VictorJuiz/thesis huggingface dataset:
Is Severe Early Childhood Caries Associated with Dental Caries in Adulthood?

Dental caries in the primary dentition is a risk factor for dental caries in the permanent dentition. The aim of this pilot project was to establish if high levels of dental caries very early in life translate into increased levels of dental caries in adulthood. Our objective was to determine the mean DMFT score of individuals with a history of severe early childhood caries (S-ECC) and compare it with the mean DMFT score of individuals who were caries-free in the primary dentition. The results of our analyses indicate that adults who experienced S-ECC manifest significantly higher levels of dental caries in their adulthood. The best predictor for dental caries in adulthood was caries experience in primary dentition. This preliminary study, the first of its kind, suggests that early childhood caries might indeed be associated with dental caries in adulthood.

Keywords: childhood caries
risk factor
adultdhood
disease trajectory
Advisor: Kulkarni, Gajanan
Kulkarni, Gajanan
Tungsten Doped Tantalum Oxide Anodes for Electrochemical Disinfection of Wastewater

Tungsten doped tantalum oxide films on titanium substrates were investigated for use as anodes in the electrochemical disinfection of wastewater (measured through e. coli inactivation). A sol-gel method for fabricating these films was developed that allowed for control of both the doping concentration (through volumes of tantalum and tungsten ethoxide added to the solutions), and the thickness (through the number of layers applied). The morphology and composition of these films were investigated using SEM and EDX mapping; the morphology was found to be connected to the fabrication heating procedure. Three different doping concentrations (0%, 8% and 14% tungsten by volume of added metals) were investigated for: 1) electrochemical activity; 2) long-term stability; and 3) disinfection capabilities. The 14% samples demonstrated the highest conductivity (0.06μS/cm), good long-term stability (verified using ICPMS, SEM and EDX analysis) and the best electrochemical activity for removal of e. coli (based on wastewater tests).

Keywords: Electrochemical disinfection
Doped metal oxide
Advisor: Thorpe, Steven J.
Kirk, Donald W.
Investigation of the Production, Distribution, and Trafficking of MMP-9 in Classically Activated Macrophages

As major effector cells of the innate immune response, macrophages must adeptly migrate from blood to infected tissues. Endothelial transmigration is accomplished by matrix metalloproteinase (MMP)-induced degradation of basement membrane and extracellular matrix components. The classical activation of macrophages with LPS and IFN-γ causes enhanced microtubule stabilization and secretion of MMPs. Macrophages upregulate MMP-9 expression and secretion upon immunological challenge, and require its activity for migration during inflammatory response. However, the dynamics of MMP-9 production and intracellular distribution, as well as the mechanisms responsible for its trafficking, are unknown. Using immunofluorescent imaging, we localized intracellular MMP-9 to small Golgi-derived cytoplasmic vesicles that contain calreticulin and PDI, in activated macrophages. Vesicular organelles of MMP-9 aligned along stable subsets of microtubules and colocalized with the anterograde molecular motor protein, kinesin. We demonstrated a functional contribution of stable MTs in the enhanced trafficking of MMP-9 extracellularly, and showed that heterogeneity exists in macrophage cell populations with respect to MMP-9 production.

Keywords: macrophages
MMP-9
microtubules
Macrophage activation
Matrix Metalloproteinase 9
Trafficking
Advisor: Harrison, Rene E.
What does a Single-item Measure of Self-rated Mental Health tell us? Systematic Review of Literature and Analysis of the Canadian Community Health Survey

A single-item measure of self-rated mental health (SRMH) asks respondents to rate their mental health on a 5-point scale from ‘excellent’ to ‘poor’. SRMH is being used increasingly in research and on population health surveys. However, little is known about this item, as there are no literature reviews and few formal validation studies. The aim of this study is to understand what SRMH measures by conducting the first known systematic review of SRMH literature, followed by analysis of the Canadian Community Health Survey (CCHS 1.2). Results of the systematic review reveal SRMH has relationships with mental health scales, mental disorders, self-rated health, health problems, service utilization, and service satisfaction. Analysis of CCHS 1.2 data finds SRMH is associated with psychiatric diagnoses, distress, physical health, and sociodemographic characteristics. Both studies conclude SRMH is measuring mental health and more; however, there needs to be more research to understand the specifics of these relationships.

