The first combat use of the FAB-5000 took place on the night of 28 April 1943, when coastal fortifications at Königsberg were hit. The Pe-8 bomber that released the bomb from an altitude of 5,800 m was shaken by the shockwave of the explosion. On 19 July 1943, during the battle of Kursk, two Pe-8 dropped two bombs on a railroad yard near Orel, ripping apart a 100 m section of the railway and obliterating dozens of railcars and German military vehicles. Railroads and fuel depots had already been hit around Orel with one bomb on 4 June and with two bombs on 3 July. Two attacks were carried out on advancing German troops on 12 July, but further tactical use was suspended to avoid the risk of friendly fire. Soviet sources also claim that two buildings occupied by the Gestapo and the Belarusian Auxiliary Police were demolished by two FAB-5000 bombs at the city of Mogilev, Belarus, apparently on 26 May 1943. On 7 February 1944, another two FAB-5000 bombs were dropped on Helsinki, in the course of the 1944 Great Raids. Soviet sources claim that railway workshops and a cable factory were destroyed. A couple of days later, two more bombs fell on Finland's capital. The last FAB-5000 was dropped on the railway station of , Ukraine, on 9 March 1944, during the Soviet offensive on Kamenets-Podolsky pocket, halting all railroad traffic for several days.