Lesotho sent a delegation to compete at the 2016 Summer Paralympics in Rio de Janeiro, Brazil, from 7 to 18 September 2016. This was the fifth time the country competed in the Summer Paralympic Games after it made its debut sixteen years prior at the 2000 Sydney Paralympics. The delegation to Rio de Janeiro consisted of two athletes: sprinter Sello Mothebe and discus thrower Litsitso Khotele. Mothebe originally came third in the heats of the men's 200 metres T12 and the men's 400 metres T12 events but he was retroactively disqualified for testing positive for a banned substance. Khotele ranked tenth in the women's discus throw F43–44 competition with a throw of 19.91 metres.