Van Bronckhorst's final match for the Netherlands and as a professional footballer came in the World Cup final against Spain. He was substituted in the 105th minute for Edson Braafheid with the score 0–0, only for Andrés Iniesta to condemn the Dutch to a defeat, scoring the only goal of the match in the 116th minute. After ending the tournament as runners-up, Van Bronckhorst stated he was proud of what the team had achieved.