VictorJuiz/thesis huggingface dataset:
Flexible Distributed Business Process Management

Many large business processes are inherently distributed, spanning multiple organizations, administrative domains, and geographic locations. To support such applications, this thesis develops a flexible and distributed platform to develop, execute, and monitor business processes. The solutions utilize a distributed content-based publish/subscribe overlay that is extended with support for mobile clients and client interest churn. Over this layer, a distributed execution engine uses events to coordinate the execution of the process, and dynamically redeploys activities in the process in order to minimize a user-specified cost function and preserve service level agreements (SLAs). Finally, a management layer allows users to find and automatically compose services available across a distributed set of service registries, and monitor processes for SLA violations. Evaluations show that the distributed execution engine can scale better than alternate architectures, exhibiting over 60% improvements in execution time in one experiment. As well the system can dynamically redeploy processes to reflect changing workload conditions and SLAs, saving up to 90% of the process messaging overhead of a static deployment.

Keywords: publish/subscribe
business process
Advisor: Hans-Arno, Jacobsen
Regulation of Metalloproteinase-dependent Ectodomain Shedding in Cytokine Biology and Inflammation

In 1962, Gross and Lapiere described collagenolytic activity in the degradation of tadpole tails during amphibian metamorphosis. This activity was later attributed to a collagenase enzyme belonging to the matrix metalloproteinase family. Over the past 49 years, steady growth in the field of metalloproteinase biology has uncovered that degradation of extracellular matrix components represents only a fraction of the functions performed by these enzymes. The regulatory roles of these enzymes in numerous aspects of mammalian biology remains poorly understood. This thesis investigates the metalloproteinase ADAM17 and its natural inhibitor TIMP3 in acute and chronic inflammation. My work describes the generation of new murine experimental systems of compartmentalized ADAM17 or TIMP3 deficiency and their applications in acute liver inflammation (i.e. fulminant hepatitis and T-cell mediated autoimmune hepatitis) and atopic dermatitis. Loss of Timp3 protected mice against fulminant hepatic failure caused by activation of the death receptor Fas. We determined that TIMP3 simultaneously promotes pro-apoptotic signaling through TNFR1 while suppressing anti-apoptotic EGFR activation in the liver. Mechanistically, we identified that ADAM17 is critical in shedding TNFR1 and EGFR ligands (e.g. Amphiregulin, HB-EGF, TGF) and extended this finding to clinically relevant drug-induced hepatitis. Adult TIMP3 deficient mice also exhibited spontaneous accumulation of CD4+ T cells in the liver. Consequently, polyclonal T cell activation with the lectin Concanavalin A (con A) in a model of autoimmune hepatitis resulted in accelerated liver injury. We identified that this immunopathology relied on TNF bioavailability as mice lacking both Timp3 and Tnf were resistant to con A. Using bone marrow chimeras we established that non-hematopoietic tissues were the physiologically relevant source of TIMP3 in vivo, thereby highlighting an immunosuppressive role for this stromal metalloproteinase inhibitor in cellular immunity. Finally, we investigated epithelial:immune crosstalk in the epidermis by generating tissue-specific ADAM17 deficiency in basal keratinocytes. These mice developed spontaneous inflammatory skin disease that was physiologically consistent with atopic dermatitis. Focused investigation of keratinocyte-specific signaling deregulated by ADAM17 deficiency revealed its requirement for tonic Notch activation, which in turn antagonized transcriptional activity of AP-1 transcription factors on the promoters of epithelial cytokines TSLP and G-CSF. In summary, these works identify cellular mechanisms governing cytokine-mediated communication between epithelial and immune cells to modulate inflammation. The findings that TIMP3 and ADAM17 act as regulators of key inflammatory, proliferative and developmental pathways provide impetus to expand our understanding of this important family of enzymes in mammalian signal transduction.

