microextraction (SPME), or stir bar sorptive extraction (SBSE). This change of format is not only a reduction in size but also implies changing the polymerization modes which, despite the small size of the devices, must make it possible to maintain sufficient binding capacity. The modification of the polymerization conditions is also required when developing MIPs for proteins because of their lack of stability in conventional polymerization solvents. Based on the works published over the past 2 years for numerous target molecules, as reported in Figure 1, this paper aims to review the new strategies of development of MIPs in different formats dedicated to various extraction methods (whose distribution in terms of published work is given in Figure 2), often treated independently in the previously mentioned reviews, for both small organic molecules and proteins for which there are high expectations for replacing antibodies.

Piehowski, P.D., Zhu, Y., Bramer, L.M., Stratton, K.G., Zhao, R., Orton, D.J., Moore, R.J., Yuan, J., Mitchell, H.D., Gao, Y., Webb-Robertson, B.-J.M., Dey, S.K., Kelly, R.T., Burnum-Johnson, K.E., 2020. Automated mass spectrometry imaging of over 2000 proteins from tissue sections at 100- μm spatial resolution. Nature Communications 11, 8.

https://doi.org/10.1038/s41467-019-13858-z Biological tissues exhibit complex spatial heterogeneity that directs the functions of multicellular organisms. Quantifying protein expression is essential for elucidating processes within complex biological assemblies. Imaging mass spectrometry (IMS) is a powerful emerging tool for mapping the spatial distribution of metabolites and lipids across tissue surfaces, but technical challenges have limited the application of IMS to the analysis of proteomes. Methods for probing the spatial distribution of the proteome have generally relied on the use of labels and/or antibodies, which limits multiplexing and requires a priori knowledge of protein targets. Past efforts to make spatially resolved proteome measurements across tissues have had limited spatial resolution and proteome coverage and have relied on manual workflows. Here, we demonstrate an automated approach to imaging that utilizes label-free nanoproteomics to analyze tissue voxels, generating quantitative cell-type- specific images for >2000 proteins with 100-µm spatial resolution across mouse uterine tissue sections preparing for blastocyst implantation.

Pietzsch, R., Tedeschi, L.R., Oliveira, D.M., dos Anjos, C.W.D., Vazquez, J.C., Figueiredo, M.F., 2020. Environmental conditions of deposition of the Lower Cretaceous lacustrine carbonates of the Barra Velha p Formation, Santos Basin (Brazil), based on stable carbon and oxygen isotopes: A continental record of CO 2 during the onset of the Oceanic Anoxic Event 1a (OAE 1a) interval? Chemical Geology 535, 119457.

http://www.sciencedirect.com/science/article/pii/S0009254119305868 This study presents new stable carbon and oxygen isotope data from Lower Cretaceous lacustrine carbonate rock samples recovered from a well drilled in the Santos Basin, offshore southeast Brazil. These samples represent a record of a continental environment just prior to the opening of the South Atlantic Ocean and the ultimate break-up of Gondwanaland. The geochemical data, along with carbonate mineralogy, indicate repeated cycles of lake level variation that could be attributed to climatic oscillations. Despite the absence of correlations 13 18 between δ C and δ O values, facies analysis and the isotopic and mineralogical data suggest that lake hydrology was essentially closed for most of the depositional interval studied here. The existence of persisting 13 18 trends of nearly constant δ C values with a spread in δ O values though, suggests long water residence times in the palaeolake, equilibrium between atmosphere and lake water CO , as well as significant evaporation of 2 water. The overall geological model that emerges unveils a more comprehensive picture of the depositional conditions that favoured the continuity of a significant carbonate factory in the middle of the Gondwanan continent, corroborating previous studies that suggested the lasting existence of a large and somewhat shallow endorheic lake in the area during the Early Cretaceous. As a result of this recorded trend strongly suggesting equilibrium between lake waters DIC (dissolved inorganic carbon) reservoir and atmospheric CO , the data are 2 most consistent with lacustrine deposition rather than precipitation of travertine, contrasting with some suggestions for the genesis of the carbonates of the Barra Velha Formation. Finally, this apparent equilibrium with the atmosphere likely left a preserved record in the continental carbonates of the final stages that preceded a major global environmental disturbance associated with an increase in atmospheric CO , known for this time 2 as the Oceanic Anoxic Event (OAE) 1a. If this is correct, it also helps to put further time constraints on this