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Submission Information

Submission ID : 25349374 Submission Name : Butanedioic acid, tetrapropenylAR Number : 

Chemical Information

CAS Number : 27859-58-1 Ninth Collective Index Name : Butanedioic acid, (tetrapropenyl)-

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 Acute Toxicity to Aquatic Vertebrates

CAS Number : 27859-58-1

Sponsored

Chemical Name :

Butanedioic acid, (tetrapropenyl)-

Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (27859-58-1) Butanedioic acid, (tetrapropenyl)-

Test Substance

Purity/Composition

and Other Test

Substance

Comments:

Sponsored

Chemical Result

Type:

Test Substance

Result Type:

Method - Acute Toxicity To Aquatic Vertebrates

Year Study

Performed:

Method/Guideline

Followed:

Other

Method/Guideline:

Deviations from

Method/Guideline:

Species:

GLP:

Analytical

Monitoring:

Test Type:

Test Vessel:

Water Media Type:

Test

Concentrations:

Nominal and

Measured

Concentrations:

Exposure Period:
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Vehicle Used:

Vehicle Name:

Vehicle Amount

and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature

and Units:

Photo

(Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline

Test Conditions

Remarks:

Limit Test:

Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure

Duration:

NOEC:

LOEC Exposure

Duration:

LOEC:

NOELR Exposure

Duration:

NOELR:

LOELR Exposure

Duration:

LOELR:

LC/EC Mean Value : Exposure

Duration

Exposure

Units

LC/EC % Value

Description

Mean

Value

or

Lower

Mean

Value

Upper

Mean

Value

Units Effect

Observed

Basis for

Concentration
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Results Remarks:

Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability:

Reliability

Remarks:

As of February 2010, no data were available for this endpoint.

Key Study Sponsor

Indicator:

Reference - Acute Toxicity To Aquatic Vertebrates

Reference:

 Acute Toxicity to Aquatic Invertebrates

CAS Number : 27859-58-1

Sponsored

Chemical Name :

Butanedioic acid, (tetrapropenyl)-

Test Substance - Acute Toxicity To Aquatic Invertebrates

Test Substance: (27859-58-1) Butanedioic acid, (tetrapropenyl)-

Test Substance

Purity/Composition

and Other Test

Substance

Comments:

Sponsored

Chemical Result

Type:

Test Substance

Result Type:

Method - Acute Toxicity To Aquatic Invertebrates

Year Study

Performed:

Method/Guideline

Followed:

Other

Method/Guideline:

Deviations from

Method/Guideline:

Species/In Vitro

System:

GLP:

Analytical

Monitoring:

Test Type:

Test Vessel:
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Water Media Type:

Test

Concentrations:

Nominal and

Measured

Concentrations:

Exposure Period:

Vehicle Used:

Vehicle Name:

Vehicle Amount

and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature

and Units:

Photo

(Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline

Test Conditions

Remarks:

Limit Test:

Test Results - Acute Toxicity To Aquatic Invertebrates

NOEC Exposure

Duration:

NOEC:

LOEC Exposure

Duration:

LOEC:

NOELR Exposure

Duration:

NOELR:

LOELR Exposure

Duration:

LOELR:

LC/EC Mean Value : Exposure

Duration

Exposure

Units

LC/EC % Value

Description

Mean

Value

or

Upper

Mean

Value

Units Effect

Observed

Basis for

Concentration
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

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Lower

Mean

Value

Results Remarks:

Reliability/Data Quality - Acute Toxicity To Aquatic Invertebrates

Reliability:

Reliability

Remarks:

As of February 2010, no data were available for this endpoint.

Key Study Sponsor

Indicator:

Reference - Acute Toxicity To Aquatic Invertebrates

Reference:

 Acute Toxicity to Aquatic Plants

CAS Number : 27859-58-1

Sponsored

Chemical Name :

Butanedioic acid, (tetrapropenyl)-

Test Substance - Acute Toxicity To Aquatic Plants

Test Substance: (27859-58-1) Butanedioic acid, (tetrapropenyl)-

Test Substance

Purity/Composition

and Other Test

Substance

Comments:

Purity not provided 

Sponsored

Chemical Result

Type:

Measured

Test Substance

Result Type:

Nominal

Method - Acute Toxicity To Aquatic Plants

Year Study

Performed:

1996

Method/Guideline

Followed:

OECD 201

Other

Method/Guideline:

US EPA Toxic Substance Control Act Test Guideline No. 797.1050 (1993) and OECD TG

No. 201 Alga, Growth Inhibition Test (1984)

Deviations from

Method/Guideline:

No Data

Species: Pseudokirchnerella subcapitata
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GLP: Yes

Analytical

Monitoring:

No

Test Type: Static

Test Vessel: Closed

Water Media Type: Freshwater

Test

Concentrations:

Other

Nominal and

Measured

Concentrations:

WAF loading rates: 0 (control), 0.3, 3.0, 33, 330 and 3300 mg/L 

Exposure Period: 96 Hours

Vehicle Used: No

Vehicle Name:

Vehicle Amount

and Units:

Alkalinity:

Dissolved Oxygen:

pH Value: Value/Lower Range: = 7.4 Upper Range: 10.2

Test Temperature

and Units:

Value/Lower Range: 24 °C

Photo

(Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline

Test Conditions

Remarks:

Element basis (#of cells/mL): Approximately 10,000 cells/mL

Statistical methods: Average specific growth rate was calculated as the natural

log of the number of cells/ml at 72 and 96 hours minus the natural log of the

number of cells/ml at 0 hour, divided by the hour of exposure. Results were

interpreted by standard statistical techniques. All calculations were performed

using nominal concentrations of the test material with the number of cells/mL,

then with the average specific growth rates.

Test Species: Cells taken from in-house culture Pseudokirchneriella subcapitata

originally purchased from the University of Texas at Austin alga collection.

Test System: Each WAF was prepared only at the beginning of the test. A measured

weight of test material was added to a measured volume of dilution water in a

glass vessel and stirred for 20 hours. Stirring accomplished using a magnetic

stirrer. Mixing speed was adjusted such that a vortex formed approximately 25% of

the distance to the bottom. Following the mixing period, the test solution was

allowed to stand for 4 hour before the water phase was removed. The siphoned water

phase (i.e. WAF) was used for the aquatic toxicity test.

A static test was conducted; i.e. there was no daily renewal of test solution. Two
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100-mL replicates per treatment, inoculum ~10,000 cells/mL. The 250-mL Erlenmeyer

flasks were covered to reduce entry of dust. During the test all treatment and

control flasks were randomly placed on an orbital shaker adjusted to approximately

100 cycles per minute under constant light (24 hours/day). The occurrence of

relative size differences, unusual cell shapes, colors, flocculations, adherence of

cells to test containers or aggregation of cells was determined visually by means

of direct microscopic examination with a hemocytometer. Cell counts were made at

72 and 96 hours.

Light: Cool-white fluorescent lights provided a light intensity of approximately

400-430 foot-candles.

Dilution Water: Sterile enriched alga growth media (US EPA, 1978) adjusted to pH

7.5. Measured total suspended solids in fresh untreated alga media were <10 mg/L,

respectively. Test media pH was 7.4 at 0-hour and 10.2 after 96 hours.

Limit Test:

Test Results - Acute Toxicity To Aquatic Plants

NOEC Exposure

Duration:

96 Hours

NOEC: = 33 mg/L Nominal

LOEC Exposure

Duration:

LOEC:

NOELR Exposure

Duration:

NOELR:

LOELR Exposure

Duration:

LOELR:

Effect : Exposure

Duration

Exposure

Units

Type % Value

Description

Mean

Value

or

Lower

Mean

Value

Upper

Mean

Value

Units Basis

for

Effect

Basis for

Concentration

72 Hours EC 50 % = 100 mg/L Cell

Number

Nominal

96 Hours EC 50 % = 93 mg/L Cell

Number

Nominal

72 Hours EC 50 % = 100 mg/L Growth

Rate

Nominal

96 Hours EC 50 % = 100 mg/L Growth

Rate

Nominal

Results Remarks: Insoluble material was observed at 24, 48 and 96 hours in test vessels containing

330 and 3300 mg/L. No other insoluble material was observed during the study. The

algal population grew well resulting in an average of 1,508,000 cells/mL in the

control after 96 hours. Water quality was acceptable throughout the study. The two

highest concentrations of test material significantly decreased the pH of the test

media at the beginning of the test (330 mg/L pH: 4.3-4.4; 3000 mg/L pH: 3.9-4.0.

No biological effects were noted during the study on cell size, shape, color,

flocculation, adherence to test containers or aggregation.