Keywords: Immunology
Genetics
Metalloproteinase
Cytokine
Inflammation
Signaling
Advisor: Khokha, Rama
The Importance of Thermal Habitat Quality for Pumpkinseed (Centrarchidae: Lepomis gibbosus) in Small and Constructed Coastal Embayments Along the Northwest Shoreline of Lake Ontario

Along the Toronto shoreline, small coastal embayments (0.4 – 32 ha) are being constructed or modified to restore warmwater fish habitat. I describe how Lake Ontario (hereafter the Lake) alters the thermal regime of these small coastal embayments, how the altered thermal regimes affect growth and survival of age-0 warmwater fishes, and how the thermal habitat quality for such fishes can be improved by altering embayment design. During the warming period of the ice-free season, embayments warm faster than the Lake and so are cooled by exchanges with the Lake. Later in the year Lake exchange warms the rapidly cooling embayments, but the net effect of Lake-embayment exchange is cooling. The degree of cooling in Toronto’s small coastal embayments varies; many have temperatures near that of the Lake, and a few warm as much as local ponds. Age-0 pumpkinseed (Lepomis gibbosus) can fail to reach sufficient size to survive the winter in cooler embayments because their spawning is delayed and their growth is slowed. Most embayments along the Toronto shoreline are too cold to produce age-0 fish that can survive the winter, but all embayments are occupied by age >1 pumpkinseed, suggesting movement from warmer to cooler embayments. Using otolith microchemistry to identify natal embayments of fish, I confirm that age-0 and age-1 pumpkinseed, as well as age-0 largemouth bass (Micropterus salmoides) and age-0 yellow perch (Perca flavescens), exist in metapopulations. Embayment bathymetry is a poor predictor of temperature because almost all embayment flushing rates are very fast, usually 1-2 days. Warmer embayments are located in protected areas of Lake Ontario and receive waters that have already heated substantially. Cold embayments are located along the exposed shoreline of Lake Ontario. To protect embayments from cold lake waters, the cross-sectional area of embayment channels need to be reduced to 1-10% of their current size.

Keywords: Aquatic
Fisheries
Coastal Embayments
Lake Ontario
Toronto
Great Lakes
Advisor: Collins, Nicholas
Adrenalectomy and Glucocorticoid Effects on the Rat Hepatic Aryl Hydrocarbon Receptor Pathway and the Response to Aromatic Hydrocarbons

The aryl hydrocarbon receptor (AHR) is a ligand-activated transcription factor that mediates the effects of aromatic hydrocarbons, such as 2,3,7,8-tetrachlorodibenzo-p-dioxin and 3-methylcholanthrene (MC); the prototypical response is induction of drug-metabolizing enzymes. Factors that regulate AHR levels in vivo are poorly understood. It is also not clear how AHR levels affect MC responsiveness. I hypothesize that glucocorticoids enhance hepatic AHR based on previous findings of decreased hepatic AHR protein in hypophysectomised rats and increased AHR levels following glucocorticoid treatment in rodent hepatoma cells. To study this, adrenalectomized (ADX) or SHAM-ADX rats were treated with dexamethasone or vehicle. AHR protein was decreased by 50–60% at 4 days after ADX, but was not altered by dexamethasone. Dexamethasone induced hepatic AHR nuclear translocator (ARNT) mRNA by up to 9-fold, with no corresponding change in ARNT protein. AHR target gene expression was measured in MC-treated ADX rats to assess MC responsiveness given the decrease in AHR protein following ADX. MC-induced hepatic CYP1B1 mRNA was reduced by 50% in ADX rats relative to SHAM. AHR mRNA was increased 4-fold, 6 h after MC in SHAM rats, but no induction was observed in ADX rats. MC-induced 7-ethoxyresorufin O-deethylation activity in ADX rats was 35% of the activity in the MC-treated SHAM group at 6 h. To assess the capacity for hepatic P450-mediated metabolism, NADPH-cytochrome P450 oxidoreductase (POR) was measured. POR activity was decreased by 50-65% following ADX. DEX induced hepatic POR mRNA by up to 7-fold, 6 h after treatment in SHAM, ADX and intact rats. Putative glucocorticoid responsive elements in the rat Por gene were identified, but recruitment of the glucocorticoid receptor to these elements was not detected using chromatin immunoprecipitation. In rat H-4-II-E hepatoma cells, dexamethasone induced POR, but not ARNT, mRNA. I have shown that ADX decreases hepatic AHR protein and subsequently, MC responsiveness is suppressed for some AHR-mediated responses. Decreased POR activity following ADX might contribute to a decreased capacity for P450-dependent metabolism. The novel findings with respect to glucocorticoid regulation of ARNT and POR demonstrate the complexity of AHR-glucocorticoid cross-talk and the need for further study.

