vl

LAKE NAME: BANKHEAD LAKE CONTINUED
STORET NUMBER: O10L

06 08 73 08 25 73 10 30 73

{ ALGAL ALGAL { ALGAL sf

1 UNITS =f UNITS =f UNITS |
TAXA FORM {S %C PER ML {S %C PER ML [S$ %C PER ML |
FLAGELLATES CEL «(f211999f 281 «fLi4lelf «6476 «OE I {
GYRCSIGMA CBTUSATUM cEL tt 1 x tt { x tt { x |
KIRCENERIELLA cer of I 208] 40 U1 1 tt { 1
LUNATE CELL cer oft ! imi { i [0.91 9 f
LYNGBYA LIMNETICA FIle Lt i} {1 28! 32) 1 ft 106! 15 4
MELOSIRA #2 ceu ot 1 151 2.01 23 «td { {
MELOSIRA DISTANS CEL 141 74! «105141 4.81 55 15] 6.31 6. f
MERI SMOPEDIA cor ft ( x tt { tf { {
MERISMOPEDIA MARSSONIT cou oft { tt ! x tf i '
MICROCYSTIS INCERTA ? cor ot I | 11 0.71 8 tt | !
NAVICULA cer ott | 1 1.4 we Id ! |
NITZSCHIA cee ott t tt { {1 { x of
NITZSCHIA #1 ceu ot | ! i ' x tt ' !
NITZSCHIA #2 cet ft t tt 1 x tt { 1
CPHIOCYTIUM #2 cor ot I I tt 1 {1 0.91 9 f
CSCILLATCRIA Fr oO 1 x tt { im { i
OSCILLATORIA ANGUSTA FIL] oJ | 2.81 40 [211003f 19 1 t { {
PANDCRINA MORUM cor oft ! im! 1 x [1 o.9t 9 4
PEDIASTRUM BIRACIATUM tt { tt ' tt ‘ i
Ve LONGECGRNUTUM 2? co ft | tl { tt { x |
PEDTASTRUM TETRAS tt | tt | 1 \
Ve TETRAODON cor ft 1 x Tt 1 tt {
PEANATE CIATOM cer ott ! 1 | x tt | !
PENNATE OLATOM #1 cee I { x tt ( (1 o.9f 9 1
PENNATE CIATOM #2 cet sot 1 x td ! {ft 1.61 is
PENNATE DIATOM #3 ceu ot I o2e3t 32, I On7t 6 tt | !
PENNATE DIATOM #4 cer ott ! {1 2.81 32. («dt I | {
PHACUS ce otf t tt { x 11 0.91 9 1
RAPHIDIOPSIS i. en { 1 | 6s8t 7m tt | 1
RHIZOSOLENIA CEL | { | | x tt 1 1
SCENEDESMUS co Ot I { 10.71 s tl | '
SCENEDESMUS #1 coo ott I tt t UT tof |


Ge

LAKE NAME: MITCHELL LAKE
STORET NUMBER: 0108

TAXA

ACHNANTHES LANCECLATA
Ve DUBIA
ACTINASTRUM HANTZSCHIT
ANABAENA 7

ANABAENA #1

ANABAENA #2

ANABAENA #3

CENTRIC DIATOM
CHLAMYOCMONAS
CHLOROGONI UM
CHLOROPHYTAN COLONY
CHLOROPHYTA4N FILAMENT
CHLOROPHYTAN LUNATE CELL
CHROOCCCCUS
CLCSTERIUM #2
CLOSTERIUM #3
COELASTRUM
CRUCIGENIA

CRUC IGENIA TETRAPEDIA
CRYPTCMCNAS
CYCLOTELLA
CYCLOTELLA MENEGHINIANA
CYMBELLA
DACTYLOCCCCOPSIS
DICTYOSPEAERIUM
OINCFLAGELLATE
DINOFLAGELLATE #1
DINOFLAGELLATE #2
EUASTRUM

EUASTRUM DENTICULATUM
EUGLENA

FLAGELLATE #1

CONTINUED
06 06 73 08 29 73 10 31 73

{ ALGAL sf ALGAL ot AccaAL Ot

{ UNITS = { UNITS = [ UNITS |
FORM {[S %€ PER ML [S %C PER ML f ac perp me

tt 1 tt { tt 1 {
cet ofl 1 tt i} x tt ! !
cou ott { tt { 1 1 0.41 12 6
Fr of ft O68 29 614 { tt 1 1
FIL oft { (2113-8{ 661 Jf ' {
FIL tt { tt ( x td ' 1
FIL of J { tt | x td ! !
cet of ft 1.7f sa ott 1 tt { t
cel ot ft t x tt | { 1 0.9! |
cet off t 11 i} x td 1 i
co. ft t it { tt { x 4
cee tt ( tt 1 f4f 729! 214 I
cet ott ! tt { tt 1 x 4
coL | ( x ff { 1 1 0.4] 12 1
cer ott t x tt { tt | 1
cet ott ! x td t tt 1 ]
cor tf ! x [1 0.51 23 «It ! !
cor J i] tt { (1 0.41 wz 4
cer td I x TF 241 114 TH { x ot
cet «oI ft (17 sett { 15] 7-0] 189 |
cer ott ! 131 6.74 319 «tt 1 1
cer ott \ x ott 1 x 16 7.0f 189 |
ce oft t 11 ' im ' x J
cee of | 5e0f) 6174 «6 CE 403d 205 t !
cor tf { 1 1.01 46 It ! !
cee ot ! tt ' tl i x 4
cEL { f 5.0] 1%4 11 | x tf i} 1
cEL 11 0.8) 29 614 ' x Fd ! !
cet ft 1 tt { x tf 1 {
CEL tt { tt i tt | x of
CEL {1 { 1 1 1.01 4o If 1 x 4
ceu of 1 6e7t 6232) | ti { i


Le

LAKE NAME: MARTIN LAKE CONTINUED
STCRET NUMBER: 0107

03 18 73 06 16 73 08 25 73 iL o1 73
' ALGAL ALGAL I ALGAL I ALGAL
' UNITS =| UNITS { UNITS UNITS

TAXA FORM {S %C PER ML IS %C PER ML [S %C PER ML IS %C PER ML

ANABAENA . Fl ott i] x od 1 iva 1 ut f ]
ANABAENA #1 Fre i Hif4s.of 456 [1123.1] 1208 | 1 Seo! 113 1
ANABAENA #2 Fi ott 1 tl e otf ! tt 1 x of
ANABAENA 43 Fie 1 i 4 {t 1 tt 1 x ot
ARKI STRCOESMUS cel tt ' it ' im 1 (f4ol 1130
ANKISTRCDESMUS 2 ceL | 413.51 310 t tt { it t t
ASTERIONELLA FORMOSA cet tat | x tt ' tt ! tt 1 {
ATTHEYA cee ott ' tt if tt { 1 f 2.01 2 ft
CHLAMYDCMONAS cee ot ! 1 ft O6l 6 tt 1 11 Leot 2a
CLCSTERIUM cee otf 1 tt 1 tt { im i xd
CCELASTRUM cou oll ' tt { it { x 1 {
CCSMAR TUM cer ott i tf 1.21 12 1 1 308! 197 TI ! I
CRUCIGENTA TETRAPEDIA co lt ! x out ' tt { 1 1 2.01 |
CRYFTOMCNAS CEL «6131 Self ol? OE ! tt 1 4 1 i
CRYPTOMONAS #1 cee ot i 4 it 1 tt ' {120.21 586)
CYCLOTELLA cel ff tell 2 tI ' tt i x 1 1 301 35 |
CYCLOTELLA STELLIGERA cer ot I 1 14 5.6! saa 1 ti i 1
CYMBELLA cee i ! tt ' x oad 1 it 1 !
CACTYLCCCCCCPSIS ca ott i 1 | Oo6t eo ! tt 1 t
DACTYLOCCCCNPSIS #1 cee ot I 1 tt { tt ' 7% t 400f 113 |
CACTYLCCCCCOPSIS #2 cer ott 1 14 ( tt 1 985 1 1 304 85 of
DINCBRYCK cet | | leat 42 td ! tt 1 26 td 1 x of
DINOFLAGELLATE #1 CEL IS 303! we tt 1 tt 1 49 tt 1 x 4
CINCFLAGELLATE #2 cet It ' tt 1 i 1 25° 131 400! 113
DINCFLAGELLATE #3 cer ott ' tt { tt | 26 6 1 {
EUGLENA cee ott 1 11 1 tt i tt 1 x 4
FILAMENT Fil ott t W4ttaett iis ot I 1 tt 1 if
FLAGELLATE cet It ' tt 1 {t 3e3f 173 TI { {
FLAGELLATE #1 cee ot | ! tt 1 ti i} (5f s.lf 1700 |
FLAGELLATES. CEL ({1{5ée7! 1300 13/1491 154 | I i 14] Bell 227 |
FRANCE IA cet ff { tt 1 ( 1 0651 25 I 1 '
FRANCETA CRCESCFERY cet ott 1 tt 4 it ' tt 1 x 4


Ov

LAKE NAME: PICKWICK LAKE CONTINUED
STORET NUMBER: 0105

06 09 73 08 15 73 10 22 73

' ALGAL ALGAL ALGAL

1 UNITS =| UNITS | UNITS

TAXA FORM [S %C PER ML [|S 2C PER ML [|S %C PER ML
PANDORINA MORUM cor tt { x tt ! tt { '
PEDIASTRUM BIRADIATUM tt ' tt i tl 1 |
Ve ? cor lt { 11 1 tet { x 4
PEODIASTRUM SIMPLEX tt ! ti | i 1 1
Ve CUODENARIUM cor otf t tt 1 xX 131 0051 12 «4
PENNATE DIATOM cet otf { im { x 1 1 '
SCENEDESMUS co off 1 1 1 3.6f 39 «64 ! |
SCENEDESMUS BREVISPINA coe 1 | | It ! x 4
SCENEDESMUS QUADRICAUDA cor ott { x tt ! x tt ' {
SCHRCEDERIA cee st I 1 x tf { x ft ! {
SYNEORA #1 cer ot I 1 {1 2041 26 6 { {
SYNEDRA #2 cee ott 1 x Ud ! x tt I {
SYNEDRA RUMPENS tt { tt ! tt | !
Ve? cee ott 1 ft i | { x 1
TETRAECRON #1 cet ott 1 (1 3.61 39 «tt { |

TCTAL 1299 1098 2596


from the subsample, as being equal to the sample value. Even though
considerable effort was made to find and identify all taxa, the Survey
samples undoubtedly contain a fair number of rare phytoplankton taxa
which were not encountered.

In the Shannon-Wiener formula, an increase in the number of taxa
and/or an increase in the evenness of the distribution of individuals
among taxa will increase the average diversity per individual from its
minimal value of zero. Sager and Hasler (1969) found that the
richness of taxa was of minor importance in determination of average
diversity per individual for phytoplankton and they concluded that
phytoplankton taxa in excess of the 10 to 15 most abundant ones have
little effect on H, which was verified by our own calculations. Our
counts are in number per milliliter and since logarithms to the base
2 were used in our calculations, H is expressed in units of bits per
individual. When individuals of a taxon were so rare that they were
not counted, a value of 1/130 per milliliter or 0.008 per milliliter
was used in the calculations since at least one individual of the
taxon must have been present in the collection.

A Survey sample for a given lake represents a composite of all
phytoplankton collected at different sampling sites on a lake during a
given sampling period. Since the number of samples (M) making up a
composite is a function of both the complexity of the lake sampled and
its size, it should affect the richness-of-taxa component of the
diversity of our phytoplankton collections. The maximum diversity
(MaxH) (i.e., when the individuals are distributed among the taxa as
evenly as possible) was estimated from log, S, the total diversity (D)
was calculated from HN, and the evenness component of diversity (J)
was estimated from H/MaxH (Pielou 1966). Also given in the Appendix
are L (the mean number of individuals per taxa per milliliter) and K
(the number of individuals per milliliter of the most abundant taxon
in the sample).

Zand (1976) suggests that diversity indices be expressed in units
of "sits", i.e., in logarithms to base S (where S is the total number
of taxa in the sample) instead of in "bits", i.e., in logarithms to
base 2. Zand points out that the diversity index in sits per
individual is a normalized number ranging from 1 for the most evenly
distributed samples to 0 for the least evenly distributed samples.
Also, it can be used to compare different samples, independent of the
number of taxa in each. The diversity in bits per individual should
not be used in direct comparisons involving various samples which
have different numbers of species. Since MaxH equals log S, the ex-
pression in sits is equal to log. S, or 1. Therefore diversity in sits
per individual is numerically eqhivalent to J, the evenness component
for the Shannon-Wiener formula.


