 

7. Shot-hole injury common on several leaves. 

6. Several leaves with shot-hole and elongated lesions (Lesions <0.5” in length). 

5. Several leaves with elongated lesions (Lesions 0.5” to 1.0” in length). 

4. Several leaves with elongated lesions (Lesions >1.0” in length). 

3. Long lesions (>1.0”) common on about one-half the leaves. 

2. Long lesions (>1.0”) common on about two-thirds the leaves. 

1. Most of the leaves with long lesions. c

 Within a location, means with the same letter are not significantly different (Fisher’s Protected LSD 

test, P > 0.05). 

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August 14, 2008 

 

Table 4. Efficacy of Breeding Stacks Against Fall Armyworm 

Mean damage ratings characterizing FAW foliar feeding on maize hybrids containing events 

1507x59122, 1507x59122xMON810, or a negative control (isoline) at 2 field trial locations in 2008. 

Location Event Combination na Mean Damage Rating 

Standard Errorb,c

1507x59122 135 8.9  0.02 a 

Dallas Center, IA 1507x59122xMON810 135 8.9  0.02 a 

Negative control 135 2.4  0.07 b 

1507x59122 90 9.0  0.00 a 

Johnston, IA 1507x59122xMON810 89 9.0  0.02 a 

Negative control 89 2.8  0.09 b 

a

 n = number of plants characterized; 3 maize hybrid backgrounds, 3 replications, and either 10 or 15 

plants/plot depending on location. 

b

 Damage ratings on individual plants were determined using the following visual rating scale: 

9. No damage to pinhole lesions present on whorl leaves. 

8. Pinholes and small circular lesions present on whorl leaves. 

7. Small circular lesions and a few small elongated (rectangular shaped) lesions up to 0.5” in 

length present on whorl and furl leaves. 

6. Several small elongated lesions 0.5” to 1” in length on a few whorl and furl leaves. 

5. Several large elongated lesions greater than 1” in length present on a few whorl and furl leaves 

and/or a few small to mid-sized uniform to irregular shaped holes (basement membrane 

consumed) in whorl and furl leaves. 

4. Several large elongated lesions present on several whorl and furl leaves and/or several large 

uniform to irregular shaped holes in whorl and furl leaves. 

3. Many elongated lesions of all sizes present on several whorl leaves plus several large uniform 

to irregular shaped holes in whorl and furl leaves. 

2. Many elongated lesions of all sizes present on most whorl and furl leaves plus many mid to 

large-sized uniform to irregular shaped holes in whorl and furl leaves. 

1. Whorl and furl leaves almost totally destroyed. c

 Within a location, means with the same letter are not significantly different (Fisher’s Protected LSD 

test, P > 0.05). 

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August 14, 2008 

 

Section F. Tolerance if Testing Involves Use in Food or Feed 

Phosphinothricin acetyltransferase (PAT), the marker protein in 1507 and 59122 corn, is exempt from 

the requirement of a tolerance in all plants: 40CFR §180.1151. 

MON810 does not contain a marker protein. 

Cry1Ab, the PIP in MON810 corn, is exempt from the requirement of a tolerance in all plants: 40CFR 

§180.1173. 

Cry1F, the PIP in 1507 corn, is exempt from the requirement of a tolerance in corn: 40CFR §180.1217. 

Cry34Ab1 and Cry35Ab1, the PIPs in 59122 corn, are exempt from the requirement of a tolerance in 

corn: 40CFR §174.457. 

PHI-2008-139 

April 20, 2009Page 199 of 416
Section G. Proposed Experimental Program 

For the adequate development of insect-protected transgenic plants, it is necessary to properly 

characterize the performance of the crop through large-scale field testing. In order to thoroughly 

evaluate corn plants, Experiments must be grown at a large number of locations, with many 

replications of each experiment, in order to obtain sufficient data for hybrid evaluation and 

advancement. Placement of Experiments with research centers and cooperators is typically not 

decided until early spring of any given year. Spring weather conditions can affect planting locations. 

Therefore, the proposed EUP acreage needs to take into account the possibility of Experiments being 

planted in a variety of locations and states. Because of this flexibility needed, the proposed acreage is 

likely an overestimate of the amount of EUP material that will actually be planted. 

G1. Participants in the Experimental Program 

Table 5 contains a list of the Participants who will supervise the work conducted under this EUP. All 

Participants are employed by Dupont/Pioneer Hi-Bred International, Inc. Participants listed below may 

supervise EUP Blocks in states other than their own (for example, if they have a cooperator across the 

state line). 

Table 5. List of Participants 

State Participant Name Participant Address Phone 

Number 

AR Mark Hood and/or 

Bob Suttner 

Pioneer Hi-Bred International Inc., 11329 Highway 

70, P.O. BOX 309, Proctor, AR 72376 870-702-7180 

CA Eduardo Traut and/or 

Salim Hakimi 

Pioneer Hi-Bred International Inc., 18285 County 

Road 96, Woodland, CA 95695 530-666-1084 

CO Chris Fagan and/or 

Don Pope 

Pioneer Hi-Bred International Inc., 4200 West 

Service Road, Unit #4, Evans, CO 8062 970-506-9219 

DE Mark Williams and/or 

Dan Mongeau 

DuPont Experimental Station, Route 141, Henry 

Clay Bldg #353, Wilmington, DE 19880 302-695-8858 

GA Dan Gorman and/or 

Joshua Stanaland 

Pioneer Hi-Bred International Inc., 2300 Industrial 

Park Road NE, Cairo, GA 39828 229-378-8240 

HI 

Bruce Heckman 

and/or Rhian 

Campbell 

Pioneer Hi-Bred International Inc., 1-385 

Kaumaualii Highway, Waimea, Kauai, HI 96796 808-338-8300 

HI Steven Kai and/or Jill 

Suga 

Pioneer Hi-Bred International Inc., 7431 Kaumaualii 

Highway, P.O. Box 8, Kekaha, HI 96752 808-337-1413 

HI Richard McCormack 

and/or Alika Napier 

Pioneer Hi-Bred International Inc., 67-172 

Farrington Highway, P.O. Box 520, Waialua, HI 

96791 

808-637-0100 

IA Joseph Gogerty 

and/or Marcus Marine 

Pioneer Hi-Bred International Inc., 1920 E 

McGregor Road, P.O. Box 557, Algona, IA 50511 515-295-7283 

IA Delos Borcherding 

and/or Kerry Mounsey 

Pioneer Hi-Bred International Inc., 810 Sugar Grove 

Avenue, Dallas Center, IA 50063 515-270-4259 

IA Kurt Hohnstrater 

and/or Bob Bonewitz 

Pioneer Hi-Bred International Inc., 7300 NW 62nd 

Avenue, P.O. Box 1004, Johnston, IA 50131 515-334-4575 

PHI-2008-139 

April 20, 2009Page 200 of 416
IA Jeff Schussler and/or 

Ryan Pape 

Pioneer Hi-Bred International Inc., 3261 N. 

