VictorJuiz/thesis huggingface dataset:
Encounters with the Real: A Zizekian Approach to the Sublime and the Fantastic in Contemporary Drama

This study brings the insights of Slavoj Žižek’s Lacan-inspired approach to bear upon a series of influential 20th century plays and their engagement with what Lacan calls the Real. The plays to be explored share a focus on experiences, events or encounters which transcend, exceed, disrupt, and in some cases shatter characters’ normal, familiar realities. Examined through the lens of Žižek, these confrontations with the sublime and the fantastic reveal a crucial relation to the plays’ contemporary contexts, prompting us to “look awry” upon the dynamics of our own symbolically-regulated reality and the ever-changing and precarious nature of our relation to it. Similarly crucial is the relation of the Lacanian Real to our theatrical forms and modes of perception in the theatre. In staging “encounters with the Real,” these plays prompt us simultaneously to explore the ways in which the Real operates —and “appears” — in our own theatrical experience, ensnaring our gaze and the force of our desire. The study offers a Žižekian approach to works including Peter Shaffer’s Equus, John Mighton’s Possible Worlds, S. An-sky’s The Dybbuk, or Between Two Worlds, Caryl Churchill’s The Skriker, Tony Kushner’s The Illusion, and Eric-Emmanuel Schmitt’s Enigma Variations.

Keywords: drama
Slavoj Zizek
theatre
psychoanalysis
Caryl Churchill
Peter Shaffer
S. An-sky
Tony Kushner
Jacques Lacan
John Mighton
Eric-Emmanuel Schmitt
The Dybbuk
Equus
The Skriker
Possible Worlds
The Illusion
Enigma Variations
sublime
fantastic
Advisor: Barton, Bruce
Structural and Functional Characterization of Leukocyte-type Core 2 {beta}1,6-N-acetylglucosaminyltransferase

Leukocyte type core 2 {beta}1,6-N-acetylglucosaminyltransferase (C2GnT-L) is a key enzyme in the biosynthesis of branched O-glycans. It is an inverting, metal ion-independent glycosyltransferase that catalyzes the formation of the core 2 O-glycan (Gal{beta}1,3[GlcNAc{beta}1,6]GalNAc-O-Ser/Thr) from its donor and acceptor substrates, UDP-GlcNAc and the core 1 O-glycan (Gal{beta}1,3GalNAc-O-Ser/Thr), respectively. The primary objective of the work described in this thesis is to shed light on the structure, catalytic mechanism and substrate specificity of C2GnT-L. Since glycosyltransferases are membrane bound glycoproteins that possess disulphide bonds, the first challenge was to produce sufficient quantities of C2GnT-L for biochemical and structural characterization. To this end, C2GnT-L and various active site mutants were expressed and purified from stably transformed mammalian cell lines. The x-ray crystal structure of wild-type C2GnT-L was solved, in both its apo and acceptor substrate complexed forms. The structures, along with revealing the structural basis for acceptor substrate specificity, showed that C2GnT-L belongs to the GT-A glycosyltransferase fold type. This was a surprising result given that, to this day, C2GnT-L is the only GT-A glycosyltransferase that does not possess a DXD motif and does not require a divalent metal ion for catalysis. The mechanism of the metal ion-independent C2GnT-L activity, within the context of the metal ion-dependent GT-A fold, was probed further using site directed mutagenesis in conjuncition with x-ray crystallography, enzyme assays, and frontal affinity chromatography. It was found that positively charged side chains in C2GnT-L functionally replace the divalent metal ion found in other GT-A glycosyltransferases, providing evidence for a convergence of metal ion-independent activity between GT-A and GT-B glycosyltransferase fold types.

