Originally constituted as the 2d Pursuit Squadron on 20 November 1940, the squadron was activated on 15 January 1941. It served in World War II with the 52d Pursuit Group, and during that period flew the Curtis P-40 Warhawk and Bell P-39 Airacobra. The 2d also flew combat missions in the Supermarine Spitfire and North American P-51 Mustang in the European and Mediterranean Theaters, serving specifically in air campaigns in Europe, Algeria, French Morocco, Tunisia, Sicily, Naples-Foggia, Rome-Arno, Normandy, Northern France, Southern France, north Apennines, Rhineland, Central Europe, Po Valley, and performed air combat. The unit received two Distinguished Unit Citations for operations in Germany and Romania in 1944. Following World War II, the squadron was inactivated on 7 November 1945 at Drew Field, Florida.