On 21 September 1953 the squadron was reformed at RAF Scampton as a bomber unit with the English Electric Canberra as part of a four-squadron Canberra wing. The Scampton wing was dispersed in 1955 to allow Scampton to be redeveloped as a base for V-bombers, with 21 Squadron moving to RAF Waddington in May 1955. It was involved in many overseas detachments, and was part of the forces involved in the Suez operation of 1956. The squadron was disbanded on 30 June 1957 at Waddington.