Federal Register / Vol. 76, No. 37 / Thursday, February 24, 2011 / Notices 10379 

production of a digestion product with 

a homogeneous C-terminus. 

Applications 

• Removal short C-terminal affinity 

tags from recombinant proteins without 

leaving any nonnative residues behind 

when used in combination with 

MeCPA. 

• Identification and removal of 

disordered residues from the C-termini 

of native (untagged) proteins, thereby 

increasing their propensity to 

crystallize. 

Inventors: David Waugh et al. (NCI) 

Related Publications: None. 

Patent Status: HHS Reference No. E– 

027–2011/0—Research Tool. Patent 

protection is not being pursued for this 

technology. 

Licensing Status: Available for 

licensing. 

Licensing Contact: Whitney Hastings; 

301–451–7337; hastingw@mail.nih.gov. 

Collaborative Research Opportunity: 

The National Cancer Institute, Protein 

Engineering Section, is seeking 

statements of capability or interest from 

parties interested in collaborative 

research to further develop, evaluate, or 

commercialize recombinant BoCPB and/ 

or similar enzymes. Please contact John 

Hewes, PhD at 301–435–3121 or 

hewesj@mail.nih.gov for more 

information. 

A DsbC Expression Vector for the 

Production of Proteins With Disulfide 

Bonds in the Cytosol of E. coli 

Description of Technology: Many 

proteins of biomedical importance 

contain disulfide bonds and such 

proteins are notoriously difficult to 

produce in Escherichia coli. Current 

methods to address this problem either 

export the protein to the periplasmic 

space, which is a more favorable redox 

environment for disulfide bond 

formation, or utilize genetically 

modified strains of E. coli to alter the 

redox potential of the cytosol (such as 

‘‘Origami’’ or ‘‘Shuffle’’ cells). 

Unfortunately, these methods generally 

result in very low yields of the desired 

product, thus emphasizing the need for 

a novel method. 

The NIH inventors have designed a 

DsbC expression vector that can be used 

to improve the yield of correctly 

oxidized recombinant proteins in the 

cytosol of E. coli. By overproducing 

DsbC on a separate plasmid and 

coexpressing it with carboxypeptidases 

in the cytosol of E. coli, the inventors 

were able to increase the amount of 

properly oxidized, active 

carboxypeptidases that could be 

recovered from the cytosol by at least 4- 

fold. Further, they believe that coexpression of DsbC from a multicopy 

plasmid vector will also improve the 

yield of other disulfide bond-containing 

proteins in E. coli. 

Applications: Improving the yield of 

correctly oxidized recombinant proteins 

in the cytosol of E. coli. 

Advantages: Substantial increase in 

the amount of active carboxypeptidases 

recovered from the cytosol and 

improved yield of disulfide bondcontaining proteins in E. coli. 

Inventors: David Waugh et al. (NCI) 

Related Publications 

1. Prinz WA, Aslund F, Holmgren A, 

Beckwith J. The role of the thioredoxin 

and glutaredoxin pathways in reducing 

protein disulfide bonds in the 

Escherichia coli cytoplasm. J Biol Chem. 

1997 Jun 20;272(25):15661–15667. 

[PubMed: 9188456] 

2. Levy R, Weiss R, Chen G, Iverson 

BL, Georgiou G. Production of correctly 

folded Fab antibody fragment in the 

cytoplasm of Escherichia coli trxB gor 

mutants via the coexpression of 

molecular chaperones. Protein Expr 

Purif. 2001 Nov;23(2):338–347. 

[PubMed: 11676610] 

Patent Status: HHS Reference No. E– 

028–2011/0—Research Tool. Patent 

protection is not being pursued for this 

technology. 

Licensing Status: Available for 

licensing. 

Licensing Contact: Whitney Hastings; 

301–451–7337; hastingw@mail.nih.gov. 

Dated: February 16, 2011. 

Richard U. Rodriguez, 

Director, Division of Technology Development 

and Transfer, Office of Technology Transfer, 

National Institutes of Health. 

[FR Doc. 2011–4168 Filed 2–23–11; 8:45 am] 

BILLING CODE 4140–01–P 

DEPARTMENT OF HEALTH AND 

HUMAN SERVICES 

National Institutes of Health 

Laboratory Animal Welfare: Proposed 

Adoption and Implementation of the 

Eighth Edition of the Guide for the 

Care and Use of Laboratory Animals 

AGENCY: National Institutes of Health, 

HHS. 

ACTION: Notice. 

SUMMARY: The National Institutes of 

Health (NIH) requests public comments 

on (1) NIH’s adoption of the eighth 

edition of the Guide for the Care and 

Use of Laboratory Animals (Guide) as a 

basis for evaluation of institutional 

programs receiving or proposing to 

receive Public Health Service (PHS) 

support for activities involving animals; 

and (2) if NIH decides to adopt the 

eighth edition of the Guide, NIH’s 

proposed implementation plan, which 

would require that institutions complete 

at least one semiannual program and 

facility evaluation using the eighth 

edition of the Guide as the basis for 

evaluation by March 31, 2012. NIH will 

consider comments on (1) the adoption 

of the Guide and (2) the implementation 

plan. 