Advisor: Riddick, David S.
Interorganizational Partnerships, Leadership, Structures, and Processes: A Case Study of the Arab Bureau of Education for the Gulf States (ABEGS)

The purpose of this study was to identify factors that influence leadership and its distribution in the Arab Bureau of Education for the Gulf States (ABEGS) Saudi Arabia. The research was undertaken in the ABEGS as a case study and its two initiatives, the Gulf Arab States Educational Research Centre (GASERC) Kuwait, and the Arab Educational Training Centre for Gulf States (AETCGS) Qatar respectively. The findings of this study reveal that the leadership in this interorganizational environment is distributed within the four levels of organizational structures. The visionary leadership comes from the inner most core political level that has its influence and direction at the strategic, managerial, and operational levels of the organization. . Based on extensive research of Leithwood and his colleagues, this study will integrate four leadership functions of setting directions, structuring the organization, developing capacity, and managing the (partnership) program into the analysis of interorganizational partnerships. The study also examined ‘securing accountability’ as another important leadership function in the partnership environment. I learnt that multiple factors influence leadership and enable different individuals and groups to perform these functions at the strategic, managerial and operational levels of the interorganizational structures. However, the major influence on leadership that weaves through the strategic level to the grassroots levels is the consultative process embedded in the organizational structures of the Arab Bureau. Some other prominent factors that influence leadership found in the study are positional power in the hierarchy, experience and knowledge, and dedication and commitment. Researchers like Benson, Mawhiney, Kickert, Proven, and Rodríguez, explored interorganizational partnerships and view the structures of leadership either vertically centralized or horizontally distributive. I argue that interorganizational leadership works horizontally at each level of the partnership i.e. strategic, managerial, and operational and vertically between these levels in the ABEGS partnership as shown in the ‘Circular Model of Interorganizational Leadership’ of this study. The leaders at various levels in the Arab Bureau from the member states function as equals. However, firm vertical hierarchy exists between various levels in the structures of the ABEGS. It is therefore established that horizontal and vertical leadership work simultaneously in interorganizational partnership environments as found in the case study of the Arab Bureau.

Keywords: Education
Leadership
Interorganizational Partnership
Partnership
Educational Administration
Arab Bureau
ABEGS
Circular Model of Interorganizational Leadership
Leadership Model
Circular Model
Advisor: Anderson, Stephen
Molecular Basis of Abnormal Conduction in Mice Over-expressing Endothelin-1

