VictorJuiz/thesis huggingface dataset:
The Immunoregulatory Role of FGL2 as a Novel Effector Molecule of Treg Cells
Keywords: Immunology
Regulation
Treg cells
FGL2

Background and Aim: CD4+CD25+ regulatory T (Treg) cells are important in the maintenance of self-tolerance, regulation of T cell homeostasis, prevention of allograft rejection and in the modulation of immune responses to pathogens. Several groups have recently reported an increased expression of fgl2 in Treg cells by microarray analysis. FGL2, a member of the fibrinogen-like family, was previously shown to act as an immunomodulator by inhibiting DC maturation and T cell proliferation. Based on these findings, the immunoregulatory role of FGL2 as a novel effector molecule of Treg cells was investigated. Results: In agreement with previous studies, high levels of fgl2 transcripts were detected in Treg cells by real-time PCR. In fgl2-/- mice, an increased number and percentage of Treg cells were found with a greater expression of Foxp3 compared with fgl2+/+ Treg cells; however, the suppressive activity of fgl2-/- Treg cells was significantly impaired. Antibody to FGL2 completely inhibited the activity of fgl2+/+ Treg cells in vitro. Consistent with FGL2 contribution to Treg cell activity, targeted deletion of the gene led to an increased immune reactivity of DC, T cells and B cells, and the manifestation of glomerular autoimmunity in aged fgl2-/- mice. The importance of FGL2 as an effector of Treg cells was also demonstrated in MHV-3-induced fulminant hepatitis. Uninfected susceptible BALB/cJ mice had increased numbers of Treg cells and expression of FGL2 compared to uninfected resistant A/J mice. Following MHV-3 infection, plasma levels of FGL2 in BALB/cJ mice were significantly increased, correlating with an increased percentage of Treg cells. Treatment with anti-FGL2 antibody completely inhibited Treg cell activity and protected susceptible BALB/cJ mice against MHV-3-induced liver injury and mortality. Adoptive transfer of fgl2+/+ Treg cells into resistant fgl2-/- mice increased their mortality following MHV-3 infection. Finally, FGL2 treatment led to prolonged skin graft survival in a murine allotransplant model. The suppressive activity of FGL2 was mediated through binding to the inhibitory FcγRIIB receptor expressed on APCs, resulting in inhibition of DC maturation and induction of B cell apoptosis. Conclusion: These studies indicate that FGL2 plays an important immunoregulatory role as an effector cytokine of Treg cells; targeting FGL2 may provide a novel therapeutic approach for the treatment of patients with viral hepatitis, autoimmunity and transplant rejection.
Perceptions of Chinese International Students in Singapore: Adjustment Issues and Support
Keywords: Higher Education

The number of students choosing to engage in international education is increasing. Typically, the United States has been the number one destination for international students but that has declined in recent years (2002 to 2006). This implies that international students choose to study in other English speaking countries or in countries that may be more similar in culture. The purpose of this study was to explore the thoughts, opinions, and experiences of undergraduate Mainland Chinese international students who were studying at a university in Singapore. The focus was to understand their perceptions of the adjustments issues they faced as international students. Twenty-one (21) students were interviewed using a semi-structured face-to-face technique. A review of the literature was conducted. The three major areas of difficulty identified were learning to speak English, weather, and food. The most helpful support reported by the Chinese international students was the ‘English Bridging course’. Overall, general adaptation to life and study in Singapore presented no major obstacles for the Chinese international students and this resulted in low utilization of and need for existing support services. The respondents reported that learning to speak English was the most difficult adaptation they encountered due to the widespread use of Mandarin and Singlish by the local population. Interestingly the issues identified in other research studies such as difficulty with general living adjustment, personal psychological adjustment, social adjustment, and culture shock were not supported in this research. Also, loneliness, homesickness, anxiety, lack of social support, and difficulty with finances were not identified as major issues for this group of Chinese international students. This led the researcher to conclude that the more similar a host country is to the home country in culture and language, the easier the general adjustment of the international student. Also, the researcher concluded that the similarity in language (Mandarin) from the home country (China) to the host country (Singapore) is both a negative and a positive – it can greatly and positively facilitate general adjustment of an international student but it can hinder the English language adjustment and learning. Suggestions for future research were included in this study.
Testing the Maternal Response Hypothesis in Cases of Suspected or Substantiated Child Sexual Abuse: Secondary Data Analysis of the Canadian Incidence Study of Reported Child Abuse and Nelect, 1998
Keywords: Child sexual abuse
Maternal response

