UNITED STATES ENVIRONMENTAL PROTECTION 

AGENCY

WASHINGTON D.C., 20460

OFFICE OF 

PREVENTION, PESTICIDES AND 

TOXIC SUBSTANCES

PC Code: 129112 

DP Barcode: 361159 

Date: June 26, 2009 

MEMORANDUM 

SUBJECT: Trifloxystrobin Ecological Risk Review for a Rate Increase and Formulation 

Change for Use on Soybeans 

FROM: Michael Lowit, Ecologist 

 Mark Corbin, Senior Environmental Fate Scientist 

Environmental Risk Branch III 

 Environmental Fate and Effects Division (7507P) 

TO: Tony Kish 

Registration Division (RM 22) 

THRU: Dana Spatz 

Branch Chief, Environmental Risk Branch III 

 Environmental Fate and Effects Division (7507P) 

 

This memorandum summarizes the ecological risks associated with the proposed new use of 

trifloxystrobin as a fungicide on soybeans. The risk assessment considers the product Stratego®

731 Fungicide (EPA Reg. No. 264-RNOG). 

Risk Summary 

The Environmental Fate and Effects Division (EFED) has evaluated the new use request for a 

formulation change and rate increase for the use of trifloxystrobin on soybeans as a foliar spray 

(aerial and ground) and by chemigation. This risk assessment considers Stratego 731® Fungicide 

(EPA Reg. No. 264-RNOG) which contains both trifloxystrobin and prothioconazole. 

Prothioconazole was not considered in this assessment, and potential interactions (additive, 

synergistic, or inhibitory) between the two active ingredients were not considered. 

No new environmental fate or effects data have been submitted since the most recent ecological 

risk assessment for soybeans was conducted on December 19, 2005 (D318621, D318623). The 
2

current assessment relies on the data summaries and risk conclusions from the previous 

assessment. In order to avoid repetition of material previously provided, please refer to the 

previous assessment (D318621, D318623) for detailed information. 

The current request is for EFED to consider the impact of a formulation change from an 

emulsifiable concentrate (EC) evaluated previously to a soluble concentrate (SC) of the new 

product. Because both formulations will behave essentially the same once applied in the field, the 

data and summaries from the previous assessment cover this new use. 

In addition, the new request is for a rate increase to 0.114 lbs ai/acre applied three times per year 

with 10 day intervals between applications which is higher than the previously assessed rate of 

0.082 lbs ai/acre with three applications per year and 10 day intervals. EFED’s assessment of the 

new use rate indicates that no taxa exceed the Agency’s LOC similar to the conclusion from the 

2005 risk assessment for soybean use (D318621, D318623). However, previous assessments of 

other uses of trifloxystrobin have shown exceedences of the Agency’s LOC. 

Trifloxystrobin has been previously evaluated by EFED for use on numerous commodities 

including pome fruits, grapes, cucurbits, peanuts, and turf (New Chemical Review, 

D244219, D245281, D254206, D254285, D254286); almonds, fruiting vegetables, hops, 

potatoes, sugar beets, and wheat (D254215, D254220, D254222); root vegetable subgroup 

1B (except beet and radish) and leaf petiole subgroup 4B (D286427); barley, oats, sweet 

corn, and soybeans (D315580, D314187, D318621, D318623); grass grown for seed 

(D332979, D332982); asparagus, tropical crops, radish, root vegetables, and strawberries 

(D331915, D339548, D348876). Detailed summaries of these assessments can be found 

elsewhere (D309489, D331915). The proposed application rates for the new use on 

soybeans is less than several of the rates (turf, cucurbits, grapes, grass grown for seed, 

asparagus, tropical crops, radish, root vegetables, leaf petiole, almonds, fruiting 

vegetables, hops, and potatoes) previously considered. 

Based on previous reviews, EFED concluded that some uses are expected to present risk 

on an acute basis to non-target aquatic organisms (fish and invertebrates); including 

Federally-listed endangered and threatened species. Those uses include turf, cucurbits, 

grasses grown for seed, tropical crops, root vegetables, leaf petiole, almonds, fruiting 

vegetables, hops, and potatoes. 

The key uncertainties identified in the 2005 risk assessment (D318621, D318623) are applicable 

to the current assessment: 

• Risk to endangered terrestrial plants could not be assessed due to the limitations of the 

submitted Tier I toxicity studies. Therefore, risk is presumed because suitable Tier II 

plant toxicity studies are not available for consideration. 

• In addition, another strobilurin fungicide is highly toxic to some orchard fruits, including 

apples. Data on trifloxystrobin toxicity to apples or other orchard fruits were not 

submitted. Therefore, some risk to apples and other orchard fruits is presumed. 
3

• A chronic estuarine/marine invertebrate toxicity study has not been submitted. The mysid 

shrimp NOAEC of 0.96 ppb was estimated using an acute to chronic ratio derived from 

daphnid studies. If the acute to chronic ratio is not conserved across species, then risk 

could be over- or under-estimated. 

• A suitable aerobic aquatic metabolism study (162-4) has not been submitted; therefore, 

there remains a degree of uncertainty as to the persistence and dissipation of 

trifloxystrobin in aquatic environments. 