Binary transgenic (BT) mice with doxycycline (DOX)-suppressible cardiac-specific over-expression of endothelin 1 (ET 1) exhibit progressive heart failure, QRS prolongation, and death following DOX withdrawal. However, the molecular basis and reversibility of the electrophysiological abnormalities in this model were not known. Here we assess the mechanisms underlying ET 1 mediated electrical remodelling, and its role in heart failure. Prior attempts to prevent this model of ET-1 induced cardiomyopathy with ET receptor antagonism were not beneficial. We now propose to evaluate the effectiveness of blocking the synthesis of ET-1 with CGS 26303, a dual inhibitor of endothelin converting enzyme (ECE) and neutral endopeptidase. BT vs. littermate control mice were withdrawn from DOX and serially studied with ultrasound biomicroscopy, octapolar catheters, multi-electrode epicardial mapping, histopathology, Western blot, immunohistochemistry and qRT-PCR. Prolonged ventricular activation and depressed rate of ventricular activation were detected as early as 4 wks after transgene activation, when structure and function of the heart remained unaffected. By 8 wks of ET-1 over-expression, biventricular systolic and diastolic dysfunction, myocardial fibrosis, cardiomyocyte hypertrophy, prolonged ventricular activation and repolarization, depressed rate of ventricular activation, and abnormal atrioventricular nodal function were observed. Within 4 wks of ET-1 induction, reduction were observed in connexin-43 mRNA, protein, and phosphorylation, Nav1.5 mRNA and protein, Na+ conductance, K+ channel interacting protein-2 mRNA and Kv4.2 mRNA. Chromatin immunoprecipitation revealed that nuclear factor κB preferentially binds to Cx43 and Nav1.5 promoters. Importantly, the associated electrophysiological abnormalities at this time point were reversible upon suppression of ET 1 over-expression and completely prevented the development of structural and functional remodelling. Treatment with CGS-26303 (5 mg/kg/day) failed to improve survival, or hemodynamic and contractile decline. ET-1-mediated ventricular conduction delays correlates with gap junction and ion channel remodelling, and precedes heart failure. The sequence and reversibility of this phenotype suggest that a primary abnormality in electrical remodelling may contribute to the pathogenesis of heart failure. CGS 26303 failed to prevent this cardiomyopathic phenotype. These data suggest that chronically high levels of bigET-1, as seen in heart failure, may induce increased ECE activity and/or non-ECE ET-1 synthesis, thus circumventing the efficacy of ECE blockade in this model.

Keywords: heart failure
endothelin-1
cardiac electrophysiology
ion channels
Advisor: Husain, Mansoor
Stewart, Duncan John
"The Word is Not all Rainbows and Butterflies": Facilitating Physical Activity and Quality of Life among Children Living with Cystic Fibrosis and Congenital Heart Disease-toward a Conceptual Framework and Parent-mediated Behavioural Counselling Program

Although medical advances have improved prognosis for children with cystic fibrosis (CF) and congenital heart disease (CHD), these youth experience poor psycho - social health. Embedded within the Medical Research Council’s framework, the purpose of this Dissertation was to a) examine how CF and CHD children experience physical activity, b) explore parents’ perceptions toward their child’s activity, c) develop a theory of physical activity in childhood chronic diseases, d) develop a physical activity counselling program for youth with CF and their parents, and e) evaluate the program impact on quality of life and physical activity. Study One explored how 14 CF youth experience physical activity. Active and inactive youth were characterized by different experiences, such as a sense of hope or despair. Given the burden of treatment and the fatal nature of the disease, youth negotiated temporal barriers to activity. Study Two explored perceptions toward activity among 29 CF and CHD parents. Parents discussed the benefits and barriers associated with physical activity for both child and self, and underscored the importance of role modeling. By adopting a Grounded Theory approach, the theoretical constructs from Study One and Two were crystallized to develop a theory of physical activity in youth with CF and CHD. This theory was used to develop “CF Chatters:” A Six Week Physical Activity Counselling Program for Youth with CF and their Parents, and the intervention employed behavioural self regulation ii skills. In Study Three, four case families in the CF clinic at the Hospital for Sick Children participated in CF Chatters. Improvements were noted in quality of life and physical activity, and participants described the program as convenient and relevant to their activity concerns. CF Chatters afforded therapeutic benefits to participants. By employing an eclectic qualitative approach, this Doctoral Program has made theoretical and practical contributions toward our understanding of how physical activity is experienced among children living with CF/CHD. The findings support the use of behavioural counselling as an effective and feasible modality for enhancing quality of life and physical activity. This Dissertation calls on clinicians to attend to the activity needs of chronically ill Canadian youth.

