Mariene began her professional career as a backing vocalist for Timbalada, Carlinhos Brown and Marcia Freire. She began her solo career in 1997. In 2001 she won a Caymmi Award for Best Artistic Production. Her first album was released in 2005. Mariene was made the queen of the 2007 Gay Pride parade in Salvador by the Grupo Gay da Bahia. She performed at the 2016 Rio Olympics closing ceremony with the song "Pelo Tempo Que Durar" du Marisa Monte in front of the Olympic Flame before it was extinguished.