DATES: Written comments on the 

adoption and implementation of the 

eighth edition of the Guide must be 

received by NIH within 30 days of the 

date of publication of this notice in 

order to be considered. 

ADDRESSES: Public comments may be 

entered at 

http://grants.nih.gov/grants/olaw/ 

2011guidecomments/add.htm. 

Comments will be made publicly 

available. Personally identifiable 

information (except organizational 

affiliations) will be removed prior to 

making comments publicly available. 

FOR FURTHER INFORMATION CONTACT: 

Office of Laboratory Animal Welfare, 

Office of Extramural Research, National 

Institutes of Health, RKL1, Suite 360, 

6705 Rockledge Drive, Bethesda, MD 

20892–7982; telephone 301–496–7163. 

SUPPLEMENTARY INFORMATION: 

I. Background 

The Guide, first published in 1963, is 

a widely accepted primary reference on 

animal care and use. Recommendations 

in the Guide are based on published 

data, scientific principles, expert 

opinion, and experience with methods 

and practices that are determined to be 

consistent with high quality, humane 

animal care and use. The eighth edition 

of the Guide was published in January 

2011 following a study by the Institute 

for Laboratory Animal Research of the 

National Academy of Sciences (NAS). 

The NAS study process began in 2008 

and followed the requirements of 

Section 15 of the Federal Advisory 

Committee Act. The NAS study process 

is described at the NAS Web site: http:// 

www.nationalacademies.org/ 

studyprocess/index.html. 

Since 1985, the PHS Policy on 

Humane Care and Use of Laboratory 

Animals, authorized by Public Law 99– 

158, 42 U.S.C. 289d, and incorporated 

by reference at 42 CFR 52.8 and 42 CFR 

52a.8, has required that institutions 

receiving PHS support for animal 

activities base their animal care and use 

programs on the current edition of the 

Guide and comply, as applicable, with 

the Animal Welfare Act and other 

Federal statutes and regulations relating 


emcdonald on DSK2BSOYB1PROD with NOTICES
10380 Federal Register / Vol. 76, No. 37 / Thursday, February 24, 2011 / Notices 

to animal activities. The PHS Policy is 

applicable to all PHS-conducted or 

-supported activities (including 

research, research training, 

experimentation, biological testing, or 

related purposes) involving live 

vertebrate animals. 

The eighth edition of the Guide 

contains substantive changes and 

additions from the previous edition. To 

gain insight from institutions on the 

impact of changes to the Guide on their 

animal care and use programs, NIH 

seeks comments on whether it should 

adopt the eighth edition of the Guide. 

NIH simultaneously proposes an 

implementation plan for the eighth 

edition of the Guide and seeks 

comments on the proposed plan. 

The implementation plan proposed by 

NIH would require institutions to 

complete at least one semiannual 

program and facility evaluation, using 

the eighth edition of the Guide as the 

basis for evaluation, by March 31, 2012. 

For such an evaluation to be considered 

complete by NIH, it would need to 

include reasonable and specific plans 

and schedules for corrections of 

deficiencies where appropriate. 

II. Electronic Access 

The eighth edition of the Guide is 

available on the NIH Office of 

Laboratory Animal Welfare Web site at 

http://olaw.nih.gov. 

Dated: February 16, 2011. 

Francis S. Collins, 

Director, National Institutes of Health. 

[FR Doc. 2011–4172 Filed 2–23–11; 8:45 am] 

BILLING CODE 4140–01–P 

DEPARTMENT OF HEALTH AND 

HUMAN SERVICES 

National Institutes of Health 

National Institute of Mental Health; 

Notice of Meeting 

Pursuant to section 10(a) of the 

Federal Advisory Committee Act, as 

amended (5 U.S.C. App.), notice is 

hereby given of a meeting of the 

Interagency Autism Coordinating 

Committee (IACC) Subcommittee on 

Safety. 

The IACC Subcommittee on Safety 

will be having a conference call on 

Wednesday, March 16, 2011. The 

subcommittee plans to discuss safety 

issues related to autism spectrum 

disorder (ASD). This meeting will be 

accessible to the public through a 

conference call. 

Name of Committee: Interagency Autism 

Coordinating Committee (IACC). 

Type of meeting: Subcommittee on Safety. 

Date: March 16, 2011. 

Time: 9 a.m. to 10:30 a.m. Eastern Time. 

Agenda: The subcommittee plans to 

discuss safety issues related to autism 

spectrum disorder. 

Place: No in-person meeting; conference 

call only. 

