Chambers began 2014 by finishing second in the 60 m at the British Indoor Championships, before going on to finish sixth in the 60 m final at the World Indoor Championships in a time of 6.52s. This was the fastest time he had run the 60m in since 2010. In his first three 100 m races of the outdoor season he failed to break 10.20, before hitting form during the British Championships. Going into the event, he was only ranked 10th in the UK, but he won the 100 m final, defeating Chijindu Ujah, who had broken 10 seconds three weeks earlier. Chambers' victory guaranteed him selection for the European Championships as his winning time was 10.12 secs, inside the European qualifying mark of 10.20. This was his fifth consecutive win at the British Championships. Chambers won his final medal in international competition at the 2014 IAAF World Relays in May. At the 2014 European Championships in August, Chambers missed out on a final individual medal by 0.02 seconds, finishing 4th in the 100m final in 10.24. This would be his last appearance at a major championships.