On 2 June 2014, Biglia was called up for the 2014 FIFA World Cup in Brazil. A late substitute in every match up to that point, he was an undisputed starter from the quarter-finals onward, as his nation lost 0–1 to Germany in the final. Biglia also appeared as a starter in five of Argentina's six 2015 Copa América matches, including the final where "La Albiceleste" lost in a penalty shootout to hosts Chile.