The plague of 1485 greatly influenced the painting by way of the inclusion of St. Sebastian and his opposite, St. Job. The arrow at St. Sebastian’s side symbolizes the plague, and his presence in the painting would be an invocation used in response to the plague that was hitting Venice at the time. St. Job symbolizes suffering through his plague-like illness, through which he relies on his faith to endure. Not only were both St. Sebastian and St. Job associated with suffering and pain, but the Scuola di San Giobbe was originally a hospice that was built in response to a plague, hence the representation of these saints and their faith to give hope to patients.