Isis Holt (born 3 July 2001) is an Australian Paralympic athlete competing in T35 sprint events. She is affected by the condition cerebral palsy. Holt won gold medals in the 100 m and 200 m at the 2015 and 2017 World Para Athletics Championships. At the 2016 Rio Paralympics, she won two silver medals and a bronze medal. She has been selected for the 2020 Summer Paralympics, her second games.