Alburnett Road, P.O. Box 428, Marion, IA 52302 319-377-2870 

IL Don Westerhof and/or 

David Hobbs 

Pioneer Hi-Bred International Inc., 985 County 

Road 300 East, Ivesdale, IL 61851 271-564-2339 

IL Steve Dofing and/or 

Bruce Eidson 

Pioneer Hi-Bred International Inc., 21888 N 950th

Road, Adair, IL 61411 309-653-2401 

IL Terry Williams and/or 

Chris Saacke 

Pioneer Hi-Bred International Inc., 19741 Illinois 

Highway 26, Princeton, IL 61356 815-875-6523 

IL Larry Hageman and/or 

Scott Swanson 

Dupont Research Farm, 13239 Route 38, Rochelle, 

IL 61068 815-562-7570 

IL Eric Luce and/or 

Trenton Borth 

Pioneer Hi-Bred International Inc., 3025 East 8th 

Road, Uttica, IL 61373 815-667-5133 

IN Brian Bush and/or 

Kevin Kuhn 

Pioneer Hi-Bred International Inc.,3850 North 100 

East, Windfall, IN 46076 765-945-8217 

IN Dan Pohl and/or JB 

Crow 

Pioneer Hi-Bred International Inc., 1193 W 400 S, 

Princeton, IN 47670 812-385-2935 

KS Travis Lee and/or 

Randy Sutterfield 

Pioneer Hi-Bred International Inc., 150 Air Links 

Drive, Garden City, KS 67846 620-276-8149 

MI Dina Severns and/or 

Bart Hubbell 

Pioneer Hi-Bred International Inc., 1635 N. Wisner 

Road, Ithaca, MI 48847 989-875-2220 

MN Steve Hauf and/or 

Cody Nelson 

Pioneer Hi-Bred International Inc., 4050 30th

Avenue South, Moorehead, MN 56560 218-299-8610 

MN Doug Sublet and/or 

Brian Merten 

Pioneer Hi-Bred International Inc., 19456 State 

Highway 22, Mankato, MN 56001 507-625-3252 

MN Ryan Lee and/or Dan 

Kleven 

Pioneer Hi-Bred International Inc., 1740 45th Street 

SE, Willmar, MN 56201 320-235-2422 

MO Gary Henke and/or 

Chris Zwiener 

Pioneer Hi-Bred International Inc., Route 1 Box 

49A, Miami, MO 65344 660-595-2242 

NE Dennis Dolan and/or 

Gregory Halstead 

Pioneer Hi-Bred International Inc., 1501 Road P, 

York, NE 68467 402-362-6639 

PA Dave Whitaker and/or 

Rick Blum 

Pioneer Hi-Bred International Inc., 982 New Holland 

Road, New Holland, PA 17557 717-354-6044 

PR 

Alberio Molina and/or 

Lorimar FigueroaPerez 

Pioneer Hi-Bred Puerto Rico, Road. #3, KM. 154.9, 

Salinas, PR 00751 787-824-4440 

SD Tim Larson and/or 

Daniel Hancock 

Pioneer Hi-Bred International Inc., 214 West 2nd St. 

South, Brookings, SD 57006 605-692-5966 

TN Russ Henry and/or 

Gray Hooks 

Pioneer Hi-Bred International Inc., 3092 Flippen 

Lane, Union City, TN 38261 731-885-4882 

TX Sharon Rogers and/or 

Jorge Guzman 

Pioneer Hi-Bred International, Inc., 1702 South 

International Blvd., Weslaco, TX 78596 956-969-1569 

PHI-2008-139 

April 20, 2009Page 201 of 416
WA Brett Iverson and/or 

Cindy Rochleau 

Pioneer Hi-Bred International Inc., 1040 Settler 

Road, Connell, WA 99326 509-234-9046 

WI Mike O’Leary and/or 

John Kinnard 

Pioneer Hi-Bred International Inc., S5380 Cemetery 

Road, Eau Claire, WI 54701 715-834-0061 

WI Tom Kevern and/or 

Ryan Temperly 

Pioneer Hi-Bred International Inc., 1039 South 

Milton-Shopiere, P.O. Box 668, Janesville, WI 

53547 

608-756-4030 

In addition to the above participants/states, the Participants listed below may have EUP Experiments 

with cooperators in any of the listed states and territories in the EUP Program. 

Participant Participant’s Address Phone 

Number 

Tim Nowatzki Pioneer Hi-Bred International Inc., 7300 NW 62nd Avenue, P.O. Box 

1004, Johnston, IA 50131 515-270-4270 

Mel Peters Pioneer Hi-Bred International Inc., 7300 NW 62nd Avenue, P.O. Box 

1004, Johnston, IA 50131 515-253-2156 

Lee Prochaska Pioneer Hi-Bred International Inc., 7250 NW 62nd Avenue, P.O. Box 

552, Johnston, IA 50131 515-334-6703 

Paula Davis Pioneer Hi-Bred International Inc., 7100 NW 62nd Avenue, P.O. Box 

1000, Johnston, IA 50131 515-334-6994 

Jeff Mathesius Pioneer Hi-Bred International Inc., 7100 NW 70th Avenue, P.O. Box 

183, Johnston, IA 50131 515-334-3603 

Mike Elsing Pioneer Hi-Bred International Inc., 7300 NW 62nd Avenue, P.O. Box 

1004, Johnston, IA 50131 515-253-2132 

Glen Umbaugh Pioneer Hi-Bred International Inc., 6900 NW 62nd Avenue, P.O. Box 

256, Johnston, IA 50131 515-270-3426 

Wayne Mehre Pioneer Hi-Bred International Inc., 7300 NW 62nd Avenue, P.O. Box 

1004, Johnston, IA 50131 515-253-2405 

Brian Baumhover Pioneer Hi-Bred International Inc., 7301 NW 62nd Avenue, P.O. Box 

85, Johnston, IA 50131 515-270-5902 

Doug Nubel Pioneer Hi-Bred International Inc., 7300 NW 62nd Avenue, P.O. Box 

1004, Johnston, IA 50131 515-253-5780 

Russ Fox Pioneer Hi-Bred International Inc., 810 Sugar Grove Avenue, Dallas 

Center, IA 500631 515-270-4088 

Carla Peterman Pioneer Hi-Bred International Inc., 7300 NW 62nd Avenue, P.O. Box 

1004, Johnston, IA 50131 515-270-5992 

Lindsey Flexner DuPont Experimental Station, Route 141, Henry Clay Bldg #353, 

Wilmington, DE 19880 302-695-6822 

Rachel Binning Pioneer Hi-Bred International Inc., 7250 NW 62nd Avenue, P.O. Box 

552, Johnston, IA 50131 515-254-2742 

Murt McLeod Pioneer Hi-Bred International Inc., 3850 North 100 East, Windfall, IN 

46076 765-945-8217 

PHI-2008-139 

April 20, 2009Page 202 of 416
G2. Acreage and Seed Quantities by State or Territory for the EUP Program 

Pioneer has endeavored to forecast its acreage needs and field testing program for 2009. The quantity of 

acres being requested in this application is required for product development and testing. As 

recommended in EPA’s “Tips for Plant-Incorporated Protectant (PIP) Experimental Use Permit (EUP) 

Program Submission” and in guidance provided in the February 6, 2009 letter from BPPD to Pioneer, this 

acreage calculation includes the following six categories: 

 Category 1: EUP Test Plants; 

 Category 2: Registered PIPs for breeding or comparison purposes; 

 Category 3: Non-registered PIPs; 

 Category 4: Registered PIPs (other than those listed in Category #2) for comparison purposes 

only (i.e., “commercial checks”); 

 Non-PIP plants (for example, herbicide tolerant or non-transgenic); and 

 Border or filler rows. 

Category 4 includes those commercial products that will be used for non-experimental purposes only 

(i.e., not for breeding)—for example, control checks in a yield evaluation. 

Table 6 lists the breeding stacks included in categories 1, 2 and 3. Each of the individual events listed 

is registered by EPA. All PIP proteins have full tolerance exemptions. 

Table 6. Breeding Stacks Included in Categories 1, 2 and 3 

Category 1 

EUP Test Plants 

Category 2 

Registered PIPs for breeding 

or comparison purposes 

Category 3 

Non-registered PIPs 

1507 x 59122 x MON810 1507 1507 x 59122 x MON810 x MIR162 

1507 x MON810 59122 1507 x 59122 x MON810 x MIR604 

59122 x MON810 MON810 1507 x 59122 x MIR162 x MIR604 

1507 x 59122 1507 x MON810 x MIR162 x MIR604 

MIR604 59122 x MON810 x MIR162 x MIR604 

Bt11 1507 x 59122 x MIR162 

Bt11 x MIR604 1507 x 59122 x MIR604 

MIR162 1507 x MON810 x MIR162 

Bt11 x MIR162 1507 x MON810 x MIR604 

Bt11 x MIR162 x MIR604 1507 x MIR162 x MIR604 

59122 x MON810 x MIR162 

591122 x MON810 x MIR604 

59122 x MIR162 x MIR604 

MON810 x MIR162 x MIR604 

1507 x MIR162 

1507 x MIR604 

 59122 x MIR162 

 59122 x MIR604 

 MON810 x MIR162 

 MON810 x MIR604 

 MIR162 x MIR604 

Many of the breeding stacks listed in Category 3 will likely not be planted to any appreciable extent. 

However, in order to enable full flexibility in the breeding and testing program, many potential 

combinations are listed. 

Table 7 provides the maximum acreage per state. This acreage will not be exceeded. 

