2005 wasn't a fantastic for Groot with a 4th place in the 4×100 m freestyle and 9th in the 50 m butterfly during the 2005 World Aquatics Championships in Montreal, Quebec, Canada. At the end of the year she won the gold medal for the third time in a row in the 4×50 m freestyle at the European Short Course Swimming Championships 2005. 2006 started off with the 2006 FINA Short Course World Championships in Shanghai. Together with Inge Dekker, Hinkelien Schreuder and Marleen Veldhuis, Groot set a world record and won a gold medal in the 4×100 m freestyle relay in a time of 3:33.32. In the summer she took part in the 2006 European Aquatics Championships in Budapest, Hungary where she added a silver medal, in the 4×100 m freestyle with Dekker, Veldhuis and Ranomi Kromowidjojo, and a bronze medal, fourth time in her career, in the 50 m butterfly to her impressive collection of medals. In Helsinki she won a silver medal in the 4×50 m freestyle at the European Short Course Swimming Championships 2006. Her next big tournament was the 2007 World Aquatics Championships in Melbourne, Australia where she was swum out of the 4×100 m freestyle team by teammate Femke Heemskerk while being a regular team member in the finals for so many years. Individually she ended 13th and 20th in the 50 m and 100 m butterfly making it a disappointing tournament for Groot. A fifth spot at the European Short Course Swimming Championships 2007 was her best result of a disappointing year.