VictorJuiz/thesis huggingface dataset:
As We Move Ahead Together: Foregrounding Reconciliating and Renewed First Nation/ Non-Aboriginal Relations in Environmental Management and Research

The research project upon which this dissertation is based focused on enhancing understandings of the nature of current First Nations/non-Aboriginal relations in environmental management. The project was undertaken as a collaborative initiative by the author, a non-Aboriginal doctoral researcher, in partnership with Walpole Island First Nation. The research served as an opportunity for co-producing knowledge on this subject across cultures and worldviews, and as an effort to build towards our shared aspiration of learning how distinct, yet inextricably linked, First Nations/non-Aboriginal understandings, approaches and worldviews can come together within a context of mutual respect and mutual benefit. The purpose of the research was to investigate the existence and types of issues leading to First Nations/non-Aboriginal tensions in environmental management, to analyze and unpack underlying causes of challenges identified via the research, and to construct avenues for relationship improvement. The research project was grounded in a specific investigation into relations in species at risk conservation and recovery in southern Ontario, Canada. The resulting dissertation is structured around three primary focal areas: 1) investigating and exposing colonial influences at play in Canada’s Species at Risk Act, and offering a new model for co-governance in this arena and beyond; 2) investigating relations surrounding efforts towards traditional ecological knowledge (TEK) transfer in species at risk work, with a focus on exploring issues identified in relation to intellectual imperialism; and 3) introducing and characterizing an original, reconceptualized approach to First Nations/non-Aboriginal relationships in academic research; this approach focused on ways in which investigatory practice can become a means of working towards broader reconciliation goals. Research findings from this dissertation indicate that colonial factors, often unevenly visible to actors involved in environmental management and research, continue to strongly affect the potential for positive, productive First Nations/non-Aboriginal relations in these spheres - including within the species at risk conservation and recovery arena examined here. Project results provide insight into the nature of the factors influencing relationships, as well as potential avenues for addressing the vitality of colonialism in contemporary relations and overcoming the influences on First Nations and on First Nations/non-Aboriginal relationships.

Keywords: Indigenous
Species at risk
Indigenous knowledge
First Nations/non-Aboriginal relations
Environmental management
Endangered species
Endangered ecosystems
Community-based research
Decolonization
Reconciliation
Decolonizing research
Traditional ecological knowledge
Southern Ontario
Collaborative research
Aboriginal
First Nation
Colonialism
Species at Risk Act
Governance
Intellectual imperialism
Advisor: McGregor, Deborah
Psychosocial Responses to Falling in Older Chinese Immigrants Living in the Community

Background and Rationale: Falls are among the most common problems faced by elderly persons. While the physical risk factors for falls are well established, the psychosocial aspects have been largely neglected. Moreover, studies exploring the responses to falls from the individuals’ perspectives in an immigrant population are virtually non-existent. The older Chinese immigrant population is substantial and growing in the Greater Toronto Area. The cultural and immigrant-related factors that influence their responses to and recovery after a fall are important considerations as health and social care professionals develop falls prevention strategies, and provide services and care. This dissertation explored the nature of immediate and subsequent responses of community-dwelling older Chinese immigrants after falling. Method: Focussed Ethnography, as guided by elements from Critical Social Theory, was used as the research methodology. Eighteen informants over 70 years of age, living in the community who had experienced a recent fall were interviewed using a semi-structured guide developed after a detailed literature review. Thematic analysis of transcribed interviews was conducted. Results: Four major themes related to responses to falling were drawn forth from the interview data: 1) Help-seeking decisions immediately after the fall, 2) Psychological impact of the fall, 3) Care and support networks, and 4) Learning from the fall. Discussion: The psychosocial responses supported a “blended” explanatory model of illness. Respondents appeared to adhere to both Western medical models and traditional Chinese explanatory model depending on the severity of the fall injuries. Also, the roots of Chinese culture in the blended traditions and philosophies of Confucianism, Taoism, and Buddhism appear to be the foundation for many of the beliefs and attitudes expressed in this study, and these beliefs and attitudes in turn influence how Chinese fallers experience falling. The wide range of psychosocial responses also illustrated the complexity of the Andersen’s Behavioural model for health services use and its potential to explain the different types of services an older Chinese immigrant may need post-fall. Conclusions: Findings from this study provided key, previously unexplored insights into the cultural and immigrant-related factors that influence the psychosocial experience, vulnerability and care-seeking behaviours of older Chinese immigrants following a fall.

