"S35", as part of the 9th Torpedo Boat Flotilla, took part in the Bombardment of Yarmouth and Lowestoft on 24–25 April 1916, where she formed part of the escort for the battlecruisers of the I Scouting Group. At the Battle of Jutland on 31 May – 1 June 1916, "S35" was part of the 18th Half-Flotilla, 9th Torpedo Boat Flotilla, again operating in support of the I Scouting Group. The 9th Flotilla, including "S35", took part in a torpedo attack on British battlecruisers from about 17:26 CET (16:26 GMT). The attack was disrupted by British destroyers, with the German torpedo boat hit by a torpedo from the British destroyer . "S35", along with rescued "V29"s crew before "V29" sank. was disabled by British shells in this clash and was scuttled by gunfire from . On the British side, the destroyer was disabled by a hit in the engine room and later sunk. Later during the day, at about 19:00 CET, the 9th Flotilla attempted another torpedo attack against British battlecruisers, which was curtailed by poor visibility and an attack by British destroyers. From about 20:15 CET (19:15 GMT), "S35" took part in a large-scale torpedo attack by the 6th and 9th Torpedo Boat Flotillas on the British fleet in order to cover the outnumbered German battleships' turn to the west. The German torpedo boats came under heavy fire from British battleships, and while "S35" fired two torpedoes at the British line, she was hit by two heavy shells, probably from a salvo fired by the battleship s main armament. "S35" broke in two and sank killing all 88 of her crew along with the survivors from "V29" who were aboard. None of the torpedoes fired by the Germans found their targets, although several narrowly missed British battleships, forcing them to take avoiding action.