VictorJuiz/thesis huggingface dataset:
Exploiting the Non-innocent Ligand Reactivity of Metal Bis-dithiolenes: Towards the Catalytic Synthesis of Chiral Thioether Ligands and other Synthetic Targets

Asymmetric catalysis is one of the most effective ways to control a target molecule’s stereochemistry. Through the development of a wide variety of chiral transition metal complexes, synthetic chemists are given the tools they need to synthesize the desired enantiomer of numerous compounds. This work focuses on exploiting the non-innocent ligand reactivity of metal bis-dithiolenes with multiple conjugated π systems in order to synthesize chiral ligands. Recent work has shown that platinum bis-dithiolene reacts with dienes stereoselectively to form a racemic mix of C2 -chiral thioether ligands. The present contribution will show approaches to synthesizing chiral dienes and organometallic complexes with potential applications for asymmetric allylic substitution reactions. Dienes with various chiral auxiliaries were reacted with platinum dithiolene and monitored through NMR spectroscopy. Attempts to synthesis palladium bis-dithiolene complexes, not previously seen in the literature were made, and the reaction of α-β unsaturated ketones with metal bis-dithiolenes was explored.

Keywords: Dithiolenes
Chiral Ligand
Ligand Non Innocence
Asymmetric Catalysis
Platinum
Palladium
Hetero-Diels-Alder
Advisor: Fekl, Ulrich
Evaluation of Oral Neutrophil Levels as a Quantitative Measure of Periodontal Inflammatory Load in Patients with Special Needs

Purpose: To validate and assess the feasibility of using an assay of oral neutrophils to measure periodontal inflammation in uncooperative patients with special needs. Methods: Periodontal examination and neutrophil counts derived from oral swabs were performed on patients with special needs having comprehensive dental treatment under general anaesthesia (GA). The conventional periodontal measurements were compared to neutrophil levels while patients were under GA, and later at their recall examination. Results: Forty-nine patients were assessed under GA and 30 (61%) returned for recall examination. Spearman’s correlation allowed for comparisons between periodontal parameters and oral neutrophil counts. Despite limited cooperation, it was possible to acquire neutrophils (using swabs) for all patients that presented for recall examination in the ambulatory dental clinic. Conclusions: Oral neutrophil levels correlated significantly with conventional parameters of gingival inflammation and may serve as a standardized method for clinical assessment of periodontal diseases in the special needs population.

Keywords: neutrophil
periodontal disease
special needs
inflammatory load
quantification
oral swab
gingival inflammation
diagnostics
hospital dentistry
Advisor: Sigal, Michael
Tenenbaum, Howard
Shape Optimization and Modular Discretization for the Development of a Morphing Wingtip

Better knowledge in the areas of aerodynamics and optimization has allowed designers to develop efficient wingtip structures in recent years. However, the requirements faced by wingtip devices can be considerably different amongst an aircraft’s flight regimes. Traditional static wingtip devices are then a compromise between conflicting requirements, resulting in less than optimal performance within each regime. Alternatively, a morphing wingtip can reconfigure leading to improved performance over a range of dissimilar flight conditions. Developed within this thesis, is a modular morphing wingtip concept that centers on the use of variable geometry truss mechanisms to permit morphing. A conceptual design framework is established to aid in the development of the concept. The framework uses a metaheuristic optimization procedure to determine optimal continuous wingtip configurations. The configurations are then discretized for the modular concept. The functionality of the framework is demonstrated through a design study on a hypothetical wing/winglet within the thesis.

Keywords: morphing
wingtip
discretization
optimization
Advisor: Behdinan, Kamran
Importing U.S. Establishment Clause Principles in Canada: Changing the Debate on Funding for Faith-based Schools in Ontario

Ontario is the only Province that publicly funds Roman Catholic separate schools, while providing no public funds to any other faith-based schools. This thesis explores the history, politics, past and current litigation surrounding Ontario’s education system. It looks at the education systems in other Canadian Provinces. I argue that focusing the debate around equality and fairness, and preventing the perceived establishment of the Roman Catholic Church in respect for multiculturalism will allow for an appealable solution. In many church and state cases, the Supreme Court of Canada made its decisions similar to the U.S. regarding the establishment of religion in public schools. This thesis suggests that Ontario should adopt a bilateral constitutional amendment that includes Establishment Clause principles similar to the U.S. Constitution. It concludes that the treatment of all faith-based schools equally, respects the historic rights of the Roman Catholic schools, and demonstrates a commitment to multiculturalism in Canada.

