John Francis Colburn (September 30, 1859 – March 16, 1920) was a businessman and politician of the Kingdom of Hawaii. He served as the last Minister of the Interior of Queen Liliuokalani. Even though he was part Hawaiian ancestry on his maternal side, Colburn was a key figure in the overthrow of the Hawaiian monarchy and was a proponent of annexation to the United States. Colburn was the treasurer of the estate of Queen Kapiolani.