VictorJuiz/thesis huggingface dataset:
American Impotence: Narratives of National Manhood in Postwar U.S. Literature

“American Impotence” investigates a continuity between literary representations of masculinity and considerations of national identity in the works of five postwar novelists. In particular, I illustrate the manner in which Ralph Ellison’s Invisible Man, John Updike’s Couples, Robert Coover’s The Public Burning, Joan Didion’s Democracy and The Last Thing He Wanted, and Bret Easton Ellis’s American Psycho challenge the patterns of daily life through which a single figure is imagined to be the essential agent of American polity: namely, the self-made individualist, characterized by manly virtues like dominance, aggression, ambition, mastery, vitality, and virility. More specifically, this project examines the manner in which the iconicity of men helps sustain a narrative of “imperilled masculinity” that at once privileges an impossible identity, situated in the representative nucleus of postwar democracy, and forecloses other modalities of political life. Observing the full meaning of the word “potency,” I elucidate the interrelationships between narrative forms, masculine norms, and democratic practice. Ellison’s work ties the maturation of African American boys to the impossibility of full participation in civic life, for instance, while in Updike’s Couples the contradictions of virile manhood manifest in the form of a fatalism that threatens to undo the carefully cultivated social boundaries of early sixties bohemianism; in a variety of ways, The Public Burning and American Psycho represent the iconic nature of masculinity as a psychic threat to those men closest to it, while Didion’s female protagonists find themselves flirting with the promises of a secret agency linked to imperial adventures in Southeast Asia and Central America. In the cultural context of the Cold War, these novelists demonstrate how intensified participation in national fantasies of potency and virility is inevitably disempowering; as an alternative, this dissertation seeks to consider impotence as dissensus detached from the mandates of hegemonic masculinity.

Keywords: National Manhood
Politics
Masculinity
Postwar
Novel
Ralph Ellison
John Updike
Robert Coover
Bret Easton Ellis
Joan Didion
Advisor: Cobb, Michael
The Role of Chloride Channels in Regulation of Pulmonary Artery Smooth Muscle Cell Proliferation

Pulmonary arterial hypertension (PAH) is a rare but fatal disease with an annual mortality rate of 15% despite current therapies. Uncontrolled proliferation of pulmonary artery smooth muscle cells (PASMCs) results in adverse vascular remodeling contributing to PAH. Understanding the mechanisms of PASMC proliferation may identify new targets for treatment. Chloride currents/channels (ICl) are expressed in PASMCs and their roles in proliferation have been suggested based on their importance in resting membrane potential and cell volume regulation. The present study explored the role of ICl in proliferation in rat and human PASMCs. We found that either nonspecific ICl inhibitors (DIDS or NPPB) or a putative specific blocker of swelling-activated ICl (ICl,swell) reduced proliferation of PASMCs cultured in serum-containing media. Patch-clamp studies showed that proliferating PASMCs had increased baseline ICl and ICl,swell in association with depolarized membrane potentials. Quantitative real-time RT-PCR studies identified expressions of CLC-3, a candidate gene of ICl,swell, and several other CLC genes in proliferating PASMCs. While selective knockdown of CLC-3 with lentiviral shRNA reduced PASMC proliferation, it had no effect on ICl,swell. These findings are consistent with the conclusion that ICl regulate proliferation of PASMCs and suggest that selective ICl inhibition may be useful in treating pulmonary arterial hypertension.

Keywords: Pulmonary arterial hypertension
Pulmonary artery smooth muscle cell
Proliferation
Chloride channel
Cell volume
Membrane potential
RNA interference
Lentivirus
CLC channels
CLC-3
DIDS
DCPIB
Advisor: Backx, Peter
Ward, Michael
The Characterization of Nemadipine and Migrazole as Small Molecule Tools for Use in the Nematode Caenorhabditis elegans

Small molecules are powerful reagents for biological investigation. They provide an alternative to genetic perturbation and may offer more control over a target’s activity. C. elegans has recently gained prominence as a platform to discover new chemical tools. Through large-scale screens for compounds that induce phenotypes consistent with the disruption of conserved pathways, we identified two previously uncharacterized molecules of interest that we named nemadipine and migrazole. Here, I describe my efforts to understand their mechanism of action. Nemadipine is structurally analogous to 1,4-dihydropyridines (DHPs), which target the Cav1 calcium channel and are used clinically to lower blood pressure. Phenotypic and genetic evidence suggest that nemadipine targets the worm Cav1 channel, EGL-19. To identify the target of nemadipine in an unbiased manner, I performed a forward genetic screen for mutants resistant to its effects. The majority of the mutants from my screen had polymorphisms in EGL-19, providing additional evidence that it is the target of nemadipine. I also found that nemadipine is the only DHP that robustly elicits phenotypes in the worm. Therefore, I used this unique chemical to investigate the in vivo interactions between DHPs and the Cav1 channel. I identified residues in EGL-19 important for DHP-sensitivity in worms and showed that some of these residues are also important for mammalian DHP-interaction. Other labs have since exploited nemadipine’s in vivo properties to demonstrate new biological insights for EGL-19. Chemical genetic analyses indicated that migrazole disrupts multiple signal transduction pathways. This, together with experiments that I performed in yeast, suggests that migrazole may affect multiple pathways by perturbation of protein transport. To identify migrazole’s target, I performed a forward genetic screen for mutants resistant to migrazole’s effects. However, I was unable to identify the target of migrazole through analysis of the mutants I isolated. This result illustrates that while forward genetic screens can be very successful for target identification, their effectiveness is likely dependent on the nature of the compound-target interaction. My work shows that all aspects of developing a small molecule into a tool for biological analysis, from its discovery to its characterization, can be accomplished using C. elegans.

Keywords: small molecules
C. elegans
nemadipine
migrazole
Advisor: Roy, Peter John
Brechtian Cinemas: Montage and Theatricality in Jean-Marie Straub and Danièle Huillet, Peter Watkins and Lars von Trier

In this dissertation I investigate the stylistic shift in the cinema of selected filmmakers whose work is rooted in Bertolt Brecht’s dramatic theory: Jean-Marie Straub and Danièle Huillet, Peter Watkins, and Lars von Trier. Through the work of these filmmakers, I trace the ongoing change in the cinematic applications of the theory. By and large, the change consists of a lessening of the role of montage – a technique that occupies a paramount place in Brecht’s theatrical and filmic practice – in favour of the objects within the camera’s field of view and the sounds within the microphone’s range. Since the ultimate effect for which the Brechtian filmmaker aims is that of Verfremdung, theatrical stylisation – itself estranging within the context of cinema – appears as a natural corollary of the described shift in emphasis. I also suggest a causal connection between the aforementioned shift and the growing self-consciousness of the style employed by meainstream cinemas (of which Hollywood is the foremost representative), which often manifests itself in the use of unorthodox editing patterns. Accordingly, I propose that we can attribute the contemporary Brechtian filmmaker’s growing reliance on mise-en-scène elements as a source of Verfremdung largely to the major film industries’ adoption of montage and other specifically cinematic codes that make a film’s style overt. Not surprisingly, perhaps, the Brechtian filmmaker – whose political stance is inherently antagonistic to that exemplified by most mainstream cinema, reacts to the normalisation of foregrounded film style by employing the opposite strategy.

Keywords: Bertolt Brecht
Jean-Marie Straub
Danièle Huillet
Peter Watkins
theatricality
montage
Advisor: Keil, Charlie
Soldovieri, Stefan
Bridging the Genomics Gap: The role of Large-scale Genotyping Projects in the Developing World and the Importance of Genomic Sovereignty

In recent years, there have been several proposals for large-scale human genotyping projects in the developing world. The dissertation presented here explores the motivations, opportunities and challenges of initiating locally led, large-scale genotyping projects documenting human genomic variation in the developing world. I analyze two case studies: the Indian Genome Variation Consortium in India and the University of Cape Town, Department of Human Genetics and the African Genomics Education Initiative in South Africa. These case studies, together with similar projects in Mexico and Thailand provide compelling reasons for pursuing these projects: the potential to address local health needs and reduce health care costs; the opportunity to stimulate economic development through investments in genomic sciences, and the availability of unique population resources. In an effort to capture the value of these investments and promote an equal stake in international collaborations, Mexico and India have developed guidelines and laws to protect local human genetic material as a sovereign resource, referred to here as ‘genomic sovereignty’. Critics have suggested that it can impede international collaborations and reduce access to external funding. I provide an in depth analysis of genomic sovereignty and how it may contribute to each country’s aim of achieving health equity through investments in genomics, its relation to heritage and patrimony, and its potential limitations. The debate is critical, as the knowledge generated from large-scale human genomic research will need to be interpreted in larger international collaborative efforts before it can lead to health benefits. Qualitative case study methodology is employed and the primary data source consists of interviews conducted with key informants. The research described here provides a source of empirical description and analysis that is informing the framing of policies, principles and practices on how research infrastructure and capacity are being established for human genomic sciences in developing countries.

Keywords: genomics
sovereignty
science & technology
innovation
qualitative
case study
Advisor: Daar, Abdallah
Nineteen Thirty-four: Generic Hybridity and the Search for a Democratic Aesthetic

This dissertation poses a fundamental question: why does a concern about the value of literary writing emerge during a felt crisis in public speech, especially in times of war? My focus is 1934, the year that Hitler became Führer and Socialist Realism was formulated in the USSR. In this year, Mikhail Bakhtin and Erich Auerbach developed the foundations of their narrative theory, while Virginia Woolf, T. S. Eliot, and Wyndham Lewis embarked on new ventures in genre. Despite their differences, I argue, these writers all sought to challenge the linguistic basis of political power by developing new forms that engage readers in independent, active ways. Woolf, Eliot, and Lewis responded to totalitarianism with experiments in generic hybridity, believing that inter-generic dialogue could promote social dialogue and democratic modes of thought. My introduction analyses the political and aesthetic implications of German and Soviet propaganda in 1934. Chapter 1 establishes a theoretical frame through two works written in exile from these totalitarian regimes. Bakhtin’s “Discourse in the Novel” and Auerbach’s Mimesis both posit the centrality of genre to political freedom, but Bakhtin’s binary of monologic poetry and dialogic prose contrasts with Auerbach’s arguments for the political potential of mixed forms. Chapter 2 shows how similar concerns about pluralist voicing inform debates concerning the “death of poetry” in England; nonetheless, I conclude that the political poetry of Thirties Poets often privileged ideology over dialogism. Three chapters then explore the emergence of hybrid genres in Woolf, Eliot, and Lewis. I argue that Woolf’s unpublished “Ode to Cutbush” is a prose-poetic hybrid that employs poetic rhythm and free indirect discourse to instill democratic values of empathy and tolerance; that Eliot’s first completed play, The Rock, invokes the ideological complexities and unresolved political contradictions of his journalism and editorial practice to incite the audience’s active collaboration; and that Lewis’s novelistic poem One-Way Song attempts to escape the increasingly monologic voice of his political writings and return to the polyphonic style and polyvalent thinking of his earlier work. My conclusion demonstrates these writers’ continuing commitment to the democratic potential of generic hybridity during World War II.

Keywords: 1934
Democracy
Generic Hybridity
Advisor: Cuddy-Keane, Melba
Disambiguating Rebirth: A Socio-rhetorical Exploration of Rebirth Language in 1 Peter

Rebirth language has traditionally been associated with the initiation rite of baptism and relegated to discussions within this limited framework. Analyses of 1 Peter—where rebirth language is particularly dominant—have focussed almost exclusively on a baptismal framework for understanding this language. However, a detailed reading of the letter does not reveal any association between rebirth and Christian rites of initiation. Whatever action, activity or idea triggered the use of this language, its role in the letter has never been adequately explored. This study employs socio-rhetorical analysis to examine the role of rebirth language within the letter of 1 Peter and within its larger cultural and textual context. Rebirth language is employed in the key opening section of the letter and, within the framework of familial language, serves as a central distinctive of the letter’s recipient-focussed argument. As part of the familial metaphor, rebirth highlights the readers’ identity as children whose πατήρ (“father”) is God. A comprehensive analysis of all other extant (first century) texts employing rebirth language, reveals that, while 1 Peter’s use of such language shares some points of contact with other expressions of rebirth, the meaning of rebirth in 1 Peter is not directly tied to any related language. More likely, 1 Peter contains cultural allusions to the developing idea of rebirth that is also shared—in different ways—with other extant materials. No other source, however, contains the same usage and implied meaning of rebirth language as 1 Peter. Instead, 1 Peter’s author, building upon the powerful father-child analogy, intends to shape his readers’ self-perceptions using this language to provide a sense of identity without encouraging extensive alienation from the larger society. 1 Peter’s use of rebirth language builds upon and intensifies the cultural familial metaphor in order to help firmly establish the recipients’ Christian identity in the midst of their associations and interactions within their social context.

Keywords: 1 Peter
rebirth
socio-rhetorical
Advisor: Richardson, Peter
Vice and Self-examination in the Christian Desert: An Intellectual Historical Reading of Evagrius Ponticus

This thesis offers an analysis of the vice tradition of the fourth-century monk Evagrius Ponticus. While Evagrius, like others before him, understands that virtue and vice have an affective component, and that these affections are reactions to mental images, for Evagrius these images are veridically thinner than what we find in earlier discussions of passion in ancient philosophy. As a result, vice is less a matter of false reasoning and false perception than it is a matter of the excessive dwelling on representations connected with events of one’s personal history, to the point that the passions aroused at the time of those events become globalized dispositions. Evagrius’s concern with how memories lead us to dwell on these “bad thoughts” proves to be point of contact with psychoanaly which many modern authors, including Michel Foucault, have detected; yet a close analysis of what Evagrius takes to be involved in self-examination reveals that Foucault’s account of the “technologies of the self” fails to take into account Evagrius’s interest in the distinction between the endowed self, that self which is examined, and the ideal self, the goal of the ascetic activity.

Keywords: Evagrius Ponticus
moral psychology
Advisor: Sinkewicz, Robert
Preventing Suicides in the Toronto Subway System: A Program Evaluation

Despite the wealth of information on suicide prevention issues and the widespread implementation of suicide prevention strategies, program evaluation efforts have been limited. Lack of sound program evaluation remains one of the most significant barriers to identification and implementation of effective intervention and prevention strategies. The purpose of this study was two-fold: to conduct a summative evaluation of the gatekeeper suicide prevention program implemented at the Toronto Transit Commission, and to concomitantly, appraise the efficacy and effectiveness of the Kirkpatrick evaluation model as an analytical framework to guide suicide prevention program evaluations. The study used a two-phase, sequential mixed-method approach of converging quantitative and qualitative methodologies. The quantitative study employed a repeated measures design and examined the immediate and long-term effects of the gatekeeper program on attitudes, knowledge, intervention abilities. The qualitative study consisted of semi-structured interviews and explored participants’ effective and utility reactions to the gatekeeper training program. The results of this study indicated safeTALK and suicideAWARE training programs increased participants’ knowledge of suicide and suicidal behaviour, enhanced positive attitudes toward the suicidal individual, suicide intervention, and improved intervention skills. The empirical findings from this study support the premise that the Kirkpatrick evaluation model could be adapted for use in gatekeeper program evaluations. The model provides a highly relevant, well-rounded, rigorous approach to suicide prevention program evaluations.

Keywords: suicide prevention
program evaluation
Advisor: Links, Paul
Function and Regulation of Septins During Mammalian Cell Division

Septins are a family of GTP-binding proteins implicated in mammalian cell division. Since these proteins form heterologous complexes and filaments in interphase cells, it has been assumed that depletion of any or all septins in a given cell type will give rise to the same phenotype. I demonstrate that while all septins expressed in HeLa cells localize to the cleavage furrow and midbody during cytokinesis, and co-immunoprecipitate throughout cell division, they do not all have identical roles during this process. Specific depletion of SEPT2 or SEPT11 caused defects in the early stages of cytokinesis, ultimately resulting in binucleation. Similar results were observed upon simultaneous depletion of all septins. In sharp contrast, SEPT9 was dispensable for the early stages of cell division, but was critical for the final separation of daughter cells. I demonstrate that SEPT9 mediates the localization of the vesicle-tethering exocyst complex to the midbody. Immunofluorescence microscopy suggests that SEPT9 may act to compartmentalize the exocyst at the site of abscission, analogous to the role performed by septins in Saccharomyces cerevisiae. I provide evidence that the N-terminal region of SEPT9, which is absent from the shorter SEPT9 isoforms, plays an important role in abscission. I describe a long-anticipated link between a mammalian septin and the cell cycle machinery by showing that the N-terminal region of SEPT9 is phosphorylated at threonine 24 upon mitotic entry by cyclin-dependent kinase 1. This creates a binding site for the WW domain of the peptidyl-prolyl isomerase Pin1. I provide evidence that Pin1 induces a conformational change in the N-terminal region of SEPT9 that is important for the completion of cytokinesis. I propose that mitotic regulation of SEPT9 by Cdk1 and Pin1 regulates an interaction between SEPT9 and an unidentified protein that is critical for abscission.

Keywords: cytokinesis
septin
phosphorylation
SEPT9
Advisor: Trimble, William S.
The Transgressive Stage: The Culture of Public Entertainment in Late Victorian Toronto

“The Transgressive Stage: The Culture of Public Entertainment in Late Victorian Toronto,” argues that public entertainment was one of the most important sites for the negotiation of identities in late Victorian Toronto. From the vantage point of the twenty-first century, where theatre is strictly highbrow, it is difficult to appreciate the centrality of public entertainment to everyday life in the nineteenth century. Simply put, the Victorian imagination was populated by melodrama and minstrelsy, Shakespeare and circuses. Studying the responses to these entertainments, greatly expands our understanding of Victorian culture. The central argument of this dissertation is that public entertainment spilled over the threshold of the playhouse and circus tent to influence the wider world. In so doing, it radically altered the urban streetscape, interacted with political ideology, promoted trends in consumption, as well as exposed audiences to new intellectual currents about art and beauty. Specifically, this study examines the moral panic surrounding indecent theatrical advertisements; the use by political playwrights of tropes from public entertainment as a vehicle for political satire; the role of the stage in providing an outlet for Toronto’s racial curiosity; the centrality of commercial amusements in defining the boundaries of gender; and, finally, the importance of the theatre—particularly through the Aesthetic Movement—in attempts to control the city’s working class. When Torontonians took in a play, they were also exposing themselves to one of the most significant transnational forces of the nineteenth century. British and American shows, which made up the bulk of what was on offer in the city, brought with them British and American perspectives. The latest plays from London and New York made their way to the city within months, and sometimes weeks, of their first production. These entertainments introduced audiences to the latest thoughts, fashion, slang and trends. They also confronted playgoers with issues that might, on the surface seem foreign and irrelevant. Nevertheless, they quickly adapted to the environment north of the border. Public entertainment in Toronto came to embody a hybridized culture with a promiscuous co-mingling of high and low and of British and American influences.

Keywords: public entertainment
commercial amusements
gender
race
class
political history
advertising
circus
J. W. Bengough
Blackface Minstrelsy
Sarah Bernhardt in Canada
Oscar Wilde in Canada
aesthetic movement
theatre
cultural history
Victorian Toronto
Canadian History
Antisemitism
Advisor: Radforth, Ian