In 1889, Rennotte attended the World Fair in Paris and after briefly returning to Brazil, left in June for the United States. She enrolled in the Woman's Medical College of Pennsylvania in Philadelphia to study medicine, having received scholarship funds authorized by Prudente Morais Barros, governor-elect of the state of São Paulo. That December a legal change gave citizenship to any foreigners who were permanently residing in Brazil. She graduated in 1892, becoming the first woman from São Paulo to earn a medical degree. Between 1893 and 1895, Rennotte studied at the Hôtel-Dieu Hospital in Paris, completing her residency and specialization in obstetrics and gynaecology with studies in neonatology, as well as skin conditions and sexually-transmitted diseases.