Since its release, Third Day has performed "Born Again" in concert. At WinterJam 2010, Third Day performed the song with Dawn Michele of Fireflight. On the opening night of the Make a Difference Tour, Third Day performed "Born Again" as part of their setlist. At a concert as part of their Make Your Move Tour in Wilkes Barre, Pennsylvania on November 6, 2011, the band performed the song during an acoustic set; the band took requests, and "Born Again" was one of the songs requested. Third Day also performed the song at a concert in Joplin, Missouri on March 20, 2011; following the devastating tornado that struck Joplin on May 22, 2011, Third Day released the entire concert as a download, with all proceeds going to tornado relief for Joplin.