Uceny won the national titles in the 1500 m at the 2012 US track and field Olympic Trials, qualifying for her first Olympics, alongside Shannon Rowbury, and Jenny Simpson. At the London Games, Uceny and Rowbury made it to the final, but a mid-race clash with Ekaterina Kostetskaya meant she fell hard onto the track as the pack moved on, injuring her back and right side. She did not finish the race and left the stadium in tears.