From its initial formation in August 1861, the regiment became known as one of Indiana’s most highly disciplined regiments because of its infantry maneuvers, which were based on Prussian infantry tactics and bugle calls, and the general good health of its men. During 1861 and 1862 the regiment's major engagements included the Battle of Rowlett's Station, the Battle of Shiloh, the Siege of Corinth, and the Battle of Stones River. In 1863 the 32nd Indiana participated in three major campaigns: the Tullahoma Campaign, the Chickamauga Campaign, and the Chattanooga Campaign. In 1864 the regiment joined the Atlanta Campaign and fought in battles at Resaca, Adairsville, New Hope Church, Dallas, Pickett's Mill, Marietta, Kennesaw Mountain, Peachtree Creek, Atlanta, Jonesborough, and Lovejoy's Station. The 32nd Indiana completed its service in Texas, and mustered out on December 4, 1865.