Neuner went into her first Winter Olympics in Vancouver with the declared aim of winning a gold medal. On 13 February 2010, she participated in the opening sprint, which was contested at Whistler Olympic Park in rainy conditions. With one shooting error, Neuner claimed the silver medal, finishing 1.5 seconds behind Slovakia's Anastasiya Kuzmina. She uncharacteristically lost five seconds against the unheralded Slovak on the cross-country course, which led to speculation of inferior ski preparation in the German media. Three days later, Neuner won gold in the subsequent pursuit race. Despite missing two targets in the standing position, she beat sprint winner Kuzmina by 12.3 seconds. In her third Olympic event, she finished tenth in the individual. She had three shooting mistakes and said it had been difficult for her to immediately get her concentration back after winning her first gold medal. On 21 February 2010, Neuner claimed her second gold of the Games in the mass start. After missing two targets, she had been trailing by as much as 29 seconds, but she pushed the pace and a clean final standing shoot allowed her to overtake Russia's Olga Zaitseva on the last lap. After the race, Neuner made the announcement not to participate in the relay, citing mental exhaustion and her desire to give all of her team mates the chance to win a medal. Her withdrawal allowed her friend Martina Beck a start in her last Olympic Games. Neuner was Germany's most successful athlete in Vancouver and was chosen to carry the German flag at the closing ceremony.