VictorJuiz/thesis huggingface dataset:
Exploring the Suitability of a Specifici Glucocorticoid Receptor Antagonist as a Tool in the Study of the Regulation of Rat Lung Alveolarization by Glucocorticoids

Background: Intracellular glucocorticoid receptors (GRs) mediate the regulation of lung development, including alveolarization, by glucocorticoids (GCs). One potential approach to determining the role of GC-GR signalling in alveolar formation would be by pharmacologic blockade. Hypothesis: CP472555, a novel GR antagonist with negligible anti-PR activity, is a suitable tool for the study of GC-GR regulation of rat alveolarization. Design/Methods: CP472555 doses needed to block GR were estimated in vitro in fetal rat lung primary cultures. Postnatally, a variety of doses were administered intraperitoneally over a range of days. Results: During postnatal days (PN)0-PN10, when GC levels are low, CP472555 induced changes consistent with GR agonist activity. While GC levels increase after PN11, animals exposed to CP472555 from PN11-PN21 exhibit changes consistent with anti-GR antagonist activity. Conclusion: CP472555 causes a degree of GR blockade sufficient to permit further pharmacological investigation of the role of endogenous GC-GR signalling at the end of alveolarization.

Keywords: alveolarization
glucocorticoids
rat lung development
glucocorticoid receptor antagonist
Advisor: Sweezey, Neil
A Generic Simulation Model to Improve Procedure Scheduling in Endoscopy Suites

In 2008 Ontario implemented a screening program for colorectal cancer, which drew attention to the increasing demand for colonoscopies in the province. This trend and the forecasted demand of the new screening program created a need to increase capacity in hospital endoscopy suites. This thesis addresses this need by investigating throughput gains from scheduling according to physician specific procedure durations in endoscopy suites. This is accomplished through the development of a scheduler and a generic discrete event simulation. Case study results show that physician specific scheduling can increase throughput in the endoscopy suite while reducing undertime and only slightly increasing overtime. They further indicate the trade off between a 1:2 and 1:1 physician to room ratio, finding that while a 1:1 ratio increases throughput by 33% over a 1:2 ratio, physicians are 1.5 times more productive under a 1:2 ratio.

Keywords: health care
simulation
endoscopy
colorectal cancer
throughput
overtime
undertime
hospital
Advisor: Carter, Michael W.
Characterizing Plasmacytoid Dendritic Cell Activation in the Context of HIV Stimulation

Plasmacytoid dendritic cells (pDCs) are important in innate and adaptive immune responses against viral infections, producing remarkable levels of type I interferon as well as other immunomodulatory cytokines. In vitro, pDCs can control replication of human immunodeficiency virus (HIV). Following natural challenge with simian immunodeficiency virus (SIV), PDCs infiltrate the vaginal mucosa. Despite this, pDCs are unable to prevent systemic dissemination of virus. To understand pDC activation immediately following HIV exposure, cytokine production and maturation of pDCs in response to HIV and a variety of other stimuli was compared. PDCs were found to respond suboptimally to HIV compared to other stimuli, with delayed interferon-alpha production, delayed maturation, and decreased production of inflammatory cytokines. Furthermore, HIV induced inhibitory signaling pathways in pDCs, suggesting subversion of the pDC response by HIV. Finally, different stimuli induced different early and late cytokine profiles, highlighting the complex and stimuli-dependant nature of the pDC response.

Keywords: HIV
pDC
Interferon
Cytokine
Advisor: Ostrowski, Mario
Concurrent Self-administration of Alcohol and Nicotine in an Operant Paradigm

Rationale and objectives: Alcohol and nicotine are the most commonly abused drugs and they are often taken together. To help address some of clinical issues regarding nicotine and alcohol co-dependence, a procedure in which rats self-administer nicotine intravenously and alcohol orally during the same operant session has been developed. Methods: Male Wistar rats were trained to self-administer alcohol (12%, w/v; 0.19 ml/delivery) or implanted with jugular catheters and trained to self-administer nicotine (30 μg/kg IV/infusion) by pressing a lever or were trained to self-administer both drugs, some with alcohol first, and others with nicotine first. Results: Animals readily coadministered alcohol and nicotine concurrently. Access to alcohol reduced nicotine selfadministration significantly. Conclusions: These results show that rats will self-administer relevant amounts of intravenous nicotine and oral alcohol concurrently. They also provide further support for the important relationship between nicotine and alcohol.