Keywords: Psychosocial
Fall
Chinese immigrants
Community-dwelling
Advisor: Sadavoy, Joel
Toward Photo-control of Peptide Structure in Vivo

An ability to manipulate the activity of a specific protein inside living cells offers exciting prospects for the study of protein function in vivo. Azobenzene derivatives introduced as intramolecular bridges have been demonstrated to reversibly photoregulate secondary structures and functions of peptides and proteins in vitro. My overall goal is to create a generally-applicable process for the reversible photocontrol of protein-protein interactions within the complex environment of a living cell. Results of studies toward this aim are presented. A blue-green absorbing (~480 nm) azobenzene derivative cross-linker was designed that reversibly controlled the helical content of attached peptides with a half-life of the cis state of ~50 ms. This rapid photoswitch may prove useful as a tool for probing dynamic processes in biochemical systems using light. The effect of cross-linker position (N-terminus, middle, C-terminus) on a photo-switchable 32-residue helical peptide was studied. Although the activation energies for thermal cis – trans relaxations were not the same, linker position did not affect the change in helix content. This work provides useful information for the effective photoregulation of much longer helices such as occur in coiled-coils. Fluorescently labeled, cross-linked, modified Fos/Jun peptides with and without cell-penetrating peptide (CPP) tags were prepared for the purpose of photocontrolling peptide-peptide interactions in vivo. One of the peptides showed a degree of photocontrol of helicity. Cell uptake of CPP-tagged peptides was demonstrated. However, overall peptide behavior was dominated by undesired aggregation. A simple reporter, a cross-linked peptide bearing an environmentally sensitive fluorophore at a key site, was designed for detecting photoswitching in vivo. Photoisomerization of the cross-linker caused changes in the local chemical environment and changes in fluorescence intensity of the environmentally sensitive dyes in vitro. However, no change in fluorescence was observed in the living systems we investigated. Conclusions and suggestions for further work aimed at achieving the overall goal stated above are discussed.

Keywords: Azobenzene
Cross-linker
Photo-control
in Vivo
Advisor: Woolley, Andrew
“Continuing a normal life as a normal person”: A Hermeneutic Phenomenological Study on the Reconstruction of Self Identity of Chinese Women Within the Lived Experience of Breast Cancer Survivorship

Breast cancer incidence in Chinese women is rising in North America. However, a critical review of the empirical research reveals a clear under-representation of the breast cancer survivorship experiences of ethnic minority women, particularly those of Chinese origin. A breast cancer diagnosis not only disrupts a woman’s everyday life but also, and more importantly, her self-identity: who she was before the cancer diagnosis and who she becomes after the diagnosis. The purpose of this study was to understand the lived survivorship experience of Chinese women with breast cancer, in particular the way they reconstruct their self-identity while living under the constant threat of premature mortality. A hermeneutic phenomenology was employed to illuminate the essence of the lived experience. A purposeful sample of 24 Chinese women was recruited, and audiotaped face-to-face semi-structured interviews were conducted in English or Mandarin. An iterative process was carried out to identify themes and interwoven them into the four existentials of lifeworld to lend structural meaning to the lived experience. The self-identity of Chinese women living with breast cancer did not fit the current combative survivor identity and narrative as represented in the North America media. Rather, a ‘quiet’, modest and practical narrative underscoring the Chinese virtues of self-reliance, endurance, and social responsibility and harmony characterized their lived experience and self-identity. They endured unexpected major life events and accepted what life offers in an effort to move on with their lives of being a normal person again. An understanding of the way Chinese women manage the impact of breast cancer and their survivorship experience will significantly contribute to building our knowledge about this minority population within the Canadian context. In turn, this understanding will support health care professionals with the development of culturally sensitive psychosocial/supportive care services to maximize adaptation and recovery.

