The squadron was reformed in March 1937 again as No. 80 Squadron, now equipped with Gloster Gauntlet fighter. However, by now the Gauntlet was considered by many to be outdated, and as a result the Gauntlets were replaced by the Gloster Gladiator just two months later. In 1938, the squadron again returned to Egypt as an 'air defence unit'. After Italy's declaration of war on Britain on 10 June 1940, No. 80 Squadron was moved to the Egyptian-Libyan border but was subsequently one of the units sent to aid the Greeks during the Greco-Italian War, initially flying Gladiators and then re-equipping with the Hawker Hurricane from February 1941. The Squadron lost most of its aircraft during the Greek and Crete actions and reformed at RAF Aqir in Palestine in May 1941 before deploying detachments to Nicosia in Cyprus and 'A' Flight to Haifa. The Squadron moved totally to Cyprus in July 1941, before returning to Syria the next month, and then joining the fighting in North Africa two months later. During the Battle of El Alamein it was responsible for defending communications lines. It remained in that area until early 1944, when it returned to Britain to prepare for Operation Overlord (the Allied invasion of Europe). It was equipped with the Spitfire IX F operating from RAF Detling in Air Defence of Great Britain (ADGB), though under the operational control of RAF Second Tactical Air Force (2nd TAF). When 2nd TAF began moving to Normandy after D-Day, the squadron remained in ADGB, re-equipping with Hawker Tempest aircraft on anti-V-1 flying bomb duties as part of Operation Diver. After this threat diminished, No. 80 Squadron moved on to the continent and resumed a fighter role until the end of the war in Europe.