Ledecky's first event in the 2016 Summer Olympics was the 4×100-meter freestyle relay, swimming the anchor leg for the United States in both the prelims and final. Ledecky recorded a split of 52.64 in the heats. In the final, she joined Simone Manuel, Abbey Weitzeil, and Dana Vollmer and recorded a split of 52.79, helping the team earn a silver medal (behind Australia) with a time of 3:31.89, a new national record for the United States.