VictorJuiz/thesis huggingface dataset:
A Spin-torque Transfer MRAM in 90nm CMOS

This thesis presents the design and implementation of a high-speed read-access STT MRAM. The proposed design includes a 2T1MTJ cell topology, along with two different read schemes: current-based and voltage-based. Compared to the conventional read scheme with 1T1MTJ cells, the proposed design is capable of reducing the loading on the read circuit to minimize the read access time. A complete STT MRAM test chip including the proposed and the conventional schemes was fabricated in 90nm CMOS technology. The 16kb test chip's measurement results confirm a read access time of 6ns and a write access time of 10ns. The read time is 25% faster than other works of similar array size published thus far, while the write time is able to match the fastest result.

Keywords: Spintronics
Solid-state memory
Non-volatile
MRAM
STT
MTJ
High-speed read-access
2T1MTJ cell
Advisor: Sheikholeslami, Ali
Characterization of Activated Carbon for Taste and Odour Control

Iodine number, BET surface area, taste and odour compound isotherms, and trace capacity number tests were used to rank five different granular activated carbons according to thermodynamic adsorption performance. These tests were compared to expected activated carbon service life and loading results of rapid small-scale column tests (RSSCTs) run with water from two lake sources spiked with geosmin and 2-methylisoborneol (MIB). Trace capacity number, used to specifically identify high adsorption energy sites on activated carbon, was hypothesized to be correlated to geosmin/MIB breakthrough and loading performance of different activated carbons. This study found no such clear correlation. However, when only bituminous coal activated carbons were considered, correlations to MIB breakthrough were strengthened. Natural organic matter (NOM) adversely affected adsorption, resulting in decreased RSSCT throughput to breakthrough in surface water with higher total organic carbon (TOC). Methods for improving characterization tests and RSSCTs when NOM is present are discussed.

Keywords: activated carbon
geosmin
MIB
water treatment
Advisor: Hofmann, Ronald
The Law Applicable to International Trade Transactions with Brazilian Parties: A Comparative Study of the Brazilian Law, the CISG, and the American Law About Contract Formation

Despite Brazil’s importance in the world economy and its increasing participation in foreign trade, there is considerable legal uncertainty regarding the law applicable to international commercial contracts involving Brazilian parties because Brazilian judicial courts do not respect parties’ freedom to choose the governing law, thus this determination is only made by a judge, according to Private International Law rules of the forum. Applying these rules, this study demonstrates that there are at least three potential legal regimes: the Brazilian law, the United Nations Convention on Contracts for the International Sale of Goods, and a foreign domestic sales law. Making use of the American law as the foreign law, a comparative analysis of these three legal regimes regarding contract formation demonstrates that their approaches are very distinct, and this confirms the legal uncertainty. In order to reduce this problem, three different strategies are proposed to the Brazilian government.

Keywords: LAW
INTERNATIONAL
TRADE
COMMERCIAL
SALE
GOODS
CONTRACT
FORMATION
BRAZIL
CISG
UNITED STATES
BRAZILIAN LAW
AMERICAN LAW
LAW APPLICABLE
PRINCIPLE OF PARTY AUTONOMY
PRIVATE INTERNATIONAL LAW
COMPARATIVE LAW
Advisor: Valcke, Catherine
Role of Vascular Endothelial Growth Factor-A in Diabetic Kidney Disease

Vascular endothelial growth factor-A (VEGF) is required for endothelial cell differentiation and survival. To investigate the renoprotective properties of VEGF in diabetes an inducible Cre-loxP gene targeting system was used to excise VEGF from podocytes of adult mice (VEGFKO). Diabetes was induced by streptozotocin (STZ) at 2.5 weeks of age and VEGFKO was induced by doxycycline (dox) at 3-4 weeks of age. Blood and urine were collected weekly to monitor for hyperglycaemia and proteinuria, respectively. Mice were dissected 8 weeks after diabetes induction or earlier if morbidly ill; twenty percent of the mice in the DM+VEGFKO group died before the surrogate endpoint. Glomerular VEGF mRNA expression was decreased in VEGFKO mice compared to controls. However, DM+VEGFKO mice had significantly greater proteinuria, degrees of glomerular sclerosis, and glomerular cell apoptosis. These results confirm that VEGF is normally upregulated in diabetes but reducing VEGF expression in diabetes causes severe kidney injury.

Keywords: VEGF
diabetes
VEGF-A
kidney
glomerulus
Vascular Endothelial Growth Factor A
Cre
mouse
mice
nephropathy
Advisor: Quaggin, Susan E.
In Vitro Assessment of Osteoblast Behavior in Craniosynostosis

Introduction: The objective of this study is to investigate the role of osteoblasts in the pathophysiology of premature suture fusion in infants. Methods: Bone and periosteal tissue from fused and patent cranial sutures and adjacent bone were harvested from infants undergoing surgery for craniosynostosis and used to develop primary osteoblast cell cultures. Dural tissue was obtained from neurosurgical procedures in order to generate an osteoblast-dural co-culture. Osteoblast proliferation, differentiation, mineralization, protein expression (Noggin, BMP3 and Runx2) and response to exogenous FGF2 stimulation were assessed. Results: Cell cultures demonstrated significant (p<0.05) regional variations in osteoblast proliferation, differentiation markers and in vitro bone nodule formation. The expression of anti-osteogenic molecules (Noggin and BMP3) was decreased in osteoblasts from fused suture regions. Conclusion: The creation of a pro-osteogenic environment through the decreased expression of anti-osteogenic signalling molecules and increased expression of osteogenic factors may be responsible for premature suture fusion in infants.

Keywords: craniosynostosis
cell culture
osteoblast behavior
anti-osteogenic signalling
dura mater
pro-osteogenic signalling
Advisor: Forrest, Christopher
A Comparative Evaluation of Plastic Property Test Methods for Self-consolidating Concrete and Their Relationships with Hardened Properties

Self-consolidating concrete (SCC) is a special type of concrete that flows under its own weight and spreads readily into place while remaining stable. Although SCC technology has been rapidly progressing over the last 20 years and continues to develop, the relationships between the fresh, hardened and durability properties of SCC are not well documented. The focus of this investigation is twofold. Firstly, the use of SCC necessitates reliable and accurate characterization of material properties. A variety of laboratory test methods are used to evaluate SCC’s plastic properties. Recognizing that various test methods evaluate the same plastic properties, there is a need to critically investigate the adequacy and sensitivity of each test. Secondly, outcomes from this project are expected to advance the fundamental understanding of the interplay between the fresh properties of SCC and their implications on hardened properties and durability performance.

Keywords: self-consolidating concrete
SCC
plastic properties
filling ability
passing ability
segregation
stability
durability
test method
Advisor: Panesar, Daman
Mechanism of MicroRNA miR-520g Pathogenesis in CNS-PNET

We recently discovered a high-level amplicon spanning the chr19q13.41 microRNA cluster in CNS Primitive Neuroectodermal Tumour, which results in striking upregulation of miR-520g. Constitutive over-expression of miR-520g in untransformed human neural stem cells enhanced cell growth, restricted differentiation down the neuronal lineage, and promoted expression of neural stem/progenitor cell markers. We thus hypothesize that ectopic miR-520g expression promotes tumourigenesis in part by inhibiting cellular differentiation. Consistent with this proposition, miR-520g is silenced upon embryonic stem cell differentiation and its expression is absent from most adult tissues. Moreover, expression analysis of miR-520g overexpressing cells revealed significant dysregulation of developmental signalling pathways. Further efforts focused on elucidating mechanisms of miR-520g function led to the identification of a cell cycle inhibitor, p21, as an important candidate target. These findings collectively suggest that miR-520g may modulate differentiation by regulating developmental signalling pathways and cell cycle exit of neural stem/progenitor cells.

Keywords: microRNA
brain tumour
cell cycle
development
Advisor: Huang, Annie
Task Re-allocation Methodologies for Teams of Autonomous Agents in Dynamic Environments

Two on-line task re-allocation methodologies capable of re-allocating agents to tasks on-line for minimum task completion time in dynamic environments are presented herein. The first methodology, the Dynamic Nearest Neighbour (DNN) Policy, is proposed for the operation of a fleet of vehicles in a city-like application of the dial-a-ride problem. The second methodology, the Dynamic Re-Pairing Methodology (DRPM) is proposed for the interception of a group of mobile targets by a dynamic team of robotic pursuers, where the targets are assumed to be highly maneuverable with a priori unknown, but real-time trackable, motion trajectories. Extensive simulations and experiments have verified the DNN policy to be tangibly superior to the first-come-first-served and nearest neighbour policies in minimizing customer mean system time, and the DRPM to be tangibly efficient in the optimal dynamic re-pairing of multiple mobile pursuers to multiple mobile targets for minimum total interception time.

Keywords: on-line task allocation
autonomous agents
Advisor: Benhabib, Beno
To MMR or not MMR: Medical Discourses Surrounding Parental Decision-making for Pediatric Immunization

Coverage for the combined measles, mumps, and rubella vaccine (MMR) has been low since the publication of Wakefield’s 1998 study associating MMR with the onset of autism. As a part of a larger project on risk communication, this study examined the medical discourse on parental decision-making for childhood immunizations to gain insight on why risk communication efforts have not been successful at improving uptake. The Public Understanding of Science (PUS) was used as a theoretical lens to guide Critical Discourse Analysis of texts from medical, pediatric, and public health journals, from which the analytic themes of Risk and Trust emerged. MMR uptake was framed mainly in terms of risk, indicating the dominance of the Deficit Model of PUS in the discourse. Future research and risk communication need to expand beyond current notions of risk; the Contextual Model of PUS can help highlight other factors that impact parental decision-making about MMR.

Keywords: risk communication
MMR
public understanding of science
critical discourse analysis
Advisor: Thompson, Alison
Resolution Enhancement of Ultrasonic Signals using Autoregressive Spectral Extrapolation

Time of Flight Diffraction (TOFD) is one of the most accurate ultrasonic methods for crack detection and sizing in pipeline girth welds. Its performance, however, is limited by the temporal resolution of the signal. In this thesis, we develop a signal processing method based on autoregressive spectral extrapolation to improve the temporal resolution of ultrasonic signals. The original method cannot be used in industrial applications since its performance is highly dependent on selection of a number of free parameters. This method is modified by optimizing its various steps and limiting the number of free parameters, and an automated algorithm for selection of values for the remaining free parameters is proposed based on the analysis of a large set of synthetic signals. The performance of the final algorithm is evaluated using experimental data; it is shown that the uncertainty in crack sizing accuracy can be reduced by as much as 80%. Furthermore, the proposed method is shown to be capable of resolving overlapping echoes; therefore, smaller cracks that have echoes that are not clearly resolved in the raw signal, can be detected and sized in the enhanced signal.

Keywords: Ultrasound
Resolution
Crack Sizing
Autoregressive
Spectrum
Non Destructive Testing
Advisor: Sinclair, Anthony
A Pattern-guided Adaptive Equalizer in 65nm CMOS

This thesis presents the design, implementation, and fabrication of a pattern-guided equalizer in a 65nm CMOS process. By counting the occurrence of 6 out of 16 4-bit patterns in the received data and utilizing their spectral content, the signal is equalized separately at fN and fN/2, where fN is half the bit rate. The design was packaged using a 64 pin Quad Flat No leads (QFN) package. Two different channels were used and the equalizer was able to open the eye for both 13dB and 17dB of attenuation at the Nyquist frequency. The adaptation performance was determined by measuring the vertical and horizontal eye openings for all possible equalizer coefficients. Measured results at 6Gb/s confirm that the adaptation engine opens a closed eye to within 2.6% of optimal vertical opening and 7% of optimal horizontal eye opening while consuming 16.8mW from a 1.2V supply.

Keywords: Adaptive Equalization
Adaptation algorithm
Clock and Data recovery
Eye monitor
Advisor: Ali, Sheikholeslami
The Role of Differential Phosphorylation of the Retinoblastoma Protein in Regulating Cell Proliferation and Elastogenesis

Previous studies suggest that the IGF-I stimulates the elastin gene transcription through the unique responsive sequence on the elastin promoter, which is a putative retinoblastoma control element (RCE). This site interacts with (Sp)-family transcription factors whose delivery is mediated by the retinoblastoma protein (Rb). Since Rb (phosphorylated on serine 780) has been implicated in the initiation of the cell cycle, we speculated that a different phosphorylation of Rb might determine Rb involvement in elastogenesis. Obtained results demonstrated that, IGF-I-induced elastogenic signaling pathway in human dermal fibroblasts includes activation of cyclinE/cdk2 causing a site specific phosphorylation of Rb on threonine 821. This permits the sequestration of Sp1 by Rb before it could bind the elastin promoter, thereby allowing the elastin gene transcription. We also found that blocking of H-Ras in Costello syndrome fibroblasts (characterized by heightened proliferation and impaired elastogenesis), selectively down-regulated Rb phosphorylation on serine 780 and normalized impaired elastogenesis.

