This provoked the First War. The Bourbons, with English support and led by Louis I de Bourbon, Prince de Condé, and Admiral Coligny, began to seize and garrison strategic towns along the Loire. The Battle of Dreux and the battle of Orléans were the first major engagements of the conflict. In February 1563, at Orléans, Francis, Duke of Guise was assassinated, and Catherine's fears that the war might drag on led her to mediate a truce and the Edict of Amboise (1563), which again provided for a controlled religious toleration of Protestant worship.