### UNITED STATES ENVIRONMENTAL PROTECTION AGENCY WASHINGTON D.C., 20460

OFFICE OF PREVENTION, PESTICIDES AND TOXIC SUBSTANCES



### PC Code: 129112 DP Barcode: 361159

Date: June 26, 2009

MEMORANDUM

SUBJECT: Trifloxystrobin Ecological Risk Re view for a Rate Increase and Formulation Change for Use on Soybeans

FROM: Michael Lowit, Ecologist Mark Corbin, Senior Environmental Fate Scientist Environmental Risk Branch III Environmental Fate and Effects Division (7507P)

TO: Tony Kish Registration Division (RM 22)

THRU: Dana Spatz Branch Chief, Environmental Risk Branch III Environmental Fate and Effects Division (7507P)

This memorandum summarizes the ecological risks associated with the proposed new use of trifloxystrobin as a fungicide on soybeans. The risk assessment considers the product Stratego ® 

### 731 Fungicide (EPA Reg. No. 264-RNOG).

Risk Summary

The Environmental Fate and Effects Division (EFED) has evaluated the new use request for a formulation change and rate increase for the use of trifloxystrobin on soybeans as a foliar spray (aerial and ground) and by chemigation. This risk assessment considers Stratego 731 ® 

### Fungicide (EPA Reg. No. 264-RNOG) which contains bot h trifloxystrobin and prothioconazole. Prothioconazole was not considered in this assessment, and potential interactions (additive, synergistic, or inhibitory) between the two active ingredients were not considered.

No new environmental fate or effects data have been submitted since the most recent ecological risk assessment for soybeans was conducted on December 19, 2005 (D318621, D318623). The

### 2 current assessment relies on the data summaries and risk conclusions from the previous assessment. In order to avoid repetition of material previously provided, please refer to the previous assessment (D318621, D318623) for detailed information.

The current request is for EFED to consider the impact of a formulation change from an emulsifiable concentrate (EC) evaluated previous ly to a soluble concentrate (SC) of the new product. Because both formulations will behave essentially the same once applied in the field, the data and summaries from the previous assessment cover this new use.

In addition, the new request is for a rate increase to 0.114 lbs ai/acre applied three times per year with 10 day intervals between applications which is higher than the previously assessed rate of 0.082 lbs ai/acre with three applications per year and 10 day intervals. EFED’s assessment of the new use rate indicates that no taxa exceed the Agency’s LOC similar to the conclusion from the 2005 risk assessment for soybean use (D318621, D318623). However, previous assessments of other uses of trifloxystrobin have shown exceedences of the Agency’s LOC.

Trifloxystrobin has been previously evaluated by EFED for use on numerous commodities including pome fruits, grapes, cucurbits, peanuts, and turf (New Chemical Review, D244219, D245281, D254206, D254285, D254286); almonds, fruiting vegetables, hops, potatoes, sugar beets, and wheat (D254215, D254220, D254222); root vegetable subgroup 1B (except beet and radish) and leaf petiole subgroup 4B (D286427); barley, oats, sweet corn, and soybeans (D315580, D314187, D318621, D318623); grass grown for seed (D332979, D332982); asparagus, tropical crops, radis h, root vegetables, and strawberries (D331915, D339548, D348876). Detailed summaries of these assessments can be found elsewhere (D309489, D331915). The proposed application rates for the new use on soybeans is less than several of the rates (turf, cucurbits, grapes, grass grown for seed, asparagus, tropical crops, radish, root vegetables, leaf petiole, almonds, fruiting vegetables, hops, and potatoes) previously considered.

Based on previous reviews, EFED concluded that some uses are expected to present risk on an acute basis to non-target aquatic organisms (fish and invertebrates); including Federally-listed endangered and threatened species. Those uses include turf, cucurbits, grasses grown for seed, tropical crops, root vegetables, leaf petiole, almonds, fruiting vegetables, hops, and potatoes.

The key uncertainties identified in the 2005 risk assessment (D318621, D318623) are applicable to the current assessment:

• Risk to endangered terrestrial plants could not be assessed due to the limitations of the submitted Tier I toxicity studies. Therefore, risk is presumed because suitable Tier II plant toxicity studies are not available for consideration.

• In addition, another strobilurin fungicide is highly toxic to some orchard fruits, including apples. Data on trifloxystrobin toxicity to apples or other orchard fruits were not submitted. Therefore, some risk to apples and other orchard fruits is presumed.

### 3 • A chronic estuarine/marine invertebrate toxicity study has not been submitted. The mysid shrimp NOAEC of 0.96 ppb was estimated using an acute to chronic ratio derived from daphnid studies. If the acute to chronic ratio is not conserved across species, then risk could be over- or under-estimated.

• A suitable aerobic aquatic metabolism study (162-4) has not been submitted; therefore, there remains a degree of uncertainty as to the persistence and dissipation of trifloxystrobin in aquatic environments.

