# Patent

## Title
Bacillus licheniformis chromosome

## Abstract

The present invention relates to an isolated polynucleotide of the complete chromosome of Bacillus licheniformis. The present invention also relates to isolated genes of the chromosome of Bacillus licheniformis which encode biologically active substances and to nucleic acid constructs, vectors, and host cells comprising the genes as well as methods for producing biologically active substances encoded by the genes and to methods of using the isolated genes of the complete chromosome of Bacillus licheniformis.

## Background
CROSS-REFERENCE TO RELATED APPLICATIONS
This application claims the benefit of U.S. Provisional Application No. 60/535,988, filed Jan. 9, 2004, U.S. Provisional Application No. 60/561,059, filed Apr. 8, 2004, and U.S. Provisional Application No. 60/572,403, filed May 18, 2004, which applications are incorporated herein by reference.


BACKGROUND OF THE INVENTION
1. Field of the Invention
The present invention relates to an isolated polynucleotide molecule comprising the complete chromosome of Bacillus licheniformis. The present invention also relates to features (genes) of the complete chromosomal DNA molecule of Bacillus licheniformis which encode biologically active substances and to nucleic acid constructs, vectors, and host cells comprising the features as well as methods for producing biologically active substances encoded by the features and to methods of using the isolated features derived from the complete chromosomal DNA molecule of Bacillus licheniformis. 
2. Description of the Related Art
Microbes, which make up most of the earth's biomass, have evolved for some 3.8 billion years. They are found in virtually every environment, surviving and thriving in extremes of heat, cold, radiation, pressure, salt, acidity, and darkness. Often in these environments, no other forms of life are found and the only nutrients come from inorganic matter. The diversity and range of their environmental adaptations indicate that microbes long ago “solved” many problems for which scientists are still actively seeking solutions. The value in determining the complete genome sequence of microbes is that it provides a detailed blueprint for the organism revealing all of the biochemical pathways, substrates, intermediates, and end products as well as regulatory networks, and evolutionary relationships to other microbes. A complete manifest of proteins, both structural and catalytic, is encoded as a list of features in the DNA molecule comprising the genome, as well as their likely cellular location.
Knowledge about the enormous range of microbial capacities has broad and far-reaching implications for environmental, energy, health, and industrial applications, such as cleanup of toxic-waste, production of novel therapeutic and preventive agents (drugs and vaccines), energy generation and development of renewable energy sources, production of chemical catalysts, reagents, and enzymes to improve efficiency of industrial processes, management of environmental carbon, nitrogen and nutrient cycling, detection of disease-causing organisms and monitoring of the safety of food and water supplies, use of genetically altered bacteria as living sensors (biosensors) to detect harmful chemicals in soil, air, or water, and understanding of specialized systems used by microbial cells to live in natural environments.
Bacillus licheniformis is a gram positive spore-forming bacterium that is widely distributed as a saprophytic organism in the environment. Unlike most other bacilli that are predominantly aerobic, Bacillus licheniformis is a facultative anaerobe which may allow it to grow in additional ecological niches. This species produces a diverse assortment of extracellular enzymes that are believed to contribute to the process of nutrient cycling in nature (Claus, D. and Berkeley, R. C. W., 1986, In Bergey's Manual of Systematic Bacteriology, Vol. 2., eds. Sneath, P. H. A. et al., Williams and Wilkins Co., Baltimore, Md., pp. 1105-1139). Certain Bacillus licheniformis isolates are capable of denitrification, however, the relevance of this characteristic to environmental denitrification may be small since the species generally persists in soil as endospores (Alexander, M., 1977, Introduction to Soil Microbiology. John Wiley and Sons, Inc., New York).
There are numerous industrial and agricultural uses for Bacillus licheniformis and its extracellular products. The species has been used for decades in the manufacture of industrial enzymes including several proteases, α-amylase, penicillinase, pentosanase, cycloglucosyltransferase, β-mannanase, and several pectinolytic enzymes, owing largely to its ability to secrete sizeable amounts of degradative enzymes. Bacillus licheniformis is also used to produce peptide antibiotics such as bacitracin and proticin, in addition to a number of specialty chemicals such as citric acid, inosine, inosinic acid, and poly-γ-glutamic acid. The proteases from Bacillus licheniformis are used in the detergent industry as well as for dehairing and batting of leather (Eveleigh, D. E., 1981, Scientific American 245, 155-178). Amylases from Bacillus licheniformis are deployed for the hydrolysis of starch, desizing of textiles, and sizing of paper (Erickson, R. J., 1976, In Microbiology, ed. Schlesinger, D. (Am. Soc. Microbiol., Washington, D.C.), pp. 406-419.). Certain strains of Bacillus licheniformis have shown efficacy to destroy fungal pathogens affecting maize, grasses, and vegetable crops (U.S. Pat. Nos. 5,589,381; 5,665,354). As an endospore-forming bacterium, the ability of the organism to survive under unfavorable environmental conditions may enhance its potential as a natural control agent.
Bacillus licheniformis can be differentiated from other bacilli on the basis of metabolic and physiological tests (Logan, N. A. and Berkeley, R. C. W., 1981, In The Aerobic Endospore-Forming Bacteria: Classification and Identification, eds. Berkeley, R. C. W. and Goodfellow, M., Academic Press, Inc., London, pp. 106-140; O'Donnell, A. G., Norris, J. R., Berkeley, R. C. W., Claus, D., Kanero, T., Logan, N. A., and Nozaki, R., 1980, Internat. J. Systematic Bacteriol. 30: 448-459). However, biochemical and phenotypic characteristics may be ambiguous among closely related species. Lapidus et al. (Lapidus, A., Galleron, N., Andersen, J. T., Jørgensen, P. L. Ehrlich, S. D., and Sorokin, A., 2002, FEMS Microbiol. Lett. 209: 23-30) recently constructed a physical map of the Bacillus licheniformis chromosome using a PCR approach, and established a number of regions of co-linearity where gene content and organization were ostensibly conserved with the Bacillus subtilis chromosome.
It would be advantageous to the art to have available the complete primary structure of the chromosomal DNA molecule of the Bacillus licheniformis type strain ATCC 14580. With the complete chromosome data in hand, it should be possible to do comparative genomics and proteomics studies that can lead to improved industrial strains as well as to a better understanding of genome evolution among closely-related bacilli in the subtilis-licheniformis group.
It is an object of the present invention to provide an isolated polynucleotide with the sequence of the complete chromosome of Bacillus licheniformis. 
SUMMARY OF THE INVENTION
The present invention relates to an isolated polynucleotide of the complete chromosomal DNA molecule of Bacillus licheniformis ATCC 14580 having the nucleotide sequence of SEQ ID NO: 1.
The present invention also relates to isolated features (genes) of the complete chromosomal DNA molecule of Bacillus licheniformis ATCC 14580 encoding biologically active substances, selected from the group consisting of:
(a) a gene comprising a nucleotide sequence having at least 60% identity with any of the polynucleotides of SEQ ID NOs: 2-4198; and
(b) a gene comprising a nucleotide sequence which hybridizes under at least medium stringency conditions with any of the polynucleotides of SEQ ID NOs: 2-4198, or a complementary strand thereof.
The present invention also relates to biologically active substances encoded by the isolated genes, and nucleic acid constructs, vectors, and host cells comprising the genes.
The present invention also relates to methods for producing such substances having biological activity comprising (a) cultivating a recombinant host cell comprising a nucleic acid construct comprising a gene encoding the biologically active substance under conditions suitable for production of the biologically active substance; and (b) recovering the biologically active substance.
The present invention also relates to methods for monitoring differential expression of a plurality of genes in a first bacterial cell relative to expression of the same genes in one or more second bacterial cells, comprising:
(a) adding a mixture of detection reporter-labeled nucleic acids isolated from the bacterial cells to a substrate containing an array of Bacillus licheniformis genes selected from the group consisting of nucleotides SEQ ID NOs: 2-4198, complementary strands of SEQ ID NOs: 2-4198, or fragments of SEQ ID NOs: 2-4198, under conditions where the detection reporter-labeled nucleic acids hybridize to complementary sequences of the Bacillus licheniformis genes on the array, wherein the nucleic acids from the first bacterial cell and the one or more second bacterial cells are labeled with a first detection reporter and one or more different second detection reporters, respectively; and
(b) examining the array under conditions wherein the relative expression of the genes in the bacterial cells is determined by the observed detection signal of each spot on the array in which (i) the Bacillus licheniformis genes on the array that hybridize to the nucleic acids obtained from either the first or the one or more second bacterial cells produce a distinct first detection signal or one or more second detection signals, respectively, and (ii) the Bacillus licheniformis genes on the array that hybridize to the nucleic acids obtained from both the first and one or more second bacterial produce a distinct combined detection signal.
The present invention also relates to methods for isolating a gene encoding an enzyme, comprising:
(a) adding a mixture of labeled first nucleic acid probes, isolated from a microbial strain cultured on medium without an inducing substrate, and labeled second nucleic acid probes, isolated from the microbial strain cultured on medium with the inducing substrate, to an array of Bacillus licheniformis genes selected from the group consisting of nucleotides SEQ ID NOs: 2-4198, complementary strands of SEQ ID NOs: 2-4198, or fragments of SEQ ID NOs: 2-4198, under conditions where the labeled nucleic acid probes hybridize to complementary sequences of the Bacillus licheniformis genes on the array, wherein the first nucleic acid probes are labeled with a first reporter and the second nucleic acid probes are labeled with a second reporter;
(b) examining the array under conditions wherein the relative expression of the genes of the microbial strain is determined by the observed hybridization reporter signal of each spot on the array in which (i) the Bacillus licheniformis genes on the array that hybridize to the first nucleic acid probes produce a distinct first hybridization reporter signal or the second nucleic acid probes produce a distinct second hybridization reporter signal, and (ii) the Bacillus licheniformis genes on the array that hybridize to both the first and second nucleic acid probes produce a distinct combined hybridization reporter signal; and
(c) isolating a gene from the microbial strain that encodes an enzyme that degrades or converts the substrate.
The present invention also relates to genes isolated by such methods and nucleic acid constructs, vectors, and host cells containing the genes.
DEFINITIONS
Biologically active substance: The term “biologically active substance” is defined herein as any substance which is encoded by a single gene or a series of genes (contiguous or non-contiguous) composing a biosynthetic or metabolic pathway or operon or may be the direct or indirect result of the product of a single gene or products of a series of genes of the Bacillus licheniformis chromosome. Such substances include, but are not limited to, biopolymers, metabolites, and cellular structures and components (e.g., ribosome, flagella, etc.). For purposes of the present invention, biological activity is determined according to procedures known in the art such as those described by Carpenter and Sabatini, 2004, Nature 5: 11-22; Sordie et al., 2003, Proceedings of the National Academy of Sciences USA 100: 11964-11969; Braun and LaBaer, 2003, TRENDS in Biotechnology 21: 383-388; and Kaberdin and McDowall, 2003, Genome Research 13: 1961-1965.
In the methods of the present invention, the biopolymer may be any biopolymer. The term “biopolymer” is defined herein as a chain (or polymer) of identical, similar, or dissimilar subunits (monomers). The biopolymer may be, but is not limited to, a nucleic acid, polyamine, polyol, polypeptide (or polyamide), or polysaccharide.
In a preferred aspect, the biopolymer is a polypeptide. The polypeptide may be any polypeptide having a biological activity of interest. The term “polypeptide” is not meant herein to refer to a specific length of the encoded product and, therefore, encompasses peptides, oligopeptides, and proteins. The term “polypeptide” also encompasses naturally occurring allelic variations.
In a preferred aspect, the polypeptide is an antibody, antigen, antimicrobial peptide, enzyme, growth factor, hormone, immunodilator, neurotransmitter, receptor, reporter protein, structural protein, transcription factor, and transporter.
In a more preferred aspect, the polypeptide is an oxidoreductase, transferase, hydrolase, lyase, isomerase, or ligase. In a most preferred aspect, the polypeptide is an alpha-glucosidase, aminopeptidase, amylase, carbohydrase, carboxypeptidase, catalase, cellulase, chitinase, cutinase, cyclodextrin glycosyltransferase, deoxyribonuclease, esterase, alpha-galactosidase, beta-galactosidase, glucoamylase, glucocerebrosidase, alpha-glucosidase, beta-glucosidase, invertase, laccase, lipase, mannosidase, mutanase, oxidase, pectinolytic enzyme, peroxidase, phospholipase, phytase, polyphenoloxidase, proteolytic enzyme, ribonuclease, transglutaminase, urokinase, or xylanase.
In another preferred aspect, the polypeptide is a collagen or gelatin.
In another preferred aspect, the biopolymer is a polysaccharide. The polysaccharide may be any polysaccharide, including, but not limited to, a mucopolysaccharide (e.g., heparin and hyaluronic acid) and a nitrogen-containing polysaccharide (e.g., chitin). In a more preferred aspect, the polysaccharide is hyaluronic acid (hyaluronan).
In the methods of the present invention, the metabolite may be any metabolite. The metabolite may be encoded by one or more genes, such as a biosynthetic or metabolic pathway. The term “metabolite” encompasses both primary and secondary metabolites. Primary metabolites are products of primary or general metabolism of a cell, which are concerned with energy metabolism, growth, and structure. Secondary metabolites are products of secondary metabolism (see, for example, R. B. Herbert, The Biosynthesis of Secondary Metabolites, Chapman and Hall, New York, 1981).
The primary metabolite may be, but is not limited to, an amino acid, fatty acid, nucleoside, nucleotide, sugar, triglyceride, or vitamin.
The secondary metabolite may be, but is not limited to, an alkaloid, coumarin, flavonoid, polyketide, quinine, steroid, peptide, or terpene. In a preferred aspect, the secondary metabolite is an antibiotic, antifeedant, attractant, bacteriocide, fungicide, hormone, insecticide, or rodenticide.
Isolated biologically active substance: The term “isolated biologically active substance” is defined herein as a substance which is at least about 20% pure, preferably at least about 40% pure, more preferably about 60% pure, even more preferably about 80% pure, most preferably about 90% pure, and even most preferably about 95% pure, as determined by SDS-PAGE, HPLC, capillary electrophoresis, or any other method used in the art.
Substantially pure biologically active substance or pure biologically active substance: The term “substantially pure biologically active substance” is defined herein as a biologically active substance preparation which contains at most 10%, preferably at most 8%, more preferably at most 6%, more preferably at most 5% by weight, more preferably at most 4%, at most 3%, even more preferably at most 2%, most preferably at most 1%, and even most preferably at most 0.5% by weight of other material with which it is natively associated. It is, therefore, preferred that the substantially pure biologically active substance is at least 92% pure, preferably at least 94% pure, more preferably at least 95% pure, more preferably at least 96% pure, more preferably at least 96% pure, more preferably at least 97% pure, even more preferably at least 98% pure, most preferably at least 99%, and even most preferably at least 99.5% pure by weight of the total material present in the preparation. The term “pure biologically active substance” is defined as a biologically active substance preparation which contains no other material with which it is natively associated.
The biologically active substances of the present invention are preferably in a substantially pure form. In particular, it is preferred that the biologically active substances are in “essentially pure form”, i.e., that the biologically active substance preparation is essentially free of other material with which it is natively associated. This can be accomplished, for example, by preparing the biologically active substance by means of well-known recombinant methods or by classical purification methods.
Identity: The relatedness between two amino acid sequences or between two nucleotide sequences is described by the parameter “identity”.
For purposes of the present invention, the degree of identity between two amino acid sequences is determined by the Smith-Waterman Protein method for the Genematcher2, as implemented by Paracel Inc. (Pasadena, Calif.), or the BLASTP method as described by Altschul et al., 1990, Journal of Molecular Biology 215: 403-410.
For purposes of the present invention, the degree of identity between two nucleotide sequences is determined by the Smith Waterman nucleotide method for the Genematcher2 or BLASTN for the BlastMachine as implemented by Paracel Inc.
Polypeptide Fragment: The term “polypeptide fragment” is defined herein as a polypeptide, which retains biological activity, having one or more amino acids deleted from the amino and/or carboxyl terminus of a polypeptide encoded by any of the genes of the present invention, i.e., polypeptides of SEQ ID NOs: 4199-8395. Preferably, a fragment contains at least 80%, preferably at least 85%, more preferably at least 90%, even more preferably at least 95%, and most preferably at least 97% of the amino acid residues of the mature encoded polypeptide product.
Subsequence: The term “subsequence” is defined herein as a polynucleotide comprising a nucleotide sequence of any of SEQ ID NOs: 2-4198 except that one or more nucleotides have been deleted from the 5′ and/or 3′ end. Preferably, a subsequence contains at least 80%, preferably at least 85%, more preferably at least 90%, even more preferably at least 95%, and most preferably at least 97% of the nucleotides of any of the genes of the present invention.
Allelic variant: The term “allelic variant” denotes herein any of two or more alternative forms of a gene occupying the same chromosomal locus. Allelic variation arises naturally through mutation, and may result in polymorphism within populations. Gene mutations can be silent (no change in the encoded polypeptide) or may encode polypeptides having altered amino acid sequences. An allelic variant of a polypeptide is a polypeptide encoded by an allelic variant of a gene.
Substantially pure polynucleotide or pure polynucleotide: The term “substantially pure polynucleotide” as used herein refers to a polynucleotide preparation free of other extraneous or unwanted nucleotides and is in a form suitable for use within genetically engineered production systems. Thus, a substantially pure polynucleotide contains at most 10%, preferably at most 8%, more preferably at most 6%, more preferably at most 5%, more preferably at most 4%, more preferably at most 3%, even more preferably at most 2%, most preferably at most 1%, and even most preferably at most 0.5% by weight of other polynucleotide material with which it is natively associated. A substantially pure polynucleotide may, however, include naturally occurring 5′ and 3′ untranslated regions, such as promoters and terminators. It is preferred that the substantially pure polynucleotide is at least 92% pure, preferably at least 94% pure, more preferably at least 95% pure, moire preferably at least 96% pure, more preferably at least 96% pure, more preferably at least 97% pure, more preferably at least 98% pure, most preferably at least 99%, and even most preferably at least 99.5% pure by weight. The polynucleotides of the present invention are preferably in a substantially pure form. In particular, it is preferred that the polynucleotides disclosed herein are in “essentially pure form”, i.e., that the polynucleotide preparation is essentially free of other polynucleotide material with which it is natively associated. The term “pure polynucleotide” is defined as a polynucleotide preparation which contains no other material with which it is natively associated.
Nucleic acid construct: The term “nucleic acid construct” as used herein refers to a nucleic acid molecule, either single- or double-stranded, which is isolated from a naturally occurring gene or which has been modified to contain segments of nucleic acids in a manner that would not otherwise exist in nature. The term nucleic acid construct is synonymous with the term “expression cassette” when the nucleic acid construct contains the control sequences required for expression of a coding sequence of the present invention.
Control sequence: The term “control sequences” is defined herein to include all components, which are necessary or advantageous for the expression of a biologically active substance of the present invention. Each control sequence may be native or foreign to the polynucleotide encoding the substance. Such control sequences include, but are not limited to, a leader, propeptide sequence, promoter, signal peptide sequence, and transcription terminator. At a minimum, the control sequences include a promoter, and transcriptional and translational stop signals. The control sequences may be provided with linkers for the purpose of introducing specific restriction sites facilitating ligation of the control sequences with the coding region of the polynucleotide encoding a biologically active substance.
Operably linked: The term “operably linked” as used herein refers to a configuration in which a control sequence is placed at an appropriate position relative to the coding sequence of the DNA sequence, such that the control sequence directs the expression of a biologically active substance.
Coding sequence: When used herein the term “coding sequence” is intended to cover a nucleotide sequence, which directly specifies the amino acid sequence of its protein product. The boundaries of the coding sequence are generally determined by an open reading frame, which usually begins with the ATG start codon or alternative start codons such as GTG and TTG.
Expression: The term “expression” includes any step involved in the production of a biologically active substance including, but not limited to, transcription, post-transcriptional modification, translation, post-translational modification, and secretion.
Expression vector: The term “expression vector” herein covers a DNA molecule, linear or circular, that comprises a segment encoding a biologically active substance of the invention, and which is operably linked to additional segments that provide for its transcription.
Host cell: The term “host cell”, as used herein, includes any cell type which is susceptible to transformation, transfection, conjugation, electroporation, etc. with a nucleic acid construct, plasmid, or vector.


DETAILED DESCRIPTION OF THE INVENTION
Bacillus licheniformis Chromosome and Features (Genes) Thereof
The present invention relates to an isolated polynucleotide of the complete chromosomal DNA molecule of Bacillus licheniformis ATCC 14580 having the nucleotide sequence of SEQ ID NO: 1. Bacillus licheniformis ATCC 14580, consists of a circular molecule of 4,222,336 base pairs with a mean G+C content of 46.2%. The chromosome contains 4208 predicted protein-coding genes (SEQ ID NOs: 2-4198) with an average size of 873 bp, 7 rRNA operons, and 72 tRNA genes. The deduced amino acid sequences of the 4208 predicted protein-coding genes are shown in SEQ ID NOs: 4199-8395. SEQ ID NO: 4210 corresponds to SEQ ID NO: 2. SEQ ID NO: 4211 corresponds to SEQ ID NO: 3; SEQ ID NO: 4212 corresponds to SEQ ID NO: 4, etc. The predicted functions of the 4208 gene products are shown in Table 1.
The Bacillus licheniformis chromosome possesses regions that are markedly co-linear with the chromosomes of Bacillus subtilis and Bacillus halodurans, and approximately 80% of the predicted genes have Bacillus subtilis orthologues.
The present invention also relates to isolated features (genes) of the complete chromosomal DNA molecule of Bacillus licheniformis ATCC 14580 encoding biologically active substances, selected from the group consisting of:
(a) a gene comprising a nucleotide sequence having at least 60% identity with any of the nucleotide sequences of SEQ ID NOs: 2-4198; and
(b) a gene comprising a nucleotide sequence which hybridizes under at least medium stringency conditions with any of the genes of SEQ ID NOs: 2-4198, or a complementary strand thereof.
In a first aspect, the present invention relates to isolated genes, which have a degree of identity to the nucleotide sequences of any of SEQ ID NOs: 2-4198 of at least about 60%, preferably at least about 65%, more preferably at least about 70%, more preferably at least about 75%, more preferably at least about 80%, more preferably at least about 85%, even more preferably at least about 90%, most preferably at least about 95%, and even most preferably at least about 97%, which encode biologically active substances having a particular biological activity (hereinafter “homologous biologically active substances”).
In a second aspect, the present invention relates to isolated genes comprising nucleotide sequences which hybridize under very low stringency conditions, preferably low stringency conditions, more preferably medium stringency conditions, more preferably medium-high stringency conditions, even more preferably high stringency conditions, and most preferably very high stringency conditions with any of (i) the genes of SEQ ID NOs: 2-4198, or subsequences thereof, or (ii) complementary strands thereof (J. Sambrook, E. F. Fritsch, and T. Maniatus, 1989, Molecular Cloning, A Laboratory Manual, 2d edition, Cold Spring Harbor, N.Y.). Subsequences of SEQ ID NOs: 2-4198 may be at least 100 nucleotides or preferably at least 200 nucleotides. Moreover, the subsequences may encode fragments of a gene product which have biological activity. The biologically active substances may also be biologically active allelic variants of the biologically active substances.
The nucleotide sequences of SEQ ID NOs: 2-4198 or subsequences thereof, as well as the amino acid sequences of SEQ ID NOs: 4199-8395 or fragments thereof, may be used to design nucleic acid probes to identify and clone DNA encoding biologically active substances from strains of different genera or species according to methods well known in the art. In particular, such probes can be used for hybridization with the genomic DNA of the genus or species of interest, following standard Southern blotting procedures, in order to identify and isolate the corresponding gene therein. Such probes can be considerably shorter than the entire sequence, but should be at least 14, preferably at least 25, more preferably at least 35 nucleotides in length, such as at least 70 nucleotides in length. It is preferred, however, that the nucleic acid probes are at least 100 nucleotides in length. For example, the nucleic acid probes may be at least 200 nucleotides, at least 300 nucleotides, at least 400 nucleotides, or at least 500 nucleotides in length. Even longer probes may be used, e.g., nucleic acid probes which are at least 600 nucleotides, at least 700 nucleotides, at least 800 nucleotides, or at least 900 nucleotides in length. Both DNA and RNA probes can be used. The probes are typically labeled for detecting the corresponding gene (for example, with 32P, 3H, 35S, biotin, or avidin). Such probes are encompassed by the present invention.
A genomic DNA library prepared from such other organisms may, therefore, be screened for DNA which hybridizes with the probes described above and which encodes a biologically active substance. Genomic DNA from such other organisms may be separated by agarose or polyacrylamide gel electrophoresis, or other separation techniques. DNA from the libraries or the separated DNA may be transferred to and immobilized on nitrocellulose or other suitable carrier material. In order to identify a clone or DNA which is homologous with any of SEQ ID NOs: 2-4198 or subsequences thereof, the carrier material is used in a Southern blot.
For purposes of the present invention, hybridization indicates that a polynucleotide hybridizes to a labeled gene having the nucleotide sequence shown in any of SEQ ID NOs: 2-4198, complementary strands thereof, or subsequences thereof, under very low to very high stringency conditions. Molecules to which the nucleic acid probe hybridizes under these conditions can be detected using X-ray film.
In a preferred aspect, the nucleic acid probe is any of the genes of SEQ ID NOs: 2-4198, or subsequences thereof. In another preferred aspect, the nucleic acid probe is the mature coding region of any of the genes of SEQ ID NOs: 2-4198. In another preferred aspect, the nucleic acid probe is the gene of any of SEQ ID NOs: 2-4198 contained in Bacillus licheniformis ATCC 14580. In another preferred aspect, the nucleic acid probe is the mature coding region of any of the genes of SEQ ID NOs: 2-4198 contained in Bacillus licheniformis ATCC 14580.
For long probes of at least 100 nucleotides in length, very low to very high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 μg/ml sheared and denatured salmon sperm DNA, and either 25% formamide for very low and low stringencies, 35% formamide for medium and medium-high stringencies, or 50% formamide for high and very high stringencies, following standard Southern blotting procedures.
For long probes of at least 100 nucleotides in length, the carrier material is finally washed three times each for 15 minutes using 2×SSC, 0.2% SDS preferably at least at 45° C. (very low stringency), more preferably at least at 50° C. (low stringency), more preferably at least at 55° C. (medium stringency), more preferably at least at 60° C. (medium-high stringency), even more preferably at least at 65° C. (high stringency), and most preferably at least at 70° C. (very high stringency).
For short probes which are about 14 nucleotides to about 70 nucleotides in length, stringency conditions are defined as prehybridization, hybridization, and washing post-hybridization at about 5° C. to about 10° C. below the calculated Tm using the calculation according to Bolton and McCarthy (1962, Proceedings of the National Academy of Sciences USA 48:1390) in 0.9 M NaCl, 0.09 M Tris-HCl pH 7.6, 6 mM EDTA, 0.5% NP-40, 1× Denhardt's solution, 1 mM sodium pyrophosphate, 1 mM sodium monobasic phosphate, 0.1 mM ATP, and 0.2 mg of yeast RNA per ml following standard Southern blotting procedures.
For short probes which are about 14 nucleotides to about 70 nucleotides in length, the carrier material is washed once in 6×SCC plus 0.1% SDS for 15 minutes and twice each for 15 minutes using 6×SSC at 5° C. to 10° C. below the calculated Tm.
Under salt-containing hybridization conditions, the effective Tm is what controls the degree of identity required between the probe and the filter bound DNA for successful hybridization. The effective Tm may be determined using the formula below to determine the degree of identity required for two DNAs to hybridize under various stringency conditions.

Effective Tm=81.5+16.6(log M[Na+])+0.41(% G+C)−0.72(% formamide)

The % G+C content of any of the genes of SEQ ID NOs: 2-4198 can easily be determined. For medium stringency, for example, the concentration of formamide is 35% and the Na+ concentration for 5×SSPE is 0.75 M. Applying this formula to these values, the Effective Tm in ° C. can be calculated. Another relevant relationship is that a 1% mismatch of two DNAs lowers the Tm 1.40° C. To determine the degree of identity required for two DNAs to hybridize under medium stringency conditions at 42° C., the following formula is used:

% Homology=100−[(Effective Tm−Hybridization Temperature)/1.4]

Applying this formula, the degree of identity required for two DNAs to hybridize under medium stringency conditions at 42° C. can be calculated.
Similar calculations can be made under other stringency conditions, as defined herein.
The present invention also relates to isolated polynucleotides obtained by (a) hybridizing a population of DNA under very low, low, medium, medium-high, high, or very high stringency conditions with any of (i) the genes of SEQ ID NOs: 2-4198, or subsequences thereof, or (ii) complementary strands thereof; and (b) isolating the hybridizing polynucleotide from the population of DNA. In a preferred aspect, the hybridizing polynucleotide encodes a polypeptide of any of SEQ ID NOs: 2-4198, or homologous polypeptides thereof.
In a third aspect, the present invention relates to isolated polypeptides having amino acid sequences which have a degree of identity to any of SEQ ID NOs: 4199-8395 of at least about 60%, preferably at least about 65%, more preferably at least about 70%, more preferably at least about 75%, more preferably at least about 80%, more preferably at least about 85%, even more preferably at least about 90%, most preferably at least about 95%, and even most preferably at least about 97%, which have biological activity (hereinafter “homologous polypeptides”). In a preferred aspect, the homologous polypeptides have an amino acid sequence which differs by ten amino acids, preferably by five amino acids, more preferably by four amino acids, even more preferably by three amino acids, most preferably by two amino acids, and even most preferably by one amino acid from the amino acid sequences of SEQ ID NOs: 4199-8395.
The polypeptides of the present invention preferably comprise the amino acid sequence of any of SEQ ID NOs: 4199-8395 or an allelic variant thereof; or a fragment thereof that has biological activity. In a more preferred aspect, the polypeptides of the present invention comprise the amino acid sequence of any of SEQ ID NOs: 4199-8395. In another preferred aspect, the polypeptides of the present invention comprise the mature polypeptide region of any of SEQ ID NOs: 4199-8395, or an allelic variant thereof; or a fragment thereof that has biological activity. In another preferred aspect, the polypeptides of the present invention comprise the mature polypeptide region of any of SEQ ID NOs: 4199-8395. In another preferred aspect, the polypeptides of the present invention consist of the amino acid sequence of any of SEQ ID NOs: 4199-8395 or an allelic variant thereof; or a fragment thereof that has biological activity. In another preferred aspect, the polypeptides of the present invention consist of the amino acid sequence of any of SEQ ID NOs: 4199-8395. In another preferred aspect, the polypeptides consist of the mature polypeptide region of any of SEQ ID NOs: 4199-8395 or an allelic variant thereof; or a fragment thereof that has biological activity. In another preferred aspect, the polypeptides consist of the mature polypeptide region of any of SEQ ID NOs: 4199-8395.
In a fourth aspect, the present invention relates to isolated substances having biological activity which are encoded by polynucleotides which hybridize, as described above, under very low stringency conditions, preferably low stringency conditions, more preferably medium stringency conditions, more preferably medium-high stringency conditions, even more preferably high stringency conditions, and most preferably very high stringency conditions with which hybridize under very low stringency conditions, preferably low stringency conditions, more preferably medium stringency conditions, more preferably medium-high stringency conditions, even more preferably high stringency conditions, and most preferably very high stringency conditions with any of (i) the genes of SEQ ID NOs: 2-4198, or subsequences thereof, or (ii) complementary strands thereof. A subsequence of any of SEQ ID NOs: 2-4198 may be at least 100 nucleotides or preferably at least 200 nucleotides. Moreover, the subsequence may encode a fragment, e.g., a polypeptide fragment, which has biological activity.
Nucleic Acid Constructs
The present invention also relates to nucleic acid constructs comprising an isolated gene or isolated genes (e.g., operon) of the present invention operably linked to one or more control sequences which direct the expression of the coding sequence in a suitable host cell under conditions compatible with the control sequences.
An isolated gene(s) of the present invention may be manipulated in a variety of ways to provide for production of a biologically active substance encoded directly or indirectly by the gene(s). Manipulation of the nucleotide sequence prior to its insertion into a vector may be desirable or necessary depending on the expression vector. The techniques for modifying nucleotide sequences utilizing recombinant DNA methods are well known in the art.
The control sequence may be an appropriate promoter sequence, a nucleotide sequence which is recognized by a host cell for expression of the gene(s) encoding the biologically active substance. The promoter sequence contains transcriptional control sequences which mediate the expression of the biologically active substance. The promoter may be any nucleotide sequence which shows transcriptional activity in the host cell of choice including mutant, truncated, and hybrid promoters, and may be obtained from genes encoding extracellular or intracellular polypeptides or biologically active substances either homologous or heterologous to the host cell.
Examples of suitable promoters for directing the transcription of the nucleic acid constructs of the present invention, especially in a bacterial host cell, are the promoters obtained from the E. coli lac operon, Streptomyces coelicolor agarase gene (dagA), Bacillus subtilis levansucrase gene (sacB), Bacillus licheniformis alpha-amylase qene (amyL), Bacillus stearothermophilus maltogenic amylase gene (amyM), Bacillus amyloliquefaciens alpha-amylase gene (amyQ), Bacillus licheniformis penicillinase gene (penP), Bacillus subtilis xylA and xylB genes, and prokaryotic beta-lactamase gene (Villa-Kamaroff et al., 1978, Proceedings of the National Academy of Sciences USA 75: 3727-3731), as well as the tac promoter (DeBoer et al., 1983, Proceedings of the National Academy of Sciences USA 80: 21-25). Further promoters are described in “Useful proteins from recombinant bacteria” in Scientific American, 1980, 242: 74-94; and in Sambrook et al., 1989, supra.
The control sequence may also be a suitable transcription terminator sequence, a sequence recognized by a host cell to terminate transcription. The terminator sequence is operably linked to the 3′ terminus of the gene encoding the biologically active substance. Any terminator which is functional in the host cell of choice may be used in the present invention.
The control sequence may also be a signal peptide coding region that codes for an amino acid sequence linked to the amino terminus of a polypeptide and directs the encoded polypeptide into the cell's secretory pathway. The 5′ end of the coding sequence of the nucleotide sequence may inherently contain a signal peptide coding region naturally linked in translation reading frame with the segment of the coding region which encodes the secreted polypeptide. Alternatively, the 5′ end of the coding sequence may contain a signal peptide coding region which is foreign to the coding sequence. The foreign signal peptide coding region may be required where the coding sequence does not naturally contain a signal peptide coding region. Alternatively, the foreign signal peptide coding region may simply replace the natural signal peptide coding region in order to enhance secretion of the polypeptide. However, any signal peptide coding region which directs the expressed polypeptide into the secretory pathway of a host cell of choice may be used in the present invention.
Effective signal peptide coding regions for bacterial host cells are the signal peptide coding regions obtained from the genes for Bacillus NCIB 11837 maltogenic amylase, Bacillus stearothermophilus alpha-amylase, Bacillus licheniformis subtilisin, Bacillus licheniformis beta-lactamase, Bacillus stearothermophilus neutral proteases (nprT, nprS, nprM), and Bacillus subtilis prsA. Further signal peptides are described by Simonen and Palva, 1993, Microbiological Reviews 57: 109-137.
The control sequence may also be a propeptide coding region that codes for an amino acid sequence positioned at the amino terminus of a polypeptide. The resultant polypeptide is known as a proenzyme or propolypeptide (or a zymogen in some cases). A propolypeptide is generally inactive and can be converted to a mature active polypeptide by catalytic or autocatalytic cleavage of the propeptide from the propolypeptide. The propeptide coding region may be obtained from the genes for Bacillus subtilis alkaline protease (aprE) and Bacillus subtilis neutral protease (nprT).
Where both signal peptide and propeptide regions are present at the amino terminus of a polypeptide, the propeptide region is positioned next to the amino terminus of a polypeptide and the signal peptide region is positioned next to the amino terminus of the propeptide region.
It may also be desirable to add regulatory sequences which allow the regulation of the expression of a biologically active substance relative to the growth of the host cell. Examples of regulatory systems are those which cause the expression of the gene to be turned on or off in response to a chemical or physical stimulus, including the presence of a regulatory compound. Regulatory systems in prokaryotic systems include the lac, tac, and trp operator systems. Other examples of regulatory sequences are those which allow for gene amplification. In eukaryotic systems, these include the dihydrofolate reductase gene which is amplified in the presence of methotrexate, and the metallothionein genes which are amplified with heavy metals. In these cases, the nucleotide sequence encoding the biologically active substance would be operably linked with the regulatory sequence.
Expression Vectors
The present invention also relates to recombinant expression vectors comprising an isolated gene of the present invention, a promoter, and transcriptional and translational stop signals. The various nucleic acid and control sequences described above may be joined together to produce a recombinant expression vector which may include one or more convenient restriction sites to allow for insertion or substitution of the nucleotide sequence encoding the polypeptide at such sites. Alternatively, a gene of the present invention may be expressed by inserting the nucleotide sequence or a nucleic acid construct comprising the sequence into an appropriate vector for expression. In creating the expression vector, the coding sequence is located in the vector so that the coding sequence is operably linked with the appropriate control sequences for expression.
The recombinant expression vector may be any vector (e.g., a plasmid or virus) which can be conveniently subjected to recombinant DNA procedures and can bring about the expression of a gene of the present invention. The choice of the vector will typically depend on the compatibility of the vector with the host cell into which the vector is to be introduced. The vectors may be linear or closed circular plasmids.
The vector may be an autonomously replicating vector, i.e., a vector which exists as an extrachromosomal entity, the replication of which is independent of chromosomal replication, e.g., a plasmid, an extrachromosomal element, a minichromosome, or an artificial chromosome. The vector may contain any means for assuring self-replication. Alternatively, the vector may be one which, when introduced into the host cell, is integrated into the genome and replicated together with the chromosome(s) into which it has been integrated. Furthermore, a single vector or plasmid or two or more vectors or plasmids which together contain the total DNA to be introduced into the genome of the host cell, or a transposon may be used.
The vectors of the present invention preferably contain one or more selectable markers which permit easy selection of transformed cells. A selectable marker is a gene the product of which provides for biocide or viral resistance, resistance to heavy metals, prototrophy to auxotrophs, and the like.
Examples of bacterial selectable markers are the dal genes from Bacillus subtilis or Bacillus licheniformis, or markers which confer antibiotic resistance such as ampicillin, kanamycin, chloramphenicol or tetracycline resistance.
The vectors of the present invention preferably contain an element(s) that permits integration of the vector into the host cell's genome or autonomous replication of the vector in the cell independent of the genome.
For integration into the host cell genome, the vector may rely on portions of the sequence of the gene or any other element of the vector for integration of the vector into the genome by homologous or nonhomologous recombination. Alternatively, the vector may contain additional nucleotide sequences for directing integration by homologous recombination into the genome of the host cell. The additional nucleotide sequences enable the vector to be integrated into the host cell genome at a precise location(s) in the chromosome(s). To increase the likelihood of integration at a precise location, the integrational elements should preferably contain a sufficient number of nucleotides, such as 100 to 10,000 base pairs, preferably 400 to 10,000 base pairs, and most preferably 800 to 10,000 base pairs, which are highly homologous with the corresponding target sequence to enhance the probability of homologous recombination. The integrational elements may be any sequence that is homologous with the target sequence in the genome of the host cell. Furthermore, the integrational elements may be non-encoding or encoding nucleotide sequences. On the other hand, the vector may be integrated into the genome of the host cell by non-homologous recombination.
For autonomous replication, the vector may further comprise an origin of replication enabling the vector to replicate autonomously in the host cell in question. The origin of replication may be any plasmid replicator mediating autonomous replication which functions in a cell. The term “origin of replication” or “plasmid replicator” is defined herein as a sequence that enables a plasmid or vector to replicate in vivo. Examples of bacterial origins of replication are the origins of replication of plasmids pBR322, pUC19, pACYC177, and pACYC184 permitting replication in E. coli, and pUB110, pE194, pTA1060, and pAMβ1 permitting replication in Bacillus. 
More than one copy of a gene of the present invention may be inserted into the host cell to increase production of the gene product. An increase in the copy number of the gene can be obtained by integrating at least one additional copy of the sequence into the host cell genome or by including an amplifiable selectable marker gene with a gene of the present invention where cells containing amplified copies of the selectable marker gene, and thereby additional copies of the gene of the present invention, can be selected for by cultivating the cells in the presence of the appropriate selectable agent.
The procedures used to ligate the elements described above to construct the recombinant expression vectors of the present invention are well known to one skilled in the art (see, e.g., Sambrook et al., 1989, supra).
Host Cells
The present invention also relates to recombinant host cells, comprising an isolated gene of the present invention, where the host cells are advantageously used in the recombinant production of a biologically active substance encoded by the gene. A vector comprising a gene of the present invention is introduced into a host cell so that the vector is maintained as a chromosomal integrant or as a self-replicating extra-chromosomal vector as described earlier. The term “host cell” encompasses any progeny of a parent cell that is not identical to the parent cell due to mutations that occur during replication. The choice of a host cell will to a large extent depend upon the gene encoding the biologically active substance and its source.
The host cell may be any unicellular microorganism, e.g., a prokaryote.
Useful unicellular cells are bacterial cells such as gram positive bacteria including, but not limited to, a Bacillus cell, e.g., Bacillus alkalophilus, Bacillus amyloliquefaciens, Bacillus brevis, Bacillus cereus, Bacillus circulans, Bacillus clausii, Bacillus coagulans, Bacillus fastidiosus, Bacillus firmus, Bacillus lautus, Bacillus lentus, Bacillus licheniformis, Bacillus macerans, Bacillus megaterium, Bacillus methanolicus, Bacillus pumilus, Bacillus sphaericus, Bacillus stearothermophilus, Bacillus subtilis, and Bacillus thuringiensis; or a Streptomyces cell, e.g., Streptomyces lividans and Streptomyces murinus, or gram negative bacteria such as E. coli and Pseudomonas sp. In a preferred aspect, the bacterial host cell is a Bacillus lentus, Bacillus licheniformis, Bacillus stearothermophilus, or Bacillus subtilis cell. In another preferred aspect, the Bacillus cell is an alkalophilic Bacillus. 
The introduction of a vector into a bacterial host cell may, for instance, be effected by protoplast transformation (see, e.g., Chang and Cohen, 1979, Molecular General Genetics 168: 111-115), using competent cells (see, e.g., Young and Spizizen, 1961, Journal of Bacteriology 81: 823-829, or Dubnau and Davidoff-Abelson, 1971, Journal of Molecular Biology 56: 209-221), electroporation (see, e.g., Shigekawa and Dower, 1988, Biotechniques 6: 742-751), or conjugation (see, e.g., Koehler and Thorne, 1987, Journal of Bacteriology 169: 5771-5278).
Methods of Production
The present invention also relates to methods for producing a biologically active substance of the present invention comprising (a) cultivating a strain, which in its wild-type form is capable of producing the biologically active substance, under conditions conducive for production of the biologically active substance; and (b) recovering the biologically active substance. Preferably, the strain is of the genus Bacillus, and more preferably Bacillus licheniformis. 
The present invention also relates to methods for producing a biologically active substance of the present invention comprising (a) cultivating a host cell under conditions conducive for production of the biologically active substance; and (b) recovering the biologically active substance.
The present invention also relates to methods for producing a biologically active substance of the present invention comprising (a) cultivating a host cell under conditions conducive for production of the biologically active substance, wherein the host cell comprises a mutant polynucleotide comprising at least one mutation in the coding region of any of SEQ ID NOs: 2-4198, wherein the mutant polynucleotide encodes a biologically active substance which consists of SEQ ID NOs: 4199-8395, respectively, and (b) recovering the biologically active substance.
In the production methods of the present invention, the cells are cultivated in a nutrient medium suitable for production of the biologically active substance using methods known in the art. For example, the cell may be cultivated by shake flask cultivation, and small-scale or large-scale fermentation (including continuous, batch, fed-batch, or solid state fermentations) in laboratory or industrial fermentors performed in a suitable medium and under conditions allowing the biologically active substance to be expressed and/or isolated. The cultivation takes place in a suitable nutrient medium comprising carbon and nitrogen sources and inorganic salts, using procedures known in the art. Suitable media are available from commercial suppliers or may be prepared according to published compositions (e.g., in catalogues of the American Type Culture Collection). If the biologically active substance is secreted into the nutrient medium, the biologically active substance can be recovered directly from the medium. If the biologically active substance is not secreted, it can be recovered from cell lysates.
The biologically active substances may be detected using methods known in the art that are specific for the polypeptides. These detection methods may include use of specific antibodies, formation of an enzyme product, or disappearance of an enzyme substrate. For example, an enzyme assay may be used to determine the activity of an enzyme.
The resulting biologically active substances may be recovered by methods known in the art. For example, the biologically active substances may be recovered from the nutrient medium by conventional procedures including, but not limited to, centrifugation, filtration, extraction, spray-drying, evaporation, or precipitation.
The biologically active substances of the present invention may be purified by a variety of procedures known in the art including, but not limited to, chromatography (e.g., ion exchange, affinity, hydrophobic, chromatofocusing, and size exclusion), electrophoretic procedures (e.g., preparative isoelectric focusing), differential solubility (e.g., ammonium sulfate precipitation), SDS-PAGE, or extraction (see, e.g., Protein Purification, J.-C. Janson and Lars Ryden, editors, VCH Publishers, New York, 1989).
Plants
The present invention also relates to a transgenic plant, plant part, or plant cell which has been transformed with a gene encoding a biologically active substance of the present invention so as to express and produce the biologically active substance in recoverable quantities. The biologically active substance may be recovered from the plant or plant part. Alternatively, the plant or plant part containing the recombinant biologically active substance may be used as such for improving the quality of a food or feed, e.g., improving nutritional value, palatability, and rheological properties, or to destroy an antinutritive factor.
The transgenic plant can be dicotyledonous (a dicot) or monocotyledonous (a monocot). Examples of monocot plants are grasses, such as meadow grass (blue grass, Poa), forage grass such as festuca, lolium, temperate grass, such as Agrostis, and cereals, e.g., wheat, oats, rye, barley, rice, sorghum, and maize (corn).
Examples of dicot plants are tobacco, legumes, such as lupins, potato, sugar beet, pea, bean and soybean, and cruciferous plants (family Brassicaceae), such as cauliflower, rape seed, and the closely related model organism Arabidopsis thaliana. 
Examples of plant parts are stem, callus, leaves, root, fruits, seeds, and tubers as well as the individual tissues comprising these parts, e.g., epidermis, mesophyll, parenchyme, vascular tissues, meristems. In the present context, also specific plant cell compartments, such as chloroplast, apoplast, mitochondria, vacuole, peroxisomes and cytoplasm are considered to be a plant part. Furthermore, any plant cell, whatever the tissue origin, is considered to be a plant part. Likewise, plant parts such as specific tissues and cells isolated to facilitate the utilisation of the invention are also considered plant parts e.g. embryos, endosperms, aleurone and seeds coats.
Also included within the scope of the present invention are the progeny of such plants, plant parts, and plant cells.
The transgenic plant or plant cell expressing a biologically active substance of the present invention may be constructed in accordance with methods known in the art. Briefly, the plant or plant cell is constructed by incorporating one or more expression constructs encoding a biologically active substance of the present invention into the plant host genome and propagating the resulting modified plant or plant cell into a transgenic plant or plant cell.
The expression construct is conveniently a nucleic acid construct which comprises a gene encoding a biologically active substance of the present invention operably linked with appropriate regulatory sequences required for expression of the nucleotide sequence in the plant or plant part of choice. Furthermore, the expression construct may comprise a selectable marker useful for identifying host cells into which the expression construct has been integrated and DNA sequences necessary for introduction of the construct into the plant in question (the latter depends on the DNA introduction method to be used).
The choice of regulatory sequences, such as promoter and terminator sequences and optionally signal or transit sequences is determined, for example, on the basis of when, where, and how the biologically active substance is desired to be expressed. For instance, the expression of the gene encoding a biologically active substance of the present invention may be constitutive or inducible, or may be developmental, stage or tissue specific, and the gene product may be targeted to a specific tissue or plant part such as seeds or leaves. Regulatory sequences are, for example, described by Tague et al., 1988, Plant Physiology 86: 506.
For constitutive expression the 35S-CaMV, the maize ubiquitin 1 and the rice actin 1 promoter may be used (Franck et al., 1980. Cell 21: 285-294, Christensen A H, Sharrock R A and Quail, 1992, Plant Mo. Biol. 18: 675-689.; Zhang W, McElroy D. and Wu R., 1991, Plant Cell 3: 1155-1165). Organ-specific promoters may be, for example, a promoter from storage sink tissues such as seeds, potato tubers, and fruits (Edwards and Coruzzi, 1990, Ann. Rev. Genet. 24: 275-303), or from metabolic sink tissues such as meristems (Ito et al., 1994, Plant Mol. Biol. 24: 863-878), a seed specific promoter such as the glutelin, prolamin, globulin, or albumin promoter from rice (Wu et al., 1998, Plant and Cell Physiology 39: 885-889), a Vicia faba promoter from the legumin B4 and the unknown seed protein gene from Vicia faba (Conrad et al., 1998, Journal of Plant Physiology 152: 708-711), a promoter from a seed oil body protein (Chen et al., 1998, Plant and Cell Physiology 39: 935-941), the storage protein napA promoter from Brassica napus, or any other seed specific promoter known in the art, e.g., as described in WO 91/14772. Furthermore, the promoter may be a leaf specific promoter such as the rbcs promoter from rice or tomato (Kyozuka et al., 1993, Plant Physiology 102: 991-1000, the chlorella virus adenine methyltransferase gene promoter (Mitra and Higgins, 1994, Plant Molecular Biology 26: 85-93), or the aldP gene promoter from rice (Kagaya et al., 1995, Molecular and General Genetics 248: 668-674), or a wound inducible promoter such as the potato pin2 promoter (Xu et al., 1993, Plant Molecular Biology 22: 573-588). Likewise, the promoter may inducible by abiotic treatments such as temperature, drought or alterations in salinity or induced by exogenously applied substances that activate the promoter, e.g., ethanol, oestrogens, plant hormones like ethylene, abscisic acid and gibberellic acid and heavy metals.
A promoter enhancer element may also be used to achieve higher expression of the enzyme in the plant. For instance, the promoter enhancer element may be an intron which is placed between the promoter and the nucleotide sequence encoding a biologically active substance of the present invention. For instance, Xu et al., 1993, supra disclose the use of the first intron of the rice actin 1 gene to enhance expression.
The selectable marker gene and any other parts of the expression construct may be chosen from those available in the art.
The nucleic acid construct is incorporated into the plant genome according to conventional techniques known in the art, including Agrobacterium-mediated transformation, virus-mediated transformation, microinjection, particle bombardment, biolistic transformation, and electroporation (Gasser et al, 1990, Science 244: 1293; Potrykus, 1990, Bio/Technology 8: 535; Shimamoto et al., 1989, Nature 338: 274).
Presently, Agrobacterium tumefaciens-mediated gene transfer is the method of choice for generating transgenic dicots (for a review, see Hooykas and Schilperoort, 1992, Plant Molecular Biology 19: 15-38). However it can also be used for transforming monocots, although other transformation methods are generally preferred for these plants. Presently, the method of choice for generating transgenic monocots is particle bombardment (microscopic gold or tungsten particles coated with the transforming DNA) of embryonic calli or developing embryos (Christou, 1992, Plant Journal 2: 275-281; Shimamoto, 1994, Current Opinion Biotechnology 5: 158-162; Vasil et al., 1992, Bio/Technology 10: 667-674). An alternative method for transformation of monocots is based on protoplast transformation as described by Omirulleh et al., 1993, Plant Molecular Biology 21: 415-428.
Following transformation, the transformants having incorporated therein the expression construct are selected and regenerated into whole plants according to methods well-known in the art. Often the transformation procedure is designed for the selective elimination of selection genes either during regeneration or in the following generations by using, for example, co-transformation with two separate T-DNA constructs or site specific excision of the selection gene by a specific recombinase.
The present invention also relates to methods for producing a biologically active substance of the present invention comprising (a) cultivating a transgenic plant or a plant cell comprising a gene encoding a biologically active substance of the present invention under conditions conducive for production of the biologically active substance; and (b) recovering the biologically active substance.
Removal or Reduction of Biologically Active Substance
The present invention also relates to methods for producing a mutant of a parent cell, which comprises disrupting or deleting all or a portion of a gene encoding a biologically active substance of the present invention, which results in the mutant cell producing less of the biologically active substance than the parent cell when cultivated under the same conditions.
The mutant cell may be constructed by reducing or eliminating expression of a gene encoding or regulatory synthesis of a biologically active substance of the present invention using methods well known in the art, for example, insertions, disruptions, replacements, or deletions. The gene to be modified or inactivated may be, for example, the coding region or a part thereof essential for activity, or a regulatory element of the gene required for the expression of the coding region. An example of such a regulatory or control sequence may be a promoter sequence or a functional part thereof, i.e., a part that is sufficient for affecting expression of the gene. Other control sequences for possible modification include, but are not limited to, a leader, propeptide sequence, signal peptide sequence, transcription terminator, and transcriptional activator.
Modification or inactivation of the gene may be performed by subjecting the parent cell to mutagenesis and selecting for mutant cells in which expression of the gene has been reduced or eliminated. The mutagenesis, which may be specific or random, may be performed, for example, by use of a suitable physical or chemical mutagenizing agent, by use of a suitable oligonucleotide, or by subjecting the DNA sequence to PCR generated mutagenesis. Furthermore, the mutagenesis may be performed by use of any combination of these mutagenizing agents.
Examples of a physical or chemical mutagenizing agent suitable for the present purpose include ultraviolet (UV) irradiation, hydroxylamine, N-methyl-N′-nitro-N-nitrosoguanidine (MNNG), O-methyl hydroxylamine, nitrous acid, ethyl methane sulphonate (EMS), sodium bisulphite, formic acid, and nucleotide analogues.
When such agents are used, the mutagenesis is typically performed by incubating the parent cell to be mutagenized in the presence of the mutagenizing agent of choice under suitable conditions, and screening and/or selecting for mutant cells exhibiting reduced or no expression of the gene.
Modification or inactivation of the nucleotide sequence may be accomplished by introduction, substitution, or removal of one or more nucleotides in the gene or a regulatory element required for the transcription or translation thereof. For example, nucleotides may be inserted or removed so as to result in the introduction of a stop codon, the removal of the start codon, or a change in the open reading frame. Such modification or inactivation may be accomplished by site-directed mutagenesis or PCR generated mutagenesis in accordance with methods known in the art. Although, in principle, the modification may be performed in vivo, i.e., directly on the cell expressing the nucleotide sequence to be modified, it is preferred that the modification be performed in vitro as exemplified below.
An example of a convenient way to eliminate or reduce expression of a nucleotide sequence by a cell of choice is based on techniques of gene replacement, gene deletion, or gene disruption. For example, in the gene disruption method, a nucleic acid sequence corresponding to the endogenous nucleotide sequence is mutagenized in vitro to produce a defective nucleic acid sequence which is then transformed into the parent cell to produce a defective gene. By homologous recombination, the defective nucleic acid sequence replaces the endogenous nucleotide sequence. It may be desirable that the defective nucleotide sequence also encodes a marker that may be used for selection of transformants in which the nucleotide sequence has been modified or destroyed. In a particularly preferred aspect, the nucleotide sequence is disrupted with a selectable marker such as those described herein.
Alternatively, modification or inactivation of the nucleotide sequence may be performed by established anti-sense techniques using a sequence complementary to the nucleotide sequence. More specifically, expression of the nucleotide sequence by a cell may be reduced or eliminated by introducing a sequence complementary to the nucleic acid sequence of the gene that may be transcribed in the cell and is capable of hybridizing to the mRNA produced in the cell. Under conditions allowing the complementary anti-sense nucleotide sequence to hybridize to the mRNA, the amount of protein translated is thus reduced or eliminated.
The present invention further relates to a mutant cell of a parent cell which comprises a disruption or deletion of a nucleotide sequence encoding the biologically active substance or a control sequence thereof, which results in the mutant cell producing less of the biologically active substance than the parent cell.
The biologically active substance-deficient mutant cells so created are particularly useful as host cells for the expression of homologous and/or heterologous substances, such as polypeptides. Therefore, the present invention further relates to methods for producing a homologous or heterologous substance comprising (a) cultivating the mutant cell under conditions conducive for production of the substance; and (b) recovering the substance. The term “heterologous substances” is defined herein as substances which are not native to the host cell, a native substance in which modifications have been made to alter the native sequence, or a native substance whose expression is quantitatively altered as a result of a manipulation of the host cell by recombinant DNA techniques.
In a further aspect, the present invention relates to a method for producing a protein product essentially free of a biologically active substance by fermentation of a cell which produces both a biologically active substance of the present invention as well as the protein product of interest by adding an effective amount of an agent capable of inhibiting activity of the biologically active substance to the fermentation broth before, during, or after the fermentation has been completed, recovering the product of interest from the fermentation broth, and optionally subjecting the recovered product to further purification.
In accordance with this aspect of the invention, it is possible to remove at least 60%, preferably at least 75%, more preferably at least 85%, still more preferably at least 95%, and most preferably at least 99% of the biologically active substance. Complete removal of biologically active substance may be obtained by use of this method.
The methods used for cultivation and purification of the product of interest may be performed by methods known in the art.
The methods of the present invention for producing an essentially biologically active substance-free product is of particular interest in the production of prokaryotic polypeptides, in particular bacterial proteins such as enzymes. The enzyme may be selected from, e.g., an amylolytic enzyme, lipolytic enzyme, proteolytic enzyme, cellulytic enzyme, oxidoreductase, or plant cell-wall degrading enzyme. Examples of such enzymes include an aminopeptidase, amylase, amyloglucosidase, carbohydrase, carboxypeptidase, catalase, cellulase, chitinase, cutinase, cyclodextrin glycosyltransferase, deoxyribonuclease, esterase, galactosidase, beta-galactosidase, glucoamylase, glucose oxidase, glucosidase, haloperoxidase, hemicellulase, invertase, isomerase, laccase, ligase, lipase, lyase, mannosidase, oxidase, pectinolytic enzyme, peroxidase, phytase, phenoloxidase, polyphenoloxidase, proteolytic enzyme, ribonuclease, transferase, transglutaminase, or xylanase. The biologically active substance-deficient cells may also be used to express heterologous proteins of pharmaceutical interest such as hormones, growth factors, receptors, and the like.
It will be understood that the term “prokaryotic polypeptides” includes not only native polypeptides, but also those polypeptides, e.g., enzymes, which have been modified by amino acid substitutions, deletions or additions, or other such modifications to enhance activity, thermostability, pH tolerance and the like.
In a further aspect, the present invention relates to a product of a protein or substance essentially free of a biologically active substance of the invention, produced by a method of the present invention.
Compositions
The present invention also relates to compositions comprising a biologically active substance of the present invention. Preferably, the compositions are enriched in the biologically active substance. The term “enriched” indicates that the biologically active substance of the composition has been increased, e.g., with an enrichment factor of 1.1.
The composition may comprise a biologically active substance of the invention as the major component, e.g., a mono-component composition. Alternatively, the composition may comprise multiple biologically active substances, for example, multiple enzymes, such as an aminopeptidase, amylase, carbohydrase, carboxypeptidase, catalase, cellulase, chitinase, cutinase, cyclodextrin glycosyltransferase, deoxyribonuclease, esterase, alpha-galactosidase, beta-galactosidase, glucoamylase, alpha-glucosidase, beta-glucosidase, haloperoxidase, invertase, laccase, lipase, mannosidase, oxidase, pectinolytic enzyme, peptidoglutaminase, peroxidase, phytase, polyphenoloxidase, proteolytic enzyme, ribonuclease, transglutaminase, or xylanase.
The compositions may be prepared in accordance with methods known in the art and may be in the form of a liquid or a dry composition. For instance, the composition may be in the form of a granulate or a microgranulate. The biologically active substance to be included in the composition may be stabilized in accordance with methods known in the art.
Methods for Using the Bacillus licheniformis Chromosome
The present invention also relates to methods for using the Bacillus licheniformis chromosome.
The chromosome of Bacillus licheniformis serves as a reservoir of new genes/proteins that have likely environmental, energy, health, and industrial applications (e.g., enzymes, antibiotics, biochemicals). A clear extension of this is that the newly discovered molecules can be used as starting points for further improvements via well-established gene shuffling, directed evolution, and protein engineering methods. Additionally, regions or motifs (e.g., signal peptides, active sites, substrate-binding regions) from the newly discovered molecules may be employed to derive novel chimeras with industrially advantageous properties.
The genes encoded in the chromosome may be used for monitoring global gene expression during the life cycle of the organism or during industrial fermentations (e.g., implemented on DNA microarrays). By monitoring global gene expression, for example, improved processes for industrial fermentation can be implemented with greater efficiency and economy.
The chromosome is useful in comparative evolutionary and ecological studies. For example, dozens of Bacillus licheniformis isolates can be readily compared on a global scale by hybridization of their genomic DNAs to a microarray fabricated from the reference strain presented in this case (so-called comparative genomic hybridization). Using this method, one can compare various isolates to look for similarities/differences among geographical and environmental niches or among biocontrol strains versus saprophytic isolates.
The chromosome sequence may be used to construct the metabolic blueprint for Bacillus licheniformis that includes all catabolic and anabolic pathways, signaling pathways, regulatory networks, growth substrates, biochemical intermediates, end products, electron donors/acceptors and others. In doing so, it is possible to modify the metabolic machinery of the organism by deleting unwanted pathways and/or adding enzymes/pathways from other organisms to generate useful chemicals and intermediates.
The pathways and components that contribute to production of extracellular and surface proteins in Bacillus licheniformis can be extracted from the chromosomal sequence. This affords opportunities for improved production of extracellular proteins by genetic manipulation of the secretion machinery.
The chromosome data allows deduction of the essential genes for Bacillus licheniformis (either by comparison to related bacteria such as Bacillus subtilis or by systematic gene-by-gene knock outs). Thus it has become possible to design custom-made strains which contain only the genes that are essential for production of specific proteins or metabolites (so-called cell factory concept).
The chromosome data may be used to construct interspecies hybrids between Bacillus licheniformis and other bacteria. Venter et al., 2003, Proc. Nat. Acad. Sci. USA 100, 15440-15445 have shown that it is possible to construct an entire virus genome from smaller DNA segments. Thus, segments of the Bacillus licheniformis chromosome may be employed to derive novel chromosomal segments or even entire chimeric chromosomes for specific applications.
In a preferred aspect, methods for using the Bacillus licheniformis chromosome include host improvement, e.g., secretion of a protein or metabolite, genome shuffling, construction of new genomes, metabolic engineering and pathway reconstruction, carrier for heterologous expression vectors, microarrays as described herein, identification of polypeptides in proteomics analyses, and comparative genomics with other Bacillus species or related organisms.
Methods for Isolating Genes
The present invention also relates to methods for isolating a gene encoding a biologically active substance from a microbial strain. The method comprises first the addition of a mixture of first labeled nucleic acid probes, isolated from a microbial strain cultured on medium without an inducing substrate, and a mixture of second labeled nucleic acid probes, isolated from the microbial strain cultured on medium with the inducing substrate, to an array of Bacillus licheniformis genes selected from the group consisting of nucleotides SEQ ID NOs: 2-4198, complementary strands of SEQ ID NOs: 2-4198, or fragments of SEQ ID NOs: 2-4198, under conditions where the labeled nucleic acid probes hybridize to complementary sequences of the Bacillus licheniformis genes on the array. The first nucleic acid probes are labeled with a first reporter and the second nucleic acid probes are labeled with a second reporter. The array is then examined under conditions wherein the relative expression of the genes of the microbial strain is determined by the observed hybridization reporter signal of each spot on the array in which (i) the Bacillus licheniformis genes on the array that hybridize to the first nucleic acid probes produce a distinct first hybridization reporter signal or to the second nucleic acid probes produce a distinct second hybridization reporter signal, and (ii) the Bacillus licheniformis genes on the array that hybridize to both the first and second nucleic acid probes produce a distinct combined hybridization reporter signal. The probe is then sequenced to isolate from the microbial strain the corresponding gene that encodes an enzyme that degrades or converts the substrate.
Enzymes. The gene of interest may encode any enzyme including an oxidoreductase, transferase, hydrolase, lyase, isomerase, or ligase. In a preferred aspect, the enzyme is an acylase, alpha-glucosidase, amidase, aminopeptidase, amylase, carbohydrase, carboxypeptidase, catalase, cellulase, chitinase, cutinase, cyclodextrin glycosyltransferase, deoxyribonuclease, dextrinase, endoglucanase, esterase, galactanase, alpha-galactosidase, beta-galactosidase, glucoamylase, glucanase, glucocerebrosidase, alpha-glucosidase, beta-glucosidase, hemicellulase, invertase, laccase, lignase, lipase, lysin, mannosidase, mutanase, oxidase, pectinolytic enzyme, peroxidase, phosphatase, phospholipase, phytase, polyphenoloxidase, proteolytic enzyme, pullulanase, ribonuclease, transglutaminase, urokinase, or xylanase.
Inducing Substrate. The inducing substrate may be any substrate that is subject to the action of an enzyme, i.e., that degrades or converts the substrate. In a preferred aspect, the inducing substrate is lignin or a lignin-containing material. In a more preferred aspect, the lignin-containing material is lignocellulose. In another preferred aspect, the inducing substrate is cellulose. In another preferred aspect, the inducing substrate is hemicellulose. In another preferred aspect, the inducing substrate is pectin. In another preferred aspect, the inducing substrate is a lipid. In another preferred aspect, the inducing substrate is phospholipid. In another preferred aspect, the inducing substrate is phytic acid. In another preferred aspect, the inducing substrate is protein. In another preferred aspect, the inducing substrate is a starch. In another preferred aspect, the inducing substrate is a medium that is low in nutrients such as amino acids, carbon, nitrogen, phosphate, or iron.
In a more preferred aspect, the protein substrate is blood, casein, egg, gelatin, gluten, milk protein, or soy protein. In another more preferred aspect, the lignin-containing material is hardwood thermomechanical pulp. In another more preferred aspect, the lignocellulose is corn stover. In another more preferred aspect, the lignocellulose is white poplar. In another more preferred aspect, the lignocellulose is rice straw. In another more preferred aspect, the lignocellulose is switch grass.
Microbial Strains. In the methods of the present invention, the microbial strain may be any microbial strain. The strain is cultured on a suitable nutrient medium with and without a substrate of interest. The strain cultured on medium without the substrate is used as a reference for identifying differences in expression of the same or similar complement of genes in the strain cultured on medium with substrate. The strain may be a wild-type, mutant, or recombinant strain.
In the methods of the present invention, the microbial strain is preferably a bacterium. In a more preferred aspect, the bacterium is a Bacillus, Pseudomonas, or Streptomyces strain or E. coli. 
The Bacillus strain may be any Bacillus strain. In a preferred aspect, the Bacillus strain is Bacillus alkalophilus, Bacillus amyloliquefaciens, Bacillus brevis, Bacillus cereus, Bacillus circulans, Bacillus clausii, Bacillus coagulans, Bacillus fastidiosus, Bacillus firmus, Bacillus lautus, Bacillus lentus, Bacillus licheniformis, Bacillus macerans, Bacillus megaterium, Bacillus methanolicus, Bacillus pumilus, Bacillus sphaericus, Bacillus stearothermophilus, Bacillus subtilis, or Bacillus thuringiensis. It will be understood that the term “Bacillus” also encompasses relatives of Bacillus such as Paenibacillus, Oceanobacillus, and the like.
The Pseudomonas strain may be any Pseudomonas strain. In a preferred aspect, the Pseudomonas strain is Pseudomonas acidovorans, Pseudomonas aeruginosa, Pseudomonas alcaligenes, Pseudomonas anguilliseptica, Pseudomonas abtimicrobica, Pseudomonas aurantiaca, Pseudomonas aureofaciens, Pseudomonas beijerinckii, Pseudomonas boreopolis, Pseudomonas chlororaphis, Pseudomonas citronellolis, Pseudomonas cocovenenans, Pseudomonas diminuta, Pseudomonas doudoroffii, Pseudomonas echinoides, Pseudomonas elongata, Pseudomonas fluorescens, Pseudomonas fragi, Pseudomonas halophobica, Pseudomonas huttiensis, Pseudomonas indigofera, Pseudomonas lanceolata, Pseudomonas lemoignei, Pseudomonas lundensis, Pseudomonas mendocina, Pseudomonas mephitica, Pseudomonas mucidolens, Pseudomonas oleovorans, Pseudomonas phenazinium, Pseudomonas pictorium, Pseudomonas putida, Pseudomonas resinovorans, Pseudomonas saccharophila, Pseudomonas stanieri, Pseudomonas stutzeri, Pseudomonas taetrolens, or Pseudomonas vesicularis. 
The Streptomyces strain may be any Streptomyces strain. In a preferred aspect, the Streptomyces strain is Streptomyces lividans. In another preferred aspect, the Streptomyces strain is Streptomyces murinus. 
Microarrays. The term “an array of Bacillus licheniformis genes” is defined herein as a linear or two-dimensional array of preferably discrete elements of an array of Bacillus licheniformis genes selected from the group consisting of nucleotides SEQ ID NOs: 2-4198, complementary strands of SEQ ID NOs: 2-4198, or fragments of SEQ ID NOs: 2-4198 (e.g., synthetic oligonucleotides of, for example, 40-60 nucleotides), wherein each discrete element has a finite area, formed on the surface of a solid support. It shall be understood that the term “Bacillus licheniformis genes” encompasses nucleotides SEQ ID NOs: 2-4198, complementary strands of SEQ ID NOs: 2-4198, or fragments of SEQ ID NOs: 2-4198.
The term “microarray” is defined herein as an array of Bacillus licheniformis gene elements having a density of discrete of Bacillus licheniformis gene elements of at least about 100/cm2, and preferably at least about 1000/cm2. The Bacillus licheniformis gene elements in a microarray have typical dimensions, e.g., diameters, in the range of between about 10 to about 250 μm, preferably in the range of between about 10 to about 200 μm, more preferably in the range of between about 20 to about 150 μm, even more preferably in the range of between about 20 to about 100 μm, most preferably in the range of between about 50 to about 100 μm, and even most preferably in the range of between about 80 to about 100 μm, and are separated from other gene elements in the microarray by about the same distance.
Methods and instruments for forming microarrays on the surface of a solid support are well known in the art. See, for example, U.S. Pat. Nos. 5,807,522; 5,700,637; and 5,770,151. The instrument may be an automated device such as described in U.S. Pat. No. 5,807,522.
The term “a substrate containing an array of Bacillus licheniformis genes” is defined herein as a solid support having deposited on the surface of the support one or more of a plurality of Bacillus licheniformis genes, as described herein, for use in detecting binding of labeled nucleic acids to the Bacillus licheniformis genes.
The substrate may, in one aspect, be a glass support (e.g., glass slide) having a hydrophilic or hydrophobic coating on the surface of the support, and an array of distinct random nucleic acid fragments bound to the coating, where each distinct random nucleic acid fragment is disposed at a separate, defined position.
Each microarray in the substrate preferably contains at least 103 distinct Bacillus licheniformis in a surface area of less than about 5 or 6 cm2. Each distinct Bacillus licheniformis gene (i) is disposed at a separate, defined position on the array, (ii) has a length of at least 50 bp, and (iii) is present in a defined amount between about 0.1 femtomoles and 100 nanomoles or higher if necessary.
For a hydrophilic coating, the glass slide is coated by placing a film of a polycationic polymer with a uniform thickness on the surface of the slide and drying the film to form a dried coating. The amount of polycationic polymer added should be sufficient to form at least a monolayer of polymers on the glass surface. The polymer film is bound to the surface via electrostatic binding between negative silyl-OH groups on the surface and charged cationic groups in the polymers. Such polycationic polymers include, but are not limited to, polylysine and polyarginine.
Another coating strategy employs reactive aldehydes to couple DNA to the slides (Schena et al., 1996, Proceedings of the National Academy of Science USA 93: 10614-10619; Heller at al., 1997, Proceedings of the National Academy of Science USA 94: 2150-2155).
Alternatively, the surface may have a relatively hydrophobic character, i.e., one that causes aqueous medium deposited on the surface to bead. A variety of known hydrophobic polymers, such as polystyrene, polypropylene, or polyethylene, have desirable hydrophobic properties, as do glass and a variety of lubricant or other hydrophobic films that may be applied to the support surface. A support surface is “hydrophobic” if an aqueous droplet applied to the surface does not spread out substantially beyond the area size of the applied droplet, wherein the surface acts to prevent spreading of the droplet applied to the surface by hydrophobic interaction with the droplet.
In another aspect, the substrate may be a multi-cell substrate where each cell contains a microarray of Bacillus licheniformis and preferably an identical microarray, formed on a porous surface. For example, a 96-cell array may typically have array dimensions between about 12 and 244 mm in width and 8 and 400 mm in length, with the cells in the array having width and length dimension of 1/12 and 1⁄8 the array width and length dimensions, respectively, i.e., between about 1 and 20 in width and 1 and 50 mm in length.
The solid support may include a water-impermeable backing such as a glass slide or rigid polymer sheet, or other non-porous material. Formed on the surface of the backing is a water-permeable film, which is formed of porous material. Such porous materials include, but are not limited to, nitrocellulose membrane nylon, polypropylene, and polyvinylidene difluoride (PVDF) polymer. The thickness of the film is preferably between about 10 and 1000 μm. The film may be applied to the backing by spraying or coating, or by applying a preformed membrane to the backing.
Alternatively, the solid support may be simply a filter composed of nitrocellulose, nylon, polypropylene, or polyvinylidene difluoride (PVDF) polymer, or, for that matter, any material suitable for use.
The film surface may be partitioned into a desirable array of cells by water-impermeable grid lines typically at a distance of about 100 to 2000 μm above the film surface. The grid lines can be formed on the surface of the film by laying down an uncured flowable resin or elastomer solution in an array grid, allowing the material to infiltrate the porous film down to the backing, and then curing the grid lines to form the cell-array substrate.
The barrier material of the grid lines may be a flowable silicone, wax-based material, thermoset material (e.g., epoxy), or any other useful material. The grid lines may be applied to the solid support using a narrow syringe, printing techniques, heat-seal stamping, or any other useful method known in the art.
Each well preferably contains a microarray of distinct Bacillus licheniformis genes. “Distinct Bacillus licheniformis genes” as applied to the genes forming a microarray is defined herein as an array member which is distinct from other array members on the basis of a different Bacillus licheniformis gene sequence or oligo sequence thereof, and/or different concentrations of the same or distinct Bacillus licheniformis genes and/or different mixtures of distinct Bacillus licheniformis genes or different-concentrations of Bacillus licheniformis genes. Thus an array of “distinct Bacillus licheniformis genes” may be an array containing, as its members, (i) distinct Bacillus licheniformis genes which may have a defined amount in each member, (ii) different, graded concentrations of a specific Bacillus licheniformis gene, and/or (iii) different-composition mixtures of two or more distinct Bacillus licheniformis genes.
It will be understood, however, that in the methods of the present invention, any type of substrate known in the art may be used.
The delivery of a known amount of a selected Bacillus licheniformis gene to a specific position on the support surface is preferably performed with a dispensing device equipped with one or more tips for insuring reproducible deposition and location of the Bacillus licheniformis genes and for preparing multiple arrays. Any dispensing device known in the art may be used in the methods of the present invention. See, for example, U.S. Pat. No. 5,807,522.
For liquid-dispensing on a hydrophilic surface, the liquid will have less of a tendency to bead, and the dispensed volume will be more sensitive to the total dwell time of the dispenser tip in the immediate vicinity of the support surface.
For liquid-dispensing on a hydrophobic surface, flow of fluid from the tip onto the support surface will continue from the dispenser onto the support surface until it forms a liquid bead. At a given bead size, i.e., volume, the tendency of liquid to flow onto the surface will be balanced by the hydrophobic surface interaction of the bead with the support surface, which acts to limit the total bead area on the surface, and by the surface tension of the droplet, which tends toward a given bead curvature. At this point, a given bead volume will have formed, and continued contact of the dispenser tip with the bead, as the dispenser tip is being withdrawn, will have little or no effect on bead volume.
The desired deposition volume, i.e., bead volume, formed is preferably in the range 2 pl (picoliters) to 2 nl (nanoliters), although volumes as high as 100 nl or more may be dispensed. It will be appreciated that the selected dispensed volume will depend on (i) the “footprint” of the dispenser tip(s), i.e., the size of the area spanned by the tip(s), (ii) the hydrophobicity of the support surface, and (iii) the time of contact with and rate of withdrawal of the tip(s) from the support surface. In addition, bead size may be reduced by increasing the viscosity of the medium, effectively reducing the flow time of liquid from the dispensing device onto the support surface. The drop size may be further constrained by depositing the drop in a hydrophilic region surrounded by a hydrophobic grid pattern on the support surface.
At a given tip size, bead volume can be reduced in a controlled fashion by increasing surface hydrophobicity, reducing time of contact of the tip with the surface, increasing rate of movement of the tip away from the surface, and/or increasing the viscosity of the medium. Once these parameters are fixed, a selected deposition volume in the desired picoliter to nanoliter range can be achieved in a repeatable fashion.
After depositing a liquid droplet of a Bacillus licheniformis gene sample at one selected location on a support, the tip may be moved to a corresponding position on a second support, the Bacillus licheniformis gene sample is deposited at that position, and this process is repeated until the random nucleic acid fragment sample has been deposited at a selected position on a plurality of supports.
This deposition process may then be repeated with another random nucleic acid fragment sample at another microarray position on each of the supports.
The diameter of each Bacillus licheniformis gene region is preferably between about 20-200 μm. The spacing between each region and its closest (non-diagonal) neighbor, measured from center-to-center, is preferably in the range of about 20-400 μm. Thus, for example, an array having a center-to-center spacing of about 250 μm contains about 40 regions/cm or 1,600 regions/cm2. After formation of the array, the support is treated to evaporate the liquid of the droplet forming each region, to leave a desired array of dried, relatively flat Bacillus licheniformis gene or oligo thereof regions. This drying may be done by heating or under vacuum. The DNA can also be UV-crosslinked to the polymer coating.
Nucleic Acid Probes. In the methods of the present invention, the strains are cultivated in a nutrient medium with and without a substrate using methods well known in the art for isolation of nucleic acids to be used as probes. For example, the strains may be cultivated by shake flask cultivation, small-scale or large-scale fermentation (including continuous, batch, fed-batch, or solid state fermentations) in laboratory or industrial fermentors performed in a suitable medium. The cultivation takes place in a suitable nutrient medium comprising carbon and nitrogen sources and inorganic salts, using procedures known in the art. Suitable media are available from commercial suppliers or may be prepared according to published compositions (e.g., in catalogues of the American Type Culture Collection).
The nucleic acid probes from the microbial strains cultured on medium with and without substrate may be any nucleic acid including genomic DNA, cDNA, and RNA, and may be isolated using standard methods known in the art.
The populations of isolated nucleic acid probes may be labeled with detection reporters such as colorimetric, radioactive for example, 32P, 33P, or 35S), fluorescent reporters, or other reporters using methods known in the art (Chen et al., 1998, Genomics 51: 313-324; DeRisi et al., 1997, Science 278: 680-686; U.S. Pat. No. 5,770,367).
In a preferred aspect, the probes are labeled with fluorescent reporters. For example, the DNA probes may be labeled during reverse transcription from the respective RNA pools by incorporation of fluorophores as dye-labeled nucleotides (DeRisi et al., 1997, supra), e.g., Cy5-labeled deoxyuridine triphosphate, or the isolated cDNAs may be directly labeled with different fluorescent functional groups. Fluorescent-labeled nucleotides include, but are not limited to, fluorescein conjugated nucleotide analogs (green fluorescence), lissamine nucleotide analogs (red fluorescence). Fluorescent functional groups include, but are not limited to, Cy3 (a green fluorescent dye) and Cy5 (red fluorescent dye).
Array Hybridization. The labeled nucleic acids from the two strains cultivated with and without substrate are then added to an array of Bacillus licheniformis genes under conditions where the nucleic acid pools from the two strains hybridize to complementary sequences of the Bacillus licheniformis genes on the array. For purposes of the present invention, hybridization indicates that the labeled nucleic acids from the two strains hybridize to the Bacillus licheniformis genes under very low to very high stringency conditions.
A small volume of the labeled nucleic acids mixture is loaded onto the substrate. The solution will spread to cover the entire microarray. In the case of a multi-cell substrate, one or more solutions are loaded into each cell which stop at the barrier elements.
For nucleic acid probes of at least about 100 nucleotides in length, miroarray hybridization conditions described by Eisen and Brown, 1999, Methods of Enzymology 303: 179-205, may be used. Hybridization is conducted under a cover slip at 65° C. in 3×SSC for 4-16 hours followed by post-hybridization at room temperature after removal of the cover slip in 2×SSC, 0.1% SDS by washing the array two or three times in the solution, followed by successive washes in 1×SSC for 2 minutes and 0.2×SSC wash for two or more minutes.
Conventional conditions of very low to very high stringency conditions may also be used. Very low to very high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 μg/ml sheared and denatured salmon sperm DNA, and either 25% formamide for very low and low stringencies, 35% formamide for medium and medium-high stringencies, or 50% formamide for high and very high stringencies, following standard Southern blotting procedures.
The carrier material is finally washed three times each for 15 minutes using 2×SSC, 0.2% SDS preferably at least at 45° C. (very low stringency), more preferably at least at 50° C. (low stringency), more preferably at least at 55° C. (medium stringency), more preferably at least at 60° C. (medium-high stringency), even more preferably at least at 65° C. (high stringency), and most preferably at least at 70° C. (very high stringency).
For shorter nucleic acid probes which are less than 50 nucleotides, microarray hybridization conditions described by Kane et al., 2000, Nucleic Acids Research 28: 4552-4557, may be used. Hybridization is conducted under a supported coverslip at 42° C. for 16-18 hours at high humidity in 50% formamide, 4.1× Denhardt's solution, 4.4×SSC, and 100 μg/ml of herring sperm DNA. Arrays are washed after removal of the coverslip in 4×SSC by immersion into 1×SSC, 0.1% SDS for 10 minutes, 0.1×SSC, 0.1% SDS twice for 10 minutes, and 0.1×SSC twice for 10 minutes.
For shorter nucleic acid probes which are about 50 nucleotides to about 100 nucleotides in length, conventional stringency conditions may be used. Such stringency conditions are defined as prehybridization, hybridization, and washing post-hybridization at 5° C. to 10° C. below the calculated Tm using the calculation according to Bolton and McCarthy (1962, Proceedings of the National Academy of Sciences USA 48:1390) in 0.9 M NaCl, 0.09 M Tris-HCl pH 7.6, 6 mM EDTA, 0.5% NP-40, 1× Denhardt's solution, 1 mM sodium pyrophosphate, 1 mM sodium monobasic phosphate, 0.1 mM ATP, and 0.2 mg of yeast RNA per ml following standard Southern blotting procedures.
The carrier material is finally washed once in 6×SSC plus 0.1% SDS for 15 minutes and twice each for 15 minutes using 6×SSC at 5° C. to 10° C. below the calculated Tm.
The choice of hybridization conditions will depend on the degree of homology between the Bacillus licheniformis genes and the nucleic acid probes obtained from the strain cultured with and without inducing substrate. For example, where the nucleic acid probes and the Bacillus licheniformis genes are obtained from identical strains, high stringency conditions may be most suitable. Where the strains are from a genus or species different from which the Bacillus licheniformis genes were obtained, low or medium stringency conditions may be more suitable.
In a preferred aspect, the hybridization is conducted under low stringency conditions. In a more preferred aspect, the hybridization is conducted under medium stringency conditions. In a most preferred aspect, the hybridization is conducted under high stringency conditions.
The entire solid support is then reacted with detection reagents if needed and analyzed using standard colorimetric, radioactive, or fluorescent detection means. All processing and detection steps are performed simultaneously to all of the microarrays on the solid support ensuring uniform assay conditions for all of the microarrays on the solid support.
Detection. The most common detection method is laser-induced fluorescence detection using confocal optics (Cheung et al., 1998, Nat. Genet. 18: 225-230). The array is examined under fluorescence excitation conditions such that (i) the Bacillus licheniformis genes on the array that hybridize to the first nucleic acid probes obtained from the strain cultured without inducing substrate and to the second nucleic acid probes obtained from the strain cultured with inducing substrate produce a distinct first fluorescence emission color and a distinct second fluorescence emission color, respectively, and (ii) the Bacillus licheniformis genes on the array that hybridize to substantially equal numbers of nucleic acid probes obtained from the strain cultured without inducing substrate and from the strain cultured with inducing substrate produce a distinct combined fluorescence emission color; wherein the relative expression of the genes in the strains can be determined by the observed fluorescence emission color of each spot on the array.
The fluorescence excitation conditions are based on the selection of the fluorescence reporters. For example, Cy3 and Cy5 reporters are detected with solid state lasers operating at 532 nm and 632 nm, respectively.
However, other methods of detection well known in the art may be used such as standard photometric, calorimetric, or radioactive detection means, as described earlier.
Data Analysis. The data obtained from the scanned image may then be analyzed using any of the commercially available image analysis software. The software preferably identifies array elements, subtracts backgrounds, deconvolutes multi-color images, flags or removes artifacts, verifies that controls have performed properly, and normalizes the signals (Chen et al., 1997, Journal of Biomedical Optics 2: 364-374).
Several computational methods have been described for the analysis and interpretation of microarray-based expression profiles including cluster analysis (Eisen et al., 1998, Proc. Nat. Acad. Sci. USA 95: 14863-14868), parametric ordering of genes (Spellman et al., 1998, Mol. Biol. Cell 9: 3273-3297), and supervised clustering methods based on representative hand-picked or computer-generated expression profiles (Chu et al., 1998. Science 282: 699-705). Preferred methods for evaluating the results of the microarrays employ statistical analysis to determine the significance of the differences in expression levels. In the methods of the present invention, the difference in the detected expression level is at least about 10% or greater, preferably at least about 20% or greater, more preferably at least about 50% or greater, even more preferably at least about 75% or greater; and most preferably at least about 100% or greater.
One such preferred system is the Significance Analysis of Microarrays (SAM) (Tusher et al., 2001, Proc. Natl. Acad. Sci. USA 98: 5116-5121). Statistical analysis allows the determination of significantly altered expression of levels of about 50% or even less. The PAM (or predictive analysis for microarrays) represents another approach for analyzing the results of the microarrays (Tibshirani et al., 2002, Proc. Natl. Acad. Sci. USA 99: 6567-6572).
Cluster algorithms may also be used to analyze microarray expression data. From the analysis of the expression profiles it is possible to identify co-regulated genes that perform common metabolic or biosynthetic functions. Hierarchical clustering has been employed in the analysis of microarray expression data in order to place genes into clusters based on sharing similar patterns of expression (Eisen et al., 1998, supra). This method yields a graphical display that resembles a kind of phylogenetic tree where the relatedness of the expression behavior of each gene to every other gene is depicted by branch lengths. The programs Cluster and TreeView, both written by Michael Eisen (Eisen et al., 1998 Proc. Nat. Acad. Sci. USA 95: 14863-14868) are freely available. Genespring is a commercial program available for such analysis (Silicon Genetics, Redwood City, Calif.).
Self-organizing maps (SOMs), a non-hierarchical method, have also been used to analyze microarray expression data (Tamayo et al., 1999, Proc. Natl. Acad. Sci. USA 96: 2907-2912). This method involves selecting a geometry of nodes, where the number of nodes defines the number of clusters. Then, the number of genes analyzed and the number of experimental conditions that were used to provide the expression values of these genes are subjected to an iterative process (20,000-50,000 iterations) that maps the nodes and data points into multidimensional gene expression space. After the identification of significantly regulated genes, the expression level of each gene is normalized across experiments. As a result, the expression profile of the genome is highlighted in a manner that is relatively independent of each gene's expression magnitude. Software for the “GENECLUSTER” SOM program for microarray expression analysis can be obtained from the Whitehead/MIT Center for Genome Research. SOMs can also be constructed using the GeneSpring software package.
Isolation of Genes. Probes containing genes or portions thereof identified to be induced by the present of substrate in the medium are characterized by determining the sequence of the probe. Based on the sequence, the gene can then be isolated using methods well known in the art.
The techniques used to isolate or clone a gene include isolation from genomic DNA, preparation from cDNA, or a combination thereof. The cloning of the gene from such genomic DNA can be effected, e.g., by using the well known polymerase chain reaction (PCR) or antibody screening of expression libraries to detect cloned DNA fragments with shared structural features. See, e.g., Innis et al., 1990, PCR: A Guide to Methods and Application, Academic Press, New York. Other nucleic acid amplification procedures such as ligase chain reaction (LCR), ligated activated transcription (LAT) and nucleic acid sequence-based amplification (NASBA) may be used. The gene may be cloned from the strain of interest, or another or related organism and thus, for example, may be an allelic or species variant of the gene.
Methods for Monitoring Differential Expression of a Plurality of Genes
The present invention also relates to methods for monitoring differential expression of a plurality of genes in a first bacterial cell relative to expression of the same genes in one or more second bacterial cells, comprising:
(a) adding a mixture of detection reporter-labeled nucleic acids isolated from the bacterial cells to a substrate containing an array of Bacillus licheniformis genes selected from the group consisting of nucleotides SEQ ID NOs: 2-4198, complementary strands of SEQ ID NOs: 2-4198, or fragments of SEQ ID NOs: 2-4198, under conditions where the detection reporter-labeled nucleic acids hybridize to complementary sequences of the Bacillus licheniformis genes on the array, wherein the nucleic acids from the first bacterial cell and the one or more second bacterial cells are labeled with a first detection reporter and one or more different second detection reporters, respectively; and
(b) examining the array under conditions wherein the relative expression of the genes in the bacterial cells is determined by the observed detection signal of each spot on the array in which (i) the Bacillus licheniformis genes on the array that hybridize to the nucleic acids obtained from either the first or the one or more second bacterial cells produce a distinct first detection signal or one or more second detection signals, respectively, and (ii) the Bacillus licheniformis genes on the array that hybridize to the nucleic acids obtained from both the first and one or more second bacterial produce a distinct combined detection signal.
The methods of the present invention may be used to monitor global expression of a plurality of genes from a Bacillus cell, discover new genes, identify possible functions of unknown open reading frames, and monitor gene copy number variation and stability. For example, the global view of changes in expression of genes may be used to provide a picture of the way in which Bacillus cells adapt to changes in culture conditions, environmental stress, or other physiological provocation. Other possibilities for monitoring global expression include spore morphogenesis, recombination, metabolic or catabolic pathway engineering.
The methods of the present invention are particularly advantageous since one spot on an array equals one gene or open reading frame because extensive follow-up characterization is unnecessary since sequence information is available, and the Bacillus licheniformis microarrays can be organized based on function of the gene products.
Microarrays. Methods for preparing the microarrays are described herein.
Bacterial Cells. In the methods of the present invention, the two or more Bacillus cells may be any Bacillus cell where one of the cells is used as a reference for identifying differences in expression of the same or similar complement of genes in the other cell(s). In one aspect, the two or more cells are the same cell. For example, they may be compared under different growth conditions, e.g., oxygen limitation, nutrition, and/or physiology. In another aspect, one or more cells are mutants of the reference cell. For example, the mutant(s) may have a different phenotype. In a further aspect, the two or more cells are of different species (e.g., Bacillus clausii and Bacillus subtilis). In another further aspect, the two or more cells are of different genera. In an even further aspect, one or more cells are transformants of the reference cell, wherein the one or more transformants exhibit a different property. For example, the transformants may have an improved phenotype relative to the reference cell and/or one of the other transformants. The term “phenotype” is defined herein as an observable or outward characteristic of a cell determined by its genotype and modulated by its environment. Such improved phenotypes may include, but are not limited to, improved secretion or production of a protein or compound, reduced or no secretion or production of a protein or compound, improved or reduced expression of a gene, desirable morphology, an altered growth rate under desired conditions, relief of over-expression mediated growth inhibition, or tolerance to low oxygen conditions.
The Bacillus cells may be any Bacillus cells, but preferably Bacillus alkalophilus, Bacillus amyloliquefaciens, Bacillus brevis, Bacillus cereus, Bacillus circulans, Bacillus clausii, Bacillus coagulans, Bacillus fastidiosus, Bacillus firmus, Bacillus lautus, Bacillus lentus, Bacillus licheniformis, Bacillus macerans, Bacillus megaterium, Bacillus methanolicus, Bacillus pumilus, Bacillus sphaericus, Bacillus stearothermophilus, Bacillus subtilis, or Bacillus thuringiensis cells.
In a preferred aspect, the Bacillus cells are Bacillus alkalophilus cells. In another preferred aspect, the Bacillus cells are Bacillus amyloliquefaciens cells. In another preferred aspect, the Bacillus cells are Bacillus brevis cells. In another preferred aspect, the Bacillus cells are Bacillus cereus cells In another preferred aspect, the Bacillus cells are Bacillus circulans cells. In another preferred aspect, the Bacillus cells are Bacillus clausii cells. In another preferred aspect, the Bacillus cells are Bacillus coagulans cells. In another preferred aspect, the Bacillus cells are Bacillus fastidiosus cells. In another preferred aspect, the Bacillus cells are Bacillus firmus cells. In another preferred aspect, the Bacillus cells are Bacillus lautus cells. In another preferred aspect, the Bacillus cells are Bacillus lentus cells. In another preferred aspect, the Bacillus cells are Bacillus licheniformis cells. In another preferred aspect, the Bacillus cells are Bacillus macerans cells. In another preferred aspect, the Bacillus cells are Bacillus megaterium cells. In another preferred aspect, the Bacillus cells are Bacillus methanolicus cells. In another preferred aspect, the Bacillus cells are Bacillus pumilus cells. In another preferred aspect, the Bacillus cells are Bacillus sphaericus cells. In another preferred aspect, the Bacillus cells are Bacillus stearothermophilus cells. In another preferred aspect, the Bacillus cells are Bacillus subtilis cells. In another preferred aspect, the Bacillus cells are Bacillus thuringiensis cells.
In a more preferred aspect, the Bacillus cells are Bacillus licheniformis cells. In a most preferred aspect, the Bacillus licheniformis cells are Bacillus licheniformis ATCC 14580 cells.
In another more preferred aspect, the Bacillus cells are Bacillus clausii cells. In another most preferred aspect, the Bacillus clausii cells are Bacillus clausii NCIB 10309 cells.
It will be understood that the term “Bacillus” also encompasses relatives of Bacillus such as Paenibacillus, Oceanobacillus, and the like. 
In the methods of the present invention, the cells are cultivated in a nutrient medium suitable for growth using methods well known in the art for isolation of the nucleic acids to be used as probes. For example, the cells may be cultivated by shake flask cultivation, small-scale or large-scale fermentation (including continuous, batch, fed-batch, or solid state fermentations) in laboratory or industrial fermentors performed in a suitable medium. The cultivation takes place in a suitable nutrient medium comprising carbon and nitrogen sources and inorganic salts, using procedures known in the art. Suitable media are available from commercial suppliers or may be prepared according to published compositions (e.g., in catalogues of the American Type Culture Collection).
Nucleic Acid Probes. The nucleic acid probes from the two or more Bacillus cells may be any nucleic acid including genomic DNA, cDNA, and RNA, and may be isolated using standard methods known in the art, as described herein. The populations of isolated nucleic acid probes may be labeled with calorimetric, radioactive, fluorescent reporters, or other reporters using methods described herein.
In a preferred aspect, the probes are labeled with fluorescent reporters, e.g., Cy3 (a green fluorescent dye) and Cy5 (red fluorescent dye), as described herein.
Array Hybridization. The labeled nucleic acids from the two or more Bacillus cells are then added to a substrate containing an array of Bacillus licheniformis genes under conditions, as described herein, where the nucleic acid pools from the two or more Bacillus cells hybridize to complementary sequences of the Bacillus licheniformis genes on the array.
Detection and Data Analysis. The same methods as described herein are used for detection and data analysis.
Computer Readable Media and Computer-Based Systems
The Bacillus licheniformis chromosome and its genes described herein may be “provided” in a variety of media to facilitate their use. The term “provided” refers to a manufacture comprising an array of Bacillus licheniformis genes. Such manufactures provide the Bacillus licheniformis genes in a form which allows one skilled in the art to examine the manufacture using means not directly applicable to examining the chromosome or a subset thereof as it exists in nature or in purified form.
Thus, the present invention also relates to such a manufacture in the form of a computer readable medium comprising an array of Bacillus licheniformis genes selected from the group consisting of nucleotides SEQ ID NOs: 2-4198, complementary strands of SEQ ID NOs: 2-4198, or fragments of SEQ ID NOs: 2-4198.
In one application of this aspect, the Bacillus licheniformis genes of the present invention can be recorded on computer readable media. The term “computer readable media” is defined herein as any medium which can be read and accessed by a computer. Such computer readable media include, but are not limited to, magnetic storage media, e.g., floppy discs, hard disc storage medium, and magnetic tape; optical storage media, e.g., CD-ROM, DVD; electrical storage media, e.g., RAM and ROM; and hybrids of these categories, e.g., magnetic/optical storage media. One skilled in the art can readily appreciate how any of the presently known computer readable media can be used to create a manufacture comprising computer readable medium having recorded thereon a nucleotide sequence of the present invention. Likewise, it will be clear to those of skill how additional computer readable media that may be developed also can be used to create analogous manufactures having recorded thereon a nucleotide sequence of the present invention.
As used herein, “recorded” refers to a process for storing information on computer readable medium. One skilled in the art can readily adopt any of the presently known methods for recording information on computer readable medium to generate manufactures comprising the nucleotide sequence information of the present invention.
A variety of data storage structures are available for creating a computer readable medium having recorded thereon a nucleotide sequence of the present invention. The choice of the data storage structure will generally be based on the means chosen to access the stored information. In addition, a variety of data processor programs and formats can be used to store the nucleotide sequence information of the present invention on computer readable medium. The sequence information can be represented in a word processing text file, formatted in commercially-available software such as WordPerfect and Microsoft Word, or represented in the form of an ASCII file, stored in a database application, such as DB2, Sybase, Oracle, or the like. A skilled artisan can readily adapt any number of data-processor structuring formats (e.g., text file or database) in order to obtain computer readable medium having recorded thereon the nucleotide sequence information of the present invention.
Various computer software programs are publicly available that allow a skilled artisan to access sequence information provided in a computer readable medium. Thus, by providing in computer readable form an array of Bacillus licheniformis genes selected from the group consisting of nucleotides SEQ ID NOs: 2-4198, complementary strands of SEQ ID NOs: 2-4198, or fragments of SEQ ID NOs: 2-4198, enables one skilled in the art to routinely access the provided sequence information for a wide variety of purposes.
Software utilizing the BLAST (Altschul et al., 1990, supra), BLAZE (Brutlag et al., 1993, Comp. Chem. 17: 203-207), GENEMARK (Lukashin and Borodovsky, 1998, Nucleic Acids Research 26: 1107-1115), GENSCAN (Burge and Karlin, 1997, Journal of Molecular Biology 268: 78-94), GLIMMER (Salzberg et al., 1998, Nucleic Acids Research 26: 544-548), and GRAIL (Xu et al., 1994, Comput. Appl. Biosci. 10: 613-623) search algorithms may be used to identify open reading frames (ORFs) within a genome of interest, which contain homology to ORFs or proteins from both Bacillus licheniformis and Bacillus clausii and from other organisms. Among the ORFs discussed herein are protein encoding fragments of the Bacillus licheniformis and Bacillus clausii genomes useful in producing commercially important proteins, such as enzymes used in fermentation reactions and in the production of commercially useful metabolites.
The present invention further provides systems, particularly computer-based systems, which contain the sequence information described herein. Such systems are designed to identify, among other things, genes and gene products—many of which could be products themselves or used to genetically modify an industrial expression host through increased or decreased expression of a specific gene sequence(s).
The term “a computer-based system” is herein defined as the hardware means, software means, and data storage means used to analyze the nucleotide sequence information of the present invention. The minimum hardware means of the computer-based systems of the present invention comprises a central processing unit (CPU), input means, output means, and data storage means. One skilled in the art can readily appreciate that any currently available computer-based system is suitable for use in the present invention.
As stated above, the computer-based systems of the present invention comprise a data storage means having stored therein a nucleotide sequence of the present invention and the necessary hardware means and software means for supporting and implementing a search means.
The term “data storage means” is defined herein as memory which can store nucleotide sequence information of the present invention, or a memory access means which can access manufactures having recorded thereon the nucleotide sequence information of the present invention.
The term “search means” refers is defined herein as one or more programs which are implemented on the computer-based system to compare a target sequence or target structural motif with the sequence information stored within the data storage means. Search means are used to identify fragments or regions of the present genomic sequences which match a particular target sequence or target motif. A variety of known algorithms are disclosed publicly and a variety of commercially available software for conducting search means are and can be used in the computer-based systems of the present invention. Examples of such software includes, but is not limited to, MacPattern (Fuchs, 1991, Comput. Appl. Biosci. 7: 105-106), BLASTN and BLASTX National Center for Biotechnology Information (NCBI). One skilled in the art can readily recognize that any one of the available algorithms or implementing software packages for conducting homology searches can be adapted for use in the present computer-based systems.
The term “target sequence” is defined here as any DNA (genomic DNA, cDNA) or amino acid sequence of six or more nucleotides or two or more amino acids. One skilled in the art can readily recognize that the longer a target sequence is, the less likely a target sequence will be present as a random occurrence in the database. The most preferred sequence length of a target sequence is from about 10 to 100 amino acids or from about 30 to 300 nucleotide residues. However, it is well recognized that searches for commercially important fragments, such as sequence fragments involved in gene expression and protein processing, may be of shorter length.
The term “a target structural motif” or “target motif” is defined herein as any rationally selected sequence or combination of sequences in which the sequence(s) are chosen based on a three-dimensional configuration which is formed upon the folding of the target motif. There are a variety of target motifs known in the art. Protein target motifs include, but are not limited to, enzyme active sites and signal sequences, substrate and cofactor binding domains, transmembrane domains, and sites for post-translational modifications. Nucleic acid target motifs include, but are not limited to, promoter sequences, hairpin structures and inducible expression elements (protein binding sequences), repeats, palindromes, dyad symmetries, and transcription and translation start and stop sites.
A variety of structural formats for the input and output means can be used to input and output the information in the computer-based systems of the present invention. A preferred format for an output means ranks fragments of the Bacillus licheniformis or Bacillus clausii genomic sequences possessing varying degrees of homology to the target sequence or target motif. Such presentation provides one skilled in the art with a ranking of sequences which contain various amounts of the target sequence or target motif and identifies the degree of homology contained in the identified fragment.
A variety of comparing means can be used to compare a target sequence or target motif with the data storage means to identify sequence fragments of the Bacillus licheniformis and Bacillus clausii genomes. For example, implementing software which utilize the BLAST and BLAZE algorithms, described in Altschul et al., 1990, supra, may be used to identify open reading frames within the Bacillus licheniformis or Bacillus clausii genome or the genomes of other organisms. A skilled artisan can readily recognize that any one of the publicly available homology search programs can be used as the search means for the computer-based systems of the present invention. Suitable proprietary systems that may be known to those of skill also may be employed in this regard.
Codon Usage Tables
The present invention further relates to methods for preparing a synthetic gene, comprising (a) generating a codon usage table based on codons used in one or more open reading frames or portions thereof of SEQ ID NO: 1, (b) constructing a synthetic gene or portion thereof that contains in place of one or more native codons one or more preferred codons from the codon usage table, and (c) recovering the synthetic gene. In a preferred aspect, the codon usage table is Table 4 and/or Table 5.
The Bacillus licheniformis chromosomal sequence of SEQ ID NO: 1 or portions thereof can be used to generate codon usage tables to design synthetic genes for their efficient heterologous expression in Bacillus licheniformis host cells. The codon usage tables can be based on (1) the codon used in all the open reading frames, (2) selected open reading frames, (3) fragments of the open reading frames, or (4) fragments of selected open reading frames. With a codon usage table, synthetic genes can be designed with only the most preferred codon for each amino acid; with a number of common codons for each amino acid; or with the same or a similar statistical average of codon usages found in the table of choice.
The synthetic gene can be constructed using any method such as site-directed mutagenesis or PCR generated mutagenesis in accordance with methods known in the art. Although, in principle, the modification may be performed in vivo, i.e., directly on the cell expressing the nucleotide sequence to be modified, it is preferred that the modification is performed in vitro.
The synthetic gene can be further modified by operably linking the synthetic gene to one or more control sequences which direct the expression of the coding sequence in a suitable host cell under conditions compatible with the control sequences using the methods described herein. Nucleic acid constructs, recombinant expression vectors, and recombinant host cells comprising the synthetic gene can also be prepared using the methods described herein.
The present invention also relates to methods for producing a polypeptide encoded by such a synthetic gene comprising (a) cultivating a host cell comprising the synthetic gene under conditions conducive for production of the polypeptide; and (b) recovering the polypeptide.
The present invention is further described by the following examples which should not be construed as limiting the scope of the invention.
EXAMPLES
Example 1
Shotgun DNA Sequencing and Genome Assembly
The genome of the type strain Bacillus licheniformis ATCC 14580 was sequenced by a combination of the whole genome shotgun method described by Wilson, R. K. and Mardis, E. R., 1997, In Genome Analysis: A Laboratory Manual, Vol. 1, eds. Birren, B., Green, E. D., Meyers, R. M., and Roskams, J. (Cold Spring Harbor Press, Cold Spring Harbor, N.Y.), pp. 397-454, and fosmid end sequencing (Kim, U. J., Shizuya, H., de Jong, P. J., Birren, B. and Simon, M. I., 1992, Nucleic Acids Res. 20: 1083-1085; Longmire, J. L. and Brown, N.C., 2003, Biotechniques 35: 50-54; Zhao, S., Malek, J., Mahairas, G., Fu, L., Nierman, W., Venter, J. C., and Adams, M. D., 2000, Genomics 63: 321-332).
Genomic DNA of Bacillus licheniformis ATCC 14580 was isolated using the following method: A single colony was used to inoculate 20 ml of LB broth (Davis, R. W., Botstein, D., and Roth, J. R. 1980, Advanced Bacterial Genetics, Cold Spring Harbor Press, Cold Spring Harbor, N.Y.) in a sterile 125 ml Erlenmeyer flask. The culture was incubated at 37° C. overnight with agitation at 240 rpm. The resulting cells were collected by centrifugation in a 45 ml Oak Ridge tube for 10 minutes at 6000×g, and the cell pellet was resuspended in 5 ml of Tris-glucose buffer (50 mM Tris-HCl, pH 8.0, 50 mM glucose, 10 mM EDTA). Lysozyme was added to a final concentration of 50 μg/ml and the suspension was incubated in a 37° C. water bath for 25 minutes. Next, 200 μl of 10% SDS was added and the tubes were gently inverted several times. Five milliliters of a second detergent mixture (1% Brij, 1% deoxycholate, 50 mM EDTA, pH 7.5) was added, and the tubes were inverted several times while incubating for 20 minutes at room temperature. An equal volume of phenol:chloroform (1:1 v/v) was added and the tubes were inverted gently at room temperature for 20-30 minutes. The tubes were centrifuged for 20 minutes at 12,000×g, 4° C. The top aqueous layer was carefully removed with a wide-bore pipette and placed in a clean 45 ml Oak Ridge tube. The phenol;chloroform extraction was repeated and 1/10 volume of 3 M sodium acetate pH 5.2 was added to the aqueous layer. Two volumes of cold ethanol were carefully layered on top and the DNA was spooled from the solution onto a sterile glass rod. Spooled DNA was carefully rinsed in 70% ethanol and resuspended in a suitable amount of TE buffer (10 mM Tris-HCl, pH 8.0, 1 mM EDTA).
Plasmid libraries were constructed using randomly-sheared and BamHI-digested genomic DNA that was enriched for 2-3 kb fragments by preparative agarose gel electrophoresis (Berka, R. M., Schneider, P., Golightly, E. J., Brown, S. H., Madden, M., Brown, K. M., Halkier, T., Mondorf, K., and Xu, F., 1997, Appl. Environ. Microbiol. 63: 3151-3157). Approximately 49,000 random clones were sequenced using dye-terminator chemistry (Applied Biosystems, Foster City, Calif.) with ABI 377 and ABI 3700 automated sequencers yielding approximately 6× coverage of the genome. A combination of methods was employed for gap closure including sequencing on fosmids (Kim, U. J., Shizuya, H., de Jong, P. J., Birren, B., and Simon, M. I., 1992, Nucleic Acids Res. 20: 1083-1085), primer walking on selected clones, and PCR-amplified DNA fragments. Fosmid libraries were constructed using a commercial kit from Epicentre (Madison. WI). Data from both ends of approximately 1975 fosmid clones with an average insert size of 40 kb were incorporated to aid in validation of the final assembly. In total, the number of input reads was 62,685 with 78.6% of these incorporated into the final assembly. Sequences were base called using TraceTuner 2.0 (Paracel, Inc., Pasadena, Calif.) and assembled using the Paracel Genome Assembler (Paracel, Inc., Pasadena, Calif.) with optimized parameters and the quality score set to >20. Phrap, Crossmatch, and Consed were used for sequence finishing (Gordon D., Abajian C., and Green P., 1998, Genome Res. 8: 195-202).
Example 2
Identification And Annotation of Open Reading Frames (ORFs)
Protein coding regions in the assembled genome sequence data were identified using EasyGene (Larsen, T. S., and Krogh, A., 2003, BMC Bioinformatics 4: 21), Glimmer (Delcher, A, L., Harmon, D., Kasif, S., White, O. and Salzberg, S. L., 1999, Nucleic Acids Res. 27, 4636-4641), and FrameD (Schiex, T., Gouzy, J., Moisan, A. and de Oliveira, Y., 2003, Nucleic Acids Res. 31, 3738-3741). Only EasyGene gene models with an R-value of less than 2 and log-odds score greater than −10 were used. Predicted proteins were compared to the non-redundant database PIR-NREF (Wu, C. H., Huang, H., Arminski, L., Castro-Alvear, J., Chen, Y., Hu, Z. Z., Ledley, R. S., Lewis, K. C., Mewes, H. W., Orcutt, B. C., 2002, Nucleic Acids Research 30: 35-37) and the Bacillus subtilis genome (SubtilList) using BLASTP with an E-value threshold of 10−5. InterProScan was used to predict putative function. (Zdobnov, E. M. and Apweiler, R., 2001, Bioinformatics 17, 847-848). The InterPro analysis included comparison to Pfam (Bateman, A., Coin, L., Durbin, R., Finn, R. D., Hollich, V., Griffiths-Jones, S., Khanna, A., Sonnhammer, E. L. et al., 2004, Nucleic Acids Res. 32, D138-D141), TIGRfam (Haft, D. J., Selengut, J. D. and White, O., 2003, Nucleic Acids Res. 31: 371-373), Interpro (Apweiler, R., Attwood, T. K., Bairock, A., Bateman, A., Birney, E., Biswas, M., Bucher, P., Cerutti, L., Corpet, F., Croning, M. D., et al., 2001, Nucleic Acids Res. 29: 37-40), signal peptide prediction using SignalP (Nielsen, H., Engelbrecht, J., Brunak, S., and von Heijne, G., 1997, Protein Engineering 10: 1-6), and trans-membrane domain prediction using TMHMM (Krogh, A., Larsson, B., von Heijne, G. and Sonnhammer, E. L. L., 2000, J. Mol. Biol. 305, 567-580).
These ORFs were assigned to functional categories based on the Cluster of Orthologous Groups (COG) database with manual verification as described (Tatusov, R. L., Koonin, E. V. and Lipman, D. J., 1997, Science 278: 631-637; Koonin, E. V. and Galperin, M. Y., 2002, Sequence-Evolution-Function: Computational Approaches in Comparative Genomics (Kluwer, Boston)). Transfer RNA genes were identified using tRNAscan-SE (Lowe, T. M. and Eddy, S. R., 1997, Nucleic Acids Res. 25: 955-964).
Example 3
General Features of the Bacillus licheniformis Genome
The genome of Bacillus licheniformis ATCC 14580 was determined to consist of a circular molecule of 4,222,336 bp with an average GC content of 46.2% (Table 2). No plasmids were found during the genome analysis, and none were found by agarose gel electrophoresis.
The genome contains 4208 predicted protein-coding genes with an average size of 873 bp, seven rRNA operons, and 81 tRNA genes. Using a combination of several gene-finding algorithms 4208 protein coding ORFs were predicted. These ORFs constitute 87% of the genome and have an average length of 873 bp. Approximately 48% of the ORFs are encoded on one DNA strand and 52% on the other strand. Among the protein coding ORFs, 3948 (94%) have significant similarity to proteins in PIR, and 3187 of these gene models contain Interpro motifs and 2895 contain protein motifs found in PFAM. The number of hypothetical and conserved hypothetical proteins in the Bacillus licheniformis genome with hits in the PIR database was 1318 (212 conserved hypothetical ORFs). Among the list of hypothetical and conserved hypothetical ORFs, 683 (52%) have protein motifs contained in PFAM (148 conserved hypothetical ORFs). There are 72 tRNA genes representing all 20 amino acids and 7 rRNA operons.
The likely origin of replication was identified by similarities to several features of Bacillus subtilis origin (Moriya, S., Fukuoka, T., Ogasawara, N., and Yoshikawa, H., 1988, EMBO Journal 7: 2911-2917; Ogasawara, N., Nakai, S., and Yoshikawa, H., 1994, DNA Res. 1, 1-14; Kadoya, R., Hassan, A. K., Kasahara, Y., Ogasawara, N., and Moriya, S., 2002, Mol. Microbiol. 45: 73-87; Tosato, V., Gjuracic, K., Vlahovicek, K., Pongor, S., Danchin, A., and Bruschi, C. V., 2003, FEMS Microbiol. Lett 218: 23-30). These included (a) colocalization of four genes (rpmH, dnaA, dnaN, and recF) found near the origin of the Bacillus subtilis chromosome, (b) GC nucleotide skew [(G−C)/(G+C)] analysis, and (c) the presence of multiple dnaA-boxes (Pedersen, A. G., Jensen, L. J., Brunak, S., Staerfeldt, H. H., and Ussery, D. W., 2000, Mol. Biol. 299: 907-930; Christensen, B. B., Atlung, T., and Hansen, F. G., 1999, J. Bacteriol. 181: 2683-2688; Majka, J., Jakimowicz, D., Messer, W., Schrempf, H., Lisowski, M., and Zakrzewska-Czerwińska, J., 1999, Eur. J. Biochem. 260: 325-335) and AT-rich sequences in the region immediately upstream of the dnaA gene. On the basis of these observations, a cytosine residue of the BstBl restriction site was assigned between the rpmH and dnaA genes to be the first nucleotide of the Bacillus licheniformis genome. The replication termination site was localized near 2.02 Mb by GC skew analysis. This region lies roughly opposite the origin of replication.
Unlike Bacillus subtilis, no apparent gene encoding a replication terminator protein (rtp) was found in Bacillus licheniformis. The Bacillus halodurans genome also lacks an rtp function (Takami, H., Nakasone, K., Takaki, Y., Maeno, G., Sasaki, R., Masui, N., Fuji, F., Hirama, C., Nakamura, Y., Ogasawara, N. et al., 2000, Nucleic Acids Res. 28: 4317-4331), and it seems likely that Bacillus subtilis acquired the rtp gene following its divergence from Bacillus halodurans and Bacillus licheniformis. 
Transposable elements and prophages. The genome of Bacillus licheniformis ATCC 14580 was determined to contain nine identical copies of a 1285 bp insertion sequence element termed IS3BIi1 (Lapidus, A., Galleron, N., Andersen, J. T., Jørgensen, P. L. Ehrlich, S. D., and Sorokin, A., 2002, FEMS Microbiol. Lett. 209: 23-30). This sequence shares a number of features with other IS3 family elements including direct repeats of three to five bp, a ten bp left inverted repeat, and a nine bp right inverted repeat. IS3BIi1 encodes two predicted overlapping ORFs, designated orfA and orfB in relative translational reading frames of 0 and −1. The presence of a “slippery heptamer” motif, AAAAAAG, before the stop codon in orfA suggests that programmed translational frameshifting occurs between these two ORFs, resulting in a single gene product (Farabaugh, P., 1996, Microbiol. Rev. 60: 103-134). The orfB gene product harbors the DD[35]E[7]K motif, a highly conserved pattern among insertion sequences. Eight of the IS3BIi1 elements lie in intergenic regions, and one interrupts the comP gene. In addition to these insertion sequences, the genome encodes a putative transposase that is most closely related (E=1.8e−11) to one identified in the Thermoanaerobacter tengcongensis genome (Bao, Q., Tian, Y., Li, W., Xu, Z.; Xuan, Z., Hu, S., Dong, W., Yang, J., Chen, Y., Xue, Y., et al., 2002, Genome Res. 12: 689-700), however, similar genes are also found in the chromosomes of Bacillus halodurans (Takami, H., Nakasone, K., Takaki, Y., Maeno, G., Sasaki, R., Masui, N., Fuji, F., Hirama, C., Nakamura, Y., Ogasawara, N. et al., 2000, Nucleic Acids Res. 28: 4317-4331), Oceanobacillus iheyensis (Takami, H., Takaki, Y., and Uchiyama, I., 2002, Nucleic Acids Res. 30: 3927-3935.), Streptococcus agalactiae (Takahashi, S., Detrick, S., Whiting, A. A, Blaschke-Bonkowksy, A. J., Aoyagi, Y., Adderson, E. E., and Bohnsack, J. F., 2002, J. Infect Dis. 186: 1034-1038), and Streptococcus pyogenes (Smoot, J. C., Barbian, K. D., Van Gompel, J. J., Smoot, L. M., Chaussee, M. S., Sylva, G. L., Sturdevant, D. E., Ricklefs, S. M., Porcella, S. F., Parkins, L. D., et al., 2002, Proc. Natl. Acad. Sci. U.S.A. 99: 4668-4673).
The presence of several bacteriophage lysogens or prophage-like elements was revealed by Smith-Waterman comparisons to other bacterial genomes and by their AT-rich signatures. Prophage sequences, designated NZP1 and NZP3 (similar to PBSX and φ-105, respectively), were uncovered by noting the presence of nearby genes encoding the large subunit of terminase, a signature protein that is highly conserved among prophages (Casjens, S., 2003, Mol. Microbiol. 49: 277-300). A terminase gene was not observed in a third putative prophage, termed NZP2 (similarity to SPP1), however, its absence may be the result of genome deterioration during evolution. Regions were observed in which the GC content is less than 39% usually encoded proteins that have no Bacillus subtilis orthologue and share identity only to hypothetical and conserved hypothetical genes. Two of these AT-rich segments correspond to the NZP2 and NZP3 prophages.
An isochore plot also revealed the presence of a region with an atypically high (62%) G+C content. This segment contains two hypothetical ORFs whose sizes (3831 and 2865 bp) greatly exceed the size of an average gene in Bacillus licheniformis. The first protein encodes a protein of 1277 amino acids for which Interpro predicted 16 collagen triple helix repeats, and the amino acid pattern TGATGPT is repeated 75 times within the polypeptide. The second ORF is smaller, and encodes a protein with 11 collagen triple helix repeats, and the same TGATGPT motif recurs 56 times. Interestingly, the chromosomal region (19 kb) adjacent to these genes is clearly non-colinear with the Bacillus subtilis genome, and virtually all of the predicted ORFs are hypothetical or conserved hypothetical proteins. There are a number of bacterial proteins listed in PIR that contain collagen triple helix repeat regions including two from Mesorhizobium loti (accession numbers NF00607049 and NF00607035) and three from Bacillus cereus (accession numbers NF01692528, NF01269899, and NF01694666). These putative orthologs share 53-76% amino acid sequence identity with their counterparts in Bacillus licheniformis, although their functions are unknown.
Extracellular enzymes. In the Bacillus licheniformis genome, 689 of the 4208 gene models have signal peptides as forecasted by SignalP (Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Henrik Nielsen, Jacob Engelbrecht, Søren Brunak and Gunnar von Heijne, 1997, Protein Engineering 10: 1-6). Of these, 309 have no trans-membrane domain as predicted with TMHMM (A. Krogh, B. Larsson, G. von Heijne, and E. L. L. Sonnhammer, 2000, Journal of Molecular Biology 305: 567-580) and 134 are hypothetical or conserved hypothetical genes. Based on a manual examination of the remaining 175 ORFs, at least 82 were determined to likely encode secreted proteins and enzymes. The sequence ID numbers for each of these genes are listed in Table 3.
Protein secretion, sporulation, and competence pathways. Kunst et al. (Kunst, F., Ogasawara, N, Mozser, I., Albertini, A. M., Alloni, G., Azebedo, V., Bertero, M. G., Bessieres, P., Bolotin, A., and Borchert, S., 1997, Nature 390: 249-256) listed 18 genes that play a major role in the secretion of extracellular enzymes by Bacillus subtilis 168. This list includes several chaperonins, signal peptidases, components of the signal recognition particle and protein translocase complexes. Thus, it is reasonable to expect that the central features of the secretory apparatus are conserved in Bacillus subtilis and Bacillus licheniformis. 
From the list of 139 sporulation genes tabulated by Kunst et al. (Kunst, F., Ogasawara, N, Mozser, I., Albertini, A. M., Alloni, G., Azebedo, V., Bertero, M. G., Bessieres, P., Bolotin, A., and Borchert, S. et al., 1997, Nature 390: 249-256), all but six have obvious counterparts in Bacillus licheniformis. These six exceptions (spsABCEFG) comprise an operon involved in synthesis of spore coat polysaccharide in Bacillus subtilis. Additionally, the response regulator gene family (phrACEFGI) appears to have a low level of sequence conservation between Bacillus subtilis and Bacillus licheniformis. 
Natural competence (the ability to take up and process exogenous DNA in specific growth conditions) is a feature of few Bacillus licheniformis strains (Gwinn, D. D. and Thorne, C. B., 1964, J. Bacteriol. 87: 519-526). The reasons for variability in competence phenotype have not been explored at the genetic level, but the genome data offered several possible explanations. Although the type strain genome encodes all of the late competence functions ascribed in Bacillus subtilis (e.g., comC, comEFG operons, comk, mecA), it lacks an obvious comS gene, and the comP gene is punctuated by an insertion sequence element, suggesting that the early stages of competence development have been preempted in Bacillus licheniformis ATCC 14580. Whether these early functions can be restored by introducing the corresponding genes from Bacillus subtilis is unknown. In addition to an apparent deficiency in DNA uptake, two Type I restriction-modification systems were discovered that may also contribute diminished transformation efficiencies. These are distinct from the ydiOPS genes of Bacillus subtilis, and could participate in degradation of improperly modified DNA from heterologous hosts used during construction of recombinant expression vectors. Lastly, the synthesis of a glutamyl polypeptide capsule has also been implicated as a potential barrier to transformation of Bacillus licheniformis strains (Thorne, C. B. and Stull, H. B., 1966, J. Bacteriol. 91: 1012-1020). Six genes were predicted (ywtABDEF and ywsC orthologues) that may be involved in the synthesis of this capsular material.
Antibiotics and secondary metabolites. Bacitracin is a cyclic peptide antibiotic that is synthesized non-ribosomally in Bacillus licheniformis (Katz, E. and Demain, A. L., 1977, Bacteriol. Rev. 41: 449-474). White there is variation in the prevalence of bacitracin synthase genes in laboratory strains of this species, one study suggested that up to 50% may harbor the bac operon (Ishihara, H., Takoh, M., Nishibayashi, R., and Sato, A., 2002, Curr. Microbiol. 45: 18-23). The bac operon was determined not to be present in the type strain (ATCC 14580) genome. Seemingly, the only non-ribosomal peptide synthase operon encoded by the Bacillus licheniformis type strain genome is that which is responsible for lichenysin biosynthesis. Lichenysin structurally resembles surfactin from Bacillus subtilis (Grangemard, I., Wallach, J., Maget-Dana, R., and Peypoux, F., 2001, Appl. Biochem. Biotechnol. 90: 199-210), and their respective biosynthetic operons are highly similar. No Bacillus licheniformis counterparts were found for the pps (plipastatin synthase) and polyketide synthase (pks) operons of Bacillus subtilis. Collectively, these two regions represent sizeable portions (80 kb and 38 kb, respectively) of the chromosome in Bacillus subtilis, although they are reportedly dispensable (Westers, H., Dorenbos, R., van Dijl, J. M., Kable, J., Flanagan, T., Devine, K. M., Jude, F., Séror, S. J., Beekman, A. C., Darmon, E., 2003, Mol. Biol. Evol. 20: 2076-2090). Unexpectedly, a gene cluster was found encoding a lantibiotic and associated processing and transport functions. This peptide of 69 amino acids was designated as lichenicidin, and its closest known orthologue is mersacidin from Bacillus sp. strain HIL-Y85/54728 (Altena, K., Guder, A., Cramer, C., and Bierbaum, G., 2000, Appl. Environ. Microbiol. 66: 2565-2571). Lantibiotics are ribosomally synthesized peptides that are modified post-translationally so that the final molecules contain rare thioether amino acids such as lanthionine and/or methyl-lanthionine (Pag, U. and Sahl, H. G., 2002, Curr. Pharm. Des. 8: 815-833). These antimicrobial compounds have attracted much attention in recent years as models for the design of new antibiotics (Hoffmann, A., Pag, U., Wiedemann, I., and Sahl, H. G., 2002, Farmaco. 57: 685-691).
Essential Genes. The gene models were also compared to the list of essential genes in Bacillus subtilis (Kobayashi, K., Ehrlich, S. D., Albertini, A., Amati, G., Andersen, K. K., Arnaud, M., Asai, K., Ashikaga, S., Aymerch, S., Bessieres, P., 2003, Proc. Natl Acad. Sci. USA 100: 4678-4683). All the essential genes in Bacillus subtilis have orthologues in Bacillus licheniformis, and most are present in a wide range of bacterial taxa (Pedersen, P. B., Bjørnvad, M. E., Rasmussen, M. D., and Petersen, J. N., 2002, Reg. Toxicol. Pharmacol. 36: 155-161).
Example 4
Comparison of Bacillus licheniformis Genome with Other Bacilli
VisualGenome software (Rational Genomics, San Francisco, Calif.) was used for GC-skew analysis and global homology comparisons of the Bacillus licheniformis, Bacillus subtilis, and Bacillus halodurans genomes with pre-computed BLAST results stored in a local database. In pairwise comparisons (E-score threshold of 10−5) 66% (2771/4208) of the predicted Bacillus licheniformis ORFs have orthologs in Bacillus subtilis, and 55% (2321/4208) of the gene models are represented by orthologous sequences in Bacillus halodurans. Using a reciprocal BLASTP analysis 1719 orthologs were found that are common to all three species (E-score threshold of 10−5).
As noted by Lapidus et al., (Lapidus, A., Galleron, N., Andersen, J. T., Jørgensen, P. L. Ehrlich, S. D., and Sorokin, A., 2002, FEMS Microbiol. Lett. 209: 23-30), there are broad regions of colinearity between the genomes of Bacillus licheniformis and Bacillus subtilis. Less conservation of genome organization exists between Bacillus licheniformis and Bacillus halodurans, and inversion of one or more large genomic segments is evident. Clearly this supports previous findings (Xu, D. and Côté, J. C., 2003, Internat. J. Syst. Evol. Microbiol. 53: 695-704) that Bacillus subtilis and Bacillus licheniformis are phylogenetically and evolutionarily closer than either species is to Bacillus halodurans. However, a number of important differences were also observed, both in the numbers and locations of prophages and transposable elements and in a number of biochemical pathways, which distinguish Bacillus licheniformis from Bacillus subtilis, including a region of more than 80 kb that comprises a cluster of polyketide synthase genes that are absent in Bacillus licheniformis. 
Example 5
Codon Usage Tables
The evolution of codon bias, the unequal usage of synonomous codons, is thought to be due to natural selection for the use of preferred codons that match the most abundant species of isoaccepting tRNAs, resulting in increased translational efficiency and accuracy. The practical applications for utilizing codon bias information include optimizing expression of heterologous and mutant genes (Jiang and Mannervik, 1999, Protein Expression and Purification 15: 92-98), site-directed mutagenesis to derive variant polypeptides from a given gene (Wong et al., 1995, J. Immunol. 154: 3351-3358; Kaji, H. et al., 1999, J. Biochem. 126: 769-775), design and synthesis of synthetic genes (Libertini and Di Donato, 1992, Protein Engineering 5: 821-825; Feng et al., 2000, Biochem. 39: 15399-15409), and fine-tuning or reducing of translation efficiency of specific genes by introduction of non-preferred codons (Crombie, T. et al., 1992, J. Mol. Biol. 228: 7-12; Carlini and Stephan, 2003, Genetics 163: 239-243).
A codon usage table (Table 4) was generated from SEQ ID NO: 1 with CUSP, a software component of the EMBOSS package (Rice, Longden, and Bleasby, 2000, EMBOSS: The European Molecular Biology Open Software Suite. Trends in Genetics 16: 276-277) on all the predicted protein-coding genes of the Bacillus licheniformis chromosome. CUSP read the coding sequences and calculated the codon frequency table shown in Table 4.
A codon usage table (Table 5) was also generated based on the signal peptides of the 82 extracellular proteins described in Example 3.







