Barksdale was at first base on August 7, 2004, for Greg Maddux's 300th win and also at first base on June 1, 2012, when Johan Santana threw a no-hitter. He has also umpired in the World Baseball Classic in 2009 and 2013, the Major League Baseball All-Star Game in 2012, the Wild Card Game in 2013, 2017 and 2020, the Division Series in 2014, 2015, 2018 and 2019, the Championship Series in 2017 and 2020 and the World Series in 2019.