antibody response it's literally this test and they'll get a stronger readout at the end of this test if you've had two doses or three doses or whatever um but the way that you actually detect it is interesting it's so weird there are antibodies that will stick to literally freaking everything and so like let's say this test is using um your serum and so your serum has human antibodies in it well there is an antibody that sticks to human antibodies and so it can be like originally you can make these things with like by like vaccinating a goat with human antibodies and then that goat will make its own goat antibodies that stick to human antibodies um and you can do that with everything but these days now you can just make them synthetically so like it's known how to make an antibody that will stick to human antibodies and for instance i can make it in plants like you don't even need a goat you can just make it in plants now or you can make it in whatever um but that those antibodies have had a um a pro um god how do i say this simply it's called horseradish peroxidase because it's a it's a it's an enzyme that was discovered in horseradish that will cause a chemical reaction that causes a color change and they have stuck that horseradish peroxidase onto an antibody um so if you um if i mix that antibody that sticks to human antibodies with this horseradish peroxidase thing stuck to it it will make these little wells turn blue if they contain human antibodies or whatever so so what it makes it turn blue if the antibodies are there and not if it's not there finally i added a solution of acid that stops the color change from happening and then i took it to a machine that reads exactly how much color has been made in my case i tested four different ideas for vaccines that i've been trying to make against norovirus two of them worked pretty well and two did not work at all so this was my first attempt at making this norovirus vaccine thing and i tried what i tried doing is i tried um i tried giving the mice um remember that like ball thing or whatever like if you think about the part if you think about like the part of that like shell or whatever if you just think about one part of it there's like the tip that changes a lot and there's sort of like middle segments and lower segments i tried vaccinating only with the tip only with like the top segment only with the like lower segment only with the base segment and two of those worked kind of well and two of them didn't work at all it seems like there's um some part at the base that works really well and some part in the center but the other ones the other ones didn't work super well and so the next idea that i'm trying right now is like okay i'm gonna do the whole thing and just like take out a few little pieces rather than only giving the mice a few pieces in the first place so next i need to figure out why those two didn't work and i'm going to see if i can come up with any new ideas to try to see if i can make this potential vaccine even better though in reality there are dozens of other tests that also need to be done to figure out whether a vaccine worked well or not but this is
START_SELFPROMO_TOKEN antibody response it's literally this test and they'll get a stronger readout at the end of this test if you've had two doses or three doses or whatever um but the way that you actually detect it is interesting it's so weird there are antibodies that will stick to literally freaking everything and so like let's say this test is using um your serum and so your serum has human antibodies in it well there is an antibody that sticks to human antibodies and so it can be like originally you can make these things with like by like vaccinating a goat with human antibodies and then that goat will make its own goat antibodies that stick to human antibodies um and you can do that with everything but these days now you can just make them synthetically so like it's known how to make an antibody that will stick to human antibodies and for instance i can make it in plants like you don't even need a goat you can just make it in plants now or you can make it in whatever um but that those antibodies have had a um a pro um god how do i say this simply it's called horseradish peroxidase because it's a it's a it's an enzyme that was discovered in horseradish that will cause a chemical reaction that causes a color change and they have stuck that horseradish peroxidase onto an antibody um so if you um if i mix that antibody that sticks to human antibodies with this horseradish peroxidase thing stuck to it it will make these little wells turn blue if they contain human antibodies or whatever so so what it makes it turn blue if the antibodies are there and not if it's not there finally i added a solution of acid that stops the color change from happening and then i took it to a machine that reads exactly how much color has been made in my case i tested four different ideas for vaccines that i've been trying to make against norovirus two of them worked pretty well and two did not work at all so this was my first attempt at making this norovirus vaccine thing and i tried what i tried doing is i tried um i tried giving the mice um remember that like ball thing or whatever like if you think about the part if you think about like the part of that like shell or whatever if you just think about one part of it there's like the tip that changes a lot and there's sort of like middle segments and lower segments i tried vaccinating only with the tip only with like the top segment only with the like lower segment only with the base segment and two of those worked kind of well and two of them didn't work at all it seems like there's um some part at the base that works really well and some part in the center but the other ones the other ones didn't work super well and so the next idea that i'm trying right now is like okay i'm gonna do the whole thing and just like take out a few little pieces rather than only giving the mice a few pieces in the first place so next i need to figure out why those two didn't work and i'm going to see if i can come up with any new ideas to try to see if i can make this potential vaccine even better though in reality there are dozens of other tests that also need to be done to figure out whether a vaccine worked well or not but this is END_SELFPROMO_TOKEN