VictorJuiz/thesis huggingface dataset:
On Globalization and Civil Society: Mediating Spatial Practice in Twenty-first-century Latin America

“On Globalization and Civil Society: Mediating Spatial Practice in Twenty-first-century Latin America” explores the tensions between globalization and civil society from a multi-geographical and multidisciplinary angle. The dissertation is informed by theories of space, power, identity, citizenship, and postmodernity, as well as mediatic and socio-political analyses of conditions that have consistently challenged democracy and the formation of a just civil society specifically in the Colombian and Mexican contexts but throughout Latin America as well. I argue that national institutions fundamental to the formation of knowledge and the construction of identity--namely national citizenship, geopolitical and symbolic borders, and the national media--impose undue limits and power on globally affected individuals. After acknowledging and analyzing the dehumanizing way in which these national institutions limit individual freedoms and participation within local and global public spheres, I take a more hopeful stance as I explore humanizing instances that transcend victimization through the imagination and creation of alternative social orders that destabilize traditional apparatuses of authority through agency-enhancing initiatives. Through close readings of contemporary Colombian and Mexican narrative by Héctor Abad Faciolince, Jorge Franco, Heriberto Yépez, and Luis Humberto Crosthwaite, and a case study of Un Pasquín, Vladimir Flórez’s independent alternative Bogotá media project, I call for a new understanding of the possibilities of the twenty-first-century public sphere in Latin America. I contend that by subverting dominant paradigms of power, these alternative spheres provide a new model from which to think and advance a just global order. In short, I argue that, despite globalization’s mostly deleterious consequences for the world’s most at risk local populations, the formation of a more humanizing spatial and mediatic practice that fosters alternative public spheres responsive to the human need for individual agency and subjectivity, though seemingly unattainable, is in fact possible.

Keywords: Colombia
Mexico
Advisor: Davidson, Robert A.
Challenging the Dominant Discourse of ‘Welfare Dependency’: A Multi-episode Survival Analysis of Ontario Works Spells

This dissertation examines the dominant discourse of welfare dependency and its implications for lone mothers in Ontario, Canada. This hegemonic discourse has been instrumental in positioning lone mothers as deviant, pathologically flawed and ineffective citizens. Using a repeated survival analysis, I examine the spells of participants identifying the significant variables influencing social assistance exit rates. Social constructionism and critical feminism are the theoretical lenses underpinning the analysis. The quantitative study examines the current composition of the Ontario Works caseload, interrogates the legitimacy of the welfare dependency supposition, debunks numerous social constructions surrounding welfare receipt and highlights the barriers impeding participants. The study culminates with a new understanding to counter the welfare dependency paradigm, recognizing the overlooked provisioning work of women in the neoliberal post welfare state.

Keywords: social assistance, welfare, Ontario, Canada, social provisioning, lone mothers, social exclusion, social capital, new social movements, citizenship
Advisor: Neysmith, Sheila
Architecture and Identity: The Occupation, Use, and Reuse of Mughal Caravanserais

Life, individual and collective, exists in reference to what came before; my research into the life histories of places explores the cultural threads which tie us to places and which allow us to make personal and collective connections between the past and the present. Understanding these life histories helps us understand the value and power historic places have in the world today. This thesis focuses on Mughal caravanserais from northwestern Pakistan, examining how they were initially intended to be used during the Mughal rule of South Asia and how they were reused in the periods that followed (Sikh, Afghani, British, and Pakistani). Caravanserais are walled arcaded buildings where travelers could stop briefly. After the Mughal Empire declined, caravanserais were reused by local people and/or taken over by subsequent governing bodies. Surviving structures are still used today, although their original appearance and functions have been altered to serve new purposes. My research is part of the Caravanserai Networks Project, directed by Dr. Heather Miller, University of Toronto. I develop my survey method through comparative study of two caravanserais, Gor Khuttree and Pakka Khanpur. Using information from architectural survey, historic documents, and photographs, I create three-dimensional architectural models of Gor Khuttree’s occupations. I use access and planning analysis to characterize the use and alteration of the sites’ architecture. Conceptually, I develop a tripartite formation of place: formed, in practice, and in memory. As a multifaceted place, I consider Gor Khuttree’s life history; the occupations, identities, and memories associated with the site through time. The City of Peshawar, central to this research, was at the time of writing in Pakistan’s North West Frontier Province. This province was renamed Khyber Pakhtunkhwa on April 15th, 2010. This research continues to refer to the province by its former name. This ensures continuity with published documents and the organizational names used by colleagues in Pakistan at the time of research.

