From 1526 to 1687 Syrmia was part of the Ottoman Empire within the Budin Eyalet. At the end of this period the Great Migrations of the Serbs occurred resulting in Serb settlement in the region which was at the time part of Habsburg Monarchy. Mirkovci was established in 18th century within the Slavonian Military Frontier, which acted as the "cordon sanitaire" against incursions from the Ottoman Empire in which Serb colonists were defending the Monarchy in return for land-grants and internal self-administration granted in "Statuta Valachorum". Soldiers from Mirkovci fought in Austro-Turkish War (1788–1791), Napoleonic Wars, various battlefields during the Revolutions of 1848 under the command of Josip Jelačić and Joseph Radetzky von Radetz, the Second Italian War of Independence and the Third Italian War of Independence. Serbian Orthodox Church of St. Nicholas was constructed in period between 1804 and 1813. The village remained a part of the Slavonian Military Frontier until 1881 when the former was disestablished. Between 1881 and the end of Austro-Hungarian rule the village was part of the Kingdom of Croatia-Slavonia and its Syrmia County.