During winter 2016, Tamberi won every contest he participated at. He won in Banska Bystrica with 2.35 m, a new Italian indoor record, equalled by Marco Fassinotti in the same event. He won in Trinec after clearing 2.33 m. At the 2016 High Jump Moravia Tour, he recorded a jump of 2.38 m, enough to beat Chris Baker of Great Britain and Kyriakos Ioannou of Cyprus, and which gave him the Italian indoor high jump record. He won a gold medal at the World Indoor Championships in Portland in March 2016 with a jump of 2.36 m.