Keywords: Mughal
Caravanserai
South Asia
Architecture
Survey
Identity
life history
Place
Sikh
British
Pakistan
India
Memory
Peshawar
NWFP
Archaeology
Anthropology
History
Advisor: Miller, Heather
Posttraumatic Growth in Survivors of Breast Cancer: The Role of Dispositional Optimism, Coping Strategies, and Psychosocial Interventions

This study investigated the experience of posttraumatic growth for breast cancer survivors who volunteered to complete a survey (N = 277). A number of participants also provided narrative responses containing their personal experiences of breast cancer (N = 100). Demographic characteristics, breast cancer variables, optimism, coping strategies, and participation in psychosocial interventions were examined in relation to posttraumatic growth as measured by the Posttraumatic Growth Inventory. Differences between those who participated in psychosocial interventions versus those who did not were examined. The relation of coping strategies utilized and optimism level to interventions accessed was also of interest. Predictors of posttraumatic growth were examined via generalized linear modeling. Finally, the narrative responses were investigated by means of content analysis. The results provided evidence that demographic characteristics (i.e., affiliation with religion and lower levels of education) and one breast cancer variable (i.e., increased number of years since diagnosis) were related to experiences of posttraumatic growth for breast cancer survivors. Use of active forms of coping, optimism level, and participation in recreational interventions were also related to posttraumatic growth. Those who participated in psychosocial interventions were more likely to be employed and have higher levels of education than those who did not access interventions. Use of several active coping strategies and one passive coping strategy increased the likelihood of accessing a number of different psychosocial interventions. Posttraumatic growth was predicated by affiliation with religion, lower levels of education, increased number of years since diagnosis, knowledge of breast cancer stage, higher levels of optimism, and use of one passive and two active coping strategies. Many of these findings support previous research, however some conflict with prior results. The narrative responses contained information regarding diagnosis and treatment, impact of breast cancer, coping with breast cancer, and support accessed. Although the negative impact of breast cancer was discussed in terms of short and long-term, positive long-term changes were also reported. A variety of coping strategies and supports were utilized by the participants as well.

Keywords: posttraumatic growth
breast cancer
psychosocial intervention
optimism
coping strategies
Advisor: Stermac, Lana
Conformal Heating of the Prostate for the Treatment of Localized Cancer using MRI-guided Transurethral Ultrasound

Prostate cancer is the most prevalent cancer and the third-leading cause of cancer-related death among men in the developed world, with the number of cases expected to double within the next 15 years. Conventional therapies offer good control of local disease but are associated with high complication rates reducing long-term health-related quality-of-life significantly. MRI-guided transurethral ultrasound therapy has emerged as an attractive, minimally-invasive alternative for the treatment of localized prostate cancer, where the entire gland is heated to temperatures sufficient to cause irreversible thermal coagulation. A device inserted in the urethra uses multiple ultrasound transducers to produce directional heating patterns directly in the prostate. Adjusting the ultrasound power, frequency and device rotation rate enables high spatial control of the thermal lesion. MRI provides information essential to the accurate targeting of the prostate; anatomical images for device positioning and treatment planning, and quantitative temperature measurements within the prostate to compensate for dynamic tissue changes, using feedback control. This thesis develops a complete treatment delivery strategy for producing conformal regions of thermal coagulation shaped to whole-gland prostate volumes, while limiting the thermal impact to the surrounding important anatomy. First, acoustic and thermal simulations incorporating a novel temperature feedback controller were used to model and shape regions of coagulation to human prostate geometries with a high degree of accuracy. Second, treatment delivery strategies were developed and simulated to reduce thermal injury to the surrounding anatomy, below the threshold for sustained damage. Third, experiments in tissue-mimicking gel phantoms confirmed the predictive accuracy of the simulations and the feasibility of producing conformal volumes of coagulation using transurethral ultrasound devices and MRI-temperature feedback. This work forms the basis of clinical treatment delivery methods and supports the use of the simulations as a planning tool to enhance the inherent compromise between safety and efficacy on a patient-specific basis.

