In August 2002, Ohno was selected Japan U-20 national team for 2002 U-19 World Championship. She played 4 games and scored 3 goals. On 12 January 2003, she debuted for Japan national team against United States. Her first major tournament was the 2006 Asian Cup, where Japan placed fourth. The following year she played in the 2007 World Cup, but Japan fell in the group stage. She also competed at the 2008 Summer Olympics, and the 2011 World Cup, where she scored a goal in the group stage match against Mexico as Japan won the championship. Ohno started the final against the United States. She then competed for the Japanese silver medal-winning team at the 2012 Summer Olympics. At 2015 World Cup, Japan won 2nd position. At 2016 AFC Women's Olympic Qualifying Tournament, following Japan's failure to qualify for the 2016 Summer Olympics. This tournament became her last match for Japan. She played 139 games and scored 40 goals for Japan until 2016.