Title

Application for an Experimental Use Permit for 1507x59122xMON810,

1507xMON810 and 59122xMON810

Data Requirement

40 CFR Part 172

Authors

Rachel Binning, Keri Henderson, Dave Langer, Scott Mundell, Tracy Rood and Jamie Staley

Original Submission Date

August 14, 2008

Submission of Updated Version

April 20, 2009

Submitter

Tracy A. Rood Pioneer Hi-Bred International, Inc.

7250 NW 62 nd Ave. Johnston, IA 50131

Submitter Reference Number

PHI-2008-139

Volume 1Volume 1

Table of Contents

Page

Statement of Data Confidentiality Claim 2

Transmittal Document 5

EPA Form 8570-17 (Application for Experimental Use Permit) 7

EPA Form 8570-4 (Confidential Statements of Formula) 8

EPA Form 8570-34 (Certification with Respect to Citation of Data) 9

EPA Form 8570-35 (Data Matr ix for 1507x5912 2xMON810) 10

EPA Form 8570-35 (Data Ma trix for 1507xMON810) 65

EPA Form 8570-35 (Data Ma trix for 59122xMON810) 98

Section A. Product Chemistry 131

Section B. Proposed Labels 132

Section C. Reference to Available Environmental Toxicity Data 193

Section D. Residue Data 194

Section E. Effectiveness Data 195

Section F. Tolerance if Testing Involves Use in Food or Feed 199

Section G. Proposed Experimental Program 200

G1. Participants in the Experimental Program 200

G2. Acreage and Seed Quantities by Stat e or Territory for the EUP Program 203

G3. Details of the Proposed EUP Program 213

G4. Definitions 213

G5. Universal Protocol Procedures 214

G6. Special Protocol Exemptions 215

Nursery / Breeding Protocol 219

Yield / Agronomic Protocol 221

Efficacy / IRM Protocol 223

Efficacy / IRM Protocol with No Isolation 225

Seed Production Protocol 226

Regulatory Protocol 228

Confidential Attachment 230List of Tables and Figures

Tables

Page

Table 1 Cross-reference Event Table 131

Table 2. Lepidopteran Pests That Are Controlled or Suppressed by Pyramided

1507xMON810 195

Table 3. Efficacy of Breeding Stacks Against First Generation European Corn Borer 197

Table 4. Efficacy of Breeding Stacks Against Fall Armyworm 198

Table 5. List of Participants 200

Table 6. Breeding Stacks Included in Categories 1, 2 and 3 203

Table 7. Proposed Total Acreage by State 205

Table 8. Estimated Acreage per State/Te rritory for Nursery / Breeding Protocol 206

Table 9. Estimated Acreage per State/Te rritory for Yield / Agronomic Protocol 207

Table 10. Estimated Acreage per State/Te rritory for Efficacy / IRM Protocol 208

Table 11. Estimated Acreage per State/Territory for Efficacy / IRM Protocol with No Isolation 209

Table 12. Estimated Acreage per State/Te rritory for Seed Production Protocol 210

Table 13. Estimated Acreage per State/ Territory for Regulatory Protocol 211

Table 14. Summary of EUP Program Quantities for 2009 212

Figures

Figure 1. Example Regulated Transgenic Site Map 216

Figure 2. Example Trial Site Map with No Isolation 217

Figure 3. Example of Master Label Weight Summary 218Transmittal Document

Submitter

Pioneer Hi-Bred International, Inc.

7250 NW 62 nd Ave. Johnston, IA 50131

Regulatory Action in Support of Which This Document is Submitted

Application for an Experimental Use Permit with a Plant-Incorporated Protectant Pursuant to FIFRA §5(a): Application for an Experimental Use Permit for 1507x59122xMON810,

1507xMON810 and 59122XMON810

29964-EUPOriginal Transmittal Date

August 14, 2008

Submission of Updated Version

April 20, 2009

Submitter Reference Number

PHI-2008-139PHI-2008-139 Page 8 of 237 August 14, 2008

EPA Forms 8570-4

Confidential Statement of Formula for 1507x59122xMON810

Confidential Statement of Formula for 1507xMON810

Confidential Statement of Formula for 59122xMON810

{See Confidential Appendix}PHI-2008-139 Page 10 of 230 August 14, 2008

EPA Form 8570-35

Data Matrix for 1507x59122xMON810 (see following pages)PHI-2008-139 Page 65 of 237 August 14, 2008

EPA Form 8570-35

Data Matrix for 1507xMON810 (see following pages)PHI-2008-139 Page 98 of 237 August 14, 2008

EPA Form 8570-35

Data Matrix for 59122xMON810 (see following pages)PHI-2008-139 Page 131 of 237 August 14, 2008

Sections A Through G of the EUP Application

Section A. Product Chemistry

Pioneer is developing two new products that pyrami d Lepidopteran insecticidal corn traits. These products are being made by crossing registered even ts using conventional breeding. The 1507xMON180 product expresses the Cry1F and Cry1Ab Bt-derived protein, each highly selective and effective in controlling certain economically important lepidopteran insect pests of field corn. For markets where control of corn rootworm is necessary, Pionee r is developing the 1507x59122xMON810 product by adding (also by conventional breeding) a third regist ered event that expresses the Cry34/35Ab1 protein, active against corn rootworm larvae. The 59122x MON810 breeding stack is not intended as a commercial product, but rather will be used as a breeding intermediate and/or inbred for product development of the 1507x59122x MON810 breeding stack.

Combining Cry1F and Cry1Ab brings together two distinct modes of action against key lepidopteran pests. Research has shown that Cry1F and Cry1Ab do not share any high-affinity binding sites (Perreia thesis). Pyramiding the 1507 and MO N810 events will reduce the risk of resistance in insects controlled by both events.

Enclosed is an application for an Experimental Use Permit (EUP) for field testing of insect-resistant corn. The application is for new (non-registered) combinations of previously registered events: DAS-Ø15Ø7-1 (also known as 1507), DAS-59122-7 (also known as 59122), and MON-ØØ81Ø-6 (also known as MON810). These combinations are as follows: 1) DAS-Ø15Ø7-1xDAS-59122-7xMO N-ØØ81Ø-6 (1507x59122xMON810) 2) DAS-Ø15Ø7-1xMON-ØØ81Ø-6 (1507xMON810) 3) DAS-59122-7xMON-ØØ81Ø-6 (59122xMON810)

1507 corn expresses the Cry1F protein that is insectic idally active against certain lepidopteran pests of corn. MON810 expresses the Cry1Ab protein that is likewise active against certain lepidopteran pests of corn. In a pyramided breeding combination, these two events express two Cr y proteins for enhanced pest spectrum and insect resistance management/durability. Also included in the EUP is a triple breeding

stack that includes the 59122 even t, which expresses the Cry34/35A b1 protein for control of corn rootworm pests. The 59122xMON810 breeding stack is not intended as a commercial product, but rather will be used as a breeding intermediate and/ or inbred for product development of the 1507x59122xMON810 breeding stack.

Table 1 specifies the registered events that comprise the combinations that are the subject of the EUP:

Table 1. Cross-reference Event Table

OECD Event

Name Event Name

Abbreviation Product Name Registration

Number PIP Expressed

DAS-Ø15Ø7-1 1507 Herculex 1 I 29964-3 Cry1F

DAS-59122-7 59122 Herculex

Rootworm 29964-4 Cry34/35

MON-ØØ81Ø-6 MON810 YieldGard 2 Corn

Borer 524-489 Cry1Ab

1 Herculex ® I Insect Protection technology by Dow AgroScienc es and Pioneer Hi-Bred. Herculex is a registered trademark of Dow AgroSciences LLC.

4 YieldGard® is a registered trademark us ed under license from Monsanto Company.PHI-2008-139 Page 132 of 237 August 14, 2008

Section B. Proposed Labels

The following pages contain the proposed Master Lab el for the EUP, including labels for the EUP Test Plants (1-3), as well as labels for registered PIPs (4-13) and non-registered PIPs (14-34) that will be planted in EUP experiments.

# Master Label

29964-EUP-6

Subparts:

1) 1507 x 59122 x MON810 Corn Seed 2) 1507 x MON810 Corn Seed 3) 59122 x MON810 Corn Seed

4) 1507 Corn Seed 5) 59122 Corn Seed 6) MON810 Corn Seed 7) 1507 x 59122 Corn Seed 8) MIR604 Corn Seed 9) Bt11 Corn Seed 10) Bt11 x MIR604 Corn Seed 11) MIR162 12) Bt11 x MIR162 13) Bt11 x MIR162 x MIR604

14) 1507 x 59122 x MON810 x MIR162 15) 1507 x 59122 x MON810 x MIR604 16) 1507 x 59122 x MIR162 x MIR604 17) 1507 x MON810 x MIR162 x MIR604 18) 59122 x MON810 x MIR162 x MIR604 19) 1507 x 59122 x MIR162 20) 1507 x 59122 x MIR604 21) 1507 x MON810 x MIR162 22) 1507 x MON810 x MIR604 23) 1507 x MIR162 x MIR604 24) 59122 x MON810 x MIR162 25) 59122 x MON810 x MIR604 26) 59122 x MIR162 x MIR604 27) MON810 x MIR162 x MIR604 28) 1507 x MIR162 29) 1507 x MIR604 30) 59122 x MIR162 31) 59122 x MIR604 32) MON810 x MIR162 33) MON810 x MIR604 34) MIR162 x MIR604

## 1) 1507 x 59122 x MON810 Corn Seed

EPA EXPERIMENTAL USE PERM IT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resis tant corn that produces the following proteins with activity against certain lepidopteran and corn r ootworm pests: Cry1F, Cry34/35Ab1, and Cry1Ab.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA- approved Experimental Use Program. For use only at an application site of a Part icipant or Coordinator and in accordance with the terms and conditions of the Experimental Use Permit (E UP). This labeling must be in possession of the user at the time the corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry1F protein in DAS-Ø15Ø7-1 and the genetic material necessary for its producti on in corn................................................................<0 .00036%*

Bacillus thuringiensis Cry34Ab1 protein in DAS-59122-7 and the genetic material necessa ry for its produc tion in corn.................................................................. <0.0036%*

Bacillus thuringiensis Cry35Ab1 protein in DAS-59122-7 and the genetic material necessary for its producti on in corn................................................................<0 .00023%*

Bacillus thuringiensis Cry1Ab protein in MON-ØØ81Ø-6 and the genetic material necessary for its production in corn.............................................................<0.0 00033%*

Inert Ingredient:

A marker protein and the genetic material necessary for its production in event DAS-Ø15Ø7-1 & DAS- 59122-7.................................................<0 .000016%*

* Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Pioneer Hi-Br ed International, Inc.

7300 NW 62 nd Ave. Johnston, IA 50131 NET CONTENTS: ___________pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may only be used according to the field protocols as specified in the approved EUP program. Participants and Cooperators must have a copy of each applicable prot ocol prior to initiating any research with these

seeds.

Research plots must not be located within a 1⁄4 m ile of critical habitats of endangered/threatened lepidopteran and coleopteran species as list ed by the US Fish and Wildlife Service.STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contam inate water, food or feed by storage or disposal. Any seed not used in these experiments must be returned to the seed provider or disposed of as specified in the field protocols. Do not reuse seed containers. En sure that containers are co mpletely empty of seeds before destroying.

## 2) 1507 x MON810 Corn Seed

EPA EXPERIMENTAL USE PERM IT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resis tant corn that produces the following proteins with activity against certain lepidopteran pests: Cry1F and Cry1Ab.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA- approved Experimental Use Program. For use only at an application site of a Part icipant or Coordinator and in accordance with the terms and conditions of the Experimental Use Permit (E UP). This labeling must be in possession of the user at the time the corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry1F protein in DAS-Ø15Ø7-1 and the genetic material necessa ry for its produc tion in corn.................................................................. <0.0003%*

Bacillus thuringiensis Cry1Ab protein in MON-ØØ81Ø-6 and the genetic material necessary for its producti on in co rn..............................................................<0.0 00036%*

Inert Ingredient:

A marker protein and the genetic material necessary for its production in event DAS-Ø1 5Ø7-1......................................................................... <0.00 00069%*

* Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Pioneer Hi-Br ed International, Inc.

7300 NW 62 nd Ave. Johnston, IA 50131

NET CONTENTS: _______________ pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may only be used according to the field protocols as specified in the approved EUP program. Participants and Cooperators must have a copy of each applicable prot ocol prior to initiating any research with these

seeds.

Research plots must not be located within a 1⁄4 m ile of critical habitats of endangered/threatened lepidopteran species as listed by the US Fish and Wildlife Service.STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contam inate water, food or feed by storage or disposal. Any seed not used in these experiments must be returned to the seed provider or disposed of as specified in the field protocols. Do not reuse seed containers. En sure that containers are co mpletely empty of seeds before destroying.

## 3) 59122 x MON810 Corn Seed

EPA EXPERIMENTAL USE PERM IT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resis tant corn that produces the following proteins with activity against certain lepidopteran and corn rootworm pests: Cry34/35Ab1 and Cry1Ab.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA- approved Experimental Use Program. For use only at an application site of a Part icipant or Coordinator and in accordance with the terms and conditions of the Experimental Use Permit (E UP). This labeling must be in possession of the user at the time the corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry34Ab1 protein in DAS-59122-7 and the genetic material necessa ry for its produc tion in corn.................................................................. <0.0045%*

Bacillus thuringiensis Cry35Ab1 protein in DAS-59122-7 and the genetic material necessary for its producti on in corn................................................................<0 .00023%*

Bacillus thuringiensis Cry1Ab protein in MON-ØØ81Ø-6 and the genetic material necessary for its production in corn.............................................................<0.0 00039%*

Inert Ingredient:

A marker protein and the genetic material necessary for its production in event DAS-59 122-7...........................................................................<0. 0000069%*

* Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Pioneer Hi-Br ed International, Inc.

7300 NW 62 nd Ave. Johnston, IA 50131

NET CONTENTS: _______________ pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may only be used according to the field protocols as specified in the approved EUP program. Participants and Cooperators must have a copy of each applicable prot ocol prior to initiating any research with these

seeds.

Research plots must not be located within a 1⁄4 m ile of critical habitats of endangered/threatened lepidopteran and coleopteran species as list ed by the US Fish and Wildlife Service.STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contam inate water, food or feed by storage or disposal. Any seed not used in these experiments must be returned to the seed provider or disposed of as specified in the field protocols. Do not reuse seed containers. En sure that containers are co mpletely empty of seeds before destroying.

## 4) 1507 Corn Seed

EPA EXPERIMENTAL USE PERM IT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resis tant corn that produces the following proteins with activity against certain lepidopteran pests: Cry1F.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA- approved Experimental Use Program. For use only at an application site of a Part icipant or Coordinator and in accordance with the terms and conditions of the Experimental Use Permit (E UP). This labeling must be in possession of the user at the time the corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry1F protein in DAS-Ø15Ø7-1 and the genetic material necessary for its producti on in corn................................................................<0 .01415%*

Inert Ingredient:

A marker protein and the genetic material necessary for its production in event D AS-Ø15Ø7-1.............................................................................. .<0.0020%*

* Maximum percent (wt/wt) of total extractable protein in grain

CAUTION

Keep Out of Reach of Children

EPA REG NO.: 29964-3

EPA Establishment Number: 29964-IA-001 Pioneer Hi-Br ed International, Inc.

7300 NW 62 nd Ave. Johnston, IA 50131

NET CONTENTS: _______________ pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may only be used according to the field protocols as specified in the approved EUP program. Participants and Cooperators must have a copy of each applicable prot ocol prior to initiating any research with these

seeds.

Research plots must not be located within a 1⁄4 m ile of critical habitats of endangered/threatened lepidopteran species as listed by the US Fish and Wildlife Service.

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contam inate water, food or feed by storage or disposal. Any seed not used in these experiments must be returned to the seed provider or disposed of as specified in the field protocols. Do not reuse seed containers. En sure that containers are co mpletely empty of seeds before destroying.

## 5) 59122 Corn Seed

EPA EXPERIMENTAL USE PERM IT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resis tant corn that produces the following proteins with activity against certain corn rootworm pests: Cry34/35Ab1.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA- approved Experimental Use Program. For use only at an application site of a Part icipant or Coordinator and in accordance with the terms and conditions of the Experimental Use Permit (E UP). This labeling must be in possession of the user at the time the corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry34Ab1 protein in DAS-59122-7 and the genetic material necessa ry for its produc tion in corn.................................................................. <0.0117%*

Bacillus thuringiensis Cry35Ab1 protein in DAS-59122-7 and the genetic material necessary for its producti on in co rn..............................................................<0.0 00161%*

Inert Ingredient:

A marker protein and the genetic material necessary for its production in event D AS-59122-7.............................................................................. ...........0.0%*

* Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA REG NO.: 29964-4

EPA Establishment Number: 29964-IA-001 Pioneer Hi-Br ed International, Inc.

7300 NW 62 nd Ave. Johnston, IA 50131 NET CONTENTS: _______________ pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may only be used according to the field protocols as specified in the approved EUP program. Participants and Cooperators must have a copy of each applicable prot ocol prior to initiating any research with these

seeds.

Research plots must not be located within a 1⁄4 m ile of critical habitats of endangered/threatened coleopteran species as listed by t he US Fish and Wildlife Service.