PHI-2008-139 

April 20, 2009Page 203 of 416
The allocation of these acres between event combinations and protocols (Tables 8-13) represents 

best estimates as of this date and should not be used for inspection purposes. Environmental 

conditions, for example, weather events, pest infestation levels, unforeseen regulatory data demands 

received from other countries, and other factors, may necessitate a reallocation of acres between 

protocols and traits. For event combinations where less than an acre may be grown, “<1” has been 

entered in Tables 8-13 and is counted as zero acres for the purpose of estimating acres by event 

combinations and protocol (as per BPPD guidance). 

Table 14 summarizes the amount of seed to be planted and the total estimated amount of active 

ingredient to be used in the EUP Program. In this table, if the estimated acreage of an event 

combination is <1, we have assume that 1 acre will be grown (as per BPPD guidance). This has been 

done to estimate the maximum amount of a.i. that will be used per state. 

PHI-2008-139 

April 20, 2009Page 204 of 416
Table 7. Proposed Total Acreage by State

State PIP

PIPs for 

comparison 

only* Non-PIP Border Total

AR 37 4 6 27 74

CA 31 14 8 28 81

CO 20 5 4 18 47

DE 10 0 2 13 25

GA 41 6 6 24 77

HI 765 1 106 12 884

IA 99 31 24 99 253

IL 679 16 113 51 859

IN 55 14 11 42 122

KS 36 4 6 27 73

KY 7 0 14 12

LA 15 0 2 8 25

MI 18 3 3 13 37

MN 41 6 7 33 87

MO 32 5 6 27 70

MS 16 2 2 8 28

NC 15 0 2 8 25

NE 52 14 12 48 126

NY 7 0 17 15

OH 10 0 2 13 25

OK 15 0 2 6 23

PA 22 4 4 23 53

PR 56 1 6 12 75

SD 30 4 5 25 64

TN 29 5 5 26 65

TX 38 5 7 29 79

WA 660 0 100 0 760

WI 31 6 5 20 62

totals 2867 150 458 651 4126

* This acreage is for registered PIPs that are used for comparison purposes 

only and are not used for breeding.

Acres

PHI-2008-139 

April 20, 2009Page 205 of 416
Table 8. Estimated Acreage per State/Territory for Nursery / Breeding Protocol

*

State

1507x59122xMON810

1507xMON810

59122xMON810

Category 1 Total

1507

59122

MON810

1507x59122

MIR604

Bt11

Bt11xMIR604

MIR162

Bt11xMIR162

Bt11xMIR162xMIR604

Category 2 Total

1507x59122xMON810xMIR162

1507x59122xMON810xMIR604

1507x59122xMIR162xMIR604

1507xMON810xMIR162xMIR604

59122xMON810xMIR162xMIR604

1507x59122xMIR162

1507x59122xMIR604

1507xMON810xMIR162

1507xMON810xMIR604

1507xMIR162xMIR604

59122xMON810xMIR162

59122xMON810xMIR604

59122xMIR162xMIR604

MON810xMIR162xMIR604

1507xMIR162

1507xMIR604

59122xMIR162

59122xMIR604

MON810xMIR162

MON810xMIR604

MIR162xMIR604

Category 3 Total

Category 4**

Total PIP

Non-PIP

Border

Total NON-PIP

Total

GA 3 3 4 10 <1 <1 <1 2 <1 <1 <1 <1 <1 <1 3 < 1 < 1 < 1 < 1 < 1 < 1 < 1 < 1 < 1 < 1 < 1 < 1 < 1 < 1 <1 < 1 < 1 < 1 < 1 < 1 < 1 1 0 14 1 2 3 17

HI 10 10 10 30 4435111111 22 < 1 < 1 < 1 < 1 < 1 < 1 < 1 < 1 < 1 < 1 < 1 < 1 < 1 < 1 <1 < 1 < 1 < 1 < 1 < 1 < 1 1 0 53 5 9 14 67

PR 7 8 11 26 4435111111 22 < 1 < 1 < 1 < 1 < 1 < 1 < 1 < 1 < 1 < 1 < 1 < 1 < 1 < 1 <1 < 1 < 1 < 1 < 1 < 1 < 1 1 0 49 5 9 14 63

total 66 47 3 0 116 31 147

* The allocation of these acres among various event combinations represents best estimates as of this date and should not be used for inspection purposes. Environmental conditions (** Category 4 is non-experimental material that will be used for check plots only (not breeding). 

e.g. weather events, pest infestation levels), unforeseen regulatory data 

demands received from other countries, and other factors, may necessitate a reallocation of acres between protocols and traits. For event combinations where less than an acre may be grown, “<1” has been entered and the cumlative total of the "<1" acres is

counted as one acre for the purpose of estimating acres within a Category across event combinations and protocol (as per BPPD guidance).

Category 5 Category 3 Category 1 Category 2

PHI-2008-139 

April 20, 2009Page 206 of 416
Table 9. Estimated Acreage per State/Territory for Yield / Agronomic Protocol*

State

1507x59122xMON810

1507xMON810

59122xMON810

Category 1 Total

1507

59122

MON810

1507x59122

MIR604

Bt11

Bt11xMIR604

MIR162

Bt11xMIR162

Bt11xMIR162xMIR604

Category 2 Total

1507x59122xMON810xMIR162

1507x59122xMON810xMIR604

1507x59122xMIR162xMIR604

1507xMON810xMIR162xMIR604

59122xMON810xMIR162xMIR604

1507x59122xMIR162

1507x59122xMIR604

1507xMON810xMIR162

1507xMON810xMIR604

1507xMIR162xMIR604

59122xMON810xMIR162

59122xMON810xMIR604

59122xMIR162xMIR604

MON810xMIR162xMIR604

1507xMIR162

1507xMIR604

59122xMIR162

59122xMIR604

MON810xMIR162

MON810xMIR604

MIR162xMIR604

Category 3 Total

Category 4**

Total PIP

Non-PIP

Border

Total NON-PIP

Total

AR 3 1 1 5 1 <1 <1 1 <1 <1 <1 <1 <1 <1 3 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 2 3 13 2 8 10 23CA 11 3 1 15 2 2 1 8 <1 <1 <1 <1 <1 <1 14 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 2 14 45 8 28 36 81

CO 4 1 1 6 1 1 <1 2 <1 <1 <1 <1 <1 <1 5 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 2 5 18 3 11 14 32

GA 4 1 1 6 1 1 <1 2 <1 <1 <1 <1 <1 <1 5 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 2 5 18 3 12 15 33

IA 22 8 1 31 10 5 2 12 <1 <1 <1 <1 <1 <1 30 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 2 30 93 17 63 80 173

IL 11 4 1 16 3 3 <1 8 <1 <1 <1 <1 <1 <1 15 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 2 15 48 8 29 37 85

IN 11 2 1 14 2 2 <1 9 <1 <1 <1 <1 <1 <1 14 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 2 13 43 7 24 31 74

KS 3 1 1 5 1 <1 <1 2 <1 <1 <1 <1 <1 <1 4 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 2 3 14 2 9 11 25

MI 3 1 1 5 1 <1 <1 2 <1 <1 <1 <1 <1 <1 4 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 2 3 14 2 9 11 25

MN 4 1 1 6 1 <1 <1 4 <1 <1 <1 <1 <1 <1 6 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 2 5 19 3 12 15 34

MO 4 1 1 6 1 <1 <1 4 <1 <1 <1 <1 <1 <1 6 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 2 5 19 3 12 15 34

MS 2 1 1 4 <1 <1 <1 2 <1 <1 <1 <1 <1 <1 3 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 2 2 11 1 4 5 16

NE 12 2 1 15 2 2 1 9 <1 <1 <1 <1 <1 <1 15 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 2 14 46 8 28 36 82

PA 3 1 1 5 1 <1 <1 3 <1 <1 <1 <1 <1 <1 5 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 2 4 16 2 10 12 28

SD 3 1 1 5 1 <1 <1 3 <1 <1 <1 <1 <1 <1 5 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 2 4 16 2 10 12 28

TN 3 1 1 5 1 <1 <1 3 <1 <1 <1 <1 <1 <1 5 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 2 4 16 2 10 12 28

TX 3 3 1 7 1 1 1 3 <1 <1 <1 <1 <1 <1 7 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 2 4 20 3 13 16 36

WI 511 7 1 1 <1 4 <1 <1 <1 <1 <1 <1 7 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 2 6 22 3 13 16 38

total 163 153 36 139 491 384 875

* The allocation of these acres among various event combinations represents best estimates as of this date and should not be used for inspection purposes. Environmental conditions (** Category 4 is non-experimental material that will be used for check plots only (not breeding). 

e.g . weather events, pest infestation levels), unforeseen regulatory data 

demands received from other countries, and other factors, may necessitate a reallocation of acres between protocols and traits. For event combinations where less than an acre may be grown, “<1” has been entered and the cumlative total of the "<1" acres is counted as one acre for the purpose of estimating acres within a Category across event combinations and protocol (as per BPPD guidance).

