标题：金双侠
基本概况
  姓名:金双侠[1] 
出生年月:1979.2 
性别:男 
民族:汉
硕博导:硕导
职称:副教授
研究方向:棉花组织培养,基因工程
个人简介
华中农业大学植物科学技术学院作物遗传育种专业博士，副教授，硕士生导师。1997-2001，华中农业大学农学系农学专业就读；2001-2006，华中农业大学植物科学技术学院攻读硕士、博士学位，获农学博士学位，指导教师均为张献龙教授。2007.10-2012.4在美国University of Central Florida，Henry Daniell 教授指导下进行博士后研究，研究方向为植物叶绿体转化。研究领域主要涉及棉花细胞、组织培养、棉花转基因等。目前从事棉花抗病，虫及逆境基因的克隆及高效表达载体（叶绿体转化表达体系）的开发及转基因研究。  
在攻读博士学位期间，建立了以棉花胚性愈伤组织为转化受体的高效遗传转化体系，研究结果发表在SCI刊源的杂志上，并利用该体系在国际上首次建立起棉花T－DNA插入突变体库，为棉花转基因育种提供了重要平台，也为棉花功能基因组研究奠定坚实的基础，同时发明了棉花转基因试管苗嫁接移栽技术，将棉花试管苗移栽成活率提高到90%以上，达到了国际先进水平；利用绿色荧光蛋白基因系统地研究了棉花遗传转化整个过程，并在国际上首次将超声波技术引入到棉花转基因技术体系中，建立了一种新的转基因体系，已经申报国家专利。
科研项目
国家自然科学基金 植物凝集素基因在棉花中的功能分析,主持,2012-2015 
新疆建设兵团博士资金项目 转基因抗病虫、抗逆聚合杂交棉新组合选育技术研究,主持,2007-2009 
国家自然科学基金 棉花外源基因叶绿体转化体系的建立及其应用(30771368),主持,2008-2010 
教育部博士点新教师基金 以gfp基因为报告基因监测转基因棉花花粉漂移(2007.0504087),主持,2008-2010 
国家自然科学基金重大国际合作项目 Development of strong pest-resistance cotton via transforming 
plastid and its application in breeding,主要参加者,2009-2011
国家自然科学基金 利用基因诱捕构建棉花功能基因组研究突变群体,主要参加,2005-2007 
发明专利及获奖情况获奖情况
获奖情况湖北省优秀博士学位论文（第10批）获奖者,2008年
湖北省优秀本科论文指导奖，2008年。指导的2007界本科生张碧瑶，其论文：新疆棉花高体细胞胚胎发生
能力基因型的筛选，获得湖北省优秀本科学位论文
“棉花分子育种技术体系建立与应用”获2008年湖北省技术发明二等奖
获第十、十一届湖北省自然科学优秀论文一等奖2次 申报两项发明专利：超声波辅助农杆菌转化棉花胚芽的方法，专利申请号：2007.10063650.0 
一种分离棉花叶绿体DNA的方法，专利申请号：2007.10052665.7 
发表的论文及著作
[1]Shuangxia Jin and Henry Daniell.Expression of γ-tocopherol methyltransferase inchloroplasts 
results in massive proliferation of the inner envelope membrane anddecreases susceptibility to salt 
and metal-induced oxidative stress by reducingreactive oxygen species.Plant Biotechnology 
Journal,Under review,2013. 
[2]Dheeraj Verma,Shuangxia Jin,Anderson Kanagaraj,Nameirakpam D.Singh1,Jaiyanth Daniel1,
Pappachan E Kolattukudy1,Michael Miller2 and Henry Daniell1* Expression of Fungal Cutinase and 
Swollenin in Tobacco Chloroplasts Reveals Novel Enzyme Functions and/or Substrates.PLOS 
ONE,Accepted,2013 (SCI,IF:4.0)
[3]Henry Daniell,KwangChul Kwon*,Dheeraj Verma*,ShuangxiaJin*.Export of proteins from chloroplasts 
induced by reactive oxygen speciesduring biotic and abiotic stress.Plant Cell,Revised,2012.(*These 
threeanthors contributed equally to this work). 
