On 4 July 2012, Lúcio signed a two-year contract with Serie A titleholders Juventus, adding him to the list of players to play for both Inter and Juventus. Although he was initially used as a starter in the club's three-man defence, featuring in Juventus's 4–2 victory over Napoli in the 2012 Supercoppa Italiana on 11 August, after struggling with injuries and producing some inconsistent performances, he was soon left out of the first-team in favour of the defensive trio of Giorgio Chiellini, Leonardo Bonucci and Andrea Barzagli. In total, he made only four appearances in all competitions during his next six months with the club: one in Serie A, two in the Champions League, and one in the Supercoppa Italiana. Due to this lack of playing time, Lúcio left Juventus on 17 December 2012, after his contract was terminated by mutual consent.