Keywords: glycosyltransferase
crystallography
C2GnT
Advisor: Rini, James
Cardiovascular, Utero- and Fetoplacental Function in Mice during Normal Pregnancy and in the Absence of Endothelial Nitric Oxide Synthase (eNOS)

In pregnancy, the maternal cardiovascular and placental circulation undergoes structural and functional changes to accommodate the growing fetus, but the mechanisms involved are not fully understood. Nitric oxide (NO) increases in normal pregnancy and lack of NO has been implicated in pregnancy related complications, preeclampsia and fetal growth restriction. Thus, the objective of the thesis was to determine if cardiovascular, uteroplacental and fetoplacental changes observed in human pregnancy also occur in mice and to assess the obligatory role of eNOS in mediating these changes. I showed that like humans, mice exhibit increases in maternal cardiac output, stroke volume, plasma volume, and uterine arterial blood flow, and a transient decrease in arterial pressure during pregnancy. Importantly, I showed that endothelial nitric oxide synthase (eNOS) plays an important role in promoting the progressive increase in maternal cardiac chamber dimensions and output and the enlargement of the aorta during pregnancy in mice. Another novel finding was that eNOS plays an important role in remodeling of the uterine and umbilical vasculatures during pregnancy. The remodeling of the uterine vasculatures, including the uterine and spiral arteries, were blunted in the eNOS KO mice with ko fetuses (KO(ko)) and this likely contributed to elevated vascular resistance and reduced perfusion of the uterine circulation during pregnancy. Impaired spiral artery remodeling may be caused by a deficiency in decidual uterine natural killer cells. Fetal placental vascularization was also impaired in eNOS KO(ko) mice, which likely increased vascular resistance and thereby reduced fetoplacental perfusion. Reduced vascularization may be due to decreased VEGF mRNA and protein expression in KO(ko) placentas. Decreased perfusion in both the uterine and umbilical circulations most likely contributed to elevated placental and fetal hypoxia in the eNOS KO(ko) mice. Interestingly, despite placental hypoxia, eNOS KO(ko) mice do not show the classical signs of preeclampsia including hypertension and proteinuria nor are maternal plasma sFlt1 levels elevated. Nevertheless, eNOS KO(ko) pups are growth restricted at term, and this is mainly due to the fetal genotype. These findings suggest that eNOS plays an essential role during pregnancy in remodeling of the maternal heart, aorta, and uterine and umbilical vasculatures thereby augmenting blood flow to the maternal and fetal sides of the placenta and thereby promoting fetal growth in mice.

Keywords: eNOS
Pregnancy
cardiovascular
uterine circulation
umbilical circulation
ultrasound
preeclampsia
IUGR
mouse
remodeling
placenta
VEGF
Advisor: Adamson, Susan Lee
Functional Magnetic Resonance - and Diffusion Tensor Imaging Investigations of Pure Adult Gilles de la Tourette Syndrome

Gilles de la Tourette syndrome (GTS) is a chronic neuropsychiatric disorder characterized by multiple motor and vocal tics, affecting approximately 1% of the population. The precise neuropathology of GTS has not yet been delineated, but current models implicate subcortical and cortical areas - the cortico-striato-thalamo-cortical (CSTC) circuit. The majority of studies in the literature have either dealt with GTS with comorbid conditions and/or children with GTS. As these factors are known to affect brain structure and function, it unknown what the neurobiological underpinnings of pure adult GTS are. The objective of this body of work was to use functional magnetic resonance imaging (fMRI) and diffusion tensor imaging (DTI) to characterize differences in brain function and structure in pure adult GTS patients versus age- and sex-matched controls. I employed a series of three distinct analyses for this purpose, based upon current models of CSTC circuit-related dysfunction in GTS. In the first, GTS patients and control participants executed three finger-tapping paradigms that varied in both complexity and memory requirements. These finger-tapping tasks were modeled after previous studies that showed CSTC circuit-related activity in healthy individuals. Using a multivariate statistical technique to assess task-related patterns of activation across the whole brain, I found that, while there was much overlap in brain activation patterns between groups, sensorimotor cortical regions were differentially recruited by GTS patients compared to controls. In the second fMRI analysis, I measured low-frequency spontaneous fluctuations of the blood oxygen level dependent signal during rest, and found that GTS patients exhibited greater resting state functional connectivity with the left putamen compared to controls. In the final analysis, DTI was used to provide a whole-brain assessment of regional diffusion anisotropy in GTS patients and healthy volunteers and to investigate the fractional anisotropy in predetermined ROIs. This analysis found no differences between GTS patients and controls. Overall, my findings indicated that several CSTC-related regions shown to be atypical in GTS patients previously, are also atypical in pure adult GTS, and that sensorimotor cortical regions and the putamen may be regions of functional disturbance in pure adult GTS.

