VictorJuiz/thesis huggingface dataset:
Determinants of Adaptation and their Interactions in Experimental Populations of Saccharomyces cerevisiae
Keywords: adaptation
evolution
yeast
cerevisiae
speciation

The goal of my M.Sc. thesis research was to investigate the link between divergent adaptation and reproductive isolation in speciation, the process by which one species becomes two over time. Previous studies followed the evolution of 12 replicate experimental populations of the yeast Saccharomyces cerevisiae in either of two non-optimal environments, high-salt or low-glucose, over 500 generations. In this research, I re-sequenced the whole genomes of six diploid strains of these populations, three from high salt and three from low glucose. Among the replicates, there was remarkable degree of parallelism in the underlying determinants of adaptation including multiple mutations in PMA1, encoding the main proton exporter, in high-salt and in several genes in the ras signalling pathway in both environments. A variety of positive and negative epistatic interactions were found among mutations from within and among populations, emphasizing the importance of epistasis in adaptation and potentially in speciation.
Finding Meaning in Place: The Perspectives of People with Severe Mental Illness Living Long Term in a Psychiatric Hospital
Keywords: place
severe mental illness

This study explored the perspectives on place of eight people with severe mental illness living, for one year or longer, in an urban psychiatric hospital. The research questions were: how do people with severe mental illness view the psychiatric hospital as place?; and how do they make meaning of the experience of living in hospital. The research employed a phenomenological approach, as described by Giorgi (1985). Using purposive sampling, one time, semi-structured, individual interviews were conducted. The audio recorded interviews were transcribed and thematically coded using Giorgi’s (2005) method. The meanings of the participants’ experiences are captured by the meta-theme: this is not a home; it’s a hospital. Four additional major themes emerged; and each of the major themes also had sub-themes The findings of this study challenged commonly held assumptions on how people living long term in a psychiatric hospital view the hospital as place and on institutionalization.
Chemical Genetic Interactions for Antibiotics in Escherichia coli
Keywords: Escherichia coli
chemical genetics

The discovery of penicillin ushered in the era of the mass use of antibiotics in clinical settings. Today the development of antibiotic resistance and lack of discoveries of new antibiotics have created a serious public health concern. Recently, new experimental tools, such as bacterial genome-wide deletion collections, have provided exciting new possibilities for studying biological networks in bacteria that could potentially also be exploited for antibiotic research. In this study, I used the Keio knockout collection of Escherichia coli (E.coli) strains, along with an in-house collection of hypomorphic alleles of essential genes, to study the effects of chemical perturbations by twenty-two antibiotics and four other chemicals on the biological pathways of E.coli. These experiments uncovered a set of mutants hypersensitive to drugs of different classes, information which could potentially be exploited for future antibiotic research. The results also shed light on how different classes of antibiotics behave with respect to their target pathways and the various functional modules with which they are associated.
Understanding the Mechanism of Aberrant FLVCR1 Splicing and Disrupted erythropoiesis in Diamond-Blackfan Anemia
Keywords: Diamond-Blackfan Anemia
FLVCR1 aberrant splicing
RPS19
Erythropoiesis
SR proteins
Tra2Beta

Diamond Blackfan Anemia (DBA) is a congenital disorder characterized by a specific reduction in erythroid progenitor cells. Approximately 55% of patients have heterozygous mutations in ribosomal protein with 25% of these mutations in RPS19. However, it is unclear how a defect in ribosomal proteins specifically disrupts erythroid development. FLVCR1, a heme exporter, has been implicated as a potential DBA factor. FLVCR1 is essential for erythropoiesis as its disruption leads to apoptosis and disrupted erythroid differentiation. Though no FLVCR1 mutations have been found in DBA patients, our lab has shown that it is aberrantly spliced in DBA erythroid cells. Using RPS19 reduced K562 erythroid cells, I found that disruption of RPS19 leads to aberrant FLVCR1 splicing, disrupted erythropoiesis and reduced Tra2-β, ASF2 and SRp30c protein expression. This was specific to DBA as I did not find these features in a cell culture model of Shwachmann Diamond Syndrome, another ribosomal disorder.
Towards International Islamic Human Rights: A Comparative Study of Islamic Law, Shari’ah, with Universal Human Rights as Defined in the International Bill of Human Rights
Keywords: International Islamic Human Rights

