### SAT Report PMN Number: P-12-0069 SAT Date: 12/9/2011 Print Date: 2/27/2015

Related cases : Yes Health related cases: Ecotox related cases: Analogs:

.

Concern levels : Type of Concern: Health Eco Comments Level of Concern: 2 2

Persistence Bioaccum Toxicity Comments 1 1 1 Anion 1 1 1 Cation

Exposure Based Review : Health: Ecotox: N o
Routes of exposure : Health: Dermal Inhalation Ecotox: All releases to water Fate: ; Anion rapid; Cation rapid
Keywords : Keywords: LUNG; IRR/CORR-E,S,L,MM; N EURO; AQUATOX
Summary of Assessment :

Fate : Fate Summary: P-12-0069-70 FATE: Estimations for the typical covalent ion pair MW Liquid with MP < 25 C (E) log Kow = 8.77 (E) S = Disp. / <0.001 mg /L at 25 C (ICB/E) VP < 1.0E-6 torr at 25 C (E) BP > 400 C (E) H < 1.00E-8 (E) log Koc = 5.04 (E) log Fish BCF = 1.85 (E)log Fish BAF = 5.61 (E) POTW removal (%) = Anion 90 via sorption and biodeg; Cation 0-90; OECD 301B(Mod Sturm CO2 ev): 94.2%/28d. Time for complete ultimate aerobic biodeg = Anion wk; Cation wk-mo Sorption to soils/sediments = Anion low; Cation low PBT Potential: Anion P1B1; Cation P1B1 *CEB FATE: Migration to ground water = Anion rapid; Cation rapid

Health : Health Summary: Absorption is poor from the skin, moderate from the GI tract, and good from the lung (analog). Concern for surfactant effects on the lung; irritation to eye, skin (chronic), mucous membranes and lung based on surfactant properties of the compounds and test data on an analogue. Also concern for neurotoxicity for the small

Test Data: [Analog: ]: (-) Salmonella with and without activation; rat oral LD50 >500, <1000 mg/kg; rat dermal LD0 = 2000 mg/kg; Corrosive in an in vitro rat skin disc assay

Ecotox :
Test Organism Test Type Test End Point Predicted Measured Comments

fish 96-h LC50 50 62 Analog data daphnid 48-h LC50 6.1 > 100 Analog data green algal 96-h EC50 4.7 19 Analog data fish

_ chronic value 2.6 daphnid

_ chronic value 0.52

algal

_ chronic value 1.6 2.7 Analog data

Sewage Sludge 3-h EC50

_ Sewage Sludge

_ Chronic Value _

Ecotox Values Comments: Predictions are based on SARs for with molecular weight adjustment; SAR chemical class = ; ; liquid with mp unknown (P); S = dispersible in water at 25 C (P); p H7; effective concentrations based on 100% active ingredients and mean measured concentrations; hardness <150.0 mg/L as CaCO3; and TOC <2.0 mg/L;

P-12-0069: . with (CASRN: N ONE)

Three acute ecotoxicity tests were conducted on an analog of PMN (P-12-0069) in 2005 by with fish, daphnia, and algae. The compound tested wasand is considered an acceptable analog (by Gordon Cash) for P-12-0069. P-12-0069 is a liquid with an estimated boiling point is > 400 °C. The estimated vapor pressure is < 0.000001 mm Hg at 25 °C. The solubility of the P-12-0069 is dispersible. These studies complied with OCSPP and OECD guidelines for: 1) O2, p H, temperature, water hardness values and other water quality parameters; 2) species, age, number of organisms per replicate, and biomass loading rates; and, 3) replicate and overall variability. The test was p erformed in a and there was a quality assurance statement provided.

In a 96-hour fish test, (10/ two replicate/test concentration) were exposed
to as water accommodation fraction (WAF) of 6.3, 13, 25, 50, and 100 mg/L as nominal concentrations with static-renewal exposures. The test protocol was amended by aerating the test chambers throughout the 96-hour exposure period. Also, the test chambers were 25-L stainless steel aquaria containing 11.5 L of test solution rather than the specified in the protocol. test solutions were mixed directly with dilution water on a weight volume basis . Each was stirred for approximately 24 hours in a 13.2 L pyrex aspirator bottle using a lined magnetic stir bar to create a vortex depth of approximately 30% of the test solution height. After mixing, the were allowed to settle for approximately one hour. The test solutions were then drawn from spigots p laced near the bottom of the mixing vessels and added to the appropriate test chamber. The fish were transferred to newly prepared test solutions at 48 hours, and at test termination and analyzed using was measured using a -5000 total . There was no mortality in the control or 6.3

– 25 mg/L treatment groups. Mortality in the 50 and 100 mg/L treatment groups was 20 and 100%, respectively. The 96-hour LL50 for is 62 mg/L. The 96-hour NOEL for is 25 mg/L. The P test is valid.

In a 48-hour immobilization toxicity test , Daphnia magna (10 daphnia/two replicates/test concentration) were exposed to as WAF of 6.3, 13, 25, 50, and 100 mg/L with static-renewal exposures. test solutions were mixed directly with dilution water on a weight volume basis. Each 12-L was stirred for approximately 24 hours in a 13.2 L pyrex aspirator bottle using a lined magnetic stir bar to create a vortex depth of approximately 30% of the test solution height. After mixing, the s were allowed to settle for approximately one hour. The test solutions were then drawn from spigots placed near the bottom of the mixing vessels and added to the appropriate test chamber. was renewed at 24 hours, and at test termination and analyzed using was measured using a total . Observations of immobility were made at 3, 24, and 48 hours after test initiation. The 48-hour EL50 for is > 100 mg/L. The NOEL of is 6.3 mg/L. The D. magna test is valid.

In a 72-hour algal growth inhibition test, (1 x 104 cells/mL) were exposed to at concentrations of 1.8, 4.1, 10, 20, 45, and 100 mg/L as nominal concentrations under static conditions. The pH ranged from 7.7 to 7.9. Three replicates were used for each of the test concentrations and 6 replicates for the control. Over the course of the algal study, the temperature remained at 23.0°C. The 72-hour ErL50 (growth rate) and EbL50 (biomass) of for P. subcapitata are 72 and 19 mg/L, respectively. The 72-hour NOEL for biomass was <1.8 mg/L. The 72-hour NOEL based ongrowth rate for is 1.8 mg/L. The 72-hour LOEL based on biomass for is 1.8 mg/L. The 72-hour LOEL for growth rate for is 4.1 mg/L. The calculated geometric mean of the LOEL and NOEL (the algal chronic value or algal ChV), based on growth rate for is 2.7 mg/L. The toxicity test is valid.

All three submitted aquatic ecotoxicity studies are valid. Since is a suitable analog for P-12-0069, the aquatic toxicity endpoint results from can be used to assess the aquatic toxicity endpoints for P-12-0069. For comparative purposes, the ECOSAR (v. 1.1) p redicted fish 96-hour LC50, Daphnia 48-hour EC50 and algal 72-hour EC50 are 50, 6.1, and 4.7 mg/L for P-12-0069, respectively. In addition, the ECOSAR predicted chronic value for fish, daphnid and algae is 2.6, 0.52, and 1.6 mg/L. The acute concentration of concern is derived by dividing the 72-hour algal EC50 (19 mg/L or 19,000 p pb), the most sensitive aquatic species of the three submitted, by an uncertainty factor of 4 (for algae) to yield 4,750 p pb. The chronic concentration of concern is derived by dividing the predicted Daphnid ChV (0.52 mg/L), more sensitive than the experimental algal ChV of , by an uncertainty factor of 10, yielding 0.052 mg/L or 52 p pb.

Acute CoC = 4,750 p pb Chronic CoC = 52 p pb

Ecotox Study Reviewer: Amuel Kennedy QA/QC: Jeff Gallagher 14 December 2011

Factors Values Comments Assessment Factor 10 Concentration of Concern ( p pb) 52

SARs with molecular weight adjustment SAR Class Ecotox Category

Ecotox Factors Comments:

SAT Chair: L Keifer 564-8916