• Stratego 731® Fungicide contains both trifloxystrobin and prothioconazole. Potential 

interactions (additive, synergistic, or inhibitory) between the two active ingredients could 

result in an under- or over-estimation of the risk based on trifloxystrobin alone. 

Analysis 

Because the rates for the new use are higher than those previously assessed for soybeans and 

because EFED’s aquatic and terrestrial exposure models have been updated since the 2005 

assessment an evaluation of the impact of the rate change on exposure has been completed. 

These revised EECs were then compared to the toxicity data presented in the 2005 assessment 

(D318621, D318623) (Attachment 1). The previously submitted data were not re-evaluated. 

Table 1 summarizes the previous aquatic EECs relative to the new EECs. 

Table 1 Summary of Previous and Current Surface Water EECs 1, 2

Scenario Use Application Rate/

# Applications/ 

Interval 

Peak (ug/L) 21 day 

(ug/L) 

60 day 

(ug/L) 

Previous Assessment 

MS soybean Soybean 0.082 lbs ai/A 

3 applications 

10 days 

0.28 0.07 0.03 

Current Assessment

MS soybean Soybean 0.114 lb ai/A 

3 applications 

10 days 

0.36 0.15 0.09 

1

 The previously summarized environmental fate data and PRZM model input/output for the 

current EECs are included in Attachments 2 and 3, respectively. 

2

 Surface water EECs were modeled only for trifloxystrobin. 

 

The surface water EECs are based on aerial application and are protective of foliar ground 

applications. The groundwater EEC (0.00007 ug/L) for the proposed use is much lower than the 

surface water EECs (Table 1), thus the estimated surface water EECs are also protective of 

chemigation applications. SCI-GROW (v2.3) was used to estimate the groundwater 

concentration using input values summarized in Attachment 2. 
4

Comparison of these updated aquatic EECs with the previously summarized toxicity data indicate 

that none of the Agency’s levels of concern for aquatic species is exceeded. The revised aquatic 

risk quotients for the increased rate are summarized in Attachment 4. 

Similarly, EFED has evaluated the impact of the rate increase on terrestrial exposure. The rate is 

approximately 40% higher than the previously assessed rate and inputting the new rate in T-REX 

(v1.4.1) yields terrestrial EECs that are higher by a similar magnitude. 

Tables 2 and 3 summarize the terrestrial EECs for the proposed new use. Input values for 

exposure estimates obtained from T-REX simulations of trifloxystrobin are as follows: 

application rate = 0.114 lb ai/A, number of applications = 3, application interval = 10 days, 

default foliar dissipation half-life = 35 days (EFED guidance). 

Table 2 Dietary-based Terrestrial Food Item Residue Estimates for Proposed Use

Food Item Avian and Mammal Dietary-based EECs (ppm) 

Short Grass 68.22 

Tall Grass 31.27 

Broadleaf plants/sm Insects 38.37 

Fruits/pods/seeds/lg insects 4.26 

Table 3 Dose-based Terrestrial Food Item Residue Estimates for Proposed Use

Avian Classes and Body Weights 

(grams) 

Mammal Classes and Body 

Weights (grams)

small mid large small mid large 

Food Item

20 100 1000 15 35 1000 

Short Grass 77.691 44.30 19.84 65.04 44.95 10.42 

Tall Grass 35.61 20.31 9.09 29.81 20.60 4.78 

Broadleaf plants/sm 

Insects 43.70 24.92 11.16 36.58 25.28 5.86 

Fruits/pods/seeds/lg 

insects 4.86 2.77 1.24 4.06 2.81 0.65 

1

 Dose-based EEC (mg/kg-bw) 

Comparison with toxicity data summarized in the previous assessment indicates that although 

predicted terrestrial EECs will be roughly 40% higher than those in the previous assessment, no 

exceedances of the Agency’s LOC for birds and mammals are predicted. A summary of the 

revised terrestrial RQs for the increased rate are included in Attachment 5. Typically, acute 

terrestrial animal RQs are not calculated when definitive toxicity endpoints are not available as is 

the case for trifloxystrobin (i.e., avian LD50 > 2000 mg/kg-bw; avian LC50 > 5050 mg/kg-feed, 

mammal LD50 > 5000 mg/kg-bw). Nonetheless, if acute RQs were calculated using these data, all 

avian and mammal acute RQs would be less than the Agency’s LOC. 

Finally, trifloxystrobin did not cause >25% inhibition in tier I terrestrial plant toxicity studies at 

0.249 lbs ai/Acre, which is above the maximum proposed application rate being assessed in this 

evaluation (0.114 lbs ai/Acre). Therefore, Tier II studies are not required. However, because 
5

inhibition was observed, and the data from a Tier I study are not sufficient to allow for calculation 

of a slope, an EC05, or a NOAEC, risk to endangered plant species cannot be assessed and, 

therefore, cannot be precluded. 
6

Attachment 1 

Ecological Effects Characterization 

A summary of trifloxystrobin toxicity data is presented below. A full discussion of the available 

data is included in the New Chemical Review (D254286) and new use reviews (D254215, -20, -

22, and D309489). No additional toxicity data have been submitted since the last assessment was 

completed in 2008 (D331915). Previously submitted data were not re-evaluated. Overall, 

trifloxystrobin is practically non-toxic to mammals and birds on an acute oral basis. 