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contam inate water, food or feed by storage or disposal. Any seed not used in these experiments must be returned to the seed provider or disposed of as specified in the field protocols. Do not reuse seed containers. En sure that containers are co mpletely empty of seeds before destroying.

## 6) MON810 Corn Seed

EPA EXPERIMENTAL USE PERM IT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resis tant corn that produces the following proteins with activity against certain lepidopteran pests: Cry1Ab.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA- approved Experimental Use Program. For use only at an application site of a Part icipant or Coordinator and in accordance with the terms and conditions of the Experimental Use Permit (E UP). This labeling must be in possession of the user at the time the corn seed is planted.

Active Ingredient:

Bacillus thuringiensis Cry1Ab protein in MON-ØØ81Ø-6 and the genetic material necessary for its producti on in corn................................................................. <0.0070%*

* Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA REG NO.: 524-489

EPA Establishment Number: 524- MO-002 Monsanto Company. 700 Chesterfield Parkway North. St. Louis, Missouri 63198

NET CONTENTS: _______________ pounds of corn seed

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may only be used according to the field protocols as specified in the approved EUP program. Participants and Cooperators must have a copy of each applicable prot ocol prior to initiating any research with these

seeds.

Research plots must not be located within a 1⁄4 m ile of critical habitats of endangered/threatened lepidopteran species as listed by the US Fish and Wildlife Service.

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contam inate water, food or feed by storage or disposal. Any seed not used in these experiments must be returned to the seed provider or disposed of as specified in the field protocols. Do not reuse seed containers. En sure that containers are co mpletely empty of seeds before destroying.

## 7) 1507 x 59122 Corn Seed

EPA EXPERIMENTAL USE PERM IT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resis tant corn that produces the following proteins with activity against certain lepidopteran and corn rootworm pests: Cry1F and Cry34/35Ab1.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA- approved Experimental Use Program. For use only at an application site of a Part icipant or Coordinator and in accordance with the terms and conditions of the Experimental Use Permit (E UP). This labeling must be in possession of the user at the time the corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry1F protein in DAS-Ø15Ø7-1 and the genetic material necessary for its producti on in corn................................................................<0 .00038%*

Bacillus thuringiensis Cry34Ab1 protein in DAS-59122-7 and the genetic material necessary for its producti on in corn................................................................<0 .00633%*

Bacillus thuringiensis Cry35Ab1 protein in DAS-59122-7 and the genetic material necessary for its producti on in corn................................................................<0 .00034%*

Inert Ingredient:

A marker protein and the genetic material necessary for its production in DAS-Ø 15Ø7-1 & DAS-59122-7 corn.....................................................<0 .00004%*

* Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA REG NO.: 29964-5

EPA Establishment Number: 29964-IA-001 Pioneer Hi-Br ed International, Inc.

7300 NW 62 nd Ave. Johnston, IA 50131

NET CONTENTS: _______________ pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may only be used according to the field protocols as specified in the approved EUP program. Participants and Cooperators must have a copy of each applicable prot ocol prior to initiating any research with these

seeds.

Research plots must not be located within a 1⁄4 m ile of critical habitats of endangered/threatened lepidopteran and coleopteran species as list ed by the US Fish and Wildlife Service.STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contam inate water, food or feed by storage or disposal. Any seed not used in these experiments must be returned to the seed provider or disposed of as specified in the field protocols. Do not reuse seed containers. En sure that containers are co mpletely empty of seeds before destroying.

## 8) MIR604 Corn Seed

EPA EXPERIMENTAL USE PERM IT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resis tant corn that produces the following proteins with activity against corn rootworm pests: mCry3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA- approved Experimental Use Program. For use only at an application site of a Part icipant or Coordinator and in accordance with the terms and conditions of the Experimental Use Permit (E UP). This labeling must be in possession of the user at the time the corn seed is planted.

Active Ingredient:

Bacillus thuringiensis mCry3A protein in SYN-IR6Ø4-5 and the genetic material necessary fo r its production in corn.......................................................<0 .0000003 13%*

Inert Ingredient:

A marker protein and the genetic material necessary for its producti on in event SYN-IR6 Ø4-5 .............................................................................. <0.00060%*

* Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA REG NO.: 67979-5

EPA Establishment Number: 66736-N C-001 Syngenta Seeds Inc.

P.O. Box 12257

3054 East Cornwallis Rd. Research Triangle Park, NC 27709

NET CONTENTS: _______________ pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may only be used according to the field protocols as specified in the approved EUP program. Participants and Cooperators must have a copy of each applicable prot ocol prior to initiating any research with these

seeds.

Research plots must not be located within a 1⁄4 m ile of critical habitats of endangered/threatened coleopteran species as listed by t he US Fish and Wildlife Service.

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contam inate water, food or feed by storage or disposal. Any seed not used in these experiments must be returned to the seed provider or disposed of as specified in the field protocols. Do not reuse seed containers. En sure that containers are co mpletely empty of seeds before destroying.

## 9) Bt11 Corn Seed

EPA EXPERIMENTAL USE PERM IT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resis tant corn that produces the following proteins with activity against certain lepidopteran pests: Cry1Ab.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA- approved Experimental Use Program. For use only at an application site of a Part icipant or Coordinator and in accordance with the terms and conditions of the Experimental Use Permit (E UP). This labeling must be in possession of the user at the time the corn seed is planted.

Active Ingredient:

Bacillus thuringiensis Cry1Ab protein in SYN-BTØ11-1 and the genetic material necessary for its producti on in corn ................................................................. <0.0016%*

Inert Ingredient:

A marker protein and the genetic material necessary for its production in event SYN-B TØ11-1 ...........................................................................<0. 000004%*

* Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA REG NO.: 67979-1

EPA Establishment Number: 66736-N C-001 Syngenta Seeds Inc.

P.O. Box 12257

3054 East Cornwallis Rd. Research Triangle Park, NC 27709

NET CONTENTS: _______________ pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may only be used according to the field protocols as specified in the approved EUP program. Participants and Cooperators must have a copy of each applicable prot ocol prior to initiating any research with these

seeds.

Research plots must not be located within a 1⁄4 m ile of critical habitats of endangered/threatened lepidopteran species as listed by the US Fish and Wildlife Service.

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contam inate water, food or feed by storage or disposal. Any seed not used in these experiments must be returned to the seed provider or disposed of as specified in the field protocols. Do not reuse seed containers. En sure that containers are co mpletely empty of seeds before destroying.

## 10) Bt11 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERM IT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resis tant corn that produces the following proteins with activity against certain lepidopteran and corn rootworm pests: Cry1Ab and mCry3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA- approved Experimental Use Program. For use only at an application site of a Part icipant or Coordinator and in accordance with the terms and conditions of the Experimental Use Permit (E UP). This labeling must be in possession of the user at the time the corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry1Ab protein in SYN-BTØ11-1 and the genetic material necessary for its producti on in corn................................................................<0 .00019%*

Bacillus thuringiensis mCry3A protein in SYN-IR6Ø4-5 and the genetic material necessary fo r its production in corn........................................................<0 .0000000 08%*

Inert Ingredients:

A marker protein and the genetic material necessary for its production in event SYN-B TØ11-1 ...........................................................................<0. 000004%*

A marker protein and the genetic material necessary for its producti on in event SYN-IR6 Ø4-5 .............................................................................. <0.00004%*

* Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA REG NO: 67979-8

EPA Establishment Number: 66736-N C-001 Syngenta Seeds Inc.

P.O. Box 12257

3054 East Cornwallis Rd. Research Triangle Park, NC 27709

NET CONTENTS: _______________ pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may only be used according to the field protocols as specified in the approved EUP program. Participants and Cooperators must have a copy of each applicable prot ocol prior to initiating any research with these

seeds.

Research plots must not be located within a 1⁄4 m ile of critical habitats of endangered/threatened lepidopteran and coleopteran species as list ed by the US Fish and Wildlife Service.STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contam inate water, food or feed by storage or disposal. Any seed not used in these experiments must be returned to the seed provider or disposed of as specified in the field protocols. Do not reuse seed containers. En sure that containers are co mpletely empty of seeds before destroying.

## 11) MIR162 Corn Seed

EPA EXPERIMENTAL USE PERM IT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resis tant corn that produces the following proteins with activity against certain lepidopteran pests: VIP3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA- approved Experimental Use Program. For use only at an application site of a Part icipant or Coordinator and in accordance with the terms and conditions of the Experimental Use Permit (E UP). This labeling must be in possession of the user at the time the corn seed is planted.

Active Ingredient:

Bacillus thuringiensis Vip3Aa20 protein in SYN-IR162-4 and the genetic material necessary for its producti on in corn................................................................<0 .00403%*

Inert Ingredient:

A marker protein and the genetic material necessary for its producti on in event SYN-IR1 62-4.............................................................................. .<0.00019%*

* Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 66736-N C-001 Syngenta Seeds Inc.

P.O. Box 12257

3054 East Cornwallis Rd. . Research Triangle Park, NC 27709

NET CONTENTS: ___________ pounds of corn seed

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may only be used according to the field protocols as specified in the approved EUP program. Participants and Cooperators must have a copy of each applicable prot ocol prior to initiating any research with these

seeds.

Research plots must not be located within a 1⁄4 m ile of critical habitats of endangered/threatened lepidopteran species as listed by the US Fish and Wildlife Service.

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contam inate water, food or feed by storage or disposal. Any seed not used in these experiments must be returned to the seed provider or disposed of as specified in the field protocols. Do not reuse seed containers. En sure that containers are co mpletely empty of seeds before destroying.

## 12) Bt11 x MIR162 Corn Seed

EPA EXPERIMENTAL USE PERM IT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resis tant corn that produces the following proteins with activity against certain lepidopteran pests: Cry1Ab and VIP3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA- approved Experimental Use Program. For use only at an application site of a Part icipant or Coordinator and in accordance with the terms and conditions of the Experimental Use Permit (E UP). This labeling must be in possession of the user at the time the corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry1Ab protein in SYN-BTØ11-1 and the genetic material necessa ry for its produc tion in corn.................................................................. <0.0011%*

Bacillus thuringiensis Vip3Aa20 protein in SYN-IR162-4 and the genetic material necessa ry for its produc tion in corn.................................................................. <0.0102%*

Inert Ingredients:

A marker protein and the genetic material necessary for its production in event SYN-B TØ11-1 ...........................................................................<0. 000041%*

A marker protein and the genetic material necessary for its production in event SYN -IR162-4.............................................................................< 0.000041%*

* Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 66736-N C-001 Syngenta Seeds Inc.

P.O. Box 12257

3054 East Cornwallis Rd. Research Triangle Park, NC 27709

NET CONTENTS: _______________ pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may only be used according to the field protocols as specified in the approved EUP program. Participants and Cooperators must have a copy of each applicable prot ocol prior to initiating any research with these

seeds.

Research plots must not be located within a 1⁄4 m ile of critical habitats of endangered/threatened lepidopteran species as listed by the US Fish and Wildlife Service.STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contam inate water, food or feed by storage or disposal. Any seed not used in these experiments must be returned to the seed provider or disposed of as specified in the field protocols. Do not reuse seed containers. En sure that containers are co mpletely empty of seeds before destroying.

## 13) Bt11 x MIR162 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERM IT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resis tant corn that produces the following proteins with activity against certain lepidopteran and corn rootworm pests: Cry1Ab, VIP3A and mCry3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA- approved Experimental Use Program. For use only at an application site of a Part icipant or Coordinator and in accordance with the terms and conditions of the Experimental Use Permit (E UP). This labeling must be in possession of the user at the time the corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry1Ab protein in SYN-BTØ11-1 and the genetic material necessary for its producti on in corn................................................................<0 .00025%*

Bacillus thuringiensis Vip3Aa20 protein in SYN-IR162-4 and the genetic material necessa ry for its produc tion in corn.................................................................. <0.0165%*

Bacillus thuringiensis mCry3A protein in SYN-IR6Ø4-5 and the genetic material necessary fo r its production in corn......................................................<0 .000000007 7%*

Inert Ingredients:

A marker protein and the genetic material necessary for its production in event SYN -BTØ11-1 .............................................................................< 0.00003%*

A marker protein and the genetic material necessary for its production in event SYN-IR162-4 & SYN-IR6 Ø4-5.....................................................<0 .00065%*

* Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 66736-N C-001 Syngenta Seeds Inc.

P.O. Box 12257

3054 East Cornwallis Rd. Research Triangle Park, NC 27709

NET CONTENTS: _______________ pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may only be used according to the field protocols as specified in the approved EUP program. Participants and Cooperators must have a copy of each applicable prot ocol prior to initiating any research with these

seeds.

Research plots must not be located within a 1⁄4 m ile of critical habitats of endangered/threatened lepidopteran and coleopteran species as list ed by the US Fish and Wildlife Service.STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contam inate water, food or feed by storage or disposal. Any seed not used in these experiments must be returned to the seed provider or disposed of as specified in the field protocols. Do not reuse seed containers. En sure that containers are co mpletely empty of seeds before destroying.

## 14) 1507 x 59122 x MON810 x MIR162 Corn Seed

EPA EXPERIMENTAL USE PERM IT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resis tant corn that produces the following proteins with activity against certain lepidopteran and corn rootwo rm pests: Cry1F, Cry34/35Ab1, Cry1Ab and VIP3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA- approved Experimental Use Program. For use only at an application site of a Part icipant or Coordinator and in accordance with the terms and conditions of the Experimental Use Permit (E UP). This labeling must be in possession of the user at the time the corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry1F protein in DAS-Ø15Ø7-1 and the genetic material necessary for its producti on in corn................................................................<0 .01415%*

Bacillus thuringiensis Cry34Ab1 protein in DAS-59122-7 and the genetic material necessary for its producti on in corn................................................................ <0 .0117%**

Bacillus thuringiensis Cry35Ab1 protein in DAS-59122-7 and the genetic material necessary for its production in corn ............................................................ <0.000 161%**

Bacillus thuringiensis Cry1Ab protein in MON-ØØ81Ø-6 and the genetic material necessary for its producti on in corn............................................................... <0 .0070%**

Bacillus thuringiensis Vip3Aa20 protein in SYN-IR162-4 and the genetic material necessary for its producti on in co rn.............................................................. <0.0 0403%**

Inert Ingredients:

A marker protein and the genetic material necessary for its production in event DAS-Ø15Ø7-1 & DAS-59122- 7.............................................................0.0 %*

A marker protein and the genetic material necessary for its production in event SYN -IR162-4............................................................................. < 0.00019%**

* Maximum percent (wt/wt) of total extractable protein in grain
** Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Pioneer Hi-Br ed International, Inc.

7300 NW 62 nd Ave. Johnston, IA 50131 NET CONTENTS: ___________pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may only be used according to the field protocols as specified in the approved EUP program. Participants and Cooperators must have a copy of each applicable prot ocol prior to initiating any research with these

seeds.Research plots must not be located within a 1⁄4 m ile of critical habitats of endangered/threatened lepidopteran and coleopteran species as list ed by the US Fish and Wildlife Service.

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contam inate water, food or feed by storage or disposal. Any seed not used in these experiments must be returned to the seed provider or disposed of as specified in the field protocols. Do not reuse seed containers. En sure that containers are co mpletely empty of seeds before destroying.

## 15) 1507 x 59122 x MON810 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERM IT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resis tant corn that produces the following proteins with activity against certain lepidopteran and corn rootwo rm pests: Cry1F, Cry34/35Ab1, Cry1Ab and mCry3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA- approved Experimental Use Program. For use only at an application site of a Part icipant or Coordinator and in accordance with the terms and conditions of the Experimental Use Permit (E UP). This labeling must be in possession of the user at the time the corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry1F protein in DAS-Ø15Ø7-1 and the genetic material necessary for its producti on in corn................................................................<0 .01415%*

Bacillus thuringiensis Cry34Ab1 protein in DAS-59122-7 and the genetic material necessary for its producti on in corn................................................................ <0 .0117%**

Bacillus thuringiensis Cry35Ab1 protein in DAS-59122-7 and the genetic material necessary for its production in corn ............................................................ <0.000 161%**

Bacillus thuringiensis Cry1Ab protein in MON-ØØ81Ø-6 and the genetic material necessary for its producti on in corn............................................................... <0 .0070%**

Bacillus thuringiensis mCry3A protein in SYN-IR6Ø4-5 and the genetic material necessary fo r its production in corn...................................................... <0 .000000313 %**

Inert Ingredients:

A marker protein and the genetic material necessary for its production in event DAS-Ø15Ø7-1 & DAS-59122- 7.............................................................0.0 %*

A marker protein and the genetic material necessary for its production in event SYN -IR6Ø4-5 ............................................................................ <0 .00019%**

* Maximum percent (wt/wt) of total extractable protein in grain
** Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Pioneer Hi-Br ed International, Inc.

7300 NW 62 nd Ave. Johnston, IA 50131 NET CONTENTS: ___________pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may only be used according to the field protocols as specified in the approved EUP program. Participants and Cooperators must have a copy of each applicable prot ocol prior to initiating any research with these

seeds.Research plots must not be located within a 1⁄4 m ile of critical habitats of endangered/threatened lepidopteran and coleopteran species as list ed by the US Fish and Wildlife Service.

