# Patent

## Title
Process for regioselective mono-tosylation of diols

## Abstract

The present invention concerns the use of dibutyl tin oxide for regioselective catalytic diol mono-tosylation at a concentration lower than 2 mol %. The present invention also concerns the use of a generic acetal compound of Formula (Ic) in a catalytic process for regioselective diol mono-tosylation, wherein Y is selected from the group of C1-6alkyl, phenyl and benzyl. The concentration of the generic acetal compound of Formula (Ic) is less than about 2 mol %, preferably ranges between about 2 mol % and about 0.0005 mol %, preferably ranges between about 0.1 mol % and about 0.005 mol %.

## Background
CROSS REFERENCE TO RELATED APPLICATIONS
This application is the national stage of PCT Application No. PCT/EP2007/062109, filed Nov. 9, 2007, which claims priority from European Patent Application No. 06124292.1, filed Nov. 17, 2006, the entire disclosures of which are hereby incorporated in their entirety.
INTRODUCTION
It is well known in the art that the concentration of impurities, in particular metal impurities in the production of API's (active pharmaceutical intermediates) should be as low as possible to avoid labour-intensive and expensive purification methods, such as e.g. recrystallisation. This restriction poses severe limitations to the use of reagents in metal-catalysed synthesis processes of API's and it is generally desired to use only to a limited extent reagents and intermediates based on metals, in particular heavy metals. One such reaction that is commonly used, is the regioselective diol mono-tosylation reaction. Such a reaction may be performed without the use of a metal-containing catalyst, however, in a number of instances, low conversion and/or selectivity (defined as the ratio of mono- to di-tosylated product), is commonly observed.
The use of dibutyl tin oxide 1 (Bu2SnO) is well known for the regioselective derivatization of vicinal diols 2. Since Shanzer's original paper (Shanzer, A. Tet. Letters 1980, 21, 221), the stoechiometric process has been widely applied in sugar chemistry (David, S.; Hanessian, S. Tetrahedron 1985, 41, 643 and Walkup, R. E.; Vernon, N. M. Wingard, R. E., Jr. Patent Application EP 448413 A1, 1991). The reaction can be generalized to functionalize other diols as well (Boons, G.-J.; Castle, G. H.; Clase, A.; Grice, P.; Ley, S. V.; Pinel, C. Synlett 1993, 913). Unfortunately, since the reaction is stoechiometric, it requires a high amount of dibutyl tin oxide, which further needs to be separated from the reaction mixture.
The catalytic process, described in 1999 by Martinelli and co-workers (Martinelli, M. J.; Nayyar, N. K.; Moher, E. D.; Dhokte, U. P.; Pawlak, J. M.; Vaidyanathan, R. Org. Letters 1999, 1, 447) allows the selective mono-tosylation of a diol with only 2 mol % of Bu2SnO in the presence of a classical base like triethylamine (Et3N). In a more recent paper (Martinelli, M. J.; Vaidyanathan, R.; Pawlak, J. M.; Nayyar, N. K.; Dhokte, U. P.; Doecke, C. W.; Zollars, L. M. H.; Moher, E. D.; Khau, V. V.; Kom{hacek over (s)}mrlj, B. J. Am. Chem. Soc. 2002, 124, 3578), the mechanistic aspects of the reaction were detailed (Scheme 1). The intermediate formation of the corresponding tin acetal 3 was postulated, which is also in accordance with the reactivity of tin acetals isolated in the stoechiometric process.