Islamic law, also known as Shari’ah law, is one of the most complex and multifaceted, yet easily misunderstood areas of law. It is complex because its subjects, but not limited to, human rights, politics, religion, economics, and criminal. Islamic law is also misunderstood because it is greatly understudied and grossly over-generalized. This regretfully has lead to a situation where Islamic law is often characterized as an inhumane and discriminatory set of laws which have no respect for human rights. The questions that remain to be answered are whether or not there is a human rights discourse in Islamic law, and is Islamic law compatible with the Universal Declaration of Human Rights and its two subsequent International Covenants? The aim of this paper is to ascertain whether or not Shari’ah law, as it has been reflected in the Islamic human rights documents discussed, is compatible with universal human rights standards.
Mindfulness beyond the Third Wave: The Role of Mindfulness outside the Cognitive-behavioural Tradition
Keywords: mindfulness
psychotherapy
Buddhism
psychotherapeutic modality
psychology
qualitative research
therapist

Mindfulness has been defined in cognitive-behavioural terms in the mental health literature despite its broader application by many practitioners. Mindfulness is a complex and often ambiguous concept that has historically been understood and applied in myriad ways depending on the context; thus its application to psychotherapy outside the cognitive-behavioural tradition is not necessarily straightforward, and has not been addressed. This study addressed this gap in the literature through interviews with 9 experienced psychotherapists who integrate mindfulness with non-cognitive-behavioural psychotherapeutic modalities or eclectic psychotherapy. Interviews addressed how participants a) define mindfulness, b) apply it to psychotherapy, and c) the aims of that integration. Data were analyzed via the grounded theory approach. The results depicted mindfulness and its potential applications as broader than its presentation in the mental health literature, and emphasized the role of contexts in shaping conceptualization and application. A broad, context-based model of mindfulness/psychotherapy integration is proposed.
Composition Skills of Children with ADHD: An Intervention Study to Improve Communicative Clarity
Keywords: ADHD
Communication

The aim of the current study was to assess whether providing visual feedback on the written compositions of children with ADHD was an effective method to improve communicative clarity. Participants were twenty children, aged 7 to 9 years with ADHD. Children composed instructions on how to build a figure so that a confederate could reproduce the image based only on the child’s instructions. Participants in the experimental group received feedback on their compositions in the form of the reconstructed figure and were able to then compare the figures side by side on-screen and edit their instructions. Participants who received visual feedback outperformed participants in the no feedback group on the clarity of compositions at post-test, and at a six-week delayed post-test, and were able to apply their learning to transfer tasks. This research makes valuable contributions to understanding the factors that contribute to successful early writing for children with ADHD.
An Examination of Trauma-mediated Pathways from Childhood Maltreatment to Alcohol and Marijuana Use and the Perpetration of Dating Violence in Adolescence
Keywords: Childhood Maltreatment
Substance Use
Dating Violence
Posttraumatic Stress

Despite high rates of alcohol and marijuana use and dating violence among individuals between the ages of 15 and 24, limited research has examined a link between substance use and dating violence within adolescent samples. Moreover, although both problem drinking and dating violence perpetration have been linked to a history of childhood maltreatment (CM), few studies have examined the potential mechanisms of a relationship between these variables. The current study tested the predictive role of CM in adolescent alcohol and cannabis use and dating violence perpetration in a sample of youth with CM histories; in particular, we were interested in predicting the temporal co-occurrence of these behaviours. It was hypothesized that trauma symptomatology would play a mediating role in this relationship. Results demonstrated that only witnessing emotional domestic violence predicted the co-occurrence of substance use and dating violence; in general, trauma symptomatology was not found to be a significant mediator.
Relationship between Participation in the Webinar and Students’ Behaviours and Engagement in Online Learning
Keywords: Online learning
Behaviours
Engagement
Webinar

