Mazoon Al-Alawi entered the 2016 Olympics as a wild card. Her entry time of 12.04 seconds was 0.72 seconds slower than the Olympic qualifying time for the women's 100 metres. On 12 August, Al-Alawi finished third in the second heat of the preliminary round with a time of 12.30 seconds, which qualified her as one of the two fastest remaining runners following the top two finishers of all of the heats. In the subsequent round (held the same day) Al-Alawi came in eighth in the seventh heat. She did not automatically advance to the next round as she did not finish in the top two of her heat and, with a time of 12.43 seconds, she was slower than the next eight qualifiers and so she did not progress past the heats. Al-Alawi finished 62nd overall out of 80 runners.