The Greeks established colonies in the coastal towns of Saguntum and Dénia beginning in the 5th century BC, where they traded and mixed with the local Iberian populations. After the end of the First Punic War between Carthage and Rome in 241 BC, which established their limits of influence in the Ebro river, the Carthaginians occupied the whole region. The dispute over the hegemony of Saguntum, a Hellenized Iberian coastal city with diplomatic contacts with Rome, destroyed by Hannibal in 219 BC, ignited the Second Punic War, which ended with the incorporation of the region to the Roman Empire.