A graduate level Flex Mode course “Introduction to Computers in Education” using online learning concept, which includes the discourse environment and the webinars, was investigated in the current study. Twenty one students enrolled in the course in fall 2010. Since the design of this course is new and in its experimental stage, this study explored how webinar participation predicts students’ behaviours and engagement in online learning, and the experiences and perspectives of students who take this course. Data retrieved from the course database show that webinar participation correlated significantly with the engagement construct (about reading and writing online). Results from the questionnaire suggest that students were satisfied with both the webinar and online discussion components of the course. Interview findings indicate that students credit the webinar with helping them feel more connected to their classmates. Implications for the design of online learning program will be discussed.
Bodied Curriculum: A Rhizomean Landscape of Possibility
Keywords: community
currere
ecology
elementary education
embodiment
ethics
experiential learning
garden
holistic
lived experience
narrative inquiry
relationality

Undergoing a self-study using the method of currere (Pinar, 1976), I examine my own learning as holistic, embodied, and relational in the context of my mother’s garden. Specifically, I explore my mother’s garden as a site of relational learning that intersects with various classrooms that feature in my educational experiences. The garden and the classroom intersect with/in one curricular landscape, where self and other engage in an embodied process fostering connections and knowledges about each other and place. In bringing forth my narrative through currere, I engage in reflective and reflexive praxis through journal writing, poetry, meditation, and photographic collage. Using these forms of expression, I reflect upon my experiential learning process, analyze issues and concepts related to the body-in-movement, as well as focus on community connections and ecology-based learning as pedagogical praxis.
The Results and Implications of Fundraising in Elementary Public Schools: Interviews with Ontario Principals
Keywords: fundraising strategies
elementary schools
principals
equity
neoliberalism
commercialism
students
exclusions
dignity
Ministry of Education
fundraising results
fundraising disparities
achievement
democracy
curriculum
funding
public schools
funding cuts

A growing pressure to fundraise at the school level in Ontario is a direct result of funding cuts by the Ministry of Education. The purpose of this study is to examine and analyze the kinds of different fundraising strategies principals use in select elementary public schools in a southern Ontario large city and to determine the positive and negative results of fundraising. Principals of eight elementary schools were interviewed from high and low fundraising schools. Five included school observation tours. The findings show a positive correlation between student achievement and fundraised amounts and utilization, but that the benefits of fundraising only apply to a small number of students. The findings also show that an equal educational opportunity is not being provided to all students, and that moral, civic and democratic values are being eroded. This study highlights the need for Ontario’s Ministry of Education to fully fund public education.
An Examination of the Role of Parental Influences on Girl’s Development of Embodiment
Keywords: sociocultural factors
parental role
body image
embodiment
protective factors
risk factors
adolescent development
social location

Adolescence is marked with significant changes in how girls feel and act within their bodies, and is considered a special risk period for body image disruptions. Cross sectional quantitative research within this area suggests that parents represent an important contextual and developmental contributor to body image. The present study aimed to address gaps in previous research by investigating parental influences, including both protective and risk factors, on girls’ embodied experiences through utilizing a prospective qualitative design with a diverse sample of twelve girls, ages 9-18, interviewed annually over four years. Results revealed the presence of both protective and risk factors related to embodiment experiences within the parental relationships, including aspects of relational qualities, self-care, evaluative gaze and social location. Results are discussed in relation to Bronfenbrenner’s Ecological Systems Theory and to previous research. The implications for future research are also discussed.
Canadian Youth Abroad: Rethinking Issues of Power and Privilege
Keywords: Youth
International Development
Volunteerism
Equity

