He began his Formula One career in 1999 as the test driver for Minardi. In late February , it was announced that he would be the teammate of Marc Gené in the race team. "I intend to learn over the first half of the season and then I feel I will have the confidence to perform well," Mazzacane told the press at the launch of the Minardi M02. His debut year in Formula One began with a broken gearbox at his first race, the 2000 Australian Grand Prix, followed by a 10th place in Brazil. He went on to outqualify Gené at Imola, a feat he later accomplished twice more. The German Grand Prix was a relative high point for him; after outqualifying his teammate, he finished 11th. His highlight in the spotlights this season was on a damp Indianapolis track when he famously overtook Mika Häkkinen, who was struggling after an early gamble on dry tyres. After running up to third without stopping, he ran over his pitcrew and dropped out of the race later. Nevertheless, he finished 11 of the 17 races that year and ranked third among drivers with the most kilometres raced.