Defensive coordinator Billy Davis retained Wilson and Antrel Rolle as the starting safety duo to begin the regular season. On December 14, 2009, Wilson recorded a season-high eight solo tackles, a career-high five pass deflections, and an interception during a 24–9 loss at the San Francisco 49ers in Week 14. On December 26, 2009, Wilson recorded four solo tackles, a pass deflection, an interception, and a sack during a 31–19 victory against the St. Louis Rams. His sack was the 20th of his career and earned him the recognition of being the 14th player to record 20 sacks and 20 interceptions in their career and become the a member of the 20–20 club. On December 29, 2009, Wilson was voted to the 2010 Pro Bowl, marking the third selection of his career. Wilson completed the 2009 season with 74 combined tackles (61 solo), 13 passes defensed, five interceptions, and two sacks in 16 games and 16 starts.