During the 1948 war the Negev Brigade was involved in the defence of Negba, Kafr Daron, Be'erot Yitzhak and Nirim. In October 1948 the Brigade took part in the conquest of Beersheba. They were also part of Operations Barak, Yoav, An Far, Horev, Uvda and Pleshet. In the 1967 war the Brigade fought in the battle of Abu Agelia.