On 3 January 2013, Peluso joined Juventus on a six-month loan, with a view to a permanent transfer after the period. He spent the second half of the 2012–13 campaign on loan with the Bianconeri, and his performances earned him a permanent transfer and a contract until June 2017, as Juventus captured the league title. In June 2013, Juventus announced that they had completed the signing of Federico Peluso from Atalanta for a transfer fee of €4.8 million. After the end of the 2013–14 season, Serie A side Sassuolo announced that they acquired Peluso outright, as part of the deal that brought Luca Marrone back to Juventus.