As a component of Perry's Brigade, the 2nd Florida continued to serve through the Army of Northern Virginia's campaigns. Through the remainder of 1862, the regiment participated in the Battles of Second Manassas in August, Sharpsburg in September, and Fredericksburg in December. In May 1863, the 2nd Florida fought in the Battle of Chancellorsville. As the Army of Northern Virginia began preparations for its invasion of Pennsylvania, Brig. Gen. Perry fell ill with typhoid and command of the Florida Brigade devolved to Colonel David Lang of the 8th. The Florida Brigade was engaged at the Battle of Gettysburg and participated in Pickett's Charge on the battle's third and final day.