After Tobruk was placed under siege by Axis forces in April 1941, the regiment moved to the fortress at Mersa Matruh, Egypt, with four guns being deployed forward in an anti-tank role. In May, the regiment received new 25-pounders at Tel el Kebir, Egypt, from the 9th Division before the Allies launched the Syria–Lebanon Campaign and moved to Affula, Palestine. As part of the invasion of Syria and Lebanon held at the time by the Vichy French, the regiment supported the Australian 21st Brigade's advance along the coast. Crossing the border on 8 June, the 7th Battery, as part of the advance guard which overlooked the Litani River, fired the first artillery shots of the campaign, subsequently providing critical fire support which allowed a bridgehead to be secured. During the fighting that followed the regiment undertook anti-tank, direct-fire tasks, counter battery fire and came under enemy air and naval attack. At the conclusion of the campaign, the 2/4th remained in Syria undertaking garrison duty. During its time there the regiment was expanded to include a third battery, which was designated the 54th Battery.