Keywords: MRI-guided ultrasound therapy
Feedback temperature control
Prostate therapy
Advisor: Bronskill, Michael
Modular Approach to Adipose Tissue Engineering

Despite the increasing clinical demand in reconstructive, cosmetic and correctional surgery there remains no optimal strategy for the regeneration or replacement of adipose tissue. Previous approaches to adipose tissue engineering have failed to create an adipose tissue depot that maintains implant volume in vivo long-term (>3 months). This is due to inadequate mechanical properties of the biomaterial and insufficient vascularization upon implantation. Modular tissue engineering is a means to produce large volume functional tissues from small sub-mm sized tissues with an intrinsic vascularization. We first explored the potential of a semi-synthetic collagen/poloxamine hydrogel with improved mechanical properties to be used as the module biomaterial. We found this biomaterial to not be suitable for adipose tissue engineering because it did not support embedded adipose-derived stem cell (ASC) viability, differentiation and human microvascular endothelial cell (HMEC) attachment. ASC-embedded collagen gel modules coated with HMEC were then implanted subcutaneously in SCID mice to study its revascularization potential. ASC cotransplantation was shown to drive HMEC vascularization in vivo: HMEC were seen to detach from the surface of the modules to form vessels containing erythrocytes as early as day 3; vessels decreased in number but increased in size over 14 days; and persisted for up to 3 months. Early vascularization promoted fat development. Only in the case of ASC-HMEC cotransplantation was progressive fat accumulation observed in the module implants. Although implant volume was not maintained, likely due rapid collagen degradation, the key result here is that ASC-HMEC cotransplantation in the modular approach was successful in creating vascularized adipose tissue in vivo that persisted for 3 months. The modular system was then studied in vitro to further understand ASC-EC interaction. Coculture with ASC was shown to promote an angiogenic phenotype (e.g. sprouting, migration) from HUVEC on modules. RT-PCR analysis revealed that VEGF, PAI-1 and TNFα was involved in ASC-EC paracrine signalling. In summary, ASC-HMEC cotransplantation in modules was effective in rapidly forming a vascular network that supported fat development. Future work should focus on further elucidating ASC-EC interactions and developing a suitable biomaterial to improve adipose tissue development and volume maintenance of engineered constructs.

Keywords: Adipose Tissue Engineering
Adipose-derived Stem Cells
Regenerative Medicine
Biomaterials
Cotransplantation
In Vivo
Revascularization
Hydrogel
Advisor: Sefton, Michael
Woodhouse, Kimberly A.
Molecular Characterization of Hereditary Spherocytosis Mutants of the Cytoplasmic Domain of Anion Exchanger 1 and their Interaction with Protein 4.2

