Throssell again represented Australia at the 2020 Summer Olympics, held in Tokyo in 2021, where she swam in the 100m and 200m butterfly events, coming in 8th place in the final of the 200m event. She won a gold medal in the Women's 4 x 100 metre medley relay and a bronze medal in the Mixed 4 x 100 metre medley relay after swimming the butterfly leg in the heats for both events. She also won a bronze medal in the Women's 4 x 200 metre freestyle relay after swimming in the heats of that event.