At the outbreak of hostilities in September 1939 the regiment raised the "Val Cismon", "Val Piave", and "Val Cordevole" battalions from its reservists. The regiment and its six battalions were sent to the Western Alps to participate in the invasion of France. After the invasion the regiment and its three core battalions were again assigned to the 5th Alpine Division "Pusteria", while the three reserve battalions were disbanded on 31 October 1940. In November the "Pusteria" was sent to Albania to fight in the Greco-Italian War. Already in January 1941 the "Val Cismon" was raised again and sent to Albania, where Italian forces were suffering heavy casualties, including the commanding officer of the 7th Alpini Regiment Colonel Rodolfo Psaro, who was killed on 8 December 1940.