• Stratego 731 ® 

### Fungicide contains both trifloxystrobin and prothioconazole. Potential interactions (additive, synergistic, or inhibitory) between the two active ingredients could result in an under- or over-estimation of the risk based on trifloxystrobin alone.

Analysis

Because the rates for the new use are higher than those previously assessed for soybeans and because EFED’s aquatic and terrestrial exposure models have been updated since the 2005 assessment an evaluation of the impact of the rate change on exposure has been completed. These revised EECs were then compared to the toxicity data presented in the 2005 assessment (D318621, D318623) (Attachment 1). The previously submitted data were not re-evaluated. Table 1 summarizes the previous aquatic EECs relative to the new EECs.

Table 1 Summary of Previous and Current Surface Water EECs 1, 2



### Scenario Use Application Rate/ # Applications/ Interval Peak (ug/L) 21 day (ug/L) 60 day (ug/L)

Previous Assessment MS soybean Soybean 0.082 lbs ai/A 3 applications 10 days 0.28 0.07 0.03

Current Assessment MS soybean Soybean 0.114 lb ai/A 3 applications 10 days 0.36 0.15 0.09

1 

### The previously summarized environmental fate data and PRZM model input/output for the current EECs are included in Attachments 2 and 3, respectively.

2 

### Surface water EECs were modeled only for trifloxystrobin.

The surface water EECs are based on aerial application and are protective of foliar ground applications. The groundwater EEC (0.00007 ug/L) for the proposed use is much lower than the surface water EECs (Table 1), thus the estimat ed surface water EECs are also protective of chemigation applications. SCI-GROW (v2.3) was used to estimate the groundwater concentration using input values summarized in Attachment 2.

### 4 Comparison of these updated aquatic EECs with the previously summarized toxicity data indicate that none of the Agency’s levels of concern for aquatic species is exceeded. The revised aquatic risk quotients for the increased rate are summarized in Attachment 4.

Similarly, EFED has evaluated the impact of the rate increase on terrestrial exposure. The rate is approximately 40% higher than the previously assessed rate and inputting the new rate in T-REX (v1.4.1) yields terrestrial EECs that are higher by a similar magnitude.

Tables 2 and 3 summarize the terrestrial EECs for the proposed new use. Input values for exposure estimates obtained from T-REX simulations of trifloxystrobin are as follows: application rate = 0.114 lb ai/A, number of applications = 3, application interval = 10 days, default foliar dissipation half-life = 35 days (EFED guidance).

Table 2 Dietary-based Terrestrial Food Item Residue Estimates for Proposed Use Food Item Avian and Mammal Dietary-based EECs (ppm) Short Grass 68.22 Tall Grass 31.27 Broadleaf plants/sm Insects 38.37 Fruits/pods/seeds/lg insects 4.26

Table 3 Dose-based Terrestrial Food Item Residue Estimates for Proposed Use Avian Classes and Body Weights (grams) Mammal Classes and Body Weights (grams)

small mid large small mid large Food Item

20 100 1000 15 35 1000 Short Grass 77.69 1 

### 44.30 19.84 65.04 44.95 10.42 Tall Grass 35.61 20.31 9.09 29.81 20.60 4.78 Broadleaf plants/sm Insects 43.70 24.92 11.16 36.58 25.28 5.86

Fruits/pods/seeds/lg insects 4.86 2.77 1.24 4.06 2.81 0.65

1 

### Dose-based EEC (mg/kg-bw)

Comparison with toxicity data summarized in the previous assessment indicates that although predicted terrestrial EECs will be roughly 40% higher than those in the previous assessment, no exceedances of the Agency’s LOC for birds and mammals are predicted. A summary of the revised terrestrial RQs for the increased rate are included in Attachment 5. Typically, acute terrestrial animal RQs are not calculated when definitive toxicity endpoints are not available as is the case for trifloxystrobin (i.e., avian LD 50 

### > 2000 mg/kg-bw; avian LC 50 

### > 5050 mg/kg-feed, mammal LD 50 

### > 5000 mg/kg-bw). Nonetheless, if acute RQs were calculated using these data, all avian and mammal acute RQs would be less than the Agency’s LOC.

Finally, trifloxystrobin did not cause >25% inhibition in tier I terrestrial plant toxicity studies at 0.249 lbs ai/Acre, which is above the maximum proposed application rate being assessed in this evaluation (0.114 lbs ai/Acre). Therefore, Tier II studies are not required. However, because

### 5 inhibition was observed, and the data from a Tier I study are not sufficient to allow for calculation of a slope, an EC 05 

### , or a NOAEC, risk to endangered plant species cannot be assessed and, therefore, cannot be precluded.

### 6 Attachment 1

Ecological Effects Characterization

A summary of trifloxystrobin toxicity data is pres ented below. A full discussion of the available data is included in the New Chemical Review (D254286) and new use reviews (D254215, -20, - 22, and D309489). No additional toxicity data have been submitted since the last assessment was completed in 2008 (D331915). Previously submitted data were not re-evaluated. Overall, trifloxystrobin is practically non-toxic to mammals and birds on an acute oral basis. Trifloxystrobin was not a reproductive toxicant in mammals or birds under the conditions of the submitted studies. In contrast, trifloxystrobin is highl y toxic to fish and aquatic invertebrates. A summary of the key toxicity values that are used in this assessment is reported in Tables A and B below.