Keywords: Neuroimaging, fMRI, DTI, Tourette Syndrome, Movement Disorders, Multivariate Statistics
Advisor: Sandor, Paul
Cdc42 and Par Proteins Regulate the Trafficking of Apical Membrane Proteins to Stabilize Dynamic Adherens Junctions in the Drosophila Neuroectoderm

Epithelial sheets line the surfaces of the body, forming a barrier between the external environment and internal tissues. During development, regulation of epithelial architechture can drive morphogenesis and build the three-dimensional structures of the body. Epithelial form and function derive from the polarized morphology of epithelial cells, which have apical surfaces that face the external environment, lateral surfaces containing cell-cell junctions and basal surfaces that connect to the underlying tissue. A network of polarity proteins establishes the apico-basolateral axis, while a system of polarized membrane traffic ensures delivery of specialized cargo to distinct membrane surfaces. How these systems of polarity and trafficking are integrated is still poorly understood. The focus of my study was to investigate how the apical polarity proteins Cdc42, Par6, Bazooka and aPKC (the “Par complex”) regulate polarity and adherens junction (AJ) integrity during Drosophila development. Upon perturbation of Cdc42/Par activity during embryogenesis, apical membrane proteins accumulate in sorting endosomes. This trafficking defect occurs throughout the ectoderm, but in the ventral neuroectoderm (VNE) is accompanied by a concomitant depletion of the apical proteins from the plasma membrane (PM) and a loss of AJ integrity. I have demonstrated that the VNE phenotype is a consequence of the relatively high morphogenetic activity of this tissue. Furthermore, I have shown that the AJ defects are likely a downstream consequence of the depletion of important apical polarity factors, such as Crumbs, from the PM. To further characterize the mechanism of apical trafficking, I searched for interactors of Cdc42/Par in the membrane trafficking machinery. I describe interactions between several trafficking genes and Cdc42/Par and provide evidence that Vps26, a component of the retromer complex that retrieves proteins from endosomal membrane and delivers them to the Golgi for re-secretion, is phosphorylated by aPKC and acts as an aPKC effector in the recycling of apical membrane proteins. I propose that Cdc42/Par regulate the retromer to promote the PM localization of apical proteins, which is important to maintain AJ integrity in morphogenetically active tissues.

Keywords: Epithelial polarity
Cdc42
Advisor: Tepass, Ulrich
Sulphur Chemistry in KOH-SO2 Activation of Fluid Coke and Mercury Adsorption from Aqueous Solutions

The technical feasibility of producing sulphur-impregnated activated carbons (SIACs) from high-sulphur fluid coke by chemical activation was investigated. Using KOH and SO2, the activation process was able to produce SIACs with controllable specific surface area (SBET), pore size distribution and sulphur content. The highest SBET was over 2500 m2/g and the highest sulphur content was 8.1 wt%. K-edge X-ray Absorption Near Edge Structure (XANES) spectroscopy was employed to characterize the sulphur in fluid cokes and SIACs. The results revealed that the sulphur on fluid coke surface was mainly in the form of organic sulphide and thiophene (total 91-95 %), in addition to some sulphate (5 - 9%). The study of KOH-treated fluid coke suggested that KOH was effective in converting organic sulphide and thiophene to water soluble inorganic species which were readily removed by acid and water washing. SO2 treatment of fluid coke added sulphur to fluid coke through SO2-carbon reaction. Elemental sulphur was the main product, while part of the thiophene, sulphide and sulphate in the raw coke remained in the product. In KOH-SO2 activation, disulphide, sulphide, sulphonate and sulphate were identified on SIAC surface; no thiophene was found, suggesting a complete removal of thiophene. Sulphur content in specific forms in SIACs was therefore controllable by varying the ratio of KOH, SO2 and fluid coke. SIACs produced from KOH-SO2 activation showed a comparable Hg2+ adsorption capacity (43 – 72 mg/g) with those reported in the literature (35-100 mg/g) and that of a commercial SIAC (41 mg/g). Although a larger SBET often resulted in a greater Hg2+ adsorption capacity, the benefit started to diminish when SBET was greater than about 1000 m2/g. A statistically significant and positive correlation was found between Hg2+ adsorption capacity and total sulphur content. Elemental sulphur and reduced sulphur were largely responsible for the enhanced Hg2+ adsorption.