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contam inate water, food or feed by storage or disposal. Any seed not used in these experiments must be returned to the seed provider or disposed of as specified in the field protocols. Do not reuse seed containers. En sure that containers are co mpletely empty of seeds before destroying.

## 16) 1507 x 59122 x MIR162 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERM IT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resis tant corn that produces the following proteins with activity against certain lepidopteran and corn rootwo rm pests: Cry1F, Cry34/35Ab1, VIP3A and mCry3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA- approved Experimental Use Program. For use only at an application site of a Part icipant or Coordinator and in accordance with the terms and conditions of the Experimental Use Permit (E UP). This labeling must be in possession of the user at the time the corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry1F protein in DAS-Ø15Ø7-1 and the genetic material necessary for its producti on in corn................................................................<0 .01415%*

Bacillus thuringiensis Cry34Ab1 protein in DAS-59122-7 and the genetic material necessary for its producti on in corn................................................................ <0 .0117%**

Bacillus thuringiensis Cry35Ab1 protein in DAS-59122-7 and the genetic material necessary for its production in corn ............................................................ <0.000 161%**

Bacillus thuringiensis Vip3Aa20 protein in SYN-IR162-4 and the genetic material necessary for its production in corn ............................................................. <0.0 0403%**

Bacillus thuringiensis mCry3A protein in SYN-IR6Ø4-5 and the genetic material necessary fo r its production in corn...................................................... <0 .000000313 %**

Inert Ingredients:

A marker protein and the genetic material necessary for its production in D AS-Ø15Ø7-1 & DAS- 59122-7 co rn...............................................................0. 0%*

A marker protein and the genetic material necessary for its production in event SYN-I R6Ø4-5 & SYN-IR162-4 corn.......................................... <0.00019%**

* Maximum percent (wt/wt) of total extractable protein in grain
** Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Pioneer Hi-Br ed International, Inc.

7300 NW 62 nd Ave. Johnston, IA 50131 NET CONTENTS: ___________pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may only be used according to the field protocols as specified in the approved EUP program. Participants and Cooperators must have a copy of each applicable prot ocol prior to initiating any research with these

seeds.Research plots must not be located within a 1⁄4 m ile of critical habitats of endangered/threatened lepidopteran and coleopteran species as list ed by the US Fish and Wildlife Service.

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contam inate water, food or feed by storage or disposal. Any seed not used in these experiments must be returned to the seed provider or disposed of as specified in the field protocols. Do not reuse seed containers. En sure that containers are co mpletely empty of seeds before destroying.

## 17) 1507 x MON810 x MIR162 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERM IT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resis tant corn that produces the following proteins with activity against certain lepidopteran and corn root worm pests: Cry1F, Cry1Ab, VIP3A and mCry3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA- approved Experimental Use Program. For use only at an application site of a Part icipant or Coordinator and in accordance with the terms and conditions of the Experimental Use Permit (E UP). This labeling must be in possession of the user at the time the corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry1F protein in DAS-Ø15Ø7-1 and the genetic material necessary for its producti on in corn................................................................<0 .01415%*

Bacillus thuringiensis Cry1Ab protein in MON-ØØ81Ø-6 and the genetic material necessary for its producti on in corn............................................................... <0 .0070%**

Bacillus thuringiensis Vip3Aa20 protein in SYN-IR162-4 and the genetic material necessary for its producti on in co rn.............................................................. <0.0 0403%**

Bacillus thuringiensis mCry3A protein in SYN-IR6Ø4-5 and the genetic material necessary fo r its production in corn...................................................... <0 .000000313 %**

Inert Ingredients:

A marker protein and the genetic material necessary for its production in event D AS-Ø15Ø7-1.............................................................................. .........0.0%*

A marker protein and the genetic material necessary for its production in event SYN -IR6Ø4-5 & SYN-IR1 62-4 .................................................. <0 .00019%**

* Maximum percent (wt/wt) of total extractable protein in grain
** Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Pioneer Hi-Br ed International, Inc.

7300 NW 62 nd Ave. Johnston, IA 50131 NET CONTENTS: ___________pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may only be used according to the field protocols as specified in the approved EUP program. Participants and Cooperators must have a copy of each applicable prot ocol prior to initiating any research with these

seeds.

Research plots must not be located within a 1⁄4 m ile of critical habitats of endangered/threatened lepidopteran and coleopteran species as list ed by the US Fish and Wildlife Service.STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contam inate water, food or feed by storage or disposal. Any seed not used in these experiments must be returned to the seed provider or disposed of as specified in the field protocols. Do not reuse seed containers. En sure that containers are co mpletely empty of seeds before destroying.

## 18) 59122 x MON810 x MIR162 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERM IT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resis tant corn that produces the following proteins with activity against certain lepidopteran and corn rootwo rm pests: Cry34/35Ab1, Cry1Ab, VIP3A and mCry3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA- approved Experimental Use Program. For use only at an application site of a Part icipant or Coordinator and in accordance with the terms and conditions of the Experimental Use Permit (E UP). This labeling must be in possession of the user at the time the corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry34Ab1 protein in DAS-59122-7 and the genetic material necessa ry for its produc tion in corn.................................................................. <0.0117%*

Bacillus thuringiensis Cry35Ab1 protein in DAS-59122-7 and the genetic material necessary for its producti on in co rn..............................................................<0.0 00161%*

Bacillus thuringiensis Cry1Ab protein in MON-ØØ81Ø-6 and the genetic material necessary for its producti on in corn................................................................. <0.0070%*

Bacillus thuringiensis Vip3Aa20 protein in SYN-IR162-4 and the genetic material necessary for its producti on in corn................................................................<0 .00403%*

Bacillus thuringiensis mCry3A protein in SYN-IR6Ø4-5 and the genetic material necessary fo r its production in corn........................................................<0 .0000003 13%*

Inert Ingredients:

A marker protein and the genetic material necessary for its production in event D AS-59122-7.............................................................................. ...........0.0%*

A marker protein and the genetic material necessary for its production in event SYN -IR6Ø4-5 & SYN-I R162-4....................................................<0 .00060%*

* Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Pioneer Hi-Br ed International, Inc.

7300 NW 62 nd Ave. Johnston, IA 50131 NET CONTENTS: ___________pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may only be used according to the field protocols as specified in the approved EUP program. Participants and Cooperators must have a copy of each applicable prot ocol prior to initiating any research with these

seeds.Research plots must not be located within a 1⁄4 m ile of critical habitats of endangered/threatened lepidopteran and coleopteran species as list ed by the US Fish and Wildlife Service.

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contam inate water, food or feed by storage or disposal. Any seed not used in these experiments must be returned to the seed provider or disposed of as specified in the field protocols. Do not reuse seed containers. En sure that containers are co mpletely empty of seeds before destroying.

## 19) 1507 x 59122 x MI R162 Corn Seed

EPA EXPERIMENTAL USE PERM IT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resis tant corn that produces the following proteins with activity against certain lepidopteran and corn r ootworm pests: Cry1F, Cry34/35Ab1, and VIP3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA- approved Experimental Use Program. For use only at an application site of a Part icipant or Coordinator and in accordance with the terms and conditions of the Experimental Use Permit (E UP). This labeling must be in possession of the user at the time the corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry1F protein in DAS-Ø15Ø7-1 and the genetic material necessary for its producti on in corn................................................................<0 .01415%*

Bacillus thuringiensis Cry34Ab1 protein in DAS-59122-7 and the genetic material necessary for its producti on in corn................................................................ <0 .0117%**

Bacillus thuringiensis Cry35Ab1 protein in DAS-59122-7 and the genetic material necessary for its production in corn ............................................................ <0.000 161%**

Bacillus thuringiensis Vip3Aa20 protein in SYN-IR162-4 and the genetic material necessary for its producti on in co rn.............................................................. <0.0 0403%**

Inert Ingredients:

A marker protein and the genetic material necessary for its production in D AS-Ø15Ø7-1 & DAS- 59122-7 co rn...............................................................0. 0%*

A marker protein and the genetic material necessary for its production in event SYN -IR162-4............................................................................. < 0.00019%**

* Maximum percent (wt/wt) of total extractable protein in grain
** Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Pioneer Hi-Br ed International, Inc.

7300 NW 62 nd Ave. Johnston, IA 50131 NET CONTENTS: ___________pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may only be used according to the field protocols as specified in the approved EUP program. Participants and Cooperators must have a copy of each applicable prot ocol prior to initiating any research with these

seeds.

Research plots must not be located within a 1⁄4 m ile of critical habitats of endangered/threatened lepidopteran and coleopteran species as list ed by the US Fish and Wildlife Service.STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contam inate water, food or feed by storage or disposal. Any seed not used in these experiments must be returned to the seed provider or disposed of as specified in the field protocols. Do not reuse seed containers. En sure that containers are co mpletely empty of seeds before destroying

## 20) 1507 x 59122 x MI R604 Corn Seed

EPA EXPERIMENTAL USE PERM IT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resis tant corn that produces the following proteins with activity against certain lepidopteran and corn r ootworm pests: Cry1F, Cry34/35Ab1 and mCry3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA- approved Experimental Use Program. For use only at an application site of a Part icipant or Coordinator and in accordance with the terms and conditions of the Experimental Use Permit (E UP). This labeling must be in possession of the user at the time the corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry1F protein in DAS-Ø15Ø7-1 and the genetic material necessary for its producti on in corn................................................................<0 .01415%*

Bacillus thuringiensis Cry34Ab1 protein in DAS-59122-7 and the genetic material necessary for its producti on in corn................................................................ <0 .0117%**

Bacillus thuringiensis Cry35Ab1 protein in DAS-59122-7 and the genetic material necessary for its production in corn ............................................................ <0.000 161%**

Bacillus thuringiensis mCry3A protein in SYN-IR6Ø4-5 and the genetic material necessary fo r its production in corn...................................................... <0 .000000313 %**

Inert Ingredients:

A marker protein and the genetic material necessary for its production in D AS-Ø15Ø7-1 & DAS- 59122-7 co rn...............................................................0. 0%*

A marker protein and the genetic material necessary for its production in event SYN -IR6Ø4-5 ............................................................................ <0 .00060%**

* Maximum percent (wt/wt) of total extractable protein in grain
** Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Pioneer Hi-Br ed International, Inc.

7300 NW 62 nd Ave. Johnston, IA 50131 NET CONTENTS: ___________pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may only be used according to the field protocols as specified in the approved EUP program. Participants and Cooperators must have a copy of each applicable prot ocol prior to initiating any research with these

seeds.

Research plots must not be located within a 1⁄4 m ile of critical habitats of endangered/threatened lepidopteran and coleopteran species as list ed by the US Fish and Wildlife Service.STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contam inate water, food or feed by storage or disposal. Any seed not used in these experiments must be returned to the seed provider or disposed of as specified in the field protocols. Do not reuse seed containers. En sure that containers are co mpletely empty of seeds before destroying.

## 21) 1507 x MON810 x MIR162 Corn Seed

EPA EXPERIMENTAL USE PERM IT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resis tant corn that produces the following proteins with activity against certain lepidopteran pe sts: Cry1F, Cry1Ab and VIP3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA- approved Experimental Use Program. For use only at an application site of a Part icipant or Coordinator and in accordance with the terms and conditions of the Experimental Use Permit (E UP). This labeling must be in possession of the user at the time the corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry1F protein in DAS-Ø15Ø7-1 and the genetic material necessary for its producti on in corn................................................................<0 .01415%*

Bacillus thuringiensis Cry1Ab protein in MON-ØØ81Ø-6 and the genetic material necessary for its producti on in corn............................................................... <0 .0070%**

Bacillus thuringiensis Vip3Aa20 protein in SYN-IR162-4 and the genetic material necessary for its producti on in co rn.............................................................. <0.0 0403%**

Inert Ingredients:

A marker protein and the genetic material necessary for its production in event D AS-Ø15Ø7-1.............................................................................. .<0.0020%*

A marker protein and the genetic material necessary for its production in event SYN -IR162-4............................................................................. < 0.00019%**

* Maximum percent (wt/wt) of total extractable protein in grain
** Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Pioneer Hi-Br ed International, Inc.

7300 NW 62 nd Ave. Johnston, IA 50131 NET CONTENTS: ___________pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may only be used according to the field protocols as specified in the approved EUP program. Participants and Cooperators must have a copy of each applicable prot ocol prior to initiating any research with these

seeds.

Research plots must not be located within a 1⁄4 m ile of critical habitats of endangered/threatened lepidopteran species as listed by the US Fish and Wildlife Service.STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contam inate water, food or feed by storage or disposal. Any seed not used in these experiments must be returned to the seed provider or disposed of as specified in the field protocols. Do not reuse seed containers. En sure that containers are co mpletely empty of seeds before destroying

## 22) 1507 x MON810 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERM IT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resis tant corn that produces the following proteins with activity against certain lepidopteran and corn r ootworm pests: Cry1F, Cry1Ab and mCry3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA- approved Experimental Use Program. For use only at an application site of a Part icipant or Coordinator and in accordance with the terms and conditions of the Experimental Use Permit (E UP). This labeling must be in possession of the user at the time the corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry1F protein in DAS-Ø15Ø7-1 and the genetic material necessary for its producti on in corn................................................................<0 .01415%*

Bacillus thuringiensis Cry1Ab protein in MON-ØØ81Ø-6 and the genetic material necessary for its producti on in corn............................................................... <0 .0070%**

Bacillus thuringiensis mCry3A protein in SYN-IR6Ø4-5 and the genetic material necessary fo r its production in corn...................................................... <0 .000000313 %**

Inert Ingredients:

A marker protein and the genetic material necessary for its production in event D AS-Ø15Ø7-1.............................................................................. .<0.0020%*

A marker protein and the genetic material necessary for its production in event SYN -IR6Ø4-5 ............................................................................ <0 .00060%**

* Maximum percent (wt/wt) of total extractable protein in grain
** Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Pioneer Hi-Br ed International, Inc.

7300 NW 62 nd Ave. Johnston, IA 50131 NET CONTENTS: ___________pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may only be used according to the field protocols as specified in the approved EUP program. Participants and Cooperators must have a copy of each applicable prot ocol prior to initiating any research with these

seeds.

Research plots must not be located within a 1⁄4 m ile of critical habitats of endangered/threatened lepidopteran and coleopteran species as list ed by the US Fish and Wildlife Service.STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contam inate water, food or feed by storage or disposal. Any seed not used in these experiments must be returned to the seed provider or disposed of as specified in the field protocols. Do not reuse seed containers. En sure that containers are co mpletely empty of seeds before destroying.

## 23) 1507 x MIR162 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERM IT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resis tant corn that produces the following proteins with activity against certain lepidopteran and corn rootworm pests: Cry1F, VIP3A and mCry3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA- approved Experimental Use Program. For use only at an application site of a Part icipant or Coordinator and in accordance with the terms and conditions of the Experimental Use Permit (E UP). This labeling must be in possession of the user at the time the corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry1F protein in DAS-Ø15Ø7-1 and the genetic material necessary for its producti on in corn................................................................<0 .01415%*

Bacillus thuringiensis Vip3Aa20 protein in SYN-IR162-4 and the genetic material necessary for its producti on in co rn.............................................................. <0.0 0403%**

Bacillus thuringiensis mCry3A protein in SYN-IR6Ø4-5 and the genetic material necessary fo r its production in corn...................................................... <0 .000000313 %**

Inert Ingredients:

A marker protein and the genetic material necessary for its production in event D AS-Ø15Ø7-1.............................................................................. .........0.0%*

A marker protein and the genetic material necessary for its production in event SYN-IR6Ø4-5 & SYN-I R162-4................................................... <0 .00019%**

* Maximum percent (wt/wt) of total extractable protein in grain
** Maximum percent (wt/wt) of dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Pioneer Hi-Br ed International, Inc.

7300 NW 62 nd Ave. Johnston, IA 50131 NET CONTENTS: ___________pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may only be used according to the field protocols as specified in the approved EUP program. Participants and Cooperators must have a copy of each applicable prot ocol prior to initiating any research with these

seeds.

Research plots must not be located within a 1⁄4 m ile of critical habitats of endangered/threatened lepidopteran and coleopteran species as list ed by the US Fish and Wildlife Service.STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contam inate water, food or feed by storage or disposal. Any seed not used in these experiments must be returned to the seed provider or disposed of as specified in the field protocols. Do not reuse seed containers. En sure that containers are co mpletely empty of seeds before destroying.

## 24) 59122 x MON810 x MIR162 Corn Seed

EPA EXPERIMENTAL USE PERM IT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resis tant corn that produces the following proteins with activity against certain lepidopteran and corn r ootworm pests: Cry34/35Ab1, Cry1Ab and VIP3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA- approved Experimental Use Program. For use only at an application site of a Part icipant or Coordinator and in accordance with the terms and conditions of the Experimental Use Permit (E UP). This labeling must be in possession of the user at the time the corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry34Ab1 protein in DAS-59122-7 and the genetic material necessa ry for its produc tion in corn.................................................................. <0.0117%*

Bacillus thuringiensis Cry35Ab1 protein in DAS-59122-7 and the genetic material necessary for its producti on in co rn..............................................................<0.0 00161%*

Bacillus thuringiensis Cry1Ab protein in MON-ØØ81Ø-6 and the genetic material necessary for its producti on in corn................................................................. <0.0070%*

Bacillus thuringiensis Vip3Aa20 protein in SYN-IR162-4 and the genetic material necessary for its producti on in corn................................................................<0 .00403%*

Inert Ingredients:

A marker protein and the genetic material necessary for its production in event D AS-59122-7.............................................................................. ...........0.0%*

A marker protein and the genetic material necessary for its producti on in event SYN-IR1 62-4.............................................................................. .<0.00019%*

* Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Pioneer Hi-Br ed International, Inc.

