Born of a noble family, Tsulukidze was educated at Elisavetgrad military pro-gymnasium and Tiflis infantry junkers' school. Since 1876, he served in the Imperial Russian army. During World War I, he was promoted to the rank of major-general in 1915 and commanded a brigade in the 5th rifle division, then the 174th Infantry Division and finally the 67th Infantry Division in the years 1916–1917. On January 7, 1916, Giorgi Tsulukidze was awarded the Order of St. George (Fourth Degree). He then served in the military of a newly independent Democratic Republic of Georgia after whose fall to the Soviets (1921) he was involved in an underground independence movement. Tsulukidze was arrested by Cheka along with his associates and shot at the outskirts of Tbilisi on May 19, 1923.