This dissertation’s analyses examined the association of caregiver, child, abuse and investigation characteristics with maternal response and emotional harm among families for whom child sexual abuse (CSA) was suspected or substantiated. Method This study was based on secondary analysis of data collected in the Canadian Incidence Study of Report Child Abuse and Neglect 1998. The current analysis was limited to 373 CSA investigations for which there was a female non-offending caregiver and complete data on maternal response. Bivariate and hierarchical logistic regression analysis was conducted for two outcomes; maternal response and emotional harm. Results According to social worker assessment, the majority of female non-offending caregivers (87.1%) of children investigated for suspected or substantiated child sexual abuse responded with belief of the abuse disclosure, emotional support and protection of the child victim. The overall maternal response model was significant and accounted for 40.8% of the variance (Nagelkerke R2). Factors significantly associated with maternal response in the multivariate model included maternal mental health, age of the child, child’s manifestation of sexualized behavior, child’s relationship to the perpetrator, duration of abuse and co-occurring maltreatment. The overall emotional harm model was significant and accounted for 18.3% of the variance (Nagelkerke R2). Age of the child at the time of investigation, inappropriate sexualized behavior and substantiation level were significant predictors in the final block of the emotional harm regression equation. Maternal response was no longer significantly associated with emotional harm when the analysis adjusted for child characteristics. Conclusion Consistent with previous research, the majority of non-offending mothers investigated as part of the CIS-98 responded to CSA disclosure with belief, emotional support and protection as determined by the social worker’s assessment. The current study supports the cumulative evidence that caregiver mental health, age of the child and the child’s relationship with the offender are significant predictors of negative maternal response and emotional harm. Although negative maternal response failed to predict emotional harm among children investigated for CSA, continued examination of the risk factors associated with maternal response is warranted to ensure the safety of a small, yet vulnerable segment of children.
Multi-factor Energy Price Models and Exotic Derivatives Pricing
Keywords: Mathematical finance
Energy derivatives
Option pricing and hedging
Singular perturbation

The high pace at which many of the world's energy markets have gradually been opened to competition have generated a significant amount of new financial activity. Both academicians and practitioners alike recently started to develop the tools of energy derivatives pricing/hedging as a quantitative topic of its own. The energy contract structures as well as their underlying asset properties set the energy risk management industry apart from its more standard equity and fixed income counterparts. This thesis naturaly contributes to these broad market developments in participating to the advances of the mathematical tools aiming at a better theory of energy contingent claim pricing/hedging. We propose many realistic two-factor and three-factor models for spot and forward price processes that generalize some well known and standard modeling assumptions. We develop the associated pricing methodologies and propose stable calibration algorithms that motivate the application of the relevant modeling schemes.
Developmental Measures of Morphosytactic Acquisition in Monolingual 3-, 4-, and 5-year-old Spanish-speaking Children
Keywords: Spanish
Language Acquisition

This research investigated aspects of the morphosyntactic language development of 115 3-, 4-, and 5-year-old monolingual Spanish-speaking preschool children who resided in Cali, Colombia. Two general language measures were collected from the children: a standardized receptive vocabulary measure (Test de Vocabulario en Imágenes, TVIP), and a parental report of speech and language problems. In addition, morphosyntactic measures of language development were obtained using both a story retelling and an elicitation task. Developmental language measures such as number of T-units (NU-TU), mean length of T-units (MLTU), subordination index (SUB-I), and grammatical errors per T-unit (GRE-TU) were derived from the narratives. Percentages of correct use of direct and indirect object pronouns, reflexive pronouns, definite articles, indefinite articles, plurals and adjectives were obtained from an elicitation task that was specifically designed for this study. Counts of use of these grammatical structures were also calculated from the narratives. There were no statistically significant differences between the three age groups on standard scores for the TVIP or scores for the parent questionnaires, indicating that the three age groups were comparable. For the developmental language measures there was an increasing developmental pattern for NU-TU, MLTU and SUB-I, but no changes were found for GRE-TU. Statistically significant changes for the productive use of the grammatical structures of interest to this study were almost always seen between 3 and 4 years of age. Adult use of these grammatical structures was always statistically significantly more correct than child productions. This investigation provides novel normative data for NU-TU, MLTU, SUB-I and GRE-TU for preschool children. This investigation also offers original data on the productive use of object pronouns, articles, adjectives and plurals across the preschool years. The language battery used in this investigation proved to be sensitive to developmental changes between 3 and 4-5 year olds and has the potential to be used as an eventual diagnostic tool for the identification of children with language disorders. Speech-language pathologists who work with Spanish-speaking children will be able to use this normative information to conduct more objective language assessments.
Karel Capek's Travels: Adventures of a New Vision
Keywords: Czech literature
Karel Capek
travel writing
travel and cultural identity
intermediality and narratology

This dissertation examines the theme of travel in the work of Karel Čapek (1890-1938), both in his travelogues and fiction. Instead of assuming travel as a conventional departure to another destination, journey and return home, Čapek experimented with the topic, popular in interwar literatures and arts, as an example of the avant-garde interconnectedness between different genres and arts. Čapek used three approaches to express his experiences of traveling. First, he founded his own aesthetics of the so called “marginal forms” or “low-brow genres” which he simultaneously interpolated in his prose. Their use, which greatly changes the perspective on travel writing, is visible in comparison between Čapek’s and previous travelogues (chapter 1). Secondly, he introduced skaz as stylized spoken language to Czech literature, and changed the traditional roles of the narrator and his addressees in travelogues (chapter 2). Thirdly, he used visual elements of language, combined verbal and visual arts (illustrations and drawings) in the narrative (chapter 3). Finally, all these elements he interpolated to his prose (chapter 4) through the intertextual links with travelogues. On the example of the theme of travel in Karel Čapek’s work, my dissertation revisits some current definitions of the historical avant-garde. It shows that the recent theories, predominantly developed on the examples from Western European and Russian arts, cannot be fully applied to local artistic movements. First, it shows that the notion of the avant-garde cannot be just confined to the writers who called themselves “avant-garde” (such as Karel Teige or Vladislav Vančura). Instead, it should be also expanded to other writers, such as Karel Čapek, marginal to the avant-garde mainstream. Second, the analysis of the theme of travel in Karel Čapek’s opus shows that the Czech avant-garde was not destructive towards its literary heritage. Instead, it offered an alternative reading of tradition through artistic experiments. In extension, it also provided a new understanding of the cultural and literary identity.
A White Wedding? The Racial Politics of Same-sex Marriage in Canada
Keywords: Lesbian and Gay
Legal
Critical Race

In A White Wedding? The Racial Politics of Same-Sex Marriage, I examine the inter-locking relations of power that constitute the lesbian/gay subject recognized by the Canadian nation-state as deserving of access to civil marriage. Through analysis of legal documents, Parliamentary and Senate debates, and interviews with lawyers, I argue that this lesbian/gay subject achieves intelligibility in the law by trading in on and shoring up the terms of racialized neo-liberal citizenship. I also argue that the victory of same-sex marriage is implicated in reproducing and securing a racialized Canadian national identity as well as a racialized civilizational logic, where “gay rights” are the newest manifestation of the modernity of the “West” in a post-9/11 historical context. By centring a critical race/queer conceptual framework, this research project follows the discursive practices of respectability, freedom and civility that circulate both widely and deeply in this legal struggle. I contend that in order to successfully shed its historical markers of degeneracy, the lesbian/gay subject must be constituted not as a sexed citizen but rather as a neoliberal citizen, one who is intimately tied to notions of privacy, property, autonomy and freedom of choice, and hence one who is racialized as white. The critical race/queer analytic also attends to the temporal and spatial registers framing this legal struggle that re-install various troubling racial hierarchies in a “gay rights” project often lauded as progressive. This analysis of the discursive terrain of same-sex marriage reveals the race shadow that lies at the heart of this equality-rights struggle. The conclusion of this thesis provides reflections for developing an ethics of activism that dislodges and resists the (re)production of racialized relations of power in lesbian and gay equality rights activism. In so doing, I seek to provoke, question and re-draw the landscape of our thinking, not only about same-sex marriage but also about the terms with which we conceive, articulate and practice racial and sexual justice.
Green Ethics and Green 'Faith': An Exploration of Environmental Ethics and Spirituality in a Technological Age
Keywords: religion
environment
ethics

The main concern of this dissertation is exploring and elucidating the nature and the relationship of religion/spirituality and ethics in the context of environmentalism, within the larger arena of liberal, technological society. It is driven foremost by a need for clear understanding of not only what these terms mean and what they represent, but also what it all means for where we stand today as ethical and spiritual beings. For in pursuing this topic, one must necessarily ask larger questions, namely: What does it mean to be ethical in technological society? What does it mean to be ‘spiritual’ in a ‘secular’ society? Are either of these things even possible? These questions form the backdrop of my particular focus on environmentalism. Through analysis of my own ethnographic research with members of the Sierra Club of Canada, and through use of the theoretical frameworks provided by four primary thinkers (Juan Luis Segundo, Alasdair MacIntyre, Charles Taylor and Jacques Ellul), I conclude that environmentalism, though far from problem-free, represents a ‘healthy’ form of ethical and spiritual expression in modern technological society. Part of this conclusion is the position that we are still very much ethical beings in technological society, and very much spiritual beings in secular society (though the latter is far more dependent on individual definitions of this term), and that in fact these two things relate directly.
Novel Self-categorization Overrides Racial Bias: A Multi-level Approach to Intergroup Perception and Evaluation
Keywords: social cognitive neuroscience
self-categorization
social identity
prejudice

