of the following study tell my why that i will chose stratified random sampling and how i will use it for the following information : Iron deficiency anemia (IDA) is the predominant type of anemia in developing countries especially in Yemen [1,2].  Results from  iron imbalance  (shortage of iron in patient's body). Usually, deficiency of iron goes unnoticed. develops gradually and does not have clinically apparent symptoms until the body become more deficient in iron and anemia worsens  [3]. Ferritin is the Best indicator of IDA, and low ferritin alone is diagnostic of iron deficiency anemia[4] unexplained iron defciency anaemia in patients with H. pylori-positive gastritis can demonstrated that
H. pylori cure led to a prolonged remission of iron dependence and to the resolution of iron defciency anaemia. [5] .H. pylori is gram negative micro earophilic spiral shaped flagellated bacillus which colonize the mucus layer of gastric epithelium which infects approximately one-half of the world population, making it one of the most common bacterial infection. [1 1. It effects in seurm hemoglobin and serum ferritin level and increase iron losses,Helicobacter pylori (H. pylori) has been considered as a major cause for the development of peptic ulcer disease and gastric malignancy. Recent studies have shown that H. pylori can also cause other extra gastric diseases. [2] . H. pylori appears to be linked to hematological disorder such as idiopathic thrombocytopenic purpura, vitamin B12 deficiency and unexplained iron deficiency anemia.[1] Recently several sero epidemiologic studies have suggested a link between iron deficiency anemia and H. pylori infection. [ ] according to certain research, H.Pylori colonization of the stomach mucosa may hinder iron intake, increase iron loss, and possibly cause IDA.[ ] H. pylori need iron to survive, it is one of the few microbes that can obtain it both directly from hemoglobin's heme and indirectly from lactoferrin, which is present in human. [ ] meta-analyses showed that H. pylori eradication combined with iron administration was more effective than iron administration alone for the treatment of IDA, which recommends testing and treatment for H. pylori infection in patients with unexplained IDA, This indicates a clear link between iron deficiency anemia and H.pylori infection. [ ] H. pylori affects individuals of different age groups, the elderly have a higher prevalence of H. pylori infection, (aged >60 years, it was 39.4%) and risk of developing atrophic gastritis, and stomach cancer As well, People often get H. pylori infection during childhood [1].
Risk factors for H. pylori infection are related to Living in crowded conditions, Living without a reliable supply of clean water, Living in a developing country, Living with someone who has an H. pylori infection [1].
Malabsorption a dietary assessment should be made in all patients to ascertain their iron intake. gastric acid is required to release iron from food and helps to keep iron in the soluble ferrous state .hypochlorhydria in this patient or that due to drugs such as proton pump Inhibitors may contribute to the lack of iron availabil ity from the diet as may previous gastric surgery [2]. 
Studies in the literature have reported that H. pylori infection could be a potential cause of iron-deficiency anemia (IDA) [2].The aim of the  study is to investigate the prevalence of iron deficiency anemia among H. Pylori patients in private hospitals in Al-Mukalla city and to measure the accossiated risk factor that may increase the probability of iron deficiency anemia cases. The study importance emerges from the small number of studies in our country that have been conducted on iron profile in patient with Helicobacter pylori infection especially in Hadhramout governorate. This current study will estimate the iron profile and  help to detect the IDA in patient with H. pylori infection.