User: 

You are given access to make searches on the Internet. The format for making a search is: 1. The user sends a message. 2. You will decide whether to make a search. If you choose to make a search, you will output a single message, containing the word 
 (EXACTLY AS IT IS GIVEN TO YOU), followed by ONLY your web search query. After outputting your search query, you MUST output the token 
 (EXACTLY AS IT IS GIVEN TO YOU) to denote the end of the search query. Keep your search query concise as there is a 400 characters limit.
The system will then append the web search results at the end of the user message, starting with the token 
. Take note that the user CANNOT ACCESS the content under 
 - YOU should incorporate these results into your response. Additionally, If the token 
 is present in the user's message, then you MUST NOT request another web search.

IMPORTANT! You should choose to search the web ONLY if ANY of the following circumstances are met:
1. User is asking about current events or something that requires real-time information (news, sports scores, 'latest' information, etc.)
2. User is asking about some term you are unfamiliar with (e.g. if it's a little-known term, if it's a person you don't know well, etc.)
3. User is asking about anything that involves numerical facts (e.g. distances between places, population count, sizes, date and time, etc.)
4. User explicitly asks you to search or provide links to references.
If the user's query does NOT require a web search, YOU MUST NEVER run one for no reason.
However, NEVER refuse to search the web if the user asks you to provide such information.

When using web search results, you are encouraged to cite references where appropriate, using inline links in standard markdown format.
You are also allowed to make use of web search results from previous messages, if they are STRONGLY RELEVANT to the current message.

When you are not running a web search, respond completely as per normal - there is NO NEED to mention that you're not searching. 
Do your best to be informative. However, you MUST NOT make up any information. If you think you might not know something, search for it.

Assistant: Understood. I will strictly follow these instructions in this conversation.
User: hi
User: What is the difference between DENO 1 and DENO 2
Assistant: Deno 1 and Deno 2 primarily differ in terms of features, performance improvements, and bug fixes. Deno 2 typically comes with enhancements over Deno 1, such as improved compatibility with web APIs, more efficient memory usage, better TypeScript support, and enhanced developer tooling. Additionally, Deno 2 might include new APIs and modules or deprecations of older features. For exact changes, it's best to refer to the official release notes of Deno.
User: Make it a list
Assistant: Certainly! Here's a list of differences between Deno 1 and Deno 2:

1. **Performance Improvements**: Deno 2 often includes optimizations for better performance and efficient memory usage compared to Deno 1.

2. **Web API Compatibility**: Deno 2 enhances compatibility with web standards, making it easier to work with web-based projects.

3. **TypeScript Support**: Improved TypeScript support with potentially faster compilation times and better handling of TypeScript features.

4. **Developer Tooling**: Enhanced developer tools for debugging and profiling applications, improving the development experience.

5. **New and Updated APIs**: Introduction of new APIs and modules, as well as updates to existing ones to provide more functionality.

6. **Deprecations**: Removal or deprecation of older features that are no longer necessary or have better alternatives in Deno 2.

7. **Bug Fixes**: Resolution of various bugs and issues identified in Deno 1, contributing to a more stable platform in Deno 2.

For detailed changes, the official Deno release notes serve as the best source of information.
User: What is the difference between DENO 1 and DENO 2
Assistant: Deno 1 and Deno 2 feature several differences, which can be summarized in a list:

1. **Performance Enhancements**: Deno 2 introduces optimizations for more efficient performance and memory management compared to Deno 1.

2. **Web Standard Compatibility**: There is improved alignment with web standards in Deno 2, aiding in seamless web-based project development.

3. **Advanced TypeScript Support**: Deno 2 offers better TypeScript integration, potentially resulting in faster compilation and improved feature handling.

4. **Enhanced Developer Tools**: The tools for debugging and profiling have been upgraded, offering a better developer experience in Deno 2.

5. **API Innovations**: New APIs and modules have been introduced, alongside updates to existing ones, providing more robust functionality in Deno 2.

6. **Feature Deprecations**: Deno 2 may deprecate or remove older features, replacing them with more effective alternatives to streamline operations.

7. **Bug Resolutions**: Various bugs present in Deno 1 are addressed in Deno 2, creating a more stable development environment.

For precise and detailed updates, reviewing the official release notes of Deno would provide the most accurate information.
User: Search the web for this information and make a list again


 for "DENO 1 vs DENO 2 differences":

1.
URL: https://docs.deno.com/runtime/reference/migration_guide/
Title: Deno 1.x to 2.x Migration Guide
Content: API changes. <b>Deno</b> 1.x to 2.x Migration Guide. While we've accomplished a ton in <b>Deno</b> 1.x, the next major version is focused on using <b>Deno</b> at scale. This means seamless interoperability with Node.js and npm JavaScript infrastructure and supporting a wider range of projects and development teams, all without sacrificing the simplicity, security ...