Keywords: constitutional law
religion
Advisor: Schneiderman, David
Identification and Characterization of Small Molecule Inhibitors of Polynucleotide Kinase 3'-Phosphatase

DNA lesions arise constantly in cells and are repaired by a variety of DNA repair pathways. Polynucleotide kinase 3’-phosphatase (PNKP) aids repair by phosphorylating 5’-hydroxyl DNA termini and dephosphorylating 3’-phosphate DNA termini for the completion of repair by DNA ligases. This activity is critical in vivo because DNA breaks do not usually possess ligatable termini. PNKP knockdown sensitizes cells to several DNA damaging agents, including the topoisomerase I (TOP1) inhibitor camptothecin - analogs of which are being developed into chemotherapeutic drugs - because the resolution of stalled TOP1-DNA complexes requires processing by PNKP. We hypothesize that small molecule inhibitors of PNKP could bolster the effects of radio- and chemotherapies on cancer cells. I have identified eight compounds that effectively inhibit human PNKP and, with reduced potency, T4 PNK in vitro. These compounds act by reversibly inhibiting the substrate-enzyme interaction but they do not appear to sensitize U2OS cells to camptothecin.

Keywords: polynucleotide kinase
small molecule inhibitors
DNA repair
Advisor: Durocher, Daniel
Characterization of a Novel Dendritic Cell Population

Conventional DC (cDC) arise from circulating immediate precursors (pre-cDC), and are currently thought to be terminally differentiated. Here we show that cDC are capable of generating progeny that lost all characteristic features of cDC and aquired regulatory properties. Sorted bone marrow pre-cDCs were cultured on a stromal monolayer in the presence or absence of granulocyte-macrophage colony stimulating factor (GM-CSF). In the absence of GM-CSF, pre-cDC derived DCs gave rise to a homogeneous population of CD11clow MHClow cells (DC-regs) on day 8-10 of culture. DC-regs failed to up-regulate major histocompatibility complex class II (MHCII) and co-stimulatory molecules in response to DC maturation stimuli, were poor stimulators in T cell proliferation assays and suppressed T cell proliferation in cultures containing immuno-stimulatory DC. Co-transfer of DC-regs with DCs in vivo did not inhibit proliferation of T cells. These findings reveal the potential of DCs to generate a regulatory DC population with immunosuppressive properties.

Keywords: dendritic cell
Advisor: Cattral, Mark
Gorczynski, Reginald M.
Compensation for Wrongful Convictions: A Study towards an Effective Regime of Tort Liability

How would you feel if after having spent many years incarcerated for a crime that you did not commit and when finally you are released to a broken life where there is nobody to respond effectively to all the damages that you have and that you will continue to endure due to an unfortunate miscarriage of justice? In Canada, compensation for wrongful convictions is a legal issue which has yet to find a solution for those who the government has denied to pay compensation for and the damages such wrongful conviction brought to their lives. This thesis will analyze the legal problem of compensation for wrongful convictions in Canada from a tort law perspective and will present an alternative to the existing regime to serve justice to those who have been victims of miscarriages of justice.

Keywords: wrongful conviction
compensation
civil liability
public authorities
no-fault liability
fault liability
miscarriage of justice
Advisor: Yoon, Albert
A Topological Obstruction in a Control Problem

The reach control problem (RCP) characterizes a control design approach, based on computer science notions of object triangulation, that has been extensively developed as a means of guiding the complete transient response of a system, entirely within a desired polytopic region of state-space operation characterized by linear constraints on its states. This thesis expands upon results achieved in the area of RCP problem solvability under continuous feedback, identifying new necessary conditions. It accomplishes this using algebraic topology constructs, mapping the reach control problem to an equivalent topological one to successfully demonstrate conditions under which topological obstructions are generated. These obstructions, which render the RCP unsolvable by continuous feedback are then used to characterize equivalent conditions necessary for solvability of the problem. This thesis also serves to formally demonstrate the substantial advantages of the RCP design approach over more conventional industry techniques, by solving real-world problems with complex specifications.

Keywords: Control Systems
Complex Specifications
Advisor: Broucke, Mireille E.
Efficient Frame Transmission For Scalable Video Streaming with Dependency Structure

Efficient transmission schemes are presented for streaming scalable video over a link with limited capacity. The objective is to select a transmission sequence of data units and their transmission schedule such that the overall video quality is maximized. For video with a single spatial layer, optimal transmission schemes are obtained for two general classes of hierarchical prediction structures, which include the popular dyadic structure. Based on a new characterization of the interdependence among frames in terms of trees, structural properties of an optimal transmission schedule are derived. These properties lead to the development of a jointly optimal frame selection and scheduling algorithm, which has computational complexity that is quadratic in the number of frames. Then, using the concept of virtual deadlines, an efficient sub-optimal scheme for the transmission of video with multiple spatial layers is proposed. Simulation results show that the proposed schemes substantially outperform two existing alternatives.

Keywords: Video Streaming
Scheduling
Video transmission
frame selection
Virtual deadlines
display deadlines
Advisor: Liang, Ben
The Fortification of Salt with Iodine, Iron, and Folic Acid

Micronutrient poor diets around the globe and in particular in the developing world cause deficiencies in iron and folic acid. This may be rectified by the incorporation of these micronutrients into currently running salt iodization processes. The objective of this project was to develop folic acid and iodine spray solutions to be ready for pilot scale testing and to investigate the stability of triple fortified salt containing iodine, folic acid and microencapsulated ferrous fumarate. The optimal spray solutions were buffered to pH 9 with a carbonate/bicarbonate buffer to stabilize folic acid and contained 1%-2% w/v folic acid and 1%-3% w/v iodine (as KIO3). They remained in solution and retained ≥80% of both micronutrients after 5 months of storage at 25ºC and 45ºC. Double fortified salt produced using these spray solutions retained 100% of both folic acid and iodine over a 5 month period when stored at ambient conditions. Unfortunately triple fortified salt did not sufficiently retain the micronutrients due to excess moisture absorption and inadequate encapsulation of iron.

Keywords: salt
fortification
iodine
iron
folic acid
folate
micronutrient
Advisor: Diosady, Levente L.
Metabolic Responsiveness of a Novel GnRH-GFP Neuronal Cell Model

Gonadotropin-Releasing Hormone (GnRH), the master regulator of the reproductive axis, is hypothesized to play an essential role in directly sensing changes in energy levels to maintain fertility. There are a number of GnRH cell lines that have been utilized to study reproductive function; however these have been embryonic in origin and clonally derived. To investigate the cellular mechanisms underlying glucose responsiveness in GnRH neurons, we generated a novel GnRH cell line through immortalization, and fluorescent activated cell (FAC)-sorting of hypothalamic primary culture taken from a GnRH-GFP transgenic mouse. The mHypoA-GnRH/GFP neurons express a complement of markers of fully differentiated GnRH neurons. Glucose induces neuronal activation of mHypoA-GnRH/GFP neurons and glucose metabolism initiates an AMP-Protein Kinase (AMPK)-dependent mechanism to exert transcriptional and secretory control of GnRH. These findings support the use of this novel GnRH cell model for defining components involved in cellular and molecular action of glucose in GnRH neurons.

Keywords: Metabolism
Reproduction
Endocrinology
Hypothalamus
Advisor: Belsham, Denise
Effective Weld Properties for RHS-to-RHS Moment T-connections

An experimental program was developed to test various unreinforced RHS-to-RHS 90° T-connections subject to branch in-plane bending moment with the objective of determining the effectiveness of the welded joint. Twelve unique test specimens were designed to be weld-critical and the results from the full-scale tests revealed that the current equation for the effective elastic section modulus for in-plane bending, S_ip, given in Table K4.1 of ANSI/AISC 360 (2010) is conservative. A modification to the current requirements that limit the effective width of the transverse weld elements is proposed, resulting in a safe and more economical weld design method for RHS-to-RHS T-, Y- and X- connections subject to branch axial load or bending moment. It is also concluded that the fillet weld directional strength enhancement factor, (1.00 + 0.50sin1.5Ө), should not be used for strength calculations of welded joints to square and rectangular hollow structural sections.

