### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:17:46 AM] You are here: EPA Home Prevention, Pesticides & Toxic Substances Pollution Prevention & Toxics High Production Volume (HPV) Challenge High Production Volume Information System (HPVIS) Submission Report 

# High Production Volume Information System (HPVIS)

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# Submission Report

Submission Information

Submission ID : 25349374 Submission Name : Butanedioic acid, tetrapropenylAR Number :



## Chemical Information

CAS Number : 27859-58-1 Ninth Collective Index Name : Butanedioic acid, (tetrapropenyl)-



## Submitter Information

Submitter's Name : EPA

Address : City : Washington

State : DC
ZIP/Postal Code : Country : Type of Submitter : Company 

## Technical Point of Contact Information

Technical Point of Contact :
Title :
Phone Number :
Fax Number :
E-Mail Address : 

## Additional Technical Point of Contact Information

Technical Point of Contact :
Title :
Phone Number :
Fax Number :
E-Mail Address :



## Sponsor Information
Sponsor's Name : EPA

Address : City :
State : ZIP/Postal Code : Country :
Phone Number :
Fax Number : Share

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:17:46 AM] E-Mail Address : Type of Sponsor : Company 

## Technical Point of Contact Information

Technical Point of Contact :
Title :
Phone Number :
Fax Number :
E-Mail Address :



## Acute Toxicity to Aquatic Vertebrates

CAS Number : 27859-58-1

Sponsored Chemical Name : Butanedioic acid, (tetrapropenyl)-



### Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (27859-58-1) Butanedioic acid, (tetrapropenyl)-

Test Substance Purity/Composition and Other Test Substance Comments:
Sponsored Chemical Result Type:

Test Substance Result Type: 

### Method - Acute Toxicity To Aquatic Vertebrates

Year Study Performed:

Method/Guideline Followed:

Other Method/Guideline:

Deviations from Method/Guideline:
Species:

GLP:

Analytical Monitoring:
Test Type:

Test Vessel:

Water Media Type:

Test Concentrations:

Nominal and Measured Concentrations:
Exposure Period:

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:17:46 AM] Vehicle Used:

Vehicle Name:

Vehicle Amount and Units:
Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature and Units:

Photo (Light/Dark):
Salinity:

TOC:

Water Hardness:

Method/Guideline Test Conditions Remarks:

Limit Test:



### Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure Duration:

NOEC:

LOEC Exposure Duration:

LOEC:

NOELR Exposure Duration:

NOELR:

LOELR Exposure Duration:

LOELR:

LC/EC Mean Value : Exposure Duration Exposure Units LC/EC % Value Description Mean Value

or Lower Mean Value Upper Mean Value Units Effect Observed Basis for Concentration

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:17:46 AM] Results Remarks:



### Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability:

Reliability Remarks: As of February 2010, no data were available for this endpoint.

Key Study Sponsor Indicator: 

### Reference - Acute Toxicity To Aquatic Vertebrates

Reference:



## Acute Toxicity to Aquatic Invertebrates

CAS Number : 27859-58-1

Sponsored Chemical Name : Butanedioic acid, (tetrapropenyl)-



### Test Substance - Acute Toxicity To Aquatic Invertebrates

Test Substance: (27859-58-1) Butanedioic acid, (tetrapropenyl)-

Test Substance Purity/Composition and Other Test Substance Comments:
Sponsored Chemical Result Type:

Test Substance Result Type: 

### Method - Acute Toxicity To Aquatic Invertebrates

Year Study Performed:

Method/Guideline Followed:

Other Method/Guideline:

Deviations from Method/Guideline:
Species/In Vitro System:

GLP:

Analytical Monitoring:
Test Type:

Test Vessel:

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:17:46 AM] Water Media Type:

Test Concentrations:

Nominal and Measured Concentrations:
Exposure Period:

Vehicle Used:

Vehicle Name:

Vehicle Amount and Units:
Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature and Units:

Photo (Light/Dark):
Salinity:

TOC:

Water Hardness:

Method/Guideline Test Conditions Remarks:

Limit Test:



### Test Results - Acute Toxicity To Aquatic Invertebrates

NOEC Exposure Duration:

NOEC:

LOEC Exposure Duration:

LOEC:

NOELR Exposure Duration:

NOELR:

LOELR Exposure Duration:

LOELR:

LC/EC Mean Value : Exposure Duration Exposure Units LC/EC % Value Description Mean Value

or Upper Mean Value Units Effect Observed Basis for Concentration

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:17:46 AM] Lower Mean Value

Results Remarks:



### Reliability/Data Quality - Acute Toxicity To Aquatic Invertebrates

Reliability:

Reliability Remarks: As of February 2010, no data were available for this endpoint.