Keywords: Alcohol
Nicotine
Self-Administration
Drug abuse
Drinking
Smoking
Rats
Advisor: Le, Anh Dzung
The Role of Cholinergic Cortical Modulation from the Nucleus Basalis Magnocellularis in Visual and Olfactory Attention using the 5-Choice Serial Reaction Time Task

To date, research using rodent models has primarily dealt with the visual aspects of attention, while in the present research, we examined neurochemical modulation of attentional processes using both a visual and an olfactory five choice serial reaction time task (5-CSRTT). The nucleus basalis magnocellularis (NBM) in the basal forebrain is the primary source of cortical cholinergic input, which is thought to have an important modulatory effect on rat attentional processes. Thus, after successful acquisition of the task, the rats were subjected to either a cholinergic immunotoxic or sham lesion surgery of the NBM. Cortical cholinergic deafferentation of the cortical mantle was induced by bilaterally infusing 0.2 µg/µl of the cholinergic immunotoxin, 192 IgG-saporin, into the NBM (saporin-lesion). Reduction of cortical cholinergic modulation led to comparable attentional impairments in the saporin-lesion group, relative to the sham-lesioned group, on both visual and olfactory versions of the 5-CSRTT.

Keywords: acetylcholine
attention
5-CSRTT
Advisor: Rosa, Eve De
Governing the Restless and Young in Contemporary China: in Search for the Chinese Communist Party's Ruling Logic

This thesis explores one particular facet of contemporary state-society relationship in China, i.e. state-student relationship. By arguing against the popular observation that the Chinese Communist Party (CCP) retreated from Chinese universities as a way of winning students’ support, this thesis claims that the party-state has adopted a “bird cage” strategy in post-Tiananmen university governance. That is to say, the party-state has not only re-established and strengthened its control institutions in universities, but at same time expanded its zone of tolerance and created new outlets for students’ political enthusiasm and participation. A four-city, seven-university field survey was conducted, the result of which supports the view that the CCP’s post-Tiananmen governance strategy has been effective. Respondents agree that party’s governing institutions are resilient and play important roles in students’ life. They also seem to be in agreement with, at least as the survey results indicate, important political ideas promoted by the party-state.

Keywords: Chinese politics
Chinese students
Tiananmen
Chinese Communist Party
Authoritarianism
Chinese democracy
Advisor: Ong, Lynette
Falkenheim, Victor
Wong, Joseph
Characterization of Murine Intestinal Mucus Layer Gene Expression during Postnatal Maturation

The objective of this work was to characterize the expression pattern of the main intestinal mucin and glycosyltransferases genes in the distal ileum, caecum, proximal and distal colon of 5, 10, 15, 21, 25 and 32 days old mouse pups by real-time PCR. We found that all genes considered, except for Muc13 followed a spatio-temporal expression pattern. As a potential modulator of mucin gene expression, gut microbiota composition was also analyzed in the same animals. We found that neither the total bacterial numbers nor the relative abundance of the main microbial phyla vary after 21 days of age. The characterization of the postnatal intestinal mucus layer gene expression may aid to understand disease-related deviations and help designing nutritional interventions to sustain the intestinal barrier.

Keywords: Nutrition
Advisor: Comelli, Elena
The Relationship between Acute and Chronic Aerobic Exercise Response in Pre-hypertensive Individuals

Aerobic exercise is recommended as a lifestyle intervention to reduce blood pressure (BP) in individuals with elevated BP (SBP/DBP >120/80 mmHg). However, the BP response is highly variable after both acute (SBP/DBP: –27 to 9/-8 to 7mmHg) and chronic aerobic exercise (-20 to 9/ -11 to 11.3mmHg). We attempt to identify those who are resistant or responsive to training based on their responses to acute (one-bout) exercise. 17 prehypertensive (120 to 139/80 to 89mmHg) males and females (45-60yrs) underwent acute exercise assessments before and after an 8-week walking/jogging program. The magnitude of change in BP after acute exercise significantly correlated (r=.89, p < .01) with the magnitude of change in resting BP after the training. The antihypertensive mechanisms (total peripheral resistance, baroreflex sensitivity) for acute exercise were not correlated to those for chronic exercise. Central cardiovascular controls may link BP reductions after both acute and chronic exercise.