Anion exchanger 1 (AE1) is a red cell membrane glycoprotein that associates with cytoskeletal protein 4.2 in a complex bridging the cell membrane to the cytoskeleton. Disruption of this linkage results in unstable erythrocytes and hereditary spherocytosis (HS). Three HS mutations (E40K, G130R and P327R) in the cytoplasmic domain of AE1 (cdAE1) result in a decreased level of protein 4.2 in the red cell yet maintain normal amounts of AE1. Biophysical analyses showed the HS mutations had little effect on the structure and conformational stability of the isolated domain. However, the conformation of the cytoplasmic domain of the kidney anion exchanger, lacking the first 65 amino acids including a central -strand, was thermally destabilized relative to cdAE1 and had a more open structure. In transfected human embryonic kidney (HEK)-293 cells the HS mutants had similar expression levels as wild-type AE1, and protein 4.2 expression level was not dependent on the presence of AE1. Protein 4.2 localized to the plasma membrane with wild-type AE1, the HS mutants of AE1, the membrane domain of AE1 and kidney AE1, and to the ER with Southeast Asian ovalocytosis AE1. A fatty acylation mutant of protein 4.2, G2A/C173A, could not localize to the plasma membrane in the absence of AE1. Subcellular fractionation showed wild-type and G2A/C173A protein 4.2 were mostly associated with the cytoskeleton. Co-immunoprecipitation and Ni-NTA pull-down assays revealed impaired binding of protein 4.2 to HS mutants compared to AE1, while the membrane domain of AE1 was unable to bind protein 4.2. These studies show that HS mutations in cdAE1 cause impaired binding of protein 4.2, without causing gross structural changes in the domain. The mutations change the binding surface on cdAE1 by the introduction of positive charges into an otherwise acidic domain. This binding impairment may render protein 4.2 more susceptible to degradation or loss during red cell development.

Keywords: anion exchanger 1
protein 4.2
erythrocyte
cytoskeleton
protein-protein interactions
protein structure and stability
red blood cell
Advisor: Reithmeier, Reinhart A. F.
Automated Methods in Chemical Risk Assessment

In this thesis, topics in chemical hazard and risk assessment are explored through the use of multimedia mass balance models and high-throughput chemical property prediction methods. Chemical hazard metrics, as calculated by environmental fate and transport simulations, are investigated to determine the validity of two common simplifying assumptions in the underlying models; the use of octanol as a surrogate for organic matter and the use of environmental parameters that do not vary in time. A major finding is that the use of these common simplifying assumptions in multimedia mass balance models has little effect on chemical risk assessment, provided that chemicals are ranked relative to each other rather than a predetermined cutoff value. Chemical properties relevant to hazard and risk are collected, and applied in a large-scale chemical hazard assessment to derive a short list of potential Arctic contaminants. Several further data needs are identified; these are widely applicable and easily calculable metrics for chemical biodegradation, toxicity and emissions. A new method of predicting chemical properties is presented to assist in meeting these data needs. The method automatically creates predictive, quantitative relationships between the structures and properties of chemicals that are comparable to similar relationships created with expert judgement.

Keywords: chemical risk assessment
environmental modelling
Advisor: Wania, Frank
Inhibitory Control of Muscle Activity in Sleep

In this thesis, I examined the inhibitory control of REM sleep motor activity using both a pharmacological rat model and a genetic mouse model. I characterized the role for GABA and glycine in mediating the REM-specific suppression of muscle activity as well as their involvement in regulating the phasic muscle twitches that punctuate this atonia. Based on four specific research objectives, the following conclusions were drawn: 1. REM atonia is not directly mediated by glycinergic or GABAA-mediated inhibition. These data refute the prevailing hypothesis that REM atonia is caused by glycinergic inhibition. These receptors are, however, important in the regulation of phasic muscle twitch activity. 2. GABAB receptors can modulate REM atonia but only when acting in concert with GABAA and glycine receptors. Blockade of all three receptor types results in a partial reversal of REM atonia, suggesting a functional interaction is occurring between these receptors during REM sleep. 3. The phasic glycinergic/GABAA-mediated inhibitory drive present in REM sleep regulates the temporal pattern of phasic twitch activity that is seen across this state. I hypothesize that this progressively decreasing inhibitory input counteracts a gradually increasing excitatory input to shape the temporal distribution of muscle twitches across REM sleep. 4. A loss of normal inhibitory function may play a causal role in the pathology of REM sleep behaviour disorder (RBD), the sleep disorder characterized by excessive motor activity in REM sleep.

