The regiment was organized in Wheeling, Morgantown and Clarksburg in 1861 and consisted of 13 companies, plus an additional company that was attached for most of the war. Members were predominately recruited from Ohio and Pennsylvania and the western Virginia counties of Marshall, Monongalia, Harrison and Ohio. The regiment was often split during the first two years of the war, with detachments spending time guarding the Baltimore &amp; Ohio Railroad and hunting bushwhackers. During July 1863, ten companies of the regiment fought at the Battle of Gettysburg as part of a division.