Write the aim: Strawberry (Fragaria × ananassa) is a fruit crop with high nutritional and economic value that is widely consumed and cultivated around the world. According to the Food and Agriculture Organization (FAO), the global production of strawberry was 9.22 million tons in 2018, with China, the United States, Mexico, Egypt, and Turkey being the top five producers (Rhouma et al., 2022). However, strawberry production and quality are severely affected by various microbial pathogens, especially those caused by E-coli 0157:h7 and Listeria monocytogenes, which can cause significant losses in yield and postharvest life. E-coli 0157:h7 and Listeria monocytogenes are common foodborne bacteria contaminating post harvested strawberries and can cause many diseases to humans such as hemorrhagic colitis and hemolytic uremic syndrome and many others. The current methods of controlling post harvested strawberry pathogens are mainly based on cultural practices and bactericidal applications, which are not very effective, and have negative impacts on the environment and human health. Moreover, there is a lack of resistant varieties of strawberry to different microorganisms, and the breeding programs are slow and costly. Therefore, there is an urgent need for developing a novel and sustainable approach for controlling foodborne bacteria and enhancing the quality of strawberries. In this project, we aim to develop and evaluate a new nano-coating containing bacteriocin as a sustainable and specific solution for postharvest protection of strawberries against foodborne bacteria it incorporated into nanocoatings for its ability to inhibit bacterial growth. In nanocoating applications, bacteriocin can be embedded within nanomaterials to enhance the antimicrobial properties of the coating. Nano-coating is a thin layer of nanomaterials applied to the surface of a substrate, which can modify its properties and functions. Nano-coating has been proposed as a promising alternative to conventional coatings for food preservation, as it can improve the delivery and efficacy of bioactive compounds, reduce the environmental impact and toxicity of agrochemicals, and overcome the problems of resistance and resurgence of pathogens. However, the development and application of nano-coating for food preservation face several challenges, such as the formulation and optimization of components and production techniques, the mechanism and mode of action of nano-coating on food pathogens, and the integration of wet lab experiments and computational analysis. This project aims to develop and evaluate a new nano-coating containing bacteriocin, employing a comprehensive approach that combines the utilization of genomic data, network analysis, and molecular docking, sonication, spray drying, and various analytical and biological techniques. By integrating these methods, we seek to reveal the molecular interactions and pathways involved in the antibacterial and quality-enhancing effects of the nano-coating, shedding light on its potential efficacy as a sustainable and specific solution for food preservation. The project will also evaluate the performance of the nano-coating in terms of stability, bioactivity, and safety, using various physicochemical, microbiological, and sensory tests. Furthermore, it will assess the cytotoxicity and biosafety of the nano-coating, using cell culture. The project will contribute to the advancement of science and technology in the field of nano-coating and food preservation, as well as to the improvement of strawberry production and quality.