An engagement occurred at nearby Neuville on 24 November and the ambulance sent men into the field to tend to the wounded of this and subsequent skirmishes of 25 and 26 November. The ambulance managed to secure space for a 300-bed hospital at the Church of St. Euverte. The Prussians commenced the Second Battle of Orléans on 3 December and large numbers of wounded were soon brought back to the ambulance. A field unit was sent out on 4 December but the speed of the French retreat meant that a field hospital could not be established. Prussian forces retook Orléans on the night of 4 December, bringing the ambulance under their orders once again. A mobile unit was provided for the Battle of Beaugency on 7 December and returned to the city on 10 December with wagons loaded with wounded men. A number of the ambulance's men were arrested by the Prussian authorities on suspicion of espionage whilst procuring medical supplies at Étampes but were shortly thereafter released. The ambulance remained in Orléans until the armistice was signed and disbanded in early March with its equipment being handed over to the French in Paris.