Keywords: Exercise, blood pressure
pre-hypertension, post exercise hypotension
Advisor: Thomas, Scott
Effects of Cyclic Hydraulic Pressure on Osteocytes

Bone changes composition and structure to adapt to its mechanical environment. Osteocytes are putative mechanosensors responsible for orchestrating the bone remodeling process. Recent in vitro studies showed that osteocytes could sense and respond to substrate strain and fluid shear. However the capacity of osteocytes to sense cyclic hydraulic pressure (CHP) associated with physiological mechanical loading is not well understood. In this study, osteocyte-like MLO-Y4 cells were subjected to CHP of 68 kPa at 0.5 Hz, and the effects of CHP on intracellular calcium concentration, cytoskeleton organization, mRNA expression of genes related to bone remodeling, and osteocyte apoptosis were investigated. The results indicate that osteocytes could sense CHP and respond by increased intracellular calcium concentration, altered microtubule organization, an increase in COX-2 mRNA level and RANKL/OPG mRNA ratio, and decreased apoptosis. Therefore cyclic hydraulic pressure in bone a mechanical stimulus to osteocytes and may play a role in regulating bone remodeling.

Keywords: biomechanics
bone
mechanotransduction
osteocyte
Advisor: You, Lidan
The Dosimetric Consequences of Intensity Modulated Radiotherapy for Cervix Cancer - the Impact of Organ Motion, Deformation and Tumour Regression

Cervix cancer affects women of all ages and causes significant morbidity and mortality. Locally advanced disease is curable with radiotherapy (RT) in about 50% of patients, although often at the expense of serious side effects. In order to improve the therapeutic ratio of tumour control versus normal tissue toxicity, conformal intensity-modulated radiotherapy (IMRT) is being investigated. However, inter- and intra-fractional motion of cervix cancer can contribute to both geographical miss of the target and overdosing of surrounding normal tissues, particularly in the setting of conformal IMRT with steep dose gradients. Defining the target volume accurately and understanding the dose consequence of these complex intra-pelvic organ dynamics during external beam radiotherapy forms the essential foundations for future treatment optimization and adaptation. This in turn will lead to improvements in tumour control and disease-free survival while minimising treatment toxicity.

Keywords: cervix cancer
intensity modulated radiotherapy
consensus guidelines
dose accumulation
organ motion
Advisor: Milosevic, Michael
Fyles, Anthony
Embedded Systems Development for SFL Satellites

The work described in this thesis summarizes the author's contributions to the design, development, and testing of embedded systems for SFL spacecraft. The unique environment of space and the constraints it imposes on embedded systems is described, and the testing methodologies employed to qualify spacecraft hardware for this environment are presented. The implementation of an automated functional test framework for SFL's Generic Nanosatellite Bus satellites and the role it plays in the spacecraft development cycle is also discussed. Application software and device drivers in support of the BRITE mission were designed and developed. Finally, a controller was implemented for thermal control of the BRITE optical instrument. These contributions play an important role in the continual efforts to expand access to space and to prove the feasibility of the microspace approach in doing so.

Keywords: satellite
embedded systems
Advisor: Zee, Robert E.
Microstructure Evolution and Mechanical Properties of Electroformed Nano-grained Nickel upon Annealing

Nano-grained nickel produced by electroforming technique was investigated for its microstructure evolution and mechanical properties upon annealing. It was found that during low temperature annealing (T<250 oC), electroformed nano-grained nickel showed scattered and isolated abnormal grain growth, followed by a major abnormal grain growth at 320 oC. A secondary abnormal grain growth, featuring faceted grain boundaries, was observed at a higher annealing temperature (T=528 oC). A semi-in-situ observation using optical microscopy was conducted to track the movement of the faceted grain boundaries, and it was found that these boundaries were mostly immobile. The mechanical properties under various annealing conditions were studies using hardness and tensile testing. The hardness was observed to decrease with increasing annealing temperature. The material became very brittle after annealing at 320 oC or higher temperatures. Fractography investigation showed that the brittleness is caused by intergranular fracture.

Keywords: nanocrystalline nickel
microstructure evolution
mechanical properties
electroformed
Advisor: Wang, Zhirui
A Population-based Study on the Association of Standardized Protocols in the Emergency Department for Childhood Asthma with Outcomes in Ontario, Canada

Objectives: To describe the use of standardized protocols (SPs) in emergency departments (EDs) across Ontario for childhood asthma and analyze the association of SPs with hospital admissions, 7-day ED re-visits, and outpatient follow-up visits. Methods: Population-based retrospective cohort study using health administrative data and survey data. EDs were categorized as having the optimal, other, or no SP for each outcome. Associations were tested with generalized estimating equations. Results: Between 2006/04/14-2009/02/28, 46,510 children with asthma were seen in 146 EDs, with 43 (29.5%) having SPs. Children treated in EDs with the optimal SP compared to no SP had no significant differences in hospital admissions (AOR 1.17; 95% CI 0.91, 1.49) or ED re-visits (AOR 1.09; 95% CI 0.85, 1.40) but were more likely to have follow-up visits (AOR 1.27; 95% CI 1.02, 1.59). Conclusions: SPs for childhood asthma are not common in Ontario EDs and had little impact on outcomes measured.

Keywords: Asthma
Pediatric
health services research
epidemiology
guidelines
clinical pathways
standardized protocols
medical directives
pre-printed orders
Advisor: Guttmann, Astrid
Modeling of Flexible Aircraft for 3D Motion-based Flight Simulators

This thesis compares the results of two of the more popular flexible aircraft modeling formulations, the mean-axes method and the fixed-axes method, for application in real-time motion simulators. First, the time-domain equations of motion for an elastic body using the fixed-axes are derived. Subsequently, the mean-axes equations are derived by making a few assumptions from the fixed-axis equations. The two formulations are then implemented for a scaled-up beam model of a Cessna Citation aircraft, with the deformations represented by the modal expansion of the whole aircraft from their respective constrained and free-free finite element solutions. Time-domain results, consisting of the acceleration, velocity, and attitude of a point on the aircraft body, are obtained in both models at two beam-stiffness configurations using a quasi-steady aerodynamic model for a single maneuver at one flight condition. The two methods produced similar results with the fixed-axes formulation producing slightly more accurate results.

Keywords: Flexible Aircraft
Simulation and Modeling
Advisor: Grant, Peter R.
Exploration of Corporate Governance between Developed Nations and The People's Republic of China

This article explores the corporate governance in the developed countries and China from a comparative perspective. Following the analysis of principal-agent model, this article examines the dispersion-to-concentration ownership span to explore its influence on the majority/minority ownership and the shareholder/manager conflicts. It compares the positive and negative edges of concentrated shareholding with empirical analysis of Canada, the U.S., and China, and finds the different roles of institutional shareholders in various countries. This article then turns to two-tier agency model which is another way to enhance corporate governance. Compared with the vertical construct with the vanguard of Germany, it illustrates that the supervisory board in china is situated parallel to the board of directors and loses the supervising purpose. This article finally explores the independent director system which has obtained credits in the developed countries. While in China, it lacks legal protection and is not effective as expected.

Keywords: corporate governance
China
Advisor: MacIntosh, Jeffrey G.
Sprawl in Canada and the United States

The purpose of this thesis is to ascertain (1) whether suburban sprawl is as widespread in Canadian metropolitan areas as in their American counterparts, and (2) whether Canadian government policies, and in particular Canadian municipal land use and transportation policies, encourage sprawl. The thesis concludes that sprawl is less widespread in two respects. First, Canadian central cities have not declined to the same extent as American central cities. Second, urban and suburban Canadians are less dependent on automobiles than are Americans. The thesis goes on to point out that in Canada, as in the United States, government land use and transportation policies often encourage sprawl.

Keywords: sprawl
Advisor: Weinrib, Arnold
A Platform to Monitor Tumor Cellular and Vascular Response to Radiation Therapy by Optical Coherence Tomography and Fluorescence Microscopy in vivo

Radiotherapy plays a significant role in cancer treatment, and is thought to be curative by mainly killing tumor cells through damage to their genetic material. However, recent findings indicate that the tumor’s vascular blood supply is also a major determinant of radiation response. The goals of this thesis are to: (1) develop an experimental platform for small animals to deliver ionizing radiation and perform high-resolution optical imaging to treatment targets, and (2) use this toolkit to longitudinally monitor the response of tumors and the associated vasculature. The thesis has achieved: (1) customization of a novel micro-irradiator for mice, (2) technical development of an improved optical coherence tomography imaging system, (3) comprehensive experimental protocol and imaging optimization for optical microscopy in a specialized animal model, and (4) completion of a feasibility study to demonstrate the capabilities of the experimental platform in monitoring the response of tumor and vasculature to radiotherapy.

Keywords: Optical Coherence Tomography
Fluorescence Microscopy
Radiation Therapy
Vasculature
Laser
Advisor: Vitkin, Alex
The Influence of Synoptic Weather Conditions on Weekday-weekend Effect of Extreme Ground-level Ozone Events in the Toronto area

Ground-level ozone (O3) is a familiar pollutant because it is associated with summer haze and smog alerts. The 2000-2008 weekday-weekend variations of ozone concentration were examined in relation to the Toronto weather conditions. The goal of this work is twofold: (1) To determine whether extreme ozone events were associated with specific weather conditions, (2) To determine whether the weekday-weekend effect of extreme ozone events could be detectable during the nine-year study period. The results show that in the study period, there were totally 313 days having extreme ground-level ozone events with ozone concentration ≥ 80 ppb, which is the current Ontario Ambient Air Quality Criterion for ozone concentration, in the four selected Toronto sites. Additionally, the weather condition mainly associated with these 313 days was the Dry Tropical one. This study also shows the phenomenon of the weekday-weekend effect of extreme ozone events in the past nine years in Toronto.

Keywords: Extreme Ground-level Ozone Event
Synoptic Weather Conditions
Weekday-Weekend Effect
Toronto
Advisor: Gough, William A.
Genetic Factors Influencing BCG Vaccine Properties

Tuberculosis is a re-emerging global health problem. Bacille Calmette-Guerin (BCG), the available vaccine against the disease, is only effective short term and is associated with adverse reactions clinically. The development of new effective vaccines will require an understanding of virulence, immunogenic factors and the beneficial immune responses induced in the human host. My thesis investigates phoP and whiB3, two genes associated with virulence and immunogenicity in Mycobacterium tuberculosis. Study of PhoP in a natural phoP mutant, BCG-Prague, and in the clinically safe BCG-Japan, shows that over-expression of PhoP increases the immunogenicity of these vaccine strains. In addition, I found that WhiB3 impacts carbon metabolism in BCG-Birkhaug and BCG-Sweden, although the effect of this on virulence in vivo is still unclear. The characterization of genes involved in virulence and immunogenicity allows us to develop novel approaches for improving the efficacy of BCG, which has important implications for future TB vaccine development.

Keywords: BCG
Mycobacterium tuberculosis
PhoP
WhiB3
vaccine
Advisor: Liu, Jun
Protein Hypercitrullination, a Basic Mechanism of Demyelinating Diseases

Multiple sclerosis (MS) is a complex disease of the human central nervous system (CNS) involving the patchy destruction of the myelin sheath. Previous studies have found a consistent biochemical change in MS normal appearing white matter (NAWM) i.e. the increased citrullination of myelin basic protein (MBP) resulting in decreased myelin compaction. This process is facilitated by the enzyme family of peptidylarginine deiminases (PADs), of which PAD2 and PAD4 are expressed in mouse and human brain white matter. Therefore, we propose the inhibition of PAD enzymes would reverse protein hypercitrullination and represents a potential treatment for MS. Treatment with 2-chloroacetamidine (2CA), an active site inhibitor of PAD, attenuated diseases in four independent mouse models of MS associated with decreased PAD activity level, normalized peptidylcitrullination, and improved myelin morphology. Therefore, protein hypercitrullination may be a basic mechanism implicated in both neurodegenerative and autoimmune models of MS.

Keywords: multipls sclerosis
citrullination
peptidylarginine deiminases
2-chloro acetamidine
Advisor: Moscarello, Mario