Tin acetal intermediates like 3 are considered to be more reactive than 1 and to give more reproducible results. In a recent paper, Fasoli et al. (Fasoli, E. Caligiuri, A.; Servi, S.; Tessaro, D. J. Mol. Cat. A 2006, 244, 41) described mono-benzoylation of a diol with catalytic Sn acetal derived either from the reacting diol itself, or from alcohols like methanol or isopropanol.
The inventors have now found that in the above catalytic process of regioselective mono-tosylation of vicinal diols, the amount of dibutyl tin oxide 1 can surprisingly be reduced from the disclosed 2 mol % to 0.1 mol %, without any loss of conversion and selectivity.
The inventors have also found that a generic Sn-acetal, advantageously independent of the reacting diol itself, and advantageously in a solid form, can be used in the above catalytic process of regioselective mono-tosylation of vicinal diols, preferably at concentrations well below 2 mol %, in particular below 0.1 mol %, and even down to 0.001 mol % to yield a very high conversion and selectivity. The process is applicable on different commercial diol substrates and has the major advantage that only trace amounts of Sn (lower ppm-range) remain in the API.


DESCRIPTION OF THE INVENTION
The present invention concerns the use of dibutyl tin oxide for regioselective catalytic diol mono-tosylation at a concentration lower than 2 mol %, preferably in the range between 2 mol % and about 0.05 mol %, inclusive, in particular about 0.1 mol %.
The present invention also concern a process for the regioselective catalytic diol mono-tosylation, comprising a step wherein a compound comprising a diol moiety of Formula (Ia) is tosylated into a compound comprising a tosylated diol moiety of Formula (Ib) using less than 2 mol % of Y2SnO,




wherein

Y is selected from the group of C1-6alkyl, phenyl and benzyl;

X is selected from the group of Cl, Br, and OTs; and

n is an integer equal to 1 or 2.

In one embodiment, the concentration of Y2SnO ranges between 2 mol % and about 0.05 mol %, inclusive. In another embodiment, the concentration of Y2SnO is about 0.1 mol %.
The present invention also concerns the use of a generic acetal compound of Formula (Ic) in a catalytic process for regioselective diol mono-tosylation,




wherein Y is selected from the group of C1-6alkyl, phenyl and benzyl.

The generic acetal compound of Formula (Ic) can be made according to preparation methods well-known in the art, for example, according to methods disclosed in EP 448413 B1 (Noramco Inc), which are herein included by reference. The acetal compound of Formula (Ic) may also be formed in situ when compounds of Formula (Ia) are tosylated. However, it is preferred that the acetal compound of Formula (Ic) is different from the acetal compound formed in the regioselective diol mono-tosylation reaction.
The present invention also concerns the use of a generic acetal compound of Formula (Ic) in a catalytic process for regioselective diol mono-tosylation, wherein Y is selected from the group of C1-6alkyl, phenyl and benzyl, wherein the concentration of the compound of Formula (Ic) is less than about 2 mol %, preferably ranges between about 2 mol % and about 0.0005 mol %, preferably ranges between about 0.1 mol % and about 0.005 mol %.
The present invention also concern a process for the catalytic regioselective diol mono-tosylation, wherein a compound comprising a diol moiety of Formula (Ia) is tosylated into a compound comprising a tosylated diol moiety of Formula (Ib) using a generic acetal compound of Formula (Ic), wherein




Y is selected from the group of C1-6alkyl, phenyl and benzyl;

X is selected from the group of Cl, Br, and OTs; and

n is an integer equal to 1 or 2.

According to one embodiment, the concentration of the generic acetal compound of Formula (Ic) is less than about 2 mol %, preferably ranges between about 2 mol % and about 0.0005 mol %, preferably ranges between about 0.1 mol % and about 0.005 mol %.
According to another embodiment, Y is butyl. According to another embodiment, the generic acetal compound of Formula (Ic) is 2,2-dibutyl-[1,3,2]dioxastannolane, which is a commercially available raw material.
Within the framework of this application, tosyl is an abbreviation for p-toluenesulfonate. It is the conjugate base of the strong acid, p-toluenesulfonic acid. The tosyl group, like other sulfonates, is a highly reactive leaving group. The process of introducing a tosyl-group into a molecule, is called mono-tosylation.
Within the framework of this application, a diol moiety is a moiety comprising at least two hydroxy groups, separated by at least 2 and at most 3 carbon atoms.
Within the framework of this application, “regioselective” means that the reaction takes place only at the primary alcohol.
Results and Discussion
A. Preparation of acetic acid 8-fluoro-11-[2-hydroxy-3-(toluene-4-sulfonyloxy)-propyl]-10,11-dihydro-5H-dibenzo[a,d]cyclohepten-10-yl ester (Compound 6a)




Compound 2a was tosylated using a variety of conditions. In short (lab procedure (0.1 mol scale): To compound 2a (34.4 g, 0.1 mol) in toluene (1.2 L/mol) compound 1 (various amounts) is added at 25° C. The mixture is stirred during 1 hour. Diisopropyl ethylamine, triethylamine or pyridine (various amounts) is added and the reaction mixture is stirred during 5 min. Tosyl chloride (various amounts) is added and the reaction mixture is stirred at that temperature during 16 hours. Hydrochloric acid 1N (150 ml) is added and the mixture is stirred vigorously. pH of the aqueous phase is 1 to 2; the organic phase is filtered over sodium sulfate and used further in the next step.
Example 17 was further conducted on a pilot plant scale (35 mol scale): To compound 2a in toluene (34 L) compound 1 (9 g, 0.25 g/mol) is added and the mixture is stirred at 25° C. during 1 hour. N,N-diisopropyl ethyl amine (5.0 kg, 38.4 mol) is added, followed by tosyl chloride (7.0 kg, 36.8 mol). After stirring the reaction mixture at 25° C. during 16 hours, HCl 1N (1.35 eq.) in water is added. The pH of the aqueous layer is 1 to 2. The organic layer is dried over Na2SO4 (3.5 kg) and used as such in the next step. The estimated yield was 80%.
The results are shown in Table 1. Compounds according to Formula 2a and 6a are known from Mao, Hua; Koukni, Mohamed; Kozlecki, Tomasz; Compernolle, Frans; Hoornaert, Georges J. Diastereoselective synthesis of trans-fused tetrahydropyran derivatives of 5H-dibenzo[a,d]cycloheptene. Tetrahedron Letters (2002), 43(48), 8697-8700. From the Table 1 can be seen that the amount of Bu2SnO in the catalyzed mono-tosylation reaction could be lowered down to as low as 0.1 mol %. Unlike Martinelli (Martinelli, M. J.; Nayyar, N. K.; Moher, E. D.; Dhokte, U. P.; Pawlak, J. M.; Vaidyanathan, R. Org. Letters 1999, 1, 447.), some unidentified impurities were formed when Et3N was used, while diisopropyl ethyl amine (i-Pr2NEt, Hünig's base) gave cleaner reaction. We acknowledge however that this latter point might strongly depend on the substrate used.














TABLE 1











Con-




Bu2SnO
TsCl
pyridine
Et3N
i-Pr2NEt
version
Selectivity*


Ex.
(mol %)
(eq.)
(eq.)
(eq.)
(eq.)
(%)
(mono:di)






















1
0
1.5
10
—
—
92
 86:14


2
10
1.5
—
1.5
—
>99
 90:10


3
5
1.05
—
1.05
—
>99
96:4


4
2
1.05
—
1.05
—
>99
96:4


5
2
1.1
—
1.1
—
>99
94:6


6
1
1.05
—
1.05
—
>99
94:6


7
1
1
—
1.05
—
87
92:8













8
0
1.05
—
—
1.2
Only 60% conversion








after 4 days














9
1
1.1
—
—
1.15
93
91:9


10
0.5
1
—
—
1.05
97
98:2


11
0.5
1.1
—
—
1.2
95
>99:1 


12
0.5
1.05
—
—
1.2
>99
>99:1 


13
0.1
1.05
—
—
1.2
97
96:4













14
0.05
1.05
—
—
1.2
Only 50% conversion








after 16 hours at r.t.





*HPLC, area %. Samples were analyzed after 1 hour reaction, unless stated otherwise. No favourable evolution is observed after 3, 6 or 24 hours.






B. Preparation of 2,2-dibutyl[1,3,2]dioxastannolane
The tin acetal 2,2-dibutyl[1,3,2]dioxastannolane was prepared from Bu2SnO and ethylene glycol according to the following procedure (scheme 3)




The following experiment was only performed at lab scale (0.1 mol). Compound 1 (24.9 g, 0.1 mol) is dissolved in toluene (100 ml, 1 L/mol). Ethylene glycol (28 ml, 5 eq.) is added at 25° C. Water is removed azeotropically at 110-114° C. and the reaction mixture is stirred at that temperature during 5 hours. After gradual cooling (110° C.→20° C. over 12 hours), the precipitate 3a is filtered, washed and dried at 40° C. under vacuum. Yield: 27.4 g (93%). Compound 3b is used as such for further experiments. For analytical purposes, a 10 g sample is recrystallised in toluene (40 ml, 4 ml/g) with gradual cooling (110° C.→20° C. over 10 hours). Anal. Calcd. for C10H22O2Sn: C, 41.00; H, 7.57. Found: C, 40.68; H, 7.70.
NMR 1H-CDCl3: 0.9 (t, 6H), 1.3 (m, 4H), 1.4 (m, 4H), 1.63 (m, 4H), 3.62 (s, 4H)
C. Regioselective diol mono-tosylation Using the Generic Acetal Compound 3a
The generic acetal compound 3a was used to perform the mono-tosylation reaction according Scheme 2 for a series of compounds. In short, a mixture of the corresponding diol, stannylene acetal 3a, iPr2NEt and TsCl in various amounts was stirred at room temperature for 16 hours. Hydrochloric acid 1N (1.5 eq.) was added and the mixture was stirred vigorously. The organic phase was filtered over sodium sulfate and used further as such in the next step.
The results are shown in Table 2. From the Table 2 can be seen that the amount of compound 3a in the catalyzed mono-tosylation reaction could be lowered down to as low as 0.001 mol %. This presents a major improvement over the prior art preparation methods and allows for the production of API's with very low Sn concentrations.










TABLE 2









Bu2SnO (mol %)





















0.1
0.1
0.05
0.01
0.005
0.001
0.0005











Conversion (%)


Nr.
Substrate
Selectivity (mono:di)


















1




97 96:4
97 96:4
97 >99:1
—
84 >99:1
—
—


 


2




96 95:5
97 97:3
97 97:3
92 98:2
94 95:5
85 95:5 
81 89:11


 


3




95 99:1
97 >99:1
98 >99:1
92 99:1
87 99:1
70 96:4 
—


 


4




95 99:1
95 99:1
95 98:2
96 95:5
92 93:7
63 70:30
—


 


5*




93
88
88
84
83
 0
—


 


6*




85
86
86
68
—
—
—





Substrates 1 to 4: % conversion and selectivity is based on HPLC area %.


Substrates 5 and 6: % conversion and selectivity is based on GC area %.


*The GC chromatogram didn't show any ditosylation or starting material, analysis with HPLC showed some ditosylation.

## Claims

1. Process for the catalytic mono-tosylation of a diol, comprising a step wherein a compound comprising a diol moiety of Formula (Ia) is tosylated into a compound comprising a tosylated diol moiety of Formula (Ib) using a compound of Formula (Ic)




wherein Y is selected from the group of C1-6alkyl, phenyl, and benzyl, in a concentration between 2 mol % and 0.0005 mol %,




wherein
X is selected from the group of Cl, Br, and OTs; and
n is an integer equal to 0 or 1.

2. The process as claimed in claim 1 wherein the concentration of the compound of Formula (Ic) is between 0.1 mol % and 0.005 mol %.

3. The process as claimed in claim 2 wherein the compound of Formula (Ic) is 2,2-dibutyl-[1,3,2]dioxastannolane.