The 16th Punjab Regiment was formed in 1922 by amalgamation of the 30th, 31st, 33rd and 46th Punjabis, and 9th Bhopal Infantry. Except for the 46th Punjabis, who were raised in 1900, the rest were raised during the upheaval of the Indian Mutiny in 1857-59. The 30th and 31st Punjabis were raised in 1857, as the 22nd Regiment of Punjab Infantry and Van Cortlandt's Levy respectively. The 33rd Punjabis were also raised in 1857, as the Allahabad Levy, while the 9th Bhopal Infantry was raised in 1859, as the Bhopal Levy from the remnants of loyal elements of the Bhopal Contingent. The 30th and 31st Punjabis served in the Bhutan War of 1864-66 and all the battalions saw service on the North West Frontier of India. The 30th and 31st Punjabis along with the 9th Bhopal took part in the Second Afghan War of 1878-80, while the 33rd Punjabis served in the Third Anglo-Burmese War of 1885-87.