Soukalová began her biathlon career in 2005. In 2007 Soukalová became a member of the national team, and in 2008 she took part in the Biathlon Junior World Championship. Her first experience at the international level was not very good: Soukalová's shooting accuracy and ski speed were at that time not as strong as many of her peers, and her best result at the 2008 Junior Worlds was a 22nd place. Nevertheless, her coaches gave her a chance to take part in further international competitions, including the IBU Cup in the &lt;a href="2008%E2%80%9309%20Biathlon%20IBU%20Cup"&gt;2008/09 season&lt;/a&gt;. Her performances improved: Soukalová, despite some difficulties in shooting, became better than Olga Vilukhina and helped the Czech women's relay team to win a gold at the 2009 Junior World Championships.