Category 5 Category 3 Category 1 Category 2

PHI-2008-139 

April 20, 2009Page 207 of 416
Table 10. Estimated Acreage per State/Territory for Efficacy / IRM Protocol*

State

1507x59122xMON810

1507xMON810

59122xMON810

Category 1 Total

1507

59122

MON810

1507x59122

MIR604

Bt11

Bt11xMIR604

MIR162

Bt11xMIR162

Bt11xMIR162xMIR604

Category 2 Total

1507x59122xMON810xMIR162

1507x59122xMON810xMIR604

1507x59122xMIR162xMIR604

1507xMON810xMIR162xMIR604

59122xMON810xMIR162xMIR604

1507x59122xMIR162

1507x59122xMIR604

1507xMON810xMIR162

1507xMON810xMIR604

1507xMIR162xMIR604

59122xMON810xMIR162

59122xMON810xMIR604

59122xMIR162xMIR604

MON810xMIR162xMIR604

1507xMIR162

1507xMIR604

59122xMIR162

59122xMIR604

MON810xMIR162

MON810xMIR604

MIR162xMIR604

Category 3 Total

Category 4**

Total PIP

Non-PIP

Border

Total NON-PIP

Total

AR 2 1 1 4 <1 <1 <1 2 <1 <1 <1 <1 <1 <1 3 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 119 1 4 5 14

GA 1 1 1 3 <1 <1 <1 2 <1 <1 <1 <1 <1 <1 3 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 118 1 3 4 12

HI 1 1 1 3 <1 <1 <1 2 <1 <1 <1 <1 <1 <1 3 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 118 1 3 4 12

IA 2 1 1 4 <1 <1 <1 2 <1 <1 <1 <1 <1 <1 3 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 119 1 4 5 14

IL 1 1 1 3 <1 <1 <1 2 <1 <1 <1 <1 <1 <1 3 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 118 1 3 4 12

IN 1 1 1 3 <1 <1 <1 2 <1 <1 <1 <1 <1 <1 3 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 118 1 3 4 12

KS 1 1 1 3 <1 <1 <1 2 <1 <1 <1 <1 <1 <1 3 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 118 1 3 4 12

MN 1 1 1 3 <1 <1 <1 2 <1 <1 <1 <1 <1 <1 3 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 118 1 3 4 12

PR 1 1 1 3 <1 <1 <1 2 <1 <1 <1 <1 <1 <1 3 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 118 1 3 4 12

TN 1 1 1 3 <1 <1 <1 2 <1 <1 <1 <1 <1 <1 3 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 118 1 3 4 12

TX 111 3 <1 <1 <1 2 <1 <1 <1 <1 <1 <1 3 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 118 1 3 4 12

total 35 33 11 11 90 46 136* The allocation of these acres among various event combinations represents best estimates as of this date and should not be used for inspection purposes. Environmental conditions (** Category 4 is non-experimental material that will be used for check plots only (not breeding). 

e.g. weather events, pest infestation levels), unforeseen regulatory data 

demands received from other countries, and other factors, may necessitate a reallocation of acres between protocols and traits. For event combinations where less than an acre may be grown, “<1” has been entered and the cumlative total of the "<1" acres is counted as one acre for the purpose of estimating acres within a Category across event combinations and protocol (as per BPPD guidance).

Category 3 Category 5 Category 1 Category 2

PHI-2008-139 

April 20, 2009Page 208 of 416
Table 11. Estimated Acreage per State/Territory for Efficacy / IRM with No Isolation Protocol*

State

1507x59122xMON810

1507xMON810

59122xMON810

Category 1 Total 

1507

59122

MON810

1507x59122

MIR604

Bt11

Bt11xMIR604

MIR162

Bt11xMIR162

Bt11xMIR162xMIR604

Category 2 Total

Category 3 

Category 4**

Total PIP

Non-PIP

Border

Total NON-PIP

Total

AR 411 6 1 <1 <1 3 <1 <1 <1 <1 <1 <1 5 0 0 11 2 13 15 26

CO 211 4 <1 <1 <1 2 <1 <1 <1 <1 <1 <1 3007 1 7 8 15

DE 311 5 1 <1 <1 3 <1 <1 <1 <1 <1 <1 5 0 0 10 2 13 15 25

GA 211 4 <1 <1 <1 2 <1 <1 <1 <1 <1 <1 3007 1 7 8 15

IA 821 11 2 <1 <1 6 <1 <1 <1 <1 <1 <1 9 0 0 20 5 30 35 55

IL 411 6 1 <1 <1 3 <1 <1 <1 <1 <1 <1 5 0 0 11 3 17 20 31

IN 311 5 1 <1 <1 3 <1 <1 <1 <1 <1 <1 5 0 0 10 2 13 15 25

KS 311 5 1 <1 <1 3 <1 <1 <1 <1 <1 <1 5 0 0 10 2 13 15 25

KY 211 4 <1 <1 <1 2 <1 <1 <1 <1 <1 <1 3007 1 4 5 12

LA 211 4 <1 <1 <1 2 <1 <1 <1 <1 <1 <1 3007 1 6 7 14

MI 211 4 <1 <1 <1 2 <1 <1 <1 <1 <1 <1 3007 1 4 5 12

MN 411 6 1 <1 <1 4 <1 <1 <1 <1 <1 <1 6 0 0 12 2 16 18 30

MO 311 5 1 <1 <1 3 <1 <1 <1 <1 <1 <1 5 0 0 10 2 13 15 25

MS 211 4 <1 <1 <1 2 <1 <1 <1 <1 <1 <1 3007 1 4 5 12

NC 211 4 <1 <1 <1 2 <1 <1 <1 <1 <1 <1 3007 1 6 7 14

NE 411 6 1 <1 <1 4 <1 <1 <1 <1 <1 <1 6 0 0 12 3 18 21 33

NY 211 4 <1 <1 <1 2 <1 <1 <1 <1 <1 <1 3007 1 7 8 15

OH 311 5 1 <1 <1 3 <1 <1 <1 <1 <1 <1 5 0 0 10 2 13 15 25

OK 211 4 <1 <1 <1 2 <1 <1 <1 <1 <1 <1 3007 1 4 5 12

PA 311 5 1 <1 <1 3 <1 <1 <1 <1 <1 <1 5 0 0 10 2 13 15 25

SD 311 5 1 <1 <1 3 <1 <1 <1 <1 <1 <1 5 0 0 10 2 13 15 25

TN 311 5 1 <1 <1 3 <1 <1 <1 <1 <1 <1 5 0 0 10 2 13 15 25

TX 211 4 <1 <1 <1 2 <1 <1 <1 <1 <1 <1 3007 2 11 13 20

WI 211 4 <1 <1 <1 2 <1 <1 <1 <1 <1 <1 3007 1 5 6 13

total 119 104 0 0 223 306 529

** Category 4 is non-experimental material that will be used for check plots only (not breeding). 

Category 5

* The allocation of these acres among various event combinations represents best estimates as of this date and should not be used for inspection purposes. Environmental conditions (e.g. weather events, pest infestation levels), unforeseen regulatory data demands received from other countries, and other factors, may necessitate a reallocation of acres between protocols and traits. For event combinations where less than an acre may be grown, “<1” has been entered and the cumlative total of the "<1" acres is counted as one acre for the purpose of estimating acres within a Category across event combinations and protocol (as per BPPD guidance).