The 96-hour no observed effect concentration (NOEC) was 33 mg/L. The calculated

EC50s were as follows:
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Based on Number of Cells/mL

72 hr EC50: 100 mg/L (95%confidence interval 33-330 mg/L)

96 hr EC50: 93 mg/L (95%confidence interval 33-330 mg/L)

Based on Average Specific Growth Rate

72 hr EC50: 100 mg/L (95%confidence interval 33-330 mg/L)

96 hr EC50:100 mg/L (95%confidence interval 33-330 mg/L)

*The toxic effects were determined to be algistatic based on the rapid re-growth

of an aliquot of cells taken from the 330mg/L test vessel and cultured in fresh

control media

Test Validity: Control response was satisfactory.

Conclusion: The test material was considered algistatic to freshwater alga at

loading rates of 330 and 3000 mg/L. The 96-h EC50 = 93 mg/L; 96-hr NOEC = 33

mg/L.

Reliability/Data Quality - Acute Toxicity To Aquatic Plants

Reliability: 2 - Valid With Restrictions

Reliability

Remarks:

Restriction due to the lack of any analytical confirmation of test material 

concentration in test solutions. All concentrations are expressed as nominal. 

Key Study Sponsor

Indicator:

Reference - Acute Toxicity To Aquatic Plants

Reference: Confidential business information, taken from ACC HERTG panel Robust Summary

"Alkenyl Succinic Anhydrides", Update Nov. 22, 2002 

 Acute Toxicity

CAS Number : 27859-58-1

Sponsored Chemical Name

:

Butanedioic acid, (tetrapropenyl)-

Test Substance - Acute Toxicity

Test Substance: (27859-58-1) Butanedioic acid, (tetrapropenyl)-

Test Substance

Purity/Composition

and Other Test Substance

Comments:

Sponsored Chemical Result

Type:

Method - Acute Toxicity

Route of Administration:

Type of Exposure:

Species:

Mammalian Strain:

Gender:

Number of Animals per
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

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Dose:

Dose:

Year Study Performed:

Method/Guideline

Followed:

GLP:

Method/Guideline and

Test Condition Remarks:

Test Results - Acute Toxicity

Concentration (LC/LD) : LC/LD % Value

Description

Value/Lower

Concentration

Upper

Concentration

Units

Number of Deaths ( Male

):

Number of Deaths ( Female

):

Number of Deaths ( Total

):

Results Remarks:

Conclusion:

Reliability/Data Quality - Acute Toxicity

Reliability:

Reliability Remarks: As of February 2010, no data were available for this

endpoint.

Key Study Sponsor

Indicator:

Reference - Acute Toxicity

Reference:

 Repeated-Dose Toxicity

CAS Number : 27859-58-1

Sponsored Chemical

Name :

Butanedioic acid, (tetrapropenyl)-

Test Substance - Repeated-Dose Toxicity

Test Substance: (27859-58-1) Butanedioic acid, (tetrapropenyl)-
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

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Test Substance

Purity/Composition

and Other Test

Substance Comments:

Sponsored Chemical

Result Type:

Method - Repeated-Dose Toxicity

Route of

Administration:

Type of Exposure:

Species:

Mammalian Strain:

Gender:

Number of Animals per

Dose:

Dose:

Year Study Performed:

Method/Guideline

Followed:

GLP:

Exposure Period:

Frequency of

Treatment:

Post-Exposure Period:

Method/Guideline and

Test Condition

Remarks:

Test Results - Repeated-Dose Toxicity

Concentration (LOAEL/

LOAEC/ NOAEL/

NOAEC) :

LOAEL/

LOAEC/

NOAEL/

NOAEC

Population Value

Description

Value/Lower

Concentration

Upper

Concentration

Units

Results Remarks:

Conclusion:

Reliability/Data Quality - Repeated-Dose Toxicity
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Reliability:

Reliability Remarks: As of February 2010, no data were available for this endpoint.

Key Study Sponsor

Indicator:

Reference - Repeated-Dose Toxicity

Reference:

 Genetic Toxicity in vivo

CAS Number : 27859-58-1

Sponsored Chemical Name

:

Butanedioic acid, (tetrapropenyl)-

Test Substance - Genetic Toxicity in vivo

Test Substance: (27859-58-1) Butanedioic acid, (tetrapropenyl)-

Test Substance

Purity/Composition

and Other Test Substance

Comments:

Sponsored Chemical Result

Type:

Method - Genetic Toxicity in vivo

Type of Study:

Type of Test:

Route of Administration:

Type of Exposure:

Species:

Strain:

Gender:

Dose:

Year Study Performed:

Method/Guideline

Followed:

GLP:

Duration of Treatment/

Exposure Period and

Units:

Frequency of Treatment:

Positive, Negative, and

Solvent
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Control Substance(s):

Number of Animals per Sex

and Dose Group:

Method/Guideline and

Test Condition Remarks:

Test Results - Genetic Toxicity in vivo

Systemic Toxicity:

Genotoxic Effect:

Results Remarks:

Conclusion:

Reliability/Data Quality - Genetic Toxicity in vivo

Reliability:

Reliability Remarks: As of February 2010, no data were available for this

endpoint.

