#[allow(unused_imports)]
use std::char::from_digit;
#[allow(unused_imports)]
use std::cmp::{max, min};
#[allow(unused_imports)]
use std::collections::{BTreeMap, BTreeSet, BinaryHeap, HashMap, HashSet, VecDeque};
#[allow(unused_imports)]
use std::fs::File;
#[allow(unused_imports)]
use std::io::prelude::*;
#[allow(unused_imports)]
use std::io::{stdin, stdout, BufReader, BufWriter, Stdin, Stdout, Write};
#[allow(unused_imports)]
use std::mem::swap;
#[allow(unused_imports)]
use std::str::FromStr;

type BoxResult<T> = Result<T, Box<std::error::Error>>;

fn main() {
    let mut scanner = Scanner::new(stdin());
    let _n = scanner.read::<usize>().unwrap();
    let s = scanner.read_chars().unwrap();
    let mut former = vec![0; 26];
    let mut latter = vec![0; 26];
    for &c in s.iter() {
        let index = c as usize - 'a' as usize;
        latter[index] += 1;
    }
    let mut ans = 0;
    let mut crt = 0;
    for &c in s.iter() {
        let index = c as usize - 'a' as usize;
        former[index] += 1;
        latter[index] -= 1;
        if former[index] == 1 && latter[index] > 0 {
            crt += 1;
        }
        if latter[index] == 0 && former[index] > 1 {
            crt -= 1;
        }
        ans = max(crt, ans);
    }
    println!("{}", ans);
}

#[allow(dead_code)]
struct Scanner<R> {
    reader: BufReader<R>,
    buffer: String,
}
impl<R> Scanner<R>
where
    R: Read,
{
    #[allow(dead_code)]
    fn new(inner: R) -> Scanner<R> {
        let reader = BufReader::new(inner);
        let buffer = String::new();
        Scanner {
            reader: reader,
            buffer: buffer,
        }
    }
    #[allow(dead_code)]
    fn with_capacity(capacity: usize, inner: R) -> Scanner<R> {
        let reader = BufReader::with_capacity(capacity, inner);
        let buffer = String::new();
        Scanner {
            reader: reader,
            buffer: buffer,
        }
    }

    #[allow(dead_code)]
    fn read_line(&mut self) -> BoxResult<String> {
        self.reader.read_line(&mut self.buffer)?;
        let cloned = self.buffer.trim().to_string();
        self.buffer.clear();
        Ok(cloned)
    }

    #[allow(dead_code)]
    fn read<T>(&mut self) -> BoxResult<T>
    where
        T: FromStr,
        T::Err: std::fmt::Debug,
        T::Err: std::error::Error,
        T::Err: 'static,
    {
        self.reader.read_line(&mut self.buffer)?;
        let parsed = self.buffer.trim().parse()?;
        self.buffer.clear();
        Ok(parsed)
    }
    #[allow(dead_code)]
    fn read_vector<T>(&mut self) -> BoxResult<Vec<T>>
    where
        T: FromStr,
        T::Err: std::fmt::Debug,
        T::Err: std::error::Error,
        T::Err: 'static,
    {
        self.reader.read_line(&mut self.buffer)?;
        let parsed_vec = self
            .buffer
            .trim()
            .split_whitespace()
            .map(|s| s.parse())
            .collect::<Result<Vec<_>, _>>()?;
        self.buffer.clear();
        Ok(parsed_vec)
    }
    #[allow(dead_code)]
    fn read_matrix<T>(&mut self, lines: usize) -> BoxResult<Vec<Vec<T>>>
    where
        T: FromStr,
        T::Err: std::fmt::Debug,
        T::Err: std::error::Error,
        T::Err: 'static,
    {
        let mut parsed_matrix = vec![];
        for _ in 0..lines {
            self.reader.read_line(&mut self.buffer)?;
            parsed_matrix.push(
                self.buffer
                    .trim()
                    .split_whitespace()
                    .map(|s| s.parse())
                    .collect::<Result<Vec<_>, _>>()?,
            );
            self.buffer.clear();
        }
        Ok(parsed_matrix)
    }
    #[allow(dead_code)]
    fn read_chars(&mut self) -> BoxResult<Vec<char>> {
        self.reader.read_line(&mut self.buffer)?;
        let chars = self.buffer.trim().chars().collect::<Vec<_>>();
        self.buffer.clear();
        Ok(chars)
    }
}


Measured memory_bytes: 4352