Category 1 Category 2

PHI-2008-139 

April 20, 2009Page 209 of 416
Table 12. Estimated Acreage per State/Territory for Seed Production Protocol*

State

1507x59122xMON810

1507xMON810

59122xMON810

Category 1 Total

1507

59122

MON810

1507x59122

MIR604

Bt11

Bt11xMIR604

MIR162

Bt11xMIR162

Bt11xMIR162xMIR604

Category 2 Total

Category 3

Category 4**

Total PIP

Non-PIP

Border

Total Non-PIP

Total

HI 40 40 325 405 100 60 60 50 5 5 5 5 5 5 300 0 0 705 100 0 100 805

IL 10 10 300 320 100 60 60 50 5 5 5 5 5 5 300 0 0 620 100 0 100 720

WA 20 20 320 360 100 60 60 50 5 5 5 5 5 5 300 0 0 660 100 0 100 760

total 1085 900 0 0 1985 300 2285

** Category 4 is non-experimental material that will be used for check plots only (not breeding). 

Category 5

* The allocation of these acres among various event combinations represents best estimates as of this date and should not be used for inspection purposes. Environmental conditions (e.g. weather events, pest infestation levels) unforeseen regulatory data demands received from other countries, and other factors, may 

necessitate a reallocation of acres between protocols and traits. 

Category 2 Category 1

PHI-2008-139 

April 20, 2009Page 210 of 416
Table 13. Estimated Acreage per State/Territory for Regulatory Protocol*

State

1507x59122xMON810

1507xMON810

59122xMON810

Category 1 Total

1507

59122

MON810

1507x59122

MIR604

Bt11

Bt11xMIR604

MIR162

Bt11xMIR162

Bt11xMIR162xMIR604

Category 2 Total

1507x59122xMON810xMIR162

1507x59122xMON810xMIR604

1507x59122xMIR162xMIR604

1507xMON810xMIR162xMIR604

59122xMON810xMIR162xMIR604

1507x59122xMIR162

1507x59122xMIR604

1507xMON810xMIR162

1507xMON810xMIR604

1507xMIR162xMIR604

59122xMON810xMIR162

59122xMON810xMIR604

59122xMIR162xMIR604

MON810xMIR162xMIR604

1507xMIR162

1507xMIR604

59122xMIR162

59122xMIR604

MON810xMIR162

MON810xMIR604

MIR162xMIR604

Category 3 Total

Category 4**

Total PIP

Non-PIP

Border

Total NON-PIP

Total

AR 2 2 1 5 <1 <1 <1 1 <1 <1 <1 <1 <1 <1 2 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 108 1 2 3 11

IA 2 2 1 5 <1 <1 <1 1 <1 <1 <1 <1 <1 <1 2 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 108 1 2 3 11

IL 2 2 1 5 <1 <1 <1 1 <1 <1 <1 <1 <1 <1 2 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 108 1 2 3 11

IN 2 2 1 5 <1 <1 <1 1 <1 <1 <1 <1 <1 <1 2 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 108 1 2 3 11

KS 2 2 1 5 <1 <1 <1 1 <1 <1 <1 <1 <1 <1 2 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 108 1 2 3 11

LA 2 2 1 5 <1 <1 <1 1 <1 <1 <1 <1 <1 <1 2 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 108 1 2 3 11

MN 2 2 1 5 <1 <1 <1 1 <1 <1 <1 <1 <1 <1 2 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 108 1 2 3 11

MO 2 2 1 5 <1 <1 <1 1 <1 <1 <1 <1 <1 <1 2 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 108 1 2 3 11

NC 2 2 1 5 <1 <1 <1 1 <1 <1 <1 <1 <1 <1 2 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 108 1 2 3 11

NE 2 2 1 5 <1 <1 <1 1 <1 <1 <1 <1 <1 <1 2 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 108 1 2 3 11

OK 2 2 1 5 <1 <1 <1 1 <1 <1 <1 <1 <1 <1 2 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 108 1 2 3 11

SD 2 2 1 5 <1 <1 <1 1 <1 <1 <1 <1 <1 <1 2 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 108 1 2 3 11

TX 2 2 1 5 <1 <1 <1 1 <1 <1 <1 <1 <1 <1 2 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 108 1 2 3 11

WI 221 5 <1 <1 <1 1 <1 <1 <1 <1 <1 <1 2 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 108 1 2 3 11

total 70 28 14 0 112 42 154

* The allocation of these acres among various event combinations represents best estimates as of this date and should not be used for inspection purposes. Environmental conditions (** Category 4 is non-experimental material that will be used for check plots only (not breeding). 

e.g. weather events, pest infestation levels), unforeseen regulatory data 

demands received from other countries, and other factors, may necessitate a reallocation of acres between protocols and traits. For event combinations where less than an acre may be grown, “<1” has been entered and the cumlative total of the "<1" acres is counted as one acre for the purpose of estimating acres within a Category across event combinations and protocol (as per BPPD guidance).

Category 5 Category 1 Category 2 Category 3

PHI-2008-139 

April 20, 2009Page 211 of 416
Table 14. Summary of EUP Program Quantities for 2009

State Category 1 Category 2 Category 3

Cry1F 

(gm)

Cry34/35Ab1 

(gm)

Cry1Ab 

(MON810)

(gm)

VIP3

 (gm)

Cry3a 

(gm)

Cry1Ab 

(Bt11)

(gm)

AR 168.4 109.4 33.7 0.80 10.16 0.08 4.44 0.14 0.22

CA 126.3 117.8 16.8 0.66 8.47 0.07 4.18 0.13 0.24

CO 84.2 67.3 16.8 0.43 5.47 0.04 2.61 0.08 0.13

DE 42.1 42.1 0.0 0.21 2.74 0.02 1.30 0.04 0.08

GA 193.6 117.8 33.7 0.89 11.28 0.09 4.70 0.15 0.24

HI 3686.9 2735.7 16.8 16.56 210.68 1.70 85.33 2.75 5.47

IA 429.3 370.4 33.7 2.13 27.12 0.22 12.53 0.40 0.74

IL 2946.1 2735.7 33.7 14.60 186.02 1.51 85.85 2.77 5.47

IN 227.3 202.0 33.7 1.18 15.03 0.12 7.31 0.24 0.40

KS 151.5 117.8 33.7 0.77 9.85 0.08 4.70 0.15 0.24

KY 33.7 25.3 0.0 0.15 1.93 0.02 0.78 0.03 0.05

LA 75.8 42.1 8.4 0.33 4.14 0.03 1.57 0.05 0.08

MI 75.8 58.9 16.8 0.39 4.92 0.04 2.35 0.08 0.12

MN 168.4 143.1 33.7 0.88 11.20 0.09 5.48 0.18 0.29

MO 134.7 109.4 25.3 0.69 8.75 0.07 4.18 0.13 0.22

MS 67.3 50.5 16.8 0.34 4.38 0.04 2.09 0.07 0.10

NC 75.8 42.1 8.4 0.33 4.14 0.03 1.57 0.05 0.08

NE 218.9 193.6 25.3 1.12 14.23 0.12 6.78 0.22 0.39

NY 33.7 25.3 0.0 0.15 1.93 0.02 0.78 0.03 0.05

OH 42.1 42.1 0.0 0.21 2.74 0.02 1.30 0.04 0.08

OK 75.8 42.1 8.4 0.33 4.14 0.03 1.57 0.05 0.08

PA 84.2 84.2 16.8 0.47 5.99 0.05 3.13 0.10 0.17

PR 244.1 210.4 16.8 1.20 15.35 0.12 7.05 0.23 0.42

SD 126.3 101.0 25.3 0.64 8.21 0.07 3.91 0.13 0.20

TN 109.4 109.4 25.3 0.62 7.90 0.06 4.18 0.13 0.22

TX 159.9 126.3 33.7 0.82 10.40 0.08 4.96 0.16 0.25

WA 3030.3 2525.3 0.0 14.24 181.31 1.47 78.28 2.53 5.05

WI 134.7 101.0 25.3 0.67 8.49 0.07 3.91 0.13 0.20

total 12946 10648 539 62 787 6 347 11 21

Assumptions: 

 3) The g/kg of seed used for 1507 (0.0024 g/kg Cry1F), 59122 (0.031g/kg Cry34Ab1) and MON810 (0.00027 g/kg Cry1Ab) were based on the values observed

 in PHI-2008-006 (MRID 475109-04). The amount of a.i for MIR162 (0.031 g/kg VIP3A), MIR604 (0.001 g/kg mCry3A) and Bt11 (0.002 g/kg Cry1Ab) were based 

on the levels presented in Syngenta EUP application for MIR162.

kgs of seed per Category

 1) Assumed 100% of the EUP test plants (Category 1) were 1507x59122xMON810 (0.0027g/kg Cry1F, 0.034 g/kg Cry34/35Ab1 and 0.00026g/kg 

Cry1Ab). Values based on expression levels observed in PHI-2008-006 (MRID 475109-04).