Key Study Sponsor Indicator: 

### Reference - Acute Toxicity To Aquatic Invertebrates

Reference:



## Acute Toxicity to Aquatic Plants

CAS Number : 27859-58-1

Sponsored Chemical Name : Butanedioic acid, (tetrapropenyl)-



### Test Substance - Acute Toxicity To Aquatic Plants

Test Substance: (27859-58-1) Butanedioic acid, (tetrapropenyl)-

Test Substance Purity/Composition and Other Test Substance Comments: Purity not provided

Sponsored Chemical Result Type: Measured

Test Substance Result Type: Nominal



### Method - Acute Toxicity To Aquatic Plants

Year Study Performed: 1996

Method/Guideline Followed: OECD 201

Other Method/Guideline: US EPA Toxic Substance Control Act Test Guideline No. 797.1050 (1993) and OECD TG No. 201 Alga, Growth Inhibition Test (1984)

Deviations from Method/Guideline: No Data

Species: Pseudokirchnerella subcapitata

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:17:46 AM] GLP: Yes

Analytical Monitoring: No

Test Type: Static

Test Vessel: Closed

Water Media Type: Freshwater

Test Concentrations: Other

Nominal and Measured Concentrations: WAF loading rates: 0 (control), 0.3, 3.0, 33, 330 and 3300 mg/L

Exposure Period: 96 Hours

Vehicle Used: No

Vehicle Name:

Vehicle Amount and Units:
Alkalinity:

Dissolved Oxygen:

pH Value: Value/Lower Range: = 7.4 Upper Range: 10.2

Test Temperature and Units: Value/Lower Range: 24 °C

Photo (Light/Dark):
Salinity:

TOC:

Water Hardness:

Method/Guideline Test Conditions Remarks: Element basis (#of cells/mL): Approximately 10,000 cells/mL

Statistical methods: Average specific growth rate was calculated as the natural log of the number of cells/ml at 72 and 96 hours minus the natural log of the number of cells/ml at 0 hour, divided by the hour of exposure. Results were interpreted by standard statistical techniques. All calculations were performed using nominal concentrations of the test material with the number of cells/mL, then with the average specific growth rates.

Test Species: Cells taken from in-house culture Pseudokirchneriella subcapitata originally purchased from the University of Texas at Austin alga collection.

Test System: Each WAF was prepared only at the beginning of the test. A measured weight of test material was added to a measured volume of dilution water in a glass vessel and stirred for 20 hours. Stirring accomplished using a magnetic stirrer. Mixing speed was adjusted such that a vortex formed approximately 25% of the distance to the bottom. Following the mixing period, the test solution was allowed to stand for 4 hour before the water phase was removed. The siphoned water phase (i.e. WAF) was used for the aquatic toxicity test.

A static test was conducted; i.e. there was no daily renewal of test solution. Two

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:17:46 AM] 100-mL replicates per treatment, inoculum ~10,000 cells/mL. The 250-mL Erlenmeyer flasks were covered to reduce entry of dust. During the test all treatment and control flasks were randomly placed on an orbital shaker adjusted to approximately 100 cycles per minute under constant light (24 hours/day). The occurrence of relative size differences, unusual cell shapes, colors, flocculations, adherence of cells to test containers or aggregation of cells was determined visually by means of direct microscopic examination with a hemocytometer. Cell counts were made at
72 and 96 hours.

Light: Cool-white fluorescent lights provided a light intensity of approximately
400-430 foot-candles.

Dilution Water: Sterile enriched alga growth media (US EPA, 1978) adjusted to pH 7.5. Measured total suspended solids in fresh untreated alga media were <10 mg/L, respectively. Test media pH was 7.4 at 0-hour and 10.2 after 96 hours.

Limit Test:



### Test Results - Acute Toxicity To Aquatic Plants

NOEC Exposure Duration: 96 Hours

NOEC: = 33 mg/L Nominal

LOEC Exposure Duration:

LOEC:

NOELR Exposure Duration:

NOELR:

LOELR Exposure Duration:

LOELR:

Effect : Exposure Duration Exposure Units Type % Value Description Mean Value

or Lower Mean Value Upper Mean Value Units Basis for Effect Basis for Concentration

72 Hours EC 50 % = 100 mg/L Cell Number Nominal

96 Hours EC 50 % = 93 mg/L Cell Number Nominal

72 Hours EC 50 % = 100 mg/L Growth Rate Nominal

96 Hours EC 50 % = 100 mg/L Growth Rate Nominal

Results Remarks: Insoluble material was observed at 24, 48 and 96 hours in test vessels containing 330 and 3300 mg/L. No other insoluble material was observed during the study. The algal population grew well resulting in an average of 1,508,000 cells/mL in the control after 96 hours. Water quality was acceptable throughout the study. The two highest concentrations of test material significantly decreased the pH of the test media at the beginning of the test (330 mg/L pH: 4.3-4.4; 3000 mg/L pH: 3.9-4.0. No biological effects were noted during the study on cell size, shape, color, flocculation, adherence to test containers or aggregation.

