During his Serie A club career, Bertini played for several Italian teams: Empoli (1963–64), Fiorentina (1964–68), and most notably Inter Milan (1968–77), where he remained for nine seasons, winning the Scudetto in 1971; he had previously also won a Coppa Italia and a Mitropa Cup double with Fiorentina in 1966. He also won the Serie C in 1963 whilst playing with Prato for a season (1962–63). He ended his career in Serie B, in 1978, after a season with Rimini (1977–78).