Trifloxystrobin was not a reproductive toxicant in mammals or birds under the conditions of the 

submitted studies. In contrast, trifloxystrobin is highly toxic to fish and aquatic invertebrates. A 

summary of the key toxicity values that are used in this assessment is reported in Tables A and B 

below. 

Because the parent compound degrades rapidly to its major degradate, CGA-321113, which is 

relatively stable, chronic exposure in most aquatic environments is expected to occur mainly from 

this degradate. CGA-321113 has been studied in fish and aquatic invertebrates in acute studies 

and is practically non-toxic to these surrogate species on an acute basis. The degradate is also 

considerably less toxic than trifloxystrobin to daphnids on a chronic basis. Therefore, it is 

anticipated that risk quotients based on chronic studies using trifloxystrobin would be protective 

of aquatic organisms exposed to CGA-321113 on a chronic basis. The available data do not 

allow for a comparison of the relative toxicities of trifloxystrobin and the major degradate in 

terrestrial organisms. 

Table A. Terrestrial Toxicity Profile for Trifloxystrobin

Test Type Value Used in Risk 

Assessment 

Comments MRID 

Rat acute oral LD50 >5000 mg/kg-bw Acceptable study. 44496622 

Rat 2-generation 

reproduction 

NOAEL 1500 

mg/kg-feed 

Acceptable study. No treatment-related effects 

were observed at any concentration tested (up 

to 1500 ppm feed). 

44496710 

Bird acute oral LD50 

(Mallard Duck, Bobwhite 

Quail) 

LD50: >2000 - 

>2250 mg/kg-bw 

Acceptable studies. No mortality or treatmentrelated toxic effects occurred in either species. 44496606 

44496605 

Bird dietary LC50 (Mallard 

duck and Bobwhite quail) 

>5050 mg/kg-feed Acceptable study. No mortality or treatmentrelated toxic effects occurred in either species. 44496607 

44496608 

Reproductive Toxicity 

NOAEL (Bobwhite) 

320 mg/kg-feed Acceptable study. No treatment-related effects 

were observed at any concentration tested (up 

to 320 ppm feed). 

44496609 

Reproductive Toxicity 

NOAEL (Mallard duck) 

500 mg/kg-feed Acceptable study. No treatment-related effects 

were observed at any concentration tested (up 

44496610
7

Test Type Value Used in Risk 

Assessment 

Comments MRID 

to 500 ppm feed). 

Honey Bee Contact LD50 >200 ug/bee Acceptable study. No mortality was observed. 44496726 

Terrestrial Plants (Tier I) None used. EC25 is 

greater than the 

highest 

concentration tested 

(0.25 lbs a.i./Acre) 

Supplemental Study. The study is acceptable; 

however, it does not allow for an assessment of 

risk to endangered species because a 13% 

effect was observed in cabbage. The data do 

not allow for derivation of a NOAEC or an 

EC05. 

44496723 

Table B. Aquatic Toxicity Profile for Trifloxystrobin

Test Type Value Used in Risk 

Assessment 

Comments MRID 

Rainbow Trout LC50 

Degradate CGA-321113 

Bluegill Sunfish LC50 

14 ppb 

>106,000 ppb 

54 ppb 

Acceptable study 

Supplemental study (insufficient number of test 

organisms) 

Acceptable study 

44502803 

44527502 

44502802 

Daphnia magna EC50 

Degradate CGA-321113 

25 ppb 

>95,000 ppb 

Acceptable studies 

Acceptable studies 

44496611 

44527503 

Sheepshead minnow LC50 78 ppb Acceptable study 44496612 

Oyster shell deposition 

EC50 

29 ppb Acceptable study 44496613 

Mysid shrimp LC50 8.6 ppb Acceptable study 44496614 

Mysid shrimp NOAEC 0.96 ppb No study submitted. The estuarine/marine 

chronic NOAEC was estimated using acute to 

chronic ratios (ACRs) derived from daphnid 

acute (EC50=25 ppb) and chronic (NOAEC = 

2.8 ppb) toxicity values. The ACR was 8.9. 

Therefore, the estimated NOAEC in mysid 

shrimp would be 8.6/8.9 = 0.96 ppb. 

Not 

applicable 

Rainbow Trout Early Life 

Stage 

NOAEC: 4.3 ppb Acceptable study. The LOAEC was 7.7 ppb 

based on reduced survival. 

44502804 

Daphnia magna life cycle 

Degradate CGA-321113 

NOAEC: 2.8 ppb 

NOAEC: 3,200 ppb 

Acceptable study. The LOAEC was 5.9 ppb 

based on reduced survival and reproduction. 

Supplemental study. Water parameter 

measurements and growth endpoints of weight 

and length were not measured 

44496615 

44869701 

Green algae 

Degradate CGA-321113 

EC50: 37 ppb 

EC50: 78,800 ppb 

Acceptable study. The NOAEC was 10 ppb. 