[4]Shuangxia Jin,Xianlong Zhang1,Henry Daniell.Pinellia ternata agglutininexpression in 
chloroplasts confers broad spectrum resistance against aphid,whitefly,lepidopteran insects, 
bacterial and viral pathogens.PlantBiotechnology Journal 2012,10(3):313-327(Cover Story,IF 5.44).
[5]Jin SX,liu GZ,Zhu HG,Yang XY and Zhang XL.Transformation of Upland Cotton(Gossypium hirsutum 
L.)with gfp Gene as a Visual Marker.Journal of IntegrativeAgriculture,2012,11(6):910-919,(SCI).
[6]Shuangxia Jin,Anderson Kanagaraj,Dheeraj Verma,Theo Lange,HenryDaniell.Release of Hormones 
from Conjugates:Chloroplast Expression ofß-glucosidase Results in Elevated Phytohormone Levels 
Associated withSignificant Increase in Biomass and Protection from Aphids or WhitefliesConferred by 
Sucrose Esters.Plant Physiol,2011,155,222-235.(SCI,IF:6.5).
[7]Seung-Bum Lee,Baichuan Li,Shuangxia Jin and Henry Daniell.Expression andcharacterization of　
antimicrobial peptides Retrocyclin-101and Protegrin-1 inchloroplasts to prevent sexual transmission 
of diseases.Plant BiotechnologyJournal.2011,9.100-115.(SCI,IF:5.44).
[8]Verma D,Kanagaraj A,Jin SX,Singh N,Kolattukudy P,Daniell H.Chloroplast-derived enzyme cocktails 
hydrolyse lignocellulosic biomass andrelease fermentable sugars.Plant Biotechnology 
Journal,2010,8:332-350.(SCI,IF:5.44).
[9]Jin SX,Zhang XL,Zhu HG,Zhou ZL,Nie YC.Detection of somaclonal variationof cotton (Gossypium 
hirsutum) through RAPD,SSR markers,cytogenetic and flowcytometer analysis.Plant Cell Reports, 
2008,27:1303–1316.(SCI).
[10]Shuangxia Jin,Xianlong Zhang,et al,Identification of a novel elitegenotype for in vitro culture 
and genetic transformation of cotton.BiologiaPlantarum,2006 50(4):519-524.(SCI).
[11]Shuangxia Jin,Xianlong Zhang（2006）Anefficient grafting system for transgenic plant recovery 
in cotton (Gossypiumhirsutum L.).Plant Cell,Tissue and Organ Culture,85(5):181-185.(SCI).
[12]Shuangxia Jin,Xianlong Zhang（2005）Factorsaffecting stable transformation and plant 
regeneration during transformingembryogenic callus of Upland cotton (Gossypium hirsutum L.) via 
Agrobacterium tumefaciens,Plant Cell,Tissue and Organ Culture,81(2):229-237.(SCI).
[13]Shuangxia Jin,Xianlong Zhang（2005）FunctionAnalysis of PromoterTrapping System after Inserting 
Cotton (Gossypium hirsutumL.)Genome.J GENET GENOMICS（Acta Genetica Sinca),32(12):1266-1274.
[14] Xianlong Zhang and Shuangxia Jin (2007) Transgenic Cotton:An Overview（invitedreview). 
Transgenic Plant Journal,2007,1(1),150-162. 
[15]Fang Ding,Shuangxia Jin,Ni Hong,Yun Zhong,Qing Cao,Ganjun Yi andGuoping Wang.2008. 
Vitriﬁcation–cryopreservation, an efﬁcient method foreliminating Candidatus Liberobacter 
asiaticus, the citrus Huanglongbingpathogen, from in vitro adult shoot tips.Plant Cell 
Reports,27(2),241-250(SCI).
[16]Sun Y Q,Zhang X L,Jin S X.Production and characterization of somatichybrids between upland 
cotton (Gossypium hirsutum) and wild cotton (G.klotzschianum Anderss) via electrofusion.Theor Appl 
Genet,2004,109:472-479(SCI).
[17]Sun Y Q,Zhang X L,Jin S X Somatic embryogenesis and plant regenerationin wild cotton (Gossypium 
klotzschianum Anderss).Plant Cell Tissure OrganCult,2003,75:247-253(SCI).
[18]Wu J H, Zhang X L, Jin S X．Factors affecting somatic embryogenesisand plant regeneration 
from a range of recalcitrant genetypes of Chinese cotton．In vitroCell Dev Biol-Plant, 2004, 40: 
371-375 (SCI).
[19]GUO X,JIN S,ZHANG X（2007）Agrobacterium-mediatedtransformation of Cry1C,Cry2A and Cry9C 
genes into Gossypium hirsutum and plantregeneration.Biologia Plantarum,51(2):242-248(SCI).
[20]Xiyan Yang,Xianlong Zhang,Shuangxia Jin,Lili Fu,Linggang WangProduction and characterization of 
asymmetric hybrids between upland cottonCoker201 (Gossypium hirsutum) and wild cotton (Gossypium 
klozschianumAnderss),Plant Cell Tissure Organ Cult,2007,89:225–235(SCI).
[21]Yang X,Guo X,Zhang X,Nie Y and Jin S.Plant regeneration from Gossypiumdavidsonii protoplasts 
via somatic embryogenesis.BIOLOGIA PLANTARUM,51(3):533-537,2007(SCI).
[22]Xianlong Zhang,Shuangxia Jin,Diqiu Liu,Zhongxu Lin,Fanchang Zeng,Longfu Zhu,Lili Tu,Xiaoping 
Guo.Cotton Biotechnology:Challenge the Future for Cotton Improvement,In: Biotechnology in 
CropImprovement,GP Rao (ed.),Studium Press,LLC,Houstan,Texas,USA.Pp.241-301
[23]Lili Tu,Xianlong Zhang,Diqiu Liu,Shuangxia Jin,Jinglin Cao,LongfuZhu,Fenglin Deng,Jiafu Tan, 
Cunbin Zhang,2007,Suitable internal controlgenes for qRT-PCR normalization in cotton fiber 
development and somaticembryogenesis, Chinese Science Bulletin 52:3110-3117 (SCI).
[24]周小凤，金双侠，李保成，余渝，宁新柱，王旭文.初步研究不同激素对海岛棉体细胞胚胎发生的影响.
中国农学通报2009,25(11):78-83.
[25]朱华国, 涂礼莉, 金双侠, 徐理, 谭家福, 邓锋林, 张献龙.棉花细胞初始脱分化的基因差异表达分析.科学
通报，2008，53 （20），2483-492(SCI).
[26]曹景林 张献龙 金双侠 杨细燕 朱华国 付莉莉.棉花高效体细胞胚发生及同步控制培养体系研究.作物学
报,2008, 34(2): 224−231
[27]谢德意，金双侠，郭小平，张献龙．长江和黄河流域棉区棉花品种体细胞胚胎发生和植株再生能力研究.
作物学报，2007，33（3）：394－400
[28] 谢德意，金双侠，郭小平，张献龙．棉花ipt基因遗传转化效率及优化成苗技术研究.作物学报，2007,
33（5).
[29]金双侠, 韩杰,刘小云,刘冠泽,王一娴,唐文鑫,张献龙.SDS-蛋白酶法分离棉花cpDNA及psbA基因启动子、
终止子克隆.分子植物育种,2007,5,683-689.
[30]周小凤，张碧瑶，刘冠泽，高巍，余渝，邓福军，李保成，孔宪辉，张献龙, 金双侠*.新疆棉花高体细胞
胚胎发生能力基因型的筛选，分子植物育种, 2007,6, 1-8(通讯作者).
[31]张献龙，孙玉强，吴家和，金双侠，聂以春，郭小平，2004，棉花细胞工程及新种质创造，棉花学报，
16（6）：368-373.