The 14th Indiana fought at the Battle of Antietam, the Second Battle of Fredericksburg, the Battle of Chancellorsville, and at the Battle of Gettysburg. At Gettysburg, the 14th Indiana helped secure Cemetery Hill. From August 16 to September 6, 1863, the regiment was detached for duty in New York City to help prevent further violence following the New York City draft riots of July 1863. After its return to active duty, the regiment fought in the Bristoe Campaign and the Mine Run Campaign, as well as several major battles, including the Battle of the Wilderness and the Battle of Spotsylvania Court House. The Battle of Cold Harbor was the regiment's final last engagement before it left the front on June 6, 1864. Regimental members who had completed their military served mustered out on June 20, 1864, at Indianapolis; its veterans who had re-enlisted and its remaining recruits were transferred to the 20th Regiment Indiana Infantry.