Stosur lost her second-round match against Arantxa Rus at Wimbledon. In the London 2012 Olympics, Stosur lost in the first round to Carla Suárez Navarro. Stosur won the first set but lost the second and then the third set 8–10. Stosur played mixed doubles with former world No. 1, Lleyton Hewitt. They reached the quarterfinals together, but lost to crowd favorites Andy Murray and Laura Robson, who were competing for Great Britain.