Keywords: self rated mental health
systematic review
Canadian Community Health Survey
Advisor: Bierman, Arlene
The FIIND Domain of Nlrp1b Promotes Oligomerization and Pro-caspase-1 Activation in Response to Lethal Toxin of Bacillus anthracis

Lethal toxin (LeTx) of Bacillus anthracis kills murine macrophages in a caspase-1 and Nod-like-receptor-protein 1b (Nlrp1b)-dependent manner. Nlrp1b detects intoxication, and self-associates to form a macromolecular complex called the inflammasome, which activates the pro-caspase-1 zymogen. I heterologously reconstituted the Nlrp1b inflammasome in human fibroblasts to characterize the role of the FIIND domain of Nlrp1b in pro-caspase-1 activation. Amino-terminal truncation analysis of Nlrp1b revealed that Nlrp1b1100-1233, containing the CARD domain and amino-terminal 42 amino acids within the FIIND domain was the minimal region that self-associated and activated pro-caspase-1. Residues 1100EIKLQIK1106 within the FIIND domain were critical for self-association and pro-caspase-1 activation potential of Nlrp1b1100-1233, but not for binding to pro-caspase-1. Furthermore, residues 1100EIKLQIK1106 were critical for cell death and pro-caspase-1 activation potential of full-length Nlrp1b upon intoxication. These data suggest that after Nlrp1b senses intoxication, the FIIND domain promotes self-association of Nlrp1b, which activates pro-caspase-1 zymogen due to induced pro-caspase-1 proximity.

Keywords: Nlrp1b
FIIND domain
anthrax
lethal toxin
caspase-1
inflammasome
anthracis
pyroptosis
Advisor: Mogridge, Jeremy
Raspberries and Human Health: A Clinical Perspective on the Bioactivity and Bioavailability of Red Raspberry Antioxidants

Red raspberries, as an excellent source of dietary antioxidants, were investigated for their effect on oxidative stress in healthy adults. Study 1 measured effects of chronic exposure in a parallel, multi-dose intervention. Subjects consumed one-cup red raspberries (1cR) daily for two-weeks, then were randomized to consume 1cR, 2cR or 4cR for additional two-weeks (n=8, by group). There was a reduction in TBARS, indicating a decrease in lipid peroxidation, after two-weeks of intervention in the 1cR group, but effects were not significant at week 4, or for other treatment groups. Study 2 measured effects of acute exposure using a cross-over design. Subjects (n=8) consumed single treatments of 1cR, 2cR, 4cR, bread and bread plus vitamin C. Post-prandial oxidative stress responses were complex and appeared related to calorie and antioxidant load. Overall there was no clear relationship between red raspberry consumption and protection against oxidative stress.

Keywords: raspberry
antioxidants
oxidative stress
human intervention
Advisor: Rao, A. Venketeshwer
Pakistani Immigrant Parental Perspectives on New Media Literacies

This phenomenological study researches Pakistani immigrant parents’ perspectives and attitudes on how their children use new media technologies. Parental attitudes are directly linked to student achievement (Hampton, Mumford & Bond, 1998) and parents are the gatekeepers of technology use in the home, where, during unstructured time, children can experiment and develop skills in using new technologies (Ito et al, 2010). Therefore, this study looks at how parents, through their actions and attitudes, encourage or discourage their children from developing competencies in using new media technologies By examining the pedagogical histories of parents and their concerns and rules about technology use, this study adds to the literature on parental attitudes towards the use of technology as a pedagogical tool. Further, this study examines the issue from an immigrant perspective, focusing on 10 Pakistani immigrant parents who live in the Greater Toronto Area and whose children attend pubic school.

Keywords: parental attitudes
parental perceptions
new media literacies
multimodality
immigrant
immigrant education
technology
new media technology
Pakistan
competency
Advisor: McCready, Lance T.
Effect of Folate Deficiency on the Sensitivity of Colon Cancer Cells to 5-Fluorouracil Based Chemotherapy

Folate is an essential cofactor in one-carbon transfer reactions including nucleotide biosynthesis, thereby playing an important role in DNA replication and repair. In cancer cells, folate depletion interrupts DNA synthesis, thereby causing cancer cell death. This has been the basis for chemotherapy using antifolates and 5-fluorouracil (5FU). We determined the effect of folate deficiency on the sensitivity of colon cancer cells to 5FU in a well established in vitro model of folate deficiency. Folate deficient cells had lower intracellular folate concentrations, had functional evidence of intracellular folate depletion, proliferated less, and had increased chemosensitivity to 5FU with and without Leucovorin. These data suggest that folate deficiency significantly enhances the sensitivity of colon cancer cells to 5FU based chemotherapy via changes in intracellular folate. Dietary or other strategies to deplete intracellular folate concentrations in colon cancer cells to enhance chemosensitivity to 5FU are worthy of further investigation.

Keywords: Folate
Colon Cancer
Fluorouracil
Chemotherapy
Advisor: Kim, Young-In
R.A.G.E.: Reflections on Acts of Gendered Violence and our Educational Lives

This is an arts-informed qualitative research study looking at violence against women and how it affects their educational outcomes. It uses an art installation in which the narratives of the women involved are combined with photographs and real world objects in which viewers take on a ‘walking meditation’ as well as the use of participation stations for viewer feedback and further sharing of stories anonymously. This project is based on the belief that through a feminist research lens, participatory practice with the use of storytelling can be a form of transformation in community development.

Keywords: arts-informed
qualitative research study
violence against women
educational outcomes
art installation
feminist research lens
participatory practice
storytelling
transformation
community development
Advisor: Restoule, Jean-Paul
Mental Health, Identity and Coping Following Hate Crime Victimization

Previous research has explored the impact on individuals following hate crime victimization, including prevalence estimates in the lesbian, gay, bisexual, transgender/transsexual (LGBT) community. Past research, however, has been limited in its scope when exploring the impact of the hate crime event on the overall mental health of the survivor often examining only one measure of functioning. The current project aims to fill this gap by analyzing measures of posttraumatic symptoms, depression, stress and dissociation, collected on a sample of 30 LGBT individuals who have experienced hate crime victimization. In addition to clinical features, this study also analysed identity, openness about LGBT identity and coping behaviours. Results indicated that social support, identity and coping impact recovery from hate crime victimization. The effect of these factors differed over time (within one month of the hate crime) and up to as much as five years later (within the month of the interview).

Keywords: Mental Health
Hate Crimes
LGBT
Victimization
Advisor: Gillis, Joseph Roy
Moving Beyond Cultural Inclusion Towards a Curriculum of Settler Colonial Responsibility: A Teacher Education Curriculum Analysis

Critical Indigenous scholars and their explicit allies have emphasized the need for curriculum and pedagogy in teacher education to address settler colonialism in Canada (Cannon, forthcoming(a); Cannon and Sunseri, 2011; Dion, 2009; Friedel, 2010a; Haig-Brown, 2009; Schick, 2010; Schick and St. Denis 2003, 2005; & St. Denis, 2007) . This thesis is primarily concerned with the existence of and possibilities for such a curriculum. In this thesis, I analyzed the curricula used in the three required courses of the secondary consecutive Initial Teacher Education (ITE) program in the 2011-2012 year at OISE for representations of settler colonialism in Canada. This study finds that while the curriculum in the ITE program at OISE focuses broadly on social justice, it shies away from addressing the ways that Canadians are complicit in ongoing colonialism. The thesis ends by highlighting some clear possibilities and challenges for a curriculum of settler colonial responsibility.

