Biglia's first senior international call-up for Argentina was in 2011 and he previously played for the Argentina U20 national team, who won the 2005 FIFA World Youth Championship. He represented his nation at two FIFA World Cups, reaching the final of the 2014 edition of the tournament, and in three Copa América tournaments, winning runners-up medals in the 2015 and 2016 editions of the tournament. He retired from international football after the 2018 FIFA World Cup, with 58 caps and 1 goal.