 2) Assumed 100% of the acres in Category 2 and Category 3 were planted to each registered single event (i.e. 1507, 59122, MON810, MIR162 and MIR604)

except for Bt11 (Category 2 only). 

Total A.I. Quantities

PHI-2008-139 

April 20, 2009Page 212 of 416
G3. Details of the Proposed EUP Program 

Crop: Corn (Zea mays L.)

Target Pests: 

Agrotis ipsilon (Black cutworm) 

Helicoverpa zea (Corn earworm or cotton bollworm) 

Ostrinia nubilalis (European corn borer) 

Spodoptera frugiperda (Fall armyworm) 

Diatraea grandiosella (Southwestern corn borer) 

Richia albicosta (Western bean cutworm) 

Elasmopalpus lignosellus (Lesser corn stalk borer) 

Diatrae crambidoides (Southern corn stalk borer) 

Diatrea saccharalis (Sugarcane borer) 

Papaipema nebris (Common stalk borer) 

Diabrotica virgifera virgifera (western corn rootworm) 

Diabrotica barberi (northern corn rootworm) 

Diagrotica virgifera zeae (Mexican corn rootworm) 

Major Geographic Areas: 27 states and Puerto Rico, representing diverse agronomic zones

Sites: Pioneer research centers, seed production fields, GLP cooperator fields, privately owned farms 

and university farms and testing sites. EUP Blocks may or may not be located at EPA registered 

pesticide-producing establishments. All EUP Blocks will be planted on existing agricultural land.

Period of Use: April 1, 2009 through March 31, 2010.

Protocols for Field Experimentation: 

Research planned under the EUP Program will be conducted under the following six protocols: 

1. Nursery / Breeding; 

2. Yield / Agronomic; 

3. Efficacy / IRM; 

4. Efficacy / IRM with No Isolation; 

5. Seed Production; and 

6. Regulatory. 

G4. Definitions 

EUP Test Plants – Plants that contain 1507x59122xMON810, 1507xMON810 or 

59122xMON810. 

Experiment -- An Experiment is the unit of measure used to coordinate and track specific field 

activities. Experiment ID’s are assigned to each Experiment. 

EUP Experiment - Experiments containing EUP Test Plants are defined as EUP Experiments. 

EUP Experiments may also contain registered PIPs for breeding or comparison (if listed on the 

Master Label), non-registered PIPs (if listed on the Master Label), registered PIPs for comparison 

only (i.e., “commercial checks”), and/or non-PIPs (such as herbicide-tolerant or non-transgenic 

corn). 

PHI-2008-139 

April 20, 2009Page 213 of 416
EUP Block - The EUP Block is defined as an area of land in which EUP Experiments are planted 

(including filler corn, if planted between EUP Experiments). An EUP Block may consist of one, several 

or many EUP Experiments. In cases where the EUP Block is planted directly adjacent to non-EUP 

plants, the EUP Block will be separated from non-EUP plants by an alleyway (approximately 10 feet 

wide) or by a minimum 4 rows (approximately 10 feet wide) of purple-colored corn. The alleyway or 

purple-colored corn serves as a means of separating the EUP Block from the non-EUP corn. See 

Figures 1 and 2 for examples of EUP Block configurations. The alleyway or purple-colored corn is not 

included in the acreage of the EUP Block. 

G5. Universal Protocol Procedures 

The following procedures will be used for all EUP Experiments. 

Shipping and Labeling 

 Each shipment containing EUP seed must contain a copy of the EUP Master Label. A Master 

Label Weight Summary may be used to summarize the amount of seed in the shipment, so long 

as it accompanies the EUP Master Label. See Figure 3 for an example of a Master Label Weight 

Summary. 

 Seed will be shipped within two layers of packaging material consisting of a primary container 

within a secondary container, each of which must be independently capable of preventing the loss 

of seed. 

 Packaging from seed shipments is typically destroyed after shipments have been received. 

 Documentation will be kept of seed received, planted, harvested, shipped and destroyed. 

Schedule 

 For the continental U.S., planting of trials generally takes place from early March through the 

month of June. Observations and harvesting generally occur after planting through August to 

November. For Hawaii and Puerto Rico, there may be multiple plantings that occur throughout the 

year. 

Planting 

 Seed may be planted by hand or machine. 

o Do not re-use seed packets. 

o Dispose of any remaining seed according to accepted disposition methods (see “Final 

Disposition of EUP Material” in each protocol). 

 In cases where the EUP Block is planted directly adjacent to non-EUP plants, the EUP Block will 

be separated from non-EUP plants by an alleyway (approximately 10 ft wide) or by a minimum 4 

rows (approximately 10 feet wide) of purple-colored corn. The alleyway or purple-colored corn 

serves as a means of separating the EUP Block from the non-EUP corn. 

 After planting, planters will be cleaned while in the EUP Block. 

 Border rows may be used for agronomic purposes, but are not required under this EUP Program. 

Post-Trial Monitoring 

 EUP Blocks will be managed for volunteer plants to prevent persistence of EUP Test Plants in 

the environment. Any volunteer plants found will be destroyed using an appropriate herbicide or 

by mechanical means before reaching reproductive stage. 

 Upon full federal and state EPA approval of the EUP Test Plants, all post-trial monitoring will 

cease immediately. 

PHI-2008-139 

April 20, 2009Page 214 of 416
Storing EUP Material 

 EUP Test Plant seed will be stored in a secure facility with a sign stating that regulated, 

genetically modified material is stored inside. 

 EUP Test Plant seed will be labeled to enable ready identification of materials. Examples of 

labeling include (but are not limited to) color coding, marking, tagging, or otherwise distinguishing 

seed to allow immediate recognition. 

G6. Special Protocol Exemptions 

Efficacy / IRM with No Isolation 

Pioneer is requesting that the Efficacy / IRM Protocol with No Isolation not be required to have 660 

feet. isolation from other corn, including commercial corn. The reasons for this are several-fold: 

1. This protocol contains only EUP Test Plants, Registered PIPs, and Non-PIP corn. Each 

individual event is registered, and all PIP proteins have full tolerance exemptions. 

2. Under this protocol, there will be no non-registered PIP plants (Category 3) (see Table 12). 

3. The single events have been fully evaluated for potential environmental effects. Data 

demonstrate that 1507 and MON810 are additive (not synergistic or antagonistic) against 

several key pests (European corn borer, southwestern corn borer, corn earworm and fall 

armyworm). Monarch larvae studies demonstrate that there are no adverse effects of the 

EUP Test Plants. Expression levels (as measured by quantitative ELISA) in various tissues of 

the EUP Test Plants are similar to the individual events that comprise them. Therefore, we do 

not anticipate any adverse environmental impact. 

4. We will be conducting experiments with key academic stakeholders. Because many university 

farms/locations do not have isolated locations, requiring isolation would prohibit most 

academics from participating in the Efficacy / IRM program. 

5. We will be screening for efficacy against secondary pests that can’t be reared in culture (e.g.

western bean cutworm). The best data come from corn fields with historical insect pressure, 

and often these locations do not have 660 ft. isolation from commercial corn. 

6. All grain and vegetative material produced under the Efficacy / IRM Protocol with No Isolation 

will be destroyed. Grain will not enter the food/feed stream. 

7. The proposed acreage under this protocol is relatively low (less than 84 acres of EUP Test 

Plants). In addition, the proposed EUP program is for a relatively short duration (one growing 

season). Therefore, environmental exposure to the EUP Test Plants will be minimal. 

Seed Production 

Pioneer is requesting the option of using unwanted seed and vegetative material generated under the 

Seed Production Protocol for local animal feed and bedding. The reasons for this are several-fold: 

1. This protocol contains only EUP Test Plants, Registered PIPs, and Non-PIP corn. Each 

individual event is registered, and all PIP proteins have full tolerance exemptions. 