Supplemental study. Test duration was only 72 

hours; a definitive EC50 was not determined 

44496724 

44527504 
8

Test Type Value Used in Risk 

Assessment 

Comments MRID 

Duckweed (Lemna gibba) EC50: >1930 ppb Acceptable study. The NOAEC was 410 ppb. 44496725 
9

Attachment 2 

Summary of Environmental Fate Data and Model Input Parameters for Trifloxystrobin 

Parameter Value Modeling Input Value Source 

Molecular Formula C20H19F3N2O4 DP Barcode D254920 

Molecular Weight 408.4 408.4 DP Barcode D254920 

Solubility @ 20 °

C 0.61 mg/L @ pH7 0.61 mg/L DP Barcode D254920 

Vapor Pressure 3.14e-06 Pa @ 25 °

C 

2.55e-08 mm Hg @ 20 °

C 

DP Barcode D254920 

Hydrolysis Half-life 55.2 days @ pH5 

0.83 days @ pH 7,9 

55.2 days 

0.83 days 

MRID 44496727 

Aquatic Photolysis Halflife 14.8 days @pH 5 14.8 days MRID 44496727 

Photolysis on Soil Halflife 2.1 days 2.1 days MRID 44496729 

Aerobic Soil 

Metabolism Half-life 

(T1⁄2) 

0.74, 0.41, and 0.91 days 

mean = 0.69 d, std = 0.25 d 

90th percent upper 

bound of mean = 

0.96 days 

(PRZM/EXAMS and 

SCI-GROW) 

MRID 44496732 

MRID 44496730 

MRID 44496731 

EFED Guidance 

Anaerobic Aquatic 

Metabolism Half-life 

0.72 days 2.16 days (3 x 0.72) MRID 44496733 

Aerobic Aquatic 

Metabolism Half-life 

0.60 days (supplemental) 

2 x aerobic soil metabolism 

(0.96 days) 

1.92 MRID 44496734 

EFED Guidance 

Organic Carbon 

Partition Coefficient 

(Koc) (mL/g) 

2354, 951, 3064, 3927, 9871, 

951 

r

2

 = (Kads vs org. matter) = 

0.03) 

Use mean Kads 

(PRZM/EXAMS) 

lowest Koc (SCIGROW) 

MRID 44496802 

Soil Partition Coefficient 

(Kads) (mL/g) 

(Kads) 

6.8, 7.7, 16.0, 59.4, 91.8, 33.2 

Mean = 35.82; Std = 33.8 

7.7 lowest non-sand 

mean Kads = 35.81

MRID 44496802 

Aerial Spray Drift 

Application Efficiency 

0.05 

0.95 

previous column EFED Input 

FEXTRA - foliar apps PRZM Foliar Extraction 0.5 (frac/cm rain) EFED Guidance 

PLDKRT - foliar apps PRZM Decay rate of Foliage 35 days EFED Guidance 

1

 The calculated mean Kads value of 26.9 mL/g used in the previous assessment of soybeans (D318621, 

D318623) was calculated incorrectly (the 59.4 value was incorrectly entered as 5.94). The correct mean 

value is 35.82 mL/g as indicated in the most recent new use assessment for trifloxystrobin (D331915). 
10

ATTACHMENT 3 

PRZM/EXAMS Input & Output 

 

MS soybean; 8/9/01 

"Yazoo County; MLRA 134; Metfile: W03940.dvf (old: Met131.met)," 

*** Record 3: 

 0.75 0.36 0 25 1 3 

*** Record 6 -- ERFLAG 

 4 

*** Record 7: 

 0.42 0.3 1 10 3 2 356.8 

*** Record 8 

 1 

*** Record 9 

 1 0.2 30 100 3 87 84 87 0 76 

*** Record 9a-e 

 1 27 

1604 2004 0105 0505 1605 0106 1606 0107 1607 0108 1608 0109 1609 0110 1510 1610 

.498 .575 .627 .654 .620 .484 .361 .220 .094 .109 .110 .046 .053 .040 .203 .239 

.014 .014 .014 .014 .014 .014 .014 .014 .014 .014 .014 .014 .014 .014 .014 .014 

 84 84 84 84 84 84 84 84 84 84 84 84 84 84 87 87 

0111 1611 0112 1612 0101 1601 0102 1602 0103 1603 0104 

.316 .394 .464 .524 .245 .276 .306 .337 .373 .418 .468 

.014 .014 .014 .014 .014 .014 .014 .014 .014 .014 .014 

 87 87 87 87 87 87 87 87 87 87 87 

*** Record 10 -- NCPDS, the number of cropping periods 

 30 

*** Record 11 

 160461 010961 101061 1 

 160462 010962 101062 1 

 160463 010963 101063 1 

 160464 010964 101064 1 

 160465 010965 101065 1 

 160466 010966 101066 1 

 160467 010967 101067 1 

 160468 010968 101068 1 

 160469 010969 101069 1 

 160470 010970 101070 1 

 160471 010971 101071 1 

 160472 010972 101072 1 

 160473 010973 101073 1 

 160474 010974 101074 1 

 160475 010975 101075 1 

 160476 010976 101076 1 

 160477 010977 101077 1 

 160478 010978 101078 1 

 160479 010979 101079 1 

 160480 010980 101080 1 
11

 160481 010981 101081 1 

 160482 010982 101082 1 

 160483 010983 101083 1 

 160484 010984 101084 1 

 160485 010985 101085 1 

 160486 010986 101086 1 

 160487 010987 101087 1 

 160488 010988 101088 1 

 160489 010989 101089 1 

 160490 010990 101090 1 

*** Record 12 -- PTITLE 

trifloxystrobin - 3 applications @ 0.128 0.128 0.128 kg/ha 

*** Record 13 

 90 1 0 0 

*** Record 15 -- PSTNAM 

trifloxystrobin 

*** Record 16 

 011061 0 2 4.0 0.128 .95 .05 

 111061 0 2 4.0 0.128 .95 .05 

 011161 0 2 4.0 0.128 .95 .05 

 011062 0 2 4.0 0.128 .95 .05 

 111062 0 2 4.0 0.128 .95 .05 

 011162 0 2 4.0 0.128 .95 .05 

 011063 0 2 4.0 0.128 .95 .05 

 111063 0 2 4.0 0.128 .95 .05 

 011163 0 2 4.0 0.128 .95 .05 

 011064 0 2 4.0 0.128 .95 .05 

 111064 0 2 4.0 0.128 .95 .05 

 011164 0 2 4.0 0.128 .95 .05 

 011065 0 2 4.0 0.128 .95 .05 

 111065 0 2 4.0 0.128 .95 .05 

 011165 0 2 4.0 0.128 .95 .05 

 011066 0 2 4.0 0.128 .95 .05 

 111066 0 2 4.0 0.128 .95 .05 

 011166 0 2 4.0 0.128 .95 .05 

 011067 0 2 4.0 0.128 .95 .05 

 111067 0 2 4.0 0.128 .95 .05 

 011167 0 2 4.0 0.128 .95 .05 

 011068 0 2 4.0 0.128 .95 .05 

 111068 0 2 4.0 0.128 .95 .05 

 011168 0 2 4.0 0.128 .95 .05 

 011069 0 2 4.0 0.128 .95 .05 

 111069 0 2 4.0 0.128 .95 .05 

 011169 0 2 4.0 0.128 .95 .05 

 011070 0 2 4.0 0.128 .95 .05 

 111070 0 2 4.0 0.128 .95 .05 

 011170 0 2 4.0 0.128 .95 .05 

 011071 0 2 4.0 0.128 .95 .05 

 111071 0 2 4.0 0.128 .95 .05 

 011171 0 2 4.0 0.128 .95 .05 
12

 011072 0 2 4.0 0.128 .95 .05 

 111072 0 2 4.0 0.128 .95 .05 

 011172 0 2 4.0 0.128 .95 .05 

 011073 0 2 4.0 0.128 .95 .05 

 111073 0 2 4.0 0.128 .95 .05 

 011173 0 2 4.0 0.128 .95 .05 

 011074 0 2 4.0 0.128 .95 .05 

 111074 0 2 4.0 0.128 .95 .05 

 011174 0 2 4.0 0.128 .95 .05 

 011075 0 2 4.0 0.128 .95 .05 

 111075 0 2 4.0 0.128 .95 .05 

 011175 0 2 4.0 0.128 .95 .05 

 011076 0 2 4.0 0.128 .95 .05 

 111076 0 2 4.0 0.128 .95 .05 

 011176 0 2 4.0 0.128 .95 .05 

 011077 0 2 4.0 0.128 .95 .05 

 111077 0 2 4.0 0.128 .95 .05 

 011177 0 2 4.0 0.128 .95 .05 

 011078 0 2 4.0 0.128 .95 .05 

 111078 0 2 4.0 0.128 .95 .05 

 011178 0 2 4.0 0.128 .95 .05 

 011079 0 2 4.0 0.128 .95 .05 

 111079 0 2 4.0 0.128 .95 .05 

 011179 0 2 4.0 0.128 .95 .05 

 011080 0 2 4.0 0.128 .95 .05 

 111080 0 2 4.0 0.128 .95 .05 

 011180 0 2 4.0 0.128 .95 .05 

 011081 0 2 4.0 0.128 .95 .05 

 111081 0 2 4.0 0.128 .95 .05 

 011181 0 2 4.0 0.128 .95 .05 

 011082 0 2 4.0 0.128 .95 .05 

 111082 0 2 4.0 0.128 .95 .05 

 011182 0 2 4.0 0.128 .95 .05 

 011083 0 2 4.0 0.128 .95 .05 

 111083 0 2 4.0 0.128 .95 .05 

 011183 0 2 4.0 0.128 .95 .05 

 011084 0 2 4.0 0.128 .95 .05 

 111084 0 2 4.0 0.128 .95 .05 

 011184 0 2 4.0 0.128 .95 .05 

 011085 0 2 4.0 0.128 .95 .05 

 111085 0 2 4.0 0.128 .95 .05 

 011185 0 2 4.0 0.128 .95 .05 

 011086 0 2 4.0 0.128 .95 .05 

 111086 0 2 4.0 0.128 .95 .05 

 011186 0 2 4.0 0.128 .95 .05 

 011087 0 2 4.0 0.128 .95 .05 

 111087 0 2 4.0 0.128 .95 .05 

 011187 0 2 4.0 0.128 .95 .05 

 011088 0 2 4.0 0.128 .95 .05 

 111088 0 2 4.0 0.128 .95 .05 
13

 011188 0 2 4.0 0.128 .95 .05 

 011089 0 2 4.0 0.128 .95 .05 

 111089 0 2 4.0 0.128 .95 .05 

 011189 0 2 4.0 0.128 .95 .05 

 011090 0 2 4.0 0.128 .95 .05 

 111090 0 2 4.0 0.128 .95 .05 

 011190 0 2 4.0 0.128 .95 .05 

*** Record 17 

 0 1 0 

*** Record 18 

 0 35 0.5 

*** Record 19 -- STITLE 

Loring Silt Loam; HYDG: C 

*** Record 20 

 155 0 0 0 0 0 0 0 0 0 

*** Record 26 

 0 0 0 

*** Record 33 

 6 

 1 13 1.4 0.385 0 0 0 

 0.7220280.722028 0 

 0.1 0.385 0.151 1.28 35.8 

 2 23 1.4 0.37 0 0 0 

 0.7220280.722028 0 

 1 0.37 0.146 0.49 35.8 

 3 33 1.4 0.37 0 0 0 

 0.7220280.722028 0 

 3 0.37 0.146 0.16 35.8 

 4 30 1.45 0.34 0 0 0 

 0.7220280.722028 0 

 5 0.34 0.125 0.12 35.8 

 5 23 1.49 0.335 0 0 0 

 0.7220280.722028 0 

 1 0.335 0.137 0.07 35.8 

 6 33 1.51 0.343 0 0 0 

 0.7220280.722028 0 

 3 0.343 0.147 0.06 35.8 

***Record 40 

 0 

 YEAR 10 YEAR 10 YEAR 10 1 

 1 

 1 ----- 

 7 YEAR 

 PRCP TCUM 0 0 

 RUNF TCUM 0 0 

 INFL TCUM 1 1 

 ESLS TCUM 0 0 1.0E3 

 RFLX TCUM 0 0 1.0E5 

 EFLX TCUM 0 0 1.0E5 

 RZFX TCUM 0 0 1.0E5 
14

stored as trifloxystrobin Eco Rev.out 

Chemical: trifloxystrobin 

PRZM environment: MSsoybeanSTD.txt modified Tueday, 29 May 2007 at 12:58:06 

EXAMS environment: pond298.exv modified Thuday, 29 August 2002 at 16:33:29 

Metfile: w03940.dvf modified Wedday, 3 July 2002 at 10:05:46 

Water segment concentrations (ppb) 