7300 NW 62 nd Ave. Johnston, IA 50131 NET CONTENTS: ___________pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may only be used according to the field protocols as specified in the approved EUP program. Participants and Cooperators must have a copy of each applicable prot ocol prior to initiating any research with these

seeds.

Research plots must not be located within a 1⁄4 m ile of critical habitats of endangered/threatened lepidopteran and coleopteran species as list ed by the US Fish and Wildlife Service.STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contam inate water, food or feed by storage or disposal. Any seed not used in these experiments must be returned to the seed provider or disposed of as specified in the field protocols. Do not reuse seed containers. En sure that containers are co mpletely empty of seeds before destroying.

## 25) 59122 x MON810 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERM IT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resis tant corn that produces the following proteins with activity against certain lepidopteran and corn r ootworm pests: Cry34/35Ab1, Cry1Ab and mCry3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA- approved Experimental Use Program. For use only at an application site of a Part icipant or Coordinator and in accordance with the terms and conditions of the Experimental Use Permit (E UP). This labeling must be in possession of the user at the time the corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry34Ab1 protein in DAS-59122-7 and the genetic material necessa ry for its produc tion in corn.................................................................. <0.0117%*

Bacillus thuringiensis Cry35Ab1 protein in DAS-59122-7 and the genetic material necessary for its producti on in co rn..............................................................<0.0 00161%*

Bacillus thuringiensis Cry1Ab protein in MON-ØØ81Ø-6 and the genetic material necessary for its producti on in corn................................................................. <0.0070%*

Bacillus thuringiensis mCry3A protein in SYN-IR6Ø4-5 and the genetic material necessary fo r its production in corn........................................................<0 .0000003 13%*

Inert Ingredients:

A marker protein and the genetic material necessary for its production in event D AS-59122-7.............................................................................. ...........0.0%*

A marker protein and the genetic material necessary for its producti on in event SYN-IR6 Ø4-5 .............................................................................. <0.00060%*

* Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Pioneer Hi-Br ed International, Inc.

7300 NW 62 nd Ave. Johnston, IA 50131 NET CONTENTS: ___________pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may only be used according to the field protocols as specified in the approved EUP program. Participants and Cooperators must have a copy of each applicable prot ocol prior to initiating any research with these

seeds.

Research plots must not be located within a 1⁄4 m ile of critical habitats of endangered/threatened lepidopteran and coleopteran species as list ed by the US Fish and Wildlife Service.STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contam inate water, food or feed by storage or disposal. Any seed not used in these experiments must be returned to the seed provider or disposed of as specified in the field protocols. Do not reuse seed containers. En sure that containers are co mpletely empty of seeds before destroying.

## 26) 59122 x MIR162 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERM IT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resis tant corn that produces the following proteins with activity against certain lepidopteran and corn r ootworm pests: Cry34/35Ab1, VIP3A and mCry3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA- approved Experimental Use Program. For use only at an application site of a Part icipant or Coordinator and in accordance with the terms and conditions of the Experimental Use Permit (E UP). This labeling must be in possession of the user at the time the corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry34Ab1 protein in DAS-59122-7 and the genetic material necessa ry for its produc tion in corn.................................................................. <0.0117%*

Bacillus thuringiensis Cry35Ab1 protein in DAS-59122-7 and the genetic material necessary for its producti on in co rn..............................................................<0.0 00161%*

Bacillus thuringiensis Vip3Aa20 protein in SYN-IR162-4 and the genetic material necessary for its producti on in corn................................................................<0 .00403%*

Bacillus thuringiensis mCry3A protein in SYN-IR6Ø4-5 and the genetic material necessary fo r its production in corn........................................................<0 .0000003 13%*

Inert Ingredients:

A marker protein and the genetic material necessary for its production in event D AS-59122-7.............................................................................. ...........0.0%*

A marker protein and the genetic material necessary for its production in event SYN-IR6Ø4-5 & SYN-I R162-4.....................................................<0 .00019%*

* Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Pioneer Hi-Br ed International, Inc.

7300 NW 62 nd Ave. Johnston, IA 50131 NET CONTENTS: ___________pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may only be used according to the field protocols as specified in the approved EUP program. Participants and Cooperators must have a copy of each applicable prot ocol prior to initiating any research with these

seeds.

Research plots must not be located within a 1⁄4 m ile of critical habitats of endangered/threatened lepidopteran and coleopteran species as list ed by the US Fish and Wildlife Service.STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contam inate water, food or feed by storage or disposal. Any seed not used in these experiments must be returned to the seed provider or disposed of as specified in the field protocols. Do not reuse seed containers. En sure that containers are co mpletely empty of seeds before destroying.

## 27) MON810 x MIR162 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERM IT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resis tant corn that produces the following proteins with activity against certain lepidopteran and corn rootworm pests: Cry1Ab, VIP3A and mCry3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA- approved Experimental Use Program. For use only at an application site of a Part icipant or Coordinator and in accordance with the terms and conditions of the Experimental Use Permit (E UP). This labeling must be in possession of the user at the time the corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry1Ab protein in MON-ØØ81Ø-6 and the genetic material necessary for its producti on in corn................................................................. <0.0070%*

Bacillus thuringiensis Vip3Aa20 protein in SYN-IR162-4 and the genetic material necessary for its producti on in corn................................................................<0 .00403%*

Bacillus thuringiensis mCry3A protein in SYN-IR6Ø4-5 and the genetic material necessary fo r its production in corn........................................................<0 .0000003 13%*

Inert Ingredient:

A marker protein and the genetic material necessary for its production in event SYN-IR6Ø4-5 & SYN-I R162-4.....................................................<0 .00019%*

* Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Pioneer Hi-Br ed International, Inc.

7300 NW 62 nd Ave. Johnston, IA 50131 NET CONTENTS: ___________pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may only be used according to the field protocols as specified in the approved EUP program. Participants and Cooperators must have a copy of each applicable prot ocol prior to initiating any research with these

seeds.

Research plots must not be located within a 1⁄4 m ile of critical habitats of endangered/threatened lepidopteran and coleopteran species as list ed by the US Fish and Wildlife Service.STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contam inate water, food or feed by storage or disposal. Any seed not used in these experiments must be returned to the seed provider or disposed of as specified in the field protocols. Do not reuse seed containers. En sure that containers are co mpletely empty of seeds before destroying.

## 28) 1507 x MIR162 Corn Seed

EPA EXPERIMENTAL USE PERM IT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resis tant corn that produces the following proteins with activity against certain lepidopteran pests: Cry1F and VIP3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA- approved Experimental Use Program. For use only at an application site of a Part icipant or Coordinator and in accordance with the terms and conditions of the Experimental Use Permit (E UP). This labeling must be in possession of the user at the time the corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry1F protein in DAS-Ø15Ø7-1 and the genetic material necessary for its producti on in corn................................................................<0 .01415%*

Bacillus thuringiensis Vip3Aa20 protein in SYN-IR162-4 and the genetic material necessary for its producti on in co rn.............................................................. <0.0 0403%**

Inert Ingredients:

A marker protein and the genetic material necessary for its production in event D AS-Ø15Ø7-1.............................................................................. < 0.0020%*

A marker protein and the genetic material necessary for its production in event SYN -IR162-4............................................................................. < 0.00019%**

* Maximum percent (wt/wt) of total extractable protein in grain
** Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Pioneer Hi-Br ed International, Inc.

7300 NW 62 nd Ave. Johnston, IA 50131 NET CONTENTS: ___________pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may only be used according to the field protocols as specified in the approved EUP program. Participants and Cooperators must have a copy of each applicable prot ocol prior to initiating any research with these

seeds.

Research plots must not be located within a 1⁄4 m ile of critical habitats of endangered/threatened lepidopteran species as listed by the US Fish and Wildlife Service.STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contam inate water, food or feed by storage or disposal. Any seed not used in these experiments must be returned to the seed provider or disposed of as specified in the field protocols. Do not reuse seed containers. En sure that containers are co mpletely empty of seeds before destroying.

## 29) 1507 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERM IT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resis tant corn that produces the following proteins with activity against certain lepidopteran and corn rootworm pests: Cry1F and mCry3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA- approved Experimental Use Program. For use only at an application site of a Part icipant or Coordinator and in accordance with the terms and conditions of the Experimental Use Permit (E UP). This labeling must be in possession of the user at the time the corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry1F protein in DAS-Ø15Ø7-1 and the genetic material necessary for its producti on in corn................................................................<0 .01415%*

Bacillus thuringiensis mCry3A protein in SYN-IR6Ø4-5 and the genetic material necessary fo r its production in corn..................................................... < 0.000000313 %**

Inert Ingredients:

A marker protein and the genetic material necessary for its production in event D AS-Ø15Ø7-1.............................................................................. .........0.0%*

A marker protein and the genetic material necessary for its production in event SYN -IR6Ø4-5 ............................................................................ <0 .00060%**

* Maximum percent (wt/wt) of total extractable protein in grain
** Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Pioneer Hi-Br ed International, Inc.

7300 NW 62 nd Ave. Johnston, IA 50131 NET CONTENTS: ___________pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may only be used according to the field protocols as specified in the approved EUP program. Participants and Cooperators must have a copy of each applicable prot ocol prior to initiating any research with these

seeds.

Research plots must not be located within a 1⁄4 m ile of critical habitats of endangered/threatened lepidopteran and coleopteran species as list ed by the US Fish and Wildlife Service.STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contam inate water, food or feed by storage or disposal. Any seed not used in these experiments must be returned to the seed provider or disposed of as specified in the field protocols. Do not reuse seed containers. En sure that containers are co mpletely empty of seeds before destroying.

## 30) 59122 x MIR162 Corn Seed

EPA EXPERIMENTAL USE PERM IT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resis tant corn that produces the following proteins with activity against certain lepidopteran and corn rootworm pests: Cry34/35Ab1 and VIP3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA- approved Experimental Use Program. For use only at an application site of a Part icipant or Coordinator and in accordance with the terms and conditions of the Experimental Use Permit (E UP). This labeling must be in possession of the user at the time the corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry34Ab1 protein in DAS-59122-7 and the genetic material necessary for its producti on in corn.................................................................< 0.0117 %*

Bacillus thuringiensis Cry35Ab1 protein in DAS-59122-7 and the genetic material necessary for its producti on in co rn..............................................................<0.0 00161%*

Bacillus thuringiensis Vip3Aa20 protein in SYN-IR162-4 and the genetic material necessary for its producti on in corn................................................................<0 .00403%*

Inert Ingredients:

A marker protein and the genetic material necessary for its production in event D AS-59122-7.............................................................................. ...........0.0%*

A marker protein and the genetic material necessary for its producti on in event SYN-IR1 62-4.............................................................................. .<0.00019%*

* Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Pioneer Hi-Br ed International, Inc.

7300 NW 62 nd Ave. Johnston, IA 50131 NET CONTENTS: ___________pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may only be used according to the field protocols as specified in the approved EUP program. Participants and Cooperators must have a copy of each applicable prot ocol prior to initiating any research with these

seeds.

Research plots must not be located within a 1⁄4 m ile of critical habitats of endangered/threatened lepidopteran and coleopteran species as list ed by the US Fish and Wildlife Service.STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contam inate water, food or feed by storage or disposal. Any seed not used in these experiments must be returned to the seed provider or disposed of as specified in the field protocols. Do not reuse seed containers. En sure that containers are co mpletely empty of seeds before destroying.

## 31) 59122 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERM IT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resis tant corn that produces the following proteins with activity against corn rootworm pe sts: Cry34/35Ab1 and mCry3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA- approved Experimental Use Program. For use only at an application site of a Part icipant or Coordinator and in accordance with the terms and conditions of the Experimental Use Permit (E UP). This labeling must be in possession of the user at the time the corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry34Ab1 protein in DAS-59122-7 and the genetic material necessa ry for its produc tion in corn.................................................................. <0.0117%*

Bacillus thuringiensis Cry35Ab1 protein in DAS-59122-7 and the genetic material necessary for its producti on in co rn..............................................................<0.0 00161%*

Bacillus thuringiensis mCry3A protein in SYN-IR6Ø4-5 and the genetic material necessary fo r its production in corn........................................................<0 .0000003 13%*

Inert Ingredients:

A marker protein and the genetic material necessary for its production in event D AS-59122-7.............................................................................. ...........0.0%*

A marker protein and the genetic material necessary for its producti on in event SYN-IR6 Ø4-5 .............................................................................. <0.00060%*

* Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Pioneer Hi-Br ed International, Inc.

7300 NW 62 nd Ave. Johnston, IA 50131 NET CONTENTS: ___________pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may only be used according to the field protocols as specified in the approved EUP program. Participants and Cooperators must have a copy of each applicable prot ocol prior to initiating any research with these

seeds.

Research plots must not be located within a 1⁄4 m ile of critical habitats of endangered/threatened coleopteran species as listed by t he US Fish and Wildlife Service.STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contam inate water, food or feed by storage or disposal. Any seed not used in these experiments must be returned to the seed provider or disposed of as specified in the field protocols. Do not reuse seed containers. En sure that containers are co mpletely empty of seeds before destroying.

## 32) MON810 x MIR162 Corn Seed

EPA EXPERIMENTAL USE PERM IT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resis tant corn that produces the following proteins with activity against certain lepidopteran pests: Cry1Ab and VIP3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA- approved Experimental Use Program. For use only at an application site of a Part icipant or Coordinator and in accordance with the terms and conditions of the Experimental Use Permit (E UP). This labeling must be in possession of the user at the time the corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry1Ab protein in MON-ØØ81Ø-6 and the genetic material necessary for its producti on in corn................................................................. <0.0070%*

Bacillus thuringiensis Vip3Aa20 protein in SYN-IR162-4 and the genetic material necessary for its producti on in corn................................................................<0 .00403%*

Inert Ingredient:

A marker protein and the genetic material necessary for its producti on in event SYN-IR1 62-4.............................................................................. .<0.00019%*

* Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Pioneer Hi-Br ed International, Inc.

7300 NW 62 nd Ave. Johnston, IA 50131 NET CONTENTS: ___________pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may only be used according to the field protocols as specified in the approved EUP program. Participants and Cooperators must have a copy of each applicable prot ocol prior to initiating any research with these

seeds.

Research plots must not be located within a 1⁄4 m ile of critical habitats of endangered/threatened lepidopteran species as listed by the US Fish and Wildlife Service.

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contam inate water, food or feed by storage or disposal. Any seed not used in these experiments must be returned to the seed provider or disposed of as specified in the field protocols. Do not reuse seed containers. En sure that containers are co mpletely empty of seeds before destroying.

## 33) MON810 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERM IT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resis tant corn that produces the following proteins with activity against certain lepidopteran and corn rootworm pests: Cry1Ab and mCry3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA- approved Experimental Use Program. For use only at an application site of a Part icipant or Coordinator and in accordance with the terms and conditions of the Experimental Use Permit (E UP). This labeling must be in possession of the user at the time the corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry1Ab protein in MON-ØØ81Ø-6 and the genetic material (PV-ZMBK07) necessa ry for its production in corn ..........................................<0. 0070%*

Bacillus thuringiensis mCry3A protein in SYN-IR6Ø4-5 and the genetic material necessary fo r its production in corn........................................................<0 .0000003 13%*

Inert Ingredient:

A marker protein and the genetic material necessary for its producti on in event SYN-IR6 Ø4-5 .............................................................................. <0.00060%*

* Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Pioneer Hi-Br ed International, Inc.

7300 NW 62 nd Ave. Johnston, IA 50131 NET CONTENTS: ___________pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may only be used according to the field protocols as specified in the approved EUP program. Participants and Cooperators must have a copy of each applicable prot ocol prior to initiating any research with these

seeds.

Research plots must not be located within a 1⁄4 m ile of critical habitats of endangered/threatened lepidopteran and coleopteran species as list ed by the US Fish and Wildlife Service.

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contam inate water, food or feed by storage or disposal. Any seed not used in these experiments must be returned to the seed provider or disposed of as specified in the field protocols. Do not reuse seed containers. En sure that containers are co mpletely empty of seeds before destroying.

## 34) MIR162 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERM IT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resis tant corn that produces the following proteins with activity against certain lepidopteran and co rn rootworm pests: VIP3A and mCry3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA- approved Experimental Use Program. For use only at an application site of a Part icipant or Coordinator and in accordance with the terms and conditions of the Experimental Use Permit (E UP). This labeling must be in possession of the user at the time the corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Vip3Aa20 protein in SYN-IR162-4 and the genetic material necessary for its producti on in corn................................................................<0 .00403%*

Bacillus thuringiensis mCry3A protein in SYN-IR6Ø4-5 and the genetic material necessary fo r its production in corn........................................................<0 .0000003 13%*

Inert Ingredient:

A marker protein and the genetic material necessary for its production in event SYN-IR6Ø4-5 & SYN-I R162-4.....................................................<0 .00019%*

* Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Pioneer Hi-Br ed International, Inc.

