The regiment served in western Virginia for most of the balance of the year. In 1862, the regiment was sent into Virginia's Shenandoah Valley, where it suffered significant casualties during First Battle of Kernstown in March. At the subsequent Battle of Port Republic on 9 June, the Buckeyes lost 244 killed, wounded and prisoners. Its ranks much depleted from the Valley Campaign, the regiment served in the defenses of Washington, D.C. under John Pope and participated in the Northern Virginia Campaign.