This form describes the methods for diluting pharmaceutical grade compounds or preparing non-pharmaceutical compounds for use in animals. The IACUC must review and approve the use of all compounds before they are used. For more information, see IACUC Policy 210 and the instructions on the preparation of compounds for use in animals in IACUC Guidance 608.

## Standard Operating Procedures Preparation of Compounds for Use in Animals

Text in red is either an example or an instruction. Please add, delete or modify, as appropriate.

Principal investigator	Name of compound	Species administered	Protocol #
Complete	Tribromoethanol anesthetic	Complete	Complete

### Active Ingredient(s)

Name	Chemical Grade* (FDA, USP, Analytical or Other)
2,2,2-tribromoethanol	Analytical

* FDA refers to drugs labelled for either human or veterinary use.

### Diluent(s)

[update diluent as needed]

Name	Chemical Grade** (FDA, USP, Analytical or Other)
2-methyl-2-butanol	Analytical
Phosphate buffered saline/saline	USP

** Note that diluents and inactive ingredients must also be FDA or USP grade, where possible. FDA/USP diluents must be used when compounding clinical drugs, such as anesthetic cocktails.

### Conditions for use:

Tribromoethanol (TBE) is an injectable anesthetic previously manufactured under the trade name Avertin®. However, this product is no longer available in pharmaceutical grade. In addition, TBE can cause a number of deleterious effects when administered to animals.

Due to the lack of a pharmaceutical grade drug and the potential for serious side effects, the IACUC does not recommend the use of TBE in animals. Therefore, the IACUC strongly encourages investigators to use alternate anesthetic agents, whenever possible. If the investigator is of the opinion that TBE is the anesthetic of choice, a scientific justification for its use must be provided.

If the use of TBE can be justified on scientific grounds and is approved by the IACUC, the following conditions must be met as part of that approval:

- • The TBE must be prepared, stored and disposed of in the manner described below
- • The dose must not exceed those given below
- • If approved for survival procedures, only a single dose must be administered
- • Any adverse or unexpected events must be immediately reported at: https://rgw.arizona.edu/compliance/IACUC/welfare-concerns/adverse-event

### Preparation:

Stock Solution:

- • Ensure that glassware and stirrers are clean by pre-treatment with 10% HCl to remove detergent residue and thoroughly rinsing with reagent grade H O. 2
- • Prepare the stock solution in a chemical fume hood, wearing nitrile gloves, a lab coat and safety glasses.
- • Add 6.2ml tertiary amyl alcohol (2 methyl-2-butanol) to 10g 2,2,2-tribromoethanol (TBE) in a capped container and stir until the TBE is dissolved. This may take several hours to overnight. Ensure the solution is kept away from light.
- • Label the bottle (stock solution), the date of preparation and a use-by date. The stock solution is stable for 6 months (use-by date).
- • • Store the solution at away from light and tightly capped, as the solution is photosensitive and hydroscopic.

I-IS-FM-003 Version: 003 Effective Date: 09/14/2018 Responsible Office/Title: IACUC Officer Pg. 1 of 2