At the 2008 Summer Olympics in Beijing, Almirao competed again for the second time in individual and relay sprint events. For her first event, 400 metres, Almirao ran in the fourth heat against six other athletes, including Great Britain's Christine Ohuruogu, who eventually became an Olympic champion in the final. She finished the race again in fifth place by more than two seconds behind Ohuruogu, with a time of 53.26. Few days later, Almirao reunited with her teammates Lucimar Teodoro, Josiane Tito, and Emmily Pinheiro for the women's 4 × 400 m relay. She ran on the starting leg of the second heat, with an individual-split time of 53.49 seconds. Almirao and her team finished the relay in sixth place for a total time of 3:30.10, failing to advance into the final.