7300 NW 62 nd Ave. Johnston, IA 50131 NET CONTENTS: ___________pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may only be used according to the field protocols as specified in the approved EUP program. Participants and Cooperators must have a copy of each applicable prot ocol prior to initiating any research with these

seeds.

Research plots must not be located within a 1⁄4 m ile of critical habitats of endangered/threatened lepidopteran and coleopteran species as list ed by the US Fish and Wildlife Service.

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contam inate water, food or feed by storage or disposal. Any seed not used in these experiments must be returned to the seed provider or disposed of as specified in the field protocols. Do not reuse seed containers. En sure that containers are co mpletely empty of seeds before destroying.PHI-2008-139 Page 190 of 237 August 14, 2008

Section C. Reference to Available Environmental Toxicity Data

Summary of data for individual events

59122, 1507, and MON 810 ar e each registered even ts; extensive data have been submitted to EPA and have been previously review ed (U.S. EPA, 2001 & 2005). To su mmarize, nontarget organism testing was performed on each of the single events (o r their respective Bt proteins) with honeybees (larvae and adults), Daphnia magna , lady beetles, parasitic hy menoptera, green lacewing, earthworms, and collembolans. No adverse effects were found at environmentally relevant exposures for any of the species tested. As one of the most publicly recognized insects, monarch butterfly larvae were also tested for sensitivity to Cry1F and Cry1 Ab, and no significant risk to monarchs was found at environmentally relevant exposures. Additionally , environmental fate data indicate that Cry1F, Cry34/35Ab1 and Cry1Ab dissipate rapidly from soil. Therefore accumulation of the proteins in soil should be minimal, thereby decreasing the likelih ood of exposure to nontarget soil organisms. The Agency previously concluded that the levels of Cry 1F, Cry 1Ab, and Cry 34/35Ab1 likely to occur in the environment will not po se unreasonable risk to nontarget organisms, including mammals, birds, fish, bees, and other invertebrates, based on the required and voluntary data submitted.

Specific data for proposed stack events

Pioneer measured the concentrations of Cry1F, Cry3 4/35Ab1, Cry1Ab and PAT in plant tissues of the proposed stacked events. Expres sion levels were similar to t hose of the single events (PHI-2008- 006), thereby indicating no increase in potential exposure concentrations compared to the previously

reviewed individual events.

Pioneer also evaluated the effects of pollen from t he proposed stacked events on monarch butterfly larvae. No adverse effects were seen (PHI-2008- 104 and PHI-2008-105); therefore, we do not expect any additional risk from the stacked products com pared to single events for non-target lepidoptera.

In addition, Pioneer confirmed that the efficacy of 1507 pyramided with MON810 is additive in nature (as opposed to synergistic or antagonistic) agains t European corn borer, southwestern corn borer, corn earworm, and fall armyworm (PHI-2008-014). Because there was no evidence of synergy during testing of several key target pests, we do not expect synergy to occur in non-target insects.

Based on the lack of environmental and mammalian effe cts in the individual events, the similarity in expression levels, the lack of synergy seen in additivi ty testing, and the low likelihood of exposure, we expect no adverse effects from the proposed stacke d products to mammals, birds, fish, bees, other invertebrates, and plants. Lack of exposure due to the small scale of testing plots and the limited duration of the EUP also points to a very low lik elihood of risk from the proposed stacked product, similar to the findings by EPA for the previously registered individual events. In addition, the EUP Program will be conducted on land that is currently in agricultural us e. Therefore, we do not expect that the proposed stacked produc t, which may include all of these proteins, will have a different environmental profile than the i ndividual products that have bee n previously reviewed by EPA.

References

U.S. EPA. 2001. Environmental Assessment: Bt Plant-Incorporated Protectants. Biopesticides Registration Action Document. October 15, 2001. Accessed June 24, 2008: http://www.epa.gov/oppbppd1/biopesticides/pips/bt_brad2/3-ecological.pdf

U.S. EPA. 2005. Environmental Assessment: Bacillus thuringiensis Cry34/35Ab1 Corn Biopesticide Registration Action Document. October 2005. Accessed June 24, 2008: http://www.epa.gov/pesticides/biopesticides/ingredients/tech_docs/brad_006490.pdfPHI-2008-139 Page 191 of 237 August 14, 2008

Section D. Residue Data

The Cry1F, Cry1Ab and Cry34/35 proteins and the ge netic material necessary for their production in corn are plant-incorporated protec tants (PIPs). These PIPs are not applied to the corn crop as conventional pesticides would be, but rather are synthesized by plants carrying the cry1f

, Cry1Ab and cry34/35 genes. Residue data per se are not available for a PIP. However, for purposes of assessing dietary and environmental risk of 1507x59122x MON810, 1507xMON810 and 59122XMON810, the concentrations of Cry1F, Cry1(A)b, Cry34/35 and ph osphinothricin acetyltransferase (PAT) proteins were determined in different plant tissues at diffe rent plant growth stages using quantitative ELISA assays. The results from this determination are summarized in Volume 5 of this submission (PHI2008-006).PHI-2008-139 Page 192 of 237 August 14, 2008

Section E. Effectiveness Data

To date, limited efficacy data have been generated on the EUP breeding stacks listed in Table 1. However, the individual events are well characteri zed with respect to efficacy, and 1507 and MON810 have been demonstrated to be additive in nature, as opposed to synergistic or antagonistic (PHI-2008- 114). Therefore, the breeding pyramids of 1507xMON810 and 1507x59122xMON810 are expected to be efficacious against the pests listed in Table 2.

Table 2. Lepidopteran Pests That Are Controlled or Suppressed by Pyramided 1507xMON810*

Scientific name Common name 1507 Activity MON810 Activity Predicted

1507 x MON810 Activity

Agrotis ipsilon Black cutworm Yes No Yes

Helicoverpa zea Corn earworm Yes Yes Yes

Ostrinia nubilalis European corn borer Yes Yes Yes

Spodoptera frugiperda Fall armyworm Yes Yes Yes

Diatraea grandiosella Southwestern corn

borer Yes Yes Yes

Richia albicosta Western bean cutworm Yes No Yes

Elasmopalpus lignosellus Lesser corn stalk borer Yes No Yes

Diatrae crambidoides Southern corn stalk

borer Yes Yes Yes

Diatrea saccharalis Sugarcane borer Yes No Yes

Papaipema nebris Common stalk borer No Yes Yes

* based on current labels

The breeding stacks of 1507x59122xMON810 and 59122xMON810 are expected to be equal in efficacy to the 59122 event alone against corn rootworm pests: Diabrotica virgifera virgifera, western corn rootworm (WCRW), Diabrotica berberi , northern corn rootworm (NCRW), and Diabrotica virgiferea zeae , Mexican corn rootworm (MCRW).

Under the proposed EUP program, efficacy against lepidopteran and coleopteran pests will be evaluated in a range of environments, gr owing conditions and genetic backgrounds.

Thus far, limited field efficacy trials have been comp leted characterizing first generation European corn borer, Ostrinia nubilalis (Hubner) (ECB), foliar feeding and fall armyworm, Spodoptera frugiperda (J.E. Smith) (FAW), whorl feeding on three maiz e hybrids containing events 1507x59122, 1507x59122xMON810, and a negative control (isolin e). The experimental design included ninePHI-2008-139 Page 193 of 237 August 14, 2008

entries (each a single-row plot with 30” row spacin g) arranged in a randomized complete block with three replications.

For trials characterizing first generation ECB feedi ng, 100 neonates/plant were manually infested three times within a 7-day period (300 total neonates /plant) beginning when plants reached growth stage V5. Approximately three weeks after the last infe station, mean damage ratings were taken on either 10 or 15 individual plants per plot, depending on location (Table 3).

For trials characterizing fall armyworm feeding, 20 neonates/plant were manually infested three times within a 10-day period (60 total neonates/plant) beginning when plants reached growth stage V4. Approximately two weeks after the last infestati on, mean damage ratings were taken on either 10 or 15 plants per plot, depending on location (Table 4).

Results to date indicate that early vegetativ e-stage maize hybrids co ntaining either events 1507x59122xMON810 or events 1507x59122 were nearly immune to first generation ECB and FAW foliar injury. Additionally, there were no significant differences in mean damage ratings between hybrids containing events 1507x59122xMON810 and hy brids with only 1507x59122 for both pests at

all locations.

These results demonstrate the efficacy of these ev ents is stable regardless of stacking or pyramiding

in the event combinations named in Table 1. The EU P will allow for more extens ive efficacy testing to further characterize the pest spectrum and level of efficacy of named event combinations under a wider range of environmental conditions.PHI-2008-139 Page 194 of 237 August 14, 2008

Table 3. Efficacy of Breeding Stacks Against First Generation European Corn Borer

Mean damage ratings characterizing first generation ECB foliar feeding on maize hybrids containing events 1507x59122, 1507x59122xMON810, or a negative c ontrol (isoline) at 5 field trial locations in

2008.

Location Event Combination n a Mean Damage Rating 
Standard Error b,c

1507x59122 135 9.0  0.00 a

1507x59122xMON810 135 9.0  0.00 a York, NE

Negative control 135 5.4  0.07 b

1507x59122 135 9.0  0.00 a

1507x59122xMON810 135 9.0  0.00 a Dallas Center, IA

Negative control 135 5.1  0.07 b

1507x59122 90 9.0  0.00 a

1507x59122xMON810 90 9.0  0.00 a Johnston, IA

Negative control 89 5.6  0.07b

1507x59122 135 9.0  0.00 a

1507x59122xMON810 135 9.0  0.00 a Mankato, MN

Negative control 135 5.8  0.09 b

1507x59122 135 9.0  0.00 a

1507x59122xMON810 132 9.0  0.00 a Princeton, IL

Negative control 135 6.0  0.08 b

a n = number of plants characterize d; 3 maize hybrid backgrounds, 3 replications, and either 10 or 15 plants/plot depending on location.

b Damage ratings on individual plants were determined using the following visual rating scale: 9. No visible leaf injury or a small amount of pin or fine shot-hole type injury on a few leaves. 8. Small amount of shot-hole type lesions on a few leaves. 7. Shot-hole injury common on several leaves. 6. Several leaves with shot-hole and elongated lesions (Lesions <0.5” in length). 5. Several leaves with elongated lesions (Lesions 0.5” to 1.0” in length). 4. Several leaves with elongated lesions (Lesions >1.0” in length). 3. Long lesions (>1.0”) common on about one-half the leaves. 2. Long lesions (>1.0”) common on about two-thirds the leaves. 1. Most of the leaves with long lesions.

c Within a location, means with the same letter are not significantly different (Fisher’s Protected LSD

test, P > 0.05).PHI-2008-139 Page 195 of 237 August 14, 2008

Table 4. Efficacy of Breeding Stacks Against Fall Armyworm

Mean damage ratings characterizing FAW fo liar feeding on maize hybrids containing events 1507x59122, 1507x59122xMON810, or a negative control (iso line) at 2 field trial locations in 2008.

Location Event Combination n a Mean Damage Rating 
Standard Error b,c

1507x59122 135 8.9  0.02 a

1507x59122xMON810 135 8.9  0.02 a Dallas Center, IA

Negative control 135 2.4  0.07 b

1507x59122 90 9.0  0.00 a

1507x59122xMON810 89 9.0  0.02 a Johnston, IA

Negative control 89 2.8  0.09 b

a n = number of plants characterize d; 3 maize hybrid backgrounds, 3 replications, and either 10 or 15 plants/plot depending on location.

b Damage ratings on individual plants were determined using the following visual rating scale: 9. No damage to pinhole lesions present on whorl leaves. 8. Pinholes and small circular lesions present on whorl leaves.

7. Small circular lesions and a few small el ongated (rectangular shaped) lesions up to 0.5” in length present on whorl and furl leaves. 6. Several small elongated lesions 0.5” to 1” in length on a few whorl and furl leaves. 5. Several large elongated lesions greater than 1” in length present on a few whorl and furl leaves

and/or a few small to mid-sized uniform to irregular shaped holes (basement membrane consumed) in whorl and furl leaves. 4. Several large elongated lesions present on seve ral whorl and furl leaves and/or several large uniform to irregular shaped holes in whorl and furl leaves. 3. Many elongated lesions of all sizes present on several whorl leaves plus several large uniform to irregular shaped holes in whorl and furl leaves. 2. Many elongated lesions of all sizes present on most whorl and furl leaves plus many mid to large-sized uniform to irregular shaped holes in whorl and furl leaves. 1. Whorl and furl leaves almost totally destroyed.

c Within a location, means with the same letter are not significantly different (Fisher’s Protected LSD

test, P > 0.05).PHI-2008-139 Page 196 of 237 August 14, 2008

Section F. Tolerance if Testing Involves Use in Food or Feed

Phosphinothricin acetyltransferase (PAT), the mark er protein in 1507 and 59122 corn, is exempt from the requirement of a tolerance in all plants: 40CFR §180.1151.

MON810 does not contain a marker protein.

Cry1Ab, the PIP in MON810 corn, is exempt from the requirement of a tolerance in all plants: 40CFR §180.1173.

Cry1F, the PIP in 1507 corn, is exempt from the r equirement of a tolerance in corn: 40CFR §180.1217.

Cry34Ab1 and Cry35Ab1, the PIPs in 59122 corn, are exempt from the requirement of a tolerance in corn: 40CFR §174.457.Section G. Proposed Experimental Program

For the adequate development of insect-protected transgenic plants, it is necessary to properly characterize the performance of the crop through larg e-scale field testing. In order to thoroughly evaluate corn plants, Experiments must be grown at a large number of locations, with many replications of each experiment, in order to obtain sufficient data for hybrid evaluation and advancement. Placement of Experiments with rese arch centers and cooper ators is typically not decided until early spring of any given year. Spring weather conditions can affect planting locations. Therefore, the proposed EU P acreage needs to take into accoun t the possibility of Experiments being planted in a variety of locations and states. Beca use of this flexibility needed, the proposed acreage is likely an overestimate of the amount of EUP material that will actually be planted.

G1. Participants in the Experimental Program

Table 5 contains a list of the Participants who will supervise the work conducted under this EUP. All Participants are employed by Dupont/Pioneer Hi-Bred International, Inc. Participants listed below may supervise EUP Blocks in states othe r than their own (for example, if they have a cooperator across the state line).

Table 5. List of Participants

State Participant Name Participant Address Phone

Number

AR Mark Hood and/or

Bob Suttner Pioneer Hi-Bred International Inc., 11329 Highway 70, P.O. BOX 309, Proctor, AR 72376 870-702-7180

CA Eduardo Traut and/or

Salim Hakimi Pioneer Hi-Bred International Inc., 18285 County Road 96, Woodland, CA 95695 530-666-1084

CO Chris Fagan and/or Don Pope Pioneer Hi-Bred International Inc., 4200 West Service Road, Unit #4, Evans, CO 8062 970-506-9219

DE Mark Williams and/or Dan Mongeau DuPont Experimental Station, Route 141, Henry Clay Bldg #353, Wilmington, DE 19880 302-695-8858

GA Dan Gorman and/or

Joshua Stanaland Pioneer Hi-Bred International Inc., 2300 Industrial Park Road NE, Cairo, GA 39828 229-378-8240

HI Bruce Heckman

and/or Rhian Campbell Pioneer Hi-Bred International Inc., 1-385 Kaumaualii Highway, Waimea, Kauai, HI 96796 808-338-8300

HI Steven Kai and/or Jill Suga Pioneer Hi-Bred International Inc., 7431 Kaumaualii Highway, P.O. Box 8, Kekaha, HI 96752 808-337-1413

HI Richard McCormack and/or Alika Napier Pioneer Hi-Bred International Inc., 67-172 Farrington Highway, P.O. Box 520, Waialua, HI

96791 808-637-0100

IA Joseph Gogerty

and/or Marcus Marine Pioneer Hi-Bred International Inc., 1920 E McGregor Road, P.O. Box 557, Algona, IA 50511 515-295-7283

IA Delos Borcherding and/or Kerry Mounsey Pioneer Hi-Bred International Inc., 810 Sugar Grove Avenue, Dallas Center, IA 50063 515-270-4259

IA Kurt Hohnstrater

and/or Bob Bonewitz Pioneer Hi-Bred International Inc., 7300 NW 62nd Avenue, P.O. Box 1004, Johnston, IA 50131 515-334-4575IA Jeff Schussler and/or Ryan Pape Pioneer Hi-Bred International Inc., 3261 N. Alburnett Road, P.O. Box 428, Marion, IA 52302 319-377-2870

IL Don Westerhof and/or

David Hobbs Pioneer Hi-Bred International Inc., 985 County
Road 300 East, Ivesdale, IL 61851 271-564-2339

IL Steve Dofing and/or

Bruce Eidson Pioneer Hi-Bred International Inc., 21888 N 950 th

Road, Adair, IL 61411 309-653-2401

IL Terry Williams and/or

Chris Saacke Pioneer Hi-Bred International Inc., 19741 Illinois Highway 26, Princeton, IL 61356 815-875-6523

IL Larry Hageman and/or

Scott Swanson Dupont Research Farm, 13239 Route 38, Rochelle,

IL 61068 815-562-7570

IL Eric Luce and/or

Trenton Borth Pioneer Hi-Bred International Inc., 3025 East 8th Road, Uttica, IL 61373 815-667-5133