TABLE 1







Predicted functions















Bacillus






subtilis






homolog


SEQ ID



(Gene


NO.
Description
UniRef Accession No.
Organism
Name)














2
Chromosomal replication initiator
UniRef100_P05648
Bacillus subtilis
DnaA



protein dnaA [Bacillus subtilis]


3
DNA polymerase III, beta chain
UniRef100_P05649
Bacillus subtilis
DnaN



[Bacillus subtilis]


4


5
DNA replication and repair protein recF
UniRef100_P05651
Bacillus subtilis
RecF



[Bacillus subtilis]


6


7
DNA gyrase subunit B [Bacillus subtilis]
UniRef100_P05652
Bacillus subtilis
GyrB


8
DNA gyrase subunit A [Bacillus subtilis]
UniRef100_P05653
Bacillus subtilis
GyrA


9



YaaC


10
Inosine-5′-monophosphate
UniRef100_P21879
Bacillus subtilis
GuaB



dehydrogenase [Bacillus subtilis]


11
D-alanyl-D-alanine carboxypeptidase
UniRef100_P08750
Bacillus subtilis
DacA



precursor [Bacillus subtilis]


12
Pyridoxine biosynthesis protein pdx1
UniRef100_P37527
Bacillus subtilis
YaaD



[Bacillus subtilis]


13
Hypothetical UPF0030 protein yaaE
UniRef100_P37528
Bacillus subtilis
YaaE



[Bacillus subtilis]


14
Seryl-tRNA synthetase [Bacillus
UniRef100_P37464
Bacillus subtilis
SerS



subtilis]


15
Glycerate kinase [Bacillus subtilis]
UniRef100_P42100
Bacillus subtilis
YxaA


16
H+/gluconate symporter [Vibrio
UniRef100_Q7MHW6
Vibrio vulnificus
YuiF



vulnificus]


17
Sugar diacid utilization regulator [Vibrio
UniRef100_Q8DBZ9
Vibrio vulnificus
YsfB



vulnificus]










18
Hypothetical protein [Bacillus
UniRef100_Q6HH43
Bacillus thuringiensis



thuringiensis]











19
Hypothetical protein yaaF [Bacillus
UniRef100_P37529
Bacillus subtilis
Dck



subtilis]


20
Hypothetical protein yaaG [Bacillus
UniRef100_P37530
Bacillus subtilis
Dgk



subtilis]


21
Hypothetical protein yaaH [Bacillus
UniRef100_P37531
Bacillus subtilis
YaaH



subtilis]


22
Hypothetical protein yaaI [Bacillus
UniRef100_P37532
Bacillus subtilis
YaaI



subtilis]


23



YaaJ


24



DnaX


25
Hypothetical UPF0133 protein yaaK
UniRef100_P24281
Bacillus subtilis
YaaK



[Bacillus subtilis]


26
Recombination protein recR [Bacillus
UniRef100_P24277
Bacillus subtilis
RecR



subtilis]


27
Hypothetical protein yaaL [Bacillus
UniRef100_P37533
Bacillus subtilis



subtilis]


28
Sigma-K factor processing regulatory
UniRef100_P24282
Bacillus subtilis



protein BOFA [Bacillus subtilis]


29
CsfB protein [Bacillus subtilis]
UniRef100_P37534
Bacillus subtilis


30
XpaC protein [Bacillus subtilis]
UniRef100_P37467
Bacillus subtilis
XpaC


31
Hypothetical protein yaaN [Bacillus
UniRef100_P37535
Bacillus subtilis
YaaN



subtilis]


32



YaaO


33
Thymidylate kinase [Bacillus subtilis]
UniRef100_P37537
Bacillus subtilis
Tmk


34
Hypothetical protein yaaQ [Bacillus
UniRef100_P37538
Bacillus subtilis
YaaQ



subtilis]


35


36
DNA polymerase III, delta' subunit
UniRef100_P37540
Bacillus subtilis
HolB



[Bacillus subtilis]


37
Hypothetical protein yaaT [Bacillus
UniRef100_P37541
Bacillus subtilis
YaaT



subtilis]


38
Hypothetical protein yabA [Bacillus
UniRef100_P37542
Bacillus subtilis
YabA



subtilis]


39
Hypothetical protein yabB [Bacillus
UniRef100_P37543
Bacillus subtilis
YabB



subtilis]


40
Hypothetical UPF0213 protein yazA
UniRef100_O31414
Bacillus subtilis



[Bacillus subtilis]


41
Hypothetical UPF0011 protein yabC
UniRef100_P37544
Bacillus subtilis
YabC



[Bacillus subtilis]


42
Transition state regulatory protein abrB
UniRef100_P08874
Bacillus subtilis



[Bacillus subtilis]


43
Methionyl-tRNA synthetase [Bacillus
UniRef100_P37465
Bacillus subtilis
MetS



subtilis]


44
Putative deoxyribonuclease yabD
UniRef100_P37545
Bacillus subtilis
YabD



[Bacillus subtilis]


45



YabE


46
Hypothetical protein yabF [Bacillus
UniRef100_P37547
Bacillus subtilis
RnmV



subtilis]


47
Dimethyladenosine transferase (EC
UniRef100_P37468
Bacillus subtilis
KsgA



2.1.1.—) (S-adenosylmethionine-6-N′,



N′-adenosyl(rRNA)



dimethyltransferase) [Bacillus subtilis]


48
Hypothetical protein yabG [Bacillus
UniRef100_P37548
Bacillus subtilis
YabG



subtilis]


49
Veg protein [Bacillus subtilis]
UniRef100_P37466
Bacillus subtilis


50
SspF protein [Bacillus subtilis]
UniRef100_P37549
Bacillus subtilis


51



IspE


52
Pur operon repressor [Bacillus subtilis]
UniRef100_P37551
Bacillus subtilis
PurR


53
UPF0076 protein yabJ [Bacillus
UniRef100_P37552
Bacillus subtilis
YabJ



subtilis]


54
Stage V sporulation protein G [Bacillus
UniRef100_P28015
Bacillus subtilis



subtilis]


55
Bifunctional gcaD protein (TMS protein)
UniRef100_P14192
Includes: UDP-
GcaD



[Includes: UDP-N-acetylglucosamine

N-



pyrophosphorylase (EC 2.7.7.23) (N-

acetylglucosamine



acetylglucosamine-1-phosphate

pyrophosphorylase



uridyltransferase); Glucosamine-1-

(EC 2.7.7.23)



phosphate N-acetyltransferase (EC

(N-



2.3.1.157)] [Bacillus subtilis]

acetylglucosamine-





1-phosphate





uridyltransferase);





Glucosamine-





1-phosphate N-





acetyltransferase





(EC 2.3.1.157)


56
Ribose-phosphate pyrophosphokinase
UniRef100_P14193
Bacillus subtilis
Prs



[Bacillus subtilis]


57
General stress protein ctc [Bacillus
UniRef100_P14194
Bacillus subtilis
Ctc



subtilis]


58
Peptidyl-tRNA hydrolase [Bacillus
UniRef100_P37470
Bacillus subtilis
SpoVC



subtilis]


59
Hypothetical protein yabK [Bacillus
UniRef100_P37553
Bacillus subtilis



subtilis]


60
Transcription-repair coupling factor
UniRef100_P37474
Bacillus subtilis
Mfd



[Bacillus subtilis]


61
Stage V sporulation protein T [Bacillus
UniRef100_P37554
Bacillus subtilis
SpoVT



subtilis]


62
Hypothetical protein yabM [Bacillus
UniRef100_P37555
Bacillus subtilis
YabM



subtilis]


63
Hypothetical protein yabN [Bacillus
UniRef100_P37556
Bacillus subtilis
YabN



subtilis]


64
Hypothetical protein yabO [Bacillus
UniRef100_P37557
Bacillus subtilis



subtilis]


65
Hypothetical protein yabP [Bacillus
UniRef100_P37558
Bacillus subtilis
YabP



subtilis]


66
Hypothetical protein yabQ [Bacillus
UniRef100_P37559
Bacillus subtilis
YabQ



subtilis]


67
Cell division protein divlC [Bacillus
UniRef100_P37471
Bacillus subtilis
DivlC



subtilis]


68
Hypothetical protein yabR [Bacillus
UniRef100_P37560
Bacillus subtilis
YabR



subtilis]


69
Stage II sporulation protein E [Bacillus
UniRef100_P37475
Bacillus subtilis
SpoIIE



subtilis]


70
Hypothetical protein yabS [Bacillus
UniRef100_P37561
Bacillus subtilis
YabS



subtilis]


71
Probable serine/threonine-protein
UniRef100_P37562
Bacillus subtilis
YabT



kinase yabT [Bacillus subtilis]


72
Hypothetical UPF0072 protein yacA
UniRef100_P37563
Bacillus subtilis
YacA



[Bacillus subtilis]


73
Hypoxanthine-guanine
UniRef100_P37472
Bacillus subtilis
HprT



phosphoribosyltransferase [Bacillus



subtilis]


74
Cell division protein ftsH homolog
UniRef100_P37476
Bacillus subtilis
FtsH



[Bacillus subtilis]


75
Putative 32 kDa replication protein
UniRef100_Q9F985
Bacillus
YacB



[Bacillus stearothermophilus]

stearothermophilus


76
33 kDa chaperonin [Bacillus subtilis]
UniRef100_P37565
Bacillus subtilis
YacC


77



YacD


78



CysK


79
Para-aminobenzoate synthase
UniRef100_P28820
Bacillus subtilis
PabB



component I [Bacillus subtilis]


80
Para-aminobenzoate/anthranilate
UniRef100_P28819
Includes: Para-
PabA



synthase glutamine amidotransferase

aminobenzoate



component II [Includes: Para-

synthase



aminobenzoate synthase glutamine

glutamine



amidotransferase component II (EC

amidotransferase



6.3.5.8) (ADC synthase); Anthranilate

component II



synthase component II (EC 4.1.3.27)]

(EC 6.3.5.8)



[Bacillus subtil

(ADC synthase);





Anthranilate





synthase





component II





(EC 4.1.3.27)


81
Aminodeoxychorismate lyase [Bacillus
UniRef100_P28821
Bacillus subtilis
PabC



subtilis]


82
Dihydropteroate synthase [Bacillus
UniRef100_P28822
Bacillus subtilis
SuI



subtilis]


83
Dihydroneopterin aldolase [Bacillus
UniRef100_P28823
Bacillus subtilis
FolB



subtilis]


84
2-amino-4-hydroxy-6-
UniRef100_P29252
Bacillus subtilis
FolK



hydroxymethyldihydropteridine



pyrophosphokinase [Bacillus subtilis]


85
YazB protein [Bacillus subtilis]
UniRef100_O31417
Bacillus subtilis


86
Probable tRNA-dihydrouridine
UniRef100_P37567
Bacillus subtilis
YacF



synthase 1 [Bacillus subtilis]


87
Lysyl-tRNA synthetase [Bacillus
UniRef100_P37477
Bacillus subtilis
LysS



subtilis]


88
Transcriptional regulator ctsR [Bacillus
UniRef100_P37568
Bacillus subtilis
CtsR



subtilis]


89
Hypothetical protein yacH [Bacillus
UniRef100_P37569
Bacillus subtilis
McsA



subtilis]


90
Hypothetical ATP: guanido
UniRef100_P37570
Bacillus subtilis
McsB



phosphotransferase yacl [Bacillus



subtilis]


91
Negative regulator of genetic
UniRef100_P37571
Bacillus subtilis
ClpC



competence clpC/mecB [Bacillus



subtilis]


92



RadA


93
Hypothetical protein yacK [Bacillus
UniRef100_P37573
Bacillus subtilis
YacK



subtilis]


94
Hypothetical protein yacL [Bacillus
UniRef100_Q06754
Bacillus subtilis
YacL



subtilis]


95
2-C-methyl-D-erythritol 4-phosphate
UniRef100_Q06755
Bacillus subtilis
YacM



cytidylyltransferase [Bacillus subtilis]


96
2-C-methyl-D-erythritol 2,4-
UniRef100_Q06756
Bacillus subtilis
YacN



cyclodiphosphate synthase [Bacillus



subtilis]


97
Glutamyl-tRNA synthetase [Bacillus
UniRef100_P22250
Bacillus subtilis
GltX



subtilis]


98
Serine acetyltransferase [Bacillus
UniRef100_Q06750
Bacillus subtilis
CysE



subtilis]


99
Cysteinyl-tRNA synthetase [Bacillus
UniRef100_Q06752
Bacillus subtilis
CysS



subtilis]


100
YazC protein [Bacillus subtilis]
UniRef100_O31418
Bacillus subtilis
YazC


101
Hypothetical tRNA/rRNA
UniRef100_Q06753
Bacillus subtilis
YacO



methyltransferase yacO [Bacillus



subtilis]


102
Hypothetical protein yacP [Bacillus
UniRef100_P37574
Bacillus subtilis
YacP



subtilis]


103
RNA polymerase sigma-H factor
UniRef100_P17869
Bacillus subtilis
SigH



[Bacillus subtilis]


104
Preprotein translocase secE subunit
UniRef100_Q06799
Bacillus subtilis



[Bacillus subtilis]


105



NusG


106
50S ribosomal protein L11 [Bacillus
UniRef100_Q06796
Bacillus subtilis
RplK



subtilis]


107
50S ribosomal protein L1 [Bacillus
UniRef100_Q06797
Bacillus subtilis
RplA



subtilis]


108
50S ribosomal protein L10 [Bacillus
UniRef100_P42923
Bacillus subtilis
RplJ



subtilis]


109
50S ribosomal protein L7/L12 [Bacillus
UniRef100_P02394
Bacillus subtilis
RplL



subtilis]


110
Hypothetical protein ybxB [Bacillus
UniRef100_P37872
Bacillus subtilis
YbxB



subtilis]


111
DNA-directed RNA polymerase beta
UniRef100_P37870
Bacillus subtilis
RpoB



chain [Bacillus subtilis]


112
DNA-directed RNA polymerase beta′
UniRef100_P37871
Bacillus subtilis
RpoC



chain [Bacillus subtilis]


113
Putative ribosomal protein L7Ae-like
UniRef100_P46350
Bacillus subtilis



[Bacillus subtilis]


114
30S ribosomal protein S12 [Bacillus
UniRef100_P21472
Bacillus subtilis
RpsL



subtilis]


115
30S ribosomal protein S7 [Bacillus
UniRef100_P21469
Bacillus subtilis
RpsG



subtilis]


116
Elongation factor G [Bacillus subtilis]
UniRef100_P80868
Bacillus subtilis
FusA


117
Elongation factor Tu [Bacillus subtilis]
UniRef100_P33166
Bacillus subtilis
TufA


118
30S ribosomal protein S10 [Bacillus
UniRef100_Q9Z9L5
Bacillus
RpsJ



halodurans]

halodurans


119
50S ribosomal protein L3 [Bacillus
UniRef100_P42920
Bacillus subtilis
RplC



subtilis]


120
50S ribosomal protein L4 [Bacillus
UniRef100_P42921
Bacillus subtilis
RplD



subtilis]


121
50S ribosomal protein L23 [Bacillus
UniRef100_P42924
Bacillus subtilis



subtilis]


122
50S ribosomal protein L2 [Bacillus
UniRef100_P42919
Bacillus subtilis
RplB



subtilis]


123
30S ribosomal protein S19 [Bacillus
UniRef100_P21476
Bacillus subtilis



subtilis]


124
50S ribosomal protein L22 [Bacillus
UniRef100_P42060
Bacillus subtilis
RplV



subtilis]


125
30S ribosomal protein S3 [Bacillus
UniRef100_P21465
Bacillus subtilis
RpsC



subtilis]


126
50S ribosomal protein L16 [Bacillus
UniRef100_P14577
Bacillus subtilis
RplP



subtilis]


127
50S ribosomal protein L29 [Bacillus
UniRef100_P12873
Bacillus subtilis



subtilis]


128
30S ribosomal protein S17 [Bacillus
UniRef100_P12874
Bacillus subtilis



subtilis]


129


130
50S ribosomal protein L24 [Bacillus
UniRef100_P12876
Bacillus subtilis
RplX



subtilis]


131
50S ribosomal protein L5 [Bacillus
UniRef100_P12877
Bacillus subtilis
RplE



subtilis]










132
Ribosomal protein S14 [Bacillus cereus
UniRef100_Q63H77
Bacillus cereus ZK



ZK]











133
30S ribosomal protein S8 [Bacillus
UniRef100_P12879
Bacillus subtilis
RpsH



subtilis]


134
50S ribosomal protein L6 [Bacillus
UniRef100_P46898
Bacillus subtilis
RplF



subtilis]


135
50S ribosomal protein L18 [Bacillus
UniRef100_P46899
Bacillus subtilis
RplR



subtilis]


136
30S ribosomal protein S5 [Bacillus
UniRef100_P21467
Bacillus subtilis
RpsE



subtilis]


137
50S ribosomal protein L30 [Bacillus
UniRef100_P19947
Bacillus subtilis



subtilis]


138
50S ribosomal protein L15 [Bacillus
UniRef100_P19946
Bacillus subtilis
RplO



subtilis]


139



SecY


140
Adenylate kinase [Bacillus subtilis]
UniRef100_P16304
Bacillus subtilis
Adk


141
Methionine aminopeptidase [Bacillus
UniRef100_P19994
Bacillus subtilis
Map



subtilis]










142
C-125 initiation factor IF-I, RNA
UniRef100_O50629
Bacillus halodurans



polymerase alpha subunit and



ribosomal proteins, partial and



complete cds [Bacillus halodurans]











143
Translation initiation factor IF-1
UniRef100_P20458
Bacillus subtilis




[Bacillus subtilis]


144


145



RpsM


146
30S ribosomal protein S11 [Bacillus
UniRef100_P04969
Bacillus subtilis
RpsK



subtilis]


147
DNA-directed RNA polymerase alpha
UniRef100_P20429
Bacillus subtilis
RpoA



chain [Bacillus subtilis]


148
50S ribosomal protein L17 [Bacillus
UniRef100_P20277
Bacillus subtilis
RplQ



subtilis]


149
Hypothetical ABC transporter ATP-
UniRef100_P40735
Bacillus subtilis
YbxA



binding protein ybxA [Bacillus subtilis]


150
Hypothetical protein orf5 [Bacillus
UniRef100_P70970
Bacillus subtilis
YbaE



subtilis]


151
YbaF protein [Bacillus subtilis]
UniRef100_P70972
Bacillus subtilis
YbaF


152
tRNA pseudouridine synthase A
UniRef100_P70973
Bacillus subtilis
TruA



[Bacillus subtilis]


153
50S ribosomal protein L13 [Bacillus
UniRef100_P70974
Bacillus subtilis
RplM



subtilis]


154
30S ribosomal protein S9 [Bacillus
UniRef100_P21470
Bacillus subtilis
RpsI



subtilis]


155
Hypothetical protein [Bacillus cereus]
UniRef100_Q737T6
Bacillus cereus
YizA


156
Hypothetical protein ybaK [Bacillus
UniRef100_P50862
Bacillus subtilis
YbaK



subtilis]


157
Germination-specific N-
UniRef100_P50864
Bacillus subtilis
CwlD



acetylmuramoyl-L-alanine amidase



[Bacillus subtilis]


158



YbaL


159
Spore germination protein gerD
UniRef100_P16450
Bacillus subtilis
GerD



precursor [Bacillus subtilis]


160
KinB signaling pathway activation
UniRef100_P16449
Bacillus subtilis
KbaA



protein [Bacillus subtilis]


161
Hypothetical protein ybaN precursor
UniRef100_P50865
Bacillus subtilis
YbaN



[Bacillus subtilis]


162
Penicillin-binding protein [Bacillus
UniRef100_O31773
Bacillus subtilis
PbpX



subtilis]


163


164
Hypothetical protein ybaS [Bacillus
UniRef100_P55190
Bacillus subtilis
YbaS



subtilis]


165
Hypothetical protein yxaJ [Bacillus
UniRef100_P42109
Bacillus subtilis
YxaJ



subtilis]


166
Phenazine biosynthetic protein
UniRef100_Q9HHG6
Halobacterium
YfhB



[Halobacterium sp.]

sp.


