VictorJuiz/thesis huggingface dataset:
Teaching with the Flesh: Examining Discourses of the Body and their Implication in Teachers' Professional and Personal Lives

This dissertation examines how teachers understand and use their own bodies in their everyday practice of teaching. Using a poststructural theoretical framework and an ethnographic and arts-based research methodology, I demonstrate how discourses of the body shape experiences of teaching and teachers’ lives. This work is significant not only because it has direct implications for teachers but also because teachers’ bodies are rich and complex sites for theorizing and thinking critically about contemporary practices and discursive understandings that shape our lives. I call the research methodology that I used in this study “embedded performed ethnography”. This methodology involved in-depth ethnographic interviews, creative writing, and dramatic performance with twelve teachers in Ontario. By drawing on three distinct but interrelated fields: critical physical education, feminist and queer curriculum theory and Fat Studies, my research demonstrates the richness and complexity of teachers’ professional lives and the impact that dominant discourses of the body have on educational spaces. I use three key concepts to analyze the experiences and writing of the research participants. First, I use the concept of ‘biopedagogy’ to examine the ways in which teachers’ bodies are subject to regulation and policing in schools. Next, I use the concept of ‘performance’ to examine how participants use their bodies to construct and reproduce dominant notions of health in the classroom. Lastly, I use ‘affect’ as a concept to address the complex and complicated moments that occur on and through a teacher’s body in the classroom. I work with the everyday experiences of teachers in the classroom to explore how particular teaching moments illustrate and connect to the broader discourses and practices of the body that shape our lives.

Keywords: the body
discourse
teaching/teaching experiences
arts-based methodology
performed ethnography
Advisor: Goldstein, Tara
Potential Precursors of Comorbidity: Examining how Emotions, Parental Psychopathology, and Family Functioning Relate to Depressive Symptoms in Young Anxious Children

Objective: Past research indicates that comorbid anxiety and depression in youth is associated with greater functional impairment than anxiety alone. To elucidate those factors which may increase vulnerability to depressive disorders, the current study examined several clinical correlates (i.e., feelings ratings, parental psychopathology symptoms, and family functioning) of comorbid depressive symptoms in young anxious children. Method: Sixty-eight children, aged 6 to 10 years (M = 9.06, SD = 1.10), and caregivers completed measures assessing child depressive symptoms. Furthermore, children completed self reports of anxiety symptoms, feelings ratings, and family functioning, while caregivers completed self reports of psychopathology symptoms and family functioning. Predictors of child depressive symptoms were examined separately for girls and boys. Results: In females, hierarchical regression analyses revealed that, after controlling for anxiety, higher sadness and lower positive feelings accounted for 30% of variance in child-reported depressive symptoms. Further analyses indicated that child-reported overall family dysfunction moderated the relationship between positive feelings and depressive symptoms, such that high family dysfunction increased the risk of depressive symptoms in females with low positive emotions. In males, hierarchical regression analyses revealed that, after controlling for anxiety, higher negative/hostile feelings and child-reported overall family dysfunction accounted for 19% of variance in child-reported depressive symptoms. Further analyses of family functioning in males revealed that child-reported family cohesion and conflict were negatively and positively correlated, respectively, with depressive symptoms. Family dysfunction did not moderate the relationship between feelings ratings and depressive symptoms. The only significant predictor of caregiver-reported child depressive symptoms, for males only, was caregiver self-reported overall psychopathology symptoms. Further analyses indicated that, for males, caregiver depression and hostility symptoms correlated positively with caregiver-reported child depressive symptoms. Conclusions: Different patterns of emotion and family functioning predicted self-reported depressive symptoms in males and females. Self and caregiver reports of child depressive symptoms were not related, with only caregivers’ psychopathology symptoms predicting their reports of child depressive symptoms. Results suggest the importance of assessing child-reported feelings and family dysfunction, and parental symptomatology, of clinically anxious children. To prevent future depressive disorders in these children, different targets of intervention for males and females may be warranted.

Keywords: anxiety
depression
comorbidity
subthreshold symptoms
youth
children
emotions
feelings ratings
parental psychopathology
family functioning
Advisor: Manassis, Katharina
Nanopatterned Polymer Coatings for Marine Antifouling Applications

Marine biofouling is the accumulation of marine species on surfaces submerged in seawater leading to unwanted problems for man-made surfaces such as hulls of ships and aquaculture nets. Historically, the amount of biofouling was regulated using metal based coatings whose usage have been disused lately due to adverse toxic effects. Alternative environmentally friendly coatings are currently avidly being pursued. Nanopatterned polymer thin films were investigated as potential candidates for marine antifouling coatings. Polystyrene-block-poly(2-vinyl pyridine) and polystyrene-block-poly(methyl methacrylate) diblock copolymer thin films self-assembled using vapor solvent annealing into cylinders perpendicular to the substrate composed of poly(2-vinyl pyridine) or poly(methyl methacrylate) respectively with diameters between 30 nm to 82 nm and center-to-center spacing between 46 nm to 113 nm in a polystyrene matrix on various substrates such as silicon or nylon. Polystyrene-block-poly(2-vinyl pyridine) copolymers were also mixed with the photoinitiator benzophenone and irradiated with ultraviolet light to crosslink the polymer chains and decrease the surface hydrophobicity. In the case of polystyrene-block-poly(methyl methacrylate), the yield of these nanopatterned films increased with the modification of the vapor annealing method. A low temperature vapor annealing technique was developed in which the annealing occurs at 2 °C. In another strategy, polystyrene and poly(2-vinyl pyridine) homopolymers were nanopatterned with alternating lines and grooves with widths between 200 nm and 900 nm and depths between 15 nm to 100 nm using Thermal Nanoimprint Lithography. Poly(2-vinyl pyridine) films were synthesized as brushes using surface initiated Atom Transfer Radical Polymerization to produce robust polymer films. The chemical and/or the topographical heterogeneity of the polymer surfaces influenced the settlement of Ulva linza algae zoospores. Overall, the incorporation of nanoscale features enhanced the antifouling properties of the samples. Further exploration of these types of coatings is highly encouraged.

Keywords: marine biofouling
diblock copolymers
AFM
nanopatterned polymer films
Advisor: Walker, Gilbert C.
The Involvement of Ventral Tegmental Area Dopamine and CRF Activity in Mediating the Opponent Motivational Effects of Acute and Chronic Nicotine

A fundamental question in the neurobiological study of drug addiction concerns the mechanisms mediating the motivational effects of chronic drug withdrawal. According to one theory, drugs of abuse activate opposing motivational processes after both acute and chronic drug use. The negative experience of withdrawal is the opponent process of chronic drug use that drives relapse to drug-seeking and -taking, making the identification of the neurobiological substrates mediating withdrawal an issue of central importance in addiction research. In this thesis, I identify the involvement of the neurotransmitters dopamine (DA) and corticotropin-releasing factor (CRF) in the opponent motivational a- and b-processes occurring after acute and chronic nicotine administration. I report that acute nicotine stimulates an initial aversive a-process followed by a rewarding opponent b-process, and chronic nicotine stimulates a rewarding a-process followed by an aversive opponent b-process (withdrawal). These responses can be modeled using a place conditioning paradigm. I demonstrate that the acute nicotine a-process is mediated by phasic dopaminergic activity and the DA receptor subtype-1 (D1R) but not by tonic dopaminergic activity and the DA receptor subtype-2 (D2R) or CRF activity, and the opponent b-process is neither DA- nor CRF-mediated. I also demonstrate that the chronic nicotine a-process is DA- but not CRF-mediated, and that withdrawal from chronic nicotine (the b-process) decreases tonic but not phasic DA activity in the ventral tegmental area (VTA), an effect that is D2R- but not D1R-mediated. I show that a specific pattern of signaling at D1Rs and D2Rs mediates the motivational responses to acute nicotine and chronic nicotine withdrawal, respectively, by demonstrating that both increasing or decreasing signaling at these receptors prevents the expression of the conditioned motivational response. Furthermore, I report that the induction of nicotine dependence increases CRF mRNA in VTA DA neurons, and that blocking either the upregulation of CRF mRNA or the activation of VTA CRF receptors prevents the anxiogenic and aversive motivational responses to withdrawal from chronic nicotine. The results described in this thesis provide novel evidence of a VTA DA/CRF system, and demonstrate that both CRF and a specific pattern of tonic DA activity in the VTA are necessary for the aversive motivational experience of nicotine withdrawal.

Keywords: nicotine
dopamine
withdrawal
conditioned place aversion
opponent process theory of motivation
corticotropin-releasing factor
motivation
D1 receptor
D2 receptor
neuropharmacology
phasic dopamine
drug addiction
tonic dopamine
ventral tegmental area
Advisor: Kooy, Derek van der
Cast Steel Yielding Brace System for Concentrically Braced Frames

This thesis presents the development and validation of a high ductility seismic resistant steel brace connector called the Yielding Brace System (YBS) that improves the earthquake performance of steel braced frame buildings. The connector is comprised of two steel castings which dissipate seismic energy through flexural yielding of specially designed triangular yielding fingers. In this body of work, the need for such a system is presented along with a summary of previously developed steel castings for enhanced earthquake performance of building structures. The development of the YBS concept is then discussed in detail and equations are developed to predict the elastic and plastic response of a YBS connector based on the geometry of the yielding fingers. The low-cycle fatigue life of the cast steel material used for the yielding elements of the YBS is characterized based on the results of several cyclic, small-scale yielding fingers tests and a low-cycle fatigue life prediction model is derived. Following this, the design of a prototype connector for the second storey brace of a fictitious six storey sample building located in Los Angeles is presented. This design is conducted using the low-cycle fatigue prediction model, the response prediction equations and non-linear finite element analysis. Results of four full-scale prototype tests are then presented. Two of the tests are axial tests of the device alone, while the other two are full-scale braced frame tests. Finally, the design of a 12-storey sample building is presented. This building design is then evaluated via non-linear time-history analysis using the FEMA P-695 methodology. The results from these analyses are then discussed and compared to a similar study conducted on the same building designed with buckling restrained braces. This work shows that the Yielding Brace System is a highly ductile, seismic resistant brace that can be used as an alternative to the buckling restrained brace with the potential to provide a stiffer structure with increased ductility.

Keywords: Cast Steel
Bracing
Seismic
Yielding
Steel Structures
Advisor: Christopoulos, Constantin
Packer, Jeffrey A.
MicroRNAs as Prognostic Biomarkers in Prostate Cancer

Prostate cancer, one of the most common cancers among men, can be relatively harmless or extremely aggressive. The most widely used biomarker for the disease, the PSA test, is not independently diagnostic or prognostic of prostate cancer. One of the main challenges of prostate cancer research is to find reliable and effective prognostic biomarkers that will predict cancer recurrence following surgery, in order to identify clinically significant prostate cancer and improve management of the disease. In recent years, microRNAs (miRNAs) have been identified as master regulators of cellular processes, and dysregulated miRNAs have been associated with cancer development and progression. The intent of my PhD research program was to uncover novel miRNAs that contribute to prostate cancer pathogenesis in order to assess their potential as predictors of clinical progression. By analyzing a large cohort of primary prostate cancer samples, we have discovered that microRNA-221 (miR-221) is associated with metastasis and biochemical recurrence in prostate cancer, and is downregulated in TMPRSS2:ERG fusion gene- positive tumors. In addition, we have determined that microRNA-182 (miR-182) is overexpressed in prostate cancer and is associated with increased metastasis and clinical progression by targeting a tumors suppressor Forkhead box O1 (FOXO1). Overall, this work introduces novel candidate miRNA genes and downstream targets that are aberrantly expressed in more aggressive prostate cancer, and presents a potentially significant role for miRNAs as prognostic biomarkers that are associated with clinical progression, and perhaps aids in defining how miRNAs might one day serve as anti-cancer therapeutic agents.

Keywords: prostate cancer
MicroRNA
Advisor: Seth, Arun
The Practice of Doctoral Education: A Bourdieusian Analysis of the Socialization of Doctoral Students

Attention to doctoral education from scholars and policy makers has increased dramatically over the last two decades. Recent research on doctoral education has focused on the experiences of doctoral student and on issues related to financial aid, time to degree, completion rates, supervisor relations and socialization. The socialization framework has been used most frequently to understand the experiences of doctoral students, and this research continues to explore students’ experiences through the lens of socialization. A crucial component of this research is the use of Pierre Bourdieu’s theory of practice to examine doctoral education, in general, and the socialization of doctoral students, in particular. The concepts of habitus, capital, field and practice were used to explore doctoral education as constitutive of particular processes and expectations that underscore specific notions of success. In doing so, the socialization of doctoral students was examined through these tools to determine the extent to which different socialization mechanisms were experienced differently by students. Finally, an investigation into the histories, experiences and relationships of these students prior to enrolment in doctoral study suggested that particular elements of students’ pasts were highlighted as significant factors to their understanding of the expectations of doctoral study and ability to demonstrate competence with academic tasks, both of which impacted their socialization during doctoral education. This research revealed that doctoral education operated with particular rules and expectations that promote specific notions of success. These rules, expectations and parameters of success were deeply tied to demonstrations of task competence through the traditional academic tripartite. Considerable discussion highlighted operationalizations of “research” that included securing external, competitive scholarships, publishing in academic, peer-reviewed venues and presenting at disciplinary academic conferences. Students who were able to achieve these experiences were deemed to be more “successful” during doctoral study. Importantly, there was a tendency of “reinforcing advantage” to the experiences of “successful” doctoral students to the extent that those students who demonstrated acumen with particular aspects of academic work were offered and encouraged to take on more experiences and responsibilities that enriched their doctoral education.

Keywords: Doctoral Education
Socialization
Bourdieu
Advisor: Jones, Glen A.
Identification of Heat Shock Factor Binding Sites in the Drosophila Genome

The heat shock response (HSR) is a highly conserved mechanism that enables organisms to survive environmental and pathophysiological stress. In Drosophila, the HSR is regulated by a single transcription factor, heat shock factor (HSF). During stress, HSF trimerizes and binds to over 200 loci on Drosophila polytene chromosomes with only nine mapping to major heat shock (HS) inducible gene loci. The function of HSF binding to the other sites in the genome is currently unknown. Some of these sites may contain yet unidentified “minor” HS genes. Interestingly, the binding of HSF also coincides with puff regression at some sites. Two such sites contain the major developmentally regulated genes Eip74 and Eip75: key regulators in the response to 20-hydroxyecdysone (20E), the main hormone responsible for the temporal co-ordination of post-embryonic development in Drosophila. Previous work in our and other labs indicates that the regression of non-HS puffs during the HSR is dependent on the presence of functional HSF. Using chromatin immunoprecipitation (ChIP) followed by hybridization to genome tiling arrays (Chip), I have identified 434 regions in the Drosophila Kc cell genome that are bound by HSF during HS, and have determined that 57% of these sites are located within the transcribed regions of genes. By examining the transcriptional response to HS in Kc cells and third instar larvae using expression microarrays, I found that only about 10% of all genes within 1250 bp of an HSF binding site are transcriptionally regulated by HS and many genes whose transcript levels change during HS do not appear to be near an HSF binding site. Furthermore, genes with an HSF binding site within their introns are significantly enriched (modified Fisher Exact p-value between 2.0x10-3 and 1.5x10-6) in gene ontology terms related to developmental processes and reproduction. Using expression microarray technology, I characterized the transcriptional response to 20E and its structural analog ponasterone A. I have identified multiple HSF binding sites within Eip74 and Eip75, and show that induction of the HSR correlates with repression of these genes and all other 20E-inducible genes. Taken together, this work provides a basis for further investigation into the role of HSF binding to sites not associated with HS genes and its possible function as a repressor of gene transcription during conditions of stress and as a regulator of developmental genes under stress and non-stress conditions.

Keywords: HSF
Heat Shock Factor
Heat Shock Response
transcription
gene expression
genomics
ChIP-on-chip
ecdysone
Eip75B
Ponasterone A
20-hydroxyecdysone
ecdysone response
hsf4
transcription factor
models of transcription
genome tiling arrays
Heat Shock Protein
Heat Shock Gene
Advisor: Westwood, J. Timothy
Characterization and Synthesis of Cyclodextrin Inclusion Complexes and their Applications as Fluorescent Probes for Sensing Biomacromolecules

Cyclodextrins (CDs) are macrocycles composed of several glucose units bound through α-1,4 glycosidic linkages. They can be chemically modified to display functional groups on their primary or secondary rim. CDs display these groups in defined geometries ideally suited to bind biomacromolecules. Moreover, CDs have a hydrophobic cavity that allows them to form stable host-guest complexes with lipophilic molecules. This combination of functionality and guest binding ability makes CDs important scaffolds for the design of functional supramolecular systems. This thesis explored the interaction of heptakis-[6-deoxy-6-(2-aminoethylsulfanyl)]-β-cyclodextrin (1) with many hydrophobic guest molecules. The binding constants of CD host-guest interactions were measured using ITC and fluorometry-based approaches. These studies revealed 1 to form the highest affinity 1:1 cyclodextrin-guest complexes reported to date. This thesis then explored the use of CD inclusion complexes as biomacromolecular sensors. CD 1 and its derivatives were used to develop self-assembling sensors. First, a library of polycationic CDs with differing charge distribution was synthesized. The sensing motif was synthesized by covalently linking a quinolinium fluorophore to lithocholic acid (LCA). The CD-based binding motifs and the LCA-based sensing motif self-assemble through host-guest interactions (i.e. 1 binding to LCA displays a Ka = 5.52 × 107 M-1). These inclusion complexes were then used as an array of self-assembling sensors capable of differentiating between pure and contaminated samples of heparin (anticoagulant). To capitalize on the promise of CD 1 a new technique was explored to functionalize a single amine of 1. The technique relies on an S to N acyl transfer from a guest molecule to a CD host resulting in the mono-acylation of the host. The importance of the linker between the guest and the reactive acylating agent was fully explored. Furthermore, two CD probes are synthesized and are shown to display differential fluorescent responses with a small series of proteins.

Keywords: Cyclodextrin
Chemistry
Advisor: Nitz, Mark