0s

LAKE NAME: LAKE PURDY CONT TINUED
STORET NUMBER: O115

08 30 73 ll 02 73

ALGAL ALGAL

! |
{ UNITS | UNITS

TAXA FORM JS %C PER ML [S &C PER ML

ANABAENA #1 ) FIL [17.9 321) {4 6.21 «318
ANABAENA #2 FIL of f 164! st Id l {
ANABAENA #3 FIL td ' X Tb oe2b 212 4
ANABAENA #4 FL of { {4 2.8f 141 1
ANKISTRCDESMUS FALCATUS cer so I ! 1 O.4t 1s
ARTHROSPIRA CEL {ft O09 38 6I't { !
BCTRYOCCCCUS BRAUNTY cou of | 0.5) 19 ff 0041 1s |
CARTERIA cer 4 ' tt ! x of
CENTRIC DIATOM cer 14 1 11 ! x ot
CENTRIC DIATOM #2 cel ott { x 1 ( I
CERATIUM HIRUNDINELLA cet | 1 0.91 38 (dE AL ‘ '
CHLAKY DCMONAS cer fl { tt ' x 1
CHRCCCCCCUS co. otf ' x tt ' '
CHRYSOCOCCUS col of I O69 38 (Cd I 1 '
CLCSTERIUM cee Ot I 1 x tl { |
CLCSTERIUM #1 cer ott l tf o.4t we J
CLOSTERIUM #2 cer ott t 1 ft 0.41 we of
COELASTRUM MICRCPORUF con of | ' It o.7! 35 J
COELOSPHAERIUM NAEGELI ANUM COL | ft 4e4t «6180 FL 1402! 724
CRUCIGENTA APICULATA co otf ‘ x tt | x |
CRYPTOMONAS cel ott | {$3081 194 |
CYCLOTELLA cer of I 1.91 we It 1 !
CYCLOTELLA STELLIGERA cee ott ' {1 1.71 es |
CYMBELLA TURGIDA cee of A 1 it J x |
OACTYLOCCCCOPSIS ceL otf 14d s7 It ' !
DINOFLACELLATE . ceL 1 | O65! 19 tf teal nm ot
EUOCRINA ELEGANS cor tl ! xX 1 1 0.4) 1
EUGLENA cer off | tt 1 x ot
EUGLENA CXYURIS ? cele oI 4 4 x a4 { {
FLAGELLATE #1 cer ot | | 13/1201) 618 «|
FLAGELLATES CEL «141 9e3f «60377 «15 5621 265
GYRCSIGMA ce 14 I 11 | x |


LAKE NAME: WILSON LAKE CONTINUED
STCRET NUMBER: O114

06 09 73 08 16 73 lo 22 73

' ALGAL ALGAL | ALGAL

| UNITS [ UNITS I UNITS
TAXA FORM 4s ZC «PER ML OIS tC PER ML IS 3C =PER ML
SYNEDRA #2 ceL ott { (ft 15f 126 14 { f]
TETRAECRCN cee ! {toast 31 Ct { 1
TETRAEDRCN MINIMUM ial ( it i} i t 1
Ve SCROBICULATUM cee ot ' im ! x Vd | i
TETRAECRCN MUTICUM ceu tJ ' tt { x at 1 {
TREUBARTIA cee ot ! 11is5t 126 +4 i i

TOTAL 3352 8596 1652


9b

LAKE NAMES WILSCN LAKE CONTINUED
STORET NUMBER: 0114

06 09 73 08 16 73 10 22 73
' ALGAL ALGAL | ALGAL
{ UNITS | UNITS =| UNITS

TAXA FORM |S %C PER ML 1S C PER ML [S %C PER ML

ANABAENA ? FIL tf 1 tt x tl { |
ANKISTRODESMUS 2? cen ot I { i] t x Tt { !
ANKISTRCDESMUS FALCATUS CEL {1 0.7) 24 tt 1 tt ! t
CARTERIA cet otf t tt { x Ud | |
CENTRIC OTATOM #1 cer ot ft O.7] a td { tt ' x 4
CHLAMYDCMCNAS ceL dd 1 it ! to ..e2t 19 Of
CELOROPHYTAN LUNATE CELL CEL tt { { | 0.7] 63 tf i t
CFROOCOCCUS cor tt 1 {12.24 189 Ef 1 {
COCCONEIS PLACENTULA 1f 1 im 1 tt 1 1
Ve EUGLYPTA cer ott ! x tt { ti ! {
CRUCIGENTA co tt { tt { x tl ! {
CRYPTOMCNAS cer ott i] tt | x tl y {
CRYFTOMONAS #1 CEL «131 4e2t 0 142d ' 13115031 2530 9
CRYPTOMCNAS #2 cel Ot ] x ot ( im 1 x ot
CYCLGTELLA #1 cer of I 1.4] 47 14 { im ' '
CYCLOTELLA #2 CEL «141 4e21 142) oT 1 165d) 126 «Edt 82h 13e It
CYCLOTELLA #4 ceo off ' 14] oof 567 Ff 1 i
CYCLOTELLA MENEGHINI ANA cee ss I i] [1 5! 126 It { 1
CACTYL OCOCCOPS IS cer ott ! tt | {1 2.41 39 )C«#d
DICHCTCMCCOCCUS cor oft ! 1 { x 1 { '
DINOFLAGELLATE cet | { x tt | tt { {
DINOFLAGELLATE #1 cee Ot I { {1 0.71 63 (14) 4.71 78 O44
CINCFLAGELLATE #2 ceu ot I | im | x 1 | !
EUGLENA cer oT 1 4 ' it 1 x |
FLAGELLATE #1 CEL «Sf 8.41 283 «1 I 7.01 «598 =«(I2124e8f «6409
FLAGELLATE #2 ter ff 1 x Vt t i { \
FLAGELLATES CEL «([2t14e1l 472 «= (3)41641 976 ts 3 e5t se |
FRANCETA . ceu Ot I im { x it | 1
GOLENKINIA cer ot I | tt ! x Ut { \
LAGERHEIMIA CILTATA cer ott { tt | x tl ! {
LYNGBYA LIMNETICA Foot t i tt { x Ut | {
MELOSIRA #2 cet EI ' x 14 0.71 63 151 2041 39d


sl

LAKE NAME: GANTT RES. CONTINUED
STCRET NUMBER: 0103

06 19 73 os 30 73 11 03 73
' ALGAL ot ALGAL I ALGAL
{ UNITS =f UNITS I UNITS

TAXA FORM {S&C PER ML IS %C PER ML [S %C PER ML

OSCILL ATORIA FIL) {41 431 17 TT I tt ' 1
GSCILLATORIA LIMNETICA Flt i 14113071 32 «151 5e4t 37
PANDORINA MORUM cor tt { ti { tt { x 4
PENNATE CIATOM #1 CEL 15] 6.34 25 It ! tt | x 1
PENNATE DIATOM #2 cel Ot i x of { 1 1 {
PENNATE DIATOM #3 cer ot ft 1 (1 3.41 s tt { '
FENNATE OTATOM #4 cee ott { {1 { x tt { x 1
PHACUS PYRUM cer ot ! 11l 3.4! aout { {
RHIZOSOL ENTA cet | { 2.08 6 tl ! tt | x J
SCENEDESMUS, cou ff { x wt { im { !
SCENEDESMUS #1 cor ott { tt ! tt ' x |
SCENEDESMUS #2 cor ot ft ' ti | tt ( x 1
STAURASTRUM cet Ot i x 1 f 304! Cn | ! 1
SYNEDRA ? cer tt { tt | tt { x of
SYNEORA #1 cet tt { tt | x tt 1 {
SYNEDRA #2 cee ott I {1 3041 aout 1 {
TETRAEDRON ceu tt | x td f ia I x ot

TOTAL 394 233 680


9€

LAKE NAME: MITCHELL LAKE CONTINUED
STORET NUMBER: 0108

06 06 73 08 29 73 10 31 73
{ ALGAL ALGAL ALGAL
| UNITS f UNITS UNITS

TAXA FORM {S %C PER ML IS  &C PER ML 2C PER ML

FLAGELLATES cet 5] {saa tt i
LUNATE CELL cet ott { 58 tt {
LYNG BYA | 1 tt !
LYNGBYA CIRCUMCRETA FIL ofl i 1 1 0.5 23 |
LYNGBYA LIMNETICA FIL OE { 111.9 91 1
MELOSIRA #2 CEL f1fl5e0f 521 | | 209 137 |
MELOSIRA #3 CEL [4110081 376 «1 I !
MELOSIRA #4 CEL ft 4e2f 145 TE 403 205 1
MELGSIRA #5 CEL 13) 568 203 «| I |
MELOSIRA DISTANS CEL «(12122651 97821130051 1459 {
MERISMOPEDIA MARSSONIT cor tt ! {1 1.9 31 |
MERTSMCPEDIA MINIMA ceu ott i tt !
MICROCYSTIS cor ff ! i x !
NAVICULA cert ! tt {
NAVICULA GASTRUM cee st ' 1 !
NITZSCHIA #1 cee tt 1 1 1 3.8 182 {
NITZSCHIA #2 cee ot t 1 1.5 a {
OSCILLATORIA Fil ott ' x td '
CSCILLATORIA #1 Fit { 1 {0.5 23 !
PARDGRINA MORUM cou tt ! x tt x (
PEDTASTRUM BIRACIATUM tt 1 tt !
Ve. LCNEECORNUTUN cor ott ! tt x t
PEDIASTRUM DUPLEX 1 | 14 |
Ve RETICULATUM cor ott { x tf 1
PECIASTRUM SIMPLEX co tt { tt x {
PENNATE DIATOM cet ot ! x ff x 1
PENNATE DIATOM #2 cet of I 0.8t 29 It x {
PENNATE DIATOM #3 CEL oI CT 008f 29° 1 | O65 23 i
PENNATE DIATOM #4 cer ott { x tt !
PHACUS cer ott I tt {
SCENEDESMUS. cor tt | x tf 1
SCENEDESMUS #1 cou Ot I ! {1 1.0 46 1



FOREWORD

Protection of the environment requires effective regulatory
actions which are based on sound technical and scientific information.
This information must include the quantitative description and linking
of pollutant sources, transport mechanisms, interactions, and re-
sulting effects on man and his environment. Because of the complexities
involved, assessment of specific pollutants in the environment requires
a total systems approach which transcends the media of air, water, and
land. The Environmental Monitoring and Support Laboratory-Las Vegas
contributes to the formation and enhancement of a sound integrated moni-
toring data base through multidisciplinary, multimedia programs designed
to:

* develop and optimize systems and strategies for moni-
toring pollutants and their impact on the environment

* demonstrate new monitoring systems and technologies
by applying them to fulfill special monitoring needs
of the Agency's operating programs

This report presents the species and abundance of phytoplankton
in the 11 lakes sampled by the National Eutrophication Survey in the
State of Alabama, along with results from the calculation of several
commonly used biological indices of water quality and community structure.
These data can be used to biologically characterize the study lakes,
and as baseline data for future investigations. This report was written
for use by Federal, State, and local governmental agencies concerned with
water quality analysis, monitoring, and/or regulation. Private industry
and individuals similarly involved with the biological aspects of water
quality will find the document useful. For further information contact
the Water and Land Quality Branch, Monitoring Operations Division.

Ag b Mego

Director
Environmental Monitoring and Support Laboratory
Las Vegas


62

LAKE NAME: LAY LAKE CONTINUED
STORET NUMBER: 0106

06 06 73 08 29 73 10 31 73
{ ALGAL ALGAL ALGAL
! UNITS =f UNITS = I UNITS

TAXA FORM {S %C PER ML IS %C PER ML [S %C PER ML

PEDIASTRUM DUPLEX tt 1 11 1 TT 1 1
Ve 2 cor ott 1 x tt i} tt i} {
PECTASTRUM DUPLEX t{ { tt { im! 1 |
Ve RETICULATUM cou tt 1 { { 0031 42 tt 1 {
PECIASTRUM SIMPLEX cou otf { tt { x tt { {
PEDIASTRUM TETRAS ti { 1 I 11 { 1
Ve TETRAODON cor ot ! tt i} tt ! x ot
PENNATE OI ATOM cet of f O67 oz ott 1 tt t !
PEKNATE CIATOM $1 ce tt ! t t [lost 20 1
PENNATE DIATOM #2 cee ot I { 11 oo7! na { {
PENNATE CIATOM #3 cet tl iy tt 1 x Ut { {
PENNATE DIATOM #5 cer otf 1 tt { x tt { x ot
PENNATE DIATOM #6 cer otf { tt 1 14 { x ot
SCENEDESMUS cor { [ 0.31 ES ee | ( x ft { t
SCENEDESMUS ABUNDANS co. tt i 1 1 0.31 42 {1 171 6. 1
SCENEDESMUS ACUMINATUS cet 1 1 O63 31 6«dit ' x tt { x ft
SCENEDESMUS ARCUATUS cou =f | 0031 3h ot dd { ti { '
SCENEDE SMUS BICAUDATUS co tt 1 1 { x ft { {
SCENEDESMUS BIJUCA cor oft { 1 10.34 42 tt { '
SCENEDESMUS ECORNIS 14 { 't { tt { '
Ve DISCIFORMIS cor | f o.7] oz tt i tt { (
SCENEDESMUS GRANULATUS 14 i it { tl | l
Fe DISCIFCRMIS coL {1 | tt 1 tt 1 x |
SCENEDESMUS QUADRICAUDA cou off Lot 93 Tf 2e6f 337 {ff 208f 102 |
SCHROEDERTA cel ot ! 1 1 0.31 42 it ! {
SCHROEDERIA SETIGERA ceL of I OT! oz It ! x tt ! '
STEPHANODI SCUS CEL oT ft le7l 155 1 140! 506 11 56! 202 |
SYNEORA CEL (1 0031 31 6d | x tt | {
TETRAEDRON MINIMUM CEL If 0031 31 1 ft 0.31 42 11 t {
TETRASTRUM coL 1d 1 x tt 1 x Ud ! {
TREUBARIA cee Ot 1 (1 o.3t 42 44 t 1

