In 2016, as a freshman for The University of Texas at El Paso (UTEP), Amusan became the second athlete for the university to be named C-USA Female Track athlete of the Year since UTEP joined C-USA. She was the gold medallist in both the 100 mH and the 200 m. She also claimed a silver in the long jump at the C-USA Championships. Amusan first broke the 13 s barrier in the hurdles with a time of 12.83 s at the El Paso UTEP Invitational. This eclipsed Kim Turner's 100 mH UTEP record which had stood for 33 years. She was runner-up at the 2016 NCAA Outdoor Championships in the 100 mH. She ran a windy 12.79 s behind Kentucky's Jasmine Camacho-Quinn. Amusan also competed at the 2016 World Junior Championships in Bydgoszcz. Despite running her second fastest time ever, she placed fifth in the final. She went on to represent Nigeria at the Rio Olympic Games, reaching the semifinals of the 100 mH.