Keywords: breast cancer
Chinese women
culture
survivor
identity
survivorship experience
phenomenology
translation
Advisor: Williams, Charmaine
Molecular Mechanisms of the Cooperation between Rac1/1b GTPases and the Canonical Wnt Signaling Pathway in Colorectal Cancer

Aberrant activation of the canonical Wnt signaling pathway accounts for the vast majority of colorectal cancers. The Rac1 GTPase is overexpressed in colon cancer, and its splice variant, Rac1b, is preferentially expressed in colon tumours. Rac1 and Rac1b have both been previously shown to crosstalk with the canonical Wnt signaling pathway in colon cancer; however, the specific means by which this crosstalk occurs were unclear. This study examines the molecular mechanisms of Rac1/1b in the cooperation with canonical Wnt signaling in colon cancer. In a colon cancer cell line with dysregulated Wnt signaling, the constitutively active Rac1 mutant, V12Rac1, was observed to transcriptionally upregulate the expression of a gene set associated with cellular migration. Further, V12Rac1-mediated promotion of cell migration was dependent on its nuclear localization. Previous work in our lab has shown a Rac1-specific activator, Tiam1, is present in the nucleus at the promoter of Wnt target genes upon Wnt3a stimulation; and that exogenous introduction of Tiam1 increased the expression of a Wnt-responsive reporter (TopFlash). Given the importance of nuclear localization of Rac1 in the promotion of tumourigenic processes, we demonstrated that knockdown of endogenous Tiam1 reduced TopFlash expression, proving reverse specificity and strengthening the evidence of a nuclear role for Rac1. Since some functional differences exist between Rac1 and Rac1b, we also examined Rac1b for transcriptional targets following induction, and identified the RhoA effector, ROCK2, which has been previously associated with cell migration. ROCK2 demonstrated a positive correlation with Rac1b transcript expression in primary colon tumours as compared to matched normal tissue specimens. Interestingly, the observed induction in ROCK2 transcript did not translate into a detectable change in protein expression or kinase activity. Like Rac1, Rac1b also promotes cellular motility, which is dependent on nuclear localization. Cell migration can be negatively regulated by E-cadherin. Following Rac1b knockdown in HT29 cells, we show that Rac1b might contribute to motility through upregulation of the E-cadherin-repressor, Slug. Taken together, we provide greater insight into the mechanistic roles of Rac1 and Rac1b in transcriptionally regulating target genes to promote cellular processes, such as cell migration, in colon cancer with dysregulated canonical Wnt signaling.

Keywords: Rac1
Wnt
Colon cancer
Rac1b
Advisor: Bapat, Bharati
Is Hearing Believing? Perception of Online Information Credibility by Screen Reader Users who are Blind or Visually Impaired