Since the 1960s, over 65,000 young Canadians have participated in volunteer abroad programs (Tiessen, 2008). Lately, the media and academia have questioned and criticized the benefits of volunteerism as development. This study highlights how issues of power and privilege extend beyond the individual, and reaches into institutional structures. The research design uses Institutional Ethnography (IE) as a method of inquiry, and maps out the social relations between the experiences of seven former youth volunteers and field staff, and their organizations. The aim is to explore how to improve individual and organizational pedagogy in the field of international volunteering, so that equity becomes a commitment by everyone in the development of sustainable and just communities.
A Microfluidic Platform for the Automated Multimodal Assessment of Small Artery Structure and Function
Keywords: Microfluidics
Resistance Artery
Automation
World to chip interconnect

In this thesis, I present a microfluidic platform that enables automated image-based assessment of biological structure and function. My work focuses on assessing intact resistance arteries from the mouse cerebral vascular bed with a diameter of approximately 120µm in vitro. The experimental platform consists of a microfluidic device and a world-to-chip fluidic interconnect that minimizes unwanted dead volumes and eliminates the need for any liquid-filled peripheral equipment. The integrated platform is computer controlled and capable of fully automated operation once a small blood vessel segment is loaded onto the chip. Robust operation of the platform was demonstrated through a series of case studies that assessed small artery function and changes therein induced by incubation with the drug nifedipine, a dihydropyridine calcium channel blocker. In addition artery segments were stained for L-type calcium channels, F-actin and nuclei, from which structural information about cell alignment and shape was quantified.
The Problem of Translation in Modern China: A Brief Study on Lu Xun and Qian Zhongshu
Keywords: Translation Theory
Early Modern China

This thesis aims to explore translation theory in modern China to shed light on the thought of inter-culturality through translation in the age of globalization, focussing on the works of Lu Xun and Qian Zhongshu. This paper attempts to unveil the constitution of modernization, the cultural way of crossing boundary, and the construction of imaginary otherness. The first chapter examines the methodological problems of translation in Lu Xun and Qian Zhongshu, separately, to demonstrate their contributions to Chinese modern translation theory from aesthetic viewpoints. The second chapter discusses the purpose of translation, investigating the cultural meaning of boundary crossing in translation. The third chapter examines the problems of the translatability and untranslatability from Lu Xun’s and Qian Zhongshu’s aspects, by contrasting with the concept of differences and translatability in post-structuralism theory, discussing the possibilities of mutual understanding between two cultures and languages through the imagined other in translation.
The Accuracy of Epidemiologic Definitions of Childhood Asthma Using a Clinical Reference Standard
Keywords: Asthma
Epidemiology
Diagnosis
Pediatric
Administrative database
Questionnaire

This study determined the sensitivity and specificity of questionnaires and administrative databases compared to a clinical reference standard for asthma. 208 schoolchildren from a population-based sample participated. They underwent a physician assessment, spirometry, methacholine challenge, exhaled nitric oxide and skin testing. Data was linked to the Ontario Asthma Surveillance Information System. “Questionnaire diagnosis” was an affirmative response to physician-diagnosed asthma. “Database diagnosis” was 2 outpatient visits or 1 hospitalization within 2 years. “Clinical diagnosis” required a physician assessment and objective findings of asthma. “Questionnaire diagnosis” of asthma was specific (92.1%) but not sensitive (75.3%) compared to the “clinical diagnosis”. “Database diagnosis” was sensitive (87.5%) but not specific (64.8%). Both sources had an excellent negative predictive value (97-98%) but poor positive predictive value (24-55%). Epidemiologic methods accurately identify those without asthma but are poor at identifying those with asthma, leading to an overestimation of asthma prevalence and dilution of risk estimates.
Identification of Cellular Components Interacting with the Shiga-like Toxin 1 A1 Chain (SLT-1 A1)
Keywords: protein toxin
shiga-like toxin 1

