In July 2016, Lyles went to the U.S. Olympic Trials to compete for spots on the Olympic team in the 100 m and the 200 m. He failed to advance from his first 100 m heat, but in the 200 m he won his semi-final and then placed fourth in the final with a time of 20.09 s, breaking a 31-year-old national high school record. Though he didn't qualify for the 2016 Summer Olympics, he qualified for the 2016 World U20 Championships where he was a double gold medalist, taking the 100 m and relay titles.