Keywords: Elastin
Retinoblastoma protein
Cell proliferation
Retinoblastoma control element
Cyclin E
Cyclin D
cdk-2
cdk-4
Costello Syndrome
Serine 780
Threonine 821
Elastin gene promoter
Sp-1
Advisor: Hinek, Aleksander
Chromatin Reassembly following a DNA Double-Strand Break Repair: The Ctf18-complex and Ctf4 work in concert with H3K56 Acetylation

The budding yeast, Saccharomyces cerevisiae, serves as an excellent model for identifying fundamental mechanisms of DNA repair. A Local Coherence Detection (LCD) algorithm that uses biclustering to assign genes to multiple functional sub-groups was applied on the chromosome E-MAP containing genetic interactions among genes involved in nuclear processes. Using this method, we found that Asf1 and Rtt109, genes that are together required for histone H3K56 acetylation, cluster together with Ctf4, Ctf18, Ctf8 and Dcc1, genes important for efficient sister chromatid cohesion. It is known that H3K56 acetylation is required for post-repair chromatin reassembly at sites of DNA double-strand breaks (DSBs). The cohesion genes were previously implicated in the repair of some DNA DSBs, but the nature of their involvement has not been reported. The experimental data in my thesis work suggest that Ctf4, Ctf8, Ctf18 and Dcc1 function in the post-repair chromatin reassembly pathway.

Keywords: yeast
genetic interaction
DNA repair
acetylation
cohesion
histone
Advisor: Greenblatt, Jack F.
Wodak, Shoshana J.
Measurement of T1 in the Vessel Wall Using MRI

This thesis presents a high-resolution volumetric technique to measure the longitudinal relaxation time T1 in the vessel wall using MRI. The method of Variable Flip Angles (VFA) was applied using a new strategy for flip angle selection that allows measurement of T1 with high accuracy (< 10% mean error) and precision (T1-to-noise ratio > 10) over the wide range of anticipated values (300-3000ms) in the vessel wall. This strategy was validated in simulation, phantom and volunteer spinal cord experiments. Initial validation of vessel wall T1 measurements was performed in ex-vivo thoracic aorta samples from cholesterol-fed rabbits. For in-vivo vessel wall T1 mapping, the technique was augmented with spatial saturation bands for flow suppression and applied to the carotid arteries of three volunteers. Preliminary results from volunteers suggest that this approach may be useful in characterizing T1 changes associated with high-risk atherosclerotic disease.

Keywords: Magnetic Resonance Imaging
Atherosclerosis
T1
MRI
Vessel Wall
Imaging
Advisor: Moody, Alan
Disruption of the Toll-like Receptor 4 Signaling Pathway by Salmonella Effector SigD

The enteropathogenic bacteria Salmonella are the main cause of food borne gastroenteritis worldwide. The activation of Toll-like receptor 4 (TLR4) by LPS triggers an immune response to counter infection. Signaling by TLR4 requires the adaptor proteins, TIRAP and TRAM. Recruitment and activation of these molecules is dependent on the membrane lipid, PIP2. The Salmonella effector, SigD, is a 4-phosphatase that depletes PIP2 from the host plasma membrane during invasion. Thus, we investigated if SigD could lead to the interruption of the TLR4 pathway. We observed that SigD expression caused the disappearance of TIRAP from the Salmonella containing vacuoles (SCVs) in HeLa cells. Furthermore, we demonstrated that SigD attenuates NF-κB activation, implicating SigD in the disruption of the MyD88 dependent pathway. In addition, the observed inhibition of PKCε phosphorylation suggests SigD may also block the other branch of the TLR4 signaling cascade, the MyD88 independent pathway.