The 96-hour no observed effect concentration (NOEC) was 33 mg/L. The calculated
EC50s were as follows:

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:17:46 AM] Based on Number of Cells/mL 72 hr EC50: 100 mg/L (95%confidence interval 33-330 mg/L) 96 hr EC50: 93 mg/L (95%confidence interval 33-330 mg/L)

Based on Average Specific Growth Rate 72 hr EC50: 100 mg/L (95%confidence interval 33-330 mg/L) 96 hr EC50:100 mg/L (95%confidence interval 33-330 mg/L)

*The toxic effects were determined to be algistatic based on the rapid re-growth of an aliquot of cells taken from the 330mg/L test vessel and cultured in fresh control media

Test Validity: Control response was satisfactory.

Conclusion: The test material was considered algistatic to freshwater alga at loading rates of 330 and 3000 mg/L. The 96-h EC50 = 93 mg/L; 96-hr NOEC = 33 mg/L. 

### Reliability/Data Quality - Acute Toxicity To Aquatic Plants

Reliability: 2 - Valid With Restrictions

Reliability Remarks: Restriction due to the lack of any analytical confirmation of test material concentration in test solutions. All concentrations are expressed as nominal.
Key Study Sponsor Indicator: 

### Reference - Acute Toxicity To Aquatic Plants

Reference: Confidential business information, taken from ACC HERTG panel Robust Summary "Alkenyl Succinic Anhydrides", Update Nov. 22, 2002



## Acute Toxicity

CAS Number : 27859-58-1

Sponsored Chemical Name

: Butanedioic acid, (tetrapropenyl)-



### Test Substance - Acute Toxicity

Test Substance: (27859-58-1) Butanedioic acid, (tetrapropenyl)-

Test Substance Purity/Composition and Other Test Substance Comments:
Sponsored Chemical Result Type: 

### Method - Acute Toxicity

Route of Administration:

Type of Exposure:

Species:

Mammalian Strain:

Gender:

Number of Animals per

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:17:46 AM] Dose:

Dose:

Year Study Performed:

Method/Guideline Followed:

GLP:

Method/Guideline and Test Condition Remarks: 

### Test Results - Acute Toxicity
Concentration (LC/LD) : LC/LD % Value Description Value/Lower Concentration Upper Concentration Units

Number of Deaths ( Male ):
Number of Deaths ( Female ):
Number of Deaths ( Total ):

Results Remarks:

Conclusion:



### Reliability/Data Quality - Acute Toxicity

Reliability:

Reliability Remarks: As of February 2010, no data were available for this endpoint.
Key Study Sponsor Indicator: 

### Reference - Acute Toxicity

Reference:



## Repeated-Dose Toxicity

CAS Number : 27859-58-1

Sponsored Chemical Name : Butanedioic acid, (tetrapropenyl)-



### Test Substance - Repeated-Dose Toxicity

Test Substance: (27859-58-1) Butanedioic acid, (tetrapropenyl)-

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:17:46 AM] Test Substance Purity/Composition and Other Test Substance Comments:
Sponsored Chemical Result Type: 

### Method - Repeated-Dose Toxicity

Route of Administration:
Type of Exposure:

Species:

Mammalian Strain:

Gender:

Number of Animals per Dose:

Dose:

Year Study Performed:

Method/Guideline Followed:

GLP:

Exposure Period:

Frequency of Treatment:
Post-Exposure Period:

Method/Guideline and Test Condition Remarks: 

### Test Results - Repeated-Dose Toxicity
Concentration (LOAEL/ LOAEC/ NOAEL/ NOAEC) : LOAEL/ LOAEC/ NOAEL/ NOAEC Population Value Description Value/Lower Concentration Upper Concentration Units

Results Remarks:

Conclusion:



### Reliability/Data Quality - Repeated-Dose Toxicity

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:17:46 AM] Reliability:

Reliability Remarks: As of February 2010, no data were available for this endpoint.

Key Study Sponsor Indicator: 

### Reference - Repeated-Dose Toxicity

Reference:



## Genetic Toxicity in vivo

CAS Number : 27859-58-1

Sponsored Chemical Name

: Butanedioic acid, (tetrapropenyl)-



### Test Substance - Genetic Toxicity in vivo

Test Substance: (27859-58-1) Butanedioic acid, (tetrapropenyl)-

Test Substance Purity/Composition and Other Test Substance Comments:
Sponsored Chemical Result Type: 

### Method - Genetic Toxicity in vivo

Type of Study:

Type of Test:

Route of Administration:

Type of Exposure:

Species:

Strain:

Gender:

Dose:

Year Study Performed:

Method/Guideline Followed:

GLP:

Duration of Treatment/ Exposure Period and Units:
Frequency of Treatment:

Positive, Negative, and Solvent

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:17:46 AM] Control Substance(s):
Number of Animals per Sex and Dose Group:

Method/Guideline and Test Condition Remarks: 

### Test Results - Genetic Toxicity in vivo

Systemic Toxicity:

Genotoxic Effect:

Results Remarks:

Conclusion:



### Reliability/Data Quality - Genetic Toxicity in vivo

Reliability:

Reliability Remarks: As of February 2010, no data were available for this endpoint.
Key Study Sponsor Indicator: 

### Reference - Genetic Toxicity in vivo

Reference:



## Genetic Toxicity in vitro

CAS Number : 27859-58-1

Sponsored Chemical Name

: Butanedioic acid, (tetrapropenyl)-



### Test Substance - Genetic Toxicity in vitro

Test Substance: (27859-58-1) Butanedioic acid, (tetrapropenyl)-

Test Substance Purity/Composition and Other Test Substance Comments:
Sponsored Chemical Result Type: 

### Method - Genetic Toxicity in vitro

Type of Study:

Concentrations:

Year Study Performed:

Method/Guideline Followed:

GLP:

Postive, Negative, and Solvent Control Substance(s):

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:17:46 AM] Method/Guideline and Test Condition Remarks: 

### Test Results - Genetic Toxicity in vitro
Details on Cytogenetic Assay:

Statistics:

Effect : Species Other Species Strain Other Strain Metabolic Activation Genotoxic Effect Conclusion

Results Remarks:

Conclusion:



### Reliability/Data Quality - Genetic Toxicity in vitro

Reliability:

Reliability Remarks: As of February 2010, no data were available for this endpoint.
Key Study Sponsor Indicator: 

### Reference - Genetic Toxicity in vitro

Reference:



## Reproductive Toxicity

CAS Number : 27859-58-1

Sponsored Chemical Name : Butanedioic acid, (tetrapropenyl)-



### Test Substance - Reproductive Toxicity

Test Substance: (27859-58-1) Butanedioic acid, (tetrapropenyl)-

Test Substance Purity/Composition and Other Test Substance Comments:
Sponsored Chemical Result Type: 

### Method - Reproductive Toxicity

Route of

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:17:46 AM] Administration:
Type of Exposure:

Species:

Mammalian Strain:

Gender:

Number of Animals per Dose:

Dose:

Year Study Performed:

Method/Guideline Followed:

GLP:

Exposure Period:

Frequency of Treatment:
Post-Exposure Period:

Method/Guideline and Test Condition Remarks:
Pre-Mating Exposure / Males:
Pre-Mating Exposure / Females: 

### Test Results - Reproductive Toxicity
Concentration (LOAEL/ LOAEC/ NOAEL/ NOAEC) : LOAEL/ LOAEC/ NOAEL/ NOAEC Population Value Description Value/Lower Concentration Upper Concentration Units

Results:

Results Remarks:

Conclusion:



### Reliability/Data Quality - Reproductive Toxicity

Reliability:

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:17:46 AM] Reliability Remarks: As of February 2010, no data were available for this endpoint.

Key Study Sponsor Indicator: 

### Reference - Reproductive Toxicity

Reference:



## Developmental Toxicity/Teratogenicity

CAS Number : 27859-58-1

Sponsored Chemical Name : Butanedioic acid, (tetrapropenyl)-



### Test Substance - Developmental Toxicity/Teratogenicity

Test Substance: (27859-58-1) Butanedioic acid, (tetrapropenyl)-

Test Substance Purity/Composition and Other Test Substance Comments:
Sponsored Chemical Result Type: 

### Method - Developmental Toxicity/Teratogenicity

Route of Administration:
Type of Exposure:

Species:

Mammalian Strain:

Gender:

Number of Animals per Dose:

Dose:

Year Study Performed:

Method/Guideline Followed:

GLP:

Exposure Period:

Frequency of Treatment:
Post-Exposure Period:

Method/Guideline and Test Condition Remarks: 

### Test Results - Developmental Toxicity/Teratogenicity

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:17:46 AM] EPA Home Privacy and Security Notice Contact Us
http://iaspub.epa.gov/hpvis/ServSubmissionPrint Print As-Is
Last updated on Wednesday, June 15, 2011 Concentration (LOAEL/ LOAEC/ NOAEL/ NOAEC) : LOAEL/ LOAEC/ NOAEL/ NOAEC Population Value Description Value/Lower Concentration Upper Concentration Units

Results Remarks:

Conclusion:



### Reliability/Data Quality - Developmental Toxicity/Teratogenicity

Reliability:

Reliability Remarks: As of February 2010, no data were available for this endpoint.

Key Study Sponsor Indicator: 

### Reference - Developmental Toxicity/Teratogenicity

Reference: