On September 26, the regiment moved from Fort Monroe to Fort Hatteras, recently taken from the Confederates during the Battle of Hatteras Inlet Batteries, on Hatteras Island in preparation for an assault against Confederate troops defending further up the inlet. Gilbreath was deployed with 500 men in 7 companies to Chicamicomico Beach, Hatteras Island, on September 29, 1861. Their main objective was to dislodge an enemy force from Roanoke Island. The commanding officer of Fort Hatteras was in such a haste to have this objective accomplished that the men landed at Chicamicomico Beach without supplies or tents. Regimental Quartermaster Isaac W. Hart and a small detail arrived with proper supplies on October 1. However, the gunboat carrying Hart and the supplies was attacked by three Confederate gunboats shortly after Hart began unloading the supplies that afternoon. Hart and 47 men were taken prisoner, and the supplies to be delivered to Gilbreath were taken as the spoils if war by the Confederates. The commanding officer of Fort Hatteras sent supplies on October 3, which were very welcomed by the men.