167
Hypothetical protein ybbC precursor
UniRef100_P40407
Bacillus subtilis
YbbC



[Bacillus subtilis]


168
Hypothetical lipoprotein ybbD precursor
UniRef100_P40406
Bacillus subtilis
YbbD



[Bacillus subtilis]


169
Hypothetical UPF0214 protein ybbE
UniRef100_O05213
Bacillus subtilis
YbbE



precursor [Bacillus subtilis]


170
YbbF protein [Bacillus subtilis]
UniRef100_Q797S1
Bacillus subtilis
YbbF


171
Putative HTH-type transcriptional
UniRef100_Q45581
Bacillus subtilis
YbbH



regulator ybbH [Bacillus subtilis]


172
Hypothetical protein ybbI [Bacillus
UniRef100_Q45582
Bacillus subtilis
YbbI



subtilis]


173
Hypothetical protein ybbK [Bacillus
UniRef100_Q45584
Bacillus subtilis
YbbK



subtilis]


174
Arginase [Bacillus caldovelox]
UniRef100_P53608
Bacillus
RocF





caldovelox


175
RNA polymerase sigma factor sigW
UniRef100_Q45585
Bacillus subtilis
SigW



[Bacillus subtilis]


176
YbbM protein [Bacillus subtilis]
UniRef100_Q45588
Bacillus subtilis
YbbM


177
YbbP protein [Bacillus subtilis]
UniRef100_Q45589
Bacillus subtilis
YbbP


178
YbbR protein [Bacillus subtilis]
UniRef100_O34659
Bacillus subtilis
YbbR


179
YbbT protein [Bacillus subtilis]
UniRef100_O34824
Bacillus subtilis
YbbT


180
Glucosamine--fructose-6-phosphate
UniRef100_P39754
isomerizing
GlmS



aminotransferase [isomerizing]



[Bacillus subtilis]


181










182
Hypothetical protein [Bacillus
UniRef100_Q6HH47
Bacillus thuringiensis



thuringiensis]










183
UPI00003CBF92 UniRef100 entry
UniRef100_UPI00003CBF92
YtrB











184
Transcriptional regulator [Bacillus
UniRef100_Q9KF35
Bacillus
YtrA



halodurans]

halodurans


185


186
YbcL protein [Bacillus subtilis]
UniRef100_O34663
Bacillus subtilis
YbcL










187
BH0186 protein [Bacillus halodurans]
UniRef100_Q9KGB8
Bacillus halodurans











188
ABC transporter [Bacillus halodurans]
UniRef100_Q9KEY6
Bacillus
YvcC





halodurans


189
Hypothetical protein ywbO [Bacillus
UniRef100_P39598
Bacillus subtilis
YwbO



subtilis]


190










191
BH0695 protein [Bacillus halodurans]
UniRef100_Q9KF04
Bacillus halodurans











192
Aminoglycoside 6-adenylyltranserase
UniRef100_Q6V4U6
Enterococcus
AadK



[Enterococcus faecium]

faecium










193
Hypothetical conserved protein
UniRef100_Q8ERX2
Oceanobacillus iheyensis



[Oceanobacillus iheyensis]











194
YfnB [Bacillus subtilis]
UniRef100_O06480
Bacillus subtilis
YfnB


195
Hypothetical transport protein ybxG
UniRef100_P54425
Bacillus subtilis
YbxG



[Bacillus subtilis]


196
Mg(2+)/citrate complex secondary
UniRef100_P55069
Bacillus subtilis
CitM



transporter [Bacillus subtilis]


197
YflP protein [Bacillus subtilis]
UniRef100_O034439
Bacillus subtilis
YflP


198



CitT


199
Sensor protein citS [Bacillus subtilis]
UniRef100_O34427
Bacillus subtilis
CitS


200
Transcriptional regulator
UniRef100_Q8RB37
Thermoanaerobacter
YwfK



[Thermoanaerobacter tengcongensis]

tengcongensis


201
Complete genome; segment 8/17
UniRef100_Q7N4W7
Photorhabdus
YwfE



[Photorhabdus luminescens]

luminescens


202
Multidrug resistance protein B [Bacillus
UniRef100_Q81AF0
Bacillus cereus
YqjV



cereus]


203
Sigma-G-dependent sporulation
UniRef100_P54379
Bacillus subtilis



specific SASP protein [Bacillus subtilis]


204
Hypothetical protein ybxH [Bacillus
UniRef100_P54426
Bacillus subtilis



subtilis]


205
YbyB protein [Bacillus subtilis]
UniRef100_O31441
Bacillus subtilis


206
Hypothetical protein yyaL [Bacillus
UniRef100_P37512
Bacillus subtilis
YyaL



subtilis]


207
Hypothetical protein yyaO [Bacillus
UniRef100_P37509
Bacillus subtilis



subtilis]


208
Threonyl-tRNA synthetase 2 [Bacillus
UniRef100_P18256
Bacillus subtilis
ThrZ



subtilis]


209


210



YttB










211
Hypothetical protein [Acinetobacter sp.]
UniRef100_Q6FDN0
Acinetobacter sp.











212
Hypothetical UPF0053 protein yrkA
UniRef100_P54428
Bacillus subtilis
YrkA



[Bacillus subtilis]


213



YdeG


214



YbaJ


215


216



YqeW


217
YubF protein [Bacillus subtilis]
UniRef100_O32082
Bacillus subtilis


218



YbfF


219
Hypothetical protein [Bacteroides
UniRef100_Q64UG6
Bacteroides fragilis



fragilis]


220
Hypothetical transport protein ybhF
UniRef100_O31448
Bacillus subtilis
YbfH



[Bacillus subtilis]


221
YbfI protein [Bacillus subtilis]
UniRef100_O31449
Bacillus subtilis
YbfI


222










223
UPI00003CBC98 UniRef100 entry
UniRef100_UPI00003CBC98



224










225
Oxidoreductase [Lactococcus lactis]
UniRef100_Q9CFC5
Lactococcus lactis











226
Methyl-accepting chemotaxis protein
UniRef100_Q8EST3
Oceanobacillus
YvaQ



[Oceanobacillus iheyensis]

iheyensis


227
D-xylose-binding protein
UniRef100_O68456
Thermoanaerobacter
RbsB



[Thermoanaerobacter ethanolicus]

ethanolicus


228
Hypothetical protein OB0544
UniRef100_Q8ESS4
Oceanobacillus
YomI



[Oceanobacillus iheyensis]

iheyensis


229
Hypothetical protein [Bacillus cereus]
UniRef100_Q81IR4
Bacillus cereus










230
Streptogramin B lactonase
UniRef100_O87275
Staphylococcus cohnii



[Staphylococcus cohnii]











231
Hypothetical protein ycbP [Bacillus
UniRef100_P42248
Bacillus subtilis
YcbP



subtilis]


232
YbgF protein [Bacillus subtilis]
UniRef100_O31462
Bacillus subtilis
YbgF


233
YbgG protein [Bacillus subtilis]
UniRef100_O31463
Bacillus subtilis
YbgG


234


235
PTS system, n-acetylglucosamine-
UniRef100_Q9KF24
Bacillus
NagP



specific enzyme II, ABC component

halodurans



[Bacillus halodurans]










236
UPI00003CBDBC UniRef100 entry
UniRef100_UPI00003CBDBC
Mta











237
Hypothetical protein [Bacillus
UniRef100_Q6HIW0
Bacillus
YdfS



thuringiensis]

thuringiensis


238
Carbonic anhydrase [Methanosarcina
UniRef100_Q8PSJ1
Methanosarcina
YtoA



mazei]

mazei


239
Hypothetical protein yjgA [Bacillus
UniRef100_Q6HHU2
Bacillus
YjgA



thuringiensis]

thuringiensis


240
Amino acid carrier protein [Bacillus
UniRef100_Q6HGU4
Bacillus
YbgH



thuringiensis]

thuringiensis


241
Probable glutaminase ybgJ [Bacillus
UniRef100_O31465
Bacillus subtilis
YbgJ



subtilis]


242
Two-component sensor kinase ycbA
UniRef100_Q81BN8
Bacillus cereus
YcbA



[Bacillus cereus]


243
Hypothetical sensory transduction
UniRef100_P40759
Bacillus subtilis
YcbB



protein ycbB [Bacillus subtilis]


244



YoaA


245



YbxI


246


247
Beta-lactamase precursor (EC 3.5.2.6)
UniRef100_P00808
Contains: Large
PenP



(Penicillinase) [Contains: Large

exopenicillinase;



exopenicillinase; Small

Small



exopenicillinase] [Bacillus licheniformis]

exopenicillinase


248
Alkaline phosphatase D precursor
UniRef100_P42251
Bacillus subtilis
PhoD



[Bacillus subtilis]


249


250
Hypothetical protein ycbT [Bacillus
UniRef100_P42252
Bacillus subtilis
TatCD



subtilis]


251



YcbC


252
Probable aldehyde dehydrogenase
UniRef100_P42236
Bacillus subtilis
YcbD



ycbD [Bacillus subtilis]


253
Probable glucarate transporter [Bacillus
UniRef100_P42237
Bacillus subtilis
YcbE



subtilis]


254
Probable glucarate dehydratase
UniRef100_P42238
Bacillus subtilis
YcbF



[Bacillus subtilis]


255
Hypothetical transcriptional regulator
UniRef100_P42239
Bacillus subtilis
YcbG



ycbG [Bacillus subtilis]


256
Probable D-galactarate dehydratase
UniRef100_P42240
Bacillus subtilis
YcbH



[Bacillus subtilis]


257
Hypothetical sensory transduction
UniRef100_P42244
Bacillus subtilis
YcbL



protein ycbL [Bacillus subtilis]










258
Sensor histidine kinase [Bacillus
UniRef100_Q633Q6
Bacillus cereus ZK



cereus ZK]











259
Hypothetical ABC transporter ATP-
UniRef100_P42246
Bacillus subtilis
YcbN



binding protein ycbN [Bacillus subtilis]


260
Hypothetical protein ycbO [Bacillus
UniRef100_P42247
Bacillus subtilis
YcbO



subtilis]


261



YcbO


262
YfnK [Bacillus subtilis]
UniRef100_O06490
Bacillus subtilis
YetN


263
Hypothetical protein ybdO [Bacillus
UniRef100_O31437
Bacillus subtilis
YbdO



subtilis]


264
Hypothetical protein ycbJ [Bacillus
UniRef100_P42242
Bacillus subtilis
YcbJ



subtilis]


265
Hypothetical protein ywhA [Bacillus
UniRef100_P70993
Bacillus subtilis
YwhA



subtilis]


266



YdaB


267
AII0778 protein [Anabaena sp.]
UniRef100 Q8YYR8
Anabaena sp.


268
BH1298 protein [Bacillus halodurans]
UniRef100_Q9KDB5
Bacillus
YbdN





halodurans










269
BH1299 protein [Bacillus halodurans]
UniRef100_Q9KDB4
Bacillus halodurans











270
Transcriptional activator tipA, putative
UniRef100_Q81XN3
Bacillus
Mta



[Bacillus anthracis]

anthracis


271


272
Transcriptional regulator, PadR family
UniRef100_Q81BZ0
Bacillus cereus



[Bacillus cereus]










273
Hypothetical protein [Bacillus cereus
UniRef100_Q639U7
Bacillus cereus ZK



ZK]











274
Tryptophan RNA-binding attenuator
UniRef100_O31466
Bacillus subtilis




protein-inhibitory protein [Bacillus



subtilis]


275
Hypothetical transport protein ycbK
UniRef100_P42243
Bacillus subtilis
YcbK



[Bacillus subtilis]


276



YczC


277
Hypothetical protein yccF [Bacillus
UniRef100_O34478
Bacillus subtilis
YccF



subtilis]


278
SpaF [Bacillus subtilis]
UniRef100_Q45404
Bacillus subtilis
YhcH


279
SpaE [Bacillus subtilis]
UniRef100_O52853
Bacillus subtilis


280
Putative SpaG [Bacillus subtilis]
UniRef100_Q93GG9
Bacillus subtilis


281
Subtilin biosynthesis regulatory protein
UniRef100_P33112
Bacillus subtilis
YycF



spaR [Bacillus subtilis]


282
Putative histidine kinase [Bacillus
UniRef100_Q93GG7
Bacillus subtilis
ResE



subtilis]










283
UPI00003CA401 UniRef100 entry
UniRef100_UPI00003CA401
YtlI











284
YusQ protein [Bacillus subtilis]
UniRef100_O32183
Bacillus subtilis
YusQ


285
Complete genome; segment 6/17
UniRef100_Q7N6K9
Photorhabdus
YusR



[Photorhabdus luminescens]

luminescens










286
Methyltransferase [Bacillus
UniRef100_Q6HK82
Bacillus thuringiensis



thuringiensis]











287
Hypothetical lipoprotein ycdA precursor
UniRef100_O34538
Bacillus subtilis
YcdA



[Bacillus subtilis]










288
UPI00003CB481 UniRef100 entry
UniRef100_UPI00003CB481
YcgA











289
Hypothetical conserved protein
UniRef100_Q8ESQ2
Oceanobacillus
YtnL



[Oceanobacillus iheyensis]

iheyensis


290
Hypothetical conserved protein
UniRef100_Q8ET83
Oceanobacillus
YvbK



[Oceanobacillus iheyensis]

iheyensis


291



GatA


292
Oligoendopeptidase F, putative
UniRef100_Q81JJ8
Bacillus
YjbG



[Bacillus anthracis]

anthracis


293










294
UPI00003CC42D UniRef100 entry
UniRef100_UPI00003CC42D
PbpX











295
Hypothetical protein ysfD [Bacillus
UniRef100_P94534
Bacillus subtilis
YsfD



subtilis]


296
Hypothetical protein ysfC [Bacillus
UniRef100_P94535
Bacillus subtilis
YsfC



subtilis]










297
PTS system, cellobiose-specific
UniRef100_Q9KEE3
Bacillus halodurans



enzyme II, B component [Bacillus



halodurans]











298
PTS system, cellobiose-specific
UniRef100_Q9KEE2
Bacillus
LicA



enzyme II, A component [Bacillus

halodurans



halodurans]


299
PTS system, cellobiose-specific
UniRef100_Q8EP43
Oceanobacillus
YwbA



enzyme II, C component

iheyensis



[Oceanobacillus iheyensis]


300
6-phospho-beta-glucosidase [Bacillus
UniRef100_Q9KEE0
Bacillus
LicH



halodurans]

halodurans


301
Transcriptional regulator [Bacillus
UniRef100_Q9KED8
Bacillus
YbgA



halodurans]

halodurans


302


303



YvbX










304
Chitinase precursor [Streptomyces
UniRef100_Q9L3E8
Streptomyces olivaceoviridis



olivaceoviridis]











305
Extracellular metalloprotease precursor
UniRef100_P39790
Bacillus subtilis
Mpr



[Bacillus subtilis]


306
Glucose 1-dehydrogenase II [Bacillus
UniRef100_P80869
Bacillus subtilis
YcdF



subtilis]










307
Hypothetical protein OB0244
UniRef100_Q8ETL6
Oceanobacillus iheyensis



[Oceanobacillus iheyensis]











308
Hypothetical protein ycdC [Bacillus
UniRef100_O34772
Bacillus subtilis
YcdC



subtilis]


309


310
Cell wall hydrolase cwlJ [Bacillus
UniRef100_P42249
Bacillus subtilis
CwlJ



subtilis]


311
Hypothetical protein yceB [Bacillus
UniRef100_O34504
Bacillus subtilis
YceB



subtilis]


312
Hypothetical protein yvcE [Bacillus
UniRef100_P40767
Bacillus subtilis
YvcE



subtilis]


313
ABC transporter, permease [Bacillus
UniRef100_Q637N2
Bacillus cereus
YfiM



cereus ZK]

ZK


314
ABC transporter, ATP-binding protein
UniRef100_Q733P8
Bacillus cereus
YfiL



[Bacillus cereus]










315
UPI00003CC482 UniRef100 entry
UniRef100_UPI00003CC482
YsiA










316
Hypothetical protein OB3113
UniRef100_Q8ELV2
Oceanobacillus iheyensis



[Oceanobacillus iheyensis]


317
BH3953 protein [Bacillus halodurans]
UniRef100_Q9K5Y3
Bacillus halodurans











318
BH3951 protein [Bacillus halodurans]
UniRef100_Q9K5Y4
Bacillus
PadR





halodurans


319
Stress response protein SCP2 [Bacillus
UniRef100_P81100
Bacillus subtilis
YceC



subtilis]


320
General stress protein 16U [Bacillus
UniRef100_P80875
Bacillus subtilis
YceD



subtilis]


321
Hypothetical protein yceE [Bacillus
UniRef100_O34384
Bacillus subtilis
YceE



subtilis]


322
Hypothetical protein yceF [Bacillus
UniRef100_O34447
Bacillus subtilis
YceF



subtilis]


323
Hypothetical protein [Bacillus cereus]
UniRef100_Q72YD0
Bacillus cereus


324
YceG [Bacillus subtilis]
UniRef100_O34809
Bacillus subtilis
YceG


325
Hypothetical protein yceH [Bacillus
UniRef100_O34833
Bacillus subtilis
YceH



subtilis]










326
UPI00003CB694 UniRef100 entry
UniRef100_UPI00003CB694
CcdA











327
Hypothetical conserved protein
UniRef100_Q8ELB4
Oceanobacillus
YdiL



[Oceanobacillus iheyensis]

iheyensis


328



Mta


329
Nitrate transporter [Bacillus subtilis]
UniRef100_P42432
Bacillus subtilis
NasA


330
L-lactate dehydrogenase [Bacillus
UniRef100_P13714
Bacillus subtilis
Ldh



subtilis]


331
L-lactate permease [Bacillus subtilis]
UniRef100_P55910
Bacillus subtilis
LctP


332
YcgF protein [Bacillus subtilis]
UniRef100_P94381
Bacillus subtilis
YcgF


333
Homologue of aromatic amino acids
UniRef100_P94383
Bacillus subtilis
YcgH



transport protein of E. coli [Bacillus



subtilis]


334
NH(3)-dependent NAD(+) synthetase
UniRef100_P08164
Bacillus subtilis
NadE



[Bacillus subtilis]


335
Shikimate kinase [Bacillus subtilis]
UniRef100_P37944
Bacillus subtilis
AroK


336
YcgL protein [Bacillus subtilis]
UniRef100_P94389
Bacillus subtilis
YcgL


337
Proline dehydrogenase [Bacillus
UniRef100_Q8RL79
Bacillus subtilis
YcgM



subtilis]


338
1-pyrroline-5-carboxylate
UniRef100_P94391
Bacillus subtilis
YcgN



dehydrogenase 2 [Bacillus subtilis]


339
Homologue of proline permease of E. coli
UniRef100_P94392
Bacillus subtilis
YcgO



[Bacillus subtilis]


340
Hypothetical protein ycgP [Bacillus
UniRef100_P94393
Bacillus subtilis
YcgP



subtilis]


341
YcgQ protein [Bacillus subtilis]
UniRef100_P94394
Bacillus subtilis
YcgQ


342



YcgR


343
Cephalosporin-C deacetylase [Bacillus
UniRef100_Q59233
Bacillus subtilis
Cah



subtilis]


344
Transcriptional regulator [Bacillus
UniRef100_Q9KF41
Bacillus
YdgG



halodurans]

halodurans


345


346


347
Probable amino-acid ABC transporter
UniRef100_P42399
Bacillus subtilis
YckA



permease protein yckA [Bacillus



subtilis]


348
Probable ABC transporter extracellular
UniRef100_P42400
Bacillus subtilis
YckB



binding protein yckB precursor [Bacillus



subtilis]


349
NAD(P)H dehydrogenase (Quinone);
UniRef100_Q63FG6
Bacillus cereus
YwrO



possible modulator of drug activity B

ZK



[Bacillus cereus ZK]










350
BH0315 protein [Bacillus halodurans]
UniRef100_Q9KG01
Bacillus halodurans











351
Probable beta-glucosidase [Bacillus
UniRef100_P42403
Bacillus subtilis
YckE



subtilis]


352
Nin [Bacillus amyloliquefaciens]
UniRef100_Q70KK3
Bacillus
Nin





amyloliquefaciens


353
DNA-entry nuclease [Bacillus subtilis]
UniRef100_P12667
Bacillus subtilis
NucA


354



YbdM


355
Methyl-accepting chemotaxis protein
UniRef100_P39209
Bacillus subtilis
TlpC



tlpC [Bacillus subtilis]


356
Hypothetical protein yqcG [Bacillus
UniRef100_P45942
Bacillus subtilis
YqcG



subtilis]










357
Hydantoin utilization protein A
UniRef100_Q7WCS3
Bordetella bronchiseptica



[Bordetella bronchiseptica]











358
Hydantoin utilization protein B
UniRef100_Q987J6
Rhizobium loti




[Rhizobium loti]










359
UPI000027D233 UniRef100 entry
UniRef100_UPI000027D233
YvfK











360
358aa long hypothetical transporter
UniRef100_Q9YB65
Aeropyrum
MsmX



ATP-binding protein [Aeropyrum pernix]

pernix


361
AttA2-like ABC transporter, permease
UniRef100_Q92ZH0
Rhizobium
YesP



protein [Rhizobium meliloti]

meliloti


362



YurM


363



SrfAA


364



SrfAB


365



SrfAC


366



SrfAD


367



YcxC


368



YcxD


369
4′-phosphopantetheinyl transferase sfp
UniRef100_P39135
Bacillus subtilis
Sfp



[Bacillus subtilis]


370
Predicted esterase of alpha/beta
UniRef100_Q97HP7
Clostridium
YbbA



hydrolase superfamily, YBBA B. subtilis

acetobutylicum



ortholog [Clostridium acetobutylicum]


371
Transcriptional regulator [Clostridium
UniRef100_Q97LX8
Clostridium
YdeE



acetobutylicum]

acetobutylicum


372
YczE [Bacillus subtilis]
UniRef100_Q9F4F8
Bacillus subtilis
YczE










373
Hypothetical protein [Symbiobacterium
UniRef100_Q67MZ7
Symbiobacterium



thermophilum]

thermophilum









374

YfiL


375










376
Hypothetical protein [Symbiobacterium
UniRef100_Q67MZ9
Symbiobacterium



thermophilum]

thermophilum


377


378











379
Methyl-accepting chemotaxis protein
UniRef100_Q6HJV7
Bacillus
TlpB



[Bacillus thuringiensis]

thuringiensis


380
YckI [Bacillus subtilis]
UniRef100_Q9F4F9
Bacillus subtilis
YckI


381
YckJ [Bacillus subtilis]
UniRef100_Q9F4G0
Bacillus subtilis
YckJ


382



YckK


383



RocR


384
Ornithine aminotransferase [Bacillus
UniRef100_P38021
Bacillus subtilis
RocD



subtilis]


385
Amino-acid permease rocE [Bacillus
UniRef100_P39137
Bacillus subtilis
RocE



subtilis]


386
Arginase [Bacillus subtilis]
UniRef100_P39138
Bacillus subtilis
RocF


387
Homologue of als operon regulatory
UniRef100_P94403
Bacillus subtilis
YclA



protein AlsR of B. subtilis [Bacillus



subtilis]


388
Probable aromatic acid decarboxylase
UniRef100_P94404
Bacillus subtilis
YclB



[Bacillus subtilis]


389
Hypothetical protein yclC [Bacillus
UniRef100_P94405
Bacillus subtilis
YclC



subtilis]


390
YclD protein [Bacillus subtilis]
UniRef100_P94406
Bacillus subtilis
YclD


391


392


393










394
Hypothetical protein [Bacillus
UniRef100_Q6HMQ9
Bacillus thuringiensis



thuringiensis]


395
Hypothetical protein OB2810
UniRef100_Q8EMN2
Oceanobacillus iheyensis



[Oceanobacillus iheyensis]


396











397



YxiD


398


399



YxiB


400
Sugar ABC transporter [Bacillus
UniRef100_Q9K7B8
Bacillus
RbsB



halodurans]

halodurans


401
Two-component sensor histidine
UniRef100_Q9K7B9
Bacillus
YesM



kinase [Bacillus halodurans]

halodurans


402



YesN


403
Multiple sugar transport system
UniRef100_Q9K7C2
Bacillus
RbsB



[Bacillus halodurans]

halodurans


404
L-arabinose transport ATP-binding
UniRef100_Q9S472
Bacillus
RbsA



protein araG [Bacillus

stearothermophilus



stearothermophilus]


405
L-arabinose membrane permease
UniRef100_Q9S471
Bacillus
RbsC



[Bacillus stearothermophilus]

stearothermophilus


406
Transporter [Bacillus halodurans]
UniRef100_Q9K9F3
Bacillus
YbfB





halodurans


407
YbfA protein [Bacillus subtilis]
UniRef100_O31443
Bacillus subtilis
YbfA


408
Beta-D-galactosidase [Bacillus
UniRef100_Q45093
Bacillus
LacA



circulans]

circulans


409



Phy


410
Hypothetical transporter yclF [Bacillus
UniRef100_P94408
Bacillus subtilis
YclF



subtilis]


411
YclG protein [Bacillus subtilis]
UniRef100_P94409
Bacillus subtilis
YclG


412


413



GerKA


414
Spore germination protein KC
UniRef100_P49941
Bacillus subtilis
GerKC



precursor [Bacillus subtilis]


415
Spore germination protein KB [Bacillus
UniRef100_P49940
Bacillus subtilis
GerKB



subtilis]


416



Mta


417
Hypothetical protein yclH [Bacillus
UniRef100_P94411
Bacillus subtilis
YclH



subtilis]


418
Hypothetical protein yclI [Bacillus
UniRef100_P94412
Bacillus subtilis
YclI



subtilis]


419
Hypothetical sensory transduction
UniRef100_P94413
Bacillus subtilis
YclJ



protein yclJ [Bacillus subtilis]


420
Hypothetical sensor-like histidine
UniRef100_P94414
Bacillus subtilis
YclK



kinase yclK [Bacillus subtilis]


421
Methyl-accepting chemotaxis protein
UniRef100_Q9K632
Bacillus
TlpA



[Bacillus halodurans]

halodurans


422
Probable aspartokinase [Bacillus
UniRef100_P94417
Bacillus subtilis
YclM



subtilis]


423
Homologue of ferric anguibactin
UniRef100_P94418
Bacillus subtilis
YclN



transport system permerase protein



FatD of V. anguillarum [Bacillus



subtilis]


424
Homologue of ferric anguibactin
UniRef100_P94419
Bacillus subtilis
YclO



transport system permerase protein



FatC of V. anguillarum [Bacillus



subtilis]


425
Homologue of iron dicitrate transport
UniRef100_P94420
Bacillus subtilis
YclP



ATP-binding protein FecE of E. coli



[Bacillus subtilis]


426
Ferric anguibactin-binding protein
UniRef100_P94421
Bacillus subtilis
YclQ



precusor FatB of V. anguillarum



[Bacillus subtilis]


427
Homologue of multidrug resistance
UniRef100_P94422
Bacillus subtilis
YcnB



protein B, EmrB, of E. coli [Bacillus



subtilis]


428
YcnC protein [Bacillus subtilis]
UniRef100_P94423
Bacillus subtilis
YcnC


429
Hypothetical protein [Bacillus cereus
UniRef100_Q636R0
Bacillus cereus
YqiQ



ZK]

ZK


430
Hypothetical oxidoreductase ycnD
UniRef100_P94424
Bacillus subtilis
YcnD



[Bacillus subtilis]


431
Hypothetical protein ycnE [Bacillus
UniRef100_P94425
Bacillus subtilis



subtilis]


432
YczG protein [Bacillus subtilis]
UniRef100_O31480
Bacillus subtilis


433
Homologue of regulatory protein MocR
UniRef100_P94426
Bacillus subtilis
GabR



of R. meliloti [Bacillus subtilis]


434
Probable 4-aminobutyrate
UniRef100_P94427
Bacillus subtilis
GabT



aminotransferase (EC 2.6.1.19) ((S)-3-



amino-2-methylpropionate



transaminase) [Bacillus subtilis]


435
Cationic amino acid transporter
UniRef100_Q8ESX7
Oceanobacillus
YhdG



[Oceanobacillus iheyensis]

iheyensis


436
Homologue of succinate semialdehyde
UniRef100_P94428
Bacillus subtilis
GabD



dehydrogenase GabD of E. coli



[Bacillus subtilis]


437



YwfM


438


439
YcnI protein [Bacillus subtilis]
UniRef100_P94431
Bacillus subtilis
YcnI


440
Homologue of copper export protein
UniRef100_P94432
Bacillus subtilis
YcnJ



PcoD of E. coli [Bacillus subtilis]


441
YcnK protein [Bacillus subtilis]
UniRef100_P94433
Bacillus subtilis
YcnK


442
Assimilatory nitrate reductase electron
UniRef100_P42433
Bacillus subtilis
NasB



transfer subunit [Bacillus subtilis]


443
Assimilatory nitrate reductase catalytic
UniRef100_P42434
Bacillus subtilis
NasC



subunit [Bacillus subtilis]


444
Nitrite reductase [NAD(P)H] [Bacillus
UniRef100_P42435
NAD(P)H
NasD



subtilis]


445
Assimilatory nitrite reductase
UniRef100_P42436
NAD(P)H
NasE



[NAD(P)H] small subunit [Bacillus



subtilis]


446
Uroporphyrin-III C-methyltransferase
UniRef100_P42437
Bacillus subtilis
NasF



[Bacillus subtilis]


447
Hypothetical transcriptional regulator
UniRef100_O05494
Bacillus subtilis
YdhC



ydhC [Bacillus subtilis]


448
Sodium-dependent transporter
UniRef100_Q8ENE3
Oceanobacillus
YflS



[Oceanobacillus iheyensis]

iheyensis


449
Hypothetical protein ycsD [Bacillus
UniRef100_P42961
Bacillus subtilis



subtilis]










450
UPI00003CC424 UniRef100 entry
UniRef100_UPI00003CC424












451
Possible transcriptional antiterminator,
UniRef100_Q63A16
Bacillus cereus
MtlR



bglG family [Bacillus cereus ZK]

ZK










452
Putative sugar-specific PTS component
UniRef100_Q7X1N9
Lactococcus raffinolactis



EIIB [Lactococcus raffinolactis]


453
SgaT protein [Mannheimia
UniRef100_Q65WA2
Mannheimia



succiniciproducens MBEL55E]

succiniciproducens MBEL55E











454
Transketolase, N-terminal subunit
UniRef100_Q8E202
Streptococcus
Tkt



[Streptococcus agalactiae]

agalactiae


455
Putative transketolase [Salmonella
UniRef100_Q8ZND5
Salmonella
Dxs



typhimurium]

typhimurium


456
Hypothetical protein ycsE [Bacillus
UniRef100_P42962
Bacillus subtilis
YcsE



subtilis]


457
Hypothetical UPF0271 protein ycsF
UniRef100_P42963
Bacillus subtilis
YcsF



[Bacillus subtilis]


458
Hypothetical protein ycsG [Bacillus
UniRef100_P42964
Bacillus subtilis
YcsG



subtilis]


459
Hypothetical UPF0317 protein ycsI
UniRef100_P42966
Bacillus subtilis
YcsI



[Bacillus subtilis]


460
Kinase A inhibitor [Bacillus subtilis]
UniRef100_P60495
Bacillus subtilis
KipI


461
KipI antagonist [Bacillus subtilis]
UniRef100_Q7WY77
Bacillus subtilis
KipA


462
HTH-type transcriptional regulator kipR
UniRef100_P42968
Bacillus subtilis
KipR



[Bacillus subtilis]


463
Hypothetical protein ycsK [Bacillus
UniRef100_P42969
Bacillus subtilis
YcsK



subtilis]


464
PTS system, mannitol-specific IIABC
UniRef100_P42956
Bacillus subtilis
MtlA



component [Bacillus subtilis]


465
Mannitol-1-phosphate 5-
UniRef100_P42957
Bacillus subtilis
MtlD



dehydrogenase [Bacillus subtilis]


466
YdaA protein [Bacillus subtilis]
UniRef100_P96574
Bacillus subtilis
MtlR


467
General stress protein 39 [Bacillus
UniRef100_P80873
Bacillus subtilis
YdaD



subtilis]


468
Hypothetical protein ydaE [Bacillus
UniRef100_P96578
Bacillus subtilis
YdaE



subtilis]










469
Hypothetical protein [Bacillus anthracis]
UniRef100_Q81U55
Bacillus anthracis











470
General stress protein 26 [Bacillus
UniRef100_P80238
Bacillus subtilis
YdaG



subtilis]


471
YdaH protein [Bacillus subtilis]
UniRef100_P96581
Bacillus subtilis
YdaH


472
Lin0463 protein [Listeria innocua]
UniRef100_Q92EJ6
Listeria innocua
YvhJ


473
YdzA protein [Bacillus subtilis]
UniRef100_O31485
Bacillus subtilis










474
BH0424 protein [Bacillus halodurans]
UniRef100_Q9KFQ4
Bacillus halodurans











475
HTH-type transcriptional regulator lrpC
UniRef100_P96582
Bacillus subtilis
LrpC



[Bacillus subtilis]


476
PROBABLE DNA TOPOISOMERASE
UniRef100_P96583
Bacillus subtilis
TopB



III [Bacillus subtilis]


477


478


479


480



YdaO


481



YdaO


482
YdaP protein [Bacillus subtilis]
UniRef100_P96591
Bacillus subtilis
YdaP


483










484
UPI00003CC069 UniRef100 entry
UniRef100_UPI00003CC069











485
IS1627s1-related, transposase
UniRef100_Q7CMD0
Bacillus anthracis str. A2012



[Bacillus anthracis str. A2012]


486











487
Similar to ribosomal-protein-serine N-
UniRef100_Q99WN5
Staphylococcus
YdaF



acetyltransferase [Staphylococcus

aureus



aureus]


488
Manganese transport protein mntH
UniRef100_P96593
Bacillus subtilis
MntH



[Bacillus subtilis]


489


490



AnsB


491



YojK


492
YdaT protein [Bacillus subtilis]
UniRef100_P96595
Bacillus subtilis
YdaT


493
Hypothetical protein ydbA [Bacillus
UniRef100_P96596
Bacillus subtilis
YdbA



subtilis]


494
Na+/H+ antiporter NhaC [Bacillus
UniRef100_Q81FX8
Bacillus cereus
NhaC



cereus]


495
YdbB protein [Bacillus subtilis]
UniRef100_P96597
Bacillus subtilis
YdbB


496
Glucose starvation-inducible protein B
UniRef100_P26907
Bacillus subtilis
GsiB



[Bacillus subtilis]


497
Hypothetical UPF0118 protein ydbI
UniRef100_P96604
Bacillus subtilis
YdbI



[Bacillus subtilis]


498



GltT


499
YdbJ protein [Bacillus subtilis]
UniRef100_P96605
Bacillus subtilis
YdbJ


500
YdbK protein [Bacillus subtilis]
UniRef100_P96606
Bacillus subtilis
YdbK


501
Hypothetical protein ydbL [Bacillus
UniRef100_P96607
Bacillus subtilis
YdbL



subtilis]


502
YdbM protein [Bacillus subtilis]
UniRef100_P96608
Bacillus subtilis
YdbM


503


504


505
YdbP protein [Bacillus subtilis]
UniRef100_P96611
Bacillus subtilis
YdbP


506
D-alanine--D-alanine ligase [Bacillus
UniRef100_P96612
Bacillus subtilis
Ddl



subtilis]


507



MurF


508
Esterase [Oceanobacillus iheyensis]
UniRef100_Q8ESM0
Oceanobacillus
YvaK





iheyensis


509
YdbR protein [Bacillus subtilis]
UniRef100_P96614
Bacillus subtilis
YdbR


510
YdbS protein [Bacillus subtilis]
UniRef100_P96615
Bacillus subtilis
YdbS


511
YdbT protein [Bacillus subtilis]
UniRef100_P96616
Bacillus subtilis
YdbT


512
YdcA protein [Bacillus subtilis]
UniRef100_P96617
Bacillus subtilis
YdcA


513



YdcC


514



Alr


515


516
YdcE protein [Bacillus subtilis]
UniRef100_P96622
Bacillus subtilis
YdcE


517



RsbR


518



RsbS


519



RsbT


520



RsbU


521



RsbV


522



RsbW


523



SigB


524



RsbX


525
YdcI protein [Bacillus subtilis]
UniRef100_O31489
Bacillus subtilis
YdcI


526
Transcriptional regulator, TetR family
UniRef100_Q6HGY5
Bacillus
YxbF



[Bacillus thuringiensis]

thuringiensis


527
Lin1189 protein [Listeria innocua]
UniRef100_Q92CI2
Listeria innocua
YdgH


528


529
Protein sprT-like [Bacillus subtilis]
UniRef100_P96628
Bacillus subtilis
YdcK










530
Possible transporter, EamA family
UniRef100_Q638K5
Bacillus cereus ZK



[Bacillus cereus ZK]


531


532
Hypothetical protein ORF00034
UniRef100_O87235
Lactococcus lactis



[Lactococcus lactis]











533
Delta5 acyl-lipid desaturase [Bacillus
UniRef100_Q81C02
Bacillus cereus
Des



cereus]


534
Cold shock protein cspC [Bacillus
UniRef100_P39158
Bacillus subtilis



subtilis]


535



YogA


536
Membrane protein, putative [Bacillus
UniRef100_Q734Y0
Bacillus cereus
YyaS



cereus]


537
Transcriptional regulator, MarR family
UniRef100_Q734X9
Bacillus cereus
YybA



[Bacillus cereus]


538
Acetyltransferase, GNAT family
UniRef100_Q734X8
Bacillus cereus
PaiA



[Bacillus cereus]


539
Protease synthase and sporulation
UniRef100_Q734X7
Bacillus cereus
PaiB



negative regulatory protein PAI 2



[Bacillus cereus]


540
BH0654 protein [Bacillus halodurans]
UniRef100_Q9KF33
Bacillus
RocF





halodurans


541


542
YdeO protein [Bacillus subtilis]
UniRef100_P96672
Bacillus subtilis
YdeO


543
Transporter, LysE family [Bacillus
UniRef100_Q81D17
Bacillus cereus
YrhP



cereus]


544



YwqM


545


546
Permease, putative [Bacillus anthracis]
UniRef100_Q81QY7
Bacillus
YvqJ





anthracis










547
Putative cyclase [Rhodopseudomonas
UniRef100_Q6N497
Rhodopseudomonas palustris



palustris]











548
Hypothetical transport protein ydgF
UniRef100_P96704
Bacillus subtilis
YdgF



[Bacillus subtilis]


549


550



YknW


551
RNA polymerase sigma factor sigV
UniRef100_O05404
Bacillus subtilis
SigV



[Bacillus subtilis]


552
Putative anti-SigV factor [Bacillus
UniRef100_O05403
Bacillus subtilis
YrhM



subtilis]


553
Hypothetical protein yrhL [Bacillus
UniRef100_O05402
Bacillus subtilis
YrhL



subtilis]


554



YdgK


555



YwpD


556



LytT










557
Collagen adhesion protein [Bacillus
UniRef100_Q630P2
Bacillus cereus ZK



cereus ZK]











558
Lin0929 protein [Listeria innocua]
UniRef100_Q92D88
Listeria innocua



559


560
Metabolite transport protein [Bacillus
UniRef100_O34718
Bacillus subtilis
YdjK



subtilis]


561
Thiamine-monophosphate kinase
UniRef100_O05514
Bacillus subtilis
ThiL



[Bacillus subtilis]


562
Hypothetical UPF0079 protein ydiB
UniRef100_O05515
Bacillus subtilis
YdiB



[Bacillus subtilis]


563
YdiC protein [Bacillus subtilis]
UniRef100_O05516
Bacillus subtilis
YdiC


564
YdiD protein [Bacillus subtilis]
UniRef100_O005517
Bacillus subtilis
YdiD


565
Probable O-sialoglycoprotein
UniRef100_O05518
Bacillus subtilis
Gcp



endopeptidase [Bacillus subtilis]


566



YdiF


567



YdiF


568
Molybdenum cofactor biosynthesis
UniRef100_O05520
Bacillus subtilis
YdiG



protein C [Bacillus subtilis]


569
Redox-sensing transcriptional
UniRef100_O05521
Bacillus subtilis
YdiH



repressor rex [Bacillus subtilis]










570
Ydil [Bacillus halodurans]
UniRef100_Q9Z9P5
Bacillus halodurans











571



TatCY


572
Hypothetical lipoprotein ydiK precursor
UniRef100_O05524
Bacillus subtilis



[Bacillus subtilis]


573



YdiL


574
10 kDa chaperonin [Bacillus subtilis]
UniRef100_P28599
Bacillus subtilis


575
60 kDa chaperonin [Bacillus subtilis]
UniRef100_P28598
Bacillus subtilis
GroEL


576


577










578
Hypothetical protein yolD
UniRef100_O64030
Bacteriophage SPBc2



[Bacteriophage SPBc2]


579


580











581
YoaR [Bacillus subtilis]
UniRef100_O34611
Bacillus subtilis
YoaR


582
Hypothetical protein yfmQ [Bacillus
UniRef100_O06475
Bacillus subtilis
YfmQ



subtilis]


583


584
YoqW protein [Bacteriophage SPBc2]
UniRef100_O64131
Bacteriophage
YoqW





SPBc2


585
Lin2076 protein [Listeria innocua]
UniRef100_Q92A46
Listeria innocua
YerO


586
PEP synthase [Bacillus subtilis]
UniRef100_O34309
Bacillus subtilis
Pps


587
Hypothetical protein yoaF [Bacillus
UniRef100_O31829
Bacillus subtilis



subtilis]










588
Hypothetical protein [Staphylococcus
UniRef100_Q6GK76
Staphylococcus aureus



aureus]











589
Short-chain dehydrodenase
UniRef100_Q97LM1
Clostridium
DltE



[Clostridium acetobutylicum]

acetobutylicum


590
Type B carboxylesterase [Bacillus sp.
UniRef100_Q9L378
Bacillus sp. BP-7
PnbA



BP-7]


591
Inositol transport protein
UniRef100_Q8E5X2
Oceanobacillus
IolF



[Oceanobacillus iheyensis]

iheyensis


592
Phage shock protein A homolog
UniRef100_P54617
Bacillus subtilis
PspA



[Bacillus subtilis]


593
YdjG protein [Bacillus subtilis]
UniRef100_O34434
Bacillus subtilis
YdjG


594
YdjH protein [Bacillus subtilis]
UniRef100_O35004
Bacillus subtilis
YdjH


595
YdjI protein [Bacillus subtilis]
UniRef100_O34789
Bacillus subtilis
YdjI


596
Putative oxidoreductase
UniRef100_Q67S08
Symbiobacterium
YtmO



[Symbiobacterium thermophilum]

thermophilum


597
YrhO [Bacillus subtilis]
UniRef100_O05405
Bacillus subtilis
YrhO


598



YrhP


599
Helix-turn-helix domain protein
UniRef100_Q73C00
Bacillus cereus



[Bacillus cereus]


600
Stage V sporulation protein E [Bacillus
UniRef100_Q9K7T4
Bacillus
SpoVE



halodurans]

halodurans


601
Stage V sporulation protein E [Bacillus
UniRef100_Q9K7T3
Bacillus
FtsW



halodurans]

halodurans


602


603
Hypothetical protein [Bacillus cereus]
UniRef100_Q72Z89
Bacillus cereus


604
BH1889 protein [Bacillus halodurans]
UniRef100_Q9KBN6
Bacillus
YobV





halodurans


605



YjeA


606



YjeA


607



TreA


608
Putative HTH-type transcriptional
UniRef100_O06987
Bacillus subtilis
YvdE



regulator yvdE [Bacillus subtilis]


609
Hypothetical protein yvdF [Bacillus
UniRef100_O06988
Bacillus subtilis
YvdF



subtilis]


610
Hypothetical protein yvdG [Bacillus
UniRef100_O06989
Bacillus subtilis
YvdG



subtilis]


611
Hypothetical protein yvdH [Bacillus
UniRef100_O06990
Bacillus subtilis
YvdH



subtilis]


612
Hypothetical protein yvdI [Bacillus
UniRef100_O06991
Bacillus subtilis
YvdI



subtilis]


613
Hypothetical protein yvdJ [Bacillus
UniRef100_O06992
Bacillus subtilis
YvdJ



subtilis]


614
Hypothetical glycosyl hydrolase yvdK
UniRef100_O06993
Bacillus subtilis
YvdK



[Bacillus subtilis]


615
Oligo-1,6-glucosidase [Bacillus subtilis]
UniRef100_O06994
Bacillus subtilis
MalL


616
Putative beta-phosphoglucomutase
UniRef100_O06995
Bacillus subtilis
PgcM



[Bacillus subtilis]


617
Tyrosyl-tRNA synthetase 2 [Bacillus
UniRef100_P25151
Bacillus subtilis
TyrZ



subtilis]


618
Putative HTH-type transcriptional
UniRef100_P25150
Bacillus subtilis
YwaE



regulator ywaE [Bacillus subtilis]


619










620
Hypothetical protein SE2399
UniRef100_Q8CQM7
Staphylococcus epidermidis



[Staphylococcus epidermidis]











621
Hypothetical protein ydjM [Bacillus
UniRef100_P40775
Bacillus subtilis
YdjM



subtilis]


622
YdjN protein [Bacillus subtilis]
UniRef100_O34353
Bacillus subtilis
YdjN


623
Hypothetical protein [Bacillus cereus]
UniRef100_Q81AT6
Bacillus cereus
YeaA


624


625
Signal peptidase I [Bacillus cereus]
UniRef100_Q73C25
Bacillus cereus
SipS


626


627
Hypothetical protein yhfK [Bacillus
UniRef100_O07609
Bacillus subtilis
YhfK



subtilis]


628
Transcriptional regulator [Bacillus
UniRef100_Q9K766
Bacillus
YdeE



halodurans]

halodurans


629
Spore coat protein A [Bacillus subtilis]
UniRef100_P07788
Bacillus subtilis
CotA


630



YkrP


631
Extracellular protein [Lactobacillus
UniRef100_Q88T27
Lactobacillus
YcdA



plantarum]

plantarum


632
Hypothetical UPF0018 protein yeaB
UniRef100_P46348
Bacillus subtilis
YeaB



[Bacillus subtilis]


633
YeaC [Bacillus subtilis]
UniRef100_P94474
Bacillus subtilis
YeaC


634
Hypothetical protein [Bacillus cereus]
UniRef100_Q739D8
Bacillus cereus
YeaD


635
YebA [Bacillus subtilis]
UniRef100_P94476
Bacillus subtilis
YebA


636
GMP synthase [glutamine-hydrolyzing]
UniRef100_P29727
glutamine-
GuaA



[Bacillus subtilis]

hydrolyzing


637
Hypoxanthine/guanine permease
UniRef100_O34987
Bacillus subtilis
PbuG



[Bacillus subtilis]


638



YebC


639
Hypothetical UPF0316 protein yebE
UniRef100_O34624
Bacillus subtilis
YebE



[Bacillus subtilis]


640
YebG protein [Bacillus subtilis]
UniRef100_O34700
Bacillus subtilis


641
Phosphoribosylaminoimidazole
UniRef100_P12044
Bacillus subtilis
PurE



carboxylase catalytic subunit [Bacillus



subtilis]


642



PurK


643
Adenylosuccinate lyase [Bacillus
UniRef100_P12047
Bacillus subtilis
PurB



subtilis]


644
Phosphoribosylaminoimidazole-
UniRef100_P12046
Bacillus subtilis
PurC



succinocarboxamide synthase [Bacillus



subtilis]


645
Hypothetical UPF0062 protein yexA
UniRef100_P12049
Bacillus subtilis



[Bacillus subtilis]


646
Phosphoribosylformylglycinamidine
UniRef100_P12041
Bacillus subtilis
PurQ



synthase I [Bacillus subtilis]


647
Phosphoribosylformylglycinamidine
UniRef100_P12042
Bacillus subtilis
PurL



synthase II [Bacillus subtilis]


648
Amidophosphoribosyltransferase
UniRef100_P00497
Bacillus subtilis
PurF



precursor [Bacillus subtilis]


649



PurM


650
Phosphoribosylglycinamide
UniRef100_P12040
Bacillus subtilis
PurN



formyltransferase [Bacillus subtilis]


651
Bifunctional purine biosynthesis protein
UniRef100_P12048
Includes:
PurH



purH [Includes:

Phosphoribosylaminoimidazolecarboxamide



Phosphoribosylaminoimidazolecarboxamide

formyltransferase



formyltransferase (EC 2.1.2.3)

(EC 2.1.2.3)



(AICAR transformylase); IMP

(AICAR



cyclohydrolase (EC 3.5.4.10)

transformylase);



(Inosinicase) (IMP synthetase) (ATIC)]

IMP



[Bacillus subtilis]

cyclohydrolase





(EC 3.5.4.10)





(Inosinicase)





(IMP synthetase)





(ATIC)


652
Phosphoribosylamine--glycine ligase
UniRef100_P12039
Bacillus subtilis
PurD



[Bacillus subtilis]


653



YxbF


654
Putative cytochrome P450 yjiB
UniRef100_O34374
Bacillus subtilis
YjiB



[Bacillus subtilis]


655


656
Hypothetical lipoprotein yybP precursor
UniRef100_P37488
Bacillus subtilis
YybP



[Bacillus subtilis]










657
Transposase [Thermoanaerobacter
UniRef100_Q8RCM3
Thermoanaerobacter



tengcongensis]

tengcongensis


658
Hypothetical protein [Bacillus anthracis]
UniRef100_Q81ZG4
Bacillus anthracis











659
Putative adenine deaminase yerA
UniRef100_O34909
Bacillus subtilis
YerA



[Bacillus subtilis]


660
YerB protein [Bacillus subtilis]
UniRef100_O34968
Bacillus subtilis
YerB


661
YecD [Bacillus subtilis]
UniRef100_Q7BVT7
Bacillus subtilis
YerC


662
PcrB protein homolog [Bacillus subtilis]
UniRef100_O34790
Bacillus subtilis
PcrB


663
ATP-dependent DNA helicase pcrA
UniRef100_O34580
Bacillus subtilis
PcrA



[Bacillus subtilis]


664
DNA ligase [Bacillus subtilis]
UniRef100_O31498
Bacillus subtilis
LigA


665
YerH protein [Bacillus subtilis]
UniRef100_O34629
Bacillus subtilis
YerH


666
BH0586 protein [Bacillus halodurans]
UniRef100_Q9KF99
Bacillus halodurans


667


668










669
BH0589 protein [Bacillus halodurans]
UniRef100_Q9KF96
Bacillus halodurans











670
Phosphotriesterase homology protein
UniRef100_P45548
Escherichia coli




[Escherichia coli]


671
Similar to unknown protein YhfS of
UniRef100_Q7N5F2
Photorhabdus
Csd



Escherichia coli [Photorhabdus

luminescens



luminescens]


672
Phosphopentomutase
UniRef100_Q67S06
Symbiobacterium
Drm



[Symbiobacterium thermophilum]

thermophilum










673
Putative alanine racemase
UniRef100_Q67S05
Symbiobacterium



[Symbiobacterium thermophilum]

thermophilum











674
Glucosamine-6-phosphate deaminase
UniRef100_Q8ESL6
Oceanobacillus
NagB



[Oceanobacillus iheyensis]

iheyensis


675
SapB protein [Bacillus subtilis]
UniRef100_Q45514
Bacillus subtilis
SapB


676



OpuE


677
Glutamyl-tRNA(Gln) amidotransferase
UniRef100_O06492
Bacillus subtilis



subunit C [Bacillus subtilis]


678
Glutamyl-tRNA(Gln) amidotransferase
UniRef100_O06491
Bacillus subtilis
GatA



subunit A [Bacillus subtilis]


679



GatB










680
Hypothetical protein [Bacillus
UniRef100_Q848Y2
Bacillus megaterium



megaterium]


681











682



YdhT


683


684
Putative HTH-type transcriptional
UniRef100_O31500
Bacillus subtilis
YerO



regulator yerO [Bacillus subtilis]


685
Swarming motility protein swrC
UniRef100_O31501
Bacillus subtilis
YerP



[Bacillus subtilis]


686



YjcK


687
Inosine-uridine preferring nucleoside
UniRef100_Q81DM6
Bacillus cereus



hydrolase [Bacillus cereus]


688
YerQ protein [Bacillus subtilis]
UniRef100_O31502
Bacillus subtilis
YerQ


689
Hypothetical RNA methyltransferase
UniRef100_O31503
Bacillus subtilis
YefA



yefA [Bacillus subtilis]


690
Type I restriction-modification system
UniRef100_Q817S1
Bacillus cereus



specificity subunit [Bacillus cereus]


691
Type I restriction-modification system
UniRef100_Q817S2
Bacillus cereus



methylation subunit [Bacillus cereus]










692
Type IC specificity subunit
UniRef100_Q9RNW0
Streptococcus thermophilus



[Streptococcus thermophilus]











693
Type I restriction-modification system
UniRef100_Q817S4
Bacillus cereus




restriction subunit [Bacillus cereus]


694


695
Beta-glucosides PTS, EIIBCA
UniRef100_Q88T54
Lactobacillus
BglP



[Lactobacillus plantarum]

plantarum


696
6-phospho-beta-glucosidase
UniRef100_Q88T55
Lactobacillus
BglH



[Lactobacillus plantarum]

plantarum


697



LicT


698
Response regulator aspartate
UniRef100_O34930
Bacillus subtilis
RapK



phosphatase K [Bacillus subtilis]


699










700
Methyltransferase [Bacillus cereus ZK]
UniRef100_Q639N2
Bacillus cereus ZK











701
Hypothetical UPF0082 protein
UniRef100_P62032
Bacillus cereus
Yeel



BCE0595 [Bacillus cereus]










702
Putative HTH-type transcriptional
UniRef100_O28646
Archaeoglobus fulgidus



regulator AF1627 [Archaeoglobus



fulgidus]


703











704
YfmT [Bacillus subtilis]
UniRef100_O06478
Bacillus subtilis
YfmT


705
YfmS [Bacillus subtilis]
UniRef100_O06477
Bacillus subtilis
YfmS


706



YflS


707
YfmR [Bacillus subtilis]
UniRef100_O06476
Bacillus subtilis
YfmR


708


709
YciA protein [Bacillus subtilis]
UniRef100_P94398
Bacillus subtilis
YciA










710
UPI00002BDF65 UniRef100 entry
UniRef100_UPI00002BDF65
YpdA










711
Ferrusion transporter protein
UniRef100_Q6U5S9
Klebsiella pneumoniae



[Klebsiella pneumoniae]


712











713
YciC protein [Bacillus
UniRef100_Q70KK8
Bacillus
YciC



amyloliquefaciens]

amyloliquefaciens


714



BioW


715
Adenosylmethionine-8-amino-7-
UniRef100_P53555
Bacillus subtilis
BioA



oxononanoate aminotransferase



[Bacillus subtilis]


716
8-amino-7-oxononanoate synthase
UniRef100_P53556
Bacillus subtilis
BioF



[Bacillus subtilis]


717
BioD protein [Bacillus
UniRef100_Q70JZ0
Bacillus
BioD



amyloliquefaciens]

amyloliquefaciens


718
BioB protein [Bacillus
UniRef100_Q70JZ1
Bacillus
BioB



amyloliquefaciens]

amyloliquefaciens


719
Biol protein [Bacillus
UniRef100_Q70JZ2
Bacillus
BioI



amyloliquefaciens]

amyloliquefaciens










720
BH1501 protein [Bacillus halodurans]
UniRef100_Q9KCR8
Bacillus halodurans











721



YfmP


722
Multidrug efflux protein yfmO [Bacillus
UniRef100_O06473
Bacillus subtilis
YfmO



subtilis]


723
YfmM protein [Bacillus subtilis]
UniRef100_O34512
Bacillus subtilis
YfmM


724
YfmL protein [Bacillus subtilis]
UniRef100_O34750
Bacillus subtilis
YfmL


725
YfmJ protein [Bacillus subtilis]
UniRef100_O34812
Bacillus subtilis
YfmJ


726
Hypothetical protein yfmB [Bacillus
UniRef100_O34626
Bacillus subtilis
YfmB



subtilis]


727
General stress protein 17M [Bacillus
UniRef100_P80241
Bacillus subtilis
YflT



subtilis]


728



YflS


729



YflS


730
Putative permease [Clostridium tetani]
UniRef100_Q895A0
Clostridium





tetani


731
Possible Zn-dependent hydrolase,
UniRef100_Q6HKH5
Bacillus
YqgX



beta-lactamase superfamily [Bacillus

thuringiensis



thuringiensis]


732
YflN protein [Bacillus subtilis]
UniRef100_O34409
Bacillus subtilis
YflN


733
Zink-carboxypeptidase [Clostridium
UniRef100_Q898E1
Clostridium



tetani]

tetani


734
Nitric oxide synthase oxygenase
UniRef100_O34453
Bacillus subtilis
YflM



[Bacillus subtilis]


735
Membrane protein, putative [Bacillus
UniRef100_Q72YN4
Bacillus cereus
YvaZ



cereus]


736
Transcriptional regulator, ArsR family
UniRef100_Q632K6
Bacillus cereus ZK



[Bacillus cereus ZK]


737
Putative acylphosphatase [Bacillus
UniRef100_O35031
Bacillus subtilis



subtilis]


738
YflK protein [Bacillus subtilis]
UniRef100_O34542
Bacillus subtilis
YflK


739


740


741
YflG protein [Bacillus subtilis]
UniRef100_O34484
Bacillus subtilis
YflG


742



YflE


743
Hypothetical protein yflB [Bacillus
UniRef100_O34887
Bacillus subtilis



subtilis]


744



YflA


745
Probable PTS system, trehalose-
UniRef100_P39794
Bacillus subtilis
TreP



specific IIBC component [Bacillus



subtilis]


746
Alpha-glucosidase [Bacillus sp.
UniRef100_Q9L872
Bacillus sp.
TreA



DG0303]

DG0303


747
Trehalose operon transcriptional
UniRef100_P39796
Bacillus subtilis
TreR



repressor [Bacillus subtilis]


748
Acetyltransferases
UniRef100_Q8RBZ9
Thermoanaerobacter
YvfD



[Thermoanaerobacter tengcongensis]

tengcongensis


749


750


751
Predicted pyridoxal phosphate-
UniRef100_Q8RBY7
Thermoanaerobacter
SpsC



dependent enzyme apparently involved

tengcongensis



in regulation of cell wall biogenesis



[Thermoanaerobacter tengcongensis]


752



YkuQ










753
UPI000029FB28 UniRef100 entry
UniRef100_UPI000029FB28
YtcB










754
Hypothetical protein MA2181
UniRef100_Q8TNU7
Methanosarcina acetivorans



[Methanosarcina acetivorans]


755











756
Beta 1,4 glucosyltransferase [Bacillus
UniRef100_Q81GJ1
Bacillus cereus
YolJ



cereus]


757
Predicted pyridoxal phosphate-
UniRef100_Q8RC02
Thermoanaerobacter
SpsC



dependent enzyme apparently involved

tengcongensis



in regulation of cell wall biogenesis



[Thermoanaerobacter tengcongensis]


758
Predicted dehydrogenases and related
UniRef100_Q8RC00
Thermoanaerobacter
YrbE



proteins [Thermoanaerobacter

tengcongensis



tengcongensis]


759
UDP-glucose: GDP-mannose
UniRef100_Q8CXB6
Oceanobacillus
TuaD



dehydrogenase [Oceanobacillus

iheyensis



iheyensis]


760
Hypothetical protein [Bacillus cereus
UniRef100_Q635H5
Bacillus cereus
YqkD



ZK]

ZK


761
Hypothetical UPF0087 protein ydeP
UniRef100_P96673
Bacillus subtilis
YdeP



[Bacillus subtilis]


762
Putative NAD(P)H nitroreductase yfkO
UniRef100_O34475
Bacillus subtilis
YfkO



[Bacillus subtilis]


763



YfkN


764
General stress protein 18 [Bacillus
UniRef100_P80876
Bacillus subtilis
YfkM



subtilis]


765
YfkK protein [Bacillus subtilis]
UniRef100_O35019
Bacillus subtilis


766
Amino acid transporter [Bacillus
UniRef100_Q9K5Q5
Bacillus
YflA



halodurans]

halodurans


767
YfkJ protein [Bacillus subtilis]
UniRef100_O35016
Bacillus subtilis
YfkJ


768
Hypothetical protein yfkL precursor
UniRef100_O34418
Bacillus subtilis
YfkL



[Bacillus subtilis]


769
YfkH protein [Bacillus subtilis]
UniRef100_O34437
Bacillus subtilis
YfkH


770
YfkF protein [Bacillus subtilis]
UniRef100_O34929
Bacillus subtilis
YfkF










771
Hypothetical conserved protein
UniRef100_Q8ELS1
Oceanobacillus iheyensis



[Oceanobacillus iheyensis]











772
YfkE protein [Bacillus subtilis]
UniRef100_O34840
Bacillus subtilis
YfkE


773
YfkD protein [Bacillus subtilis]
UniRef100_O34579
Bacillus subtilis
YfkD


774
Thioredoxin-like oxidoreductases
UniRef100_Q81IC7
Bacillus cereus
YfkA



[Bacillus cereus]


775
YfjT protein [Bacillus subtilis]
UniRef100_O35041
Bacillus subtilis


776



YfjS










777
UPI000029390C UniRef100 entry
UniRef100_UPI000029390C
AraM


778











779
YfjQ protein [Bacillus subtilis]
UniRef100_O31543
Bacillus subtilis
YfjQ


780
YfjP protein [Bacillus subtilis]
UniRef100_O31544
Bacillus subtilis
YfjP


781



YfjO


782
YfjM protein [Bacillus subtilis]
UniRef100_O31547
Bacillus subtilis
YfjM


783


784
Hypothetical protein yfjL [Bacillus
UniRef100_P40773
Bacillus subtilis
YfjL



subtilis]










785
UPI00003CB259 UniRef100 entry
UniRef100_UPI00003CB259
YvkB











786
YdhE protein [Bacillus subtilis]
UniRef100_O05496
Bacillus subtilis
YdhE


787
Hypothetical protein yckD precursor
UniRef100_P42402
Bacillus subtilis



[Bacillus subtilis]


788
Hypothetical metabolite transport
UniRef100_O34691
Bacillus subtilis
Ycel



protein ycel [Bacillus subtilis]


789



SacX


790
Levansucrase and sucrase synthesis
UniRef100_P15401
Bacillus subtilis
SacY



operon antiterminator [Bacillus subtilis]


791
Hypothetical protein [Bacillus anthracis]
UniRef100_Q81NL1
Bacillus
YbcF





anthracis


792
Hypothetical protein ybcD [Bacillus
UniRef100_Q639F7
Bacillus cereus
YbcD



cereus ZK]

ZK


793
Potential NADH-quinone
UniRef100_P39755
Bacillus subtilis
NdhF



oxidoreductase subunit 5 [Bacillus



subtilis]


794
YbcI protein [Bacillus subtilis]
UniRef100_O34380
Bacillus subtilis
YbcI


795



YraA


796
TPP-dependent acetoin
UniRef100_Q81PM6
Bacillus
AcoA



dehydrogenase E1 alpha-subunit

anthracis



[Bacillus anthracis]


797
TPP-dependent acetoin
UniRef100_Q736U7
Bacillus cereus
AcoB



dehydrogenase E1 beta-subunit



[Bacillus cereus]


798
Dihydrolipoyllysine-residue
UniRef100_O31550
Bacillus subtilis
AcoC



acetyltransferase component of acetoin



cleaving system [Bacillus subtilis]


799
Dihydrolipoyl dehydrogenase [Bacillus
UniRef100_O34324
Bacillus subtilis
AcoL



subtilis]


800
Acetoin operon transcriptional
UniRef100_Q736V6
Bacillus cereus
AcoR



activator, putative [Bacillus cereus]


801
Hypothetical UPF0060 protein yfjF
UniRef100_O31553
Bacillus subtilis
YfjF



[Bacillus subtilis]


802
Maltose-6′-phosphate glucosidase
UniRef100_P54716
Bacillus subtilis
MalA



[Bacillus subtilis]


803
HTH-type transcriptional regulator glvR
UniRef100_P54717
Bacillus subtilis
YfiA



[Bacillus subtilis]


804
PTS system, arbutin-like IIBC
UniRef100_P54715
Bacillus subtilis
MalP



component [Bacillus subtilis]










805
UPI00003651CF UniRef100 entry
UniRef100_UPI00003651CF



806
UPI000034AA1D UniRef100 entry
UniRef100_UPI000034AA1D











807
Transcriptional regulator, MarR family
UniRef100_Q638I2
Bacillus cereus
YvaP



[Bacillus cereus ZK]

ZK


808



YfiD


809
Hypothetical protein yfiE [Bacillus
UniRef100_P54721
Bacillus subtilis
YfiE



subtilis]


810
Xylosidase/arabinosidase [Bacteroides
UniRef100_Q8A036
Bacteroides
XynB



thetaiotaomicron]

thetaiotaomicron


811
Xylan beta-1,4-xylosidase [Bacillus
UniRef100_Q9K6P5
Bacillus
XynB



halodurans]

halodurans


812
Trancriptional regulator of AraC family
UniRef100_Q97FW8
Clostridium
YbfI



[Clostridium acetobutylicum]

acetobutylicum


813



YtcQ










814
NAD(P)H dehydrogenase, quinone
UniRef100_Q638S8
Bacillus cereus ZK



family [Bacilius cereus ZK]











815
Mutator MutT protein [Bacillus
UniRef100_Q9K8B7
Bacillus
YjhB



halodurans]

halodurans


816
YfiT protein [Bacillus subtilis]
UniRef100_O31562
Bacillus subtilis
YfiT


817
YfiX [Bacillus subtilis]
UniRef100_O52961
Bacillus subtilis
YfiX


818
Hypothetical protein yfhB [Bacillus
UniRef100_O31570
Bacillus subtilis
YfhB



subtilis]


819
YfhC protein [Bacillus subtilis]
UniRef100_O31571
Bacillus subtilis
YfhC


820
Hypothetical protein yfhD [Bacillus
UniRef100_O31572
Bacillus subtilis



subtilis]


821


822
BH0923 homolog [Bacillus cereus]
UniRef100_Q81IA0
Bacillus cereus


823
Hypothetical UPF0105 protein yfhF
UniRef100_O31574
Bacillus subtilis
YfhF



[Bacillus subtilis]


824
Regulatory protein recX [Bacillus
UniRef100_O31575
Bacillus subtilis
YfhG



subtilis]


825
YfhH protein [Bacillus subtilis]
UniRef100_O31576
Bacillus subtilis
YfhH


826


827
YfhJ protein [Bacillus subtilis]
UniRef100_O31578
Bacillus subtilis


828
CsbB protein [Bacillus subtilis]
UniRef100_Q45539
Bacillus subtilis
CsbB










829
Hypothetical protein SE1997
UniRef100_Q8CR87
Staphylococcus epidermidis



[Staphylococcus epidermidis]











830
YfhO protein [Bacillus subtilis]
UniRef100_O31582
Bacillus subtilis
YfhO


831
YfhP protein [Bacillus subtilis]
UniRef100_O31583
Bacillus subtilis
YfhP


832
YfhQ protein [Bacillus subtilis]
UniRef100_O31584
Bacillus subtilis
YfhQ


833
YfhS protein [Bacillus subtilis]
UniRef100_O31585
Bacillus subtilis


834
Unidentfied dehydrogenase [Bacillus
UniRef100_P71079
Bacillus subtilis
FabL



subtilis]


835


836
Hypothetical protein ygaB [Bacillus
UniRef100_P71080
Bacillus subtilis



subtilis]


837
YgaC protein [Bacillus subtilis]
UniRef100_Q796Z1
Bacillus subtilis
YgaC


838
Unidentified transporter-ATP binding
UniRef100_P71082
Bacillus subtilis
YgaD



[Bacillus subtilis]


839
Oligopeptide ABC transporter [Bacillus
UniRef100_Q9K6T0
Bacillus
AppD



halodurans]

halodurans


840
Oligopeptide ABC transporter [Bacillus
UniRef100_Q9K6T1
Bacillus
AppF



halodurans]

halodurans


841
Dipeptide transporter protein DppA
UniRef100_P94310
Bacillus firmus
OppA



[Bacillus firmus]


842
Dipeptide ABC transporter [Bacillus
UniRef100_Q9K6T3
Bacillus
AppB



halodurans]

halodurans


843
Dipeptide transport system permease
UniRef100_P94312
Bacillus
AppC



protein dppC [Bacillus pseudofirmus]

pseudofirmus


844
Hypothetical 40.7 kd protein [Bacillus
UniRef100_P71083
Bacillus subtilis
YgaE



subtilis]


845
Glutamate-1-semialdehyde 2,1-
UniRef100_P71084
Bacillus subtilis
GsaB



aminomutase 2 [Bacillus subtilis]


846
YgaF protein [Bacillus subtilis]
UniRef100_Q796Y8
Bacillus subtilis
YgaF


847
Peroxide operon regulator [Bacillus
UniRef100_P71086
Bacillus subtilis
PerR



subtilis]


848


849
Hypothetical protein ygxA [Bacillus
UniRef100_Q04385
Bacillus subtilis
YgxA



subtilis]


850



RapD


851


852


853


854


855










856
Hypothetical protein [Bacillus
UniRef100_Q6HH72
Bacillus thuringiensis



thuringiensis]











857



YxiD










858
Hypothetical protein [uncultured
UniRef100_Q64DF2
uncultured archaeon



archaeon GZfos18F2]

GZfos18F2


859









860

YxiD


861

RapE










862
Putative membrane protein
UniRef100_Q82NM0
Streptomyces avermitilis



[Streptomyces avermitilis]











863
3-dehydroquinate dehydratase [Listeria
UniRef100_Q8Y9N4
Listeria
AroC



monocytogenes]

monocytogenes


864



ThiC


865
Putative aliphatic sulfonates transport
UniRef100_P97027
Bacillus subtilis
SsuB



ATP-binding protein ssuB [Bacillus



subtilis]


866
Putative aliphatic sulfonates binding
UniRef100_P40400
Bacillus subtilis
SsuA



protein precursor [Bacillus subtilis]


867
Putative aliphatic sulfonates transport
UniRef100_P40401
Bacillus subtilis
SsuC



permease protein ssuC [Bacillus



subtilis]


868



SsuD


869



SsuD


870
Hypothetical lipoprotein ygaO
UniRef100_P97029
Bacillus subtilis
YgaO



precursor [Bacillus subtilis]










871
DNA-binding protein [Bacillus
UniRef100_Q81V18
Bacillus anthracis



anthracis]


872











873
ABC-type multidrug transport system,
UniRef100_Q8RBH0
Thermoanaerobacter
YhaQ



ATPase component

tengcongensis



[Thermoanaerobacter tengcongensis]


874


875


876


877


878
30S ribosomal protein S14-2 [Bacillus
UniRef100_O31587
Bacillus subtilis



subtilis]


879
Hypothetical protein yhzB [Bacillus
UniRef100_O31588
Bacillus subtilis
YhzB



subtilis]


880
Hypothetical 48.5 kd protein [Bacillus
UniRef100_P97030
Bacillus subtilis
YhbA



subtilis]


881
Hypothetical 35.8 kd protein [Bacillus
UniRef100_P97031
Bacillus subtilis
YhbB



subtilis]


882
CspR [Bacillus subtilis]
UniRef100_Q45512
Bacillus subtilis
CspR


883
Hypothetical 27 kd protein [Bacillus
UniRef100_P97032
Bacillus subtilis
YhbD



subtilis]


884
Hypothetical Cytosolic Protein [Bacillus
UniRef100_Q813H1
Bacillus cereus
YhbE



cereus]


885
Hypothetical protein [Bacillus anthracis]
UniRef100_Q81PD2
Bacillus
YhbF





anthracis


886


887
PrkA protein [Bacillus subtilis]
UniRef100_P39134
Bacillus subtilis
PrkA


888
Stress response UPF0229 protein
UniRef100_P45742
Bacillus subtilis
YhbH



yhbH [Bacillus subtilis]


889
YhbJ protein [Bacillus subtilis]
UniRef100_O31593
Bacillus subtilis
YhbJ


890
Hypothetical transport protein yhcA
UniRef100_P54585
Bacillus subtilis
YhcA



[Bacillus subtilis]


891
Hypothetical protein yhcB [Bacillus
UniRef100_P54586
Bacillus subtilis
YhcB



subtilis]


892



YhcC


893


894
Hypothetical ABC transporter ATP-
UniRef100_P54591
Bacillus subtilis



binding protein yhcG [Bacillus subtilis]


895
Hypothetical ABC transporter ATP-
UniRef100_P54592
Bacillus subtilis
YhcH



binding protein yhcH [Bacillus subtilis]


896
Hypothetical protein yhcI [Bacillus
UniRef100_P54593
Bacillus subtilis
YhcI



subtilis]


897
Cold shock protein cspB [Bacillus
UniRef100_P32081
Bacillus subtilis



subtilis]


898
ABC transporter, ATP-binding protein
UniRef100_Q6HP89
Bacillus
YusC



[Bacillus thuringiensis]

thuringiensis


899
MW0417 protein [Staphylococcus
UniRef100_Q8NY20
Staphylococcus
YusB



aureus]

aureus


900
ABC transporter substrate-binding
UniRef100_Q81IN6
Bacillus cereus
YhcJ



protein [Bacillus cereus]


901
Hypothetical symporter yhcL [Bacillus
UniRef100_P54596
Bacillus subtilis
YhcL



subtilis]


902
Hypothetical protein yhcM [Bacillus
UniRef100_P54597
Bacillus subtilis
YhcM



subtilis]


903
Acylamino-acid-releasing enzyme
UniRef100_Q8CXN6
Oceanobacillus
YuxL



[Oceanobacillus iheyensis]

iheyensis


904
Lipoprotein yhcN precursor [Bacillus
UniRef100_P54598
Bacillus subtilis
YhcN



subtilis]


905
Hypothetical protein yhcP [Bacillus
UniRef100_P54600
Bacillus subtilis
YhcP



subtilis]


906
Hypothetical protein yhcQ [Bacillus
UniRef100_P54601
Bacillus subtilis
YhcQ



subtilis]


907
Two-component sensor histidine
UniRef100_O31661
Bacillus subtilis
KinE



kinase [Bacillus subtilis]


908
Hypothetical protein yhcR precursor
UniRef100_P54602
Bacillus subtilis
YhcR



[Bacillus subtilis]


909
Hypothetical protein yhcS [Bacillus
UniRef100_P54603
Bacillus subtilis
YhcS



subtilis]


910
Hypothetical pseudouridine synthase
UniRef100_P54604
Bacillus subtilis
YhcT



yhcT [Bacillus subtilis]


911
Hypothetical protein yhcU [Bacillus
UniRef100_P54605
Bacillus subtilis
YhcU



subtilis]


912
Hypothetical protein yhcV [Bacillus
UniRef100_P54606
Bacillus subtilis
YhcV



subtilis]


913
Hypothetical protein yhcW [Bacillus
UniRef100_P54607
Bacillus subtilis
YhcW



subtilis]


914
Hypothetical UPF0012 protein yhcX
UniRef100_P54608
Bacillus subtilis
YhcX



[Bacillus subtilis]


915
ABC transporter [Bacillus halodurans]
UniRef100_Q9KBA3
Bacillus
YdiF





halodurans


916
Hypothetical transport protein yrhG
UniRef100_O05399
Bacillus subtilis
YrhG



[Bacillus subtilis]


917
Lin0826 protein [Listeria innocua]
UniRef100_Q92DI7
Listeria innocua
YwkB


918
Alcohol dehydrogenase [Bacillus
UniRef100_Q81CT4
Bacillus cereus
YogA



cereus]


919
Glycerol uptake operon antiterminator
UniRef100_P30300
Bacillus subtilis
GlpP



regulatory protein [Bacillus subtilis]


920
Glycerol uptake facilitator protein
UniRef100_P18156
Bacillus subtilis
GlpF



[Bacillus subtilis]


921
Glycerol kinase [Bacillus subtilis]
UniRef100_P18157
Bacillus subtilis
GlpK


922
Aerobic glycerol-3-phosphate
UniRef100_P18158
Bacillus subtilis
GlpD



dehydrogenase [Bacillus subtilis]


923
Alpha-phosphoglucomutase [Bacillus
UniRef100_Q68VA2
Bacillus subtilis
YhxB



subtilis subsp. subtilis]

subsp. subtilis


924
Hypothetical conserved protein
UniRef100_Q8ELS7
Oceanobacillus
YcgB



[Oceanobacillus iheyensis]

iheyensis










925
Transcriptional regulator
UniRef100_Q8ELS8
Oceanobacillus iheyensis



[Oceanobacillus iheyensis]











926



YhcY


927
Hypothetical protein yhcZ [Bacillus
UniRef100_O07528
Bacillus subtilis
YhcZ



subtilis]


928
Hypothetical protein yhdA [Bacillus
UniRef100_O07529
Bacillus subtilis
YhdA



subtilis]


929


930


931
Hypothetical UPF0074 protein yhdE
UniRef100_O07573
Bacillus subtilis
YhdE



[Bacillus subtilis]


932
Flavohemoprotein [Bacillus halodurans]
UniRef100_Q9RC40
Bacillus
Hmp





halodurans


933
Stage V sporulation protein R [Bacillus
UniRef100_P37875
Bacillus subtilis
SpoVR



subtilis]


934
Probable endopeptidase lytE precursor
UniRef100_P54421
Bacillus subtilis
LytE



[Bacillus subtilis]


935



CitR


936



CitA










937
Glucose dehydrogenase-B [Bacillus
UniRef100_Q9Z9R3
Bacillus halodurans



halodurans]











938
Hypothetical oxidoreductase yhdF
UniRef100_O07575
Bacillus subtilis
YhdF



[Bacillus subtilis]


939
Hypothetical protein yhdH [Bacillus
UniRef100_O07577
Bacillus subtilis
YhdH



subtilis]










940
2,4-diaminobutyrate decarboxylase
UniRef100_Q9KFB9
Bacillus halodurans



[Bacillus halodurans]











941
YdeE protein [Bacillus subtilis]
UniRef100_P96662
Bacillus subtilis
YdeE


942
YdeL protein [Bacillus subtilis]
UniRef100_P96669
Bacillus subtilis
YdeL


943
BH1582 protein [Bacillus halodurans]
UniRef100_Q9KCI9
Bacillus
YhdJ





halodurans










944
YhdK [Bacillus subtilis subsp.
UniRef100_Q7X2K9
Bacillus subtilis subsp.



spizizenii]

spizizenii











945
YhdL [Bacillus subtilis subsp. spizizenii]
UniRef100_Q7X2L0
Bacillus subtilis
YhdL





subsp. spizizenii


946
Hypothetical protein yhdM (RNA
UniRef100_O07582
Bacillus subtilis
SigM



polymerase ECF (Extracytoplasmic



function)-type sigma factor) [Bacillus



subtilis]


947



YrkC


948
Hypothetical protein yhdO [Bacillus
UniRef100_O07584
Bacillus subtilis
YhdO



subtilis]


949










950
Acyl-CoA thioesterase 1 [Clostridium
UniRef100_Q97DR5
Clostridium acetobutylicum



acetobutylicum]










951
UPI00003CB259 UniRef100 entry
UniRef100_UPI00003CB259
YvkB











952



YhdP


953
HTH-type transcriptional regulator
UniRef100_O07586
Bacillus subtilis
YhdQ



cueR [Bacillus subtilis]


954



YhdT


955










956
BH3511 protein [Bacillus halodurans]
UniRef100_Q9K762
Bacillus halodurans











957
Sporulation specific N-acetylmuramoyl-
UniRef100_Q8CX69
Oceanobacillus
CwlC



L-alanine amidase [Oceanobacillus

iheyensis



iheyensis]


958
Protein crcB homolog 1 [Bacillus
UniRef100_O07590
Bacillus subtilis
YhdU



subtilis]


959
Protein crcB homolog 2 [Bacillus
UniRef100_O07591
Bacillus subtilis
YhdV



subtilis]


960



YhdW


961


962
Hypothetical UPF0003 protein yhdY
UniRef100_O07594
Bacillus subtilis
YhdY



[Bacillus subtilis]


963
NAD-dependent deacetylase [Bacillus
UniRef100_O07595
Bacillus subtilis
YhdZ



subtilis]


964


965
Hypothetical protein yheN [Bacillus
UniRef100_O07596
Bacillus subtilis
YheN



subtilis]


966



Dat


967
Na(+)/H(+) antiporter [Bacillus subtilis]
UniRef100_O07553
Bacillus subtilis
NhaC


968
Hypothetical protein yoxA [Bacillus
UniRef100_P39840
Bacillus subtilis
YoxA



subtilis]


969
Hypothetical protein ydhH [Bacillus
UniRef100_O05500
Bacillus subtilis
YdhH



subtilis]










970
Hypothetical protein [Bacillus cereus
UniRef100_Q63EB4
Bacillus cereus ZK



ZK]











971
Hypothetical protein [Bacillus cereus]
UniRef100_Q81GF4
Bacillus cereus



972
Stress response protein nhaX [Bacillus
UniRef100_O07552
Bacillus subtilis
NhaX



subtilis]


973
Hypothetical protein yhel [Bacillus
UniRef100_O07550
Bacillus subtilis
YheI



subtilis]


974
Hypothetical protein yheH [Bacillus
UniRef100_O07549
Bacillus subtilis
YheH



subtilis]


975
Hypothetical protein yheG [Bacillus
UniRef100_O07548
Bacillus subtilis
YheG



subtilis]


976
Small, acid-soluble spore protein B
UniRef100_P04832
Bacillus subtilis



[Bacillus subtilis]










977
BH1139 protein [Bacillus halodurans]
UniRef100_Q9KDS2
Bacillus halodurans











978
Sugar ABC transporter ATP-binding
UniRef100_Q8CUH3
Oceanobacillus
MsmX



protein [Oceanobacillus iheyensis]

iheyensis


979
Fis-type helix-turn-helix domain protein
UniRef100_Q73A84
Bacillus cereus
YxkF



[Bacillus cereus]


980
Hypothetical protein yheE [Bacillus
UniRef100_O07546
Bacillus subtilis



subtilis]


981
Hypothetical protein yheD [Bacillus
UniRef100_O07545
Bacillus subtilis
YheD



subtilis]


982
Hypothetical protein yheC [Bacillus
UniRef100_O07544
Bacillus subtilis
YheC



subtilis]


983
Hypothetical protein yheB [Bacillus
UniRef100_O07543
Bacillus subtilis
YheB



subtilis]


984
Hypothetical protein yheA [Bacillus
UniRef100_O07542
Bacillus subtilis
YheA



subtilis]


985
Stress response protein yhaX [Bacillus
UniRef100_O07539
Bacillus subtilis
YhaX



subtilis]


986



HemZ


987
YhaR protein [Bacillus subtilis]
UniRef100_O07533
Bacillus subtilis
YhaR


988
Response regulator aspartate
UniRef100_P96649
Bacillus subtilis
RapI



phosphatase I [Bacillus subtilis]


989


990
Hypothetical protein yhaQ [Bacillus
UniRef100_O07524
Bacillus subtilis
YhaQ



subtilis]


991
Hypothetical protein yhaP [Bacillus
UniRef100_O07523
Bacillus subtilis
YhaP



subtilis]


992



YhaO


993
Hypothetical protein yhaN [Bacillus
UniRef100_O08455
Bacillus subtilis
YhaN



subtilis]


994



YhaM


995
Hypothetical protein yhaL [Bacillus
UniRef100_O07520
Bacillus subtilis



subtilis]


996
Foldase protein prsA precursor
UniRef100_P24327
Bacillus subtilis
PrsA



[Bacillus subtilis]


997


998
Hypothetical protein yhaK [Bacillus
UniRef100_O07519
Bacillus subtilis



subtilis]


999
Hypothetical protein yhaI [Bacillus
UniRef100_O07517
Bacillus subtilis
YhaI



subtilis]


1000
Protease production regulatory protein
UniRef100_P11065
Bacillus subtilis
Hpr



hpr [Bacillus subtilis]


1001
Hypothetical protein yhaH [Bacillus
UniRef100_O07516
Bacillus subtilis
YhaH



subtilis]


1002
Probable tryptophan transport protein
UniRef100_O07515
Bacillus subtilis
YhaG



[Bacillus subtilis]


1003
Phosphoserine aminotransferase
UniRef100_P80862
Bacillus subtilis
SerC



[Bacillus subtilis]


1004
Hit protein [Bacillus subtilis]
UniRef100_O07513
Bacillus subtilis
Hit


1005


1006
ABC-type transporter ATP-binding
UniRef100_P55339
Bacillus subtilis
EcsA



protein ecsA [Bacillus subtilis]


1007
Protein ecsB [Bacillus subtilis]
UniRef100_P55340
Bacillus subtilis
EcsB


1008
Protein ecsC [Bacillus subtilis]
UniRef100_P55341
Bacillus subtilis
EcsC


1009
YhaA protein [Bacillus subtilis]
UniRef100_O07598
Bacillus subtilis
YhaA


1010
Hypothetical protein yhfA [Bacillus
UniRef100_O07599
Bacillus subtilis
YhfA



subtilis]


1011
Hypothetical protein yhgC [Bacillus
UniRef100_P38049
Bacillus subtilis
YhgC



subtilis]


1012
Penicillin-binding protein 1F [Bacillus
UniRef100_P38050
Bacillus subtilis
PbpF



subtilis]


1013
Uroporphyrinogen decarboxylase
UniRef100_P32395
Bacillus subtilis
HemE



[Bacillus subtilis]


1014
Ferrochelatase [Bacillus subtilis]
UniRef100_P32396
Bacillus subtilis
HemH


1015
Protoporphyrinogen oxidase [Bacillus
UniRef100_P32397
Bacillus subtilis
HemY



subtilis]


1016



YhgD


1017
Hypothetical protein yhgE [Bacillus
UniRef100_P32399
Bacillus subtilis
YhgE



subtilis]


1018
3-oxoacyl-[acyl-carrier-protein]
UniRef100_O07600
acyl-carrier-
FabHB



synthase III protein 2 [Bacillus subtilis]

protein


1019
Hypothetical protein yhfE [Bacillus
UniRef100_O07603
Bacillus subtilis
YhfE



subtilis]


1020


1021
Hypothetical protein yhfG [Bacillus
UniRef100_O07605
Bacillus subtilis
GltT



subtilis]


1022
Hypothetical protein yhfI [Bacillus
UniRef100_O07607
Bacillus subtilis
YhfI



subtilis]


1023
Hypothetical protein yhfJ [Bacillus
UniRef100_O07608
Bacillus subtilis
YhfJ



subtilis]


1024
Hypothetical protein yhfL [Bacillus
UniRef100_O07610
Bacillus subtilis
YhfL



subtilis]


1025
Hypothetical protein yhfM precursor
UniRef100_O07611
Bacillus subtilis
YhfM



[Bacillus subtilis]










1026
BH2909 protein [Bacillus halodurans]
UniRef100_Q9K8U3
Bacillus halodurans











1027
Branched-chain amino acid transporter
UniRef100_Q9K8U2
Bacillus
AzlC



[Bacillus halodurans]

halodurans










1028
BH2911 protein [Bacillus halodurans]
UniRef100_Q9K8U1
Bacillus halodurans











1029
Putative metalloprotease yhfN [Bacillus
UniRef100_P40769
Bacillus subtilis
YhfN



subtilis]


1030



AprE


1031
Transporter, drug/metabolite exporter
UniRef100_Q63D40
Bacillus cereus
YdeD



family [Bacillus cereus ZK]

ZK


1032
Hypothetical protein yhfQ [Bacillus
UniRef100_O07616
Bacillus subtilis
YhfQ



subtilis]


1033
YfmD protein [Bacillus subtilis]
UniRef100_O34933
Bacillus subtilis
YfmD


1034
YfmE protein [Bacillus subtilis]
UniRef100_O34832
Bacillus subtilis
YfmE


1035
Hypothetical protein yhfR [Bacillus
UniRef100_O07617
Bacillus subtilis
YhfR



subtilis]


1036
Heme-based aerotactic transducer
UniRef100_O07621
Bacillus subtilis
HemAT



hemAT [Bacillus subtilis]


1037
Rieske 2Fe-2S iron-sulfur protein,
UniRef100_Q73E94
Bacillus cereus
YhfW



putative [Bacillus cereus]


1038



YhxC


1039
Hypothetical protein yhzC [Bacillus
UniRef100_O31594
Bacillus subtilis



subtilis]


1040



ComK


1041



YhjD


1042



YhjE


1043
Signal peptidase I V [Bacillus subtilis]
UniRef100_O07560
Bacillus subtilis
SipV


1044
Minor extracellular protease epr
UniRef100_P16396
Bacillus subtilis
Epr



precursor [Bacillus subtilis]


1045
Putative permease [Klebsiella
UniRef100_Q765R6
Klebsiella
YybO



pneumoniae]

pneumoniae


1046
Hypothetical protein [Enterococcus
UniRef100_Q82ZQ4
Enterococcus
PucR



faecalis]

faecalis


1047
Putative allantoinase [Staphylococcus
UniRef100_Q9EV52
Staphylococcus
PucH



xylosus]

xylosus


1048
Peptidase, M20/M25/M40 family
UniRef100_Q82ZQ2
Enterococcus
YurH



[Enterococcus faecalis]

faecalis


1049
Hypothetical protein STY0574
UniRef100_Q8XFX7
Salmonella typhi



[Salmonella typhi]


1050
Ureidoglycolate dehydrogenase
UniRef100_Q838P9
Enterococcus
YjmC



[Enterococcus faecalis]

faecalis


1051
Ureidoglycolate dehydrogenase
UniRef100_Q838P9
Enterococcus
YjmC



[Enterococcus faecalis]

faecalis


1052
Hypothetical protein [Enterococcus
UniRef100_Q838Q3
Enterococcus
SucD



faecalis]

faecalis










1053
Hypothetical protein [Enterococcus
UniRef100_Q838Q3
Enterococcus faecalis



faecalis]











1054






1055
Carbamate kinase [Clostridium tetani]
UniRef100_Q890W1
Clostridium





tetani


1056
Major facilitator family transporter
UniRef100_Q838Q1
Enterococcus
YcbE



[Enterococcus faecalis]

faecalis


1057
Hypothetical protein yjfA precursor
UniRef100_O34554
Bacillus subtilis



[Bacillus subtilis]


1058
Response regulator aspartate
UniRef100_O32294
Bacillus subtilis
RapG



phosphatase G [Bacillus subtilis]


1059


1060


1061


1062
Lacl-family transcription regulator
UniRef100_O34829
Bacillus subtilis
MsmR



[Bacillus subtilis]


1063
Multiple sugar-binding protein [Bacillus
UniRef100_O34335
Bacillus subtilis
MsmE



subtilis]


1064
Sugar transporter [Bacillus subtilis]
UniRef100_O34706
Bacillus subtilis
AmyD


1065
Sugar transporter [Bacillus subtilis]
UniRef100_O34518
Bacillus subtilis
AmyC


1066
Alpha-galactosidase [Bacillus subtilis]
UniRef100_O34645
Bacillus subtilis
MelA


1067
Hypothetical protein yhjN [Bacillus
UniRef100_O07568
Bacillus subtilis
YhjN



subtilis]










1068
Spore coat-associated protein JA
UniRef100_Q63FK5
Bacillus cereus ZK



[Bacillus cereus ZK]











1069
CotJB protein [Bacillus subtilis]
UniRef100_Q45537
Bacillus subtilis



1070
CotJC protein [Bacillus subtilis]
UniRef100_Q45538
Bacillus subtilis
CotJC


1071
Long-chain fatty-acid-CoA ligase
UniRef100_Q9KDT0
Bacillus
YngI



[Bacillus halodurans]

halodurans


1072
Hypothetical protein yhjO [Bacillus
UniRef100_O07569
Bacillus subtilis
YhjO



subtilis]


1073
Hypothetical protein [Bacillus cereus
UniRef100_Q63FZ3
Bacillus cereus
LytB



ZK]

ZK


1074
Sensor histidine kinase [Bacillus
UniRef100_Q6HNG3
Bacillus
PhoR



thuringiensis]

thuringiensis


1075
Two-component response regulator
UniRef100_Q81I36
Bacillus cereus
YclJ



[Bacillus cereus]


1076



YhjR


1077
Putative molybdate binding protein,
UniRef100_O32208
Bacillus subtilis
YvgL



YvgL [Bacillus subtilis]


1078
Putative molybdate transport protein,
UniRef100_O32209
Bacillus subtilis
YvgM



YvgM [Bacillus subtilis]


1079
ATP-dependent nuclease subunit B
UniRef100_P23477
Bacillus subtilis
AddB



[Bacillus subtilis]


1080
ATP-dependent nuclease subunit A
UniRef100_P23478
Bacillus subtilis
AddA



[Bacillus subtilis]


1081
Exonuclease sbcD homolog [Bacillus
UniRef100_P23479
Bacillus subtilis
SbcD



subtilis]


1082
Nuclease sbcCD subunit C [Bacillus
UniRef100_O06714
Bacillus subtilis
YirY



subtilis]


1083
Probable spore germination protein
UniRef100_O06716
Bacillus subtilis



gerPF [Bacillus subtilis]


1084
Probable spore germination protein
UniRef100_O06717
Bacillus subtilis
GerPE



gerPE [Bacillus subtilis]


1085
Probable spore germination protein
UniRef100_O06718
Bacillus subtilis



gerPD [Bacillus subtilis]


1086
Probable spore germination protein
UniRef100_O06719
Bacillus subtilis
GerPC



gerPC [Bacillus subtilis]


1087
Probable spore germination protein
UniRef100_O06720
Bacillus subtilis



gerPB [Bacillus subtilis]


1088
Probable spore germination protein
UniRef100_O06721
Bacillus subtilis



gerPA [Bacillus subtilis]


1089
Hypothetical protein yitR [Bacillus
UniRef100_O06753
Bacillus subtilis



subtilis]


1090


1091
Spore coat protein H [Bacillus cereus]
UniRef100_Q81EE9
Bacillus cereus
CotH


1092



CotG


1093
YisK [Bacillus subtilis]
UniRef100_O06724
Bacillus subtilis
YisK


1094
Hypothetical protein yloQ [Bacillus
UniRef100_Q63CF2
Bacillus cereus
YloQ



cereus ZK]

ZK


1095
YisL [Bacillus subtilis]
UniRef100_O06725
Bacillus subtilis
YisL


1096
Hypothetical protein yisN [Bacillus
UniRef100_O06727
Bacillus subtilis
YisN



subtilis]


1097
Asparagine synthetase [glutamine-
UniRef100_O05272
glutamine-
AsnO



hydrolyzing] 3 [Bacillus subtilis]

hydrolyzing


1098



NrgA










1099
Nitrogen regulatory PII protein
UniRef100_Q8ERT8
Oceanobacillus iheyensis



[Oceanobacillus iheyensis]











1100
YisQ [Bacillus subtilis]
UniRef100_O07940
Bacillus subtilis
YisQ


1101
Putative HTH-type transcriptional
UniRef100_P40331
Bacillus subtilis
YisR



regulator yisR [Bacillus subtilis]


1102
Acetyltransferase, GNAT family
UniRef100_Q63C80
Bacillus cereus
YokL



[Bacillus cereus ZK]

ZK


1103
HTH-type transcriptional regulator
UniRef100_P37947
Bacillus subtilis
DegA



degA [Bacillus subtilis]


1104
Hypothetical oxidoreductase yisS
UniRef100_P40332
Bacillus subtilis
YisS



[Bacillus subtilis]


1105
YisV protein [Bacillus subtilis]
UniRef100_Q796Q6
Bacillus subtilis
YisV


1106
Diaminobutyrate-pyruvate
UniRef100_Q9K9M1
Bacillus
GabT



transaminase [Bacillus halodurans]

halodurans


1107
L-2,4-diaminobutyrate decarboxylase
UniRef100_Q8YZR2
Anabaena sp.



[Anabaena sp.]