Year Peak 96 hr 21 Day 60 Day 90 Day Yearly 

1961 0.351 0.2401 0.1259 0.07877 0.05274 0.01301 

1962 0.3413 0.2379 0.1104 0.07319 0.04911 0.01211 

1963 0.3365 0.2291 0.1019 0.06605 0.04423 0.01091 

1964 0.3567 0.2396 0.1342 0.07717 0.05155 0.01268 

1965 0.3568 0.2399 0.1344 0.07637 0.051 0.01258 

1966 0.3613 0.2474 0.1407 0.08331 0.05575 0.01375 

1967 0.3586 0.2487 0.1372 0.08651 0.05803 0.01431 

1968 0.3464 0.2402 0.1188 0.07749 0.05202 0.01279 

1969 0.3461 0.2405 0.1183 0.07747 0.05201 0.01283 

1970 0.4071 0.3023 0.1501 0.08946 0.05992 0.01478 

1971 0.3389 0.2346 0.1062 0.07017 0.04696 0.01158 

1972 0.3427 0.2406 0.1128 0.0752 0.05046 0.01241 

1973 0.3415 0.2184 0.1109 0.06569 0.04385 0.01081 

1974 0.3526 0.2341 0.1286 0.07839 0.05246 0.01293 

1975 0.3475 0.2333 0.1244 0.07668 0.05131 0.01265 

1976 0.3651 0.2547 0.146 0.09565 0.06458 0.01588 

1977 0.3557 0.2398 0.1328 0.08 0.05349 0.0132 

1978 0.355 0.2367 0.1318 0.0753 0.05029 0.0124 

1979 0.351 0.2459 0.1258 0.0832 0.05599 0.01381 

1980 0.3583 0.2425 0.1366 0.0852 0.05717 0.01406 

1981 0.3479 0.2269 0.1211 0.07257 0.04851 0.01197 

1982 0.3495 0.2308 0.1235 0.07506 0.05019 0.01238 

1983 0.3471 0.2326 0.1199 0.07441 0.04985 0.01229 

1984 0.3359 0.2414 0.1009 0.06961 0.04661 0.01147 

1985 0.3426 0.2148 0.1127 0.06519 0.04352 0.01073 

1986 0.3499 0.2589 0.1333 0.07659 0.05116 0.01261 

1987 0.3635 0.2513 0.1437 0.08394 0.05612 0.01384 

1988 0.5296 0.3592 0.1846 0.09573 0.06392 0.01572 

1989 0.3532 0.2334 0.1292 0.07727 0.05171 0.01275 

1990 0.3538 0.2346 0.1301 0.07725 0.05164 0.01274 

Sorted results 

Prob. Peak 96 hr 21 Day 60 Day 90 Day Yearly 

0.032258064516129 0.5296 0.3592 0.1846 0.09573 0.06458 0.01588 

0.0645161290322581 0.4071 0.3023 0.1501 0.09565 0.06392 0.01572 

0.0967741935483871 0.3651 0.2589 0.146 0.08946 0.05992 0.01478 

0.129032258064516 0.3635 0.2547 0.1437 0.08651 0.05803 0.01431 

0.161290322580645 0.3613 0.2513 0.1407 0.0852 0.05717 0.01406 

0.193548387096774 0.3586 0.2487 0.1372 0.08394 0.05612 0.01384 

0.225806451612903 0.3583 0.2474 0.1366 0.08331 0.05599 0.01381 
15

0.258064516129032 0.3568 0.2459 0.1344 0.0832 0.05575 0.01375 

0.290322580645161 0.3567 0.2425 0.1342 0.08 0.05349 0.0132 

0.32258064516129 0.3557 0.2414 0.1333 0.07877 0.05274 0.01301 

0.354838709677419 0.355 0.2406 0.1328 0.07839 0.05246 0.01293 

0.387096774193548 0.3538 0.2405 0.1318 0.07749 0.05202 0.01283 

0.419354838709677 0.3532 0.2402 0.1301 0.07747 0.05201 0.01279 

0.451612903225806 0.3526 0.2401 0.1292 0.07727 0.05171 0.01275 

0.483870967741936 0.351 0.2399 0.1286 0.07725 0.05164 0.01274 

0.516129032258065 0.351 0.2398 0.1259 0.07717 0.05155 0.01268 

0.548387096774194 0.3499 0.2396 0.1258 0.07668 0.05131 0.01265 

0.580645161290323 0.3495 0.2379 0.1244 0.07659 0.05116 0.01261 

0.612903225806452 0.3479 0.2367 0.1235 0.07637 0.051 0.01258 

0.645161290322581 0.3475 0.2346 0.1211 0.0753 0.05046 0.01241 

0.67741935483871 0.3471 0.2346 0.1199 0.0752 0.05029 0.0124 

0.709677419354839 0.3464 0.2341 0.1188 0.07506 0.05019 0.01238 

0.741935483870968 0.3461 0.2334 0.1183 0.07441 0.04985 0.01229 

0.774193548387097 0.3427 0.2333 0.1128 0.07319 0.04911 0.01211 

0.806451612903226 0.3426 0.2326 0.1127 0.07257 0.04851 0.01197 

0.838709677419355 0.3415 0.2308 0.1109 0.07017 0.04696 0.01158 

0.870967741935484 0.3413 0.2291 0.1104 0.06961 0.04661 0.01147 

0.903225806451613 0.3389 0.2269 0.1062 0.06605 0.04423 0.01091 

0.935483870967742 0.3365 0.2184 0.1019 0.06569 0.04385 0.01081 

0.967741935483871 0.3359 0.2148 0.1009 0.06519 0.04352 0.01073 

0.1 0.36494 0.25848 0.14577 0.089165 0.059731 0.014733 

 Average of yearly averages: 0.012866 

Inputs generated by pe5.pl - Novemeber 2006 

Data used for this run: 

Output File: trifloxystrobin Eco Rev 

Metfile: w03940.dvf 

PRZM scenario: MSsoybeanSTD.txt 

EXAMS environment file: pond298.exv 

Chemical Name: trifloxystrobin 

Description Variable Name Value Units Comments 

Molecular weight mwt 408.4 g/mol 

Henry's Law Const. henry 2.25E-8 atm-m^3/mol 

Vapor Pressure vapr torr 

Solubility sol 61 mg/L 

Kd Kd 35.8 mg/L 

Koc Koc mg/L 

Photolysis half-life kdp 14.8 days Half-life 

Aerobic Aquatic Metabolism kbacw 1.92 days Halfife 

Anaerobic Aquatic Metabolism kbacs 2.16 days Halfife 

Aerobic Soil Metabolism asm 0.96 days Halfife 

Hydrolysis: pH 7 0 days Half-life 

Method: CAM 2 integer See PRZM manual 

Incorporation Depth: DEPI 4 cm 

Application Rate: TAPP 0.128 kg/ha 
16

Application Efficiency: APPEFF 0.95 fraction 

Spray Drift DRFT 0.05 fraction of application rate applied to pond 

Application Date Date 01-10 dd/mm or dd/mmm or dd-mm or dd-mmm 

Interval 1 interval 10 days Set to 0 or delete line for single app. 

app. rate 1 apprate kg/ha 

Interval 2 interval 21 days Set to 0 or delete line for single app. 

app. rate 2 apprate kg/ha 

Record 17: FILTRA 

 IPSCND 1 

 UPTKF 

Record 18: PLVKRT 

 PLDKRT 35 

 FEXTRC 0.5 

Flag for Index Res. Run IR EPA Pond 

Flag for runoff calc. RUNOFF none none, monthly or total(average of entire run) 
17

Attachment 4 

Aquatic RQs 

Effect Endpoint (ug/L) EEC (ug/L) RQ 

Acute Freshwater Fish 96-hr LC50:14a Peak: 0.36 0.026 

Chronic Freshwater 

Fish 

NOAEC: 4.3b 60-day: 0.09 0.021 

Acute Freshwater 

Invertebrates 

48-hr EC50: 25c Peak: 0.36 0.014 

Chronic Freshwater 

Invertebrates 

NOAEC: 2.8d 21-day: 0.15 0.054 

Acute 

Marine/Estuarine Fish 

LC50: 78e Peak: 0.36 0.005 

Acute 

Marine/Estuarine 

Invertebrates 

96-hr LC50: 8.6f Peak: 0.36 0.042 

Chronic 

Marine/Estuarine 

Invertebrates 

NOAEC: 0.96g 21-day: 0.15 0.156 

Non-vascular aquatic 

plants 

96-hr EC50: 37h 

NOAEC: 10 

Peak: 0.36 

Peak: 0.36 

0.010 

0.036 a

 96-hr LC50 in rainbow trout (MRID 44502803) b

 Early life stage toxicity study in rainbow trout (MRID 44502804) 

c

 48-hr EC50 in Daphnia magna (MRID 44496611) d

 21-day Daphnia magna study (MRID 44496615) 

e

 LC50 in Sheepshead minnow (MRID 44496612) f

 96-hr LC50 in mysid shrimp (MRID 44496614) g

 The estuarine/marine chronic NOAEC was estimated using acute to chronic ratios (ACRs) derived from daphnid 

acute (EC50=25 ppb) and chronic (NOAEC = 2.8 ppb) toxicity values. The ACR was 8.9. Therefore, the estimated 

NOAEC in mysid shrimp would be 8.6/8.9 = 0.96 ppb. 

h 96-hr EC50 in algae (MRID 44496724); the EC50 and NOAEC in duckweed were considerably higher (MRID 

44496725). Therefore, risk to vascular plants is presumably lower than risk to non-vascular plants such as algae.
18

Attachment 5 

Terrestrial RQs 

Upper Bound Kenaga, Acute Avian Dose-Based Risk Quotients 

EECs and RQs 

Short Grass Tall Grass 

Broadleaf 

Plants/ 

Small Insects 

Fruits/Pods/ 

Seeds/ 

Large Insects 

Size Class Granivore 

(grams) 

Adjusted 

LD50 

EEC RQ EEC RQ EEC RQ EEC RQ EEC RQ 

20 1038.45 77.69 0.07 35.61 0.03 43.70 0.04 4.86 0.00 1.08 0.00 

100 1322.00 44.30 0.03 20.31 0.02 24.92 0.02 2.77 0.00 0.62 0.00 

1000 1867.37 19.84 0.01 9.09 0.00 11.16 0.01 1.24 0.00 0.28 0.00 

Upper Bound Kenaga, Subacute Avian Dietary Based Risk Quotients 

EECs and RQs 

Short Grass Tall Grass 

Broadleaf 

Plants/ 

Small Insects 

Fruits/Pods/ 

Seeds/ 

Large Insects 

LC50 EEC RQ EEC RQ EEC RQ EEC RQ 

5050 68.22 0.01 31.27 0.01 38.37 0.01 4.26 0.00 

Size class not used for dietary risk quotients 

Upper Bound Kenaga, Chronic Avian Dietary Based Risk Quotients 

EECs and RQs 

Short Grass Tall Grass 

Broadleaf 

Plants/ 

Small Insects 

Fruits/Pods/

Seeds/ 

Large 

Insects 

NOAEC 

(ppm) EEC RQ EEC RQ EEC RQ EEC RQ 

320 68.22 0.21 31.27 0.10 38.37 0.12 4.26 0.01 

Size class not used for dietary risk quotients 
19

Upper Bound Kenaga, Acute Mammalian Dose-Based Risk Quotients 

EECs and RQs 

Short Grass Tall Grass 

Broadleaf 

Plants/ 

Small Insects 

Fruits/Pods/ 

Seeds/ 

Large Insects 

Granivore 

Size Class 

(grams) 

Adjusted 

LD50 

EEC RQ EEC RQ EEC RQ EEC RQ EEC RQ 

15 10989.15 65.04 0.01 29.81 0.00 36.58 0.00 4.06 0.00 0.90 0.00 

35 8891.40 44.95 0.01 20.60 0.00 25.28 0.00 2.81 0.00 0.62 0.00 

1000 3845.80 10.42 0.00 4.78 0.00 5.86 0.00 0.65 0.00 0.14 0.00 

Upper Bound Kenaga, Chronic Mammalian Dietary Based Risk Quotients 

EECs and RQs 

Short Grass Tall Grass Broadleaf Plants/ 

Small Insects 

Fruits/Pods/ 

Seeds/ 

Large Insects 

NOAEC (ppm) 

EEC RQ EEC RQ EEC RQ EEC RQ 

1500 68.22 0.05 31.27 0.02 38.37 0.03 4.26 0.00 

Size class not used for dietary risk quotients 

Upper Bound Kenaga, Chronic Mammalian Dose-Based Risk Quotients 

EECs and RQs 

Short Grass Tall Grass 

Broadleaf 

Plants/ 

Small Insects 

Fruits/Pods/ 

Seeds/ 

Large Insects 

Granivore Size Class 

(grams) 

Adjusted 

NOAEL 

EEC RQ EEC RQ EEC RQ EEC RQ EEC RQ 

15 164.84 65.04 0.39 29.81 0.18 36.58 0.22 4.06 0.02 0.90 0.01 

35 133.37 44.95 0.34 20.60 0.15 25.28 0.19 2.81 0.02 0.62 0.00 

1000 57.69 10.42 0.18 4.78 0.08 5.86 0.10 0.65 0.01 0.14 0.00