Keywords: sleep
motoneuron
glycine
GABA
Advisor: Peever, John H.
Regulation of Early T-cell Development and Commitment by HEB

Early T-cell development is regulated by a complex interplay between transcription factors and developmental cues which ensure that functional T-cells are produced within the thymus. Early thymocytes integrate these signals in a step-wise fashion that progressively restricts their lineage potential as they transition through the early stages of T-cell development. Gene knockout studies have shown that the E-protein transcription factor HEB is required for normal thymocyte development. Furthermore, many additional key regulators such as Notch1 have been identified, but the connections among them and their specific roles in early T-cell development have not been well established. In this thesis, I set out to determine the specific roles of HEB at the beta-selection checkpoint and to establish connections between HEB and the key regulators within the gene regulatory network that orchestrates early T-cell development. To facilitate these studies, I generated a series of new mouse models including HEBAlt transgenic mice that express a short form of HEB called HEBAlt, which enabled me to answer specific questions and examine rare populations. First, my studies of HEB-/- mice allowed me to identify an early block in T-cell development, which was alleviated upon the addition of an HEBAlt transgene. Furthermore, I identified pTa and CD3e signalling as specific targets of HEBAlt during -selection. Second, my studies on HEB-/- mice revealed that they have a defect in T-cell commitment, with compromised Notch1 function and a tendency to become DN1-like cells. Moreover, the DN1-like cells could be induced to differentiate into thymic NK cells, revealing a role for HEB in the T/NK cell lineage decision. This study has revealed a new set of interactions among HEB, Notch1, and GATA3 that regulate the T-cell fate choice in developing thymocytes. Unexpectedly, my studies have also provided evidence for a role of HEBAlt in lymphomagenesis, highlighting the strict regulation of E-protein function that is necessary to ensure normal T-cell development.

Keywords: T-cell development
hematopoiesis
E-proteins
HEB
thymus
TCRb-selection
Advisor: Anderson, Michele K.
Criminal Law and the Development of the Assizes of the Crusader Kingdom of Jerusalem in the Twelfth Century

The legal treatises of the Kingdom of Jerusalem were written in the thirteenth century, when most of the kingdom had been re-conquered by the Muslims. There are no treatises from the twelfth century, when the kingdom was at its height. The thirteenth-century jurists claimed that the kingdom had always had written laws, but they may have been making this up for political purposes. The treatises also discuss issues important to the noble class of which the jurists were a part: property rights and the feudal services owed to the king, as well as the proper way to plead their cases in court. But what do they say about criminal law, and laws for the lower classes? How were crimes tried and punished in the twelfth century, and did this differ from the laws recorded in the thirteenth century? Chapter one deals with the different treatises, and their claim that there was a set of laws called “Letres dou Sepulcre” in the twelfth century. The most important of the treatises for criminal law, the assizes of the burgess court, is examined in detail. Chapter two looks at the small number of laws that survive from the twelfth century, in charters, the canons of the Council of Nablus, and the chronicle of William of Tyre. Chapter three is a study of other descriptions of crusader law in the twelfth century, including those by Christian and Muslim pilgrims, and especially the observations of Usama ibn Munqidh. These accounts are tied together by the common theme of theft and the ways that thieves could be punished. Chapter four deals with cases mentioned by thirteenth-century sources, including theft, assault, and prostitution, but especially cases that led to trials by battle. The usefulness of such trials for dating some of the laws is also examined. The conclusion demonstrates that certain parts of the assizes relating to criminal law must have already existed in the twelfth century, and offers some tentative ideas about the specific origin of the laws. Avenues for future research are also introduced.

Keywords: crusades
Kingdom of Jerusalem
medieval law
Advisor: Gervers, Michael
Armstrong, Lawrin
Meyerson, Mark
Northrup, Linda