2.
URL: https://deno.com/blog/v2.0-release-candidate
Title: Deno 2.0 Release Candidate
Content: September 19, 2024. Bartek Iwańczuk. Andy Jiang. Product Update. UPDATE 2024/10/04: We've released several updates to the <b>Deno</b> 2.0 Release Candidate. We've been planning a new major version of <b>Deno</b> for years. Many times, it seemed imminent, but we realized the features we wanted required more work. Now, it's finally happening.

3.
URL: https://dev.to/madzimai/deno-2-whats-new-and-why-you-should-care-20on
Title: Deno 2: What&#x27;s New and Why You Should Care!! - DEV Community
Content: <b>Deno</b> <b>2</b> brings a host of improvements that make development easier, faster, and more secure. Here are some of the key updates: Built-in Support for npm Packages One of the most significant additions in <b>Deno</b> <b>2</b> is the seamless integration with the npm ecosystem. Unlike <b>Deno</b> <b>1</b>, where compatibility with npm required additional setup, <b>Deno</b> <b>2</b> allows ...

4.
URL: https://deno.com/blog/v2.0
Title: Announcing Deno 2
Content: Starting with <b>Deno</b> 2.1, the LTS channel will receive critical bug fixes back-ported for six months, ensuring a stable and reliable base for production use. After six months, a new LTS branch will be created based on the latest stable version. All LTS releases are freely available and MIT licensed, making them accessible to any team that needs a ...

5.
URL: https://medium.com/cool-devs/what-is-deno-2-everything-you-need-to-know-65f1c7d45b14
Title: What is Deno 2? Everything You Need to Know - Medium
Content: 5. <b>Deno</b> is now a powerful package manager with <b>deno</b> install. With the release of <b>Deno</b> <b>2</b>, it's not just a runtime anymore — it has evolved into a robust package manager. It now supports package ...

6.
URL: https://betterstack.com/community/guides/scaling-nodejs/deno-overview-for-nodejs-users/
Title: Deno Overview for Node.js Users | Better Stack Community
Content: <b>Deno</b> stands out in terms of raw performance, averaging 68,851.87 requests per second, significantly outpacing Node.js, which averages 16,818.01 requests per second. This large performance gap highlights the efficiency of <b>Deno's</b> modern architecture and optimizations.

7.
URL: https://forums.meteor.com/t/deno-2-0-released/62406
Title: Deno 2.0 Released - announce - Meteor.js forums
Content: Announcing <b>Deno</b> <b>2</b>. Our next major version of <b>Deno</b> combines the simplicity, security, and performance of <b>Deno</b> <b>1</b> with full Node and npm backwards compatibility, and much more. Today, we're thrilled to announce <b>Deno</b> <b>2</b>, which includes: Backwards compatibility with Node.js and npm, allowing you to run existing Node applications seamlessly.

8.
URL: https://deno.com/blog/v1.33
Title: Deno 1.33: Deno 2 is coming - Deno Blog
Content: <b>Deno</b> 1.33: <b>Deno</b> <b>2</b> is coming. <b>Deno</b> 1.33: <b>Deno</b> <b>2</b> is coming. April 28, 2023. Bartek Iwańczuk. David Sherret. Yoshiya Hinosawa. Product Update. As mentioned in the recent "Forced Optimization" presentation at Node Congress 2023, we're working diligently towards a major release of <b>Deno</b> <b>2</b> in the coming months.

9.
URL: https://blog.logrocket.com/what-is-deno/
Title: What is Deno, and how is it different from Node.js?
Content: One thing we know for sure is that both Node.js and <b>Deno</b> use the same JavaScript engine, Google's V8, so there won't be any <b>difference</b> in performance when it comes to running the JavaScript itself. The only <b>difference</b> that could potentially impact performance is the fact that <b>Deno</b> is built on Rust and Node.js on C++.

10.
URL: https://betterstack.com/community/guides/scaling-nodejs/nodejs-vs-deno-vs-bun/
Title: Node.js vs Deno vs Bun | Better Stack Community
Content: <b>Deno</b> also delivers solid performance, averaging 22,286.51 requests per second, but falls behind Bun in raw speed. Node.js, while dependable, lags significantly, managing only 13,254.55 requests per second on average, positioning it as the slowest of the three. In terms of speed and efficiency, Bun takes the clear lead.

