In the late 19th and early 20th century, Berovo was part of the Kosovo Vilayet of the Ottoman Empire. As a result of the Balkan Wars, the town was included in the area ceded in 1913 to the Kingdom of Serbia, which in 1918 joined the Kingdom of Serbs, Croats and Slovenes (renamed Kingdom of Yugoslavia in 1929). From 1929 to 1941, Berovo was part of the Vardar Banovina of the Kingdom of Yugoslavia. From 1941 to 1944, during the Axis occupation of Yugoslavia, Berovo, as most of Vardar Macedonia, was annexed by the Kingdom of Bulgaria.