At the start of 2001, Mazzacane tested for Arrows, but finally settled into the Prost team, taking over the seat of the Sauber-bound Nick Heidfeld. He beat CART's Oriol Servia for the Prost spot, and was announced as the second driver in January . However, the 2001 San Marino Grand Prix was his final Formula One race. Alain Prost fired him by using a performance clause in his contract, and the vacancy was filled by Luciano Burti, who had recently been sacked from Jaguar Racing.