2. Under this protocol, there will be no non-registered PIP plants (Category 3) (see Table 13). 

3. This option is consistent with typical disposal practices at some Pioneer inbred production 

facilities. 

4. Expression levels (as measured by quantitative ELISA) in various tissues of the EUP Test 

Plants are similar to the individual events that comprise them. Therefore, we do not anticipate 

increased exposure. 

5. The proposed EUP program is for a relatively short duration (12 months). 

PHI-2008-139 

April 20, 2009Page 215 of 416


Figure 3. Example of Master Label Weight Summary 

Master Label Weight Summary 

29964-EUPEvent Weight (lbs) 

1507xMON810 0.1 

1507x59122xMON810 0.1 

1507 0.05 

MON810 0.05 

Bt11xMIR162 0.03 

Bt11 0.03 

MIR162 .02 

59122 .02 

MIR604 .01 

PHI-2008-139 

April 20, 2009Page 218 of 416
Nursery / Breeding Protocol 

General Objectives 

 Introgress events 1507, 59122 and MON810 into elite inbred corn lines. 

 Produce research hybrid seed for evaluation in field, greenhouse and/or laboratory trials.

 Collect phenotypic data on converted inbred lines. 

 Collect plant tissue on converted inbred lines for laboratory analysis. 

Description 

Inbreds may be hand pollinated (selfed, backcrossed or crossed to tester lines) to produce new inbred 

and hybrids for evaluation. Some inbreds may be open pollinated in isolation to produce seed for 

phenotypic analysis. Plants may be treated with herbicides and/or insecticides. Seed is generally 

retained for future breeding or testing purposes. 

Gene Containment 

One or more of the following practices will be used: 

1. Spatial isolation 

Option A. The EUP Block will be separated from any sexually compatible corn by at least 660 

feet. 

Option B. Corn can be grown within 660 feet of an EUP Block. All open-pollinated grain within 

660 feet of the EUP Block will be disposed as stated in “Final Disposition of the EUP Material.” 

Option C. Hand pollinations of non-EUP plants can occur within 660 feet of an EUP Block. Seed 

resulting from such hand-pollinations may be retained for future breeding and product 

development purposes if: 

 1) The hand-pollinated corn plants are pollinated with pollen from non-EUP plants; and 

 2) The ear shoots of the hand-pollinated corn plants are covered for the period of time during 

which the silks of the plants are sexually receptive. 

Remaining open-pollinated grain within 660 feet of the EUP Block will be disposed as stated in 

“Final Disposition of the EUP Material.” 

2. Temporal isolation 

The flowering period for plants within the EUP Block will not coincide with the presence of sexually 

receptive corn within 660 feet. 

3. Reproductive isolation 

The EUP Test Plants and non-registered PIP plants will be detasseled or destroyed prior to the 

onset of anthesis. 

PHI-2008-139 

April 20, 2009Page 219 of 416
Harvest Procedures 

 Hand or machine harvest. 

 Clean combine or picker in the regulated transgenic site. 

 Store retained seed in a clearly labeled and secure facility. 

 After harvest, cultivate remaining vegetative material into the regulated transgenic site. 

Final Disposition of EUP Material 

Final disposition of EUP seed or grain not saved for further breeding or testing purposes will be 

accomplished using one of the methods below. 

o cultivation into soil at the regulated transgenic site; 

o incineration; 

o burial (at least two feet deep); 

o composting; 

o autoclaving; 

o disposal at an approved landfill. 

Upon full federal and state EPA approval of the EUP Test Plants, all disposition restrictions will 

cease immediately. 

PHI-2008-139 

April 20, 2009Page 220 of 416
Yield / Agronomic Protocol 

General Objectives 

 Assess the agronomic impact of combining the 1507, 59122 and MON810 events in various 

hybrid backgrounds under various environmental and insect pressure conditions. 

 Assess the yield and agronomic properties of EUP test inbreds and hybrids compared to other 

single and stacked event inbreds and hybrids (both registered and non-registered PIP single 

events and breeding stacks), commercial check hybrids (registered PIPs), conventional 

hybrids, and/or insecticide treatments. 

 Assess the herbicide tolerance of EUP Test Plants (including EUP Test Plants that have been 

crossed with non-PIP herbicide tolerant lines of corn). 

Description 

EUP Blocks will generally be planted in multiple locations using randomized block design, strips or 

single large blocks depending on study type. Seed may be treated with a variety of insecticidal seed 

treatments or blended with non-transgenic or transgenic corn to evaluate agronomic performance 

including yield. Plants may be treated with herbicide and/or insecticides. Commercial checks may be 

planted as comparators. Harvested grain is generally not retained for breeding purposes. 

Gene Containment 

One or more of the following practices will be used: 

1. Spatial isolation 

Option A. The EUP Block will be separated from any sexually compatible corn by at least 660 

feet. 

Option B. Corn can be grown within 660 feet of an EUP Block. All open-pollinated grain within 

660 feet of the EUP Block will be disposed as stated in “Final Disposition of the EUP Material.” 

Option C. Hand pollinations of non-EUP plants can occur within 660 feet of an EUP Block. Seed 

resulting from such hand-pollinations may be retained for future breeding and product 

development purposes if: 

 1) The hand-pollinated corn plants are pollinated with pollen from non-EUP plants; and 

 2) The ear shoots of the hand-pollinated corn plants are covered for the period of time during 

which the silks of the plants are sexually receptive. 

Remaining open-pollinated grain within 660 feet of the EUP Block will be disposed as stated in 

“Final Disposition of the EUP Material.” 

2. Temporal isolation 

The flowering period for plants within the EUP Block will not coincide with the presence of sexually 

receptive corn within 660 feet. 

3. Reproductive isolation 

The EUP Test Plants and non-registered PIP plants will be detasseled or destroyed prior to the 

onset of anthesis. 

PHI-2008-139 

April 20, 2009Page 221 of 416
Harvest Procedures 

 Hand or machine harvest. 

 Clean combine or picker in the regulated transgenic site. 

 Store retained seed in a clearly labeled and secure facility. 

 After harvest, cultivate remaining vegetative material into the regulated transgenic site. 

Final Disposition of EUP Material 

Final disposition of EUP seed or grain not saved for further breeding or testing purposes will be 

accomplished using one of the methods below. 

o cultivation into soil at the regulated transgenic site; 

o incineration; 

o burial (at least two feet deep); 

o composting; 

o autoclaving; 

o disposal at an approved landfill. 

Upon full federal and state EPA approval of the EUP Test Plants, all disposition restrictions will 

cease immediately. 

PHI-2008-139 

April 20, 2009Page 222 of 416
Efficacy / IRM Protocol 

General Objectives 

 Assess the efficacy of the EUP Test Plants against key target and secondary pests in corn. 

 Generate data for use in the refinement of IRM strategies for corn containing events 1507, 

MON810 and/or 59122. 

 Characterize the refuge deployment methods for the EUP Test Plants. 

Description 

Test plots will generally be planted in multiple locations using randomized block design, strips or single 

large blocks depending on study type. Seed may be treated with a variety of insecticidal seed 

treatments or blended with non-transgenic or transgenic corn to evaluate various methods of refuge 

deployment. Artificial or natural infestations of lepidopteran and coleopteran may be used in these 

trials. Plants may be treated with herbicide and/or insecticides. Harvested grain is generally not 

retained for breeding purposes. 

Gene Containment 

One or more of the following practices will be used: 

1. Spatial isolation 

Option A. The EUP Block will be separated from any sexually compatible corn by at least 660 

feet. 

Option B. Corn can be grown within 660 feet of an EUP Block. All open-pollinated grain within 

660 feet of the EUP Block will be disposed as stated in “Final Disposition of the EUP Material.” 

Option C. Hand pollinations of non-EUP plants can occur within 660 feet of an EUP Block. Seed 

resulting from such hand-pollinations may be retained for future breeding and product 

development purposes if: 

 1) The hand-pollinated corn plants are pollinated with pollen from non-EUP plants; and 

 2) The ear shoots of the hand-pollinated corn plants are covered for the period of time during 

which the silks of the plants are sexually receptive. 

