With the retirement of Ruud van Nistelrooy, Huntelaar became new Dutch national team coach Bert van Marwijk's first choice striker for the 2010 World Cup qualification match against Iceland in October 2008, and scored in a 2–0 victory. Huntelaar was included in the preliminary squad for the 2010 World Cup in South Africa. On 27 May 2010, Van Marwijk announced that the player would be part of the final squad of 23 participating in the competition. On 24 June, he replaced goalscorer Robin van Persie in the 58th minute against Cameroon at the Green Point Stadium in Cape Town and scored the winning goal in the 83rd minute in a 2–1 win, taking his goal tally to 16 in 34 games for the Netherlands. Huntelaar also came on as a substitute for Van Persie in the round of 16 game against Slovakia and in the quarter-final against Brazil. He was an unused substitute in the final against Spain as the Netherlands lost their third World Cup final 0–1 due to a late goal by Andrés Iniesta.