The regiment arrived in South Africa in December 1899 and took part in the Battle of Colenso during the Second Boer War. It formed part of Colonel Burn-Murdoch’s Brigade and had a minor part in the Relief of Ladysmith in February 1900. The regiment stayed in South Africa throughout the hostilities, which ended with the Peace of Vereeniging on 31 May 1902. Following the end of the war, 556 officers and men of the regiment left South Africa on the SS "City of Vienna", which arrived at Southampton in October 1902.