On the first day of the Olympics, Titenis recorded the third fastest qualifying time of 59.68 in the preliminary heats of the 100 m breaststroke by six hundredths of a second (0.06) behind Australia's Christian Sprenger. He lowered his time to 59.66 in the semifinals, but was unable to break a minute barrier, when he finished eighth in the final by nearly a second behind Italy's Fabio Scozzoli at 1:00.84. Achieving his best finish, Titenis became the second Lithuanian swimmer in history to reach an Olympic final since Vytautas Janušaitis did so in 2004, until his teammate Rūta Meilutytė surprisingly won the gold medal in the same stroke by the following evening.