In the 2016 Olympic Games in Rio de Janeiro, Mukasakindi again competed in the women's marathon, one of seven Rwandans to compete in the games. She hoped to improve on her performance in London, and spent significant time preparing in Kenya and Italy, as well as in her homeland. Despite this training, her performance in the event was poor. Mukasakindi finished 126th in the race, out of a field of 133, and posted her worst competitive time at 3:05:57. She blamed the result on an ankle injury she had sustained in training, three days before the event; she had not thought it a problem before the race, but the ankle began causing her problems into the race, worsening after . She continued to finish the race, but cited the injury as the major reason for her poor performance.