For the offensive in Palestine, the brigade was assigned to the Australian Mounted Division. Prior to their commitment to operations, the troopers were converted from cameleers and were issued swords and received cavalry training. Having been formed late in the war, the 5th Light Horse Brigade's involvement in combat operations was limited to the final offensive of the war, which began with the Battle of Megiddo in late September 1918. During this offensive, the brigade took part in the Battle of Sharon and Battle of Tulkarm, taking a large number of prisoners to the west of the Samaria Ranges. During operations to secure Damascus, the brigade took up positions overlooking the Barada Gorge, helping to cut off withdrawing Ottoman and German troops, and inflicting heavy casualties on them during an ambush on 30 September. The brigade then spent the night there in freezing conditions before elements entered Damascus on 1 October. For the remainder of the month, it was engaged in minor mopping up and patrol operations before joining the final stage of the campaign, which saw the brigade begin the advance towards Aleppo. The advance was short lived, as on 30 October the Ottomans surrendered, and the Armistice of Mudros came into effect, bringing an end to the fighting in the theatre.