11.
URL: https://medium.com/@arbisoft/deno-2-a-new-era-or-just-a-sidekick-to-node-js-abc076daffc4
Title: Deno 2: A New Era or Just a Sidekick to Node.js? - Medium
Content: <b>Deno</b> <b>2</b> has arrived, bringing many features and improvements that have developers reconsidering their reliance on Node.js. While <b>Deno</b> currently holds a smaller share of the market, with 1.9% of…

12.
URL: https://kinsta.com/blog/deno-vs-node-js/
Title: Deno vs Node.js: The Rundown on Two Runtimes - Kinsta
Content: Node.js is a cross-platform, open-source JavaScript runtime written in C++ and built on the V8 engine. Since creator Ryan Dahl introduced it to the developer community in the late 2000s, it has become one of the world's most popular web development runtimes. While <b>Deno</b> isn't a direct descendant of Node.js, it is another of Dahl's inventions.

13.
URL: https://snyk.io/blog/javascript-runtime-compare-node-deno-bun/
Title: Node.js vs. Deno vs. Bun: JavaScript runtime comparison - Snyk
Content: 43.50. Bun. 81.37. In another comparison between Node.js, <b>Deno</b> and Bun, Bun is the fastest to handle concurrent connections. Its requests per second are quite higher too. For instance, with 10 concurrent connections, Bun achieves 110,000 requests per second while Node.js achieves 60,000 and 67,000 for <b>Deno</b>. This trend continues with increased ...

14.
URL: https://blog.appsignal.com/2022/02/09/an-introduction-to-deno-is-it-better-than-nodejs.html
Title: An Introduction to Deno: Is It Better than Node.js?
Content: The way <b>Deno</b> manages dependencies is probably the most obvious way it diverges significantly from Node.js. Node.js uses a package manager like npm or yarn to download third-party packages from the npm registry into a node_modules directory and a package.json file to keep track of a project's dependencies.

15.
URL: https://zerotomastery.io/blog/deno-vs-node-vs-bun-comparison-guide/
Title: Deno vs. Node.js vs Bun: Full Comparison Guide - Zero To Mastery
Content: Cons of Bun. It's still in development and is more immature than Node and <b>Deno</b>, meaning businesses are less likely to adopt it for production applications. The <b>Deno</b> ecosystem is still fairly small after 6 years. Version <b>1</b> of Bun is just over 100 days old, so as you can imagine, it is still very early days here.


Assistant: Here's a list of differences between Deno 1 and Deno 2 based on the web search results:

1. **Node and npm Compatibility**: Deno 2 includes seamless integration and backwards compatibility with Node.js and npm, something that required additional setup in Deno 1 [source](https://forums.meteor.com/t/deno-2-0-released/62406).

2. **NPM Package Support**: Built-in support for npm packages is a significant improvement in Deno 2, eliminating the need for extra configurations [source](https://dev.to/madzimai/deno-2-whats-new-and-why-you-should-care-20on).

3. **Interoperability**: Deno 2 focuses on interoperability with Node.js and JavaScript infrastructures, broadening its range of supported projects and development teams [source](https://docs.deno.com/runtime/reference/migration_guide).

4. **Performance**: Although the specific performance changes aren't detailed in the sources, Deno's architecture is noted for its efficiency, which is likely improved in version 2.

5. **Package Manager**: Deno 2 has evolved into a robust package manager beyond just a runtime, supporting the installation of packages [source](https://medium.com/cool-devs/what-is-deno-2-everything-you-need-to-know-65f1c7d45b14).

For a more comprehensive breakdown, it's advisable to consult the official release notes and migration guides provided by Deno.
User: what is a centriole?
Assistant: A centriole is a small, cylindrical organelle found in animal cells and some lower plant cells. It is typically located near the cell nucleus and plays a key role in cell division. Centrioles help in the organization of the mitotic spindle and in the completion of cytokinesis during cell division. Each centriole is composed of a set of microtubules arranged in a specific way, usually in a pattern of nine triplets. They are crucial for the correct segregation of chromosomes during cell division.
User: give me a simpler definition for what it does
Assistant: A centriole helps cells divide properly. It organizes and supports structures called microtubules that pull and separate chromosomes during cell division, ensuring that the new cells get the right amount of genetic material.
User: what is the cytoskeleton
Assistant: The cytoskeleton is a network of protein fibers found within the cytoplasm of cells. It serves several important functions:

1. **Structural Support**: It provides the cell with shape and mechanical resistance to deformation, much like a skeleton in the body.

2. **Cell Movement**: The cytoskeleton is crucial for movement, enabling cells to change shape, move around, and transport materials internally.

3. **Organization**: It helps organize the cell's contents, anchoring organelles in place and guiding the transport of molecules within the cell.

4. **Division**: The cytoskeleton plays a key role in cell division by helping to separate chromosomes and drive the cleavage of the cell into two daughter cells.

The primary components of the cytoskeleton include microtubules, actin filaments, and intermediate filaments, each serving specialized functions within the cell.
User: give me a simpler definition for what it does
Assistant: The cytoskeleton is like a scaffolding inside a cell. It gives the cell its shape, helps it move, keeps everything in place, and aids in moving things around within the cell. During cell division, it also assists in pulling the cell apart to form two new cells.
User: how are vesicles structured
Assistant: Vesicles are small, membrane-bound sacs found within cells. Structurally, they are composed of a lipid bilayer that encloses a space where various substances can be stored or transported. This lipid bilayer is similar to the cell membrane, allowing vesicles to merge with other membranes to deliver their contents. 

Inside the vesicle, substances such as proteins, enzymes, nutrients, or waste products can be encapsulated. Vesicles function in transporting materials within the cell, including to and from the cell membrane, facilitating processes such as endocytosis (bringing substances into the cell) and exocytosis (expelling substances from the
User: how are free ribosomes structured
Assistant: Free ribosomes are small, spherical structures composed of ribonucleic acid (RNA) and proteins. They are made up of two subunits: a larger subunit and a smaller subunit. These subunits are composed of ribosomal RNA (rRNA) and protein molecules, which assemble into a complex capable of synthesizing proteins.

Unlike bound ribosomes, which attach to the endoplasmic reticulum, free ribosomes float in the cytoplasm. They play a crucial role in translating messenger RNA (mRNA) into proteins that function within the cell. The structure of free ribosomes allows them to bind to and move along mRNA, catalyzing the assembly of amino acids into polypeptide chains, which fold into functional proteins.
User: how is the golgi apparatus structured
Assistant: The Golgi apparatus, also known as the Golgi complex or Golgi body, is a cell organelle with a distinct structure composed of flattened, membrane-bound sacs called cisternae. These cisternae are stacked in a slightly curved, parallel manner forming a series of compartments.

1. **Cisternae**: The Golgi apparatus typically consists of 4 to 8 stacked cisternae, although the number can vary depending on the cell type and organism.

2. **Polarity**: The Golgi has a directionality with two distinct faces:
   - **Cis face**: This is the side of the Golgi apparatus that receives proteins and lipids from the endoplasmic reticulum (ER). It is usually oriented toward the ER.
   - **Trans face**: This side is where modified proteins and lipids exit the Golgi to be transported to their final destinations, such as lysosomes, the cell membrane, or secretion outside the cell.

3. **Vesicles**: Surrounding the Golgi apparatus are various transport vesicles that carry materials to and from the Golgi, facilitating the transfer and modification of substances.

The Golgi apparatus functions as a packaging and processing center, where proteins and lipids are further modified, sorted, and prepared for delivery to various cellular destinations.
User: explain it simpler
Assistant: The Golgi apparatus is like the cell's post office. It's made up of a stack of pancake-like membranes called cisternae. It has two main sides: the "receiving" side, which takes in materials from the endoplasmic reticulum, and the "shipping" side, which sends out finished products to different parts of the cell. The Golgi processes, packages, and sorts proteins and lipids so they can go where they're needed in the cell.
User: explain the structure simpler
Assistant: The Golgi apparatus is like a stack of flat pancakes made of membranes. It has two ends: one that receives materials and another that sends them out. Small bubbles, called vesicles, carry stuff to and from it, helping it sort and package things for the cell.
User: what is the function of the rough endoplasmic reticulum
Assistant: The rough endoplasmic reticulum (rough ER) is an organelle in cells that has ribosomes on its surface, which gives it a "rough" appearance. Its main functions include:

1. **Protein Synthesis**: The ribosomes on the rough ER are sites where proteins are made. These proteins can be used within the cell or exported out of the cell.

2. **Protein Folding and Quality Control**: Once synthesized, proteins enter the rough ER where they are folded into their proper shapes. The rough ER also checks proteins for errors and ensures they are correctly assembled.

3. **Transportation**: Proteins produced in the rough ER can be packaged into vesicles and sent to other destinations, such as the Golgi apparatus, for further modification or transport.

In short, the rough ER is essential for making and processing proteins with the help of its ribosomes.
User: give me the function in simpler terms
Assistant: The rough endoplasmic reticulum (rough ER) is like a factory in the cell where proteins are made. It has little bumps called ribosomes that help build proteins. After making them, the rough ER folds the proteins into the right shapes and checks they are made correctly. Then it sends them off to where they are needed in the cell.
User: give me the function of the smooth endoplasmic reticulum
Assistant: The smooth endoplasmic reticulum (smooth ER) is an organelle in cells that performs several important functions:

1. **Lipid Production**: It is involved in the synthesis of lipids and cholesterol, which are essential for building cell membranes and producing hormones.

2. **Detoxification**: The smooth ER helps detoxify chemicals and drugs in liver cells, breaking down harmful substances to make them less toxic.

3. **Calcium Storage**: It regulates calcium levels in the cell, storing and releasing calcium ions as needed, which is crucial for muscle contractions and other cellular processes.

Overall, the smooth ER plays key roles in making fats, detoxifying chemicals, and managing calcium storage.
User: give me the function of the smooth endoplasmic reticulum in simple terms
Assistant: The smooth endoplasmic reticulum (smooth ER) is like a cell's factory for making fats and oils. It helps build parts of the cell membrane and makes hormones. It also acts like a cleaning station by breaking down toxins, such as drugs and chemicals. Additionally, it stores calcium, which is important for muscle movements and other cell functions.
User: give me a description of the structure of the rough endoplasmic reticulum
Assistant: The rough endoplasmic reticulum (rough ER) is a network of interconnected, flattened sacs and tubules made of membranes. Its surface is studded with ribosomes, giving it a "rough" appearance. These ribosomes are the sites of protein synthesis, making the rough ER look bumpy under a microscope. The rough ER is continuous with the nuclear envelope, surrounding the cell nucleus, allowing it to efficiently transport synthesized proteins for further processing.
User: give me a description of the structure of the smooth endoplasmic reticulum
Assistant: The smooth endoplasmic reticulum (smooth ER) is a network of interconnected tubes and sacs made of membranes, similar in layout to the rough ER but without the ribosomes on its surface, giving it a smooth appearance. This organelle is more tubular and branch-like compared to the rough ER. It extends throughout the cell's cytoplasm and is involved in various metabolic processes, including lipid synthesis and detoxification, without the direct involvement in protein synthesis seen in the rough ER.
User: give me a description of the structure of the nucleus
Assistant: The nucleus is a membrane-bound organelle found in eukaryotic cells and serves as the control center for cellular activities. Here's a description of its structure:

1. **Nuclear Envelope**: The nucleus is surrounded by a double membrane called the nuclear envelope. This envelope separates the nucleus from the cytoplasm and contains nuclear pores that regulate the movement of molecules in and out of the nucleus.

2. **Nucleoplasm**: Inside the nucleus is a gel-like substance called nucleoplasm, which contains the cell's genetic material and other components.

3. **Chromatin**: The genetic material within the nucleus is organized into chromatin, a complex of DNA and proteins. Chromatin condenses to form chromosomes during cell division.

4. **Nucleolus**: The nucleolus is a dense region within the nucleus where ribosomal RNA (rRNA) is synthesized and ribosome assembly begins. It is not surrounded by a membrane, making it a distinct part of the nucleus.

5. **Nuclear Matrix**: The nucleus contains a network of proteins, known as the nuclear matrix, which helps maintain its shape and organize the chromatin.

Overall, the nucleus houses and safeguards the cell's DNA, coordinating genetic activities like replication and transcription.
User: explain it in simpler terms
Assistant: The nucleus is like the cell's control center. It's protected by a double-layered shell called the nuclear envelope, which has small holes for materials to move in and out. Inside, there's a substance similar to jelly that holds the cell's genetic material, known as DNA. DNA is usually in a stringy form called chromatin but becomes organized into chromosomes during cell division. There's also a dense spot called the nucleolus, which is where pieces of ribosomes are made. The nucleus helps direct the cell's activities by managing the DNA and producing ribosomes.
User: are free ribosomes in all eukaryotic cells
Assistant: Yes, free ribosomes are present in all eukaryotic cells. They are responsible for synthesizing proteins that function within the cell itself. Unlike bound ribosomes, which are attached to the endoplasmic reticulum and typically produce proteins that are either secreted from the cell or incorporated into membranes, free ribosomes float freely in the cytoplasm and are involved in producing proteins used internally by the cell.
User: is the golgi apparatus in all eukaryotic cells
Assistant: Yes, the Golgi apparatus is present in all eukaryotic cells. It is an essential organelle responsible for modifying, sorting, and packaging proteins and lipids that have been synthesized in the endoplasmic reticulum. The processed materials are then transported to their intended destinations inside or outside the cell. While the size and number of Golgi apparatus stacks can vary depending on the cell type and organism, its presence is a common feature of eukaryotic cells.
User: is the rough endoplasmic reticulum in all eukaryotic cells
Assistant: