VictorJuiz/thesis huggingface dataset:
Pcl-Pho85-dependent phosphorylation of Rvs167, potential regulation of an actin cytoskeletal protein by a cyclin-dependent kinase

Genetic and biochemical experiments suggest that the Rvs167 protein regulates the yeast actin cytoskeleton by participating in multiple protein complexes. The role of Rvs167 as an adaptor protein is similar to that proposed for the Rvs167 homolog amphiphysin in clathrin-mediated endocytosis in mammalian neurons. Rvs167 has a number of protein binding domains including a single C-terminal SH3 domain that participates in at least four distinct protein interactions. Rvs167 is a phosphoprotein and the cyclin-dependent kinase (CDK) Pcl-Pho85 is thought to be an important regulator of Rvs167. Putative Pho85 phosphorylation sites were identified and mutated to test whether Pcl-Pho85-dependent phosphorylation affects Rvs167 function. I analyzed the S/T P sites in Rvs167 by phosphopeptide mapping and phosphoamino acid analysis and identified S379 as a Pho85dependent phosphorylation site 'in vivo'. S379 of Rvs167 is in the center of the neutrally charged Gly, Pro, Ala rich region of Rvs167, and may influence Rvs167 protein interactions. Phenotypic analysis of putative Pho85 phosphorylation site mutants suggests that phosphorylation at S379 does have an important function 'in vivo'.
Without a voice: A study of children who have experienced family violence

This thesis was intended to study children who have experienced family violence and to learn if their voices are sufficiently heard in this issue. Questionnaires were sent out to shelters and hostels across Ontario, and interviews with child advocates and two observations of children were done at selected facilities. Two experts were consulted concerning the findings in this thesis. Five main themes, all grounded in the data collected, are presented in this study. They include children's behaviour, socialization, funding, programs and services, and cultural implications. It was concluded that children are not heard from a societal perspective, but they are recognized by those who work with them. We need to hear these children's voices, and governments must put children on the political agenda through more funding so that qualified workers and ample resources are available to help children make the transition from violent to nonviolent environments.
Characterization of germline p53 mutations in the Li-Fraumeni Syndome

The Li-Fraumeni Syndrome (LFS) is a rare hereditary cancer syndrome in which gene carriers are at high risk to develop childhood or early onset adult cancers. The genetic abnormality held responsible for this cancer predisposition is a heterozygous germline mutation in the p53 tumour suppressor gene. This study reports one LFS patient who harbored three germline p53 alterations (R156H/R267Q on one allele and R290H on the other). In vitro assays that measure the ability of these p53 variants to suppress cellular growth and activate transcription demonstrate that each of these amino acid substitutions confers partial or complete loss of wild-type p53 function. Therefore, this individual completed embryonic development despite lacking entirely wild-type p53, which has not been reported to date in humans in vivo. This recapitulates what has been observed in p53-deficient mice, which is striking since p53 plays a central role in regulating cell cycle control and differentiation.
Hungarian church-state relationships, a socio-historical analysis

The primary goal of this socio-historical analysis is to provide a thorough account of Hungarian church-state relations. Beginning with a broad investigation of sociologists' failure to develop a comprehensive theoretical framework for studying church-state relations, the study suggests that this is due to a preoccupation with definitional difficulties and the processes of secularization and globalization. Following this, a review of relevant literature is undertaken in order to generate a set of analytical questions, which are applied in a case study of Hungarian church-state relations from 896 C. E. to the present day. Subsequent chapters discuss these relations in four distinct historical periods in which the shifting balance of power between the sacred and the secular is a central issue of both political and religious importance. Special attention is devoted to the significant intertwining of church and state during the Communist era, a situation fundamentally at odds with official government ideology and policy. An attempt is also made to explore post-Communist church-state relations in an effort to formulate some tentative predictions for the future. In this context, particular emphasis is placed upon the pivotal role of the middle class in the reconstruction of civil society and the crucial importance of the churches in a dangerous period of increasing nationalist rhetoric. A Hungarian case study suggests the need to modify two important sociological accounts of different aspects of church-state relations. David Martin's explanation (1978) of the role of established churches within societies under external threat is adjusted to fit the denominationally mixed character of Hungarian society. Similarly, Mary Gautier's analysis (1998) of the respective roles of church and state elites in the restructuring of civil society is reformulated on the basis of Hungarian evidence.
Signal transduction responses to enteropathogenic Escherichia coli and Shiga toxin-producing Escherichia coli infections

Enteropathogenic 'Escherichia coli' (EPEC) and Shiga toxin-producing 'Escherichia coli' (STEC) induce morphological changes in infected epithelial cells. The resulting attaching and effacing lesion is characterized by intimate bacterial adherence to epithelial cells with microvillus destruction, cytoskeletal rearrangement and aggregation of host cytoskeletal proteins, including Ã-actinin. This study was undertaken to further characterize host cytosolic responses to bacterial infection by using a series of specific cell signaling inhibitors of phosphoinositide-specific phospholipase C, phosphoinositide 3-kinase, 5-lipoxygenase, and cyclooxygenase. Inhibitory effects of the enzyme inhibitors were measured by their ability to disrupt formation of attaching and effacing lesions by EPEC and STEC. HEp-2 cells were preincubated with inhibitors before infection with EPEC strain E2348/69 (serotype O127:H6), STEC strain CL56 (O157:H7), or a signaling-deficient EPEC mutant UMD864 (O127:H6). Attaching and effacing lesions were detected by immunofluorescence microscopy using a monoclonal antibody against Ã-actinin. While initial bacterial adherence to epithelial cells was not reduced, Ã-actinin accumulation in infected HEp-2 cells was blocked by enzyme inhibitors ET-18-OCH 3, wortmannin, LY294002, and NDGA, but not by NS-398. This study suggests that the cytoskeletal reorganizations induced during EPEC and STEC infections are dependent on phospholipase C, phosphoinositide 3-kinase, and 5-lipoxygenase, but cyclooxygenase-independent. Understanding the signal transduction responses to EPEC and STEC infections could provide the basis for the development of novel therapies for use in the prevention and treatment of these infections in humans.
The interaction between human cytomegalovirus and polymorphonuclear leukocytes using an in vitro model of study

'Background'. Human cytomegalovirus (HCMV) is a common causative agent of congenital viral infections in humans and an important pathogen in allograft recipients. In patients with active HCMV infection, detection of, HCMV phosphoprotein pp65 inside polymorphonuclear leukocytes (PMNL) is often used to follow the virus load. It is as yet unresolved whether detection of pp65 and other viral antigens is a result of active HCMV infection within PMNL, or detection is due to uptake and accumulation of viral antigens from infected cells through phagocytosis. The purpose of this study was to investigate the interaction between HCMV and PMNL, using an 'in vitro ' model of study. 'Methods'. HL-60 cells (human promyelocytic leukemia cell line) which are of myeloid lineage, were stimulated with DMSO to obtain more mature cells. Unstimulated and stimulated cells were inoculated with live and heat inactivated HCMV at various multiplicities of infection (MOI). Cells were tested for the presence of HCMV immediate-early (IE) antigen (Ag), pp65 Ag, and late Ag by immunofluorescence assays (IFA). They were further tested for HCMV DNA, mRNA, infectious viral progeny, and examined for viral particles by electron microscopy (EM) and immunoelectron microscopy (IEM). (Abstract shortened by UMI.)
An institutional ethnography of elder care: Understanding access from the standpoint of ethnic and "racial" minority women

The current study addresses the problematic of access for ethnic and "racial" minority elderly women through an examination of the working processes of a publicly-funded organization which provides elder care services in Ontario, from the standpoint of these women. Using Institutional Ethnography methodology, the study explicates how state ideologies become infused into the operating policies and guidelines of elder care ideologies become infused into the operating policies and guidelines of elder care organizations, shaping the actual way access is both conceptualized and operationalized and the subsequent interactions between workers and their elderly clients. Interviews conducted with 43 participants (elderly women, agency staff, community agency and institutional staff) explicate how actors both reproduce relations of oppression and counter or resist those relations. Several problematic constructions have been identified which highlight the disjunctures between older women's expressed desires regarding access and the way access to services are operationalized in elder care agencies. These include: family involvement in care; prolonged engagement as a means of facilitating access; the influence of acute health care models on eligibility for and delivery of care; and, a focus on cultural competence and language in the delivery of ethno-specific services. Program and practice implications are highlighted in order to shift the dominance of state-orchestrated ideologies which operate to shape the experience of women who are multiply situated on the margins and who have been and continue to be oppressed within elder care institutions.
Air Pollution Control of Trace Contaminants Using Electron Beam Irradiation

This thesis introduces a new approach for the control of air pollutant emissions. This technique uses electron beam radiation and ammonia (NH 3) injection to simultaneously oxidize organic pollutants and reduce nitrogen oxide (NO). The objective of this research is to develop a numerical chemical kinetic model to evaluate this technique's effectiveness. Benzene (C6H 6) and ethyl chloride (C2ClH5) were selected as candidate pollutants representing aromatic and chlorinated hydrocarbons, respectively The model is built upon reaction mechanisms for benzene, ethyl chloride, nitric oxide and electron beam. The destruction efficiency of the contaminants was evaluated as a function of temperature, residence time, beam dose and initial concentration. Pollutant reduction effectiveness was evaluated over a wide range of conditions, entailing the execution of the following operations: the model was run for a range of temperatures between 500 and 1500K, residence times from 0.01 to 0.4 sec and dose rates between 0.01 and 1000 kGy/s. (Abstract shortened by UMI.)
The intercourse of letters: Transatlantic correspondence in early Canada, 1640-1812

This thesis is about the process of transatlantic letter-writing between Canada and Europe from the 1640s to the War of 1812. In this era, before the advent of mass communications in the mid-nineteenth century, there was little or no postal service; communications depended largely upon commercial shipping; correspondents played an active role in the transmission of the mails; the seasonal limits on navigation in the Saint Lawrence shaped patterns of communications; letters took months to cross the Atlantic; and correspondence was vulnerable to delay and loss. Focusing on the evidence provided by the letters themselves, this thesis reveals a process that was, despite the limitations on communications, complex, rich, dynamic, and often remarkably effective. This study argues, in the first instance, that the effectiveness of the early mails can only usefully be evaluated in the context of contemporary expectations. Correspondents distinguished clearly between good and bad years, and it is from their perspective that we need to assess the frequency of opportunities by which they could write, the spacing of these opportunities over time, the length of the communications season, and the riskiness of conditions in a particular year. Second, conditions for communications were not static: they varied significantly from year to year, but also improved dramatically over time--most significantly after the Conquest when the rigid seasonal constraints of the French regime were broken. Third, the pre-institutional structures on which the mails depended were remarkably effective. Individual letter-writers developed forms and conventions of letter-writing that minimized the consequences arising from the loss or delay of letters, and managed their correspondence in ways that maximized the opportunities to write. Moreover, correspondents operated within a culture of communications that linked elite correspondents and members of the broader community in a complicated web of mutual obligation and assistance. The rules of this system were tacit rather than explicit, its processes the product of convention rather than of legislation. But for all that it was remarkably effective.
Brain regions involved in the acquisition and reversal of tone-visual associations in humans, a PET study

Positron emission tomography (PET) was used to identify brain regions that showed changes in regional cerebral blood flow (rCBF) as subjects participated in voluntary response differential conditioning study. Two tones (T1 and T2) served as the conditioned excitor (CS+) and conditioned inhibitor (CS$-$) and a visual stimulus served as the unconditioned stimulus (UCS). In phase one of the experiment T1 was the CS+ and T2 the CS$-$. In phase 2 of the experiment the contingencies were reversed. Sixteen subjects (9 males and 7 females) between the ages of 19 and 35 participated in this study. Subjects were told to press a button with their right index finger each time they were presented with the UCS. Eight PET scans were obtained from each subject. The scans were obtained while subjects were being presented with predictive CS+ and UCS trials or while subjects were presented with nonpredictive CS$-$ and UCS trials. The scan types were alternated across the experiment and four scans were obtained during each of the phases. During the acquisition of the phase two association subjects engaged a network of brain regions consisting of: right middle frontal gyrus, right inferior parietal lobule, right precentral and postcentral gyri, and right precuneus. In addition a network of brain regions involved in extinction to the first association during phase two was identified. This network consisted of right middle frontal gyrus, right thalamus, left inferior parietal lobule, and left cerebellum. Right hippocampal gyrus, right middle occipital gyrus and left middle temporal gyrus were found to involved in both learning the second association and extinction of the first association. The analysis did not identify any significant patterns of brain activation related to phase one of the experiment. (Abstract shortened by UMI.)
Moisture effects on high performance polymer composites

Fiber reinforced composites have been gaining wide use in the variety of applications such as military and aerospace. The performance of these composites may suffer when the material is exposed to adverse environments for long periods of time. In this research, diffusion of moisture into glass-epoxy, carbon-epoxy and carbon-polyimide composites immersed in 60°C and 90°C water was investigated. Also the effect of the residual stresses on the absorption behavior of the cross-ply composites was studied. In addition, the author investigated the effect of the hot and moist environment on the notched tensile strength of various types of cross-ply composites. Results of this research indicated that the notched strength of cross-ply glass-epoxy composites decreased by about 65% after 7000 hours immersion in 90°C water. In another experiment, the transverse test was used to investigate the kerosene effect on the interface of the unidirectional composites. It was found that the interface of unidirectional carbon-polyimide laminates was unaffected even after 3500 hours of 80°C kerosene immersion.
MRMA, a multicast resource management architecture for IP platforms

Multicast with QoS guarantee is a resource-efficient transmission scheme for the delivery of multi-participant multimedia applications. In this thesis, we present MRMA a multicast resource management architecture designed to provision multicast service with QoS guarantee over the IP platform. Unlike most other related work, which focuses primarily on the algorithmic or protocol aspects of the multicast problem, our work focuses on the architecture and implementation aspects pertaining to the setup and configuration of multicast sessions with QoS guarantees. MRMA offers a flexible platform for integrating the necessary components for implementing the key multicast functions that are needed for creating, maintaining and terminating multicast sessions: route selection, address allocation, session advertisement, and QoS-sensitive tree construction. MRMA implements a hierarchical architecture that features centralized monitoring of network state and semi-distributed per-session multicast management which allows it to achieve a good balance between accuracy and scalability.
A survey of monitoring practice of anesthesiologists during cardiovascular surgery

The objective, of this study was to describe monitoring practice of anesthesiologists during cardiovascular surgery, by comparing the application of two technologies, the pulmonary artery catheter (PAC) and the transesophageal echocardiogram (TEE), and by examining the application of the PAC in depth. This study consisted of an anonymously completed mailed survey of 345 practising anesthesiologists, designed to describe their practice regarding the PAC and TEE, to assess various factors associated with the use of these technologies, to define the appropriateness of PAC application in specific clinical scenarios, and to determine respondent knowledge base. Seventy-six percent of the sample responded. The PAC was more commonly used than the TEE, and both were more commonly used in academic cardiac practice. Respondents agreed strongly about the appropriate application of the PAC in patients with heart failure (ventricular impairment) but disagreed or were uncertain in many others. Patient-related factors exceeded the importance of practice-related factors in the judgement of most respondents. Specific PAC capacities were used reasonably consistently although some variation existed. Knowledge- base of PAC-related questions correlated with country of training and certification, I and type of practice, but did not correlate with the frequency of use nor type of surgery.
Cohen and random reals

We study the influence of the fundamental forcing notions, Cohen forcing and random forcing, on various combinatorial properties. We formulate variants of the cardinal characteristics <math> <f> <ge>f</ge></f> </math>, <math> <f> <ge>p</ge></f> </math>, and <math> <f> <ge>t</ge></f> </math> in terms of families of measurable functions, in order examine the effect upon these cardinal characteristics of adding one random real, and thereby prove that both statements [kappa] < <math> <f> <ge>p</ge></f> </math> and [kappa] < <math> <f> <ge>t</ge></f> </math> are preserved under the addition of one random real. We also give reformulations of the characteristics <math> <f> <ge>p</ge></f> </math> and <math> <f> <ge>t</ge></f> </math> in terms of families of Borel functions, and apply this to prove that <math> <f> <ge>t</ge></f> </math> is invariant under the addition of one Cohen real. Jensen's Theorem that Souslin's Hypothesis is consistent with CH is extended by showing that the statement 'Souslin's Hypothesis holds in any forcing extension by a measure algebra' is consistent with CH. In doing so we prove that a variation of the principle (*) (see [AT97] and [Tod99a]) for closed sets of ordinals is consistent with GCH. Silver's Theorem that, assuming the existence of an inaccessible cardinal, the failure of Kurepa's Hypothesis is consistent with CH is extended by showing that the statement 'Kurepa's Hypothesis fails in any forcing extension by a measure algebra' is consistent with CH. We give an answer to a problem of Woodin by showing that under MA all ([omega]1,[omega]1), gaps are indestructible in any forcing extension by a separable measure algebra. This is applied to obtain new results in Descriptive Set Theory concerning asymmetries between Baire category and Lebesgue measure.
Changing the subject, objectivity, trickster and the transformation of the Western academy

In this discussion, the supervaluation of scientific objectivity is critiqued in the context of its foundational role in the legitimization/delegitimization of knowledges. It is proposed that the quest for an 'absolute' objectivity constitutes a dominant Western strategy employed to maintain inequitable power relations over and against identified 'Others', and that therefore a powerful critique must be applied. It is also maintained that this paradigm informs the Western academy and thus shapes all educational experiences in the West. Transforming the dominant academy then into one which seeks to address a global spectrum of human knowledge requires strategies grounded in a powerful dialectical critique of dualistic objectivity. This critique may be found in the extant traditional and contemporary knowledges of indigenous peoples, particularly in the 'trickster' narratives, the particular characteristics of which, I contend, constitute a profound challenge to, and subversion of, narrow quests for an absolute objectivity.