Keywords: Sulphur chemistry
Chemical activation
Sulphur-impregnated activated carbon
mercury adsorption
Advisor: Jia, Charles Q.
Rewriting the Social Contract: The Social Fund and Egypt's Politics of Retrenchment

The politics surrounding retrenchment and social protection in the Middle East have been obscured by a broad ideological consensus that civil society has replaced the state as the site of social provisioning since the nineties. Contrary to the dominant “state retreat” narrative, the adoption of neo-liberalism in the region was not in fact uniformly accompanied by convergence around a minimal welfare regime. Why have processes of welfare retrenchment unfolded along contrasting patterns across the Middle East with some states explicitly redefining social policy frameworks, and others undermining access and effects of prevailing programs without dismantling them? The dissertation aims to contribute to our understanding of state-society relations in the region by closely examining recent welfare regime changes in Egypt. Why has Egypt pursued “hidden retrenchment” entailing dilution of universal benefits, conversion of social programs to new beneficiaries and institutional layering, without the explicit overhaul of welfare policy frameworks? What are the micro-level political influences shaping the retrenchment process on the ground? Using the Social Fund for Development as a window for understanding hidden retrenchment in Egypt, the dissertation demonstrates that external dynamics of globalization, and donor assistance do not mainly account for welfare regime restructuring. Similarly, the state’s fiscal status, and the underlying switch in development strategies cannot explain retrenchment patterns. Rather, I argue that the internationally dominant neoliberal development discourse has influenced some aspects of retrenchment reforms, and domestic political dynamics have molded hidden retrenchment in Egypt. The regime’s power maintenance logic and a prevailing moral economy of social entitlements explain the process. Micro-level qualitative and statistical analyses of retrenchment politics also reveal that intra-state agencies struggles, regime security concerns, the state’s tendency to fiscally penalize areas with a history of Muslim Brotherhood support, as well as the National Democratic Party’s patronage networks influence outcomes on the ground. My findings suggest that variations in retrenchment patterns across the region reflect important differences in states’ social bases of power, rather than external pressures or domestic economic dynamics.

Keywords: Egypt
social policy
micro-finance
small business
Advisor: Sandbrook, Richard
Art and Politics of Appropriation

This thesis works towards a theory of creative appropriation as critical praxis. Defining ‘appropriation’ as the re-use of already-authored cultural matter, I investigate how the ubiquity of aesthetically and commercially motivated appropriative practices has impacted concepts of creativity, originality, authorship and ownership. Throughout this thesis, appropriation is understood as bridging the artistic, political, economic, and scientific realms. As such, it strongly affects cultural and socio-political landscapes, and has become an ideal vehicle for effectively criticizing and, perhaps, radically changing dominant aesthetic, legal and ethical discourses regarding the (re)production, ownership and circulation of knowledge, artifacts, skills, resources, and cultural matter in general. Critical appropriation is thus posited as a political strategy that can draw together the different causes motivating appropriative processes across the globe, and organize them for the benefit of a multitude which values concepts of reusing, sharing and collectivity over concepts of the individually authored and the privately owned. My arguments regarding this critical potentiality are based on concrete practices emanating from several media (textual – visual – sonic – digital). The corpus includes Berlin Dadaist collage, ‘found footage’ filmmaking, audio sampling, and digital media art. It is critically contextualized in the fields of philosophy, law, and aesthetics, and paired with relevant examples from extra-aesthetic arenas (economics, industrial production and science). Following a trajectory from the analog to the digital, my thesis traces the emergence and tactical employment of critical appropriative practices in the context of different historical, philosophical, technological and economic circumstances. Focussing on conceptual and practical shifts from the analog to the digital furthermore enables me to draw connections between analytic perspectives founded in dialectic materialism and contemporary theories foregrounding issues of immaterial labor. The important qualitative changes that practices and perceptions of appropriation have undergone are argue to significantly amplify the critical potential of all appropriative practices. Ultimately, my comparative analyses thus establish appropriation as an ideal site for effectively challenging – both in terms of form and content – the ingrained, restrictive notions of original genius and naturalized authorship-qua-ownership on which present cultures and technologies of global capitalism are so heavily based.

