On 4 September, under Italy's new manager Antonio Conte, De Rossi wore the captain's armband in a 2–0 friendly home win over the Netherlands in Bari, also scoring a goal from a penalty. On 16 November 2014, in a UEFA Euro 2016 qualifying match against Croatia at the San Siro in Milan, De Rossi earned his 100th cap in a 1–1 draw. On 6 September 2015, he scored from a penalty at the six-minute mark in Italy's 1–0 victory over Bulgaria in a Euro 2016 qualifying match in Palermo, and was later sent off during the match for a reactionary foul; this was his second red card with Italy, which made him Italy's most red carded player ever, alongside Giancarlo Antognoni and Franco Causio. On 31 May 2016, he was named to Conte's 23-man Italy squad for UEFA Euro 2016. On 27 June, De Rossi limped off the field in the eventual 2–0 victory round of 16 match up against Spain, which caused him to miss out on the quarter final match against Germany on 2 July, in an eventual 6–5 penalty shoot-out defeat. Many believed an unfit De Rossi would have come on just for the shoot-out, but when accused of refusing to take a kick he responded, "Do I look like the type who would refuse to step-up to the spot? ... Conte didn't ask me, and that's it. I hadn't even warmed-up during the game."