On 27 June 2016, Juventus announced the signing of Alves on a two-year deal with the option of a third year. He made his Juventus debut on 20 August in a 2–1 home win over Fiorentina in Serie A. On 21 September, Alves scored his first goal with Juventus in a 4–0 home win over Cagliari, before opening his Champions League account with the club against Dinamo Zagreb six days later. On 27 November, he suffered a broken leg in Juventus' 3–1 defeat to Genoa. Alves made his return from injury as a substitute in a 1–0 "Derby d'Italia" win over Internazionale on 5 February 2017.