The regiment fought in the Appomattox Campaign between 28 March and 9 April, during which it participated in the Union breakthrough at Petersburg on 2 April. The regiment prepared for the assault along with the rest of the division on the night of 30 March, but it was postponed until the morning of 2 April. Shortly after 03:00, it advanced from the camp and formed in a line of battle directly in front of Fort Sedgwick with its left on the Jerusalem Plank Road, the forward regiment of the brigade. Cox led the regiment through a hail of fire across the Confederate picket line and the quickly dismantled chevaux-de-frise to overrun the main Confederate line; the regimental colors were planted on the parapet of the fort commanding the road. The commingled regiments of the brigade then turned to the left and seized three other fortifications and captured five cannon, which were turned around against the Confederate troops.