Keywords: Cystic Fibrosis
Congenital heart disease
Psychology
Physical activity
qualitative research methods
Advisor: Faulkner, Guy
A Calibration Free Estimation of the Point of Gaze and Objective Measurement of Ocular Alignment in Adults and Infants

Two novel personal calibration procedures that do not require active user participation are presented. These procedures, in conjunction with a state-of-the-art remote eye-gaze tracking (REGT) technology, allow estimation of the angle between the optical and visual axes (angle kappa) automatically without explicit/active user involvement. The first algorithm for the binocular estimation of angle kappa (BEAK) is based on the assumption that at each time instant both eyes look at the same point on a surface with a known geometry (e.g., a computer monitor). The sensitivity of the BEAK procedure to the geometry of the observation surface and to the noise in the estimates of the optical axis is studied both analytically and in computer simulations. Experimental results with 4 adult subjects suggest that with the current REGT technology angle kappa can be estimated with an RMS error of 0.5°. The second personal calibration algorithm (‘calibrate and validate’, CaVa) adopts a probabilistic approach to the estimation of angle kappa in infants. Even though the presentation of visual stimuli at known positions is part of the procedure, the CaVa algorithm does not require/assume continuous fixation on the presented targets. If an infant attends to roughly half of the presented targets, angle kappa can be estimated accurately and with high confidence. In experiments with five babies, the average difference between repeated measurements of angle kappa was 0.04 ± 0.31°. The second part of the thesis describes two methods for automated measurement of eye misalignment in adults and infants. These methods are based on the user-calibration-free (UCF) technology presented in the first part of the thesis. The first method is based on the clinical Hirschberg test. It is shown that the UCF-REGT technology can improve significantly the accuracy of the Hirschberg test by enabling the estimation of subject-specific parameters (the Hirschberg ratio and angle kappa) in infants. The maximum error in the estimation of the horizontal and vertical components of eye misalignment in five orthotropic infants was shown to be less than 1°, which is significantly better than the accuracy of a standard clinical Hirschberg test. Finally, a novel Eye-Tracker Based Test (ETBT) for the estimation of the maximum (manifest + latent) angle of deviation is presented. ETBT is based on the UCF-REGT system. ETBT allows free head movements and does not require continuous fixation on specific targets. Experiments with 22 adult subjects demonstrated a good agreement of 0.7 ± 1.7° between ETBT and the gold-standard clinical procedure—the altenate prism and cover test. A pilot study with 5 orthotropic infants and one infant with strabismus demonstrated that the ETBT can be used in infants.

Keywords: calibration-free
eye-tracking
strabismus
angle kappa
optical axis
visual axis
Advisor: Eizenman, Moshe
Cultural Uses of Magic in Fifteenth-century England

This dissertation examines the ways that books can show the place of magic in fifteenth-century English society. Specifically, I am interested in what was important about magic to people and how magic was used by people in the creation of their identities, both as individuals and within the community. As I explore these issues, I aim to demonstrate that magic freely co-mingled with non-magical texts in manuscripts. Furthermore, this mixing of magical and non-magical texts is a vital part of understanding magic’s role in the shaping of people’s identities, both public and private. Chapter one presents the results of a preliminary survey of magic in fifteenth-century English manuscripts. I clarify how I delineate between texts – magical and non-magical and between genres of magic. This chapter also uses a series of case studies to look at some of the issues of ownership that are dealt with in more detail in the later chapters of this thesis. Chapters two, three, and four look at individual manuscripts in depth. In Chapter two, I examine how a lower gentry household used their notebook to establish their place within a strata of the gentry that was increasingly interested in medical and scientific texts in the fifteenth century. Chapter three looks at the private notebook of an anonymous scribe and how its owner combines the ordinary and transgressive qualities of magic to create an identity for himself that is based on a quasi-clerical masculinity and the ludic qualities of magic. Chapter four concerns Robert Taylor’s medical notebook, which he may have used as a part-time medical practitioner, and the insight it gives into the everyday concerns of medieval people. Chapter five is an examination of the book of an early fifteenth-century Cistercian monk named Richard Dove. Dove’s notebook contains a copy of the Ars notoria, the only manuscript containing ritual magic that I study in this dissertation. I argue that Dove, unlike other monastic users of the Ars notoria, does not use the text for its spiritual benefits, but its material benefits as part of his desire to participate in a broader intellectual culture outside the monastery.

Keywords: magic
fifteenth-century England
manuscripts
book history
charms
natural magic
ritual magic
identity
Advisor: Gillespie, Alexandra
Goering, Joseph