Because the parent compound degrades rapidly to its major degradate, CGA-321113, which is relatively stable, chronic exposure in most aquatic environments is expected to occur mainly from this degradate. CGA-321113 has been studied in fish and aquatic invertebrates in acute studies and is practically non-toxic to these surrogate species on an acute basis. The degradate is also considerably less toxic than trifloxystrobin to daphnids on a chronic basis. Therefore, it is anticipated that risk quotients based on chronic studies using trifloxystrobin would be protective of aquatic organisms exposed to CGA-321113 on a chronic basis. The available data do not allow for a comparison of the relative toxicities of trifloxystrobin and the major degradate in terrestrial organisms.

Table A. Terrestrial Toxicity Profile for Trifloxystrobin

Test Type Value Used in Risk Assessment Comments MRID

Rat acute oral LD50 >5000 mg/kg-bw Acceptable study. 44496622

Rat 2-generation reproduction NOAEL 1500 mg/kg-feed Acceptable study. No treatment-related effects were observed at any concentration tested (up to 1500 ppm feed). 44496710

Bird acute oral LD50 (Mallard Duck, Bobwhite Quail) LD50: >2000 - >2250 mg/kg-bw Acceptable studies. No mortality or treatmentrelated toxic effects occurred in either species. 44496606 44496605

Bird dietary LC50 (Mallard duck and Bobwhite quail) >5050 mg/kg-feed Acceptable study. No mortality or treatmentrelated toxic effects occurred in either species. 44496607 44496608

Reproductive Toxicity NOAEL (Bobwhite) 320 mg/kg-feed Acceptable study. No treatment-related effects were observed at any concentration tested (up to 320 ppm feed). 44496609

Reproductive Toxicity NOAEL (Mallard duck) 500 mg/kg-feed Acceptable study. No treatment-related effects were observed at any concentration tested (up 44496610

### 7 Test Type Value Used in Risk Assessment Comments MRID

to 500 ppm feed).

Honey Bee Contact LD50 >200 ug/bee Acceptable study. No mortality was observed. 44496726

Terrestrial Plants (Tier I) None used. EC25 is greater than the highest concentration tested (0.25 lbs a.i./Acre) Supplemental Study. The study is acceptable; however, it does not allow for an assessment of risk to endangered species because a 13% effect was observed in cabbage. The data do not allow for derivation of a NOAEC or an EC05. 44496723



### Table B. Aquatic Toxicity Profile for Trifloxystrobin

Test Type Value Used in Risk Assessment Comments MRID

Rainbow Trout LC50 Degradate CGA-321113

Bluegill Sunfish LC50 14 ppb >106,000 ppb

54 ppb Acceptable study Supplemental study (insufficient number of test organisms) Acceptable study 44502803 44527502

44502802

Daphnia magna EC50 Degradate CGA-321113 25 ppb >95,000 ppb Acceptable studies Acceptable studies 44496611 44527503

Sheepshead minnow LC50 78 ppb Acceptable study 44496612

Oyster shell deposition EC50 29 ppb Acceptable study 44496613

Mysid shrimp LC50 8.6 ppb Acceptable study 44496614

Mysid shrimp NOAEC 0.96 ppb No study submitted. The estuarine/marine chronic NOAEC was estimated using acute to chronic ratios (ACRs) derived from daphnid acute (EC50=25 ppb) and chronic (NOAEC = 2.8 ppb) toxicity values. The ACR was 8.9. Therefore, the estimated NOAEC in mysid shrimp would be 8.6/8.9 = 0.96 ppb. Not applicable

Rainbow Trout Early Life Stage NOAEC: 4.3 ppb Acceptable study. The LOAEC was 7.7 ppb based on reduced survival. 44502804

Daphnia magna life cycle

Degradate CGA-321113 NOAEC: 2.8 ppb

NOAEC: 3,200 ppb Acceptable study. The LOAEC was 5.9 ppb based on reduced survival and reproduction.

Supplemental study. Water parameter measurements and growth endpoints of weight and length were not measured 44496615

44869701

Green algae

Degradate CGA-321113 EC50: 37 ppb

EC50: 78,800 ppb Acceptable study. The NOAEC was 10 ppb.

Supplemental study. Test duration was only 72 hours; a definitive EC50 was not determined 44496724

44527504

### 8 Test Type Value Used in Risk Assessment Comments MRID

Duckweed ( Lemna gibba ) EC50: >1930 ppb Acceptable study. The NOAEC was 410 ppb. 44496725

### 9 Attachment 2

Summary of Environmental Fate Data and Model Input Parameters for Trifloxystrobin

Parameter Value Modeling Input Value Source

Molecular Formula C 20 H 19 F 3 N 2 O 4 DP Barcode D254920

Molecular Weight 408.4 408.4 DP Barcode D254920

Solubility @ 20 ° C 0.61 mg/L @ pH7 0.61 mg/L DP Barcode D254920

Vapor Pressure 3.14e -06 Pa @ 25 ° C 2.55e -08 mm Hg @ 20 ° C DP Barcode D254920

Hydrolysis Half-life 55.2 days @ pH5 0.83 days @ pH 7,9 55.2 days 0.83 days MRID 44496727

Aquatic Photolysis Halflife 14.8 days @pH 5 14.8 days MRID 44496727

Photolysis on Soil Halflife 2.1 days 2.1 days MRID 44496729

Aerobic Soil Metabolism Half-life (T 1⁄2 ) 0.74, 0.41, and 0.91 days

mean = 0.69 d, std = 0.25 d 90 th percent upper bound of mean = 0.96 days (PRZM/EXAMS and SCI-GROW) MRID 44496732 MRID 44496730 MRID 44496731 EFED Guidance

Anaerobic Aquatic Metabolism Half-life 0.72 days 2.16 days (3 x 0.72) MRID 44496733

Aerobic Aquatic Metabolism Half-life 0.60 days (supplemental) 2 x aerobic soil metabolism (0.96 days) 1.92 MRID 44496734 EFED Guidance

Organic Carbon Partition Coefficient (Koc) (mL/g) 2354, 951, 3064, 3927, 9871, 951

r 2 = (Kads vs org. matter) = 0.03) Use mean Kads (PRZM/EXAMS)lowest Koc (SCIGROW) MRID 44496802

Soil Partition Coefficient (Kads) (mL/g) (Kads) 6.8, 7.7, 16.0, 59.4, 91.8, 33.2 Mean = 35.82; Std = 33.8 7.7 lowest non-sand

mean Kads = 35.8 1 MRID 44496802

Aerial Spray Drift Application Efficiency 0.05 0.95 previous column EFED Input

FEXTRA - foliar apps PRZM Foliar Extraction 0.5 (frac/cm rain) EFED Guidance

PLDKRT - foliar apps PRZM Decay rate of Foliage 35 days EFED Guidance

1 The calculated mean Kads value of 26.9 mL/g used in the previous assessment of soybeans (D318621, D318623) was calculated incorrectly (the 59.4 value was incorrectly entered as 5.94). The correct mean value is 35.82 mL/g as indicated in the most recent new use assessment for trifloxystrobin (D331915).

### 10 ATTACHMENT 3

PRZM/EXAMS Input & Output

MS soybean; 8/9/01 "Yazoo County; MLRA 134; Metfile: W03940.dvf (old: Met131.met)," *** Record 3: 0.75 0.36 0 25 1 3 *** Record 6 -- ERFLAG 4 *** Record 7: 0.42 0.3 1 10 3 2 356.8 *** Record 8 1 *** Record 9 1 0.2 30 100 3 87 84 87 0 76 *** Record 9a-e 1 27 1604 2004 0105 0505 1605 0106 1606 0107 1607 0108 1608 0109 1609 0110 1510 1610 .498 .575 .627 .654 .620 .484 .361 .220 .094 .109 .110 .046 .053 .040 .203 .239 .014 .014 .014 .014 .014 .014 .014 .014 .014 .014 .014 .014 .014 .014 .014 .014 84 84 84 84 84 84 84 84 84 84 84 84 84 84 87 87 0111 1611 0112 1612 0101 1601 0102 1602 0103 1603 0104 .316 .394 .464 .524 .245 .276 .306 .337 .373 .418 .468 .014 .014 .014 .014 .014 .014 .014 .014 .014 .014 .014 87 87 87 87 87 87 87 87 87 87 87 *** Record 10 -- NCPDS, the number of cropping periods 30 *** Record 11 160461 010961 101061 1 160462 010962 101062 1 160463 010963 101063 1 160464 010964 101064 1 160465 010965 101065 1 160466 010966 101066 1 160467 010967 101067 1 160468 010968 101068 1 160469 010969 101069 1 160470 010970 101070 1 160471 010971 101071 1 160472 010972 101072 1 160473 010973 101073 1 160474 010974 101074 1 160475 010975 101075 1 160476 010976 101076 1 160477 010977 101077 1 160478 010978 101078 1 160479 010979 101079 1 160480 010980 101080 1

### 11 160481 010981 101081 1 160482 010982 101082 1 160483 010983 101083 1 160484 010984 101084 1 160485 010985 101085 1 160486 010986 101086 1 160487 010987 101087 1 160488 010988 101088 1 160489 010989 101089 1 160490 010990 101090 1 *** Record 12 -- PTITLE trifloxystrobin - 3 applications @ 0.128 0.128 0.128 kg/ha *** Record 13 90 1 0 0 *** Record 15 -- PSTNAM trifloxystrobin *** Record 16 011061 0 2 4.0 0.128 .95 .05 111061 0 2 4.0 0.128 .95 .05 011161 0 2 4.0 0.128 .95 .05 011062 0 2 4.0 0.128 .95 .05 111062 0 2 4.0 0.128 .95 .05 011162 0 2 4.0 0.128 .95 .05 011063 0 2 4.0 0.128 .95 .05 111063 0 2 4.0 0.128 .95 .05 011163 0 2 4.0 0.128 .95 .05 011064 0 2 4.0 0.128 .95 .05 111064 0 2 4.0 0.128 .95 .05 011164 0 2 4.0 0.128 .95 .05 011065 0 2 4.0 0.128 .95 .05 111065 0 2 4.0 0.128 .95 .05 011165 0 2 4.0 0.128 .95 .05 011066 0 2 4.0 0.128 .95 .05 111066 0 2 4.0 0.128 .95 .05 011166 0 2 4.0 0.128 .95 .05 011067 0 2 4.0 0.128 .95 .05 111067 0 2 4.0 0.128 .95 .05 011167 0 2 4.0 0.128 .95 .05 011068 0 2 4.0 0.128 .95 .05 111068 0 2 4.0 0.128 .95 .05 011168 0 2 4.0 0.128 .95 .05 011069 0 2 4.0 0.128 .95 .05 111069 0 2 4.0 0.128 .95 .05 011169 0 2 4.0 0.128 .95 .05 011070 0 2 4.0 0.128 .95 .05 111070 0 2 4.0 0.128 .95 .05 011170 0 2 4.0 0.128 .95 .05 011071 0 2 4.0 0.128 .95 .05 111071 0 2 4.0 0.128 .95 .05 011171 0 2 4.0 0.128 .95 .05

### 12 011072 0 2 4.0 0.128 .95 .05 111072 0 2 4.0 0.128 .95 .05 011172 0 2 4.0 0.128 .95 .05 011073 0 2 4.0 0.128 .95 .05 111073 0 2 4.0 0.128 .95 .05 011173 0 2 4.0 0.128 .95 .05 011074 0 2 4.0 0.128 .95 .05 111074 0 2 4.0 0.128 .95 .05 011174 0 2 4.0 0.128 .95 .05 011075 0 2 4.0 0.128 .95 .05 111075 0 2 4.0 0.128 .95 .05 011175 0 2 4.0 0.128 .95 .05 011076 0 2 4.0 0.128 .95 .05 111076 0 2 4.0 0.128 .95 .05 011176 0 2 4.0 0.128 .95 .05 011077 0 2 4.0 0.128 .95 .05 111077 0 2 4.0 0.128 .95 .05 011177 0 2 4.0 0.128 .95 .05 011078 0 2 4.0 0.128 .95 .05 111078 0 2 4.0 0.128 .95 .05 011178 0 2 4.0 0.128 .95 .05 011079 0 2 4.0 0.128 .95 .05 111079 0 2 4.0 0.128 .95 .05 011179 0 2 4.0 0.128 .95 .05 011080 0 2 4.0 0.128 .95 .05 111080 0 2 4.0 0.128 .95 .05 011180 0 2 4.0 0.128 .95 .05 011081 0 2 4.0 0.128 .95 .05 111081 0 2 4.0 0.128 .95 .05 011181 0 2 4.0 0.128 .95 .05 011082 0 2 4.0 0.128 .95 .05 111082 0 2 4.0 0.128 .95 .05 011182 0 2 4.0 0.128 .95 .05 011083 0 2 4.0 0.128 .95 .05 111083 0 2 4.0 0.128 .95 .05 011183 0 2 4.0 0.128 .95 .05 011084 0 2 4.0 0.128 .95 .05 111084 0 2 4.0 0.128 .95 .05 011184 0 2 4.0 0.128 .95 .05 011085 0 2 4.0 0.128 .95 .05 111085 0 2 4.0 0.128 .95 .05 011185 0 2 4.0 0.128 .95 .05 011086 0 2 4.0 0.128 .95 .05 111086 0 2 4.0 0.128 .95 .05 011186 0 2 4.0 0.128 .95 .05 011087 0 2 4.0 0.128 .95 .05 111087 0 2 4.0 0.128 .95 .05 011187 0 2 4.0 0.128 .95 .05 011088 0 2 4.0 0.128 .95 .05 111088 0 2 4.0 0.128 .95 .05

### 13 011188 0 2 4.0 0.128 .95 .05 011089 0 2 4.0 0.128 .95 .05 111089 0 2 4.0 0.128 .95 .05 011189 0 2 4.0 0.128 .95 .05 011090 0 2 4.0 0.128 .95 .05 111090 0 2 4.0 0.128 .95 .05 011190 0 2 4.0 0.128 .95 .05 *** Record 17 0 1 0 *** Record 18 0 35 0.5 *** Record 19 -- STITLE Loring Silt Loam; HYDG: C *** Record 20 155 0 0 0 0 0 0 0 0 0 *** Record 26 0 0 0 *** Record 33 6 1 13 1.4 0.385 0 0 0 0.7220280.722028 0 0.1 0.385 0.151 1.28 35.8 2 23 1.4 0.37 0 0 0 0.7220280.722028 0 1 0.37 0.146 0.49 35.8 3 33 1.4 0.37 0 0 0 0.7220280.722028 0 3 0.37 0.146 0.16 35.8 4 30 1.45 0.34 0 0 0 0.7220280.722028 0 5 0.34 0.125 0.12 35.8 5 23 1.49 0.335 0 0 0 0.7220280.722028 0 1 0.335 0.137 0.07 35.8 6 33 1.51 0.343 0 0 0 0.7220280.722028 0 3 0.343 0.147 0.06 35.8 ***Record 40 0 YEAR 10 YEAR 10 YEAR 10 1 1 1 ----- 7 YEAR PRCP TCUM 0 0 RUNF TCUM 0 0 INFL TCUM 1 1 ESLS TCUM 0 0 1.0E3 RFLX TCUM 0 0 1.0E5 EFLX TCUM 0 0 1.0E5 RZFX TCUM 0 0 1.0E5

### 14 stored as trifloxystrobin Eco Rev.out Chemical: trifloxystrobin PRZM environment: MSsoybeanSTD.txt modified Tueday, 29 May 2007 at 12:58:06 EXAMS environment: pond298.exv modified Thuday, 29 August 2002 at 16:33:29 Metfile: w03940.dvf modified Wedday, 3 July 2002 at 10:05:46 Water segment concentrations (ppb)

Year Peak 96 hr 21 Day 60 Day 90 Day Yearly 1961 0.351 0.2401 0.1259 0.07877 0.05274 0.01301 1962 0.3413 0.2379 0.1104 0.07319 0.04911 0.01211 1963 0.3365 0.2291 0.1019 0.06605 0.04423 0.01091 1964 0.3567 0.2396 0.1342 0.07717 0.05155 0.01268 1965 0.3568 0.2399 0.1344 0.07637 0.051 0.01258 1966 0.3613 0.2474 0.1407 0.08331 0.05575 0.01375 1967 0.3586 0.2487 0.1372 0.08651 0.05803 0.01431 1968 0.3464 0.2402 0.1188 0.07749 0.05202 0.01279 1969 0.3461 0.2405 0.1183 0.07747 0.05201 0.01283 1970 0.4071 0.3023 0.1501 0.08946 0.05992 0.01478 1971 0.3389 0.2346 0.1062 0.07017 0.04696 0.01158 1972 0.3427 0.2406 0.1128 0.0752 0.05046 0.01241 1973 0.3415 0.2184 0.1109 0.06569 0.04385 0.01081 1974 0.3526 0.2341 0.1286 0.07839 0.05246 0.01293 1975 0.3475 0.2333 0.1244 0.07668 0.05131 0.01265 1976 0.3651 0.2547 0.146 0.09565 0.06458 0.01588 1977 0.3557 0.2398 0.1328 0.08 0.05349 0.0132 1978 0.355 0.2367 0.1318 0.0753 0.05029 0.0124 1979 0.351 0.2459 0.1258 0.0832 0.05599 0.01381 1980 0.3583 0.2425 0.1366 0.0852 0.05717 0.01406 1981 0.3479 0.2269 0.1211 0.07257 0.04851 0.01197 1982 0.3495 0.2308 0.1235 0.07506 0.05019 0.01238 1983 0.3471 0.2326 0.1199 0.07441 0.04985 0.01229 1984 0.3359 0.2414 0.1009 0.06961 0.04661 0.01147 1985 0.3426 0.2148 0.1127 0.06519 0.04352 0.01073 1986 0.3499 0.2589 0.1333 0.07659 0.05116 0.01261 1987 0.3635 0.2513 0.1437 0.08394 0.05612 0.01384 1988 0.5296 0.3592 0.1846 0.09573 0.06392 0.01572 1989 0.3532 0.2334 0.1292 0.07727 0.05171 0.01275 1990 0.3538 0.2346 0.1301 0.07725 0.05164 0.01274

Sorted results Prob. Peak 96 hr 21 Day 60 Day 90 Day Yearly 0.032258064516129 0.5296 0.3592 0.1846 0.09573 0.06458 0.01588 0.0645161290322581 0.4071 0.3023 0.1501 0.09565 0.06392 0.01572 0.0967741935483871 0.3651 0.2589 0.146 0.08946 0.05992 0.01478 0.129032258064516 0.3635 0.2547 0.1437 0.08651 0.05803 0.01431 0.161290322580645 0.3613 0.2513 0.1407 0.0852 0.05717 0.01406 0.193548387096774 0.3586 0.2487 0.1372 0.08394 0.05612 0.01384 0.225806451612903 0.3583 0.2474 0.1366 0.08331 0.05599 0.01381

### 15 0.258064516129032 0.3568 0.2459 0.1344 0.0832 0.05575 0.01375 0.290322580645161 0.3567 0.2425 0.1342 0.08 0.05349 0.0132 0.32258064516129 0.3557 0.2414 0.1333 0.07877 0.05274 0.01301 0.354838709677419 0.355 0.2406 0.1328 0.07839 0.05246 0.01293 0.387096774193548 0.3538 0.2405 0.1318 0.07749 0.05202 0.01283 0.419354838709677 0.3532 0.2402 0.1301 0.07747 0.05201 0.01279 0.451612903225806 0.3526 0.2401 0.1292 0.07727 0.05171 0.01275 0.483870967741936 0.351 0.2399 0.1286 0.07725 0.05164 0.01274 0.516129032258065 0.351 0.2398 0.1259 0.07717 0.05155 0.01268 0.548387096774194 0.3499 0.2396 0.1258 0.07668 0.05131 0.01265 0.580645161290323 0.3495 0.2379 0.1244 0.07659 0.05116 0.01261 0.612903225806452 0.3479 0.2367 0.1235 0.07637 0.051 0.01258 0.645161290322581 0.3475 0.2346 0.1211 0.0753 0.05046 0.01241 0.67741935483871 0.3471 0.2346 0.1199 0.0752 0.05029 0.0124 0.709677419354839 0.3464 0.2341 0.1188 0.07506 0.05019 0.01238 0.741935483870968 0.3461 0.2334 0.1183 0.07441 0.04985 0.01229 0.774193548387097 0.3427 0.2333 0.1128 0.07319 0.04911 0.01211 0.806451612903226 0.3426 0.2326 0.1127 0.07257 0.04851 0.01197 0.838709677419355 0.3415 0.2308 0.1109 0.07017 0.04696 0.01158 0.870967741935484 0.3413 0.2291 0.1104 0.06961 0.04661 0.01147 0.903225806451613 0.3389 0.2269 0.1062 0.06605 0.04423 0.01091 0.935483870967742 0.3365 0.2184 0.1019 0.06569 0.04385 0.01081 0.967741935483871 0.3359 0.2148 0.1009 0.06519 0.04352 0.01073

0.1 0.36494 0.25848 0.14577 0.089165 0.059731 0.014733 Average of yearly averages: 0.012866

Inputs generated by pe 5.pl - Novemeber 2006

Data used for this run: Output File: trifloxystrobin Eco Rev Metfile: w03940.dvf PRZM scenario: MSsoybeanSTD.txt EXAMS environment file: pond298.exv Chemical Name: trifloxystrobin Description Variable Name Value Units Comments Molecular weight mwt 408.4 g/mol Henry's Law Const. henry 2.25E-8 atm-m^3/mol Vapor Pressure vapr torr Solubility sol 61 mg/L Kd Kd 35.8 mg/L Koc Koc mg/L Photolysis half-life kdp 14.8 days Half-life Aerobic Aquatic Metabolism kbacw 1.92 days Halfife Anaerobic Aquatic Metabolism kbacs 2.16 days Halfife Aerobic Soil Metabolism asm 0.96 days Halfife Hydrolysis: pH 7 0 days Half-life Method: CAM 2 integer See PRZM manual Incorporation Depth: DEPI 4 cm Application Rate: TAPP 0.128 kg/ha

### 16 Application Efficiency: APPEFF 0.95 fraction Spray Drift DRFT 0.05 fraction of application rate applied to pond Application Date Date 01-10 dd/mm or dd/mmm or dd-mm or dd-mmm Interval 1 interval 10 days Set to 0 or delete line for single app. app. rate 1 apprate kg/ha Interval 2 interval 21 days Set to 0 or delete line for single app. app. rate 2 apprate kg/ha Record 17: FILTRA IPSCND 1 UPTKF Record 18: PLVKRT PLDKRT 35 FEXTRC 0.5 Flag for Index Res. Run IR EPA Pond Flag for runoff calc. RUNOFF none none, monthly or total(average of entire run)

