Highlights of the service of this regiment include distinguished actions at the Battle of Fort Donelson and at the Battle of Shiloh, where about 80 members of the regiment were casualties, either dead or wounded. The regiment fought in the subsequent Battle of Corinth and later in the Atlanta Campaign, including at the Battle of Atlanta, continuing on to South Carolina and to Goldsboro, North Carolina, in the Carolinas Campaign. With the surrender of the Confederate States Army under Joseph E. Johnston, the 2nd Iowa traveled to Washington, D.C., for the Grand Review of the Armies of the Union on May 23–24, 1865.