Remaining open-pollinated grain within 660 feet of the EUP Block will be disposed as stated in 

“Final Disposition of the EUP Material.” 

2. Temporal isolation 

The flowering period for plants within the EUP Block will not coincide with the presence of sexually 

receptive corn within 660 feet. 

3. Reproductive isolation 

The EUP Test Plants and non-registered PIP plants will be detasseled or destroyed prior to the 

onset of anthesis. 

PHI-2008-139 

April 20, 2009Page 223 of 416
Harvest Procedures 

 Hand or machine harvest. 

 Clean combine or picker in the regulated transgenic site. 

 Store retained seed in a clearly labeled and secure facility. 

 After harvest, cultivate remaining vegetative material into the regulated transgenic site. 

Final Disposition of EUP Material 

Final disposition of EUP seed or grain not saved for further breeding or testing purposes will be 

accomplished using one of the methods below. 

o cultivation into soil at the regulated transgenic site; 

o incineration; 

o burial (at least two feet deep); 

o composting; 

o autoclaving; 

o disposal at an approved landfill. 

Upon full federal and state EPA approval of the EUP Test Plants, all disposition restrictions will 

cease immediately. 

PHI-2008-139 

April 20, 2009Page 224 of 416
Efficacy / IRM Protocol with No Isolation 

General Objectives 

 Assess the efficacy of the EUP Test Plants against key target and secondary pests in corn. 

 Generate data for use in the refinement of IRM strategies for corn containing events 1507, 

MON810 and/or 59122. 

 Characterize the refuge deployment methods for the EUP Test Plants. 

Description 

Test plots will generally be planted in multiple locations using randomized block design, strips or single 

large blocks depending on study type. Seed may be treated with a variety of seed treatments and/or 

blended with non-transgenic or transgenic corn. Artificial or natural infestations of lepidopteran and 

coleopteran may be used in these trials. Plants may be treated with herbicide and/or insecticides. 

Harvested grain is generally not retained for breeding purposes. 

Gene Containment 

No containment or isolation procedures are required. Other corn, including commercial corn, may be 

grown within 660 feet of the EUP Block (see Figure 2). Purple-colored corn or an alleyway will 

separate the EUP Block from adjacent corn (see “Planting” language in Section G5). 

Harvest Procedures 

 Hand or machine harvest. 

 Clean combine or picker in the regulated transgenic site. 

 Store retained seed in a clearly labeled and secure facility. 

 After harvest, cultivate remaining vegetative material into the regulated transgenic site. 

Final Disposition of EUP Material 

Final disposition of EUP seed or grain not saved for further breeding or testing purposes will be 

accomplished using one of the methods below. 

o cultivation into soil at the regulated transgenic site; 

o incineration; 

o burial (at least two feet deep); 

o composting; 

o autoclaving; 

o disposal at an approved landfill. 

Upon full federal and state EPA approval of the EUP Test Plants, all disposition restrictions will 

cease immediately. 

PHI-2008-139 

April 20, 2009Page 225 of 416
Seed Production Protocol 

General Objectives 

 Increase inbred and hybrid seed for further breeding and testing. 

Description 

EUP Blocks (approximately 0.1 to 15 acres in size) of individual inbred lines may be increased by 

open pollination. Hybrid seed may be produced (in EUP Blocks approximately 1 to 30 acres in 

size) by planting two inbred lines per hybrid produced and allowing open pollination (by using a 

male sterile inbred or removing the tassels from one of the inbreds). Plants may be treated with 

herbicides and/or insecticides. 

Gene Containment 

One or more of the following practices will be used: 

1. Spatial isolation 

Option A. The EUP Block will be separated from any sexually compatible corn by at least 

660 feet. 

Option B. Corn can be grown within 660 feet of an EUP Block. All open-pollinated grain 

within 660 feet of the EUP Block will be disposed as stated in “Final Disposition of the EUP 

Material.” 

Option C. Hand pollinations of non-EUP plants can occur within 660 feet of an EUP Block. 

Seed resulting from such hand-pollinations may be retained for future breeding and product 

development purposes if: 

 1) The hand-pollinated corn plants are pollinated with pollen from non-EUP plants; and 

 2) The ear shoots of the hand-pollinated corn plants are covered for the period of time 

during which the silks of the plants are sexually receptive. 

Remaining open-pollinated grain within 660 feet of the EUP Block will be disposed as stated 

in “Final Disposition of the EUP Material.” 

2. Temporal isolation 

The flowering period for plants within the EUP Block will not coincide with the presence of 

sexually receptive corn within 660 feet. 

3. Reproductive isolation 

The EUP Test Plants and non-registered PIP plants will be detasseled or destroyed prior to 

the onset of anthesis. 

Harvest Procedures 

 Hand or machine harvest. 

 Clean combine or picker in the regulated transgenic site. 

 Store retained seed in a clearly labeled and secure facility. 

 After harvest, cultivate remaining vegetative material into the regulated transgenic site. 

PHI-2008-139 

April 20, 2009Page 226 of 416
Final Disposition of EUP Material 

Unwanted seed, grain and plant material (such as cobs and husks) may be used for animal feed 

and/or bedding. Such material may also be disposed of using one of the methods below: 

o cultivation into soil at the regulated transgenic site; 

o incineration; 

o burial (at least two feet deep); 

o composting; 

o autoclaving; 

o disposal at an approved landfill. 

Upon full federal and state EPA approval of the EUP Test Plants, all disposition restrictions will 

cease immediately. 

PHI-2008-139 

April 20, 2009Page 227 of 416
Regulatory Protocol 

General Objectives: 

 Conduct field trials as needed to support global regulatory submissions

 Generate plant tissue samples from EUP Test Plants (inbreds and hybrids) for use in 

regulatory characterization studies.

 Collect phenotypic data for use in global regulatory submissions

 Produce grain for use in animal feeding studies

Description 

Test plots will generally be planted in multiple locations using randomized block design, strips or 

single large blocks depending on study type. Agronomic characteristics and plant tissue samples 

may be collected at various developmental growth stages. Plants may be treated with herbicide 

and/or insecticides. 

Gene Containment 

One or more of the following practices will be used: 

1. Spatial isolation 

Option A. The EUP Block will be separated from any sexually compatible corn by at least 

660 feet. 

Option B. Corn can be grown within 660 feet of an EUP Block. All open-pollinated grain 

within 660 feet of the EUP Block will be disposed as stated in “Final Disposition of the EUP 

Material.” 

Option C. Hand pollinations of non-EUP plants can occur within 660 feet of an EUP Block. 

Seed resulting from such hand-pollinations may be retained for future breeding and product 

development purposes if: 

 1) The hand-pollinated corn plants are pollinated with pollen from non-EUP plants; and 

 2) The ear shoots of the hand-pollinated corn plants are covered for the period of time 

during which the silks of the plants are sexually receptive. 

Remaining open-pollinated grain within 660 feet of the EUP Block will be disposed as stated 

in “Final Disposition of the EUP Material.” 

2. Temporal isolation 

The flowering period for plants within the EUP Block will not coincide with the presence of 

sexually receptive corn within 660 feet. 

3. Reproductive isolation 

The EUP Test Plants and non-registered PIP plants will be detasseled or destroyed prior to 

the onset of anthesis. 

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April 20, 2009Page 228 of 416
Harvest Procedures 

 Hand or machine harvest. 

 Clean harvest equipment (i.e., combine) while in the regulated transgenic site. 

 Store retained seed in a clearly labeled and secure facility. 

 After harvest, cultivate remaining vegetative material into the regulated transgenic site. 

Final Disposition of EUP Material 

Final disposition of EUP seed or grain not saved for further breeding or testing purposes will be 

accomplished using one of the methods below. 

o cultivation into soil at the regulated transgenic site; 

o incineration; 

o burial (at least two feet deep); 

o composting; 

o autoclaving; 

o disposal at an approved landfill. 

Upon full federal and state EPA approval of the EUP Test Plants, all disposition restrictions will 

cease immediately. 

PHI-2008-139 

April 20, 2009Page 229 of 416
 

CONFIDENTIAL ATTACHMENT 

[Pages 231-416 are Confidential Business Information and are classified as Category C.] 

PHI-2008-139 

April 20, 2009Page 230 of 416