### 17 Attachment 4

Aquatic RQs

Effect Endpoint (ug/L) EEC (ug/L) RQ Acute Freshwater Fish 96-hr LC 50 

### :14 a 

### Peak: 0.36 0.026 Chronic Freshwater Fish NOAEC: 4.3 b 

### 60-day: 0.09 0.021

Acute Freshwater Invertebrates 48-hr EC 50 

### : 25 c 

### Peak: 0.36 0.014

Chronic Freshwater Invertebrates NOAEC: 2.8 d 

### 21-day: 0.15 0.054

Acute Marine/Estuarine Fish LC 50 

### : 78 e 

### Peak: 0.36 0.005

Acute Marine/Estuarine Invertebrates 96-hr LC 50 

### : 8.6 f 

### Peak: 0.36 0.042

Chronic Marine/Estuarine Invertebrates NOAEC: 0.96 g 

### 21-day: 0.15 0.156

Non-vascular aquatic plants 96-hr EC 50 

### : 37 h



### NOAEC: 10 Peak: 0.36 Peak: 0.36 0.010 0.036

a 96-hr LC 50 in rainbow trout (MRID 44502803)

b Early life stage toxicity study in rainbow trout (MRID 44502804)

c 48-hr EC 50 in Daphnia magna (MRID 44496611)

d 21-day Daphnia magna study (MRID 44496615)

e LC 50 in Sheepshead minnow (MRID 44496612)

f 96-hr LC 50 in mysid shrimp (MRID 44496614)

g The estuarine/marine chronic NOAEC was estimated us ing acute to chronic ratios (ACRs) derived from daphnid acute (EC50=25 ppb) and chronic (NOAEC = 2.8 ppb) toxicity values. The ACR was 8.9. Therefore, the estimated NOAEC in mysid shrimp would be 8.6/8.9 = 0.96 ppb.

h 96-hr EC50 in algae (MRID 44496724); the EC50 and NOAE C in duckweed were considerably higher (MRID 44496725). Therefore, risk to vascular pl ants is presumably lower than risk to non-vascular plants such as algae.

### 18 Attachment 5

Terrestrial RQs

Upper Bound Kenaga, Acute Avian Dose-Based Risk Quotients

EECs and RQs

Short Grass Tall Grass Broadleaf Plants/ Small Insects Fruits/Pods/ Seeds/ Large Insects Granivore Size Class (grams) Adjusted LD50

EEC RQ EEC RQ EEC RQ EEC RQ EEC RQ

20 1038.45 77.69 0.07 35.61 0.03 43. 70 0.04 4.86 0.00 1.08 0.00

100 1322.00 44.30 0.03 20.31 0.02 24. 92 0.02 2.77 0.00 0.62 0.00

1000 1867.37 19.84 0.01 9.09 0.00 11. 16 0.01 1.24 0.00 0.28 0.00

Upper Bound Kenaga, Subacute Avian Dietary Based Risk Quotients EECs and RQs

Short Grass Tall Grass Broadleaf Plants/ Small Insects Fruits/Pods/ Seeds/ Large Insects

LC50 EEC RQ EEC RQ EEC RQ EEC RQ

5050 68.22 0.01 31.27 0.01 38.37 0.01 4.26 0.00

Size class not used for dietary risk quotients

Upper Bound Kenaga, Chronic Avian Dietary Based Risk Quotients

EECs and RQs

Short Grass Tall Grass Broadleaf Plants/ Small Insects Fruits/Pods/ Seeds/ Large Insects

NOAEC (ppm) EEC RQ EEC RQ EEC RQ EEC RQ

320 68.22 0.21 31.27 0.10 38.37 0.12 4.26 0.01 Size class not used for dietary risk quotients

### 19 Upper Bound Kenaga, Acute Mammalian Dose-Based Risk Quotients

EECs and RQs

Short Grass Tall Grass Broadleaf Plants/ Small Insects Fruits/Pods/ Seeds/ Large Insects Granivore Size Class (grams) Adjusted LD50

EEC RQ EEC RQ EEC RQ EEC RQ EEC RQ

15 10989.15 65.04 0.01 29.81 0.00 36. 58 0.00 4.06 0.00 0.90 0.00

35 8891.40 44.95 0.01 20.60 0.00 25. 28 0.00 2.81 0.00 0.62 0.00

1000 3845.80 10.42 0.00 4.78 0.00 5. 86 0.00 0.65 0.00 0.14 0.00

Upper Bound Kenaga, Chronic Mammalian Dietary Based Risk Quotients EECs and RQs

Short Grass Tall Grass Broadleaf Plants/ Small Insects Fruits/Pods/ Seeds/ Large Insects NOAEC (ppm)

EEC RQ EEC RQ EEC RQ EEC RQ

1500 68.22 0.05 31.27 0.02 38.37 0.03 4.26 0.00 Size class not used for dietary risk quotients

Upper Bound Kenaga, Chronic Mammalian Dose-Based Risk Quotients EECs and RQs

Short Grass Tall Grass Broadleaf Plants/ Small Insects Fruits/Pods/ Seeds/ Large Insects Granivore Size Class (grams) Adjusted NOAEL

EEC RQ EEC RQ EEC RQ EEC RQ EEC RQ

15 164.84 65.04 0.39 29.81 0.18 36. 58 0.22 4.06 0.02 0.90 0.01

35 133.37 44.95 0.34 20.60 0.15 25. 28 0.19 2.81 0.02 0.62 0.00

1000 57.69 10.42 0.18 4.78 0.08 5. 86 0.10 0.65 0.01 0.14 0.00