The regiment would remain in Annapolis until 3 January 1862 when it moved with Burnside's expedition into North Carolina where it participated during the Battle of Hatteras Inlet Batteries and later the Battle of Roanoke Island between 7 January and 8 February. Although a detachment only served during the battle, this would be the sole major action of the regiment. After the expedition, the regiment remained on duty at Fort Monroe, Virginia, later, Annapolis, Maryland, and later the District of Columbia until March.