The 53rd fought during the Seven Days Battles, which began on June 27, when Confederate forces attacked the V Corps near Gaines' Mill. Richardson's division was rushed forward to the corps' assistance, crossing the Chickahominy and came under fire of Confederate artillery and infantry. Forming in line of battle, the division covered the Union withdrawal and at midnight re-crossed the Chickahominy. The regiment fought again at the Battle of Savage's Station on June 29 in order to protect Union supplies and wounded near the railroad station. After a short fight, the Confederates withdrew and the retreat was resumed at midnight, French's brigade acting as rearguard for the corps. At daylight the regiment reached White Oak Creek and rejoined the corps. The regiment was assisting in the destruction to a bridge when Confederate skirmishers appeared and opened fire on the Union troops. A number of Confederate batteries opened fire on the Union lines, though did little damage. Although not seriously engaged, the regiment lost several men wounded. The regiment withdrew with its brigade soon after midnight and marched to Malvern Hill, arriving on the morning of July 1. Though constantly under artillery fire throughout the Battle of Malvern Hill, no further losses were inflicted on the regiment.