OCS Study 

MMS 2006-071 

 

Coastal Marine Institute 

Annotated Bibliography 

of the Potential Environmental Impacts 

of Chlorination and Disinfection 

Byproducts Relevant to Offshore 

Liquefied Natural Gas Port Facilities 

Cooperative Agreement

Coastal Marine Institute 

Louisiana State University 

U.S. Department of the Interior

Minerals Management Service 

Gulf of Mexico OCS Region 
U.S. Department of the Interior Cooperative Agreement 

Minerals Management Service Coastal Marine Institute 

Gulf of Mexico OCS Region Louisiana State University 

OCS Study 

 MMS 2006-071 

Coastal Marine Institute 

Annotated Bibliography 

of the Potential Environmental Impacts 

of Chlorination and Disinfection 

Byproducts Relevant to Offshore 

Liquefied Natural Gas Port Facilities 

Authors 

Richard F. Shaw 

Kevin W. Baggett 

December 2006 

Prepared under MMS Contract 

1435-01-04-CA-32806-39264 

by 

Louisiana State University 

Coastal Fisheries Institute 

Department of Oceanography and Coastal Sciences 

School of the Coast and Environment 

Baton Rouge, Louisiana 70803 

Published by 
 DISCLAIMER 

This report was prepared under contract between the Minerals Management Service 

(MMS) and Louisiana State University. This report has been technically reviewed by the 

MMS and has been approved for publication. Approval does not signify that the contents 

necessarily reflect the view and policies of the MMS, nor does mention of trade names or 

commercial products constitute endorsement or recommendation for use. It is, however, 

exempt from review and in compliance with MMS editorial standards.

REPORT AVAILABILITY 

Extra copies of this report may be obtained from the Public Information Office at the 

following address: 

 U.S. Department of the Interior 

 Minerals Management Service 

 Gulf of Mexico OCS Region 

 Public Information Office (MS 5034) 

 1201 Elmwood Park Blvd. 

 New Orleans, LA 70123-2394 

Telephone: (504) 736-2519 or 

 1-800-200-GULF 

CITATION 

Suggested Citation: 

Shaw, R.F. and K.W. Baggett. 2006. Annotated bibliography of the potential 

environmental impacts of chlorination and disinfection byproducts relevant to 

offshore liquefied natural gas port facilities. U.S. Dept. of the Interior, Minerals 

Management Service, Gulf of Mexico OCS Region, New Orleans, LA. OCS Study 

MMS 2006-071. 112 pp. 

ABOUT THE COVER 

Cover photo courtesy of Chevron Texaco web page 

http://www.chevron.com/about/our_businesses/docs/2003_Port_Pelican_brochure.pdf

© 2006 Chevron Corporation. 

Used with permission. All rights reserved. 

iii 
ACKOWLEDGMENTS 

This compilation of citations was prepared with the assistance of several electronic 

databases subscribed to by Louisiana State University. The databases were searched 

electronically using the Cambridge Scientific Abstracts Internet Database Service (CSA), 

the U.S. Government Printing Office Access service (GPO), the Scholarly J. Archive 

(JSTOR), the Institute for Scientific Information (ISI), and Ovid Technologies database 

services. The following databases were searched: 

Academic Search Premier 

Aquaculture Abstracts (ASFA) 

Aquatic Pollution and Environmental Quality (ASFA) 

Biological Abstracts 

Biological Sciences (CSA) 

Biological Sciences and Living Resources (CSA) 

Catalog of US Government Publications 

Conference Papers Index 

Dissertation Abstracts 

Effects of Offshore Oil and Gas Development 

Electronic Collections Online (ECO) 

Energy Citations Database (Gov) 

GEOBASE 

Grey Literature 

JSTOR (Ecology) 

Marine Biotechnology Abstracts 

National Technical Information Service 

Oceanic Abstracts 

Science.gov 

Toxnet 

Zoological Record 

v 
TABLE OF CONTENTS 

Introduction..........................................................................................................................1 

Background and Motivation ................................................................................................3 

Methodology........................................................................................................................5 

List of Keywords Searched..................................................................................................7 

Annotated Bibliography.......................................................................................................9 

Subject Index .....................................................................................................................87 

Genus Species Index .........................................................................................................97 

vii 
INTRODUCTION 

This research specifically addresses a Minerals Management Service (MMS) “Special 

Task Area” and also responds to urgent informational needs of the Environmental 

Protection Agency, the U.S. Coast Guard, NOAA’s National Marine Fisheries Service 

(NOAA NMFS), and northern Gulf of Mexico state natural resource agencies. We 

conducted an extensive literature search and compiled an indexed, annotated 

bibliography on the toxicity of intake chlorinated waters and their disinfection byproducts 

associated with proposed offshore, open-loop (flow-through), Liquefied Natural 

Gasification (LNG) terminals and the potential environmental consequences that will 

result when these substances are discharged into the marine environment. 

Consideration of the application and licensing of these offshore LNG facilities with openloop, intake water circulation systems is on-going. Environmental concerns still remain 

within the regulatory oversight agencies, the commercial and recreational fishing 

industries, and with the public-at-large. These concerns have specifically focused on the 

anticipated accumulative high mortalities of commercially- and recreationally-important 

ichthyoplankton and zooplankton (i.e., decapods or crabs and shrimp). These mortalities 

will result from the impingement, entrainment, toxic exposure to chlorination and 

disinfection byproducts (DBPs) associated with treatment of intake waters, and from cold 

shock during the operation of these proposed LNG open-loop systems. 

1 
BACKGROUND AND MOTIVATION

On November 25, 2002, Congress amended the Deepwater Port Act of 1974 (DWPA) by 

passing Section 106 of the Maritime Transportation Security Act. Section 106 made the 

following licensing and regulatory responsibility changes: 1) It redefined, under the 

DWPA, the Secretary of Transportation’s authorization to issue licenses to own, 

construct, and operate deepwater ports (i.e., floating or man-made structure other than 

vessels in federal waters) for the purposes of storing, transporting, or handling oil, to also 

include natural gas; and 2) It transferred oversight of offshore natural gas terminals from 

the Federal Energy Regulatory Commission to the Maritime Administration (MARAD) 

with the Department of Transportation (DOT) and the U.S. Coast Guard (USCG now in 

the Department of Homeland Security having moved from DOT). To date, the USCG 

has received 14 applications for Deepwater Port Licenses for proposed Liquefied Natural 

Gas (LNG) Terminals in the northern Gulf of Mexico. As presently proposed, all but two 

of these offshore, “platform”/port facilities will need very large volumes of subsurface 

seawater (probably taken from a staggered array of intake ports located between 10-30m 

depth) to warm and re-gasify the LNG. These ambient waters which will be chlorinated 

and subsequently chilled will then have to be discharged back into the marine 

environment, probably through a turbulent diffuser system. Since these proposed LNG 

facilities will utilize such an “open-loop” warming system, the various regulatory 

agencies involved have expressed concerns about possible effects associated with this 

technology. Namely, that the accumulated impacts of impingement on intake screens and 

entrainment within the chlorinated and chilled processed waters within these systems 

may result in significant adverse effects on the early life history stages of fish and 

decapods and their subsequent adult populations especially for managed species along the 

AL-MS-LA-TX continental shelf. 

On February 16, 2005, MARAD’s most recent open-loop decision was handed down 

granting Shell U.S. Gas and Power’s license to operate the Gulf Landing LNG terminal in 

West Cameron Block 213, 38 statute miles off Cameron, LA. The terminal will be 

capable of processing 1 billion cubic feet of gas per day, necessitating that more than 136 

million gallons of sea water per day be pumped through its radiator-like system. Two 

other licenses have been granted: ChevronTexaco (Port Pelican, 36 miles off southwest 

LA-Vermilion Block 140, first to receive a license on March 31, 2004, for a 176 million 

gal/day facility); and Excelerate Energy (Gulf Gateway Energy Bridge Deepwater Port, a 

submersible buoy 116 miles off Cameron, LA, in 91 meters of water, which requires a 

ship that can regasify the LNG onboard under a license to operate at a peak volume of 76 

million gal/day of seawater for 248 days/year) which is now operational (March 20, 

2005) and has already offloaded three shipments of LNG. There are presently two (2) 

other LNG open-loop terminals proposed for the north-central Gulf, i.e., Conoco-Phillips 

(Beacon Port Clean Energy Terminal, High Island Block 71, 56 miles off the LA-TX 

border – presently under review); and Torp Technology’s Bienville LNG Offshore 

Energy Terminal, BOET (Main Pass Block 258, 63 miles south of Mobile Point, AL). 

Freeport McMoRan Main Pass Energy Hub (Main Pass Block 299, 37 miles east of 

Venice, LA) recently received (January 3, 2007) approval of its application, which it had 

amended proposing a closed rack system that will use very little ambient seawater. 

3 
Finally, both ExxonMobil (Pearl Crossing, 41 miles SE of Cameron) and Conoco-Phillips 

(Compass Point, 11 miles off Dauphin Island, AL) have withdrawn their open-loop 

applications. 

Therefore, to meet the information needs of MMS, EPA, USCG, NMFS/NOAA and state 

regulatory agencies, we have provided an annotated bibliography on reported research 

concerning the affects of chlorinated marine waters and their resultant DBPs in water 

intake and discharge systems, especially as they relate to marine and estuarine 

ichthyoplankton and zooplankton likely to be entrained within the intake waters of 

offshore LNG port facilities. 

4 
METHODOLOGY

We have conducted an on-line search of all potentially-relevant databases and then 

compiled an annotated bibliography, based upon the pertinent literature, i.e., peerreviewed and non-peer-reviewed literature as well as the “grey literature” (e.g., 

government documents, conference proceedings, major technical reports, etc.) relevant to 

coastal/marine pelagic communities within the northcentral Gulf of Mexico. We focused 

particularly on the effects of chlorinated waters and associated DBPs and their influence 

on larval/juvenile fishes and commercially- or recreationally-important invertebrates (i.e., 

decapods or shrimp and crabs). 

The criteria used to determine whether a reference might be useful or not had to be 

adjusted as the search progressed. Only references related to the effects of chlorination, 

resultant DBPs or chlorinated water discharge on the affected species or on the chemical 

composition of the receiving marine and estuarine environments were selected. No 

consideration was given for other closely related topics, such as fish impingement or 

entrainment associated with open-loop or flow-through water systems, which may have 

been covered elsewhere (e.g., Martinez-Andrade, F. and D.M. Baltz. 2003. Marine and 

coastal fishes subject to impingement by cooling-water intake systems in the northern 

Gulf of Mexico: An annotated bibliography. U.S. Dept. of the Interior, Minerals 

Management Service, Gulf of Mexico OCS Region, New Orleans, LA. OCS Study MMS 

2003-040. 113 pp.). 

The search strategy included preparation of initial keyword and database lists. All data 

bases available for searching in the library at Louisiana State University and relevant to 

aquatic Biology were queried to search article titles, abstracts, keyword lists, and any 

other available text in the databases using the list of key words. Eleven key words or 

phrases, root forms, and logical operators (e.g., AND & OR) were used to develop the 

bibliography (see below List of Keywords Searched). This search captured documents 

from 1964 to May 2006. 

Several thousand documents were found using this initial method of searching through 

the selected databases. During several subsequent screening and review processes, close 

to 200 documents were finally selected for inclusion into the finished work. Some data 

bases, especially those dealing with the grey literature or those citations before 1980, did 

not provide abstracts. 

Finally, each selected article was categorized and cross-referenced by subject matter. A 

subject index of these categories was created which points the user of the bibliography to 

the appropriate articles on the subjects by listing the authors and year of publication for 

each article under the subject heading. A genus species index, which points the user to 

articles that treat the taxa listed, was also created in this manner. 

5 
LIST OF KEYWORDS SEARCHED 

chlorination 

disinfection byproducts 

intake water 

discharge water 

cooling water 

heating water 

entrainment 

power plant 

ichthyoplankton 

zooplankton 

marine organisms 

7 
ANNOTATED BIBLIOGRAPHY 

Abarnou, A. and L. Miossec. 1992. Chlorinated waters discharged to the marine 

environment chemistry and environmental impact: An overview. Science Total 

Environment 126(1-2): 173-197. 

 Chlorinated discharges into coastal waters are of great environmental concern 

because of the large amount of chlorine and the diversity of the compounds involved. 

Seawater chlorination for cooling systems and disinfection of urban wastewaters are the 

major sources of these hazardous compounds reaching the marine environment. 

Seawater chlorination (at the mg/L level) produces bromine which disappears rapidly in 

receiving waters yielding organobrominated compounds. Chlorination of urban 

wastewaters, containing high concentrations of organic carbon and ammonia, requires 

higher chlorine dosage (5-20 mg/L) to fulfill the disinfection requirements. 

Monochloramine still active is the predominant final compound. Organochlorinated byproducts represent a minor part of the added compound; however they need further 

investigations to precisely evaluate their potential hazard to marine life because of their 

persistence and mutagenic character. 

Ahamed, M.S., K. Suresh, G. Durairaj and K.V.K. Nair. 1993. Effect of cooling water 

chlorination on primary productivity of entrained phytoplankton at Kalpakkam, east coast 

of India. Hydrobiologia 271(3): 165-168. 

 We studied the effect of various concentrations of chlorine, used in the cooling 

system of Madras Atomic Power Station (MAPS), on the primary productivity of 

entrained phytoplankton from October 1988 to December 1990. The rates of primary 

productivity (gross) were lower at the discharge point than in the intake area. The 

reduction in productivity ranged from 30 to 70% at low-dose chlorination (residual 

chlorine 0.05-0.20 mg/L at the discharge point). During shock-dose chlorination, when 

residual chlorine at the discharge point ranged from 1.10 to 1.50 mg/L, the reduction in 

productivity was 80-83%. A reduction of 16-17% was recorded without chlorination. 

Alderson, R. 1969. The survival of flatfish eggs, larvae, and post-larvae in low 

concentrations of free chlorine. Report No. ICES 27, International Council for the 

Exploration of the Sea, Port Erin, United Kingdom. 

 No abstract. 

Alderson, R. 1972. The effects of low concentrations of free chlorine on eggs and larvae 

of plaice, Pleuronectes platessa L. Pp. 312-315. In: M. Ruvio (ed). Marine Pollution 

and Sea Life. Fishing News Books Ltd., Surrey, England. 

 No abstract. 

9 
Alderson, R. 1974. Sea-water chlorination and the survival and growth of the early 

developmental stages of plaice, Pleuronectes platessa L, and Dover sole, Solea solea. 

Aquaculture 4: 41-53. 

 The response of the developmental stages tested was examined under constant 

flow conditions using direct electrolysis of sea water as a source of chlorine. From LC50

determinations, the eggs of plaice were found to be more tolerant than the newly-hatched 

larvae, and for both plaice and Dover sole the tolerance of the larvae increased as their 

development proceeded up to metamorphosis. Less change in tolerance was evident with 

increasing size of fish after metamorphosis. Determinations of time to kill 50% of a test 

population showed that at chlorine concentrations only slightly higher than LC50 level the 

time for survival was considerably reduced. Growth of the metamorphosed fish of both 

species at sublethal levels only showed a very marked depression at concentrations very 

close to the LC50 level. There was some evidence of acclimation to chlorine during long 

exposure. The growth of fish in power station effluents should not be affected by the use 

of low level chlorination as an anti-fouling measure, since the chlorine is normally 

rapidly absorbed by reactions taking place in the sea water. Large fluctuations in the 

chlorine dose could however present a hazard. 

Allonier, A.S. and M. Khalanski. 1998. Sea water chlorination by products. Journal. 

Research Oceanography 23(1): 21-24. 

 A wide range of methods have been developed to control biofouling in nuclear 

power plant cooling circuits using sea water; of these, low level chlorination is still the 

most commonly used. The oxidizing compounds generated by the chlorination of sea 

water are mainly hypobromous acid and bromamines when ammonia nitrogen is present. 

These compounds rapidly disappear from the water after its discharge in coastal waters. 

However, the organohalogenated (OX), mainly brominated compounds are more 

persistent. The volatile components of OX can easily be measured by gas 

chromatography (GC); bromoform is the predominant volatile product found in 

chlorinated sea water. Less volatile compounds, such as haloacetonitriles, halophenols 

and haloacetic acids, can also be measured by GC after liquid liquid extraction. Global 

measurement of OX extracted in an organic solvent (extractable organohalogenated 

compounds or EOX) by means of microcoulometry is also possible. The kinetics of 

bromoform formation and bromoform formation yields have been experimentally 

measured in sea water samples from nuclear power stations located on the English 

Channel coast in France: Gravelines, Penly and Paluel. Bromoform is rapidly generated 

in the chlorination range of 0.5 mg/L to 4.0 mg/L, with a molar yield of less than 1%. 

Field GC measurements carried out on chlorinated cooling waters at several power 

stations in France have confirmed. The experimental findings: dibromoacetonitrile and 

tribromophenol were found at lower concentrations than bromoform. However, the EOX 

method is not suitable for quantitative measurements of OX at low levels (<5 μg/L). The 

bromoform input in coastal waters due to chlorination at nuclear power stations is 

compared to estimations of the natural input of bromoform in sea water. 

10 
Allonier, A.S., M. Khalanski, V. Camel and A. Bermond. 1999. Characterization of 

chlorination by-products in cooling effluents of coastal nuclear power stations. Marine 

Pollution Bulletin 38(12): 1232-1241. 

 In power stations, the cooling effluents are chlorinated to avoid excessive 

biofouling. Yet, this disinfecting treatment leads to the formation of halogenated byproducts. In spite of possible toxicity of such compounds towards aquatic organisms, 

there is an evident lack of information on the formation of halogenated compounds in 

chlorinated seawater samples. So, this study was undertaken to identify and quantify 

halogenated compounds (i.e., trihalomethanes, haloacetonitriles, halophenols and 

haloacetic acids) in effluents from three French nuclear power stations. In addition, 

experiments conducted in the laboratory allowed the determination of the kinetics of 

formation of the main compounds detected: bromoform, dihaloacetonitrile, 2,4,6-

tribromophenol and dibromoacetic acid. Good agreement was obtained between the 

experiments conducted either on site or in the laboratory. In particular, evidence was 

given on the formation of brominated compounds (due to the high concentrations of 

bromide ions in seawater). 

Amy, G., M. Siddiqui, K. Ozekin, H.W. Zhu and C.Wang. 1998. Empirically based 

model for predicting chlorination and ozonation by-products: trihalomethanes, haloacetic 

acids, chloral hydrate, and bromate. Washington, DC, Environmental Protection Agency 

Office of Water. 172 pp. 

 This report documents a series of statistically-based empirical models for use in 

predicting disinfection by-products (DBPs) formed during water treatment disinfection 

using chlorine or ozone. Each model was formulated through multiple step-wise 

regression analysis, and as such takes the form of a multiple regression equation. After 

formulation and calibration, model simulations were performed to compare predicted 

versus measured values, employing the same data base used in model calibration. 

Finally, each model was validated by using data derived from the literature. The relevant 

chlorination DBPs include haloaetic acids (HAAs), trihalomethanes (THMs), and chloral 

hydrate (CH). Bromate (BrO3-) served as the ozonation DBP of interest. For both 

chlorination and ozonation, dissolved organic carbon (DOC) has been used to represent 

the organic DBP precursor, while bromide ion (Br-) represents the inorganic precursor. 

For the chlorination DBPs, model variables includes water quality conditions; DOC, Br-, 

pH, and temperature; and treatment conditions represented by chlorine dose and reaction 

time. The models permit prediction of total HAAs (within the context of this report, total 

HAAs, THAA, corresponds to sum of six species, HAA6), total THMs, and CH as a 

function of time; supplementary models permit estimates of each of the four THM 

species, and each of six HAA species. 

Anderson, C.D., D.J. Brown, B.A. Ketschke, E.M. Elliot and P.L. Pike. 1975. The 

effects of the addition of a fourth generating unit at the Salem Harbor generating station 

on the marine ecosystem at Salem Harbor. Report, Division of Marine Fisheries, 

Department of Fisheries, Commonwealth of Massachusetts, Boston, MA. 

 No abstract. 

11 
Anderson, D.R., R.M. Bean and C.I. Gibson. 1979. Biocide by-products in aquatic 

environments Annual report, October 1, 1977-September 30, 1978. NUREG/CR-0504; 

PNL-2806 DOE. Richland, WA (USA), Battelle Pacific Northwest Labs. 67 pp. 

 The Biocide By-Products in Aquatic Environments Program is composed of 

analytical chemistry and Biological phases with freshwater and marine Biological 

subdivisions. The objectives of the analytical studies are: to identify those chloroorganic 

chemical compounds that result from the addition of chlorine to fresh or saltwater; to 

develop methods for detecting chlorinated organics in the effluents discharged to 

receiving water bodies from nuclear stations; and to verify laboratory findings through 

analysis for chlorination by-products in water and biota samples from cooling water 

bodies of nuclear power stations. The objectives of the Biological studies are: to 

investigate the immediate toxicity of specific chlorination by-products (chloroform in 

freshwater and bromoform in marine waters); to evaluate the chronic toxicity of 

chlorination by-products; to follow their pathways of action; and to analyze for 

bioaccumulation or biomagnification of halogenated hydrocarbons on selected aquatic or 

marine biota. 

Anderson, D.R., R.M. Bean, C.I. Gibson and T.O. Thatcher. 1978. Synthesis, pathways, 

effects, and fate of chlorination by-products in freshwater, estuarine and marine 

environments. Annual report, September 10, 1976-September 30, 1977. PNL-2909 

DOE. DOE. Richland, WA (USA), Battelle Pacific Northwest Labs. 86 pp. 

 The study is composed of analytical chemistry and Biological divisions with 

freshwater and marine Biological subdivisions. The objective of the analytical phase is to 

identify those chemical compounds, other than the free and combined available halogen, 

which result from the addition of chlorine to fresh or saltwater. The objectives of the 

Biological studies are to investigate the immediate and relatively long-term toxicity of 

several chlorination by-products to selected aquatic biota; to follow their pathways of 

action; and to analyze for bioaccumulation or biomagnification. Initial analytical 

experimentation has been directed toward isolating and identifying nonpolar, lipophylic 

organohalogens that might be expected to be absorbed and biomagnified in the lipids of 

aquatic biota. Chlorinated natural fresh and marine water samples were obtained from 

Task IIa and b. Organic components were concentrated by forcing chlorinated and 

unchlorinated water through columns of XAD-2 resin using a positive displacement 

pump. Ether extracts of the XAD-2 columns were analyzed for haloforms by gas 

chromatography. Bromoform was found to be the major constituent in all chlorinated sea 

water samples. In contrast, chloroform was the only haloform produced from chlorinated 

freshwater. The Freshwater Biology 6-month chronic bioassay on rainbow trout has 

produced several results. The mortality rate, although low, indicated an interesting trend 

in response to increasing concentration of chlorination by-products. In addition to 

observed mortality, concentration was inversely related to other fish losses which are 

hypothesized to result from decreased aggressiveness. Fish length and weight at six 

months was also significantly reduced with increasing concentrations of chlorination by12 
products. A chronic bioassay of chlorination by-products was conducted with little-neck 

clams in the marine phase of the program. 

Aprosi, G. 1988. Bryozoans in the cooling water circuits of a power plant. Proceedings 

of the International Association of Theoretical and Applied Limnology Conference. 

 The first aim of this study was to determine the conditions favoring the growth of 

colonies in the cooling water circuits. The following factors have been examined: growth 

rate, statoblast distribution, effects of temperature, light and water velocity. A quantity of 

approximately 120 x 106

 phytoplankton cell/L provided a flourishing growth and the 

trophic parameter was not a limiting factor in the Loire River. The second aim was to 

design the methods most suitable for controlling bryozoan proliferation. It was 

concluded that shock chlorination, water deoxygenation and some anti-fouling paints 

couldn't be used or were proved inefficient. 

Attrill, M., M. Power and R. Thomas. 1999. Modelling estuarine crustacea population 

fluctuations in response to physico-chemical trends. Marine Ecology Progress Series 

178: 89-99. 

 Regular samples (generally every 2 wk) of 6 estuarine crustacean species, 

Carcinus maenas, Liocarcinus holsatus, Crangon crangon, Palaemon longirostris, 

Palaemon serratus and Gammarus (mainly G. zaddachi), were taken over a 12 yr period 

from the cooling water intake screens of West Thurrock power station on the Thames 

estuary, UK. Additionally, comparative data sets for abiotic variables (freshwater flow, 

salinity, temperature, dissolved oxygen, pH, suspended solids, total nitrogen) were 

collected for the same time period. The comprehensive nature of the time series, and 

accompanying suite of variables, allowed the construction of statistical models for the 

trends in population abundance of the 6 species using multiple linear regression 

techniques. Statistically significant models were constructed for C. maenas, C. crangon

and Gammarus, accurately predicting annual and longer term, fluctuations in abundance. 

All models had strong seasonal components, although for C. maenas temperature was the 

only physico-chemical variable with significant explanatory power. The importance of 

temperature as a controlling variable for the species was reinforced by the inclusion of an 

instrumental variable to simulate a threshold temperature for foraging activity. The 

optimal value was found to be 8°C. C. crangon was found to be positively correlated 

with dissolved oxygen, but showed a slight decline in abundance over the time period. 

There was no significant relationship with either salinity or temperature variables 

previously suggested as being important. Gammarus abundance had 2 significant 

explanatory variables (temperature and salinity) but also demonstrated a large decrease in 

population size with time. L. holsatus and P. serratus are summer-occurring species, so 

were recorded too infrequently to adequately capture seasonal dynamics. Despite the 

long time series, no significant model was possible for P. longirostris abundance (nonnormal residuals), which has been suggested previously as having a strong relationship 

with salinity. The results of the study provide the first significant multiple linear 

regression models that accurately predict estuarine crustacean abundance. Whilst these 

models are useful for helping to understand variability in the Thames, it will be 

13 
interesting to determine whether populations in other estuaries demonstrate relationships 

with similar suites of physico-chemical parameters. 

Azanza, M., R. Azanza, A. Gedaria, H. Sententa and M. Idjao. 2001. Decimal reduction 

times of Pyrodinium bahamense var. compressum and Escherichia coli in chlorine- and 

ultraviolet-treated seawater. Letters in Applied Microbiology 33(5): 371-376. 

 Decimal reduction times (D-values) of the vegetative cells of Pyrodinium 

bahamense var. compressum and Escherichia coli in ultraviolet- and chlorine-treated 

seawater were established. Methods and Results: The cells of the test organisms were 

exposed to ultraviolet- and chlorine-treated seawater and maintained at 20-35 ppt salinity 

and 20 to 35°C. The dinoflagellate cells which cause Paralytic Shellfish Poisoning (PSP) 

were found to be more resilient than the bacterial cells. Ultraviolet treatment was found 

to be more effective than chlorine to both test organisms. Irreversible morphological 

changes in the treated dinoflagellate cells were noted, including protoplast discoloration, 

cellular membrane leakage and damage to the thecal armour. Conclusions: The 

vegetative cells of both test organisms in seawater were more sensitive to ultraviolet 

treatment than to chlorine exposure. Generally, the dinoflagellate cells were less 

susceptible than bacterial cells to both disinfection treatments. Significance and Impact 

of the Study: Results of this study may have significant implications in depuration 

procedures for mollusks and cleaning protocols for ballast waters of ships. 

Baier, R., A. Meyer and R. Forsberg. 1994. Controlling the impact of zebra mussels on 

estuarine flow control/filtration structures by nontoxic, easy-release coatings. IAGLR. 

166 pp. 

 Small, inland hydroelectric plants on Lake Ontario estuaries have been seriously 

impacted by accumulations of zebra mussels (Dreissena polymorpha) on their flow 

control/filtration structures--particularly trashracks--as well as the inplant turbines and 

housings. Using a series of test devices, ranging from small flow-controlled manifolds to 

full field-deployed coated trash racks, at Lake Glenwood (combining flows from Oak 

Orchard Creek, the Erie Barge Canal, and other sources), Medina, NY, it was 

demonstrated that maintenance of coating "critical surface tension" in the zone between 

20 and 30 dynes/cm correlated with easy physical (hand wiping or water lance) removal 

of zebra mussels at the level of their byssus disc bases, leaving the structures clean and 

free of obstructions. On the other hand, materials with higher critical surface tensions 

required water lance pressures of more than 1, 500 psi to clean them, and this was 

achieved only by breaking the zebra mussel byssus threads to leave behind a "fuzzy, " 

debris-catching layer that was easily re-fouled. The most successful coatings are 

compliant, elastomeric silicones which have shown surprising toughness and abrasion 

resistance in both laboratory tests and field trials. Use of this nonpolluting control 

technology, based on understanding the interfacial biophysics of the zebra mussel 

attachment mechanism, can minimize the requirements for chlorination, thermal 

treatments, or poisonous paints in multi-use estuarine systems. Support from New York 

Sea Grant Institute, Niagara Mohawk Power Corporation, King Consulting, Inc., and 

Gilbert/Commonwealth, Inc. is gratefully acknowledged (DBO). 

14 
Bamber, R.N. and R.M.H. Seaby. 2004. The effects of power station enrichment 

passage on three species of marine planktonic crustacean, Acartia tonsa (Copepoda), 

Cragnon cragnon (Decapoda) and Homarus gammarus (Decapoda). Marine 

Environmental Research 57(4): 281-294. 

 Experiments have been undertaken exposing larval common shrimp (Crangon 

crangon) and lobster (Homarus gammarus) and adult copepods (Acartia tonsa) to the key 

stresses of entrainment within power-station cooling-water systems. The apparatus has 

enabled the testing of mechanical, thermal, chlorine and realistic pressure effects both 

alone and in combination, the range of stressors spanning the standard conditions found 

within a temperate coastal direct-cooled power station. Mechanical stresses affected only 

lobster larvae, pressure changes affected only the Acartia adults. Residual chlorine 

caused significant mortality of Acartia and shrimp larvae, but had no effect on lobster 

larvae even at 1 ppm. The temperature increment significantly affected all three species, 

with a synergistic effect on chlorine sensitivity in the shrimp larvae, but only 

temperatures higher than would be experienced in a normally operating power station 

affected the copepods. The majority of individuals of each species would survive 

passage through a power-station system under normal conditions. It is notable that, 

within the species tested, generalizations from the responses of one species to those of 

another are not valid. 

Beauchamp, R.S.A. 1969. The use of chlorine in the cooling water system of a coastal 

power station. Chesapeake Science 10: 280. 

 In the intake conduits of coastal power stations marine fouling may build up to a 

remarkable extent. In two extreme cases the amount of marine fouling, consisting of 

barnacles, tube worms, mussels and debris, has exceeded 300 tons. The greatest hazard 

to station operation arises from the growth of mussels as these grow to a size that they 

can, when detached, block or partially block condenser tubes. The schedule now being 

used is to inject chlorine continuously at a rate of 0.5 ppm. It seems that combined 

chlorine or combined bromine, probably in the form of bromamines, is extremely 

distasteful to mussels. Bacterial slime that forms in the condensers can be controlled by 

injecting chlorine at 2-3 ppm for 15 minutes every 6 hours. The adoption of a schedule 

of low level chlorination for the control of marine fouling has made it possible to carry 

out trials on the farming of sea fishes using the warm water discharged from power 

stations. 

Behrens, D.W. and B.C. Larsson. 1976. Effects of intermittent chlorination on selected 

invertebrate species indigenous to Diablo Cove- a laboratory study. Report No. 7846. 7-

76. 

 No abstract. 

15 
Bellanca, M.A. and D.S. Bailey. 1977. Effects of chlorinated effluents on aquatic 

ecosystems in the lower James River. Journal Water Pollution Control Federation 49: 

639-645. 

 The effects of chlorinated wastewater effluents on aquatic life were studied after 

massive fish mortalities occurred in the lower James River during the spring of 1973. 

Through the utilization of field bioassays, live box experiments, and laboratory toxicity 

tests, it was determined that chlorine and chloramines were acutely toxic to a wide 

variety of estuarine animals. Toxicity symptoms noted for fish included the occurrence 

of severely dislocated and broken vertebrae. Studies were also conducted on the 

relationship of fecal coliform levels in the receiving stream and chlorination at 

wastewater treatment plants. It was found that chlorine dosage rates at the wastewater 

treatment plants could be reduced significantly without an adverse effect on bacterial 

levels in the estuarine system. 

Benanou, D., F. Acobas and P. Sztajnbok. 1998. Analysis of haloacetic acids in water 

by a novel technique: Simultaneous extraction-derivatization. Water Research 32(9): 

2798-2806. 

