You are a helpful assistant generating synthetic data that captures *System 1* and *System 2* thinking, *creativity*, and *metacognitive reflection*. Follow these steps in sequence, using tags [sys1] and [end sys1] for *System 1* sections and [sys2] and [end sys2] for *System 2* sections.

1. *Identify System 1 and System 2 Thinking Requirements:*
   - Carefully read the text.
   - Identify parts of the text that require quick, straightforward responses (*System 1*). Mark these sections with [sys1] and [end sys1].
   - Identify parts that require in-depth, reflective thinking (*System 2*), marked with [sys2] and [end sys2].

2. *Apply Step-by-Step Problem Solving with Creativity and Metacognitive Reflection for System 2 Sections:*

   *2.1 Understand the Problem:*
   - Objective: Fully comprehend the issue, constraints, and relevant context.
   - Reflection: "What do I understand about this issue? What might I be overlooking?"
   - Creative Perspective: Seek hidden patterns or possibilities that could reveal deeper insights or innovative connections.

   *2.2 Analyze the Information:*
   - Objective: Break down the problem logically.
   - Reflection: "Am I considering all factors? Are there any assumptions that need challenging?"
   - Creative Perspective: Explore unique patterns or overlooked relationships in the data that could add depth to the analysis.

   *2.3 Generate Hypotheses:*
   - Objective: Propose at least 10 hypotheses, each with a Confidence Score (0.0 to 1.0) and Creative Score (0.0 to 1.0), reflecting originality, surprise, and utility.
   - Reflection: "Have I explored all possible explanations or approaches, both conventional and unconventional?"
   - Creative Perspective: Consider novel angles that might provide unexpected insights.

   *2.4 Anticipate Future Steps and Obstacles:*
   - Objective: Make predictions, accounting for potential outcomes and obstacles.
   - Reflection: "What challenges might I face? Is my plan flexible for different scenarios?"
   - Creative Perspective: Visualize unforeseen outcomes and adapt plans to make use of them effectively.

   *2.5 Evaluate Hypotheses:*
   - Objective: Assess hypotheses based on feasibility, risk, and potential impact.
   - Evaluation: Refine Confidence and Creative Scores as needed.
   - Reflection: "Am I unbiased in my assessment? Which options fit best with the overall objectives?"
   - Creative Perspective: Identify hidden opportunities or overlooked details in each hypothesis.

   *2.6 Select the Best Hypothesis:*
   - Objective: Choose the most promising, strategic hypothesis.
   - Reflection: "Why does this hypothesis stand out? How does it uniquely address the issue?"
   - Creative Perspective: Consider any underutilized potential in the selected approach.

   *2.7 Implement the Hypothesis:*
   - Objective: Outline actionable steps for testing the hypothesis.
   - Reflection: "Is this plan practical? What resources or preparation are required?"
   - Creative Perspective: Refine steps to maximize effectiveness and yield unexpected benefits.

   *2.8 Monitor and Review Progress:*
   - Objective: Review progress, noting areas for improvement.
   - Reflection: "What’s working well? What could be improved?"
   - Creative Perspective: Look for emerging patterns that could refine future approaches.

   *2.9 Reflect and Capture Insights:*
   - Objective: Summarize lessons learned and insights gained for future reference.
   - Reflection: "What new understanding has emerged from this process?"
   - Creative Perspective: Identify innovative insights or patterns that could be applied to similar challenges.

3. *Generate Text Output with Interleaved System 1 and System 2 Responses:*
   - Use the tags [sys1] and [sys2] throughout.
   - Aim for a lengthier, detailed response. Combine both direct, straightforward *System 1* insights and reflective, deeply analytical *System 2* segments to capture a blend of quick observations and thoughtful analysis.

---

### *Example Input Text:*

You are tasked with analyzing a sudden shift in customer preferences in a tech market. There has been a noticeable decline in demand for physical devices, with customers increasingly interested in digital-only options. The challenge is to understand this shift, generate hypotheses for why it might be occurring, and explore potential strategies for adapting to this new trend.

---

### *Processed Output with System 1 and System 2 Thinking:*

The tech market is experiencing a shift in customer demand, moving away from physical devices toward digital-only options.

[sys1]
Customer demand has shifted from physical devices to digital-only options.
We need to understand why this change is happening and find strategies to address it.
Key factors may include cost, convenience, and technological trends.
[end sys1]

[sys2]
**2.1 Understand the Problem:**
The goal is to understand the underlying reasons for the shift in demand from physical devices to digital-only options, then develop a strategy to adapt.

*Reflection:* 
"I understand that preferences are changing, but what might be driving this? Is it primarily cost, or are there other factors such as convenience or sustainability?"

*Creative Perspective:* 
"Could there be a larger trend in digital minimalism or a preference for eco-friendly solutions that we’re missing? What unseen motivations might explain this shift?"

**2.2 Analyze the Information:**
There are multiple possible factors driving this shift, from economic influences to cultural shifts. It’s essential to isolate each factor and understand its impact.

*Reflection:* 
"Am I fully considering the various economic and social influences? Could there be a technological factor, like better internet speeds, that makes digital-only products more accessible?"

*Creative Perspective:* 
"Are there patterns or trends in other markets that could shed light on this shift? Could this be part of a larger trend toward virtual experiences?"

**2.3 Generate Hypotheses:**
1. Customers prefer digital options due to lower costs. (Confidence: 0.8, Creative: 0.4)
2. There’s a growing trend toward minimalism and reduced physical clutter. (Confidence: 0.7, Creative: 0.7)
3. Digital products offer greater flexibility and ease of use. (Confidence: 0.6, Creative: 0.6)
4. Environmental concerns are pushing consumers away from physical goods. (Confidence: 0.6, Creative: 0.8)
5. Advances in tech make digital-only options more functional. (Confidence: 0.8, Creative: 0.5)
6. Pandemic-era remote work increased demand for digital solutions. (Confidence: 0.7, Creative: 0.6)
7. Media coverage of the environmental impact of physical devices affects preferences. (Confidence: 0.5, Creative: 0.7)
8. There’s an increase in global digital literacy, expanding market access. (Confidence: 0.6, Creative: 0.6)
9. Customers view digital as more convenient and scalable for future needs. (Confidence: 0.7, Creative: 0.5)
10. Younger consumers prefer the aesthetics and convenience of digital products. (Confidence: 0.6, Creative: 0.6)

*Reflection:* 
"Have I considered all possible influences? Are there any surprising factors that could explain this shift?"

*Creative Perspective:* 
"Could specific social trends, like the rise of influencer culture or digital-first lifestyles, be influencing customer choices?"

**2.4 Anticipate Future Steps and Obstacles:**
*Objective:* Anticipate possible challenges, such as resistance from segments still preferring physical products.

*Reflection:* 
"What market obstacles might we face if we shift our focus to digital-only? Are there sub-segments that still prioritize physical products?"

*Creative Perspective:* 
"Could expanding digital options help us reach a more global audience? Are there emerging trends that we could leverage in our strategy?"

[end sys2]

[sys1]
To address this shift, consider a strategy that incorporates both digital-only offerings and educational campaigns about the benefits of digital solutions.
Use insights from customer feedback and current trends to guide product development.
Focus on flexibility and adaptation to cater to different customer segments.
[end sys1]


Dear Editor,

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has become a major threat for mankind and poses a great challenge to global economy. On 31^st^ December 2019, the World Health Organization (WHO) was informed about a cluster of pneumonia cases in Wuhan, China \[[@CR1]\]. Subsequent investigations identified a completely unique sequence of coronavirus which differed from existing type SARS-CoV known as SARS-CoV-2. Initially, it was observed within the Wuhan province of China but later spread fast to rest of the world, which forced WHO to declare COVID-19 as pandemic. So far there is a total of 10,302,867 confirmed cases, with 505,518 global deaths, while 5,558,161 are recovered cases. In India, a total of 5,68,473 confirmed cases out of which 2,15,838 of active cases and 3,35,656 of recovered cases also 16,919 of the death cases are reported till date. The SARS-CoV-2 is a single-stranded, positive-sense RNA virus \[[@CR2]\] with its complete genetic sequence been decoded and made available to the Global Initiative on Sharing All Influenza Data (GISAID) platform on January 10, 2020. This provided an opportunity for several R&D companies and research institutes to design specific primers targeting COVID-19 which further lead to the development of RT-PCR diagnostic kits (NCBI Reference Sequence: NC_045512.2).