IN Brian Bush and/or

Kevin Kuhn Pioneer Hi-Bred International Inc.,3850 North 100 East, Windfall, IN 46076 765-945-8217

IN Dan Pohl and/or JB

Crow Pioneer Hi-Bred International Inc., 1193 W 400 S,
Princeton, IN 47670 812-385-2935

KS Travis Lee and/or Randy Sutterfield Pioneer Hi-Bred International Inc., 150 Air Links Drive, Garden City, KS 67846 620-276-8149

MI Dina Severns and/or

Bart Hubbell Pioneer Hi-Bred International Inc., 1635 N. Wisner Road, Ithaca, MI 48847 989-875-2220

MN Steve Hauf and/or Cody Nelson Pioneer Hi-Bred International Inc., 4050 30 th

Avenue South, Moorehead, MN 56560 218-299-8610

MN Doug Sublet and/or

Brian Merten Pioneer Hi-Bred International Inc., 19456 State Highway 22, Mankato, MN 56001 507-625-3252

MN Ryan Lee and/or Dan

Kleven Pioneer Hi-Bred International Inc., 1740 45 th Street SE, Willmar, MN 56201 320-235-2422

MO Gary Henke and/or

Chris Zwiener Pioneer Hi-Bred International Inc., Route 1 Box 49A, Miami, MO 65344 660-595-2242

NE Dennis Dolan and/or Gregory Halstead Pioneer Hi-Bred International Inc., 1501 Road P, York, NE 68467 402-362-6639

PA Dave Whitaker and/or

Rick Blum Pioneer Hi-Bred International Inc., 982 New Holland Road, New Holland, PA 17557 717-354-6044

PR Alberio Molina and/orLorimar FigueroaPerez Pioneer Hi-Bred Puerto Rico, Road. #3, KM. 154.9, Salinas, PR 00751 787-824-4440

SD Tim Larson and/or

Daniel Hancock Pioneer Hi-Bred International Inc., 214 West 2 nd St. South, Brookings, SD 57006 605-692-5966

TN Russ Henry and/or Gray Hooks Pioneer Hi-Bred International Inc., 3092 Flippen Lane, Union City, TN 38261 731-885-4882

TX Sharon Rogers and/or Jorge Guzman Pioneer Hi-Bred International, Inc., 1702 South
International Blvd., Weslaco, TX 78596 956-969-1569WA Brett Iverson and/or Cindy Rochleau Pioneer Hi-Bred International Inc., 1040 Settler Road, Connell, WA 99326 509-234-9046

WI Mike O’Leary and/or

John Kinnard Pioneer Hi-Bred International Inc., S5380 Cemetery Road, Eau Claire, WI 54701 715-834-0061

WI Tom Kevern and/or Ryan Temperly Pioneer Hi-Bred International Inc., 1039 South Milton-Shopiere, P.O. Box 668, Janesville, WI

53547 608-756-4030

In addition to the above participants/states, the Participants listed below may have EUP Experiments with cooperators in any of the listed st ates and territories in the EUP Program.

Participant Participant’s Address Phone

Number

Tim Nowatzki Pioneer Hi-Bred International Inc., 7300 NW 62nd Avenue, P.O. Box 1004, Johnston, IA 50131 515-270-4270

Mel Peters Pioneer Hi-Bred International Inc., 7300 NW 62nd Avenue, P.O. Box
1004, Johnston, IA 50131 515-253-2156

Lee Prochaska Pioneer Hi-Bred International Inc., 7250 NW 62 nd Avenue, P.O. Box 552, Johnston, IA 50131 515-334-6703

Paula Davis Pioneer Hi-Bred International Inc., 7100 NW 62nd Avenue, P.O. Box
1000, Johnston, IA 50131 515-334-6994

Jeff Mathesius Pioneer Hi-Bred International Inc., 7100 NW 70 th Avenue, P.O. Box 183, Johnston, IA 50131 515-334-3603

Mike Elsing Pioneer Hi-Bred International Inc., 7300 NW 62nd Avenue, P.O. Box
1004, Johnston, IA 50131 515-253-2132

Glen Umbaugh Pioneer Hi-Bred International Inc., 6900 NW 62nd Avenue, P.O. Box 256, Johnston, IA 50131 515-270-3426

Wayne Mehre Pioneer Hi-Bred International Inc., 7300 NW 62nd Avenue, P.O. Box
1004, Johnston, IA 50131 515-253-2405

Brian Baumhover Pioneer Hi-Bred International Inc., 7301 NW 62nd Avenue, P.O. Box 85, Johnston, IA 50131 515-270-5902

Doug Nubel Pioneer Hi-Bred International Inc., 7300 NW 62nd Avenue, P.O. Box
1004, Johnston, IA 50131 515-253-5780

Russ Fox Pioneer Hi-Bred International Inc., 810 Sugar Grove Avenue, Dallas Center, IA 500631 515-270-4088

Carla Peterman Pioneer Hi-Bred International Inc., 7300 NW 62nd Avenue, P.O. Box
1004, Johnston, IA 50131 515-270-5992

Lindsey Flexner DuPont Experimental Station, Route 141, Henry Clay Bldg #353, Wilmington, DE 19880 302-695-6822

Rachel Binning Pioneer Hi-Bred International Inc., 7250 NW 62nd Avenue, P.O. Box
552, Johnston, IA 50131 515-254-2742

Murt McLeod Pioneer Hi-Bred International Inc., 3850 North 100 East, Windfall, IN

46076 765-945-8217G2. Acreage and Seed Quantities by State or Territory for the EUP Program

Pioneer has endeavored to forecast its acreage needs and field testing program for 2009. The quantity of acres being requested in this application is requ ired for product development and testing. As recommended in EPA’s “Tips for Plant-Incorporated Protecta nt (PIP) Experimenta l Use Permit (EUP) Program Submission” and in guidance provided in the February 6, 2009 letter from BPPD to Pioneer, this acreage calculation includes the following six categories:

 Category 1: EUP Test Plants;

 Category 2: Registered PIPs for breeding or comparison purposes;

 Category 3: Non-registered PIPs;

 Category 4: Registered PIPs (other than thos e listed in Category #2) for comparison purposes only ( i.e ., “commercial checks”);

 Non-PIP plants (for example, herbicide tolerant or non-transgenic); and

 Border or filler rows.

Category 4 includes those commer cial products that will be used fo r non-experimental purposes only (i.e., not for breeding)—for example, c ontrol checks in a yield evaluation.

Table 6 lists the breeding stacks included in catego ries 1, 2 and 3. Each of the individual events listed is registered by EPA. All PIP prot eins have full tolerance exemptions.

Table 6. Breeding Stacks Included in Categories 1, 2 and 3

Category 1

EUP Test Plants Category 2
Registered PIPs for breeding

or comparison purposes Category 3
Non-registered PIPs

1507 x 59122 x MON810 1507 1507 x 59122 x MON810 x MIR162

1507 x MON810 59122 1507 x 59122 x MON810 x MIR604

59122 x MON810 MON810 1507 x 59 122 x MIR162 x MIR604

1507 x 59122 1507 x MON810 x MIR162 x MIR604

MIR604 59122 x MON810 x MIR162 x MIR604

Bt11 1507 x 59122 x MIR162

Bt11 x MIR604 1507 x 59122 x MIR604

MIR162 1507 x MON810 x MIR162

Bt11 x MIR162 1507 x MON810 x MIR604

Bt11 x MIR162 x MIR604 1507 x MIR162 x MIR604

59122 x MON810 x MIR162

591122 x MON810 x MIR604

59122 x MIR162 x MIR604

MON810 x MIR162 x MIR604

1507 x MIR162

1507 x MIR604

59122 x MIR162

59122 x MIR604

MON810 x MIR162

MON810 x MIR604

MIR162 x MIR604

Many of the breeding stacks listed in Category 3 w ill likely not be planted to any appreciable extent. However, in order to enable full flexibility in the breeding and testing program, many potential

combinations are listed.

Table 7 provides the maximum acreage per state. This acreage will not be exceeded.The allocation of these acres between event combinations and protocols (Tables 8-13) represents

best estimates as of this date and should not be used for inspection purposes. Environmental conditions, for example, weather events, pest infe station levels, unforeseen regulatory data demands received from other countries, and other factors, may necessitate a reallocation of acres between protocols and traits. For event combinations where less than an acre may be grown, “<1” has been entered in Tables 8-13 and is counted as zero acres for the purpose of estimating acres by event combinations and protocol (as per BPPD guidance).

Table 14 summarizes the amount of seed to be planted and the total estimated amount of active ingredient to be used in the EUP Program. In th is table, if the estima ted acreage of an event combination is <1, we have assume that 1 acre will be grown (as per BPPD guidance). This has been

done to estimate the maximum amount of a.i. that will be used per state.Table 7. Proposed Total Acreage by State

State PIP PIPs for
comparison
only* Non-PIP Border Total

AR 37 4 6 27 74

CA 31 14 8 28 81

CO 20 5 4 18 47

DE 10 0 2 13 25

GA 41 6 6 24 77

HI 765 1 106 12 884

IA 99 31 24 99 253

IL 679 16 113 51 859

IN 55 14 11 42 122

KS 36 4 6 27 73

KY 7 0 14 12

LA 15 0 2 8 25

MI 18 3 3 13 37

MN 41 6 7 33 87

MO 32 5 6 27 70

MS 16 2 2 8 28

NC 15 0 2 8 25

NE 52 14 12 48 126

NY 7 0 17 15

OH 10 0 2 13 25

OK 15 0 2 6 23

PA 22 4 4 23 53

PR 56 1 6 12 75

SD 30 4 5 25 64

TN 29 5 5 26 65

TX 38 5 7 29 79

WA 660 0 100 0 760

WI 31 6 5 20 62

totals 2867 150 458 651 4126

* This acreage is for registered PIPs that are used for comparison purposes only and are not used for breeding. AcresTable 8. Estimated Acreage per State/Territory for Nursery / Breeding Protocol*

State 1507x59122xMON810 1507xMON810 59122xMON810 Category 1 Total 1507 59122 MON810 1507x59122 MIR604 Bt11 Bt11xMIR604 MIR162 Bt11xMIR162 Bt11xMIR162xMIR604 Category 2 Total 1507x59122xMON810xMIR162 1507x59122xMON810xMIR604 1507x59122xMIR162xMIR604 1507xMON810xMIR162xMIR604 59122xMON810xMIR162xMIR604 1507x59122xMIR162 1507x59122xMIR604 1507xMON810xMIR162 1507xMON810xMIR604 1507xMIR162xMIR604 59122xMON810xMIR162 59122xMON810xMIR604 59122xMIR162xMIR604 MON810xMIR162xMIR604 1507xMIR162 1507xMIR604 59122xMIR162 59122xMIR604 MON810xMIR162 MON810xMIR604 MIR162xMIR604 Category 3 Total Category 4** Total PIP Non-PIP Border Total NON-PIP TotalGA 3 3 410<1 <1 <1 2 <1 <1 <1 <1 <1 <13< 1 < 1 < 1 < 1 < 1 < 1 < 1 < 1 < 1 < 1 < 1 < 1 < 1 < 1 <1 < 1 < 1 < 1 < 1 < 1 < 11 0 141 23 17HI 10 10 1030443511111122< 1 < 1 < 1 < 1 < 1 < 1 < 1 < 1 < 1 < 1 < 1 < 1 < 1 < 1 <1 < 1 < 1 < 1 < 1 < 1 < 11 0 535 914 67PR7 8 1126443511111122< 1 < 1 < 1 < 1 < 1 < 1 < 1 < 1 < 1 < 1 < 1 < 1 < 1 < 1 <1 < 1 < 1 < 1 < 1 < 1 < 11 0 495 914 63total66473 0 11631 147** Category 4 is non-experimental material that will be used for check plots only (not breeding).* The allocation of these acres among various event combinations represents best estimates as of this date and should not be used for inspection purposes. Environmental conditions (e.g.weather events, pest infestation levels), unforeseen regulatory datademands received from other countries, and other factors, may necessitate a reallocation of acres between protocols and traits. For event combinations where less than an acre may be grown, “<1” has been entered and the cumlative total of the "<1" acres iscounted as one acre for the purpose of estimating acres within a Category across event combinations and protocol (as per BPPD guidance).Category 5Category 3Category 1 Category 2Table 9. Estimated Acreage per State/Territory for Yield / Agronomic Protocol*

State 1507x59122xMON810 1507xMON810 59122xMON810 Category 1 Total 1507 59122 MON810 1507x59122 MIR604 Bt11 Bt11xMIR604 MIR162 Bt11xMIR162 Bt11xMIR162xMIR604 Category 2 Total 1507x59122xMON810xMIR162 1507x59122xMON810xMIR604 1507x59122xMIR162xMIR604 1507xMON810xMIR162xMIR604 59122xMON810xMIR162xMIR604 1507x59122xMIR162 1507x59122xMIR604 1507xMON810xMIR162 1507xMON810xMIR604 1507xMIR162xMIR604 59122xMON810xMIR162 59122xMON810xMIR604 59122xMIR162xMIR604 MON810xMIR162xMIR604 1507xMIR162 1507xMIR604 59122xMIR162 59122xMIR604 MON810xMIR162 MON810xMIR604 MIR162xMIR604 Category 3 Total Category 4** Total PIP Non-PIP Border Total NON-PIP TotalAR 3 1 151 <1 <1 1 <1 <1 <1 <1 <1 <13<1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <12 3 132 810 23CA 11 3 1152 2 1 8 <1 <1 <1 <1 <1 <114<1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <12 14 458 2836 81CO 4 1 161 1 <1 2 <1 <1 <1 <1 <1 <15<1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <12 5 183 1114 32GA 4 1 161 1 <1 2 <1 <1 <1 <1 <1 <15<1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <12 5 183 1215 33IA 22 8 13110 5 2 12 <1 <1 <1 <1 <1 <130<1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <12 30 9317 6380 173IL 11 4 1163 3 <1 8 <1 <1 <1 <1 <1 <115<1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <12 15 488 2937 85IN 11 2 1142 2 <1 9 <1 <1 <1 <1 <1 <114<1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <12 13 437 2431 74KS 3 1 151 <1 <1 2 <1 <1 <1 <1 <1 <14<1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <12 3 142 911 25MI 3 1 151 <1 <1 2 <1 <1 <1 <1 <1 <14<1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <12 3 142 911 25MN 4 1 161 <1 <1 4 <1 <1 <1 <1 <1 <16<1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <12 5 193 1215 34MO 4 1 161 <1 <1 4 <1 <1 <1 <1 <1 <16<1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <12 5 193 1215 34MS 2 1 14<1 <1 <1 2 <1 <1 <1 <1 <1 <13<1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <12 2 111 45 16NE 12 2 1152 2 1 9 <1 <1 <1 <1 <1 <115<1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <12 14 468 2836 82PA 3 1 151 <1 <1 3 <1 <1 <1 <1 <1 <15<1<1<1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <12 4 162 1012 28SD 3 1 151 <1 <1 3 <1 <1 <1 <1 <1 <15<1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <12 4 162 1012 28TN 3 1 151 <1 <1 3 <1 <1 <1 <1 <1 <15<1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <12 4 162 1012 28TX 3 3 171 1 1 3 <1 <1 <1 <1 <1 <17<1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <12 4 203 1316 36WI51171 1 <1 4 <1 <1 <1 <1 <1 <17<1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <12 6 223 1316 38total16315336 139 491384 875** Category 4 is non-experimental material that will be used for check plots only (not breeding).* The allocation of these acres among various event combinations represents best estimates as of this date and should not be used for inspection purposes. Environmental conditions (e.g. weather events, pest infestation levels), unforeseen regulatory datademands received from other countries, and other factors, may necessitate a reallocation of acres between protocols and traits. For event combinations where less than an acre may be grown, “<1” has been entered and the cumlative total of the "<1" acresis counted as one acre for the purpose of estimating acres within a Category across event combinations and protocol (as per BPPD guidance).Category 5Category 3Category 1Category 2Table 10. Estimated Acreage per State/Territory for Efficacy / IRM Protocol*