Keywords: Teacher Education
Settler Colonialism
Racism
Indigenous
Aboriginal
Multiculturalism
Whiteness Studies
Advisor: Cannon, Martin
Cumulative Contextual Risk, Maternal Responsivity, and Social Cognition at 18 Months

By 18 months children demonstrate a range of social-cognitive skills that reflect their emerging capacity to understand and engage in intentional relations with others. Intention understanding is a critical component of children’s social cognition at this age. Although individual differences in social cognition have been linked to neurocognitive maturation, socio-cultural models of development suggest that environmental influences operate in the development of intention understanding, with distal factors operating through proximal processes. In the current study of 501 children and their mothers, we tested and found support for a model in which an accumulation of distal environmental risks was associated with lower maternal responsivity, which was in turn associated with lower social-cognitive competency at 18 months. In addition, part of this effect operated through children’s concurrent language ability. Findings are discussed with respect to the Vygotskian themes of internalization and semiotic mediation.

Keywords: social cognition
social disadvantage
parenting
cumulative risk
Advisor: Jenkins, Jennifer M.
Identity Reformulation among Young Women with Breast Cancer

The purpose of this study was threefold; to examine the unique challenges faced among young breast cancer survivors’ self-concepts; to explore the identity reformulation process as they adjust to a new lifestyle after completing medical treatment; and to identify women’s creative problem solving solution used to mitigate any long-term distress and discontinuity between past, current and ideal selves. In-person semi-structured interviews were conducted with 10 young breast cancer survivors (aged 32-45). The results indicated that exploring women’s narratives through the identity reformulation process created an alternative approach to the four prescribed quality of life domains used to understand survivors’ overall wellbeing. The results also identified three shared domains of social location (gender, youth and health status) that intersected in women’s identity reformulation process. These findings have implications for psychosocial oncology literature, as well as clinical practice for mental health practitioners. Limitations and recommendations for areas of future research were discussed.

Keywords: young women
breast cancer survivors
psychosocial wellbeing
Advisor: Piran, Niva
Governing Through Competency: Race, Pathologization, and the Limits of Mental Health Outreach

This thesis examines how cultural competency operates as a regime of governmentality. Inspired by Foucauldian genealogy, institutional ethnography, and Said’s concept of contrapuntality, this thesis problematizes the seamless production of racialized bodies in relation to mental disorder. I begin by elaborating a theoretical framework for interpreting race and madness as mutually constructed ordering practices. I then analyze what cultural competence produces and sustains in a position paper published by the Ontario Federation of Community Mental Health and Addiction Programs. I argue the Federation dismisses ongoing institutional violence—suggesting it is simply the perception, as opposed to the everyday reality, of discrimination that causes problems such as low educational attainment among youth of colour. To further support this claim, I deconstruct narratives of low self-esteem, maladaptive coping, depression, and denial of mental illness in the community needs assessments of two of the Federation’s member organizations: Hong Fook and Across Boundaries.

Keywords: construction of mental disorder
racial governmentality
immigrant women
minority youth
cultural competency
cross-cultural mental health
Brief Symptom Inventory
Attitudes Towards Seeking Professional Psychological Help Scale
community needs assessment
Postcolonial Studies
institutional ethnography
Disability Studies
race
deviance
depression
self-esteem
madness
oppression as disease
anti-racism
health equity
antipsychiatry
feminist
Foucauldian genealogy
contrapuntal reading
psychiatric survivors
multicultural practice
technologies of the self
social organization of mental health
Advisor: Razack, Sherene
Liberian Youth Speak: Life Histories of Young Former Refugees and their Interactions with the Canadian School System

This study explores the interactions of four Liberian youth with the public education system in Ontario since their arrival as refugees. Using life histories developed with each participant, I have sought to understand and engage with the ways that these students negotiate their social and personal identities within the context of the majority discourses and practices of education in Ontario. By foregrounding the experiences and voices of the participants, it becomes possible to critically analyze the power relations that exist both to limit and empower these youth as they navigate their social and educational contexts. It becomes clear in the life histories that society’s dominant discourses of normalcy work to ignore or make irrelevant the complex identities that these youth inhabit and exhibit in their daily lives. However, they creatively exercise their individual agency to take advantage of the tremendous opportunities they feel are available to them in Canada.

