On November 25, 2019, Zunino signed a one-year deal to remain with the Rays in 2020. Zunino finished the 2020 season batting .147 with 4 home runs over 28 games. The Rays finished the season with the best record in the American League. Through the American League playoffs, Zunino hit four home runs with eight RBIs. The Rays won every game in which Zunino recorded an RBI, including game 7 of the American League Championship Series against the Houston Astros. The Rays advanced to the World Series against the Los Angeles Dodgers, where they lost in six games.