标题：袁凌
Classes
Abt301 - Writing and Presentation in Life Sciences; Credits - 2
This class is intended to refine skills in communicating and evaluating science-based knowledge. Specific focus is given to the agricultural, biological, and bio-medical sciences. One major goal is to facilitate the writing of an Agricultural Biotechnology Independent Research Proposal. In the process, students will learn how to evaluate relevant literature, think about science and the scientific process, and communicate scientific results and implications. This course should further the students' experience with science, enhance their appreciation for the scientific process, and what it means to conduct scientific research..
Instructors: Ling Yuan
Pls609 - Plant Biochemistry; Credits - 3
The course will consider the chemical constituents of plants (with emphasis on biologically or nutritionally significant compounds unique to plants), their biosynthesis, contribution to key metabolic and defense processes and the regulation of their synthesis. Included will be discussions of photosynthesis, carbohydrates, lipids, isoprenoids and phenylpropanoids, nitrogen fixation, nitrogen and sulfur reduction and assimilation, alkaloids and additional secondary compounds, frontiers in plant biochemistry. Prereq: BCH 607 or equivalent or consent of instructor. (Same as BCH/PPA 609.) .
Instructors: Ling Yuan David Hildebrand
Research/Teaching Publications
Books and Book Chapters
Pattanaik, S., Werkman, JR., Kong, Q. and Ling Yuan, 2009. Site-directed Mutagenesis and Saturation Mutagenesis for the Functional Study of Transcription Factors Involved in Plant Secondary Metabolite Biosynthesis. Metabolic Engineering of Plant Secondary Pathways. in Methods in Molecular Biology. (Book chapter; in press) 
Refereed Journal Articles
Bai, Y.H., Pattanaik, S., Patra, B., Werkman, J.R., Xie, C.H. and Yuan, L. 2011. Flavonoid-related basic helix-loop-helix regulator, NtAn1a and NtAn1b, of tobacco have originated from two ancestors and are functionally active. Planta. (in press) 
Jordan, D.B., Wagschal, K., Fan, ZM, Yuan, L., Braker, J.D. and Heng, C. 2011. Engineering lower inhibitor affinities in beta-D-xylosidase of Selenomonas ruminantium by site-directed mutagenesis of Trp145. Journal of Industrial Microbiology and Biotechnology. (in press) 
Fan, ZM and Ling Yuan. 2010. Production of Multifunctional Chimeric Enzymes in Plants: a promising Approach for Degrading Plant Cell Wall from Within. Plant Biotechnology Journal. 8:308-315 
Fan, ZM., Yuan, L., Jordan, DB., Wagschal, K., Heng, C., Braker, JD. 2010. Engineering Lower Inhibitor Affinities in Beta-D-xylosidase. Applied Microbiology and Biotechnology. 86(4):1099-1113 
Pattanaik, S., Kong, Q., Zaitlin, D., Werkman, JR., Xie, CH., Patra, B., and Ling Yuan, 2010. Isolation and functional characterization of a floral tissue-specific R2R3 MYB regulator from tobacco. Planta. 231(5):1061-1076 
Fan, Z.M., K. Wagschal, C.C. Lee, Q. Kong, K.A. Shen, I.B. Maiti, and L. Yuan. 2009. The construction and characterization of two xylan-degrading chimeric enzymes. Biotechnology and Bioengineering 102:684- 692. 
Fan, Z.M., J.H. Werkman, and L. Yuan. 2009. Engineering a multifunctional hemicellulase. Biotechnology Letters 31:751-757. 
Fan, Z.M., L. Yuan, D.B. Jordan, K. Wagschal, C. Heng, and J.D. Braker. 2009. Engineering lower inhibitor affinities in B-D-xylosidase. Applied Microbiology and Biotechnology. Published online: doi: 10.1007/s00253-009-2335-7. 
Fan, ZM, Ling Yuan, Chatterjee, R. 2009. Increased Hydrogen Production by Genetic Engineering of Escherichia coli. PLoS ONE 4(2): e4432 
Fan, ZM., Wagschal, K., Chen, W., Montross, MD., Lee, CC, and Ling Yuan, 2009. Multimeric Hemicellulases Facilitate Biomass Conversion. Applied and Environmental Microbiology. 75: 1754-1757 
Fan, ZM., Wagschal, K., Lee, C.C., Kong, Q., Shen, K.A., Maiti, I.B., and Ling Yuan, 2009. The Construction and Characterization of Two Xylan-degrading Chimeric Enzymes. Biotechnology and Bioengineering. 102(3): 684-692 
Fan, ZM., Werkman, J.R. and Ling Yuan, 2009. Engineering of a Multifunctional Hemicellulase. Biotechnology Letters. 31: 751-757 
Pattanaik, S., C.H. Xie, and L. Yuan. 2008. The interaction domains of the plant Myc-like bHLH transcription factors can regulate the transactivation strength. Planta 227:707-715. 
