On the February 13 episode of "Raw", Flair dropped the title to Bayley in the main event. She later failed to regain the title in a rematch against Bayley at Fastlane on March 5, marking her first pay-per-view singles match loss after 16 singles victories. Also in March, Flair ended her association with Brooke and defeated her in a singles match. At WrestleMania 33 on April 2, Flair competed in a four-way elimination match against Bayley, Banks, and Nia Jax, being the last woman eliminated by eventual winner Bayley. On the April 10 episode of "Raw", Flair was defeated by Jax in her final match for the brand.