After the outbreak of the Second Boer War in October 1899, the regiment was sent to South Africa. It formed part of General French′s force in the operations around Colesberg, and afterwards one squadron took part in the relief of Kimberley in February 1900. The squadrons of the regiment then rejoined, and formed part of the cavalry brigade which served in all the engagements in the general advance on Pretoria, one of the Boer capitals, which was captured on 5 June 1900. They took part in the battles of Diamond Hill (June 1900) and Belfast (August 1900), and in the following cavalry advance on Barberton in the Eastern Transvaal. The regiment subsequently joined a column under the command of Colonel Michael Rimington in the Orange River Colony, taking part in miscellaneous raids and drives there throughout the last year of the war, which ended with the Peace of Vereeniging on 31 May 1902. Following the end of the war, 257 officers and men of the regiment left Cape Town on the SS "Orissa", which arrived at Southampton in late October 1902, and another 138 men arrived at Queenstown the following month on the SS "Orient". After their return, the regiment was stationed at Curragh.