1108
AII0394 protein [Anabaena sp.]
UniRef100_Q8YZR3
Anabaena sp.










1109
BH2621 protein [Bacillus halodurans]
UniRef100_Q9K9M4
Bacillus halodurans


1110
BH2620 protein [Bacillus halodurans]
UniRef100_Q9K9M5
Bacillus halodurans


1111
BH2618 protein [Bacillus halodurans]
UniRef100_Q9K9M7
Bacillus halodurans











1112
YitI protein [Bacillus subtilis]
UniRef100_O06744
Bacillus subtilis
YitI


1113
Glr2355 protein [Gloeobacter
UniRef100_Q7N129
Gloeobacter
YcdF



violaceus]

violaceus


1114
BH0411 protein [Bacillus halodurans]
UniRef100_Q9KFR6
Bacillus
YobV





halodurans


1115
5-methyltetrahydrofolate S-
UniRef100_Q9KCE1
Bacillus
YitJ



homocysteine methyltransferase

halodurans



[Bacillus halodurans]


1116
YitJ [Bacillus subtilis]
UniRef100_O06745
Bacillus subtilis
YitJ


1117
Hypothetical UPF0234 protein yitk
UniRef100_O06746
Bacillus subtilis
YitK



[Bacillus subtilis]


1118
YitL protein [Bacillus subtilis]
UniRef100_O06747
Bacillus subtilis
YitL


1119


1120


1121
Hypothetical UPF0230 protein yitS
UniRef100_P70945
Bacillus subtilis
YitS



[Bacillus subtilis]


1122
Hypothetical protein yitT [Bacillus
UniRef100_P39803
Bacillus subtilis
YitT



subtilis]


1123
Intracellular proteinase inhibitor
UniRef100_P39804
Bacillus subtilis
Ipi



[Bacillus subtilis]


1124
GMP reductase [Bacillus subtilis]
UniRef100_O05269
Bacillus subtilis
GuaC


1125


1126


1127
Putative orf protein [Bacillus subtilis]
UniRef100_P70947
Bacillus subtilis
YitU


1128
Putative orf protein [Bacillus subtilis]
UniRef100_P70948
Bacillus subtilis
YitV


1129



YitW


1130
N-acetyl-gamma-glutamyl-phosphate
UniRef100_P23715
Bacillus subtilis
ArgC



reductase [Bacillus subtilis]


1131
Arginine biosynthesis bifunctional
UniRef100_Q9ZJ14
Includes:
ArgJ



protein argJ [Includes: Glutamate N-

Glutamate N-



acetyltransferase (EC 2.3.1.35)

acetyltransferase



(Ornithine acetyltransferase) (Ornithine

(EC 2.3.1.35)



transacetylase) (OATase); Amino-acid

(Ornithine



acetyltransferase (EC 2.3.1.1) (N-

acetyltransferase)



acetylglutamate synthase) (AGS)]

(Ornithine



[Contai

transacetylase)





(OATase);





Amino-acid





acetyltransferase





(EC 2.3.1.1) (N-





acetylglutamate





synthase) (AGS)


1132
Acetylglutamate kinase [Bacillus
UniRef100_P36840
Bacillus subtilis
ArgB



subtilis]


1133
Acetylornithine aminotransferase
UniRef100_P36839
Bacillus subtilis
ArgD



[Bacillus subtilis]


1134
Carbamoyl-phosphate synthase,
UniRef100_P36838
Bacillus subtilis
CarA



arginine-specific, small chain [Bacillus



subtilis]


1135
Carbamoyl-phosphate synthase,
UniRef100_P18185
Bacillus subtilis
CarB



arginine-specific, large chain [Bacillus



subtilis]


1136
Ornithine carbamoyltransferase
UniRef100_P18186
Bacillus subtilis
ArgF



[Bacillus subtilis]


1137
Undecaprenyl-diphosphatase 1
UniRef100_Q81HV4
Bacillus cereus
YubB



[Bacillus cereus]


1138
YjzC protein [Bacillus subtilis]
UniRef100_O34585
Bacillus subtilis


1139


1140
Hypothetical protein yjaU [Bacillus
UniRef100_O35001
Bacillus subtilis
YjaU



subtilis]


1141
ArgF and med genes, partial and
UniRef100_O32435
Bacillus subtilis
YjaV



complete cds [Bacillus subtilis]


1142
Transcriptional activator protein med
UniRef100_O32436
Bacillus subtilis
Med



precursor [Bacillus subtilis]


1143
ComG operon repressor [Bacillus
UniRef100_O32437
Bacillus subtilis



subtilis]


1144
Hypothetical protein yjzB [Bacillus
UniRef100_O34891
Bacillus subtilis



subtilis]


1145
3-oxoacyl-[acyl-carrier-protein]
UniRef100_O34746
acyl-carrier-
FabHA



synthase III protein 1 [Bacillus subtilis]

protein


1146
Beta-ketoacyl-acyl carrier protein
UniRef100_O34340
Bacillus subtilis
FabF



synthase II [Bacillus subtilis]


1147
YjaZ protein [Bacillus subtilis]
UniRef100_O31596
Bacillus subtilis
YjaZ


1148
Oligopeptide transport ATP-binding
UniRef100_P42064
Bacillus subtilis
AppD



protein appD [Bacillus subtilis]


1149
Oligopeptide transport ATP-binding
UniRef100_P42065
Bacillus subtilis
AppF



protein appF [Bacillus subtilis]


1150
Oligopeptide-binding protein appA
UniRef100_P42061
Bacillus subtilis
AppA



precursor [Bacillus subtilis]


1151
Oligopeptide transport system
UniRef100_P42062
Bacillus subtilis
AppB



permease protein appB [Bacillus



subtilis]


1152
Oligopeptide transport system
UniRef100_P42063
Bacillus subtilis
AppC



permease protein appC [Bacillus



subtilis]


1153
Permease, putative [Bacillus cereus]
UniRef100_Q734Y3
Bacillus cereus
YvqJ


1154



YjbA


1155
Tryptophanyl-tRNA synthetase
UniRef100_P21656
Bacillus subtilis
TrpS



[Bacillus subtilis]


1156
Oligopeptide-binding protein oppA
UniRef100_P24141
Bacillus subtilis
OppA



precursor [Bacillus subtilis]


1157
Oligopeptide transport system
UniRef100_P24138
Bacillus subtilis
OppB



permease protein oppB [Bacillus



subtilis]


1158
Oligopeptide transport system
UniRef100_P24139
Bacillus subtilis
OppC



permease protein oppC [Bacillus



subtilis]


1159
Oligopeptide transport ATP-binding
UniRef100_P24136
Bacillus subtilis
OppD



protein oppD [Bacillus subtilis]


1160
Oligopeptide transport ATP-binding
UniRef100_P24137
Bacillus subtilis
OppF



protein oppF [Bacillus subtilis]


1161
YjbC protein [Bacillus subtilis]
UniRef100_O31601
Bacillus subtilis
YjbC


1162
Regulatory protein spx [Bacillus
UniRef100_O31602
Bacillus subtilis
YjbD



subtilis]


1163
YjbE protein [Bacillus subtilis]
UniRef100_O31603
Bacillus subtilis
YjbE


1164
Adapter protein mecA 1 [Bacillus
UniRef100_P37958
Bacillus subtilis
MecA



subtilis]


1165
Hypothetical conserved protein
UniRef100_Q8ELH8
Oceanobacillus
YflP



[Oceanobacillus iheyensis]

iheyensis










1166
Hypothetical protein OB3248
UniRef100_Q8ELH9
Oceanobacillus iheyensis



[Oceanobacillus iheyensis]


1167
Hypothetical conserved protein
UniRef100_Q8ELI0
Oceanobacillus iheyensis



[Oceanobacillus iheyensis]











1168
Response regulator of citrate/malate
UniRef100_Q7ML23
Vibrio vulnificus
CitT



metabolism [Vibrio vulnificus]


1169
Sensor protein citS [Bacillus
UniRef100_Q9RC53
Bacillus
YufL



halodurans]

halodurans


1170
YjbF protein [Bacillus subtilis]
UniRef100_O31604
Bacillus subtilis
YjbF


1171



YjbG


1172


1173
YjbH protein [Bacillus subtilis]
UniRef100_O31606
Bacillus subtilis
YjbH


1174
YjbI protein [Bacillus subtilis]
UniRef100_O31607
Bacillus subtilis
YjbI


1175
YjbJ protein [Bacillus subtilis]
UniRef100_O31608
Bacillus subtilis
YjbJ


1176
YjbK protein [Bacillus subtilis]
UniRef100_O31609
Bacillus subtilis
YjbK


1177
YjbL protein [Bacillus subtilis]
UniRef100_O31610
Bacillus subtilis
YjbL


1178
YjbM protein [Bacillus subtilis]
UniRef100_O31611
Bacillus subtilis
YjbM


1179



YjbN


1180
Hypothetical pseudouridine synthase
UniRef100_O31613
Bacillus subtilis
YjbO



yjbO [Bacillus subtilis]


1181
YjbP protein [Bacillus subtilis]
UniRef100_O31614
Bacillus subtilis
YjbP


1182
YjbQ protein [Bacillus subtilis]
UniRef100_O31615
Bacillus subtilis
YjbQ


1183
Transcriptional activator tenA [Bacillus
UniRef100_P25052
Bacillus subtilis
TenA



subtilis]


1184
Regulatory protein tenI [Bacillus
UniRef100_P25053
Bacillus subtilis
TenI



subtilis]


1185
Glycine oxidase [Bacillus subtilis]
UniRef100_O31616
Bacillus subtilis
GoxB


1186
ThiS protein [Bacillus subtilis]
UniRef100_O31617
Bacillus subtilis


1187
Thiazole biosynthesis protein thiG
UniRef100_O31618
Bacillus subtilis
ThiG



[Bacillus subtilis]


1188
ThiF protein [Bacillus subtilis]
UniRef100_O31619
Bacillus subtilis
ThiF


1189
YjbV protein [Bacillus subtilis]
UniRef100_O31620
Bacillus subtilis
YjbV


1190
Enoyl-[acyl-carrier-protein] reductase
UniRef100_P54616
acyl-carrier-
FabI



[NADH] [Bacillus subtilis]

protein


1191
YjbX protein [Bacillus subtilis]
UniRef100_O31622
Bacillus subtilis
YjbX


1192
Spore coat protein Z [Bacillus subtilis]
UniRef100_Q08312
Bacillus subtilis
CotZ


1193
Spore coat protein Y [Bacillus subtilis]
UniRef100_Q08311
Bacillus subtilis
CotY


1194
Spore coat protein X [Bacillus subtilis]
UniRef100_Q08313
Bacillus subtilis
CotX


1195
Spore coat protein W [Bacillus subtilis]
UniRef100_Q08310
Bacillus subtilis
CotW


1196
Spore coat protein V [Bacillus subtilis]
UniRef100_Q08309
Bacillus subtilis
CotV


1197
YjcA protein [Bacillus subtilis]
UniRef100_O31623
Bacillus subtilis
YjcA


1198


1199


1200
YjcC protein [Bacillus subtilis]
UniRef100_O31625
Bacillus subtilis


1201



YjcD


1202



YngC


1203



GalE


1204
YngB protein [Bacillus subtilis]
UniRef100_O31822
Bacillus subtilis
YngB


1205
YngA protein [Bacillus
UniRef100_Q70JY6
Bacillus
YngA



amyloliquefaciens]

amyloliquefaciens


1206
YjcF protein [Bacillus subtilis]
UniRef100_O31628
Bacillus subtilis
YjcF


1207
YjcG protein [Bacillus subtilis]
UniRef100_O31629
Bacillus subtilis
YjcG


1208
YjcH protein [Bacillus subtilis]
UniRef100_O31630
Bacillus subtilis
YjcH


1209
Hypothetical protein [Bacillus cereus]
UniRef100_Q739H9
Bacillus cereus


1210
BH1889 protein [Bacillus halodurans]
UniRef100_Q9KBN6
Bacillus
YobV





halodurans


1211
YjcI protein [Bacillus subtilis]
UniRef100_O31631
Bacillus subtilis
YjcI


1212
YjcJ protein [Bacillus subtilis]
UniRef100_O31632
Bacillus subtilis
YjcJ


1213
YjcL protein [Bacillus subtilis]
UniRef100_O31634
Bacillus subtilis
YjcL










1214
Transcriptional regulator, MarR/EmrR
UniRef100_Q97DR6
Clostridium acetobutylicum



family [Clostridium acetobutylicum]











1215
Penicillin-binding protein 4* [Bacillus
UniRef100_P32959
Bacillus subtilis
PbpE



subtilis]


1216



AbnA


1217


1218
Maltose transacetylase [Bacillus
UniRef100_Q75TH6
Bacillus
Maa



stearothermophilus]

stearothermophilus


1219


1220
Putative HTH-type transcriptional
UniRef100_P39647
Bacillus subtilis
YwfK



regulator ywfK [Bacillus subtilis]


1221
Sulfite reductase [Bacillus halodurans]
UniRef100_Q9KF76
Bacillus
YvgR





halodurans


1222
Sulfite reductase [Bacillus halodurans]
UniRef100_Q9KF75
Bacillus
YvgQ





halodurans


1223
Putative HTH-type transcriptional
UniRef100_O34701
Bacillus subtilis
YoaU



regulator yoaU [Bacillus subtilis]


1224
Hypothetical transport protein yoaV
UniRef100_O34416
Bacillus subtilis
YoaV



[Bacillus subtilis]


1225
Hypothetical protein VPA0302 [Vibrio
UniRef100_Q87JF1
Vibrio
YyaH



parahaemolyticus]

parahaemolyticus


1226
Hypothetical protein yoeB precursor
UniRef100_O34841
Bacillus subtilis
YoeB



[Bacillus subtilis]


1227



YocH


1228
Permease, general substrate
UniRef100_Q6HMC3
Bacillus
LmrB



transporter [Bacillus thuringiensis]

thuringiensis


1229
Putative HTH-type transcriptional
UniRef100_P42105
Bacillus subtilis
YxaF



regulator yxaF [Bacillus subtilis]


1230


1231



YmzD


1232



YeeF


1233



YjqB


1234
Phage-like element PBSX protein xkdA
UniRef100_P39780
Bacillus subtilis
XkdA



[Bacillus subtilis]


1235
HTH-type transcriptional regulator xre
UniRef100_P23789
Bacillus subtilis
Xre



[Bacillus subtilis]


1236


1237


1238
Phage-like element PBSX protein xkdB
UniRef100_P39781
Bacillus subtilis
XkdB



[Bacillus subtilis]


1239
Phage-like element PBSX protein xkdC
UniRef100_P39782
Bacillus subtilis
XkdC



[Bacillus subtilis]


1240
Phage-like element PBSX protein xkdD
UniRef100_P39783
Bacillus subtilis
XkdD



[Bacillus subtilis]


1241
Phage-like element PBSX protein xtrA
UniRef100_P54344
Bacillus subtilis



[Bacillus subtilis]


1242
Positive control factor [Bacillus subtilis]
UniRef100_P39784
Bacillus subtilis
Xpf


1243
PBSX phage terminase small subunit
UniRef100_P39785
Bacillus subtilis
XtmA



[Bacillus subtilis]


1244
PBSX phage terminase large subunit
UniRef100_P39786
Bacillus subtilis
XtmB



[Bacillus subtilis]


1245
Phage-like element PBSX protein xkdE
UniRef100_P54325
Bacillus subtilis
XkdE



[Bacillus subtilis]


1246
Phage-like element PBSX protein xkdF
UniRef100_P54326
Bacillus subtilis
XkdF



[Bacillus subtilis]


1247
Phage-like element PBSX protein xkdG
UniRef100_P54327
Bacillus subtilis
XkdG



[Bacillus subtilis]


1248
Hypothetical protein yqbG [Bacillus
UniRef100_P45923
Bacillus subtilis
YqbG



subtilis]


1249
Hypothetical protein yqbH [Bacillus
UniRef100_P45924
Bacillus subtilis
YqbH



subtilis]


1250
Phage-like element PBSX protein xkdI
UniRef100_P54329
Bacillus subtilis
XkdI



[Bacillus subtilis]


1251
Phage-like element PBSX protein xkdJ
UniRef100_P54330
Bacillus subtilis
XkdJ



[Bacillus subtilis]


1252
Lin1277 protein [Listeria innocua]
UniRef100_Q92CB2
Listeria innocua


1253
Phage-like element PBSX protein xkdK
UniRef100_P54331
Bacillus subtilis
XkdK



[Bacillus subtilis]


1254
Phage-like element PBSX protein xkdM
UniRef100_P54332
Bacillus subtilis
XkdM



[Bacillus subtilis]


1255
Phage-like element PBSX protein xkdN
UniRef100_P54333
Bacillus subtilis
XkdN



[Bacillus subtilis]


1256
Phage-like element PBSX protein xkdO
UniRef100_P54334
Bacillus subtilis
XkdO



[Bacillus subtilis]


1257
Phage-like element PBSX protein xkdP
UniRef100_P54335
Bacillus subtilis
YqbP



[Bacillus subtilis]


1258
Hypothetical protein yqbQ [Bacillus
UniRef100_P45950
Bacillus subtilis
YqbO



subtilis]


1259
Hypothetical protein yqbR [Bacillus
UniRef100_P45933
Bacillus subtilis
YqbR



subtilis]


1260
Phage-like element PBSX protein xkdS
UniRef100_P54338
Bacillus subtilis
XkdS



[Bacillus subtilis]


1261
Hypothetical protein yqbT [Bacillus
UniRef100_P45935
Bacillus subtilis
YqbT



subtilis]


1262
Phage-like element PBSX protein xkdU
UniRef100_P54340
Bacillus subtilis
XkdU



[Bacillus subtilis]


1263


1264



XkdV


1265



YomR


1266


1267


1268



BlyA


1269
Regulatory protein [Bacillus
UniRef100_Q9ZFL9
Bacillus
YdhC



stearothermophilus]

stearothermophilus


1270
Zinc-containing alcohol dehydrogenase
UniRef100_O35045
Bacillus subtilis
YjmD



[Bacillus subtilis]


1271
Mannonate dehydratase 1 [Bacillus
UniRef100_Q9KDZ8
Bacillus
UxuA



halodurans]

halodurans


1272
D-mannonate oxidoreductase [Bacillus
UniRef100_Q9KDZ4
Bacillus
YjmF



halodurans]

halodurans










1273
UPI00002F2634 UniRef100 entry
UniRef100_UPI00002F2634
YjmD











1274
Hexuronate transporter [Bacillus
UniRef100_O34456
Bacillus subtilis
ExuT



subtilis]


1275
Stage II sporulation protein SB
UniRef100_O34800
Bacillus subtilis



[Bacillus subtilis]


1276
Stage II sporulation protein SA
UniRef100_O34853
Bacillus subtilis
SpoIISA



[Bacillus subtilis]










1277
UPI00003CC121 UniRef100 entry
UniRef100_UPI00003CC121
Pit











1278
Hypothetical UPF0111 protein ykaA
UniRef100_O34454
Bacillus subtilis
YkaA



[Bacillus subtilis]


1279



Ggt


1280
YesL protein [Bacillus subtilis]
UniRef100_O31515
Bacillus subtilis
YesL


1281
YesM protein [Bacillus subtilis]
UniRef100_O31516
Bacillus subtilis
YesM


1282
YesN protein [Bacillus subtilis]
UniRef100_O31517
Bacillus subtilis
YesN


1283
YesO protein [Bacillus subtilis]
UniRef100_O31518
Bacillus subtilis
YesO


1284
Probable ABC transporter permease
UniRef100_O31519
Bacillus subtilis
YesP



protein yesP [Bacillus subtilis]


1285
Probable ABC transporter permease
UniRef100_O31520
Bacillus subtilis
YesQ



protein yesQ [Bacillus subtilis]


1286
YesR protein [Bacillus subtilis]
UniRef100_O31521
Bacillus subtilis
YesR


1287
YesS protein [Bacillus subtilis]
UniRef100_O31522
Bacillus subtilis
YesS


1288
YesT protein [Bacillus subtilis]
UniRef100_O31523
Bacillus subtilis
YesT


1289



YesU


1290
YesV protein [Bacillus subtilis]
UniRef100_O31525
Bacillus subtilis
YesV


1291
YesW protein [Bacillus subtilis]
UniRef100_O31526
Bacillus subtilis
YesW


1292


1293



YesT


1294
YesX protein [Bacillus subtilis]
UniRef100_O31527
Bacillus subtilis
YesX


1295
Putative ion-channel protein
UniRef100_Q8Z4X6
Salmonella typhi
YccK



[Salmonella typhi]


1296
YesY protein [Bacillus subtilis]
UniRef100_O31528
Bacillus subtilis
YesY


1297
YesZ protein [Bacillus subtilis]
UniRef100_O31529
Bacillus subtilis
YesZ


1298



YetA


1299
Lipoprotein lplA precursor [Bacillus
UniRef100_P37966
Bacillus subtilis
LplA



subtilis]


1300
LplB protein [Bacillus subtilis]
UniRef100_P39128
Bacillus subtilis
LplB


1301
LplC protein [Bacillus subtilis]
UniRef100_P39129
Bacillus subtilis
LplC


1302
YkbA protein [Bacillus subtilis]
UniRef100_O34739
Bacillus subtilis
YkbA


1303
YkcA protein [Bacillus subtilis]
UniRef100_O34689
Bacillus subtilis
YkcA


1304
Hypothetical protein [Bacillus cereus]
UniRef100_Q81CP9
Bacillus cereus


1305
Probable serine protease do-like htrA
UniRef100_O34358
Bacillus subtilis
HtrA



[Bacillus subtilis]


1306
Pyrroline-5-carboxylate reductase 3
UniRef100_Q00777
Bacillus subtilis
ProG



[Bacillus subtilis]


1307
D-aminopeptidase [Bacillus subtilis]
UniRef100_P26902
Bacillus subtilis
DppA


1308
Dipeptide transport system permease
UniRef100_P26903
Bacillus subtilis
DppB



protein dppB [Bacillus subtilis]


1309
Dipeptide transport system permease
UniRef100_P26904
Bacillus subtilis
DppC



protein dppC [Bacillus subtilis]


1310
Dipeptide transport ATP-binding
UniRef100_P26905
Bacillus subtilis
DppD



protein dppD [Bacillus subtilis]


1311
Dipeptide-binding protein dppE
UniRef100_P26906
Bacillus subtilis
DppE



precursor [Bacillus subtilis]


1312
Hypothetical protein ykfA [Bacillus
UniRef100_O34851
Bacillus subtilis
YkfA



subtilis]


1313
YkfB [Bacillus subtilis]
UniRef100_O34508
Bacillus subtilis
YkfB


1314
YkfC [Bacillus subtilis]
UniRef100_O35010
Bacillus subtilis
YkfC


1315
YkfD [Bacillus subtilis]
UniRef100_O34480
Bacillus subtilis
YkfD


1316
BH1779 protein [Bacillus halodurans]
UniRef100_Q9KBZ5
Bacillus
YkgA





halodurans


1317
Putative acyl-CoA thioester hydrolase
UniRef100_P49851
Bacillus subtilis
YkhA



ykhA [Bacillus subtilis]


1318



YkjA


1319
Pectate lyase 47 precursor [Bacillus sp.
UniRef100_Q9AJM4
Bacillus sp. TS-
Pel



TS-47]

47










1320
Transcriptional regulator, PadR family
UniRef100_Q6HED5
Bacillus thuringiensis



[Bacillus thuringiensis]


1321
Hypothetical protein OB0568
UniRef100_Q8ESQ3
Oceanobacillus iheyensis



[Oceanobacillus iheyensis]


1322
BH1312 protein [Bacillus halodurans]
UniRef100_Q9KDA2
Bacillus halodurans











1323
Hypothetical protein ykkA [Bacillus
UniRef100_P49854
Bacillus subtilis
YkkA



subtilis]


1324
Hypothetical protein ykkC [Bacillus
UniRef100_P49856
Bacillus subtilis
YkkC



subtilis]


1325


1326
YkkE [Bacillus subtilis]
UniRef100_O34990
Bacillus subtilis
YkkE


1327
Glutamate 5-kinase 1 [Bacillus subtilis]
UniRef100_P39820
Bacillus subtilis
ProB


1328
Gamma-glutamyl phosphate reductase
UniRef100_P39821
Bacillus subtilis
ProA



[Bacillus subtilis]


1329
Organic hydroperoxide resistance
UniRef100_O34762
Bacillus subtilis
YklA



protein ohrA [Bacillus subtilis]


1330
Organic hydroperoxide resistance
UniRef100_O34777
Bacillus subtilis
YkmA



transcriptional regulator [Bacillus



subtilis]


1331
Organic hydroperoxide resistance
UniRef100_P80242
Bacillus subtilis
YkzA



protein ohrB [Bacillus subtilis]


1332


1333
Guanine deaminase [Bacillus subtilis]
UniRef100_O34598
Bacillus subtilis
GuaD


1334
Phosphoglycerate mutase [Bacillus
UniRef100_Q9ALU0
Bacillus
YhfR



stearothermophilus]

stearothermophilus


1335


1336


1337
5-methyltetrahydropteroyltriglutamate-
UniRef100_P80877
Bacillus subtilis
MetE



homocysteine methyltransferase



[Bacillus subtilis]


1338
Intracellular serine protease [Bacillus
UniRef100_Q69DB4
Bacillus sp.
IspA



sp. WRD-2]

WRD-2


1339


1340
YkoK [Bacillus subtilis]
UniRef100_O34442
Bacillus subtilis
YkoK


1341


1342
Integrase [Oceanobacillus iheyensis]
UniRef100_Q8ETV2
Oceanobacillus
YdcL





iheyensis


1343



YqaB










1344
Hypothetical protein [Bacillus anthracis]
UniRef100_Q81UE0
Bacillus anthracis











1345



YonS


1346


1347
Putative HTH-type transcriptional
UniRef100_P45902
Bacillus subtilis
YqaE



regulator yqaE [Bacillus subtilis]










1348
Transcriptional regulator
UniRef100_Q7P886
Fusobacterium nucleatum



[Fusobacterium nucleatum subsp.

subsp. vincentii ATCC 49256



vincentii ATCC 49256]











1349






1350


1351
Lin1236 protein [Listeria innocua]
UniRef100_Q92CD6
Listeria innocua


1352


1353


1354


1355



YqaJ


1356
35 protein [Bacteriophage SPP1]
UniRef100_Q38143
Bacteriophage
YqaK





SPP1


1357



YqaL


1358



YqaM


1359
Hypothetical protein yqaO [Bacillus
UniRef100_P45912
Bacillus subtilis



subtilis]










1360
Hypothetical protein yopY
UniRef100_O64108
Bacteriophage SPBc2



[Bacteriophage SPBc2]











1361






1362


1363


1364
Hypothetical protein CTC02137
UniRef100_Q892G2
Clostridium



[Clostridium tetani]

tetani


1365
Hypothetical protein MW1918
UniRef100_Q8NVN5
Staphylococcus
YqaN



[Staphylococcus aureus]

aureus


1366










1367
Single-strand binding protein 2 [Listeria
UniRef100_Q8Y4X1
Listeria monocytogenes



monocytogenes]











1368






1369


1370
Hypothetical protein yqaQ [Bacillus
UniRef100_P45948
Bacillus subtilis
YqaQ



subtilis]


1371


1372
Hypothetical protein yqaS [Bacillus
UniRef100_P45915
Bacillus subtilis
YqaS



subtilis]


1373
Hypothetical protein yqaT [Bacillus
UniRef100_P45916
Bacillus subtilis
YqaT



subtilis]










1374
Hypothetical phage associated protein
UniRef100_Q8K6I0
Streptococcus pyogenes



SpyM3_1326 [Streptococcus



pyogenes]


1375
Minor head structural component GP7
UniRef100_O38442
Bacteriophage SPP1



[Bacteriophage SPP1]











1376






1377


1378


1379
Hypothetical protein CTC01553
UniRef100_Q894J0
Clostridium



[Clostridium tetani]

tetani










1380
Major capsid protein [Bacteriophage
UniRef100_Q9T1B7
Bacteriophage A118



A118]


1381
ORF28 [Bacteriophage phi-105]
UniRef100_Q9ZXF5
Bacteriophage phi-105


1382


1383
15 protein [Bacteriophage SPP1]
UniRef100_Q38584
Bacteriophage SPP1


1384
Complete nucleotide sequence
UniRef100_O48446
Bacteriophage SPP1



[Bacteriophage SPP1]


1385


1386
Complete nucleotide sequence
UniRef100_O48448
Bacteriophage SPP1



[Bacteriophage SPP1]


1387
Complete nucleotide sequence
UniRef100_O48449
Bacteriophage SPP1



[Bacteriophage SPP1]


1388


1389
Complete nucleotide sequence
UniRef100_O48453
Bacteriophage SPP1



[Bacteriophage SPP1]


1390











1391
Complete nucleotide sequence
UniRef100_O48455
Bacteriophage
XkdO



[Bacteriophage SPP1]

SPP1










1392
Complete nucleotide sequence
UniRef100_O48459
Bacteriophage SPP1



[Bacteriophage SPP1]


1393
Complete nucleotide sequence
UniRef100_O48463
Bacteriophage SPP1



[Bacteriophage SPP1]


1394


1395


1396
LycA [Clostridium botulinum]
UniRef100_Q6RI00
Clostridium botulinum











1397
Hypothetical protein yrkC [Bacillus
UniRef100_P54430
Bacillus subtilis
YrkC



subtilis]


1398



YhjR


1399
YdfS protein [Bacillus subtilis]
UniRef100_P96697
Bacillus subtilis
YdfS


1400
HTH-type transcriptional regulator tnrA
UniRef100_Q45666
Bacillus subtilis
TnrA



[Bacillus subtilis]


1401
Hypothetical protein ykzB [Bacillus
UniRef100_O34923
Bacillus subtilis



subtilis]


1402


1403
YkoM [Bacillus subtilis]
UniRef100_O34949
Bacillus subtilis
YkoM


1404
YkoU protein [Bacillus subtilis]
UniRef100_O34398
Bacillus subtilis
YkoU


1405
YkoV protein [Bacillus subtilis]
UniRef100_O34859
Bacillus subtilis
YkoV


1406
Signaling protein ykoW [Bacillus
UniRef100_O34311
Bacillus subtilis
YkoW



subtilis]


1407
YkoX protein [Bacillus subtilis]
UniRef100_O34908
Bacillus subtilis
YkoX


1408
YkoY protein [Bacillus subtilis]
UniRef100_O34997
Bacillus subtilis
YkoY


1409
RNA polymerase sigma factor [Bacillus
UniRef100_O31654
Bacillus subtilis
SigI



subtilis]


1410



YkrI










1411
Small, acid-soluble spore protein C3
UniRef100_P10572
Bacillus megaterium



[Bacillus megaterium]











1412
YkrK protein [Bacillus subtilis]
UniRef100_O31656
Bacillus subtilis
YkrK


1413
Probable protease htpX homolog
UniRef100_O31657
Bacillus subtilis
YkrL



[Bacillus subtilis]


1414
YkrM protein [Bacillus subtilis]
UniRef100_O31658
Bacillus subtilis
YkrM


1415
Penicillin-binding protein 3 [Bacillus
UniRef100_P42971
Bacillus subtilis
PbpC



subtilis]


1416
Hypothetical protein [Bacillus cereus]
UniRef100_Q73BI4
Bacillus cereus


1417


1418
YkrP protein [Bacillus subtilis]
UniRef100_O31660
Bacillus subtilis
YkrP


1419
Two-component sensor histidine
UniRef100_O31661
Bacillus subtilis
KinE



kinase [Bacillus subtilis]


1420
Methylated-DNA--protein-cysteine
UniRef100_P11742
Bacillus subtilis
Ogt



methyltransferase [Bacillus subtilis]


1421


1422
Methylthioribose-1-phosphate
UniRef100_O31662
Bacillus subtilis
YkrS



isomerase [Bacillus subtilis]


1423
Methylthioribose kinase [Bacillus
UniRef100_O31663
Bacillus subtilis
YkrT



subtilis]


1424
YkrU protein [Bacillus subtilis]
UniRef100_O31664
Bacillus subtilis
YkrU


1425
Transaminase mtnE [Bacillus subtilis]
UniRef100_O31665
Bacillus subtilis
YkrV


1426
2,3-diketo-5-methylthiopentyl-1-
UniRef100_O31666
Bacillus subtilis
YkrW



phosphate enolase [Bacillus subtilis]


1427
Methylthioribulose-1-phosphate
UniRef100_O31668
Bacillus subtilis
YkrY



dehydratase [Bacillus subtilis]


1428
1,2-dihydroxy-3-keto-5-
UniRef100_O31669
Bacillus subtilis
YkrZ



methylthiopentene dioxygenase



[Bacillus subtilis]


1429
Metallothiol transferase fosB
UniRef100_Q8CXK5
Oceanobacillus
YndN



[Oceanobacillus iheyensis]

iheyensis


1430
YkvA protein [Bacillus subtilis]
UniRef100_O31670
Bacillus subtilis


1431
Stage 0 sporulation regulatory protein
UniRef100_P05043
Bacillus subtilis



[Bacillus subtilis]


1432
Two-component sensor histidine
UniRef100_O31671
Bacillus subtilis
KinD



kinase [Bacillus subtilis]


1433



YkvE


1434
Chemotaxis motB protein [Bacillus
UniRef100_P28612
Bacillus subtilis
MotB



subtilis]


1435
Chemotaxis motA protein [Bacillus
UniRef100_P28611
Bacillus subtilis
MotA



subtilis]


1436
ATP-dependent Clp protease-like
UniRef100_O31673
Bacillus subtilis
ClpE



[Bacillus subtilis]


1437
YkvI protein [Bacillus subtilis]
UniRef100_O31674
Bacillus subtilis
YkvI


1438
YkvJ protein [Bacillus subtilis]
UniRef100_O31675
Bacillus subtilis
YkvJ


1439
YkvK protein [Bacillus subtilis]
UniRef100_O31676
Bacillus subtilis
YkvK


1440
YkvL protein [Bacillus subtilis]
UniRef100_O31677
Bacillus subtilis
YkvL


1441
YkvM protein [Bacillus subtilis]
UniRef100_O31678
Bacillus subtilis
YkvM


1442
DNA integration/recombination protein
UniRef100_Q894H7
Clostridium
CodV



[Clostridium tetani]

tetani


1443
Integrase/recombinase [Bacillus cereus
UniRef100_Q633V7
Bacillus cereus
RipX



ZK]

ZK


1444


1445


1446


1447
YoqV protein [Bacteriophage SPBc2]
UniRef100_O64130
Bacteriophage
LigB





SPBc2


1448


1449










1450
UPI00003CC586 UniRef100 entry
UniRef100_UPI00003CC586











1451





1452


1453


1454


1455
Prophage LambdaBa02, HNH
UniRef100_Q81W86
Bacillus anthracis



endonuclease family protein [Bacillus



anthracis]


1456
Terminase small subunit
UniRef100_Q6GAL5
Staphylococcus aureus



[Staphylococcus aureus]


1457
Prophage LambdaBa02, terminase,
UniRef100_Q6HUD2
Bacillus anthracis



large subunit, putative [Bacillus



anthracis]


1458
Hypothetical protein [Bacillus anthracis]
UniRef100_Q81W89
Bacillus anthracis











1459
ClpP family serine protease, possible
UniRef100_Q97HW4
Clostridium
ClpP



phage related [Clostridium

acetobutylicum



acetobutylicum]










1460
Prophage LambdaBa02, major capsid
UniRef100_Q81W91
Bacillus anthracis



protein, putative [Bacillus anthracis]


1461
Precursor polypeptide (AA-37 to 1647)
UniRef100_O03658
unidentified bacterium



precursor [unidentified bacterium]


1462
Gp7 protein [Bacteriophage phi3626]
UniRef100_Q8SBP7
Bacteriophage phi3626


1463
Uncharacterized phage related protein
UniRef100_Q97HW7
Clostridium acetobutylicum



[Clostridium acetobutylicum]


1464
Hypothetical protein CAC1887
UniRef100_Q97HW9
Clostridium acetobutylicum



[Clostridium acetobutylicum]


1465
Prophage LambdaBa02, major tail
UniRef100_Q81W97
Bacillus anthracis



protein, putative [Bacillus anthracis]











1466






1467


1468
Prophage LambdaBa02, tape measure
UniRef100_Q81WA0
Bacillus
YqbO



protein, putative [Bacillus anthracis]

anthracis


1469
Lin2382 protein [Listeria innocua]
UniRef100_Q928Z8
Listeria innocua










1470
Protein gp18 [Listeria monocytogenes]
UniRef100_Q8Y4Z4
Listeria monocytogenes











1471



YclG


1472



XkdV


1473



XkdW










1474
YomP protein [Bacteriophage SPBc2]
UniRef100_O64052
Bacteriophage SPBc2











1475
Glycerophosphoryl diester
UniRef100_Q737E6
Bacillus cereus
GlpQ



phosphodiesterase, putative [Bacillus



cereus]










1476
Protein bhlA [Bacteriophage SPBc2]
UniRef100_O64039
Bacteriophage SPBc2











1477
ORF46 [Bacteriophage phi-105]
UniRef100_Q9ZXD7
Bacteriophage
XlyB





phi-105


1478


1479


1480


1481
Transcriptional regulator, DeoR family
UniRef100_Q816D5
Bacillus cereus



[Bacillus cereus]










1482
Hypothetical protein yolD
UniRef100_O64030
Bacteriophage SPBc2



[Bacteriophage SPBc2]











1483
DNA
UniRef100_Q81GD4
Bacillus cereus
YdcL



integration/recombination/invertion



protein [Bacillus cereus]


1484
YkvM protein [Bacillus subtilis]
UniRef100_O31678
Bacillus subtilis


1485
Response regulator aspartate
UniRef100_P40771
Bacillus subtilis
RapH



phosphatase H [Bacillus subtilis]


1486
YoaT [Bacillus subtilis]
UniRef100_O34535
Bacillus subtilis
YoaT


1487
YozG protein [Bacillus subtilis]
UniRef100_O31834
Bacillus subtilis


1488
YoaS protein [Bacillus subtilis]
UniRef100_O31833
Bacillus subtilis
YoaS


1489


1490


1491


1492


1493


1494
YkvS protein [Bacillus subtilis]
UniRef100_O31684
Bacillus subtilis










1495
BH2327 protein [Bacillus halodurans]
UniRef100_Q9KAG0
Bacillus halodurans











1496
YkvT protein [Bacillus subtilis]
UniRef100_O31685
Bacillus subtilis
YkvT


1497
YkvU protein [Bacillus subtilis]
UniRef100_O31686
Bacillus subtilis
YkvU


1498
YkvV protein [Bacillus subtilis]
UniRef100_O31687
Bacillus subtilis
YkvV


1499



YkvW


1500
YkvY protein [Bacillus subtilis]
UniRef100_O31689
Bacillus subtilis
YkvY










1501
Necrosis and ethylene inducing protein
UniRef100_Q9KFT2
Bacillus halodurans



[Bacillus halodurans]











1502
Putative HTH-type transcriptional
UniRef100_O31690
Bacillus subtilis
YkvZ



regulator ykvZ [Bacillus subtilis]


1503
Transcription antiterminator [Bacillus
UniRef100_O06710
Bacillus subtilis
GlcT



subtilis]


1504



PtsG


1505
Phosphocarrier protein HPr [Bacillus
UniRef100_P08877
Bacillus subtilis



subtilis]


1506
Phosphoenolpyruvate-protein
UniRef100_P08838
Bacillus subtilis
PtsI



phosphotransferase [Bacillus subtilis]










1507
SplA [Bacillus amyloliquefaciens]
UniRef100_O54358
Bacillus amyloliquefaciens











1508
Spore photoproduct lyase [Bacillus
UniRef100_O54359
Bacillus
SplB



amyloliquefaciens]

amyloliquefaciens


1509
Hypothetical protein orf1 [Bacillus
UniRef100_O05187
Bacillus subtilis
YkwB



subtilis]


1510
Methyl-accepting chemotaxis protein
UniRef100_P54576
Bacillus subtilis
McpC



mcpC [Bacillus subtilis]


1511
3-oxoacyl-(Acyl carrier protein)
UniRef100_Q8EMP9
Oceanobacillus
FabG



reductase [Oceanobacillus iheyensis]

iheyensis


1512
Hypothetical oxidoreductase ykwC
UniRef100_O34948
Bacillus subtilis
YkwC



[Bacillus subtilis]


1513
YkwD protein [Bacillus subtilis]
UniRef100_O31694
Bacillus subtilis
YkwD


1514
YkuA protein [Bacillus subtilis]
UniRef100_O31399
Bacillus subtilis
YkuA


1515
Sporulation kinase A [Bacillus subtilis]
UniRef100_P16497
Bacillus subtilis
KinA


1516
Putative aminotransferase A [Bacillus
UniRef100_P16524
Bacillus subtilis
PatA



subtilis]


1517


1518
Hypothetical protein yxaI [Bacillus
UniRef100_P42108
Bacillus subtilis
YxaI



subtilis]


1519



YxiO


1520
Chemotaxis protein cheV [Bacillus
UniRef100_P37599
Bacillus subtilis
CheV



subtilis]


1521
Hypothetical protein ykyB [Bacillus
UniRef100_P42430
Bacillus subtilis
YkyB



subtilis]


1522



YkuC


1523
YkuD protein [Bacillus subtilis]
UniRef100_O34816
Bacillus subtilis
YkuD


1524



YkuE


1525
Hypothetical oxidoreductase ykuF
UniRef100_O34717
Bacillus subtilis
YkuF



[Bacillus subtilis]


1526
YkuI protein [Bacillus subtilis]
UniRef100_O35014
Bacillus subtilis
YkuI


1527


1528
YkuJ protein [Bacillus subtilis]
UniRef100_O34588
Bacillus subtilis


1529
YkuK protein [Bacillus subtilis]
UniRef100_O34776
Bacillus subtilis
YkuK


1530
Hypothetical protein ykzF [Bacillus
UniRef100_O31697
Bacillus subtilis



subtilis]


1531
YkuL protein [Bacillus subtilis]
UniRef100_O31698
Bacillus subtilis
YkuL


1532
Putative HTH-type transcriptional
UniRef100_O34827
Bacillus subtilis
CcpC



regulator ykuM [Bacillus subtilis]


1533
Probable flavodoxin 1 [Bacillus subtilis]
UniRef100_O34737
Bacillus subtilis
YkuN


1534
YkuO protein [Bacillus subtilis]
UniRef100_O34879
Bacillus subtilis
YkuO


1535
Probable flavodoxin 2 [Bacillus subtilis]
UniRef100_O34589
Bacillus subtilis
YkuP


1536
YkuQ protein [Bacillus subtilis]
UniRef100_O34981
Bacillus subtilis
YkuQ


1537
YkuR protein [Bacillus subtilis]
UniRef100_O34916
Bacillus subtilis
YkuR


1538
Hypothetical UPF0180 protein ykuS
UniRef100_O34783
Bacillus subtilis



[Bacillus subtilis]


1539
YkuU protein [Bacillus subtilis]
UniRef100_O34564
Bacillus subtilis
YkuU


1540
YkuV protein [Bacillus subtilis]
UniRef100_O31403
Bacillus subtilis
YkuV


1541
Repressor rok [Bacillus subtilis]
UniRef100_O34857
Bacillus subtilis
Rok


1542
YknT protein [Bacillus subtilis]
UniRef100_O31700
Bacillus subtilis
YknT


1543



MobA


1544
Molybdopterin biosynthesis protein
UniRef100_O31702
Bacillus subtilis
MoeB



MoeB [Bacillus subtilis]


1545
Molybdopterin biosynthesis protein
UniRef100_O31703
Bacillus subtilis
MoeA



MoeA [Bacillus subtilis]


1546
Molybdopterin-guanine dinucleotide
UniRef100_O31704
Bacillus subtilis
MobB



biosynthesis protein B [Bacillus subtilis]


1547
Molybdopterin converting factor, subunit
UniRef100_O31705
Bacillus subtilis
MoaE



2 [Bacillus subtilis]


1548
Molybdopterin converting factor,
UniRef100_O31706
Bacillus subtilis



subunit 1 [Bacillus subtilis]


1549


1550
YknU protein [Bacillus subtilis]
UniRef100_O31707
Bacillus subtilis
YknU


1551
YknV protein [Bacillus subtilis]
UniRef100_O31708
Bacillus subtilis
YknV


1552
Hypothetical protein yknW [Bacillus
UniRef100_O31709
Bacillus subtilis
YknW



subtilis]


1553
YknX protein [Bacillus subtilis]
UniRef100_O31710
Bacillus subtilis
YknX


1554
YknY protein [Bacillus subtilis]
UniRef100_O31711
Bacillus subtilis
YknY


1555
Hypothetical protein yknZ [Bacillus
UniRef100_O31712
Bacillus subtilis
YknZ



subtilis]


1556



FruR


1557
1-phosphofructokinase [Bacillus
UniRef100_O31714
Bacillus subtilis
FruK



subtilis]


1558
Phosphotransferase system (PTS)
UniRef100_P71012
Bacillus subtilis
FruA



fructose-specific enzyme IIABC



component [Bacillus subtilis]


1559
Signal peptidase I T [Bacillus subtilis]
UniRef100_P71013
Bacillus subtilis
SipT


1560
Hypothetical protein ykoA [Bacillus
UniRef100_O31715
Bacillus subtilis



subtilis]


1561


1562
YkpA protein [Bacillus subtilis]
UniRef100_O31716
Bacillus subtilis
YkpA










1563
BH1921 protein [Bacillus halodurans]
UniRef100_Q9KBK5
Bacillus halodurans











1564
Aminopeptidase ampS [Bacillus
UniRef100_P39762
Bacillus subtilis
AmpS



subtilis]


1565


1566
MreBH protein [Bacillus subtilis]
UniRef100_P39763
Bacillus subtilis
MreBH


1567


1568
Sporulation kinase C [Bacillus subtilis]
UniRef100_P39764
Bacillus subtilis
KinC


1569
Hypothetical protein ykqB [Bacillus
UniRef100_P39760
Bacillus subtilis
YkqB



subtilis]


1570
Adenine deaminase [Bacillus subtilis]
UniRef100_P39761
Bacillus subtilis
AdeC


1571



YkqC


1572
YkzG protein [Bacillus subtilis]
UniRef100_O31718
Bacillus subtilis


1573
Hypothetical protein ykrA [Bacillus
UniRef100_Q45494
Bacillus subtilis
YkrA



subtilis]


1574



YkrB


1575


1576
Hypothetical protein ykyA [Bacillus
UniRef100_P21884
Bacillus subtilis
YkyA



subtilis]


1577
Pyruvate dehydrogenase E1
UniRef100_P21881
Bacillus subtilis
PdhA



component, alpha subunit [Bacillus



subtilis]


1578
Pyruvate dehydrogenase E1
UniRef100_P21882
Bacillus subtilis
PdhB



component, beta subunit [Bacillus



subtilis]


1579
Dihydrolipoyllysine-residue
UniRef100_P21883
Bacillus subtilis
PdhC



acetyltransferase component of



pyruvate dehydrogenase complex



[Bacillus subtilis]


1580
Dihydrolipoyl dehydrogenase [Bacillus
UniRef100_P21880
Bacillus subtilis
PdhD



subtilis]










1581
UPI00003CC069 UniRef100 entry
UniRef100_UPI00003CC069











1582
IS1627s1-related, transposase
UniRef100_Q7CMD0
Bacillus anthracis str. A2012



[Bacillus anthracis str. A2012]











1583






1584


1585
Arginine decarboxylase [Bacillus
UniRef100_P21885
Bacillus subtilis
SpeA



subtilis]


1586
Hypothetical UPF0223 protein yktA
UniRef100_Q45497
Bacillus subtilis



[Bacillus subtilis]


1587
Hypothetical protein yktB [Bacillus
UniRef100_Q45498
Bacillus subtilis
YktB



subtilis]


1588
YkzI protein [Bacillus subtilis]
UniRef100_O31719
Bacillus subtilis


1589
Inositol-1-monophosphatase [Bacillus
UniRef100_Q45499
Bacillus subtilis
YktC



subtilis]


1590
Hypothetical protein ykzC [Bacillus
UniRef100_O31720
Bacillus subtilis
YkzC



subtilis]


1591
Hypothetical protein ylaA [Bacillus
UniRef100_O07625
Bacillus subtilis
YlaA



subtilis]


1592
Hypothetical protein ylaB [Bacillus
UniRef100_O07626
Bacillus subtilis



subtilis]


1593
YlaC protein [Bacillus subtilis]
UniRef100_O07627
Bacillus subtilis
YlaC


1594
Hypothetical protein ylaD [Bacillus
UniRef100_O07628
Bacillus subtilis



subtilis]


1595
Hypothetical protein ylaF [Bacillus
UniRef100_O07630
Bacillus subtilis



subtilis]


1596
GTP-binding protein typA/bipA
UniRef100_O07631
Bacillus subtilis
YlaG



homolog [Bacillus subtilis]


1597
YlaH protein [Bacillus subtilis]
UniRef100_O07632
Bacillus subtilis
YlaH


1598
YhzA homolog [Bacillus subtilis]
UniRef100_O07562
Bacillus subtilis
YhjH


1599
Hypothetical protein yhjG [Bacillus
UniRef100_O07561
Bacillus subtilis
YhjG



subtilis]


1600


1601
Hypothetical lipoprotein ylaJ precursor
UniRef100_O07634
Bacillus subtilis
YlaJ



[Bacillus subtilis]


1602
YlaK protein [Bacillus subtilis]
UniRef100_O07635
Bacillus subtilis
YlaK










1603
UPI00003CB7B1 UniRef100 entry
UniRef100_UPI00003CB7B1
YlaL











1604
Probable glutaminase ylaM [Bacillus
UniRef100_O07637
Bacillus subtilis
YlaM



subtilis]


1605
YlaN protein [Bacillus subtilis]
UniRef100_O07638
Bacillus subtilis


1606
Hypothetical protein ylaO [Bacillus
UniRef100_O07639
Bacillus subtilis
FtsW



subtilis]


1607



PycA


1608
Cytochrome AA3 controlling protein
UniRef100_P12946
Bacillus subtilis
CtaA



[Bacillus subtilis]


1609
Protoheme IX farnesyltransferase
UniRef100_P24009
Bacillus subtilis
CtaB



[Bacillus subtilis]


1610
Cytochrome c oxidase polypeptide II
UniRef100_P24011
Bacillus subtilis
CtaC



precursor (EC 1.9.3.1) (Cytochrome



aa3 subunit 2) (Caa-3605 subunit 2)



(Oxidase aa(3) subunit 2) [Bacillus



subtilis]


1611
Cytochrome c oxidase polypeptide I
UniRef100_P24010
Bacillus subtilis
CtaD



(EC 1.9.3.1) (Cytochrome aa3 subunit



1) (Caa-3605 subunit 1) (Oxidase aa(3)



subunit 1) [Bacillus subtilis]


1612
Cytochrome c oxidase polypeptide III
UniRef100_P24012
Bacillus subtilis
CtaE



(EC 1.9.3.1) (Cytochrome aa3 subunit



3) (Caa-3605 subunit 3) (Oxidase aa(3)



subunit 3) [Bacillus subtilis]


1613
Cytochrome c oxidase polypeptide IVB
UniRef100_P24013
Bacillus subtilis
CtaF



[Bacillus subtilis]


1614
CtaG protein [Bacillus subtilis]
UniRef100_O34329
Bacillus subtilis
CtaG


1615
YlbA protein [Bacillus subtilis]
UniRef100_O34743
Bacillus subtilis
YlbA


1616
YlbB protein [Bacillus subtilis]
UniRef100_O34682
Bacillus subtilis
YlbB


1617
YlbC protein [Bacillus subtilis]
UniRef100_O34586
Bacillus subtilis
YlbC


1618
YlbD protein [Bacillus subtilis]
UniRef100_O34880
Bacillus subtilis
YlbD


1619
YlbE protein [Bacillus subtilis]
UniRef100_O34958
Bacillus subtilis


1620
Regulatory protein ylbF [Bacillus
UniRef100_O34412
Bacillus subtilis
YlbF



subtilis]


1621
Hypothetical UPF0298 protein ylbG
UniRef100_O34658
Bacillus subtilis



[Bacillus subtilis]


1622
YlbH protein [Bacillus subtilis]
UniRef100_O34331
Bacillus subtilis
YlbH


1623
Phosphopantetheine
UniRef100_O34797
Bacillus subtilis
YlbI



adenylyltransferase [Bacillus subtilis]


1624



YlbJ


1625
YlbL protein [Bacillus subtilis]
UniRef100_O34470
Bacillus subtilis
YlbL


1626
YlbM protein [Bacillus subtilis]
UniRef100_O34513
Bacillus subtilis
YlbM


1627
YlbN protein [Bacillus subtilis]
UniRef100_O34445
Bacillus subtilis
YlbN


1628
50S ribosomal protein L32 [Bacillus
UniRef100_O34687
Bacillus subtilis



subtilis]


1629
Hypothetical protein ylbO [Bacillus
UniRef100_O34549
Bacillus subtilis
YlbO



subtilis]


1630
YlbP protein [Bacillus subtilis]
UniRef100_O34468
Bacillus subtilis
YlbP


1631
Probable 2-dehydropantoate 2-
UniRef100_O34661
Bacillus subtilis
YlbQ



reductase [Bacillus subtilis]


1632



YllA


1633
Protein mraZ [Bacillus subtilis]
UniRef100_P55343
Bacillus subtilis
YllB


1634
S-adenosyl-methyltransferase mraW
UniRef100_Q07876
Bacillus subtilis
YlxA



[Bacillus subtilis]


1635



FtsL


1636
Penicillin-binding protein 2B [Bacillus
UniRef100_Q07868
Bacillus subtilis
PbpB



subtilis]


1637
Stage V sporulation protein D [Bacillus
UniRef100_Q03524
Bacillus subtilis
SpoVD



subtilis]


1638
UDP-N-acetylmuramoylalanyl-D-
UniRef100_Q03523
Bacillus subtilis
MurE



glutamate--2,6-diaminopimelate ligase



[Bacillus subtilis]


1639
Phospho-N-acetylmuramoyl-
UniRef100_Q03521
Bacillus subtilis
MraY



pentapeptide-transferase [Bacillus



subtilis]


1640
UDP-N-acetylmuramoylalanine--D-
UniRef100_Q03522
Bacillus subtilis
MurD



glutamate ligase [Bacillus subtilis]


1641
Stage V sporulation protein E [Bacillus
UniRef100_P07373
Bacillus subtilis
SpoVE



subtilis]


1642
UDP-N-acetylglucosamine--N-
UniRef100_P37585
Bacillus subtilis
MurG



acetylmuramyl-(pentapeptide)



pyrophosphoryl-undecaprenol N-



acetylglucosamine transferase



[Bacillus subtilis]


1643
UDP-N-acetylenolpyruvoylglucosamine
UniRef100_P18579
Bacillus subtilis
MurB



reductase [Bacillus subtilis]


1644



DivIB


1645



YlxW


1646



YlxX


1647



Sbp


1648



FtsA


1649
Cell division protein ftsZ [Bacillus
UniRef100_P17865
Bacillus subtilis
FtsZ



subtilis]


1650



Bpr


1651
Bacillopeptidase F precursor [Bacillus
UniRef100_P16397
Bacillus subtilis
Bpr



subtilis]


1652
Sporulation sigma-E factor processing
UniRef100_P13801
Bacillus subtilis
SpoIIGA



peptidase [Bacillus subtilis]


1653
RNA polymerase sigma-E factor
UniRef100_P06222
Bacillus subtilis
SigE



precursor [Bacillus subtilis]


1654
RNA polymerase sigma-G factor
UniRef100_P19940
Bacillus subtilis
SigG



[Bacillus subtilis]


1655
YlmA protein [Bacillus subtilis]
UniRef100_O31723
Bacillus subtilis
YlmA


1656


1657
YlmC protein [Bacillus subtilis]
UniRef100_O31725
Bacillus subtilis


1658
Hypothetical UPF0124 protein ylmD
UniRef100_O31726
Bacillus subtilis
YlmD



[Bacillus subtilis]


1659



YlmE


1660
YlmF protein [Bacillus subtilis]
UniRef100_O31728
Bacillus subtilis
YlmF


1661
YlmG protein [Bacillus subtilis]
UniRef100_O31729
Bacillus subtilis


1662
Minicell-associated protein [Bacillus
UniRef100_P71020
Bacillus subtilis
YlmH



subtilis]


1663
Minicell-associated protein DivIVA
UniRef100_P71021
Bacillus subtilis
DivIVA



[Bacillus subtilis]


1664
Isoleucyl-tRNA synthetase [Bacillus
UniRef100_Q45477
Bacillus subtilis
IleS



subtilis]


1665



YlyA


1666



LspA


1667
Hypothetical pseudouridine synthase
UniRef100_Q45480
Bacillus subtilis
YlyB



ylyB [Bacillus subtilis]


1668
PyrR bifunctional protein [Includes:
UniRef100_P39765
Includes:
PyrR



Pyrimidine operon regulatory protein;

Pyrimidine



Uracil phosphoribosyltransferase (EC

operon



2.4.2.9) (UPRTase)] [Bacillus subtilis]

regulatory





protein; Uracil





phosphoribosyltransferase (EC 2.4.2.9)





(UPRTase)


1669
Uracil permease [Bacillus subtilis]
UniRef100_P39766
Bacillus subtilis
PyrP


1670
Aspartate carbamoyltransferase
UniRef100_P05654
Bacillus subtilis
PyrB



[Bacillus subtilis]


1671
Dihydroorotase [Bacillus subtilis]
UniRef100_P25995
Bacillus subtilis
PyrC


1672
Carbamoyl-phosphate synthase,
UniRef100_P25993
Bacillus subtilis
PyrAA



pyrimidine-specific, small chain



[Bacillus subtilis]


1673
Carbamoyl-phosphate synthase,
UniRef100_P25994
Bacillus subtilis
PyrAB



pyrimidine-specific, large chain



[Bacillus subtilis]


1674
Dihydroorotate dehydrogenase
UniRef100_P25983
Bacillus subtilis
PyrK



electron transfer subunit [Bacillus



subtilis]


1675
Dihydroorotate dehydrogenase,
UniRef100_P25996
Bacillus subtilis
PyrD



catalytic subunit [Bacillus subtilis]


1676
Orotidine 5′-phosphate decarboxylase
UniRef100_P25971
Bacillus subtilis
PyrF



[Bacillus subtilis]


1677
Orotate phosphoribosyltransferase
UniRef100_P25972
Bacillus subtilis
PyrE



[Bacillus subtilis]


1678


1679



CysH


1680
YlnA protein [Bacillus subtilis]
UniRef100_O34734
Bacillus subtilis
CysP


1681
Sulfate adenylyltransferase [Bacillus
UniRef100_O34764
Bacillus subtilis
Sat



subtilis]


1682
Probable adenylyl-sulfate kinase
UniRef100_O34577
Bacillus subtilis
CysC



[Bacillus subtilis]


1683
Putative S-adenosyl L-methionine:
UniRef100_O34744
Bacillus subtilis
YlnD



uroporphyrinogen III methyltransferase



[Bacillus subtilis]


1684
Sirohydrochlorin ferrochelatase
UniRef100_O34632
Bacillus subtilis
YlnE



[Bacillus subtilis]


1685
YlnF protein [Bacillus subtilis]
UniRef100_O34813
Bacillus subtilis
YlnF


1686
Putative fibronectin-binding protein
UniRef100_O34693
Bacillus subtilis
YloA



[Bacillus subtilis]


1687
YloB protein [Bacillus subtilis]
UniRef100_O34431
Bacillus subtilis
YloB


1688
YloC protein [Bacillus subtilis]
UniRef100_O34441
Bacillus subtilis
YloC


1689
Hypothetical UPF0296 protein ylzA
UniRef100_Q7WY72
Bacillus subtilis



[Bacillus subtilis]


1690
Guanylate kinase [Bacillus subtilis]
UniRef100_O34328
Bacillus subtilis
Gmk


1691
DNA-directed RNA polymerase omega
UniRef100_O35011
Bacillus subtilis



chain [Bacillus subtilis]


1692
YloI protein [Bacillus subtilis]
UniRef100_O35033
Bacillus subtilis
YloI


1693
Primosomal protein N′ [Bacillus subtilis]
UniRef100_P94461
Bacillus subtilis
PriA


1694
Peptide deformylase 1 [Bacillus
UniRef100_P94462
Bacillus subtilis
Def



subtilis]


1695
Methionyl-tRNA formyltransferase
UniRef100_P94463
Bacillus subtilis
Fmt



[Bacillus subtilis]


1696
Ribosomal RNA small subunit
UniRef100_P94464
Bacillus subtilis
YloM



methyltransferase B (EC 2.1.1.—) (rRNA



(cytosine-C(5)-)-methyltransferase)



[Bacillus subtilis]


1697
Hypothetical UPF0063 protein yloN
UniRef100_O34617
Bacillus subtilis
YloN



[Bacillus subtilis]


1698
Protein phosphatase [Bacillus subtilis]
UniRef100_O34779
Bacillus subtilis
PrpC


1699
Probable serine/threonine-protein
UniRef100_O34507
Bacillus subtilis
PrkC



kinase yloP [Bacillus subtilis]


1700
Probable GTPase engC [Bacillus
UniRef100_O34530
Bacillus subtilis
YloQ



subtilis]


1701
Ribulose-phosphate 3-epimerase
UniRef100_O34557
Bacillus subtilis
Rpe



[Bacillus subtilis]


1702
YloS protein [Bacillus subtilis]
UniRef100_O34664
Bacillus subtilis
YloS


1703


1704
50S ribosomal protein L28 [Bacillus
UniRef100_P37807
Bacillus subtilis



subtilis]


1705
Hypothetical protein yloU [Bacillus
UniRef100_O34318
Bacillus subtilis
YloU



subtilis]


1706



YloV


1707
Probable L-serine dehydratase, beta
UniRef100_O34635
Bacillus subtilis
SdaAB



chain [Bacillus subtilis]


1708
Probable L-serine dehydratase, alpha
UniRef100_O34607
Bacillus subtilis
SdaAA



chain [Bacillus subtilis]


1709
ATP-dependent DNA helicase recG
UniRef100_O34942
Bacillus subtilis
RecG



[Bacillus subtilis]


1710
Transcription factor fapR [Bacillus
UniRef100_O34835
Bacillus subtilis
YlpC



subtilis]


1711
Fatty acid/phospholipid synthesis
UniRef100_P71018
Bacillus subtilis
PlsX



protein plsX [Bacillus subtilis]


1712
Malonyl CoA-acyl carrier protein
UniRef100_P71019
Bacillus subtilis
FabD



transacylase [Bacillus subtilis]


1713
3-oxoacyl-[acyl-carrier-protein]
UniRef100_P51831
acyl-carrier-
FabG



reductase [Bacillus subtilis]

protein


1714
Acyl carrier protein [Bacillus subtilis]
UniRef100_P80643
Bacillus subtilis


1715



Rnc


1716
Chromosome partition protein smc
UniRef100_P51834
Bacillus subtilis
Smc



[Bacillus subtilis]


1717



FtsY


1718


1719
Signal recognition particle protein
UniRef100_P37105
Bacillus subtilis
Ffh



[Bacillus subtilis]


1720
30S ribosomal protein S16 [Bacillus
UniRef100_P21474
Bacillus subtilis



subtilis]


1721


1722
YlqD protein [Bacillus subtilis]
UniRef100_O31739
Bacillus subtilis
YlqD


1723



RimM


1724



TrmD


1725
50S ribosomal protein L19 [Bacillus
UniRef100_O31742
Bacillus subtilis
RplS



subtilis]


1726
YlqF protein [Bacillus subtilis]
UniRef100_O31743
Bacillus subtilis
YlqF


1727
Ribonuclease HII [Bacillus subtilis]
UniRef100_O31744
Bacillus subtilis
RnhB


1728
YlqG protein [Bacillus subtilis]
UniRef100_O31745
Bacillus subtilis
YlqG


1729
YlqH protein [Bacillus subtilis]
UniRef100_O34867
Bacillus subtilis


1730
Succinyl-CoA synthetase beta chain
UniRef100_P80886
Bacillus subtilis
SucC



[Bacillus subtilis]


1731
Succinyl-CoA synthetase alpha chain
UniRef100_P80865
Bacillus subtilis
SucD



[Bacillus subtilis]


1732



Smf


1733



TopA


1734
Protein gid [Bacillus subtilis]
UniRef100_P39815
Bacillus subtilis
Gid


1735
Tyrosine recombinase xerC [Bacillus
UniRef100_P39776
Bacillus subtilis
CodV



subtilis]


1736
ATP-dependent protease hslV
UniRef100_P39070
Bacillus subtilis
ClpQ



precursor [Bacillus subtilis]


1737
ATP-dependent hsl protease ATP-
UniRef100_P39778
Bacillus subtilis
ClpY



binding subunit hslU [Bacillus subtilis]


1738
GTP-sensing transcriptional pleiotropic
UniRef100_P39779
Bacillus subtilis
CodY



repressor codY [Bacillus subtilis]


1739
Flagellar basal-body rod protein flgB
UniRef100_P24500
Bacillus subtilis
FlgB



[Bacillus subtilis]


1740
Flagellar basal-body rod protein flgC
UniRef100_P24501
Bacillus subtilis
FlgC



[Bacillus subtilis]


1741
Flagellar hook-basal body complex
UniRef100_P24502
Bacillus subtilis
FliE



protein fliE [Bacillus subtilis]


1742
Flagellar M-ring protein [Bacillus
UniRef100_P23447
Bacillus subtilis
FliF



subtilis]


1743
Flagellar motor switch protein fliG
UniRef100_P23448
Bacillus subtilis
FliG



[Bacillus subtilis]


1744
Probable flagellar assembly protein fliH
UniRef100_P23449
Bacillus subtilis
FliH



[Bacillus subtilis]


1745
Flagellum-specific ATP synthase
UniRef100_P23445
Bacillus subtilis
FliI



[Bacillus subtilis]


1746
Flagellar fliJ protein [Bacillus subtilis]
UniRef100_P20487
Bacillus subtilis
FliJ


1747
FlaA locus 22.9 kDa protein [Bacillus
UniRef100_P23454
Bacillus subtilis
YlxF



subtilis]


1748
Probable flagellar hook-length control
UniRef100_P23451
Bacillus subtilis
FliK



protein [Bacillus subtilis]


1749
FlaA locus hypothetical protein ylxG
UniRef100_P23455
Bacillus subtilis
YlxG



[Bacillus subtilis]


1750



FlgE










1751
BH2448 protein [Bacillus halodurans]
UniRef100_Q9KA42
Bacillus halodurans











1752
Flagellar fliL protein [Bacillus subtilis]
UniRef100_P23452
Bacillus subtilis
FliL


1753
Flagellar motor switch protein fliM
UniRef100_P23453
Bacillus subtilis
FliM



[Bacillus subtilis]


1754
Flagellar motor switch protein fliY
UniRef100_P24073
Bacillus subtilis
FliY



[Bacillus subtilis]


1755
Chemotaxis protein cheY homolog
UniRef100_P24072
Bacillus subtilis
CheY



[Bacillus subtilis]


1756
Flagellar biosynthetic protein fliZ
UniRef100_P35536
Bacillus subtilis
FliZ



precursor [Bacillus subtilis]


1757
Flagellar biosynthetic protein fliP
UniRef100_P35528
Bacillus subtilis
FliP



[Bacillus subtilis]


1758
Flagellar biosynthetic protein fliQ
UniRef100_P35535
Bacillus subtilis



[Bacillus subtilis]


1759
Flagellar biosynthetic protein fliR
UniRef100_P35537
Bacillus subtilis
FliR



[Bacillus subtilis]


1760
Flagellar biosynthetic protein flhB
UniRef100_P35538
Bacillus subtilis
FlhB



[Bacillus subtilis]


1761
Flagellar biosynthesis protein flhA
UniRef100_P35620
Bacillus subtilis
FlhA



[Bacillus subtilis]


1762
Flagellar biosynthesis protein flhF
UniRef100_Q01960
Bacillus subtilis
FlhF



[Bacillus subtilis]


1763
Hypothetical protein ylxH [Bacillus
UniRef100_P40742
Bacillus subtilis
YlxH



subtilis]


1764
Chemotaxis response regulator
UniRef100_Q05522
Bacillus subtilis
CheB



protein-glutamate methylesterase



[Bacillus subtilis]


1765
Chemotaxis protein cheA [Bacillus
UniRef100_P29072
Bacillus subtilis
CheA



subtilis]


1766
Chemotaxis protein cheW [Bacillus
UniRef100_P39802
Bacillus subtilis
CheW



subtilis]


1767
Chemotaxis protein cheC [Bacillus
UniRef100_P40403
Bacillus subtilis
CheC



subtilis]


1768
Chemotaxis protein cheD [Bacillus
UniRef100_P40404
Bacillus subtilis
CheD



subtilis]


1769
RNA polymerase sigma-D factor
UniRef100_P10726
Bacillus subtilis
SigD



[Bacillus subtilis]


1770
Swarming motility protein swrB
UniRef100_P40405
Bacillus subtilis
YlxL



[Bacillus subtilis]


1771
30S ribosomal protein S2 [Bacillus
UniRef100_P21464
Bacillus subtilis
RpsB



subtilis]


1772
Translation elongation factor Ts
UniRef100_Q65JJ8
Bacillus cereus
Tsf



[Bacillus cereus]


1773



PyrH


1774



Frr


1775
Undecaprenyl pyrophosphate
UniRef100_O31751
Bacillus subtilis
UppS



synthetase [Bacillus subtilis]


1776
Phosphatidate cytidylyltransferase
UniRef100_O31752
Bacillus subtilis
CdsA



[Bacillus subtilis]


1777
1-deoxy-D-xylulose 5-phosphate
UniRef100_O31753
Bacillus subtilis
Dxr



reductoisomerase [Bacillus subtilis]


1778
Hypothetical zinc metalloprotease yluc
UniRef100_O31754
Bacillus subtilis
YluC



[Bacillus subtilis]


1779
Prolyl-tRNA synthetase [Bacillus
UniRef100_O31755
Bacillus subtilis
ProS



subtilis]


1780
DNA polymerase III polC-type [Bacillus
UniRef100_P13267
Bacillus subtilis
PolC



subtilis]


1781










1782
Cellulose 1,4-beta-cellobiosidase
UniRef100_Q8KKF7
Paenibacillus sp. BP-23



precursor [Paenibacillus sp. BP-23]


1783
Endoglucanase B precursor
UniRef100_P23550
Paenibacillus lautus



[Paenibacillus lautus]


1784
Beta-mannosidase [Thermotoga
UniRef100_Q9RIK7
Thermotoga neapolitana



neapolitana]











1785
Hypothetical UPF0090 protein ylxS
UniRef100_P32726
Bacillus subtilis
YlxS



[Bacillus subtilis]


1786
Transcription elongation protein nusA
UniRef100_P32727
Bacillus subtilis
NusA



[Bacillus subtilis]


1787
Hypothetical protein ylxR [Bacillus
UniRef100_P32728
Bacillus subtilis



subtilis]


1788
Probable ribosomal protein ylxQ
UniRef100_P32729
Bacillus subtilis



[Bacillus subtilis]


1789
Translation initiation factor IF-2
UniRef100_P17889
Bacillus subtilis
InfB



[Bacillus subtilis]


1790
Hypothetical protein ylxP [Bacillus
UniRef100_P32730
Bacillus subtilis



subtilis]


1791
Ribosome-binding factor A [Bacillus
UniRef100_P32731
Bacillus subtilis
RbfA



subtilis]


1792



TruB


1793
Riboflavin biosynthesis protein ribC
UniRef100_P54575
Includes:
RibC



[Includes: Riboflavin kinase (EC

Riboflavin kinase



2.7.1.26) (Flavokinase); FMN

(EC 2.7.1.26)



adenylyltransferase (EC 2.7.7.2) (FAD

(Flavokinase);



pyrophosphorylase) (FAD synthetase)]

FMN



[Bacillus subtilis]

adenylyltransferase





(EC 2.7.7.2)





(FAD





pyrophosphorylase)





(FAD





synthetase)


1794
30S ribosomal protein S15 [Bacillus
UniRef100_P21473
Bacillus subtilis



subtilis]


1795
Polyribonucleotide
UniRef100_P50849
Bacillus subtilis
PnpA



nucleotidyltransferase [Bacillus subtilis]


1796
Hypothetical protein ylxY precursor
UniRef100_P50850
Bacillus subtilis
YlxY



[Bacillus subtilis]


1797
Hypothetical zinc protease ymxG
UniRef100_Q04805
Bacillus subtilis
MlpA



[Bacillus subtilis]


1798
Hypothetical protein ymxH [Bacillus
UniRef100_Q04811
Bacillus subtilis



subtilis]


1799
Dipicolinate synthase, A chain [Bacillus
UniRef100_Q04809
Bacillus subtilis
SpoVFA



subtilis]


1800
Dipicolinate synthase, B chain [Bacillus
UniRef100_Q04810
Bacillus subtilis
SpoVFB



subtilis]


1801
Aspartate-semialdehyde
UniRef100_Q04797
Bacillus subtilis
Asd



dehydrogenase [Bacillus subtilis]


1802
Aspartokinase 1 (EC 2.7.2.4)
UniRef100_Q04795
Contains:
DapG



(Aspartokinase I) (Aspartate kinase 1)

Aspartokinase I



[Contains: Aspartokinase I alpha

alpha subunit;



subunit; Aspartokinase I beta subunit]

Aspartokinase I



[Bacillus subtilis]

beta subunit


1803
Dihydrodipicolinate synthase [Bacillus
UniRef100_Q04796
Bacillus subtilis
DapA



subtilis]


1804
YmfA protein [Bacillus subtilis]
UniRef100_O31760
Bacillus subtilis
YmfA


1805
Translocation-enhancing protein tepA
UniRef100_Q99171
Bacillus subtilis
TepA



[Bacillus subtilis]


1806


1807
DNA translocase ftsK [Bacillus subtilis]
UniRef100_P21458
Bacillus subtilis
SpoIIIE


1808
Hypothetical transcriptional regulator
UniRef100_O31761
Bacillus subtilis
YmfC



ymfC [Bacillus subtilis]


1809
Multidrug resistance protein [Bacillus
UniRef100_Q9K7Q2
Bacillus
YitG



halodurans]

halodurans


1810
Hypothetical protein [Bacillus anthracis]
UniRef100_Q81WP6
Bacillus
YmfG





anthracis


1811
YmfH protein [Bacillus subtilis]
UniRef100_O31766
Bacillus subtilis
YmfH


1812


1813


1814
YmfJ protein [Bacillus subtilis]
UniRef100_O31768
Bacillus subtilis


1815
Hypothetical protein [Bacillus cereus
UniRef100_Q636P2
Bacillus cereus
YmfK



ZK]

ZK


1816



YmfM


1817
CDP-diacylglycerol--glycerol-3-
UniRef100_P46322
Bacillus subtilis
PgsA



phosphate 3-phosphatidyltransferase



[Bacillus subtilis]


1818
CinA-like protein [Bacillus subtilis]
UniRef100_P46323
Bacillus subtilis
CinA


1819
RecA protein [Bacillus
UniRef100_Q8GJG2
Bacillus
RecA



amyloliquefaciens]

amyloliquefaciens


1820
Hypothetical UPF0144 protein ymdA
UniRef100_O31774
Bacillus subtilis
YmdA



[Bacillus subtilis]


1821
YmdB protein [Bacillus subtilis]
UniRef100_O31775
Bacillus subtilis
YmdB


1822
Stage V sporulation protein S [Bacillus
UniRef100_P45693
Bacillus subtilis



subtilis]


1823


1824


1825
L-threonine 3-dehydrogenase [Bacillus
UniRef100_O31776
Bacillus subtilis
Tdh



subtilis]


1826
2-amino-3-ketobutyrate coenzyme A
UniRef100_O31777
Bacillus subtilis
Kbl



ligase [Bacillus subtilis]


1827
Hypothetical UPF0004 protein ymcB
UniRef100_O31778
Bacillus subtilis
YmcB



[Bacillus subtilis]


1828
YmcA protein [Bacillus subtilis]
UniRef100_O31779
Bacillus subtilis
YmcA


1829
Spore coat protein E [Bacillus subtilis]
UniRef100_P14016
Bacillus subtilis
CotE


1830



MutS


1831
DNA mismatch repair protein mutL
UniRef100_P49850
Bacillus subtilis
MutL



[Bacillus subtilis]


1832
YjcS protein [Bacillus subtilis]
UniRef100_O31641
Bacillus subtilis


1833



YxiD


1834


1835


1836
All1751 protein [Anabaena sp.]
UniRef100_Q8YW65
Anabaena sp.
YciC










1837
BH0367 protein [Bacillus halodurans]
UniRef100_Q9KFV4
Bacillus halodurans











1838
Phosphinothricin N-acetyltransferase
UniRef100_Q9KFP5
Bacillus
YwnH



[Bacillus halodurans]

halodurans










1839
UPI00003CC0D8 UniRef100 entry
UniRef100_UPI00003CC0D8



1840











1841
Putative L-amino acid oxidase [Bacillus
UniRef100_O34363
Bacillus subtilis
YobN



subtilis]


1842



YoaK


1843
Na+/H+ antiporter [Bacillus halodurans]
UniRef100_Q9K5Q0
Bacillus
YvgP





halodurans










1844
Aromatic hydrocarbon catabolism
UniRef100_Q8CV32
Oceanobacillus iheyensis



protein [Oceanobacillus iheyensis]


1845
Hypothetical UPF0145 protein VP1283
UniRef100_Q87Q67
Vibrio parahaemolyticus



[Vibrio parahaemolyticus]











1846
Hypothetical protein yqeD [Bacillus
UniRef100_P54449
Bacillus subtilis
YqeD



subtilis]


1847
Penicillin-binding protein, putative
UniRef100_Q738U9
Bacillus cereus
PbpE



[Bacillus cereus]


1848
Hypothetical glycosyl transferase
UniRef100_O34539
Bacillus subtilis
YjiC



[Bacillus subtilis]


1849
Asparate-proton symporter [Bacillus
UniRef100_O07002
Bacillus subtilis
YveA



subtilis]










1850
Spore coat protein [Bacillus
UniRef100_Q9KEV6
Bacillus halodurans



halodurans]











1851






1852



YdhD


1853
Putative HTH-type transcriptional
UniRef100_Q7WY76
Bacillus subtilis
YezE



regulator yezE [Bacillus subtilis]


1854
Hypothetical protein yesE [Bacillus
UniRef100_O31511
Bacillus subtilis
YesE



subtilis]


1855
YesF protein [Bacillus subtilis]
UniRef100_O31512
Bacillus subtilis
YesF










1856
UPI00003CBA3B UniRef100 entry
UniRef100_UPI00003CBA3B












1857
YmaD protein [Bacillus subtilis]
UniRef100_O31790
Bacillus subtilis
YmaD


1858
Multidrug resistance protein ebrB
UniRef100_O31791
Bacillus subtilis
EbrB



[Bacillus subtilis]


1859
Multidrug resistance protein ebrA
UniRef100_O31792
Bacillus subtilis
EbrA



[Bacillus subtilis]


1860


1861
Hypothetical protein ymaF [Bacillus
UniRef100_O31794
Bacillus subtilis
YmaF



subtilis]


1862
tRNA delta(2)-
UniRef100_O31795
Bacillus subtilis
MiaA



isopentenylpyrophosphate transferase



[Bacillus subtilis]


1863
Hfq protein [Bacillus subtilis]
UniRef100_O31796
Bacillus subtilis


1864
Hypothetical protein ymzA [Bacillus
UniRef100_O31798
Bacillus subtilis



subtilis]


1865
NrdI protein [Bacillus subtilis]
UniRef100_P50618
Bacillus subtilis
YmaA


1866
Ribonucleoside-diphosphate reductase
UniRef100_P50620
Bacillus subtilis
NrdE



alpha chain [Bacillus subtilis]


1867
Ribonucleoside-diphosphate reductase
UniRef100_P50621
Bacillus subtilis
NrdF



beta chain [Bacillus subtilis]


1868
Hypothetical protein ymaB [Bacillus
UniRef100_P50619
Bacillus subtilis
YmaB



subtilis]


1869
Blr6966 protein [Bradyrhizobium
UniRef100_Q89EV4
Bradyrhizobium
YtnP



japonicum]

japonicum


1870
Nitrogen fixation protein [Bacillus
UniRef100_Q9KFV2
Bacillus
YurV



halodurans]

halodurans


1871
Transcription regulator Fur family-like
UniRef100_Q8CNQ7
Staphylococcus
PerR



protein [Staphylococcus epidermidis]

epidermidis










1872
UPI00003CB681 UniRef100 entry
UniRef100_UPI00003CB681
YdhC











1873
Hypothetical protein [Bacillus cereus]
UniRef100_Q81C60
Bacillus cereus
YjlA


1874



CwlC










1875
Membrane protein, putative [Listeria
UniRef100_Q720L9
Listeria monocytogenes



monocytogenes]











1876
Lin1174 protein [Listeria innocua]
UniRef100_Q92CJ7
Listeria innocua



1877


1878


1879
Transcriptional regulator [Aquifex
UniRef100_O66635
Aquifex aeolicus
YdgC



aeolicus]










1880
Hypothetical membrane-anchored
UniRef100_Q92VA1
Rhizobium meliloti



protein [Rhizobium meliloti]











1881
Cytosine permease [Bacillus
UniRef100_Q9KBP3
Bacillus
YxlA



halodurans]

halodurans










1882
AgaF [Agrobacterium tumefaciens]
UniRef100_O50265
Agrobacterium tumefaciens


1883


1884
Hydantoin utilization protein B
UniRef100_Q88H51
Pseudomonas putida



[Pseudomonas putida]


1885
Hypothetical protein SMb20139
UniRef100_Q92X23
Rhizobium meliloti



[Rhizobium meliloti]


1886
BH2340 protein [Bacillus halodurans]
UniRef100_Q9KAE7
Bacillus halodurans


1887











1888






1889
Stage V sporulation protein K [Bacillus
UniRef100_P27643
Bacillus subtilis
SpoVK



subtilis]


1890
YnbA [Bacillus subtilis]
UniRef100_P94478
Bacillus subtilis
YnbA


1891
YnbB [Bacillus subtilis]
UniRef100_P94479
Bacillus subtilis
YnbB


1892
HTH-type transcriptional regulator glnR
UniRef100_P37582
Bacillus subtilis
GlnR



[Bacillus subtilis]


1893
Glutamine synthetase [Bacillus subtilis]
UniRef100_P12425
Bacillus subtilis
GlnA


1894










1895
Hypothetical protein CAC3435
UniRef100_Q97DN7
Clostridium acetobutylicum



[Clostridium acetobutylicum]


1896


1897


1898
Hypothetical protein CAC0350
UniRef100_Q97M50
Clostridium acetobutylicum



[Clostridium acetobutylicum]











1899
Hypothetical HIT-like protein MJ0866
UniRef100_Q58276
Methanococcus
Hit



[Methanococcus jannaschii]

jannaschii










1900
Hypothetical protein [Bacillus anthracis]
UniRef100_Q6I2B3
Bacillus anthracis











1901
Hypothetical protein [Bacillus cereus]
UniRef100_Q81CM1
Bacillus cereus
YdjC


1902


1903
Methyltransferase [Bacillus cereus]
UniRef100_Q81CJ2
Bacillus cereus


1904



YoaO


1905
Acetyltransferase, GNAT family
UniRef100_Q737B4
Bacillus cereus



[Bacillus cereus]


1906
Repressor rok [Bacillus subtilis]
UniRef100_O34857
Bacillus subtilis
Rok


1907










1908
Hypothetical protein [Bacteriophage
UniRef100_Q6QGK2
Bacteriophage T5



T5]











1909
Hypothetical protein yvdT [Bacillus
UniRef100_O07001
Bacillus subtilis
YvdT



subtilis]


1910
Hypothetical protein yvdS [Bacillus
UniRef100_O32262
Bacillus subtilis
YvdS



subtilis]


1911
Hypothetical protein yvdR [Bacillus
UniRef100_O06999
Bacillus subtilis
YvdR



subtilis]


1912
Spermidine N1-acetyltransferase
UniRef100_Q72Y03
Bacillus cereus
YoaA



[Bacillus cereus]


1913
Hypothetical Membrane Associated
UniRef100_Q812L6
Bacillus cereus



Protein [Bacillus cereus]


1914
Hypothetical protein yoaW precursor
UniRef100_O34541
Bacillus subtilis
YoaW



[Bacillus subtilis]


1915
Thiol-disulfide oxidoreductase resA
UniRef100_Q9KCJ4
Bacillus
ResA



[Bacillus halodurans]

halodurans


1916
Manganese-containing catalase
UniRef100_Q9KAU6
Bacillus
YdbD



[Bacillus halodurans]

halodurans










1917
BH1562 protein [Bacillus halodurans]
UniRef100_Q9KCK9
Bacillus halodurans











1918
Acetyltransferase, GNAT family
UniRef100_Q739K0
Bacillus cereus
YjcK



[Bacillus cereus]










1919
Hypothetical conserved protein
UniRef100_Q8ELR7
Oceanobacillus iheyensis



[Oceanobacillus iheyensis]











1920






1921
Transcriptional regulator, MarR family
UniRef100_Q81BM5
Bacillus cereus
YkvE



[Bacillus cereus]


1922
Putative NAD(P)H nitroreductase ydfN
UniRef100_P96692
Bacillus subtilis
YdfN



[Bacillus subtilis]


1923
YdfO protein [Bacillus subtilis]
UniRef100_P96693
Bacillus subtilis
YdfO


1924
Hypothetical protein [Bacillus cereus
UniRef100_Q630S7
Bacillus cereus
YwrF



ZK]

ZK


1925
BH1010 protein [Bacillus halodurans]
UniRef100_Q9KE48
Bacillus
YhcB





halodurans










1926
ORF28 [Staphylococcus phage K]
UniRef100_Q6Y7T8
Staphylococcus phage K











1927






1928
Thymidylate synthase [Bacteriophage
UniRef100_P07606
Bacteriophage
ThyA



phi-3T]

phi-3T


1929
Sporulation-specific extracellular
UniRef100_P42983
Bacillus subtilis
NucB



nuclease precursor [Bacillus subtilis]


1930
LexA repressor [Bacillus subtilis]
UniRef100_P31080
Bacillus subtilis
LexA


1931
YneA [Bacillus subtilis]
UniRef100_Q45056
Bacillus subtilis
YneA


1932
YneB [Bacillus subtilis]
UniRef100_Q45057
Bacillus subtilis
YneB


1933
Hypothetical UPF0291 protein ynzC
UniRef100_O31818
Bacillus subtilis



[Bacillus subtilis]


1934
Transketolase [Bacillus subtilis]
UniRef100_P45694
Bacillus subtilis
Tkt


1935
Hypothetical protein yneE [Bacillus
UniRef100_P45707
Bacillus subtilis
YneE



subtilis]


1936
Hypothetical UPF0154 protein yneF
UniRef100_P45708
Bacillus subtilis



[Bacillus subtilis]


1937


1938
Cytochrome c-type biogenesis protein
UniRef100_P45706
Bacillus subtilis
CcdA



ccdA [Bacillus subtilis]


1939
CcdB protein [Bacillus subtilis]
UniRef100_P45709
Bacillus subtilis
YneI


1940
CcdC protein [Bacillus subtilis]
UniRef100_P45710
Bacillus subtilis
YneJ


1941
Hypothetical protein yneK [Bacillus
UniRef100_P45711
Bacillus subtilis
YneK



subtilis]


1942
Spore coat protein M [Bacillus subtilis]
UniRef100_Q45058
Bacillus subtilis
CotM


1943


1944


1945



CitB


1946
YneN protein [Bacillus subtilis]
UniRef100_O31820
Bacillus subtilis
YneN


1947


1948


1949
Small, acid-soluble spore protein tlp
UniRef100_Q45060
Bacillus subtilis



[Bacillus subtilis]


1950
YneP [Bacillus subtilis]
UniRef100_Q45061
Bacillus subtilis
YneP


1951
YneQ [Bacillus subtilis]
UniRef100_Q45062
Bacillus subtilis


1952
Hypothetical protein [Bacillus cereus]
UniRef100_Q815P1
Bacillus cereus


1953
Conserved domain protein [Bacillus
UniRef100_Q72YR7
Bacillus cereus



cereus]


1954
YneR [Bacillus subtilis]
UniRef100_Q45063
Bacillus subtilis


1955
Hypothetical UPF0078 protein yneS
UniRef100_Q45064
Bacillus subtilis
YneS



[Bacillus subtilis]


1956
YneT [Bacillus subtilis]
UniRef100_Q45065
Bacillus subtilis
YneT


1957
Topoisomerase IV subunit B [Bacillus
UniRef100_Q59192
Bacillus subtilis
ParE



subtilis]


1958
Topoisomerase IV subunit A [Bacillus
UniRef100_Q45066
Bacillus subtilis
ParC



subtilis]


1959



AraR


1960
Hypothetical conserved protein
UniRef100_Q8EMP2
Oceanobacillus
XylB



[Oceanobacillus iheyensis]

iheyensis


1961
L-ribulose-5-phosphate 4-epimerase
UniRef100_Q8EMP3
Oceanobacillus
AraD



[Oceanobacillus iheyensis]

iheyensis


1962
L-arabinose isomerase
UniRef100_Q8EMP4
Oceanobacillus
AraA



[Oceanobacillus iheyensis]

iheyensis


1963



YwtG


1964



FabG


1965
Hypothetical protein ynfC [Bacillus
UniRef100_Q45067
Bacillus subtilis
YnfC



subtilis]


1966
Amino acid carrier protein alsT
UniRef100_Q45068
Bacillus subtilis
AlsT



[Bacillus subtilis]


1967



NarI


1968



NarJ


1969



NarH


1970



NarG


1971










1972
Hypothetical protein yqfB [Lactococcus
UniRef100_Q9CF70
Lactococcus lactis



lactis]











1973



AlbA


1974



ArfM


1975



YwiC


1976
Transcriptional regulator of anaerobic
UniRef100_Q9KG81
Bacillus
Fnr



genes [Bacillus halodurans]

halodurans


1977
Nitrite extrusion protein [Bacillus
UniRef100_P46907
Bacillus subtilis
NarK



subtilis]


1978


1979
CAMP-binding domains-Catabolite
UniRef100_Q8R5P4
Thermoanaerobacter
Fnr



gene activator and regulatory subunit

tengcongensis



of cAMP-dependent protein kinases



[Thermoanaerobacter tengcongensis]










1980
Putative nitric oxide reductase
UniRef100_Q6GK48
Staphylococcus aureus



[Staphylococcus aureus]











1981






1982



YngL


1983



BglC


1984
Hypothetical protein ynfE [Bacillus
UniRef100_Q45069
Bacillus subtilis



subtilis]


1985
Hypothetical protein [Bacillus
UniRef100_Q9ZF48
Bacillus
YkkB



megaterium]

megaterium










1986
Hypothetical protein [Bacillus
UniRef100_Q70K06
Bacillus amyloliquefaciens



amyloliquefaciens]











1987
Alkyl hydroperoxide reductase large
UniRef100_Q9Z9W3
Bacillus
AhpF



subunit [Bacillus halodurans]

halodurans










1988
Methyltransferase [Methanosarcina
UniRef100_Q8PU82
Methanosarcina mazei



mazei]











1989
Similar to B. subtilis ywgB gene
UniRef100_Q9Z9W2
Bacillus
YwgB



[Bacillus halodurans]

halodurans


1990
Hypothetical protein ywoF [Bacillus
UniRef100_P94576
Bacillus subtilis
YwoF



subtilis]


1991
Branched-chain amino acid transport
UniRef100_P94499
Bacillus subtilis
BrnQ



system carrier protein brnQ [Bacillus



subtilis]


1992
NADP-dependent alcohol
UniRef100_O06007
Bacillus subtilis
AdhA



dehydrogenase [Bacillus subtilis]


1993
Transcriptional regulator, MerR family
UniRef100_Q721Z3
Listeria
YraB



[Listeria monocytogenes]

monocytogenes


1994
HPr-like protein crh [Bacillus subtilis]
UniRef100_O06976
Bacillus subtilis


1995
BH2089 protein [Bacillus halodurans]
UniRef100_Q9Z9R4
Bacillus
YddR





halodurans


1996


1997
Enoyl-CoA hydratase/isomerase family
UniRef100_Q738L0
Bacillus cereus
YngF



protein [Bacillus cereus]


1998
Hypothetical protein ysiB [Bacillus
UniRef100_P94549
Bacillus subtilis
YsiB



subtilis]


1999
Methylmalonic acid semialdehyde
UniRef100_Q63BL0
Bacillus cereus
MmsA



dehydrogenase [Bacillus cereus ZK]

ZK


2000
3-hydroxyisobutyrate dehydrogenase
UniRef100_Q63BL1
Bacillus cereus
YkwC



[Bacillus cereus ZK]

ZK


2001
Acyl-CoA dehydrogenase [Bacillus
UniRef100_Q81DR7
Bacillus cereus
YusJ



cereus]


2002
Mannose-6-phosphate isomerase
UniRef100_O31646
Bacillus subtilis
ManA



[Bacillus subtilis]


2003
Phosphotransferase system (PTS)
UniRef100_O31645
Bacillus subtilis
ManP



mannose-specific enzyme IIBCA



component [Bacillus subtilis]


2004


2005


2006
Hypothetical protein [Bacillus cereus]
UniRef100_Q72YT6
Bacillus cereus


2007
Transcriptional regulator [Bacillus
UniRef100_O31644
Bacillus subtilis
ManR



subtilis]


2008










2009
UPI00003CC220 UniRef100 entry
UniRef100_UPI00003CC220
YtrB











2010
Transcriptional regulator [Bacillus
UniRef100_Q9KF35
Bacillus
YtrA



halodurans]

halodurans


2011
Probable oxidoreductase [Clostridium
UniRef100_Q8XP17
Clostridium
YjmF



perfringens]

perfringens


2012
Mannonate dehydratase [Clostridium
UniRef100_Q8XP15
Clostridium
UxuA



perfringens]

perfringens










2013
Glucosidase [Bacillus halodurans]
UniRef100_Q9KEZ5
Bacillus halodurans


2014
C4-dicarboxylate transport system
UniRef100_Q9KEZ6
Bacillus halodurans



[Bacillus halodurans]


2015
C4-dicarboxylate transport system
UniRef100_Q8EMM5
Oceanobacillus iheyensis



permease small protein



[Oceanobacillus iheyensis]











2016
C4-dicarboxylate transport system
UniRef100_Q9KEZ8
Bacillus
DctB



[Bacillus halodurans]

halodurans


2017
Transcriptional regulator
UniRef100_Q8EL22
Oceanobacillus
CcpA



[Oceanobacillus iheyensis]

iheyensis


2018
Arsenate reductase [Bacillus subtilis]
UniRef100_P45947
Bacillus subtilis
ArsC


2019
YdfA protein [Bacillus subtilis]
UniRef100_P96678
Bacillus subtilis
YdfA


2020
YdeT protein [Bacillus subtilis]
UniRef100_P96677
Bacillus subtilis
YdeT


2021
YdeI [Bacillus halodurans]
UniRef100_Q9Z9R5
Bacillus
YdeI





halodurans










2022
Putative secreted protein
UniRef100_Q9S1Z5
Streptomyces coelicolor



[Streptomyces coelicolor]











2023
Probable glucose uptake protein glcU
UniRef100_P40419
Bacillus
GlcU



[Bacillus megaterium]

megaterium


2024
YngK protein [Bacillus subtilis]
UniRef100_O35015
Bacillus subtilis
YngK


2025
YngD protein [Bacillus subtilis]
UniRef100_O31824
Bacillus subtilis
YngD


2026
Pyruvate formate-lyase-activating
UniRef100_Q73DZ6
Bacillus cereus
YkvL



enzyme [Bacillus cereus]










2027
Formate acetyltransferase [Bacillus
UniRef100_Q81YX1
Bacillus anthracis



anthracis]











2028



DacC


2029
NADH dehydrogenase-like protein yjlD
UniRef100_P80861
Bacillus subtilis
YjlD



[Bacillus subtilis]


2030
Hypothetical protein yjlC [Bacillus
UniRef100_O34633
Bacillus subtilis
YjlC



subtilis]


2031


2032
Hypothetical protein [Bacillus cereus]
UniRef100_Q81IJ8
Bacillus cereus


2033
Hypothetical protein ykzH [Bacillus
UniRef100_O31653
Bacillus subtilis



subtilis]


2034
Acetyl-CoA synthetase [Bacillus
UniRef100_Q9KDS4
Bacillus
AcsA



halodurans]

halodurans


2035
YngE protein [Bacillus subtilis]
UniRef100_O31825
Bacillus subtilis
YngE


2036
Hydroxybutyryl-dehydratase [Bacillus
UniRef100_Q9L7W1
Bacillus subtilis
YngF



subtilis]


2037
YngG protein [Bacillus subtilis]
UniRef100_O34873
Bacillus subtilis
YngG


2038
YngXX [Bacillus subtilis]
UniRef100_Q9R9I3
Bacillus subtilis


2039
YngH [Bacillus subtilis]
UniRef100_Q9R9I4
Bacillus subtilis
YngH


2040
YngI [Bacillus subtilis]
UniRef100_Q9R9I5
Bacillus subtilis
YngI


2041
YngJ protein [Bacillus subtilis]
UniRef100_O34421
Bacillus subtilis
YngJ


2042
NAD(P)H oxidoreductase YRKL
UniRef100_Q7P6P0
Fusobacterium
YrkL



[Fusobacterium nucleatum subsp.

nucleatum



vincentii ATCC 49256]

subsp. vincentii





ATCC 49256










2043
Transcriptional regulator, MarR family
UniRef100_Q8RE85
Fusobacterium nucleatum



[Fusobacterium nucleatum]











2044
Glutamate-5-semialdehyde
UniRef100_Q6HHC2
Bacillus
ProA



dehydrogenase [Bacillus thuringiensis]

thuringiensis


2045
Glutamate 5-kinase 2 [Bacillus subtilis]
UniRef100_O07509
Bacillus subtilis
ProJ


2046
Pyrroline-5-carboxylate reductase 1
UniRef100_P14383
Bacillus subtilis
ProH



[Bacillus subtilis]










2047
UPI00003CB6CD UniRef100 entry
UniRef100_UPI00003CB6CD
YetF











2048
Sodium-dependent phosphate
UniRef100_Q9KCT1
Bacillus
CysP



transporter [Bacillus halodurans]

halodurans


2049
Probable phosphoadenosine
UniRef100_O06737
Bacillus subtilis
YitB



phosphosulfate reductase [Bacillus



subtilis]


2050
Phosphosulfolactate synthase (EC
UniRef100_O06739
Bacillus subtilis
YitD



4.4.1.19) ((2R)-phospho-3-sulfolactate



synthase) [Bacillus subtilis]


2051
YitE [Bacillus subtilis]
UniRef100_O06740
Bacillus subtilis
YitE


2052
YitF [Bacillus subtilis]
UniRef100_O06741
Bacillus subtilis
YitF


2053
YitG [Bacillus subtilis]
UniRef100_O06742
Bacillus subtilis
YitG


2054
Putative glycosyl transferase ykoT
UniRef100_O34755
Bacillus subtilis
YkoT



[Bacillus subtilis]


2055
YkoR [Bacillus subtilis]
UniRef100_O34830
Bacillus subtilis
YkoS


2056
Glutamate synthase [NADPH] small
UniRef100_O34399
NADPH
GltB



chain [Bacillus subtilis]


2057



GltA


2058
HTH-type transcriptional regulator gltC
UniRef100_P20668
Bacillus subtilis
GltC



[Bacillus subtilis]


2059
AIl7121 protein [Anabaena sp.]
UniRef100_Q8YL17
Anabaena sp.


2060










2061
Lmo0606 protein [Listeria
UniRef100_Q8Y9C6
Listeria monocytogenes



monocytogenes]











2062
ABC transporter ATP-binding protein
UniRef100_Q67MU2
Symbiobacterium
YfiB



[Symbiobacterium thermophilum]

thermophilum


2063
ABC transporter ATP-binding protein
UniRef100_Q67MU3
Symbiobacterium
YfiC



[Symbiobacterium thermophilum]

thermophilum


2064
Cytochrome P450 109 [Bacillus
UniRef100_P27632
Bacillus subtilis
YjiB



subtilis]


2065
Hypothetical oxidoreductase yoxD
UniRef100_P14802
Bacillus subtilis
YoxD



[Bacillus subtilis]


2066



Pps


2067
Permease, general substrate
UniRef100_Q6HMC3
Bacillus
LmrB



transporter [Bacillus thuringiensis]

thuringiensis


2068
Putative formate dehydrogenase
UniRef100_O34323
Bacillus subtilis
YoaE



[Bacillus subtilis]


2069
Transcriptional regulatory protein
UniRef100_Q89KD1
Bradyrhizobium
YcgE



[Bradyrhizobium japonicum]

japonicum


2070
Drug resistance transporter,
UniRef100_Q73615
Bacillus cereus
Mdr



EmrB/QacA family [Bacillus cereus]


2071
YndE protein [Bacillus subtilis]
UniRef100_O31809
Bacillus subtilis
YndE


2072
YndF protein [Bacillus subtilis]
UniRef100_O31810
Bacillus subtilis
YndF


2073



YndE


2074



YndE










2075
UPI00003CB22E UniRef100 entry
UniRef100_UPI00003CB22E
YndD











2076






2077
Nucleotide binding protein expZ
UniRef100_P39115
Bacillus subtilis
ExpZ



[Bacillus subtilis]


2078



TlpB


2079
CsaA protein [Bacillus subtilis]
UniRef100_P37584
Bacillus subtilis
CsaA


2080
Alkaline serine protease [Bacillus
UniRef100_O31788
Bacillus subtilis
AprX



subtilis]


2081
Hypothetical protein [Bacillus anthracis]
UniRef100_Q81SD4
Bacillus
YnzE





anthracis


2082
Transcriptional regulator, TetR family
UniRef100_Q63D70
Bacillus cereus
YrhI



[Bacillus cereus ZK]

ZK


2083
Putative HTH-type transcriptional
UniRef100_P40762
Bacillus subtilis
YvmB



regulator yvmB [Bacillus subtilis]


2084
Hydrolase [Bacillus cereus]
UniRef100_Q81D79
Bacillus cereus
YqeK










2085
Hypothetical conserved protein
UniRef100_Q8ETG3
Oceanobacillus iheyensis



[Oceanobacillus iheyensis]











2086
YndJ protein [Bacillus subtilis]
UniRef100_O31813
Bacillus subtilis
YndJ


2087
YndH protein [Bacillus subtilis]
UniRef100_O31812
Bacillus subtilis
YndH


2088
Hypothetical protein [Bacillus cereus]
UniRef100_Q73A97
Bacillus cereus
YndG










2089
UPI00003CBA97 UniRef100 entry
UniRef100_UPI00003CBA97
YobS


2090
UPI00003CBA98 UniRef100 entry
UniRef100_UPI00003CBA98
YobT











2091
DNA-binding protein YobU [Bacillus
UniRef100_O34637
Bacillus subtilis
YobU



subtilis]










2092
Hypothetical protein [Bacteroides
UniRef100_Q64RP1
Bacteroides fragilis



fragilis]











2093
Transcription regulator [Bacillus
UniRef100_O34920
Bacillus subtilis
YobV



subtilis]


2094



YobW


2095
YozA protein [Bacillus subtilis]
UniRef100_O31844
Bacillus subtilis


2096
Possible metallo-beta-lactamase family
UniRef100_Q638G1
Bacillus cereus
YmaE



protein [Bacillus cereus ZK]

ZK


2097


2098



YfnA


2099



YocA










2100
UPI00003CBE6E UniRef100 entry
UniRef100_UPI00003CBE6E
YveM











2101
Hypothetical protein [Bacillus cereus]
UniRef100_Q817C2
Bacillus cereus



2102


2103
Glycosyl transferase [Bacillus
UniRef100_Q9K7I1
Bacillus
YtcC



halodurans]

halodurans


2104
YozB protein [Bacillus subtilis]
UniRef100_O31845
Bacillus subtilis
YozB










2105
Lmo2079 protein [Listeria
UniRef100_Q8Y5I3
Listeria monocytogenes



monocytogenes]











2106
YocC [Bacillus subtilis]
UniRef100_O35042
Bacillus subtilis
YocC


2107
Na+/myo-inositol cotransporter
UniRef100_Q9KAR5
Bacillus
YcgO



[Bacillus halodurans]

halodurans


2108
YocH [Bacillus subtilis]
UniRef100_O34669
Bacillus subtilis
YocH


2109
RecQ homolog [Bacillus subtilis]
UniRef100_O34748
Bacillus subtilis
YocI


2110
Hypothetical protein yqbC [Bacillus
UniRef100_P45919
Bacillus subtilis
YqbC



subtilis]


2111
Hypothetical protein yjfB [Bacillus
UniRef100_O34438
Bacillus subtilis



subtilis]


2112
Hypothetical protein yyaQ [Bacillus
UniRef100_P37507
Bacillus subtilis
YyaQ



subtilis]










2113
Hypothetical protein OB2103
UniRef100_Q8EPJ8
Oceanobacillus iheyensis



[Oceanobacillus iheyensis]











2114






2115
Hypothetical protein yjgD [Bacillus
UniRef100_O34681
Bacillus subtilis
YjgD



subtilis]


2116



YjgC


2117



YjgC










2118
Hypothetical protein OB3361
UniRef100_Q8EL70
Oceanobacillus iheyensis



[Oceanobacillus iheyensis]











2119
Hypothetical protein ypfA [Bacillus
UniRef100_P38491
Bacillus subtilis
YpfA



subtilis]


2120


2121


2122



YxiB


2123


2124


2125


2126



YndF


2127


2128
Hypothetical protein [Bacillus cereus]
UniRef100_Q816C2
Bacillus cereus











2129
BH0185 protein [Bacillus halodurans]
UniRef100_Q9KGB9
Bacillus halodurans











2130






2131



YisY


2132
Hypothetical protein yoqH
UniRef100_O64117
Bacteriophage
YoqH



[Bacteriophage SPBc2]

SPBc2


2133


2134
BH0429 protein [Bacillus halodurans]
UniRef100_Q9KFP9
Bacillus
YrhP





halodurans










2135
30S ribosomal protein S14
UniRef100_Q8ETX0
Oceanobacillus iheyensis



[Oceanobacillus iheyensis]










2136
UPI00002DEBB5 UniRef100 entry
UniRef100_UPI00002DEBB5
MutT











2137
Pyruvate water dikinase
UniRef100_Q8TN35
Methanosarcina
Pps



[Methanosarcina acetivorans]

acetivorans


2138
Transcriptional regulator
UniRef100_Q8ESJ8
Oceanobacillus
YxbF



[Oceanobacillus iheyensis]

iheyensis


2139


2140
YndM protein [Bacillus subtilis]
UniRef100_O31816
Bacillus subtilis
YndM


2141
Hypothetical protein yisT [Bacillus
UniRef100_O07939
Bacillus subtilis
YisT



subtilis]


2142
Putative acyl carrier protein
UniRef100_O35022
Bacillus subtilis
YocJ



phosphodiesterase 1 [Bacillus subtilis]


2143
General stress protein 16O [Bacillus
UniRef100_P80872
Bacillus subtilis
YocK



subtilis]


2144


2145


2146
Aldehyde dehydrogenase [Bacillus
UniRef100_O34660
Bacillus subtilis
DhaS



subtilis]


2147
YjbB protein [Bacillus subtilis]
UniRef100_O31600
Bacillus subtilis
YjbB


2148
Aminoglycoside N6′-acetyltransferase
UniRef100_Q81AT3
Bacillus cereus
YjcK



[Bacillus cereus]










2149
Blasticidin S deaminase, putative
UniRef100_Q81Y61
Bacillus anthracis



[Bacillus anthracis]











2150



SqhC


2151
Probable superoxide dismutase [Fe]
UniRef100_O35023
Fe
SodF



[Bacillus subtilis]


2152
Stress response protein yvgO
UniRef100_O32211
Bacillus subtilis
YvgO



precursor [Bacillus subtilis]


2153
Putative transporter [Bacillus subtilis]
UniRef100_O34383
Bacillus subtilis
YocR


2154
Putative transporter [Bacillus subtilis]
UniRef100_O34524
Bacillus subtilis
YocS


2155
Dihydrolipoyllysine-residue
UniRef100_P16263
Bacillus subtilis
OdhB



succinyltransferase component of 2-



oxoglutarate dehydrogenase complex



[Bacillus subtilis]


2156
2-oxoglutarate dehydrogenase E1
UniRef100_P23129
Bacillus subtilis
OdhA



component [Bacillus subtilis]


2157
YojO protein [Bacillus subtilis]
UniRef100_O31849
Bacillus subtilis
YojO


2158
YojN protein [Bacillus subtilis]
UniRef100_O31850
Bacillus subtilis
YojN


2159
Hypothetical superoxide dismutase-like
UniRef100_O31851
Bacillus subtilis
YojM



protein yojM precursor [Bacillus



subtilis]


2160
Hypothetical protein yojL precursor
UniRef100_O31852
Bacillus subtilis
LytF



[Bacillus subtilis]


2161
Probable multidrug resistance protein
UniRef100_O31855
Bacillus subtilis
YojI



norM (Na(+)/drug antiporter) [Bacillus



subtilis]


2162
Hypothetical protein [Bacillus cereus
UniRef100_Q637Z8
Bacillus cereus
YojG



ZK]

ZK


2163
YojF protein [Bacillus subtilis]
UniRef100_O31858
Bacillus subtilis
YojF


2164
YojE [Bacillus subtilis]
UniRef100_O68260
Bacillus subtilis


2165
YojE protein [Bacillus subtilis]
UniRef100_O31859
Bacillus subtilis
YojE


2166
Hypothetical protein yozR [Bacillus
UniRef100_Q7WY67
Bacillus subtilis
YozR



subtilis]


2167
YoaJ [Bacillus subtilis]
UniRef100_O34918
Bacillus subtilis
YoaJ


2168










2169
UPI00003CBE9B UniRef100 entry
UniRef100_UPI00003CBE9B
YwfD











2170
Hypothetical UPF0087 protein yodB
UniRef100_O34844
Bacillus subtilis
YodB



[Bacillus subtilis]


2171
Putative NAD(P)H nitroreductase 12C
UniRef100_P81102
Bacillus subtilis
YodC



[Bacillus subtilis]


2172
YolF [Bacillus subtilis]
UniRef100_O34842
Bacillus subtilis
YodD










2173
UPI0000315ACC UniRef100 entry
UniRef100_UPI0000315ACC












2174
YodF protein [Bacillus subtilis]
UniRef100_O34745
Bacillus subtilis
YodF










2175
IS1627s1-related, transposase
UniRef100_Q7CMD0
Bacillus anthracis str. A2012



[Bacillus anthracis str. A2012]










2176
UPI00003CC069 UniRef100 entry
UniRef100_UPI00003CC069












2177
OrfRM1 [Bacillus subtilis]
UniRef100_O34666
Bacillus subtilis
CtpA


2178
YolB [Bacillus subtilis]
UniRef100_O34954
Bacillus subtilis
YodH


2179


2180
Carboxypeptidase [Bacillus subtilis]
UniRef100_O34866
Bacillus subtilis
YodJ


2181
Purine nucleoside phosphorylase II
UniRef100_O34925
Bacillus subtilis
DeoD



[Bacillus subtilis]


2182
Hypothetical Membrane Spanning
UniRef100_Q813P0
Bacillus cereus
YcgR



Protein [Bacillus cereus]


2183
YcgQ protein [Bacillus subtilis]
UniRef100_P94394
Bacillus subtilis
YcgQ


2184


2185
Hypothetical protein yodL [Bacillus
UniRef100_O30472
Bacillus subtilis
YodL



subtilis]


2186



YodM


2187
Hypothetical protein yozD [Bacillus
UniRef100_O31863
Bacillus subtilis



subtilis]


2188
Hypothetical protein yodN [Bacillus
UniRef100_O34414
Bacillus subtilis
YodN



subtilis]


2189


2190
YokU [Bacillus subtilis]
UniRef100_O30470
Bacillus subtilis


2191
Hypothetical UPF0069 protein yodO
UniRef100_O34676
Bacillus subtilis
KamA



[Bacillus subtilis]


2192
YodP [Bacillus subtilis]
UniRef100_O34895
Bacillus subtilis
YodP


2193
Acetylornitine deacetylase [Bacillus
UniRef100_O34984
Bacillus subtilis
YodQ



subtilis]


2194
Butirate-acetoacetate CoA transferase
UniRef100_O34466
Bacillus subtilis
YodR



[Bacillus subtilis]


2195
Butyrate acetoacetate-CoA transferase
UniRef100_O34317
Bacillus subtilis
YodS



[Bacillus subtilis]


2196
Probable aminotransferase yodT
UniRef100_O34662
Bacillus subtilis
YodT



[Bacillus subtilis]


2197
Multidrug resistance protein; possible
UniRef100_Q6HK46
Bacillus
YkuC



tetracycline resistance determinant

thuringiensis



[Bacillus thuringiensis]


2198
Protein cgeE [Bacillus subtilis]
UniRef100_P42093
Bacillus subtilis
CgeE


2199
Peptide methionine sulfoxide reductase
UniRef100_P54155
Bacillus subtilis
YppQ



msrB [Bacillus subtilis]


2200



MsrA


2201
Putative HTH-type transcriptional
UniRef100_P54182
Bacillus subtilis
YpoP



regulator ypoP [Bacillus subtilis]


2202


2203
Hypothetical protein yhcK [Bacillus
UniRef100_P54595
Bacillus subtilis
YhcK



subtilis]


2204
Hypothetical protein ypnP [Bacillus
UniRef100_P54181
Bacillus subtilis
YpnP



subtilis]










2205
Hypothetical conserved protein
UniRef100_Q746K9
Thermus thermophilus



[Thermus thermophilus]











2206






2207
Hypothetical protein ypmS [Bacillus
UniRef100_P54179
Bacillus subtilis
YpmS



subtilis]


2208
Hypothetical protein ypmR [Bacillus
UniRef100_P40766
Bacillus subtilis
YpmR



subtilis]


2209
Hypothetical protein ypmQ [Bacillus
UniRef100_P54178
Bacillus subtilis
YpmQ



subtilis]


2210
DegV family protein [Bacillus cereus
UniRef100_Q63BU6
Bacillus cereus
YviA



ZK]

ZK


2211
Hypothetical protein ypmP [Bacillus
UniRef100_P54177
Bacillus subtilis



subtilis]


2212
Threonine dehydratase biosynthetic
UniRef100_P37946
Bacillus subtilis
IlvA



[Bacillus subtilis]


2213
Putative sigma L-dependent
UniRef100_P54156
Bacillus subtilis
YplP



transcriptional regulator yplP [Bacillus



subtilis]


2214
Hemolysin III homolog [Bacillus
UniRef100_P54175
Bacillus subtilis
YplQ



subtilis]


2215
Hypothetical protein ypkP [Bacillus
UniRef100_P54174
Bacillus subtilis
YpkP



subtilis]


2216
Dihydrofolate reductase [Bacillus
UniRef100_P11045
Bacillus subtilis
DfrA



subtilis]


2217
Hypothetical protein ypjQ [Bacillus
UniRef100_P54173
Bacillus subtilis
YpjQ



subtilis]


2218



YpjP


2219



YpiP


2220
Hypothetical protein yphP [Bacillus
UniRef100_P54170
Bacillus subtilis
YphP



subtilis]


2221
Dihydroxy-acid dehydratase [Bacillus
UniRef100_P51785
Bacillus subtilis
IlvD



subtilis]


2222



YpgR


2223
Hypothetical protein ypgQ [Bacillus
UniRef100_P54168
Bacillus subtilis
YpgQ



subtilis]


2224
Glutathione peroxidase homolog bsaA
UniRef100_P52035
Bacillus subtilis
BsaA



[Bacillus subtilis]










2225
UPI00003CBA0F UniRef100 entry
UniRef100_UPI00003CBA0F












2226
Homoserine O-succinyltransferase
UniRef100_P54167
Bacillus subtilis
MetA



[Bacillus subtilis]


2227
Putative glycosyl transferase ypfP
UniRef100_P54166
Bacillus subtilis
UgtP



[Bacillus subtilis]


2228


2229
Cold shock protein cspD [Bacillus
UniRef100_P51777
Bacillus subtilis



subtilis]


2230
Regulatory protein degR [Bacillus
UniRef100_P06563
Bacillus subtilis



subtilis]


2231
Hypothetical protein ypzA [Bacillus
UniRef100_O32007
Bacillus subtilis



subtilis]


2232


2233
Hypothetical protein ypeP [Bacillus
UniRef100_P54164
Bacillus subtilis
YpeP



subtilis]


2234
Hypothetical protein ypdP [Bacillus
UniRef100_P54163
Bacillus subtilis
YpdP



subtilis]


2235
14.7 kDa ribonuclease H-like protein
UniRef100_P54162
Bacillus subtilis
YpdQ



[Bacillus subtilis]


2236
Probable 5′-3′ exonuclease [Bacillus
UniRef100_P54161
Bacillus subtilis
YpcP



subtilis]


2237


2238
Hypothetical protein ypbS [Bacillus
UniRef100_P54160
Bacillus subtilis



subtilis]


2239
Hypothetical protein ypbR [Bacillus
UniRef100_P54159
Bacillus subtilis
YpbR



subtilis]


2240


2241
Hypothetical protein ypbQ [Bacillus
UniRef100_P54158
Bacillus subtilis
YpbQ



subtilis]


2242



BcsA


2243
Predicted acetyltransferase
UniRef100_Q97G03
Clostridium
YokL



[Clostridium acetobutylicum]

acetobutylicum


2244
Xanthine permease [Bacillus subtilis]
UniRef100_P42086
Bacillus subtilis
PbuX


2245
Xanthine phosphoribosyltransferase
UniRef100_P42085
Bacillus subtilis
Xpt



[Bacillus subtilis]


2246
Hypothetical metalloprotease ypwA
UniRef100_P50848
Bacillus subtilis
YpwA



[Bacillus subtilis]


2247



YpvA


2248
Hypothetical protein yptA precursor
UniRef100_P50841
Bacillus subtilis



[Bacillus subtilis]


2249


2250
Hypothetical UPF0020 protein ypsC
UniRef100_P50840
Bacillus subtilis
YpsC



precursor [Bacillus subtilis]


2251
Hypothetical protein ypsB [Bacillus
UniRef100_P50839
Bacillus subtilis



subtilis]


2252
Hypothetical protein ypsA [Bacillus
UniRef100_P50838
Bacillus subtilis
YpsA



subtilis]


2253
Spore coat protein D [Bacillus subtilis]
UniRef100_P07791
Bacillus subtilis


2254


2255



YprB


2256



YprA


2257
Putative PTS system IIA component
UniRef100_P50829
Bacillus subtilis
YpqE



ypqE [Bacillus subtilis]


2258
Hypothetical protein ypqA precursor
UniRef100_P50836
Bacillus subtilis
YpqA



[Bacillus subtilis]


2259
Hypothetical protein yppG [Bacillus
UniRef100_P50835
Bacillus subtilis
YppG



subtilis]


2260


2261



YppE


2262


2263


2264
Hypothetical protein yppC [Bacillus
UniRef100_P39791
Bacillus subtilis
YppC



subtilis]


2265
Recombination protein U [Bacillus
UniRef100_P39792
Bacillus subtilis
RecU



subtilis]


2266
Penicillin-binding protein 1A/1B (PBP1)
UniRef100_P39793
Includes:
PonA



[Includes: Penicillin-insensitive

Penicillin-



transglycosylase (EC 2.4.2.—)

insensitive



(Peptidoglycan TGase); Penicillin-

transglycosylase



sensitive transpeptidase (EC 3.4.—.—)

(EC 2.4.2.—)



(DD-transpeptidase)] [Bacillus subtilis]

(Peptidoglycan





TGase);





Penicillin-





sensitive





transpeptidase





(EC 3.4.—.—) (DD-





transpeptidase)


2267
Hypothetical protein ypoC [Bacillus
UniRef100_P39789
Bacillus subtilis
YpoC



subtilis]


2268
Probable endonuclease III (EC
UniRef100_P39788
Bacillus subtilis
Nth



4.2.99.18) (DNA-(apurinic or



apyrimidinic site) lyase) [Bacillus



subtilis]


2269
DNA replication protein dnaD [Bacillus
UniRef100_P39787
Bacillus subtilis
DnaD



subtilis]


2270
Asparaginyl-tRNA synthetase [Bacillus
UniRef100_P39772
Bacillus subtilis
AsnS



subtilis]


2271
Aspartate aminotransferase [Bacillus
UniRef100_P53001
Bacillus subtilis
AspB



subtilis]


2272
Hypothetical protein ypmB [Bacillus
UniRef100_P54396
Bacillus subtilis
YpmB



subtilis]


2273
Hypothetical protein ypmA [Bacillus
UniRef100_P54395
Bacillus subtilis



subtilis]


2274
Probable ATP-dependent helicase
UniRef100_P54394
Bacillus subtilis
DinG



dinG homolog [Bacillus subtilis]


2275
Aspartate 1-decarboxylase precursor
UniRef100_P52999
Bacillus subtilis
PanD



[Bacillus subtilis]


2276
Pantoate--beta-alanine ligase [Bacillus
UniRef100_P52998
Bacillus subtilis
PanC



subtilis]


2277
3-methyl-2-oxobutanoate
UniRef100_P52996
Bacillus subtilis
PanB



hydroxymethyltransferase [Bacillus



subtilis]


2278
BirA bifunctional protein [Includes:
UniRef100_P42975
Includes: Biotin
BirA



Biotin operon repressor; Biotin--

operon



[acetyl-CoA-carboxylase] synthetase

repressor; Biotin--



(EC 6.3.4.15) (Biotin--protein ligase)]



[Bacillus subtilis]


2279
Poly(A) polymerase [Bacillus subtilis]
UniRef100_P42977
Bacillus subtilis
Cca


2280
Putative glycosyl transferase ypjH
UniRef100_P42982
Bacillus subtilis
YpjH



[Bacillus subtilis]


2281
Hypothetical protein ypjG [Bacillus
UniRef100_P42981
Bacillus subtilis
YpjG



subtilis]


2282
Methylglyoxal synthase [Bacillus
UniRef100_P42980
Bacillus subtilis
MgsA



subtilis]


2283
Dihydrodipicolinate reductase [Bacillus
UniRef100_P42976
Bacillus subtilis
DapB



subtilis]


2284
Hypothetical protein ypjD [Bacillus
UniRef100_P42979
Bacillus subtilis
YpjD



subtilis]


2285



YpjC


2286
Hypothetical protein ypjB precursor
UniRef100_P54393
Bacillus subtilis
YpjB



[Bacillus subtilis]


2287



YpjA


2288



QcrC


2289
Menaquinol-cytochrome c reductase
UniRef100_P46912
Bacillus subtilis
QcrB



cytochrome b subunit [Bacillus subtilis]


2290
Menaquinol-cytochrome c reductase
UniRef100_P46911
Bacillus subtilis
QcrA



iron-sulfur subunit [Bacillus subtilis]


2291
Hypothetical protein ypiF [Bacillus
UniRef100_P54391
Bacillus subtilis
YpiF



subtilis]


2292
Hypothetical UPF0302 protein ypiB
UniRef100_P54390
Bacillus subtilis
YpiB



[Bacillus subtilis]


2293
Hypothetical protein ypiA [Bacillus
UniRef100_P54389
Bacillus subtilis
YpiA



subtilis]


2294
3-phosphoshikimate 1-
UniRef100_P20691
Bacillus subtilis
AroE



carboxyvinyltransferase [Bacillus



subtilis]


2295
Prephenate dehydrogenase [Bacillus
UniRef100_P20692
Bacillus subtilis
TyrA



subtilis]


2296



HisC


2297
Tryptophan synthase alpha chain
UniRef100_P07601
Bacillus subtilis
TrpA



[Bacillus subtilis]


2298
Tryptophan synthase beta chain
UniRef100_P07600
Bacillus subtilis
TrpB



[Bacillus subtilis]


2299
N-(5′-phosphoribosyl)anthranilate
UniRef100_P20167
Bacillus subtilis
TrpF



isomerase [Bacillus subtilis]


2300
Indole-3-glycerol phosphate synthase
UniRef100_P03964
Bacillus subtilis
TrpC



[Bacillus subtilis]


2301
Anthranilate phosphoribosyltransferase
UniRef100_P03947
Bacillus subtilis
TrpD



[Bacillus subtilis]


2302
Anthranilate synthase component I
UniRef100_P03963
Bacillus subtilis
TrpE



[Bacillus subtilis]


2303
Chorismate mutase [Bacillus subtilis]
UniRef100_P19080
Bacillus subtilis
AroH


2304
3-dehydroquinate synthase [Bacillus
UniRef100_P31102
Bacillus subtilis
AroB



subtilis]


2305
Chorismate synthase [Bacillus subtilis]
UniRef100_P31104
Bacillus subtilis
AroF


2306
Chemotaxis protein methyltransferase
UniRef100_P31105
Bacillus subtilis
CheR



[Bacillus subtilis]


2307
Nucleoside diphosphate kinase
UniRef100_P31103
Bacillus subtilis
Ndk



[Bacillus subtilis]


2308
Heptaprenyl diphosphate synthase
UniRef100_P31114
Bacillus subtilis
HepT



component II [Bacillus subtilis]


2309
Menaquinone biosynthesis
UniRef100_P31113
Bacillus subtilis
MenH



methyltransferase ubiE [Bacillus



subtilis]


2310
Heptaprenyl diphosphate synthase
UniRef100_P31112
Bacillus subtilis
HepS



component I [Bacillus subtilis]


2311
Transcription attenuation protein mtrB
UniRef100_P19466
Bacillus subtilis



[Bacillus subtilis]


2312
GTP cyclohydrolase I [Bacillus subtilis]
UniRef100_P19465
Bacillus subtilis
MtrA


2313
DNA-binding protein HU 1 [Bacillus
UniRef100_P08821
Bacillus subtilis



subtilis]


2314
Stage IV sporulation protein A [Bacillus
UniRef100_P35149
Bacillus subtilis
SpoIVA



subtilis]


2315
Hypothetical protein yphF [Bacillus
UniRef100_P39911
Bacillus subtilis
YphF



subtilis]


2316
Hypothetical protein yphE [Bacillus
UniRef100_P50744
Bacillus subtilis



subtilis]


2317
Glycerol-3-phosphate dehydrogenase
UniRef100_P46919
NAD(P)+
GpsA



[NAD(P)+] (EC 1.1.1.94) (NAD(P)H-



dependent glycerol-3-phosphate



dehydrogenase) (NAD(P)H-dependent



dihydroxyacetone-phosphate



reductase) [Bacillus subtilis]


2318



YphC










2319
Hypothetical protein OB1798
UniRef100_Q8EQA7
Oceanobacillus iheyensis



[Oceanobacillus iheyensis]











2320
Hypothetical protein yphB [Bacillus
UniRef100_P50742
Bacillus subtilis
SeaA



subtilis]


2321
Hypothetical protein yphA [Bacillus
UniRef100_P50741
Bacillus subtilis
YphA



subtilis]


2322


2323



YpgA


2324
30S ribosomal protein S1 homolog
UniRef100_P38494
Bacillus subtilis
YpfD



[Bacillus subtilis]


2325
Cytidylate kinase [Bacillus subtilis]
UniRef100_P38493
Bacillus subtilis
Cmk


2326
Hypothetical protein ypfB [Bacillus
UniRef100_P38492
Bacillus subtilis



subtilis]


2327
Sporulation protein ypeB [Bacillus
UniRef100_P38490
Bacillus subtilis
YpeB



subtilis]


2328
Spore cortex-lytic enzyme precursor
UniRef100_P50739
Bacillus subtilis
SleB



[Bacillus subtilis]


2329



YpdC


2330



YccC


2331
Hypothetical protein ypdA [Bacillus
UniRef100_P50736
Bacillus subtilis
YpdA



subtilis]


2332
NAD-specific glutamate
UniRef100_P50735
Bacillus subtilis
GudB



dehydrogenase [Bacillus subtilis]


2333
Adapter protein mecA 2 [Bacillus
UniRef100_P50734
Bacillus subtilis
YpbH



subtilis]


2334
Hypothetical protein ypbG precursor
UniRef100_P50733
Bacillus subtilis
YpbG



[Bacillus subtilis]


2335



YpbF


2336
Hypothetical protein ypbE [Bacillus
UniRef100_P50731
Bacillus subtilis
YpbE



subtilis]


2337
Hypothetical protein ypbD [Bacillus
UniRef100_P50730
Bacillus subtilis
YpbD



subtilis]


2338
ATP-dependent DNA helicase recQ
UniRef100_P50729
Bacillus subtilis
RecQ



[Bacillus subtilis]


2339
Hypothetical protein ypbB [Bacillus
UniRef100_P50728
Bacillus subtilis
YpbB



subtilis]


2340
Ferredoxin [Bacillus subtilis]
UniRef100_P50727
Bacillus subtilis


2341
Hypothetical protein ypaA [Bacillus
UniRef100_P50726
Bacillus subtilis
YpaA



subtilis]


2342


2343
D-3-phosphoglycerate dehydrogenase
UniRef100_P35136
Bacillus subtilis
SerA



[Bacillus subtilis]










2344
BH1600 protein [Bacillus halodurans]
UniRef100_Q9KCH1
Bacillus halodurans











2345
Sigma-X negative effector [Bacillus
UniRef100_P35166
Bacillus subtilis
RsiX



subtilis]


2346
RNA polymerase sigma factor sigX
UniRef100_P35165
Bacillus subtilis
SigX



[Bacillus subtilis]


2347
Transcriptional regulator [Bacillus
UniRef100_Q72XJ3
Bacillus cereus
LytR



cereus]


2348
Endo-1,4-beta-xylanase [Bacillus
UniRef100_Q9K630
Bacillus
YheN



halodurans]

halodurans


2349


2350
Alkaline phosphatase synthesis sensor
UniRef100_Q898N3
Clostridium
YclK



protein phoR [Clostridium tetani]

tetani


2351
Response regulators consisting of a
UniRef100_Q8R9H7
Thermoanaerobacter
YycF



CheY-like receiver domain and a HTH

tengcongensis



DNA-binding domain



[Thermoanaerobacter tengcongensis]


2352
Sensor protein resE [Bacillus subtilis]
UniRef100_P35164
Bacillus subtilis
ResE


2353
Transcriptional regulatory protein resD
UniRef100_P35163
Bacillus subtilis
ResD



[Bacillus subtilis]


2354
Protein resC [Bacillus subtilis]
UniRef100_P35162
Bacillus subtilis
ResC


2355
ResB protein [Bacillus subtilis]
UniRef100_P35161
Bacillus subtilis
ResB


2356
Thiol-disulfide oxidoreductase resA
UniRef100_P35160
Bacillus subtilis
ResA



[Bacillus subtilis]


2357
Ribosomal large subunit pseudouridine
UniRef100_P35159
Bacillus subtilis
RluB



synthase B [Bacillus subtilis]


2358
Spore maturation protein B [Bacillus
UniRef100_P35158
Bacillus subtilis
SpmB



subtilis]


2359
Spore maturation protein A [Bacillus
UniRef100_P35157
Bacillus subtilis
SpmA



subtilis]


2360
Penicillin-binding protein 5* precursor
UniRef100_P35150
Bacillus subtilis
DacB



[Bacillus subtilis]


2361
Hypothetical protein ypul [Bacillus
UniRef100_P35156
Bacillus subtilis
YpuI



subtilis]


2362
Segregation and condensation protein
UniRef100_P35155
Bacillus subtilis
YpuH



B [Bacillus subtilis]


2363
Segregation and condensation protein
UniRef100_P35154
Bacillus subtilis
YpuG



A [Bacillus subtilis]


2364


2365
Hypothetical protein ypuF [Bacillus
UniRef100_P17617
Bacillus subtilis
YpuF



subtilis]


2366
RibT protein [Bacillus subtilis]
UniRef100_P17622
Bacillus subtilis
RibT


2367
6,7-dimethyl-8-ribityllumazine synthase
UniRef100_Q44681
Bacillus
RibH



[Bacillus amyloliquefaciens]

amyloliquefaciens


2368
Riboflavin biosynthesis protein ribA
UniRef100_P17620
Includes: GTP
RibA



[Includes: GTP cyclohydrolase II (EC

cyclohydrolase II



3.5.4.25); 3,4-dihydroxy-2-butanone 4-

(EC 3.5.4.25);



phosphate synthase (DHBP synthase)]

3,4-dihydroxy-2-



[Bacillus subtilis]

butanone 4-





phosphate





synthase (DHBP





synthase)


2369
Riboflavin synthase alpha chain
UniRef100_P16440
Bacillus subtilis
RibE



[Bacillus subtilis]


2370
Riboflavin biosynthesis protein ribD
UniRef100_P17618
Includes:
RibD



[Includes:

Diaminohydroxyphosphoribosylaminopyrimidine



Diaminohydroxyphosphoribosylaminopyrimidine

deaminase (EC



deaminase (EC 3.5.4.26)

3.5.4.26)



(Riboflavin-specific deaminase); 5-

(Riboflavin-



amino-6-(5-

specific



phosphoribosylamino)uracil reductase

deaminase); 5-



(EC 1.1.1.193) (HTP reductase)]

amino-6-(5-



[Bacillus subtilis]

phosphoribosylamino)





uracil





reductase (EC





1.1.1.193) (HTP





reductase)


2371
Hypothetical protein ypuD [Bacillus
UniRef100_P17616
Bacillus subtilis
YpuD



subtilis]


2372
Putative serine/threonine protein
UniRef100_Q7VFC1
Helicobacter
YjbP



phosphatase [Helicobacter hepaticus]

hepaticus


2373
Stress response homolog Hsp [Bacillus
UniRef100_Q9X3Z5
Bacillus subtilis



subtilis]


2374
Response regulator aspartate
UniRef100_Q00828
Bacillus subtilis
RapA



phosphatase A [Bacillus subtilis]


2375
Peptidyl-prolyl cis-trans isomerase B
UniRef100_P35137
Bacillus subtilis
PpiB



[Bacillus subtilis]


2376



YpuA










2377
IS1627s1-related, transposase
UniRef100_Q7CMD0
Bacillus anthracis str. A2012



[Bacillus anthracis str. A2012]










2378
UPI00003CC069 UniRef100 entry
UniRef100_UPI00003CC069












2379



YndL


2380
Diaminopimelate decarboxylase
UniRef100_P23630
Bacillus subtilis
LysA



[Bacillus subtilis]


2381


2382
Stage V sporulation protein AF
UniRef100_P31845
Bacillus subtilis
SpoVAF



[Bacillus subtilis]


2383
Stage V sporulation protein AE
UniRef100_P40870
Bacillus subtilis
SpoVAE



[Bacillus subtilis]


2384
Stage V sporulation protein AE
UniRef100_P40870
Bacillus subtilis
SpoVAE



[Bacillus subtilis]


2385
Stage V sporulation protein AD
UniRef100_P40869
Bacillus subtilis
SpoVAD



[Bacillus subtilis]


2386



SpoVAC


2387
Stage V sporulation protein AB
UniRef100_P40867
Bacillus subtilis
SpoVAB



[Bacillus subtilis]


2388
Stage V sporulation protein AA
UniRef100_P40866
Bacillus subtilis
SpoVAA



[Bacillus subtilis]


2389



SigF


2390



SpoIIAB


2391



SpoIIAA


2392
Penicillin-binding protein dacF
UniRef100_P38422
Bacillus subtilis
DacF



precursor [Bacillus subtilis]


2393
Purine nucleoside phosphorylase I
UniRef100_P46354
Bacillus subtilis
PunA



[Bacillus subtilis]


2394
Phosphopentomutase [Bacillus subtilis]
UniRef100_P46353
Bacillus subtilis
Drm


2395
Tyrosine recombinase xerD [Bacillus
UniRef100_P46352
Bacillus subtilis
RipX



subtilis]


2396


2397
Ferric uptake regulation protein
UniRef100_P54574
Bacillus subtilis
Fur



[Bacillus subtilis]


2398
Stage II sporulation protein M [Bacillus
UniRef100_P37873
Bacillus subtilis
SpoIIM



subtilis]


2399
Lmo2763 protein [Listeria
UniRef100_Q926Y4
Listeria
YdhO



monocytogenes]

monocytogenes










2400
UPI00003CA2F1 UniRef100 entry
UniRef100_UPI00003CA2F1












2401
Probable allantoin permease [Bacillus
UniRef100_P94575
Bacillus subtilis
YwoE



subtilis]


2402



YdaF


2403
Hypothetical protein yqkK [Bacillus
UniRef100_P54573
Bacillus subtilis



subtilis]


2404



YybD


2405
Hypothetical protein [Bacillus pumilus]
UniRef100_Q93PN4
Bacillus pumilus
YqxK


2406
ADP-ribose pyrophosphatase [Bacillus
UniRef100_P54570
Bacillus subtilis
NudE



subtilis]


2407


2408
YdgC protein [Bacillus subtilis]
UniRef100_P96701
Bacillus subtilis
YdgC


2409
Hypothetical protein ydgD [Bacillus
UniRef100_P96702
Bacillus subtilis
YdgD



subtilis]


2410
Hypothetical oxidoreductase yqkF
UniRef100_P54569
Bacillus subtilis
YqkF



[Bacillus subtilis]


2411


2412



YqkD


2413
Hypothetical protein yqkC [Bacillus
UniRef100_P54566
Bacillus subtilis



subtilis]


2414
Hypothetical protein yqkB [Bacillus
UniRef100_P54565
Bacillus subtilis
YqkB



subtilis]










2415
UPI00003CBE2B UniRef100 entry
UniRef100_UPI00003CBE2B












2416
Hypothetical UPF0157 protein yqkA
UniRef100_P54564
Bacillus subtilis
YqkA



[Bacillus subtilis]


2417
Hypothetical protein yqjZ [Bacillus
UniRef100_P54563
Bacillus subtilis
YqjZ



subtilis]


2418
Lipase precursor [Bacillus subtilis]
UniRef100_P37957
Bacillus subtilis
Lip


2419


2420
Nickel ABC transporter [Bacillus
UniRef100_Q9KBX8
Bacillus
AppA



halodurans]

halodurans


2421
Nickel ABC transporter [Bacillus
UniRef100_Q9KBX7
Bacillus
AppB



halodurans]

halodurans


2422
Nickel ABC transporter [Bacillus
UniRef100_Q9KBX6
Bacillus
AppC



halodurans]

halodurans


2423
Putative oligopeptide ABC transporter
UniRef100_Q895A4
Clostridium
DppD



[Clostridium tetani]

tetani


2424
Putative oligopeptide ABC transporter
UniRef100_Q895A5
Clostridium
OppF



[Clostridium tetani]

tetani


2425
DNA-damage-inducible protein
UniRef100_Q9KDR1
Bacillus
YojI



[Bacillus halodurans]

halodurans










2426
Fibronectin-binding protein [Bacillus
UniRef100_Q63CW1
Bacillus cereus ZK



cereus ZK]











2427
Hypothetical protein yolD
UniRef100_O64030
Bacteriophage
YolD



[Bacteriophage SPBc2]

SPBc2


2428



UvrX


2429
Hypothetical protein yqzH [Bacillus
UniRef100_O32014
Bacillus subtilis



subtilis]


2430
Hypothetical transport protein yqjV
UniRef100_P54559
Bacillus subtilis
YqjV



[Bacillus subtilis]


2431
Hypothetical protein yqjT [Bacillus
UniRef100_P54557
Bacillus subtilis
YqjT



subtilis]


2432



CoaA










2433
LacG [Lactococcus lactis]
UniRef100_Q9RAU9
Lactococcus lactis











2434
LacF [Lactococcus lactis]
UniRef100_Q9RAV2
Lactococcus
YdbJ





lactis


2435










2436
Hypothetical protein [Bacillus
UniRef100_Q70K07
Bacillus amyloliquefaciens



amyloliquefaciens]











2437
Hypothetical protein [Bacillus cereus]
UniRef100_Q737G3
Bacillus cereus



2438
YmaC protein [Bacillus subtilis]
UniRef100_O31789
Bacillus subtilis
YmaC


2439
Hypothetical oxidoreductase yqjQ
UniRef100_P54554
Bacillus subtilis
YqjQ



[Bacillus subtilis]


2440



YqjP


2441



ProI


2442
Hypothetical protein yqjN [Bacillus
UniRef100_P54551
Bacillus subtilis
YqjN



subtilis]


2443
Probable NADH-dependent flavin
UniRef100_P54550
Bacillus subtilis
YqjM



oxidoreductase yqjM [Bacillus subtilis]


2444
Hypothetical protein yqjL [Bacillus
UniRef100_P54549
Bacillus subtilis
YqjL



subtilis]


2445


2446
Ribonuclease Z [Bacillus subtilis]
UniRef100_P54548
Bacillus subtilis
YqjK


2447
Glucose-6-phosphate 1-
UniRef100_P54547
Bacillus subtilis
Zwf



dehydrogenase [Bacillus subtilis]


2448
Hypothetical conserved protein
UniRef100_Q8ELZ9
Oceanobacillus
CitH



[Oceanobacillus iheyensis]

iheyensis










2449
Hypothetical protein OB3065
UniRef100_Q8ELZ8
Oceanobacillus iheyensis



[Oceanobacillus iheyensis]











2450
Immunogenic protein [Oceanobacillus
UniRef100_Q8ELZ7
Oceanobacillus
SsuA



iheyensis]

iheyensis


2451
YdhQ protein [Bacillus subtilis]
UniRef100_O05509
Bacillus subtilis
YdhQ


2452
Beta-glucosidase [Bacillus halodurans]
UniRef100_Q9K615
Bacillus
YdhP





halodurans


2453
Putative cellobiose-specific enzyme IIC
UniRef100_Q8KP28
Bacillus pumilus
YdhO



[Bacillus pumilus]


2454
YdhO protein [Bacillus subtilis]
UniRef100_O05507
Bacillus subtilis
YdhO


2455
YdhN protein [Bacillus subtilis]
UniRef100_O05506
Bacillus subtilis
YdhN










2456
PTS system, cellobiose-specific
UniRef100_Q9K613
Bacillus halodurans



enzyme II, B component [Bacillus



halodurans]











2457
Alkaline phosphatase [Bacillus cereus
UniRef100_Q639W1
Bacillus cereus
PhoB



ZK]

ZK


2458
Glucose 1-dehydrogenase A [Bacillus
UniRef100_P10528
Bacillus
Gdh



megaterium]

megaterium


2459
6-phosphogluconate dehydrogenase,
UniRef100_P80859
Bacillus subtilis
YqjI



decarboxylating II [Bacillus subtilis]


2460
DNA polymerase IV 1 [Bacillus subtilis]
UniRef100_P54545
Bacillus subtilis
YqjH


2461
Hypothetical protein yqzJ [Bacillus
UniRef100_Q7WY64
Bacillus subtilis



subtilis]


2462



YqjG


2463
Hypothetical protein yqjE [Bacillus
UniRef100_P54542
Bacillus subtilis
YqjE



subtilis]


2464
Methylmalonyl-CoA decarboxylase
UniRef100_Q9K8P6
Bacillus
YqjD



alpha subunit [Bacillus halodurans]

halodurans


2465
Hypothetical protein yqjA [Bacillus
UniRef100_P54538
Bacillus subtilis
YqjA



subtilis]


2466
Probable amino-acid ABC transporter
UniRef100_P54537
Bacillus subtilis
YqiZ



ATP-binding protein yqiZ [Bacillus



subtilis]


2467
Probable amino-acid ABC transporter
UniRef100_P54536
Bacillus subtilis
YqiY



permease protein yqiY [Bacillus



subtilis]


2468
Probable amino-acid ABC transporter
UniRef100_P54535
Bacillus subtilis
YqiX



extracellular binding protein yqiX



precursor [Bacillus subtilis]


2469
Hypothetical protein yqiW [Bacillus
UniRef100_P54534
Bacillus subtilis
YqiW



subtilis]


2470
Protein bmrU [Bacillus subtilis]
UniRef100_P39074
Bacillus subtilis
BmrU


2471
Lipoamide acyltransferase component
UniRef100_P37942
Bacillus subtilis
BkdB



of branched-chain alpha-keto acid



dehydrogenase complex (EC



2.3.1.168) (Dihydrolipoyllysine-residue



(2-methylpropanoyl)transferase)



[Bacillus subtilis]


2472
2-oxoisovalerate dehydrogenase beta
UniRef100_P37941
Bacillus subtilis
BkdAB



subunit [Bacillus subtilis]


2473
2-oxoisovalerate dehydrogenase alpha
UniRef100_P37940
Bacillus subtilis
BkdAA



subunit [Bacillus subtilis]


2474
Dihydrolipoyl dehydrogenase [Bacillus
UniRef100_P54533
Bacillus subtilis
LpdV



subtilis]


2475
Probable butyrate kinase [Bacillus
UniRef100_P54532
Bacillus subtilis
Buk



subtilis]


2476
Leucine dehydrogenase [Bacillus
UniRef100_P54531
Bacillus subtilis
Bcd



subtilis]


2477
Probable phosphate butyryltransferase
UniRef100_P54530
Bacillus subtilis
Ptb



[Bacillus subtilis]


2478
Putative sigma L-dependent
UniRef100_P54529
Bacillus subtilis
BkdR



transcriptional regulator yqiR [Bacillus



subtilis]


2479
Hypothetical protein yqzF [Bacillus
UniRef100_O32015
Bacillus subtilis



subtilis]


2480
Hypothetical protein yqiK [Bacillus
UniRef100_P54527
Bacillus subtilis
YqiK



subtilis]


2481
Hypothetical protein yqiI [Bacillus
UniRef100_P54525
Bacillus subtilis
YqiI



subtilis]


2482



YqiH


2483
Serine O-acetyltransferase
UniRef100_Q8PSY4
Methanosarcina
CysE



[Methanosarcina mazei]

mazei


2484


2485
Stage 0 sporulation protein A [Bacillus
UniRef100_P06534
Bacillus subtilis
Spo0A



subtilis]


2486



SpoIVB


2487
DNA repair protein recN [Bacillus
UniRef100_Q659H4
Bacillus
RecN



amyloliquefaciens]

amyloliquefaciens


2488
Arginine repressor [Bacillus subtilis]
UniRef100_P17893
Bacillus subtilis
AhrC


2489
Hypothetical protein yqxC [Bacillus
UniRef100_P19672
Bacillus subtilis
YqxC



subtilis]


2490



Dxs


2491
Geranyltranstransferase [Bacillus
UniRef100_P54383
Bacillus subtilis
YqiD



subtilis]


2492
Probable exodeoxyribonuclease VII
UniRef100_P54522
Bacillus subtilis



small subunit [Bacillus subtilis]


2493
Probable exodeoxyribonuclease VII
UniRef100_P54521
Bacillus subtilis
YqiB



large subunit [Bacillus subtilis]


2494
FolD bifunctional protein [Includes:
UniRef100_P54382
Includes:
FolD



Methylenetetrahydrofolate

Methylenetetrahydrofolate



dehydrogenase (EC 1.5.1.5);

dehydrogenase



Methenyltetrahydrofolate

(EC 1.5.1.5);



cyclohydrolase (EC 3.5.4.9)] [Bacillus

Methenyltetrahydrofolate



subtilis]

cyclohydrolase





(EC 3.5.4.9)


2495
N utilization substance protein B
UniRef100_P54520
Bacillus subtilis
NusB



homolog [Bacillus subtilis]


2496
Hypothetical protein yqhY [Bacillus
UniRef100_P54519
Bacillus subtilis
YqhY



subtilis]


2497



AccC


2498
Biotin carboxyl carrier protein of acetyl-
UniRef100_P49786
Bacillus subtilis
AccB



CoA carboxylase [Bacillus subtilis]


2499
Stage III sporulation protein AH
UniRef100_P49785
Bacillus subtilis
SpoIIIAH



[Bacillus subtilis]


2500
Stage III sporulation protein AG
UniRef100_P49784
Bacillus subtilis
SpoIIIAG



[Bacillus subtilis]


2501
Stage III sporulation protein AF
UniRef100_P49783
Bacillus subtilis
SpoIIIAF



[Bacillus subtilis]


2502
Stage III sporulation protein AE
UniRef100_P49782
Bacillus subtilis
SpoIIIAE



[Bacillus subtilis]


2503
Stage III sporulation protein AD
UniRef100_P49781
Bacillus subtilis
SpoIIIAD



[Bacillus subtilis]


2504
Stage III sporulation protein AC
UniRef100_P49780
Bacillus subtilis



[Bacillus subtilis]


2505
Stage III sporulation protein AB
UniRef100_Q01368
Bacillus subtilis
SpoIIIAB



[Bacillus subtilis]


2506



SpoIIIAA


2507
Hypothetical protein yqhV [Bacillus
UniRef100_P49779
Bacillus subtilis



subtilis]


2508
Elongation factor P [Bacillus subtilis]
UniRef100_P49778
Bacillus subtilis
Efp


2509
Putative peptidase yqhT [Bacillus
UniRef100_P54518
Bacillus subtilis
YqhT



subtilis]


2510
3-dehydroquinate dehydratase
UniRef100_P54517
Bacillus subtilis
YqhS



[Bacillus subtilis]


2511
Hypothetical protein yqhR [Bacillus
UniRef100_P54516
Bacillus subtilis
YqhR



subtilis]


2512
Hypothetical protein yqhQ [Bacillus
UniRef100_P54515
Bacillus subtilis
YqhQ



subtilis]


2513
Hypothetical protein yqhP [Bacillus
UniRef100_P54514
Bacillus subtilis
YqhP



subtilis]


2514
Hypothetical protein yqhO [Bacillus
UniRef100_P54513
Bacillus subtilis
YqhO



subtilis]


2515
Transcriptional regulator mntR [Bacillus
UniRef100_P54512
Bacillus subtilis
MntR



subtilis]


2516
Hypothetical protein yqhM [Bacillus
UniRef100_P54511
Bacillus subtilis
YqhM



subtilis]


2517
Hypothetical protein yqhL [Bacillus
UniRef100_P54510
Bacillus subtilis
YqhL



subtilis]


2518
Glycine betaine-binding protein
UniRef100_P46922
Bacillus subtilis
OpuAC



precursor [Bacillus subtilis]


2519
Glycine betaine transport system
UniRef100_P46921
Bacillus subtilis
OpuAB



permease protein opuAB [Bacillus



subtilis]


2520
Glycine betaine transport ATP-binding
UniRef100_P46920
Bacillus subtilis
OpuAA



protein opuAA [Bacillus subtilis]


2521
Probable glycine dehydrogenase
UniRef100_P54377
decarboxylating
GcvPB



[decarboxylating] subunit 2 [Bacillus



subtilis]


2522
Probable glycine dehydrogenase
UniRef100_P54376
decarboxylating
GcvPA



[decarboxylating] subunit 1 [Bacillus



subtilis]


2523
Aminomethyltransferase [Bacillus
UniRef100_P54378
Bacillus subtilis
GcvT



subtilis]


2524
Hypothetical helicase yqhH [Bacillus
UniRef100_P54509
Bacillus subtilis
YqhH



subtilis]


2525
Hypothetical protein yqhG [Bacillus
UniRef100_P54508
Bacillus subtilis
YqhG



subtilis]


2526


2527



SinR


2528
Spore coat-associated protein N
UniRef100_P54507
Bacillus subtilis
TasA



[Bacillus subtilis]


2529
Signal peptidase I W [Bacillus subtilis]
UniRef100_P54506
Bacillus subtilis
SipW


2530
Hypothetical protein yqxM [Bacillus
UniRef100_P40949
Bacillus subtilis
YqxM



subtilis]


2531
YqzG protein [Bacillus subtilis]
UniRef100_O32019
Bacillus subtilis
YqzG


2532
YqzE protein [Bacillus subtilis]
UniRef100_O32020
Bacillus subtilis


2533



ComGG


2534



ComGF


2535
ComG operon protein 5 precursor
UniRef100_P25957
Bacillus subtilis
ComGE



[Bacillus subtilis]


2536
ComG operon protein 4 precursor
UniRef100_P25956
Bacillus subtilis
ComGD



[Bacillus subtilis]


2537
ComG operon protein 3 precursor
UniRef100_P25955
Bacillus subtilis



[Bacillus subtilis]


2538



ComGB


2539
ComG operon protein 1 [Bacillus
UniRef100_P25953
Bacillus subtilis
ComGA



subtilis]


2540
Hypothetical protein yqhA [Bacillus
UniRef100_P54504
Bacillus subtilis
YqhA



subtilis]


2541
Hypothetical protein yqgZ [Bacillus
UniRef100_P54503
Bacillus subtilis
YqgZ



subtilis]


2542
Hypothetical protein yqgY [Bacillus
UniRef100_P54502
Bacillus subtilis



subtilis]


2543
Hypothetical protein yqgX [Bacillus
UniRef100_P54501
Bacillus subtilis
YqgX



subtilis]


2544
Hypothetical protein yqgW [Bacillus
UniRef100_P54500
Bacillus subtilis



subtilis]










2545
Hypothetical protein [Staphylococcus
UniRef100_Q8CSE8
Staphylococcus epidermidis



epidermidis]











2546



YqgU


2547
Hypothetical protein yqgT [Bacillus
UniRef100_P54497
Bacillus subtilis
YqgT



subtilis]


2548
Ferrichrome-binding protein precursor
UniRef100_P37580
Bacillus subtilis
FhuD



[Bacillus subtilis]


2549
Hypothetical protein yqgS [Bacillus
UniRef100_P54496
Bacillus subtilis
YqgS



subtilis]


2550
Glucokinase [Bacillus subtilis]
UniRef100_P54495
Bacillus subtilis
GlcK


2551
Hypothetical protein yqgQ [Bacillus
UniRef100_P54494
Bacillus subtilis



subtilis]


2552
Stage V sporulation protein AF
UniRef100_Q8EQ08
Oceanobacillus
SpoVAF



[Oceanobacillus iheyensis]

iheyensis


2553
Hypothetical protein yqgP [Bacillus
UniRef100_P54493
Bacillus subtilis
YqgP



subtilis]


2554
Hypothetical protein yqgO [Bacillus
UniRef100_P54492
Bacillus subtilis



subtilis]


2555
Hypothetical protein yqgN [Bacillus
UniRef100_P54491
Bacillus subtilis
YqgN



subtilis]


2556


2557



YqgM


2558
Hypothetical protein yqgL [Bacillus
UniRef100_P54489
Bacillus subtilis
YqgL



subtilis]


2559



YqzD


2560
YqzC protein [Bacillus subtilis]
UniRef100_O32023
Bacillus subtilis
YqzC


2561
Phosphate import ATP-binding protein
UniRef100_P46342
Bacillus subtilis
PstBB



pstB 1 [Bacillus subtilis]


2562
Phosphate import ATP-binding protein
UniRef100_P46341
Bacillus subtilis
PstBA



pstB 2 [Bacillus subtilis]


2563
Probable ABC transporter permease
UniRef100_P46340
Bacillus subtilis
PstA



protein yqqI [Bacillus subtilis]


2564
Probable ABC transporter permease
UniRef100_P46339
Bacillus subtilis
PstC



protein yqgH [Bacillus subtilis]


2565
Probable ABC transporter binding
UniRef100_P46338
Bacillus subtilis
PstS



protein yqgG precursor [Bacillus



subtilis]


2566
Hypothetical protein yqgF [Bacillus
UniRef100_P54488
Bacillus subtilis
PbpA



subtilis]


2567
Hypothetical protein yqgE [Bacillus
UniRef100_P54487
Bacillus subtilis
YqgE



subtilis]


2568



SodA


2569
Hypothetical protein yqgC [Bacillus
UniRef100_P54486
Bacillus subtilis
YqgC



subtilis]


2570
Hypothetical protein yqgB [Bacillus
UniRef100_P54485
Bacillus subtilis
YqgB



subtilis]


2571
Hypothetical protein yqfZ [Bacillus
UniRef100_P54483
Bacillus subtilis



subtilis]


2572
4-hydroxy-3-methylbut-2-en-1-yl
UniRef100_P54482
Bacillus subtilis
YqfY



diphosphate synthase (EC 1.17.4.3) (1-



hydroxy-2-methyl-2-(E)-butenyl 4-



diphosphate synthase) [Bacillus



subtilis]


2573
Hypothetical protein yqfX [Bacillus
UniRef100_P54481
Bacillus subtilis
YqfX



subtilis]


2574
Putative nucleotidase yqfW [Bacillus
UniRef100_P54480
Bacillus subtilis
YqfW



subtilis]


2575
Zinc-specific metalloregulatory protein
UniRef100_P54479
Bacillus subtilis
Zur



[Bacillus subtilis]


2576
Metal (Zinc) transport protein [Listeria
UniRef100_Q926D9
Listeria innocua
YceA



innocua]


2577



YcdI


2578
Hypothetical protein yqfU [Bacillus
UniRef100_P54478
Bacillus subtilis
YqfU



subtilis]


2579


2580
Probable endonuclease IV [Bacillus
UniRef100_P54476
Bacillus subtilis
YqfS



subtilis]


2581
Probable RNA helicase yqfR [Bacillus
UniRef100_P54475
Bacillus subtilis
YqfR



subtilis]


2582
Hypothetical protein yqfQ [Bacillus
UniRef100_P54474
Bacillus subtilis
YqfQ



subtilis]


2583
4-hydroxy-3-methylbut-2-enyl
UniRef100_P54473
Bacillus subtilis
YqfP



diphosphate reductase [Bacillus



subtilis]


2584



YqfO


2585
Hypothetical protein yqfN [Bacillus
UniRef100_P54471
Bacillus subtilis
YqfN



subtilis]


2586
YwqL protein [Bacillus subtilis]
UniRef100_P96724
Bacillus subtilis
YwqL


2587


2588


2589


2590










2591
Hypothetical protein CAC0336
UniRef100_Q97M62
Clostridium acetobutylicum



[Clostridium acetobutylicum]


2592
Hypothetical protein [Bacillus
UniRef100_Q6HGF2
Bacillus thuringiensis



thuringiensis]











2593






2594
YwqJ protein [Bacillus subtilis]
UniRef100_P96722
Bacillus subtilis
YwqJ


2595
Hypothetical protein ywqI [Bacillus
UniRef100_P96721
Bacillus subtilis



subtilis]


2596



YwqH


2597
Cytochrome c-550 [Bacillus subtilis]
UniRef100_P24469
Bacillus subtilis
CccA


2598



SigA


2599
DNA primase [Bacillus subtilis]
UniRef100_P05096
Bacillus subtilis
DnaG


2600
Hypothetical UPF0178 protein yqxD
UniRef100_P17868
Bacillus subtilis
YqxD



[Bacillus subtilis]


2601
Hypothetical UPF0085 protein yqfL
UniRef100_P54470
Bacillus subtilis
YqfL



[Bacillus subtilis]


2602
YqzB protein [Bacillus subtilis]
UniRef100_O34994
Bacillus subtilis
YqzB


2603
Glycyl-tRNA synthetase beta chain
UniRef100_P54381
Bacillus subtilis
GlyS



[Bacillus subtilis]


2604
Glycyl-tRNA synthetase alpha chain
UniRef100_P54380
Bacillus subtilis
GlyQ



[Bacillus subtilis]


2605
DNA repair protein recO [Bacillus
UniRef100_P42095
Bacillus subtilis
RecO



subtilis]


2606


2607
GTP-binding protein era homolog
UniRef100_P42182
Bacillus subtilis
Era



[Bacillus subtilis]


2608
Cytidine deaminase [Bacillus subtilis]
UniRef100_P19079
Bacillus subtilis
Cdd


2609


2610
Hypothetical UPF0054 protein yqfG
UniRef100_P46347
Bacillus subtilis
YqfG



[Bacillus subtilis]


2611
Hypothetical protein yqfF [Bacillus
UniRef100_P46344
Bacillus subtilis
YqfF



subtilis]


2612
PhoH-like protein [Bacillus subtilis]
UniRef100_P46343
Bacillus subtilis
PhoH


2613



YqfD


2614
Hypothetical protein yqfC [Bacillus
UniRef100_P54468
Bacillus subtilis



subtilis]


2615
Hypothetical protein yqfB [Bacillus
UniRef100_P54467
Bacillus subtilis
YqfB



subtilis]


2616
Hypothetical protein yqfA [Bacillus
UniRef100_P54466
Bacillus subtilis
YqfA



subtilis]


2617



YqeZ


2618
Hypothetical protein yqeY [Bacillus
UniRef100_P54464
Bacillus subtilis
YqeY



subtilis]


2619


2620
Hypothetical protein yqeW [Bacillus
UniRef100_P54463
Bacillus subtilis
YqeW



subtilis]


2621
Deoxyribose-phosphate aldolase
UniRef100_Q92A19
Listeria innocua
Dra



[Listeria innocua]


2622
Hypothetical UPF0004 protein yqeV
UniRef100_P54462
Bacillus subtilis
YqeV



[Bacillus subtilis]


2623
Hypothetical UPF0088 protein yqeU
UniRef100_P54461
Bacillus subtilis
YqeU



[Bacillus subtilis]


2624
Ribosomal protein L11
UniRef100_P54460
Bacillus subtilis
YqeT



methyltransferase [Bacillus subtilis]


2625
Chaperone protein dnaJ [Bacillus
UniRef100_P17631
Bacillus subtilis
DnaJ



subtilis]


2626



DnaK


2627


2628


2629
Heat-inducible transcription repressor
UniRef100_P25499
Bacillus subtilis
HrcA



hrcA [Bacillus subtilis]


2630
Probable oxygen-independent
UniRef100_P54304
Bacillus subtilis
HemN



coproporphyrinogen III oxidase



[Bacillus subtilis]


2631



LepA


2632
Hypothetical protein yqxA [Bacillus
UniRef100_P38425
Bacillus subtilis
YqxA



subtilis]


2633



SpoIIP


2634
Germination protease precursor
UniRef100_P22322
Bacillus subtilis
Gpr



[Bacillus subtilis]


2635
30S ribosomal protein S20 [Bacillus
UniRef100_P21477
Bacillus subtilis



subtilis]


2636
Hypothetical protein yqeN [Bacillus
UniRef100_P54459
Bacillus subtilis
YqeN



subtilis]


2637


2638
ComE operon protein 3 [Bacillus
UniRef100_P39695
Bacillus subtilis
ComEC



subtilis]


2639
ComE operon protein 2 [Bacillus
UniRef100_P32393
Bacillus subtilis
ComEB



subtilis]


2640
ComE operon protein 1 [Bacillus
UniRef100_P39694
Bacillus subtilis
ComEA



subtilis]


2641
ComE operon protein 4 [Bacillus
UniRef100_P39696
Bacillus subtilis
ComER



subtilis]


2642
Hypothetical protein yqeM [Bacillus
UniRef100_P54458
Bacillus subtilis
YqeM



subtilis]


2643
Hypothetical protein yqeL [Bacillus
UniRef100_P54457
Bacillus subtilis
YqeL



subtilis]


2644
Hypothetical protein yqeK [Bacillus
UniRef100_P54456
Bacillus subtilis
YqeK



subtilis]


2645
Nicotinate-nucleotide
UniRef100_P54455
Bacillus subtilis
YqeJ



adenylyltransferase (EC 2.7.7.18)



(Deamido-NAD(+) pyrophosphorylase)



(Deamido-NAD(+) diphosphorylase)



[Bacillus subtilis]


2646
Hypothetical UPF0044 protein yqeI
UniRef100_P54454
Bacillus subtilis



[Bacillus subtilis]


2647
Shikimate dehydrogenase [Bacillus
UniRef100_P54374
Bacillus subtilis
AroD



subtilis]


2648
Hypothetical protein yqeH [Bacillus
UniRef100_P54453
Bacillus subtilis
YqeH



subtilis]


2649
Hypothetical protein yqeG [Bacillus
UniRef100_P54452
Bacillus subtilis
YqeG



subtilis]


2650


2651


2652
Hypothetical lipoprotein yqeF precursor
UniRef100_P54451
Bacillus subtilis
YqeF



[Bacillus subtilis]


2653
Acetyltransferase, GNAT family
UniRef100_Q81KW8
Bacillus
YdfB



[Bacillus anthracis]

anthracis


2654
Hypothetical protein yrhF [Bacillus
UniRef100_O05398
Bacillus subtilis
YrhF



subtilis]


2655
Formate dehydrogenase chain A
UniRef100_O05397
Bacillus subtilis
YrhE



[Bacillus subtilis]


2656
Hypothetical protein yrhD [Bacillus
UniRef100_O05396
Bacillus subtilis
YrhD



subtilis]


2657


2658
RNA polymerase sigma-K factor
UniRef100_P12254
Bacillus subtilis
SpoIIIC



precursor [Bacillus subtilis]


2659



YcnB


2660
BH2157 protein [Bacillus halodurans]
UniRef100_Q9KAX9
Bacillus
YuaI





halodurans


2661


2662


2663
Alanyl-tRNA synthetase family protein
UniRef100_Q81Y73
Bacillus
AlaS



[Bacillus anthracis]

anthracis


2664
METAL-ACTIVATED PYRIDOXAL
UniRef100_Q8YCI2
Brucella melitensis



ENZYME [Brucella melitensis]


2665
Probable translation initiation inhibitor
UniRef100_Q6LKM3
Photobacterium
YabJ



[Photobacterium profundum)]

profundum)


2666



YccC


2667
Putative threonine synthase
UniRef100_Q82IF6
Streptomyces
ThrC



[Streptomyces avermitilis]

avermitilis


2668



YabJ










2669
Hypothetical protein
UniRef100_Q8RBA0
Thermoanaerobacter



[Thermoanaerobacter tengcongensis]

tengcongensis








2670











2671
UPI000032CE59 UniRef100 entry
UniRef100_UPI000032CE59












2672
Multidrug-efflux transporter 1 regulator
UniRef100_P39075
Bacillus subtilis
BmrR



[Bacillus subtilis]


2673
Metallo-beta-lactamase/rhodanese-like
UniRef100_Q81Q95
Bacillus
YrkH



domain protein [Bacillus anthracis]

anthracis


2674
Hypothetical protein [Bacillus cereus
UniRef100_Q63B51
Bacillus cereus
YumB



ZK]

ZK


2675
NreC [Staphylococcus carnosus]
UniRef100_Q7WZY4
Staphylococcus
DegU





carnosus


2676
Two-component sensor histidine
UniRef100_Q67JE7
Symbiobacterium
DegS



kinase [Symbiobacterium

thermophilum



thermophilum]


2677
YdfQ protein [Bacillus subtilis]
UniRef100_P96695
Bacillus subtilis
YdfQ


2678
Hypothetical Membrane Spanning
UniRef100_Q813Y5
Bacillus cereus
YrkJ



Protein [Bacillus cereus]


2679
Hypothetical UPF0033 protein yrkI
UniRef100_P54436
Bacillus subtilis



[Bacillus subtilis]










2680
UPI00003CB3C6 UniRef100 entry
UniRef100_UPI00003CB3C6
YrkH











2681
Molybdopterin biosynthesis MoeB
UniRef100_Q81HL2
Bacillus cereus
YrkF



protein [Bacillus cereus]


2682
Hypothetical protein yrkE [Bacillus
UniRef100_P54432
Bacillus subtilis
YrkE



subtilis]










2683
Hypothetical conserved protein
UniRef100_Q8EN37
Oceanobacillus iheyensis



[Oceanobacillus iheyensis]











2684
S-adenosylmethionine-dependent
UniRef100_Q97FB3
Clostridium
YcgJ



methyltransferase [Clostridium

acetobutylicum



acetobutylicum]










2685
Hypothetical protein [Bacillus anthracis]
UniRef100_Q81N81
Bacillus anthracis











2686
YciB protein [Bacillus subtilis]
UniRef100_P94399
Bacillus subtilis











2687
Acetylxylan esterase related enzyme
UniRef100_Q97LM8
Clostridium acetobutylicum



[Clostridium acetobutylicum]










2688
Hypothetical UPF0161 protein
UniRef100_P61464
Bacillus cereus



BCE4947 [Bacillus cereus]










2689
Delta-aminolevulinic acid dehydratase
UniRef100_Q9K8G2
Bacillus halodurans



[Bacillus halodurans]











2690
6-phospho-3-hexuloisomerase [Bacillus
UniRef100_Q6TV53
Bacillus
HxlB



methanolicus]

methanolicus


2691
Probable hexulose-6-phosphate
UniRef100_P42405
Bacillus subtilis
HxlA



synthase [Bacillus subtilis]


2692
Transcriptional regulator [Bacillus
UniRef100_Q70KJ9
Bacillus
HxlR



amyloliquefaciens]

amyloliquefaciens


2693
Fatty acid desaturase [Bacillus subtilis]
UniRef100_O34653
Bacillus subtilis
Des


2694
Sensor kinase [Bacillus subtilis]
UniRef100_O34757
Bacillus subtilis
YocF


2695
Sensor regulator [Bacillus subtilis]
UniRef100_O34723
Bacillus subtilis
YocG










2696
UPI00003CC1E4 UniRef100 entry
UniRef100_UPI00003CC1E4
YcgT











2697
Nickel transport system [Bacillus
UniRef100_Q9KFB8
Bacillus
AppA



halodurans]

halodurans


2698
Nickel transport system [Bacillus
UniRef100_Q9KFB7
Bacillus
AppB



halodurans]

halodurans


2699
Nickel transport system [Bacillus
UniRef100_Q9KFB6
Bacillus
AppC



halodurans]

halodurans


2700
Oligopeptide ABC transporter [Bacillus
UniRef100_Q9KFB5
Bacillus
DppD



halodurans]

halodurans


2701
Oligopeptide ABC transporter [Bacillus
UniRef100_Q9KFB4
Bacillus
AppF



halodurans]

halodurans










2702
UPI00003CB880 UniRef100 entry
UniRef100_UPI00003CB880
YdfL


2703
UPI00003CA374 UniRef100 entry
UniRef100_UPI00003CA374
YoeA










2704
PROBABLE TRANSCRIPTION
UniRef100_Q8XS91
Ralstonia solanacearum



REGULATOR PROTEIN [Ralstonia



solanacearum]











2705
Short chain dehydrogenase family
UniRef100_Q834I5
Enterococcus
YvaG



protein [Enterococcus faecalis]

faecalis










2706
Uncharacterized protein, containing
UniRef100_Q97L24
Clostridium acetobutylicum



predicted phosphatase domain



[Clostridium acetobutylicum]










2707
UPI000025758C UniRef100 entry
UniRef100_UPI000025758C
PnbA











2708
Cytochrome P450 [Bacillus subtilis]
UniRef100_O08469
Bacillus subtilis
CypA


2709
YtnM [Bacillus subtilis]
UniRef100_O34430
Bacillus subtilis
YtnM


2710
Hypothetical protein yndA precursor
UniRef100_O31805
Bacillus subtilis
YndA



[Bacillus subtilis]


2711
YvaG protein [Bacillus subtilis]
UniRef100_O32229
Bacillus subtilis
YvaG


2712
Levanase precursor [Bacillus subtilis]
UniRef100_P05656
Bacillus subtilis
SacC


2713
PTS system, fructose-specific IID
UniRef100_P26382
Bacillus subtilis
LevG



component [Bacillus subtilis]


2714
PTS system, fructose-specific IIC
UniRef100_P26381
Bacillus subtilis
LevF



component [Bacillus subtilis]


2715
PTS system, fructose-specific IIB
UniRef100_P26380
Bacillus subtilis
LevE



component [Bacillus subtilis]


2716
PTS system, fructose-specific IIA
UniRef100_P26379
Bacillus subtilis
LevD



component [Bacillus subtilis]


2717
Transcriptional regulatory protein levR
UniRef100_P23914
Bacillus subtilis
LevR



[Bacillus subtilis]


2718
Hypothetical protein [Bacillus cereus]
UniRef100_Q734Q5
Bacillus cereus


2719
Hypothetical protein yrhK [Bacillus
UniRef100_O05401
Bacillus subtilis



subtilis]










2720
UPI00003CB785 UniRef100 entry
UniRef100_UPI00003CB785












2721



AdaA


2722
Methylated-DNA-protein-cysteine S-
UniRef100_Q732Y7
Bacillus cereus
AdaB



methyltransferase [Bacillus cereus]


2723
Oxidoreductase, aldo/keto reductase
UniRef100_Q6HBJ5
Bacillus
YtbE



family [Bacillus thuringiensis]

thuringiensis


2724



YtbD


2725
Hypothetical UPF0087 protein ytcD
UniRef100_O34533
Bacillus subtilis
YtcD



[Bacillus subtilis]


2726


2727
Hypothetical protein yjjA [Bacillus
UniRef100_O34394
Bacillus subtilis
YjjA



subtilis]










2728
UPI000028298B UniRef100 entry
UniRef100_UPI000028298B












2729



YxeB


2730
Putative HTH-type transcriptional
UniRef100_P37499
Bacillus subtilis
YybE



regulator yybE [Bacillus subtilis]


2731
Hypothetical transport protein yybF
UniRef100_P37498
Bacillus subtilis
YybF



[Bacillus subtilis]


2732
Hypothetical protein [Bacillus cereus]
UniRef100_Q732J0
Bacillus cereus


2733
Probable bifunctional P-450: NADPH-
UniRef100_O08336
Includes:
YrhJ



P450 reductase 2 [Includes:

Cytochrome



Cytochrome P450 102 (EC 1.14.14.1);

P450 102 (EC



NADPH--cytochrome P450 reductase

1.14.14.1);



(EC 1.6.2.4)] [Bacillus subtilis]

NADPH--





cytochrome





P450 reductase





(EC 1.6.2.4)


2734
Regulatory protein [Bacillus subtilis]
UniRef100_O08335
Bacillus subtilis
YrhI


2735



WprA


2736
YrhH [Bacillus subtilis]
UniRef100_O05400
Bacillus subtilis
YrhH


2737


2738


2739


2740
Cystathionine gamma-lyase [Bacillus
UniRef100_O05394
Bacillus subtilis
YrhB



subtilis]


2741
Cysteine synthase [Bacillus subtilis]
UniRef100_O05393
Bacillus subtilis
YrhA


2742
MTA/SAH nucleosidase [Bacillus
UniRef100_O32028
Bacillus subtilis
Mtn



subtilis]


2743
YrrT protein [Bacillus subtilis]
UniRef100_O32029
Bacillus subtilis
YrrT


2744
Hypothetical protein yrzA [Bacillus
UniRef100_O32030
Bacillus subtilis



subtilis]


2745



YrrS


2746
YrrR protein [Bacillus subtilis]
UniRef100_O32032
Bacillus subtilis
YrrR


2747
Transcription elongation factor greA
UniRef100_P80240
Bacillus subtilis
GreA



[Bacillus subtilis]


2748
Uridine kinase [Bacillus subtilis]
UniRef100_O32033
Bacillus subtilis
Udk


2749
YrrO protein [Bacillus subtilis]
UniRef100_O32034
Bacillus subtilis
YrrO


2750
YrrN protein [Bacillus subtilis]
UniRef100_O32035
Bacillus subtilis
YrrN


2751
YrrM protein [Bacillus subtilis]
UniRef100_O32036
Bacillus subtilis
YrrM


2752
YrrL protein [Bacillus subtilis]
UniRef100_O34758
Bacillus subtilis
YrrL


2753
YrzB protein [Bacillus subtilis]
UniRef100_O34828
Bacillus subtilis


2754
Putative Holliday junction resolvase
UniRef100_O34634
Bacillus subtilis
YrrK



[Bacillus subtilis]


2755
Hypothetical UPF0297 protein yrzL
UniRef100_Q7WY61
Bacillus subtilis



[Bacillus subtilis]


2756
Alanyl-tRNA synthetase [Bacillus
UniRef100_O34526
Bacillus subtilis
AlaS



subtilis]


2757
Hypothetical UPF0118 protein yrrI
UniRef100_O34472
Bacillus subtilis
YrrI



[Bacillus subtilis]


2758










2759
Hypothetical protein [Bacillus cereus
UniRef100_Q634F2
Bacillus cereus ZK



ZK]











2760
YrrD protein [Bacillus subtilis]
UniRef100_O34402
Bacillus subtilis
YrrD


2761
YrrC protein [Bacillus subtilis]
UniRef100_O34481
Bacillus subtilis
YrrC


2762
YrrB protein [Bacillus subtilis]
UniRef100_O34452
Bacillus subtilis
YrrB


2763
Probable tRNA (5-methylaminomethyl-
UniRef100_O35020
Bacillus subtilis
TrmU



2-thiouridylate)-methyltransferase



[Bacillus subtilis]


2764
YrvO protein [Bacillus subtilis]
UniRef100_O34599
Bacillus subtilis
YrvO










2765
BH1259 protein [Bacillus halodurans]
UniRef100_Q9KDF4
Bacillus halodurans











2766
YrvN protein [Bacillus subtilis]
UniRef100_O34528
Bacillus subtilis
YrvN


2767
YrvM protein [Bacillus subtilis]
UniRef100_O32037
Bacillus subtilis
YrvM


2768
Aspartyl-tRNA synthetase [Bacillus
UniRef100_O32038
Bacillus subtilis
AspS



subtilis]


2769
Histidyl-tRNA synthetase [Bacillus
UniRef100_O32039
Bacillus subtilis
HisS



subtilis]


2770


2771



YrvJ


2772
Putative D-tyrosyl-tRNA(Tyr)
UniRef100_O32042
Bacillus subtilis
YrvI



deacylase-like protein [Bacillus subtilis]


2773
GTP pyrophosphokinase (EC 2.7.6.5)
UniRef100_O54408
Bacillus subtilis
RelA



(ATP:GTP 3′-pyrophosphotransferase)



(ppGpp synthetase I) ((P)ppGpp



synthetase) [Bacillus subtilis]


2774
Adenine phosphoribosyltransferase
UniRef100_O34443
Bacillus subtilis
Apt



[Bacillus subtilis]


2775
YrvE protein [Bacillus subtilis]
UniRef100_O32044
Bacillus subtilis
YrvE


2776
YrvD protein [Bacillus subtilis]
UniRef100_O32045
Bacillus subtilis


2777
SecDF protein [Bacillus subtilis]
UniRef100_O32047
Bacillus subtilis
SecDF


2778
YrzD protein [Bacillus subtilis]
UniRef100_O32049
Bacillus subtilis


2779



SpoVB


2780
Hypothetical protein yrbG [Bacillus
UniRef100_O32050
Bacillus subtilis
YrbG



subtilis]


2781
YrzE protein [Bacillus subtilis]
UniRef100_O32051
Bacillus subtilis
YrzE


2782
Hypothetical UPF0092 protein yrbF
UniRef100_O32052
Bacillus subtilis



[Bacillus subtilis]


2783
Queuine tRNA-ribosyltransferase
UniRef100_O32053
Bacillus subtilis
Tgt



[Bacillus subtilis]


2784
S-adenosylmethionine:tRNA
UniRef100_O32054
Bacillus subtilis
QueA



ribosyltransferase-isomerase [Bacillus



subtilis]


2785


2786
Holliday junction DNA helicase ruvB
UniRef100_O32055
Bacillus subtilis
RuvB



[Bacillus subtilis]


2787
Holliday junction DNA helicase ruvA
UniRef100_O05392
Bacillus subtilis
RuvA



[Bacillus subtilis]


2788
BofC protein precursor [Bacillus
UniRef100_O05391
Bacillus subtilis
BofC



subtilis]


2789
Hypothetical conserved protein
UniRef100_Q8ERL7
Oceanobacillus
YrzF



[Oceanobacillus iheyensis]

iheyensis


2790










2791
Small, acid-soluble spore protein H
UniRef100_Q9KB75
Bacillus halodurans



[Bacillus halodurans]











2792
Hypothetical protein yjoA [Bacillus
UniRef100_O34334
Bacillus subtilis
YjoA



subtilis]


2793
YmaC protein [Bacillus subtilis]
UniRef100_O31789
Bacillus subtilis
YmaC


2794


2795


2796
Hypothetical UPF0082 protein yrbC
UniRef100_P94447
Bacillus subtilis
YrbC



[Bacillus subtilis]


2797
Sporulation cortex protein coxA
UniRef100_P94446
Bacillus subtilis
CoxA



[Bacillus subtilis]


2798
Morphogenetic protein associated with
UniRef100_O32062
Bacillus subtilis
SafA



SpoVID [Bacillus subtilis]


2799
Quinolinate synthetase A [Bacillus
UniRef100_Q9KWZ1
Bacillus subtilis
NadA



subtilis]


2800
Probable nicotinate-nucleotide
UniRef100_P39666
carboxylating
NadC



pyrophosphorylase [carboxylating]



[Bacillus subtilis]


2801
L-aspartate oxidase [Bacillus subtilis]
UniRef100_P38032
Bacillus subtilis
NadB


2802
Probable cysteine desulfurase [Bacillus
UniRef100_P38033
Bacillus subtilis
NifS



subtilis]


2803



YrxA


2804
Prephenate dehydratase [Bacillus
UniRef100_P21203
Bacillus subtilis
PheA



subtilis]


2805
ACT domain protein pheB [Bacillus
UniRef100_P21204
Bacillus subtilis
PheB



subtilis]


2806
Spo0B-associated GTP-binding protein
UniRef100_Q659J4
Bacillus
Obg



[Bacillus amyloliquefaciens]

amyloliquefaciens


2807
Sporulation initiation
UniRef100_P06535
Bacillus subtilis
Spo0B



phosphotransferase B [Bacillus subtilis]


2808
50S ribosomal protein L27 [Bacillus
UniRef100_P05657
Bacillus subtilis



subtilis]


2809


2810
50S ribosomal protein L21 [Bacillus
UniRef100_P26908
Bacillus subtilis
RplU



subtilis]


2811
Stage IV sporulation protein FB
UniRef100_P26937
Bacillus subtilis
SpoIVFB



[Bacillus subtilis]


2812
Stage IV sporulation protein FA
UniRef100_P26936
Bacillus subtilis
SpoIVFA



[Bacillus subtilis]


2813
Hypothetical protein [Bacillus cereus]
UniRef100_Q816V6
Bacillus cereus
YndB










2814
Transcriptional regulator, ArsR family
UniRef100_Q632Y0
Bacillus cereus ZK



[Bacillus cereus ZK]











2815
Septum site-determining protein minD
UniRef100_Q01464
Bacillus subtilis
MinD



[Bacillus subtilis]


2816



MinC


2817
Rod shape-determining protein mreD
UniRef100_Q01467
Bacillus subtilis
MreD



[Bacillus subtilis]


2818
Rod shape-determining protein mreC
UniRef100_Q01466
Bacillus subtilis
MreC



[Bacillus subtilis]


2819
Rod shape-determining protein mreB
UniRef100_Q01465
Bacillus subtilis
MreB



[Bacillus subtilis]


2820
DNA repair protein radC homolog
UniRef100_Q02170
Bacillus subtilis
RadC



[Bacillus subtilis]


2821
Septum formation protein Maf [Bacillus
UniRef100_Q02169
Bacillus subtilis
Maf



subtilis]


2822
Stage II sporulation protein B [Bacillus
UniRef100_P37575
Bacillus subtilis
SpoIIB



subtilis]


2823
Type 4 prepilin-like proteins leader
UniRef100_P15378
Includes: Leader
ComC



peptide processing enzyme (Late

peptidase (EC



competence protein comC) [Includes:

3.4.23.43)



Leader peptidase (EC 3.4.23.43)

(Prepilin



(Prepilin peptidase); N-

peptidase); N-



methyltransferase (EC 2.1.1.—)]

methyltransferase



[Bacillus subtilis]

(EC 2.1.1.—)


2824



FolC


2825
Valyl-tRNA synthetase [Bacillus
UniRef100_Q05873
Bacillus subtilis
ValS



subtilis]










2826
Hypothetical protein OB2062
UniRef100_Q8EPN1
Oceanobacillus iheyensis



[Oceanobacillus iheyensis]











2827
Hypothetical protein ysxE [Bacillus
UniRef100_P37964
Bacillus subtilis
YsxE



subtilis]


2828
Stage VI sporulation protein D [Bacillus
UniRef100_P37963
Bacillus subtilis
SpoVID



subtilis]


2829
Glutamate-1-semialdehyde 2,1-
UniRef100_P30949
Bacillus subtilis
HemL



aminomutase [Bacillus subtilis]


2830
Delta-aminolevulinic acid dehydratase
UniRef100_P30950
Bacillus subtilis
HemB



[Bacillus subtilis]


2831
Uroporphyrinogen-III synthase [Bacillus
UniRef100_P21248
Bacillus subtilis
HemD



subtilis]


2832
Porphobilinogen deaminase [Bacillus
UniRef100_P16616
Bacillus subtilis
HemC



subtilis]


2833
Protein hemX [Bacillus subtilis]
UniRef100_P16645
Bacillus subtilis
HemX


2834
Glutamyl-tRNA reductase [Bacillus
UniRef100_P16618
Bacillus subtilis
HemA



subtilis]


2835
Hypothetical protein ysxD [Bacillus
UniRef100_P40736
Bacillus subtilis
YsxD



subtilis]


2836
Probable GTP-binding protein engB
UniRef100_P38424
Bacillus subtilis
YsxC



[Bacillus subtilis]


2837
ATP-dependent protease La 1 [Bacillus
UniRef100_P37945
Bacillus subtilis
LonA



subtilis]


2838
ATP-dependent protease La homolog
UniRef100_P42425
Bacillus subtilis
LonB



[Bacillus subtilis]


2839
ATP-dependent Clp protease ATP-
UniRef100_P50866
Bacillus subtilis
ClpX



binding subunit clpX [Bacillus subtilis]


2840
Trigger factor [Bacillus subtilis]
UniRef100_P80698
Bacillus subtilis
Tig


2841
Hypothetical protein ysoA [Bacillus
UniRef100_P94569
Bacillus subtilis
YsoA



subtilis]


2842
3-isopropylmalate dehydratase small
UniRef100_P94568
Bacillus subtilis
LeuD



subunit [Bacillus subtilis]


2843
3-isopropylmalate dehydratase large
UniRef100_P80858
Bacillus subtilis
LeuC



subunit [Bacillus subtilis]


2844



LeuB


2845
2-isopropylmalate synthase [Bacillus
UniRef100_P94565
Bacillus subtilis
LeuA



subtilis]


2846
Ketol-acid reductoisomerase [Bacillus
UniRef100_P37253
Bacillus subtilis
IlvC



subtilis]


2847
Acetolactate synthase small subunit
UniRef100_P37252
Bacillus subtilis
IlvH



[Bacillus subtilis]


2848
Acetolactate synthase large subunit
UniRef100_P37251
Bacillus subtilis
IlvB



[Bacillus subtilis]


2849
Branched-chain amino acid
UniRef100_Q6HLF7
Bacillus
Dat



aminotransferase [Bacillus

thuringiensis



thuringiensis]


2850


2851



RocG










2852
BH3337 protein [Bacillus halodurans]
UniRef100_Q9K7M4
Bacillus halodurans











2853



YxeD


2854
Hypothetical protein yqbA [Bacillus
UniRef100_P45917
Bacillus subtilis



subtilis]


2855
Hypothetical protein yqaT [Bacillus
UniRef100_P45916
Bacillus subtilis
YqaT



subtilis]


2856
Lin1266 protein [Listeria innocua]
UniRef100_Q92CC3
Listeria innocua


2857
Lin1733 protein [Listeria innocua]
UniRef100_Q92B18
Listeria innocua


2858


2859


2860


2861



MtbP










2862
BH3535 protein [Bacillus halodurans]
UniRef100_Q9K738
Bacillus halodurans











2863



YeeF


2864



YxiD


2865



RapI


2866


2867


2868


2869
Hypothetical UPF0025 protein ysnB
UniRef100_P94559
Bacillus subtilis
YsnB



[Bacillus subtilis]


2870
HAM1 protein homolog [Bacillus
UniRef100_P94558
Bacillus subtilis
YsnA



subtilis]


2871



Rph


2872
Germination protein gerM [Bacillus
UniRef100_P39072
Bacillus subtilis
GerM



subtilis]


2873



RacE


2874
Hypothetical protein ysmB [Bacillus
UniRef100_P97247
Bacillus subtilis
YsmB



subtilis]


2875
Germination protein gerE [Bacillus
UniRef100_P11470
Bacillus subtilis



subtilis]


2876
Oxidoreductase [Clostridium
UniRef100_Q97TP7
Clostridium
YqjQ



acetobutylicum]

acetobutylicum


2877
Hypothetical protein ysmA [Bacillus
UniRef100_Q6L874
Bacillus subtilis
YsmA



subtilis]


2878
Succinate dehydrogenase iron-sulfur
UniRef100_P08066
Bacillus subtilis
SdhB



protein [Bacillus subtilis]


2879



SdhA


2880


2881
Succinate dehydrogenase cytochrome
UniRef100_P08064
Bacillus subtilis
SdhC



B-558 subunit [Bacillus subtilis]


2882
Hypothetical protein yslB [Bacillus
UniRef100_P42955
Bacillus subtilis
YslB



subtilis]


2883
Aspartokinase 2 (EC 2.7.2.4)
UniRef100_P08495
Contains:
LysC



(Aspartokinase II) (Aspartate kinase 2)

Aspartokinase II



[Contains: Aspartokinase II alpha

alpha subunit;



subunit; Aspartokinase II beta subunit]

Aspartokinase II



[Bacillus subtilis]

beta subunit


2884
UvrABC system protein C [Bacillus
UniRef100_P14951
Bacillus subtilis
UvrC



subtilis]


2885
Thioredoxin [Bacillus subtilis]
UniRef100_P14949
Bacillus subtilis
TrxA


2886
Electron transfer flavoprotein alpha-
UniRef100_P94551
Bacillus subtilis
EtfA



subunit [Bacillus subtilis]


2887
Electron transfer flavoprotein beta-
UniRef100_P94550
Bacillus subtilis
EtfB



subunit [Bacillus subtilis]


2888
Hypothetical protein ysiB [Bacillus
UniRef100_P94549
Bacillus subtilis
YsiB



subtilis]


2889
Hypothetical protein ysiA [Bacillus
UniRef100_P94548
Bacillus subtilis
YsiA



subtilis]


2890
Long-chain-fatty-acid-CoA ligase
UniRef100_P94547
Bacillus subtilis
LcfA



[Bacillus subtilis]


2891
Hypothetical protein yshE [Bacillus
UniRef100_P94546
Bacillus subtilis
YshE



subtilis]


2892
MutS2 protein [Bacillus subtilis]
UniRef100_P94545
Bacillus subtilis
MutSB


2893
Hypothetical protein yshC [Bacillus
UniRef100_P94544
Bacillus subtilis
YshC



subtilis]


2894



YshB


2895


2896
Ribonuclease HIII [Bacillus subtilis]
UniRef100_P94541
Bacillus subtilis
RnhC


2897


2898


2899


2900


2901



YxlF


2902


2903
Phenylalanyl-tRNA synthetase beta
UniRef100_P17922
Bacillus subtilis
PheT



chain [Bacillus subtilis]


2904
Phenylalanyl-tRNA synthetase alpha
UniRef100_Q659J3
Bacillus
PheS



chain [Bacillus amyloliquefaciens]

amyloliquefaciens


2905
Hypothetical protein ysgA [Bacillus
UniRef100_P94538
Bacillus subtilis
YsgA



subtilis]


2906
Small, acid-soluble spore protein I
UniRef100_P94537
Bacillus subtilis



[Bacillus subtilis]


2907
Carbon starvation protein A homolog
UniRef100_P94532
Bacillus subtilis
CstA



[Bacillus subtilis]


2908
Alpha-N-arabinofuranosidase [Bacillus
UniRef100_Q9XBQ3
Bacillus
AbfA



stearothermophilus]

stearothermophilus


2909
L-arabinose transport system
UniRef100_P94530
Bacillus subtilis
AraQ



permease protein araQ [Bacillus



subtilis]


2910
L-arabinose transport system
UniRef100_P94529
Bacillus subtilis
AraP



permease protein araP [Bacillus



subtilis]


2911
Probable arabinose-binding protein
UniRef100_P94528
Bacillus subtilis
AraN



precursor [Bacillus subtilis]


2912
Arabinose operon protein araM
UniRef100_P94527
Bacillus subtilis
AraM



[Bacillus subtilis]


2913
L-ribulose-5-phosphate 4-epimerase
UniRef100_P94525
Bacillus subtilis
AraD



[Bacillus subtilis]


2914
Ribulokinase [Bacillus subtilis]
UniRef100_P94524
Bacillus subtilis
AraB


2915
L-arabinose isomerase [Bacillus
UniRef100_P94523
Bacillus subtilis
AraA



subtilis]


2916



AbnA


2917
Hypothetical protein ysdC [Bacillus
UniRef100_P94521
Bacillus subtilis
YsdC



subtilis]


2918
Hypothetical protein ysdB [Bacillus
UniRef100_P94520
Bacillus subtilis
YsdB



subtilis]


2919
Hypothetical protein ysdA [Bacillus
UniRef100_P94519
Bacillus subtilis



subtilis]


2920
50S ribosomal protein L20 [Bacillus
UniRef100_P55873
Bacillus subtilis
RplT



subtilis]


2921
50S ribosomal protein L35 [Bacillus
UniRef100_P55874
Bacillus subtilis



subtilis]


2922



InfC


2923
Antiholin-like protein IrgB [Bacillus
UniRef100_P94516
Bacillus subtilis
YsbB



subtilis]


2924
Antiholin-like protein IrgA [Bacillus
UniRef100_P94515
Bacillus subtilis
YsbA



subtilis]


2925



PhoD


2926
Sensory transduction protein lytT
UniRef100_P94514
Bacillus subtilis
LytT



[Bacillus subtilis]


2927
Sensor protein lytS [Bacillus subtilis]
UniRef100_P94513
Bacillus subtilis
LytS










2928
Hypothetical protein [Bacillus anthracis]
UniRef100_Q81N00
Bacillus anthracis











2929
Hypothetical protein ysaA [Bacillus
UniRef100_P94512
Bacillus subtilis
YsaA



subtilis]


2930
Threonyl-tRNA synthetase 1 [Bacillus
UniRef100_P18255
Bacillus subtilis
ThrS



subtilis]


2931
Hypothetical protein ytxC [Bacillus
UniRef100_P06569
Bacillus subtilis
YtxC



subtilis]


2932
Hypothetical UPF0043 protein ytxB
UniRef100_P06568
Bacillus subtilis
YtxB



[Bacillus subtilis]


2933
Primosomal protein dnal [Bacillus
UniRef100_P06567
Bacillus subtilis
DnaI



subtilis]


2934
Replication initiation and membrane
UniRef100_P07908
Bacillus subtilis
DnaB



attachment protein [Bacillus subtilis]


2935
Hypothetical UPF0168 protein ytcG
UniRef100_Q45549
Bacillus subtilis
YtcG



[Bacillus subtilis]


2936


2937



SpeD


2938



GapB


2939
Pectin lyase [Bacillus subtilis]
UniRef100_P94449
Bacillus subtilis
PelB


2940
Dephospho-CoA kinase [Bacillus
UniRef100_O34932
Bacillus subtilis
YtaG



subtilis]


2941



YtaF


2942
Formamidopyrimidine-DNA glycosylase
UniRef100_O34403
Bacillus subtilis
MutM



[Bacillus subtilis]


2943
DNA polymerase I [Bacillus subtilis]
UniRef100_O34996
Bacillus subtilis
PolA


2944
Alkaline phosphatase synthesis sensor
UniRef100_P23545
Bacillus subtilis
PhoR



protein phoR [Bacillus subtilis]


2945
Alkaline phosphatase synthesis
UniRef100_P13792
Bacillus subtilis
PhoP



transcriptional regulatory protein phoP



[Bacillus subtilis]


2946
Malate dehydrogenase [Bacillus
UniRef100_P49814
Bacillus subtilis
Mdh



subtilis]


2947
Isocitrate dehydrogenase [NADP]
UniRef100_P39126
NADP
Icd



[Bacillus subtilis]


2948
Citrate synthase II [Bacillus subtilis]
UniRef100_P39120
Bacillus subtilis
CitZ


2949
YtwI [Bacillus subtilis]
UniRef100_O34811
Bacillus subtilis
YtwI


2950
Hypothetical UPF0118 protein ytvI
UniRef100_O34991
Bacillus subtilis
YtvI



[Bacillus subtilis]


2951
YtzA protein [Bacillus subtilis]
UniRef100_O32064
Bacillus subtilis
YtzA


2952



Pyk


2953
6-phosphofructokinase [Bacillus
UniRef100_O34529
Bacillus subtilis
PfkA



subtilis]


2954
Acetyl-coenzyme A carboxylase
UniRef100_O34847
Bacillus subtilis
AccA



carboxyl transferase subunit alpha



[Bacillus subtilis]


2955
Acetyl-CoA carboxylase subunit
UniRef100_O34571
Bacillus subtilis
AccD



[Bacillus subtilis]


2956



YtsJ


2957
DNA polymerase III alpha subunit
UniRef100_O34623
Bacillus subtilis
DnaE



[Bacillus subtilis]


2958
Hypothetical Membrane Spanning
UniRef100_Q812P3
Bacillus cereus



Protein [Bacillus cereus]


2959
YtrI [Bacillus subtilis]
UniRef100_O34460
Bacillus subtilis
YtrI










2960
BH3172 protein [Bacillus halodurans]
UniRef100_Q9K835
Bacillus halodurans











2961
YtqI [Bacillus subtilis]
UniRef100_O34600
Bacillus subtilis
YtqI


2962
YtpI [Bacillus subtilis]
UniRef100_O34922
Bacillus subtilis


2963



YtoI


2964



PadR


2965
YtkL protein [Bacillus subtilis]
UniRef100_Q795U4
Bacillus subtilis
YtkL


2966



YtkK


2967


2968
Argininosuccinate lyase [Bacillus
UniRef100_O34858
Bacillus subtilis
ArgH



subtilis]


2969
Argininosuccinate synthase [Bacillus
UniRef100_O34347
Bacillus subtilis
ArgG



subtilis]


2970
Molybdenum cofactor biosynthesis
UniRef100_O34457
Bacillus subtilis
MoaB



protein B [Bacillus subtilis]


2971



AckA


2972
Hypothetical protein ytxK [Bacillus
UniRef100_P37876
Bacillus subtilis
YtxK



subtilis]


2973
Probable thiol peroxidase [Bacillus
UniRef100_P80864
Bacillus subtilis
Tpx



subtilis]


2974
YtfJ [Bacillus subtilis]
UniRef100_O34806
Bacillus subtilis
YtfJ


2975



YtfI


2976
YteJ [Bacillus subtilis]
UniRef100_O34424
Bacillus subtilis
YteJ


2977
Putative signal peptide peptidase sppA
UniRef100_O34525
Bacillus subtilis
SppA



[Bacillus subtilis]


2978
Probable inorganic
UniRef100_O34934
Bacillus subtilis
YtdI



polyphosphate/ATP-NAD kinase 2 (EC



2.7.1.23) (Poly(P)/ATP NAD kinase 2)



[Bacillus subtilis]


2979
YhbJ protein [Bacillus subtilis]
UniRef100_O31593
Bacillus subtilis
YhbJ


2980
Multidrug resistance protein
UniRef100_Q8CQB1
Staphylococcus
YubD



[Staphylococcus epidermidis]

epidermidis


2981
Putative HTH-type transcriptional
UniRef100_P42103
Bacillus subtilis
YxaD



regulator yxaD [Bacillus subtilis]


2982
YtcI [Bacillus subtilis]
UniRef100_O34613
Bacillus subtilis
YtcI










2983
Small, acid-soluble spore protein 1
UniRef100_P06552
Bacillus stearothermophilus



[Bacillus stearothermophilus]











2984
Probable thiamine biosynthesis protein
UniRef100_O34595
Bacillus subtilis
YtbJ



thil [Bacillus subtilis]


2985
NifS2 [Bacillus subtilis]
UniRef100_O34874
Bacillus subtilis
NifZ


2986
Branched-chain amino acid transport
UniRef100_O34545
Bacillus subtilis
BraB



system carrier protein braB [Bacillus



subtilis]










2987
IS1627s1-related, transposase
UniRef100_Q7CMD0
Bacillus anthracis str. A2012



[Bacillus anthracis str. A2012]










2988
UPI00003CC069 UniRef100 entry
UniRef100_UPI00003CC069












2989
Septation ring formation regulator ezrA
UniRef100_O34894
Bacillus subtilis
EzrA



[Bacillus subtilis]


2990
Histidinol-phosphatase [Bacillus
UniRef100_O34411
Bacillus subtilis
HisJ



subtilis]


2991
Probable HTH-type transcriptional
UniRef100_O34970
Bacillus subtilis
YttP



regulator yttP [Bacillus subtilis]










2992
Hypothetical conserved protein
UniRef100_Q8EPB0
Oceanobacillus iheyensis



[Oceanobacillus iheyensis]











2993
YtrP [Bacillus subtilis]
UniRef100_O34325
Bacillus subtilis
YtrP


2994
30S ribosomal protein S4 [Bacillus
UniRef100_P21466
Bacillus subtilis
RpsD



subtilis]


2995


2996


2997



YddR


2998
HTH-type transcriptional regulator lrpA
UniRef100_P96652
Bacillus subtilis
LrpA



[Bacillus subtilis]


2999


3000
Tyrosyl-tRNA synthetase 1 [Bacillus
UniRef100_P22326
Bacillus subtilis
TyrS



subtilis]


3001
Acetyl-coenzyme A synthetase
UniRef100_P39062
Bacillus subtilis
AcsA



[Bacillus subtilis]


3002
Acetoin utilization protein acuA
UniRef100_P39065
Bacillus subtilis
AcuA



[Bacillus subtilis]


3003
Acetoin utilization acuB protein
UniRef100_P39066
Bacillus subtilis
AcuB



[Bacillus subtilis]


3004
Acetoin utilization protein acuC
UniRef100_P39067
Bacillus subtilis
AcuC



[Bacillus subtilis]


3005
Hypothetical protein ytxE [Bacillus
UniRef100_P39064
Bacillus subtilis
YtxE



subtilis]


3006
Hypothetical protein ytxD [Bacillus
UniRef100_P39063
Bacillus subtilis
YtxD



subtilis]


3007
Catabolite control protein A [Bacillus
UniRef100_P25144
Bacillus subtilis
CcpA



subtilis]


3008
AroA(G) protein [Includes: Phospho-2-
UniRef100_P39912
Includes:
AroA



dehydro-3-deoxyheptonate aldolase

Phospho-2-



(EC 2.5.1.54) (Phospho-2-keto-3-

dehydro-3-



deoxyheptonate aldolase) (DAHP

deoxyheptonate



synthetase) (3-deoxy-D-arabino-

aldolase (EC



heptulosonate 7-phosphate synthase);

2.5.1.54)



Chorismate mutase (EC 5.4.99.5)]

(Phospho-2-



[Bacillus subtilis]

keto-3-





deoxyheptonate





aldolase) (DAHP





synthetase) (3-





deoxy-D-arabino-





heptulosonate 7-





phosphate





synthase);





Chorismate





mutase (EC





5.4.99.5)










3009
Similar to hypothetical repeat
UniRef100_Q7N3B8
Photorhabdus luminescens



containing protein [Photorhabdus



luminescens]











3010
Hypothetical protein ytxJ [Bacillus
UniRef100_P39914
Bacillus subtilis
YtxJ



subtilis]


3011
Hypothetical protein ytxH [Bacillus
UniRef100_P40780
Bacillus subtilis
YtxH



subtilis]


3012
Hypothetical protein ytxG [Bacillus
UniRef100_P40779
Bacillus subtilis
YtxG



subtilis]


3013



MurC


3014
YtpT [Bacillus subtilis]
UniRef100_O34749
Bacillus subtilis
YtpT


3015
YtpR [Bacillus subtilis]
UniRef100_O34943
Bacillus subtilis
YtpR


3016
YtpQ [Bacillus subtilis]
UniRef100_O34496
Bacillus subtilis
YtpQ


3017
Putative thioredoxin [Bacillus subtilis]
UniRef100_O34357
Bacillus subtilis
YtpP


3018
YtoQ [Bacillus subtilis]
UniRef100_O34305
Bacillus subtilis
YtoQ


3019
YtoP [Bacillus subtilis]
UniRef100_O34924
Bacillus subtilis
YtoP


3020
YtzB protein [Bacillus subtilis]
UniRef100_O32065
Bacillus subtilis
YtzB


3021
Probable NAD-dependent malic
UniRef100_O34389
Bacillus subtilis
MalS



enzyme 3 [Bacillus subtilis]


3022
YtnP [Bacillus subtilis]
UniRef100_O34760
Bacillus subtilis
YtnP


3023
tRNA (guanine-N(7)-)-
UniRef100_O34522
Bacillus subtilis
YtmQ



methyltransferase (EC 2.1.1.33)



(tRNA(m7G46)-methyltransferase)



[Bacillus subtilis]


3024
YtzH protein [Bacillus subtilis]
UniRef100_O32066
Bacillus subtilis


3025
YtmP [Bacillus subtilis]
UniRef100_O34935
Bacillus subtilis
YtmP


3026
AmyX protein [Bacillus subtilis]
UniRef100_O34587
Bacillus subtilis
AmyX


3027
YtlR [Bacillus subtilis]
UniRef100_O34799
Bacillus subtilis
YtlR


3028
YtlQ [Bacillus subtilis]
UniRef100_O34471
Bacillus subtilis
YtlQ


3029
Hypothetical UPF0097 protein ytlP
UniRef100_O34570
Bacillus subtilis
YtlP



[Bacillus subtilis]


3030
Probable cysteine synthase (EC
UniRef100_O34476
Bacillus subtilis
YtkP



2.5.1.47) (O-acetylserine sulfhydrylase)



(O-acetylserine (Thiol)-lyase) [Bacillus



subtilis]


3031
Hypothetical protein [Bacillus cereus]
UniRef100_Q81BR8
Bacillus cereus
YncE


3032
Putative peptidase [Bacillus subtilis]
UniRef100_O34944
Bacillus subtilis
YtjP


3033
YtiP [Bacillus subtilis]
UniRef100_O34978
Bacillus subtilis
YtiP


3034
YtzE protein [Bacillus subtilis]
UniRef100_O32067
Bacillus subtilis


3035
Ribosomal small subunit pseudouridine
UniRef100_Q816W1
Bacillus cereus
YtzF



synthase A [Bacillus cereus]


3036
YtgP [Bacillus subtilis]
UniRef100_O34674
Bacillus subtilis
YtgP


3037
YtfP [Bacillus subtilis]
UniRef100_O30505
Bacillus subtilis
YtfP


3038



OpuD


3039
Protein cse60 [Bacillus subtilis]
UniRef100_P94496
Bacillus subtilis


3040
Rhodanese-like domain protein
UniRef100_Q72YZ9
Bacillus cereus



[Bacillus cereus]


3041



RapA










3042
Hypothetical protein [Bacillus
UniRef100_Q6HI31
Bacillus thuringiensis



thuringiensis]











3043
YteU [Bacillus subtilis]
UniRef100_O34378
Bacillus subtilis
YteU


3044



YteT


3045



YteS


3046
YteR [Bacillus subtilis]
UniRef100_O34559
Bacillus subtilis
YteR


3047
Transmembrane lipoprotein [Bacillus
UniRef100_Q9KFJ5
Bacillus
LplB



halodurans]

halodurans


3048
YtdP protein [Bacillus subtilis]
UniRef100_O32071
Bacillus subtilis
YtdP


3049
YtcQ protein [Bacillus subtilis]
UniRef100_Q795R2
Bacillus subtilis
YtcQ


3050



YtcP


3051
Hypothetical protein ytbQ [Bacillus
UniRef100_P53560
Bacillus subtilis
YtbQ



subtilis]


3052
YtaP [Bacillus subtilis]
UniRef100_O34973
Bacillus subtilis
YtaP


3053
Amino acid/polyamine transporter;
UniRef100_Q6LYX9
Methanococcus
YecA



family I [Methanococcus maripaludis]

maripaludis


3054
Transcriptional regulator, LysR family
UniRef100_Q97DX1
Clostridium
YwqM



[Clostridium acetobutylicum]

acetobutylicum


3055
Prolyl endopeptidase [Bacillus cereus]
UniRef100_Q81C54
Bacillus cereus
YycE


3056
Leucyl-tRNA synthetase [Bacillus
UniRef100_P36430
Bacillus subtilis
LeuS



subtilis]


3057



YtvB


3058
YttB [Bacillus subtilis]
UniRef100_O34546
Bacillus subtilis
YttB


3059
Lipoprotein [Oceanobacillus iheyensis]
UniRef100_Q8EPK3
Oceanobacillus
YusA





iheyensis


3060
YttA [Bacillus subtilis]
UniRef100_O30500
Bacillus subtilis
YttA


3061
YtrF [Bacillus subtilis]
UniRef100_O35005
Bacillus subtilis
YtrF


3062
Hypothetical ABC transporter ATP-
UniRef100_O34392
Bacillus subtilis
YtrE



binding protein ytrE [Bacillus subtilis]


3063
YtrC [Bacillus subtilis]
UniRef100_O34898
Bacillus subtilis
YtrC


3064
Transporter [Bacillus subtilis]
UniRef100_O34641
Bacillus subtilis
YtrB


3065
Transcription regulator [Bacillus
UniRef100_O34712
Bacillus subtilis
YtrA



subtilis]


3066
Hypothetical protein ytzC [Bacillus
UniRef100_O32073
Bacillus subtilis



subtilis]


3067
YtqA [Bacillus subtilis]
UniRef100_O35008
Bacillus subtilis
YtqA


3068



YtqB


3069
Proton glutamate symport protein
UniRef100_P39817
Bacillus subtilis
GltP



[Bacillus subtilis]


3070
Hypothetical protein ytpB [Bacillus
UniRef100_O34707
Bacillus subtilis
YtpB



subtilis]


3071
Probable lysophospholipase [Bacillus
UniRef100_O34705
Bacillus subtilis
YtpA



subtilis]


3072
YtoA [Bacillus subtilis]
UniRef100_O34696
Bacillus subtilis
YtoA


3073



YwoA


3074
Glycosyl transferase, group 1 family
UniRef100_Q6HCB9
Bacillus
TuaC



[Bacillus thuringiensis]

thuringiensis


3075
Asparagine synthetase [glutamine-
UniRef100_P54420
glutamine-
AsnB



hydrolyzing] 1 [Bacillus subtilis]

hydrolyzing


3076
S-adenosylmethionine synthetase
UniRef100_P54419
Bacillus subtilis
MetK



[Bacillus subtilis]


3077
Phosphoenolpyruvate carboxykinase
UniRef100_P54418
ATP
PckA



[ATP] [Bacillus subtilis]


3078
Sodium:dicarboxylate symporter
UniRef100_Q8EP16
Oceanobacillus
DctP



[Oceanobacillus iheyensis]

iheyensis


3079
Hypothetical protein ytmB [Bacillus
UniRef100_O34365
Bacillus subtilis



subtilis]


3080
Putative peptidase [Bacillus subtilis]
UniRef100_O34493
Bacillus subtilis
YtmA


3081
ABC transporter substrate-binding
UniRef100_Q816P5
Bacillus cereus
YtlA



protein [Bacillus cereus]


3082
Putative transporter [Bacillus subtilis]
UniRef100_O34314
Bacillus subtilis
YtlC


3083



YtlD


3084
YtkD [Bacillus subtilis]
UniRef100_O35013
Bacillus subtilis
YtkD










3085
Hypothetical protein [Bacillus
UniRef100_Q6HC91
Bacillus thuringiensis



thuringiensis]











3086
Hypothetical protein ytkC [Bacillus
UniRef100_O34883
Bacillus subtilis
YtkC



subtilis]


3087
General stress protein 20U [Bacillus
UniRef100_P80879
Bacillus subtilis
Dps



subtilis]


3088
Hypothetical protein ytkA [Bacillus
UniRef100_P40768
Bacillus subtilis
YtkA



subtilis]


3089
S-ribosylhomocysteinase [Bacillus
UniRef100_O34667
Bacillus subtilis
LuxS



subtilis]


3090
Hypothetical UPF0161 protein ytjA
UniRef100_O34601
Bacillus subtilis



[Bacillus subtilis]


3091
YtiB [Bacillus subtilis]
UniRef100_O34872
Bacillus subtilis
YtiB


3092
Low-affinity zinc transport protein
UniRef100_Q81F90
Bacillus cereus
YciC



[Bacillus cereus]


3093
High-affinity zinc uptake system protein
UniRef100_Q81EF8
Bacillus cereus
YcdH



znuA [Bacillus cereus]


3094
50S ribosomal protein L31 type B
UniRef100_O34967
Bacillus subtilis



[Bacillus subtilis]


3095
YthA [Bacillus subtilis]
UniRef100_O34655
Bacillus subtilis
YthA


3096
YthB [Bacillus subtilis]
UniRef100_O34505
Bacillus subtilis
YthB


3097
Hypothetical protein [Bacillus cereus]
UniRef100_Q737J1
Bacillus cereus


3098


3099
O-succinylbenzoate synthase (EC
UniRef100_O34514
Bacillus subtilis
MenC



4.2.1.—) (OSB synthase) (OSBS) (4-(2′-



carboxyphenyl)-4-oxybutyric acid



synthase) [Bacillus subtilis]


3100
O-succinylbenzoate--CoA ligase
UniRef100_P23971
Bacillus subtilis
MenE



[Bacillus subtilis]


3101
Naphthoate synthase [Bacillus subtilis]
UniRef100_P23966
Bacillus subtilis
MenB


3102



YtxM


3103
Menaquinone biosynthesis protein
UniRef100_P23970
Includes: 2-
MenD



menD [Includes: 2-succinyl-6-hydroxy-

succinyl-6-



2,4-cyclohexadiene-1-carboxylate

hydroxy-2,4-



synthase (EC 2.5.1.64) (SHCHC

cyclohexadiene-



synthase); 2-oxoglutarate

1-carboxylate



decarboxylase (EC 4.1.1.71) (Alpha-

synthase (EC



ketoglutarate decarboxylase) (KDC)]

2.5.1.64)



[Bacillus subtilis]

(SHCHC





synthase); 2-





oxoglutarate





decarboxylase





(EC 4.1.1.71)





(Alpha-





ketoglutarate





decarboxylase)





(KDC)


3104
Menaquinone-specific isochorismate
UniRef100_P23973
Bacillus subtilis
MenF



synthase [Bacillus subtilis]


3105
Probable 1,4-dihydroxy-2-naphthoate
UniRef100_P39582
Bacillus subtilis
MenA



octaprenyltransferase [Bacillus subtilis]


3106
Hypothetical protein yteA [Bacillus
UniRef100_P42408
Bacillus subtilis
YteA



subtilis]


3107
Glycogen phosphorylase [Bacillus
UniRef100_P39123
Bacillus subtilis
GlgP



subtilis]


3108
Glycogen synthase [Bacillus subtilis]
UniRef100_P39125
Bacillus subtilis
GlgA


3109
Glycogen biosynthesis protein glgD
UniRef100_P39124
Bacillus subtilis
GlgD



[Bacillus subtilis]


3110
Glucose-1-phosphate
UniRef100_P39122
Bacillus subtilis
GlgC



adenylyltransferase [Bacillus subtilis]


3111
1,4-alpha-glucan branching enzyme
UniRef100_P39118
Bacillus subtilis
GlgB



[Bacillus subtilis]


3112



AraR


3113
YuaJ protein [Bacillus subtilis]
UniRef100_O32074
Bacillus subtilis
YuaJ


3114
BH4010 protein [Bacillus halodurans]
UniRef100_Q9K5S8
Bacillus
YhcS





halodurans










3115
BH4011 protein [Bacillus halodurans]
UniRef100_Q9K5S7
Bacillus halodurans


3116











3117



RapD


3118
Pyrrolidone-carboxylate peptidase
UniRef100_P46107
Bacillus
Pcp



[Bacillus amyloliquefaciens]

amyloliquefaciens


3119
BH0597 protein [Bacillus halodurans]
UniRef100_Q9KF88
Bacillus
YuaA





halodurans


3120



YubG


3121



YxxF


3122
YuaE protein [Bacillus subtilis]
UniRef100_O32078
Bacillus subtilis
YuaE


3123
YuaD protein [Bacillus subtilis]
UniRef100_O32079
Bacillus subtilis
YuaD


3124
Alcohol dehydrogenase [Bacillus
UniRef100_P71017
Bacillus subtilis
GbsB



subtilis]


3125
Betaine aldehyde dehydrogenase
UniRef100_P71016
Bacillus subtilis
GbsA



[Bacillus subtilis]


3126
Hypothetical protein yuaC [Bacillus
UniRef100_P71015
Bacillus subtilis
YuaC



subtilis]










3127
UPI00002D3D35 UniRef100 entry
UniRef100_UPI00002D3D35
OpuE











3128
Hypothetical protein yktD [Bacillus
UniRef100_Q45500
Bacillus subtilis
YktD



subtilis]


3129
Alanine racemase 2 [Bacillus subtilis]
UniRef100_P94494
Bacillus subtilis
YncD


3130
Oxalate decarboxylase [Bacillus
UniRef100_Q81GZ6
Bacillus cereus
YoaN



cereus]










3131
Hypothetical protein CAC0135
UniRef100_Q97MQ7
Clostridium acetobutylicum



[Clostridium acetobutylicum]


3132
Hypothetical protein [Bacillus
UniRef100_Q6HGC9
Bacillus thuringiensis



thuringiensis]


3133
Hypothetical protein [Bacillus
UniRef100_Q6HGC8
Bacillus thuringiensis



thuringiensis]


3134


3135
Hypothetical protein [Bacillus
UniRef100_Q6HGC6
Bacillus thuringiensis



thuringiensis]


3136
Hypothetical protein [Bacillus
UniRef100_Q6HGC5
Bacillus thuringiensis



thuringiensis]


3137
Hypothetical conserved protein
UniRef100_Q8ETF5
Oceanobacillus iheyensis



[Oceanobacillus iheyensis]











3138






3139


3140
YkoN [Bacillus subtilis]
UniRef100_O34625
Bacillus subtilis
YkoN


3141
Hypothetical protein ykoP [Bacillus
UniRef100_O34495
Bacillus subtilis
YkoP



subtilis]


3142
Hypothetical UPF0151 protein ykoQ
UniRef100_O35040
Bacillus subtilis
YkoQ



[Bacillus subtilis]


3143
Undecaprenyl-diphosphatase [Bacillus
UniRef100_P94507
Bacillus subtilis
YubB



subtilis]


3144
Hypothetical UPF0118 protein yubA
UniRef100_O32086
Bacillus subtilis
YubA



[Bacillus subtilis]


3145
Hypothetical oxidoreductase yulF
UniRef100_O05265
Bacillus subtilis
YulF



[Bacillus subtilis]


3146
Lmo2256 protein [Listeria
UniRef100_Q929B9
Listeria
YraA



monocytogenes]

monocytogenes


3147



McpA


3148



McpA


3149



McpA


3150
Protein-glutamine gamma-
UniRef100_P40746
Bacillus subtilis
Tgl



glutamyltransferase [Bacillus subtilis]


3151
2-nitropropane dioxygenase [Bacillus
UniRef100_O05413
Bacillus subtilis
YrpB



subtilis]


3152
Hypothetical UPF0047 protein yugU
UniRef100_O05243
Bacillus subtilis
YugU



[Bacillus subtilis]


3153
Hypothetical protein yugT [Bacillus
UniRef100_O05242
Bacillus subtilis
YugT



subtilis]


3154
Transcriptional regulator, TetR family
UniRef100_Q81GX6
Bacillus cereus
YfiR



[Bacillus cereus]


3155
Hypothetical protein yqeB [Bacillus
UniRef100_P54447
Bacillus subtilis
YqeB



subtilis]










3156
Beta(1,4)-glucan glucanohydrolase
UniRef100_Q6D3B7
Erwinia carotovora



[Erwinia carotovora]











3157
Hypothetical UPF0053 protein yugS
UniRef100_O05241
Bacillus subtilis
YugS



[Bacillus subtilis]


3158
Hypothetical protein yugP [Bacillus
UniRef100_O05248
Bacillus subtilis
YugP



subtilis]


3159
YugO protein [Bacillus subtilis]
UniRef100_Q795M8
Bacillus subtilis


3160
Hypothetical protein yugN [Bacillus
UniRef100_O05246
Bacillus subtilis
YugN



subtilis]


3161
Hypothetical protein [Bacillus
UniRef100_Q6HIW1
Bacillus
YdfR



thuringiensis]

thuringiensis


3162
YtaB protein [Bacillus subtilis]
UniRef100_O34694
Bacillus subtilis
YtaB


3163
Predicted acetyltransferase
UniRef100_Q97IT3
Clostridium
YkkB



[Clostridium acetobutylicum]

acetobutylicum


3164
Glucose-6-phosphate isomerase
UniRef100_P80860
Bacillus subtilis
Pgi



[Bacillus subtilis]


3165
Probable NADH-dependent butanol
UniRef100_O05239
Bacillus subtilis
YugJ



dehydrogenase 1 [Bacillus subtilis]


3166
YuzA protein [Bacillus subtilis]
UniRef100_O32087
Bacillus subtilis


3167
General stress protein 13 [Bacillus
UniRef100_P80870
Bacillus subtilis
YugI



subtilis]


3168
Alanine transaminase [Bacillus subtilis]
UniRef100_Q795M6
Bacillus subtilis
AlaT


3169
Transcriptional regulator [Bacillus
UniRef100_O05236
Bacillus subtilis
AlaR



subtilis]


3170
Hypothetical protein yugF [Bacillus
UniRef100_O05235
Bacillus subtilis
YugF



subtilis]


3171
Hypothetical protein yugE [Bacillus
UniRef100_O05234
Bacillus subtilis



subtilis]










3172
Hypothetical protein SMU.305
UniRef100_Q9X669
Streptococcus mutans



[Streptococcus mutans]











3173
Putative aminotransferase B [Bacillus
UniRef100_Q08432
Bacillus subtilis
PatB



subtilis]


3174


3175
Kinase-associated lipoprotein B
UniRef100_Q08429
Bacillus subtilis
KapB



precursor [Bacillus subtilis]


3176
Hypothetical protein yugB [Bacillus
UniRef100_O05231
Bacillus subtilis
KapD



subtilis]


3177



YuxJ


3178



PbpD


3179
Hypothetical protein yuxK [Bacillus
UniRef100_P40761
Bacillus subtilis
YuxK



subtilis]


3180
Hypothetical protein yufK [Bacillus
UniRef100_O05249
Bacillus subtilis
YufK



subtilis]


3181
Hypothetical protein yufL [Bacillus
UniRef100_O05250
Bacillus subtilis
YufL



subtilis]


3182
Hypothetical protein yufM [Bacillus
UniRef100_O05251
Bacillus subtilis
YufM



subtilis]


3183










3184
UPI00003CB938 UniRef100 entry
UniRef100_UPI00003CB938
PssA











3185
Hypothetical protein ybfM [Bacillus
UniRef100_O31453
Bacillus subtilis
YbfM



subtilis]


3186
Phosphatidylserine decarboxylase
UniRef100_Q6HDI5
Bacillus
Psd



[Bacillus thuringiensis]

thuringiensis










3187
UPI00003CC069 UniRef100 entry
UniRef100_UPI00003CC069











3188
IS1627s1-related, transposase
UniRef100_Q7CMD0
Bacillus anthracis str. A2012



[Bacillus anthracis str. A2012]











3189
Na(+)-malate symporter [Bacillus
UniRef100_O05256
Bacillus subtilis
MaeN



subtilis]


3190


3191
Na(+)/H(+) antiporter subunit A
UniRef100_Q9K2S2
Bacillus subtilis
MrpA



[Bacillus subtilis]


3192
Na(+)/H(+) antiporter subunit B
UniRef100_O05259
Bacillus subtilis
MrpB



[Bacillus subtilis]


3193
Na(+)/H(+) antiporter subunit C
UniRef100_O05260
Bacillus subtilis
MrpC



[Bacillus subtilis]


3194
Na(+)/H(+) antiporter subunit D
UniRef100_O05229
Bacillus subtilis
MrpD



[Bacillus subtilis]


3195
Na(+)/H(+) antiporter subunit E
UniRef100_Q7WY60
Bacillus subtilis
MrpE



[Bacillus subtilis]


3196
Na(+)/H(+) antiporter subunit F
UniRef100_O05228
Bacillus subtilis



[Bacillus subtilis]


3197
Na(+)/H(+) antiporter subunit G
UniRef100_O05227
Bacillus subtilis
MrpG



[Bacillus subtilis]


3198



YuxO


3199



ComA


3200



ComP










3201
IS1627s1-related, transposase
UniRef100_Q7CMD0
Bacillus anthracis str. A2012



[Bacillus anthracis str. A2012]










3202
UPI00003CC069 UniRef100 entry
UniRef100_UPI00003CC069












3203



ComP


3204


3205
ComQ [Bacillus subtilis]
UniRef100_Q9K5L3
Bacillus subtilis
ComQ


3206


3207
Hypothetical protein yuzC [Bacillus
UniRef100_O32089
Bacillus subtilis
YuzC



subtilis]


3208
Hypothetical protein yuxH [Bacillus
UniRef100_P14203
Bacillus subtilis
YuxH



subtilis]


3209
YueK protein [Bacillus subtilis]
UniRef100_O32090
Bacillus subtilis
YueK


3210
YueJ protein [Bacillus subtilis]
UniRef100_O32091
Bacillus subtilis
YueJ


3211



YueI


3212


3213
Hypothetical protein yueG [Bacillus
UniRef100_O32094
Bacillus subtilis



subtilis]


3214



YueF


3215
RRF2 family protein [Bacillus cereus]
UniRef100_Q81EX1
Bacillus cereus
YwnA


3216
Probable lipase/esterase
UniRef100_Q7UUA2
Rhodopirellula
YuxL



[Rhodopirellula baltica]

baltica










3217
BH1896 protein [Bacillus halodurans]
UniRef100_Q9KBN0
Bacillus halodurans











3218
YueE protein [Bacillus subtilis]
UniRef100_O32098
Bacillus subtilis
YueE


3219
YueD protein [Bacillus subtilis]
UniRef100_O32099
Bacillus subtilis
YueD


3220
Hypothetical protein yueC [Bacillus
UniRef100_O32100
Bacillus subtilis
YueC



subtilis]


3221
YueB protein [Bacillus subtilis]
UniRef100_O32101
Bacillus subtilis
YueB


3222
YukA protein [Bacillus subtilis]
UniRef100_P71068
Bacillus subtilis
YukA


3223
YukC protein [Bacillus subtilis]
UniRef100_P71070
Bacillus subtilis
YukC


3224
YukD protein [Bacillus subtilis]
UniRef100_P71071
Bacillus subtilis


3225
Lin0049 protein [Listeria innocua]
UniRef100_Q92FQ4
Listeria innocua


3226



YflA


3227
YukF protein [Bacillus subtilis]
UniRef100_P71073
Bacillus subtilis
YukF


3228
Alanine dehydrogenase [Bacillus
UniRef100_Q08352
Bacillus subtilis
Ald



subtilis]


3229


3230
YuiH protein [Bacillus subtilis]
UniRef100_O32103
Bacillus subtilis
YuiH


3231
YuiG protein [Bacillus subtilis]
UniRef100_O32104
Bacillus subtilis
YuiG


3232
YuiF protein [Bacillus subtilis]
UniRef100_O32105
Bacillus subtilis
YuiF


3233
Probable cytosol aminopeptidase
UniRef100_O32106
Bacillus subtilis
YuiE



[Bacillus subtilis]


3234
YuiD protein [Bacillus subtilis]
UniRef100_O32107
Bacillus subtilis
YuiD


3235
YuiC protein [Bacillus subtilis]
UniRef100_O32108
Bacillus subtilis
YuiC


3236
YuiB protein [Bacillus subtilis]
UniRef100_O32109
Bacillus subtilis
YuiB


3237


3238



YumB


3239
Thioredoxine reductase [Bacillus
UniRef100_O05268
Bacillus subtilis
YumC



subtilis]


3240


3241
YdjO protein [Bacillus subtilis]
UniRef100_O34759
Bacillus subtilis


3242



YxbD


3243
Hypothetical protein yutM [Bacillus
UniRef100_O32113
Bacillus subtilis
YutM



subtilis]


3244
Diaminopimelate epimerase [Bacillus
UniRef100_O32114
Bacillus subtilis
DapF



subtilis]


3245
YutK protein [Bacillus subtilis]
UniRef100_O32115
Bacillus subtilis
YutK


3246
YuzB protein [Bacillus subtilis]
UniRef100_O32116
Bacillus subtilis


3247
YutJ protein [Bacillus subtilis]
UniRef100_O32117
Bacillus subtilis
YutJ


3248
YdhG protein [Bacillus subtilis]
UniRef100_O05499
Bacillus subtilis
YdhG


3249
Response regulator aspartate
UniRef100_Q9KBE1
Bacillus
RapI



phosphatase [Bacillus halodurans]

halodurans


3250
Phenolic acid decarboxylase [Bacillus
UniRef100_O07006
Bacillus subtilis
PadC



subtilis]










3251
BH2266 protein [Bacillus halodurans]
UniRef100_Q9KAM1
Bacillus halodurans











3252
YuzD protein [Bacillus subtilis]
UniRef100_O32118
Bacillus subtilis
YuzD


3253
YutI protein [Bacillus subtilis]
UniRef100_O32119
Bacillus subtilis


3254
Probable peptidase yuxL [Bacillus
UniRef100_P39839
Bacillus subtilis
YuxL



subtilis]


3255
Homoserine kinase [Bacillus subtilis]
UniRef100_P04948
Bacillus subtilis
ThrB


3256
Threonine synthase [Bacillus subtilis]
UniRef100_P04990
Bacillus subtilis
ThrC


3257
Homoserine dehydrogenase [Bacillus
UniRef100_P19582
Bacillus subtilis
Hom



subtilis]


3258
Glycerate dehydrogenase
UniRef100_Q8ENW9
Oceanobacillus
YvcT



[Oceanobacillus iheyensis]

iheyensis


3259
YutH protein [Bacillus subtilis]
UniRef100_O32123
Bacillus subtilis
YutH


3260
Hypothetical protein yutG [Bacillus
UniRef100_O32124
Bacillus subtilis
YutG



subtilis]


3261
YutF protein [Bacillus subtilis]
UniRef100_O32125
Bacillus subtilis
YutF


3262
YutE protein [Bacillus subtilis]
UniRef100_O32126
Bacillus subtilis
YutE


3263
YutD protein [Bacillus subtilis]
UniRef100_O32127
Bacillus subtilis


3264
YutC protein [Bacillus subtilis]
UniRef100_O32128
Bacillus subtilis
YutC


3265



LipA


3266
YunA protein [Bacillus subtilis]
UniRef100_O32130
Bacillus subtilis
YunA


3267


3268
Sodium-dependent transporter
UniRef100_Q9K7C5
Bacillus
YocR



[Bacillus halodurans]

halodurans


3269
YunB protein [Bacillus subtilis]
UniRef100_O32131
Bacillus subtilis
YunB


3270
YunC protein [Bacillus subtilis]
UniRef100_O32132
Bacillus subtilis
YunC


3271
YunD protein [Bacillus subtilis]
UniRef100_O32133
Bacillus subtilis
YunD


3272
YunE protein [Bacillus subtilis]
UniRef100_O32134
Bacillus subtilis
YunE


3273
YunF protein [Bacillus subtilis]
UniRef100_O32135
Bacillus subtilis
YunF


3274



YmcC


3275
TetR family transcriptional regulator?
UniRef100_Q67KA4
Symbiobacterium
PksA



[Symbiobacterium thermophilum]

thermophilum


3276


3277
Purine catabolism protein pucG
UniRef100_O32148
Bacillus subtilis
YurG



[Bacillus subtilis]


3278
Allantoate amidohydrolase [Bacillus
UniRef100_O32149
Bacillus subtilis
YurH



subtilis]


3279
Purine catabolism regulatory protein
UniRef100_O32138
Bacillus subtilis
PucR



[Bacillus subtilis]


3280
Multidrug resistance protein B [Bacillus
UniRef100_Q63FH7
Bacillus cereus
Blt



cereus ZK]

ZK


3281
BH2308 protein [Bacillus halodurans]
UniRef100_Q9KAH9
Bacillus
YcgA





halodurans


3282



TrpD










3283
Anthranilate phosphoribosyltransferase
UniRef100_Q8U089
Pyrococcus furiosus



[Pyrococcus furiosus]











3284
Extracellular ribonuclease precursor
UniRef100_O32150
Bacillus subtilis
YurI



[Bacillus subtilis]


3285
BH1977 protein [Bacillus halodurans]
UniRef100_Q9KBF1
Bacillus halodurans


3286



YurR










3287
Putative membrane protein [Bordetella
UniRef100_Q7WGW7
Bordetella bronchiseptica



bronchiseptica]










3288
UPI00003CB453 UniRef100 entry
UniRef100_UPI00003CB453












3289
Response regulator aspartate
UniRef100_P96649
Bacillus subtilis
RapI



phosphatase I [Bacillus subtilis]


3290
YurU protein [Bacillus subtilis]
UniRef100_O32162
Bacillus subtilis
YurU


3291
NifU-like protein [Bacillus subtilis]
UniRef100_O32163
Bacillus subtilis
YurV


3292
Probable cysteine desulfurase [Bacillus
UniRef100_O32164
Bacillus subtilis
Csd



subtilis]


3293
YurX protein [Bacillus subtilis]
UniRef100_O32165
Bacillus subtilis
YurX


3294
Vegetative protein 296 [Bacillus
UniRef100_P80866
Bacillus subtilis
YurY



subtilis]


3295
Lmo2575 protein [Listeria
UniRef100_Q8Y480
Listeria
CzcD



monocytogenes]

monocytogenes


3296


3297
BH3473 protein [Bacillus halodurans]
UniRef100_Q9K796
Bacillus
YurZ





halodurans


3298
YusA protein [Bacillus subtilis]
UniRef100_O32167
Bacillus subtilis
YusA


3299
YusB protein [Bacillus subtilis]
UniRef100_O32168
Bacillus subtilis
YusB


3300
YusC protein [Bacillus subtilis]
UniRef100_O32169
Bacillus subtilis
YusC


3301
Hypothetical protein yusD [Bacillus
UniRef100_O32170
Bacillus subtilis
YusD



subtilis]


3302
YusE protein [Bacillus subtilis]
UniRef100_O32171
Bacillus subtilis


3303
YusF protein [Bacillus subtilis]
UniRef100_O32172
Bacillus subtilis
YusF


3304


3305
Glycine cleavage system H protein
UniRef100_O32174
Bacillus subtilis
GcvH



[Bacillus subtilis]


3306
Hypothetical protein yusI [Bacillus
UniRef100_O32175
Bacillus subtilis
YusI



subtilis]


3307
YusJ protein [Bacillus subtilis]
UniRef100_O32176
Bacillus subtilis
YusJ


3308
YusK protein [Bacillus subtilis]
UniRef100_O32177
Bacillus subtilis
YusK


3309
YusL protein [Bacillus subtilis]
UniRef100_O32178
Bacillus subtilis
YusL


3310


3311


3312
YusN protein [Bacillus subtilis]
UniRef100_O32180
Bacillus subtilis
YusN


3313
Hypothetical protein yusU [Bacillus
UniRef100_O32187
Bacillus subtilis



subtilis]










3314
BH1040 protein [Bacillus halodurans]
UniRef100_Q9KE18
Bacillus halodurans











3315
YusV protein [Bacillus subtilis]
UniRef100_O32188
Bacillus subtilis
YusV


3316



YfhA


3317
YfiZ protein [Bacillus subtilis]
UniRef100_O31568
Bacillus subtilis
YfiZ


3318
YfiY protein [Bacillus subtilis]
UniRef100_O31567
Bacillus subtilis
YfiY


3319
Hypothetical protein yusW precursor
UniRef100_O32189
Bacillus subtilis
YusW



[Bacillus subtilis]


3320
YusX protein [Bacillus subtilis]
UniRef100_O32190
Bacillus subtilis
YusX


3321
D-alanyl-D-alanine carboxypeptidase
UniRef100_Q8ERG0
Oceanobacillus
DacB



[Oceanobacillus iheyensis]

iheyensis


3322
Hypothetical oxidoreductase yusZ
UniRef100_P37959
Bacillus subtilis
YusZ



[Bacillus subtilis]


3323
Metalloregulation DNA-binding stress
UniRef100_P37960
Bacillus subtilis
MrgA



protein [Bacillus subtilis]


3324
Probable serine protease yvtA [Bacillus
UniRef100_Q9R9I1
Bacillus subtilis
YvtA



subtilis]


3325
Transcriptional regulatory protein cssR
UniRef100_O32192
Bacillus subtilis
CssR



[Bacillus subtilis]


3326
Sensor protein cssS [Bacillus subtilis]
UniRef100_O32193
Bacillus subtilis
CssS


3327
YirB [Bacillus subtilis]
UniRef100_O32302
Bacillus subtilis


3328
Putative HTH-type transcriptional
UniRef100_P40950
Bacillus subtilis
YuxN



regulator yuxN [Bacillus subtilis]


3329
Fumarate hydratase class II [Bacillus
UniRef100_P07343
Bacillus subtilis
CitG



subtilis]


3330


3331
Spore germination protein A1 [Bacillus
UniRef100_P07868
Bacillus subtilis
GerAA



subtilis]


3332
Spore germination protein A2 [Bacillus
UniRef100_P07869
Bacillus subtilis
GerAB



subtilis]


3333
Spore germination protein A3 precursor
UniRef100_P07870
Bacillus subtilis
GerAC



[Bacillus subtilis]


3334


3335



YvqC


3336
YvqE protein [Bacillus subtilis]
UniRef100_O32198
Bacillus subtilis
YvqE


3337
YvqF protein [Bacillus subtilis]
UniRef100_O32199
Bacillus subtilis
YvqF


3338
YvqG protein [Bacillus subtilis]
UniRef100_O32200
Bacillus subtilis
YvqG


3339
Hypothetical protein yvqH [Bacillus
UniRef100_O32201
Bacillus subtilis
YvqH



subtilis]


3340
Hypothetical protein yvqI [Bacillus
UniRef100_O32202
Bacillus subtilis
YvqI



subtilis]










3341
Pectate lyase P358 [Bacillus sp. P-358]
UniRef100_Q8RR73
Bacillus sp. P-358











3342
YvqK protein [Bacillus subtilis]
UniRef100_O34899
Bacillus subtilis
YvqK










3343
UPI00002E3648 UniRef100 entry
UniRef100_UPI00002E3648
FabG











3344






3345



GbsB


3346



DapA


3347
Putative metal binding protein, YvrA
UniRef100_O34631
Bacillus subtilis
YvrA



[Bacillus subtilis]


3348
Putative hemin permease, YvrB
UniRef100_O34451
Bacillus subtilis
YvrB



[Bacillus subtilis]


3349
Putative metal binding protein, YvrC
UniRef100_O34805
Bacillus subtilis
YvrC



[Bacillus subtilis]


3350
Transcriptional regulator, GntR family
UniRef100_O815A7
Bacillus
YdhC



[Bacillus anthracis]

anthracis


3351
Putative ketoreductase, YvrD [Bacillus
UniRef100_O34782
Bacillus subtilis
YvrD



subtilis]










3352
UPI00003CC410 UniRef100 entry
UniRef100_UPI00003CC410
YflK











3353
Transcriptional regulators, LysR family
UniRef100_Q81DJ6
Bacillus cereus
AlsR



[Bacillus cereus]


3354
Exo-poly-alpha-D-galacturonosidase,
UniRef100_Q9WYR8
Thermotoga maritima



putative [Thermotoga maritima]


3355
Altronate hydrolase [Bacillus subtilis]
UniRef100_O34673
Bacillus subtilis
UxaA


3356
Altronate oxidoreductase [Bacillus
UniRef100_O34354
Bacillus subtilis
UxaB



subtilis]


3357
Lacl repressor-like protein [Bacillus
UniRef100_Q9JMQ1
Bacillus subtilis
ExuR



subtilis]


3358
Hypothetical symporter yjmB [Bacillus
UniRef100_O34961
Bacillus subtilis
YjmB



subtilis]


3359
Uronate isomerase [Bacillus subtilis]
UniRef100_O34808
Bacillus subtilis
UxaC


3360
Putative sensory protein kinase, YvrG
UniRef100_O34989
Bacillus subtilis
YvrG



[Bacillus subtilis]


3361
Putative DNA binding response
UniRef100_P94504
Bacillus subtilis
YvrH



regulator, YvrH [Bacillus subtilis]


3362
Ferrichrome transport ATP-binding
UniRef100_P49938
Bacillus subtilis
FhuC



protein fhuC [Bacillus subtilis]


3363
Ferrichrome transport system
UniRef100_P49937
Bacillus subtilis
FhuG



permease protein fhuG [Bacillus



subtilis]


3364
Ferrichrome transport system
UniRef100_P49936
Bacillus subtilis
FhuB



permease protein fhuB [Bacillus



subtilis]


3365
Putative arginine ornithine antiporter,
UniRef100_O32204
Bacillus subtilis
YvsH



YvsH [Bacillus subtilis]


3366
Hypothetical protein yvsG precursor
UniRef100_O32205
Bacillus subtilis
YvsG



[Bacillus subtilis]


3367
Putative molybdate binding protein,
UniRef100_O32206
Bacillus subtilis
YvgJ



YvgJ [Bacillus subtilis]


3368



YcnB


3369
Putative reductase protein, YvgN
UniRef100_O32210
Bacillus subtilis
YvgN



[Bacillus subtilis]


3370
Fructokinase [Listeria monocytogenes]
UniRef100_Q722A5
Listeria
YdhR





monocytogenes


3371
Hypothetical protein ycbU precursor
UniRef100_P42253
Bacillus subtilis
YcbU



[Bacillus subtilis]










3372
Hypothetical protein CPE0889
UniRef100_Q8XM01
Clostridium perfringens



[Clostridium perfringens]











3373
YvgS protein [Bacillus subtilis]
UniRef100_O32215
Bacillus subtilis
YvgS


3374
Hypothetical UPF0126 protein yvgT
UniRef100_O32216
Bacillus subtilis
YvgT



[Bacillus subtilis]


3375
Glutamate-rich protein grpB [Bacillus
UniRef100_Q81CT5
Bacillus cereus
YqkA



cereus]


3376
Acetyltransferase, GNAT family
UniRef100_Q6HJN8
Bacillus
YuaI



[Bacillus thuringiensis]

thuringiensis


3377
Disulfide bond formation protein C
UniRef100_O32217
Bacillus subtilis
BdbC



[Bacillus subtilis]


3378
Disulfide bond formation protein D
UniRef100_O32218
Bacillus subtilis
BdbD



precursor [Bacillus subtilis]


3379
YvgW protein [Bacillus subtilis]
UniRef100_O32219
Bacillus subtilis
YvgW


3380
ABC transporter, substrate binding
UniRef100_Q8U7T8
Agrobacterium
YvfK



protein [Agrobacterium tumefaciens]

tumefaciens


3381
Putative sugar transport integral
UniRef100_Q9K442
Streptomyces
YurN



membrane protein [Streptomyces

coelicolor



coelicolor]


3382
Putative sugar transport integral
UniRef100_Q9K441
Streptomyces
YurM



membrane protein [Streptomyces

coelicolor



coelicolor]










3383
Alpha-glucosidase [Clostridium
UniRef100_Q97K36
Clostridium acetobutylicum



acetobutylicum]











3384
Glucan-glucohydrolase [Microbispora
UniRef100_Q59506
Microbispora
YbbD



bispora]

bispora


3385
ABC transporter, ATP-binding protein
UniRef100_Q81NK1
Bacillus
MntB



[Bacillus anthracis]

anthracis


3386
ABC transporter, permease protein
UniRef100_Q81NK2
Bacillus
MntD



[Bacillus anthracis]

anthracis


3387
ABC transporter, manganese-binding
UniRef100_Q71YG9
Listeria
YcdH



protein [Listeria monocytogenes]

monocytogenes


3388
Hypothetical protein [Bacillus cereus]
UniRef100_Q74NQ1
Bacillus cereus
YhjQ


3389
Phosphosugar-binding transcriptional
UniRef100_Q837Y3
Enterococcus
YfiA



regulator, RpiR family, putative

faecalis



[Enterococcus faecalis]


3390
1-phosphofructokinase [Enterococcus
UniRef100_Q837Y2
Enterococcus
FruK



faecalis]

faecalis


3391
PTS system, fructose-specific family,
UniRef100_Q837Y1
Enterococcus
FruA



IIBC components [Enterococcus

faecalis



faecalis]


3392
PTS system, IIA component
UniRef100_Q837Y0
Enterococcus
FruA



[Enterococcus faecalis]

faecalis


3393
Fructoseltagatose bisphosphate
UniRef100_Q7MC78
Vibrio vulnificus
FbaA



aldolase [Vibrio vulnificus]


3394


3395
Heavy metal-transporting ATPase
UniRef100_Q6HF81
Bacillus
YvgX



[Bacillus thuringiensis]

thuringiensis


3396
Copper chaperone copZ [Bacillus
UniRef100_O32221
Bacillus subtilis



subtilis]


3397
YvgZ protein [Bacillus subtilis]
UniRef100_O32222
Bacillus subtilis
YvgZ


3398
Intracellular proteinase [Thermoplasma
UniRef100_Q97BN0
Thermoplasma
YraA



volcanium]

volcanium


3399
Putative HTH-type transcriptional
UniRef100_O05261
Bacillus subtilis
YulB



regulator yulB [Bacillus subtilis]


3400
Rhamnulose kinase/L-fuculose kinase
UniRef100_Q8A1A3
Bacteroides
YulC



[Bacteroides thetaiotaomicron]

thetaiotaomicron










3401
Putative sugar isomerase
UniRef100_Q9XAB3
Streptomyces coelicolor



[Streptomyces coelicolor]


3402
Hypothetical protein [Polyangium
UniRef100_Q9L8B7
Polyangium cellulosum



cellulosum]











3403
Hypothetical oxidoreductase yuxG
UniRef100_P40747
Bacillus subtilis
YuxG



[Bacillus subtilis]


3404
YfhI protein [Bacillus subtilis]
UniRef100_O31577
Bacillus subtilis
YfhI


3405
YvmA [Bacillus subtilis]
UniRef100_O34307
Bacillus subtilis
YvmA


3406
YvmC [Bacillus subtilis]
UniRef100_O34351
Bacillus subtilis
YvmC


3407
Putative cytochrome P450 CYPX
UniRef100_O34926
Bacillus subtilis
CypX



[Bacillus subtilis]










3408
Hypothetical protein [Chromobacterium
UniRef100_Q7NRR6
Chromobacterium violaceum



violaceum]











3409



YvnA


3410
Hypothetical protein BRA0099
UniRef100_Q8FXH8
Brucella suis



[Brucella suis]


3411
Chloroplast Toc64-2 [Physcomitrella
UniRef100_Q6RJN6
Physcomitrella
GatA



patens]

patens


3412
Hypothetical oxidoreductase yvaA
UniRef100_O32223
Bacillus subtilis
YvaA



[Bacillus subtilis]


3413
Putative acyl carrier protein
UniRef100_O32224
Bacillus subtilis
YvaB



phosphodiesterase 2 [Bacillus subtilis]


3414
Hypothetical UPF0176 protein ybfQ
UniRef100_O31457
Bacillus subtilis
YbfQ



[Bacillus subtilis]


3415
Putative DNA binding response
UniRef100_P94504
Bacillus subtilis



regulator, YvrH [Bacillus subtilis]


3416
YvrI protein [Bacillus subtilis]
UniRef100_O34843
Bacillus subtilis
YvrI


3417


3418
Oxalate decarboxylase oxdC [Bacillus
UniRef100_O34714
Bacillus subtilis
OxdC



subtilis]


3419
Hypothetical protein yvrL [Bacillus
UniRef100_O34686
Bacillus subtilis
YvrL



subtilis]










3420
BH3120 protein [Bacillus halodurans]
UniRef100_Q9K886
Bacillus halodurans


3421


3422
Group-specific protein [Bacillus cereus
UniRef100_Q633V1
Bacillus cereus ZK



ZK]


3423


3424


3425










3426
UPI00003CC069 UniRef100 entry
UniRef100_UPI00003CC069











3427
IS1627s1-related, transposase
UniRef100_Q7CMD0
Bacillus anthracis str. A2012



[Bacillus anthracis str. A2012]


3428
LexA repressor [Listeria
UniRef100_Q720B9
Listeria monocytogenes



monocytogenes]


3429
Hypothetical protein [Bacillus cereus
UniRef100_Q630E5
Bacillus cereus ZK



ZK]


3430
Hypothetical protein [Bacillus anthracis]
UniRef100_Q81XX1
Bacillus anthracis


3431


3432
Hypothetical protein RSc1705
UniRef100_Q8XYQ3
Ralstonia solanacearum



[Ralstonia solanacearum]









3433

YxiD










3434
Hypothetical protein [Bacillus
UniRef100_Q6HH72
Bacillus thuringiensis



thuringiensis]











3435



BlyA


3436


3437


3438


3439
YomQ protein [Bacteriophage SPBc2]
UniRef100_O64053
Bacteriophage
YomQ





SPBc2


3440



XkdV


3441


3442


3443



YubE










3444
ORF38 [Bacteriophage phi-105]
UniRef100_Q9ZXE5
Bacteriophage phi-105









3445

YqbO










3446
Hypothetical protein MW1895
UniRef100_Q8NVQ7
Staphylococcus aureus



[Staphylococcus aureus]


3447


3448


3449
Prophage LambdaBa02, major tail
UniRef100_Q81W97
Bacillus anthracis



protein, putative [Bacillus anthracis]


3450


3451
ORF32 [Bacteriophage phi-105]
UniRef100_Q9ZXF1
Bacteriophage phi-105


3452


3453
Gp7 protein [Bacteriophage phi3626]
UniRef100_Q8SBP7
Bacteriophage phi3626


3454
ORF29 [Bacteriophage phi-105]
UniRef100_Q9ZXF4
Bacteriophage phi-105


3455
ORF27 [Bacteriophage phi-105]
UniRef100_Q9ZXF6
Bacteriophage phi-105











3456
ClpP family serine protease, possible
UniRef100_Q97HW4
Clostridium
ClpP



phage related [Clostridium

acetobutylicum



acetobutylicum]










3457
ORF25 [Bacteriophage phi-105]
UniRef100_Q9ZXF8
Bacteriophage phi-105


3458


3459
Putative terminase large subunit
UniRef100_Q8SBQ2
Bacteriophage phi3626



[Bacteriophage phi3626]


3460
Phage terminase-like protein, small
UniRef100_Q97HW1
Clostridium acetobutylicum



subunit [Clostridium acetobutylicum]


3461
Prophage LambdaBa02, HNH
UniRef100_Q81W86
Bacillus anthracis



endonuclease family protein [Bacillus



anthracis]











3462
Spore coat protein D [Bacillus subtilis]
UniRef100_P07791
Bacillus subtilis











3463
ORF16 [Bacteriophage phi-105]
UniRef100_Q9ZXB9
Bacteriophage phi-105


3464


3465


3466
ORF14 [Bacteriophage phi-105]
UniRef100_Q9ZXC1
Bacteriophage phi-105


3467
ORF13 [Bacteriophage phi-105]
UniRef100_Q9ZXC2
Bacteriophage phi-105


3468
ORF12 [Bacteriophage phi-105]
UniRef100_Q9ZXC3
Bacteriophage phi-105


3469


3470
ORF11 [Bacteriophage phi-105]
UniRef100_Q9ZXC4
Bacteriophage phi-105


3471
ORF10 [Bacteriophage phi-105]
UniRef100_Q9ZXC5
Bacteriophage phi-105


3472
DNA, complete sequence
UniRef100_Q9ZXC7
Bacteriophage phi-105



[Bacteriophage phi-105]


3473
DNA, complete sequence
UniRef100_Q9ZXC8
Bacteriophage phi-105



[Bacteriophage phi-105]


3474


3475


3476


3477
Orf 36 [Staphylococcus aureus
UniRef100_Q9T1Z5
Staphylococcus aureus



bacteriophage PVL]

bacteriophage PVL


3478


3479
Orf16 [Bacteriophage bIL312]
UniRef100_Q9AZE4
Bacteriophage bIL312


3480
Gp26 protein [Bacteriophage phi3626]
UniRef100_Q8SBM8
Bacteriophage phi3626


3481
Cl like protein [Lactobacillus casei
UniRef100_O64370
Lactobacillus casei



bacteriophage A2]

bacteriophage A2











3482
Int gene product [Staphylococcus
UniRef100_Q932E5
Staphylococcus
YdcL



aureus]

aureus


3483
SsrA-binding protein [Bacillus subtilis]
UniRef100_O32230
Bacillus subtilis
SmpB


3484
Ribonuclease R [Bacillus subtilis]
UniRef100_O32231
Bacillus subtilis
Rnr


3485
YvaK protein [Bacillus subtilis]
UniRef100_O32232
Bacillus subtilis
YvaK


3486


3487


3488


3489



YvaO


3490



YvaO


3491
Glycine betaine/carnitine/choline
UniRef100_O34742
Bacillus subtilis
OpuCD



transport system permease protein



opuCD [Bacillus subtilis]


3492
Glycine betaine/carnitine/choline-
UniRef100_O32243
Bacillus subtilis
OpuCC



binding protein precursor [Bacillus



subtilis]


3493
Glycine betaine/carnitine/choline
UniRef100_O34878
Bacillus subtilis
OpuCB



transport system permease protein



opuCB [Bacillus subtilis]


3494
Glycine betaine/carnitine/choline
UniRef100_O34992
Bacillus subtilis
OpuCA



transport ATP-binding protein opuCA



[Bacillus subtilis]


3495
ORF-1 [Bacillus subtilis]
UniRef100_O34709
Bacillus subtilis
YvaV


3496



YvbG


3497



YvbI


3498
YvbJ protein [Bacillus subtilis]
UniRef100_O32247
Bacillus subtilis
YvbJ










3499
UPI00003CBDCC UniRef100 entry
UniRef100_UPI00003CBDCC
YfiY











3500






3501
YoaZ [Bacillus subtilis]
UniRef100_O34947
Bacillus subtilis
YoaZ


3502
Enolase [Bacillus subtilis]
UniRef100_P37869
Bacillus subtilis
Eno


3503
2,3-bisphosphoglycerate-independent
UniRef100_P39773
Bacillus subtilis
Pgm



phosphoglycerate mutase [Bacillus



subtilis]


3504
Triosephosphate isomerase [Bacillus
UniRef100_P27876
Bacillus subtilis
TpiA



subtilis]


3505
Phosphoglycerate kinase [Bacillus
UniRef100_P40924
Bacillus subtilis
Pgk



subtilis]


3506
Glyceraldehyde-3-phosphate
UniRef100_P09124
Bacillus subtilis
GapA



dehydrogenase 1 [Bacillus subtilis]


3507
Central glycolytic genes regulator
UniRef100_O32253
Bacillus subtilis
CggR



[Bacillus subtilis]


3508
Hypothetical protein yvbT [Bacillus
UniRef100_O32254
Bacillus subtilis
YvbT



subtilis]


3509
YvbW protein [Bacillus subtilis]
UniRef100_O32257
Bacillus subtilis
YvbW


3510


3511
ABC-type multidrug transport system,
UniRef100_Q8R7K3
Thermoanaerobacter
YfiL



ATPase component

tengcongensis



[Thermoanaerobacter tengcongensis]


3512


3513
YvbY protein [Bacillus subtilis]
UniRef100_O32259
Bacillus subtilis
YvbY


3514
YvfW protein [Bacillus subtilis]
UniRef100_O07021
Bacillus subtilis
YvfW


3515



YvfV


3516
Hypothetical protein yvfI [Bacillus
UniRef100_O07007
Bacillus subtilis
YvfI



subtilis]


3517
Putative L-lactate permease yvfH
UniRef100_P71067
Bacillus subtilis
YvfH



[Bacillus subtilis]


3518
RNA polymerase sigma-54 factor
UniRef100_P24219
Bacillus subtilis
SigL



[Bacillus subtilis]


3519
Hypothetical protein yvfG [Bacillus
UniRef100_P71066
Bacillus subtilis



subtilis]


3520
Hypothetical protein yvfF [Bacillus
UniRef100_P71065
Bacillus subtilis
YvfF



subtilis]


3521
YvfE protein [Bacillus subtilis]
UniRef100_Q795J3
Bacillus subtilis
YvfE


3522
Hypothetical protein yvfD [Bacillus
UniRef100_P71063
Bacillus subtilis
YvfD



subtilis]


3523
Hypothetical protein yvfC [Bacillus
UniRef100_P71062
Bacillus subtilis
YvfC



subtilis]


3524



YvfB


3525



YvfA


3526
Hypothetical protein yveT [Bacillus
UniRef100_P71059
Bacillus subtilis
YveT



subtilis]


3527
Hypothetical protein yveS [Bacillus
UniRef100_P71058
Bacillus subtilis
YveS



subtilis]


3528
Hypothetical protein yveR [Bacillus
UniRef100_P71057
Bacillus subtilis
YveR



subtilis]


3529
Hypothetical protein yveQ [Bacillus
UniRef100_P71056
Bacillus subtilis
YveQ



subtilis]


3530
Hypothetical protein yveP [Bacillus
UniRef100_P71055
Bacillus subtilis
YveP



subtilis]


3531
Hypothetical protein yveO [Bacillus
UniRef100_P71054
Bacillus subtilis
YveO



subtilis]


3532
Hypothetical protein yveN [Bacillus
UniRef100_P71053
Bacillus subtilis
YveN



subtilis]


3533
Hypothetical protein yveM [Bacillus
UniRef100_P71052
Bacillus subtilis
YveM



subtilis]


3534



YveL


3535
Chain length determinant protein
UniRef100_Q72XH7
Bacillus cereus
YveK



[Bacillus cereus]


3536
Hypothetical protein yveJ [Bacillus
UniRef100_P71049
Bacillus subtilis
Slr



subtilis]


3537
General stress protein 14 [Bacillus
UniRef100_P80871
Bacillus subtilis
YwrO



subtilis]


3538



YcbE










3539
UPI000027D43B UniRef100 entry
UniRef100_UPI000027D43B
YvcT










3540
4-hydroxythreonine-4-phosphate
UniRef100_Q8CUU4
Oceanobacillus iheyensis



dehydrogenase (EC 1.1.1.262) (4-



(phosphohydroxy)-L-threonine



dehydrogenase) [Oceanobacillus



iheyensis]


3541
Hypothetical conserved protein
UniRef100_Q8CUU5
Oceanobacillus iheyensis



[Oceanobacillus iheyensis]











3542



GbsB


3543



DapA


3544
Transcriptional regulator
UniRef100_Q8CUU7
Oceanobacillus
RocR



[Oceanobacillus iheyensis]

iheyensis










3545
Hypothetical protein [Bdellovibrio
UniRef100_Q6MH73
Bdellovibrio bacteriovorus



bacteriovorus]











3546
NADH-dependent flavin oxidoreductase
UniRef100_Q8ELB6
Oceanobacillus
YqiG



[Oceanobacillus iheyensis]

iheyensis


3547
Levansucrase precursor [Bacillus
UniRef100_P05655
Bacillus subtilis
SacB



subtilis]


3548
Hypothetical protein yveB [Bacillus
UniRef100_O07003
Bacillus subtilis
YveB



subtilis]










3549
Hypothetical protein [Pseudomonas
UniRef100_Q9I5B4
Pseudomonas aeruginosa



aeruginosa]











3550
ATP-dependent Clp protease
UniRef100_P80244
Bacillus subtilis
ClpP



proteolytic subunit [Bacillus subtilis]


3551
Hypothetical protein yvdD [Bacillus
UniRef100_O06986
Bacillus subtilis
YvdD



subtilis]


3552


3553
Possible metal-dependent
UniRef100_Q6HM73
Bacillus
YdhJ



phosphohydrolase; possible oxetanocin

thuringiensis



A resistance protein [Bacillus



thuringiensis]


3554
Hypothetical protein yvdB [Bacillus
UniRef100_O06984
Bacillus subtilis
YvdB



subtilis]


3555
Hypothetical protein yvdA [Bacillus
UniRef100_O06983
Bacillus subtilis
YvdA



subtilis]


3556
Probable 2-ketogluconate reductase
UniRef100_O32264
Bacillus subtilis
YvcT



[Bacillus subtilis]


3557
BH0392 protein [Bacillus halodurans]
UniRef100_Q9KFT5
Bacillus
YdfE





halodurans


3558
BH0391 protein [Bacillus halodurans]
UniRef100_Q9KFT6
Bacillus
YdfF





halodurans










3559
Ribonuclease [Paenibacillus polymyxa]
UniRef100_Q7M0X3
Paenibacillus polymyxa


3560
Barstar [Bacillus amyloliquefaciens]
UniRef100_P11540
Bacillus amyloliquefaciens











3561
Hypothetical protein yvcN [Bacillus
UniRef100_O06977
Bacillus subtilis
YvcN



subtilis]


3562
HPr-like protein crh [Bacillus subtilis]
UniRef100_O06976
Bacillus subtilis


3563
Hypothetical protein yvcL [Bacillus
UniRef100_O06975
Bacillus subtilis
YvcL



subtilis]


3564
Hypothetical UPF0052 protein yvcK
UniRef100_O06974
Bacillus subtilis
YvcK



[Bacillus subtilis]


3565
Hypothetical UPF0042 protein yvcJ
UniRef100_O06973
Bacillus subtilis
YvcJ



[Bacillus subtilis]


3566
Hypothetical protein yvcI [Bacillus
UniRef100_O06972
Bacillus subtilis
YvcI



subtilis]


3567
Thioredoxin reductase [Bacillus subtilis]
UniRef100_P80880
Bacillus subtilis
TrxB


3568
Hypothetical protein yvcD [Bacillus
UniRef100_O06968
Bacillus subtilis
YvcD



subtilis]


3569
Hypothetical conserved protein
UniRef100_Q8ETL1
Oceanobacillus
YbbJ



[Oceanobacillus iheyensis]

iheyensis


3570
Histidine biosynthesis bifunctional
UniRef100_O34912
Includes:
HisI



protein hisIE [Includes:

Phosphoribosyl-



Phosphoribosyl-AMP cyclohydrolase

AMP



(EC 3.5.4.19) (PRA-CH);

cyclohydrolase



Phosphoribosyl-ATP pyrophosphatase

(EC 3.5.4.19)



(EC 3.6.1.31) (PRA-PH)] [Bacillus

(PRA-CH);



subtilis]

Phosphoribosyl-





ATP





pyrophosphatase





(EC 3.6.1.31)





(PRA-PH)


3571
Imidazole glycerol phosphate synthase
UniRef100_O34727
Bacillus subtilis
HisF



subunit hisF [Bacillus subtilis]


3572
1-(5-phosphoribosyl)-5-[(5-
UniRef100_O35006
(5-
HisA



phosphoribosylamino)methylideneamino]

phosphoribosylamino)



imidazole-4-carboxamide isomerase

methylideneamino



[Bacillus subtilis]


3573
Imidazole glycerol phosphate synthase
UniRef100_O34565
Bacillus subtilis
HisH



subunit hisH [Bacillus subtilis]


3574
Imidazoleglycerol-phosphate
UniRef100_O34683
Bacillus subtilis
HisB



dehydratase [Bacillus subtilis]


3575
Histidinol dehydrogenase [Bacillus
UniRef100_O34651
Bacillus subtilis
HisD



subtilis]


3576
ATP phosphoribosyltransferase
UniRef100_O34520
Bacillus subtilis
HisG



[Bacillus subtilis]


3577



HisZ


3578
YvpB [Bacillus subtilis]
UniRef100_O34735
Bacillus subtilis
YvpB


3579
Pectate lyase Pel-28K [Bacillus sp. P-
UniRef100_Q8L0R5
Bacillus sp. P-
YvpA



2850]

2850


3580
Putative acetyltransferase [Bacillus
UniRef100_O34993
Bacillus subtilis
YvoF



subtilis]


3581
Pyrophosphatase ppaX [Bacillus
UniRef100_Q9JMQ2
Bacillus subtilis
HprP



subtilis]


3582
YvoD [Bacillus subtilis]
UniRef100_O34382
Bacillus subtilis
YvoD


3583
Prolipoprotein diacylglyceryl
UniRef100_O34752
Bacillus subtilis
Lgt



transferase [Bacillus subtilis]


3584
HPr kinase/phosphorylase (EC 2.7.1.—)
UniRef100_O34483
Bacillus subtilis
HprK



(EC 2.7.4.—) (HPrK/P) (HPr(Ser)



kinase/phosphorylase) [Bacillus



subtilis]


3585
YfiV protein [Bacillus subtilis]
UniRef100_O31564
Bacillus subtilis
YfiV


3586
YfiU protein [Bacillus subtilis]
UniRef100_O31563
Bacillus subtilis
YfiU


3587
YvnB [Bacillus subtilis]
UniRef100_O34986
Bacillus subtilis
YvnB


3588
YvlD [Bacillus subtilis]
UniRef100_O34648
Bacillus subtilis
YvlD


3589
YvlC [Bacillus subtilis]
UniRef100_O34719
Bacillus subtilis


3590
YvlB [Bacillus subtilis]
UniRef100_O34628
Bacillus subtilis
YvlB


3591
Hypothetical protein yvlA [Bacillus
UniRef100_O34322
Bacillus subtilis
YvlA



subtilis]


3592
Hypothetical protein yvkN [Bacillus
UniRef100_O34604
Bacillus subtilis



subtilis]


3593


3594
UvrABC system protein A [Bacillus
UniRef100_O34863
Bacillus subtilis
UvrA



subtilis]


3595
UvrABC system protein B [Bacillus
UniRef100_Q659H3
Bacillus
UvrB



amyloliquefaciens]

amyloliquefaciens


3596
CsbA protein [Bacillus subtilis]
UniRef100_P37953
Bacillus subtilis


3597


3598
YvjD [Bacillus subtilis]
UniRef100_O34375
Bacillus subtilis
YvjD


3599



YvzD


3600
Putative protease [Bacillus subtilis]
UniRef100_O35002
Bacillus subtilis
YvjB


3601
Tartrate dehydrogenase
UniRef100_Q8ESH5
Oceanobacillus
YcsA



[Oceanobacillus iheyensis]

iheyensis


3602
Cell wall-binding protein [Bacillus
UniRef100_Q9K6X4
Bacillus
YvcE



halodurans]

halodurans


3603
Cell division protein ftsX homolog
UniRef100_O34876
Bacillus subtilis
FtsX



[Bacillus subtilis]


3604
Cell division ATP-binding protein
UniRef100_O34814
Bacillus subtilis
FtsE



[Bacillus subtilis]


3605
Cytochrome c-551 [Bacillus subtilis]
UniRef100_O34594
Bacillus subtilis
CccB


3606
YvjA [Bacillus subtilis]
UniRef100_O34792
Bacillus subtilis
YvjA


3607
Peptide chain release factor 2 [Bacillus
UniRef100_P28367
Bacillus subtilis
PrfB



subtilis]


3608
Preprotein translocase secA subunit
UniRef100_P28366
Bacillus subtilis
SecA



[Bacillus subtilis]


3609
Hypothetical protein yvyD [Bacillus
UniRef100_P28368
Bacillus subtilis
YvyD



subtilis]


3610


3611
Flagellar protein fliT [Bacillus subtilis]
UniRef100_P39740
Bacillus subtilis
FliT


3612
Flagellar protein fliS [Bacillus subtilis]
UniRef100_P39739
Bacillus subtilis
FliS


3613
Flagellar hook-associated protein 2
UniRef100_P39738
Bacillus subtilis
FliD



[Bacillus subtilis]


3614
Hypothetical protein yvyC [Bacillus
UniRef100_P39737
Bacillus subtilis
YvyC



subtilis]


3615
Flagellin [Bacillus subtilis]
UniRef100_P02968
Bacillus subtilis
Hag


3616
Carbon storage regulator homolog
UniRef100_P33911
Bacillus subtilis



[Bacillus subtilis]


3617
Transmembrane protein [Bacillus
UniRef100_P96503
Bacillus subtilis
YviF



subtilis]


3618
YviE protein [Bacillus subtilis]
UniRef100_P96502
Bacillus subtilis
YviE


3619
Flagellar hook-associated protein 3
UniRef100_P96501
Bacillus subtilis
FlgL



[Bacillus subtilis]


3620
Flagellar hook-associated protein 1
UniRef100_P39810
Bacillus subtilis
FlgK



[Bacillus subtilis]


3621
Hypothetical protein yvyG [Bacillus
UniRef100_P39808
Bacillus subtilis
YvyG



subtilis]


3622
Negative regulator of flagellin synthesis
UniRef100_P39809
Bacillus subtilis



[Bacillus subtilis]


3623
Hypothetical protein yvyF [Bacillus
UniRef100_P39807
Bacillus subtilis
YvyF



subtilis]


3624



ComFC


3625


3626



ComFA


3627
DegV protein [Bacillus subtilis]
UniRef100_P32436
Bacillus subtilis
YviA


3628
Transcriptional regulatory protein degU
UniRef100_P13800
Bacillus subtilis
DegU



[Bacillus subtilis]


3629



DegS


3630
Hypothetical UPF0029 protein yvyE
UniRef100_P32437
Bacillus subtilis
YvyE



[Bacillus subtilis]


3631
Putative transcriptional regulator
UniRef100_P96499
Bacillus subtilis
YvhJ



[Bacillus subtilis]


3632
Hypothetical protein yunG [Bacillus
UniRef100_Q63CU2
Bacillus cereus
YunG



cereus ZK]

ZK


3633
Probable undecaprenyl-phosphate N-
UniRef100_O34753
Bacillus subtilis
TagO



acetylglucosaminyl 1-phosphate



transferase [Bacillus subtilis]


3634
Putative teichuronic acid biosynthesis
UniRef100_O32267
Bacillus subtilis
TuaH



glycosyl transferase tuaH [Bacillus



subtilis]


3635
Putative teichuronic acid biosynthesis
UniRef100_O32268
Bacillus subtilis
TuaG



glycosyl transferase tuaG [Bacillus



subtilis]


3636
Teichuronic acid biosynthesis protein
UniRef100_O32269
Bacillus subtilis
TuaF



tuaF [Bacillus subtilis]


3637
Teichuronic acid biosynthesis protein
UniRef100_O32270
Bacillus subtilis
TuaE



tuaE (Bacillus subtilis]


3638
Putative UDP-glucose 4-epimerase
UniRef100_Q57664
Methanococcus
GalE



[Methanococcus jannaschii]

jannaschii


3639
UDP-glucose 6-dehydrogenase
UniRef100_O32271
Bacillus subtilis
TuaD



[Bacillus subtilis]


3640
Putative teichuronic acid biosynthesis
UniRef100_O32272
Bacillus subtilis
TuaC



glycosyl transferase tuaC [Bacillus



subtilis]


3641
Teichuronic acid biosynthesis protein
UniRef100_O32273
Bacillus subtilis
TuaB



tuaB [Bacillus subtilis]










3642
UPI00003CB85E UniRef100 entry
UniRef100_UPI00003CB85E
TuaA











3643



LytC


3644
Amidase enhancer precursor [Bacillus
UniRef100_Q02113
Bacillus subtilis
LytB



subtilis]


3645



LytA


3646
Membrane-bound protein lytR [Bacillus
UniRef100_Q02115
Bacillus subtilis
LytR



subtilis]


3647
UDP-N-acetylglucosamine 2-
UniRef100_P39131
Bacillus subtilis
YvyH



epimerase [Bacillus subtilis]


3648
UTP--glucose-1-phosphate
UniRef100_Q05852
Bacillus subtilis
GtaB



uridylyltransferase [Bacillus subtilis]


3649



TagH


3650
Teichoic acid translocation permease
UniRef100_P42953
Bacillus subtilis
TagG



protein tagG [Bacillus subtilis]


3651
Teichoic acid biosynthesis protein
UniRef100_Q9CH20
Lactococcus
TagF



[Lactococcus lactis]

lactis


3652
CDP-glycerol: poly(glycerophosphate)
UniRef100_P13485
Bacillus subtilis
TagF



glycerophosphotransferase [Bacillus



subtilis]


3653



TagD


3654



TagA


3655
Putative CDP-
UniRef100_P27621
Bacillus subtilis
TagB



glycerol: glycerophosphate



glycerophosphotransferase [Bacillus



subtilis]


3656
Beta-N-acetylglucosaminidase
UniRef100_P39848
Bacillus subtilis
LytD



precursor [Bacillus subtilis]


3657
Mannose-6-phosphate isomerase
UniRef100_P39841
Bacillus subtilis
Pmi



[Bacillus subtilis]


3658


3659
Anaerobic ribonucleoside-triphosphate
UniRef100_Q733N6
Bacillus cereus



reductase, putative [Bacillus cereus]


3660
2-keto-3-deoxygluconate permease
UniRef100_P50847
Bacillus subtilis
KdgT



[Bacillus subtilis]


3661
KHG/KDPG aldolase [Includes: 4-
UniRef100_P50846
Includes: 4-
KdgA



hydroxy-2-oxoglutarate aldolase (EC

hydroxy-2-



4.1.3.16) (2-keto-4-hydroxyglutarate

oxoglutarate



aldolase) (KHG-aldolase); 2-dehydro-

aldolase (EC



3-deoxy-phosphogluconate aldolase

4.1.3.16) (2-keto-



(EC 4.1.2.14) (Phospho-2-dehydro-3-

4-



deoxygluconate aldolase) (Phospho-2-

hydroxyglutarate



keto-3-d

aldolase) (KHG-





aldolase); 2-





dehydro-3-





deoxy-





phosphogluconate





aldolase (EC





4.1.2.14)





(Phospho-2-





dehydro-3-





deoxygluconate





aldolase)





(Phospho-2-





keto-3-





deoxygluconate





aldol


3662
2-dehydro-3-deoxygluconokinase
UniRef100_P50845
Bacillus subtilis
KdgK



[Bacillus subtilis]


3663
HTH-type transcriptional regulator
UniRef100_P50844
Bacillus subtilis
KdgR



kdgR [Bacillus subtilis]


3664



KduI


3665
2-deoxy-D-gluconate 3-dehydrogenase
UniRef100_P50842
Bacillus subtilis
KduD



[Bacillus subtilis]


3666
Alcohol dehydrogenase, glutathione-
UniRef100_Q6HGX1
Bacillus
AdhB



dependent formaldehyde

thuringiensis



dehydrogenase [Bacillus thuringiensis]


3667
YwtG protein [Bacillus subtilis]
UniRef100_P96742
Bacillus subtilis
YwtG


3668
Hypothetical transcriptional regulator
UniRef100_Q7WY78
Bacillus subtilis
YwtF



ywtF [Bacillus subtilis]


3669
YwtE protein [Bacillus subtilis]
UniRef100_P96741
Bacillus subtilis
YwtE


3670
Gamma-DL-glutamyl hydrolase
UniRef100_P96740
Bacillus subtilis
YwtD



precursor [Bacillus subtilis]


3671


3672
YwtB protein [Bacillus subtilis]
UniRef100_P96738
Bacillus subtilis
YwtB


3673
YwtA protein [Bacillus subtilis]
UniRef100_P96737
Bacillus subtilis
YwtA


3674
YwsC protein [Bacillus subtilis]
UniRef100_P96736
Bacillus subtilis
YwsC


3675



RbsR


3676
Ribokinase [Bacillus subtilis]
UniRef100_P36945
Bacillus subtilis
RbsK


3677
High affinity ribose transport protein
UniRef100_P36946
Bacillus subtilis
RbsD



rbsD [Bacillus subtilis]


3678
Ribose transport ATP-binding protein
UniRef100_P36947
Bacillus subtilis
RbsA



rbsA [Bacillus subtilis]


3679
Ribose transport system permease
UniRef100_P36948
Bacillus subtilis
RbsC



protein rbsC [Bacillus subtilis]


3680
D-ribose-binding protein precursor
UniRef100_P36949
Bacillus subtilis
RbsB



[Bacillus subtilis]


3681
Alpha-acetolactate decarboxylase
UniRef100_Q04777
Bacillus subtilis
AlsD



[Bacillus subtilis]


3682
Acetolactate synthase [Bacillus subtilis]
UniRef100_Q04789
Bacillus subtilis
AlsS


3683
HTH-type transcriptional regulator alsR
UniRef100_Q04778
Bacillus subtilis
AlsR



[Bacillus subtilis]


3684
YwrD protein [Bacillus subtilis]
UniRef100_O05218
Bacillus subtilis
YwrD


3685
YwrC protein [Bacillus subtilis]
UniRef100_O05217
Bacillus subtilis
YwrC


3686
YwrB protein [Bacillus subtilis]
UniRef100_O05216
Bacillus subtilis
YwrB


3687
YwrA protein [Bacillus subtilis]
UniRef100_O05215
Bacillus subtilis
YwrA


3688
Putative protein-tyrosine phosphatase
UniRef100_P96717
Bacillus subtilis
YwqE



ywqE [Bacillus subtilis]


3689
Hypothetical protein ywqC [Bacillus
UniRef100_P96715
Bacillus subtilis
YwqC



subtilis]


3690
Hypothetical protein ywqB [Bacillus
UniRef100_P96714
Bacillus subtilis
YwqB



subtilis]


3691
YwqA protein [Bacillus subtilis]
UniRef100_P94593
Bacillus subtilis
YwqA


3692
YwpJ protein [Bacillus subtilis]
UniRef100_P94592
Bacillus subtilis
YwpJ


3693
YwpI protein [Bacillus subtilis]
UniRef100_P94591
Bacillus subtilis
GlcR


3694
Fructose-6-phosphate aldolase 2
UniRef100_P58424
Escherichia coli
YwjH



[Escherichia coli O157:H7]

O157:H7


3695
Hypothetical protein PM1968
UniRef100_Q9CJM9
Pasteurella
FabG



[Pasteurella multocida]

multocida










3696
PTS system, sorbitol-specific IIA
UniRef100_Q82YX6
Enterococcus faecalis



component [Enterococcus faecalis]


3697
PTS system, sorbitol-specific IIBC
UniRef100_Q82YX5
Enterococcus faecalis



components [Enterococcus faecalis]









3698
UPI00002F4A3A UniRef100 entry
UniRef100_UPI00002F4A3A










3699
Sorbitol operon activator
UniRef100_Q9X673
Streptococcus mutans



[Streptococcus mutans]











3700
Putative transcriptional regulator of
UniRef100_Q83PA8
Shigella flexneri
DeoR



sorbose uptake and utilization genes



[Shigella flexneri]


3701
YwpH protein [Bacillus subtilis]
UniRef100_P94590
Bacillus subtilis
YwpH


3702
Hypothetical protein ywpG [Bacillus
UniRef100_P94589
Bacillus subtilis
YwpG



subtilis]


3703
YwpF protein [Bacillus subtilis]
UniRef100_P94588
Bacillus subtilis
YwpF


3704
Large-conductance mechanosensitive
UniRef100_P94585
Bacillus subtilis
MscL



channel [Bacillus subtilis]


3705
(3R)-hydroxymyristoyl-[acyl carrier
UniRef100_Q81JE0
acyl carrier
YwpB



protein] dehydratase (EC 4.2.1.—) ((3R)-

protein



hydroxymyristoyl ACP dehydrase)



[Bacillus anthracis]


3706
Flagellar hook-basal body complex
UniRef100_P39753
Bacillus subtilis
FlhP



protein flhP [Bacillus subtilis]


3707
Flagellar hook-basal body complex
UniRef100_P39752
Bacillus subtilis
FlhO



protein flhO [Bacillus subtilis]


3708
MreB-like protein [Bacillus subtilis]
UniRef100_P39751
Bacillus subtilis
MbI


3709
Stage III sporulation protein D [Bacillus
UniRef100_P15281
Bacillus subtilis



subtilis]


3710


3711
Hypothetical protein ywoH [Bacillus
UniRef100_P94578
Bacillus subtilis
YwoH



subtilis]


3712
Hypothetical protein ywoG [Bacillus
UniRef100_P94577
Bacillus subtilis
YwoG



subtilis]


3713
Hypothetical protein ywoA [Bacillus
UniRef100_P94571
Bacillus subtilis
YwoA



subtilis]


3714
Manganese-dependent inorganic
UniRef100_Q63AC7
Bacillus cereus
PpaC



pyrophosphatase [Bacillus cereus ZK]

ZK


3715
Probable manganese catalase
UniRef100_P80878
Bacillus subtilis
YdbD



[Bacillus subtilis]


3716
Hypothetical protein yqjF [Bacillus
UniRef100_P54543
Bacillus subtilis
YqjF



subtilis]


3717
BH2605 protein [Bacillus halodurans]
UniRef100_Q9K9P0
Bacillus
YhcV





halodurans


3718
Hypothetical protein yqxL [Bacillus
UniRef100_P40948
Bacillus subtilis
YqxL



subtilis]


3719
Hypothetical UPF0053 protein yqhB
UniRef100_P54505
Bacillus subtilis
YqhB



[Bacillus subtilis]


3720
Ammonium transporter nrgA [Bacillus
UniRef100_Q07429
Bacillus subtilis
NrgA



subtilis]


3721
Nitrogen regulatory PII-like protein
UniRef100_Q07428
Bacillus subtilis
NrgB



[Bacillus subtilis]


3722
ATP-dependent Clp protease
UniRef100_Q81CH1
Bacillus cereus
ClpP



proteolytic subunit [Bacillus cereus]


3723
RNA polymerase ECF-type sigma
UniRef100_Q63AF9
Bacillus cereus
SigM



factor [Bacillus cereus ZK]

ZK


3724
Hypothetical protein ywnI [Bacillus
UniRef100_P71044
Bacillus subtilis
SpollQ



subtilis]


3725
Hypothetical protein ywnH [Bacillus
UniRef100_P71043
Bacillus subtilis
YwnH



subtilis]


3726
Hypothetical protein ywnF [Bacillus
UniRef100_P71041
Bacillus subtilis
YwnF



subtilis]


3727
Probable cardiolipin synthetase 2
UniRef100_P71040
Bacillus subtilis
YwnE



[Bacillus subtilis]










3728
UPI00003CB764 UniRef100 entry
UniRef100_UPI00003CB764
YhcA











3729
Antibiotic/siderophore biosynthesis
UniRef100_Q81DP7
Bacillus cereus




protein [Bacillus cereus]


3730
Probable serine activating enzyme
UniRef100_P45745
Bacillus subtilis
DhbF



[Bacillus subtilis]


3731
Isochorismatase [Bacillus subtilis]
UniRef100_P45743
Bacillus subtilis
DhbB


3732
2,3-dihydroxybenzoate-AMP ligase
UniRef100_P40871
Bacillus subtilis
DhbE



[Bacillus subtilis]


3733
Isochorismate synthase dhbC [Bacillus
UniRef100_P45744
Bacillus subtilis
DhbC



subtilis]


3734
2,3-dihydro-2,3-dihydroxybenzoate
UniRef100_P39071
Bacillus subtilis
DhbA



dehydrogenase [Bacillus subtilis]


3735
YuiI protein [Bacillus subtilis]
UniRef100_O32102
Bacillus subtilis
YuiI


3736
Iron-uptake system permease protein
UniRef100_P40411
Bacillus subtilis
FeuC



feuC [Bacillus subtilis]


3737
Iron-uptake system permease protein
UniRef100_P40410
Bacillus subtilis
FeuB



feuB [Bacillus subtilis]


3738
Iron-uptake system binding protein
UniRef100_P40409
Bacillus subtilis
FeuA



precursor [Bacillus subtilis]


3739
Putative HTH-type transcriptional
UniRef100_P40408
Bacillus subtilis
YbbB



regulator ybbB [Bacillus subtilis]


3740
Hypothetical protein ywnG [Bacillus
UniRef100_P71042
Bacillus subtilis
YwnC



subtilis]










3741
UPI00003CC069 UniRef100 entry
UniRef100_UPI00003CC069











3742
IS1627s1-related, transposase
UniRef100_Q7CMD0
Bacillus anthracis str. A2012



[Bacillus anthracis str. A2012]











3743
Hypothetical protein ywnB [Bacillus
UniRef100_P71037
Bacillus subtilis
YwnB



subtilis]


3744
Hypothetical protein ywnA [Bacillus
UniRef100_P71036
Bacillus subtilis
YwnA



subtilis]


3745


3746
Hypothetical protein ywmF [Bacillus
UniRef100_P70963
Bacillus subtilis
YwmF



subtilis]


3747



MoaA


3748
FdhD protein homolog [Bacillus
UniRef100_P39756
Bacillus subtilis
FdhD



subtilis]


3749
BH2528 protein [Bacillus halodurans]
UniRef100_Q9K9W7
Bacillus
YbfB





halodurans


3750
Formate dehydrogenase alpha chain
UniRef100_Q9K9W5
Bacillus
YjgC



[Bacillus halodurans]

halodurans


3751



YwmD


3752
Hypothetical protein ywmC precursor
UniRef100_P70960
Bacillus subtilis
YwmC



[Bacillus subtilis]


3753
Stage II sporulation protein D [Bacillus
UniRef100_P07372
Bacillus subtilis
SpoIID



subtilis]


3754
UDP-N-acetylglucosamine 1-
UniRef100_P70965
Bacillus subtilis
MurAA



carboxyvinyltransferase 1 [Bacillus



subtilis]


3755
YwmB protein [Bacillus subtilis]
UniRef100_O32277
Bacillus subtilis
YwmB


3756
YwzB protein [Bacillus subtilis]
UniRef100_O32278
Bacillus subtilis


3757
ATP synthase epsilon chain [Bacillus
UniRef100_P37812
Bacillus subtilis
AtpC



subtilis]


3758
ATP synthase beta chain [Bacillus
UniRef100_P37809
Bacillus subtilis
AtpD



subtilis]


3759
ATP synthase gamma chain [Bacillus
UniRef100_P37810
Bacillus subtilis
AtpG



subtilis]


3760
ATP synthase alpha chain [Bacillus
UniRef100_Q659H2
Bacillus
AtpA



amyloliquefaciens]

amyloliquefaciens


3761
ATP synthase delta chain [Bacillus
UniRef100_P37811
Bacillus subtilis
AtpH



subtilis]


3762
ATP synthase B chain [Bacillus subtilis]
UniRef100_P37814
Bacillus subtilis
AtpF


3763
ATP synthase a chain [Bacillus subtilis]
UniRef100_P37813
Bacillus subtilis
AtpB


3764



AtpI


3765
Uracil phosphoribosyltransferase
UniRef100_P39149
Bacillus subtilis
Upp



[Bacillus subtilis]


3766
Serine hydroxymethyltransferase
UniRef100_P39148
Bacillus subtilis
GlyA



[Bacillus subtilis]


3767
Hypothetical protein ywlG [Bacillus
UniRef100_P39157
Bacillus subtilis
YwlG



subtilis]


3768
Hypothetical lacA/rpiB family protein
UniRef100_P39156
Bacillus subtilis
YwlF



ywlF [Bacillus subtilis]


3769
Putative low molecular weight protein-
UniRef100_P39155
Bacillus subtilis
YwlE



tyrosine-phosphatase ywlE [Bacillus



subtilis]


3770
Hypothetical UPF0059 protein ywlD
UniRef100_P39154
Bacillus subtilis
YwlD



[Bacillus subtilis]


3771
Hypothetical protein ywlC [Bacillus
UniRef100_P39153
Bacillus subtilis
YwlC



subtilis]


3772
Hypothetical protein ywlB [Bacillus
UniRef100_P39152
Bacillus subtilis
YwlB



subtilis]


3773
Stage II sporulation protein R [Bacillus
UniRef100_P39151
Bacillus subtilis
SpoIIR



subtilis]


3774
Hypothetical protein yhjl [Bacillus
UniRef100_O07563
Bacillus subtilis
GlcP



subtilis]


3775
Hypothetical protein yhjJ [Bacillus
UniRef100_O07564
Bacillus subtilis
YhjJ



subtilis]


3776
Hypothetical protein [Bacillus anthracis]
UniRef100_Q81NC8
Bacillus
YhjK





anthracis


3777
Hypothetical protein yhjL [Bacillus
UniRef100_O07566
Bacillus subtilis
YhjL



subtilis]


3778
Hypothetical protein yhjM [Bacillus
UniRef100_O07567
Bacillus subtilis
YhjM



subtilis]


3779
HemK protein homolog [Bacillus
UniRef100_P45873
Bacillus subtilis
YwkE



subtilis]


3780
Peptide chain release factor 1 [Bacillus
UniRef100_P45872
Bacillus subtilis
PrfA



subtilis]


3781
Hypothetical protein ywkD [Bacillus
UniRef100_P45871
Bacillus subtilis
YwkD



subtilis]


3782
Hypothetical protein ywkC [Bacillus
UniRef100_P45870
Bacillus subtilis
YwkC



subtilis]


3783
Hypothetical protein ywkB [Bacillus
UniRef100_P45869
Bacillus subtilis
YwkB



subtilis]


3784
Probable NAD-dependent malic
UniRef100_P45868
Bacillus subtilis
YwkA



enzyme 2 [Bacillus subtilis]


3785
Thymidine kinase [Bacillus subtilis]
UniRef100_Q03221
Bacillus subtilis
Tdk


3786
50S ribosomal protein L31 [Bacillus
UniRef100_Q03223
Bacillus subtilis



subtilis]


3787
Transcription termination factor rho
UniRef100_Q03222
Bacillus subtilis
Rho



[Bacillus subtilis]


3788
Hypothetical protein ywjI [Bacillus
UniRef100_Q03224
Bacillus subtilis
YwjI



subtilis]


3789
UDP-N-acetylglucosamine 1-
UniRef100_P19670
Bacillus subtilis
MurAB



carboxyvinyltransferase 2 [Bacillus



subtilis]


3790
Transaldolase [Bacillus subtilis]
UniRef100_P19669
Bacillus subtilis
YwjH


3791
Probable fructose-bisphosphate
UniRef100_P13243
Bacillus subtilis
FbaA



aldolase 1 [Bacillus subtilis]


3792
Sporulation initiation
UniRef100_P06628
Bacillus subtilis
Spo0F



phosphotransferase F [Bacillus subtilis]


3793
Hypothetical protein ywjG [Bacillus
UniRef100_P06629
Bacillus subtilis
YwjG



subtilis]


3794



PyrG


3795
DNA-directed RNA polymerase delta
UniRef100_P12464
Bacillus subtilis
RpoE



subunit [Bacillus subtilis]


3796
Acyl-CoA dehydrogenase [Bacillus
UniRef100_Q814S9
Bacillus cereus
AcdA



cereus]


3797
Acyl-CoA dehydrogenase [Bacillus
UniRef100_Q81JV7
Bacillus
MmgC



anthracis]

anthracis










3798
UPI00003CC303 UniRef100 entry
UniRef100_UPI00003CC303
MmgB











3799
Acetyl-CoA acetyltransferase
UniRef100_Q8EM47
Oceanobacillus
MmgA



[Oceanobacillus iheyensis]

iheyensis


3800
Hypothetical protein ywjF [Bacillus
UniRef100_P45866
Bacillus subtilis
YwjF



subtilis]


3801



YwjE


3802
Hypothetical protein ywjC [Bacillus
UniRef100_P45863
Bacillus subtilis



subtilis]


3803
Arginyl-tRNA synthetase [Bacillus
UniRef100_P46906
Bacillus subtilis
ArgS



subtilis]


3804



YwlB


3805
Agmatinase [Bacillus subtilis]
UniRef100_P70999
Bacillus subtilis
SpeB


3806
Spermidine synthase [Bacillus subtilis]
UniRef100_P70998
Bacillus subtilis
SpeE










3807
UPI00002A1DD1 UniRef100 entry
UniRef100_UPI00002A1DD1












3808
Hypothetical protein ywhE [Bacillus
UniRef100_P70997
Bacillus subtilis
YwhE



subtilis]


3809
Hypothetical protein ywhD [Bacillus
UniRef100_P70996
Bacillus subtilis
YwhD



subtilis]


3810
Hypothetical protein ywhC [Bacillus
UniRef100_P70995
Bacillus subtilis
YwhC



subtilis]


3811
Probable tautomerase ywhB [Bacillus
UniRef100_P70994
Bacillus subtilis



subtilis]










3812
Conserved protein [Methanosarcina
UniRef100_Q8PUC6
Methanosarcina mazei



mazei]











3813
Hypothetical protein ywgA [Bacillus
UniRef100_P71046
Bacillus subtilis
YwgA



subtilis]


3814
Hypothetical protein ywfO [Bacillus
UniRef100_P39651
Bacillus subtilis
YwfO



subtilis]


3815
YwzC protein [Bacillus subtilis]
UniRef100_O32280
Bacillus subtilis


3816
Prespore specific transcriptional
UniRef100_P39650
Bacillus subtilis
RsfA



regulator rsfA [Bacillus subtilis]


3817
Hypothetical protein yjdJ precursor
UniRef100_O31651
Bacillus subtilis



[Bacillus subtilis]


3818
Hypothetical protein ywfL [Bacillus
UniRef100_P39648
Bacillus subtilis
YwfL



subtilis]


3819
4-hydroxybenzoate 3-monooxygenase
UniRef100_Q59724
Pseudomonas
YhjG



[Pseudomonas sp]

sp


3820
Benzoate transporter protein
UniRef100_Q6XUN5
Pseudomonas
YceI



[Pseudomonas sp. ND6]

sp. ND6










3821
Putative 3,4-dihydroxyphenylacetate
UniRef100_Q6N986
Rhodopseudomonas palustris



2,3-dioxygenase [Rhodopseudomonas



palustris]


3822
4-oxalocrotonate tautomerase
UniRef100_Q9Z431
Pseudomonas putida



[Pseudomonas putida]


3823
4-oxalocrotonate decarboxylase
UniRef100_Q84I96
Pseudomonas sp. S-47



[Pseudomonas sp. S-47]











3824



DhaS


3825
Putative transcriptional regulator
UniRef100_Q9F7C7
Agrobacterium
KipR



[Agrobacterium tumefaciens]

tumefaciens










3826
Hypothetical protein SCO6305
UniRef100_Q93RT6
Streptomyces coelicolor



[Streptomyces coelicolor]











3827
Phosphate acetyltransferase [Bacillus
UniRef100_P39646
Bacillus subtilis
Pta



subtilis]


3828
Hypothetical protein ywfI [Bacillus
UniRef100_P39645
Bacillus subtilis
YwfI



subtilis]


3829



YuaB


3830
Na+-transporting ATP synthase
UniRef100_Q9KF87
Bacillus
YubG



[Bacillus halodurans]

halodurans


3831
Hypothetical protein ywdL [Bacillus
UniRef100_P39620
Bacillus subtilis
YwdL



subtilis]


3832
Multidrug ABC transporter, permease
UniRef100_Q63D13
Bacillus cereus
YfiN



[Bacillus cereus ZK]

ZK


3833
ABC transporter, permease protein
UniRef100_Q73AB4
Bacillus cereus
YfiM



[Bacillus cereus]


3834
Multidrug ABC transporter, ATP-
UniRef100_Q63D15
Bacillus cereus
YfiL



binding protein [Bacillus cereus ZK]

ZK


3835
Sensor histidine kinase, putative
UniRef100_Q73AB6
Bacillus cereus
YfiJ



[Bacillus cereus]


3836
DNA-binding response regulator, LuxR
UniRef100_Q73AB7
Bacillus cereus
YfiK



family [Bacillus cereus]


3837



YwdK


3838
Putative purine permease ywdJ
UniRef100_P39618
Bacillus subtilis
YwdJ



[Bacillus subtilis]


3839
Hypothetical protein ywdI [Bacillus
UniRef100_P39617
Bacillus subtilis
YwdI



subtilis]


3840
Probable aldehyde dehydrogenase
UniRef100_P39616
Bacillus subtilis
YwdH



ywdH [Bacillus subtilis]


3841
Uracil-DNA glycosylase [Bacillus
UniRef100_P39615
Bacillus subtilis
Ung



subtilis]


3842



YwdF


3843
Phosphomethylpyrimidine kinase
UniRef100_P39610
Bacillus subtilis
ThiD



[Bacillus subtilis]


3844
Hypothetical protein ywdA [Bacillus
UniRef100_P39609
Bacillus subtilis



subtilis]


3845
Sucrose-6-phosphate hydrolase
UniRef100_P07819
Bacillus subtilis
SacA



[Bacillus subtilis]


3846
PTS system, sucrose-specific IIBC
UniRef100_P05306
Bacillus subtilis
SacP



component [Bacillus subtilis]


3847



SacT


3848


3849
Minor extracellular protease vpr
UniRef100_P29141
Bacillus subtilis
Vpr



precursor [Bacillus subtilis]


3850


3851



YwcH


3852
Nitro/flavin reductase [Bacillus subtilis]
UniRef100_P39605
Bacillus subtilis
NfrA


3853
N-acyl-L-amino acid amidohydrolase
UniRef100_Q9KCF8
Bacillus
YhaA



[Bacillus halodurans]

halodurans


3854
Hypothetical protein ywcF [Bacillus
UniRef100_P39604
Bacillus subtilis
RodA



subtilis]


3855



RodA


3856
Membrane protein [Clostridium tetani]
UniRef100_Q897A1
Clostridium





tetani


3857
Hypothetical protein ywcE precursor
UniRef100_P39603
Bacillus subtilis



[Bacillus subtilis]










3858
Clostripain [Methanosarcina
UniRef100_Q8TIY6
Methanosarcina acetivorans



acetivorans]











3859
YfmK protein [Bacillus subtilis]
UniRef100_Q34536
Bacillus subtilis
YfmK


3860
ABC transporter, ATP-
UniRef100_Q882Y2
Pseudomonas
YgaD



binding/permease protein

syringae



[Pseudomonas syringae]


3861
HlyB/MsbA family ABC transporter
UniRef100_Q7NIB9
Gloeobacter
YwjA



[Gloeobacter violaceus]

violaceus


3862


3863


3864


3865


3866
Response regulator [Bacillus cereus
UniRef100_Q63FE4
Bacillus cereus
ComA



ZK]

ZK


3867


3868
Quinol oxidase polypeptide IV [Bacillus
UniRef100_P34959
Bacillus subtilis
QoxD



subtilis]


3869
Quinol oxidase polypeptide III (EC
UniRef100_P34958
Bacillus subtilis
QoxC



1.10.3.—) (Quinol oxidase aa3-600,



subunit qoxC) (Oxidase aa(3)-600



subunit 3) [Bacillus subtilis]


3870
Quinol oxidase polypeptide I (EC
UniRef100_P34956
Bacillus subtilis
QoxB



1.10.3.—) (Quinol oxidase aa3-600,



subunit qoxB) (Oxidase aa(3)-600



subunit 1) [Bacillus subtilis]


3871



QoxA


3872
UDP-glucose 4-epimerase
UniRef100_Q8R8R8
Thermoanaerobacter
GalE



[Thermoanaerobacter tengcongensis]

tengcongensis


3873


3874


3875


3876
Hypothetical protein ywqN [Bacillus
UniRef100_P96726
Bacillus subtilis
YwqN



subtilis]


3877
Hypothetical protein ywcB [Bacillus
UniRef100_P39600
Bacillus subtilis



subtilis]


3878
Putative symporter ywcA [Bacillus
UniRef100_P39599
Bacillus subtilis
YwcA



subtilis]


3879



XylB


3880
Xylose isomerase [Bacillus sp.]
UniRef100_P54272
Bacillus sp.
XylA


3881



XylR


3882
Thiamine-phosphate
UniRef100_P39594
Bacillus subtilis
ThiE



pyrophosphorylase [Bacillus subtilis]


3883
Hydroxyethylthiazole kinase [Bacillus
UniRef100_P39593
Bacillus subtilis
ThiM



subtilis]


3884
Putative HTH-type transcriptional
UniRef100_P39592
Bacillus subtilis
YwbI



regulator ywbI [Bacillus subtilis]


3885
Putative HTH-type transcriptional
UniRef100_P39592
Bacillus subtilis
YwbI



regulator ywbI [Bacillus subtilis]


3886


3887



YwqH


3888
Holin-like protein cidA [Bacillus subtilis]
UniRef100_P39591
Bacillus subtilis
YwbH


3889
Hypothetical protein ywbG [Bacillus
UniRef100_P39590
Bacillus subtilis
YwbG



subtilis]


3890
HlyC domain protein [Bacillus cereus]
UniRef100_Q731T5
Bacillus cereus


3891



OpuE


3892
Hypothetical UPF0064 protein ywbD
UniRef100_P39587
Bacillus subtilis
YwbD



[Bacillus subtilis]


3893
Hypothetical protein ywbC [Bacillus
UniRef100_P39586
Bacillus subtilis
YwbC



subtilis]


3894
Phosphoglycerate dehydrogenase
UniRef100_Q8GCC8
Bacillus
YvcT



[Bacillus coagulans]

coagulans










3895
Glycerate dehydrogenase
UniRef100_Q8EP33
Oceanobacillus iheyensis



[Oceanobacillus iheyensis]











3896
General stress protein A [Bacillus
UniRef100_P25148
Bacillus subtilis
GspA



subtilis]


3897
YdfH protein [Bacillus subtilis]
UniRef100_P96685
Bacillus subtilis
YdfH


3898
YdfI protein [Bacillus subtilis]
UniRef100_P96686
Bacillus subtilis
YdfI


3899
YdfJ protein [Bacillus subtilis]
UniRef100_P96687
Bacillus subtilis
YdfJ


3900
Hypothetical protein ywaF [Bacillus
UniRef100_P25149
Bacillus subtilis
YwaF



subtilis]


3901
MFS transporter, phthalate permease
UniRef100_Q884B5
Pseudomonas
YcbE



family [Pseudomonas syringae]

syringae


3902
Hypothetical protein OB2215
UniRef100_Q8EP98
Oceanobacillus
YitF



[Oceanobacillus iheyensis]

iheyensis


3903
2-dehydro-3-deoxyphosphogluconate
UniRef100_Q8EP99
Oceanobacillus
KdgA



aldolase [Oceanobacillus iheyensis]

iheyensis


3904
2-keto-3-deoxygluconate kinase
UniRef100_Q8EPA0
Oceanobacillus
KdgK



[Oceanobacillus iheyensis]

iheyensis


3905
Transcriptional regulator
UniRef100_Q8EPA1
Oceanobacillus
KipR



[Oceanobacillus iheyensis]

iheyensis


3906



YwaD


3907
Hypothetical protein ywoD [Bacillus
UniRef100_P94574
Bacillus subtilis
YwoD



subtilis]


3908


3909
Hypothetical protein ywaC [Bacillus
UniRef100_P39583
Bacillus subtilis
YwaC



subtilis]


3910


3911



DltA


3912
Protein dltB [Bacillus subtilis]
UniRef100_P39580
Bacillus subtilis
DltB


3913
D-alanine--poly(phosphoribitol) ligase
UniRef100_P39579
Bacillus subtilis



subunit 2 [Bacillus subtilis]


3914
Protein dltD precursor [Bacillus subtilis]
UniRef100_P39578
Bacillus subtilis
DltD


3915
Putative branched-chain-amino-acid
UniRef100_P39576
Bacillus subtilis
YwaA



aminotransferase [Bacillus subtilis]


3916
Putative macrolide-efflux determinant,
UniRef100_O32203
Bacillus subtilis
YvqJ



YvgJ [Bacillus subtilis]


3917
Probable 6-phospho-beta-glucosidase
UniRef100_P46320
Bacillus subtilis
LicH



[Bacillus subtilis]


3918
PTS system, lichenan-specific IIA
UniRef100_P46319
Bacillus subtilis
LicA



component [Bacillus subtilis]


3919
PTS system, lichenan-specific IIC
UniRef100_P46317
Bacillus subtilis
LicC



component [Bacillus subtilis]


3920
PTS system, lichenan-specific IIB
UniRef100_P46318
Bacillus subtilis



component [Bacillus subtilis]


3921
Probable licABCH operon regulator
UniRef100_P46321
Bacillus subtilis
LicR



[Bacillus subtilis]


3922


3923
Putative 3-methyladenine DNA
UniRef100_P94378
Bacillus subtilis
YxlJ



glycosylase [Bacillus subtilis]


3924
Transcriptional regulator [Rhizobium
UniRef100_Q988D9
Rhizobium loti
YdhC



loti]


3925
Citrate synthase III [Bacillus subtilis]
UniRef100_P45858
Bacillus subtilis
MmgD


3926
2-methylisocitrate dehydratase
UniRef100_Q6HJ23
Bacillus
MmgE



[Bacillus thuringiensis]

thuringiensis


3927
Putative carboxyvinyl-
UniRef100_P54528
Bacillus subtilis
YqiQ



carboxyphosphonate



phosphorylmutase [Bacillus subtilis]


3928
Chromate transporter [Bacillus
UniRef100_Q6HIU6
Bacillus
YwrA



thuringiensis]

thuringiensis










3929
BH0575 protein [Bacillus halodurans]
UniRef100_Q9KFB0
Bacillus halodurans











3930



YwjA


3931
YesT protein [Bacillus subtilis]
UniRef100_O31523
Bacillus subtilis
YesT


3932



YweA


3933



YweA


3934
Drug resistance transporter Bcr/CflA
UniRef100_Q6HPJ5
Bacillus
YdgK



subfamily; possible bicyclomycin

thuringiensis



resistance protein [Bacillus



thuringiensis]


3935
YxlG protein [Bacillus subtilis]
UniRef100_P94375
Bacillus subtilis
YxlG


3936
YxlF protein [Bacillus subtilis]
UniRef100_P94374
Bacillus subtilis
YxlF


3937
YxlE protein [Bacillus subtilis]
UniRef100_P94373
Bacillus subtilis


3938


3939
Hypothetical protein yxlC [Bacillus
UniRef100_P94371
Bacillus subtilis



subtilis]


3940
RNA polymerase sigma factor sigY
UniRef100_P94370
Bacillus subtilis
SigY



[Bacillus subtilis]


3941
YxlH protein [Bacillus subtilis]
UniRef100_P94376
Bacillus subtilis
YxlH


3942
Catalase [Bacillus halodurans]
UniRef100_Q9KDA8
Bacillus
KatX





halodurans


3943
Catalase [Bacillus subtilis]
UniRef100_Q9AQQ9
Bacillus subtilis
KatA


3944
Peroxide operon transcriptional
UniRef100_Q71YY4
Listeria
PerR



regulator [Listeria monocytogenes]

monocytogenes


3945
Ferrochelatase [Oceanobacillus
UniRef100_Q8ERX9
Oceanobacillus
HemH



iheyensis]

iheyensis


3946
Hypothetical protein yhcI [Bacillus
UniRef100_P54593
Bacillus subtilis
YhcI



subtilis]


3947
Bacitracin transport ATP-binding
UniRef100_Q81AB3
Bacillus cereus
YhcH



protein bcrA [Bacillus cereus]