 A simple and novel method has been developed for the analysis of haloacetic 

acids (HAAs), disinfection by-products (DBPs) formed during water chlorination. This 

method can be used to determine six of these acids, including chloro, bromo and 

chloro/bromo acids. The validity and reliability of the method were tested over 6 months 

on twenty French water samples. 50 ml of water are percolated over an ion-exchange 

resin, the strong acids are trapped and then eluted and simultaneously esterified to give 

their methyl derivatives by a methyl alcohol solution acidified with sulfuric acid. The 

esters are extracted by cyclohexane and analyzed by gas chromatography with electroncapture detection (GC/ECD). Dalapon is used as a tracer in order to monitor the 

extraction process and trichloropropane as the internal standard. The detection limits for 

the HAAs analyzed are between 0.1 μg/l and 0.2 μg/l. The analysis of different water 

samples showed that all the haloacetic acids, except monobromoacetic acid, were present. 

The total HAAs in water originating from surface waters was less than 50 μg/l, but could 

exceed 100 μg/l for water originating from reservoir waters. In underground waters, the 

concentration of HAAs was negligible. 

Bender, M.E., M.H. Roberts, R. Diaz and R.J Huggett. 1977. Effects of residual chlorine 

on estuarine organisms. Pp. 101-108. In: L.D Jensen (ed). Biofouling Control 

Procedures Technology and Ecological Effects. Marcel Dekker, Inc., New York, NY. 

 No abstract. 

Bidwell, J.R., D.S. Cherry, J.L. Farris, J.C. Petrille and L.A. Lyons. 1999. Effects of 

intermittent halogenation on settlement, survival and growth of the zebra mussel, 

Dreissena polymorpha. Hydrobiologia 394(1-3): 53-62. 

 The effect of intermittent (2-4 h/day) treatments with chlorine or bromine at levels 

of 0.5 and 1.0 mg/L (total residual oxidant) upon settling and growth of veliger larvae of 

16 
the zebra mussel, Dreissena polymorpha, was examined in two successive flow-through 

studies. The work was conducted in a field laboratory which received a constant supply 

of water from western Lake Erie. Veliger densities in the water at the field site peaked at 

530/L, while mussel densities on settling monitors reached 147,100/m2

 over the course of 

the two studies (early July to late September 1991). In Study 1, a 2-h daily treatment 

with 1.0 mg/L chlorine reduced mussel settling by 91% as compared with controls, 

although mussel densities of up to 6,000/m2

 still occurred. Treatment with 0.5 mg/L 

chlorine for 4 h/day produced a similar reduction in mussel settling during the second 

study. Bromine was less effective than chlorine at reducing settling of veligers. Mussels 

which remained settled in the treatment tanks had growth rates similar to controls, 

reaching 2-4 mm in length over a 30-day period. The intermittent halogen treatments 

similarly had no effect on whole body glycogen levels or growth of adult D. polymorpha. 

The intermittent chlorination regime used may therefore delay, but would not prevent a 

mussel fouling problem. 

Blatchley, E.R. and Y. Xie. 1995. Disinfection and antimicrobial processes. Water 

Environment Research 67(4): 475-481. 

 Recent and pending amendments to water and wastewater disinfection policies are 

likely to bring about changes in disinfection processes. Many state regulatory agencies 

have incorporated stringent residual chlorine limitations on wastewater effluents. 

Ultraviolet radiation has been tested to replace some other means of disinfection in many 

waters and wastewaters. Ozone represents another alternative to halogen disinfection 

which has undergone extensive evaluation. Halogen-based applications remain the most 

frequently applied disinfection processes because of the relatively advanced state of 

knowledge regarding halogen systems and favorable economic considerations. Several 

other processes within water and wastewater treatment systems are effective in removal 

of pathogenic microorganisms. Most of these include some type of media filter to 

remove pathogens. Several techniques are available for the identification of pathogens in 

water. These methods help to determine the quality of the final water product after 

disinfection. Photolysis can break down free chlorine and affect the amount of residual 

chlorine. On-site generation of chlorine-based disinfectants by electrolysis of NaCl 

solutions is increasing. The formation of byproducts of disinfection, their toxicity and 

control has been extensively reviewed. Disinfection byproducts found in swimming 

pools included trihalomethanes, acetonitriles, chloral hydrate, and halogenated organic 

acids. Some disinfection byproducts were found to be mutagenic. Other treatment 

processes for the removal of bacterial cells include adsorption onto insoluble copolymer 

beads, ion exchange resins, ion inactivation, gamma irradiation, and electrochemical 

inactivation. The attachment of bacteria to surfaces during disinfection and biofilm 

formation may affect the disinfection process. 

Block, R.M. 1977. Physiological responses of estuarine organisms to chlorine. 

Chesapeake Science 18: 158-160. 

 Chlorine produced stress to estuarine organisms may be indicated by 

physiological responses that are not reflected in mortality studies. The physiological 

17 
parameters selected should relate to the functional physiology for a particular species. 

Time of exposure should be correlated with the behavior patterns for the test species. 

More research is required using multivariate design on the effects of chlorine on selected 

euryhaline species at various salinities. 

Block, R.M., J.C. Rhoderick and S.R. Gullans. 1977. Physiological responses of white 

perch (Morone americana) to chlorine. Association Southeastern Biologists Bulletin 24: 

37. 

 No abstract. 

Block, R.M., D.T. Burton, S.R. Gullans and L.B. Richardson. 1978. Respiratory and 

osmoregulatory responses of white perch (Morone americana) exposed to chlorine and 

ozone in estuarine waters. Pp. 351-360. In: Jolley, R.L., H. Gorchev and D.H. Hamilton 

(eds). Water Chlorination Environmental Impact and Health Effects Vol. 2 Ann Arbor 

Science Publishers, Inc., Ann Arbor, MI. 

 No abstract. 

Bolster, C.H., J.M. Bromley and S.H. Jones. 2005. Recovery of chlorine-exposed 

Escherichia coli in estuarine microcosms. Environmental Science & Technology 39(9): 

3083-3089. 

 Laboratory microcosm experiments were performed to determine whether 

chlorine-exposed Escherichia coli are capable of recovery (i.e., increase in numbers of 

culturable cells) in estuarine waters and if so what water-quality parameters are 

responsible for this recovery. Suspensions of E. coli were exposed to 0.5 mg/L of 

chlorine for 5 minutes followed by dechlorination with sodium thiosulfate. The chlorineexposed bacteria were introduced into 2-L microcosms containing estuarine water 

collected from the seacoast region of New Hampshire. Culturable cells in the 

microcosms were enumerated at 0, 10, 24, 48, and 74 h. In all estuarine microcosms the 

number of culturable cells increased by factors ranging from 2.8 to 50 over the 74-h 

incubation period. Multiple linear regression analyses indicated that ammonium and 

salinity were most significantly correlated with the recovery of E. coli over the 74-h 

incubation period; however, ammonium concentrations were strongly correlated with 

dissolved organic carbon and total dissolved nitrogen, making it impossible to determine 

with any degree of certainty the unique effect nitrogen or carbon had on recovery. The 

extensive recovery observed in our study indicates that following exposure to 

concentrations of chlorine that cause cell injury rather than death, numbers of culturable 

E. coli may increase significantly when discharged into estuarine waters. Thus, 

depending on the effectiveness of the chlorination process, the regular monitoring of 

chlorinated wastewater treatment effluent may underestimate the true impact on waterquality and public health risks. 

18 
Bongers, L.H., B. Bradley, D.T. Burton, A.F. Holland and L.H. Liden. 1977. Biotoxicity 

of bromine chloride and chlorine treated power plant condenser cooling water effluent. 

Report by Martin Marietta Corporation. 

 The dechlorination of chlorinated sewage effluent was studied to demonstrate that 

such a process would effectively eliminate toxicity attributable to the chlorine produced 

oxidants (CPO). The design of the study stipulated that the simulated effluent chlorine 

concentration should be the same as that in an actual plant effluent, 2 mg/L, as occurs at 

the James River Sewage Treatment Plant. A portion of the chlorinated sewage effluent 

was dechlorinated with sodium thiosulfate, and the dechlorinated waste was diluted with 

estuarine water in the same proportions as the chlorinated waste. Menidia menidia, 

Palaemonetes pugio, and Crassostrea virginica were exposed to both the chlorinated and 

dechlorinated wastewater. Mortalities were noted in M. menidia and P. pugio due to high 

concentrations of chlorinated effluent, whereas similar concentrations of dechlorinated 

wastes did not cause mortalities. Mortalities in the chlorinated waste were correlated 

with chlorine residuals. The chlorinated wastes depressed the shell deposition rate of C. 

virginica below 50% of controls at all doses. Dechlorinated waste allowed shell 

deposition greater than 50% of controls. The occurrence of some growth inhibition, 

however, was noted in C. virginica exposed to dechlorinated waste, which suggests the 

presence of toxic material other than chlorine in the waste. 

Bradley, B.P. 1978. Comparison of residual biotoxicity of chlorine and bromine 

chloride to copepods. Technical Report No. 47, University of Maryland, Water 

Resources Research Center, College Park, MD. 

 No abstract. 

Brook, A.J. and A.L. Baker. 1972. Chlorination at power plants: impact on 

phytoplankton productivity. Science 176 (4042): 1414-1415. 

 Studies of the effects of passage through a power plant on river phytoplankton 

have shown that chlorination depresses rates of photosynthesis and respiration to a much 

greater extent than does heating. 

Brooks, A.S. 1974. Phytoplankton entrainment studies at the Indian River estuary, 

Delaware. Pp. 105-111. In: L.D Jensen (ed). Proceedings of the Second Workshop on 

Entrainment and Intake Screening. The Johns Hopkins University, Department of 

Geography and Environmental Engineering, Baltimore, MD. 

 No abstract. 

Browdy, C.L., T. Smyth and D. Bratvold. 1998. Preliminary development of a biosecure 

shrimp production system. Aquaculture '98 Book of Abstracts. P. 71. 

The US Marine Shrimp Farming Program has developed fast growing, high health 

and genetically improved shrimp stocks, disease diagnosis and control protocols, and 

environmentally friendly growout technologies to provide a basis for the profitable 

19 
controlled production of healthy shrimp. Nevertheless, outbreaks of viral disease 

continue to cause crop failures in Texas and South Carolina. The present study explores 

strategies for more biosecure growout technologies. The tested system reduced the 

likelihood of pathogen contamination by disinfection of intake water and physical 

exclusion of some possible vectors which could carry disease. A greenhouse structure 

erected over a 0.1 ha pond was covered with a plastic roof and walls of fine mesh shade 

cloth to allow air exchange. The sediment was dried and limed. A 5.5% bleach solution 

was poured over a small section of the sediment near the drain that did not dry 

completely. Incoming seawater was disinfected with chlorine at a CT of 1200 to 2000 

min x ppm by pumping sand filtered seawater and a concentrated chlorine solution into a 

raceway contact chamber and then into the pond over a nine-day period. Disinfection 

success was assessed by colony forming units (CFU) on nutrient media and direct 

bacterial counts by fluorescence microscopy. At least 99% of the bacteria and yeast 

present in the filtered seawater were destroyed by this disinfection procedure. However, 

CFU were abundant on all plates from the sediment site, indicating a lack of sediment 

disinfection, despite significant residual chlorine in the overlying water column, and 

likely contamination of the interstitial water with water column water during the 

sampling process. These results suggest the difficulty in achieving sediment disinfection, 

however, destruction of pathogens may not require complete disinfection. The microbial 

community was monitored throughout the season. The following parameters were 

measured weekly: bacterial abundance, phytoplankton and zooplankton abundance and 

biomass, oxygen consumption and nitrification rates, and total ammonia concentration. 

Chlorinated water additions ceased on Day 9, and residual chlorine concentrations 

dropped rapidly. This was followed by a sharp increase in water column bacterial 

abundance. Two days after residual chlorine reached undetectable levels, the pond was 

fertilized and inoculated with a pure culture of Chaetoceros gracilis (Day 13). The initial 

modest C. gracilis bloom (Day 16) was followed by a large bacterial bloom and then a 

large mixed algal bloom (Day 30), of which C. gracilis was a small component. The 

initial very large fluctuations in phytoplankton and appeared to reach more normal 

fluctuation levels by Day 50. The initial bloom and stabilization of nitrifying bacteria 

lagged about 20 days behind the other measured groups, making nitrifiers the last group 

to stabilize. The biosecure pond was stocked at a density of 100/M2

 on Day 16. Survival 

was 70%, mean size at harvest was 13g, for a production rate of 9109 kg/ha/crop. 

Feeding trays were used to monitor consumption but feed was offered at a constant rate 

from stocking to harvest. Overall the food conversion ratio was 2.2:1. The study 

provided hands on experience in designing and managing a biosecure production strategy 

providing useful basic information for future development efforts. 

Brungs, W.A. 1973. Effects of residual chlorine on aquatic life. Journal of Water 

Pollution Control Federation 45(10): 2180-2193. 

 Increased use of Cl and recent studies of residual Cl toxicity in aquatic systems 

have emphasized the need for close scrutiny of present disinfection procedure. This 

review discusses Cl uses and Cl chemistry and emphasizes toxicity studies in the field 

and in the lab. Interim criteria, based on knowledge to date, for permissible concs of total 

residual Cl are: (a) in areas receiving wastes treated continuously with Cl, not to exceed 

20 
0.01 mg/L for the protection of more resistant organisms only, or not to exceed 0.002 

mg/L for the protection of most aquatic organisms; and (b) in areas receiving 

intermittently chlorinated wastes, not to exceed 0.2 mg/L for a period of 2 hr /day for 

more resistant spp of fish, or not to exceed 0.04 mg/L for a period of 2 hr/day for trout 

and salmon. If free Cl persists, more restrictive criteria are warranted. Alternate 

procedures or substitutes for chlorination should be investigated. 

Buckley, J.A. 1976. Acute toxicity of residual chlorine in wastewater to coho salmon 

(Oncorhynchus kisutch) and some resultant hematological changes. Journal of Fisheries 

Research Board Canada. 33: 2854-2856. 

 Juvenile coho salmon were exposed to treated wastewater containing lethal levels 

of total residual chlorine (TRCl2) with seawater diluents under continuous flow 

conditions. The 96-h TL50 [tolerance limit to 50% of test fish] was 0.07 mg/L TRCl2 

(4% effluent). Exposure resulted in a slight reduction (significant P = .05) in Hb, a slight 

increase (significant P = .05) in methemoglobin, and a temporary decrease (not 

significant P = .05) in percentage of circulating immature erythrocytes. 

Buckley, J.A. 1977. Heinz body hemolytic anemia in coho salmon (Oncorhynchus 

kisutch) exposed to chlorinated seawater. Journal Fisheries Research Board Canada. 34: 

215-224. 

 Fingerling coho salmon (O. kisutch) were exposed for 12 wk to various 

concentrations of treated wastewater contaning total residual chlorine (TRCl2) with 

riverwater diluent under continuous flow conditions. Levels of TRCl2 averaging 0.003-

0.05 mg/L resulted in mild to pronounced symptoms of hemolytic anemia, including an 

increase in numbers of circulating immature erythrocytes, pathological changes in 

erythrocytes, and a reduction in packed cell volume and Hb levels. In addition, Heinz 

bodies were formed at the 0.012 and 0.05 mg/L TRCl2 levels. The hematologic changes 

were attributed to the oxidative nature of TRCl2. 

Buckley, J.A. and R.I. Matsuda. 1972. Toxicity of the West Point treatment plant 

effluent to coho salmon, Oncorhynchus kisutch. Report, Municipality of Metropolitan 

Seattle, WA. 

 No abstract. 

Buckley, J.A. and R.I. Matsuda. 1973. Toxicity of the Renton treatment plant effluent to 

coho salmon, Oncorhynchus kisutch. Report, Municipality of Metropolitan Seattle, WA. 

 No abstract. 

21 
Buckley, J.A., C.M. Whitmore and R.I. Matsuda. 1976. Changes in blood chemistry and 

blood cell morphology in coho salmon (Oncorhynchus kisutch) following exposure to 

sublethal levels of total residual chlorine in municipal wastewater. Journal Fisheries. 

Research Board Canada 33: 776-782. 

 Yearling coho salmon (O. kisutch) were exposed for 12 wk to either 0.3, 1.1 or 

3.6% chlorinated municipal sewage treatment plant effluent with seawater diluent 

(average salinity 28%) under continuous flow conditions. The maximum safe 

concentration of effluent lies between 0.3-1.1% (average total residual chlorine [TRCl2] 

content 0.003 and 0.009 mg/L, respectively). Effluent concentration of 0.3% produced 

no discernible effects on the fish. Concentrations of 1.1 and 3.6% (average content of 

TRCl2 0.030 mg/L) resulted in reductions of Hb and hematocrit to levels indicative of 

anemia. Observations of the erythrocytes revealed lysed and degenerating cells, 

increased numbers of circulating immature cells and abnormal cells. These 

hematological effects are attributed to the oxidative nature of TRCl2. 

Burton, D.T. and S.L. Margrey. 1979. Control of fouling organisms in estuarine cooling 

water systems by chlorine and bromine chloride. Environmental Science Technology 

13(6): 684-689. 

 The relative antifouling effectiveness of chlorine and bromine chloride under 

intermittent and continuous modes of application in low velocity flow areas was 

evaluated at an estuarine power plant located on the Chesapeake Bay. No significant 

difference in the control of fouling organisms was found on the average between similar 

concentrations of chlorine and bromine chloride. Significant differences in fouling were 

found between intermittent and continuous halogenation on both clean and prefouled 

surfaces. Continuous halogenation was more effective over an entire fouling season in 

controlling primary, secondary, and adventitious fouling communities than intermittent 

halogenation for periods up to 2 h/day. Continuous halogenation at 0.3 mg/L total 

residual halogen was more effective than 0.1 mg/L total residual halogen during late 

spring and summer; no difference was found between the 2 concentrations during the 

early fall. 

Burton, D.T. and D.J. Fisher. 2001. Chlorine dioxide: the state of science, regulatory, 

environmental issues, and case histories. Wye Research and Education Center Report: 16 

pp. 

 The use of chlorine by electric utilities and other surface water users to inhibit 

biofouling and the chlorination of wastewater by POTWs to eliminate the discharge of 

pathogenic organisms are widespread practices. A number of surface water users in the 

Great Lakes region recently expressed an interest in using chlorine to control the zebra 

mussel (Dreissena polymorpha) which was introduced from Europe in the mid-1980s. It 

is well known, however, that chlorine-produced oxidants may be toxic to aquatic life 

when discharged into receiving waters. In addition, chlorine reacts with ammonia and 

chlorinated hydrocarbons to form various chloramines and trihalomethanes, which have 

long half-lives and similar toxicities relative to free chlorine. 

22 
Burton, D.T., L.W.J. Hall and S.L. Margrey. 1979a. Interactions of chlorine, 

temperature change and exposure time on survival of striped bass (Morone saxatilis) eggs 

and prolarvae. Journal Fisheries Research Board Canada 36: 1108-1113. 

 Effects of total residual chlorine (TRC), change in temperature (ΔT) and exposure 

time were established for eggs and prolarvae of striped bass, M. saxatilis, under test 

conditions designed to simulate entrainment by power plants. Prolarvae were more 

sensitive than eggs under the same test conditions. In contrast to the predominant effect 

of TRC and exposure time on eggs, TRC, ΔT and exposure time were all important in 

causing mortality of prolarvae. [A predictive mortality model was discussed.] In an 

evaluation of the potential effects of chlorinated power plant cooling waters to striped 

bass ichthyoplankton, a systematic study must be made of the interactions of TRC, ΔT 

and exposure time. 

Burton, D.T., L.W. Hall and S.L. Margrey. 1979b. Multifactorial chlorine, ΔT and 

exposure duration studies of power plant effluents on esturarine ichthyoplankton. MidAtlantic Industrial Wastes Conference. Pp. 223-229. 

 No abstract. 

Cakiroglu, C. and C. Yurteri. 1998. Methodology for predicting cooling water effects on 

fish. Journal of Environmental Engineering 124(7): 612-618. 

 The mathematical model presented here predicts the long-term effects of oncethrough cooling water systems on local fish populations. The fish life cycle model 

simulates different life stages of fish by using appropriate expressions representing 

growth. All of abstract. 

Capuzzo, J.M. 1977. The effects of free chlorine and chloramines on growth and 

respiration rates of larval lobsters (Homarus americanus). Water Research 11: 1011-

1024. 

 The length, dry weight and standard respiration rate of larval lobsters (H. 

americanus) were monitored for 19 days following a 60 minute exposure at 25°C to 1.0 

mg/L applied free Cl and 1.0 mg/L applied chloramine. Compared to control organisms, 

significantly lower increases in dry weight (P < 0.05) and significant reductions in 

standard respiration rates (P < 0.01) were measured among exposed organisms; greater 

differences were detected among chloramine exposed organisms. Acute exposure to free 

Cl or chloramine results in subsequent reductions in growth and metabolic activity of 

larval lobsters. 

Capuzzo, J.M. 1980. Impact of power-plant discharges on marine zooplankton: A 

review of thermal, mechanical and biocidal effects. Helgolander Meeresuntersuchungen. 

European Marine Biology Symposium, Helgoland (FRG), 23 Sep 1979. 

23 
 The relative importance of thermal, mechanical and biocidal stresses to marine 

zooplankton entrained in cooling waters from coastal power-plant operations is 

dependent on specific features of power-plant design and siting. Toxic effects of powerplant operations will vary with (1) the degree of mechanical stress induced by pumping 

velocities of cooling water; (2) the physical and chemical interaction of receiving and 

discharge waters; (3) the dosage of chlorine or other biocide added to cooling waters for 

fouling control: (4) the exposure time to stress condition experienced during passage 

through condenser conduits and discharge canals and (5) the nature of receiving waters, 

affecting the production and availability of the various halogen toxicants formed upon 

chlorination of seawater. Because of these variables, the problem of entrainment-induced 

mortality of zooplankton and the resulting effects on secondary production in receiving 

waters is difficult to assess. 

Capuzzo, J.M., S.A. Lawrence, J.A. Davidson and M. Libni. 1976a. The differential 

effects of free and combined chlorine on juvenile marine fish. Estuarine Coastal Marine 

Science 5: 733-741. 

 The differential effects of free chlorine and chloramine on 3 spp. of juvenile 

marine fish were investigated in continuous flow bioassay units. The toxicity of both 

chlorine forms to winter flounder, Pseudopleuronectes americanus, scup, Stenotomus

versicolor and killifish, Fundulus heteroclitus, appeared to be a threshold effect: an 

abrupt increase in mortality was observed over a narrow range of toxicant concentrations. 

The 3 spp. were similar in their responses to free chlorine, the more toxic of the 2 

chlorine forms. There was a difference in chloramine toxicity among the 3 spp.; killifish 

were more susceptible than either of the other 2 spp., probably reflecting differences in 

metabolic regulation or uptake rates. Behavioral aberrations, distended gills and erratic 

swimming behavior, of winter flounder and scup and significant reductions in standard 

respiration rates of killifish were observed with exposure to chlorine or chloramine 

concentrations approaching lethal levels. 

Capuzzo, J.M., S.A. Lawrence and J.A. Davidson. 1976b. Combined toxicity of free 

chlorine, chloramines and temperature to stage I larvae of the American lobster Homarus 

americanus. Water Research 10: 1093-1099. 

 The differential effects of free Cl and chloramine on stage I larvae of H. 

americanus were investigated in continuous flow bioassay units. Applied chloramine 

was more toxic than corresponding concentrations of applied free Cl to lobster larvae 

with estimated LC50 values at 25°C of 16.30 mg/L applied free Cl and 2.02 mg/L applied 

chloramine. The synergistic effect of temperature on the toxicity of both free Cl and 

chloramine was also demonstrated. Exposure to applied free Cl at 20°C resulted in no 

significant mortality of test organisms, whereas exposure at 30° resulted in an estimated 

LC50 value of 250 mg/L. Applied chloramine was considerably more toxic with an 

estimated LC50 value at 20° of 4.08 mg/L and at 30° of 0.56 mg/L. The action of each 

toxicant appeared to be an alteration of standard metabolic activity as revealed by 

changes in respiration rates during and after exposure to applied free Cl and chloramine. 

Initial respiratory stress was detected during exposure to 0.05 mg/L applied chloramine 

24 
and 5.00 mg/L applied free Cl. Reductions in respiration rates 48 h after exposure were 

observed with exposure to all concentrations tested, similar results being obtained 

following exposure to 0.05 mg/L applied chloramine and 0.10 mg/L applied free Cl. 

These results are indicative of the need for information in addition to that obtained in 

standard bioassays for an adequate assessment of Cl toxicity. The apparent Cl demand of 

the seawater used in this study was determined after removal of particulate and dissolved 

organics and ammonia. Approximately 18% of the applied level of free Cl and 

chloramine was recovered as residuals, measured by amperometric titration; no reason for 

this low recovery was determined. Until it has been established that undetected Cl and 

chloramine in seawater do not result in the production of toxic compounds, both applied 

and residual levels should be reported in toxicity studies. 

Capuzzo, J.M., J.C. Goldman, S.A. Lawrence and J.A. Davidson. 1977. Chlorinated 

cooling water in the marine environment: development of effluent guidelines. Marine 

Pollution Bulletin 8: 161-162. 

 The effects of free chlorine and chloramine on stage I lobster larvae and juvenile 

killifish were investigated in continuous flow bioassay units. In comparing mortality and 

changes in standard respiration rates during and after exposure to either chlorine form, 

significant respiratory stress was observed with exposure to sublethal levels. Sublethal 

responses to free and combined chlorine should be considered when establishing 

regulations for chlorine residuals in cooling waters. 

Carpenter, E., B.B. Peck and S.J. Anderson. 1972. Cooling water and productivity of 

entrained phytoplankton. Marine Biology (Berlin) 16(1): 37-40. 

 No abstract. 

Carpenter, E., B.B. Peck and S.J. Anderson. 1974a. Copepod and chlorophyll: A 

concentrations in receiving waters of a nuclear power station and problems associated 

with their measurements. Estuarine Coastal Marine Science 2:83-88. 

 No abstract. 

Carpenter, E., B.B. Peck and S.J. Anderson. 1974b. Summary of entrainment research at 

the Millstone Point Nuclear Power Station, 1970 to 1972. Pp. 31-35. In: L.D. Jensen 

(ed). Proceedings of the Second Workshop on Entrainment and Intake Screening. The 

Johns Hopkins University, Department of Geography and Environmental Engineering, 

Baltimore, MD. 

 No abstract. 

Carpenter, E., B.B. Peck and S.J. Anderson. 1974c. Survival of copepods passing 

through a nuclear power station on Long Island Sound, USA. Marine Biology 24: 49-55. 

25 
 About 70% of the copepods entering the cooling water system of a nuclear power 

plant on northeastern Long Island Sound (USA) are not returned to the Sound in the 

effluent. Copepod mortalities are caused by the mechanical or hydraulic stresses of 

passage, although our experimental design could not determine whether heat or 

chlorination could cause mortality in the absence of mortality induced by hydraulic 

stress. After passing through the power plant, copepods sink rapidly (~2.5 times faster 

than controls). This leads to an increase in concentrations of copepods suspended in the 

deep water (25 to 30 m) of the effluent pond. About half of the live copepods collected at 

the discharge and held in situ died within 3.5 days, and 70% died within 5 days, whereas 

only 10% of those from the intake died in 5 days. About 60% of the copepods observed 

suspended in deep water in the pond were dead. The copepod mortality caused by the 

power plant reflects the loss in secondary production occurring below about 270×103

 m

2

of sea surface in Long Island Sound annually. This loss represents a reduction of about 

0.1% in the annual secondary production over a 333 km2

 area of Long Island Sound 

adjacent to the power plant. Highest losses occurred during the spring (April, 1.4×106

 g 

dry weight), the lowest in autumn (November, 45.8×103

 g). If the same copepod loss rate 

exists for all power plants in Long Island Sound, then secondary production in 1.69×106

m

2

, or 0.05% of the total copepod production may be lost annually. A comparison of the 

surface outflow from Long Island to Block Island Sound with the water entrained through 

Millstone Unit One, and the 70% copepod loss rate in the latter area, indicates that Unit 

One eliminates about 0.1 to 0.3% of the copepod production in eastern Long Island 

Sound. This calculation compares favorably with losses computed from production data. 

 

Carpenter, J.H., C.A. Smith and R.G. Zika. 1981. Reaction products from the 

chlorination of seawater. Final report 15 Jul 75-14 Jul 80 PB-81-172280. Miami, FL 

(USA), Miami Univ., FL Rosenstiel School of Marine and Atmospheric Sciences, 62 pp. 

 Chemical treatment of natural waters, in particular the use of chlorine as a 

biocide, modifies the chemistry of these waters in ways that are not fully understood. 

The research described in this report examined both inorganic and organic reaction 

products from the chlorination of seawater using a variety of analytical approaches. 

Some analytical methods in widespread current use underestimate the residual oxidants in 

chlorinated seawater by as much as 70% depending upon the detail of the procedure. The 

chlorination of seawater in the presence of light produces substantial quantities of 

bromate ions which can influence standard analytical procedures and represents an 

unknown factor in estuarine and coastal waters. The copper complexing capacity of 

Biscayne Bay, Florida water was found to be substantially reduced with the addition of 

chlorine. Analysis was made by anodic stripping voltammetry on water samples after 

successive additions of copper sulfate solution. Laboratory chlorination of water from 

the intake of the Port Everglades, Florida power plant produces bromoform levels 

comparable to that found in the plant discharge. These results are in contrast to results 

reported in the literature for a power plant on the Patuxent estuary in Maryland, so that 

bromoform production appears to be site-specific. Chloroform extracts of chlorinated 

Biscayne Bay water are found to contain halogenated compounds which are new and 

different, and which pose unusual analytical problems. Studies using GC/ECD, GC/MS, 

26 
HPLC, H NMR, differential pulsed polarography and other techniques on natural extracts 

and synthesized compounds are reported. 

Chang, W.Y.B. and R. Rossmann. 1984. Effects power plant entrainment on 

phytoplankton response, International Association of Theoretical and Applied 

Limnology/Travaux Conference. Congress of the International Association of 

Limnology- Lyon, France. 21 Aug 1983. 

 Effects of power plant entrainment on phytoplankton response consist primarily 

of four types: thermal and hydromechanical impacts, and the impacts of biofouling 

control and of erosion and corrosion of power plant components. A study focusing on 

these issues was conducted between February 1980 and May 1982 at the D.C. Cook 

Plant on the southeastern shore of Lake Michigan. The results show that changes in 

chlorophyll and phaeophytin concentration are not significant in water which has passed 

through the condenser cooling system, but the reduction in primary productivity as 

measured by C14 was remarkable, ranging from 20% similar to 80%. While the former 

show no significant damage done by increased temperature and hydromechanical 

abrasion on phytoplankton, the latter indicates a significant inhibition of the 

photosynthesis of these phytoplankton. 

Chapman, G.A., D.L. Denton and J.M. Lazorchak. 1995. Short-term methods for 

estimating the chronic toxicity of effluents and receiving waters to west coast marine and 

estuarine organisms U.S. EPA, National Exposure Research Laboratory, Office of 

Research and Development. 661 pp. 

 No abstract. 

 

Choi, D.H., J.S. Park, C.Y. Hwang, S.H. Huh and B.C. Cho. 2002. Effects of thermal 

effluents from a power station on bacteria and heterotrophic nanoflagellates in coastal 

waters. Marine Ecology Progress Series 229: 1-10. 

 To investigate effects of thermal effluents from a coastal power station on bacteria 

and heterotrophic nanoflagellates (HNF) in adjacent coastal waters, surface distributions 

of bacteria and HNF and interrelations between microbial and environmental variables 

were studied in November 1998 and July, August and November 1999 near Hadong 

Power Station, Korea. In addition, manipulation experiments with treatments of high 

temperature (40°C; temperature in the cooling system of the power station) and additions 

of hypochlorite and dilution experiments of thermal effluents with intake seawaters were 

carried out. Water temperature in the discharge-channel was always 5 to 10°C higher 

than the ambient temperature, but bacterial production, HNF abundance and grazing rates 

on bacteria, as well as chlorophyll a concentrations, were always lower there and 

increased with distance from the power station. Manipulation experiments showed that 

addition of hypochlorite had much more deleterious effects on bacteria and HNF (95 to 

98% inhibition of bacterial production and 25 to 45% decrease in HNF abundance at 0.13 

ppm of residual chlorine) than high temperature (9 to 39% inhibition of bacterial 

production but no inhibition of HNF abundance at 40°C). Dilutions of thermal effluents 

27 
from the outlet and condenser tube with intake seawater would bring < 0.03 ppm of 

residual chlorine, but inhibitory effects of thermal effluents on bacterial production and 

HNF grazing activity (23 to 69% inhibition of bacterial production and 31 to 36% 

inhibition of HNF grazing) were shown, indicating inhibitory potential of chlorination 

by-products in the discharged water on coastal microbes. Analyses of horizontal 

distributions of microbial variables, manipulation and dilution experiments gave 

consistently similar results of inhibitory effects of thermal effluents on bacteria and HNF. 

