i have presentation for the following topic give me possible questions with answers that my doctor will ask :   
 
• Study design :
This is a cross_sectional analytical study that carried out in February to May 2023 , by group(5) 3ed year medical laboratory student of Hadhramout in educational year 2022–2023, aimed to determine the iron deficiency anemia IDA among patient with Helicobacter pylori infection centers in Al-Mukalla city,Yemen.
• Study Population:
Individuals attending private hospitals in Al-Mukalla city who have been diagnosed with H. Pylori infection.
• Study area :
This study was conducted in three private hospitals In the city of Mukalla (Alburj consultant hospital – Hadhramaut modern hospital – Alarab hospital )Hadhramaut Governorate- in Yemen.
• Data collection methods & tools:
1. Face to face interviews
2. Laboratory investigation
 
• Face to face interviews:
A checklist was used to interview the patients.The checklist was designed by group (5)3rd year medical laboratory students. It was involved 3 main questions:
1. Age of the patient.
2. Time of onset of the infection.
3. The presence of the infection until the time of the study.
• Lab investigation:
The participants were asked to provide a stool sample according to the clean- cath procedure.
Sample were collected using a sterile container and transported to the laboratory within 1hrs of collection to confirm the H.pylori infection, and 5ml of blood were collected from participants for CBC  and serum ferritin tests to detect the presence of anemia in the patients .
This tests were obtained by using:
1. H.pylori antigen rapid test (stool):
H. pylori Antigen Rapid Test is an in vitro qualitative immunochromatographic assay for the rapid detection of Helicobacter pylori antigens in human stool specimen. The test results are intended to aid in the diagnosis of H. pylori infection, to monitor the effectiveness of therapeutic treatment and to confirm the eradication of H. pylori in pqualitative
Patients. 
2. Cobas E411 analyzer for immunoassay tests:
The cobas E411 analyzer is a fully automated analyzer that uses a patented ElectroChemiLuminescence (ECL) technology for immunoassay analysis. It is designed for both quantitative and qualitative in vitro assay determinations for a broad range of applications (including anemia; bone, cardiac and tumor markers; critical care; fertility/hormones; maternal care; and infectious diseases).
Ferritin is the iron storage protein. The ferritin detectable in human serum is in equilibrium with the body's depot iron and hence acts as an indicator for the level of iron stores. Determinations of ferritin can be used as an aid in iron metabolism diagnosis, monitoring iron therapy, ascertaining the iron reserves in group at risk and in the differential diagnosis of anemias.
Normal range of serum ferritin:
Male:24_336 ng/ml
Female:24_307 ng /ml
3. Hematology analyzer:
Hematology analyzers are used widely in patient and research settings to count and characterize blood cells for disease detection and monitoring. Basic analyzers return a complete blood count (CBC) with a three-part differential white blood cell (WBC) count. Sophisticated analyzers measure cell morphology and can detect small cell populations to diagnose rare blood conditions.
A complete blood count (CBC) test to study the patient’s red blood cell (RBC) profile and ascertain the presence of anemia, iron deficiency, or both.
Normal range of Hb:
Male:13.8 _17.2 g/dl
Female:12.1_15.1 g/
4. Spectrophotometer:
A spectrophotometer is a laboratory equipment that can measure the number of photons (the intensity of light) absorbed after passing through the solution of the sample. It can also detect the concentration of the solution by measuring the intensity of detected light. “Spectrophotometry” is the method used by the spectrophotometer for measuring the amount of light absorbed by any chemical substance when a light beam passes through it.
An ultraviolet spectrophotometric method for the determination of iron utilizes the absorption of the ferric ion in perchloric acid solution. The system shows excellent stability, good sensitivity, and adherence to the usual absorption law.
Normal range of serum iron:
Male:80_180 mcg/dl
Female:60_160 mcg/dl
Data collection tools:
• Cell pak diluent
• 4DI bottle 2L
• sulfolyzer 500ml Bottle
• 4Ds bottle
• material Alcohol swaps
• Gloves
• Cleaner bottles
• Micropipette (100-1000)
• Syringes
• Tourniquets
• Racks
• Wound plasters
• EDTA tubes
• Gel tubes
• Yellow tips
• Blue tips
• cuvette Boxes
• Assay tips
• Assay cups
• Clean cells
• Pro cells
• Stool containers
• The Sampling:
Sample size:
Based on previous research, we assume that around 50% of individuals in the study population are infected with H. pylori and that the prevalence of IDA in the infected population is 40%.
Using a 95% level of confidence, a margin of error of 5%, and the estimated prevalence, we can calculate the required sample size as follows:
n = (Z^2 * p * q) / E^2
Where:
n = required sample size
Z= the standard normal deviate, set at 1.96 for a 95% confidence interval
p = estimated prevalence (0.4)
q = (1-p) = 0.6
E = margin of error (0.05)
n = (1.96^2 * 0.4 * 0.6) / 0.05^2
n = 368.16
Based on this calculation, a minimum sample size of 368 individuals will be required to estimate the prevalence of IDA among H. pylori patients in Al-Mukalla city.
However, to account for potential dropouts or incomplete data, we will increase the sample size by 10%, giving a final estimated sample size of 405 individuals.
Sample method:
The sampling method stratified random sampling.
Study Variables:
1. Independent variable:
-H. Pylori infection
2. Dependent variable:
- Iron deficiency anemia (IDA)
3. Confounding variables:
- Age
- Gender
- Medical history