The Ki-32 saw extensive war service in the Second Sino-Japanese War, equipping the 3rd, 6th, 10th, 35th, 45th, 65th and 75th "Sentai". It also saw combat during the Battle of Nomonhan against the Soviet Union in 1938-1939. Its last combat action was bombing Commonwealth forces during the Japanese invasion of Hong Kong in December 1941. After their withdrawal from front-line service in 1942, the Ki-32s were used in a training role.