Keywords: effective weld properties
rectangular hollow section
RHS-to-RHS moment T-connections
hollow structural section
Vierendeel truss
effective weld length
welding
robotic welding
automatic welding
gas metal arc welding
Advisor: Packer, Jeffrey A.
Investigating Supportive Care Needs of Parents of Children with Cancer: Is a Parent Support Group Intervention a Feasible Solution?

PURPOSE: (1) Investigate supportive care needs and preferences of parents of children with cancer (2) Assess the feasibility and preliminary outcomes of a Parent Support Group Intervention (PSGI). METHODS: One-hundred and eight parents completed the Parent Support Survey (PSS). Data were utilized in the creation of a PSGI. The PSGI consisted of eight 1.5-hour sessions encompassing guided group discussion. As part of feasibility, acceptability, recruitment/retention and preliminary outcomes were assessed. Parents completed outcome measures assessing knowledge of disease/treatment management, community/hospital/family resources, perception of social support and feelings of distress/anxiety. RESULTS: From the PSS, 86/108 (80%) of parents expressed interest in a PSGI. Fourteen parents attended the PSGI only once and 14 parents attended the PSGI more than once. Outcome measure results depict favourable changes after group attendance. Recruitment and retention data highlight challenges. CONCLUSIONS: Information gained from this study will be used to improve planning and delievery of future PSGI’s.

Keywords: childhood cancer
parents of children having cancer
supportive care needs of parents of children having cancer
parent support groups
Advisor: Barrera, Maru
Cost-effectiveness of Intermittent versus Continuous Androgen Deprivation Therapy in Advanced Prostate Cancer

Background: Androgen deprivation therapy (ADT) has known adverse effects (AEs). Intermittent (INT) ADT may reduce AEs, improve quality of life, and lower costs compared to continuous (CONT) treatment. Objective: To evaluate the cost-effectiveness of INT vs CONT ADT in men with advanced prostate cancer. Methods: A lifetime Markov individual simulation model was developed to evaluate the incremental cost per quality adjusted life month (QALM) of INT vs CONT ADT. Results: INT dominated CONT ADT (mean total costs $94,460 vs $109,431; mean total QALMs 47.0 vs 46.4). INT ADT resulted in less time on therapy (22.4 vs 56.8 months), fewer hip fractures (0.080 vs 0.093 per patient), and fewer total cases of sexual dysfunction (72.5% vs 87.0% of patients) and cardiovascular disease (38.7% vs 44.6% of patients). Conclusions: These results suggest INT ADT is cost-effective compared to CONT ADT however, differences were small. Additional research is required to confirm these findings.

Keywords: Economic evaluation
cost-effectiveness
prostate cancer
Advisor: Krahn, Murray
Unlocking the Competitiveness of the Fee: Is Canadian Mobile Service Providers Charging a Fee to Remove the Software Lock after the Contract Expires Anti-competitive?

Most mobile phones in Canada contain software that prevents the consumer from using the phone on multiple networks. This is known as a ‘software lock’, and is installed prior to the point of sale by the mobile service providers. As of 2011, all three large Canadian service providers have adopted a similar practice to charge a fee to remove the software lock from phones. This fee applies even after the consumer’s service contract with the provider ends. This thesis examines whether the practice of charging consumer a fee for removing the software lock after the contract expires is anti-competitive in Canada. Through examining economic theory and undergoing legal analysis, this thesis will argue that while the practice is clearly anti-competitive, it does not substantially lessen competition enough to be prevented under competition law. This thesis suggests that this industry practice should be prohibited through the passing of consumer protection legislation.