While credibility perception on the Web is a well-researched topic across multiple disciplines, extant studies have not considered nonvisual modalities of Web access. This research explores how Web users who are blind or visually impaired perceive the credibility of online information and how the screen reader used by them to interact with the Web mediates the process. Credibility perception was studied in the context of the screen reader users’ everyday information practices, examining in depth the effect of Web accessibility on their online information interactions, information practices and credibility perception. Adopting an exploratory approach, a sequential multimethods research design was used. Between April and July 2008 data were collected from adult screen reader users residing in Ontario, Canada through an electronic questionnaire survey (N=60) to identify salient issues, which were then examined deeper through semi-structured interviews with a subsample (N=13) during June 2009. Hands-on online information activities (with participant observation and think-aloud protocol) were also conducted during the interview session. Primary findings emerged through qualitative content analysis of descriptive data, with quantitative results guiding and supplementing the analysis. Online information credibility perception is found to be a dynamic and social process. It is governed by users’ assumptions based on their past experiences, personal knowledge/beliefs and social inputs. Assumptions evolve over time and usage into personal heuristics. The credibility perception process spans three phases—prediction, evaluation and corroboration—permeating the information seeking, using and sharing practices of users. Evaluation of website and web content depends on users’ online interaction proficiency and is bounded by the interface affordances provided by the screen reader and the amount of meta-information provided by the websites for interpreting visual/spatial features. Community support scaffolds users towards more effective technology management and credibility perception. Therefore, promoting inclusion in the online participatory culture will enhance the information practices of screen reader users.

Keywords: Credibility perception
Blind or visually impaired
Web accessibility
Everyday information practices
Online participation
Advisor: Caidi, Nadia
Hockema, Stephen
What are you in the dark? The Transformatiive Powers of Manitouminasuc upon the Identities of Anishinabeg in the Ontario Child Welfare System

The purpose of this qualitative study is to explore, describe and examine how the child welfare experience affects the personal and social identities of Anishinabe participants. Contextual realities, specifically the cultural and child welfare contexts, and how participants situate themselves in those realities, are explored. A Critical Ekweism conceptual framework is used to inform the design of the study and analyze the unique experiences of participants. The framework seeks to respect and understand the unique historical backgrounds and perspectives of participants as they critically evaluate their contexts as knowers and experts of their own experiences. While participants collectively identified existing dilemmas and practices, they also decided to actively think of ways to re-address and to positively transform these dilemmas and practices. Methods of inquiry included the Aboriginal Circle paradigm that is interwoven with Phenomenological procedures. The Aboriginal Medicine Wheel was used as an organizational tool to illustrate and explain study findings, and Phenomenological procedures were used to explore the meanings participants append to their experiences. Both sharing circles and individual interviews were used to collect data from twenty-seven participants who were involved in the child welfare system at the time of the study. Some were living in Northern Ontario, others in a large city. Data were transcribed and Grounded Theory coding procedures used to analyze the data and identify themes. Four main themes emerged: Place of Understanding’, ‘Place of Disconnection’, ‘Place of Identification,’ and ‘Place of Reconnection’ to represent the sacred knowledge-making spaces where participants through the Reality Circle make sense of their contexts. The meanings that underpin each of these sacred spaces are discussed. An analysis of the meanings of these four sacred spaces further describes how participants’ personal and social identities are juxtaposed in their cultural and child welfare contexts. Of interest is how participants’ child welfare experiences affect their cultural and parenting identities. Child welfare practices are interpreted in terms of parent, social work and First Nations Community responsibilities. A diagram depicting these responsibilities is presented as the ‘Anishinabe Identity Circle.’ The study is significant for the social work profession because an Anishinabe approach to ‘doing’ social work with this particular group of participants is developed and has implications for Aboriginal-based Theory and Aboriginal-based support and policies. Even though this is a small step towards changing some of the existing practices in the Child Welfare System, it may pave the way for larger and more constructive social changes for participants and their children in the future.

Keywords: Aboriginal Identity
Child Welfare
Relationships
Social Change
Philosophy
Social Policy
Personal Development
Culture
Feminism
Advisor: Neysmith, Sheila
The Construction of Self-identity and Positive Behavioural Change in Pregnant and Parenting Young Women

The purpose of this mixed method study was to investigate the relationship between the narrative construction of self-identity and positive change in antisocial behaviour in pregnant and parenting young women. It focused on two related aspects of identity development: (1) individuals’ conceptualizations of their personally salient self-values; and (2) “self-action coherence”: the process of constructing self-narratives that establish coherence between one’s personally salient self-values and behaviour. This study also included a qualitative exploration of how becoming a mother in adolescence and early adulthood is related to processes of identity development and behavioural change. Participants were 27 pregnant and parenting young women (ages 16 to 22) recruited from youth-serving agencies in Toronto, Ontario. Participants completed a questionnaire on history of engagement in antisocial behaviour and a semi-structured interview that explored self-identity and critical life experiences. Analyses of participant interviews suggest that positive behavioural change in pregnant and parenting young women is related to active engagement in self-reflection motivated by a convergence of meaning gleaned from a variety of life experiences, including the transition to motherhood. Quantitative findings suggest that: (1) an orientation to relational values is related to lower reported recent engagement in antisocial behaviour; (2) self-action coherence develops across adolescence and early adulthood; and (3) self-action coherence is related to reported positive behavioural change. Overall, the findings suggest that an orientation to relationships is important for establishing positive patterns of behaviour and that positive behavioural change in pregnant and parenting young women involves a process of constructing personally salient self-values and establishing behaviours that cohere with these values. The findings have theoretical implications relating to identity development in adolescence and early adulthood and its relations to behavioural functioning. The findings also have implications for applied work with pregnant and parenting young women with histories of antisocial behaviour.

Keywords: identity
narrative
positive youth development
early motherhood
Advisor: Arnold, Mary Louise
Magnetic Resonance Imaging to Identify Intraplaque Hemorrhage and Define its Location in Complicated Carotid Artery Plaques

Atherosclerotic plaque (AP) composition is an important factor influencing plaque rupture. Intraplaque hemorrhage (IPH) is a marker of complicated-plaque formation, responsible for many of the clinical manifestations of atherosclerotic disease. Magnetic resonance imaging (MRI) has emerged as a modality to image carotid AP. The in-vivo high-resolution MR imaging of carotid complicated-plaque forms the basis of this thesis. In the first part, Magnetic Resonance Imaging of Intraplaque Hemorrhage (MRIPH), an in-vivo high-spatial-resolution 3-dimensional MRI sequence specifically designed to depict complicated-plaque in the carotid arteries is described. MRIPH was applied, as compared with histologic analysis (gold standard), to demonstrate that T1-hyperintense intraplaque signal represented blood products. Strong agreement was seen between T1-hyperintensity and histologically-identified hemorrhage, with high sensitivity/specificity/positive- and negative-predictive values for T1-hyperintense detection of hemorrhage. While IPH increases plaque rupture risk, high degrees of calcification promote stability. Calcium can generate T1-hyperintensity in some gradient-echo (GRE) sequences. Therefore, distinction between these two components is crucial. In the second part, T1-hyperintensity in MRIPH was shown to be almost exclusively due to hemorrhage and not calcification by directly comparing in-vivo T1-hyperintensity with calcification in ex-vivo specimens imaged with microCT. T1- hyperintesity showed very good albeit inverse agreement with calcification and excellent agreement with lack of calcification as seen on microCT. IPH is thought to be the result of rupture/leakage of the vasa vasora. In the third part, we tested the hypothesis that if IPH were due to vasa vasorum rupture/leakage, the majority of the IPH would be associated with the adventitial rather than the luminal surface of the plaque. Deep (closer to vessel wall) and superficial (closer to vessel lumen) regions of complicated plaques were identified. Very good inter-rater agreement was seen for the location of IPH using MRIPH, with IPH being more frequently present in the deeper compared to superficial segments of the plaque. In summary, an in-vivo MR technique to detect IPH at high spatial-resolution in carotid complicated-plaque was developed; demonstrating T1-hyperintensity in MRIPH is the result of IPH and not calcification. The predilection of IPH for the deeper segments of the plaques suggests that IPH is due to vasa vasorum rupture/leakage.

Keywords: Intraplaque hemorrhage
Magnetic Resonance Imaging
Advisor: Moody, Alan