After a short period of re-training in Australia to prepare for jungle warfare, the battalion was deployed to New Guinea in August 1942 as the Australians sent reinforcements to the Kokoda Track to fight against Japanese forces that had been advancing towards Port Moresby. After the Japanese were forced to exhaust their supplies they began to fall back towards their beachheads on the north coast. The 2/14th was part of the Australian advance that then saw further action around Gona. In September 1943, after a period of re-organisation in Australia, the battalion took part in the advance on Lae as the Allies went on the offensive in New Guinea, before taking part in the fighting in the Markham and Ramu Valleys of the Finisterre Range campaign. The battalion's final involvement in the war came in the landing on Balikpapan in 1945. The 2/14th was disbanded after the war, in early 1946.