Authors in PSS Dept.: Ling Yuan Sitakanta Pattanaik
Pattanaik, S., C.H. Xie and L. Yuan 2008. The Interaction Domains of the Plant Myc-like bHLH Transcription Factors Can Regulate the Transcription Strength. Planta. 227(3): 707-715 
Pattanaik, S., C.H. Xie, and L. Yuan. 2008. Creation of master transcription factors to regulate plant metabolic pathways. Acta Horticulturae 756:109-118. 
Authors in PSS Dept.: Ling Yuan Sitakanta Pattanaik
Zeng, Q., F. Qiu, and L. Yuan. 2008. Production of artemisinin by genetically modified microbes. Biotechnology Letters 30:581-592. 
Zeng, QP, F. Qiu and L. Yuan 2008. Production of Artemisinin by Genetically-modified Microbes. Biotechnol Lett 30: 581-592 
Pattanaik, S., C.H. Xie and L. Yuan 2007. Creation of Master Transcription Factors to Regulate Plant Metabolic Pathways. Acta Hort. 756: 109-118 
Suttipanta, N., Pattanaik, S., Gunjun, S., Xie, CH, Littleton, J. and Yuan, L. 2007. Promoter analysis of the Catharanthus roseus geraniol 10-hydroxylase gene involved in terpenoid indole alkaloid biosynthesis. Biochim Biophys Acta, 1769:139-148 
Suttipanta, N., S. Pattanaik, S. Gunjan, J. Littleton, and L. Yuan. 2007. Promoter analysis of the Catharanthus roseus geranial 10-hydroxylase gene involved in terpenoid indole alkaloid biosynthesis. Biochemica et Biophysica Acta 1769:139-148. 
Authors in PSS Dept.: Ling Yuan Sitakanta Pattanaik
Chatterjee, R., and L. Yuan. 2006. Directed evolution of metabolic pathways. Trends in Biotechnology. 24(1): 28-38 
Pattanaik, S., C.H. Xie, Q. Kong, K.A. Shen, and L. Yuan 2006. Directed evolution of plant helix-loop-helix transcription factors for the improvement of transactivation properties. Biochim Biophys Acta, 1759: 308-318 
Yuan, L., I. Kurek, J. English and R. Keenan. 2005. Laboratory-directed protein evolution. Microbiology and Molecular Biology Reviews. 69(3): 373-392 
Zheng W, Wise ML, Wyrick A, Metz JG, Yuan L, Gerwick WH. 2002. Polyenoic fatty acid isomerase from the marine alga Ptilota filicina: protein characterization and functional expression of the cloned cDNA. Arch Biochem Biophys. 401(1):11-20. 
Facciotti, M., Burtain, P., and Ling Yuan 1999. Improved Stearate Phenotype in Transgenic Canola Expressing a Modified Acyl-acyl Carrier Protein Thioesterase. Nature Biotechnology. 17 (6): 593-597. 
Huang YS, Chaudhary S, Thurmond JM, Bobik EG Jr, Yuan L, Chan GM, Kirchner SJ, Mukerji P, Knutzon DS. 1999. Cloning of delta12- and delta6-desaturases from Mortierella alpina and recombinant production of gamma-linolenic acid in Saccharomyces cerevisiae. Lipids 34(7):649-59. 
Facciotti, M. T. andLing Yuan, 1998. Molecular Dissection of the Plant ACP-thioesterases. Fett/Lipid 100 (4-5): 167-172. 
Knauf, V.C and Ling Yuan, 1997. Modification of Plant Components. Current Opinion in Biotechnology. 8: 227-233. 
Ling Yuan, B. A. Nelson, and G. Caryl. 1996. The Catalytic Cysteine and Histidine in the Plant Acyl-ACP Thioesterases. Journal of Biological Chemistry 271: 3417-3419. 
Ling Yuan, T. A. Voelker and D. J. Hawkins 1995. Modification of the Substrate Specificity of an Acyl-ACP Thioesterase by Protein Engineering. Proceeding of National Academy of Science U.S.A. 92: 10639-10643.