On the 26th of September, the regiment moved from Fort Monroe to Fort Hatteras, recently taken from the Confederates during the Battle of Hatteras Inlet Batteries, on Hatteras Island in preparation for an assault against Confederate troops defending further up the inlet. 500 men in 7 companies were deployed to Chicamicomico Beach, Hatteras Island, on September 29, 1861. Their main objective was to dislodge an enemy force from Roanoke Island. The commanding officer of Fort Hatteras was in such a haste to have this objective accomplished that the men landed at Chicamicomico Beach without supplies or tents. Regimental Quartermaster Isaac W. Hart and a small detail arrived with proper supplies on October 1. However, the gunboat carrying Hart and the supplies was attacked by three Confederate gunboats shortly after Hart began unloading the supplies that afternoon. Hart and 47 men were taken prisoner, and the supplies and equipment were captured by the Confederate gunboats, who had kept the Regiment under suppressing fire to prevent any interference from land