In February 2011, Biglia was called up again, a decision that surprised him. Unlike his first call up, his first match for Argentina, in a friendly match against Portugal, which saw them win 2–1. During the same year, Biglia was included by manager Sergio Batista ahead of the Copa América tournament despite an injury, he sustained at Anderlecht, could threaten his chance to play in the tournament. At Copa América tournament, Biglia went on to make two brief substitute appearances as the hosts lost to Uruguay in the quarter-finals on a penalty shootout.