Keywords: Salmonella
SigD
Advisor: Terebiznik, Mauricio
Generating Reliable and Predictable Lower-Limb Torque Vectors using Functional Electrical Stimulation

Recovery of the ability to maintain balance during standing is one of the primary and essential goals of rehabilitation programs for individuals with Spinal Cord Injury (SCI). Regaining functionality during standing by means of a neuroprosthesis would decrease secondary complications and increase independence, and would consequently improve the quality of life of these individuals. However, the development of a standing neuro- prosthesis requires techniques to generate reliable and predictable torque vectors in the lower limbs. We proposed and tested a method based on surface Functional Electrical Stimulation (FES) and the idea that three independent muscles can form a basis that would span the joint torque vector space. We tested the proposed stimulation technique on the quadriceps muscles that produce knee extension. The results of this study suggest that the quadriceps muscle basis vectors are insufficient to cover the knee joint vector space.

Keywords: Functional Electrical Stimulation
Standing Balance
Advisor: Popovic, Milos R.
Rights Without Remedies: The Court Party Theory and the Demise of the Court Challenges Program

The author argues that the Court Challenges Program’s 2006 cancellation was based on claims that judicial review is undemocratic, including those made by three academics, Rainer Knopff, F.L. Morton and Ian Brodie; the Court Party Theorists (the “CPT”). Through a study of Charter equality cases, this paper examines the CPT’s arguments regarding judicial activism, interest groups and interveners and finds they are largely unsupported by statistical evidence. Further, the debate about judicial review and democracy obscures judicial review’s important auditing function over the legislature’s constitutional adherence. This audit depends on individuals’ capacity to pursue Charter litigation, an ability compromised by the access to justice crisis. The author examines this crisis and the efforts to fill the funding gap left by the CCP’s cancellation and concludes that a publicly-funded program like the CCP is best-placed to ensure that the Charter remains a relevant tool for enforcing fundamental human rights in Canada.

Keywords: Court Challenges Program
access to justice
Charter of Rights and Freedoms
equality rights
Court Party Theory
litigation
intervener
intervenor
interest groups
judicial activism
judicial review
legal aid
section 15
s. 15
Rainer Knopff
Ian Brodie
F. L. Morton
CCP
human rights
statistics
study
quantitative
Advisor: Sossin, Lorne
Intrinsic Properties of Rhodamine B and Fluorescein Gas-phase Ions Studied using Laser-Induced Fluorescence and Photodissociation in a Quadrupole Ion Trap Mass Spectrometer

Studying the intrinsic properties of molecules in the gas-phase is advantageous, since it reduces the complexity present in solution that arises from interactions between the molecule of interest and other species present in the local environment, including those with the solvent itself. In this report, the photophysical properties of gaseous cationic rhodamine B (RBH+) were determined and photodissociation reaction kinetics and power dependence of three prototropic forms of fluorescein; the cation ([F + H]+), monoanion ([F - H]-), and dianion ([F – 2H]-2), each of which possesses their own distinct spectral properties, were measured. The analyte ions of interest were formed via electrospray ionization, mass-selected and stored in a quadrupole ion trap mass spectrometer which has been customized to enable gas-phase spectroscopic studies. Knowledge of the intrinsic photophysical properties of such chromophores in the gas-phase will enable a better understanding of how the local environment of the molecule alters its properties.

Keywords: Analytical
Chemistry
Mass Spectrometry
Laser-induced fluorescence
Photodissociation
Advisor: Jockusch, Rebecca A.
Robustness Analysis of Simultaneous Stabilization and its Applications in Flight Control

Simultaneous stabilization is an important problem in the design of robust controllers. It is the problem of designing a single feedback controller which will simultaneously stabilize every member of a finite collection of liner time-invariant systems. This provides simplicity and reliability which is desirable in aerospace applications. It can be used as a back-up control system in sophisticated airplanes, or an inexpensive primary one for small aircraft. In this work the robustness of the simultaneous stabilization problem, known as the Robust Simultaneous Stabilization (RSS) problem, is addressed. First, an optimization methodology for finding a solution to the Simultaneous Stabilization (SS) problem is proposed. Next, in order to provide simultaneous stability while maximizing the stability robustness bounds, a multiple-robustness optimization design methodology for the RSS problem is presented. The two proposed design methodologies are then compared in terms of robustness of the designed controller.

Keywords: Aerospace Engineering
Flight Control
Simultaneous Stabilization
Control Robustness
Aircraft Stability and Control
Advisor: Liu, Hugh
Perez, Ruben E.