Inadequate lodging resulted in the camp being moved away and designated as "Camp Cameron". On 15 June the regiment was ordered to proceed to Boston. Later it marched to Washington, D. C., then Georgetown where it established "Camp Banks". The regiment remained there until it was called to fight during First Battle of Bull Run. After retreating, it returned to Washington. In Bladensburg Muzzey was promoted to second lieutenant and mustered with 23rd Regiment Massachusetts Volunteer Infantry with which he fought at Roanoke Island and New Bern as part of Burnside's North Carolina Expedition. In New Bern he was tasked with provost duty. On 17 July he resigned commission and returned to Massachusetts.