State 1507x59122xMON810 1507xMON810 59122xMON810 Category 1 Total 1507 59122 MON810 1507x59122 MIR604 Bt11 Bt11xMIR604 MIR162 Bt11xMIR162 Bt11xMIR162xMIR604 Category 2 Total 1507x59122xMON810xMIR162 1507x59122xMON810xMIR604 1507x59122xMIR162xMIR604 1507xMON810xMIR162xMIR604 59122xMON810xMIR162xMIR604 1507x59122xMIR162 1507x59122xMIR604 1507xMON810xMIR162 1507xMON810xMIR604 1507xMIR162xMIR604 59122xMON810xMIR162 59122xMON810xMIR604 59122xMIR162xMIR604 MON810xMIR162xMIR604 1507xMIR162 1507xMIR604 59122xMIR162 59122xMIR604 MON810xMIR162 MON810xMIR604 MIR162xMIR604 Category 3 Total Category 4** Total PIP Non-PIP Border Total NON-PIP TotalAR 2 1 14<1 <1 <1 2 <1 <1 <1 <1 <1 <13<1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <11191 45 14GA 1 1 13<1 <1 <1 2 <1 <1 <1 <1 <1 <13<1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <11181 34 12HI 1 1 13<1 <1 <1 2 <1 <1 <1 <1 <1 <13<1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <11181 34 12IA 2 1 14<1 <1 <1 2 <1 <1 <1 <1 <1 <13<1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <11191 45 14IL 1 1 13<1 <1 <1 2 <1 <1 <1 <1 <1 <13<1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <11181 34 12IN 1 1 13<1 <1 <1 2 <1 <1 <1 <1 <1 <13<1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <11181 34 12KS 1 1 13<1 <1 <1 2 <1 <1 <1 <1 <1 <13<1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <11181 34 12MN 1 1 13<1 <1 <1 2 <1 <1 <1 <1 <1 <13<1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <11181 34 12PR 1 1 13<1 <1 <1 2 <1 <1 <1 <1 <1 <13<1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <11181 34 12TN 1 1 13<1 <1 <1 2 <1 <1 <1 <1 <1 <13<1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <11181 34 12TX1113<1 <1 <1 2 <1 <1 <1 <1 <1 <13<1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <11181 34 12total353311 11 9046 136** Category 4 is non-experimental material that will be used for check plots only (not breeding).* The allocation of these acres among various event combinations represents best estimates as of this date and should not be used for inspection purposes. Environmental conditions (e.g.weather events, pest infestation levels), unforeseen regulatory datademands received from other countries, and other factors, may necessitate a reallocation of acres between protocols and traits. For event combinations where less than an acre may be grown, “<1” has been entered and the cumlative total of the "<1" acresis counted as one acre for the purpose of estimating acres within a Category across event combinations and protocol (as per BPPD guidance).Category 3Category 5Category 1Category 2Table 11. Estimated Acreage per State/Territory for Efficacy / IRM with No Isolation Protocol*

State 1507x59122xMON810 1507xMON810 59122xMON810 Category 1 Total 1507 59122 MON810 1507x59122 MIR604 Bt11 Bt11xMIR604 MIR162 Bt11xMIR162 Bt11xMIR162xMIR604 Category 2 Total Category 3 Category 4** Total PIP Non-PIP Border Total NON-PIP TotalAR41161 <1 <1 3 <1 <1 <1 <1 <1 <15 0 0 112 1315 26CO2114<1 <1 <1 2 <1 <1 <1 <1 <1 <130071 78 15DE31151 <1 <1 3 <1 <1 <1 <1 <1 <15 0 0 102 1315 25GA2114<1 <1 <1 2 <1 <1 <1 <1 <1 <130071 78 15IA821112 <1 <1 6 <1 <1 <1 <1 <1 <19 0 0 205 3035 55IL41161 <1 <1 3 <1 <1 <1 <1 <1 <15 0 0 113 1720 31IN31151 <1 <1 3 <1 <1 <1 <1 <1 <15 0 0 102 1315 25KS31151 <1 <1 3 <1 <1 <1 <1 <1 <15 0 0 102 1315 25KY2114<1 <1 <1 2 <1 <1 <1 <1 <1 <130071 45 12LA2114<1 <1 <1 2 <1 <1 <1 <1 <1 <130071 67 14MI2114<1 <1 <1 2 <1 <1 <1 <1 <1 <130071 45 12MN41161 <1 <1 4 <1 <1 <1 <1 <1 <16 0 0 122 1618 30MO31151 <1 <1 3 <1 <1 <1 <1 <1 <15 0 0 102 1315 25MS2114<1 <1 <1 2 <1 <1 <1 <1 <1 <130071 45 12NC2114<1 <1 <1 2 <1 <1 <1 <1 <1 <130071 67 14NE41161 <1 <1 4 <1 <1 <1 <1 <1 <16 0 0 123 182133NY2114<1 <1 <1 2 <1 <1 <1 <1 <1 <130071 78 15OH31151 <1 <1 3 <1 <1 <1 <1 <1 <15 0 0 102 1315 25OK2114<1 <1 <1 2 <1 <1 <1 <1 <1 <130071 45 12PA31151 <1 <1 3 <1 <1 <1 <1 <1 <15 0 0 102 1315 25SD31151 <1 <1 3 <1 <1 <1 <1 <1 <15 0 0 102 1315 25TN31151 <1 <1 3 <1 <1 <1 <1 <1 <15 0 0 102 1315 25TX2114<1 <1 <1 2 <1 <1 <1 <1 <1 <130072 1113 20WI2114<1 <1 <1 2 <1 <1 <1 <1 <1 <130071 56 13total119104 0 0 223306 529** Category 4 is non-experimental material that will be used for check plots only (not breeding).Category 5* The allocation of these acres among various event combinations represents best estimates as of this date and should not be used for inspection purposes.Environmental conditions (e.g.weather events, pest infestation levels), unforeseen regulatory data demands received from other countries, and other factors, maynecessitate a reallocation of acres between protocols and traits. For event combinations where less than an acre may be grown, “<1” has been entered and thecumlative total of the "<1" acres is counted as one acre for the purpose of estimating acres within a Category across event combinations and protocol (as per BPPDguidance).Category 1Category 2Table 12. Estimated Acreage per State/Territory for Seed Production Protocol*

State 1507x59122xMON810 1507xMON810 59122xMON810 Category 1 Total 1507 59122 MON810 1507x59122 MIR604 Bt11 Bt11xMIR604 MIR162 Bt11xMIR162 Bt11xMIR162xMIR604 Category 2 Total Category 3 Category 4** Total PIP Non-PIP Border Total Non-PIP TotalHI40 40 325405100 60 60 50 5 5 5 5 5 5300 0 0 705100 0100 805IL10 10 300320100 60 60 50 5 5 5 5 5 5300 0 0 620100 0100 720WA20 20 320360100 60 60 50 5 5 5 5 5 5300 0 0 660100 0100 760total 1085900 0 0 1985 300 2285** Category 4 is non-experimental material that will be used for check plots only (not breeding).Category 5* The allocation of these acres among various event combinations represents best estimates as of this date and should not be used for inspection purposes.Environmental conditions (e.g.weather events, pest infestation levels) unforeseen regulatory data demands received from other countries, and other factors, maynecessitate a reallocation of acres between protocols and traits.Category 2Category 1Table 13. Estimated Acreage per State/Territory for Regulatory Protocol*

State 1507x59122xMON810 1507xMON810 59122xMON810 Category 1 Total 1507 59122 MON810 1507x59122 MIR604 Bt11 Bt11xMIR604 MIR162 Bt11xMIR162 Bt11xMIR162xMIR604 Category 2 Total 1507x59122xMON810xMIR162 1507x59122xMON810xMIR604 1507x59122xMIR162xMIR604 1507xMON810xMIR162xMIR604 59122xMON810xMIR162xMIR604 1507x59122xMIR162 1507x59122xMIR604 1507xMON810xMIR162 1507xMON810xMIR604 1507xMIR162xMIR604 59122xMON810xMIR162 59122xMON810xMIR604 59122xMIR162xMIR604 MON810xMIR162xMIR604 1507xMIR162 1507xMIR604 59122xMIR162 59122xMIR604 MON810xMIR162 MON810xMIR604 MIR162xMIR604 Category 3 Total Category 4** Total PIP Non-PIP Border Total NON-PIP TotalAR 2 2 15<1 <1 <1 1 <1 <1 <1 <1 <1 <12<1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <11081 23 11IA 2 2 15<1 <1 <1 1 <1 <1 <1 <1 <1 <12<1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <11081 23 11IL 2 2 15<1 <1 <1 1 <1 <1 <1 <1 <1 <12<1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <11081 23 11IN 2 2 15<1 <1 <1 1 <1 <1 <1 <1 <1 <12<1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <11081 23 11KS 2 2 15<1 <1 <1 1 <1 <1 <1 <1 <1 <12<1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <11081 23 11LA 2 2 15<1 <1 <1 1 <1 <1 <1 <1 <1 <12<1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <11081 23 11MN 2 2 15<1 <1 <1 1 <1 <1 <1 <1 <1 <12<1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <11081 23 11MO 2 2 15<1 <1 <1 1 <1 <1 <1 <1 <1 <12<1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <11081 23 11NC 2 2 15<1 <1 <1 1 <1 <1 <1 <1 <1 <12<1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <11081 23 11NE 2 2 15<1 <1 <1 1 <1 <1 <1 <1 <1 <12<1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <11081 23 11OK 2 2 15<1 <1 <1 1 <1 <1 <1 <1 <1 <12<1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <11081 23 11SD 2 2 15<1 <1 <1 1 <1 <1 <1 <1 <1 <12<1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <11081 23 11TX 2 2 15<1 <1 <1 1 <1 <1 <1 <1 <1 <12<1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <11081 23 11WI2215<1 <1 <1 1 <1 <1 <1 <1 <1 <12<1 <1<1<1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <11081 23 11total702814 0 11242 154** Category 4 is non-experimental material that will be used for check plots only (not breeding).* The allocation of these acres among various event combinations represents best estimates as of this date and should not be used for inspection purposes. Environmental conditions (e.g.weather events, pest infestation levels), unforeseen regulatory datademands received from other countries, and other factors, may necessitate a reallocation of acres between protocols and traits. For event combinations where less than an acre may be grown, “<1” has been entered and the cumlative total of the "<1" acres iscounted as one acre for the purpose of estimating acres within a Category across event combinations and protocol (as per BPPD guidance).Category 5Category 1Category 2Category 3Table 14. Summary of EUP Program Quantities for 2009

State Category 1 Category 2 Category 3 Cry1F
(gm) Cry34/35Ab1
(gm) Cry1Ab
(MON810)

(gm) VIP3 (gm) Cry3a
(gm) Cry1Ab
(Bt11)
(gm)

AR 168.4 109.4 33.7 0.80 10.16 0.08 4.44 0.14 0.22

CA 126.3 117.8 16.8 0.66 8.47 0.07 4.18 0.13 0.24

CO 84.2 67.3 16.8 0.43 5.47 0.04 2.61 0.08 0.13

DE 42.1 42.1 0.0 0.21 2.74 0.02 1.30 0.04 0.08

GA 193.6 117.8 33.7 0.89 11.28 0.09 4.70 0.15 0.24

HI 3686.9 2735.7 16.8 16.56 210.68 1.70 85.33 2.75 5.47

IA 429.3 370.4 33.7 2.13 27.12 0.22 12.53 0.40 0.74

IL 2946.1 2735.7 33.7 14.60 186.02 1.51 85.85 2.77 5.47

IN 227.3 202.0 33.7 1.18 15.03 0.12 7.31 0.24 0.40

KS 151.5 117.8 33.7 0.77 9.85 0.08 4.70 0.15 0.24

KY 33.7 25.3 0.0 0.15 1.93 0.02 0.78 0.03 0.05

LA 75.8 42.1 8.4 0.33 4.14 0.03 1.57 0.05 0.08

MI 75.8 58.9 16.8 0.39 4.92 0.04 2.35 0.08 0.12

MN 168.4 143.1 33.7 0.88 11.20 0.09 5.48 0.18 0.29

MO 134.7 109.4 25.3 0.69 8.75 0.07 4.18 0.13 0.22

MS 67.3 50.5 16.8 0.34 4.38 0.04 2.09 0.07 0.10

NC 75.8 42.1 8.4 0.33 4.14 0.03 1.57 0.05 0.08

NE 218.9 193.6 25.3 1.12 14.23 0.12 6.78 0.22 0.39

NY 33.7 25.3 0.0 0.15 1.93 0.02 0.78 0.03 0.05

OH 42.1 42.1 0.0 0.21 2.74 0.02 1.30 0.04 0.08

OK 75.8 42.1 8.4 0.33 4.14 0.03 1.57 0.05 0.08

PA 84.2 84.2 16.8 0.47 5.99 0.05 3.13 0.10 0.17

PR 244.1 210.4 16.8 1.20 15.35 0.12 7.05 0.23 0.42

SD 126.3 101.0 25.3 0.64 8.21 0.07 3.91 0.13 0.20

TN 109.4 109.4 25.3 0.62 7.90 0.06 4.18 0.13 0.22

TX 159.9 126.3 33.7 0.82 10.40 0.08 4.96 0.16 0.25

WA 3030.3 2525.3 0.0 14.24 181.31 1.47 78.28 2.53 5.05

WI 134.7 101.0 25.3 0.67 8.49 0.07 3.91 0.13 0.20

total 12946 10648 539 62 787 6 347 11 21

Assumptions:

3) The g/kg of seed used for 1507 (0.0024 g/kg Cry1F), 59122 (0.031g/kg Cry34Ab1) and MON810 (0.00027 g/kg Cry1Ab) were based on the values observed in PHI-2008-006 (MRID 475109-04). The amount of a.i for MIR162 (0.031 g/kg VIP3A), MIR604 (0.001 g/kg mCry3A) and Bt11 (0.002 g/kg Cry1Ab) were based on the levels presented in Syngenta EUP application for MIR162. kgs of seed per Category

1) Assumed 100% of the EUP test plants (Category 1) were 1507x59122xMON810 (0.0027g/kg Cry1F, 0.034 g/kg Cry34/35Ab1 and 0.00026g/kg Cry1Ab). Values based on expression levels observed in PHI-2008-006 (MRID 475109-04).

2) Assumed 100% of the acres in Category 2 and Category 3 were planted to each registered single event (i.e. 1507, 59122, MON810, MIR162 and MIR604) except for Bt11 (Category 2 only). Total A.I. QuantitiesG3. Details of the Proposed EUP Program

Crop: Corn (Zea mays L.)

Target Pests: Agrotis ipsilon (Black cutworm) Helicoverpa zea (Corn earworm or cotton bollworm)

Ostrinia nubilalis (European corn borer) Spodoptera frugiperda (Fall armyworm) Diatraea grandiosella (Southwestern corn borer)

Richia albicosta (Western bean cutworm) Elasmopalpus lignosellus (Lesser corn stalk borer)

Diatrae crambidoides (Southern corn stalk borer)

Diatrea saccharalis (Sugarcane borer) Papaipema nebris (Common stalk borer) Diabrotica virgifera virgifera (western corn rootworm)

Diabrotica barberi (northern corn rootworm) Diagrotica virgifera zeae (Mexican corn rootworm)

Major Geographic Areas: 27 states and Puerto Rico, representing diverse agronomic zones

Sites: Pioneer research centers, seed production fiel ds, GLP cooperator fields, privately owned farms and university farms and testing sites. EUP Bloc ks may or may not be located at EPA registered pesticide-producing establishments. All EUP Bl ocks will be planted on existing agricultural land.

Period of Use: April 1, 2009 through March 31, 2010.

Protocols for Field Experimentation: Research planned under the EUP Program will be conducted under the following six protocols: 1. Nursery / Breeding; 2. Yield / Agronomic; 3. Efficacy / IRM; 4. Efficacy / IRM with No Isolation; 5. Seed Production; and 6. Regulatory.

G4. Definitions

EUP Test Plants – Plants that contain 1507x59122xMON810, 1507xMON810 or

59122xMON810.

Experiment -- An Experiment is the unit of measure used to coordinate and track specific field activities. Experiment ID’s are assigned to each Experiment.

EUP Experiment - Experiments containing EUP Test Pl ants are defined as EUP Experiments. EUP Experiments may also contain registered PI Ps for breeding or comparison (if listed on the Master Label), non-registered PIPs (if listed on t he Master Label), registered PIPs for comparison only ( i.e ., “commercial checks”), and/or non-PIPs (suc h as herbicide-tolerant or non-transgenic corn).EUP Block - The EUP Block is defined as an area of land in which EUP Experiments are planted (including filler corn, if planted between EUP Experimen ts). An EUP Block may consist of one, several or many EUP Experiments. In cases where the EU P Block is planted directly adjacent to non-EUP plants, the EUP Block will be separ ated from non-EUP plants by an a lleyway (approximately 10 feet wide) or by a minimum 4 rows (approximately 10 feet wide) of purple-colored corn. The alleyway or purple-colored corn serves as a means of separa ting the EUP Block from the non-EUP corn. See Figures 1 and 2 for examples of EU P Block configurations. The alleyway or purple-colored corn is not included in the acreage of the EUP Block.

G5. Universal Protocol Procedures The following procedures will be used for all EUP Experiments.

Shipping and Labeling

 Each shipment containing EUP seed must contai n a copy of the EUP Master Label. A Master Label Weight Summary may be used to summarize the amount of seed in the shipment, so long as it accompanies the EUP Master Label. See Figure 3 for an example of a Master Label Weight Summary.

 Seed will be shipped within two layers of packagi ng material consisting of a primary container within a secondary container, each of which must be independently capable of preventing the loss

of seed.

 Packaging from seed shipments is typically destroyed after shipments have been received.

 Documentation will be kept of seed received, planted, harvested, shipped and destroyed.

Schedule

 For the continental U.S., planting of trials gen erally takes place from early March through the month of June. Observations and harvesting generally occur after planting through August to November. For Hawaii and Puerto Rico, there may be multiple plantings that occur throughout the

year.

Planting

 Seed may be planted by hand or machine.

o Do not re-use seed packets.

o Dispose of any remaining seed according to accepted disposition methods (see “Final Disposition of EUP Material” in each protocol).

 In cases where the EUP Block is planted direct ly adjacent to non-EUP plants, the EUP Block will be separated from non-EUP plants by an alleyway (approximately 10 ft wide) or by a minimum 4 rows (approximately 10 feet wide) of purple-col ored corn. The alleyway or purple-colored corn serves as a means of separating the EUP Block from the non-EUP corn.

