The regiment fought in the Appomattox Campaign between 28 March and 9 April, during which it participated in the Union breakthrough at Petersburg on 2 April. The regiment prepared for the assault along with the rest of the division on the night of 30 March, but it was postponed until the morning of 2 April. After marching to the camp of the 207th Pennsylvania just before midnight on 1 April, the 211th moved to the front at 03:30 on 2 April, and took positions alongside the brigade with its left on the Jerusalem Plank Road. Lieutenant Albert Alexander of Company D commanded a detail of pioneers from the brigade, which cut down the abatis and chevaux de frise in front of the Confederate positions despite artillery fire. Behind the pioneers, the division advanced, with the 211th closely behind the 207th and 205th Pennsylvania in the column of the brigade. Dodd led the 211th and elements of the 207th towards Fort Mahone after the initial breakthrough and captured it, then brought artillerymen up to turn the guns around. The regiment repulsed multiple counterattacks in fierce fighting, suffering a total of 135 casualties: four officers and seventeen men killed, four officers and 89 men wounded, and 21 missing. Among the dead were Lieutenant Colonel Charles McLain and Alexander, while Major Elias B. Lee was mortally wounded. Privates John C. Ewing of Company E and Amzi D. Harmon of Company K were awarded the Medal of Honor for capturing the flags of the 61st Alabama and the 45th North Carolina, respectively.