Source: C/O E AFRICAN VETERINARY RES ORG submitted to 

EVALUATION OF RIFT VALLEY FEVER (RVF) VETERINARY DIAGNOSTIC REAGENTS

Project Director

WILSON W C

Recipient Organization

C/O E AFRICAN VETERINARY RES ORG

(N/A)

KABETE,null null

Performing Department

(N/A)

Non Technical Summary

(N/A)

Animal Health Component 60%

Basic 20%

Applied 60%

Developmental 20%

Research Effort Categories

Classification

Knowledge Area (KA) Subject of Investigation (SOI) Field of Science (FOS) Percent

311 3610 1090 33%

311 3410 1101 33%

311 3310 1090 34%

Knowledge Area

311 - Animal Diseases;

Subject Of Investigation

Sponsoring Institution Agricultural Research

Service/USDA

Project Status TERMINATED

Funding Source USDA COOPERATIVE

AGREEMENT

Reporting Frequency Annual Accession No. 0412498

Grant No. (N/A) Project No. 5430-32000-001-03S

Proposal No. (N/A) Multistate No. (N/A)

Program Code (N/A) Project Start Date Sep 1, 2007

Project End Date Jan 31, 2012 Grant Year (N/A)

EVALUATION OF RIFT VALLEY FEVER (RVF) VETERINARY DI... http://portal.nifa.usda.gov/web/crisprojectpages/0412498-evaluation-of-ri...

1 of 4 2/16/2017 9:37 AM
3610 - Sheep, live animal; 3310 - Beef cattle, live animal; 3410 - Dairy cattle, live animal;

Field Of Science

1090 - Immunology; 1101 - Virology;

Goals / Objectives

The primary objective of this project is to expedite the evaluation and increase the availability of Rift Valley

fever (RVF) veterinary diagnostic reagents and to develop a RVF reference veterinary sample archive.

Project Methods

The aim of this project is to create RVF reference sample archives, enhance ongoing veterinary surveillance,

evaluate existing and new RVF diagnostic test in Kenya. The collaborative project will also evaluate existing and

newly developed diagnostic tests such as an enzyme-linked immunosorbent assay (ELISA) based on a

recombinant antigen developed by mutual FAO/IAEA collaborators. K-DVS will archive veterinary diagnostic and

surveillance samples collected and assayed using existing diagnostic technology as part of their ongoing

projects. The USDA, APHIS, National Wildlife Research Center (NWRC), in collaboration with the US Army

Medical Research Unit-Kenya (USAMRU-K) and the Kenya Medical Research Institute (KEMRI) have collected

and stored serum and tissue samples from wild rodents in Kenya. The ABADRL scientists will work with K-DVS

scientists in Kenyan facilities to gain experience with existing diagnostics, perform the wild rodent sample

analysis, and assist in the evaluation of existing and newly developed diagnostic tests. In addition, the CDC,

International Emerging Infections Program (IEIP)-Kenya has an interest in wildlife RVF serology and has

performed a wildlife sero-survey. The ABADRL, K-DVS and mutually agreed upon collaborating scientists, with

the assistance of the USDA, APHIS, Centers for Epidemiology and Animal Health (CEAH) will consolidate the

information generated in this project with the existing data to initiate the development a comprehensive

epidemiological understanding of RVF in Kenya.

Progress 09/01/07 to 01/31/12

Outputs

Progress Report Objectives (from AD-416): The primary objective of this project is to expedite the evaluation

and increase the availability of Rift Valley fever (RVF) veterinary diagnostic reagents and to develop a RVF

reference veterinary sample archive. Approach (from AD-416): The aim of this project is to create RVF

reference sample archives, enhance ongoing veterinary surveillance, evaluate existing and new RVF

diagnostic test in Kenya. The collaborative project will also evaluate existing and newly developed diagnostic

tests such as an enzyme-linked immunosorbent assay (ELISA) based on a recombinant antigen developed by

mutual FAO/IAEA collaborators. K-DVS will archive veterinary diagnostic and surveillance samples collected

and assayed using existing diagnostic technology as part of their ongoing projects. The USDA, APHIS,

National Wildlife Research Center (NWRC), in collaboration with the US Army Medical Research Unit-Kenya

(USAMRU-K) and the Kenya Medical Research Institute (KEMRI) have collected and stored serum and tissue

samples from wild rodents in Kenya. The ABADRL scientists will work with K-DVS scientists in Kenyan

facilities to gain experience with existing diagnostics, perform the wild rodent sample analysis, and assist in

the evaluation of existing and newly developed diagnostic tests. In addition, the CDC, International Emerging

Infections Program (IEIP)-Kenya has an interest in wildlife RVF serology and has performed a wildlife serosurvey. The ABADRL, K-DVS and mutually agreed upon collaborating scientists, with the assistance of the

USDA, APHIS, Centers for Epidemiology and Animal Health (CEAH) will consolidate the information generated

in this project with the existing data to initiate the development a comprehensive epidemiological

understanding of RVF in Kenya. This project was initiated to provide a collection of veterinary diagnostic

samples characterized by existing RVF diagnostic tools to be utilized in evaluation of new diagnostic tools.

K-DVS Staff were trained in the qRT-PCR and demonstrated competence with the field equipment and are

currently running the assay on new submissions. The K-DVS has also improved the laboratory infrastructure

to better maintain this valuable collection of characterized veterinary samples.

Impacts

(N/A)

Publications

Keywords

rift valley fever rvf

veterinary diagnostic

EVALUATION OF RIFT VALLEY FEVER (RVF) VETERINARY DI... http://portal.nifa.usda.gov/web/crisprojectpages/0412498-evaluation-of-ri...

2 of 4 2/16/2017 9:37 AM
Progress 10/01/10 to 09/30/11

Outputs

Progress Report Objectives (from AD-416) The primary objective of this project is to expedite the evaluation

and increase the availability of Rift Valley fever (RVF) veterinary diagnostic reagents and to develop a RVF

reference veterinary sample archive. Approach (from AD-416) The aim of this project is to create RVF

reference sample archives, enhance ongoing veterinary surveillance, evaluate existing and new RVF

diagnostic test in Kenya. The collaborative project will also evaluate existing and newly developed diagnostic

tests such as an enzyme-linked immunosorbent assay (ELISA) based on a recombinant antigen developed by

mutual FAO/IAEA collaborators. K-DVS will archive veterinary diagnostic and surveillance samples collected

and assayed using existing diagnostic technology as part of their ongoing projects. The USDA, APHIS,

National Wildlife Research Center (NWRC), in collaboration with the US Army Medical Research Unit-Kenya

(USAMRU-K) and the Kenya Medical Research Institute (KEMRI) have collected and stored serum and tissue

samples from wild rodents in Kenya. The ABADRU scientists will work with K-DVS scientists in Kenyan

facilities to gain experience with existing diagnostics, perform the wild rodent sample analysis, and assist in

the evaluation of existing and newly developed diagnostic tests. In addition, the CDC, International Emerging

Infections Program (IEIP)-Kenya has an interest in wildlife RVF serology and has performed a wildlife serosurvey. The ABADRU, K-DVS and mutually agreed upon collaborating scientists, with the assistance of the

USDA, APHIS, Centers for Epidemiology and Animal Health (CEAH) will consolidate the information generated

in this project with the existing data to initiate the development a comprehensive epidemiological

understanding of RVF in Kenya. This project was initiated to provide a collection of veterinary diagnostic

samples characterized by existing RVF diagnostic tools to be utilized in evaluation of new diagnostic tools.

K-DVS Staff were trained in the qRT-PCR and demonstrated competence with the field equipment and are

currently running the assay on new submissions. The K-DVS has also improved the laboratory infrastructure

to better maintain this valuable collection of characterized veterinary samples. This research supports NP103

Action Plan Components 1. Biodefense Research, and 3. Prevent and Control Zoonotic Diseases. ADODR is

directly involved in performance of the research and also monitors activities to evaluate research progress

through site visits, meeting at conferences and through email and phone calls.

Impacts

(N/A)

Publications

Progress 10/01/09 to 09/30/10

Outputs

Progress Report Objectives (from AD-416) The primary objective of this project is to expedite the evaluation

and increase the availability of Rift Valley fever (RVF) veterinary diagnostic reagents and to develop a RVF

reference veterinary sample archive. Approach (from AD-416) The aim of this project is to create RVF

reference sample archives, enhance ongoing veterinary surveillance, evaluate existing and new RVF

diagnostic test in Kenya. The collaborative project will also evaluate existing and newly developed diagnostic

tests such as an enzyme-linked immunosorbent assay (ELISA) based on a recombinant antigen developed by

mutual FAO/IAEA collaborators. K-DVS will archive veterinary diagnostic and surveillance samples collected

and assayed using existing diagnostic technology as part of their ongoing projects. The USDA, APHIS,

National Wildlife Research Center (NWRC), in collaboration with the US Army Medical Research Unit-Kenya

(USAMRU-K) and the Kenya Medical Research Institute (KEMRI) have collected and stored serum and tissue

samples from wild rodents in Kenya. The ABADRL scientists will work with K-DVS scientists in Kenyan

facilities to gain experience with existing diagnostics, perform the wild rodent sample analysis, and assist in

the evaluation of existing and newly developed diagnostic tests. In addition, the CDC, International Emerging

Infections Program (IEIP)-Kenya has an interest in wildlife RVF serology and has performed a wildlife serosurvey. The ABADRL, K-DVS and mutually agreed upon collaborating scientists, with the assistance of the

USDA, APHIS, Centers for Epidemiology and Animal Health (CEAH) will consolidate the information generated

in this project with the existing data to initiate the development a comprehensive epidemiological

understanding of RVF in Kenya. This project was initiated to provide a collection of veterinary diagnostic

samples characterized by existing RVF diagnostic tools to be utilized in evaluation of new diagnostic tools.

K-DVS Staff were trained in the qRT-PCR and demonstrated competence with the field equipment. The K-DVS

equipment however, failed to perform the assay correctly and we were unable to trouble shoot the problem.

EVALUATION OF RIFT VALLEY FEVER (RVF) VETERINARY DI... http://portal.nifa.usda.gov/web/crisprojectpages/0412498-evaluation-of-ri...

3 of 4 2/16/2017 9:37 AM
A collection veterinary samples have been maintained and initial progress toward infrastructure needed to

properly store this collection was done. This research supports NP103 Action Plan Components 1. Biodefense

Research, and 3. Prevent and Control Zoonotic Diseases. ADODR is directly involved in performance of the

research and also monitors activities to evaluate research progress through site visits, meeting at

conferences and through email and phone calls.

Impacts

(N/A)

Publications

Progress 10/01/07 to 09/30/08

Outputs

Progress Report Objectives (from AD-416) The primary objective of this project is to expedite the evaluation

and increase the availability of Rift Valley fever (RVF) veterinary diagnostic reagents and to develop a RVF

reference veterinary sample archive. Approach (from AD-416) The aim of this project is to create RVF

reference sample archives, enhance ongoing veterinary surveillance, evaluate existing and new RVF

diagnostic test in Kenya. The collaborative project will also evaluate existing and newly developed diagnostic

tests such as an enzyme-linked immunosorbent assay (ELISA) based on a recombinant antigen developed by

mutual FAO/IAEA collaborators. K-DVS will archive veterinary diagnostic and surveillance samples collected

and assayed using existing diagnostic technology as part of their ongoing projects. The USDA, APHIS,

National Wildlife Research Center (NWRC), in collaboration with the US Army Medical Research Unit-Kenya

(USAMRU-K) and the Kenya Medical Research Institute (KEMRI) have collected and stored serum and tissue

samples from wild rodents in Kenya. The ABADRL scientists will work with K-DVS scientists in Kenyan

facilities to gain experience with existing diagnostics, perform the wild rodent sample analysis, and assist in

the evaluation of existing and newly developed diagnostic tests. In addition, the CDC, International Emerging

Infections Program (IEIP)-Kenya has an interest in wildlife RVF serology and has performed a wildlife serosurvey. The ABADRL, K-DVS and mutually agreed upon collaborating scientists, with the assistance of the

USDA, APHIS, Centers for Epidemiology and Animal Health (CEAH) will consolidate the information generated

in this project with the existing data to initiate the development a comprehensive epidemiological

understanding of RVF in Kenya. Significant Activities that Support Special Target Populations This project was

initiated to provide a collection of veterinary diagnostic samples characterized by existing Rift Valley Fever

Virus (RVFV) diagnostic tools to be utilized in evaluation of new diagnostic tools. There is a mutual need to

evaluate existing and new RVFV diagnostic tests and increase the availability of RVFV diagnostic reagents.

The ARS Arthropod-Borne Animal Diseases Research Laboratory (ABADRL) is developing new veterinary RVFV

diagnostic reagents to increase their availability for use by veterinary diagnostic laboratories including the

USDA, Animal Plant Health Inspection Service (APHIS), and Foreign Animal Disease Diagnostic Laboratory

(FADDL). The Kenya Department of Veterinary Services, Kabete, Kenya (K-DVS), have ongoing RVFV

diagnostic and evaluation programs, and provide the reference sample collections needed from Kenya (an

endemic country). This research supports NP103 Action Plan Components 1. Biodefense Research, and 3.

Prevent and Control Zoonotic Diseases. ADODR monitoring includes site visits, discussions at scientific

conferences, email correspondence, and phone calls.

Impacts

(N/A)

Publications

EVALUATION OF RIFT VALLEY FEVER (RVF) VETERINARY DI... http://portal.nifa.usda.gov/web/crisprojectpages/0412498-evaluation-of-ri...

4 of 4 2/16/2017 9:37 AM