Task: Class Definition Understanding (ontology: Food_Agriculture/Crop_Ontology)

Which of the following definitions best describes 'Total anthocyanins Cy-3-gluc method'?

A. Measure total anthocyanins of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag. Total anthocyanins were measured using the pH differential method as described by Lee et al. (2005) as the change in absorbance at 2 different pH values of 1.0 and 4.5.
B. Observe the diameter of the non-terminal flowers at fully open. Remontant plants should be observed at first flower flush.
C. A 0.5 g aliquot of fruit puree is combined with 24.5 mL deionized water and a 0.5 mL aliquot is placed on refractometer. Percent titratable acid is expressed as equivalents citric acid.
D. Record the Julian date when first fruit ripens at a specific location.

Answer: Measure total anthocyanins of room temperature, homogenized fruit pulp samples by combining fruit and any juice lost during thawing in a zip top bag. Total anthocyanins were measured using the pH differential method as described by Lee et al. (2005) as the change in absorbance at 2 different pH values of 1.0 and 4.5.