In addition to the minor battles of Reams' Station, Battle of Deep Bottom, Strawberry Plains, and Hatcher's Run, the regiment was daily engaged during the siege of Petersburg—from 16 July 1864, to 1 April 1865—on the picket line and in the trenches with frequent and continuous losses of men from wounds or by sickness caused by constant exposure. After the battle at Reams' Station, Capt. Nelson Penfield was placed in command, the colonel having been placed previously in charge of the brigade.