People engage in a constant and reflexive process of categorizing others according to their race, gender, age or other salient social category. Decades of research have shown that social categorization often elicits stereotypes, prejudice, and discrimination. Social perception is complicated by the fact that people have multiple social identities and self-categorization with these identities can shift from one situation to another, coloring perceptions and evaluations of the self and others. This dissertation provides evidence that self-categorization with a novel group can override ostensible stable and pervasive racial biases in memory and evaluation and examines the neural substrates that mediate these processes. Experiment 1 shows that self-categorization with a novel mixed-race group elicited liking for ingroup members, regardless of race. This preference for ingroup members was mediated by the orbitofrontal cortex – a region of the brain linked to subjective valuation. Participants in novel groups also had greater fusiform and amygdala activity to novel ingroup members, suggesting that these regions are sensitive to the current self-categorization rather than features associated with race. Experiment 2 shows that preferences for ingroup members are evoked rapidly and spontaneously, regardless of race, indicating that ingroup bias can override automatic racial bias. Experiment 3 provides evidence that preferences for ingroup members are driven by ingroup bias rather than outgroup derogation. Experiment 4 shows that self-categorization increases memory for ingroup members eliminating the own-race memory bias. Experiment 5 provides direct evidence that fusiform activity to ingroup members is associated with superior memory for ingroup members. This study also shows greater amygdala activity to Black than White faces who are unaffiliated with either the ingroup or outgroup, suggesting that social categorization is flexible, shifting from group membership to race within a given social context. These five experiments illustrate that social perception and evaluation are sensitive to the current self-categorization – however minimal – and characterized by ingroup bias. This research also offers a relatively simple approach for erasing several pervasive racial biases. This multi-level approach extends several theories of intergroup perception and evaluation by making explicit links between self-categorization, neural processes, and social perception and evaluation.
Morphine-induced Locomotion and Dopamine Efflux in Mice: Role of M5 Muscarinic Receptors and Cholinergic Inputs to the Ventral Tegmental Area
Keywords: acetylcholine
mesolimbic dopamine
opiates
nicotinic
exploration
electrochemistry

M5 muscarinic receptors are associated with dopamine neurons of the ventral tegmental area (VTA) and substantia nigra, and provide an important excitatory input to the mesolimbic dopamine system. Here, I studied locomotion induced by systemic morphine (3, 10, 30 mg/kg, i.p.) in M5 knockout mice of the C57Bl/6 (B6) and CD1 x 129SvJ (129) background strains. M5 knockout mice of both strains showed reduced locomotion in response to 30 mg/kg morphine, while only B6 M5 knockout mice showed reduced locomotion in response to 10 mg/kg morphine. In B6 wild-type mice VTA pre-treatment with the non subtype-selective muscarinic receptor antagonist atropine (3 mg per side), but not the non subtype-selective nicotinic receptor antagonist mecamylamine (5 mg per side), reduced locomotion in response to 30 mg/kg (i.p.) morphine to a similar extent as systemic M5 knockout, suggesting that the reduced morphine-induced locomotion in M5 knockout mice was due to the loss of M5 receptors on VTA dopamine neurons. By contrast, in M5 knockout mice, either intra-VTA atropine or mecamylamine alone increased locomotion by almost 3 times relative to saline, and potentiated morphine-induced locomotion. Therefore, in M5 knockout mice, more clearly than in wild-type mice, blockade of either VTA muscarinic or nicotinic receptors activated locomotion. Infusions of morphine (50 ng) into the VTA increased nucleus accumbens dopamine efflux in urethane-anesthetized wild-type mice. Either M5 knockout or pre-treatment with VTA scopolamine (50 ug) in wild-type mice blocked accumbal dopamine efflux in response to VTA morphine. Therefore, M5 receptors are critical for excitation of dopamine neurons by intra-VTA morphine, suggesting that the reduced locomotion produced by systemic morphine in M5 knockout mice was, in part, due to loss of M5-mediated excitation of VTA dopamine neurons by opiates. The locomotion data also show that in the absence of M5 receptors, cholinergic afferents to mesolimbic dopamine neurons are inhibitory. This supports and extends the conclusions from many studies that non-M5 muscarinic receptors inhibit, and M5 receptors excite, dopamine neurons. Loss of M5-mediated excitation results in reduced acute effects of opiates.