Petosky is politically active. In 2002, she edited and animated the political videos for Bushboy.com, cited as pioneering Internet political parody videos pre-YouTube. The popularity of these video led to Petosky doing paid work for Arianna Huffington, Al Franken, and working with Eric Utne to create videos promoting instant-runoff voting. In 2016, in response to "statements of love and images" of men kissing, and in defiance of the homophobic feelings of the gunman Omar Mateen, who perpetrated the Orlando shooting, she posted "a collage of male couples kissing", which she says caused her to immediately lose "200 followers on Twitter," although the post was "liked more than a thousand times." One year later, when interviewed by GLAAD, she stated that she has seen "negative consequences" for simultaneously working in the entertainment industry and being an early "vocal advocate for more fair and accurate representations of transgender people in the media," noting that some people have been scared to meet with her, with some telling her to tone down her posts, although others supported her. As such, she called for trans people to do work that is visible, and said it is weird that LGBT people are "underrepresented in the telling of their own cultural stories." She said something similar in a 2015 interview. Petosky also stated, in 2018, that Amazon told her to "tone down" her political messages, claiming that posting feminist messages was akin to supporting Donald Trump, and saying she should stop being as critical of the TSA.