Bruscolotti subsequently moved to S.S.C. Napoli in 1972, where he spent most of his career, making over 500 appearances for the team during his sixteen seasons in Naples; he made his Serie A debut with Napoli during the 1972–73 season, in a 1–0 win over Ternana on 24 September 1972. Bruscolotti became a symbol and a legend of the club, and played a key role in helping Napoli to their first ever Serie A title in 1987; he was also the team's captain from 1978 until 1984, when he handed over the armband to Diego Maradona. He retired at the age of 37 in 1988; he holds the record for most Serie A appearances for Napoli (387), and has made the second most appearances in all league competitions for the club. He also made 96 appearances for Napoli in the Coppa Italia, and 28 in European competitions. Bruscolotti also scored 11 goals for Napoli throughout his career; one of his most notable goals came in a 1–0 home victory over Anderlecht in the return leg of the 1976–77 European Cup Winners' Cup semi-finals, although Napoli were eliminated by the eventual champions on aggregate. His most important goal for the club came in the second leg of the 1976 Anglo-Italian League Cup final, which saw Napoli triumph over Southampton. In addition to these titles, Bruscolotti also won two Coppa Italia titles with Napoli.