3948
MrsE protein [Bacillus sp.]
UniRef100_Q9RC25
Bacillus sp.


3949


3950
ABC-2 type transport system ATP-
UniRef100_Q8ZZG9
Pyrobaculum
YdbJ



binding protein [Pyrobaculum

aerophilum



aerophilum]


3951










3952
Transcriptional regulator [Bacillus
UniRef100_Q9KFL9
Bacillus halodurans



halodurans]


3953











3954
ClyA protein [Plasmid pAD1]
UniRef100_Q52055
Plasmid pAD1
IspA


3955
Lantibiotic mersacidin transporter
UniRef100_Q9KFM8
Bacillus
YgaD



system [Bacillus halodurans]

halodurans


3956










3957
Lantibiotic mersacidin [Bacillus
UniRef100_Q9KFM5
Bacillus halodurans



halodurans]


3958
Lantibiotic mersacidin modifying
UniRef100_Q9KFM4
Bacillus halodurans



enzyme [Bacillus halodurans]











3959
Pectate lyase Pel-34K [Bacillus sp. P-
UniRef100_Q8L0S6
Bacillus sp. P-
Pel



2850]

2850


3960
Hypothetical UPF0031 protein yxkO
UniRef100_P94368
Bacillus subtilis
YxkO



[Bacillus subtilis]


3961
Transport ATP-binding protein cydD
UniRef100_P94367
Bacillus subtilis
CydD



[Bacillus subtilis]


3962
Transport ATP-binding protein cydC
UniRef100_P94366
Bacillus subtilis
CydC



[Bacillus subtilis]


3963
Cytochrome d ubiquinol oxidase
UniRef100_P94365
Bacillus subtilis
CydB



subunit II [Bacillus subtilis]


3964
Cytochrome d ubiquinol oxidase
UniRef100_P94364
Bacillus subtilis
CydA



subunit I [Bacillus subtilis]


3965
Hypothetical transport protein ywcJ
UniRef100_P39608
Bacillus subtilis
YwcJ



[Bacillus subtilis]


3966
Hypothetical protein [Bacillus cereus]
UniRef100_Q735J7
Bacillus cereus


3967
Probable NAD-dependent malic
UniRef100_P54572
Bacillus subtilis
MleA



enzyme 1 [Bacillus subtilis]


3968
Malate-2H(+)/Na(+)-lactate antiporter
UniRef100_P54571
Bacillus subtilis
MleN



[Bacillus subtilis]


3969



AnsB


3970
L-asparaginase [Bacillus subtilis]
UniRef100_P26900
Bacillus subtilis
AnsA


3971



AnsR


3972
Hypothetical protein ykgB [Bacillus
UniRef100_O34499
Bacillus subtilis
YkgB



subtilis]


3973
Hypothetical sensory transduction
UniRef100_P42421
Bacillus subtilis
YxdJ



protein yxdJ [Bacillus subtilis]


3974
Hypothetical sensor-like histidine
UniRef100_P42422
Bacillus subtilis
YxdK



kinase yxdK [Bacillus subtilis]


3975
Hypothetical ABC transporter ATP-
UniRef100_P42423
Bacillus subtilis
YxdL



binding protein yxdL [Bacillus subtilis]


3976
Hypothetical protein yxdM [Bacillus
UniRef100_P42424
Bacillus subtilis
YxdM



subtilis]


3977
Hypothetical protein yxeA precursor
UniRef100_P54940
Bacillus subtilis
YxeA



[Bacillus subtilis]


3978
YxkH protein [Bacillus subtilis]
UniRef100_P94361
Bacillus subtilis
YxkH


3979
Transcriptional regulator [Bacillus
UniRef100_Q9KFA7
Bacillus
YdeL



halodurans]

halodurans


3980
Hypothetical protein orfD [Lactococcus
UniRef100_O86288
Lactococcus
YdaM



lactis]

lactis


3981
Citrate/malate transporter [Bacillus
UniRef100_P94363
Bacillus subtilis
YxkJ



subtilis]


3982
Glycerophosphodiester
UniRef100_Q8EMK9
Oceanobacillus
YhdW



phosphodiesterase [Oceanobacillus

iheyensis



iheyensis]


3983
Hypothetical protein yhjA precursor
UniRef100_O07555
Bacillus subtilis



[Bacillus subtilis]


3984


3985
YfnI [Bacillus subtilis]
UniRef100_O06487
Bacillus subtilis
YfnI


3986
Hypothetical lipoprotein yfjD precursor
UniRef100_O31555
Bacillus subtilis
YfjD



[Bacillus subtilis]


3987
YxkD protein [Bacillus subtilis]
UniRef100_P94357
Bacillus subtilis
YxkD


3988
Arginine repressor, argR [Bacillus
UniRef100_Q81II2
Bacillus cereus
AhrC



cereus]


3989
Arginine deiminase [Bacillus cereus]
UniRef100_Q73E87
Bacillus cereus
YkgA


3990
Ornithine carbamoyltransferase
UniRef100_Q73E86
Bacillus cereus
ArgF



[Bacillus cereus]


3991
Arginine/ornithine antiporter [Bacillus
UniRef100_Q73E85
Bacillus cereus
YvsH



cereus]


3992
Carbamate kinase [Bacillus cereus]
UniRef100_Q81IH8
Bacillus cereus


3993
Transcriptional regulator, Crp family
UniRef100_Q6HP25
Bacillus
Fnr



[Bacillus thuringiensis]

thuringiensis


3994
Beta-glucosidase [Oceanobacillus
UniRef100_Q8ES64
Oceanobacillus
YdhP



iheyensis]

iheyensis


3995
PTS system, lichenan-specific IIc
UniRef100_Q6D101
Erwinia
LicC



component [Erwinia carotovora]

carotovora


3996
Hypothetical protein [Bacillus
UniRef100_Q6HC13
Bacillus thuringiensis



thuringiensis]


3997



SigV










3998
Anaerobic ribonucleoside-triphosphate
UniRef100_O26387
Methanobacterium



reductase activating protein

thermoautotrophicum



[Methanobacterium



thermoautotrophicum]


3999
Hypothetical protein yxeC [Bacillus
UniRef100_P54942
Bacillus subtilis



subtilis]











4000






4001


4002
Hypothetical protein [Anabaena sp.]
UniRef100_Q9AIM4
Anabaena sp.
FtsH


4003
Peptidase T [Bacillus subtilis]
UniRef100_P55179
Bacillus subtilis
PepT


4004
Beta-fructosidase FruA [Bacillus
UniRef100_Q8GM36
Bacillus
SacA



megaterium]

megaterium


4005
Sugar transporter FruP [Bacillus
UniRef100_Q8GM37
Bacillus
YwbF



megaterium]

megaterium


4006
ABC transporter membrane-spanning
UniRef100_Q8DNN9
Streptococcus
YesQ



permease-sugar transport

pneumoniae



[Streptococcus pneumoniae]


4007
ABC transporter membrane-spanning
UniRef100_Q8DNN8
Streptococcus
YurN



permease-sugar transporter

pneumoniae



[Streptococcus pneumoniae]


4008



YesO


4009
Repressor FruR [Bacillus megaterium]
UniRef100_Q8GM38
Bacillus
RbsR





megaterium


4010
Hypothetical protein [Bacillus cereus]
UniRef100_Q738I8
Bacillus cereus


4011



YjeA


4012
Hypothetical protein yxjG [Bacillus
UniRef100_P42318
Bacillus subtilis
YxjG



subtilis]


4013
YkcB protein [Bacillus subtilis]
UniRef100_O34575
Bacillus subtilis
YkcB


4014
Putative glycosyl transferase ykcC
UniRef100_O34319
Bacillus subtilis
YkcC



[Bacillus subtilis]


4015



GalE


4016


4017


4018










4019
Hypothetical 10.1 kDa protein [Bacillus
UniRef100_O50571
Bacillus pseudofirmus



pseudofirmus]


4020











4021
Hypothetical transport protein yxjA
UniRef100_P42312
Bacillus subtilis
YxjA



[Bacillus subtilis]


4022
Catalase 2 [Bacillus subtilis]
UniRef100_P42234
Bacillus subtilis
KatE


4023
Catalase 2 [Bacillus subtilis]
UniRef100_P42234
Bacillus subtilis
KatE


4024
DNA gyrase inhibitory protein, GyrI
UniRef100_Q97DI7
Clostridium
YosT



[Clostridium acetobutylicum]

acetobutylicum


4025
Probable glycosyl hydrolase [Erwinia
UniRef100_Q6D774
Erwinia
YckE



carotovora]

carotovora


4026
PTS system, beta-glucoside-specific
UniRef100_Q9KG19
Bacillus
BglP



enzyme II, ABC component [Bacillus

halodurans



halodurans]


4027
Transcription antiterminator licT
UniRef100_P39805
Bacillus subtilis
LicT



[Bacillus subtilis]


4028
Hypothetical conserved protein
UniRef100_Q8ES40
Oceanobacillus
YkoC



[Oceanobacillus iheyensis]

iheyensis


4029
Cation ABC transporter ATP-binding
UniRef100_Q8ES39
Oceanobacillus
YkoD



protein [Oceanobacillus iheyensis]

iheyensis


4030
Hypothetical conserved protein
UniRef100_Q8ES38
Oceanobacillus
YkoE



[Oceanobacillus iheyensis]

iheyensis


4031
Transcriptional activator of extracellular
UniRef100_Q8ESZ0
Oceanobacillus
TenA



enzyme genes [Oceanobacillus

iheyensis



iheyensis]


4032
Isocitrate lyase [Bacillus cereus]
UniRef100_Q73C38
Bacillus cereus
YqiQ


4033
Malate synthase A [Bacillus cereus]
UniRef100_Q73C39
Bacillus cereus


4034


4035
Hypothetical protein yycA [Bacillus
UniRef100_P37483
Bacillus subtilis
YycA



subtilis]


4036
Bacitracin export permease protein
UniRef100_O34741
Bacillus subtilis
YtsD



bceB [Bacillus subtilis]


4037
Bacitracin export ATP-binding protein
UniRef100_O34697
Bacillus subtilis
YtsC



bceA [Bacillus subtilis]


4038
Sensor protein bceS [Bacillus subtilis]
UniRef100_O35044
Bacillus subtilis
YtsB


4039
Sensory transduction protein bceR
UniRef100_O34951
Bacillus subtilis
YtsA



[Bacillus subtilis]


4040
Beta-glucosidase [Bacillus subtilis]
UniRef100_P40740
Bacillus subtilis
BglH


4041
PTS system, beta-glucoside-specific
UniRef100_P40739
Bacillus subtilis
BglP



IIABC component [Bacillus subtilis]


4042
Hypothetical protein yxeG [Bacillus
UniRef100_P54946
Bacillus subtilis
YxeG



subtilis]


4043
Hypothetical protein yxeI [Bacillus
UniRef100_P54948
Bacillus subtilis
YxeI



subtilis]


4044


4045


4046
Hypothetical protein yxiA precursor
UniRef100_P42293
Bacillus subtilis
YxiA



[Bacillus subtilis]


4047
ATP-dependent RNA helicase dbpA
UniRef100_P42305
Bacillus subtilis
DeaD



[Bacillus subtilis]










4048
Hypothetical protein OB2811
UniRef100_Q8EMN1
Oceanobacillus iheyensis



[Oceanobacillus iheyensis]


4049
Hypothetical protein OB2810
UniRef100_Q8EMN2
Oceanobacillus iheyensis



[Oceanobacillus iheyensis]











4050



YxiD


4051


4052
BH4015 protein [Bacillus halodurans]
UniRef100_Q9K5S3
Bacillus
YxiB





halodurans


4053
Pyrimidine-nucleoside phosphorylase
UniRef100_P39142
Bacillus subtilis
Pdp



[Bacillus subtilis]


4054
Pyrimidine nucleoside transport protein
UniRef100_P39141
Bacillus subtilis
NupC



[Bacillus subtilis]


4055
Deoxyribose-phosphate aldolase
UniRef100_P39121
Bacillus subtilis
Dra



[Bacillus subtilis]


4056
Deoxyribonucleoside regulator [Bacillus
UniRef100_P39140
Bacillus subtilis
DeoR



subtilis]


4057
Hypothetical protein [Bacillus anthracis]
UniRef100_Q81X49
Bacillus anthracis


4058
YdaJ protein [Bacillus subtilis]
UniRef100_P96584
Bacillus subtilis
YdaJ


4059
YdaK protein [Bacillus subtilis]
UniRef100_P96585
Bacillus subtilis
YdaK


4060
YdaL protein [Bacillus subtilis]
UniRef100_O31487
Bacillus subtilis
YdaL


4061
YdaM protein [Bacillus subtilis]
UniRef100_P96587
Bacillus subtilis
YdaM


4062
YdaN protein [Bacillus subtilis]
UniRef100_O31488
Bacillus subtilis
YdaN


4063
Valine-pyruvate aminotransferase
UniRef100_Q8YTB2
Anabaena sp.
AlaT



[Anabaena sp.]


4064
Hypothetical protein [Leifsonia xyli]
UniRef100_Q6AEW0
Leifsonia xyli


4065
Hypothetical protein [Leifsonia xyli]
UniRef100_Q6AEW1
Leifsonia xyli
YwfB


4066
Similarities with putative carboxylase
UniRef100_Q7NA29
Photorhabdus
YwfE



[Photorhabdus luminescens]

luminescens


4067
Transporter, Drug/Metabolite Exporter
UniRef100_Q63AK7
Bacillus cereus
YoaV



family [Bacillus cereus ZK]

ZK


4068
Probable fructose-bisphosphate
UniRef100_P42420
Bacillus subtilis
FbaB



aldolase 2 [Bacillus subtilis]


4069
IolI protein [Bacillus subtilis]
UniRef100_P42419
Bacillus subtilis
IolI


4070
IolH protein [Bacillus subtilis]
UniRef100_P42418
Bacillus subtilis
IolH


4071
Myo-inositol dehydrogenase [Bacillus
UniRef100_Q6B6R7
Bacillus subtilis
Idh



subtilis]


4072
Myo-inositol transport protein [Bacillus
UniRef100_P42417
Bacillus subtilis
IolF



subtilis]


4073
IolE protein [Bacillus subtilis]
UniRef100_P42416
Bacillus subtilis
IolE


4074
Probable malonic semialdehyde
UniRef100_P42415
Bacillus subtilis
IolD



oxidative decarboxylase [Bacillus



subtilis]


4075
Protein ioIC [Bacillus subtilis]
UniRef100_P42414
Bacillus subtilis
IolC


4076
IolB protein [Bacillus subtilis]
UniRef100_P42413
Bacillus subtilis
IolB


4077
Probable methylmalonate-
UniRef100_P42412
acylating
MmsA



semialdehyde dehydrogenase



[acylating] [Bacillus subtilis]


4078
DNA-binding protein iolR [Bacillus
UniRef100_P46337
Bacillus subtilis
IolR



subtilis]


4079
IolS protein [Bacillus subtilis]
UniRef100_P46336
Bacillus subtilis
IolS










4080
UPI00003CB380 UniRef100 entry
UniRef100_UPI00003CB380
GlpQ











4081
Putative glycerol-3-phosphate
UniRef100_Q6GJY0
Staphylococcus
GlpT



transporter [Staphylococcus aureus]

aureus


4082
Chaperone protein htpG [Bacillus
UniRef100_P46208
Bacillus subtilis
HtpG



subtilis]


4083



YfmM


4084
Hypothetical protein yxeH [Bacillus
UniRef100_P54947
Bacillus subtilis
YxeH



subtilis]


4085
Glycerol dehydrogenase [Clostridium
UniRef100_Q97IL4
Clostridium
AraM



acetobutylicum]

acetobutylicum


4086
Phosphoserine phosphatase family
UniRef100_Q97IL5
Clostridium
YkrX



protein [Clostridium acetobutylicum]

acetobutylicum










4087
BH0833 protein [Bacillus halodurans]
UniRef100_Q9KEL9
Bacillus halodurans











4088



YxeB


4089


4090
BH3956 protein [Bacillus halodurans]
UniRef100_Q9K5Y0
Bacillus
YraH





halodurans


4091










4092
Ferrous iron transport protein B
UniRef100_Q632N2
Bacillus cereus ZK



[Bacillus cereus ZK]


4093
Lmo2104 protein [Listeria
UniRef100_Q929R6
Listeria monocytogenes



monocytogenes]











4094
YfiQ protein [Bacillus subtilis]
UniRef100_O31559
Bacillus subtilis
YfiQ


4095
ABC transporter permease protein
UniRef100_Q81EI1
Bacillus cereus
YxdM



[Bacillus cereus]


4096
ABC transporter ATP-binding protein
UniRef100_Q81D39
Bacillus cereus
YxdL



[Bacillus cereus]


4097
Sensor histidine kinase [Bacillus
UniRef100_Q63AY4
Bacillus cereus
YxdK



cereus ZK]

ZK










4098
UPI00003CBD9F UniRef100 entry
UniRef100_UPI00003CBD9F
YxdJ











4099
Hypothetical protein [Bacillus cereus]
UniRef100_Q72X82
Bacillus cereus



4100
YoaH [Bacillus subtilis]
UniRef100_O34576
Bacillus subtilis
YoaH


4101
Amino acid transporter [Bacillus
UniRef100_Q9K609
Bacillus
YflA



halodurans]

halodurans


4102
Alanine dehydrogenase [Bacillus
UniRef100_Q9KAF8
Bacillus
Ald



halodurans]

halodurans


4103
Hypothetical family 53 glycosyl
UniRef100_O07013
Bacillus subtilis
YvfO



hydrolase yvfO precursor [Bacillus



subtilis]


4104
Hypotheticai protein yvfN [Baciiius
UniRef1000_O07012
Baciiius subtilis
LacA



subtilis]


4105
Hypothetical protein yvfM [Bacillus
UniRef100_O07011
Bacillus subtilis
YvfM



subtilis]


4106
Maltose/maltodextrin transport system
UniRef100_Q9KBA8
Bacillus
YvfL



[Bacillus halodurans]

halodurans


4107
Hypothetical protein yvfK [Bacillus
UniRef100_O07009
Bacillus subtilis
YvfK



subtilis]


4108



LacR


4109
Galactokinase [Streptococcus gordonii]
UniRef100_Q840N8
Streptococcus
Galk





gordonii


4110
UDP-glucose 4-epimerase [Listeria
UniRef100_Q928B6
Listeria innocua
GalE



innocua]










4111
UPI00003CB9B6 UniRef100 entry
UniRef100_UPI00003CB9B6
GalT











4112
Transcriptional repressor of the xylose
UniRef100_Q9KDW7
Bacillus
XylR



operon [Bacillus halodurans]

halodurans


4113



GntR


4114



GntK


4115



GntP


4116



GntZ


4117
Alcohol dehydrogenase [Bacillus
UniRef100_Q818A4
Bacillus cereus
GbsB



cereus]


4118
Alkyl hydroperoxide reductase subunit
UniRef100_P80239
Bacillus subtilis
AhpC



C [Bacillus subtilis]


4119
NADH dehydrogenase [Bacillus
UniRef100_P42974
Bacillus subtilis
AhpF



subtilis]


4120


4121


4122


4123



YvfR


4124
Hypothetical protein yvfS [Bacillus
UniRef100_O07017
Bacillus subtilis
YvfS



subtilis]


4125



YvfT


4126
Hypothetical protein yvfU [Bacillus
UniRef100_O07019
Bacillus subtilis
YvfU



subtilis]


4127


4128


4129



YtrE


4130


4131


4132


4133










4134
Hypothetical protein [Desulfotalea
UniRef100_Q6ARQ4
Desulfotalea psychrophila



psychrophila]


4135
Hypothetical protein [Desulfotalea
UniRef100_Q6ARQ5
Desulfotalea psychrophila



psychrophila]


4136


4137











4138
Putative zinc metallopeptidase
UniRef100_Q894N2
Clostridium




[Clostridium tetani]

tetani










4139
Type I restriction-modification system
UniRef100_Q6MH63
Bdellovibrio bacteriovorus



restriction subunit [Bdellovibrio



bacteriovorus]











4140
Lin0523 protein [Listeria innocua]
UniRef100_Q92ED7
Listeria innocua



4141










4142
Hypothetical protein CAC1662
UniRef100_Q97IH9
Clostridium acetobutylicum



[Clostridium acetobutylicum]


4143
Type I restriction-modification system,
UniRef100_Q72BC7
Desulfovibrio vulgaris



M subunit [Desulfovibrio vulgaris]


4144


4145


4146










4147
UPI00002C7192 UniRef100 entry
UniRef100_UPI00002C7192












4148
Cassette chromosome recombinase B
UniRef100_Q8RPD2
Staphylococcus
SpoIVCA



[Staphylococcus aureus]

aureus


4149
Hypothetical UPF0247 protein yyda
UniRef100_Q45601
Bacillus subtilis
YydA



[Bacillus subtilis]


4150


4151


4152
YycN protein [Bacillus subtilis]
UniRef100_O32293
Bacillus subtilis
YycN


4153










4154
UPI00003CA59D UniRef100 entry
UniRef100_UPI00003CA59D












4155
Hypothetical serine protease yyxA
UniRef100_P39668
Bacillus subtilis
YyxA



[Bacillus subtilis]


4156
YycJ protein [Bacillus subtilis]
UniRef100_Q45611
Bacillus subtilis
YycJ


4157
YycI protein [Bacillus subtilis]
UniRef100_Q45612
Bacillus subtilis
YycI


4158
YycH protein [Bacillus subtilis]
UniRef100_Q45613
Bacillus subtilis
YycH


4159
Sensor protein yycG [Bacillus subtilis]
UniRef100_Q45614
Bacillus subtilis
YycG


4160
Transcriptional regulatory protein yycF
UniRef100_P37478
Bacillus subtilis
YycF



[Bacillus subtilis]


4161
Phosphohydrolase [Bacillus cereus]
UniRef100_Q81I03
Bacillus cereus


4162
Adenylosuccinate synthetase [Bacillus
UniRef100_P29726
Bacillus subtilis
PurA



subtilis]


4163


4164
Replicative DNA helicase [Bacillus
UniRef100_P37469
Bacillus subtilis
DnaC



subtilis]


4165
Hypothetical protein yycD [Bacillus
UniRef100_P37480
Bacillus subtilis



subtilis]


4166
Hypothetical protein yyzB [Bacillus
UniRef100_O32296
Bacillus subtilis



subtilis]


4167
Hypothetical transport protein yycB
UniRef100_P37482
Bacillus subtilis
YycB



[Bacillus subtilis]


4168
N-acetylglucosamine-6-phosphate
UniRef100_Q9KFQ7
Bacillus
NagA



deacetylase [Bacillus halodurans]

halodurans


4169



NagB


4170
Transcriptional regulator [Bacillus
UniRef100_Q9KFQ9
Bacillus
YvoA



halodurans]

halodurans


4171
Hypothetical protein VP0543 [Vibrio
UniRef100_Q87S81
Vibrio
YbbI



parahaemolyticus]

parahaemolyticus


4172
PTS system, n-acetylglucosamine-
UniRef100_Q9KF24
Bacillus
NagP



specific enzyme II, ABC component

halodurans



[Bacillus halodurans]


4173
50S ribosomal protein L9 [Bacillus
UniRef100_P37437
Bacillus subtilis
RplI



subtilis]


4174
Hypothetical protein yybT [Bacillus
UniRef100_P37484
Bacillus subtilis
YybT



subtilis]


4175
Hypothetical protein yybS [Bacillus
UniRef100_P37485
Bacillus subtilis
YybS



subtilis]


4176
Spore coat protein F precursor
UniRef100_P23261
Bacillus subtilis
CotF



[Bacillus subtilis]


4177
Universal stress protein, Usp family
UniRef100_Q6HIV0
Bacillus
YxiE



[Bacillus thuringiensis]

thuringiensis


4178
Sulfate permease [Bacillus
UniRef100_Q6HIU9
Bacillus
YbaR



thuringiensis]

thuringiensis


4179
Hypothetical transport protein yybO
UniRef100_P37489
Bacillus subtilis
YybO



[Bacillus subtilis]


4180
30S ribosomal protein S18 [Bacillus
UniRef100_P21475
Bacillus subtilis



subtilis]


4181
Single-strand binding protein [Bacillus
UniRef100_P37455
Bacillus subtilis
Ssb



subtilis]


4182
30S ribosomal protein S6 [Bacillus
UniRef100_P21468
Bacillus subtilis



subtilis]


4183
GTP-dependent nucleic acid-binding
UniRef100_P37518
Bacillus subtilis
YyaF



protein engD [Bacillus subtilis]


4184
Lin2921 protein [Listeria innocua]
UniRef100_Q926W9
Listeria innocua


4185
Hypothetical protein yyaD [Bacillus
UniRef100_P37520
Bacillus subtilis
YyaD



subtilis]


4186
Hypothetical protein yyaC [Bacillus
UniRef100_P37521
Bacillus subtilis
YyaC



subtilis]


4187
Stage 0 sporulation protein J [Bacillus
UniRef100_P26497
Bacillus subtilis
Spo0J



subtilis]


4188



Soj


4189
Hypothetical protein [Burkholderia
UniRef100_Q63L10
Burkholderia
YdfG



pseudomallei K96243]

pseudomallei





K96243


4190
RNA polymerase sigma factor, ECF
UniRef100_Q63BC0
Bacillus cereus
SigM



subfamily [Bacillus cereus ZK]

ZK


4191
YyaA protein [Bacillus subtilis]
UniRef100_P37524
Bacillus subtilis
YyaA


4192
Methyltransferase gidB [Bacillus
UniRef100_P25813
Bacillus subtilis
GidB



subtilis]


4193
Glucose inhibited division protein A
UniRef100_P25812
Bacillus subtilis
GidA



[Bacillus subtilis]


4194
Probable tRNA modification GTPase
UniRef100_P25811
Bacillus subtilis
ThdF



trmE [Bacillus subtilis]


4195
Jag protein [Bacillus subtilis]
UniRef100_Q01620
Bacillus subtilis
Jag


4196



SpolllJ


4197
Ribonuclease P protein component
UniRef100_P25814
Bacillus subtilis
RnpA



[Bacillus subtilis]
















TABLE 2







Features of the Bacillus licheniformis genome and comparison with genomes of other Bacillus species.













Feature
B. licheniformis
B. subtilisa
B. haloduransb
Oceanobacillus iheyensisc
B. anthracisd
B. cereuse
















Chromosome Size (bp)
4,222,336
4,214,630
4,202,352
3,630,528
5,227,293
5,426,909


G + C content (mol %)
46.2
43.5
43.7
35.7
35.4
35.4


Protein coding sequences
4197
4106
4066
3496
5508
5366


Average length (bp)
873
896
879
883
800
835


Percent of coding region
87
87
85
85
84
84


Ribosomal RNA operons
7
10
8
7
11
13


Number of tRNAs
81
86
78
69
95
108


Phage-associated genes
71
268
42
27
62
124


Transposase genes of
10
0
93
14
18
10


IS-elements





aKunst, F., Ogasawara, N, Mozser, I., Albertini, A. M., Alloni, G., Azebedo, V., Bertero, M. G., Bessieres, P., Bolotin, A., and Borchert, S. et al. (1997) Nature 390, 249-256.


bTakami, H., Nakasone, K., Takaki, Y., Maeno, G., Sasaki, R., Masui, N., Fuji, F., Hirama, C., Nakamura, Y., Ogasawara, N. et al. (2000) Nucleic Acids Res. 28, 4317-4331.


cTakami, H., Takaki, Y., and Uchiyama, I. (2002) Nucleic Acids Res. 30, 3927-3935.


dRead, T. D., Peterson, S. N., Tourasse, N., Baillie, L. W., Paulsen, I. T., Nelson, K. E., Tettelin, H., Fouts, D. E., Eisen, J. A., and Gill, S. R. et al. (2003) Nature 423, 81-86.


eIvanova, N, Sorokin, A., Anderson, I, Galleron, N., Candelon, B., Kapatral, V., Bhattacharyya, A., Reznik, G., Mikhailova, N., and Lapidus, A. et al. (2003) Nature 423, 87-91.













TABLE 3







Extracellular proteins predicted in the Bacillus licheniformis genome














Signal





SEQ ID
Gene
Peptide


B. subtilis


NO.
Name
Location
Putative Product
Putative Function
Gene















11
dacA
[1-32]
D-alanyl-D-alanine
“Molecular Function: serine
DacA





carboxypeptidase (penicillin-
carboxypeptidase activity





binding protein 5)
(GO: 0004185), Biological






Process: proteolysis and






peptidolysis (GO: 0006508)”











45
yabE
[1-32]
conserved hypothetical containing domain DUF348 YabE
YabE












67
divlC
[1-66]
cell-division initiation protein
“required for both vegetative and
DivlC






sporulation septum formation,






Biological Process: cell cycle






(GO: 0007049)”


157
BL01016
[1-28]
N-acetylmuramoyl-L-alanine
“cell wall hydrolase, Molecular
CwlD





amidase
Function: N-acetylmuramoyl-L-






alanine amidase activity






(GO: 0008745), Biological






Process: peptidoglycan






catabolism (GO: 0009253)”


159
gerD
[1-27]
GerD
“germination response to L-
GerD






alanine and to the combination






of glucose, fructose, L-






asparagine, and KCl (early






stage),”


161
ybaN
[1-42]
“Polysaccharide
“Biological Process:
YbaN





deacetylase, Carbohydrate
carbohydrate metabolism





Esterase Family 4”
(GO: 0005975), Molecular






Function: hydrolase activity,






acting on carbon-nitrogen (but






not peptide) bonds






(GO: 0016810)”


162
pbpX
[1-43]
penicillin-binding protein

PbpX











167
ybbC
[1-23]
conserved hypothetical protein YbbC
YbbC












168
ybbD
[1-27]
“Glycoside hydrolase, family 3”
“Molecular Function: hydrolase
YbbD






activity, hydrolyzing O-glycosyl






compounds (GO: 0004553),






Biological Process:






carbohydrate metabolism






(GO: 0005975)”


169
ybbE
[1-22]
putative beta-lactamase YbbE

YbbE


227

[1-34]
putative ribose ABC transporter
“ribose transport,”
RbsB





(ribose-binding protein)











228
yomI
[1-32]
putative lytic transglycosylase YomI
YomI












247
penP
[1-35]
beta-lactamase precursor
“Molecular Function: aspartic-
PenP






type endopeptidase activity






(GO: 0004190), Biological






Process: proteolysis and






peptidolysis (GO: 0006508)”











249
tatAD
[1-35]
component of the twin-arginine
“Biological Process: protein transport





pre-protein translocation
(GO: 0015031), Cellular Component: integral





pathway
to membrane (GO: 0016021)”


267

[1-26]
“putative Proteinase inhibititor
Molecular Function: serine-type





I4, serpin”
endopeptidase inhibitor activity






(GO: 0004867)












268
BL01663
[1-22]
Pectin lyase-like

YbdN


287
BL01793
[1-31]
putative lipoprotein

YcdA


303
yvbX
[1-33]
“conserved protein, Glycoside
“Molecular Function: catalytic
YvbX





Hydrolase Family 18, YvbX”
activity (GO: 0003824),






Biological Process:






carbohydrate metabolism






(GO: 0005975)”











304

[1-27]
“Chitinase precursor, Glycoside
“Molecular Function: catalytic activity





Hydrolase Family 18”
(GO: 0003824), Biological Process:






carbohydrate metabolism (GO: 0005975)”











312
yvcE
[1-31]
“putative peptidoglycan hydrolase, DL-endopeptidase II family”
YvcE












348
yckB
[1-34]
putative extracellular solute-
“Molecular Function: transporter
YckB





binding protein YckB
activity (GO: 0005215),






Biological Process: transport






(GO: 0006810), Cellular






Component: periplasmic space






(sensu Gram-negative Bacteria)






(GO: 0030288)”


353
nucA
[1-37]
nuclease NucA

NucA


359
BL01722
[1-34]
putative extracellular solute-
“Molecular Function: transporter
YvfK





binding protein
activity (GO: 0005215),






Biological Process: transport






(GO: 0006810)”


373

[1-25]
hypothetical protein


382
BL01829
[1-25]
“putative extracellular solute-
“Molecular Function: transporter
YckK





binding protein, family 3”
activity (GO: 0005215),






Biological Process: transport






(GO: 0006810), Cellular






Component: periplasmic space






(sensu Gram-negative Bacteria)






(GO: 0030288)”


403
BL01746
[1-28]
ribose ABC transporter (ribose-
“ribose transport,”
RbsB





binding protein)


409
phy
[1-30]
phytase
“hydrolysis of phytate into
Phy






inorganic phosphate and myo-






inositol,”


426
yclQ
[1-25]
Periplasmic binding protein
“Molecular Function: iron ion
YclQ






transporter activity






(GO: 0005381), Biological






Process: high affinity iron ion






transport (GO: 0006827)”


438

[1-26]
hypothetical protein











472
BL02821
[1-34]
Cell envelope-related transcriptional attenuator
YvhJ










483

[1-42]
hypothetical protein











513
ydcC
[1-28]
conserved membrane protein YdcC
YdcC











552
yrhM
[1-68]
YrhM
YrhM











558
ywpE
[1-26]
conserved protein YwpE
“Biological Process: biosynthesis






(GO: 0009058), Molecular Function:






transferase activity (GO: 0016740)”


576

[1-34]
putative transporter
“Molecular Function: transporter activity,






(GO: 0005215), Biological Process: transport






(GO: 0006810), Cellular Component:






membrane (GO: 0016020)”












605
yjeAA
[1-37]
conserved hypothetical protein

YjeA


607
amyL
[1-30]
“alpha amylase, Glycoside
“Molecular Function: alpha-
TreA





Hydrolase Family 13”
amylase activity (GO: 0004556),






Biological Process:






carbohydrate metabolism






(GO: 0005975)”


610
yvdG
[1-28]
putative maltose/maltodextrin
“Molecular Function: transporter
YvdG





transport system substrate-
activity (GO: 0005215),





binding protein
Biological Process: transport






(GO: 0006810)”


619

[1-37]
hypothetical protein


620

[1-28]
hypothetical protein


621
ydjM
[1-41]
YdjM

YdjM


622
ydjN
[1-21]
YdjN

YdjN


660
yerB
[1-28]
conserved protein YerB

YerB


665
yerH
[1-26]
YerH

YerH











683
BL05063
[1-35]
mannan endo-1,4-beta-
Degradation of mannan polysaccharides





mannosidase


733

[1-29]
putative carboxypeptidase
“Molecular Function: carboxypeptidase A






activity (GO: 0004182), Biological Process:






proteolysis and peptidolysis (GO: 0006508)”












773
yfkD
[1-26]
conserved protein YfkD

YfkD


776
BL03088
[1-24]
“Polysaccharide deacetylase,
“Biological Process:
YfjS





Carbohydrate Esterase Family
carbohydrate metabolism





4”
(GO: 0005975), Molecular






Function: hydrolase activity,






acting on carbon-nitrogen (but






not peptide) bonds






(GO: 0016810)”


784
yfjL
[1-33]
putative transporter YfjL
“Molecular Function: transporter
YfjL






activity (GO: 0005215),






Biological Process: transport






(GO: 0006810), Cellular






Component: membrane






(GO: 0016020)”


787
BL03096
[1-26]
hypothetical protein


813
BL03024
[1-26]
“putative extracellular solute-
“Molecular Function: transporter
YtcQ





binding protein, family 1”
activity (GO: 0005215),






Biological Process: transport






(GO: 0006810)”


841
appAC
[1-30]
oligopeptide ABC transporter
“required for initiation of
OppA





(binding protein)
sporulation, competence






development, and oligopeptide






transport, Molecular Function:






transporter activity






(GO: 0005215), Biological






Process: transport






(GO: 0006810)”











866
ssuA
[1-24]
aliphatic sulfonate ABC transporter (binding lipoprotein)
SsuA












876

[1-23]
hypothetical protein




886

[1-23]
hypothetical protein


892

[1-28]
hypothetical protein

YhcC


900
BL03178
[1-27]
putative lipoprotein

YhcJ


902
BL03161
[1-31]
hypothetical protein

YhcM


904
BL03176
[1-29]
hypothetical protein

YhcN


905
BL03175
[1-30]
hypothetical protein

YhcP


934
IytE
[1-26]
cell wall hydrolase phosphatase-
“cell wall lytic activity, Biological
LytE





associated protein
Process: cell wall catabolism






(GO: 0016998), Biological






Process: cell wall catabolism






(GO: 0016998)”











937

[1-26]
putative glucose dehydrogenase













965
BL02841
[1-41]
“Polysaccharide deacetylase,
“Biological Process:
YheN





Carbohydrate Esterase Family
carbohydrate metabolism





4”
(GO: 0005975), Molecular






Function: hydrolase activity,






acting on carbon-nitrogen (but






not peptide) bonds






(GO: 0016810)”


996
prsA
[1-30]
molecular chaperone PrsA
“essential for the stability of
PrsA






secreted proteins at stages






following translocation across






the membrane, Molecular






Function: isomerase activity






(GO: 0016853)”


1001
BL02907
[1-31]
hypothetical protein

YhaH


1032
yhfQ
[1-32]
putative transferase
“Biological Process: metabolism
YhfQ






(GO: 0008152), Molecular






Function: transferase activity






(GO: 0016740)”


1044
epr
[1-27]
extracellular serine protease
“Molecular Function: subtilase
Epr






activity (GO: 0004289),






Biological Process: proteolysis






and peptidolysis






(GO: 0006508), Molecular






Function: subtilase activity






(GO: 0004289), Biological






Process: proteolysis and






peptidolysis (GO: 0006508)”


1061

[1-24]
hypothetical protein


1063
msmE
[1-29]
mutiple sugar-binding protein
“Molecular Function: transporter
MsmE





MsmE
activity (GO: 0005215),






Biological Process: transport






(GO: 0006810)”


1073
BL01323
[1-28]
conserved hypothetical protein

LytB


1077
yvgL
[1-29]
molybdate transport system
“Molecular Function: molybdate-
YvgL





substrate-binding protein
transporting ATPase activity






(GO: 0015412), Biological






Process: molybdate ion






transport (GO: 0015689)”


1113
BL01309
[1-24]
Short-chain
“Biological Process: metabolism
YcdF





dehydrogenase/reductase SDR
(GO: 0008152), Molecular






Function: oxidoreductase






activity (GO: 0016491)”


1150
appA
[1-32]
oligopeptide ABC transporter
“oligopeptide
AppA





(oligopeptide-binding protein)
transport, Molecular Function:






transporter activity






(GO: 0005215), Biological






Process: transport






(GO: 0006810)”


1156
oppA
[1-29]
oligopeptide ABC transporter
“required for initiation of
OppA





(binding protein)
sporulation, competence






development, and oligopeptide






transport, Molecular Function:






transporter activity






(GO: 0005215), Biological






Process: transport






(GO: 0006810)”


1165
BL03332
[1-31]
conserved hypothetical protein

YflP


1216
abnAA
[1-26]
Glycoside Hydrolase Family 43
“degradation of plant cell wall
AbnA






polysaccharide, Molecular






Function: hydrolase activity,






hydrolyzing O-glycosyl






compounds (GO: 0004553),






Biological Process:






carbohydrate metabolism






(GO: 0005975)”


1219

[1-30]
hypothetical protein


1226
BL01957
[1-23]
conserved hypothetical protein

YoeB


1227
BL01953
[1-26]
Peptidoglycan-binding protein
“Biological Process: cell wall
YocH






catabolism






(GO: 0016998), Biological






Process: cell wall catabolism






(GO: 0016998)”


1283
yesO
[1-28]
putative transport system
“Molecular Function: transporter
YesO





substrate-binding protein YesO
activity (GO: 0005215),






Biological Process: transport






(GO: 0006810)”











1291
yesW
[1-33]
putative polysaccharide lyase family 11 protein
YesW












1299
lplA
[1-32]
lipoprotein
“Molecular Function: transporter
LplA






activity (GO: 0005215),






Biological Process: transport






(GO: 0006810)”


1311
dppE
[1-27]
dipeptide ABC transporter
“Molecular Function: transporter
DppE





(dipeptide-binding protein)
activity (GO: 0005215),






Biological Process: transport






(GO: 0006810)”


1319
pelI
[1-25]
“pectate lyase family 1, PelI”

Pel


1332

[1-30]
hypothetical protein


1341

[1-27]
hypothetical protein


1345

[1-27]
hypothetical protein

YonS


1368

[1-28]
hypothetical protein


1415
pbpC
[1-30]
penicillin-binding protein 3
Molecular Function: penicillin
PbpC






binding (GO: 0008658)


1448

[1-28]
hypothetical protein


1475
BL05139
[1-27]
glycerophosphoryl diester
“hydrolysis of deacylated
GIpQ





phosphodiesterase
phospholipids, Biological






Process: glycerol metabolism






(GO: 0006071), Molecular






Function: glycerophosphodiester






phosphodiesterase activity






(GO: 0008889)”


1479

[1-27]
hypothetical protein


1493

[1-27]
hypothetical protein


1496
BL03556
[1-25]
putative Cell wall hydrolase

YkvT


1498
ykvV
[1-28]
hypothetical protein
Contains thioredoxin domain 2
YkvV


1501

[1-25]
hypothetical protein


1512
ykwC
[1-24]
Hypothetical oxidoreductase

YkwC


1513
BL03646
[1-27]
Allergen V5/Tpx-1 related
Cellular Component:
YkwD






extracellular (GO: 0005576)


1527

[1-28]
hypothetical protein


1553
BL01601
[1-32]
“putative secretion protein,
“Molecular Function: protein
YknX





protein transporter”
transporter activity






(GO: 0008565), Biological






Process: protein secretion






(GO: 0009306), Cellular






Component: membrane






(GO: 0016020)”


1565

[1-40]
hypothetical protein


1576
ykyA
[1-29]
Hypothetical protein ykyA

YkyA


1601
BL02997
[1-27]
hypothetical protein

YlaJ


1617
ylbC
[1-34]
conserved hypothetical protein
Cellular Component:
YlbC





YlbC
extracellular (GO: 0005576)


1625
ylbL
[1-29]
YlbL
Molecular Function: protein
YlbL






binding (GO: 0005515)


1635
ftsL
[1-59]
cell-division protein
“septum formation (early
FtsL






stage),”


1636
pbpB
[1-42]
penicillin-binding protein 2B
“formation of the cell-division
PbpB






septum (late stage),”


1644
divIB
[1-57]
cell-division initiation protein
“probably involved in stabilizing
DivIB






or promoting the assembly of






the division complex (septum






formation),”


1645
BL02246
[1-29]
conserved hypothetical protein

YlxW


1646
BL02248
[1-30]
hypothetical protein

YlxX


1650
bprA
[1-28]
bacillopeptidase F
“Molecular Function: subtilase
Bpr






activity (GO: 0004289),






Biological Process: proteolysis






and peptidolysis (GO: 0006508)”


1651
bprB
[1-31]
bacillopeptidase F
“Molecular Function: subtilase
Bpr






activity (GO: 0004289),






Biological Process: proteolysis






and peptidolysis (GO: 0006508)”


1752
fliL
[1-32]
flagellar protein
“required for flagellar
FliL






formation, Biological Process:






ciliary/flagellar motility






(GO: 0001539), Biological






Process: chemotaxis






(GO: 0006935), Cellular






Component: flagellar basal body






(sensu Bacteria) (GO: 0009425)”











1781
celA
[1-34]
Glycoside Hydrolase Family 9
“Molecular Function: hydrolase activity,






hydrolyzing O-glycosyl compounds






(GO: 0004553), Biological Process:






carbohydrate metabolism (GO: 0005975)”


1783
celC
[1-34]
Glycoside Hydrolase Family 5
“Molecular Function: hydrolase activity,






hydrolyzing O-glycosyl compounds






(GO: 0004553), Biological Process:






carbohydrate metabolism (GO: 0005975)”


1784
celD
[1-32]
Glycoside Hydrolase family 5
“Molecular Function: hydrolase activity,






hydrolyzing O-glycosyl compounds






(GO: 0004553), Biological Process:






carbohydrate metabolism (GO: 0005975)”












1820
BL03655
[1-31]
conserved hypothetical protein

YmdA


1839

[1-29]
hypothetical protein


1904
yoaO
[1-38]
YoaO

YoaO


1914
yoaW
[1-26]
YoaW

YoaW


1926

[1-38]
phage-like protein


1929
nucB
[1-34]
nuclease

NucB


1931
BL05188
[1-29]
Peptidoglycan-binding protein
Biological Process: cell wall
YneA






catabolism (GO: 0016998)


1946
yneN
[1-29]
putative thiol: disulfide
“Molecular Function: electron
YneN





interchange protein YneN
transporter activity






(GO: 0005489), Biological






Process: electron transport






(GO: 0006118)”


1983
bglC
[1-50]
“endo-1,4-beta-glucanase,
“Molecular Function: hydrolase
BglC





Glycoside hydrolase Family 5”
activity, hydrolyzing O-glycosyl






compounds (GO: 0004553),






Biological Process:






carbohydrate metabolism






(GO: 0005975),”











1990
ywoF
[1-22]
“Polysaccharide Lyase Family 9, YwoF”
YwoF












1995
BL00297
[1-27]
conserved hypothetical protein

YddR


2011
yjmF
[1-31]
Short-chain
“Biological Process: metabolism
YjmF





dehydrogenase/reductase SDR
(GO: 0008152), Molecular






Function: oxidoreductase






activity (GO: 0016491)”


2016
dctB
[1-29]
possible C4-dicarboxylate
“,Biological Process: transport
DctB





binding protein
(GO: 0006810), Cellular






Component: periplasmic space






(sensu Gram-negative Bacteria)






(GO: 0030288)”


2028
dacC
[1-29]
penicillin-binding protein (D-
“Molecular Function: serine
DacC





alanyl-D-alanine
carboxypeptidase activity





carboxypeptidase)
(GO: 0004185), Biological






Process: proteolysis and






peptidolysis (GO: 0006508)”


2060

[1-37]
hypothetical protein











2072
BL00899
[1-28]
“Spore germination B3 GerAC like, C-terminal”
YndF












2105

[1-33]
conserved hypothetical protein




2108
BL01303
[1-26]
Peptidoglycan-binding protein
“Biological Process: cell wall
YocH






catabolism






(GO: 0016998), Biological






Process: cell wall catabolism






(GO: 0016998)”


2123

[1-36]
hypothetical protein


2132
BL01404
[1-24]
hypothetical protein

YoqH


2152
yvgO
[1-25]
conserved protein YvgO

YvgO


2160
lytF
[1-27]
gamma-D-glutamate-meso-
“cell wall lytic activity, Biological
LytF





diaminopimelate muropeptidase
Process: cell wall catabolism





(major autolysin) (CWBP49′)
(GO: 0016998)”


2167
yoaJ
[1-26]
YoaJ

YoaJ


2177
ctpA
[1-37]
carboxy-terminal processing
“,Biological Process: proteolysis
CtpA





protease
and peptidolysis (GO: 0006508),






Molecular Function: serine-type






peptidase activity






(GO: 0008236)”


2180
yodJ
[1-31]
putative carboxypeptidase

YodJ


2207
ypmS
[1-34]
conserved protein YpmS

YpmS


2208
ypmR
[1-24]
conserved protein YpmR
Molecular Function: catalytic
YpmR






activity (GO: 0003824)


2209
ypmQ
[1-25]
conserved hypothetical YpmQ
Biological Process: electron
YpmQ






transport (GO: 0006118)











2218
ypjP
[1-30]
conserved hypothetical protein YpjP
YpjP












2266
ponA
[1-63]
“penicillin-binding
“involved in division septum
PonA





proteins, Glycosyl Transferase
formation,”





Family 51”


2271
aspB
[1-23]
aspartate aminotransferase
“Molecular Function:
AspB






transaminase activity






(GO: 0008483), Biological






Process: biosynthesis






(GO: 0009058)”


2272
ypmB
[1-25]
conserved protein YpmB

YpmB


2315
BL02789
[1-29]
hypothetical protein

YphF


2328
sleB
[1-34]
spore cortex-lytic enzyme

SleB


2330
ansZA
[1-18]
putative
“Biological Process: amino acid
YccC





Asparaginase/glutaminase
metabolism






(GO: 0006520), Biological






Process: amino acid metabolism






(GO: 0006520)”


2334
BL02228
[1-27]
putative hydrolase
Molecular Function: hydrolase
YpbG






activity (GO: 0016787)











2344
yocH
[1-31]
putative peptidogycan hydrolase
“Biological Process: cell wall catabolism





YocH
(GO: 0016998), Biological Process: cell wall






catabolism (GO: 0016998)”












2347
BL00652
[1-32]
hypothetical protein

LytR


2348
yheN
[1-53]
“Carbohydrate Esterase Family
“Biological Process:
YheN





4, YheN”
carbohydrate metabolism






(GO: 0005975), Molecular






Function: hydrolase activity,






acting on carbon-nitrogen (but






not peptide) bonds






(GO: 0016810)”


2349

[1-29]
hypothetical protein


2360
dacB
[1-28]
D-alanyl-D-alanine
“required for spore cortex
DacB





carboxypeptidase (penicillin-
synthesis, Molecular Function:





binding protein 5*)
serine carboxypeptidase activity






(GO: 0004185), Biological






Process: proteolysis and






peptidolysis (GO: 0006508)”


2371
BL01892
[1-44]
conserved hypothetical protein

YpuD


2376
BL03279
[1-24]
conserved hypothetical protein

YpuA


2392
dacF
[1-33]
penicilin binding protein
“required for spore cortex
DacF





(putative D-alanyl-D-alanine
synthesis, Molecular Function:





carboxypeptidase)
serine carboxypeptidase activity






(GO: 0004185), Biological






Process: proteolysis and






peptidolysis (GO: 0006508)”











2400
BL01161
[1-28]
“Phosphotransferase system,
“Molecular Function: sugar porter activity





lactose/cellobiose-specific IIB
(GO: 0005351), Biological Process:





subunit”
phosphoenolpyruvate-dependent sugar






phosphotransferase system (GO: 0009401)”












2418
lip
[1-31]
lipase
Molecular Function: catalytic
Lip






activity (GO: 0003824)


2420
appAA
[1-32]
oligopeptide ABC transporter
“oligopeptide
AppA





(oligopeptide-binding protein)
transport, Molecular Function:






transporter activity






(GO: 0005215), Biological






Process: transport






(GO: 0006810)”


2435

[1-24]
hypothetical protein


2436

[1-29]
hypothetical protein


2438
BL00808
[1-38]
hypothetical protein

YmaC


2449

[1-35]
hypothetical protein


2450

[1-31]
conserved hyopothetical protein

SsuA











2456
BL01380
[1-28]
“Phosphotransferase system,
“Molecular Function: sugar porter activity





lactose/cellobiose-specific IIB
(GO: 0005351), Biological Process:





subunit”
phosphoenolpyruvate-dependent sugar






phosphotransferase system (GO: 0009401)”












2481
BL01514
[1-36]
Cell wall hydrolase/autolysin
“Molecular Function: N-
Yqil






acetylmuramoyl-L-alanine






amidase activity (GO: 0008745),






Biological Process:






peptidoglycan catabolism






(GO: 0009253)”


2482
yqiH
[1-33]
YqiH

YqiH


2484

[1-26]
hypothetical protein


2499
spoIIIAH
[1-32]
SpoIIIAH
“mutants block sporulation after
SpoIIIAH






engulfment,”


2505
spoIIIAB
[1-23]
SpoIIIAB
“mutants block sporulation after
SpoIIIAB






engulfment,”


2517
yqhL
[1-24]
conserved protein YqhL

YqhL


2518
opuAC
[1-31]
glycine betaine ABC transporter
“glycine betaine
OpuAC





(glycine betaine-binding protein)
transport, Molecular Function:






transporter activity






(GO: 0005215), Molecular






Function: binding






(GO: 0005488), Biological






Process: transport






(GO: 0006810)”











2528
tasA
[1-28]
translocation-dependent antimicrobial spore component
TasA












2530
yqxM
[1-44]
YqxM

YqxM


2531
yqzG
[1-24]
YqzG

YqzG


2533
comGG
[1-38]
probably part of the DNA
“required for exogenous DNA-
ComGG





transport machinery ComGG
binding,”


2546
yqgU
[1-28]
conserved protein YqgU

YqgU


2548
fhuD
[1-32]
ferrichrome ABC transporter
“Molecular Function: iron ion
FhuD





(ferrichrome-binding protein)
transporter activity






(GO: 0005381), Biological






Process: high affinity iron ion






transport (GO: 0006827)”


2560
yqzC
[1-33]
conserved protein YqzC

YqzC


2565
pstS
[1-33]
phosphate ABC transporter
“involved in high-affinity
PstS





(binding protein)
phosphate uptake, Molecular






Function: transporter activity






(GO: 0005215), Biological






Process: transport






(GO: 0006810)”


2566
pbpA
[1-44]
penicillin-binding protein 2A
“involved in the synthesis of
PbpA






peptidoglycan associated with






cell wall elongation in spore






outgrowth, Molecular Function:






penicillin binding (GO: 0008658)”


2571
yqfZ
[1-40]
conserved protein YqfZ


2616
BL01411
[1-37]
conserved hypothetical protein

YqfA


2632
yqxA
[1-29]
YqxA

YqxA


2633
spoIIP
[1-55]
SpoIIP
“required for dissolution of the
SpoIIP






septal cell wall,”


2652
BL02075
[1-36]
putative lipoprotein
Molecular Function: catalytic
YqeF






activity (GO: 0003824)


2697
appAB
[1-36]
oligopeptide ABC transporter
“oligopeptide
AppA





(oligopeptide-binding protein)
transport, Molecular Function:






transporter activity






(GO: 0005215), Biological






Process: transport






(GO: 0006810)”


2706

[1-32]
putative phosphoesterase


2707
BL02428
[1-27]
carboxylesterase family
Molecular Function: catalytic
PnbA






activity (GO: 0003824)


2710
BL03118
[1-28]
hypothetical protein

YndA


2712
sacC
[1-24]
Glycoside Hydrolase Family 32
“Molecular Function: hydrolase
SacC






activity, hydrolyzing O-glycosyl






compounds (GO: 0004553),






Biological Process:






carbohydrate metabolism






(GO: 0005975)”


2729
BL00468
[1-33]
Periplasmic iron-binding protein
“Molecular Function: iron ion
YxeB






transporter activity






(GO: 0005381), Biological






Process: high affinity iron ion






transport (GO: 0006827)”


2745
yrrS
[1-50]
conserved protein YrrS

YrrS


2752
yrrL
[1-44]
conserved protein YrrL

YrrL











2771
yrvJ
[1-29]
N-acetylmuramoyl-L-alanine amidase YrvJ
YrvJ












2793
ymaC
[1-24]
phage-related protein YmaC

YmaC


2797
coxA
[1-27]
spore cortex protein

CoxA


2801
nadB
[1-37]
L-aspartate oxidase
“required for NAD biosynthesis,”
NadB


2818
mreC
[1-33]
cell-shape determining protein
“,Biological Process: regulation
MreC






of cell shape (GO: 0008360)”


2866

[1-28]
hypothetical protein


2872
gerM
[1-34]
spore germination protein GerM
“germination (cortex hydrolysis)
GerM






and sporulation (stage II,






multiple polar septa),”


2898

[1-33]
hypothetical protein


2911
araN
[1-32]
sugar-binding protein
“L-arabinose transport, Molecular
AraN






Function: transporter activity






(GO: 0005215), Biological






Process: transport






(GO: 0006810)”


2916
abnAB
[1-28]
Glycoside Hydrolase Family 43
“degradation of plant cell wall
AbnA






polysaccharide, Molecular






Function: hydrolase activity,






hydrolyzing O-glycosyl






compounds (GO: 0004553),






Biological Process:






carbohydrate metabolism






(GO: 0005975)”











2939
pelB
[1-31]
“pectate lyase, Polysaccharide Lyase Family 1”
PelB












3005
ytxE
[1-51]
YtxE
Cellular Component: outer
YtxE






membrane (sensu Gram-






negative Bacteria)






(GO: 0009279)


3049
ytcQ
[1-30]
putative multiple sugar transport
“Molecular Function: transporter
YtcQ





system substrate-binding protein
activity (GO: 0005215),





YtcQ
Biological Process: transport






(GO: 0006810)”


3059
BL00013
[1-30]
putative lipoprotein

YusA











3081
ytlA
[1-33]
putative sulfonate transport system substrate-binding protein YtlA
YtlA












3088
ytkA
[1-29]
conserved protein YtkA

YtkA


3093
BL05310
[1-29]
Periplasmic solute binding
“Molecular Function: binding
YcdH





protein
(GO: 0005488), Cellular






Component: periplasmic space






(sensu Gram-negative Bacteria)






(GO: 0030288)”











3217

[1-22]
hypothetical protein
Molecular Function: catalytic activity






(GO: 0003824)












3235
yuiC
[1-34]
conserved protein YuiC

YuiC


3264
yutC
[1-24]
conserved protein YutC

YutC


3266
BL02121
[1-22]
putative metallopeptidase
“Molecular Function:
YunA






metalloendopeptidase activity






(GO: 0004222), Biological






Process: proteolysis and






peptidolysis (GO: 0006508)”


3267

[1-32]
hypothetical protein


3269
yunB
[1-41]
conserved protein YunB

YunB


3284
yurYA
[1-40]
extracellular ribonuclease
Molecular Function: nuclease
YurI






activity (GO: 0004518)











3298
yusA
[1-28]
putative ABC transport system substrate-binding protein YusA
YusA












3318
yfiY
[1-29]
ABC transport system substrate-
“Molecular Function: iron ion
YfiY





binding protein
transporter activity






(GO: 0005381), Biological






Process: high affinity iron ion






transport (GO: 0006827)”


3319
yusW
[1-28]
YusW

YusW


3333
gerAC
[1-25]
spore germination protein A3
“germination response to L-
GerAC





precursor
alanine and related amino acids






(earliest stage),”


3338
yvqG
[1-39]
conserved protein YvqG
“Biological Process: metabolism
YvqG






(GO: 0008152), Molecular






Function: oxidoreductase






activity (GO: 0016491)”


3349
yvrC
[1-37]
putative iron transport system
“Molecular Function: iron ion
YvrC





substrate-binding protein YvrC
transporter activity






(GO: 0005381), Biological






Process: high affinity iron ion






transport (GO: 0006827)”


3380
BL02684
[1-27]
putative Extracellular solute-
Molecular Function: transporter
YvfK





binding protein
activity (GO: 0005215)











3384
BL02680
[1-31]
putative Glycoside Hydrolase Family 3
YbbD












3387
mntA
[1-28]
manganese transport system
“Molecular Function: binding
YcdH





substrate-binding protein
(GO: 0005488), Cellular






Component: periplasmic space






(sensu Gram-negative Bacteria)






(GO: 0030288)”


3429

[1-33]
hypothetical protein


3465

[1-32]
hypothetical protein


3492
opuCC
[1-32]
glycine betaine/carnitine/choline
“high affinity transport of glycine
OpuCC





ABC transporter
betaine, carnitine, and





(osmoprotectant-binding protein)
choline, Molecular Function:






transporter activity






(GO: 0005215), Molecular






Function: binding






(GO: 0005488), Biological






Process: transport






(GO: 0006810)”


3499
BL03470
[1-29]
“Periplasmic binding
“Molecular Function: iron ion
YfiY





protein, putative iron transporter”
transporter activity






(GO: 0005381), Biological






Process: high affinity iron ion






transport (GO: 0006827)”


3510

[1-26]
hypothetical protein


3547
sacB
[1-30]
“levansucrase, Glycoside
“Molecular Function: hydrolase
SacB





Hydrolase Family 68”
activity, hydrolyzing O-glycosyl






compounds (GO: 0004553),






Biological Process: sugar






utilization (GO: 0007587)”











3548
levB
[1-33]
“endolevanase, Glycoside Hydrolase Family 32”
YveB











3559

[1-29]
putative ribonuclease
“Molecular Function: RNA binding






(GO: 0003723), Molecular Function:






endoribonuclease activity (GO: 0004521)”












3578
yvpB
[1-39]
putative cysteine protease YvpB
“Molecular Function: cysteine-
YvpB






type endopeptidase activity






(GO: 0004197), Biological






Process: proteolysis and






peptidolysis (GO: 0006508)”











3579
yvpA
[1-29]
Polysaccharide Lyase Family 3
YvpA












3587
yvnB
[1-29]
YvnB

YvnB


3600
ctpB
[1-37]
“Peptidase S41A, C-terminal
“Biological Process: proteolysis
YvjB





protease”
and peptidolysis (GO: 0006508),






Molecular Function: serine-type






peptidase activity (GO: 0008236)”


3602
BL03388
[1-291]
“Spectrin repeat, Rudiment
“,”
YvcE





single hybrid motif”


3643
lytC
[1-14]
N-acetylmuramoyl-L-alanine
“involved in cell separation, cell
LytC





amidase (major autolysin)
wall turnover, antibiotic-induced





(CWBP49)
lysis, motility and general cell






lysis induced by sodium






azide, Molecular Function: N-






acetylmuramoyl-L-alanine






amidase activity (GO: 0008745),






Biological Process:






peptidoglycan catabolism











3644
lytB
[1-26]
modifier protein of major autolysin LytC (CWBP76)
LytB












3645
BL03297
[1-25]
membrane bound lipoprotein
involved in the secretion of
LytA






major autolysin


3646
lytR
[1-32]
membrane-bound protein
“attenuator role for lytABC and
LytR






lytR expression,”


3656
lytD
[1-29]
“N-acetylglucosaminidase
“involved in cell separation, cell
LytD





(major autolysin), Glycoside
wall turnover, antibiotic-induced





Hydrolase Family 73”
lysis and motility, Molecular






Function: amidase activity






(GO: 0004040), Biological






Process: peptidoglycan






catabolism (GO: 0009253)”


3670
pgdS
[1-34]
gamma-DL-glutamyl hydrolase
“gamma-DL-glutamyl hydrolase
YwtD






cleaving the gamma-glutamyl






bond only between D- and L-






glutamic acids of PGA, DL-






endopeptidase II family”


3671
ywtC
[1-25]
YwtC











3672
pgsAA
[1-47]
poly-gamma-glutamate synthesis protein
YwtB












3680
rbsB
[1-28]
ribose ABC transporter (ribose-
“ribose transport,”
RbsB





binding protein)


3738
feuA
[1-28]
iron-binding protein
“component of iron-uptake
FeuA






system, Molecular Function: iron






ion transporter activity






(GO: 0005381), Biological






Process: high affinity iron ion






transport (GO: 0006827)”


3751
ywmD
[1-25]
YwmD

YwmD


3752
ywmC
[1-24]
YwmC

YwmC


3753
spoIID
[1-33]
SpoIID
“required for complete
SpoIID






dissolution of the asymmetric






septum,”


3755
ywmB
[1-30]
conserved protein YwmB

YwmB


3773
spoIIR
[1-29]
SpoIIR
required for processing of pro-
SpoIIR






sigma-E


3801
ywjE
[1-28]
putative Phospholipase
“Molecular Function: catalytic
YwjE






activity (GO: 0003824),






Biological Process: metabolism






(GO: 0008152)”


3808
BL03962
[1-41]
“Penicillin-binding protein,
“Molecular Function: penicillin
YwhE





Glycosyl Transferase Family 51”
binding (GO: 0008658),






Biological Process: cell wall






biosynthesis (sensu Bacteria)






(GO: 0009273)”


3812

[1-25]
hypothetical protein


3829
BL03904
[1-32]
hypothetical protein

YuaB


3849
vpr
[1-29]
extracellular serine protease
“Molecular Function: subtilase
Vpr






activity (GO: 0004289),






Biological Process: proteolysis






and peptidolysis (GO: 0006508)”


3906
ywaD
[1-31]
Putative aminopeptidase
“Biological Process: proteolysis
YwaD






and peptidolysis (GO: 0006508),






Molecular Function: peptidase






activity (GO: 0008233)”


3914
dltD
[1-27]
precusor DltD
D-alanine esterification of
DltD






lipoteichoic acid and wall






teichoic acid (D-alanine transfer






from undecaprenol-P to the






poly(glycerophosphate) chain of






LTA)











3920
licB
[1-24]
phosphotransferase system
“Molecular Function: sugar porter activity





(PTS) lichenan-specific enzyme
(GO: 0005351), Biological Process:





IIB component
phosphoenolpyruvate-dependent sugar






phosphotransferase system (GO: 0009401)”












3933
BL05372
[1-31]
hypothetical protein

YweA


3954
lanP
[1-25]
serine protease
“subtilase activity
IspA






(GO: 0004289), proteolysis and






peptidolysis (GO: 0006508),






lantibiotic leader peptide






processing”


3970
ansA
[1-29]
L-asparaginase
“Molecular Function:
AnsA






asparaginase activity






(GO: 0004067), Biological






Process: amino acid metabolism






(GO: 0006520)”


3977
BL02958
[1-25]
conserved hypothetical protein

YxeA


3978
yxkH
[1-28]
“Polysaccharide deacetylase,
“Biological Process:
YxkH





Carbohydrate Esterase Family
carbohydrate metabolism





4”
(GO: 0005975), Molecular






Function: hydrolase activity,






acting on carbon-nitrogen (but






not peptide) bonds






(GO: 0016810)”


3982
BL02963
[1-30]
Glycerophosphoryl diester
“Biological Process: glycerol
YhdW





phosphodiesterase
metabolism (GO: 0006071),






Molecular Function:






glycerophosphodiester






phosphodiesterase activity






(GO: 0008889)”


3983
BL05379
[1-28]
hypothetical protein


3996

[1-73]
hypothetical protein


4011
BL05383
[1-34]
hypothetical protein

YjeA











4016

[1-31]
hypothetical protein
Biological Process: electron transport






(GO: 0006118)












4046
yxiA
[1-27]
“Glycoside hydrolase, family 43”
“Molecular Function: hydrolase
YxiA






activity, hydrolyzing O-glycosyl






compounds (GO: 0004553),






Biological Process:






carbohydrate metabolism






(GO: 0005975)”


4058
ydaJ
[1-29]
putative Glycoside transferase

YdaJ


4080
glpQ
[1-29]
glycerophosphoryl diester
“hydrolysis of deacylated
GlpQ





phosphodiesterase
phospholipids, Biological






Process: glycerol metabolism






(GO: 0006071), Molecular






Function: glycerophosphodiester






phosphodiesterase activity






(GO: 0008889)”


4099
yxeA
[1-34]
conserved hypothetical protein











4103
yvfO
[1-28]
Glyccosyl Hydrolase Family 53
YvfO












4107
cycB
[1-32]
“putative extracellular solute-
“Molecular Function: transporter
YvfK





binding protein, family 1 CycB”
activity (GO: 0005215),






Biological Process: transport






(GO: 0006810)”


4121

[1-28]
hypothetical protein


4128

[1-34]
hypothetical protein


4130

[1-42]
hypothetical protein


4158
yycH
[1-36]
conserved hypothetical YycH

YycH
















TABLE 4







Codon Usage Table for Chromosome













# Codon
Amino acid
Fract
/1000
Number

















GCA
A
0.230
18.394
22495



GCC
A
0.272
21.724
26567



GCG
A
0.301
24.082
29451



GCT
A
0.197
15.766
19281



TGC
C
0.662
5.113
6253



TGT
C
0.338
2.613
3195



GAC
D
0.432
22.129
27063



GAT
D
0.568
29.114
35605



GAA
E
0.704
51.721
63253



GAG
E
0.296
21.797
26657



TTC
F
0.354
16.034
19609



TTT
F
0.646
29.223
35738



GGA
G
0.316
22.644
27693



GGC
G
0.374
26.825
32806



GGG
G
0.171
12.287
15026



GGT
G
0.139
9.957
12177



CAC
H
0.386
8.412
10288



CAT
H
0.614
13.369
16350



ATA
I
0.101
7.217
8826



ATC
I
0.487
34.741
42487



ATT
I
0.412
29.376
35926



AAA
K
0.693
49.125
60078



AAG
K
0.307
21.739
26586



CTA
L
0.032
3.106
3798



CTC
L
0.152
14.848
18158



CTG
L
0.263
25.592
31298



CTT
L
0.229
22.330
27309



TTA
L
0.143
13.920
17023



TTG
L
0.181
17.639
21572



ATG
M
1.000
26.541
32458



AAC
N
0.505
19.159
23431



AAT
N
0.495
18.780
22967



CCA
P
0.112
4.189



CCC
P
0.120
4.477
5475



CCG
P
0.520
19.405
23731



CCT
P
0.248
9.236
11295



CAA
Q
0.465
16.656
20369



CAG
Q
0.535
19.179
23455



AGA
R
0.218
9.611
11754



AGG
R
0.146
6.448
7886



CGA
R
0.074
3.275
4005



CGC
R
0.253
11.173
13664



CGG
R
0.194
8.538
10441



CGT
R
0.114
5.044
6168



AGC
S
0.263
15.893
19436



AGT
S
0.063
3.820
4672



TCA
S
0.202
12.173
14887



TCC
S
0.154
9.287
11357



TCG
S
0.171
10.289
12583



TCT
S
0.147
8.865
10841



ACA
T
0.327
16.969
20752



ACC
T
0.188
9.765
11942



ACG
T
0.373
19.404
23730



ACT
T
0.112
5.833
7134



GTA
V
0.144
9.710
11875



GTC
V
0.361
24.342
29769



GTG
V
0.238
16.042
19619



GTT
V
0.257
17.354
21223



TGG
W
1.000
10.303
12600



TAC
Y
0.398
13.468
16471



TAT
Y
0.602
20.348
24885



TAA
*
0.551
1.962
2400



TAG
*
0.157
0.558
682



TGA
*
0.292
1.041
1273

















TABLE 5







Signal Peptide Codon Usage Table













Codon
Amino acid
Fract
/1000
Number

















GCA
A
0.294
42.315
106



GCC
A
0.219
31.537
79



GCG
A
0.242
34.731
87



GCT
A
0.244
35.13
88



TGC
C
0.543
9.98
25



TGT
C
0.457
8.383
21



GAC
D
0.231
2.395
6



GAT
D
0.769
7.984
20



GAA
E
0.784
15.968
40



GAG
E
0.216
4.391
11



TTC
F
0.335
21.956
55



TTT
F
0.665
43.513
109



GGA
G
0.341
22.355
56



GGC
G
0.262
17.166
43



GGG
G
0.183
11.976
30



GGT
G
0.213
13.972
35



CAC
H
0.316
2.395
6



CAT
H
0.684
5.19
13



ATA
I
0.142
9.98
25



ATC
I
0.466
32.735
82



ATT
I
0.392
27.545
69



AAA
K
0.767
61.876
155



AAG
K
0.233
18.762
47



CTA
L
0.05
7.186
18



CTC
L
0.098
13.972
35



CTG
L
0.235
33.533
84



CTT
L
0.16
22.754
57



TTA
L
0.221
31.537
79



TTG
L
0.235
33.533
84



ATG
M
1
51.497
129



AAC
N
0.482
10.778
27



AAT
N
0.518
11.577
29



CCA
P
0.164
4.79
12



CCC
P
0.192
5.589
14



CCG
P
0.452
13.174
33



CCT
P
0.192
5.589
14



CAA
Q
0.475
11.577
29



CAG
Q
0.525
12.774
32



AGA
R
0.333
9.98
25



AGG
R
0.08
2.395
6



CGA
R
0.107
3.194
8



CGC
R
0.16
4.79
12



CGG
R
0.173
5.19
13



CGT
R
0.147
4.391
11



AGC
S
0.171
14.77
37



AGT
S
0.093
7.984
20



TCA
S
0.241
20.758
52



TCC
S
0.148
12.774
32



TCG
S
0.134
11.577
29



TCT
S
0.213
18.363
46



ACA
T
0.262
11.178
28



ACC
T
0.234
9.98
25



ACG
T
0.327
13.972
35



ACT
T
0.178
7.585
19



GTA
V
0.216
14.371
36



GTC
V
0.186
12.375
31



GTG
V
0.335
22.355
56



GTT
V
0.263
17.565
44



TGG
W
1
7.585
19



TAC
Y
0.405
5.988
15



TAT
Y
0.595
8.782
22



TAA
*
0
0
0



TAG
*
0
0
0



TGA
*
0
0
0










Example 6
Identification of Genes Induced by Glucose Limitation Using DNA Microarrays
Bacillus licheniformis ATCC 14580 was grown in duplicate shake flasks containing 100 ml of Spizizen I medium (Anagnostopoulos and Spizizen, 1961, Journal of Bacteriology 81: 741-746). The inocula for the shake flasks were obtained from 10 ml of mid-log cells (approximately 80-90 Klett units) and a 0 hour total RNA sample was extracted from 30 ml of the mid-log cells, as described below. Total cellular RNA was also purified from 10 ml of the Spizizen I shake flasks that were sampled at 2, 4, 6 and 8 hours post inoculum. All of the cell samples were mixed with two volumes of RNAProtect RNA stabilizing reagent (QIAGEN, Inc., Valencia, Calif.) for 5 minutes. The cells were subsequently pelleted at 2800×g, 4° C. for 10 minutes, the supernatant was discarded, the cell pellets were frozen at −80° C., and cellular RNA was purified using a FastRNA Pro Blue kit (QBiogene, Carlsbad, Calif.) using the protocol supplied by the manufacturer. The frozen cell pellets from each sample were resuspended in 2 ml of RNAPro solution provided in the FastRNA Pro Blue kit and RNA was obtained from two lysis matrix B vials.
Sixteen replicate cDNA targets for the 0 time point and two technical replicate cDNA targets for each of the rest of the time points were prepared and hybridized to Bacillus licheniformis DNA microarrays prepared as described by Berka et al., 2002, Molecular Microbiology 43: 1331-1345. Dye swap hybridizations using the zero time point as reference were prepared as described in Hu et al. [In G. Parmigiani, E. S. Garrett, R. A Irizarry, and S. L Zeger (eds.) The Analysis of Gene Expression Data, pp. 318-319, Springer-Verlag, New York (2003)].
The arrays were scanned with an Axon 4000B scanner and formatted for analysis with GenePix Pro version 5.0 (Axon Instruments, Inc. Redwood City, Calif.). The fluorescence intensity data from GenePix was imported directly into S-Plus ArrayAnalyzer version 2 software (Insightful Corporation, Seattle, Wash.) for statistical analysis. The raw intensity data were normalized using the lowess (locally weighted regression) function provided in S-Plus ArrayAnalyzer software, and the genes that were differentially expressed at each time point relative to the time zero reference were identified by employing a multiple comparison t-test with Bonferroni correction as outlined in the user's guide to S-Plus ArrayAnalyzer software (Insightful Corp., Seattle, Wash.). The family-wise error rate (FWER) was set at 0.1. The list of genes at each time point that passed these statistical criteria were used to query a pathway-genome database established using Pathway Tools Suite software (Karp et al., 2002, Bioinformatics 18 suppl. 1: S225-S232), and the gene expression profiles were painted onto a schematic of metabolic pathways. In doing so, those pathways that were significantly altered at each time point were identified.
As a result of this analysis the following observations were made: (1) Early in the cultures, genes encoding enzymes of the glycolytic pathway were slightly induced reflecting the change from partially depleted glucose levels in the inoculum culture to ample glucose in the shake flask medium; (2) As glucose was progressively depleted in the shake flask culture (times 6 and 8 hours), genes encoding glycolytic pathway enzymes were down-regulated; (3) genes that encode enzymes for phenylalanine, tryptophan, histidine, and arginine biosynthetic pathways were up-regulated during stationary phase suggesting that cells had exhausted critical amino acids, and they were turning on the genes for pathways to synthesize more of these essential nutrients; (4) as cells entered stationary phase, cell division, DNA replication, and ribosome production all decreased to a low level. This was reflected in the shut-down of purine and pyrimidine biosynthetic pathways and down-regulation of genes encoding many ribosomal proteins.
SEQUENCE LISTINGS
Incorporated herein by reference are 2 copies of the Sequence Listing on compact disk. Copy 1 is done on a Intel x86 machine format, in Windows XP operating system compatibility, there is one file saved as 10588.200, and is 19,455 kb, and created on Nov. 4, 2004. Copy 2 is identical to Copy 1. The content of the attached compact disks are the same and includes no new matter.
The invention described and claimed herein is not to be limited in scope by the specific aspects herein disclosed, since these aspects are intended as illustrations of several aspects of the invention. Any equivalent aspects are intended to be within the scope of this invention. Indeed, various modifications of the invention in addition to those shown and described herein will become apparent to those skilled in the art from the foregoing description. Such modifications are also intended to fall within the scope of the appended claims. In the case of conflict, the present disclosure including definitions will control.
Various references are cited herein, the disclosures of which are incorporated by reference in their entireties.
SEQUENCE LISTINGS
This application contains 2 copies of the Sequence Listing on compact disk, which are incorporated herein by reference. Copy 1 is done on an Intel x86 machine format, in Windows XP operating system compatibility, there is one file saved as 10588200 SQ, and is 19,445 kb bytes, and created on May 3, 2005. Copy 2 is identical to Copy 1.

## Claims

1. An isolated polypeptide having cellulose 1,4-beta-cellobiosidase activity, selected from the group consisting of:
(a) a polypeptide having cellulose 1,4-beta-cellobiosidase activity comprising an amino acid sequence having at least 95% identity with the amino acid sequence of SEQ ID NO: 5979; and
(b) a polypeptide having cellulose 1,4-beta-cellobiosidase activity encoded by a gene comprising a nucleotide sequence which hybridizes under high stringency conditions with the nucleotide sequence of SEQ ID NO: 1782, or the full-length complementary strand thereof, wherein high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 μg/ml sheared and denatured salmon sperm DNA, and 50% formamide, and washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 65° C.

2. The polypeptide having cellulose 1,4-beta-cellobiosidase activity of claim 1, which comprises an amino acid sequence having at least 95% identity with the amino acid sequence of SEQ ID NO: 5979.

3. The polypeptide having cellulose 1,4-beta-cellobiosidase activity of claim 1, which is encoded by a gene comprising a nucleotide sequence which hybridizes under high stringency conditions with the nucleotide sequence of SEQ ID NO: 1782, or the full-length complementary strand thereof, wherein high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 μg/ml sheared and denatured salmon sperm DNA, and 50% a formamide, and washing three times each for 15 minutes using 2×SSC, 0.2% a SDS at 65° C.

4. The polypeptide having cellulose 2,4-beta-cellobiosidase activity of claim 1, which is encoded by a Bacillus licheniformis ATCC 14580 gene.

5. The polypeptide having cellulose 1,4-beta-cellobiosidase activity of claim 2, which comprises an amino acid sequence having at least 97% a identity with the amino acid sequence of SEQ ID NO: 5979.

6. The polypeptide having cellulose 1,4-beta-cellobiosidase activity of claim 1, which comprises the amino acid sequence of SEQ ID NO: 5979.

7. The polypeptide having cellulose 1,4-beta-cellobiosidase activity of claim 1, which consists of the amino acid sequence of SEQ ID NO: 5979, or a fragment thereof which retains cellulose 1,4-beta-cellobiosidase activity.