Keywords: competition
software Locking
mobile phones
Advisor: Iacobucci, Edward
Role of Notch Signaling Network in Gene Expression Patterns of Angiogenic EC in 3D Matrix and 2D Confluent Monolayer

This study examined the differential gene expression patterns between endothelial cells (EC) from 2D monolayer and EC from angiogenic capillary-like network in 3D matrices. Our microarray analysis comparing 3D to 2D EC cultures detected upregulation of 854 protein-coding genes and downregulation of 863 genes. We show that Notch signaling pathway is highly regulated in angiogenesis, induced by change in ECM dimension. Notch target genes Hey1, HeyL, Hes1 and Hes4 transcription factors were upregulated in 3D angiogenic EC, which were confirmed with qRT-PCR. Moreover, we are the first to report enrichment of FoxS1 transcription factor mRNA during angiogenesis in 3D ECM. Next, we asked whether epigenetic mechanisms partly mediate cis-trans response in angiogenesis. Our sodium bisulfite sequencing analyses did not indicate a role for DNA methylation in the expression of key Notch signaling components. However, our pilot studies indicate a potential role for lncRNAs in controlling EC phenotype in angiogenic response.

Keywords: Notch
endothelial gene expression
Advisor: Marsden, Philip A.
Design of an Approach to Characterize CD11b Cre x LoxP BDNF Deletion in Mice: Implications for Neuropathic Pain

Background: An approach designed to characterize BDNF gene deletion within microglia of the dorsal horn of the spinal cord does not currently exist. Therefore, my goal was to develop methods to assess Cre- mediated BDNF deletion. To this end I designed and tested two different approaches focusing on the aspects of BDNF mRNA expression or genomic level gene deletion. Methods: Approach 1: BDNF messenger RNA was detected by in situ hybridization. Approach 2: BDNF gene deletion was detected by a positive signal semi-quantitative Polymerase Chain Reaction (PCR). Results: In situ hybridization detected spinal BDNF and regional changes in BDNF mRNA following PNI in wild-type mice. The BDNF PCR detected Cre-mediated BDNF deletions in transgenic animals. Conclusion: Two approaches have been developed and initial tests of these approaches show promising results and will provide valuable tools for researchers investigating BDNF deletion in transgenic animals.

Keywords: BDNF
Neuropathic Pain
Advisor: Salter, Michael
Cost-effectiveness of Intravenous Antibiotics in Moderate to Severe Diabetic Foot Infections and Efficacy as a Function of Resistance Rates in the Case of Methicillan-resistant Staphylococcus Aureus in Diabetic Foot Infections

Objectives: The objectives of the research were to determine which intravenous (IV) antibiotics were cost-effective in Diabetic Foot Infections (DFIs) and to assess the impact of MRSA prevalence on clinical outcome. Methods: A Cost-effectiveness analysis (CEA) was performed on IV antibiotics used to treat moderate to severe DFIs in hospitalized patients. MRSA prevalence was taken into account by calculating an ‘Adjusted cure rate’ and re-analysing the CEA. Results: In the original CEA, imipenem/cilastatin was the cost-effective agent. When MRSA prevalence was taken into account imipenem/cilastatin, moxifloxacin, cefoxitin and ertapenem were cost-effective antibiotics. Conclusion: MRSA prevalence adjustments changed the results of the CEA and included classes of IV antibiotics that are seen being using in practice, such as fluoroquinolones and cephalosporins. These methods could potentially have an impact on the evaluation of clinical cure rates and resistance when evaluating the literature.

Keywords: Cost-effectiveness analysis
Diabetic foot infection
Antibiotics
Advisor: Einarson, Thomas Ray
Compressed Sensing for Jointly Sparse Signals

Compressed sensing is an emerging field, which proposes that a small collection of linear projections of a sparse signal contains enough information for perfect reconstruction of the signal. In this thesis, we study the general problem of modeling and reconstructing spatially or temporally correlated sparse signals in a distributed scenario. The correlation among signals provides an additional information, which could be captured by joint sparsity models. After modeling the correlation, we propose two different reconstruction algorithms that are able to successfully exploit this additional information. The first algorithm is a very fast greedy algorithm, which is suitable for large scale problems and can exploit spatial correlation. The second algorithm is based on a thresholding algorithm and can exploit both the temporal and spatial correlation. We also generalize the standard joint sparsity model and propose a new model for capturing the correlation in the sensor networks.

Keywords: Compressed Sensing
Advisor: Valaee, Shahrokh