Kimbrel got his first call-up from the Gwinnett Braves on May 15, 2010, to replace the injured Jair Jurrjens on the roster. He was called up for the second time in his career on June 4, 2010, to replace Takashi Saito, who was placed on the 15-day DL. He earned his first major league save on September 19, 2010 against the New York Mets. Kimbrel's record for the 2010 season was 4–0, with one save and a 0.44 ERA in innings. He recorded 40 strikeouts and 16 walks. In the 2010 NLDS, he was the losing pitcher in Game 3 against the eventual World Series Champion San Francisco Giants.