TOTAL 9355 12735 3577


SL

LAKE NAME: BANKHEAD LAKE CONT INUED
STORET NUMBER? O1OL

06 08 73 08 25 73 10 30 73

{ ALGAE Of ALGAL | ALGAL 1

1 UNITS = { UNITS = [| UNITS = |
TAXA Form {S$ %C PER ML IS %C PER ML [S %C PER ML I
SCENEDESPUS DENT ICULATUS coo TE { (ft 2.81 32. TT 094 9
SCENEDESMUS GPOLIENSIS cou of fo lelt we tt ! x 1 { '
SCENEDESMUS QUADRICAUDA cor ott { im | {1 5.5t 53 of
STAURASTRUM cet of f O06! 8 tt 1 1 { x of
STAURASTRUM #1 cet ott ! x Tt { ti { t
STAURONEIS ANCEPS it ' Vt \ Vi ' |
Ve GRACILIS cer ott | 1a 1 x td ! !
SYNEDRA ceL oI ft 203! 32 BfL1Le7f 1350 dE 204d 234
TABELLARIA ceu ot t x ot { | 1 0.91 a 4
TETRAECRCN TRIGONUM tt ( (1 { tt ! !
Ve GRACILE cer sot I ' tt { x tft ! 1
TETRASTRUM HETERACANTHUM cor of ' tt ! {1 1 x ot
TRACHELCMONAS ? #2 ceL of ft O66] 8 ott { {1 1 '
TRACHELOMONAS #1 cer ff Oe! 8 tf { i { ‘

TOTAL 1413 1157 964


le

LAKE NAME: GUNTERSVILLE RES. CCNTINUED
STCRET NUMBER: 0106

06 08 73 08 16 73 10 22 73
! ALGAL I ALGAL ft ALGAL
t UNITS { UNITS I UNITS

TAXA FORM |S %C PER ML {[S %C PER ML [S %C PER ML

EUGLENA ce TT tr x TT 1a 3537 1
EUGLENA #1 ceL of ft O71 206 It ' im { !
EUGLENA #2 cee ot I ! x tf { tt ! {
FLAGELLATE ceL ott ( imi | {2129.51 9029 |
FLAGELLATE #1 ceL of { 4ef 61790 «ft t tt | {
FLAGELLATES CEL Of UC Gell 6154 15] Se4f sto Et { t
GOLENK INTA ceL ot | O74 26 $414) 135 II { x 1
GYRCSIGMA SPENCERIT cel otf { ti { 11 { x |
KIRCHNERTELLA LUNARIS ti | 1 ! tt { !
Ve IRREGULARIS cer tf ! im 1 it { x 4
LYNGBYA Fie td t x tt i {1 ! !
LYNGBYA #1 FIL ott I 1 f 0.51 45 1 i !
LYNGBYA LIMNETICA Fir ot 1 {18.91 855 It 2-11 645 1
WELOSIRA #2 CEL «11123271 «52231 47!) «45014 3e2t 968
MELOSIRA #4 CEL 14/2206] 945 | I ! x 4 { x |
MELOSIRA DISTANS CEL [5/1921 717) | 303t) 315) Tf est) 6537 OE
MERISMOPEDIA MINIMA cor tI ' im { {1 { O.4f 108 1
MERISMOPEDIA PUNCTATA cor ott 1 11 0.51 45 14 I '
MERISMOPEDIA TENUISS IMA co ott { imi | x tt { {
MERISMOPEDIA TROLLERI cor ott | tt ! im 1 x |
MICROCYSTIS co tf ! (41 3.31 315 of 1 1 i]
MICROCYSTIS INCERTA cor ott | 1 { tt i x |
NAVICULA #1 cet ott ' x tt { {1 ! !
NAVICULA CAPITATA ce ott ! x tt | tt i x 1
NAVICULA MENISCULUS tt 1 tt 1 1 ' !
Ve UPSALTENSIS cEL | i] tt { 11 1 x 1
NITZSCHIA #1 ce ot ! 11303! 315 ft 1 | i
NITZSCHIA #2 ceu ot} 1 | 1 x tf { '
NITZSCHIA 43 cer ot | ! | 1 {5111.21 3640 |
QOCYSTIS SUBMARINA cer ot I ! x tt | tt 1 1
OSCILLATORIA FIL tt 1 x tt { ti { !
CSCILLATORIA LIMNET ICA Fl otf 1 (1.91 160 [1 3.9f 1182 |


ral

LAKE NAME: GANTT RES. CONTINUED
STCRET NUMBER: 0103

06 19 73 08 30 73 11 03 73

‘ ALGAL oI ALGAL I atcaL of

' UNITS | UNITS [| UNITS J
TAXA FORM | ZC PER ML IS %C PER ML IS %C PER ML TI
ANABAENA Fit 1 x tf 1 x tf i !
ANK ISTROODESMUS cee of I 4.31 1? { tt | {
CENTRIC DIATOM #1 cel of I 43! wz 1 131 6.8] 46 1
CENTRIC DIATOM #2 ceL of 2.01 8 11 609! leo otf ! {
CHLAMY DCMCKAS, cet of] { 1 { 3.4f 8 ff { {
CHROCCCCCUS cor of | ! x td | tt ! 1
CLCSTERIUM cet oft 1 x tt { {1 i x 4
CCSMAR TUM cet of { 2.01 8 tt { tt 103! 9 ot
CRUCIGENIA TETRAPEDIA cou | { 2.0t 8 tf | tt 1 {
CYMBELLA cee ott { x tt 1 {1 i 1
LACT YLOCCCCOPSIS ceL ot ft 2.01 8 1 120031 24 {2[l6e2! 110 |
DINOBRYON cer ott 1 tt ! 1 1 1.3! 9 4
CINOFLAGELLATE #1 cet of | 2.01 8 {2h 3.41 8 ff 1.3f 9 f
DINCFLAGELLATE #2 CEL 12112671 so ON ! i i 1
DINOFLAGELLATE #3 cee ot I ] tt ! 1 T2061 1s
EUGLENA #1 cet off { x 1 3641 8 f 1 t.31 9 1
EUGLENA #2 cer ot I 1 x tt 1 tt i !
EUGLENA #3 cet ot { tt t x tt { '
FLAGELLATE CEL L1143e1f «6170 EL { tt ! i
FLAGELLATE #1 ceu ott ! 1 1 3.41 8 [ {176f 120 |
FLAGELLATE #2 cee otf { (1 6.91 16 | {10.91 TM@ 1
FLAGELLATE #3 ceu off ! 4 1 1112209} 156
FLAGELLATE #4 ceu {1 | 15110031 24 i |
FRUSTULIA RHOMBOIDES cet Il t tt { x tl { i
LYNGBYA LIMNETICA . Fu tl { {3117.6 cn 1 |
MELOSIRA cer sot 1 ! im ! im { x |
MELOSIRA 2 ‘ CEL «131 5.3! 25 tf ! 1 1 !
MERTSMOPEDIA PUNCTATA cor olf { ti { 1 41 ze
NAVICULA cer tf i tt { { {1.31 9 61
NAVICULA ? cee ott ! x tt ! {4 ! |
NITZSCHTA ceL of | 4.31 17 1 Lt 6.9 16 1 1 6.8! 46 1
QPHIGCYTIUM CAPITATUM ? cer of { 2.01 8 ott ' tt I i}


0€

LAKE NAME: MARTIN LAKE
STCRET NUMBER: 0107 \

DATE

MYXCPHYCEAN
CHLOROPHYCEAN
EUGLENOPHYTE
OTATOM
COMPOUND

DATE

GENUS
SPECIES

AVERAGE DIVERSITY

NUMBER OF TAXA

NUMBER OF SAMPLES CCMPOSITED
MAXIMUM DIVERSITY

TOTAL DIVERSITY

TOTAL NUMBER OF INDIVIDUALS/ML
EVENESS COMPONENT

MEAN NUMBER CF INDIVIDUALS/TAXA
NUMBER/ML GF MOST ABUNDANT TAXON

PALMER'S

NYGAARD TROPHIC STATE INDICES

03 18 73

01/0
04/0
0220
0043
09/0

mmamm

06 16 73 08 25 73 11 01 73

2200
1200
0/06
1.00
5250

mmamm

0.80 E 2067 E
1620 E 2033 €
o/10 2 0007 2?
0.86 E
3020 E

ORGANIC PCLLUTICN INDICES

03 18 73 06 16 73 08 25 73 11 01 73

03
00

o1
00

03 10
00 00

SPECIES DIVERSITY AND ABUNDANCE INDICES

DATE

MAX!

ReCzorEenr

03 18 73 06 16 73 08 25 73 11 O01 73

2021
21.00
7200
4.39
5063011
2291.00
0.50
109.10
1300.00

2075
23200
7.00
4052
2838.00
1032.00
0.51
44087
454200

3.68 3.74
35.00 37.00
7200 7200
5.13 5.21

1924640 10475274
5230.00 2801.00
0.72 0.72
149.43 15670
1208.00 623.00


et

LAKE NAME: BANKHEAD LAKE
STCRET NUMBER: O1OL

NYGAARO TROPHIC STATE INDICES
DATE 06 08 73 08 25 73 10 30 73

MYXOPHYCE AN 2050 E O5/0 E 0050 E
CHL ORCPHYCEAN 3200 E O7/O E 3.00 E
EUGL ENOPHYTE Oe27 E 0.25 E 0029 E
OIATCM STE 0.55 & 0050 E
CCMPQUND 9.00 E 21/0 — 6.00 E

PALMER'S ORGANIC POLLUTICN INDICES
DATE 06 08 73 08 25 73 10 30 73

GENUS 04 08 05
SPECIES 00 00 04

SPECIES OIVERSITY AND ABUNCANCE INDICES
DATE 06 08 73 08 25 73 10 30 73

AVERAGE DIVERSITY H 2094 3.19 3024

NUMBER OF TAXA Ss 30.00 38.00 30.00

NUMBER OF SAMPLES COMPOSITED “ 4.00 4200 4.00
MAXIMUM DIVERSITY MAXH 4e91 5025 4e91

TOTAL DIVERSITY D 4154.22 3690.83 3123-36

TOTAL NUMBER OF INDIVIDUALS/ML N 1413.00 1157.00 964.00
EVENESS CCMPONENT J 0060 0061 0066

WEAN NUMBER CF INDIVIDUALS/TAXA L 47010 30045 32013
NUMBER/ML OF MOST ABUNDANT TAXON K 554200 476.00 387200


QUALITY CONTROL

Internal quality control checks on species identifications and
counts were performed on a regular basis between project phycologists
at the rate of 7 percent. Although an individual had primary
responsibility for analyzing a sample, taxonomic problems were
discussed among the phycologists.

Additional quality control checks were performed on the Survey
samples by Dr. G. W. Prescott of the University of Montana at the rate
of 5 percent. Quality control checks were made on 75 percent of these
samples to verify species identifications while checks were made on
the remaining 25 percent of the samples to verify genus counts.
Presently, the agreement between quality control checks for species
identification and genus enumerations is satisfactory.


Le

LAKE NAME: LAY LAKE CONTINUED
STORET NUMBER: 0106

06 06 73 08 29 73 10 31 73

! ALGAL ALGAL | ALGAL sf

! UNITS =I UNITS = UNITS |
TAXA FORM {[S %C PER ML [S %C PER ML [S %C PER ML |
ACTINASTRUM HANTZSCHIT 11 t tt { tt ' t
Ve FLUVIATILE cor ott ! {| 10f 127 IS ! x ot
ANABAENA FIL ft 1 0.31 cS ee ! (1 tert 40 4
ANABAENA #1 Fre is { ( {0.31 42 (1 i {
ANABAENA #2 Fro 1 (3/1406] 1856 [I ' !
ANABAENOPSIS FIL otf ! 1 f 0.7t 84 ot | !
ANKISTROOESMUS 2 cee tt 1 tt ! 1 | 0.6 20)
CENTRIC DIATOM #1 cer ott { im i x tft { !
CHLAMYDOMONAS cet of | ! im) ' ff 2038 a1 of
CHLCROGCNTUM ceu ott { tt ' x tt { '
CHLORCPHYTAN FLACELLATE cet ft i x t tt ! {
CLCSTERTUM cet ot] 1 1 1 0031 42 lt { {
COCCCNEIS PLACENTULA ti ! imi | tt ! i
Ve EUGLYPTA cet of ft if ti 1 11 o.6f 20 641
COELASTRUM MICROPORUM cor ott 4 x tt ' it 1 1
CCELASTRUM MICROPORUM ? co Of ! im t im i x t
CRUC IGENIA APICULATA cor ft { tt ! 1 1 oe6f 20 (1
CRUCIGENIA TETRAPEDIA cou ft 1 x 1 1 0.31 42 4 { x ft
CRYPTCMCNAS cet «of2l 83! 0777 | it ! x ot
CRYPTOMONAS #1 cer of 1 141 500] 633 [1f15e31 546
CYANOPHYTAN COLCKY co. ff 1 x tl ! tt { 1
CYCLOTELLA cer ott 1 x tf i ie { |
CYCLOTELLA MENEGHINTANA cer oti | xX [1 401 506 14] 8.5] 303 |
CYCLOTELLA STELLIGERA cee ott { tt 1 tt { x 4
DACTYLOCCCCOPSIS cet 1 ft 1.0f 93° «It I Oe7f a4 1 f 2.31 sr
DICTYOSPFAERIUM PULCHELLUM co olf t ml { x TE 0.6f 20 |
DINOFLACELLATE cee ot | { tt ! ( U o.6t 20 |
DINGFLAGELLATE #1 CEL 1 | 0031 31 ott 1e3! 169 OT { {
CINCFLACELLATE #2 ceL ott 1 x 110.78 a It { 1
OINCFLAGELLATE #3 cet {| O.7t 62 ot 4 if ti { I
EUASTRUM CEL tt { x Ft { x dt { x 1
EUDORINA co ott ( tt t tt ' x 4


