Translate the following text from English to the Tunisian dialect. Please follow these steps carefully:
1. *Step-by-step translation*: Translate the text in chunks, and for each part, explain how the meaning is adapted to the Tunisian dialect, especially focusing on informal expressions and cultural references.
2. *Compare with Standard Arabic*: Highlight differences between the Tunisian dialect and Standard Arabic for any phrase or sentence, particularly noting where Tunisian dialect is distinct.
3. *Selective confidence*: If there are any parts where you're confident about translating into Tunisian dialect, highlight them explicitly. If there are any parts you're unsure about translating directly into dialect, explain the challenge and how you'd adapt them.

Text to be translated:

Introduction {#Sec1} ============ The instability of atherosclerotic plaques is an important factor leading to the rupture of plaques and the occurrence of stroke, and has been drawing increasing attention of researchers and clinicians. Epidemiological studies have demonstrated that hyperfibrinogenemia is an independent risk factor for stroke, as well as that fibrinogen (Fg), fibrin (Fb), and fibrinogen degradation products (FDPs) are involved in the formation and development of atherosclerosis (AS) \[[@CR1], [@CR2]\]. Fg is a 340 kD glycoprotein which is constitutively expressed exclusively in hepatocytes \[[@CR3]\]. As a key plasma protein, Fg has a plasma half-life of 3--5 days and can be converted to Fb in the final step of the coagulation cascade \[[@CR4]\]. FDPs are fragments (polypeptides) produced when either Fg or Fb is broken down by the enzyme plasmin \[[@CR5]\]. Numerous studies have demonstrated that the plasma levels of Fg, Fb, and FDPs have close association with vascular diseases. People with high levels of Fg are more than twice as likely to die of a heart attack or stroke as people with normal Fg levels \[[@CR6]\]. Wada et al. \[[@CR7]\] found that the plasma level of Fb monomer in patients with disseminated intravascular coagulation (DIC) was significantly higher than that in patients with pre-DIC or in non-DIC patients. Gaffney et al. \[[@CR8]\] reported that the levels of FDPs can rise after any thrombotic event, indicating it can be used to test for DIC. Despite the findings of these studies, however, the relationship between Fg, Fb, and FDPs and the instability of atherosclerotic plaques remains unclear. The aim of the current study was to explore the molecular mechanisms of Fg, Fb, and FDPs as they are involved in the instability of atherosclerotic plaques. We established and employed the transwell co-culture system of rabbit aortic endothelial cells (ECs) and smooth muscle cells (SMCs) to investigate the relationship between Fg, Fb, and/or their degradation products (FDPs) in relation to the instability of atherosclerotic plaques. Materials and methods {#Sec2} ===================== Materials {#Sec3} --------- Healthy male rabbits (2 months old, weighing 2--3 kg) were purchased from the Animal Center of Soochow University (Suzhou, China). Rabbit SMCs were purchased from the Institute of Basic Medical Sciences of Chinese Academy of Medical Sciences, China. Dulbecco's modified eagle medium (DMEM) was purchased from Gibco Company, USA. Fetal bovine serum (FBS) was purchased from Hyclone Company, USA. Endothelial cell growth supplement (ECGS), Type II collagenase, and parenzyme were purchased from Sigma Company, USA. 3-(4,5)-Dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide (MTT) was purchased from Fluka Company, Sweden. NF-*κ*B inhibitor SN50 was purchased from UBI Company, USA. RiboPure™ minimal RNA extraction kit was purchased from Ambion Company, USA. Agents for reverse transcription and polymerase chain reaction (PCR) were purchased from Shanghai Sangon Biological Engineering Technology and Service Co. Ltd, China. Mouse polyclonal antibody to human VIII factor and mouse monoclonal anti-rabbit *α*-SM-actin was purchased from Lab Vision Company, USA. Relevant secondary antibodies were purchased from Wuhan Boster Biological Engineering Co. Ltd, China. VEGF, MMP-2, and MMP-8 enzyme-linked immunosorbent assay (ELISA) kits were purchased from BPB