The 44th subsequently served with Kirkland's Brigade and MacRae's Brigade, fighting in the Bristoe Campaign. During the Battle of Bristoe Station, part of the Bristoe Campaign, the regiment reported losses of 23 killed and 63 wounded. It suffered heavy casualties during the Battle of the Wilderness before fighting in the Battle of Spotsylvania Court House and the Battle of Cold Harbor during the Wilderness campaign. From late 1864 to the northern hemisphere spring of 1865, the regiment fought in the Siege of Petersburg south of the James River, in which it again suffered heavy casualties.