ve

LAKE NAME: HOLT LOCK AND DAM CONTINUED
STORET NUMBER: 0105

06 07 73 08 29 73 10 21 73
! ALGAL {| ALGAL I ALGAL
{ UNITS I UNITS [ UNITS

TAXA FORM 1S %C PER ML [IS %C PER ML [S %C PER ML

ACTINASTRUM HANTZSCKIT cot T4liaeat 234 ~ TT 1 11 i !
AMPHIPLEURA PELLUCIDA cee ott { tt { Tt { x ot
ANKISTRODESMUS FALCATUS cee ott ' im ' it ! x 1
ATTHEYA cer sot I \ 14 \ x Ut { 1
CENTRIC DIATOMS ceL ott ! x Tt { 1 1 {
CHLORCGCNI UM cee ot | { 1 { o.7f mio ! '
CLCSTERIUM CEL it ! tt { tt { x 1
CCELASTRUM cor ff { It 2.91 4 it 1 x t
CRUCIGENIA TETRAPEDIA cor otf ! 1 f o.7f wot ' 1
CRYPTOMCNAS #1 CEL «= {1132.0f 521 {1lts.6f 225 I { {
CRYPTOMCNAS #2 ceu ott ! { 1 3.68 56 t '
CYCLOTELLA #1 CEL «151 2641 39° «131 7e31 0 113 st 1 {
CYCLCTELLA #2 cee ot I 1 x Tf Sell 7 14 { !
DACTYLOCOCCOPSIS #1 cee ot I ( ( {11.71 180 FI { !
CACTYLOCOCCOPSIS #2 cer tt { i ! x tt ( {
DIACBRYON cee ot { x if ' x tt 1 i
OINOFLAGELLATE ceu otf 1 Ut 441 6s otf { t
DINOFLAGELLATE SPP. CEL «13f 88! 6143) OE { i { !
EUGLENA ceu ott ! { { 0.71 wu ott { !
FLAGELLATE #1 CEL «(12140081 664 11 | X  L157641 |
FLAGELLATE #2 ceu ot I i] 1 1 2021 00036 | { 1 '
FLAGELLATE #3 cet ott i tt | x ft { !
FLAGELLATES cer ot I 1 (4i1e.21 281 «tI ! I
KIRCENERTELLA cee ot I | im t 13114621 22 st
LYNGBYA LIMNETICA FIL of | { {5 5.1 7m Tt 1 I
MELCSIRA VARTANS cer ot { im { tt { x |
MERISHOPEDIA MINIMA co otf ( im { (41 Teil uot
MICROCYSTIS MINIMA cor 1} { im! i x tt ! {
NAVICULA cet ft ! x tt | td l x |
NAVICULA MINIMA cer ott ! x ft t tt j |
NAVICULA MUTICA 14 | tt { i { 1
Ve TROPICA cert I x tt | 1 | t


INTRODUCTION

The collection and analysis of phytoplankton data were included
in the National Eutrophication Survey in an effort to determine
relationships between algal characteristics and trophic status of

individual lakes.

During spring, summer, and fall of 1973, the Survey sampled 250
Over 700 algal species and varieties were
identified and enumerated from the 743 water samples examined.

lakes in 17 States.

This report presents the species and abundance of phytoplankton
in the 11 lakes sampled in the State of Alabama (Table 1). The
Nygaard's Trophic State (Nygaard 1949), Palmer's Organic Pollution
(Palmer 1969), and species diversity and abundance indices are also

included.
TABLE 1. LAKES SAMPLED IN THE STATE OF ALABAMA

STORET No. Lake Name County

0101 Bankhead Walker

0103 Gantt Reservoir Covington

0104 Guntersville Reservoir Marshall, Jackson

0105 Holt Lock and Dam Tuscaloosa

0106 Lay Lake Chilton, Coosa

0107 Martin Lake Elmore, Tallapoosa

0108 Mitchell Lake Coosa, Chilton

0109 Pickwick Lake Colbert, Lauderdale
(Tishmoningo in MS
& Hardin in TN)

0112 Weiss Lake Cherokee

0114 Wilson Lake Sauderdale, Colbert,
Lawrence

0115 Lake Purdy Jefferson, Shelby



CONTENTS

Foreword .......e008 eee eeeee cS ecneccsenccoeees vue eccenscavccenesece iti
Introduction .........68 cence ccceeseceeteensvcsnesescsosscseeesess
Materials and Methods ......csccececeeee ereeeee rrr errr rr 2

Lake and Site Selection .....cccccceeecececcececerneceeesceeees 2

Sample Preparation ..cscscseseccceecvcnceeeecececeeeesecece 4
Examination .....ccccceceeeecececeeeesseees seeeenceccecescssees 3
Quality Control .....ccceeeseeeeseeeer er eer rrr ret 4
RESUItS ....ceeeeeeeeeee eae n en eeee eee eseenreecesesneseesesscescese OD
Nygaard's Trophic State Indices .......ccceeesseees eevee ececens 5

Palmer's Organic Pollution IndiceS ....ccsseececcseseecescvevee 5

Species Diversity and Abundance Indices ....ccscesccvacescecees 7

Species Occurrence and Abundance .......ceeeeececevevscees ar)
Literature Cited .....ccccceccceeceecnstceeeee seceseces see eeeeeeeee 10
Appendix. Summary of Phytoplankton Data ........see05 seeeee seeeeee Ll


el

LAKE NAME? BANKHEAD LAKE CONTINUED
STCRET NUMBER? 0101

06 08 73 08 25 73 10 30 73

{ ALGAL | ALGAL I ALGAL

! UNITS [| UNITS [ UNITS
TAXA FORM IS %C PER ML |S %C PER ML [S %C PER ML
ACTINASTRUM COL =«f1139-2f 554 ff { tt {
ACTINASTRUM HANTZSCHII 11 tt im {
Ve FLUVIATILE cor of I { x tl l tt {
ANABAENA FIL of f elf we tt | tt !
ANKISTRODESMUS ? cee of | 62t ss ott 1 tt |
ANKISTRCOESMUS FALCATUS cer ot I ! ti ' 1 1 o.9f 9
ASTERICNELLA ? CEL «of | O.6t 6 tf { 4 ‘
ATTHEYA cee 11 t tt ( x [1 0.91 9
CENTRIC DIATOM cee ot I ( x at i tt !
COEL ASTRUM cou ft] ‘ ( t0.7] 8 oil !
CCELASTRUM SPHAERICUM cor of I ! {1 1 x tt {
CORONASTRUM cor oft 1 110.7) Cn |
CCSCINCCISCUS CEL tt ' tt | 1 1 0.91 9
CCSMARTUM ce ott 1 im ! { | o.91 9
CRYPTOMONAS cee ot { tt ' {21 4e7t 45
CYCLOTELLA cee ott { x tl ! tl !
CYCLOTELLA MENEGHINI ANA ceu ot I | 1 f 1.4! le tl '
CYCLOTELLA STELLIGERA CEL 131 7641 «2105 tt 14d we ott t
TACTYLCCOCCOPSIS cet ot I 2.8 4o 14 ! tl 1
DINOBRYCN cer ot I x 1 2.01 23 1 1 O91 9
DINOFLAGELLATE #1 cee ot I ! im { 11 1.61 15
CINCFLAGELLATE #2 cee ot ! im t 1 { L.6f 15
OINOFLAGELLATE #3 cee ot I | tt ' 1 1 106! 15
DINOFLAGELLATE #4 cet of ft (O66! 8 ft {0.71 8 it {
CINOFLACELLATE #5 cel of I ! (1 1e4t le of] 1
EUGLENA CEL 151 1671 24 I 1 2001 23 «131 204 23
EUGLENA ACUS ceL sot { i { x it !
EUNCTIA #1 cee ot I | il { x tl {
EUNOTIA #2 ceL sot | ! im { x ft (
FLAGELLATE #1 cee ot iI ( tl { {4l18.of 174
FLAGELLATE #2 ceu ot I { i ( {1140.11 387
FLAGELLATE #3 ceu ot I | (1 1.41 we It i


Prior to examination, the composite samples were concentrated
by the settling method. Solids were allowed to settle for at least 24
hours prior to siphoning off the supernate. The volume of the
removed supernate and the volume of the remaining concentrate were
measured and concentrations determined. A small (8 ml) library
subsample of the concentrate was then taken. The remaining
concentrate was gently agitated to resuspend the plankton and poured
into a capped, graduated test tube. If a preliminary examination of a
sample indicated the need for a more concentrated sample, the contents
of the test tube were further concentrated by repeating the settling
method. Final concentrations varied from 15 to 40 times the original.

Permanent slides were prepared from concentrated samples after
analysis was complete. A drop of superconcentrate from the bottom of
the test tube was placed in a ring of clear Karo Corn Syrup with
phenol (a few crystals of phenol were added to each 100 ml of syrup)
on a glass slide, thoroughly mixed, and topped with a coverglass.
After the syrup at the edges of the coverglass had hardened, the
excess was scraped away and the mount was sealed with clear fingernail
polish. Permanent diatom slides were prepared by drying sample
material on a coverglass, heatjng in a muffle furnace at 400 C for 45
minutes, and mounting in Hyrax . Finally, the mounts were sealed
clear fingernail polish.

Backup samples, library samples, permanent sample slides, and
Hyrax-mounted diatom slides are being stored and maintained at the
U.S. Environmental Protection Agency's Environmental Monitoring and
Support Laboratory-Las Vegas.

EXAMINATION

The phytoplankton samples were examined with the aid of binocular
compound microscopes. A preliminary examination was performed to
precisely identify and list all forms encountered. The length of this
examination varied depending on the complexity of the sample. An
attempt was made to find and identify all of the forms present in each
sample. Often forms were observed which could not be identified to
species or to genus. Abbreviated descriptions were used to keep a
record of these forms (e.g., lunate cell, blue-green filament,
Navicula #1). Diatom slides were examined using a standard light
microscope. If greater resolution was essential to accurately
identify the diatoms, a phase-contrast microscope was used.

After the species list was compiled, phytoplankton were enumerated
using a Neubauer Counting Chamber with a 40X objective lens and a 10X
ocular lens. All forms within each field were counted. The count was
continued until a minimum of 100 fields had been viewed, or until the
dominant form had been observed a minimum of 100 times.

®Registered Trademark


6L.

LAKE NAME? GUNTERSVILLE RES.
STCRET NUMBER: 0104

NYGAARD TROPHIC STATE INDICES

DATE 06 08

MYXOPHY CEAN 6200
CHLOROPHYCEAN 1160
EUGLENOPHYTE 0.12
OLATCM 0.57
COMPCUND 23.0

PALMER'S
DATE 06 08

GENUS
SPECIES

73 08 16 73 «10 22 73

E 4.00 E 6000 E
E 3067 E 5050 E
? 0.13 7 0.09 7
E 1.00 E 0.87 E
€ 1003 E 10.0 E

ORGANIC POLLUTION INDICES
73° 08 16 73: «10 22 73

04 a. 22
03 04 00

SPECIES DIVERSITY AND ABUNDANCE INOICES

DATE 06 08

73° 08 16 73° «10 22 73

AVERAGE DIVERSITY H 3256 4.09 3251

NUMBER OF TAXA Ss 40.00 47.00 48.00

NUMBER OF SAMPLES COMPOSITED TM 8.00 6.00 4200
MAXIMUM DIVERSITY MAXH 5032 555 5058

TOTAL DIVERSITY D 13303272 39382.61 107406.00

TOTAL NUMBER OF INDIVIDUALS/ML N 3737200 9629200 30600200
EVENESS COMPONENT J 067 0n74 0063

MEAN NUMBER CF INDIVIDUALS/TAXA L 93.43 204.87 637.50
NUMBER/ML SF MOST ABUNDANT TAXON kK 845.00 2114.00 9029.00



EPA-600/3-77-082
July 1977 Ecological Research Series



ey

LAKE NAME: WEISS LAKE CONTINUED
STCRET NUMBER: O112

08 25 73 10 30 73
1 ALGAL ff ALGAL
‘ ! UNITS =f UNITS

TAXA FORM {1S &%C PER ML [S$ %C PER ML

ACHNANTHES cee tt 1 x ot
ANABAENA FIL ff ' x |
ANABAENA #1 FIL of f ( x !
ANABAENA #2 Fit { x {
ANKISTRODESMUS FALCATUS cee oF ft aeit 132 455 |
CERATIUM ce oft ! 3e of
CHLAMYOCMONAS cn { x {
CLCSTERIUM ceL ff 0.5} 66 x ot
COCCCNETS PLACENTULA tt | !
Ve EUGLYPTA cer oT ft ' x !
CCELASTRUM cou =} f 0.5t 66 {
COSMARTUM cee ot { x 38 it
CCSMARTUM #1 ce V1 aeit 132 {
CPRUCIGENIA APICULATA cou ot ft 065d 66 tw 4
CRUCIGENTA TETRAPEDIA cor ff t 38
CRY PTOMONAS cer ott 1 228 it
CYCLCTELLA cee ot | | x |
CYCLOTELLA #1 CEL 14] Tet] 6858 9490
CYCLOTELLA MENEGHINIANA cer =f I 0.5] 66 !
CYCLCTELLA STELLIGERA cet oft ( 342 I
DACTYLOCOCCOPSIS ceL «of | 1.6! 198 1252
DICTYOSPHAERIUM FULCKELLUM cor oI | 38 |
DINOFLAGELLATE CEL of I elf = 132 !
EUASTRUM cee ot ! x 1
FUCLENA cee ot I ! x 14
FLAGELLATE #1 CEL of | 207! = 330 228
FLACSLLATE #2 cee sot | 3s)
FLAGELLATES CEL «13410091 1220 loze |
KIRCHNERTELLA ceL off tell = 132 {
KIRCHNERTELLA SUBSOLITARIA cer ot 4 { x
LYNG BYA FIL ott 1 x x 4
LYNGBYA LIMNETICA FIL fF f 651 792 228



TABLE 2. NYGAARD'S TROPHIC STATE INDICES ADAPTED FROM HUTCHINSON (1967)

Index Calculation Oligotrophic Eutrophic
Myxophycean Myxophyceae 0.0-0.4 0.1-3.0
Desmideae
Chlorophycean Chlorococcales 0.0-0.7 0.2-9.0
Diatom Centric Diatoms 0.0-0.3 0.0-1.75
Pennate Diatoms
Euglenophyte Euglenophyta 0.0-0.2 0.0-1.0
Myxophyceae + Chlorococcales
Compound Myxophyceae + Chlorococcales + 0.0-1.0 1.2-25
Centric Diatoms + Euglenophyta
Desmideae

TABLE 3. ALGAL GENUS POLLUTION INDEX TABLE 4. ALGAL SPECIES POLLUTION

(Palmer 1969) INDEX (Palmer 1969)
PoTTution PoTTution
Genus Index Species Index
4nacystts 1 Anktstrodesmus fateatus 3
Ankistrodesmus 2 Arthrospira jennert 2
Chlamydomonas 4 Chlorella vulgaris 2
Chlorella 3 Cyclotella meneghiniana 2
Clostertun 1 Euglena gracilis 1
Cyelotella 1 Euglena viridis 6
Euglena 5 Gomphonema parvulum 1
Comphonema. 1 Melosira vartans 2
Lepocinelis 1 Navicula eryptocephala 1
Melosira 1 Nitaschta acicularis 1
Micractiniun 1 Nitzschia palea 5
Navicula 3 Osctllatorta chlorina 2
Nitzschia 3 Osetllatoria Limosa 4
Oscillatoria 5 Osetllatoria princeps 1
Pandorina 1 Oseillatoria putrida 1
Phacus 2 Oscillatoria tenuis 4
Phormidium 1 Pandortna morum 3
Scenedesmus 4 Scenedesmus quadricauda 4
Stigeoelontum 2 Stigeoelonium tenue 3
Synedra 2 Synedra ulna 3



RESULTS

The Appendix summarizes all of the phytoplankton data collected
from the State by the Survey. It is organized by lake, including
an alphabetical phytoplankton species list with concentrations for
individual species given by sampling date. Results from the application
of several indices are presented (Nygaard's Trophic State, Palmer's
Organic Pollution, and species diversity and abundance). Each lake
has been assigned a four digit STORET number. [STORET (STOrage
and RETrieval) is the U.S. Environmental Protection Agency's computer
system which processes and maintains water quality data.] The first
two digits of the STORET number identify the State; the last two
digits identify the lake.

NYGAARD'S TROPHIC STATE INDICES

Five indices devised by Nygaard (1949) were proposed under the
assumption that certain algal groups are indicative of levels of
nutrient enrichment. These indices were calculated in order to
aid in determining the surveyed lakes' trophic status. As a general
rule, Cyanophyta, Euglenophyta, centric diatoms, and members of
the Chlorococcales are found in waters that are eutrophic (rich
in nutrients), while desmids and many pennate diatoms generally
cannot tolerate high nutrient levels and so are found in oligotrophic
waters (poor in nutrients).

In applying the indices to the Survey data, the number of taxa
in each major group was determined from the species list for each
sample. The ratios of these groups give numerical values which can
be used as a biological index of water richness. The five indices
and the ranges of values established for Danish lakes by Nygaard
for each trophic state are presented in Table 2. The appropriate
symbol, (E) eutrophic and (0) oligotrophic, follows each calculated
value in the tables in the Appendix. A question mark (?) was entered
in these tables when the calculated value was within the range of
both classifications.

PALMER'S ORGANIC POLLUTION INDICES

Palmer (1969) analyzed reports from 165 authors and developed
algal pollution indices for use in rating water samples with high
organic pollution. Two lists of organic pollutiontolerant forms
were prepared, one containing 20 genera, the other, 20 species (Tables
3 and 4). Each form was assigned a pollution index number ranging
from 1 for moderately tolerant forms to 6 for extremely tolerant
forms. Palmer based the index numbers on occurrence records and/or
where emphasized by the authors as being especially tolerant of
organic pollution.


8€

LAKE NAME: PICKWICK LAKE
STCRET NUMBER: 0109

NYGAARD TROPHIC STATE INCICES
DATE 06 09 73 08 15 73 1O 22 73

MYXOPHYCEAN o/o C 05/0 E 02/0 £
CHLOROPHYCE AN 04/0 E OS/0 E O6/0 E
EUGLENOPHYTE 0/04 2 0/10 2 o/cs 2
OIATOM 1.00 E 0.67 E 4.00 E
COMPOUND 09/0 E 14/0 E 12/0 E

PALMER'S CRGANIC POLLUTICN INDICES
DATE 06 09 73 08 15 73 10 22 73

GENUS ol 02 ol
SPECIES 00 00 00

SPECIES DIVERSITY AND ABUNDANCE INCICES
DATE 06 09 73 08 15 73 10 22 73

AVERAGE DIVERSITY H 2016 2285 1.35

NUMBER OF TAXA s 18200 25200 15.00

NUMBER OF SAMPLES CCMPOSITED M 4200 4200 3.00
MAXIMUM DIVERSITY MAXH 4.17 4064 4.25

TOTAL DIVERSITY 1) 2805084 3129230 3504.60

TOTAL NUMBER OF INDIVIDUALS/ML N 1299.00 1098200 2596.00
EVENESS COMPONENT J 0.52 0.61 0.32

MEAN NUMBER CF INDIVIDUALS/TAXA L T2017 43.92 136.63
NUMBER/ML OF MOST ABUNDANT TAXON K 457200 335200 1299.00


92

LAKE NAME: LAY LAKE
STORET NUMBER: 0106

NYGAARD TROPHIC STATE INDICES
DATE 06 06 73 08 29 73 10 31 73

MYXCPHYCEAN 5200 E 4200 E 4.00 E
CHLOROPHYCEAN 1300 E 9050 E 15.0 E
EUGLENOPHYTE 0.06 ? 0.04 ? 0.05 2?
OIATOM 2.00 E 0.67 E LITE
COMPOUND 27.0 E 1700 E 2760 E

PALMER'S ORGANIC POLLUTION INDICES
DATE 06 06 73 08 29 73° 10 31 73

GENUS os 09 15
SPECIES 04 06 06

SPECIES DIVERSITY AND ABUNDANCE INDICES
DATE 06 06 73 08 29 73 10 31 73

AVERAGE DIVERSITY 2.91 3.59 3.39

NUMBER OF TAXA 40.00 53200 41.00

NUMBER OF SAMPLES CCMPOSITED 3200 3.00 3200
MAXIMUM DIVERSITY MAX 5.32 5273 5235

TOTAL OIVERSITY

TOTAL NUMBER OF INDIVIDUALS/ML
EVENESS COMPONENT

MEAN NUMBER OF INDIVIDUALS/TAXA
NUMBER/ML OF MOST ABUNDANT TAXCN

27223005 45718665 12126.03
9355200 12735200 3577200
0.55 0.63 0.63
233288 240.29 87224
3109200 4049.00 910.00

Aeezorenr


fv

LAKE NAME: WEISS LAKE
STCRET NUMBER: 0112

TAXA

MELOSIRA

WELOSIRA #2

MELOSIRA #4

MELGSIRA #5

MELOSIRA DISTANS
WERISMOPEDIA TENUISSIMA
MICROCYSTIS AERUGINOSA
MICROCYSTIS INCERTA
NAVICULA #1

NAVICULA #2

NITZSCHIA

NITZSCHIA #2
OSCILLATCRIA #1
CSCILLATCRIA LIMNETICA
PANDORINA MORUM
PEDIASTRUM CUPLEX

Ve RETICULATUM
PENNATE CIATOM #1
PENNATE OFATOM #4
PHACLS

PINNULARTA VIRIOIS
PLATYDCRINA CAUCATUM
PTEROMONAS
RAPHIDICPSIS
SCENEDESMUS
SCENEDESMUS #1
SCENEDESMUS #2
SCENEDESMUS #3
SCENEDESMUS ABUNDANS
SCENEDESMUS ACUMINATUS
SCENEDESMUS DENTICULATUS
Ve FENESTRATA

CONTINUED
08 25 73 10 30 73

( ALGAL | ALGAL I

i UNITS ff UNITS
FORM !s &%C PER ML 1 zc PER ML |
CEL «151 491 594 OT I 1
cee of | uth 6132) fF t2) sz ft
ce tt t Ist 5.21 645 |
ceL sot J 1 x wf 1 i
CEL 2425.5) 3103) 1142.41 5275 I
cou oO | 2671 «63300 AE O66d |
ccu tl ' tt 1 x '
coL tt t tt 1 x of
cer ott ! x ttl ! x |
CEL it | ft 1 x '
cEL 1 6004 126 {i t 4
CEL i] 1 xX 1 F 0631 38
FIL] [1{13.01 1585 tf I !
FIL of 4 { {1 O06! tm 1
cou tf { x € 1 063! 38 dt

11 1 it | '
cot 11 1 tt 1 x 4
cEL 1 1.64 198 tt i t
cee oI I 1 x Ft I {
cau 4 1 1 t 0.31 38°C
cer if ( tt { x 4
coL tt 1 x tt ! |
cer otf { 1 1 0031 38 Of
FIL of} $4.3! 528 tI l {
cor Il 1 x ot 4 i
COL | |} 22h 264 ft 0061 we 4
cor 1 | 0.58 66 1 { 0.68 me 1
co ott ( (1 0.34 38 Cit
cou off lett «132 [1 O09f 114 I
co ott 1 x tt 1 x |

td 1 td i} 1
cor ott ' x tt ! !


G2

LAKE NAME: HOLT LOCK AND DAM CONTINUED
STORET NUMBER: 0105

06 07 73 08 29 73 1o 31 73
1 ALGAL ALGAL Ot ALGAL
! UNITS = [ UNITS = | UNITS
TAXA FoRM |S %C PER ML {[S %C PER ML |S %C PER ML
NITZSCHIA cet of | i] fl { {5{ Tell i {
OSCILLATORIA Fi otf { {( o.7f wu ott { {
CSCILLATORIA ANGUSTA FIL tt i {1 0271 1 ott t t
PANOORINA MGRUM cor oft { x tt ! tt { !
PENNATE OLATOM #1 ceu ott { tt ! x tt ! |
PENNATE DIATOM #4 cee ott ! x Ft 2.21 34 61 dt ! |
RHIZCSOLENIA cee ott 1 {f { x tl { {
SCENEDE SMUS ABUNDANS cor otf | 11 1.5 23 «11 { {
SCENEDESMUS QUADRICAUDA cor ff t { {2.21 34 «(2114.24 22 1
STAURASTRUM cer ott { 1 1.51 23 61 I 1 {
SYNEORA #1 ceL «{ [ 1661 ze (tt { im ! (
SYNEORA #2 cee oft [ {2i 8.7f 135 Tt { '
SYNEDRA #3 ceL ot I ! 1 t 2.21 34 (Cd | 1
SYNEDRA FASCICULATA tt 1 1 ! tt ' 1
Ve TRUNCATA cee ott i} 14 2.91 45 1 i}

TOTAL 1627 1543 155


MATERIALS AND METHODS

LAKE AND SITE SELECTION

Lakes and reservoirs included in the Survey were selected through
discussions with State water pollution agency personnel and U.S.
Environmental Protection Agency Regional Offices (U.S. Environmental
Protection Agency 1975). Screening and selection strongly emphasized
lakes with actual or potential accelerated eutrophication problems.

As a result, the selection was limited to lakes:

(1) impacted by one or more municipal sewage treatment plant
outfalls either directly into the lake or by discharge to an
inlet tributary within approximately 40 kilometers of the
Take;

(2) 40 hectares or larger in size; and
(3) with a mean hydraulic retention time of at least 30 days.

Specific selection criteria were waived for some lakes of particular
State interest.

Sampling sites for a lake were selected based on available
information on lake morphometry, potential major sources of nutrient
input, and on-site judgment of the field limnologist (U.S.
Environmental Protection Agency 1975). Primary sampling sites were
chosen to reflect the deepest portion of each major basin in a test
lake. Where many basins were present, selection was guided by
nutrient source information on hand. At each sampling site, a depth-
integrated phytoplankton sample was taken. Dapth-integrated samples
were a uniform mixture of water from the surface to a depth of 15 feet
(4.6 meters) or from the surface to the lower limit of the photic zone
representing 1 percent of the incident light, whichever was greater.
If the depth at the sampling site was less than 15 feet (4.6 meters),
the sample was taken from just off the bottom to the surface.
Normally, a lake was sampled three times in 1 year, providing
information on spring, summer, and fall conditions.

SAMPLE PREPARATION

Four milliliters (m1) of Acid-Lugol's solution (Prescott 1970)
were added to each 130-ml sample from each site at the time of
collection for preservation. The samples were shipped to the
Environmental Monitoring and Support Laboratory, Las Vegas, Nevada,
where equal volumes from each site were mixed to form two 130-ml
composite samples for a given lake. One composite sample was put into
storage and the other was used for the examination.