Keywords: Liberian refugee youth
Identity and schooling
Advisor: Gérin-Lajoie, Diane
Differences in Reading Strategies and Differential Item Functioning on PCAP 2007 Reading Assessment

Pan-Canadian Assessment Program (PCAP) 2007 reading ability item data and contextual data on reading strategies were analyzed to investigate the relationship between self-reported reading strategies and item difficulty. Students who reported using higher- or lower-order strategies were identified through a factor analysis. The purpose of this study was to investigate whether students with the same underlying reading ability but who reported using different reading strategies found the items differentially difficult. Differential item functioning (DIF) analyses identified the items on which students who tended to use higher-order reading strategies excelled, which were selected response items, but students who preferred using lower-order strategies found these items more difficult. The opposite pattern was found for constructed response items. The results of the study suggest that DIF analyses can be used to investigate which reading strategies are related to item difficulty when controlling for students’ level of ability.

Keywords: differential item functioning
reading strategies
higher-order reading strategies
lower-order reading strategies
Advisor: Childs, Ruth A.
Blood Filtration for Multiplexed Point-of-care Diagnostic Devices

In the developing world, there are large populations suffering from infectious diseases, many of whom are located in remote regions. With the rapid growth in microfluidic systems in recent years, complex functions of conventional diagnostic equipment have been miniaturized and integrated into small devices at the size of a credit card (so-called portable Point-of-care (POC) devices). In this thesis a novel approach to overcoming the challenge of in-field biological sample processing and preparation to produce high quality fluids that can be readily used for downstream testings is described and proof of concept experiments presented. This approach uses hydrodynamic effects and combines nanoporous membrane with microfluidic systems and to filter the cellular component of blood. Experiments presented here demonstrate successful cells filtration from whole blood. Employing hydrodynamic effects is also shown to be an effective and potentially useful technique to isolate cells and plasma within appropriate micro-architectures.

Keywords: microfluidics, point of care
blood filtration
Advisor: Sinton, David
The Paradox of Socially Organized Nursing Care Work

As contemporary health care organizations struggle to control costs, yet deliver quality patient-centred care, the concept of care becomes socially transformed through the use of quality improvement models (i.e., Lean methodology) and quality assurance documentation. This research investigates how nurses’ care work is socially organized in a system that defines care through quality management practices. I use Dorothy E. Smith’s Institutional Ethnography as a feminist mode of inquiry and as a guiding framework for my interviews with nurse participants as I explore the complex social relations within the health care system from the vantage point of nurses undertaking care work. I argue that the social reorganization of care work has affected the emotional lives of nurses as they try to balance actual patient-centred care with their reporting obligations under quality management.

Keywords: institutional ethnography
care work
nurses
emotional labour
adult education
organizational development
Advisor: Jackson, Nancy
The Development of a Thin Slice Methodology for Coding Scaffolding between Siblings

The goal of the present study was to develop and compare two different methods for rating scaffolding between siblings: a thin slice approach and an interval coding approach. Fifty younger (age=3 years) and 50 older (age 3-7 years) siblings interacted for five minutes on a cooperation task and scaffolding during the task was coded for each child. Internal consistency was excellent for the thin slice measure and questionable for the interval measure. Inter-rater reliability was good for both. Thin-slicing was more strongly related to predicted variables (children’s theory of mind, language, age, cooperation, positive and negative behavior) than interval coding, and reduces demands on resources in terms of training and reliability. The development of a reliable and valid measurement for the assessment of child-to-child scaffolding, which involves limited training and is quick to code, will be a useful research and practice tool for developing children’s cooperation skills in applied settings.

Keywords: Siblings
Methodology
Scaffolding
Thin Slice
Advisor: Jenkins, Jennifer M.