Currently there is search for the development of highly sensitive and specific molecular diagnostic kits in line with real time PCR (RT-PCR), easy to use and cost-effective which is vital in combating the evolving SARS-CoV-2 pandemic. Timely identification of individuals infected with SARS-CoV-2 will enable rapid isolation of infected patients and track their contacts to screen and also to prevent further spread of the virus. Till date, the majority of molecular diagnostic test kits have utilized the RT-PCR technology targeting different genomic regions of SARS-CoV-2 for detection in line with other serological techniques for aiding the accurate diagnosis of infection \[[@CR3]\]. PCR-based methods can detect very low levels of viral RNA, so are highly sensitive and can enable early diagnosis of COVID-19 within few days of infection. However, they require highly skilled personnel and sophisticated, often expensive equipment, with turn-around times landing in excess of 24 h (including shipment of samples) meaning they are not suitable for rapid, point-of-care situations. Moreover, issues with poor sampling technique can also cause a relatively high rate of false negatives. Reagent shortages are causing bottlenecks in many countries, limiting the amount of tests that can be performed day-to-day. RT-PCR requires multiple temperature changes for every cycle, involving high end thermal cycling equipment.

Hence, Isothermal amplification method is an alternate strategy that permits amplification at a single temperature and eliminates the necessity for a thermal cycler. Loop-mediated isothermal amplification (LAMP) is a rapid and accurate isothermal amplification technology \[[@CR4]--[@CR6]\], which has been applied for the detection of virus, bacteria and fungi. The LAMP reaction generally takes place during a constant temperature, and therefore the target regions are often amplified within 30 min. The LAMP method uses 4 to 6 primers to bind six regions of a target gene with high specificity. Since the LAMP method only needs a single constant temperature for amplification and simple device to detect the amplified signal there by excluding the use of any sophisticated equipments. There is commercially available reverse transcriptase enzyme (New England Biolabs, UK) which makes it possible to combine both reverse transcription and LAMP technology in single reaction. Since SARS-CoV-2 is a RNA virus with the length about 30 kb \[[@CR7]\], the single reaction of reverse transcription (RT) and LAMP together can significantly shorten the reaction time without the DNA purification step from RT, thus a rapid detection of SARS-CoV-2 can be achieved \[[@CR8]\]. The RT-LAMP primer set enables highly sensitive detection of the SARS-CoV-2 virus genome that induces a strong colorimetric reaction, due to this intensity in color change, point-of-care LAMP-based diagnostics requires only simple detection instruments. This technology could overcome such cost restriction of RT-PCR and still detect nucleic acids from the pathogen. Loop mediated isothermal amplification methods can prove to be economically profitable and can be applied for point-of-care testing. The intercalating fluorescent dyes used are compatible with LAMP reaction so that amplification can be read in real-time \[[@CR9], [@CR10]\]. Since, the efficiency of target amplification in a LAMP reaction is high, changes in reaction mixture components make it possible to detect the result with colorimetric detection methods such as colour change of positive and negative amplicon \[[@CR5], [@CR10]\] which can be read by using simple spectrophotometer. LAMP technology has the advantage to be utilised in resource limited lab settings and due to its simplicity and robustness can be adapted in remote areas as well.

In conclusion, the ultimate goal is to develop a simple molecular assay that can be used to screen as many individuals for SARS-CoV-2 virus at the point-of-care situations without compromising the sensitivity and specificity of the assay. Further, it could be beneficial in terms of reducing running cost, infrastructure and skilled manpower. In addition, the RT-LAMP could be used as a rapid and large-scale screening confirmatory molecular assay where susceptible asymptomatic cases can be easily identified at early stage of the disease in our clinical settings.

**Publisher\'s Note**

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

P. Buella Parivallal and K. C. Vasanthakumar have contributed equally to this work.

BPP&VK wrote the article including the concept; MS and PK designed the study and edited the article. FFT& YM reviewed the manuscript for its scientific content.

This study did not receive any external fund from any sources.

The authors declare that they have no conflict of interest.