Conference Call Access: Dial: 888–390– 

3417. Access code: 4684708. 

Contact Person: Ms. Lina Perez, Office of 

Autism Research Coordination, National 

Institute of Mental Health, NIH, 6001 

Executive Boulevard, NSC, Room 8185a, 

Rockville, MD 20852, Phone: 301–443–6040, 

E-mail: IACCPublicInquiries@mail.nih.gov. 

Please Note: The conference call will be 

accessible to the public through a conference 

call-in number and access code. Members of 

the public who participate using the 

conference call phone number will be able to 

listen to the meeting but will not be heard. 

If you experience any technical problems 

with the conference call, please e-mail 

IACCTechSupport@acclaroresearch.com or 

call the IACC Technical Support Help Line 

at 443–680–0098. 

Individuals who participate by using this 

electronic service and who need special 

assistance, such as captioning of the 

conference call or other reasonable 

accommodations, should submit a request to 

the Contact Person listed on this notice at 

least 7 days prior to the meeting. 

Schedule subject to change. 

Information about the IACC and a 

registration link for this meeting are available 

on the Web site: http://www.iacc.hhs.gov. 

Dated: February 17, 2011. 

Jennifer S. Spaeth, 

Director, Office of Federal Advisory 

Committee Policy. 

[FR Doc. 2011–4164 Filed 2–23–11; 8:45 am] 

BILLING CODE 4140–01–P 

DEPARTMENT OF HEALTH AND 

HUMAN SERVICES 

National Institutes of Health 

National Institute of Mental Health; 

Notice of Closed Meetings 

Pursuant to section 10(d) of the 

Federal Advisory Committee Act, as 

amended (5 U.S.C. App.), notice is 

hereby given of the following meetings. 

The meetings will be closed to the 

public in accordance with the 

provisions set forth in sections 

552b(c)(4) and 552b(c)(6), Title 5 U.S.C., 

as amended. The grant applications and 

the discussions could disclose 

confidential trade secrets or commercial 

property such as patentable material, 

and personal information concerning 

individuals associated with the grant 

applications, the disclosure of which 

would constitute a clearly unwarranted 

invasion of personal privacy. 

Name of Committee: National Institute of 

Mental Health Special Emphasis Panel; 

National Cooperative Drug Discovery 

Development Groups. 

Date: March 10, 2011. 

Time: 10 a.m. to 12 p.m. 

Agenda: To review and evaluate grant 

applications. 

Place: National Institutes of Health, 

Neuroscience Center, 6001 Executive 

Boulevard, Rockville, MD 20852, (Telephone 

Conference Call). 

Contact Person: Vinod Charles, PhD, 

Scientific Review Officer, Division of 

Extramural Activities, National Institute of 

Mental Health, NIH, Neuroscience Center, 

6001 Executive Blvd., Room 6151, MSC 9606, 

Bethesda, MD 20892–9606, 301–443–1606, 

charlesvi@mail.nih.gov. 

Name of Committee: National Institute of 

Mental Health Special Emphasis Panel; Viral 

and Host Genetics in NeuroAIDS. 

Date: March 16, 2011. 

Time: 12 p.m. to 5 p.m. 

Agenda: To review and evaluate grant 

applications. 

Place: National Institutes of Health, 

Neuroscience Center, 6001 Executive 

Boulevard, Rockville, MD 20852, (Telephone 

Conference Call). 

Contact Person: David W Miller, PhD, 

Scientific Review Officer, Division of 

Extramural Activities, National Institute of 

Mental Health, NIH, Neuroscience Center, 

6001 Executive BLVD, Room 6140, MSC 

9608, Bethesda, MD 20892–9608, 301–443– 

9734, millerda@mail.nih.gov. 

(Catalogue of Federal Domestic Assistance 

Program Nos. 93.242, Mental Health Research 

Grants; 93.281, Scientist Development 

Award, Scientist Development Award for 

Clinicians, and Research Scientist Award; 

93.282, Mental Health National Research 

Service Awards for Research Training, 

National Institutes of Health, HHS) 

Dated: February 17, 2011. 

Jennifer S. Spaeth 

Director, Office of Federal Advisory 

Committee Policy. 

[FR Doc. 2011–4166 Filed 2–23–11; 8:45 am] 

BILLING CODE 4140–01–P 

DEPARTMENT OF HEALTH AND 

HUMAN SERVICES 

National Institutes of Health 

National Institute of General Medical 

Sciences; Notice of Closed Meeting 

Pursuant to section 10(d) of the 

Federal Advisory Committee Act, as 

amended (5 U.S.C. App.), notice is 

hereby given of the following meeting. 

The meeting will be closed to the 

public in accordance with the 

provisions set forth in sections 

552b(c)(4) and 552b(c)(6), Title 5 U.S.C., 

as amended. The grant applications and 

the discussions could disclose 

confidential trade secrets or commercial 

property such as patentable material, 

and personal information concerning 

individuals associated with the grant 


emcdonald on DSK2BSOYB1PROD with NOTICES