 After planting, planters will be cl eaned while in the EUP Block.

 Border rows may be used for agronomic purposes, but are not required under this EUP Program.

Post-Trial Monitoring

 EUP Blocks will be managed for volunteer plants to prevent persistence of EUP Test Plants in the environment. Any volunteer plants found will be destroyed using an appropriate herbicide or by mechanical means before reaching reproductive stage.

 Upon full federal and state EPA approval of the EUP Test Plants, all post-trial monitoring will cease immediately.Storing EUP Material

 EUP Test Plant seed will be stored in a secure facility with a sign stating that regulated, genetically modified material is stored inside.

 EUP Test Plant seed will be labeled to enable ready identification of materials. Examples of labeling include (but are not limited to) color coding, marking, tagging, or otherwise distinguishing seed to allow immediate recognition.

G6. Special Protocol Exemptions

Efficacy / IRM with No Isolation

Pioneer is requesting that the Efficacy / IRM Prot ocol with No Isolation not be required to have 660 feet. isolation from other corn, including commercia l corn. The reasons for this are several-fold: 1. This protocol contains only EUP Test Pl ants, Registered PIPs, and Non-PIP corn. Each individual event is registered, and all PIP proteins have full tolerance exemptions. 2. Under this protocol, there will be no non-regi stered PIP plants (Category 3) (see Table 12). 3. The single events have been fully evaluated for potential environmental effects. Data

demonstrate that 1507 and MON810 are additive (not synergistic or antagonistic) against several key pests (European corn borer, sout hwestern corn borer, corn earworm and fall armyworm). Monarch larvae studies demonstrat e that there are no adverse effects of the EUP Test Plants. Expression levels (as measured by quantitative ELISA) in various tissues of

the EUP Test Plants are similar to the individual events that comprise them. Therefore, we do not anticipate any adverse environmental impact. 4. We will be conducting experime nts with key academic stakehol ders. Because many university

farms/locations do not have isolated locati ons, requiring isolation would prohibit most academics from participating in the Efficacy / IRM program. 5. We will be screening for efficacy against sec ondary pests that can’t be reared in culture ( e.g. western bean cutworm). The best data come from corn fields with historical insect pressure,

and often these locations do not have 66 0 ft. isolation from commercial corn. 6. All grain and vegetative material produced under the Efficacy / IRM Protocol with No Isolation will be destroyed. Grain will not enter the food/feed stream. 7. The proposed acreage under this protocol is relatively low (less than 84 acres of EUP Test Plants). In addition, the proposed EUP program is for a relatively short duration (one growing season). Therefore, environmental exposure to the EUP Test Plants will be minimal.

Seed Production

Pioneer is requesting the option of using unwanted seed and vegetati ve material generated under the Seed Production Protocol for local animal feed and bedding. The reasons for this are several-fold: 1. This protocol contains only EUP Test Pl ants, Registered PIPs, and Non-PIP corn. Each individual event is registered, and all PIP proteins have full tolerance exemptions. 2. Under this protocol, there will be no non-regi stered PIP plants (Category 3) (see Table 13). 3. This option is consistent with typical dispos al practices at some Pioneer inbred production

facilities. 4. Expression levels (as measur ed by quantitative ELISA) in va rious tissues of the EUP Test

Plants are similar to the individual events that comprise them. Therefore, we do not anticipate increased exposure. 5. The proposed EUP program is for a re latively short duration (12 months).Figure 3. Example of Master Label Weight Summary



## Master Label Weight Summary

29964-EUPEvent Weight (lbs)

1507xMON810 0.1

1507x59122xMON810 0.1

1507 0.05

MON810 0.05

Bt11xMIR162 0.03

Bt11 0.03

MIR162 .02

59122 .02

MIR604 .01Nursery / Breeding Protocol

General Objectives

 Introgress events 1507, 59122 and MON810 into elite inbred corn lines.

 Produce research hybrid seed for evaluation in field, greenhouse and/or laboratory trials.

 Collect phenotypic data on converted inbred lines.

 Collect plant tissue on converted inbred lines for laboratory analysis.

Description

Inbreds may be hand pollinated (selfed, backcrossed or crossed to tester lines) to produce new inbred and hybrids for evaluation. Some inbreds may be op en pollinated in isolation to produce seed for phenotypic analysis. Plants may be treated with herb icides and/or insecticides. Seed is generally

retained for future breedi ng or testing purposes.

Gene Containment

One or more of the following practices will be used:

1. Spatial isolation

Option A. The EUP Block will be separated from any sexually compatible corn by at least 660

feet.

Option B. Corn can be grown within 660 feet of an EUP Block. All open-pollinated grain within 660 feet of the EUP Block will be disposed as stated in “Final Disposition of the EUP Material.”

Option C. Hand pollinations of non-EUP plants can occur within 660 feet of an EUP Block. Seed resulting from such hand-pollinations may be retained for future breeding and product development purposes if: 1) The hand-pollinated corn plants are pollinated with pollen from non-EUP plants; and 2) The ear shoots of the hand-pollinated corn plants are covered for the period of time during which the silks of the plants are sexually receptive. Remaining open-pollinated grain within 660 feet of the EUP Block will be disposed as stated in “Final Disposition of the EUP Material.”

2. Temporal isolation

The flowering period for plants within the EUP Blo ck will not coincide with the presence of sexually receptive corn within 660 feet.

3. Reproductive isolation

The EUP Test Plants and non-registered PIP plant s will be detasseled or destroyed prior to the

onset of anthesis.Harvest Procedures

 Hand or machine harvest.

 Clean combine or picker in the regulated transgenic site.

 Store retained seed in a clearly labeled and secure facility.

 After harvest, cultivate remaining vegetative material into the regulated transgenic site.

Final Disposition of EUP Material

Final disposition of EUP seed or grain not sav ed for further breeding or testing purposes will be accomplished using one of the methods below.

o cultivation into soil at the regulated transgenic site;

o incineration;

o burial (at least two feet deep);

o composting;

o autoclaving;

o disposal at an approved landfill.

Upon full federal and state EPA approval of the EUP Test Plants, all disposition restrictions will cease immediately.Yield / Agronomic Protocol

General Objectives

 Assess the agronomic impact of combini ng the 1507, 59122 and MON810 events in various hybrid backgrounds under various environm ental and insect pressure conditions.

 Assess the yield and agronomic properties of EUP test inbreds and hybrids compared to other single and stacked event inbreds and hybrids (both registered and non- registered PIP single events and breeding stacks), commercial che ck hybrids (registered PIPs), conventional hybrids, and/or insecticide treatments.

 Assess the herbicide tolerance of EUP Test Plants (including EUP Test Plants that have been crossed with non-PIP herbicide tolerant lines of corn).

Description

EUP Blocks will generally be planted in multiple locations using ra ndomized block desi gn, strips or single large blocks depending on study type. Seed ma y be treated with a variety of insecticidal seed treatments or blended with non-transgenic or tran sgenic corn to evaluate agronomic performance including yield. Plants may be treated with herbicide and/or insecticides. Commercial checks may be planted as comparators. Harvested grain is generally not retained for breeding purposes.

Gene Containment

One or more of the following practices will be used:

1. Spatial isolation

Option A. The EUP Block will be separated from any sexually compatible corn by at least 660

feet.

Option B. Corn can be grown within 660 feet of an EUP Block. All open-pollinated grain within 660 feet of the EUP Block will be disposed as stated in “Final Disposition of the EUP Material.”

Option C. Hand pollinations of non-EUP plants can occur within 660 feet of an EUP Block. Seed resulting from such hand-pollinations may be retained for future breeding and product development purposes if: 1) The hand-pollinated corn plants are pollinated with pollen from non-EUP plants; and 2) The ear shoots of the hand-pollinated corn plants are covered for the period of time during which the silks of the plants are sexually receptive. Remaining open-pollinated grain within 660 feet of the EUP Block will be disposed as stated in “Final Disposition of the EUP Material.”

2. Temporal isolation

The flowering period for plants within the EUP Blo ck will not coincide with the presence of sexually receptive corn within 660 feet.

3. Reproductive isolation

The EUP Test Plants and non-registered PIP plant s will be detasseled or destroyed prior to the

onset of anthesis.Harvest Procedures

 Hand or machine harvest.

 Clean combine or picker in the regulated transgenic site.

 Store retained seed in a clearly labeled and secure facility.

 After harvest, cultivate remaining vegetative material into the regulated transgenic site.

Final Disposition of EUP Material

Final disposition of EUP seed or grain not sav ed for further breeding or testing purposes will be accomplished using one of the methods below.

o cultivation into soil at the regulated transgenic site;

o incineration;

o burial (at least two feet deep);

o composting;

o autoclaving;

o disposal at an approved landfill.

Upon full federal and state EPA approval of the EUP Test Plants, all disposition restrictions will cease immediately.Efficacy / IRM Protocol

General Objectives

 Assess the efficacy of the EUP Test Plants against key target and secondary pests in corn.

 Generate data for use in the refinement of IR M strategies for corn containing events 1507,

MON810 and/or 59122.

 Characterize the refuge deployment methods for the EUP Test Plants.

Description

Test plots will generally be planted in multiple locati ons using randomized block design, strips or single large blocks depending on study type. Seed may be treated with a variety of insecticidal seed treatments or blended with non-transgenic or transg enic corn to evaluate various methods of refuge deployment. Artificial or natural infestations of lepidopteran and coleopteran may be used in these trials. Plants may be treated with herbicide and/or insecticides. Harvested grain is generally not retained for breeding purposes.

Gene Containment

One or more of the following practices will be used:

1. Spatial isolation

Option A. The EUP Block will be separated from any sexually compatible corn by at least 660

feet.

Option B. Corn can be grown within 660 feet of an EUP Block. All open-pollinated grain within 660 feet of the EUP Block will be disposed as stated in “Final Disposition of the EUP Material.”

Option C. Hand pollinations of non-EUP plants can occur within 660 feet of an EUP Block. Seed resulting from such hand-pollinations may be retained for future breeding and product development purposes if: 1) The hand-pollinated corn plants are pollinated with pollen from non-EUP plants; and 2) The ear shoots of the hand-pollinated corn plants are covered for the period of time during which the silks of the plants are sexually receptive. Remaining open-pollinated grain within 660 feet of the EUP Block will be disposed as stated in “Final Disposition of the EUP Material.”

2. Temporal isolation

The flowering period for plants within the EUP Blo ck will not coincide with the presence of sexually receptive corn within 660 feet.

3. Reproductive isolation

The EUP Test Plants and non-registered PIP plant s will be detasseled or destroyed prior to the

onset of anthesis.Harvest Procedures

 Hand or machine harvest.

 Clean combine or picker in the regulated transgenic site.

 Store retained seed in a clearly labeled and secure facility.

 After harvest, cultivate remaining vegetative material into the regulated transgenic site.

Final Disposition of EUP Material

Final disposition of EUP seed or grain not sav ed for further breeding or testing purposes will be accomplished using one of the methods below.

o cultivation into soil at the regulated transgenic site;

o incineration;

o burial (at least two feet deep);

o composting;

o autoclaving;

o disposal at an approved landfill.

Upon full federal and state EPA approval of the EUP Test Plants, all disposition restrictions will cease immediately.Efficacy / IRM Protocol with No Isolation

General Objectives

 Assess the efficacy of the EUP Test Plants against key target and secondary pests in corn.

 Generate data for use in the refinement of IR M strategies for corn containing events 1507,

MON810 and/or 59122.

 Characterize the refuge deployment methods for the EUP Test Plants.

Description

Test plots will generally be planted in multiple locati ons using randomized block design, strips or single large blocks depending on study type. Seed may be treated with a variety of seed treatments and/or blended with non-transgenic or transgenic corn. Arti ficial or natural infestations of lepidopteran and coleopteran may be used in these trials. Plants ma y be treated with herbicide and/or insecticides. Harvested grain is generally not retained for breeding purposes.

Gene Containment

No containment or isolation procedures are requir ed. Other corn, including commercial corn, may be grown within 660 feet of the EUP Block (see Figure 2). Purple-colored corn or an alleyway will separate the EUP Block from adjacent corn (see “Planting” language in Section G5).

Harvest Procedures

 Hand or machine harvest.

 Clean combine or picker in the regulated transgenic site.

 Store retained seed in a clearly labeled and secure facility.

 After harvest, cultivate remaining vegetative material into the regulated transgenic site.

Final Disposition of EUP Material

Final disposition of EUP seed or grain not sav ed for further breeding or testing purposes will be accomplished using one of the methods below.

o cultivation into soil at the regulated transgenic site;

o incineration;

o burial (at least two feet deep);

o composting;

o autoclaving;

o disposal at an approved landfill.

Upon full federal and state EPA approval of the EUP Test Plants, all disposition restrictions will cease immediately.Seed Production Protocol

General Objectives

 Increase inbred and hybrid seed for further breeding and testing.

Description

EUP Blocks (approximately 0.1 to 15 acres in size ) of individual inbred lines may be increased by open pollination. Hybrid seed may be produced (in EUP Blocks approximately 1 to 30 acres in size) by planting two inbred lines per hybrid produced and allowing open pollination (by using a male sterile inbred or removing the tassels from one of the inbreds). Plants may be treated with

herbicides and/or insecticides.

Gene Containment

One or more of the following practices will be used:

1. Spatial isolation

Option A. The EUP Block will be separated from any sexually compatible corn by at least

660 feet.

Option B. Corn can be grown within 660 feet of an EUP Block. All open-pollinated grain within 660 feet of the EUP Block will be dispos ed as stated in “Final Disposition of the EUP

Material.”

Option C. Hand pollinations of non-EUP plants c an occur within 660 feet of an EUP Block. Seed resulting from such hand-pollinations may be retained for future breeding and product development purposes if: 1) The hand-pollinated corn plants are pollinated with pollen from non-EUP plants; and 2) The ear shoots of the hand-pollinated corn plants are covered for the period of time during which the silks of the pl ants are sexually receptive. Remaining open-pollinated grain within 660 feet of the EUP Block will be disposed as stated in “Final Disposition of the EUP Material.”

2. Temporal isolation

The flowering period for plants within the EUP Block will not coincide with the presence of sexually receptive corn within 660 feet.

3. Reproductive isolation

The EUP Test Plants and non-registered PIP pl ants will be detasseled or destroyed prior to

the onset of anthesis.

Harvest Procedures

 Hand or machine harvest.

 Clean combine or picker in the regulated transgenic site.

 Store retained seed in a clearly labeled and secure facility.

 After harvest, cultivate remaining vegetative material into the regulated transgenic site.Final Disposition of EUP Material

Unwanted seed, grain and plant material (such as cobs and husks) may be used for animal feed and/or bedding. Such material may also be disposed of using one of the methods below:

o cultivation into soil at the regulated transgenic site;

o incineration;

o burial (at least two feet deep);

o composting;

o autoclaving;

o disposal at an approved landfill.

Upon full federal and state EPA approval of the EUP Test Plants, all disposition restrictions will cease immediately.Regulatory Protocol

General Objectives:

 Conduct field trials as needed to support global regulatory submissions

 Generate plant tissue samples from EUP Test Plants (inbreds and hybrids) for use in regulatory characterization studies.

 Collect phenotypic data for use in global regulatory submissions

 Produce grain for use in animal feeding studies

Description

Test plots will generally be planted in multiple lo cations using randomized block design, strips or single large blocks depending on study type. Agr onomic characteristics and plant tissue samples may be collected at various developmental growth stages. Plants may be treated with herbicide

and/or insecticides.

Gene Containment

One or more of the following practices will be used:

1. Spatial isolation

Option A. The EUP Block will be separated from any sexually compatible corn by at least

660 feet.

Option B. Corn can be grown within 660 feet of an EUP Block. All open-pollinated grain within 660 feet of the EUP Block will be dispos ed as stated in “Final Disposition of the EUP

Material.”

Option C. Hand pollinations of non-EUP plants c an occur within 660 feet of an EUP Block. Seed resulting from such hand-pollinations may be retained for future breeding and product development purposes if: 1) The hand-pollinated corn plants are pollinated with pollen from non-EUP plants; and 2) The ear shoots of the hand-pollinated corn plants are covered for the period of time during which the silks of the pl ants are sexually receptive. Remaining open-pollinated grain within 660 feet of the EUP Block will be disposed as stated in “Final Disposition of the EUP Material.”

2. Temporal isolation

The flowering period for plants within the EUP Block will not coincide with the presence of sexually receptive corn within 660 feet.

3. Reproductive isolation

The EUP Test Plants and non-registered PIP pl ants will be detasseled or destroyed prior to

the onset of anthesis.Harvest Procedures

 Hand or machine harvest.

 Clean harvest equipment ( i.e ., combine) while in the regulated transgenic site.

 Store retained seed in a clearly labeled and secure facility.

 After harvest, cultivate remaining vegetative material into the regulated transgenic site.

Final Disposition of EUP Material

Final disposition of EUP seed or grain not sav ed for further breeding or testing purposes will be accomplished using one of the methods below.

o cultivation into soil at the regulated transgenic site;

o incineration;

o burial (at least two feet deep);

o composting;

o autoclaving;

o disposal at an approved landfill.

Upon full federal and state EPA approval of the EUP Test Plants, all disposition restrictions will cease immediately.CONFIDENTIAL ATTACHMENT

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