The regiment's major engagements included the Battle of Rowlett's Station, the Battle of Shiloh, and Battle of Stones River during 1861 and 1862. The 32nd Indiana also participated in the Tullahoma Campaign, the Chickamauga Campaign, and the Chattanooga Campaign in 1863, and in numerous battles during the Atlanta Campaign in 1864. After its first group of three-year enlisted men mustered out at Indianapolis on September 7, 1864, the remaining troops in the regiment fought at the Battle of Jonesborough and the Battle of Lovejoy's Station. A reorganized 32nd Indiana, which included a battalion of four companies, was attached to the Department of Texas and served in Texas until its remaining men mustered out of service on December 4, 1865.