On January 2, 2017, Bayley defeated Jax to become the number one contender for Flair's Raw Women's Championship. She challenged Flair at the Royal Rumble but was unsuccessful in capturing the title. She earned a rematch after she, Cesaro and Sheamus defeated Flair, Gallows and Anderson the next night on "Raw", with Bayley pinning Flair. Two weeks later on the February 13 episode, Bayley defeated Flair in the main event to win the Raw Women's Championship after an assist from Banks. At Fastlane, Bayley retained the title against Charlotte thanks to a distraction from Banks and in turn gave Charlotte her first pay-per-view loss. At WrestleMania 33 in Bayley's WrestleMania debut, she retained the title against Charlotte, Banks and Jax in a fatal four-way elimination match. On April 30, Bayley dropped the title to Bliss at Payback, ending her reign at 76 days. She failed to regain the title in a kendo stick on a pole match at Extreme Rules on June 4. In July 2017, Bayley again became the number one contender for the championship and was slated to face Bliss at SummerSlam on August 20, but she suffered a separated shoulder injury which left her unable to perform. Bayley returned from her injury on the September 18 episode of "Raw" to help Banks fend off Bliss and Jax. At No Mercy six days later, she lost a fatal five-way match for the Raw Women's Championship.