This regiment reported 18 casualties at Camp Alleghany, 72 at McDowell, and 29 at Cross Keys and Port Republic. It lost 1 killed and 24 wounded at Cedar Mountain, had 3 killed and 20 wounded at Sharpsburg, and reported 1 killed and 13 wounded at Fredericksburg. Of the 280 engaged at Gettysburg, twenty-five percent were disabled. There were 15 members (1 officer, 14 enlisted) of the 25th at Appomattox on April 9, 1865.