Twelve years after competing in her last Olympics, Skarlatou qualified for her second Greek team, as a 36-year-old, in the RS:X class at the 2012 Summer Olympics by receiving a berth from the ISAF Sailing World Championships in Perth, Western Australia. Though she fell short of her slot for the medal rounds, Skarlatou achieved her best Olympic result with a sixteenth-place effort in a fleet of twenty-six sailors, accumulating a net score of 134 points.