Keywords: appropriation
sampling
collage
found footage
Advisor: Cazdyn, Eric
Probing Exotic Superconducting Order Parameters Using Point-contact Andreev Reflection Spectroscopy

Point-contact Andreev reflection spectroscopy measurements were performed on various unconventional superconducting systems, as a function of temperature and magnetic field, to probe their superconducting order parameter symmetries. Low-temperature differential conductance measurements on the heavy-fermion system PrOs4Sb12 revealed multiple spectral features, including distinct evidence for gap nodes in the superconducting order parameter. A field-driven change in order parameter symmetry was also observed confirming the existence of multiple superconducting phases with different symmetries in PrOs4Sb12. The effect of Ru doping in Pr(Os1−xRux)4Sb12 was studied and the spectral evolution is interpreted in terms of multigap pairing. Our data which indicates that the nodal order parameter is rapidly suppressed by the introduction of Ru is consistent with other experimental results. Point-contact tips were fabricated from the high-Tc superconductor YBa2Cu3O7− (YBCO) and used to form high impedance junctions with CrO2 to determine how the formation of Andreev bound states is effected by spin polarization. Strong suppression of the d-wave Andreev reflection characteristics were observed in the YBCO/CrO2 junctions indicating that spin polarization prevents Andreev surface states from forming. The point contacts were estimated to be on the order of several nanometers in size, attesting to their microscopic nature and demonstrate the feasibility of using superconducting high-Tc tips as spin-sensitive nanoprobes of itinerant ferromagnets. Point-contact Andreev spectroscopy measurements were performed on single crystal samples of the heavy-fermion superconductor CeCoIn5. The temperature dependence of the differential conductance spectrum, measured down to 150 mK, showed multiple features whose characteristics depended on the junction impedance. Spectral analysis revealed two co-existing order parameters with nodal symmetry indicating a highly unconventional pairing symmetry in CeCoIn5. The magnetic field dependence of the differential conductance spectrum, where the applied field was parallel to the c-axis, was measured and the upper critical field observed was consistent with that determined by other experimental studies. Low-energy peak structures were observed in low-temperature differential conductance measurements performed on the itinerant ferromagnet ZrZn2. The temperature and field dependence of the spectra were measured and discussed within the context of surface superconductivity known to exist in ZrZn2.

Advisor: Wei, John
Anticonvulsant Effects of Omega-3 Polyunsaturated Fatty Acids in Rodents

The present research examined the hypothesis that omega-3 polyunsaturated fatty acids would increase seizure threshold in rats in vivo, and reduce neuronal excitability in mouse hippocampal slices. Seizure thresholds were measured in rats using the maximal pentylenetetrazol and electrical stimulation seizure tests following α-linolenic acid (ALA) or docosahexaenoic acid administration. ALA raised seizure threshold in the maximal PTZ seizure test, but this effect probably occurred because ALA displaced DHA from liver to the brain. DHA itself was therefore tested in the PTZ and electrical stimulation seizure tests. Direct administration of DHA by subcutaneous injection raised seizure thresholds in the PTZ seizure test, which models tonic-clonic attacks in humans. Dietary enrichment with DHA raised afterdischarge seizure thresholds in the cortex and amygdala, which model simplex and complex partial seizures in humans, although this effect took some time to occur. In vitro, the application of DHA also reduced the incidence of excitatory sharp waves in mouse hippocampal slices. This effect did not appear to be due to either an increase in GABAergic inhibitory tone, nor to a decrease in glutamatergic drive. The fatty acid composition of phospholipids and unesterified fatty acids were measured in the brain following microwave fixation in order to determine whether the effects of DHA on seizure thresholds were due to its de-esterification from the phospholipid membrane. The assay surprisingly revealed that subcutaneous administration of DHA at a dose that raised seizure threshold, increased unesterified arachidonic acid, but not unesterified DHA concentrations during seizures. The results of these studies support the hypothesis that DHA raises seizure threshold in rats, and reduces neuronal excitability in vitro. The effects of DHA on seizure threshold are possibly mediated by the de-esterification of arachidonic acid, which is known to have effects on the voltage-dependent sodium channel.

Keywords: Omega-3 polyunsaturated fatty acids
seizures
epilepsy
Advisor: Burnham, W. McIntyre