Key Study Sponsor

Indicator:

Reference - Genetic Toxicity in vivo

Reference:

 Genetic Toxicity in vitro

CAS Number : 27859-58-1

Sponsored Chemical Name

:

Butanedioic acid, (tetrapropenyl)-

Test Substance - Genetic Toxicity in vitro

Test Substance: (27859-58-1) Butanedioic acid, (tetrapropenyl)-

Test Substance

Purity/Composition

and Other Test Substance

Comments:

Sponsored Chemical Result

Type:

Method - Genetic Toxicity in vitro

Type of Study:

Concentrations:

Year Study Performed:

Method/Guideline

Followed:

GLP:

Postive, Negative, and

Solvent

Control Substance(s):
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

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Method/Guideline and

Test Condition Remarks:

Test Results - Genetic Toxicity in vitro

Details on Cytogenetic

Assay:

Statistics:

Effect : Species Other

Species

Strain Other

Strain

Metabolic

Activation

Genotoxic

Effect

Conclusion

Results Remarks:

Conclusion:

Reliability/Data Quality - Genetic Toxicity in vitro

Reliability:

Reliability Remarks: As of February 2010, no data were available for this

endpoint.

Key Study Sponsor

Indicator:

Reference - Genetic Toxicity in vitro

Reference:

 Reproductive Toxicity

CAS Number : 27859-58-1

Sponsored Chemical

Name :

Butanedioic acid, (tetrapropenyl)-

Test Substance - Reproductive Toxicity

Test Substance: (27859-58-1) Butanedioic acid, (tetrapropenyl)-

Test Substance

Purity/Composition

and Other Test

Substance Comments:

Sponsored Chemical

Result Type:

Method - Reproductive Toxicity

Route of
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint[6/15/2011 11:17:46 AM]

Administration:

Type of Exposure:

Species:

Mammalian Strain:

Gender:

Number of Animals per

Dose:

Dose:

Year Study Performed:

Method/Guideline

Followed:

GLP:

Exposure Period:

Frequency of

Treatment:

Post-Exposure Period:

Method/Guideline and

Test Condition

Remarks:

Pre-Mating Exposure /

Males:

Pre-Mating Exposure /

Females:

Test Results - Reproductive Toxicity

Concentration (LOAEL/

LOAEC/ NOAEL/

NOAEC) :

LOAEL/

LOAEC/

NOAEL/

NOAEC

Population Value

Description

Value/Lower

Concentration

Upper

Concentration

Units

Results:

Results Remarks:

Conclusion:

Reliability/Data Quality - Reproductive Toxicity

Reliability:
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint[6/15/2011 11:17:46 AM]

Reliability Remarks: As of February 2010, no data were available for this endpoint.

Key Study Sponsor

Indicator:

Reference - Reproductive Toxicity

Reference:

 Developmental Toxicity/Teratogenicity

CAS Number : 27859-58-1

Sponsored Chemical

Name :

Butanedioic acid, (tetrapropenyl)-

Test Substance - Developmental Toxicity/Teratogenicity

Test Substance: (27859-58-1) Butanedioic acid, (tetrapropenyl)-

Test Substance

Purity/Composition

and Other Test

Substance Comments:

Sponsored Chemical

Result Type:

Method - Developmental Toxicity/Teratogenicity

Route of

Administration:

Type of Exposure:

Species:

Mammalian Strain:

Gender:

Number of Animals per

Dose:

Dose:

Year Study Performed:

Method/Guideline

Followed:

GLP:

Exposure Period:

Frequency of

Treatment:

Post-Exposure Period:

Method/Guideline and

Test Condition

Remarks:

Test Results - Developmental Toxicity/Teratogenicity
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Concentration (LOAEL/

LOAEC/ NOAEL/

NOAEC) :

LOAEL/

LOAEC/

NOAEL/

NOAEC

Population Value

Description

Value/Lower

Concentration

Upper

Concentration

Units

Results Remarks:

Conclusion:

Reliability/Data Quality - Developmental Toxicity/Teratogenicity

Reliability:

Reliability Remarks: As of February 2010, no data were available for this endpoint.

Key Study Sponsor

Indicator:

Reference - Developmental Toxicity/Teratogenicity

Reference: