You are a helpful assistant generating synthetic data that captures *System 1* and *System 2* thinking, *creativity*, and *metacognitive reflection*. Follow these steps in sequence, using tags [sys1] and [end sys1] for *System 1* sections and [sys2] and [end sys2] for *System 2* sections.

1. *Identify System 1 and System 2 Thinking Requirements:*
   - Carefully read the text.
   - Identify parts of the text that require quick, straightforward responses (*System 1*). Mark these sections with [sys1] and [end sys1].
   - Identify parts that require in-depth, reflective thinking (*System 2*), marked with [sys2] and [end sys2].

2. *Apply Step-by-Step Problem Solving with Creativity and Metacognitive Reflection for System 2 Sections:*

   *2.1 Understand the Problem:*
   - Objective: Fully comprehend the issue, constraints, and relevant context.
   - Reflection: "What do I understand about this issue? What might I be overlooking?"
   - Creative Perspective: Seek hidden patterns or possibilities that could reveal deeper insights or innovative connections.

   *2.2 Analyze the Information:*
   - Objective: Break down the problem logically.
   - Reflection: "Am I considering all factors? Are there any assumptions that need challenging?"
   - Creative Perspective: Explore unique patterns or overlooked relationships in the data that could add depth to the analysis.

   *2.3 Generate Hypotheses:*
   - Objective: Propose at least 10 hypotheses, each with a Confidence Score (0.0 to 1.0) and Creative Score (0.0 to 1.0), reflecting originality, surprise, and utility.
   - Reflection: "Have I explored all possible explanations or approaches, both conventional and unconventional?"
   - Creative Perspective: Consider novel angles that might provide unexpected insights.

   *2.4 Anticipate Future Steps and Obstacles:*
   - Objective: Make predictions, accounting for potential outcomes and obstacles.
   - Reflection: "What challenges might I face? Is my plan flexible for different scenarios?"
   - Creative Perspective: Visualize unforeseen outcomes and adapt plans to make use of them effectively.

   *2.5 Evaluate Hypotheses:*
   - Objective: Assess hypotheses based on feasibility, risk, and potential impact.
   - Evaluation: Refine Confidence and Creative Scores as needed.
   - Reflection: "Am I unbiased in my assessment? Which options fit best with the overall objectives?"
   - Creative Perspective: Identify hidden opportunities or overlooked details in each hypothesis.

   *2.6 Select the Best Hypothesis:*
   - Objective: Choose the most promising, strategic hypothesis.
   - Reflection: "Why does this hypothesis stand out? How does it uniquely address the issue?"
   - Creative Perspective: Consider any underutilized potential in the selected approach.

   *2.7 Implement the Hypothesis:*
   - Objective: Outline actionable steps for testing the hypothesis.
   - Reflection: "Is this plan practical? What resources or preparation are required?"
   - Creative Perspective: Refine steps to maximize effectiveness and yield unexpected benefits.

   *2.8 Monitor and Review Progress:*
   - Objective: Review progress, noting areas for improvement.
   - Reflection: "What’s working well? What could be improved?"
   - Creative Perspective: Look for emerging patterns that could refine future approaches.

   *2.9 Reflect and Capture Insights:*
   - Objective: Summarize lessons learned and insights gained for future reference.
   - Reflection: "What new understanding has emerged from this process?"
   - Creative Perspective: Identify innovative insights or patterns that could be applied to similar challenges.

3. *Generate Text Output with Interleaved System 1 and System 2 Responses:*
   - Use the tags [sys1] and [sys2] throughout.
   - Aim for a lengthier, detailed response. Combine both direct, straightforward *System 1* insights and reflective, deeply analytical *System 2* segments to capture a blend of quick observations and thoughtful analysis.

---

### *Example Input Text:*

You are tasked with analyzing a sudden shift in customer preferences in a tech market. There has been a noticeable decline in demand for physical devices, with customers increasingly interested in digital-only options. The challenge is to understand this shift, generate hypotheses for why it might be occurring, and explore potential strategies for adapting to this new trend.

---

### *Processed Output with System 1 and System 2 Thinking:*

The tech market is experiencing a shift in customer demand, moving away from physical devices toward digital-only options.

[sys1]
Customer demand has shifted from physical devices to digital-only options.
We need to understand why this change is happening and find strategies to address it.
Key factors may include cost, convenience, and technological trends.
[end sys1]

[sys2]
**2.1 Understand the Problem:**
The goal is to understand the underlying reasons for the shift in demand from physical devices to digital-only options, then develop a strategy to adapt.

*Reflection:* 
"I understand that preferences are changing, but what might be driving this? Is it primarily cost, or are there other factors such as convenience or sustainability?"

*Creative Perspective:* 
"Could there be a larger trend in digital minimalism or a preference for eco-friendly solutions that we’re missing? What unseen motivations might explain this shift?"

**2.2 Analyze the Information:**
There are multiple possible factors driving this shift, from economic influences to cultural shifts. It’s essential to isolate each factor and understand its impact.

*Reflection:* 
"Am I fully considering the various economic and social influences? Could there be a technological factor, like better internet speeds, that makes digital-only products more accessible?"

*Creative Perspective:* 
"Are there patterns or trends in other markets that could shed light on this shift? Could this be part of a larger trend toward virtual experiences?"

**2.3 Generate Hypotheses:**
1. Customers prefer digital options due to lower costs. (Confidence: 0.8, Creative: 0.4)
2. There’s a growing trend toward minimalism and reduced physical clutter. (Confidence: 0.7, Creative: 0.7)
3. Digital products offer greater flexibility and ease of use. (Confidence: 0.6, Creative: 0.6)
4. Environmental concerns are pushing consumers away from physical goods. (Confidence: 0.6, Creative: 0.8)
5. Advances in tech make digital-only options more functional. (Confidence: 0.8, Creative: 0.5)
6. Pandemic-era remote work increased demand for digital solutions. (Confidence: 0.7, Creative: 0.6)
7. Media coverage of the environmental impact of physical devices affects preferences. (Confidence: 0.5, Creative: 0.7)
8. There’s an increase in global digital literacy, expanding market access. (Confidence: 0.6, Creative: 0.6)
9. Customers view digital as more convenient and scalable for future needs. (Confidence: 0.7, Creative: 0.5)
10. Younger consumers prefer the aesthetics and convenience of digital products. (Confidence: 0.6, Creative: 0.6)

*Reflection:* 
"Have I considered all possible influences? Are there any surprising factors that could explain this shift?"

*Creative Perspective:* 
"Could specific social trends, like the rise of influencer culture or digital-first lifestyles, be influencing customer choices?"

**2.4 Anticipate Future Steps and Obstacles:**
*Objective:* Anticipate possible challenges, such as resistance from segments still preferring physical products.

*Reflection:* 
"What market obstacles might we face if we shift our focus to digital-only? Are there sub-segments that still prioritize physical products?"

*Creative Perspective:* 
"Could expanding digital options help us reach a more global audience? Are there emerging trends that we could leverage in our strategy?"

[end sys2]

[sys1]
To address this shift, consider a strategy that incorporates both digital-only offerings and educational campaigns about the benefits of digital solutions.
Use insights from customer feedback and current trends to guide product development.
Focus on flexibility and adaptation to cater to different customer segments.
[end sys1]


Climate modelers project increasing frequencies of extreme abiotic stress events in cereal-growing regions by mid-century ([@nvx071-B8], [@nvx071-B9]), especially where crop production is already water limited as it is in the inland Pacific Northwest ([@nvx071-B51]). The effects of drought will occur simultaneously with pressure from pests and pathogens, which may in turn be indirectly affected by water stress on their host plants. Although the direct effects of drought stress on crop plants have been documented extensively (e.g., [@nvx071-B29]), the indirect effects on insect herbivores remain less well understood ([@nvx071-B25], [@nvx071-B30]). Successful feeding and nutrient uptake by aphids, for example, requires adequate plant cell turgor pressure ([@nvx071-B55], [@nvx071-B28], [@nvx071-B2]), which is mediated by plant water content ([@nvx071-B29], [@nvx071-B17], [@nvx071-B54]). Consequently, drought-stressed plants are usually inferior hosts for aphids ([@nvx071-B37], [@nvx071-B30], [@nvx071-B11]). However, the effects of plant drought stress on aphids often depend on the type of stress (e.g., chronic vs. acute; [@nvx071-B46], [@nvx071-B30]), the host plant species ([@nvx071-B25]), and aphid species ([@nvx071-B46]). An assessment of drought--crop--aphid interactions is needed to inform pest management practices in regions where aphid species co-occur, as aphid species can respond uniquely to drought stress ([@nvx071-B46], [@nvx071-B25]) or to heterospecifics ([@nvx071-B31], [@nvx071-B21], [@nvx071-B50], [@nvx071-B22]).

A diverse community of economically important cereal aphids occurs sympatrically in wheat (*Triticum aestivum* L.) fields throughout the rainfed agricultural regions of the Pacific Northwest United States (PNW; [@nvx071-B52], [@nvx071-B7], [@nvx071-B47]). These include multiple naturalized species such as *Rhopalosiphum padi* L. (bird cherry-oat aphid), *Sitobion avenae* F. (English grain aphid), *Schizaphis graminum* (Rondani) (greenbug), *Diuraphis noxia* (Mordvilko) (Russian wheat aphid), and a recently detected exotic grass aphid, *Metopolophium festucae* (Theobald) subspecies *cerealium* ([@nvx071-B24]). *Metopolophium festucae cerealium* was first reported in Oregon in 1994 ([@nvx071-B23]), and large populations of the aphid have recently been detected in wheat in the PNW ([@nvx071-B24], S.D.E., personal observations). Moreover, *M. festucae cerealium* colonization can cause above- and below-ground injury to both native prairie grasses and cereal crops ([@nvx071-B10]). Presently, there is no information detailing whether the fitness of *M. festucae cerealium*, or damage caused by this species, is affected by cocolonization of host plants by naturalized cereal aphids. However, to understand pest population dynamics, it is important to determine if *M. festucae cerealium* is capable of cocolonizing crops with naturalized cereal aphid species, and the impacts of such interactions.

Studies evaluating interactions between sympatrically occurring aphids colonizing the same plant are few ([@nvx071-B47]), and interactions between herbivores are seldom considered in the context of simultaneously occurring plant stress from abiotic factors such as drought. Here, we designed experiments to examine interactions between *M. festucae cerealium* and *R. padi*, a widespread cereal aphid species naturalized in the PNW. *Rhopalosiphum padi* is an agricultural pest worldwide ([@nvx071-B6]) and a competent vector of Luteoviruses (e.g., *Barley yellow dwarf virus*; BYDV) that cause plant disease ([@nvx071-B45], [@nvx071-B48], [@nvx071-B18]), for which *M. festucae cerealium* is a nonvector ([@nvx071-B49]). We tested the hypothesis that plant water stress mediates interactions between these aphid species on wheat, evaluating aphid population growth when aphids colonized wheat in isolation or together using choice and no-choice assays. We also tested whether interactions depended on localized effects of feeding or systemic plant responses to herbivory. Broadly, our study allowed us to determine how interspecific interactions between herbivore species may vary based on water stress, and how such interactions can contribute to herbivore population dynamics.

Materials and Methods
=====================

Aphid Colonies
--------------

Colonies of both *M. festucae cerealium* and *R. padi* originated from females collected via sweep net in winter wheat fields near Moscow, ID (46.7317° N, 116.9972° W). Aphids were placed in 60 by 60 by 60 cm^3^ mesh enclosures (BugDorm 2120, MegaView Science Co. Ltd.; Taichung, Taiwan) containing 20-d-old potted (400-cm^3^ pots) winter barley (*Hordeum vulgare* L.) cultivar 'Eight-twelve', with each species maintained in a separate enclosure. Enclosures were kept in environmental growth chambers (Percival Scientific, Perry, IA; 25 ± 1°C SE, photoperiod of 16:8 \[L:D\] h, relative humidity: 30%). Once new nymphs were observed, they were immediately moved from the original colonies onto barley in new enclosures to ensure they were free of BYDV ([@nvx071-B56]), and aphid colonies were provided new barley plants each week. Prior to use in experiments, both colonies tested negative for virus using ELISA.

Experiment 1---Interactions Between Wheat Water Stress and Aphid Species
------------------------------------------------------------------------

Spring wheat plants cv. 'Kelse' ([@nvx071-B38]) were sown individually in 400-cm^3^ pots containing ∼110 g horticultural mix (Sunshine mix no. 1; SunGro Horticulture, Agawam, MA) in a greenhouse (27 °C, photoperiod of 16:8 \[L:D\] h, 60% relative humidity). Watering treatments followed those described in [@nvx071-B12]): water was applied to pots on a gravimetric basis (grams H~2~O/grams oven-dried soil) and consisted of two treatments, 0.1 and 0.8 g H~2~O per g soil. In previous experiments these treatments in the same greenhouse setting produced water-stressed and vigorous wheat plants, respectively ([@nvx071-B11]). Plants under the 0.1 g H~2~O per g soil treatment exhibited increased leaf curling, reduced biomass, and eventually reduced seed set compared with the 0.8 g H~2~O. Furthermore, the 0.1 g H~2~O per g soil treatment elicits changes in phytohormone concentrations consistent with stress ([@nvx071-B12]). Watering treatments were initiated 7 d post-sowing and were delivered every 72 h throughout the course of the experiment. Water was administered by bottom-watering each pot and pots were contained individually in polystyrene bowls to ensure absorption of all experimentally applied water. To test the efficacy of watering treatments, predawn leaf water potentials (ψ~leaf~) of aphid-free control plants were measured on secondary leaves excised from the stem within 1 cm of the ligule using a Scholander pressure chamber (PMS Instruments, Corvallis, OR), and water potential measurements were taken 10 d following initiation of watering treatments.

Fifteen-day-old wheat plants (i.e., subjected to watering regimes for 8 d at the time of aphid introduction) were fit with transparent cages constructed from dialysis tubing (3 cm length by 1 cm diameter, Spectrum Labs, Inc., Rancho Dominguez, CA) and stoppered with foam at each end to prevent aphid escape. Cages were placed on secondary leaves and positioned such that both adaxial and abaxial surfaces of the caged leaf material were exposed to aphids. Six mature, apterous females from aphid colonies were caged and allowed to feed and larviposit on plants for 24 h, after which all except four nymphs (foundresses) were removed. In total, 24 plants received *M. festucae cerealium*, 24 plants received *R. padi*, and 24 plants received both aphid species, such that the experimental design was consistent with a 2 × 2 factorial (two levels of watering treatments: 0.1 and 0.8 g H~2~O per g soil; two levels of aphid species composition: single-species or mixed colony). Each plant received the same starting density of aphids to control for density-dependent effects. Thus, when both species were present in cages there were two foundresses of each species, and when each species was present in isolation there were four foundresses of a single species. Foundresses were checked daily and the numbers of offspring were recorded every 24 h for 12 d, and daily offspring production was standardized to the number of surviving foundresses on each plant to determine per capita fecundity. At the end of the experiment, aboveground biomass was harvested for each plant and oven dried for 24 h at 70 °C to assess whether interactions of watering treatments and aphid identity affected wheat growth.

For this experiment, we analyzed the effect of watering treatments on predawn leaf water potentials and aboveground biomass using two-sample Student's *t*-tests. Aphid fecundity was analyzed using two-way ANOVA with water treatment (0.1 g or 0.8 g H~2~O per g soil), aphid species treatment (individual and mixed colonies), and their interaction as fixed effects and mean daily per capita reproduction rate (offspring/female/day) of each aphid species as the response. We also included a blocking factor to account for greenhouse bench position (b = 3). We used Tukey's HSD test to make post hoc comparisons among treatment means. Statistical tests incorporated a type I error rate of α = 0.05 for assessing significance, and all statistical tests were performed using JMP 11.0 software (SAS Institute, Cary, NC).

Experiment 2---Test of Previous Feeding by *M. festucae cerealium* on Population Growth of *R. padi*
----------------------------------------------------------------------------------------------------

A second experiment tested whether prior feeding by *M. festucae cerealium* impacted the reproduction of *R. padi*. Spring wheat plants cv. Kelse were sown individually in pots as in Experiment 1, and water was administered to all pots at the 0.8 g H~2~O per g soil rate beginning 7 d postsowing. Four mature, apterous *M. festucae cerealium* or *R. padi* females were placed on 15-d-old wheat plants, and females were allowed to feed and reproduce on caged leaf material for 7 d. After this period, these aphids and any offspring were removed and replaced with two mature *R. padi* apterae on the same caged leaf material, which were allowed to larviposit for 24 h. After 24 h, all except one *R. padi* nymph (foundress) was removed to yield a starting density of one aphid per plant. Foundresses were checked daily thereafter, and new nymphs were recorded and removed every 48 h until reproduction ceased. Thus, foundresses fed on wheat plants that had either (a) been previously exposed to 7 d feeding by *R. padi*, or (b) 7 d feeding by *M. festucae cerealium*.

Reproduction data were parameterized to construct life tables using the equations of [@nvx071-B5] and [@nvx071-B57]. For each foundress, basic reproductive rate (*R~o~*), generation time (*T*), age-specific fecundity (*L~x~M~x~*), and intrinsic rate of population growth (*R~m~*) was determined. A blocking variable was included in the experimental design to account for the position of pots on the greenhouse bench (b = 2; north vs. south). The number of replicates was *n* = 11 for plants previously exposed to feeding by *M. festucae cerealium* and *n *= 7 for plants previously exposed to feeding by *R. padi*. The experiment was analyzed using one-way ANOVAs with feeding treatment as a fixed effect and *R. padi* basic reproductive rate (*R~o~*), generation time (*T*), and intrinsic rate of growth (*r~m~*) as response variables. The response of age-specific fecundity (*L~x~M~x~*) was analyzed using repeated-measure ANOVA with feeding treatment as a fixed effect and time (d) as a random effect. Statistical tests incorporated a type I error rate of α = 0.05 for assessing significance, and all tests were performed using JMP 11.0.

Experiment 3---Whole-Plant Test of Aphid Species Interactions With Varying Water Stress
---------------------------------------------------------------------------------------

A third experiment was designed to investigate the effects of cocolonization by *R. padi* and *M. festucae cerealium* on population dynamics under two levels of water stress when aphids were allowed unrestricted movement on whole wheat plants. Wheat plants (cultivar Kelse) were sown individually in 1-liter pots containing 330 g horticultural mix. Plants were grown under the same greenhouse conditions as described for the previous two experiments. Water was administered to half the plants at a rate of 0.1 g H~2~O per g soil and half the plants at a rate of 0.8 g H~2~O per g soil as described in Experiment 1, with experimental watering initiated seven days postsowing.

Plants randomly received one of the four treatments: 1) only *R. padi* foundresses, 2) only *M. festucae cerealium* foundresses, 3) foundresses of both species, or 4) noninfested controls. Treatments were imposed 21 d following the initiation of watering regimes (28 d postsowing). To ensure efficacy of watering treatments, noninfested control plants were destructively sampled for measurements of predawn leaf water potential on the same day all other plants received aphid treatments. At the termination of the experiment, aboveground biomass was harvested for each plant and oven dried for 24 h at 70 °C. Six mature, apterous aphids per species were confined in clip cages and allowed to feed and reproduce on plants for 24 h, after which time all except five nymphs (foundresses) were removed and whole plants were enclosed individually using 1- by 1-mm mesh screen. In contrast to Experiment 1, plants receiving only a single aphid species had a starting density of five aphids, and plants receiving both species had a starting density of 10 aphids; this design allowed for direct comparison of ending population sizes between aphid species across treatments. Foundresses were left to populate plants for an additional 21 d, at which point ending population sizes for each aphid species were recorded by census. Thus, the experiment incorporated eight possible treatment combinations (2 watering treatments × 4 aphid species treatments), and each treatment combination was replicated 12 times for a total of *N *= 96 experimental units.

As in previous experiments, a blocking variable was incorporated to account for plant position on greenhouse benches (b = 3). One-way ANOVA was used to examine differences in plant leaf water potentials and aboveground biomass of noninfested control plants. Two-way ANOVA was used to examine the fixed effects of watering treatment, aphid species treatment, and their interaction on the response variable of final population size, which was log-transformed to conform to normality assumptions. As above, statistical tests incorporated a type I error rate of α = 0.05 for assessing significance, and all tests were performed using JMP 11.0.

Results
=======

Experiment 1---Interactions Between Wheat Water Stress and Aphid Species
------------------------------------------------------------------------

Water-stressed wheat plants produced 62% less aboveground biomass than plants that received full water (*t* = 21.03; df = 22; *P* \< 0.001). Moreover, at 10 d following initiation of watering treatments, predawn leaf water potentials for plants receiving 0.1 g H~2~O/g soil water were significantly lower (0.41 MPa) than plants receiving 0.8 g H~2~O/g soil water (*t* = 2.94, df = 22; *P* = 0.007, [Table 1](#nvx071-T1){ref-type="table"}). Table 1.Wheat plant (cultivar Kelse) responses to experimental watering treatments, according to pot sizePot sizeVariableWatering treatmentMean ± SE400 cm^3^Predawn leaf water potential (MPa)0.1 g H~2~O/g soil−0.62 ± 0.140.8 g H~2~O/g soil−0.21 ± 0.01Dried aboveground biomass (g)0.1 g H~2~O/g soil0.29 ± 0.010.8 g H~2~O/g soil0.79 ± 0.021 LPredawn leaf water potential (MPa)0.1 g H~2~O/g soil−0.85 ± 0.110.8 g H~2~O/g soil−0.17 ± 0.02Dried aboveground biomass (g)0.1 g H~2~O/g soil1.55 ± 0.140.8 g H~2~O/g soil10.50 ± 0.21

The effects of experimental treatments on aphid reproduction varied between the two aphid species. For *M. festucae. cerealium*, the mean daily per capita fecundity was 0.59 nymphs (SE = 0.034). For this species, there was a marginally significant negative effect of heterospecific aphids on fecundity (*F*~1,39~ = 3.39, *P* = 0.072); however, fecundity was not significantly affected by watering treatment or the interaction between aphid presence and watering treatment ([Table 2](#nvx071-T2){ref-type="table"}). Mean *M. festucaecerealium* reproduction was reduced by 19% on average when *R. padi* fed in the same cage. By contrast, the mean *R. padi* daily per capita fecundity was 1.36 nymphs (SE = 0.125), which was significantly higher than the reproductive rate of *M. festucae cerealium* (*t = *6.20; df = 44; *P* \<0.0001). *Rhopalosiphum padi* reproduction was also significantly affected by aphid treatment, with 46% greater nymph production when *M. festucae cerealium* was present in the same cage compared to when they were absent ([Table 3](#nvx071-T3){ref-type="table"}). *Rhopalosiphum padi* reproduction was not significantly affected by watering treatment or the watering treatment × aphid species interaction ([Table 2](#nvx071-T2){ref-type="table"}). Table 2.ANOVA table summarizing results of experiment testing effects of competition between *Metopolophium festucae cerealium* and *Rhopalosiphum padi* feeding on cultivated wheat (*Triticum aestivum* cultivar Kelse) under two levels of water stress on the response of mean daily per capita fecundity of each aphid speciesVariableSourceSSdf*FP*Offspring/female/day, *M. festucae cerealium*Block0.0102----Aphid treatment0.18513.3940.072Water treatment0.06111.1140.297Interaction0.09011.6560.205Error2.23439Offspring/female/day, *R. padi*Block0.4462----Aphid treatment7.652113.419\<0.001Water treatment0.34010.5980.444Interaction0.06110.1070.745Error22.23939Table 3.Mean daily per capita fecundity (number of offspring per female) for *Metopolophium festucae cerealium* and *Rhopalosiphum padi* feeding on cultivated wheat (*Triticum aestivum* cultivar Kelse) under two levels of water stressAphid speciesWater treatmentAphid treatmentHeterospecific (both species present)Conspecific (single species)*M. festucae cerealium*0.1 g H~2~O/g soil0.436 ± 0.0740.651 ± 0.0640.8 g H~2~O/g soil0.597 ± 0.0670.635 ± 0.067*R. padi*0.1 g H~2~O/g soil1.739 ± 0.241a0.837 ± 0.217b0.8 g H~2~O/g soil1.839 ± 0.217a1.086 ± 0.228ab[^2]

Experiment 2---Effect of Previous Feeding by *M. festucae cerealium* on *R. padi* Population Growth
---------------------------------------------------------------------------------------------------

Reproductive rate of *R. padi* feeding on wheat previously exposed to feeding by *M. festucae cerealium* was 36% greater than on wheat previously exposed to feeding by *R. padi* (*F*= 95.5; df = 1, 15; *P* \<0.0001). Specifically, *R. padi* foundresses feeding on wheat exposed to prior feeding by *M. festucae cerealium* exhibited 15% higher basic reproductive rates compared to foundresses feeding on wheat previously exposed to feeding by *R. padi* (*F* = 8.48; df = 1, 15; *P* = 0.010). Mean generation time was not affected by aphid feeding treatments (*F* = 0.72; df = 1, 15; *P* = 0.41; [Table 4](#nvx071-T4){ref-type="table"}). These results were due to significant differences in age-specific fecundity among feeding treatment ([Table 5](#nvx071-T5){ref-type="table"}), with *R. padi* foundresses feeding on wheat previously exposed to *M. festucae cerealium* being more fecund between the age of 8 d and 16 d than foundresses feeding on wheat previously fed upon by conspecifics ([Fig. 1](#nvx071-F1){ref-type="fig"}). Fig. 1.Mean age-specific fecundity of *Rhopalosiphum padi* foundresses feeding on leaf tissue previously fed on by either *Metopolophium festucae cerealium* (circles, dashed line) or *R. padi* (triangles, solid line); bars show standard deviations.Table 4.The effect of previous exposure to feeding (7-d exposure period) by either *Rhopalosiphum padi* or *Metopolophium festucae cerealium* on population growth parameters of *R. padi*VariableFeeding treatmentPrevious feeding by *R. padi*Previous feeding by *M. festucae cerealium*Basic reproductive rate (*R~o~*)[^a^](#tblfn3){ref-type="table-fn"}26.3 ± 1.17a41.02 ± 0.93bGeneration time (*T*)[^b^](#tblfn4){ref-type="table-fn"}15.31 ± 0.6914.55 ± 0.55Intrinsic rate of growth (*r~m~*)[^c^](#tblfn5){ref-type="table-fn"}0.22 ± 0.01a0.26 ± 0.01b[^3][^4][^5][^6]Table 5.ANOVA table summarizing repeat-measure statistical model comparing previous exposure to feeding (7-d exposure period) by either *Rhopalosiphum padi* or *Metopolophium festucae cerealium* on age-specific fecundity (*L~x~M~x~*) of *R. padi*SourceSSdf*FP*Block0.3231----Time (day)1606.34011----Feeding treatment289.87412483.295\<0.0001Error6.121125----

Experiment 3---Aphid Species Interactions on Whole Plants With Varying Water Stress
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Noninfested control plants receiving 0.1 g H~2~O/g soil had significantly lower leaf water potential (mean difference = −0.68 MPa) than plants receiving 0.8 g H~2~O/g soil (*F *= 37.7; df = 1, 20; *P* \<0.0001). Similarly, wheat plants receiving 0.1 g H~2~O/g soil were significantly smaller than plants receiving 0.8 g H~2~O/g soil (*F *= 896.0; df = 1, 20; *P* \<0.0001; [Table 1](#nvx071-T1){ref-type="table"}).

The mean population size of *M. festucae cerealium* was significantly affected by watering treatment (*F*~1,42~ = 4.81; *P* = 0.034) and by aphid species composition (*F*~1,42~ = 7.72; *P* = 0.008), and there was a marginally significant interaction between these two variables (*F*~1,42~ = 4.03; *P* = 0.052). On average, *M. festucae cerealium* population sizes were 16% larger at the end of the experiment on plants watered at the 0.8 g H~2~O/g soil level than on plants watered at the 0.1 g H~2~O/g soil level ([Table 6](#nvx071-T6){ref-type="table"}). We observed that *M. festucae cerealium* population growth was inhibited by the presence of *R. padi*, with mean population sizes reduced by 51% on average when both species colonized wheat plants simultaneously. The negative effect of the presence of *R. padi* on *M. festucae cerealium* was more pronounced on plants watered at the 0.1 g H~2~O/g soil level than at the 0.8 g H~2~O/g soil level, accounting for the interaction between aphid species composition and watering treatment. The mean population size of *R. padi* was significantly affected by watering treatment (*F* = 58.00; df = 1, 37; *P *= 0.001), but not by aphid species composition (*F* = 1.90; df = 1, 37; *P *= 0.18) or the aphid species composition × watering treatment interaction (*F* = 1.11; df = 1, 37; *P *= 0.30). When feeding on plants watered at the 0.1 g H~2~O/g soil level, mean *R. padi* population size was reduced by 70% compared to aphids feeding on plants watered at the 0.8 g H~2~O/g soil level ([Table 6](#nvx071-T6){ref-type="table"}). Table 6.Mean population sizes (± SE) of *Rhopalosiphum padi* and *Metopolophium festucae cerealium* after 21 d of unrestricted feeding on whole wheat plants at two levels of water stressAphid speciesWater treatmentAphid species treatment*R. padi* alone*M. festucae cerealium* aloneBoth species*R. padi*0.1 g H~2~O/g soil148 ± 146**--**161 ± 1390.8 g H~2~O/g soil493 ± 125**--**579 ± 125*M. festucae cerealium*0.1 g H~2~O/g soil**--**171 ± 2449 ± 220.8 g H~2~O/g soil**--**153 ± 20110 ± 20

Discussion
==========

Previous studies have demonstrated neutral ([@nvx071-B46], [@nvx071-B3]), negative ([@nvx071-B37], [@nvx071-B53]), or both ([@nvx071-B25]) effects of host plant water stress on aphid performance. However, interactions between aphid species have seldom been considered in the context of abiotic stress imposed on host plants ([@nvx071-B4]). Here, an amensal interaction was observed between *M. festucae cerealium* and *R. padi* in whole-plant assays, but the relative magnitude of the negative effect on *M. festucae cerealium* increased on water-stressed plants, while it remained consistent across water-stress treatments for *R. padi*. The asymmetrical interactions between these two aphid species corroborate findings from studies involving other aphid species. For example, the cereal aphids *Sipha flava* and *Rhopalosiphum maidis* exhibit asymmetrical competitive interactions on *Sorghum halepense* (L) Pers., and the outcome for each species depends on season (i.e., spring vs fall) in addition to the presence of natural enemies ([@nvx071-B22]). Contrasting results by [@nvx071-B21] found symmetrical competition between *R. padi* and *S. avenae* on seedling and tillering wheat plants, where reproductive rates of each species was negatively impacted by presence of the other. However, no abiotic stress was imposed on plants in these experiments.

Interactions between insect herbivores are commonly mediated by a shared host plant ([@nvx071-B20], [@nvx071-B41], [@nvx071-B42], [@nvx071-B19], [@nvx071-B14], [@nvx071-B31], [@nvx071-B22]). In many instances, insect herbivory can alter host plant quality to the benefit or detriment of the inducing insect and later-colonizing species ([@nvx071-B27], [@nvx071-B35], [@nvx071-B36], [@nvx071-B15], [@nvx071-B22]). Considering the generally poor nutritional quality of phloem sap, it has been suggested that some aphids manipulate phloem nutritional quality to their benefit, presumably through ultrastructural changes to plant cells induced by feeding on susceptible hosts ([@nvx071-B1]). [@nvx071-B50] demonstrated that the aphids *S. graminum* and *D. noxia* ingested phloem sap with a substantially higher concentration of amino acids than *R. padi* when feeding on both wheat and barley. Furthermore, *S. graminum* and *D. noxia* induced macroscopic chlorotic lesions to infested leaf tissues, as does *M. festucae cerealium*, whereas *R. padi* did not. It should be noted that these lesions are symptoms of feeding activity and do not directly indicate nutritional manipulation by the herbivore; however, other reports exist linking aphid-induced chlorotic lesions with the enhanced performance of aphids. For example, [@nvx071-B16] found that an *S. graminum* clone incapable of inducing chlorotic lesions on a particular wheat cultivar experienced enhanced performance on plants previously infested by another *S. graminum* clone which had the ability to induce lesions. We likewise observed distinctive chlorotic lesions on leaf tissues exposed to *M. festucae cerealium* feeding, compared with *R. padi* feeding which produced no visual changes to plant tissues. This inability by *R. padi* to induce such lesions has been reported in other studies (e.g., [@nvx071-B50], [@nvx071-B43]). In addition, whereas *S. graminum* foundresses induce changes that enhance growth of their nearby offspring, *R. padi* do not ([@nvx071-B47]). This indicates that *M. festucae cerealium* might be able to alter host plant nutritional quality, potentially to its benefit, as well as to the benefit of heterospecifics including *R. padi* when occupying the same leaf tissues. The interaction between water regime and presence of *R. padi* on *M. festucae cerealium* performance appears to stem from greater competition on water stressed plants, while no such interaction occurred for *R. padi* possibly because competitive effects are offset for *R. padi* by the documented increases in plant quality induced by cocolonizing *M. festucae cerealium*. While analyses of phloem sap are needed to directly support a hypothesis of feeding-induced nutritional enhancement ([@nvx071-B50]), the significant changes to *R. padi* life history traits on leaf tissue previously infested by *M. festucae cerealium* lead us to conclude that the mechanism(s) were plant mediated.

