The RML 16 cm No. 3 was used in combat during the Battle of Shimonoseki Straits, where both "Djambi" and "Metalen Kruis" had the gun on board. "Djambi" fired 54 shot from the RML 16 cm No. 3 during the main fight. Her Captain reported that the RML 16 cm No. 3 did well as regards range and accuracy, but that most s (a mechanism to control recoil, consisting of a heavy rope from the bulwark through a ring on the bottom of the gun) were broken, but repaired. From "Metalen Kruis" 60 shot were fired by the RML 16 cm No. 3. Her commander was also very satisfied as regards range and accuracy, but also reported 7 s having to be renewed. He thought that in time, the gun would not stand up to continuous firing.