2


OL

LAKE NAME: GANTT RES.
STCRET NUMBER: 0103

NYGAARD TROPHIC STATE INCICES
DATE 06 19 73 08 30 73 11 03 73

MYXCPHYCEAN 1.33 & 4.00 £ 1.50 E
CHLOROPHYCEAN 1.33 E 0/01 0 1.50 E
EUGLENOPHYTE 0025 E 0.75 E O17 ?
DIATOM 0.80 E Ool? 2? 0060 E
COMPOUND 4.6T E 8.00 E 5.00 €

PALMER*S ORGANIC POLLUTICN INDICES
DATE 05 19 73 08 30 73° LL 03 73

GENUS 00 00 00
SPECIES oo 00 00

SPECIES DIVERSITY AND ABUNCANCE INDICES
DATE 06 19 73 08 30 73 11 03 73

AVERAGE DIVERSITY 2099 3064 3020

NUMBER OF TAXA 26.00 20.00 24.00

NUMBER OF SAMPLES CCMPOSITED 3200 3000 3200
MAXIMUM DIVERSITY MAX! 4070 4032 4058

TOTAL DIVERSITY
TOTAL NUMBER OF INOIVIOUALS/ML

1178.06 848.12 2176.00
394.200 233.00 680.00

ROCZOFEMT

EVENESS COMPONENT 0.064 0084 0.70
MEAN NUMBER CF INDIVIDUALS/TAXA 15015 11665 28.33
NUMBER/ML OF MOST ABUNDANT TAXON 170200 41200 156200


€€

LAKE NAME: MARTIN LAKE CONTINUED
STCRET NUMBER: 0107

03 18 73 06 16 73 0g 25 73 11 01 73

| ALGAL Of ALGAL I ALGAL | aALGAL ot

1 UNITS = [ UNITS { UNITS [ UNITS 1

TAXA FORM {1S %C PER ML 1S 2C PER ML 1S 8C PER ML {S 2C PER ML |
STAURASTRUM #2 cet ott ( 1 1 O.6f 6 43f 5e2t 0 271 28 tl
STAURASTRUM #3, . ce ott { tt 1 (0.91 ce) x ot
STEPHANCOT SCUS cee ott 1 i ! { f 2.8f 148 !
STICHOCCCCUS 7 Fie tt { tt 1 tt ! x 1
SYRECOA cee ott 1 1 t o.st es tt i !
SYRECRA #1 cer ot 1 it ' 11 1.41 4 x 4
SYNECRA #2 cee ot ' tt ' tt i.st 99 142
TABELLARTA ce ott { xX ft 20st 2 tt i i
TETRAEDRCA MINT MUM cee ot 1 it t {1 c.st 25 x ot
TETRAECAON TRIGCNUM cee ott { tt 1 it | x i}
TRACKELCMCNAS cet tt ! x ott 1 i i !
TREUBER TA cee ott 1 tt 4 x ote t 1

TOTAL 2291 1032 5230 2801


ve

LAKE NAME: MITCHELL LAKE
STCRET NUMBER: 0108

NYGAARD TROPHIC STATE INDICES
DATE 06 06 73 08 29 73 10 31 73

MYXOPHYCEAN 22000 E 9.00 E 4.00 E
CHLORCPHYCEAN 3050 € 10.0 E 13.0 E
EUGLENOPHYTE O/11 2 0.05 2? 0.18 2
DIATOM 1640 E 0286 E 0057 E
COMPCUND 9000 E 2620 & 2400 E

PALMER'S ORGANIC POLLUTION INDICES
DATE 06 06 73 08 29 73 10 31 73

GENUS ol 09 09
SPECIES 00 04 06

SPECIES DIVERSITY AND ABUNDANCE INDICES

DATE 06 06 73 06 29 73 10 31 73

AVERAGE DIVERSITY 3242 3055 3219

NUMBER OF TAXA 33200 39200 39.200

NUMBER OF SAMPLES COMPOSITED 3.00 3.00 3200
MAXIMUM OIVERSITY MAXH 5004 5029 9

TOTAL DIVERSITY

TCTAL NUMBER OF INDIVIDUALS/ML
EVENESS COMPONENT

MEAN NUMBER OF INDIVIDUALS/TAXA
NUMBER/ML OF MOST ABUNDANT TAXON

11887692 1700805 8638052
3476200 4791200 2708.00
468 0.67 0260
10°.33 122.85 69444
782200 1459200 $48.00

AerezorEwr


60

LAKE NAME: LAKE PURDY
STCRET AUMBER: 0115

NYGAARD TROPHIC STATE INDICES
DATE 08 30 73 «11 02 73

MYXOPHYCEAN 4000 E 3000 E
CHLORCPHYCEAN 2067 E 3033 £
EUGL ENOPHYTE 5 2 0.05 2
OLATOM 200 E 0050 E

E

CC#PCUND 8.00 E Te6T

PALMER*S ORGANIC POLLUTION INDICES
DATE 08 30 73 11 02 73

GENUS 10 ll
SPECIES 03 00

SPECIES DIVERSITY AND ABUNDANCE INDICES
DATE 08 30 73 «11 02 73

AVERAGE OIVERSITY H 3252 3223

NUMBER OF TAXA s 35-00 39200

NUMBER OF SAMPLES COMPOSITED M 3200 3.00
MAXIMUM DIVERSITY MAXH Sel3 5229

TOTAL DIVERSITY D 14280264 16437247

TCTAL NUMBER OF INCIVIDUALS/ML N 4057200 5089200
EVENESS COMPONENT J 9069 Oe 61

MEAN NUMBER OF INDIVIDUALS/TAXA t 115.91 130.49
NUMBER/ML OF MOST ABUNDANT TAXON K 1077.00 1890.00


2

LAKE NAME: GUNTERSVILLE RES.
_ STCRET NUMBER: 0104

TAXA

PANDORINA MORUM
PEDIASTRUM BORY ANUM
PEDIASTRUM DUPLEX
PECIASTRUM CUPLEX

Ve GPACILIMUM
PECIASTRUM SIMPLEX
PENNATE DIATOM
PENNATE DIATOM #1
PENNATE DIATOM #2
PENNATE OLATOM #3
PENNATE DIATOM #4
PHACUS

PHACLS PYRUM
RAPHIOIOPSIS
RAPHICIOPSIS MEODITERRANEA
RHIZOSCLENIA ERITENSIS
SCENEDESMUS
SCENEDESMUS #1
SCENEDESMUS #2
SCENEDESMUS ABUNCANS
SCENEDESMUS ACUMINATUS
SCENEDE SMUS QUADRICAUDA
STAURASTRUM
STAURASTRUM #2
STEPHANODI SCUS
SURIRELLA

SYNEDRA #1

SYNECRA ULNA
TETRAEORCN CAUDATUM
Ve LONGISPINUM
TETRAEDRON MINIMUM
TETRAEDRON MUTICUM

TOTAL

CONTINUED
06 08 73 08 16 73 10 22 73

t ALGAL ALGAL of ALGAL

{ UNITS = UNITS | UNITS =
Form | Bc PER ML IS 2C PER ML [S %C PER ML |
cor ff { x tf i] x td 1 x ot
co tt { x tt 1 iat { i
cou ft | x tt 1 tt { 1

tt { tt 1 tt { 1
cor of I ! tt ! tt | x |
cou { { o.7] 26 6d | xX tf 0041 los {
cel of I 1 tt { [ (064) 108 |
cer ot | if x tft { ti 1 1
CEL Tt 1.4! 51 | { td i 1
cel OT ft { { 1 o.9f go tf 1 1
cer ott | 1 | O09 so (tt { x |
cer ott 1 tt { x tf | {
cee oft { 11 1 x tt ! x ot
Fit oft { 11 8.91 855 Ff 18f 537 I
Fir otf { 11 1 x 4 1 {
cet ott t tt { td 1 x ot
coL tt ( tt 1 tt i x ot
co off { x 1 1 O09f go It 1 {
cor olf | tt { { 1 0.41 10st
cou tt t x 11 0.94 90 1 1 0.41 108 !
cou i { 1 { 0051 45 [1 Oo7! 215 1
cor tl { x Tt 1.9! iso of 1 {
cEL tt ! x td ! tt 1 {
cet ott | tt 1 x tt { !
cet ott { 1 ' x It { x 4
CEL tt ( tt { i { x t
CEL of I Ge2])0 230s fa22eof 2114 «{ f 53! 1612 I
ceL 11 1.4! 51 tt 1 i ( {

1 i] tt | tt { (
cet tf | it 1 tt 1 xd
ceL ot It 1.41 5. ott i4t 135 EI 1 x |
ceL | t it t x td t 1

3737 9629 30600


TECHNICAL REPORT DATA
(Please read Instructions on the reverse before completing)

7. REPORT NO, 2.

EPA-600/3-77-082

3. RECIPIENT'S ACCESSIONNO.

4. TITLE AND SUBTITLE

DISTRIBUTION OF PHYTOPLANKTON IN ALABAMA LAKES

5. REPORT DATE

duly 1977

6. PERFORMING ORGANIZATION CODE

7, AUTHOR(S) .
W.D. Taylor, F.A. Hiatt, S.C. Hern, J.W. Hilgert, V.W.
Lambou, F.A. Morris, R.W. Thomas, M.K. Morris, L.R. Wil

jams

8. PERFORMING ORGANIZATION REPORT NO.

9. PERFORMING ORGANIZATION NAME AND ADDRESS

Environmental Monitoring and Support Laboratory 1BA608
Office of Research and Development
U.S. Environmental Protection Agency
Las Vegas, NV 89114

70, PROGRAM ELEMENT NO,

71, CONTRACT/GRANT NO,

12. SPONSORING AGENCY NAME AND ADDRESS

U.S. Environmental Protection Agency-Las Vegas, NV
Office of Research and Development

Environmental Monitoring and Support Laborator

Las Vegas, NV 89114 Y EPA/ 600/07

13. TYPE OF REPORT AND PERIOD COVERED

03-07-73 to 11-14-73

74. SPONSORING AGENCY CODE

% ‘SUPPLEMENTARY _NOTES

reviously released in limited distribution as No. 677 in the Working Paper Series
for the National Eutrophication Survey.

76. ABSTRACT

This is a data report presenting the species and abundance

Results from the calculation of

of phytoplankton in the 11 lakes sampled by the National Eutrophication
Survey in the State of Alabama.
several water quality indices are also included (Nygaard's Trophic

State Index, Palmer's Organic Pollution Index, and species diversity
and abundance indices).

n7.

KEY WORDS AND DOCUMENT ANALYSIS

lc. COSATI Field/Group

a. DESCRIPTORS lb. IDENTIFIERS/OPEN ENDED TERMS
*aquatic microbiology Alabama

Jakes lake eutrophication
*phytoplankton Nygaard's trophic indice

water quality

tion indices
Species diversity and
abundance indices

Palmer's organic pollu-

06 C, M
08 H
Ss 13B

UNCLASSIFIED

78, DISTRIBUTION STATEMENT 79. SECURITY CLASS (This Report) 21, NO. OF PAGES
60
RELEASE TO PUBLIC 20. UNCLASSTETED page) 22, PRICE

EPA Form 2220-1 (9-73)



DISCLAIMER

This report has been reviewed by the Environmental Monitoring and
Support Laboratory-Las Vegas, U.S. Environmental Protection Agency,
and approved for publication. Mention of trade names or commercial
products does not constitute endorsement or recommendation for use.

Vi


lt

LAKE NAME: WEISS LAKE
STORET NUMBER: 0112

NYGAARD TROPHIC STATE INCICES
OATE 08 25 73 10 30 73

MYXCPHYCEAN 2067 E 2067 E
CHLOROPHYCEAN 4.67 E 467 E
EUGLENOPHYTE 0.09 ? 0.09 2?
DIATOM 1614 € E
COMPOUND 1006 E €

PALMER*S ORGANIC POLLUTION INDICES
DATE 08 25 73 10 30 73

GENUS 17 20
SPECIES 05 03

SPECIES DIVERSITY AND ABUNDANCE INDICES

DATE 08 25 73 10 30 73

AVERAGE DIVERSITY H 3075 3025

NUMBER CF TAXA s 4 0 46200

NUMBER OF SAMPLES COMPOSITED ” 200 5200
MAXIMUM DIVERSITY MAXH 5.58 5.52

TOTAL DIVERSITY D0 45547050 40462450

TOTAL NUMBER OF INDIVIDUALS/ML N 12146000 12450200
EVENESS COMPONENT J 0.67 0.59

MEAN NUMBER CF INDIVIDUALS/TAXA t 253204 270065
NUMBER/ML OF MCST ABUNDANT TAYON K 3103.00 5275200


6€

LAKE NAME: PICKWICK LAKE CCNTINUED
STCRET NUMBER: 0109

06 09 73 08 15 73 10 22 73
! ALGAL { AugAL ot ALGAL
| UNITS = | UNITS {| UNITS

TAXA FORM {S %C PER ML [S %C PER ML 1S %C PER ML

ACTINASTRUM HANTZSCHIT cou tt ! i ( tt t x 1
ANKISTRCDESMUS FALCATUS cee st tt x tt ot trot x |
ASTERICNELLA FORMOSA tt { tt { i ' i
Ve? cer =[ | ! x tt ! im { {
CENTRIC DIATOMS cee ott { x td ' tt 1 1
CERATI UM cel ot | ( im ' x i ! x |
CHL AMYDOMONAS cee ot I ( 111.20 13 6 ! x 4
COELASTRUM SPHAERTCUM coo of | ! x tt { tt | {
CRYPTOMCNAS #1 cee 4 1 | { tt ' x ot
CYANOPHYTAN FILAMENT FIL otf ' im! ' x tt { \
CYCLCTELLA #1 ceu ot 1 (3f12.9f 142 «I I { {
CYCLOTELLA #2 cel Ot I 141 7.01 7 It { {
CYCLOTELLA MENEGHINIANA ceu ott ( x tt i} tt { x 1
CACTYLCCCCCOPSIS cet ot 4 ! tt 1 x tt i {
CINOFLAGELLATE #1 cet ott I x Td 1)2i an | ! x {
FLAGELLATE #1 cel ot 1 im ! x tl { x 1
FLAGELLATE #4 CEL «ISI 9.21 1z0 061 4 ( tt 1 '
FLAGELLATES CEL «(4212591 337 «641130.51 335 (2150.01 1299 1
FRANCETA CEL tt | tt { tl | x {
GYRO SIGMA cel ot { x tf { im { 1
LYNG BYA Fit I j 11 i {10.51 12 «I
MELOSIRA #2 CEL «= f3ttlell §=6144 «6 I { x tt { x 1
MELOSIRA #4 CEL «(1135621 457 «= {212700l = 297) 11144031 1149
MELOSIRA DISTANS CEL «(f4lle.s! 241 «1 I { lat 2041 62 ot
MERISMOPEDIA MINIMA cou oft ' 151 4.71 52 (5t 2041 62 I
MICROCYSTIS INCERTA cee of I { tt 2041 ze 61 I { {
NAVICULA #1 cee ot { x tt ' t1 { {
NAVICULA #2 cel ott { tt { x td 1 1
NAVICULA SALINAR TUM | { tt | ti { t
Ve INTERMEDIA ce of ' tt \ x a4 { |
NITZSCHTA #1 cet otf { x TT 204! ze tt { |
CSCILLATCRIA LIMNETICA FIL ' (1 1.21 3 O61 I i


SPECIES OCCURRENCE AND ABUNDANCE

The alphabetic phytoplankton species list for each lake, presented
in the Appendix, gives the concentrations of individual
species by sampling date. Concentrations are in cells, colonies, or
filaments (CEL, COL, FIL) per milliliter. An "X" after a species name
indicates the presence of the species on that date in such a low
concentration that it did not show up in the count. A blank space
indicates that the organism was not found in the sample collected on
that date. Column S is used to designate the examiner's subjective
opinion of the five dominant taxa in a sample, based upon relative
size and concentration of the organism. The percent column (%C)
presents, by abundance, the percentage composition of each taxon.


ts

998-Y8-LL61 :301440 ONILNIMd LNAWNEAAOD ‘Se

LAKE NAME: LAKE PURDY CONTINUED
STORET NUMBER: C115

08 30 73 11 02 73

! ALGAL I ALGAL |

i UNITS =f UNITS =|
TAXA Form {S %C PER ML {S$ %C PER ML |
LAGERHEIMIA SUBSALSA ce tI t im !
MELOSIRA #2 CeL «fll 9681 «89397 L2f37e1t {
MERISMOPEDIA PUNCTATA col =(13126.5f 1077 Ff | 0.4] 18 ot
MERISMOPEDIA TENUISSIMA cou «= ISt10671 435 I ' x ot
MICROCYSTIS INCERTA con of I Leal 57 1 1 O07! 35 Ot
NAVICUL cer ott ' x tt ‘ !
NAVICULA #1 cer ott { im ! x ot
NAVICULA #2 cer ott I im 1 x ot
NAVICULA #3 cee ott { tt { x ot
COCYSTIS cer «off 1.91 7% {4 2e1f 106 J
OSCILLATORIA #1 FIL ([2]13-0f 529 {1 2e1f 106 |
QSCILLATCRIA #2 Fie ‘tf 19f 76 tI O64 ie of
PEDIASTRUM SIMPLEX im { im { 1
Ve CUODENARIUM cou {1 { x ott { x ot
SCENEDESPUS col 1 | 065) 19 1 J Leot 53 I
SCHROEDERTA cer off ! 1 1 0041 is |
STAURASTRUM #1 cet of f 0.51 wo ft ! t
STAURASTRUM #2 cet | J 0.51 19 tf | t
STAURASTRUM PINQUE cere ff I 1 1 0041 1s
SYNEDRA cee oI 4 ! x tt { !
SYNEDRA ULNA ti | td i} {
Ve SUBAEQUALIS cet off 164! 57 1 I O64! 1st
TETRAECRCN #1 on oe oe | we tt ' {
TETRAEDRON GRACILE cee ot 1 x 1 ' {
TETRAEDRON MINTMUM : cer sot 1 x U1 1.01 53

TCTAL 4057 5089


APPENDIX. SUMMARY OF PHYTOPLANKTON DATA

This appendix was generated by computer. Because it was only
possible to use upper case letters in the printout, all scientific
names are printed in upper case and are not italicized.

The alphabetic phytoplankton lists include taxa without species
names (e.g., EUNOTIA, EUNOTIA #1, EUNOTIA ?, FLAGELLATE, FLAGELLATES,
MICROSYSTIS INCERTA ?, CHLOROPHYTAN COCCOID CELLED COLONY). When
species determinations were not possible, symbols or descriptive phrases
were used to separate taxa for enumeration purposes. Each name on
a list, however, represents a unique species different from any other
name on the same list, unless otherwise noted, for counting purposes.

Numbers were used to separate unidentified species of the same
genus. A generic name listed alone is also a unique species. A question
mark (7) is placed immediately after the portion of a name which was
assigned with uncertainty. Numbered, questioned, or otherwise designated
taxa were established on a lake-by-lake basis; therefore NAVICULA
#2 from lake A cannot be compared to NAVICULA #2 from lake B. Pluralized
categories (e.g., FLAGELLATES, CENTRIC DIATOMS, SPP.) were used for
counting purposes when taxa could not be properly differentiated on
the counting chamber.

11


EPA-600/3-77-082
July 1977

DISTRIBUTION OF PHYTOPLANKTON IN ALABAMA LAKES

by

W. D. Taylor, F. A. Hiatt*, S. C. Hern, J. W. Hilgert*,
V. W. Lambou, F. A. Morris*, R. W. Thomas,
M. K. Morris*, and L. R. Williams

Monitoring Operations Division
Environmental Monitoring and Support Laboratory
Las Vegas, Nevada 89114

*Department of Biological Sciences
The University of Nevada, Las Vegas
Las Vegas, Nevada 89154

ENVIRONMENTAL MONITORING AND SUPPORT LABORATORY
OFFICE OF RESEARCH AND DEVELOPMENT
U.S. ENVIRONMENTAL PROTECTION AGENCY
LAS VEGAS, NEVADA 89114


LAKE NAME: MARTIN LAKE
STCRET NUMBER: 0107

TAXA

GOLENK INTA

LYNGBYA

LYNGEYA LIMNET ICA
WELCSIRA

MELSIRA 82

MELOSIRA #3

MELOSIPA DISTANS
MERTSMMPEDTA MARSSONIT
MICRACTINIUM

NAVICULA

MBVICULA #1

RAVICULA #2

NAVICULA #3

NITZSCHIA
QSCTLLATOPIA
CSCILLATOR IA LIMNETICA
FEDIASTRUM TETRAS

Ve TETRACOON

FENNATE DIATOM

PENNATE DIATOM @1
PEANATF DIATOM #2
PENNATE CIATOM #3
PENNATE CIATOM #4

PER IOINIUM

PEPICINIUM #1
PERIDIKIUM WISCCNSINENSE
REIZOSOLENIA
SCENEDESMLS
SCENEDESMUS ACUMINATUS
SCENEDESMUS QUACRICAUDA
STAURASTRUM
STAURASTRUM #1

CONTINUED
03 18 73 06 16 73 08 25 73 11 01 73

' ALGAL I ALGAL | ALGAL I ALGAL
! UNITS = { UNITS =f UNITS | UNITS f
Form {S 8C PER ML | SC PER ML IS) %C PER ML IS %C PER ML |
cet 1 11 0.51 25 ! 1 !
FIL 1 i I 1.of ze of
FIL 1 f iis..f 789 1 1 1
CEL 1.81 iw ott 1 i 1 1
cet ! x [| 0.9f 49 I { x
cel 1 (1 1.91 99 { i i}
CEL 1 x Eb 3e3f 173 212202) 623)
cet ! ti 1 1 1.0f 28}
coL 1 tt { ! 1 '
cet { x tl { x { { {
cEL { tt 1 ! i} i}
CEL ' 4 1 ! ' |
ceL ' tt ! | 1 1
CEL 1 tt 1 1 ' '
FIL 1.21 wi ott 1 1 ! !
FIt 1 tt ! i ! x 1
1 tt 1 i 1 1
cot 1 tt 1 4 1 x ot
cet 1.21 wz ott i i t !
cel 1 1 ft le9t 99 ! 1 {
ceL i 11 1.4! 4 ! a5 |
CEL 1 rt 1 x | x i]
CEL 1 {1 le4d 4 t 28
cet 1 tt ! ( ze |
ceL 5.61 set 1 t '
CEL 7.41 7 12) 421 222 1 i
ceEL { x tt ' x | sy
cot Ooo! 6 Tt O65! 25 4 !
cot { tt ! { 28064
cou 1 tt 1 | 2
CEL 1 It 1 x 1 1
CEL l tt 2e8t 148 1 (



02

LAKE NAME: GUNTERSVILLE RES. CONTINUED
STORET NUMBER: 0104

0s 08 73 08 16 73 lo 22 73
! ALGAL I accaL ot ALGAL
{ UNITS | UNITS = { UNITS
TAXA FORM IS %C PER ML |S &%C PER ML 1S &C PER ML

i}

{

{
ACTINASTRUM HANTZSCHIT tt TT tT 1
Ve FLUVIATILE cou of tot 26 It i ‘
ANABAENA #1 FIL 1 t 2078 1o2 | 1 1.9] 1so 1 i}
ANABAENA #2 FIL of 1 1 x ft { tt |
ANABAENA #3 FIL tt { ti { tt x |
ANABAENA #4 FIL tt 1 ti ! tt x 1
ANABAENOPSIS FIL otf { 1 6e1f sa5 tt I
ANABAENCPSIS #1 FIL ot 1 tt i {3ttt.1) 3404 |
ANABAENCPSIS #2 Fir ott i im ' 1 1 004 10s f
ANKISTRODESMUS FALCATUS cer otf { im { {i x 1
ASTERICNELLA CeL off 2.7f 102 tt I im {
CARTERIA ceu of I 1e4d 5s. ott { 1 {1.4 430 |
CENTRIC OILATOMS cer oI { ti | 11 1.4 430 «I
CHLAMYDOMONAS cee ot { (ft 1.4f 135 ft I
CHLCROGCNI UM ceL of ft 1.4] si tft { x tt {
CHROOCOCCUS cor ff { Ptist 135 Ub 1.8 537 |
CLCSTERIUM cel oft 1 rt { x td {
COELASTRUM SPHAERICUM co ott 1 x tt ! x tt '
CCSMARIUM cer ot I tf ost 45 4 f I
CCSMARIUM #1 cee ot tl | tl 1 tt x ot
COSMARIUM #2 cer sof I | im | 1 1 004 los
CRUCIGENTIA CRUCIFERA co oft { 1 t x Uf !
CRYPTOMGNAS cee ott I i ! x tt t 1
CRYPTOMONAS #1 CEL «121 6621 «9230 «(2 6.51 630 «1 9.8f 3009 J
CRYPTOMCNAS #2 CEL «131 3e4f «0128 ot I t im { ‘
CYCLOTELLA #1 cer {tf ett 77, 1 f 0.91 90 tt test 537
DACTYLOCOCCOPS IS cert t {1 3.7f 390 | 1 8.8f 2687 |
DACTYLOCCCCOPSIS 2 #2 cee ot { x tt ! im { {
DACTYLOCCCCOPSIS #1 ceu of [ 27] toz If { i t (
DICTYCS PHAERIUM cor {1 i} it 1 x td ‘ |
DINCFLAGELLATE #1 cer ot | { x If 0.91 so tt | x 4
OINOFLAGELLATE #2 cer ott i} xX $1 2031 225 1 1 004f 108 |


RESEARCH REPORTING SERIES

Research reports of the Office of Research and Development, U.S.
Environmental Protection Agency, have been grouped into five series. These
five broad categories were established to facilitate further development and
application of environmental technology. Elimination of traditional grouping
was consciously planned to foster technology transfer and a maximum
interface in related fields. The five series are:

Environmental Health Effects Research
Environmental Protection Technology
Ecological Research

Environmental Monitoring

7 PF OD >

Socioeconomic Environmental Studies

This report has been assigned to the ECOLOGICAL RESEARCH series. This
series describes research on the effects of pollution on humans, plant and
animal species, and materials. Problems are assessed for their long- and
short-term influences. Investigations include formation, transport, and
pathway studies to determine the fate of pollutants and their effects. This work
provides the technical basis for setting standards to minimize undesirable
changes in living organisms in the aquatic, terrestrial, and atmospheric environments.