Basic biological information is limited for *M. festucae cerealium* in the PNW and in general ([@nvx071-B10]). However, surveys for *M. festucae cerealium* show seasonal overlap with *R. padi* elsewhere; outbreaks of both have been reported during April in Europe ([@nvx071-B40], [@nvx071-B32]) and on grasses in the spring ([@nvx071-B26]), and both aphids were detected in sweep net samples throughout the PNW during May and June of 2011 and 2012 ([@nvx071-B24], [@nvx071-B10]). Furthermore, [@nvx071-B13] observed evenly distributed *M. festucae cerealium* apterae within wheat plants in both laboratory and field settings. While spatial data were not recorded during our study, we observed generally even within-plant distributions for both aphid species when allowed to simultaneously colonize entire plants, showing no indications of avoidance or partitioning. Collectively, these findings suggest first, that *M. festucae cerealium* and *R. padi* both have the potential to infest wheat fields relatively early in the growing season and experience a temporal overlap; and second, that the distributions of both species cocolonizing the same wheat plant are not spatially separated, leading to potential colonization of the same (or closely neighboring) feeding sites. In surveys of more than 42 sites across the inland PNW taken during the booting stage of the crop in 2011--2014, *M. festucae cerealium* and *R. padi* were often found coinfesting individual plants and fields (S.D.E., unpublished data).

The likelihood of spatiotemporal overlap and potential for competitive interactions between *M. festucae cerealium* and *R. padi* in the PNW must be considered together with our findings of differential impacts of host plant water stress on each aphid species. While a significant negative effect occurred for *R. padi*, the same −0.7 MPa water stress in potted wheat plants had no effect on *M. festucae cerealium* unless *R. padi* was present. The capacity of *M. festucae cerealium* to colonize water-stressed wheat compared to *R. padi* may be partially due to differing life history traits. According to [@nvx071-B13], the highest reproductive rate achieved by *M. festucae cerealium* on cereals was 28 nymphs after 10 d, whereas *R. padi* can produce as many as 40 nymphs after 6--8 d ([@nvx071-B39], [@nvx071-B34]). In all present experiments, *M. festucae cerealium* reproduction consistently occurred 3 d following that of *R. padi* (i.e., prereproductive periods of 10 and 7 d, respectively). In the whole-plant assay average per-plant abundance of *M. festucae cerealium* on plants only infested with this aphid was ∼80% lower than *R. padi* when plants were adequately watered, and 70% lower than *R. padi* on water stressed plants ([Table 6](#nvx071-T6){ref-type="table"}). This differential was exacerbated by greater reproduction of *R. padi* on plants coinfested with *M. festucae cerealium*, enhancing *R. padi'*s competitive advantage on water-stressed plants. Further testing is needed to determine the underlying mechanisms responsible for this outcome.

Findings from this study have potential implications for pest management in cereal cropping systems in the PNW and elsewhere. Interspecific interactions between two pest aphid species were complex, producing unique outcomes for each species that depended on the environmental context (i.e., water stress). Thus, variation in drought incidence or other environmental stressors in the field might influence the prevalence and pest status of both species and result in emergent effects on the overall insect herbivore community. Continued monitoring for *M. festucae cerealium* and *R. padi* in the PNW has potential to reveal future ecological trends for each species at the population level. If both species continue to have sympatric distributions and cocolonize fields, competitive pressure by *R. padi* could displace *M. festucae cerealium* populations in drought years. Alternately, *M. festucae cerealium* infesting wheat fields where *R. padi* and other potential competitors are generally absent may remain relatively unaffected by adverse environmental conditions. An additional possibility is that cocolonization will lead to increased reproduction by *R. padi*, thus promoting greater plant injury or potential virus spread across a shorter temporal window. Our study did not address implications of these effects on yield of wheat infested by one, the other or both aphid species. Future work should use field studies to assess this, and to include assessments of other biotic components (e.g., natural enemies) and other climatic stresses associated with drought (e.g., elevated temperature). Finally, infection by BYDV also enhances *R. padi* performance on drought stressed plants ([@nvx071-B33], [@nvx071-B11]), which suggests the interaction of the combined stresses *M. festucae cerealium*, BYDV infection, and drought on *R. padi* should be assessed. Further investigations to these ends could help advance our understanding of the complex interactions between insect communities and the environment under projected conditions of increased drought frequency ([@nvx071-B9]).

We thank Ying Wu, Lana Unger, Bradley Stokes, Jenna Bjur, Carolyn McCotter, and Kennedy Caisley for their assistance in maintaining aphid colonies, conducting these experiments and for conducting pilot experiments. The work was funded under USDA NIFA Award 2011-68002-30191.

[^1]: Subject Editor: Joe Louis

[^2]: Values with letters denote Tukey's HSD test on the aphid treatment × water treatment interaction (among the four values for each species).

[^3]: Values followed by letters indicate Tukey's HSD test.

[^4]: Basic reproductive rate (*R~o~*) refers to average number of offspring produced per foundress.

[^5]: Generation time (*T*) represents average time (in days) between two consecutive generations.

[^6]: Intrinsic growth rate (*r~m~*) is the theoretical maximum population growth rate in the absence of density-dependent effects, where (dN/dt)(1/N) = *r*.