Shiga-like toxin 1 (SLT-1) is produced by Escherichia coli strains like the pathogenic strain O157:H7. These bacterial strains are responsible for worldwide cases of food poisoning and water contamination, and the toxin is a major cause of hemorrhagic colitis and the hemolytic uremia syndrome. SLT-1 is defined as a type II ribosome-inactivating protein (RIP) and belongs to a family of plant and bacterial AB toxins. The A1 chain blocks protein synthesis in eukaryotic cells by depurinating a single adenine base in 28S rRNA. The mechanisms by which the A chain of SLT-1 interacts with the host components to route itself to the cytosol remains largely unknown. This thesis project identified a list of putative cellular proteins that interact with the SLT-1 A1 chain by the use of yeast-2-hybrid (Y2H) screens and HeLa lysate pull down/mass spectrometry analyses. Further assessment of the top 8 host interactors did not yield true interactions.
The MET Receptor Tyrosine Kinase Is a Potential Therapeutic Target in Combination with Radiation in Head and Neck Squamous Cell Carcinoma
Keywords: Radiosensitizer
Receptor Tyrosine Kinase
Radiation
MET
Radioresistance
Clonogenic Survival

Radioresistance is a major cause of treatment failure and relapse in head and neck squamous cell carcinoma (HNSCC). Novel molecular targets need to be identified to increase cure rates and radiosensitivity in HNSCC. The MET receptor tyrosine kinase is highly dysregulated in cancer and plays a role in tumourigenesis, chemoresistance, and radioresistance. However, the role of MET in HNSCC radioresistance has not yet been investigated and may potentially be a radiosensitizing target. We discovered MET expression and intact ligand-induced signalling in HNSCC cell lines. Small molecule MET kinase inhibitors inhibited ligand-induced MET activation and downstream signalling. These inhibitors decreased HNSCC cell proliferation and clonogenic survival. Similarly, short-interfering RNAs targeting MET also decreased cell proliferation. The combination of radiation with the MET kinase inhibitors decreased clonogenic survival in an additive manner. Cell cycle analyses demonstrated that MET inhibitors alone or in combination with radiation induced small increases in sub-G1 cell populations.
The Role of TLR2 in the Pathogenesis of Kawasaki Disease
Keywords: TLR2
Kawasaki Disease
Endothelial cells

Kawasaki disease (KD) is a childhood vasculitis with a predilection for the coronary arteries (CA). The etiology of KD is unknown; however, superantigens (SAg) have been implicated. SAg-activated T cells undergo massive proliferation followed by apoptosis; conversely, in KD these T cells may persist and target the CAs. Enhanced costimulation can rescue SAg-activated T cells from apoptosis, and Toll-like receptor 2 (TLR2) enhances costimulation. In a murine model of KD, TLR2-deficient mice are disease resistant, and evidence suggests preferential expression of TLR2 at the CA. Results from this study demonstrate that TLR2 is rapidly expressed in the heart following disease induction, and that TLR2 is expressed differentially in various arteries. The aorta, from which the CAs branch off, expressed the highest TLR2 levels. A microvascular endothelial cell line was shown to function as an APC following TLR2 stimulation, supporting the proliferation of SAg-activated T cells and their rescue from apoptosis.
Aggression and Competition in Two Boreal Animals
Keywords: aggression
Cyphoderris
chipmunk

This thesis will focus on the use of agonistic behaviour in both direct and indirect competition in two arboreal species: the eastern chipmunk (Tamias striatus) and Cyphoderris monstrosa, a primitive orthopteran insect. Chipmunks are an example of indirect competitors as they are competing for finite resources for dealing with abiotic stresses to ensure their survival. Chapter 2 of this project investigates the behavioural time budget for above ground activity at a time of critical importance for overwinter survival. The chipmunks in this study displayed a focus of their time budget on eating and collecting food in preparation of winter survival while they were above ground. Chapter 3 of this research project studies the physiological differences between males of Cyphoderris monstrosa which engage in aggressive territorial contests. The data suggest that metabolic scope is correlated with RHP. Males’ ability to mobilize energy reserves may be an important factor in contest outcomes.