using System;
using System.Collections.Generic;
using System.IO;
using System.Linq;
using static Libraries.Input;

namespace Libraries
{
    static class Input
    {
        private static Func<string, T[]> Cast<T>() => _ => _.Split(' ').Select(Convert<T>()).ToArray();

        private static Func<string, T> Convert<T>()
        {
            Type t = typeof(T);
            if (t == typeof(string)) return _ => (T) (object) _;
            if (t == typeof(int)) return _ => (T) (object) int.Parse(_);
            if (t == typeof(long)) return _ => (T) (object) long.Parse(_);
            if (t == typeof(double)) return _ => (T) (object) double.Parse(_);
            if (t == typeof(string[])) return _ => (T) (object) _.Split(' ');
            if (t == typeof(int[])) return _ => (T) (object) Cast<int>()(_);
            if (t == typeof(long[])) return _ => (T) (object) Cast<long>()(_);
            if (t == typeof(double[])) return _ => (T) (object) Cast<double>()(_);

            throw new NotSupportedException(t + "is not supported.");
        }

        private static T Convert<T>(string s) => Convert<T>()(s);

        private static string String() => Console.ReadLine();

        private static string[] String(long rowNumber) => new bool[rowNumber].Select(_ => String()).ToArray();

        public static void Cin<T>(out T a) => a = Convert<T>(String());

        public static void Cin<T1, T2>(out T1 a1, out T2 a2)
        {
            var v = String().Split(' ');
            set(v[0], out a1);
            set(v[1], out a2);
        }

        public static void Cin<T1, T2, T3>(out T1 a1, out T2 a2, out T3 a3)
        {
            var v = String().Split(' ');
            set(v[0], out a1);
            set(v[1], out a2);
            set(v[2], out a3);
        }

        public static void Cin<T1, T2, T3, T4>(out T1 a1, out T2 a2, out T3 a3, out T4 a4)
        {
            var v = String().Split(' ');
            set(v[0], out a1);
            set(v[1], out a2);
            set(v[2], out a3);
            set(v[3], out a4);
        }

        public static void Cin<T1, T2, T3, T4, T5>(out T1 a1, out T2 a2, out T3 a3, out T4 a4, out T5 a5)
        {
            var v = String().Split(' ');
            set(v[0], out a1);
            set(v[1], out a2);
            set(v[2], out a3);
            set(v[3], out a4);
            set(v[4], out a5);
        }

        public static void Cin<T1, T2, T3, T4, T5, T6>(out T1 a1, out T2 a2, out T3 a3, out T4 a4, out T5 a5, out T6 a6)
        {
            var v = String().Split(' ');
            set(v[0], out a1);
            set(v[1], out a2);
            set(v[2], out a3);
            set(v[3], out a4);
            set(v[4], out a5);
            set(v[5], out a6);
        }

        private static void set<T1>(string s, out T1 o1) => o1 = Convert<T1>(s);

        public static void Cin<T>(long n, out T[] l) => l = String(n).Select(Convert<T>()).ToArray();

        public static void Cin<T1, T2>(long n, out T1[] l1, out T2[] l2)
        {
            l1 = new T1[n];
            l2 = new T2[n];
            for (int i = 0; i < n; i++) Cin(out l1[i], out l2[i]);
        }

        public static void Cin<T1, T2, T3>(long n, out T1[] l1, out T2[] l2, out T3[] l3)
        {
            l1 = new T1[n];
            l2 = new T2[n];
            l3 = new T3[n];
            for (int i = 0; i < n; i++) Cin(out l1[i], out l2[i], out l3[i]);
        }

        public static void Cin<T1, T2, T3, T4>(long n, out T1[] l1, out T2[] l2, out T3[] l3, out T4[] l4)
        {
            l1 = new T1[n];
            l2 = new T2[n];
            l3 = new T3[n];
            l4 = new T4[n];
            for (int i = 0; i < n; i++) Cin(out l1[i], out l2[i], out l3[i], out l4[i]);
        }

        public static void Cin<T>(out T[] a) => a = Convert<T[]>(String());

        public static void Cin<T>(long h, out T[][] map) => map = String(h).Select(Convert<T[]>()).ToArray();
    }

    class Program
    {
        public static void Main(string[] args)
        {
            StreamWriter streamWriter = new StreamWriter(Console.OpenStandardOutput()) {AutoFlush = false};
            Console.SetOut(streamWriter);
            new Solver().Solve();
            Console.Out.Flush();
        }

        public static void Debug()
        {
            new Solver().Solve();
        }
    }

    class Solver
    {
        public void Solve()
        {
            int N;
            string S;
            Cin(out N);
            Cin(out S);
            int ans = 0;
            for (int i = 1; i < N - 1; i++)
            {
                HashSet<char> s = new HashSet<char>();
                for (int j = 0; j < i; j++)
                {
                    s.Add(S[j]);
                }

                HashSet<char> a = new HashSet<char>();
                int c = 0;
                for (int j = i; j < N; j++)
                {
                    if (!a.Contains(S[j]) && s.Contains(S[j]))
                    {
                        c++;
                        a.Add(S[j]);
                    }
                }

                ans = Math.Max(ans, c);
            }

            Console.WriteLine(ans);
        }
    }
}

Measured memory_bytes: 13396