Our work suggests that a microbial ecological approach is useful in estimating influences 

of thermal Pollution on microbes in aquatic environments. 

Cooke, S.J. and J.F. Schreer. 2001. Additive effects of chlorinated biocides and water 

temperature on fish in thermal effluents with emphasis on the Great Lakes [of North 

America]. Reviews in Fisheries Science 9(2): 69-113. 

 We reviewed the literature on the effects of chlorine on selected Great Lakes 

fishes during the summer when chlorine is used to control biofouling in cooling systems 

at power generating stations. Mortalities of fish are usually not solely due to chlorine 

toxicity but to complex additive functions and interactions of various stressors, in 

particular temperature. Elevated temperature appears to be important in magnifying the 

effects of the toxicity of chlorine to fish. When chlorination is used at temperatures near 

the thermal maxima, but not sufficiently high to exclude fish, high mortality rates can be 

expected. Most of the fish that lose equilibrium during exposure do not survive. Fish 

exposed to sublethal levels of chlorine become lethargic and often gulp air and frequently 

suffer increased predation pressures from birds and other fish. Additionally, 

hematological and biochemical disturbances, and potentially irreversible gill damage, 

may impair the lifetime fitness of fish exposed to chlorine. The sensitivity of different 

species of fish to chlorine toxicity varies widely. As such, chlorination regimes should be 

evaluated on a daily basis to account for differences in species composition and water 

temperature Most of the chlorine exposure concentrations reported in the literature are for 

50% mortality, but the highest concentration resulting in no mortality, loss of 

equilibrium, or sublethal effects, is a more appropriate value for management and 

conservation. We also advocate comprehensive ecological risk assessments to determine 

the scope of impact on all organisms, not just fish. Only a series of in situ and laboratory 

studies for each situation will provide biologically meaningful values and the basis for 

relevant regulations. 

Cripe, C.R. 1979. An automated device (AGARS) for studying avoidance of Pollutant 

gradients by aquatic organisms. Journal Fisheries Research Board Canada 36: 11-16. 

 No abstract. 

Davies, R.M. and L.D. Jensen. 1975. Zooplankton entrainment at three mid-Atlantic 

power plants. Journal Water Pollution Control Federation 47: 2130-2142. 

 No abstract. 

28 
Davis, M.H. and J. Coughlin. 1978. Response of entrained plankton to low-level 

chlorination at a coastal power station. Pp. 369-376. In: Jolley, R.L., H. Gorchev and 

D.H. Hamilton (eds). Water Chlorination Environmental Impact and Health Effects Vol. 

2 Ann Arbor Science Publishers, Inc., Ann Arbor, MI. 

 No abstract. 

Dempsey, C.H. 1986. The exposure of herring postlarvae to chlorine in coastal power 

stations. Marine Environmental Research 20 (4): 279-290. 

 Postlarvae of Clupea harengus were exposed to chlorinated sea water for 30 

minutes, to simulate passage through a typical power station cooling water circuit, and 

24-h, during which detectable chlorine decayed away, to simulate a 'worst case' exposure. 

Twenty-four hour LC50s were 0.63ppm initial concentration for 30 minutes exposure and 

0.36ppm initial concentration for 24-h exposure. 

Domart-Coulon, I., S. Auzoux-Bordenave, D. Doumenc and M. Khalanski. 2000. 

Cytotoxicity assessment of antibiofouling compounds and by-products in marine bivalve 

cell culture Toxicology In Vitro 14(3): 245-251. 

 Short-term primary cell cultures were derived from adult marine bivalve tissues: 

the heart of oyster Crassostrea gigas and the gill of clam Ruditapes decussatus. These 

cultures were used as experimental in vitro models to assess the acute cytotoxicity of an 

organic molluide, Mexel-432 registered, used in antibiofouling treatments in industrial 

cooling water systems. A microplate cell viability assay, based on the enzymatic 

reduction of tetrazolium dye (MTT) in living bivalve cells, was adapted to test the 

cytotoxicity of this compound: in both in vitro models, toxicity thresholds of Mexel-432 

were compared to those determined in vivo with classic acute toxicity tests. The clam 

gill cell model was also used to assess the cytotoxicity of by-products of chlorination, a 

major strategy of biofouling control in the marine environment. The applications and 

limits of these new in vitro models for monitoring aquatic Pollutants were discussed, in 

reference with the standardized Microtox registered test. 

Dressel, D.M. 1971. The effects of thermal shock and chlorine one the estuarine 

copepod, Acartia tonsa. MS thesis, University of Virginia, Charlottesville, VA. 

 No abstract. 

Enstrom, D.G and J.B. Kirkwood. 1974. Median tolerance limits of selected marine fish 

and lobster larvae to temperature and chlorinity. Appendices IIB3 in Supplement to 

Preoperational Report on Pilgrim Nuclear Studies. Boston Edison Co., Boston, MA. 

 No abstract. 

29 
Eppley, R.W., E.H Renger and P.M. Williams. 1976. Chlorine reactions with seawater 

constituents and the inhibition of photosynthesis on natural marine phytoplankton. 

Estuarine Coastal Marine Science 4: 147-161. 

 The time course of chlorine disappearance was followed in filtered (1), nonfiltered (2), and u.v.-oxidized (3) seawater. In (1) and (2) there was an initial rapid 

decline in both free and residual chlorine followed by a slower decline of these species. 

No decline was observed in (3), suggesting that the removal of organic matter and/or the 

oxidation of metal ions by u.v.-oxidation removed those molecular species that reacted 

with chlorine. Reaction rate was more rapid in (2) than in (1), suggesting that particulate 

organic matter may also react with chlorine. Reaction kinetics of chlorine were complex 

but approximated second order in the slow phase. The inhibition of phytoplankton 

photosynthesis by chlorine was studied by adding hypochlorite to samples. Chlorine 

concentrations required for 50 % inhibition varied with exposure time. For 24-h 

incubations such inhibitions took place with residual chlorine concentrations of 109

. The 

inhibition appeared to be irreversible. The deleterious impact on marine phytoplankton 

by the use of seawater for cooling power plants lay in the use of chlorine to prevent slime 

build-up on the condenser system parts. 

Erickson, S.J. and A.J. Freeman. 1978. Toxicity screening of fifteen chlorinated and 

brominated compounds using four species of marine phytoplankton. Pp. 307-310. In: 

Jolley, R.L., H. Gorchev and D.H. Hamilton (eds). Water Chlorination Environmental 

Impact and Health Effects Vol. 2 Ann Arbor Science Publishers, Inc., Ann Arbor, MI. 

 No abstract. 

Erickson, S.J. and H.R. Foulk. 1980. Effects of continuous chlorination on entrained 

estuarine plankton. Journal Water Pollution Control Federation 52(1): 44-47. 

 The effects of continuous chlorination in running sea water on entrained plankton 

were examined. The concentration of ATP was used as an indicator of biomass because: 

it is present in all living cells; the concentration is proportional to the living biomass; and 

dead cells lose ATP rapidly. Effects were measured by biominescence; luciferinluciferase reagents from firefly lanterns were used to analyze ATP concentration. Results 

indicate that ATP measurement is an accurate, effective means of evaluating damage 

done to planktonic organisms by continuous chlorination. Further studies of the effects 

of low-concentration, continuous chlorination are recommended. 

Fayad, N.M. and S. Iqbal. 1987. Chlorination byproducts of Arabian Gulf seawater. 

Bulletin of Environmental Contamination and Toxicology 38(3): 475-482. 

 Some researchers have related the toxicity of chlorinated seawater to the residual 

chlorine contents of the water. This has resulted in the establishment of guidelines for 

reducing the chlorine levels in the discharged water. However, when chlorine is added to 

seawater, a great proportion of it is consumed in the oxidation of the naturally occurring 

organic and inorganic materials of the water. Therefore, the harmful effects of the 

30 
chlorinated discharges can most probably be attributed to the reaction by-products. Very 

little information is available about these by-products. 

Ferro-Soto, B. 2001. Effect of chlorine on different bacterial groups and effectiveness of 

the purification of sewage in harvested areas. Journal of Shellfish Research 20(3): 1311-

1314. 

 Besides microbiological monitoring programs, it is important in shellfish 

harvesting areas to understand the process of purification of sewage discharged in 

harvesting areas. The objective of this study was to evaluate the importance of 

purification, the effectiveness of chlorination, and the influence in these areas. Chlorine 

produced significant reductions on all studied bacterial groups and reductions were 

among one and two orders of magnitude, being lower for fecal streptococci and aerobic 

bacteria at 25°C. High quantitative variability was observed with regard to the number of 

bacteria presents in these waters (W1, W2, W3) but qualitative variability is unknown. 

Correlation among shellfish samples and W3 (effluent discharged in harvesting areas) 

was not observed. 

Flemer, D.A. 1974. The effects of entrainment on phytoplankton at the Morgantown 

Steam Electric Station Potomac River Estuary, September 5-8, 1972. Pp. 163-164. In: 

L.D Jensen (ed). Proceedings of the Second Workshop on Entrainment and Intake 

Screening. The Johns Hopkins University, Department of Geography and Environmental 

Engineering, Baltimore, MD. 

 No abstract. 

Flemer, D. and J.A. Sherk. 1977. The effects of steam electric station operation on 

entrained phytoplankton. Hydrobiologia 55:33-44. 

 The effect of entrainment on estuarine phytoplankton was studied at 3 steam 

electric stations located on tributaries of Chesapeake Bay [USA]. In most cases, Cl rather 

than heat was implicated as the most important factor in the reduction of the rate of C 

assimilation. The reduction of C assimilation in the mixture of plant effluent and 

augmentation water was greater than explained as mechanical and heat effects. Cl was 

judged as the primary factor under these conditions. One study that involved 3 

consecutive days exemplified the problem of interpreting significant statistical 

interactions and main effects in a multivariate design when tests were conducted under 

natural conditions. 

Fox, J.L. and M.S. Moyer. 1975. Effect of power plant chlorination on estuarine 

productivity. Chesapeake Science 16(1): 66-68. 

 Power plants chlorinate their cooling water to prevent biological fouling of 

condenser tube walls. The authors studied the effects of chlorine on in site net primary 

production in the discharge waters of a power plant at Crystal River, Florida. Primary 

31 
production values decreased an average of 57% due to plant passage and chlorination. In 

the absence of chlorine, the average decrease was 13%. 

 

Galya, D., M. Scherer, J. Herberich, S. Kelly and J. Scheffer. 2004. Assessment of 

power plant entrainment in comparison to long-shore ichthyoplankton transport. Flatfish 

Biology Conference 2004, Westbrook, CT (USA), 1-2 Dec 2004. 

 No abstract. 

Galtsoff, P.S. 1946. Reaction of oysters to chlorination. Report No. 11, U.S. 

Department of the Interior, U.S. Fish and Wildlife Service, Washington, DC. 

 No abstract. 

 

Gaudy, R. and B. Moatti. 1978. Study of the mortality of zooplankton transient in the 

cooling waters of a thermoelectric power plant. Symposium on Pollution of the 

Mediterranean, Antalya, Turkey. 

 The mortality of zooplankton sampled from intake and effluent circuits of a power 

plant (Martigues-Ponteau, Gulf of Fos, France) has been followed during a 18 months 

study. A vital staining technique was used to determine the proportion of dead and live 

animals under different conditions of power plant activity. The sampling process, added 

to the natural mortality caused a mortality rate of 25%. The lethal factors introduced by 

the cooling water system were: turbulence (mechanical shock in the circuits) 21%; 

thermal shock, whose effect is correlated with the increase of t (about 0 to 12%) and 

chlorination (31% average). The mortality appeared to affect larger and smaller forms of 

zooplankton equally. These results are discussed and compared with previous 

estimations. 

Gavaskar, A.R. and A.S.C. Chen. 2002. Proceedings of the third international 

conference on remediation of chlorinated and recalcitrant compounds. Monterey, CA, 

May 2002. ISBN: 1-57477-132-9. Compact disc available for $295 from Battelle, 505 

King Ave., Columbus, OH 43201-2693. 

 The Third International Conference on Remediation of Chlorinated and 

Recalcitrant Compounds (May 2002) was attended by approximately 1,450 scientists, 

engineers, regulators, remediation site owners, and other environmental professionals 

representing universities, government site management and regulatory agencies, and 

R&D and manufacturing firms from 36 countries. 

The 320 papers in this proceedings address the challenge of characterizing, remediating, 

monitoring, and closing sites contaminated with chlorinated solvents and other 

recalcitrant compounds. At sites with these contaminants, many factors can challenge the 

technical effectiveness and economics of the remedial action. Papers in the Remediation 

Project Planning and Site Characterization section discuss regulatory interaction (e.g., 

Risk-Based Corrective Action [RBCA]) and describe approaches for design and 

modeling of remediation projects, selection and use of data management tools, and site 

32 
characterization methods for a wide range of media and contaminants, determination of 

achievable cleanup goals, and remedy selection/design. The Remediation Technologies 

section contains information on selecting from the spectrum of remediation technologiesphysical, biological, chemical, thermal, natural attenuation, and combined-and methods 

of delivering the chosen treatment technology to the contaminants effectively and 

economically and ways to monitor the progress of the remediation. Post-Remediation 

Strategies presents ways to accomplish the monitoring necessary to determine the 

achievement of short-term performance endpoints that meet regulatory and economic 

goals. Also addressed are design and implementation of short-term exit strategies and 

long-term monitoring programs. 

Gavaskar, A.R. and A.S.C. Chen. 2005. Proceedings of the fourth international 

conference on remediation of chlorinated and recalcitrant compounds. Monterey, CA, 

May 2004. ISBN: 1-57477-145-0. Compact disc available for $295 from Battelle, 505 

King Ave., Columbus, OH 43201-2693. 

 The Fourth International Conference on Remediation of Chlorinated and 

Recalcitrant Compounds (May 2004) was attended by approximately 1,425 scientists, 

engineers, regulators, remediation site owners, and other environmental professionals 

representing universities, government site management and regulatory agencies, and 

R&D and manufacturing firms from 26 countries. 

This proceeding consists of 682 documents, representing essentially all platform talks and 

poster presentations made at the Conference. The 322 papers and 360 abstracts address 

the challenge of characterizing, remediating, monitoring, and closing sites contaminated 

with chlorinated solvents and other recalcitrant compounds. Among the topics covered 

are chlorinated solvent plumes, DNAPL, arsenic, mercury, MTBE, perchlorate, 

energetics, PCBs, and PAHs in groundwater, soil, fractured bedrock, and sediments. 

New developments in site characterization and remediation technologies, such as 

permeable barriers, chemical oxidation, thermal treatment, biological remedies, and 

natural attenuation are addressed. 

The papers and abstracts are organized into the following sections: 

Site Assessment and Characterization 

DNAPL Source Remediation Technologies 

Solvent Plume Control and Treatment Technologies 

Remediation of Other Recalcitrant Compounds and Matrices 

Managing the Remediation and Post-Remediation Process. 

Gentile, J.H., J. Cardin, M. Johnson and S. Sosnowski. 1976. Power plants, chlorine, 

and estuaries Ecol. Research Series U.S. Environmental Protection Agency 

76-055: 37. 

 Biological assay systems using indigenous holo- and meroplankton were designed 

to model the chlorination patterns of power plants. A matrix of chlorine concns and 

exposure patterns permitted the generation of response isopleths that were then applied to 

developing design criteria. The marine phytoplankter, Thalassiosira pseudonana showed 

33 
a 50% reduction in photosynthesis when exposed to 0.15 ppm Cl2 for 10 minutes, and 

complete growth inhibition after 5 minutes exposure to 0.3 ppm. Microzooplankton 

adults were somewhat less sensitive in that a 5 minute exposure at 2.5 ppm was necessary 

to produce 50% mortality. Larval and juvenile fish were sensitive to chlorine levels ≤ 0.2 

ppm for exposure periods of 60 to 90 minutes. 2 field studies were evaluated and 

compared to lab data with specific emphasis on the use of ATP to monitor entrainment 

and damages. A review of pertinent literature is also included. 

Gibson, C., F. Tone, P. Wilkinson, J. Blaylock and R. Schirmer. 1981. Toxicity, 

bioaccumulation and depuration of bromoform in five marine species: Protothaca 

stamineae', (littleneck clam), Mercenaria mercenaria, (eastern hard clam), quahog, 

Crassostrea virginica, (eastern oyster), Penaeus aztecus, (brown shrimp), Brevoortia 

tyrannus, (Atlantic menhaden). Government Reports Announcements. Battelle, 

Richland, WA, Pacific Northwest Lab Report. 53 pp. 

 Bromoform has been identified as the single most abundant halogenated 

organic compound produced by the chlorination of marine waters. To determine the 

potential Biological effects of its release into marine waters, short-term toxicity bioassay 

and 28-day uptake/28-day depuration studies were conducted with five marine species: 

Protothaca staminea, Mercenaria mercenaria, Crassostrea virginica, Penaeus aztecus, 

and Brevoortia tyrannus. These species have commercial and economic importance and 

are often found in the vicinity of discharge streams from nuclear fueled steam electric 

stations. 

Gibson, C.I., T.O. Thatcher and C.W. Apts. 1976. Some effects of temperature, 

chlorine, and copper on the survival and growth of the coon stripe shrimp. Thermal 

Ecology Vol 2: 88-92. 

 Bioassay and growth-rate experiments were conducted on coon stripe shrimp to 

determine the LL50 value for heat and the LC50 values for chlorine and copper, and the 

effect of sublethal concentrations of these materials on the growth rate of the shrimp. 

Critical Thermal Maximum (CTM) values were found to increase with an increase in size 

and rate at which the temperature was elevated. Shrimp were more resistant to chlorine 

when acclimated and exposed at 8-10°C than when acclimated at 8°C and exposed at 15 

or 20°C or when acclimated and exposed at 15°C. Optimal growing temperature for 

periods up to one month was 16°C. Copper concentrations of 0.041 mg/L effectively 

retarded the growth at 16°C over a 1-month period. Chlorine concentrations of 0.18 

mg/L were lethal at 16°C and reduced growth of the shrimp at 0.08 mg/L over a 1-month 

period. (See also W77-05541) 

"Gift's Bioassy Work". 1973. Appendices Vol. IV, Sect. IIIA in Environmental Report, 

Indian Point No. 3. Consolidated Edison Co. of New York, Inc., New York, NY. 

 No abstract. 

34 
Ginn, T.C. and J.M. O' Connor. 1978. Response of the estuarine amphipod Gammarus 

daiberi to chlorinated power plant effluent. Estuarine Coastal and Shelf Science 6(5): 

459-469. 

 The amphipod G. daiberi was subjected to plume drifts and laboratory exposures 

to chlorinated power plant cooling water effluent. Behavioral reactions to unchlorinated 

and chlorinated effluent were examined in an avoidance trough. During plant 

chlorination, the amphipods survived 1-h exposures to full-strength cooling water 

effluent (ΔT = 7.3-9.3°C, 0.05 ml-1 total chlorine). Test groups of G. daiberi drifted 

through the discharge plume during chlorination displayed no increased immediate or 

latent mortalities. One-hour static bioassays revealed an initial TL50 value of 1.85 mg/L 

total chlorine, which decayed to 0.2-0.5 mg/L during the exposure period. At ambient 

temperatures of 26.4-26.6°C, the amphipods avoided unchlorinated effluent at 

ΔT's>3.3°C. At lower ambient temperatures (15.3-15.7°C), no avoidance was observed 

at a 7.1°C ΔT. Chlorinated discharge water was avoided by G. daiberi at both ambient 

temperatures. 

Ginn, T.C., W.T. Waller and G.J. Lauer. 1974. The effects of power plant condenser 

cooling water entrainment on the amphipod, Gammarus sp. Water Research 8: 937-945. 

 The abundant Hudson River amphipod Gammarus sp. was examined for viability 

before and during entrainment in the Indian Point cooling water system. The mean 

percent survivial of Gammarus sp. sampled during ΔT's of 7.1–8.3°C and ambient 

temperatures of 24.9–26.0°C was 98.5 and 97.4% for the two intake stations and 90.1 and 

96.8% for the discharge canal stations D-1 and D-2 respectively. A statistically 

significant (α = 0.05) difference was detected between the survival of Gammarus sp. at 

the intake stations and discharge station D-1, located near the upper end of the discharge 

canal. Entrained Gammarus sp. experience increased initial and latent mortalities during 

periods of condenser chlorination. Comparison of the abundances of entrained 

Gammarus sp. during day and night sampling periods reveals a significantly higher 

occurrence during darkness. Temperature bioassays indicate that the thermal tolerance of 

Gammarus sp. is dependent on exposure time and ambient temperature. The temperature 

resulting in a 50% mortality of Gammarus sp. for 30 minutes exposure times increased 

approximately 11°C as ambient temperatures increased from 2.5 to 25.8°C. 

Consequently, Gammarus sp. was capable of surviving ΔT's of greater magnitude as the 

ambient temperature to which they were acclimated decreased. Temperature bioassays 

indicated that Gammarus sp. should be able to tolerate all projected time-temperature 

exposure combinations encountered during entrainment through the cooling water 

system. 

Goldman, J.C. 1978. Combined toxicity effects of chlorine, ammonia, and temperature 

on marine plankton. Progress report, November 1976-31 January 1978. COO-2532-3 

DOE Contract Number EY-76-S-02-2532 W.H.O. Institution. Woods Hole, MA (USA), 

Woods Hole Oceanographic Institution Report. 6 pp. 

 Studies on the effects of chlorine, chloramines, and temperature on marine 

plankton have been carried out for three years. Species studied include marine 

35 
phytoplankton, lobster larvae (Homarus americanus), oyster larvae (Crassostrea 

virginica), copepods (Acartia tonsa), rotifers (Brachionas plicatilis), grass shrimp 

(Palamonetes pugio) summer flounder larvae (Paralichthys dentatus), larval and juvenile 

killifish (Fundulus heteroclitus), juvenile scup (Stenotomus versicolor), and juvenile 

winter flounder (Pseudopleuronectes americanus). In addition extensive studies on 

chlorine chemistry in seawater have been carried out. The major conclusions are that 

entrainment effects on permanent plankton such as phytoplankton, copepods, and rotifers 

are temporary, that is those organisms surviving chlorination and temperature shocks are 

capable of renewed and unrestricted growth once returned to the receiving water. 

Because chlorine is only applied for short periods daily in most power plants, the total 

population of the above organisms actually exposed to chlorine is small and the effects 

may be hardly measurable in receiving waters. However, chlorination effects on larval 

species that spawn intermittently could be catastrophic. In addition, there are many 

unanswered questions regarding the fate of chlorine that is dissipated in marine waters. 

Are the losses real and, if so, do they pose a toxicity threat to marine biota. 

Goldman, J.C. and J.A. Davidson. 1977. Physical model of phytoplankton mortality at 

coastal power plants. Environmental Science and Technology 11(9): 908-913. 

 A physical model of the impact of coastal power plant chlorination on marine 

phytoplankton was developed. Several steady state biomass levels of the test alga 

Phaeodactylum tricornutum were established in continuous cultures by varying the 

concentration of P in the growth medium. The algae were then exposed to combinations 

of chlorine and temperature stress for 1 h, and deviations from the prestress steady state 

were observed over long periods of time. Three types of response occurred that were a 

function of the applied chlorine/biomass ratio: no effect, effect followed by recovery, and 

complete washout. It is suggested from the results that, given the typical organic loads 

present in coastal waters, excessive levels of chlorine may be in current use, and that the 

effects on entrained phytoplankton probably have little bearing on the ultimate effect on 

the standing crop in the receiving water. The most important considerations were the 

hydrologic, Biological and chemical qualities of the receiving waters themselves. 

Goldman, J.C. and H. Quinby. 1979. Phytoplankton recovery after power plant 

entrainment. Journal Water Pollution Control Federation 51(7): 200-206. 

 Natural populations of marine phytoplankton from the intake and discharge 

stations of 2 coastal power plants in southeastern Massachusetts [USA] all demonstrated 

the same degree of recovery when grown in continuous cultures with a defined artificial 

seawater medium. Neither populations exposed to elevated temperatures nor those 

subject to chlorination and heat treatment showed any adverse permanent effects. The 

flora of phytoplankton species dominating in samples for a particular experiment were 

consistently similar, indicating that alterations in the composition of phytoplankton 

species in receiving waters may be hardly measurable. Entrainment effects on both 

phyto- and permanent zooplankton populations in receiving waters, because of their 

relatively rapid generation periods, are probably minor compared with the potentially 

major effects on larval plankton that spawn intermittently. 

36 
Goldman, J.C., J.M. Capuzzo and G.T. Wong. 1978. Biological and chemical effects of 

chlorination at coastal power plants. Pp. 291-305. In: Jolley, R.L., H. Gorchev and D.H. 

Hamilton (eds). Water Chlorination Environmental Impact and Health Effects. Vol. 2 

Ann Arbor Science Publishers, Inc., Ann Arbor, MI. 

 No abstract. 

Graslund, S., P.A. Bergqvist and B.E. Bengtsson. 1999. Potential formation of toxic byproducts from the use of chlorine as a disinfectant in intensive shrimp farming in 

Southeast Asia. Fourth Symposium on Diseases in Asian Aquaculture: Aquatic Animal 

Health for Sustainability. Cebu International Convention Center, Waterfront Cebu City 

Hotel, Cebu City, Philippines, Asian Fisheries Society, Manila (Philippines) Fish Health 

Section. 12 pp. 

 The purpose of this study was to elucidate whether halogenated hydrocarbons are 

formed as a side effect following chlorination of shrimp ponds, and to see if these byproducts might cause toxicity to biota. Disinfection of shrimp ponds by application of 

chlorine is widely recommended as a disease preventing measure in intensive shrimp 

farming in South East Asia. In Thailand alone there is an estimated 50,000 tons of 

chlorine annually used in shrimp farms. The fate of chlorinated substances in shrimp 

ponds is not known until now. On the contrary chlorination of drinking water has been 

subject to many studies. These generally show that chlorine added to natural waters react 

with organic substances and result in significant concentrations of halogenated 

hydrocarbons. Several of these substances are known to be toxic to aquatic organisms or 

humans. An experiment was conducted in experimental ponds set up as a controlled 

model of shrimp ponds, i.e., filled with sediment and salt water. The ponds were treated 

with calcium hypochlorite at doses ranging from normal dose to 150 times the normal 

rate. Black tiger shrimp, Penaeus monodon, were put in each pond 4 days after 

chlorination. Sampling for analyzes was made from water, air, sediment and shrimp. 

Passive sampling with semi-permeable membrane devices (SPMDs) was made in the 

pond water and in the air close to the water surface during a period of 12 days with start 

just before chlorination. Simultaneously, passive sampling was terminated and shrimp 

and sediment were collected. SPMD samples were prepared for analysis by dialysis. The 

lipophilic phase was extracted from shrimp hepatopancreas and sediment. Each sample 

was run through a GPC in a HPLC in order to fractionate the sample content. The 

fraction of the size of diethylhexylphtalate and smaller was collected. This fraction was 

run in a GC/ECD in order to detect halogenated hydrocarbons. Part of the same fraction 

was tested for toxicity in Microtox assays. Results of the substances contained in the 

samples and their toxicity in Microtox assays will be presented at the symposium. 

Whether there is a correlation between occurrence of halogenated organic compounds 

and toxicity seen in Microtox assays will be answered, as well as the question of how the 

formed substances are distributed in different compartments of the system - water, 

sediment, air and shrimp. 

37 
Gullens, S.R., R.M. Block, J.C. Rhoderick, D.T. Burton and L.H. Liden. 1977. Effects 

of continuous chlorination on white perch (Morone americana) and Atlantic menhaden 

(Brevoortia tyrannus) at two temperatures. Research Association Southeastern Biologists 

Bulletin 24: 55 pp. 

 No abstract. 

Haag, W.R. 1981. Chemistry of saline-water chlorination. Oak Ridge National Lab and 

Tennessee Univ., Knoxville, TN (USA). 160 pp. 

 Vast quantities of natural waters are used by power plants for cooling purposes. 

This water is chlorinated to prevent slime build-up inside the cooling pipes, is circulated 

through the cooling system, and eventually discharged back into the water body. In order 

to assess the environmental impact of water chlorination, it is necessary to know what 

chemical compounds are produced and discharged into the receiving waters. To attack 

this problem, a review of the present state of knowledge of natural water chlorination 

chemistry was performed, and some experimental work explained the results of previous 

workers by showing that chlorine losses at very high doses in seawater are simply the 

result of chlorate and bromate formation which, however, is negligible at normal doses. 

The most important chlorine-produced oxidants, along with the relevant chemical 

reactions, were chosen as a basis for a kinetic model of saline water chlorination 

chemistry. Kinetic data were compiled in a computer program which simultaneously 

solves 24 differential equations, one for each species modeled. Estimates were made for 

the unknown rate constants. A purely predictive model was not possible due to the great 

variability in the organic demand; however, the model is applicable under a broad variety 

of conditions (except sunlight), and it provides a reasonably good description of a 

halamine chemistry under environmental conditions. 

Hall, L.W., Jr., D.T. Burton and S.L. Margrey. 1979a. Chlorine, temperature and 

exposure duration efects of power plant effluents on juvenile blue crabs Callinectes 

sapidus and grass shrimp, Palaemonetes pugio. Journal of Toxicology Environmental 

Health 5: 749-757. 

 Effects of interacting Cl, temperature and exposure conditions similar to those 

found in power plant effluents were assessed for blue crab C. sapidus and grass shrimp P. 

pugio. Test organisms were exposed to total residual Cl (TRC) concentrations of 0.00, 

0.15 and 0.30 mg/L in combination with temperatures 2, 6 and 10°C above ambient for 

0.08, 2.0 and 4.0 h. TRC concentrations decayed over 1-1.5 h to < 0.01 mg/L. 

Temperatures decayed over 4 h to 2°C above ambient. These conditions were used to 

simulate Cl and temperature conditions in power plant discharge canals and near-field 

receiving streams. Regression model techniques were used to establish interaction of Cl, 

temperature excess (ΔT) and exposure duration as factors causing death up to 36 h after 

exposure. Mortality increased for grass shrimp as Cl concentration, ΔT, and exposure 

length increased. A percentage mortality model for grass shrimp shows that response to 

Cl concentration alone and ΔT alone is proportional to the square root of Cl concentration 

and ΔT, the effect of exposure duration is linear, and no 2nd-order interactions occurred 

38 
among variables tested. No significantly mortality was found for blue crabs for any 

variable tested. 

Hall, L.W., Jr., D.T. Burton and S.L. Margrey. 1979b. The effects of chlorine, elevated 

temperature and exposure duration of power plant effluents on larval white perch, 

Morone americana. Water Resources Bulletin 15: 1365-1373. 

 No abstract. 

Hall, L.W., Jr., D.T. Burton and S.L. Margrey. 1979c. The influence of acclimation 

temperature on the interactions of chlorine, elevated temperature, and exposure duration 

for grass shrimp, Palaemonetes pugio. Transnational American Fish. Society 108: 626-

631. 

 The effect of 15 and 27°C acclimation temperatures on interactions of Cl, 

elevated temperature (ΔT), and exposure duration was assessed for the grass shrimp. 

Test organisms were exposed to total residual Cl (TRC) concentrations of 0.00, 0.15 and 

0.30 mg/L in combination with ΔT of 2, 6 and 10°C above acclimation temperature and 

exposure periods of 0.08, 2.0 and 4.0 h. Cl concentrations were decayed over a 1-1.5 h 

period to < 0.01 mg/L TRC and temperatures were decayed over 4 h to 2°C above 

acclimation. Mortality observations were conducted for 96 h. Most of the deaths 

occurred 36 h after exposure to the 3 test variables. A percent mortality model for grass 

shrimp acclimated to 27°C showed that the response to Cl concentration alone and ΔT 

alone was proportional to the square root of either variable; the exposure duration effect 

was linear; and no 2nd-order interactions occurred among the variables tested. The 

model for grass shrimp acclimated to 15°C showed that ΔT had no effect; the response to 

Cl concentration was the dominant factor; and there existed a 2-way linear interaction 

between Cl concentration and exposure duration. 

Hall, L.W., Jr., D.T. Burton and S. Margrey. 1981a. Acclimation temperature: an 

important factor in power plant chlorination studies with larval white perch, Morone 

americana. Journal of Toxicology and Environmental Health 7(6): 941-50. 

 Larval white perch were subjected to interacting power plant conditions of total 

residual chlorine (0.00-0.30 mg/L), elevated temperature (2, 6, and 10°C), and exposure 

time (0.08, 2.0, and 4.0 h) at acclimation temperatures of 15 and 23°C. Mortality 

observations were conducted up to 96 h after exposure. Acclimation temperature was a 

significant factor contributing to the effects of simulated power plant conditions during 

the low and high thermal conditions. Larvae tested at 23°C generally showed greater 

mortality at all treatment levels than those tested at 15°C. Mortality of test organisms 

during a 4-h exposure at the higher acclimation temperature was greater than 85% at 

elevated temperatures greater than 6°C regardless of other treatment factors. Mortalities 

as low as 30% were observed at the lower acclimation temperature. 

39 
Hall, L.W., Jr., D.T. Burton, S.L. Margrey and K.R. Dixon. 1981b. Time-related 

mortality responses of striped bass (Morone saxatilis) ichthyoplankton after exposure to 

simulated power plant chlorination conditions. Water Research 15(7): 903-910. 

 Time related mortality responses of striped bass eggs and prolarvae were 

evaluated after exposure to interacting total residual chlorine (TRC), ΔT and exposure 

time representing condenser entrainment and effluent discharge conditions. Mortality 

observations were conducted at unequal time intervals for 36-h after exposure to the test 

conditions. A non-linear least square regression model was separately fit to the 

cumulative mortality data for both eggs and prolarvae. Analysis of the immediate 

mortality response (death at time 0) of both eggs and prolarvae showed no significant 

effects of ΔT or interactions between ΔT and the other variables; however, only a few 

hours after exposure ΔT does have a significant effect on the mortality response. 

Hall, L.W., Jr, G. Helz and D.T. Burton. 1981c. Power-plant chlorination: A Biological 

and chemical assessment. REPRI EA-1750 (Res Proj. 1312-1) Electric Power Research 

Insitute, 3412 Hillview Ave., Palo Alto, CA 94303. Final Report, December 1981. 

Prepared by the Academy of Natural Sciences of Philadelphia, 252 pp. 

 An assessment is presented of the pertinent literature on chlorine chemistry and 

toxicity and a listing of research recommendations based on the review. Chlorine 

reaction rates and paths, chemical species, oxidant analyses, and organic products are 

discussed. Freshwater, estuarine, and marine toxicity methodologies are considered as 

well as specific tests on many organisms. Research recommendations include refinement 

of existing methodologies and development of new techniques. (ERA citation 

07:044733) 

Hall, L.W., Jr., D.T. Burton and L.H. Liden. 1982. Power plant chlorination effects on 

estuarine and marine organisms. Critical Reviews in Toxicology. 10(1): 27-48. 

 The objectives of this review were to collect, synthesize and interpret 

phytoplankton, zooplankton, macroinvertebrate and fish chlorine toxicity data related to 

biofouling control in estuarine and marine thermoelectric power plants, and to identify 

deficiencies in available information in order to recommend future research concerning 

the ecological effects of power plant cooling water chlorination. Chlorine toxicity data 

and subsequent chlorine species as reported by the various investigators are presented in 

this review. 

Hamilton, D.H.J., D.A. Flemer, C.W. Keefe and J.A. Mihursky. 1970. Power plants: 

effects of chlorination on estuarine primary production. Science (NY) 169(3941): 197-

198. 

 Steam electric stations may reduce primary production of cooling water by 91% 

as a result of chlorine applications for control of fouling organisms. Bacterial densities 

and concs of chlorophyll a are also reduced. Slight stimulation of production may occur 

in the absence of chlorination. Based on the available supply of 'new' water, a maximum 

loss of primary production of 6.6% was calculated for the tidal segment of the Patuxent 

River adjacent to a steam electric station. 

40 
Harwood, M., C. Blaise and P. Couture. 1989. Algal interactions with the genotoxic 

activity of selected chemicals and complex liquid samples. Aquatic Toxicology 14(3): 

263-276. 

 Ultra-acute 4-h algal interaction experiments on the genotoxic activity of three 

chemicals (MNNG, 4NQO and 2AA) and 9 complex liquid samples (4 organic chemical 

manufacturing effluents, 2 associated intake water supplies, 2 inorganic manufacturing 

effluents, and 1 pulp and paper effluent) were undertaken with Selenastrum 

capricornutum. Genotoxic activity was determined with the SOS Chromotest on each 

pre-exposure test solution and on each post-exposure supernatant and algal cell extract. 

Responses with the chemicals ranged from no activity change for MNNG to detoxication 

for 4NQO and to detoxication/uptake for 2AA. Exposure to experimental light and 

centrifugation were also shown to have a significant effect in reducing the activity of 

2AA. All but one of the complex liquid samples proved to be genotoxic and exposure 

responses were varied. As well as effects noted for the chemicals, increased genotoxic 

potency was observed in 3 samples, suggesting the presence of pro-mutagens capable of 

being activated by algal metabolism. Our results indicate that phytoplankton could play 

an important positive (detoxication) or negative (activation, uptake) role in its 

interactions with genotoxicants present in complex liquid wastes discharged to the 

aquatic environment. 

Heinle, D.R. 1973. Effects of the Chalk Point Power Plant on pumped and entrained 

copepods, August 12, 1971 and May 16, 1972, with notes on the Vienna Generating 

Station. NRI Ref No. 73-74. College Park, MD: University of Maryland, Natural 

Resources Institute. 

 The rate of C 14-uptake, an index of photosynthetic rate, and the concentration of 

chlorophyll a, a measure of standing crop, were measured to estimate the effects of 

entrainment on phytoplankton. The rate of carbon assimilation appeared to depend on the 

ambient water temperature and the temperature rise experienced by the entrained 

organisms. It was concluded that the reduction in carbon assimilation was greater than 

could be explained by the effects of mechanical and thermal stress and that chlorine was 

an important factor in the reduction. 

Heinle, D.R. 1976. Effects of passage through power plant cooling systems on estuarine 

copepods. Environmental Pollution. 11: 39-58. 

 The effects of chlorination, heated water, and entrainment on the copepods 

Scottolana canadensis, Eurytemora affinis and Acartia tonsa, at three power plant sites in 

Chesapeake Bay were measured by vital staining, a technique that allowed the 

examination of more individual organisms for viability or mortality. The Vienna plant 

(located at the Nanticoke River) with the greatest temperature rise, highest absolute 

temperatures, lowest salinities, and no chlorination, caused no detectable mortalities. At 

the Clark Point plant (located on the Patuxent River) there were slight mortalities with no 

chlorination and substantial mortalities occurred only during chlorination. At the 

41 
Morgantown site (located in Maryland below the Route 301 bridge across the Potomac 

River estuary) significant mortalities occurred only when chlorine was used. In addition 

to the slight mortalities caused by temperature alone and the clear effects of chlorine, the 

data suggested a difference in sensitivity of the three species. In order of increasing 

sensitivity they are: S. canadensis, E affinis and A. tonsa. The statistical problems 

associated with precise estimates of numbers of organisms passing through the 

Morgantown plant are presumed to be due to inadequate sampling efforts and the lack of 

prior knowledge of the copepod distribution. 

Heinle, D.R. and M.S. Beaven. 1977. Effects of chlorine on the copepod Acartia tonsa. 

Chesapeake Science 18: 140. 

 The authors found LC50's of 0.175, 0.062, 0.028 mg/L of chlorine produced 

oxidants for adult and immature copepods (combined) of A. tonsa at 15°C and salinities 

of 10.4 to 11.8 ppt. Preliminary results with nauplii of A. tonsa suggest lower LC50's than 

those for adults at equivalent exposure times. Comparison of all of the available 

published and unpublished results for adults of A. tonsa suggest that the relationship 

between log LC50 and log time to 50% mortality are linearly related in spite of 

considerable variation in methods used by various investigators. As near-field concns of 

chlorine of 0.001 to 0.01 mg/L have been estimated adjacent to a power plant on an 

estuary and as high as 2 mg/L near sewage treatment plants discharging into estuarine 

waters, it is suggested that lethal exposures to chlorine might commonly occur. 

Heinle, D.R., H.S. Millsaps, Jr. and C.V. Millsaps. 1974. Zooplankton studies 

Morgantown. Pp. 157-161. In: L.D. Jensen (ed). Proceedings of Second Workshop on 

Entrainment and Intake Screening. Department of Geography and Environmental 

Engineering, The Johns Hopkins University, Baltimore, MD 

 No abstract. 

Hergott, S. 1977. Chlorinated compounds in coastal power plant cooling waters. 

Chesapeake Science 18(1): 119-128. 

 Results are presented based on 4 months of a 12 month study funded by EPA of 

chlorination practices at 5 coastal power plants in California. Typical cooling water 

system layout is discussed with special emphasis on chlorination procedure Halogen 

residuals are measured at the condenser inlet and this measured residual is the criterion 

for determining the chlorine injection rate. Variations among the power plants occur as 

to frequency and duration of chlorination, dosage, analytical methods and equipment. 

Field studies at 3 of the 5 power plants are discussed. Cooling water is sampled during 

chlorination cycle at 2 locations simultaneously and halogen residuals (tree and/or total) 

measured at approximately 2 minute intervals. Halogen residuals are determined 

amperometrically using Fisher and Porter titrators (Model 17T1010). Water samples are 

brought back to the laboratory for determinations of ammonia-nitrogen, total nitrogen, 

soluble nitrogen, chloride, bromide, and suspended solids. The chlorine dose is 

calculated from the chlorine injection rate and the cooling water flow-rate. Decay studies 

42 
are performed at the outfall to determine the persistence of the halogen residual. Results 

are given as graphs showing the temporal variation of the halogen residual during the 

chlorination cycle at various sampling points in the system. Explanations of the observed 

decay patterns are suggested in relation to water quality, tidal influences, and/or 

chlorination practices. 

Hergott, S.J., D. Jenkins, and J.F. Thomas. 1978. Power plant cooling water chlorination 

in northern California. Journal of Water Pollution Control Federation 50(11): 2590-2601. 

 The nature, levels, and persistence of chlorinated compounds in the discharges of 

five power plants in northern California are determined. Chlorination frequency varies 

among the plants. All effluents produce receiving water oxidant residual levels predicted 

to be chronically toxic to marine organisms. Receiving water from two plants shows 

levels predicted to be chronically toxic to freshwater organisms. Acutely toxic levels 

exist in the receiving waters at one site. 

Hinchee, R.E. 1998. First international conference on remediation of chlorinated and 

recalcitrant compounds. Monterey, CA, May 1998. ISBN: 1-57477-062-4. Six vol. set 

available for $349.50 from Battelle, 505 King Ave., Columbus, OH 43201-2693. 

 Restoration of sites contaminated by chlorinated and other recalcitrant compounds 

is the subject of increasing attention worldwide. The 1998 International Conference on 

Remediation of Chlorinated and Recalcitrant Compounds (held in Monterey California, 

May 18-21, 1998) was the first in a series of biennial conferences focusing on the more 

problematic substances-chlorinated solvents, pesticides/herbicides, PCBs/dioxins, 

MTBE, DNAPLs, and explosive residues-in all media. Physical, chemical, biological, 

thermal, and combined technologies for dealing with these compounds were discussed, 

along with natural attenuation, site characterization, and monitoring technologies. Pilotand field-scale studies were presented, along with supporting laboratory data. Several 

sessions focused on human health and ecological risk assessment, regulatory issues, 

technology acceptance, and resource allocation and cost issues. 

The Conference was attended by scientists, engineers, managers, consultants, and 

other environmental professionals representing universities, government, site 

management and regulatory agencies, remediation companies, and research and 

development firms from around the world. Approximately 550 platform and poster 

papers were presented. 

Risk, Resource, and Regulatory Issues: Remediation of Chlorinated and 

Recalcitrant Compounds (C1-1). Nonaqueous-Phase Liquids: Remediation of 

Chlorinated and Recalcitrant Compounds (C1-2). Natural Attenuation: Chlorinated and 

Recalcitrant Compounds (C1-3). Bioremediation and Phytoremediation: Chlorinated and 

Recalcitrant Compounds (C1-4). Physical, Chemical, and Thermal Technologies: 

Remediation of Chlorinated and Recalcitrant Compounds (C1-5). Designing and 

Applying Treatment Technologies: Remediation of Chlorinated and Recalcitrant 

Compounds (C1-6). 

43 
Hirayama, K. and R. Hirano. 1970. Influences of high temperatures and residual 

chlorine on marine phytoplankton. Marine Biology 7:205-213. 

 Marine phytoplankton forms are frequently exposed to sudden biological changes 

such as rapid rise in water temperature and chlorine content of their environment, 

resulting from the use of sea water for cooling purposes by electric generators. The direct 

influence of these effluents, i.e., inhibitory effects of high temperature and residual 

chlorine on growth and photosynthesis of Chlamydomonas sp. and Skeletonema 

costatum, were investigated experimentally. Chlamydomonas sp. and S. costatum

exposed to high temperatures were affected in their growth from 43° and 35°C, 

respectively, by immersion of the respective cultures in a warm bath for 10 minutes. 

Treatment at high temperatures of 40 °C and 30° 35°C for 10 minutes, influenced their 

photosynthetic activities, which were completely inhibited immediately after 10 minutes 

exposure at 42° and 37 °C, respectively. S. costatum was killed by chlorine at a 

concentration of 1.5 2.3 ppm when exposed for exactly 5 or 10 minutes, while 

Chlamydomonas sp. was not irreversibly damaged even at 20 ppm chlorine or more with 

the same exposure period. These results lead to the conclusion that the high temperature 

of, and residual chlorine in, effuents from a power plant discharging into the open sea, 

should not cause great damage to marine phytoplankton in that area. 

Hoepner, T. and S. Lattemann. 2003. Chemical impacts from seawater desalination 

plants-- a case study of the northern Red Sea. Desalination 152(1-3): 133-140. 

 Seawater desalination facilities range from heavy coastal industry to small local 

plants, with the majority being either thermal multi-stage flash (MSF) or membranebased reverse osmosis (RO) plants. Irrespective of the process, pretreatment chemicals 

are added to the intake water to improve plant performance. Furthermore, corrosion 

cannot be entirely prevented and heavy metals add to the chemical load of the brines, 

which are discharged to the marine environment. For impact assessment, information 

about chemical loads as well as the sensitivity of the impacted ecosystem is required. 

Loads of selected chemicals were estimated for 21 plant locations in the Red Sea 

including the Gulf of Aqaba and Gulf of Suez. Locations were identified from the 2000 

IDA Worldwide Desalting Plants Inventory Report and localized on GIS data maps from 

the World Conservation Monitoring Center (WCMC). Their combined capacity exceeds 

1.5 million m/d, with approximately 1.2 million m/d from MSF and 0.38 million m/d 

from RO plants. Based on these figures, the daily chemical discharge amounts to 2,708 

kg chlorine, 36 kg copper and 9,478 kg antiscalants, when effluent concentrations of 0.25 

ppm, 0.015 ppm and 2 ppm are assumed, respectively. The sensitivity of the Red Sea and 

the Gulf of Aqaba to chemical loads is discussed and comparisons to the Arabian Gulf 

and the Mediterranean Sea are drawn. 

Holland, G.A., J.E. Laster, E.D. Neumann and W.E. Eldridge. 1960. Toxic effects of 

organic and inorganic pollutants on young salmon and trout. Research Bulletin No. 5, 

Department of Fisheries, State of Washington, Olympia, WA. 

 No abstract. 

44 
Holmes, N.J. 1969. The suppression of mussel fouling by chlorination. Symposium on 

Marine Biology, Research Lab Memorandum No. RD/L/M 269. Central Electricity 

Research Lab, Leatherhead, England. Pp. 35-38. 

 No abstract. 

Hose, J.E., T.D. King and J.S. Stephens, Jr. 1984. Effects of dechlorinated seawater on 

fish behavior. Marine Environmental Research. 11(1): 67-76. 

 Behavioral responses to chemically dechlorinated seawater were studied using 2 

species of marine fish associated with power plant discharge areas off Southern 

California. Opaleye (Girella nigricans) neither avoided nor were attracted to chlorinated 

seawater containing 0 multiplied by l mg/L total residual oxidants (TRO) which had been 

dechlorinated using equimolar or excess sodium thiosulfate. Blue-banded goby 

(Lythrypnus dalli) did not avoid dechlorinated seawater initially containing 0 multiplied 

by 1 or 0 multiplied by 9-1 multiplied by 0 mg/L TRO. Elimination of chlorine-produced 

oxidants by thiosulphate dechlorination resulted in 100% survival in both species. The 

toxicological implications of exposure to dechlorinated seawater are discussed. 

Hose, J., E., D. Di Fiore, H.S. Parker and T. Scarerrotta. 1989. Toxicity of chlorine 

dioxide to early life stages of marine organisms. Bulletin of Environmental 

Contamination and Toxicology 42(3): 315-319. 

 No information exists on chlorine toxicity to marine organisms. This study 

sought to obtain information on chlorine dioxide toxicity using an exposure schedule 

typical of generating stations which discharge into the marine environment. Early life 

history stages of a plant, invertebrate and fish were tested since these stages are most 

sensitive to toxicants and are most likely to be exposed to the effluent. 

Hoss, D.E., L.C. Coston, J.P. Baptist and D.W. Engel. 1975. Effects of temperature, 

copper and chlorine on fish during simulated entrainments in power plant condenser 

cooling systems. Pp. 519-527 in Environmental Effects of Cooling Systems at Nuclear 

Power Plants. Austria International Atomic Energy Agency, Vienna, Austria. 

 No abstract. 

Hoss, D.E., L.C. Clements and D.R. Colby. 1977. Synergistic effects of exposure to 

temperature and chlorine on survival of young of the year estuarine fishes. Pp. 345-355. 

In: F.J Vernberg, A. Calbrese, F.P Thurberg and W.B. Verbberg (eds). Physiological 

Responses of Marine Biota to Pollutants. Academic Press, Inc., New York, NY. 

 No abstract. 

45 
Huang, J., L. Wang, N. Ren, L.X. Li, S.R. Fun and G. Yang. 1997. Disinfection effect 

of chlorine dioxide on viruses, algae and animal planktons in water. Water Research 

31(3): 455-460. 

 In this paper, the disinfection effects of chlorine dioxide (ClO2) on some main 

viruses, algae and animal planktons in water and the influence of ClO2 on the inactivation 

of some microorganisms studied under various conditions such as the dose of 

disinfectant, contact time, and pH value, etc., were researched and reported. The effects 

and influence were compared with liquid chlorine, and results showed that the killing 

effect of ClO2 on algae is about the same as or better than that of liquid chlorine and the 

inactivation effect of ClO2 on viruses and animal planktons is more remarkable than that 

of liquid chlorine. The viruses were effectively killed off by ClO2 in a wider pH range. 

Moreover, the mechanism of viruses killed by ClO2 was tentatively investigated. Then, it 

is concluded that ClO2 is an excellent disinfectant as a substitute for liquid chlorine. 

Hughes, J.S. 1972. Acute toxicity of thirty chemicals to striped bass (Morone saxatilis). 

Paper presented at the Western Association of Game and Fish Commissioners. Salt Lake 

City, UT. 

 No abstract. 

Hughes, J.S. 1975. Striped bass (Morone saxatilis) culture investigations in Louisiana 

with notes of sensitivity of fry and fingerlings to various chemicals. Louisiana Wildlife 

and Fisheries Commission, Baton Rouge, LA: Fish Bulletin No. 15. 

 No abstract. 

Iyer, T.S.G. and P.R.G. Varma. 1994. Survival of Listeria monocytogenes in water 

subjected to different levels of chlorination. Fishery Technology. Society of Fisheries 

Technologists 31(1): 79-80. 

 The concentration of chlorine required to destroy all viable cells of Listeria 

monocytogenes present in the water used for seafood processing is studied. Based on 

these studies, it is recommended that the factories should chlorinate water supply to a 

reduced level of 10 ppm Cl2 giving minimum contact time of 5 minutes. 

James, W.G. 1966. Mussel fouling and the use of chlorine: Appendix I. In: Low Level 

Chlorination for the Control of Marine Fouling. Central Electricity Research 

Laboratories, Leatherhead, England. 

 No abstract. 

Jenner, H.A., C.J.L. Taylor, M. Donk and M. Khalanski. 1997. Chlorination by-products 

in chlorinated cooling water of some European coastal power stations. Marine 

Environmental Research 43(4): 279-293. 

46 
 Chlorination by-products (CBPs) are formed as a result of the chlorination of 

power station cooling water for anti-fouling purposes. Their production was studied at 10 

coastal power stations in the UK, France and The Netherlands. Three categories of CBPs 

were determined: trihalomethanes; haloacetonitriles; and halophenols. Bromoform was 

the CBP most abundantly present in the effluents of all 10 power stations. At a mean 

chlorine dosage of 0.5-1.5mg/L (as Cl2) the mean bromoform concentration was 16.32 

plus or minus 2.10 μg/litre. The CBP found in second highest concentrations was 

dibromoacetonitrile (DBAN) with mean concentrations of 1.48 ± 0.56 μg/litre. Other 

CBPs detected were dibromochloromethane, bromodichloromethane and 2,4,6-

trichlorophenol; concentrations of these three compounds were very low (<1 μg.litre). At 

those sites at which bromoform was measured in the dispersing effluent plume it was 

found to behave as a conservative parameter (significant direct correlation with plume 

ΔT). 

Johnson, A.G., T.D. Williams and C.R. Arnold. 1977. Chlorine-induced mortality of 

eggs and larvae of spotted sea trout (Cynoscion nebulosus) Transnational American 

Fisheries. Society 106: 466-469. 

 No abstract. 

Johnson, J.D., G.W. Inman, Jr. and T.W. Trofe. 1982. Cooling water chlorination: The 

kinetics of chlorine, bromine, and ammonia in sea water. Ncu/dese report. No pages. 

 The major inorganic reaction pathways for the chlorination of saline waters were 

measured by a variety of techniques including: (1) amperometric titration, (2) 

amperometric membrane covered electrode, (3) conventional kinetics methods for slow 

reactions, and (4) stopped-flow kinetics measurements with a micro-computer data 

acquisition system. The major reactions studied were: (1) the competitive reactions of 

ammonia and bromide ion with hypochlorous acid, (2) bromide oxidation by 

hypochlorous acid, (3) monochloramine formation in sea water, (4) monobromamine 

formation and subsequent disproportionation to form dibromamine, and (5) 

monochloramine oxidation of bromide to form bromochloramine. Reaction rates were 

determined in sodium chloride and sea water as a function of reactant concentration, pH, 

salinity, and ammonia concentration. 

Johnson, J.D. and R.G. Qualls. 1983. Kinetic model for the chlorination of power plant 

cooling waters. Department of Environmental Sciences and Engineering. Chapel Hill 

(USA), North Carolina University Report: 89 pp. 

 Concern over the environmental effects of chlorination has prompted efforts to 

minimize the amount of chlorine necessary to prevent fouling of power-plant condensers. 

Kinetic expressions are developed for the short-term reactions of chlorine consumption 

by organic substances in natural freshwater. These expressions were developed to use in 

a kinetic model to predict the free and total available chlorine discharged in cooling 

water. This model uses commonly available water-quality data. It assumes that most of 

the chlorine-consuming substances are: (1) NH3, (2) chloramine-forming organic-N, and 

47 
(3) humic substances. It uses the Morris-Wei model of chlorine-ammonia reactions. 

Chloramine formation from organic-N was represented by a model compound, 

glycylglycine. 

Jolley, R.L. 1984. Basic issues in water chlorination: a chemical perspective Water 

Chlorination Conference, Williamsburg, VA, USA, Department of Energy Report: 32 pp. 

 Results from selected research papers published since the last conference 

(Asilomar, 1981) are reviewed and summarized. Although much progress has been made 

in developing predictive models for chlorination discharges, additional data are needed 

for reaction rates and mechanisms over a wider temperature range, especially for 

transformation and decomposition reactions of chlorine and chlorine-produced oxidants. 

Significant advances have also been made toward understanding the formation of 

trihalomethanes and the chlorination of humic materials, and toward identification of 

chlorination products in cooling waters, paper industry effluents, and wastewaters; 

however, because of the vast number of chlorination products, much remains unknown in 

this area. Further research is especially needed to expand our knowledge concerning 

products from the water chlorination treatment of food materials because only very 

limited information is available. 

Junli, H., W. Li, R. Nanqi and M. Fang. 1994. Disinfection effect of chlorine dioxide on 

microorganism in water. Int. Conf. on Asian Water Technology '94, Raffles City 

(Singapore), 22-24 Nov 1994. No pagination. 

 In this paper, the disinfection effects of chlorine dioxide (ClO2) on some main 

bacteria, viruses, algae and animal planktons in water were researched by numerous 

experiments. Simultaneously, the influence of ClO2 on the inactivation of some 

microorganism was studied under various conditions such as the amount of the added 

disinfectant and the contact time, pH valve and other factors, and were compared with 

chlorine. The results showed that the inactivation effect of the ClO2 on bacteria and algae 

is similar or more closely related to the chlorine, and the inactivation effect of ClO2 on 

viruses and animal planktons is more remarkable than the chlorine. The bacteria and 

viruses were effectively killed off by using ClO2 in a very wide range of pH value. 

Moreover, the investigation of the mechanism of killing bacteria and viruses by ClO2 was 

tentatively undertaken. Then, we concluded that ClO2 is a excellent disinfectant as 

substitute for the chlorine. This study is divided into two pieces of writing, with the first 

piece of writing dealing with the disinfection effect of ClO2 on bacteria in water. 

Karaas, P. 1992. Zooplankton entrainment at Swedish nuclear power plants. Marine 

Pollution Bulletin 24(1): 27-32. 

 Studies on zooplankton entrainment with the cooling-water at four Swedish 

nuclear power plants demonstrated that mortality of crustaceans caused by mechanical 

damage and temperature elevation was low. Chlorination against fouling organisms, 

however, occasionally caused raised mortality. The greatest effect found on the 

zooplankton communities was caused by filter feeders attached to the walls of the 

48 
cooling-water systems. Up to about 50% of the plankton was filtered off, and the effect 

was positively related to the lengths of the cooling-water tunnels. Delayed mortality was 

low in most taxonomical groups except in the cladocerans. As the volumes of water 

passing through the cooling-water systems are very large, the effect on the zooplankton 

community was considered as severe. At one of the plants the estimated yearly average 

loss of crustacean zooplankton was some hundred tons. 

Karande, A.A., S.N. Gaonkar, R. Viswanathan and A.K. Sriraman. 1982. Bioassay of 

antifoulant chlorine. Indian Journal of Marine Sciences 11(2): 177-179. 

 Laboratory bioassays were conducted using laboratory cultured, young settling 

stages as well as adults of major fouling species, with a view to determining suitable 

chlorine dosage and chlorination cycle to mitigate biofouling. Fairly high concentration 

of chlorine was required to cause death of the larvae. It was, however, possible to 

prevent settlement by desensitizing the larvae with low levels of residual chlorine. To 

remove fouling by adults was very difficult. Residual chlorine as high as 15-20 ppm for 

a period of 60 hr would be necessary to remove settled biofouling. 

Ketelaars, H.A.M. and A.J. Wagenvoort. 1995. Control of Dreissena biofouling by the 

water storage corporation Brabantse Biesbosch. Aqua - Journal of Water Supply: 

Research and Technology 44 (1): 97-101. 

 Biofouling of intake, transport and distribution pipes can create major problems 

for the water industry, such as increased hydraulic resistance and energy costs, 

deterioration of taste and odour (decay of dead organisms) and promotion of bacterial 

regrowth and corrosion. The most notorious biofouling organism is the zebra mussel, 

Dreissena polymorpha. The Water Storage Corporation Brabantse Biesbosch (WBB) 

initially prevented Dreissena fouling by continuous chlorination of all intake and 

transport pipelines. After the discovery of undesirable disinfection byproducts (e.g., 

trihalomethanes) in the early 1970s, measures were taken to reduce the use of chlorine. 

The chlorine-dosing period was at first shortened by setting strict temperature limits, and 

since 1987 by restricting dosing to the growing season of Dreissena (occurrence of 

veliger larvae in zooplankton samples). In addition, other methods were used to reduce 

the total amount of chlorine. Chemical control of biofouling was terminated in places 

where the mussels could be removed easily by mechanical means. In some pipelines, 

flow rates turned out to be high enough to prevent biofouling, in others it sufficed to dose 

with chlorine intermittently. Another successful method was to create anoxic conditions 

in pipelines in order to remove the mussels. These control methods and the cessation of 

break-point chlorination resulted in a drop of the annual amount of chlorine used from 

730 tonnes in 1974 to 51 tonnes in 1993. 

Khalanski, M. and F. Bordet. 1978. Effects of chlorine on marine fauna. Journees de la 

Thermo-ecologie, Nantes (France), Nov 1979 Conference. No pagination. 

 In power plant cooling system, a seawater chlorination of 1 mg/L ClO- is not 

enough to detach mussels and to prevent mussels fixation. The solution to protect 

49 
pumping and cooling system should lead to an inhibition of mussels growth and a 

maintenance at a small size. 

Khalanski, M. and R. Delesmont. 1994. Chlorine by-products in sea water at the Penly 

nuclear power plant. Measurement survey in May 1993. Electricite de France, Clamart, 

51 pp. 

 The objective of the measurement survey conducted at the Penly nuclear power 

plant on May 27 and 28, 1993, was to determine the distribution of residual oxidants and 

volatile organo-halogenated compounds (THM) concentrations in the discharge plume 

when both units of the plants are carrying out chlorination. The data collected provide a 

mapping of the chemical types analyzed and will serve in calibration of a numerical 

model to simulate the evolution of these compounds in the discharge plume. During the 

two days of measurements, quantitative analyses were performed on samples taken by 

helicopter, once at high tide and twice at low tide with mean tidal coefficients. The 

chlorine injection level ranged from 0.84 to 0.92 mg/L (ppm) in unit 1 and from 0.64 to 

0.70 mg/L in unit 2. Residual oxidants were measured as Total Chlorine Equivalents 

using the colorimetric DPD method. Bromoform accounted for 97.8 % of the THM 

generated by chlorination. Three minutes after injection of hypochlorite in the discharge 

basin, bromoform reached 60% of its maximum concentration (29.23 μg/l). The 

maximum reaction yield of bromoform formation is 2.9 %. Three zones were defined 

according to their proximity to releases. In each zone, given the lack of precision in 

measurements, the concentration of residual oxidants found did not reach significant 

levels ≤0.03 mg/L. The bromoform concentration, on the contrary, reached measurable 

levels in each of the samples. Its distribution differs significantly from one zone to 

another: -release zone : 1.66±0.40 μg/l -nearby zone: 0.44±0.13μg/l - distant zone : 

0.26±0.10μg/l. Our analysis, which indicates a background level of the order of 0.1 μg/l 

for the entire studied area, raises the question of possible other sources of bromoform, 

independent of discharge from Penly. 

Kim, S.Y. and T.Y. Lee. 1989. The effects of Pollutants effluent from a steam-power 

plant on coastal bivalves. Contr. Inst. Marine Science of National Fisheries, University 

of Pusan: 223-242. 

 A study of the toxic effects on egg development and adult tissue of 3 marine 

bivalve species was performed using 4 main Pollutants from a steam-power plant. The 

bioassay consisted of 2 experiments, one which studied egg development of Crassostrea 

gigas and Mytilus edulis in various concentrations of Cu (CuSO4), Cl (NaOCl), Fe 

(FeCl2) and HCl and the other studied histological changes of adult bivalves, C. gigas, M. 

edulis) and Tapes philippinarum in various concentrations of the same Pollutants. 

Kristiansen, N.K., M. Froeshaug, K.T. Aune and G. Becher. 1994. Identification of 

halogenated compounds in chlorinated seawater and drinking water produced offshore 

using n-pentane extraction and open-loop stripping technique. Environmental Science & 

Technology 28(9): 1669-1673. 

50 
 The formation of byproducts in chlorinated seawater used for drinking water 

production on three oil installations was investigated with the use of n-pentane and openloop stripping extraction. The use of capillary gas chromatography (GC) with electron 

capture detection (ECD) and mass spectrometry (MS) revealed the presence of a great 

variety of bromine-substituted aliphatic and aromatic compounds in both chlorinated 

seawater and tap water. Tribromomethane and dibromoacetonitrile were found in tap 

water in the range of 19-27 μg/L and 0.9-1.6 μg/L, respectively, and these major 

byproducts were most efficiently extracted with n-pentane. However, the open-loop 

stripping technique was better suited for extraction of halogenated compounds present at 

the low nanograms per liter level, such as the iodinated trihalomethanes. When using npentane for extraction of chlorination byproducts, the formation of artifacts such as 3-

bromo-2-methyl-2-butanol and other halogenated C5 compounds was observed. These 

compounds were presumably formed from the reaction of excessive halogen with traces 

of olefins in the solvent. While ammonium sulfate only partly prevented the formation of 

the artifacts, sodium thiosulfate was efficient as a quenching agent and completely 

destroyed reactive halogen. 

Kurelec, B., S. Britvic, M. Rijavec, B. Sieben, N. Bihari, W.E.G. Mueller and R.K. Zahn. 

1983. Sea water chlorination: Creation of mutagenic byproducts. Rapp. P.-V. Reun. 

Ciesm 28(7): 135-136. 

 Recently it came to light how water chlorination generates carcinogens and 

mutagens. In addition to the formation of a small number of the mutagenic low 

molecular weight halogenated methanes (haloforms) there is also a potential to produce 

high molecular weight mutagenic substances. Some of these have been identified as 

derivatives from natural organic material dissolved in sea water. Coastal regions differ 

with regard to the quantity and quality of dissolved organic molecules due to the depth or 

due to the extent of environmental contamination. Consequently, the antibiofouling 

chlorination of the rather huge amounts of sea water used in cooling systems results in 

large amounts of mutagens. 

Lamon-Doherty Biossay Work. 1993. in Appendices Vol. III, Sect. III E. In: 

Environmental Report, Indian Point, No. 3, Consolidated Edison Co. of New York Inc., 

New York. 

 No abstract. 

"Laurer's Bioassy Work". 1973. in Appendices Vol. III, Sect. IIIC. In: Environmental 

Report, Indian Point No. 3, Consolidated Edison Co. of New York, Inc., New York, NY. 

 No abstract. 

Laurer, G.J. , W.T. Waller, D.W. Bath, W. Meeks, R. Heffer, T. Ginn, L. Zubarik, P. 

Bibko and P.C. Storm. 1974. Entrainment studies on Hudson River organisms. Pp. 37-

82. In: L.D Jensen (ed). Proceedings of the Second Workshop on Entrainment and 

51 
Intake Screening. Department of Geography and Environmental Engineering, The Johns 

Hopkins University, Baltimore, MD. 

 No abstract. 

Lassus, P., P. Maggi, C. Le Baut, M. Bardouil, G. Bocquene, L. Le Dean and P. Truquet. 

1981. The effects on chorination on the first larval stages of Palaemon serratus. Revue 

des Travaux de l'Institut des Peches Maritimes Nantes 45(1): 85-95. 

 In order to estimate the effects of chlorine used in cooling systems of electric 

power plants on zooplankton, some experiments have been realized with the four first 

larval stages of the prawn (Palaemon serratus Pennant). Chlorine injection was 

performed by constant adding at 16 and 20°C with time exposures of 5, 15 and 25 

minutes. If there is no detectable variation in sensitiveness of different stages, toxic 

effects of oxydants issued from chlorination can be observed (for both the two 

acclimation temperatures) at 15 minutes exposure with a LD 50 : 3 mg/L of chlorine. 

Lebreton, P. 1976. Ecological consequences of atomic power stations on the sea shore. 

Bulletin d'Ecologie 7(1): 33-59. 

 Ecological effects of giant nuclear power plants on the coastal environment are 

reviewed. Physical problems are discussed, e.g., the necessity of ecological mapping. 

Mechanical problems include the cooling systems and disturbance of marine micro- and 

macro-organisms. The heated discharges cause thermal pollution. Effects on dissolved 

chemicals and marine organisms, aquaculture and its limits are discussed. Chemical and 

radiochemical aspects discussed include synergistic Pollution, chlorination vs. fouling, 

acute or chronic radioactive effluents and concentration by food chains. Plurilogical 

categories can be distinguished on the basis of physical water turn-over; to these 

categories correspond various standards and recommendations for management of 

nuclear power plants in coastal zones. 

Liden, L.H. and D.T. Burton. 1977. Survival of juvenile Atlantic menhaden (Brevoortia 

tyrannus) and spot (Leiostomus xanthurus) exposed to bromine chloride and chlorinetreated estuarine waters. Journal Environmental Science Health A12: 375-388. 

 No abstract. 

Liden, L.H., D.T. Burton, S.L. Margrey and L.H Bongers. 1979. Comparative 

biotoxicity of bromochlorinated and chlorinated power plant condenser cooling waters to 

selected estuarine organisms. Association Southeastern Biology Bulletin 26: 83. 

 No abstract. 

Liden, L.H., D.T. Burton, L.H. Bongers and A.F. Holland. 1980. Effects of 

chlorobrominated and chlorinated cooling water on estuarine food-chain organisms. 

Journal of Water Pollution Control Federation 52: 173-183. 

52 
 Toxicities of chlorobrominated and chlorinated cooling waters to selected 

estuarine food chain organisms were investigated during an on-site power plant study. 

Survivals were similar among juvenile Atlantic menhaden (Brevoortia tyrannus) and spot 

(Leiostomus xanthurus) after exposures to BrCl and Cl2 treated condenser effluents. 

Juvenile American oysters (Crassostrea virginica) and brackish water clams (Rangia 

cuneata) had no halogen attributable mortalities after 15 day exposures. Similar survival 

reductions were recorded for copepods (Acartia tonsa) subjected to BrCl or Cl2 residuals 

(< 0.100 mg/L) for 24 h. eductions in O2 evolution (77-388%) and C-fixation (40-71%) 

rates and increased respiration rates (56-1276%) of entrained phytoplankton were 

recorded from both chlorobrominated and chlorinated samples. BrCl and Cl2 residuals 

appeared to have similar toxicities to estuarine organisms. 

Lou, K., E. Xu and J. Su. 1981. Studies on elimination of mussel growths in pipelines 

by chlorination. Oceanologia et Limnologia Sinica 12(2): 117-124. 

 No abstract. 

Lovell, C.R. 1999. Degradation of haloaromatic compounds by indigenous sediment 

microflora: biochemistry and molecular ecology. Report for 15 Jan 96-31 Mar 99. Dept. 

of Biological Sciences, South Carolina Univ., Columbia: 94 pp. 

 This study examined microbial communities in biofilms lining marine infaunal 

burrows with particular focus on marine bacteria able to degrade chlorinated aromatic 

compounds. These biofilms contain a diverse and abundant microbiota, occurring as 

single cells and in microcolonial formations. The majority of these organisms are 

potentially active. The burrow biofilm microbiota includes bacteria able to remove 

halogen atoms from the aromatic ring of haloaromatic compounds. At least some of 

these bacteria are amenable to isolation and can be cultivated in the laboratory. 

Reductively dechlorinating bacteria were enriched from burrows produced by both 

haloaromatic producing and non-producing marine infauna. It is clear from these results 

that marine dechlorinating bacteria are common in infaunal burrow structures relative to 

bulk sediments. Two isolates able to debrominate bromophenols were characterized and 

identified as novel strains of Propionigenium maris. Microscopic examination also 

revealed the potential for interspecific interactions among dehalogenating bacteria and 

other types of organisms. These interactions may include consortial metabolism of 

chloroaromatics. The study revealed and characterized an important and previously 

unrecognized niche for reductively dehalogenating bacteria in marine sediments. 

Infaunal burrow structures provide a source for new organisms with potential application 

in bioremediation processes. 

Macalady, D.L., J.H. Carpenter and C.A. Moore. 1977. Sunlight-induced bromate 

formation in chlorinated seawater. Science 195(4284): 1335-1337. 

 Chlorinated waters are being introduced into estuarine and coastal areas in 

increasing quantities. In such systems, the chlorine reacts with the natural bromide and 

ammonia to produce the highly toxic hypobromous acid, hypobromite ion, and 

53 
haloamines. Sunlight causes up to 50% conversion to bromate ion, which is persistent in 

natural waters and has an unknown toxicity. 

Marcy, B.C. 1973. Vulnerability and survival of young Connecticut River fish entrained 

at a nuclear power plant. Journal Research Board Canada 30: 1195-1203. 

 No abstract. 

Marcy, B.C., A.D. Beck and R.E. Ulanowicz. 1978. Effects and impacts of physical 

stress on entrained organisms. Pp. 135-188. In: J.R. Schurbel and B.C. Marcy, J. (eds). 

Power Plant Entrainment: A Biological Assessment. 

 No abstract. 

Margrey, S.L., D.T. Burton and L.B. Richardson. 1976. A preliminary study of chlorine 

toxicity to Atlantic menhaden (Brevoortia tyrannus) and hogchoker (Trinectes 

maculatus). Association Southeastern Biology Bulletin 23: 78. 

 No abstract. 

Margrey, S.L., D.T. Burton and L.W. Hall. 1981. Seasonal temperature and power plant 

chlorination effects on estuarine invertebrates. Archives of Environmental 

Contamination and Toxicology 10(6): 691-703. 

 The influence of seasonal temperatures (15-22°C) on chlorine (0.00, 0.15 and 

0.30 mg/L total residual chlorine), ΔT (2,6 and 10°C), and exposure duration (0.08, 2.0 

and 4.0 hr) interaction studies simulating power plant entrainment and effluent discharge 

conditions was evaluated for opossum shrimp, Neomysis americana and amphipods, 

Gammarus sp. Seasonal temperature was an important factor affecting the toxicity of the 

three treatment variables with both of these organisms. Mortality responses were greater 

at the higher acclimation temperature 

Marine Research Inc., Boston Edison Co., Nuclear Engineering Department, 800 

Boylston St., Boston, MA 02199, USA. 1976. Entrainment investigations and Cape 

Cod Bay ichthyoplankton study, September - December, 1975. Twelve-month summary 

for 1975. Marine ecology studies related to operation of Pilgrim Station. Semi-annual 

Report No.7. 

 Phytoplankton and zooplankton entrained at Pilgrim appear to be unharmed by 

entrainment, except during chlorination when damage is severe. Except during the 

months of June and July, when labrid eggs were particularly abundant near shore, 

ichthyoplankton densities in the Pilgrim discharge were generally lower than in the 

surrounding waters. It is estimated that during the summer months, 31% of the fish eggs 

entrained at Pilgrim fail to survive. Labrid eggs and the larvae of some spp are found in 

significantly greater abundance at the stations near Pilgrim than in the Bay as a whole, 

whereas for other spp, the converse is true. Analyses of the distribution of certain spp of 

54 
bivalve larvae and finfish eggs and larvae in Plymouth Bay tend to indicate that the 

Pilgrim station draws its cooling water primarily from an inshore water mass that fills 

Plymouth Bay and extends southeastward as a relatively narrow band along the shore. 

Results of the lobster larvae survey in Cape Cod Bay suggest the Cape Cod Canal was a 

primary source of larvae for the Bay during 1975. 

Markowski, S. 1959. The cooling water of power stations: A new factor in the 

environment of marine and freshwater invertebrates. The Journal of Animal Ecology 

28(2): 243-258. 

 1. A number of power stations were surveyed to see whether the cooling water 

system had any effect on the aquatic invertebrates found in fresh and sea water. 2. The 

samples of zooplankton were collected in the intake and in the outfall waters for 

quantitative and qualitative investigations. 3. Some thirty-five freshwater and sixty-two 

marine species were identified. 4. No detrimental effect on the organisms found was 

observed. They were alive after being circulated through the condensers. 5. No 

difference in fauna composition between the intake and the outfall was found from the 

qualitative and quantitative points of view. 6. Freshwater forms are able to live and 

reproduce in the cooling ponds of the power station. 7. The chemical and physical 

features of the cooling water were given. 

Marumo, K., F. Sato and Y. Ishikawa. 1992. Experimental study on acute effects of the 

combined exposure to temperature increase and chlorination upon the marine copepod 

Acartia omorii. Marine Biology. 114(2): 235-240. 

 The acute effects of combined exposure to temperature increase and chlorination 

on a neritic marine copepod, Acartia omorii (collected offshore of Onjuku, Japan in 

1982), were investigated in the laboratory. Continuous flow exposure and batch exposure 

modes were compared. Based on the results of continuous flow experiments, the 24-h 

median lethal concentration (24-h LC50, in mg/L) of total residual chlorine was estimated 

using the multiple regression equation below, with a multiple correlation coefficient of 

0.955: 24-h LC50 = 2.988-0.034 dT-1.611 log10 t where dT is temperature rise (°C) and t 

is exposure duration (minutes). In batch experiments, the predictive power of the 

multiple regression equation was reduced, probably due to variations in chlorine 

concentration during exposure duration. 

Masilamoni, G.J., K. Nandakumar, K. Satapathy and Azariah, J. 2002. Lethal and 

sublethal effects of chlorination on green mussel Perna viridis in the context of 

biofouling control in a power plant cooling water system. Marine Environmental 

Research 53(1): 65-76 

 Continuous chlorination is a widely followed cooling water treatment practice 

used in the power industry to combat biofouling. The green mussel Perna viridis is one 

of the dominant fouling organisms (> 70%) in the Madras Atomic Power Station. 

55 
Mattrice, J.S. 1977. Power-plant discharges: toward more reasonable effluent limits on 

chlorine. Resource Relations Nuclear Safety Oak Ridge National Lab., TN 18(6): 802-

819. 

 A method is presented for restricting chlorine in power-plant effluents to 

environmentally safe levels. Development of this method was stimulated by the 

controversy between electric utilities and regulatory agencies over the justification of the 

present universally applied limits. The scientific literature conclusively demonstrates the 

effects of physical, chemical, and biotic factors on chlorine toxicity. The method 

proposed includes these factors, to the extent currently possible, to set limits based on 

site-specific aquatic conditions and plant design and operation specifications. In these 

effluent limits, the organisms considered are those which are entrained into the plume or 

which maintain themselves within the plume during chlorination. In each instance the 

time course of exposure concentration is divided into small time intervals. Weighted 

mean concentrations for successively larger time intervals following initial exposure are 

then calculated. Exposures resulting from releases at various levels are compared either 

graphically or mathematically with acute and chronic mortality thresholds to find the 

highest discharge concentration that does not cause mortality. The thresholds are derived 

from existing toxicity data and are different for marine and freshwater organisms. This 

method is based on the latest literature available and can incorporate further data 

concerning chemistry, toxicity, and behavior as they become available. The method also 

is amenable to coupling with models of chemical dispersion and population dynamics to 

permit more complete analysis. This approach serves to permit use of chlorine for 

biofouling control at power plants, while ensuring that this use will not be inimical to the 

environment. 

Mattrice, J.S. and H.E. Zittel. 1976. Site-specific evaluation of power plant chlorination. 

Journal of Water Pollution Control Federation 48(10): 55-65. 

 The literature on chlorine toxicity to aquatic organisms is critically reviewed 

regarding methodology. Extant toxicity data for marine and freshwater organisms are 

summarized using log concentration versus log time plots of median tolerance (50%) 

levels. Acute and chronic toxicity thresholds are approximated by enclosing these data 

points and then shifting the resultant lines. The chronic toxicity threshold for fresh water 

is 0.0015 mg/L, while that for salt water is 0.02 mg/L. A site-specific procedure for 

evaluating chlorination schemes at a power plant is proposed based on comparison of 

dose-times expected in its discharge plume with the above toxicity thresholds. Dosetimes higher than the threshold are considered to yield mortality while those below it are 

not toxic. An analysis of a hypothetical marine-based power plant is presented. 

McLean, R.I. 1972. Chlorine tolerance of the colonial hydroid Bimeria franciscana. 

Chesapeake Science 13: 229-230. 

 The euryhaline colonial hydroid Bimeria franciscana was experimentally exposed 

to various concentrations of chlorine to determine the effect of power plant chlorination 

on growth. Colonies attached to suspended nylon lines were exposed to 0.0, 1.0, 1.5, 2.5, 

3.5, 4.5 ppm total chlorine for 1 and 3 hr, and returned to their natural habitat. Growth 

56 
occurred in all colonies and was slightly inhibited in colonies exposed to the higher 

chlorine concentrations. 

McLean, R.I. 1973. Chlorine and temperature stress on estuarine invertebrates. Water 

Pollution Control Federation 45(5): 837-841. 

 Five species of estuarine invertebrates subject to entrainment in the cooling water 

system of a steam electric station were exposed experimentally to chlorine and 

temperature stresses simulating plant operations. Estuarine water was injected with 

chlorine gas to achieve a final concentration of 2.5 mg/L total chlorine residual. This 

concentration effected as high as 80% population mortality in the barnacle nauplii 

Balanus species and 90% in the copepod Acartia tonsa during a 5-minute exposure. 

Temperature elevations of 10°F (5.5°C) and 20°F (11°C) for 3 hours had no significant 

effect on population mortality. 2 amphipods, Gammarus species and Melita nitida, and 

one species of shrimp, Palaemonetes pugio, exhibited greater tolerance to the same stress 

conditions. 

Mehrtens, G. and F. Laturnus. 1999. Mixed function oxidase dependent 

biotransformation of polychlorinated biphenyls by different species of fish from the 

North Sea. Chemosphere 38(13): 2995-3002. 

 Mixed function oxidase (MFO) dependent biotransformation of polychlorinated 

biphenyls (PCBs) was measured in three different fish species from the North Sea. Liver 

microsomes of plaice (Pleuronectes platessa), dab (Limanda limanda) and cod (Gadus 

morhua) were isolated and incubated with different PCB congeners. Metabolites of 

PCBs were identified as monohydroxylated PCBs. Hydroxylation took place in meta- or 

para-position of the PCB molecules, preferential in para-position. Metabolization and 

metabolic rates depended on the substitution pattern of PCBs and the degree of 

chlorination. Biotransformations were also species dependent. The flatfish dab and 

plaice exhibited higher metabolic rates than cod. 

Meldrim, J.W. and J.A. Flava, Jr. 1977. Behavioral avoidance responses of estuarine 

fishes to chlorine. Chesapeake Science 18: 154-157. 

 No abstract. 

Meldrim, J.W., J.J. Gift and B.R. Petrovsky. 1974. The effect of temperature and 

chemical Pollutants on the behavior of several estuarine organisms. Bulletin No. 11, 

Ichthyological Associates, Inc., Ithaca, NY. 

 No abstract. Link to full-text: http://estuaries.olemiss.edu/cgibin/est/public_access_search4.pl 

Middaugh, D.P. and W.P. Davis. 1975. Impact of chlorination processes on marine 

ecosystems. Water quality criteria research of the U.S. Environmental Protection 

57 
Agency. Proceedings of an EPA-sponsored symposium on marine, estuarine and freshwater quality - presented at the 26th annual meeting of AIBS, August 1975. No. 600/3-

76-079, [Ecol. Research Ser.USEPA]. Jul 1976. No pagination. 

The use of chlorine as a disinfectant and antifouling agent is reviewed. Chemical 

reactions of chlorine in aquatic environments are discussed, with particular emphasis on 

the formation of halogenated organic constituents in freshwater and marine systems. 

Studies of the effect of chlorinated sewage effluents and cooling water from generating 

stations on marine organisms and ecosystems are summarized. 

Middaugh, D.P., J.A. Couch and A.M. Crane. 1977a. Responses of early life history 

stages of the striped bass, Morone saxatilis to chlorination. Chesapeake Science 18: 141-

153. 

 The toxicity of total residual chlorination (TRC) to early life stages of the striped 

bass, Morone saxatilis, was determined using percent embryo hatchability, incipient LC50

bioassays, histopathology, and avoidance responses. Beginning 8 to 9 hours after 

fertilization, developing embryos were exposed continuously to TRC in flowing water at 

1.0-3.0 ppt salinity and 18 +/- 1 o

C. Fifty-six% of the control group (no TRC exposure) 

hatched. None of the embryos exposed to a measured TRC concentration of 0.21 mg/L 

hatched. Only 3.5% of the embryos exposed to 0.07 mg/L TRC and 23% of those 

exposed to 0.01 mg/L TRC hatched. Incipient LC50 bioassays were used to determine the 

sensitivity of 2-, 12- and 30-day-old striped bass to concentrations of TRC in flowing 

water (1.0-3.0 ppt salinity at 18±1o

C). The estimated incipient LC50 was 0.04 mg/L TRC 

for 2-day-old prolarvae, 0.07 mg/L for 12-day-old larvae and 0.04 mg/L for 30-day-old 

juveniles. Histological examination of 30-day-old juveniles which survived exposure in 

the incipient LC50 bioassay indicated gill and pseudobranch damage for fish exposed to 

0.21 to 2.36 mg/L TRC. Statistical analysis of avoidance tests conducted at 1.0-3.0 ppt 

salinity and 18 ± 1 o

C with 24-day-old larvae showed significant (X2, p < 0.05) and 

reproducible avoidance responses to measured TRC concentrations of 0.79-0.82 mg/L 

and 0.29-0.32 mg/L. No avoidance was indicated at TRC concentrations of 0.16-0.18 

mg/L. 

Middaugh, D.P., J.A. Couch and A.M. Crane. 1977b. Toxicity of chlorine to juvenile 

spot, Leiostomus xanthurus. Water Research 11: 1089-1096. 

 The sensitivity of juvenile spot, Leiostomus xanthurus, to total residual chlorine 

(TRC) in flowing sea-water was investigated. Incipient LC50 bioassays, histopathology, 

avoidance tests and the combined effect of thermal stress and TRC were used to assess 

sensitivity. Estimated incipient LC50 values were 0.12 mg/L TRC at 10°C and 0.06 mg/L 

TRC at 15°C. Histological examination of spot used in the incipient LC50 bioassay at 

15°C and sacrificed while alive indicated pseudobranch and gill damage occurred in 

individuals exposed to a measured TRC concentration of 1.57 mg/L. Spot exposed to 

lower concentrations of TRC, 0.02-0.06 mg/L at 15°C and sacrificed alive showed no 

consistent tissue damage. Spot demonstrated temperature dependent avoidance responses 

to TRC. At 10°C, a concentration of 0.18 mg/L was required for significant (X2

; P < 

0.05) avoidance; at 15 and 20°C, spot showed significant avoidance of TRC 

concentrations as low as 0.05 mg/L. Simultaneous exposure of spot to thermal stress (5, 

58 
10 or 13°C above the acclimation temperature of 15°C) at measured TRC concentrations 

of 0.05 0.07 and 0.34–0.52 mg/L demonstrated a significant, (Z2

) with Yates correction, 

P < 0.05) increase in sensitivity to TRC with increased temperature and exposure times 

for some of the groups tested. 

Middaugh, D.P., J.M. Dean, R.G. Domey and G. Floyd. 1978. Effect of thermal stress 

and total residual chlorination on early life stages of the mimmichog, Fundulus 

heteroclitus. Marine Biology 46: 1-8. 

 Effects of simultaneous short-term (7.5-60 minutes) thermal stress (24-34°C) and 

total residual chlorination (0.05-1.0 mg/L) on specific development stages of the 

mummichog F. heteroclitus, were investigated. For the embryonic stages, total number 

of successfully hatched larvae was used as the criterion to measure effect. For larval 

stages, survival 24 h exposure was used. In embryonic stages, temperature was the most 

important main variable. Only 1 embryonic stage (gastrula) was confounded by 2ndorder interactions (temperature x duration of exposure x total residual chlorination). Both 

0-day and 7 day old larval stages showed significant higher-order interactions for all 

combinations of test parameters, suggesting the presence of synergistic effects of the 3 

main experimental variables. 

Morgan, R.P. and R.G. Stross. 1969. Destruction of phytoplankton in the cooling water 

supply of a steam electric station. Chesapeake Science 10: 165-171. 

 Rates of photosynthesis were used to evaluate the response of phytoplankton 

when passed through the cooling system of a power generating facility. Photosynthesis 

was measured as the uptake of carbon. A factorial design permitted evaluation of heat 

input, total effect of passage, and degree of recovery. Six experiments measured 

response at a fixed time of day and at various times in a daily cycle. An increase in 

temperature of approximately 8.0 oC stimulated photosynthesis when natural water 

temperatures were 16 oC or cooler and inhibited photosynthesis when natural water 

temperatures were 20 o

C or warmer. Passage through the cooling system resulted in 

further inhibition when the water was warm and nullified thermal stimulation when the 

water was cool. No recovery was observed within a four hour period following passage. 

Photosynthesis in cooled effluent water was either unaffected or actually suppressed. 

The existence of daily synchrony in the algae was shown by a daily maximum of thermal 

stimulation at 0900. The rhythm is considered inherent and only amplified by warming. 

Morgan, R.P. and R.D. Prince. 1977. Chlorine toxicity to eggs and larvae of five 

Chesapeake Bay fishes. Transnational American. Fish Society 107: 636-641. 

 Eggs and larvae of white perch (Morone americana), striped bass (Morone 

saxatilis) and blueback herring (Alosa aestivalis) and eggs of Atlantic silversides 

(Menidia menidia) and tidewater silversides (M. beryllina) were exposed to various 

residual chlorine levels for preestablished periods. Almost all LC50 values fell between 

0.20-0.40 ppm of total residual chlorine for eggs, and between 0.20-0.32 ppm for larvae. 

59 
Age-related effects in sensitivity to chlorine were observed. Abnormal larvae issued 

from blueback herring eggs exposed to low chlorine concentrations. 

Morgan, R.P. and R.D. Prince. 1978. Chlorine effects on larval development of striped 

bass (Morone saxatilis), white perch (M. americana) and blueback herring (Alosa 

aestivalis). Transnational American Fish. Society 107: 636-641. 

 Eggs of striped bass (M. saxatilis), white perch (M. americana) and blueback 

herring (A. aestivalis) were exposed to a series of total residual Cl (TRC, a biocide) 

concentrations until hatch occurred. Larval development of the 3 species was inhibited 

by increasing TRC concentrations. Length of larval fish at hatch decreased with 

increasing TRC concentrations and the production of abnormal blueback herring larvae 

increased with increasing TRC concentrations. 

Morgan, R.R., D.A. Flemer, L.A. Noe, J.V. Rasin and R.A. Murtagh. 1974. Biochemical 

studies of entrained phytoplankton at the Morgantown Maryland Power Plant. Pp. 165-

168. In: L.D Jensen (ed). Proceedings of the Second Workshop on Entrainment and 

Intake Screening. The Johns Hopkins University, Department of Geography and 

Environmental Engineering, Baltimore, MD. 

 No abstract. 

Morris, R.J., F. Culkin, A.P.M. Lockwood and A.C. Jensen. 1983. Effect of chlorination 

on the gill lipids of the mussel Mytilus edulis (L.). Chemistry and Ecology 1(3): 173-189. 

 Experiments are described which involve the chlorination of standard solutions of 

lipids and populations of the mussel, Mytilus edulis (L). The object was to test the 

hypothesis that chlorine will bind to unsaturated lipids. Evidence is presented that 

chlorine and aqueous chlorinated species rapidly and readily form addition compounds 

with unsaturated lipids in vivo as well as in vitro. The relevance of these findings to 

possible environmental effects of chlorination is discussed. 

Mount, D.R. and W. Quast. 1993. Assessing toxicity of varying major ion concentrations 

to marine organisms. 14th Annual Meeting (SETAC) - Ecological Risk Assessment: 

Lessons Learned? -- Pensacola Conference. No pagination. 

 Recent regulatory developments have required that produced waters discharged in 

the Gulf of Mexico be monitored for toxicity to marine organisms. While produced 

water may contain a variety of indigenous and introduced chemicals, virtually all have 

moderate to high concentrations of major ions (e.g., sodium, chloride). Although 

seawater is also rich in these ions, excessive (or insufficient) salinity can cause toxicity to 

marine organisms. Perhaps more importantly, toxicity to marine organisms can be 

caused by deviations from normal ion ratios (relative to seawater) even if the total salinity 

is within organism tolerances. To provide a better understanding of marine organism 

responses to variations in major ion concentrations, we conducted a series of laboratory 

experiments to quantify the responses of mysid shrimp (Mysidopsis bahia) and 

60 
sheepshead minnows (Cyprinodon variegates) to modifications of normal seawater 

chemistry. Acute testing included both increasing and decreasing the concentrations of 

individual ions relative to seawater, as well as altering total salinity. Results show these 

organisms can be adversely affected by this altered chemistry and their sensitivity is 

dependent upon the individual ions that are manipulated. Results from these studies are 

being incorporated into an overall strategy for evaluating the influence of major ion 

chemistry on produced water toxicity tests. (DBO) 

Muchmore, D. and D. Epel. 1973. The effects of chlorination of wastewater on 

fertilization in some marine invertebrates. Marine Biology 19(2): 93-95. 

 Unchlorinated domestic sewage was a relatively weak inhibitor of external 

fertilization in 3 marine invertebrates (Strongylocentrotus purpuratus, Urechis caupo, 

Phragmatopoma californica). Chlorinated sewage was a potent spremicide, active in 

inhibiting fertilization in concentrations of available chlorine as low as 0.05 ppm. 

Sodium hypochlorite in seawater duplicated the effect, and excess sodium thiosulfate 

terminated it. The possibility of chlorine disinfection affecting reproductive success in 

the vicinity of outfalls is discussed. 

Mulford, R.A. 1974. Morgantown entrainment Part IV, phytoplankton taxonomic 

studies. Pp. 169-175. In: L.D Jensen (ed). Proceedings of the Second Workshop on 

Entrainment and Intake Screening. The Johns Hopkins University, Department of 

Geography and Environmental Engineering, Baltimore, MD. 

 No abstract. 

Nalewajko, C. and T.G. Dunstall. 1994. Miscellaneous Pollutants: Thermal effluents, 

halogens, organochlorines, radionuclides. Advances in Limnology Report: 7 pp. 

 Phytoplankton may become entrained in water intake of power plants and pass 

through the cooling system where they are exposed to a sudden increase in temperature. 

Photosynthetic rates are used to assess the impact of entrainment on phytoplankton. 

From these studies it became apparent that in the absence of a chlorination treatment, at 

low ambient water temperature, photosynthesis was either unaffected or stimulated after 

passage of the algae through the cooling system. The photosynthetic response 

encountered in these various entrainment studies depends for the most part on total 

temperature attained during station passage. 

Nicholson, B.C., G.R. Shaw, J. Morrall, P.J. Senogles, T.A. Woods, J. Papageorgiou, C. 

Kapralos, W. Wickramasinghe, B.C. Davis, G.K. Eaglesham and M.R. Moore. 2003. 

Chlorination for degrading saxitoxins (paralytic shellfish poisons) in water. 

Environmental Technology 24(11): 1341-1348. 

 Chlorination was investigated as a treatment option for degrading and thus 

removing saxitoxins (paralytic shellfish poisons, PSPs) produced by cyanobacteria (bluegreen algae) from water. It was found to be effective with the order of ease of 

61 
degradation of the saxitoxins being GTX5 (B1) similar to dcSTX > STX > GTX3 similar 

to C2 > C1 > GTX2. However the effectiveness of chlorine was pH dependent. 

Degradation as a function of pH was not linear with the degree of degradation increasing 

rapidly at around pH 7.5. At pH 9 > 90% removal was possible provided a residual of 

0.5 mg/L free chlorine was present after 30 minutes contact time. The more effective 

degradation at higher pH was unexpected as chlorine is known to be a weaker oxidant 

under these conditions. The more effective degradation, then, must be due to the toxins, 

which are ionisable molecules, being present in a form at higher pH which is more 

susceptible to oxidation. The feasibility of using chlorine to remove saxitoxins during 

water treatment will therefore depend strongly on the pH of the water being chlorinated. 

Degradation may be improved by pH adjustment but may not be a practical solution. 

Although saxitoxins were degraded in that the parent compounds were not detected by 

chemical analysis, there is no indication as to the nature of the degradation products. 

However, acute toxicity as determined by the mouse bioassay was eliminated. 

Oceanographique, J.D.R. 1998. Coastal zone monitoring and medium to long term 

forecasting. Journal Research Oceanography 23 (1): 21-35. 

 Several methodologies and tools both used by Japan and France for coastal 

monitoring and forecasting are presented. Chemical treatment (sea water chlorination), 

measurements of time-weighted average concentration of benzo(a)pyrene in marine 

environment, telluric inputs, macrobentic communities responses to organic load, 

annelids polychaetes responses to organic Pollution, and quantitative measurement of 

coral biomass are given as experiments. 

Opresko, D. 1982. Review of open literature on effects of chlorine on aquatic 

organisms. T. Oak Ridge National Lab., Government Reports Announcements. Issue 6: 

320 pp. 

 The pre-1980 open literature on the chemistry and Biological effects of chlorine 

in marine, freshwater, and estuarine systems is reviewed with special emphasis placed on 

the potential impacts of power plant chlorination practices. Information is provided on 

aqueous chlorine chemistry including the types and relative concentrations of residual 

oxidants formed under varying environmental conditions; the stability, persistence, and 

reactivity of these oxidants; and their potential for forming halogenated organic 

compounds. Analytical methods for differentiating and measuring chlorine-produced 

residual oxidants are discussed in a separate section. Information on Biological effects, 

derived mainly from the results of laboratory bioassays, consists primarily of acute 

toxicity data, but also included are discussions of Biological and environmental factors 

affecting species' tolerance levels; sublethal effects on reproduction, growth, behavior, 

and physiological processes such as respiration and osmoreg. 

Opresko, D. 1983. Effects of chlorine on aquatic organisms: A review of the literature 

with special reference to cooling water chlorination. T. Oak Ridge National Lab., 

Government Reports Announcements. Issue 22: 318 pp. 

62 
 The pre-1980 open literature on the chemistry and Biological effects of chlorine 

in marine, freshwater, and estuarine systems is reviewed with special emphasis placed on 

the potential impacts of power plant chlorination practices. Information is provided on 

aqueous chlorine chemistry including the types and relative concentrations of residual 

oxidants formed under varying environmental conditions; the stability, persistence, and 

reactivity of these oxidants; and their potential for forming halogenated organic 

compounds. Analytical methods for differentiating and measuring chlorine-produced 

residual oxidants are discussed in a separate section. Information on Biological effects, 

derived mainly from the results of laboratory bioassays, consists primarily of acute 

toxicity data, but also included are discussions of Biological and environmental factors 

affecting species' tolerance levels; sublethal effects on reproduction, growth, behavior, 

and physiological processes such as respiration and osmoreg. 

Pacheco Ruiz, I., A. Galvez-Telles, J.A. Zertuche-Gonzalez and J.L. Pech. 1997. Effect 

of using commercial chlorine (HClO3Na) on the growth of epiphyte algae and their host 

Gigartina pectinata Daw. (Gigartinales, Rhodophyta). Ciencias Marinas 23(3): 395-401. 

 Different concentrations of commercial chlorine were used to control the growth 

of epiphytes on cultured red algae Gigartina pectinata. Results showed that the better 

concentration was 100 microliters per liter which was lethal for all the epiphytes but 

didn't affect the growth of G. pectinata. 

Peck, B.B. and R.S. Warren. 1978. Nitrate reduction activity and primary productivity 

of phytoplankton entrained through a nuclear power station on northeastern Long Island 

Sound. Pp. 392-409. In: J.H. Thorp and J.W. Gibbons (eds). Energy and 

Environmental Stress in Aquatic Systems. DOE Symposium Series 48 CONF-771114. 

 No abstract. 

Peres, G. 1988. The use of chemicals in the marine environment. Oceanis. Serie de 

documents oceanographiques. Devenir des Pollutants Chimiques en Milieu Marin, Brest 

(France), 26-27 Jan. Conference Proceedings. Vol.14(6): No pagination. 

 The protection of industrial refrigeration circuits has been considered first. 

Chlorination has long been employed and the efficiency of this method and its possible 

action on the marine environment is assessed. Secondly we are concerned with 

antifouling paint used to protect hulls and marine culture equipment. A survey of the 

principal biocides has been made with special reference to the organostannic compounds. 

Past experience and knowledge of effects on the environment and fish farms point to the 

need to regulate the use of these chemicals. 

 

Perissinotto, R. and T. Wooldridge. 1989. Short-term thermal effects of a powergenerating plant on zooplankton in the Swartkops Estuary, South Africa. Pubblicazioni 

della Stazione zoologica di Napoli I: Marine Ecology 10(3): 205-219. 

63 
 The short-term effect of elevated water temperatures (Δt = 5-10°C after passing 

through the cooling circuit of an electricity generating plant) on plankton in a warm 

temperate estuary, South Africa, was investigated. Phytoplankton entrained on the flood 

tide was more severely affected than that entrained on the ebb, but chlorination of cooling 

water was probably a major factor affecting phytoplankton assemblages. Abundance of 

zooplankton of marine origin was significantly reduced after passing through the cooling 

circuit. The effect of thermal stress on the euryhaline zooplankton was not clear. 

Pinkney, A. 1992. Summary of recent information on biofouling control at power plants. 

- Final report. I. Versar, Columbia, MD. Annapolis, Maryland Power Plant Research 

Program: 26 pp. 

 Control of fouling organisms at power plants requires large investments by 

utilities. This report provides an overview of power plant biofouling and control 

techniques, a discussion of research and technologies investigated nationally, and a 

description of studies conducted at Maryland power plants. The major biofouling control 

techniques currently under investigation are: (1) using sodium bromide and bromine 

chloride in place of and in addition to chlorine; (2) conducting chlorine minimization 

studies and targeted chlorination trials to use chlorine more effectively; (3) using 

mechanical cleaning as an alternative method of controlling fouling; and (4) applying 

non-toxic coatings to intake structure An important issue is the increased use of bromine 

and mollucides at power plants and the establishment of discharge limits. Chlorine 

minimization studies are in progress or have been completed recently at several Maryland 

power plants but results are not yet available. Targeted chlorination is a promising 

technique for reducing chlorine usage. At some plants, mechanical cleaning has 

controlled condenser tube fouling and is used in place of biocides. Several trials have 

demonstrated the effectiveness of non-toxic coatings for reducing fouling on intake 

structures; however, cost savings are not yet apparent. 

Public Service Electric and Gas Company. 1978. Temperature and chemical avoidance 

studies Sect. 4.2.3. in Annual Environmental Operating Report , Salem Nuclear 

Generating Station- Unit 1, 1977 Report. Newark, NJ. 

 No abstract. 

Rainville, R.P., B.J. Copeland and W.T. McKean. 1975. Toxicity of kraft mill wastes to 

an estuarine phytoplankter. Journal Water Pollution Control Federation 47(3): 487-503. 

 A study was conducted to evaluate the toxicity of kraft mill wastes to 

Coccochloris elebans. Through a series of bioassays, the effect of these wastes on the 

growth potential of this organism was determined. Untreated and primary treated 

effluents were toxic to C. elebans in concs as low as 2.5%. At mills using aeration or 

long term stabilization as secondary treatment, no appreciable final toxicity was found. 

Brown water was the most important stream in determining the toxicity of the pulp mill 

wastewater and was toxic in all concs > 14.4%. The most toxic streams within the bleach 

plant originate from the chlorination, chlorine dioxide, and first caustic extraction stages. 

64 
Rajagopal, S. 2001. Can intermittent chlorination really control mussel fouling in 

industrial cooling water systems? 11th International Conference on Aquatic Invasive 

Species, Alexandria, VA (USA), 1-4 Oct 2001. (World Meeting Number 000 5699). 

 Availability: 11th International Conference on Aquatic Invasive Species, 1027 

Pembroke Street East, Suite 200, Pembroke, ON K8A 3M4, Canada; phone: 613-732-

7068; fax: 613-732-3386; URL: www.aquatic-invasive-species-conference.org 

 No abstract. 

Rajagopal, S., K.V.K. Nair, G. Van Der Velde and H.A. Jenner. 1997a. Response of 

mussel Brachidontes striatulus to chlorination: an experimental study. Aquatic 

Toxicology 39(2): 135-149. 

 Though the brackish-water mussel, Brachidontes striatulus (Hanley) (syn. 

Modiolus striatulus) is an important fouling animal in power station cooling circuits, 

there are no published studies on the tolerance of this species to chlorination. Mortality 

pattern as well as physiological behavior (byssus thread production, foot activity index 

and filtration rate) of three different size groups (7, 18 and 25mm shell length) of B. 

striatulus, were studied at different chlorine concentrations (0-5 mg/L). At 1 mg/L 

chlorine residual, 7mm size group mussels showed 100% mortality in 468 h (20 days) 

and 25mm size group mussels in 570 h (24 days). At relatively high levels of chlorine 

residuals (5 mg/L), 100% mortality in 7 and 25mm size groups took 102 and 156h, 

respectively. The exposure time to chlorine required for 100% mortality of B. striatulus

is much lower than that reported for some other common fouling mussels. Chlorine 

concentration significantly affected the mean time of death for all size groups of mussels. 

All size groups showed progressive reduction in physiological activities such as filtration 

rate, foot activity index and byssus thread production when chlorine residuals were 

increased from 0 to 1.5 mg/L. Reduction in physiological activities of mussels was 

strongly correlated with the concentration of chlorine. 

Rajagopal, S., G. Van Der Velde and H.A. Jenner. 1997b. Shell valve movement 

response of dark false mussel, Mytilopsis leucophaeta, to chlorination. Water Research 

31(12): 3187-3190. 

 Shell valve movements of fouling mussel, Mytilopsis leucophaeta, have been 

studied in the presence of chlorine, using a mussel monitor. Data showed increasing shell 

valve closure with increasing chlorine concentration. Shell opening rates of M. 

leucophaeta at control experiments (0 mg/L residual chlorine) were about 10 times more 

than those at 1 mg/L residual chlorine. Continuous dosing of 0.75 mg/L residual chlorine 

is required before shell movements are critically affected. Since current environmental 

stipulations do not permit this, a level of 0.5 mg/L has to be used continuously during 

settlement periods of M. leucophaeta for their control. The results also indicate that M. 

leucophaeta is more tolerant to chlorine than other mussel species. 

65 
Rajagopal, S., G. Van Der Velde and H.A. Jenner. 2002a. Does status of attachment 

influence survival time of zebra mussel, Dreissena polymorpha, exposed to chlorination? 

Environmental Toxicology and Chemistry 21(2): 342-346. 

 Mussels colonize cooling water circuits of power stations by attaching themselves 

to the pipe or conduit walls using byssus threads. Once manually detached, they quickly 

try to reattach by producing new byssus threads. In many published reports on 

antifouling bioassays, the test specimens are exposed to the biocide in an unattached 

state. These mussels, while trying to reattach, are likely to expose themselves more 

frequently to the toxic compound when compared to firmly attached mussels. The results 

of the assay, therefore, could vary, depending on the status of the mussels used. In this 

paper, we test the hypothesis that the status of attachment could influence the toxicity 

response of mussels and show that byssally attached zebra mussel, Dreissena polymorpha

(Pallas), is more resistant to chlorine than unattached ones. An average increase of 27% 

in the survival time was observed for attached mussels over unattached ones in the 

chlorine concentration range of 0.25 to 3 mg/L. It is conclusively shown that the increase 

in sensitivity of the unattached mussels was related to an increase in the byssal activity, 

quantified presently as the byssogenesis index. The results indicate that future laboratory 

toxicity experiments involving mussels should be carried out using byssally attached 

ones. 

Rajagopal, S., G. Van Der Velde and H.A. Jenner. 2002b. Effects of low-level 

chlorination on zebra mussel, Dreissena polymorpha. Water Research 36(12): 3029-

3034. 

 Mortality pattern of different size groups (5-20 mm shell lengths) of the zebra 

mussel Dreissena polymorpha (Pallas), was studied in the laboratory under different 

chlorine concentrations (0.25-3 mg/L). Results showed that exposure time for 100% 

mortality of mussels significantly decreased with increasing chlorine concentration. For 

example, mussels in the 10 mm size group exposed to 0.25 mg/L chlorine residual took 

1080 h to reach 100% mortality whereas those exposed to 3 mg/L chlorine took 252 h. 

All size groups (between 5 and 20 mm shell length) took identical exposure time to reach 

100% mortality at given chlorine concentration (between 1 and 3 mg/L). The effect of 

acclimation temperature on D. polymorpha mortality in the presence of chlorine was 

significant. For example, 1026 h is required to reach 95% mortality using 0.5 mg/L 

residual chlorine at 10°C, compared to 456 h at 0.50 mg/L chlorine and 25°C. Resistance 

of D. polymorpha to chlorine appeared to be lower than that for other mussel species in 

The Netherlands viz., Mytilus edulis L. and Mytilopsis leucophaeata (Conrad). The 

present study also suggests that 100% mortality data for European populations of D. 

polymorpha at different chlorine concentrations are similar to those for the North 

American populations. 

Rajagopal, S., G. Van Der Velde, M. Van Der Gaag, H.A. Jenner. 2002c. Laboratory 

evaluation of the toxicity of chlorine to the fouling hydroid Cordylophora caspia. 

Biofouling. 18(1): 57-64. 

66 
 The brackish water hydroid, Cordylophora caspia (Pallas) (syn. Cordylophora 

lacustris Allman) is an important fouling animal in industrial cooling water systems. 

However, there are no published studies regarding the response of this species to 

chlorination, which is a widely used antifouling method. The effects of different chlorine 

concentrations (0.1-1 mg/L total residual chlorine) on growth rate (k) of C. caspia were 

studied in the laboratory. The results show that chlorine is effective at relatively low 

concentrations (above 0.1 mg/L residual chlorine). The growth rate of C. caspia at 

different chlorine concentrations was dose-dependent. An average decrease of 23% in 

the growth rate was observed at 0.1 mg/L residual chlorine when compared to control 

experiments, over a period of 7 d. No growth was recorded at 1 mg/L residual chlorine, 

indicating threshold levels of residual chlorine on C. caspia. The size (length and width) 

of hydranths decreased significantly as the chlorine concentration increased. Complete 

hydranth degeneration in 3 d was observed at 1 mg/L residual chlorine. The results also 

showed that temperatures >30°C (but not lower temperatures) hastened mortality due to 

chlorine. The combined use of chlorination (0.2 mg/L residual chlorine) and heat (30°C) 

decreased the growth rate of C. caspia by more than 59% when compared to 0.2 mg/L at 

20°C. 

Rajagopal, S., M. Van Der Gaag, G. Van Der Velde and H.A. Jenner. 2002d. Control of 

brackish water fouling mussel, Mytilopsis leucophaeata (Conrad), with sodium 

hypochlorite. Archives of Environmental Contamination and Toxicology 43(3): 296-300. 

 Though the Conrad's false mussel, Mytilopsis leucophaeata, is an important 

fouling animal in industrial cooling water systems, there are no published reports on the 

tolerance of this species to chlorination. A series of experiments was conducted to 

determine the effects of mussel size (2-20 mm shell length), season (breeding versus 

nonbreeding), nutritional status (fed versus starved) and acclimation temperature (5-

30°C) on the mortality pattern of M. leucophaeata under continuous chlorination (0.25-5 

mg/L). The effect of mussel size on M. leucophaeata mortality in the presence of 

chlorine was significant, with 10 mm size group mussels showing greater resistance. At 

0.25 mg/L residual chlorine, 2 mm size group mussels took 89 days to reach 100% 

mortality, whereas 10 mm size group mussels took 109 days. M. leucophaeata collected 

during nonbreeding season (December-April) was more tolerant to chlorine than those 

collected during breeding season (June-October). Nutritional status of the mussel had no 

significant influence on the chlorine tolerance of the mussel: fed and starved mussels 

succumbed to chlorine at equal rates. The effect of acclimation temperature on M. 

leucophaeata mortality in the presence of chlorine was significant. At 0.5 mg/L residual 

chlorine, mussels acclimated at 5°C required 99 days to reach 95% mortality, whereas 

mussels acclimated at 30°C required 47 days. A comparison of present data with 

previous reports suggests that resistance of M. leucophaeata to chlorination is higher than 

other mussel species causing fouling problems in The Netherlands (Mytilus edulis and 

Dreissena polymorpha). 

67 
Rajagopal, S., G. Van Der Velde, M. Van Der Gaag and H.A. Jenner. 2003a. How 

effective is intermittent chlorination to control adult mussel fouling in cooling water 

systems? Water Research 37(2): 329-338. 

 Mussel control in cooling water systems is generally achieved by means of 

chlorination. Chlorine is applied continuously or intermittently, depending on cost and 

discharge criteria. In this paper, we examined whether mussels will be able to survive 

intermittent chlorination because of their ability to close their valves during periods of 

chlorination. Experiments were carried out using three common species of mussels: a 

freshwater mussel, Dreissena polymorpha, a brackish water mussel, Mytilopsis 

leucophaeata and a marine mussel, Mytilus edulis. The mussels were subjected to 

continuous or intermittent (4 h chlorination followed by 4 h no chlorination) chlorination 

at concentrations varying from 1 to 3 mg/L and their responses (lethal and sublethal) 

were compared to those of control mussels. In addition, shell valve activity of mussels 

was monitored using a Mussel-monitor(R). Data clearly indicate that mussels shut their 

valves as soon as chlorine is detected in the environment and open only after chlorine 

dosing is stopped. However, under continuous chlorination mussels are constrained to 

keep the shell valves shut continuously. The mussels subjected to continuous 

chlorination at 1 mg/L showed 100% mortality after 588 h (D. polymorpha), 966 h 

(Mytilus edulis) and 1104 h (Mytilopsis leucophaeata), while those subjected to 

intermittent chlorination at 1 mg/L showed very little or no mortality during the same 

periods. Filtration rate, foot activity index and shell valve movement of D. polymorpha, 

Mytilopsis leucophaeata and Mytilus edulis decreased more than 90% at 1 mg/L chlorine 

residual when compared to control. However, mussels subjected to intermittent 

chlorination showed a similar reduction (about 90%) in filtration rate, foot activity index 

and shell valve movement during chlorination and 3% during breaks in chlorination. The 

data indicate that intermittent chlorination between 1 and 3 mg/L applied at 4 h on and 4 

h off cycle is unlikely to control biofouling if mussels are the dominant fouling 

organisms. 

Rajagopal, S., V.P. Venugopalan, G. Van Der Velde and H.A. Jenner. 2003b. 

Comparative chlorine and temperature tolerance of the oyster Crassostrea madrasensis: 

Implications for cooling system fouling. Biofouling 19(2): 115-124. 

Crassostrea madrasensis is an important fouling oyster in tropical industrial 

cooling water systems. C. madrasensis individuals attach to surfaces by cementing one 

of their two valves to the substratum. Therefore, oyster fouling creates more problems 

than mussel fouling in the cooling conduits of power stations, because unlike the latter, 

the shell of the former remains attached to the substratum even after the death of the 

animal. However, there are no published reports on the tolerance of this species to 

chlorination and heat treatment. The mortality pattern and physiological behavior 

(oxygen consumption and filtration rate) of three size groups (13 mm, 44 mm and 64 mm 

mean shell length) of C. madrasensis were studied at different residual chlorine 

concentrations (0.25, 0.5, 0.75, 1, 2, 3 to 5 mg/L) and temperatures (30°C to 45°C). 

68 
Rajagopal, S., V.P. Venugopalan, G. Van Der Velde and H.A. Jenner. 2003c. Tolerance 

of five species of tropical marine mussels to continuous chlorination. Marine 

Environmental Research 55(4): 277-291. 

 The paper examines the relative lethal and sublethal response of five important 

tropical marine mussels (Perna viridis, Perna perna, Brachidontes striatulus, 

Brachidontes variabilis and Modiolus philippinarum) to different chlorine concentrations 

varying from 0.25 to 15 mg/L. The mussels were observed to co-exist in the cooling 

water circuits of a coastal power station that adopted intermittent chlorination as a fouling 

control technique. The five mussel species showed, in response to chlorination, 100% 

mortality at significantly different exposure times, indicating significant species-specific 

variability in chlorine tolerance. For example, at 1 mg/L residual chlorine, B. variabilis

and P. viridis took 288 and 816 h, respectively, to achieve 100% mortality. The time 

taken for 100% mortality decreased with increasing chlorine residual concentration. The 

effect of mussel size (= mussel age) of P. viridis, P. perna, B. striatulus and M. 

philippinarum on mortality was significant between 1 and 5 mg/L residual chlorine, with 

larger mussels showing greater resistance than smaller ones. All mussel species showed 

progressive reduction in physiological activities when chlorine residuals were increased 

from 0 to 1 mg/L. However, species-specific differences in the relative rate of 

physiological activities were observed. Accordingly, relative reduction in physiological 

activities in response to chlorination was the lowest in P. viridis and the highest in B. 

variabilis. The data clearly indicate significant differences in the lethal and sublethal 

responses of the five mussel species to chlorination. The results, therefore, suggest that 

for effective fouling control, chlorine treatment against mussels has to be employed 

judiciously, depending on the mussel species involved. 

Rajagopal, S., V.P. Venugopalan, G. Van Der Velde and H.A. Jenner. 2005. Response 

of mussel Brachidontes variabilis to chlorination. Chemistry & Ecology 21(2):119-132. 

Brachidontes variabilis is a common fouling mussel species in cooling water 

systems of tropical coastal power stations. However, there are hardly any data available 

on the response of B. variabilis to chlorine, a commonly used antifouling biocide. 

Therefore, lethal and sublethal responses of this mussel to chlorine are of considerable 

interest to the industry. The response of mussels in terms of mortality pattern (LT 50 and 

LT 100 ) and physiological activities (oxygen consumption, filtration rate, foot activity 

and byssus thread production) in different size groups (with shell lengths of 7â€“24 mm) 

of B. variabilis was studied in the laboratory under different chlorine concentrations 

(0.25, 0.50, 0.75 and 1.00 mg/L for sublethal responses and 1, 2, 3 and 5 mg/L for 

mortality). The results showed that the exposure time for 100% mortality of mussels 

decreased significantly with increasing chlorine concentration. However, mussel size 

was not a determinant of its chlorine tolerance: all size groups tested (with shell lengths 

of 7.24 mm) took comparable exposure times to reach 100% mortality at a given chlorine 

concentration (1.5 mg/L). All size groups of B. variabilis showed a progressive reduction 

in physiological activities such as oxygen consumption, filtration rate, foot activity and 

byssus thread production, when chlorine residuals were increased from 0 to 1 mg/L. The 

data generated in the present work are compared with similar data available for other 

tropical fouling mussel species to see how far relative chlorine toxicity could have 

69 
influenced the relative distribution of the mussels inside the seawater intake tunnel of a 

power station at Kalpakkam in India. It is shown that in this insufficiently chlorinated 

system, the relative distribution of Brachidontes striatulus, B. variabilis and Modiolus 

philippinarum reflects the relative tolerance of the species to chlorine. 

Rav-Acha, C., H. Shuval and E. Avisar. 1991. Formation of organochlorine compounds 

during chlorination of seawater in power plant cooling systems: A mutagenic assessment. 

MAP Technical Reports Series. FAO/UNEP/IAEA Consult. Meet. on the Accumulation 

and Transformation of Chemical Contaminants by Biotic and Abiotic Processes in the 

Marine Environment, La Spezia, Italy, 24-28 September 1990: No pagination. 

 Samples of Mediterranean Sea water taken at Tel Aviv (Israel) were chlorinated 

in the laboratory at various chlorine concentrations. Samples of power plant cooling sea 

water from the Reading power station were also collected. The organic components of 

these samples were isolated and concentrated using an Amberlite XAD-2/XAD-8 

column, eluted with 95% ethanol. The ethanol extracts were assayed with the four Ames 

Salmonella typhimurium strains TA 98, TA 100, TA 102 and TA 104. The mutagenicity 

was correlated with the formation of volatile and non-volatile organochlorine 

compounds, as determined from gas chromatography (GC)) and Total Organic Halogens 

(TOX) analysis, respectively. All the tester strains gave positive dose-response curves 

with chlorinated sea water, with greater numbers of revertants in larger extract volumes. 

Statistically significant mutagenicity was found with all four strains in seawater 

chlorinated with 10 mg/L chlorine in equivalents of 100-250 ml water. However, 

seawater chlorinated with 2 mg/L chlorine only showed significant mutagenicity with the 

TA 98 and TA 104 strains. Chlorinated sea water discharged from the Reading power 

station only exhibited mutagenicity with strains TA 98 and TA 102, and not with TA 100 

and TA 104. 

Rhoderick, J.C., R.M Block, W.H. Roosenburg and R. Drobeck. 1977. Effects of 

chlorination to the American oyster, Crassostrea virginica at two temperatures. Research 

Association Southeastern Biology Bulletin 24: 80. 

 No abstract. 

Richkus, W.A. and A.J. Lippson. 1975. An evaluation of the impact of the Morgantown 

Thermal Power Plant on macroplankton populations. PB-277082. M.M. Corp. 

Baltimore, MD (USA). Environmental Technology Center Report: 52 pp. 

 Studies of the effect of entrainment on macroplankton at the PEPCO Morgantown 

Steam Electric Station were conducted by the Chesapeake Biological Laboratory in 1972 

and 1973. Densities of fish larvae declined significantly as a result of mechanical and 

thermal damage sustained upon passage through the cooling system. Amphipod and 

opossum shrimp appeared to suffer minimal entrainment damage. Chlorination increased 

ichthyoplankton mortality. 

70 
Roberts, M.H., Jr. 1978. Effects of chlorinated sea water on decapod crustaceans. Pp. 

329-339. In: Jolley, R.L., H. Gorchev and D.H. Hamilton (eds). Water Chlorination 

Environmental Impact and Health Effects, Vol. 2. Ann Arbor Science Publishers, Inc., 

Ann Arbor, MI. 

 No abstract. 

Roberts, M.H., Jr. and R.A. Gleeson. 1978. Acute toxicity of bromochlorinated seawater 

to selected estuarine species with a comparison to chlorinated seawater toxicity. Marine 

Environmental Science 1: 19-30. 

 No abstract. 

Roberts, M.H., Jr., R. Diaz, M.E. Bender and R. Huggett. 1975. Acute toxicity of 

chlorine to selected estuarine species. Journal Fisheries Research Board Canada 32: 

2525-2528. 

 No abstract. 

Roberts, M.H., Jr., C.E. Laird and J.E. Illowsky. 1979. Effects of chlorinated seawater 

on decopod crustaceans and mulinia larvae. U.S. Environmental Protection Agency, 

EPA-600/3-79-031. 

 No abstract. 

Roesijadi, G., D.M. Jacobsen, J.R. Bridge and E.A. Crecelius. 1979. Disruption of 

magnesium regulation in the crab Cancer productus exposed to chlorinated seawater. 

Marine Environmental Research 2(1): 71-84. 

 Exposure of the crab C. productus to chlorinated seawater resulted in alterations 

in hemolymph Na and Mg concentrations. At the highest chlorination levels, which 

approached the 96 h LC50, regulation of both ions was essentially abolished. Reciprocal 

changes in the Na and Mg levels suggested an effect on the activity of the bladder wall, 

which was implicated in the regulation of hemolymph Mg in crustaceans. Exposure to 

1.19 μg/ml applied Cl (0.68 μg/ml TRO) for 96 h resulted in a 4-fold increase in the NH3 

excretion rate. Crabs also contributed considerable Cl demand to the exposure seawater. 

Measurements of TRO in the inhalant seawater and water exiting the crabs' branchial 

chambers indicated a reduction in TRO equivalent to 57% of that initially present in the 

inhalant water (0.51 μg/ml TRO). Measurements of NH3 concentrations in the exposure 

water indicated that NH3 in seawater was consumed in reactions with oxidants. This was 

especially evident at higher levels of chlorination. At 0.58 μg/ml applied Cl and above, 

chlorination resulted in near disappearance of NH3 from the water. 

Roosenburg, W.H., R.M. Block and J.C. Rhoderick. 1977. The influence of chlorine 

produced oxidants on larval stages of the soft clam Mya arenaria. Association 

Southeastern Biology Bulletin 24: 82. 

 No abstract. 

71 
Rosales Casian, J.A. 1991. Seawater chlorination and the survival and growth of early 

life stages of northern anchovy (Engraulis mordax Girard), with reference to power plant 

cooling systems. Ciencias marinas. Ensenada 17(1): 99-117. 

 The viable hatch, survival, growth and lethal concentrations (LC50) for early life 

stages of northern anchovy (Engraulis mordax ) were examined after seawater 

chlorination. 36-hour eggs, and 1-day (yolk-sac), 3 day (yolk-sac absorption) and 5-dayold larvae were exposed to replicated concentrations of 0.0 (controls), 0.05, 0.1, 0.2, 0.5, 

0.8 and 1.0 mg/L. Egg bioassays were of 24-hr duration only in static technique, and up 

to 12 days after hatching for larval series in semistatic technique. Water temperature was 

18°C in all bioassays. The viable hatch of E. mordax eggs was reduced to 47.5% with 

0.1 mg/L. A slight tolerance to chlorination with increased larval age was observed. 

Because of the high and fast mortality in greater concentrations than 0.5 mg/L, only 

growth data in less of 0.2 g/L were obtained. An exception was shown with 5-day larval 

series: survival in only one replicated treatment of 0.8 mg/L reached the twelfth day and 

permitted growth measuring; the mean standard length (6.04 plus or minus 1.24 mm) was 

smaller than that found in controls, the comparison of mean standard lengths in this series 

was significant, growth rates in controls were 0.44 and 0.39 mm/day, and with 0.8 mg/L 

growth rate was 0.26 mm/day. The 24 hLC50 indicated that the egg was the most 

sensitive stage with 0.114/mg/L. For 1-day larval series, the 24 hLC50’s were 0.248 (no 

food) and 0.18 mg/L (Tetraselmis ). For 3-day larvae, 24-hr LC50 was 0.236; 5-day 

larvae showed a greater value (0.799 mg/L), but at 96 hr it decreased to 0.29 mg/L. 

Rosales Casian, J.A., I. Alfonso Hernandez and M. Gregory Hammann. 1990. Effect of 

seawater chlorination on the survival and growth of grunion (Leuresthes tenuis Ayres). 

En Condiciones de Laboratorio. Ciencias Marinas 16(2): 31-46. 

 21 bioassays were done to determine the chlorine effect on the survival and 

growth of 1, 4 and 16 day old Leuresthes tenuis larvae. In the laboratory, larvae were 

maintained under semistatic conditions at 17.8 degree, and fed with Artemia nauplii. 

After chlorination there was a decrease in survival with 0.2 mg/L Cl2 and was null in less 

than two hours with 1.0 mg/L. The lethal concentrations LC50 after the first two hours for 

1, 4 and 16 day larvae were 0.255, 0.15 and 0.119 mg/L Cl2 respectively; the LC50 values 

at 24 h and 48 h were similar. These LC50’s were low considering the dosing levels of 

chlorine that was added to water of power plant cooling systems. Growth data were 

obtained for low chlorine concentration only.(BDO) 

Sanders, J.G. 1982. The effect of water chlorination on the toxicity of copper to 

phytoplankton. University of Maryland, Solomons, MD (USA) Report: No pagination. 

 The application of chlorine to estuarine waters usually results in oxidation of a 

finite quantity of organic carbon, averaging 35 micrometers. The oxidation may be 

salinity dependent; with one exception, all samples of > 5ppt. salinity showed a loss of 

carbon. The quantity of carbon oxidized is independent of the concentration of chlorine 

added, suggesting that only some compounds are attacked and oxidized. In every case, 

72 
the quantity of carbon associated with the smallest molecular weight fraction increased 

after chlorination. Copper distributions within various size fractions of natural organics 

did not change significantly after chlorination. The quantity of carbon oxidized is small 

in comparison to the total compellation capacity of estuarine waters. 

Sanders, J.G. and J. Ryther. 1980. Impact of chlorine on the species composition of 

marine phytoplankton. Water Chlorination: Environmental Impact and Health Effects 

Vol. 3.; 3rd Conference. Colorado Springs, CO.: 631-640. 

 No abstract. 

Sansone, F.J. and T.J. Kearney. 1981. Unusual chlorine kinetics of tropical seawater, 

and the potential environmental effects. PACON Conference: Pacific Congress on 

Marine Technology, Honolulu, HI (USA), 24-27 Apr: No pagination. 

 The kinetics of free halogen disappearance and end-product production following 

the chlorination of oligotrophic tropical seawater are more than a hundredfold slower 

than those reported for temperate coastal seawaters. Tropical seawater heat exchanger 

systems, such as in OTEC plants, thus require less chlorine for biofouling control than do 

comparable mainland facilities; this environmental advantage, however, is likely to be 

negated by the much longer residence time of the toxic free halogen. Caution is thus 

advised in the use of data from temperate waters to predict environmental chemical 

reactions and effects in tropical systems. 

Santos, C. 1985. Zooplankton entrainment evaluation in a power plant station. Fourth 

Symposium on Coastal and Ocean Management -- Coastal Zone '85, 30 Jul-2 Aug 1985. 

(World Meeting Number 853 0607), Baltimore, MD (USA): No pagination. 

 No abstract. 

Saragolia, M.G. and G. Scarano. 1979. Influences of molting on the sensitivity of toxics 

to the crustacean Penaeua kerathurus. Ecotoxicol Environmental Safety 3: 310-320. 

 No abstract. 

Saravanane, N., K.K. Satpathy, K.V.K. Nair and G. Duraira. 1998. Preliminary 

observations on the recovery of tropical phytoplankton after entrainment. Journal of 

Thermal Biology 23(2): 91-97. 

 Chlorination is practiced for preventing biofouling in the cooling water systems of 

Madras Atomic Power Station. Results of laboratory experiments on phytoplankton 

recovery (as measured by absorbance and cell count) showed that the recovery time in 

process sea water effluents and condenser sea water effluents occurred after 3 and 5 days, 

relative to the natural intake water. Results also showed increasing dominance of a single 

phytoplankton species (Thalassiosira sp.) in the condenser as well as process water 

during the post recovery period. Significant differences in absorbance, gross primary 

73 
productivity and total respiration rate were observed between intake water versus 

condenser water and intake water versus process water. 

Scott, G.I. and D.P. Middaugh. 1978. Seasonal chronic toxicity of chlorination to the 

American oyster, Crassostrea virginica G. Pp. 311-328. In: Jolley, R.L., H. Gorchev 

and D.H. Hamilton (eds). Water Chlorination Environmental Impact and Health Effects 

Vol. 2 Ann Arbor Science Publishers, Inc., Ann Arbor, MI. 

 No abstract. 

Scott, G.I., W.P. Davis, J.M. Marcus, T.G. Ballous and J.A. Dahlin. 1989. Acute 

toxicity, sublethal effects and bioconcentration of chlorination products, viruses, and 

bacteria in edible shellfish: A review. Grant EPA-R-813138. Prepared in cooperation 

with Research Planning Inst., Inc., Columbia, SC. Sponsored by Environmental 

Research Lab., Gulf Breeze, FL (USA). 

 The report identifies, synthesizes, and summarizes published scientific data 

concerning toxicity, sublethal physiological effects, and uptake/depuration rates of 

chlorine, viruses, and bacteria in edible marine shellfish of the United States. The 

summary may provide environmental managers with information related to coastal zone 

issues, such as point-source pollution permits, hazardous materials, material spills, and 

non-point-source runoff regulations. 

Seegert, G.L. and A.S. Brooks. 1978. The effect of intermittent chlorination on coho 

salmon, alewife, spottail shiner, and rainbow smelt. Transactions of the American 

Fisheries Society 107(2): 346-353. 

 Coho salmon (Oncorhynchus kisutch), alewife (Alosa pseudoharengus), spottail 

shiner (Notropis hudsonius) and rainbow smelt (Osmerus mordax) were exposed for 30 

minutes to residual chlorine. The alewives were tested at 10, 15, 20, 25 and 30°C, the 

salmon and shiners at 10, 15 and 20°C and the smelt at 10°C. Thirty-minute LC50 values 

were both species- and temperature-dependent. All species showed an inverse 

relationship between test temperatures and LC50 values. The range of LC50 values 

observed, with the test temperatures in parentheses, were coho salmon, 0.29 (20°C)-0.56 

mg/L (10°C); alewife, 0.30 (30°C)-2.27 mg/L (15°C); spottail shiner, 0.53 (20°C)-2.41 

mg/L (10°C); and smelt, 1.27 mg/L (10°C). Mortalities usually occurred within 24 h 

after exposure. Except for the 10°C spottail shiners, fish rarely recovered following loss 

of equilibrium. Concentrations resulting in no mortality were approximately 0.6-0.7 of 

the LC50 value. 

Sheridan, P.F. and A.C. Badger. 1981. Response of experimental estuarine communities 

to continuous chlorination. Estuarine Coastal and Shelf Science 13(3): 337-347. 

 The effects of continuous chlorination (as NaOCl) on estuarine benthic organisms 

were investigated with plankton-derived experimental communities. Twelve consecutive 

studies were conducted, each of which consisted of approximately 60-day colonization 

74 
periods from flowing estuarine waters that continuously received nominal concentrations 

of 0.00, 0.47, 0.94 or 1.41 mg Cl-produced oxidant CPO) 1-1. Significant chlorination 

effects on numerical abundances of 8 dominant species were detected in data pooled over 

the 12 consecutive studies, although only 2 spp. were significantly affected in 

proportional abundance. There was also a significant decline in total numbers of 

individuals collected in chlorinated communities. Significant differences in short-term 

community structures were found in only 4 of 12 experiments and were related to 

changes in species dominance and increased toxicity due to extreme cold weather. 

 

Sikka, H.C., K.C. Foote, J.I. Manji and E.J. Pack. 1979. Effects and fate of sewage 

chlorination products in phytoplankton. N.L.S.D. Syracuse Research Corp., Govt 

Reports Announcements. Issue 26: 68 pp. 

 The effects of seven stable chloro-organic compounds formed during chlorination of 

domestic waste-water on the growth of selected fresh-water and marine phytoplankton 

were determined. The uptake and metabolism of selected chloro-organic chemicals by 

the phytoplankton were also investigated. 3-Chlorophenol, 3-chlorobenzoic acid, 4-

chlororesorcinol,5-chlorouracil, 5-chlorouridine, 6-chloroguanine or 8-chlorocaffeine at a 

concentration of 0.1 ppm, alone or in combinations of up to 4 chemicals, had no 

significant effect on the yield of Scenedesmus obliguus, Selenastrum capricornutum, 

Microcystis aeruginosa, Dunaliella tertiolecta, Skeltonema costatum, Thalassiosira 

pseudonana, and Porphyridium sp. 4-Chlororesorcinol and 5-chlorouracil were taken up 

by certain species but neither chemical was accumulated to a high level. The uptake of 

chlororesorcinol was considerably greater than that of chlorouacil. The uptake of 3-

chlorobenzoic acid by the phytoplankton was negligible. 4-Chlororesorcinol was read 

Smith, M.K., H. Zenick and E.L. George. 1986. Reproductive toxicology of disinfection 

by-products. Environmental Health Perspective 69: 177-182. 

 The chronic exposure of large segments of the population to disinfected drinking 

water has necessitated an evaluation of the health effects of the by-products of the 

chlorination process. This paper reviews the available information concerning the 

reproductive consequences associated with exposure to disinfection by-products. Four 

groups of compounds are discussed: the trihalomethanes, in particular chloroform; the 

chlorinated phenols; chlorinated humic substances; and the haloacetonitriles. In the 

pregnant female, chloroform and the 2- and 2,4-chlorophenols produced low levels of 

embryo- and fetotoxicity. Chloroform induced terata when administered by inhalation. 

The chlorinated humic substances and 2,4,6-trichlorophenol were without significant 

reproductive effects. The haloacetonitriles showed in utero toxicity, becoming more 

severe with increasing halogen substitution. 

Sriraman, A.K. and R. Viswanathan. 1983. Effects of chlorinated cooling water 

discharges on the chemistry of estuarine waters. Mahasagar 16(2): 259-263. 

 The increasing tendency towards locating power plants at coastal and estuarine 

sites is in a large measure due to the practically unlimited amounts of cooling water 

75 
available in these regions. This has also stimulated systematic investigations on the 

impact of the discharges on the chemistry of the receiving waters. A case in point is the 

use of chlorine for pretreatment of cooling waters. Available data indicate that chemical 

species which result from chlorination of seawater differ significantly from those of 

freshwater. In the present paper, these differences are discussed in relation to estuaries 

which contain varying proportions of freshwater and seawater. A critical account is 

given of the models proposed to explain the reactions, behavior and persistence of 

chlorine in discharge plumes in estuaries. 

Stanbro, W.D. 1983. Chemistry of power plant chlorination. Report PB-84-127422., 

Applied Physics Lab, Johns Hopkins Univ., Laurel, MD (USA): 32 pp. 

 This report presents a summary of the present knowledge of chlorine chemistry as 

used for biofouling control in the electric utility industry. The level of the presentation is 

appropriate for the non-specialist who must regulate the use of chlorine in power plant 

cooling waters. The areas covered in this report are the practice of chlorination, fresh 

water chlorine chemistry, marine and estuarine water chlorination chemistry, analysis of 

chlorination products, models of chlorination chemistry and chemical dechlorination. 

Stanbro, W.D. and M.J. Lenkevich. 1982. Slowly dechlorinated organic chloramines. 

Science 215(4535): 967-968. 

 Dechlorination of some organic chloramines with aqueous sulfite solutions does 

not take place instantaneously as previously assumed. Field dechlorination times on the 

order of hours for some compounds that are found in chlorinated effluents appear likely 

on the basis of laboratory studies. These chlorinated compounds are not detected by 

standard analytical methods in the presence of sulfite ion. 

Stenton-Dozey, J.M.E. and A.C. Brown. 1994. Exposure of the sandy-beach bivalve 

Donax serra Roeding to a heated and chlorinated effluent. 1. Effects of temperature on 

burrowing and survival. Journal of Shellfish Research 13(2): 443-449. 

 The potential impact of the thermal plume from a nuclear power station on 

burrowing and survival of the bivalve Donax serra, which inhabits a nearby sandy beach, 

was investigated. Median lethal time and median lethal temperature were used to define 

the size-related upper thermal tolerance of D. serra. Small individuals would best 

tolerate heated effluent from the power station. Temperatures above 32°C were lethal to 

all sizes of D. serra. After extended exposure to temperatures between 24 and 29°C, 

50% of the animals no longer remained buried. Because these temperatures occur in the 

thermal plume, such displacement from the sand can result in exposure to predation as 

well as possible stranding on the beach. 

Stewart, M.E., W. Blogoslawski and G.R. Helz. 1979. Byproducts of oxidative biocides 

toxicity to oyster larvae. Marine Pollution Bulletin 10(6): 166-169. 

76 
 Selected by-products which are produced upon chlorination or ozonization of 

seawater were examined for their effect on eastern oyster (Crassostrea virginica) larvae. 

The compounds bromate, bromoform and chloroform were studied at 0.05, 0.1, 1.0 and 

10.0 mg/L. Repeated bioassays indicated that even at these low levels, all 3 substances 

produced some larval mortality. Oxidation by-products formed during chlorination or 

ozonization of power plant cooling waters may have adverse effects on the growth of 

marine invertebrates such as C. virginica during their delicate larval stages. 

Stober, Q.J. and C.H Hanson. 1974. Toxicity of chlorine and heat to pink 

(Oncorhynchus gorbuscha) and chinook salmon (O. tshawytscha). Transnational 

American Fish Society 103: 569-576. 

 No abstract. 

Stober, Q.J., P.A. Dinnel, E.F. Hurlberg, D.H. DiJulio, S.P Felton and R.E. Nakataini. 

1978. Effects of seawater chlorination on marine organisms. Report No. UW-NRC-9. 

Department of Fisheries, University of Washington, Seattle, WA. 

 No abstract. 

Sugam, R. and G. R. Helz. 1981. Chlorine speciation in seawater; a metastable 

equilibrium model for ClI

 and BrI species. Chemosphere 10(1): 41-57. 

 A thermodynamic model for the inorganic, fast-reacting fraction of the total 

residual oxidant produced by chlorination in marine waters reveals that BrI species are 

more stable than ClI

 species. At typical amino-N levels, HOBr, NBr3, and NHBr2

predominate. Data on organic byproducts of chlorination generally support the model 

calculations. 

Sung, R., D. Strehler and C. Thorne. 1978. Assessment of the effects of chlorinated 

seawater from power plants on aquatic organisms. Final task report, May-September 

1978. Technical Report. T.E.E. Div. Redondo Beach, CA (USA). 73 pp. 

 The report gives a detailed review of past and present research efforts on the 

effects of chlorinated seawater from power plants on aquatic organisms. It includes: (1) 

A characterization of chemical species contained in power plant seawater discharges; (2) 

a review of the amperometric titration method for residual chlorine determinations in 

seawater; and (3) an analysis of the toxicity of compounds formed by chlorination of 

seawater. The review concluded that (1) the toxicity of chlorinated seawater effluent is 

due primarily to various oxidant residuals produced by chlorination, rather than to 

residual chlorine itself; (2) the amperometric titration method is adequate to determine 

safe oxidant levels when identification of specific compounds is not required; (3) 

bromoform is a principal contributor to toxicity in power plant discharges (because of 

volatility and degradability, bromoform is not expected to be as toxic as chloroform); and 

(4) other compounds suspected of causing toxicity have not been clearly identified. 

77 
Tachikawa, M., T. Aburada, M. Tezuka and R. Sawamura. 2005. Occurrence and 

production of chloramines in the chlorination of creatinine in aqueous solution. Water 

Research 39(2-3): 371-379. 

 Occurrence and production of stable chloramines in the chlorination of creatinine, 

a constituent of perspiration and urine, in aqueous media were studied. Creatinine 

(5×10−5

 M) was treated with free chlorine in aqueous solutions at molar ratios of 0.5–8 

(chlorine/creatinine) at pH 7.0 at room temperature for several days. At lower ratios of 

chlorine, two stable N-chlorocreatinine derivatives, which were determined as 

dichloramine fractions by the DPD method, were isolated by HPLC and identified by EIMS and 1

H-NMR. One was 2-chloroamino-1-methylimidazolin-4-one (creatinine 

chloramine) and the other was 2-chloroamino-5-hydroxy-1-methylimidazolin-4-one 

(hydroxycreatinine chloramine). In addition, the formation of methylamine was 

identified by GC-MS analyses of its imine derivative formed with 

pentafluorobenzaldehyde. Methylamine forms stable chloramines, which might be 

determined as mono- and/or di-chloramine fractions together with free chlorine by the 

DPD method in the reaction mixtures at higher molar ratios of chlorine. In practice, 

small amounts of methylamine (~19 μg/L) were detected in water samples collected from 

several swimming pools. Hence, methylamine may be an origin of elusive organic 

chloramine formed in the chlorination of swimming pools. A probable mechanism of the 

occurrence and processing of chlorination products of creatinine is suggested. 

Thatcher, T.O. 1977. An effect of chlorination on the hatching of coon stripe shrimp 

eggs: so what. Proceedings National Shellfish Association 67: 71-74. 

 No abstract. 

Thatcher, T.O. 1978. The relative sensitivity of Pacific northwest fishes and 

invertebrates to chlorinated seawater Pp. 341-350. In: Jolley, R.L., H. Gorchev and D.H. 

Hamilton (eds). Water Chlorination Environmental Impact and Health Effects Vol. 2 

Ann Arbor Science Publishers, Inc., Ann Arbor, MI. 

 No abstract. 

Thatcher, T.O. 1979. A morphological defect in shiner perch resulting from chronic 

exposure to chlorinated sea water. Bulletin Environmental Continental American 

Toxicology 21: 433-438. 

 No abstract. 

Thompson, I.S., R. Seed, C.A. Richardson, L. Hui and G. Walker. 1997. Effects of low 

level chlorination on the recruitment, behavior and shell growth of Mytilus edulis

Linnaeus in power station cooling water. Scientia Marina Vol. 61 (SUPPL. 2): 77-85. 

 Chlorination procedures are widely used to control biofouling in the cooling water 

systems of coastal power stations. However, relatively little is known about the ways in 

78 
which these procedures affect the ecology of major fouling organisms such as the mussel, 

Mytilus edulis. High concentrations of chlorine (8 mg/L), as sodium hypochlorite 

(NaOCl), lead to mortality of all planktonic larval stages, while lower concentrations (1 

mg/L), produce changes in the swimming and crawling behavior of larvae in experiments 

conducted in non-flow through systems. The age and growth history of individual 

mussels collected from power station culverts were determined from the growth lines 

present in acetate peels of polished and etched shell sections. During periods of 

chlorination shell growth is severely reduced resulting in marked changes in shell 

structure and deposition. Consequently, growth rates of naturally occurring mussels were 

substantially greater than those occurring within the culverts. The information provided 

by this study, together with an ongoing monitoring programme of the natural periods of 

mussel settlement on artificial substrata, is currently being used to develop an appropriate 

chlorination protocol at Wylfa Nuclear Power Station, North Wales, UK. The use of 

mussels to evaluate the long-term effects of low level chlorination is briefly discussed. 

Turnpenny, A. and C.J.L. Taylor. 2000. An assessment of the effect of the Sizewell 

power stations on fish populations. Hydroecologie Appliquee. 12(1): 87-134. 

 Sizewell A and B Nuclear Power Stations are located on the Suffolk Coast of East 

Anglia. The A station is a 650 MWe Magnox plant, completed in 1966 and operated by 

British Nuclear Fuels; the B station is a 1258 MWe pressurised water reactor (PWR), 

commissioned in 1995 and operated by British Energy Generation Ltd. Both power 

stations are direct cooled and rely on abstractions of cooling water (CW) from the North 

Sea: together they can abstract some 80 m3s

-1. The water is passed around the plant 

condenser circuits and returned to the sea, along with reject heat and any chlorine 

residues from antifouling treatment. The abstraction of this water is accompanied, to 

some extent unavoidably, by entrained fish present either as ichthyoplankton (eggs, 

larvae and postlarvae of fish), or as fully-formed juvenile or adult fish which have to be 

removed by mechanical screening systems ("drum" screens) to avoid CW condenser 

blockage. The entrained ichthyoplankton passes through the entire cooling system and is 

discharged back to sea along with the heated water. The later life stages of fish and other 

material that become impinged upon the drum screens are removed from the water. At 

Sizewell B provision is made to return the more robust species of fish back to the sea 

alive. The results of these studies are frequently presented in this report in terms of 

Equivalent Adult Values (EAV's). The EAV method is a procedure where the numbers 

of fish of any age are standardized to the number that would be expected to be alive at the 

age when 50% of the stock would mature. Thus, if a fish matures at 3 years old, many 

millions of eggs or larvae may represent a single "equivalent adult", and the EAV will be 

a tiny fraction of unity, whereas a fish older than 3 years will have an EAV of greater 

than one. The purpose of the EAV method is to allow fish captured at any stage of their 

life cycle to be compared on an equal footing with fish of commercial size. It is 

important to note that the EAV method does not take account of density-dependent 

factors (such as rates of predation, parasitism, feeding success) that might tend to 

increase the survival, growth and reproductive rates of individuals left in the population 

when some of their competitors are removed. The values given should therefore be 

regarded as overestimates. 

79 
Van Der Velde, G., S. Rajagopal and H.A. Jenner. 1997. Sublethal responses of zebra 

mussel, Dreissena polymorpha (Pallas) to low-level chlorination: A laboratory study. 

Conference Proceedings: Seventh International Zebra Mussel and Aquatic Nuisance 

Species Conference. 

 Sublethal responses (filtration rate, foot activity index, byssus thread production 

and byssus thread strength) of different size groups of zebra mussel, Dreissena 

polymorpha (Pallas) exposed to seven different chlorine concentrations (control, 0.1, 0.2, 

0.3, 0.4, 0.5 and 1.0 mg/L) were investigated in the laboratory. All size groups of 

mussels showed progressive reduction in physiological activities when chlorine 

concentrations were increased from 0 to 1 mg/L. Mussels exposed to greater than or 

equal to 0.1 mg/L showed considerable reduction in physiological activities when 

compared to control experiments (e.g., about 15% reduction in mean filtration rate at 0.1 

mg/L). However, no significant differences in physiological activities were observed 

between 0.5 and 1.0 mg/L chlorine residuals (e.g., 89% and 93% reduction in foot 

activity index at 0.5 and 1.0 mg/L, respectively). The reduction in physiological 

activities of D. polymorpha was strongly correlated with the concentration of chlorine. 

Resistance of D. polymorpha to chlorine appeared to be lower than that found for other 

mussel species in The Netherlands viz., Mytilus edulis L. and Myrtilopsis leucophaeta

(Conrad). 

Vanderhorst, J.R. 1982. Effects of chlorine on marine benthos. Report EPRI-EA-2696. 

Battelle-Northwest Marine Research Lab, Sequim, WA (USA): 102 pp. 

 Studies were conducted to investigate the effects of chlorination on epibenthic 

communities in open microcosms and to evaluate open-microcosm methodology. A 

single experiment provided for two years of exposure to target concentrations of 10 and 

50 ppB of chlorine-produced oxidants (CPO) in sea water. Continuous and intermittent 

chlorination regimes were used at each of the concentrations. The experiment was 

conducted in triplicate and included triplicate controls not receiving chlorination. There 

was an increase in the number of species for communities receiving each of the 

treatments, but there were significant (p = 0.05) differences in the rate of increase 

between intermittent and continuous chlorination regimes and between the two target 

concentrations within each of the regimes. Continuously chlorinated communities 

increased less rapidly in the number of species than did intermittently chlorinated 

communities. Communities receiving 50 ppB CPO increased in the number of species 

less rapidly than did communities receiving 10 ppB CPO (p = 0.05). There were 

significant (p = 0.05) effects on community complexity attributable to the distance 

between microcosms and the central head tank supplying all microcosms. Experimental 

substrates placed closer to the in-flow end of microcosms exhibited more animal species 

and fewer plant species than did experimental substrates placed closer to the out-flow end 

of individual microcosms. 

80 
Videau, C., M. Khalanski and M. Penot. 1979. Preliminary results concerning effects of 

chlorine on mono-specific marine phytoplankton. Journal of Experimental Marine 

Biology and Ecology 36: 111-124.

 Static chlorination tests were conducted on 3 phytoplanktonic marine spp., 

Dunaliella primolecta Butcher, Pavlova lutheri (Droop) Green (= Monochrysis lutheri

Droop) and Phaeodactylum tricornutum Bohlin. The specific response and the sensibility 

threshold of the algae tested: the chlorine LD50 for the least dense culture (103 cells 

.cntdot. ml-1) of Dunaliella and Pavlova is 0.4 and 4.0 ppm, respectively, while the 

growth of Phaeodactylum is reduced or ceases at a chlorine concentration of 0.6 ppm. 

Influence of cellular density: chlorine toxicity increases with decreasing cellular 

concentration (tests from 103-106 cells .cntdot. ml-1). The effect on chlorophyll a 

concentration is not affected when the mortality due to the chlorine injection is < 50%; 

the chlorophyll a concentration is greatly reduced for higher mortality. Influence of light: 

chlorinations conducted in the dark produce a lower mortality, for Dunaliella, than 

chlorinations conducted in the light; the mortality is lower as long as the cultures are kept 

in the dark. Chlorine as a disinfectant is applied at power plant cooling systems on 

estuaries and by the sea. The chlorination has an algicidal effect on fouling organisms 

but there is also a 90% decline in primary production. 

Vreenegoor, S.M., R.M. Block, J.C. Rhoderick and S.R. Gullans. 1977. The effects of 

chlorination on the osmoregulatory ability of the blue crab, Callinectes sapidus. 

Association Southeastern Biology Bulletin 24: 93. 

 No abstract. 

Waugh, G.D. 1964. Observations on the effects of chlorine on the larvae of oysters 

(Ostrea edulis L) and barnacles (Eliminius modestus Darwin). Applied Biology 54: 426-

440. 

 No abstract. 

Water Pollution Control. 1973. What you've always wanted to know about chlorination. 

Water Pollution Control. 111(1): 19-22. 

 The article deals with the types of chlorination used for pollution control, the 

chlorinators available and the control systems used. The primary purpose of chlorination 

is briefly reviewed. The article is intended to bring the knowledge contained in the 

former Ontario Water Resources Commission's manuals [cf. Journal American Water 

Works to Society] to a larger public. 

White, W.R. 1966. The effect of low-level chlorination on mussels at Poole Power 

Station. Report No. RD/L/N17/66. Central Electricity Research Laboratories, 

Leatherhead, England. 

 No abstract. 

81 
White, W.R. 1969. Settlement and growth of mussels. Symposium on Marine Biology, 

Research Lab Memorandum No. RD/L/M 269. Central Electricity Research 

Laboratories, Leatherhead, England. Pp. 39-40. 

 No abstract. 

Wickramanayake, G.B. and A.R. Gavaskar. 2000. Second international conference on 

remediation of chlorinated and recalcitrant compounds. Monterey, CA, May 2000. 

ISBN: 1-57477-094-2. Seven vol. set available for $399.50 from Battelle, 505 King 

Ave., Columbus, OH 43201-2693. 

 No abstract. 

Williams, E.E. and B. Knox-Holmes. 1989. Marine biofouling solutions for closed 

seawater systems. Sea Technology 30(6): 17-18. 

 The patented BFCC antifouling and corrosion protection system--marketed by 

Biofouling and Corrosion Control Ltd., a University of Sheffield-based company that 

specializes in consultative work in this technical area--is intended to control biofouling in 

closed seawater systems on, for example, ships, offshore production platforms, 

desalination plants, and power stations. The antibiofouling efficiency of conventional 

electrolytic chlorine dosing, copper dosing, copper and aluminum dosing (a commercially 

available system), and a combined copper/chlorine BFCC treatment have been compared 

in field trials conducted in conjunction with the CEGB, the U.K. national power utility, 

and in independent tests at the University of Miami, Florida, in association with EPRI 

and Stone and Webster. The copper/chlorine (BFCC) system was found to be the most 

efficient at reducing fouling both inside steel pipes and on titanium heat exchange 

surfaces. Results indicate that the BFCC system is at least as effective as a conventional 

hypochlorite treatment at levels of chlorine between five and ten times less than those 

used in traditional hypochlorite dosing and at copper levels one-sixth of those required to 

kill macro-fouling. 

Wilson, T.C. 1977. The effect of chlorine residuals on the blue rockfish (Sebastes 

mystinus), the rock prickleback (Xyphister mucosus) and the copepod (Calanus 

pacificus). Report No. 7846. 

 No abstract. 

Winklehaus, C. 1977. Chlorination: assessing its impact. Journal Water Pollution 

Control Federation 49(12): 2354-2357. 

 A brief summary is given of the topics of the ``Second Conference on Water 

Chlorination: Environmental Impact and Health Effects`` held jointly by Oak Ridge 

National Laboratory, the U.S. Environmental Protection Agency, and the U.S. 

Department of Energy. Some 65 papers relating to chlorination were heard. The themes 

were: fresh water systems; marine systems; toxicity in aquatic systems; trihalomethanes; 

82 
waste water disinfection; health effects; industrial effluents; alternatives; and regulations, 

progress, and problems. The EPA overview of the regulatory aspects of chlorination was 

presented. 

Woehrling, D. and G. Le Fevre-Lehoerff. 1997. Long-term series in ichthyoplankton 

and related species: Sole and sprat eggs and larvae at the French Coast of the North Sea 

during 20 years Ichthyoplankton Ecology, Fisheries Society of the British Isles 

Conference: No pagination. 

 Monitoring ichthyoplankton and environment at a control point in front of the 

nuclear power plant of Gravelines (French coast of the North Sea Southern Bight) since 

1975 yields long-term series for eggs and larvae of sole (Solea vulgaris Quensel) 

Xavier de Brito, A.P., I.M.R. de Andrade Bruning, I. Moreira and I. Loureiro. 1999. 

Occurrence of chlorinated pesticides in mussels of Guanabara Bay, Rio de Janeiro, 

Brazil. Marine Pollution Bulletin Toxicology In Vitro (Jul): 603-604. 

 Chlorinated pesticides are common Pollutants in the aquatic environment in 

coastal areas and estuaries. Guanabara Bay is a coastal area of great economical 

importance and leisure interest in the Brazilian Southeast Coastal It is surrounded by two 

cities, Rio de Janeiro and Niteroi, with a total population of about eleven million 

inhabitants. The Bay receives a daily discharge of chemical contaminants introduced 

with several tons of domestic, industrial and agricultural treated and non-treated sewage. 

Part of these contaminants may be incorporated by the living organisms of this ecosystem 

and consist of potential danger to human consumption. Present work investigates the 

contamination of some chlorinated pesticides in common mussels (Perna perna), from 

the Guanabara Bay. These mussels can be found in several points near the entrance of 

the Bay, where they either grow naturally or are cultivated by fishermen which make 

their living of this activity. Five collecting areas were studied and the concentrations of 

eight pesticides, namely HCB, gamma -HCH, total DDT, DDD and DDE, Aldrin, 

Dieldrin and Endrin were determined. The samples were collected twice a year, during 

the dry (August) and rainy (December) seasons of 1996. The mussels were manually 

collected, wrapped in aluminium foils previously rinsed with acetone, ethanol and hexane 

for pesticide analysis and kept frozen until reach the laboratory. After selection, 10 (ten) 

mussels of similar size (4-6 cm) composed one sample for each location. The muscle 

tissues were separated from the valves and were cold frozen-dried, so the water content 

was removed without loss of volatile components. The material was ground and 

homogenized and kept ready for the analysis under -10°C. A mussel sample (2g) was 

spiked with known amount of tetrachlorometaxilene (TCMX) and was Soxhlet extracted 

for 24 hours with pesticide grade hexane purified through Florisil columns. 

Yamamoto, K., M. Fukushima and K. Oda. 1990. Effects of stirring on residual chlorine 

during chlorination of seawater containing ammonia nitrogen. Water Research 24(5): 

649-652. 

83 
 Stirring affected the concentration and proportions of residual chlorine during the 

chlorination of seawater containing ammonia nitrogen. At high stirring speed, the 

residual chlorine level rose in direct proportion to the dose of chlorine, then decreased to 

a minimum. At low stirring speed, the residual chlorine level began to decline before the 

peak of the break-point curve, which was flatter than the one obtained with high stirring 

speed. Regardless of the speed of stirring, monochloramine was the predominant species 

in the residual chlorine that remained after 1 h and the break-point was at the same 

chlorine dose. The amount of monobromamine produced was inversely related to the 

speed of stirring. Residual chlorine loss occurred primarily via the disproportionation of 

monobromamine to form dibromamine and ammonia nitrogen. Later, dibromamine and 

bromochloramine disappeared on reacting with monochloramine and only stable 

monochloramine remained. The degree of mixing of chlorine and seawater with 

ammonia nitrogen is of importance in evaluating the residual chlorine concentrations. 

Yang, J.S. 2001. Bromoform in the effluents of a nuclear power plant: A potential tracer 

of coastal water masses. Hydrobiologia 464: 99-105. 

 Bromoform (tribromomethane, CHBr3), one of the trihalomethanes, is a 

chlorination byproduct in cooling water of power plants and industrial complexes. We 

used the distribution of bromoform in seawater to monitor the movement of cooling 

water from the Youngkwang Nuclear Power Plant (YNPP) located on the West Coast of 

Korea. Bromoform concentrations were highest, 124 μg l-1, in surface water near the 

outlet of YNPP and decreased linearly with distance from the outlet, mimicking the 

dissipation of cooling water discharged from the power plant. This byproduct of 

chlorination is thus a potential tracer of coastal water masses, due to its conservative 

behavior in the cooling water, low natural background in seawater, and easy analytical 

detection. 

Yang, Y.-F., Z.-D. Wang, M.-X. Pan and N.-Z. Jiao. 2002. Zooplankton community 

structure of the sea surface microlayer near nuclear power plants and marine fish culture 

zones in Daya Bay. Chinese Journal of Oceanology and Limnology 20(2): 129-134.

 The authors' surveys in May-June 1999 (two cruises) at six sampling 

stations near nuclear power plants (NPP) and marine fish culture zones in Daya Bay, 

Guangdong, revealed species composition, densities and body-size of the sea surface 

microlayer (SM) zooplankton (> 35 μm). Results showed that protozoans and copepod 

nauplii were the predominant components, accounting for 65.40% to 95.56% of total 

zooplankton in abundance. The size-frequency distributions showed that the frequency 

of micro-zooplankton (0.02-0.2 mm) reached 0.8235. The SM zooplankton community 

structure revealed in the present study was quite different from that revealed by 

investigations in the 1980s in Daya Bay. Difference of sampling method has important 

influence on the obtained zooplankton community structure. SM zooplankton consisted 

of micro- and mesozooplankton (0.2-2.0 mm), with micro-zooplankton being 

predominant. Some possible cause-effect relations between the zooplankton community 

structure and mariculture, nuclear power plants cooling systems and sampling method are 

discussed. 

84 
Yosha, S.F. and G.M. Cohen. 1979. Effect of intermittent chlorination on developing 

zebrafish embryos (Brachydanio rerio). Bulletin of Environmental Contamination and 

Toxicology 21(4-5): 703-710. 

 The primary objective was to determine whether chlorine adversely affects the 

hatchability of freshwater fish embryos and to what extent, if any, chlorine toxicity is 

modified by the ionic composition of the water. Contrary to initial expectations , 

chlorine, even at a relatively high concentration (1 mg/mL total residual chlorine), was 

not toxic to developing zebrafish embryos prior to hatching. However, chlorine in the 

presence of a mild external ionic stress (CaCl2 and MgCl2) significantly reduced the 

number of embryos hatched. Chlorine was toxic to newly hatched larvae in all solutions 

tested, whereas no mortalities of the newly hatched larvae occurred in any of the 

solutions in the absence of chlorine. Once again ionic stress potentiated the effects of 

chlorine. 

Zeitoun, I.H. and J.Z. Reynolds. 1978. Power plant chlorination. Environmental 

Science and Technology 12(7): 780-784. 

 Cl is used to control slime in power plant cooling waters. Since Cl is toxic, its use 

is regulated. Ways of increasing the effectiveness of rules governing the use of Cl are 

discussed. 

Zhang, X. and R.A. Minear. 2002. Characterization of high molecular weight 

disinfection byproducts resulting from chlorination of aquatic humic substances. 

Environmental Science & Technology 36(19): 4033-4038. 

 Aquatic humic substances react with chlorine to produce numerous disinfection 

byproducts (DBPs) during chlorination of drinking water. Although low molecular 

weight (MW) chlorinated DBPs have been intensively studied over the past several 

decades, relatively little is known about high MW chlorinated DBPs (above 500 Da) that 

may be associated with adverse health implications. In this work, carrier-free radioactive 

36Cl was introduced into a Suwannee River fulvic acid sample to label the chlorinecontaining DBPs. By combining the fractionation techniques of ultrafiltration (UF) and 

size exclusion chromatography (SEC) with the detection of 36Cl, UV, and dissolved 

organic carbon (DOC), the high MW region in the SEC-36Cl profiles of the chlorinated 

sample with and without UF was defined. SEC-UV and SEC-DOC profiles were found 

to be approximately indicative of SEC-36Cl profiles for the high MW region. The MW 

distribution shows that the high MW chlorinated DBPs were highly dispersed with an 

average MW around 2000 Da based on calibration with polystyrene sulfonate standards. 

The Cl/C atomic ratios of the high MW DBPs were roughly constant (0.025), which is 

much lower than those of the common known chlorinated DBPs. 

Zhang, X.M. and R.A. Minear. 2006. Formation, adsorption and separation of high 

molecular weight disinfection byproducts resulting from chlorination of aquatic humic 

substances. Water Research Vol. 40(2): 221-230. 

85 
 A significant portion of the unidentified disinfection byproducts (DBPs) in 

chlorinated drinking water can be attributed to high molecular weight (MW)-chlorinated 

DBPs (above 500Da) that may have adverse health effects. In this work, issues on the 

formation, adsorption and separation of high MW-chlorinated DBPs were investigated by 

introducing radioactive 36Cl into humic substance samples. The results show that the 

amount of high MW-chlorinated DBPs during chlorination decreased with the increase of 

contact time from 1 to 120h, increased with the increase of pH from 5.5 to 9.5, and was 

less in the ultrafiltered samples from Suwannee River fulvic acid than from Suwannee 

River humic acid. The high MW-chlorinated DBPs were found to be effectively 

adsorbed by activated carbon and be possibly reduced to xCl- by activated carbon, but not 

to be readily desorbed from the activated carbon. Those high MW-chlorinated DBPs 

were demonstrated to be incapable of resolution into discrete peaks by any of the three 

liquid chromatography columns studied. The significant implications of these results are 

discussed. 

86 
SUBJECT INDEX

Algae 

Pacheco et al. (1997) 

Bacteria 

Azanza et al. (2001) 

Blatchley (1995) 

Blatchley and Xie (1995) 

Bolster et al. (2005) 

Browdy et al. (1998) 

Choi et al. (2002) 

Ferro-Soto (2001) 

Hamilton et al. (1970) 

Huang et al. (1997) 

Iyer and Varma (1994) 

Junli et al. (1994)

Lovell (1999) 

Benthic invertebrate

Karande et al (1982) 

Oceanographique (1998) 

Sheridan and Badger (1981) 

Stober et al. (1978) 

Vanderhorst (1982) 

Bivalves 

Anderson et al. (1978) 

Baier et al. (1994) 

Beauchamp (1969) 

Behrens and Larsson (1976) 

Bidwell (1999) 

Block (1977) 

Bongers et al. (1977) 

Brungs (1973) 

Domart-Coulon (2000) 

Galtsoff (1946) 

Gibson et al. (1981) 

Goldman (1978) 

87 
Holmes (1969) 

James (1966) 

Karande et al. (1982) 

Ketelaars and Wagenvoort (1995) 

Kim and Lee (1989) 

Lou et al. (1981) 

Marine Research Inc. (1976) 

Masilamoni et al. (2002) 

Meldrim et al. (1974) 

Rajagopal et al. (1997a and b) 

Rajagopal et al. (2002a, b and d) 

Rajagopal et al. (2003a and b) 

Rajagopal et al. (2005) 

Rhoderick et al. (1977) 

Roberts and Gleeson (1978) 

Roberts et al. (1979) 

Roosenburg et al. (1977) 

Scott and Middaugh (1978) 

Scott et al. (1989) 

Stenton-Dozey and Brown (1994) 

Stewart et al. (1979) 

Thompson et al. (1997) 

Xavier De Brito et al. (1999) 

Waugh (1964) 

White (1966; 1969) 

Cellular 

Domart-Coulon et al. (2000) 

Morris et al. (1983) 

Chemical Speciation 

Abarnou and Miossec (1992)

Allonier and Khalanski (1998)

Allonier et al. (1999) 

Amy et al. (1998) 

Anderson et al. (1978; 1979) 

Benanou et al. (1998) 

Burton and Fisher (2001) 

Carpenter et al. (1981) 

Fayad and Iqbal (1987) 

Gibson et al. (1981) 

Graslund et al. (1999) 

88 
Haag (1981) 

Harwood et al. (1989) 

Hall et al. (1981) 

Hergott (1977) 

Hergott et al. (1978) 

Jenner et al. (1997) 

Johnson and Qualls (1983) 

Johnson et al. (1982) 

Jolley (1984) 

Khalanski and Delesmont (1994) 

Kristiansen et al. (1994) 

Kurelec et al. (1983) 

Macalady et al. (1977) 

Middaugh and Davis (1975) 

Mount and Quast (1993) 

Opresko (1982; 1983) 

Peres (1988) 

Rav-Acha et al. (1991) 

Sanders (1982) 

Sansone and Kearney (1981) 

Smith et al. (1986) 

Sriraman and Viswanathan (1983) 

Sugam and Helz (1981) 

Sung et al. (1978) 

Stanbro and Lenkevich (1982) 

Stanbro (1983) 

Stewart et al. (1979) 

Wat. Pollution Control. (1973) 

Williams and Knox-Holmes (1989) 

Yamamoto et al. (1990) 

Yang (2001) 

Zang and Minear (2002) 

Zeitoun and Reynolds (1978) 

Coral 

Oceanographique (1998) 

Decapods 

Bamber and Seaby (2004) 

Behrens and Larsson (1976) 

Block (1977) 

Browdy et al. (1998) 

89 
Capuzzo et al. (1976b; 1977) 

Enstrom and Kirkwood (1974) 

Gibson et al. (1976; 1981) 

Goldman (1978) 

Graslund et al. (1999) 

Hall et al. (1979a and c) 

Public Service Electric and Gas Company (1978) 

Meldrim et al. (1974) 

Mount and Quast (1993)

Richkus and Lippson (1975) 

Roberts (1978) 

Roberts et al. (1979) 

Roesijadi et al. (1979) 

Saraglia and Scarano (1979) 

Thatcher (1977; 1978) 

Vreenegoor et al. (1977) 

Fish (Adult) 

Alderson (1969; 1972; 1974) 

Anderson et al. (1975; 1978) 

Bellanca and Bailey (1977) 

Bender et al. (1977) 

Block (1977) 

Block et al. (1977; 1978) 

Bongers et al. (1977) 

Brungs (1973) 

Buckley (1976; 1977) 

Buckley and Matsuda (1972; 1973) 

Buckley et al. (1976) 

Burton et al. (1979a and b) 

Cakiroglu and Yurteri (1998) 

Capuzzo et al. (1977a and b) 

Consolidated Edison Co. (1973a and b) 

Cooke and Schreer (2001) 

Cripe (1979) 

Enstrom and Kirkwood (1974) 

Gibson et al. (1981) 

Gift’s Bioassay Work (1973) 

Goldman et al. (1978) 

Gullens et al. (1977) 

Hall et al. (1979) 

Holland et al. (1960) 

Hose et al. (1989) 

Hoss et al. (1975; 1977) 

90 
Hughes (1972; 1975) 

Johnson et al. (1977) 

Laurer’s Bioassay Work (1973) 

Liden and Burton (1977) 

Liden et al. (1980) 

Marcy (1973) 

Marcy et al. (1978) 

Margrey et al. (1976) 

Mehrtens and Laturnus (1999) 

Meldrim et al. (1974; 1977; 1978) 

Meldrim and Flava (1977) 

Middaugh et al. (1977a and b; 1978) 

Morgan and Prince (1977; 1978) 

Mount and Quast (1993) 

Opresko (1982; 1983) 

Public Service Electric and Gas Company (1978) 

Roberts et al. (1975) 

Seegert and Brooks (1978) 

Stober and Hanson (1974) 

Thatcher (1979) 

Wilson (1977) 

Zhang and Minear (2002) 

Fish Behavior 

Hose et al. (1984) 

Fish Eggs 

Alderson (1969; 1972; 1974) 

Burton et al. (1979a and b) 

Hall et al. (1979b) 

Johnson et al. (1977)

Marcy (1973)

Marine Research Inc.(1976) 

Middaugh et al. (1977) 

Morgan and Prince (1977)

Rosales Casian (1991) 

Yosha and Cohen (1979) 

Fish Larvae 

Alderson (1969; 1972; 1974) 

91 
Burton et al. (1979a and b) 

Cakiroglu and Yurteri (1998) 

Enstrom and Kirkwood (1974) 

Goldman (1978) 

Hall et al. (1979b; 1981a and b; 1982) 

Holland et al. (1960) 

Hoss et al. (1977) 

Hughes (1972; 1975) 

Johnson et al. (1977) 

Marcy (1973) 

Marine Research Inc.(1976) 

Middaugh et al. (1977; 1978) 

Morgan and Prince (1977; 1978) 

Richkus and Lippson (1975) 

Rosales (1991) 

Rosales et al. (1990) 

Stober and Hanson (1974) 

Yosha and Cohen (1979) 

Woehrling and Le Fevre-Lehoerff (1997) 

Zhang and Minear (2002) 

General Environmental Effects

Hoepner and Lattemann (2003) 

Hydroid 

McLean (1972) 

Invertebrates 

Margrey et al. (1981) 

Other 

Khalanski and Bordet (1978)

Nicholson et al. (2003) 

Phytoplankton 

Ahamed et al. (1993) 

92 
Aprosi (1988) 

Azanza et al. (2001) 

Bender et al. (1977) 

Bongers et al. (1977) 

Brook and Baker (1972) 

Brooks (1974) 

Browdy et al. (1998) 

Burton and Margrey (1979) 

Carpenter et al. (1972; 1974b) 

Chang and Rossmann (1984) 

Davis and Coughlin (1978) 

Eppley et al. (1976) 

Erickson and Freeman (1978) 

Flemer (1974) 

Flemer and Sherk (1977) 

Fox and Moyer (1975) 

Gentile et al. (1976) 

Goldman and Quinby (1979) 

Goldman and Davidson (1979) 

Goldman et al. (1978) 

Hamilton et al. (1970) 

Harwood et al. (1989) 

Hall et al. (1981b; 1982) 

Hirayama and Hirano (1970)

Huang et al. (1997)

Junli et al. (1994) 

Laurer et al. (1974) 

Liden et al. (1980) 

Marcy et al. (1978) 

Marine Research Inc.(1976) 

Morgan and Stross (1969) 

Morgan et al. (1974) 

Mulford (1974) 

Nalewajko and Dunstall (1994) 

Opresko (1982; 1983) 

Peck and Warren (1978) 

Perissinotto and Wooldridge (1989) 

Rainville et al. (1975) 

Saravanane et al. (1998) 

Videau et al. (1979) 

Review

Abarnou and Miossec (1992) 

Capuzzo et al. (1977) 

93 
Chapman et al. (1995) 

Gavaskar and Chen (2002; 2005) 

Gentile et al. (1976) 

Haag (1981) 

Hall et al. (1981b; 1982) 

Hinchee (1998) 

Jolley (1984) 

Leberton (1976) 

Marcy (1973) 

Mattrice (1977) 

Mattice and Zittel (1976) 

Middaugh and Davis (1975) 

Opresko (1982; 1983) 

Pinkney (1992) 

Scott et al. (1989) 

Stanbro (1983) 

Wickramanayake and Gavaskar (2000) 

Winklehaus (1977) 

Viruses

Huang et al. (1997) 

Junli et al. (1994) 

Zooplankton 

Attrill et al. (1999) 

Bamber and Seaby (2004) 

Bender et al. (1977) 

Bongers et al. (1977) 

Bradley (1978) 

Burton and Margrey (1979) 

Carpenter et al. (1974a, b and c) 

Davies and Jensen (1975) 

Davis and Coughlin (1978) 

Dressel (1971) 

Erickson and Foulk (1980) 

Gaudy and Moatti (1978) 

Ginn and O'Connor (1978) 

Ginn et al. (1974) 

Goldman (1978) 

Goldman et al. (1978) 

Hall et al. (1981b; 1982) 

Heinle (1973; 1976) 

94 
Heinle and Beaven (1977) 

Heinle et al. (1974) 

Huang et al. (1997) 

Junli et al. (1994) 

Karaas (1992) 

Laurer et al. (1974) 

Lassus et al. (1981) 

Liden et al. (1979; 1980) 

Marcy et al. (1978) 

Markowski (1959) 

Marumo et al. (1992) 

Marine Research Inc. (1976) 

McLean (1973) 

Meldrim et al. (1974) 

Muchmore and Epel (1973) 

Opresko (1982; 1983) 

Perissinotto and Wooldridge (1989) 

Public Service Electric and Gas Company (1978) 

Richkus and Lippson (1975) 

Roberts et al. (1975) 

Roberts and Gleeson (1978) 

Thatcher (1978) 

Wilson (1977) 

Yang et al. (2002) 

95 
GENUS SPECIES INDEX 

Algae 

Gigartina pectinata (red algae) 

- Pacheco et al. (1997) 

Bacteria 

Escherichia coli 

- Azanza et al. (2001) 

- Bolster et al. (2005) 

Listeria monocytogenes 

- Iyer and Varma (1994) 

Propionigenium maris 

- Lovell (1999) 

Salmonella typhimurium (Ames strains - Mediterranean Sea) 

- Rav-Acha et al. (1991) 

Bivalves 

Brachidontes striatulus (brackish-water mussel) 

- Rajagopal et al. (1997a; 2003c; 2005) 

Brachidontes variabilius (mussel – tropical) 

- Rajagopal et al. (2003c; 2005) 

Crassostrea gigas (oyster) 

- Domart-Coulon et al. (2000) 

- Kim and Lee (1989) 

Crassostrea virginica (Americana oyster) 

- Block (1977) 

- Bongers et al. (1977) 

- Galstaff (1946) 

- Gibson et al. (1981) 

- Goldman (1978) 

- Liden et al. (1979; 1980) 

- Meldrim et al. (1974) 

- Rhoderick et al. (1977) 

- Roberts and Gleeson (1978) 

97 
- Scott and Middaugh (1978) 

- Stewart et al. (1979) 

Crassostrea madrasensis (oyster – tropical)

- Rajagopal et al. (2003b) 

Crassostrea virginica 

- Bongers et al. (1977) 

Donax serra (sandy beach bivalve) 

- Stenton-Dozey and Brown (1994) 

Dreissena polymorpha (zebra mussel) 

- Baier et al. (1994) 

- Bidwell et al. (1999) 

- Burton and Fisher (2001) 

- Ketelaars and Wagenvoort (1995) 

- Rajagopal et al. (2002a and b; 2003a and b) 

- Van Der Velde et al. (1997) 

Eliminus modestus (barnacle) 

- Waugh (1964) 

Haliotis cracherodii (black abalone) 

- Behrens and Larsson (1976) 

Haliotis refescens (red abalone) 

- Behrens and Larsson (1976) 

Mercenaria mercenaria (eastern hard clam) 

- Behrens and Larsson (1976) 

- Gibson et al. (1981) 

- Meldrim et al. (1974) 

Modiolus philippinarum (mussel – tropical) 

- Rajagopal et al. (2003c; 2005) 

Mullina lateralis (coot clam) 

- Roberts et al. (1979) 

Mya arenaria (soft shell clam) 

- Roosenburg et al. (1977) 

Mytilopsis leucohpaeata (dark false mussel) 

- Rajagopal et al. (1997b; 2002a and b; 2003a and b) 

- Van Der Velde et al. (1997) 

98 
Mytilus edulis (blue mussel) 

- Holmes (1969) 

- Kim and Lee (1989) 

- Morris et al. (1983) 

- Rajagopal et al. (2002; 2003 a and b) 

- Thompson et al. (1997) 

- White (1966; 1969) 

Mytilus sp.

- Beauchamp (1969) 

- James (1966) 

Ostrea edulis (European oyster) 

- Waugh (1964) 

Perna perna (mussel tropical) 

- Rajagopal et al. (2003c) 

- Xavier de Brito et al. (1999) 

Perna viridis (green mussel) 

- Masilamoni et al. (2002) 

- Rajagopal et al. (2003c) 

Protothaca stamineae (littleneck clam) 

- Gibson et al. (1981) 

Rangia cuneata (brackish water clam) 

- Bongers et al. (1977) 

- Liden et al. (1979; 1980) 

Ruditapes decussatus (clam) 

- Domart-Coulon et al. (2000) 

Tapes philippinarum 

- Kim and Lee (1989) 

Crustaceans 

Acartia clausi (copepod) 

- Gentile et al. (1976) 

Acartia omorii (copepod neritic marine) 

- Marumo et al. (1992) 

99 
Acartia tonsa (copepod) 

- Bamber and Seaby (2004) 

- Bender et al. (1977) 

- Bongers et al. (1977) 

- Bradley (1978) 

- Dressell (1971) 

- Gentile et al. (1976) 

- Heinle (1973; 1976) 

- Heinle and Beaven (1977) 

- Heinle et al. (1974) 

- Goldman (1978) 

- Goldman et al. (1978) 

- Laurer et al. (1974) 

- Liden et al. (1979; 1980) 

- McLean (1973) 

- Roberts and Gleeson (1978) 

- Roberts et al. (1975) 

Anonyx sp. (amphipod) 

- Thatcher (1978) 

Balanus (barnacles) 

- McLean (1973) 

Balanus improvisus 

- McLean (1973) 

Bosmina sp. (copepod) 

- Laurer et al. (1974) 

Brachionas plicatilis (rotifer) 

- Goldman (1978) 

- Goldman et al. (1978) 

Calanus pacificus (copepod) 

- Wilson (1977) 

Callinectes sapidus (blue crab)

- Block (1977) 

- Bongers et al. (1977) 

- Hall et al. (1979a) 

- Liden et al. (1979; 1980) 

- Meldrim et al. (1974) 

- Public Service Electric (1978) 

- Roberts et al. (1979) 

- Vreenegoor et al. (1977) 

100 
Cancer productus (crab) 

- Roesijadi et al. (1979) 

Carcinus maenas (crab) 

- Attrill et al. (1999) 

Cragnon crangon (decapod) 

- Attrill et al. (1999) 

- Bamber and Seaby (2004) 

Cragnon nigcrieaudo (shrimp) 

- Thatcher (1978) 

Cragnon septimspinosa (sand shrimp) 

- Meldrim et al. (1974) 

- Public Service Electric (1978) 

Dendraster excentricus (sand dollar) 

- Stober et al. (1978) 

Eurytemora affinis (copepod) 

- Bongers et al. (1977) 

- Bradley (1978) 

- Gentile et al. (1976) 

- Heinle (1976) 

- Laurer et al. (1974) 

Gammarus daiberi (amphipod) 

- Ginn and O'Connor (1978) 

- McLean (1973) 

- Public Service Electric (1978) 

Gammarus spp. (amphipod) 

- Ginn et al. (1974) 

- Laurer et al. (1974) 

- Margrey et al. (1981) 

- McLean (1973) 

Gammarus tigrinus (amphipod) 

- McLean (1973) 

Gammarus zaddachi (amphipod) 

- Attrill et al. (1999) 

- McLean (1973) 

101 
Halicyclops sp. (copepod) 

- Laurer et al. (1974) 

Hemigrapsus nudus (shore crab) 

- Thatcher (1978) 

Hemigrapus oregonensis (shore crab) 

- Thatcher (1978) 

Heptacarpus pictus (transparent shrimp) 

- Behrens and Larsson (1976) 

Homarus americanus (American lobster) 

- Capuzzo (1977) 

- Capuzzo et al. (1976b; 1977) 

- Enstrom and Kirkwood (1974) 

- Goldman (1978) 

Homarus gammarus (lobster) 

- Goldman (1978) 

Liocarcinus holsatus 

- Attrill et al. (1999) 

Melita nitida (amphipod) 

- McLean (1973) 

Monoculodes edwardsi (amphipod) 

- Laurer et al. (1974) 

Mysidopsis bahia (mysid shrimp) 

- Mount and Quast (1993) 

Neomysis americana (opossum shrimp) 

- Laurer et al. (1974) 

- Margrey et al. (1981) 

Neomysis sp. (shrimp) 

- Thatcher (1978) 

Pagurus longicarpus (crab) 

- Roberts (1978) 

Palaemon longirostris (prawn) 

- Attrill et al. (1999) 

102 
Palaemon serratus (prawn) 

- Attrill et al. (1999) 

- Lassus et al. (1981) 

Palamonetes pugio (grass shrimp) 

- Goldman (1978) 

- Hall et al. (1979a and c) 

- McLean (1973) 

- Meldrim et al. (1974) 

- Public Service Electric (1978) 

Pandalus danae (shrimp) 

- Block (1977) 

- Gibson et al. (1976) 

- Thatcher (1977; 1978) 

Pandalus goniurus (shrimp) 

- Thatcher (1978) 

Panopeus herbstii (crab) 

- Roberts (1978) 

Penaeus aztecus (brown shrimp) 

- Gibson et al. (1981) 

Penaeus kerathurus (shrimp) 

- Saragolia and Scarano (1979) 

Penaeus monodon (black tiger shrimp) 

- Graslund et al. (1999) 

Phragmatopoma californica (reef-building polychaete) 

- Muchmore and Epel (1973) 

Pontogeneia sp. (amphipod) 

- Thatcher (1978) 

Pseudodiaptomus coronatus (copepod) 

- Gentile et al. (1976) 

Scottolana canadensis (copepod) 

- Heinle (1973; 1976) 

Strongylocentrotus purpuratus (purple sea urchin) 

- Behrens and Larsson (1976) 

- Muchmore and Epel (1973) 

103 
Tegula pictus (brown turban snail) 

- Behrens and Larsson (1976) 

Urechis caupo (worm) 

- Muchmore and Epel (1973) 

Fish 

Alosa aestivalis (blueback herring) 

- Enstrom and Kirkwood (1974) 

- Morgan and Prince (1977) 

- Public Service Electric (1978) 

Alosa pseudoharengus (alewife) 

- Public Service Electric 

- Seegert and Brooks (1978) 

Alosa sapidissima (American shad) 

- Public Service Electric (1978) 

Ammodytes hexapterus (Pacific sand lance) 

- Thatcher (1978) 

Anchoa mitchilli (bay anchovy) 

- Public Service Electric (1978) 

Apeltes quadracus (fourspine stickleback) 

- Anderson et al. (1975) 

Brachydanio rerio (zebrafish) 

- Yosha and Cohen (1979) 

Brevoortia tyrannus (Atlantic menhaden) 

- Bongers et al. (1977) 

- Gibson et al. (1981) 

- Gullens et al. (1977) 

- Hall et al. (1982) 

- Hoss et al. (1975) 

- Liden and Burton (1977) 

- Liden et al. (1980) 

- Margrey et al. (1976) 

- Public Service Electric (1978) 

104 
Clupea harengus (Atlantic-Pacific herring) 

- Dempsey (1986) 

- Thatcher (1978) 

Cymatogaster aggregate (shinner perch) 

- Thatcher (1978; 1979) 

- Stober et al. (1978) 

Cynoscion regalis (weakfish) 

- Public Service Electric (1978) 

Cyprinodon variegates (sheepshead minnow) 

- Mount and Quast (1993) 

Engraulis mordax (northern anchovy) 

- Rosales (1991) 

Eucinostomus argentus (mojarra) 

- Hoss et al. (1977) 

Fundulus heteroclitus (mummichog) 

- Capuzzo et al. (1976a; 1977) 

- Gifts Bioassy work (1973) 

- Goldman (1978) 

- Goldman et al. (1978) 

- Meldrim et al. (1974) 

- Middaugh et al. (1978) 

- Public Service Electric (1978) 

Gadus morhua (Atlantic cod) 

- Mehrtens and Laturnus (1999) 

Gasterosteus aculeatus (threespine stickleback) 

- Thatcher (1978) 

Girella nigricans (opaleye-Pacific) 

- Hose et al. (1984) 

Gobiosoma bosci (naked gobi) 

- Bender et al. (1977) 

- Roberts et al. (1975) 

Hybognathus nuchalis (silvery minnow) 

- Public Service Electic (1977) 

105 
Lagodon rhomboids (pinfish) 

- Cripe (1979) 

Leiostomus xanthurus (spot) 

- Bellanca and Bailey (1977) 

- Bongers et al. (1977) 

- Liden and Burton (1977) 

- Liden et al. (1980) 

- Middaugh et al. (1977b) 

- Public Service Electric (1978) 

Leuresthes tenuis (grunion) 

- Rosales et al. (1990) 

Limanda limanda (dab-North Sea) 

- Mehrtens and Laturnus (1999) 

Lythrypnus dalli (blue-banded goby-Pacific) 

- Hose et al. (1984) 

Menidia beryllina (tidewater silverside) 

- Morgan and Prince (1977) 

- Public Service Electric (1978) 

Menidia menidia (Atlantic silverside) 

- Anderson et al. (1975) 

- Bender et al. (1977) 

- Bongers et al. (1977) 

- Enstrom and Kirkwood (1974) 

- Gift's Bioassay Work (1973) 

- Hoss et al. (1977) 

- Meldrim et al. (1974) 

- Meldrim and Flava (1977) 

- Morgan and Prince (1977) 

- Public Service Electric (1978) 

- Roberts et al. (1975) 

Micropogonias undulatus (Atlantic croaker) 

- Public Service Electric (1977) 

Morone americana (white perch) 

- Block et al. (1977; 1978) 

- Burton et al. (1979a and b) 

- Gullens et al. (1977) 

- Hall et al. (1979b; 1981a) 

106 
- Laurer’s Bioassay Work (1973) 

- Morgan and Prince (1977; 1978) 

Morone saxatilis (striped bass) 

- Burton et al. (1979a and b) 

- Hall et al. (1979b; 1981b) 

- Hughes (1972; 1975) 

- Middaugh et al. (1977a) 

- Morgan and Prince (1978) 

Mugil cephalus (striped mullet) 

- Hoss et al. (1975; 1977) 

Notropis hudsonius (spottail shiner) 

- Seegert and Brooks (1978) 

Oncorhynchus gorbuscha (pink salmon) 

- Holland et al. (1960) 

- Stober and Hanson (1974) 

- Thatcher (1978) 

Oncorhynchus kisutch (silver coho salmon) 

- Buckley (1976; 1977) 

- Buckley and Matsuda (1972; 1973) 

- Buckley et al. (1976) 

- Holland et al. (1960) 

- Seegert and Brooks (1978) 

- Thatcher (1978) 

Oncorhynchus tshawytscha (chinook salmon) 

 - Holland et al. (1960) 

- Stober and Hanson (1974) 

- Thatcher (1978) 

Osmerus morday (rainbow smelt) 

- Seegert and Brooks (1978) 

Paralichthys dentatus (summer flounder) 

- Goldman (1978) 

Paralichthys lethostigma (southern flounder) 

- Hoss et al. (1977) 

Paralichthys sp. (flounder) 

- Hoss et al. (1977) 

107 
Parophrys vetulus (English sole) 

- Thatcher (1978) 

Pleuronectes platessa (plaice) 

- Alderson (1969; 1972; 1974) 

- Mehrtens and Laturnus (1999) 

Pseudopleuronectes americanus (winter flounder) 

- Anderson et al. (1975) 

- Capuzzo et al. (1976a) 

- Gentile et al. (1976) 

- Goldman (1978) 

- Goldman et al. (1978) 

Sebastes mystinus (blue rockfish) 

- Wilson (1977) 

Solea solea (sole) 

- Alderson (1969; 1974) 

- Enstrom and Kirkwood (1974) 

Solea vulgaris (sole) 

- Woehrling and Le Fevre-Lehoerff (1997) 

Sprattus sprattus (sprat) 

- Woehrling and LeFevre-Lehoerff (1997) 

Stenotomus versicolor (scup) 

- Capuzzo et al. (1976a) 

- Goldman (1978) 

- Goldman et al. (1978) 

Syngnathus fuscus (northern pipefish) 

- Bender et al. (1977) 

- Roberts et al. (1975) 

Trinectes maculates (hogchoker) 

- Margrey et al. (1976) 

- Meldrim et al. (1974) 

- Public Service Electric (1978) 

Xiphister mucosus (rock prickleback) 

- Wilson (1977) 

108 
Hydroid 

Bimeria franciscana (colonial hydroid) 

- McLean (1972) 

Cordylophora caspra (brackish waster hydroid) 

- Rajagopal et al. (2002c) 

Phytoplankton 

Asterionella japonica 

- Gentile et al. (1976) 

Chactoceros decipiens 

- Gentile et al. (1976) 

Chactoceros didymum 

- Gentile et al. (1976) 

Chaetoceros gracilis

- Browdy et al. (1998) 

Chlamydomonas spp. 

- Hirayama and Hirano (1970) 

Coccochloris elebans 

- Rainville et al. (1975) 

Detonula confervacca 

- Gentile et al. (1976) 

Dunaliella primolecta

- Videau et al. (1979) 

Dunaliella tertiolecta

- Gentile et al. (1976) 

- Sikka et al. (1979) 

Glenodinium halli 

- Erickson and Freeman (1978) 

Isochrysis galbana 

- Erickson and Freeman (1978) 

109 
Microcrystis aeruginosa

- Sikka et al. (1979) 

Monochrysis luther 

- Gentile et al. (1976) 

- Lamont-Doherty Bioassay Work (1973) 

Nannochloris occulatus 

- Bender et al. (1977) 

Pavlova luther

- Videau et al. (1979) 

Phaeodactylum tricornutum

- Goldman and Davidson (1977) 

- Goldman et al. (1978) 

- Videau et al. (1979) 

Porphyridium sp. 

- Sikka et al. (1979) 

Pseudoisoochrysis paradoxa 

- Bender et al. (1977) 

Pyraminonas virginicu 

- Bender et al. (1977) 

Pyrodinium bahamense

- Azanza et al. (2001) 

Rhodomonas baltica 

- Gentile et al. (1976) 

Scenedesmus obliguus

- Sikka et al. (1979) 

Selenastrum capricornutum

- Harwood et al. (1989) 

- Sikka et al. (1979) 

Skeltonema costatum 

- Erickson and Freeman (1978) 

- Gentile et al. (1976) 

- Hirayama and Hirano (1970) 

- Lamont-Doherty Bioassay Work (1973) 

- Sikka et al. (1979) 

110 
Tetraselmia syecica 

- Bender et al. (1977) 

Thalassiosira nordensholdii 

- Gentile et al. (1976) 

 

Thalassiosira pseudonana

- Erickson and Freeman (1978) 

- Gentile et al. (1976) 

- Sikka et al. (1979) 

Thalassiosira rotula 

- Gentile et al. (1976) 

Thalassiosira sp. 

-Saravanane et al. (1998) 

111 
The Department of the Interior Mission

As the Nation's principal conservation agency, the Department of the Interior has responsibility 

for most of our nationally owned public lands and natural resources. This includes fostering 

sound use of our land and water resources; protecting our fish, wildlife, and biological diversity; 

preserving the environmental and cultural values of our national parks and historical places; 

and providing for the enjoyment of life through outdoor recreation. The Department assesses 

our energy and mineral resources and works to ensure that their development is in the best 

interests of all our people by encouraging stewardship and citizen participation in their care. 

The Department also has a major responsibility for American Indian reservation communities 

and for people who live in island territories under U.S. administration. 

The Minerals Management Service Mission 

As a bureau of the Department of the Interior, the Minerals Management Service's (MMS) 

primary responsibilities are to manage the mineral resources located on the Nation's Outer 

Continental Shelf (OCS), collect revenue from the Federal OCS and onshore Federal and Indian 

lands, and distribute those revenues. 

Moreover, in working to meet its responsibilities, the Offshore Minerals Management Program 

administers the OCS competitive leasing program and oversees the safe and environmentally 

sound exploration and production of our Nation's offshore natural gas, oil and other mineral 

resources. The MMS Minerals Revenue Management meets its responsibilities by ensuring the 

efficient, timely and accurate collection and disbursement of revenue from mineral leasing and 

production due to Indian tribes and allottees, States and the U.S. Treasury. 

The MMS strives to fulfill its responsibilities through the general guiding principles of: (1) being 

responsive to the public's concerns and interests by maintaining a dialogue with all potentially 

affected parties and (2) carrying out its programs with an emphasis on working to enhance the 

quality of life for all Americans by lending MMS assistance and expertise to economic 

development and environmental protection.