Son of Admiral Robert Wilson Shufeldt and Sarah Shufeldt, he was born in New York in 1850. After a school education in the United States and Havana, he joined as a Captain's clerk on the US Gunboat which was under the command of his father. In 1872 he joined Cornell University to study medicine and obtained a degree in 1876 from Columbian, Washington DC. He joined the Medical Department of the Army as a Lieutenant and was posted to Fort McHenry. He later worked as a surgeon in the campaign against the Sioux Indians. He retired in 1891 from the army as a Captain but was readmitted and posted on duty in the Army Medical Museum as a curator in 1882. He retired on January 9, 1919 as a surgeon. From 1884 to 1888 he was stationed at Fort Wingate in New Mexico, collecting in the Zuni Mountains and nearby regions. He was a lifelong member of the Democratic Party and a supporter of Woodrow Wilson.