From December 1863 until March 1864, Battery E took part in operations in East Tennessee. The unit traveled to Annapolis, Maryland before participating in the Overland Campaign on 4 May–7 June. At the beginning of the campaign, the battery was commanded by Lieutenant James S. Dudley and was one of six batteries of the Artillery Reserve of IX Corps under Ambrose Burnside. Battery E fought at the Battle of the Wilderness on 5–7 May 1864, the Battle of Spotsylvania on 8–21 May, the Battle of Totopotomoy Creek on May 28–31 May, and the Battle of Cold Harbor on 1–7 June. At Cold Harbor, Lieutenant Samuel B. McIntire commanded the battery. Shortly afterward, Battery E was ordered to return to Washington, D.C. where it served as a garrison until October 1865.