This document is available to the public through the National Technical
Information Service, Springfield, Virginia 22161.


vb

LAKE NAME: WEISS LAKE CCNTINUED
STORET NUMBER? 0112

08 25 73 10 30 73
' ALGAL I ALGAL
1 UNITS =| UNITS
TAXA FORM {IS %C PER ML |S %C PER ML
SCENEDESMUS GRANULATUS ti 1 tt 1 1
Fe DISCIFORMIS cor ft | x 1 ' !
SCHROEDERIA SETICERA cet oft { x 1 ( 1
STE PHANOCT SCUS Ce of EF lett) =61320 «I ! 1
SURIRELLA cel OTT } x ot) t !
SYNECRA cee Ot I | 11 0.31 38d
SYNEORA #1 cer ot ! 11 46f 569 |
TETRAEDRON #1 cer ot | 1 1 0031 38 it
TETRAEDRON MINIMUM cer otf if x af | !
TETRAEORCN MINT MUM toot Vt { {
Ve SCROBICULATUM cer st it ! tt 0.31 38
TETRASTRUM KETERACANTHUM cou of I Get oo 6tl { x ot
TRACHELCMCNAS cer ott 1 x tt { i

TOTAL 12146 12450


82

LAKE NAME: LAY LAKE CONTINUED
STORET NUMBER: 0106

06 06 73 08 29 73 10 31 73

i ALGAL ALGAL I ALGAL ft

\ UNITS = | UNITS [ UNITS = {
TAXA FORM [S$ %C PER ML {S$ &C PER ML {S 2%C PER ML |
EUGLENA Cet fT (0.34 a TT ! x ff O61 20 4
FLAGELLATE #1 ceL { { 1 0.31 42 11 { |
FLAGELLATE #2 ceL ott 1 xX —~ 1 10l 127 Tt ! {
FLAGELLATES CEL «=43421e3f 1990 f51 Fe6f 1223 [2125.4] 910 |
FRANCETA cet fi 1 1 t 0.31 42 Vi | {
FRANCEIA CROESCFERI cel otf ! tt | tt { x 1
GCLENKINTA cee ott { ti { 1 { Os6t 20 «1
GYRO SIGMA cet ott { tt { x tt 1 1
KIRCHNERTELLA CCNTORTA ceu off 1 1 ! {1 o.st 20 1
LUNATE CELL cet of I Oe7! 62 Jt 163t 169 It ' x 1
LYNGBYA #1 FIL of I ! {ft tof 17 st ' {
LYNGBYA LIMNETICA FiL tt ! {| 2.64 337 TI ' |
MELCSIRA 2 cet =f ft O63 32 06 1 im) ! 1
MELOSIRA #2 CEL «11433021 3109 (1f 7631 928 «ft 2031 81 of
MELOSIRA #3 ceL ott { 1 { {5t 400f 142 |
MELOSIRA #4 CEL {51 363! 777 «| | 5.01 633 14 { x |
MELOSIRA CISTANS CEL 1411606) 1555 12131.81 4049 {3119.81 708 |
MELOSIRA GRANULATA cee ot iI ! x if { it { {
MERTSMOPEDIA MINIMA cou of ft (leo 93 (6d 1 tt i} {
MERISMOPEDIA TENUISSIMA cor of [ ' im { x 1 1 0.68 20 1
MY CRACTINIUM cor ft { x tf { i | |
NAVICULA CEL of I 0031 31 Ld 0031 42 tt | '
NAVICULA #1 cet if i} i \ x td 1 x 4
NE PHROCYTI UM cor ott | 1 f O03 42 tt { 1
NITZSCHIA ceu ott ! {1 t x tt ' x 1
NITZSCHTA 2 cel ot I oOe7t oz tft i ti i {
NITZSCHIA #1 cer oI I ! 11 0.71 84 1 | { I
CSCILLATORIA FIL I 1 x tt i] x [1 2.8! tor |
PEOIASTRUM BIRACIATUM 14 ! i { 't 1 {
Ve LONGEC ORNUTUP coo ot I 1 1 1 0031 42, 1 L O66l 20 |
FECTASTRUM BIRACIATUM ti { 11 ! tt 1 ]
Ve LONGECCRNUTUM ? cor of f 003! a1 6d ! ti i 1


LE

LAKE NAME: MITCHELL LAKE CONTINUED
STORET NUMBER: 0108

06 06 73 os 29 73 10 31 73
' ALGAL sf ALGAL I ALGAL
' UNITS I UNITS UNITS
TAXA FORM [S %C PER ML {S %C PER ML [S$ %C PER ML
SCENEDESMUS ACUMINATUS cou tt 1 ti tt f] x
SCENEDESMUS BISUGA cor ff { im) ! tt 1.4 38
SCENEDESMUS GRANULATUS tt 1 tt ! tt '
Fe CISCIFORMIS cor otf ! 11 0051 23, I I 064) 12
SCENEDESMUS QUADRICAUDA cor of { O.af 29° {41 be2] 295 dE «dE 203 63
SCHROEDERIA SETIGERA cer ott { {1 | 1 1 Qe4l 12
STEPHANODISCUS cee Ot i (sf 4.8! 228 {| | 3.7f LoL
SYNEORA cee (Ot tl 1 x tt I x tf ] x
TE TRAEDRON ceL oI ! x tf { {1 { x
TETRAECRON MINIMUM cer otf { 1 f o.st 23, | 0.41 12
TETRASTRUM cou ff (O68f 29 6 dt ! im {
TRACHELOMONAS, co ott 1 tt { tt { x
TREUBARTA CEL tt 1 1 1 0.51 23 It i}

TCTAL 3476 4791 2708


£2

LAKE NAME: HCLT LOCK AND DAM
STORET NUMBER: 0105

NYGAARD TROPHIC STATE INDICES
DATE 06 07 73 08 29 73 10 31 73

MYXCPHYCEAN o/o 0 6200 E 1.00 &
CHLOROPHYCE AN O1/O0 E 4200 E 4.00 E
EUGLENOPHYTE osol ? 0010 ? 0/05 2
DIATOM 0.60 E 0.80 E 0.33 E
COMPOUND 04/0 E 1560 & 6000 E

PALMER'S ORGANIC POLLUTICN INDICES
DATE 06 07 73 08 29 73: 10 31 73

GENUS oo o7 00
SPECIES 00 00 00

SPECIES DIVERSITY AND ABUNDANCE INDICES
DATE 06 07 73 08 29 73 10 31 73

AVERAGE DIVERSITY H 1.99 3283 1.81

NUMBER OF TAXA s 14200 30.00 11.00

NUMBER OF SAMPLES CCMPOSITED TM 4200 4.00 4.00
MAXIMUM DIVERSITY MAXH 3.81 4.91 3.46

TOTAL DIVERSITY o 3237073 5909069 280.55

TOTAL NUMBER OF INDIVIDUALS/ML N 1627¢00 1543200 155.00
EVENESS COMPONENT = J 0.52 0.78 0.52

MEAN NUMBER OF INDIVIDUALS/TAXA = L 116621 51243 14.09
NUMBER/ML OF MOST ABUNDANT TAXON K 664200 281.00 89200


In analyzing a water sample, any of the 20 genera or species of
algae present in concentrations of 50 per milliliter or more are
recorded. The pollution index numbers of the algae present are
totaled, providing a genus score and a species score. Palmer
determined that a score of 20 or more for either index can be taken as
evidence of high organic pollution, while a score of 15 to 19 is taken
as probable evidence of high organic pollution. Lower figures suggest
that the organic pollution of the sample is not high, that the sample
is not representative, or that some substance or factor interfering
with algal persistence is present and active.

SPECIES DIVERSITY AND ABUNDANCE INDICES

"Information content" of biological samples is being used
commonly by biologists as a measure of diversity. Diversity in this
connection means the degree of uncertainty attached to the specific
identity of any randomly selected individual. The greater the number
of taxa and the more equal their proportions, the greater the
uncertainty, and hence, the diversity (Pielou 1966). There are
several methods of measuring diversity, e.g., the formulas given by
Brillouin (1962) and Shannon and Weaver (1963). The method which is
appropriate depends on the type of biological sample on hand.

Pielou (1966) classifies the types of biological samples and
gives the measure of diversity appropriate for each type. The Survey
phytoplankton samples are what she classifies as larger samples
(collections in Pielou's terminology) from which random subsamples can
be drawn. According to Pielou, the average diversity per individual
for these types of samples can be estimated from the Shannon-Wiener
formula (Shannon and Weaver 1963):

Ss
H = Z, Ps log, P,

Where P is the proportion of the ith taxon in the sample, which is cal-
culated from n./N; n, is the number of individuals per milliliter of the
ith taxon; N id the total number of individuals per ml; and S is the total
number of taxa.

However, Basharin (1959) and Pielou (1966) have pointed out that
H calculated from the subsample is a biased estimator of the sample H,
and if this bias is to be accounted for, we must know the total num-
ber of taxa present in the sample since the magnitude of this bias
depends on it.

Pielou (1966) suggests that if the number of taxa in the
subsample falls only slightly short of the number in the larger
sample, no appreciable error will result in considering S, estimated


St

LAKE NAME: WILSCN LAKE
STORET NUMBER: 0114

NYGAARD TROPHIC STATE INDICES
DATE 06 09 73 08 16 73 10 22 73

MYXOPHYCE AN 01/0 E OB/O E 03/0 E
CHLORCPHYCEAN 02/0 E 16/0 E 03/0 E
EUGL ENOPHYTE 0/03 ? 0/24 2 0.17 2
DIATOM 1675 & 1.60 E 1.75 E
CCMPCUND 10/0 E 32/0 E 14/0 E

PALMER*S ORGANIC POLLUTION INDICES
DATE 06 09 73 08 16 73 10 22 73

GENUS. “02 13 c2
SPECIES 00 02 00

SPECIES DIVERSITY AND ABUNDANCE INDICES
DATE 06 09 73 O08 16 73 10 22 73

AVERAGE DIVERSITY H 1296 3036 2095

NUMBER OF TAXA $s 21.00 47200 25200

NUMBER OF SAMPLES COMPOSITED “ 3200 2.00 2200
WAXIMUM DIVERSITY MAXH 4039 5255 4064

TOTAL DIVERSITY oO 6569092 28882256 4873.40

TCTAL NUMBER OF INDIVIDUALS/ML N 3352200 8596.00 1652.00
EVENESS CCMPONENT J 0045 On61 0064

MEAN NUMBER OF INDIVIDUALS/TAXA Lt 159, €2 182.89 66.08
NUMBER/ML OF MOST ABUNDANT TAXON K 2076.00 3056200 4617200


lb

LAKE NAME: WILSON LAKE CONTINUED
STORET NUMBER: 0114

06 09 73 08 16 73 10 22 73
{ ALGAL | ALGAL Ot ALGAL
{ UNITS = { UNITS | UNITS

TAXA FORM |S %C PER ML {S %C PER ML 1S %C PER ML

MELOSIRA #3 cer oT 1 1 1 1 {1 2.41 39°
MELOSIRA #4 CEL «{1f6109f 2076 [1135-61 3056 [1128.31 467 |
MELOSIRA DISTANS cee ot t xX Tt Sell 461 FI 1 x f
MELOSIRA GRANULATA cer sot { 11 i5f 126 If ! !
MERTSMOPEDIA MARSSONIT co. ott | im 1 x Hl | 1
WERISMOPEDIA MINIMA cor of 4 t {1 5051 472 F1 ! !
MICROCYSTIS INCERTA cou it | tt ! x tt { x of
NITZSCHIA #1 cer tf { 14 | tt 1 x ot
NITZSCHIA #2 cer oft lel 47 tt ! tt { |
NITZSCHIA #3 cer ti { x [21 9091 850 1 1 ( !
NITZSCHIA #4 cer ot I ! ! t 0.41 31 6d 1 {
QSCILLATCRIA ANGUSTA Fir ! x {51 2.91 252 11 { x 1
PANDORINA MORUM cor of 1 xX 1 1 O64! 31 of | leat 19 4
PECTASTRUM SIMPLEX cou off l 11 ' x 1 1 I
PEDIASTRUM SIMPLEX Vt | tt ! tt { t
Ve CUODENARIUM cor ff I 4 t 11 1.28 1 I
PEANATE DIATOM #1 ceu ot | ti | x tt { x ot
PENNATE DIATOM #2 cer tt ! it { tt t x of
PEANATE OTATOM #3 ceu ott ! tt ! ti 1 x |
RAPHIDICPSIS Fi ot | ! It 0.71 63 (6 t I
SCENEDESMUS #1 cou 1) I 11 0041 31 6f | ileal |
SCENEDESMUS ABUNCANS co oft ' 1 ' x 4 | 1
SCENEDE SMUS ACUMINATUS cer ot t it 1 1d i x
SCENEDESMUS BIJUGA co ott { tt | x tt ! !
SCENEDESMUS DENTICULATUS cou oft i tt | x tt 1 i
SCENEDESMUS OPOLIENSTS co ott { t t x ti { i
SCENEDESMUS QUACRICAUDA im { tt ! tt ! I
Ve LONGISPINA coL {1 0.71 24 14 1 iat 1 1
SCEROEDERTA #1 cer ott ' tt ' x tt ' !
SPHAERELLCPS IS cer tt { im! { x tt ‘ ‘
STEPHANCOI SCUS ceu ot I el pe Oe eed ee se
SYNEDRA #1 ce ot I i x Ut | x 4 ' |


LITERATURE CITED

Basharin, G. P. 1959. Ona statistical estimate for the entrophy of a
sequence of independent random variables, pp. 333-336. InN.
Artin (ed.), Theory of Probability and Its Applications
(translation of "Teoriya Veroyatnosei i ee Premeneniya") 4.
Society for Industrial and Applied Mathematics, Philadelphia.

Brillouin, L. 1962. Science and Information Theory (2nd ed.).
Academic Press, New York. 351 pp.

Hutchinson, G. E. 1967. A Treatise on Limnology. II. Introduction
to Lake Biology and the Limnoplankton. John Wiley and Sons,

Inc., New York. 1,115 pp.

Nygaard, G. 1949. Hydrobiological studies of some Danish ponds and
Jakes. II. (K danske Vidensk. Selsk.) Biol. Sci. 7:293,

Palmer, C. M. 1969. A composite rating of algae tolerating organic
pollution. J. Phycol. 5:78-82.

Pielou, E. C. 1966. The measurement of diversity in different types
of biological collections. J. Theor. Biol. 13:131-144.

Prescott, G. W. 1970. How to Know the Freshwater Algae. William C.
Brown Company, Dubuque. 348 pp.

Sager, P. E. and A. D. Hasler. 1969. Species diversity in laucustrine
phytoplankton. I. The components of the index of diversity from
Shannon's formula. Amer. Natur. 103(929):51-59.

Shannon, C. E. and W. Weaver. 1963. The Mathematical Theory of
Communication. University of Illinois Press, Urbana. 117 pp.

U.S. Environmental Protection Agency. 1975. National Eutrophication
Survey Methods 1973-1976. Working Paper No. 175. Environmental
Monitoring and Support Laboratory. Las Vegas, Nevada, and
Corvallis Environmental Research Laboratory, Corvallis, Oregon.

91 pp.

Zand, S. M. 1976. Indexes associated with information theory in water
quality. Journal WPCF. 48(8):2026-2031.

10