VictorJuiz/thesis huggingface dataset:
Who makes international law? How the World Health Organization changed the regulation of infectious disease
Keywords: International Health Regulations
World Health Organization

This thesis investigates the impact of international organizations on the making of international law by applying insights on how international organizations work—or fail to work—to the process of institutionalized treaty making. Specifically, I probe the relationship between the World Health Organization (“WHO”) and international infectious disease law, focusing in particular on the 2005 International Health Regulations (“2005 IHR”), which was negotiated, adopted, and is now being implemented under WHO’s auspices.
The 2005 IHR is the most recent development in international infectious disease law, the history of which extends back to the beginning of international health cooperation in the mid-nineteenth century, before any international health organization was formed. Relying on secondary sources, WHO documents, archival materials, and personal interviews, I chronologically trace the evolution of international infectious disease law across changing institutional settings. I first examine the incremental growth of the older “barrier” approach to infectious disease regulation, initially developed in the absence of any international health organization and then with the aid of one of WHO’s predecessor organizations. I then analyze the decline of the barrier approach and the rise of the new “epidemiological” approach embodied by the 2005 IHR, with the aid of WHO.
Based on my empirical analysis, I conclude that WHO has radically changed the process of making international infectious disease law as well as its content. On its own initiative and without member state demand, WHO’s permanent staff experimented with novel practices that subsequently became the basis for the 2005 IHR. WHO’s work reduced the length of formal negotiation needed to arrive at a new agreement and the uncertainty associated with adopting a novel regulatory system. Its influence also raises normative questions about the proper role of international organizations in making international law—questions that require further exploration.
Taming the dragon: rural land takings law in modern China
Keywords: law
China
land expropriation
property

This thesis examines the theories and practices surrounding the rural land takings law in modern China. It identifies and rejects the dominant Transition Paradigm in the existing literature which treats the contemporary crisis of rural land expropriation in China as a case of unfulfilled constitutional promise and dispenses prescriptions aimed at developing the Chinese expropriation law towards the supposedly more advanced models of takings law found in other countries, especially the USA. By unearthing the long overlooked historical lineage within which the rural land takings law evolves over the past century in China, including the almost forgotten original takings clause in the 1954 PRC Constitution and the foundational theories propounded by both the communist and nationalist parties, this thesis offers a much richer picture on how and why the Chinese expropriation law has become the way it is today. It shows that the widely recognized phenomenon of “land finance” is a symptom rather than cause of the problem, which lies in the deeply entrenched tradition of rural land takings for the party-state’s social transformative programmes, rendering the Chinese experience incomparable to that of any other countries. The current takings clause in the 1982 PRC Constitution and the present law in this area, despite the relevant reforms over the past decade, remain to faithfully reflect such a tradition characterized by the state’s plan-based top-down control over rural land and the presumption of state expropriation in non-agricultural use of rural land. Since these have been the paramount features of the Chinese land regime for over half a century and are unlikely to change in the foreseeable future, most of the reform recommendations made in the existing scholarship are either irrelevant or unfeasible. However, this is not to say that no change is possible. A more modest yet more realistic reform proposal will be put forward.
Quantitative measurement of pH in stroke using chemical exchange saturation transfer magnetic resonance imaging
Keywords: Amide Proton Transfer (APT)
Magnetic Resonance Imaging (MRI)
Chemical Exchange Saturation Transfer (CEST)
pH
Stroke

Stroke is one of the leading causes of death and adult disability worldwide. The major therapeutic intervention for acute ischemic stroke is the administration of recombinant tissue plasminogen activator (rtPA) to help to restore blood flow to the brain. This has been shown to increase the survival rate and to reduce the disability of ischemic stroke patients. However, rtPA is associated with intracranial haemorrhage and thus its administration is currently limited to only about 5% of ischemic stroke patients. More advanced imaging techniques can be used to better stratify patients for rtPA treatment.
One new imaging technique, chemical exchange saturation transfer (CEST) magnetic resonance imaging, can potentially image intracellular pH and since tissue acidification happens prior to cerebral infarction, CEST has the potential to predict ischemic injury and hence to improve patient selection. Despite this potential, most studies have generated pH-weighted rather than quantitative pH maps; the most widely used metric to quantify the CEST effect is only able to generate qualitative contrast measurements and suffers from many confounds. The greatest clinical benefit of CEST imaging lies in its ability to non-invasively measure quantitative pH values which may be useful to identify salvageable tissue.
The quantitative techniques and work presented in this thesis thus provide the necessary analysis to determine whether a threshold for the quantified CEST effect or for pH exists to help to define tissue outcome following stroke; to investigate the potential of CEST for clinical stroke imaging; and subsequently to facilitate clinical translation of CEST for acute stroke management.
Social dynamics in natural populations of Pseudomonas aeruginosa
Keywords: social evolution
microbes
evolutionary biology

Microbes rely on collective behaviours, such as communication and cooperation to survive and form communities. The majority of these social behaviours are mediated by the secretion of public good molecules into a shared environment such that they can be utilized by neighbouring cells. Therefore, individuals that engage in costly cooperative behaviour are susceptible to exploitation by selfish cheats that gain the benefit of cooperation without investing their share of the public good cost. Understanding such bacterial social interactions and the underlying molecular mechanisms gives insight into their complex social life in natural environments and can be used to develop alternative treatments for pathogenic bacteria that rely on such social interactions for virulence and to infect hosts. In this thesis I examine social behaviours expressed by the opportunistic pathogen, Pseudomonas aeruginosa. I develop an understanding of bacterial social dynamics, particularly competitive dynamics between cooperator and cheat strains and strains that engage in bacteriocin-mediated chemical warfare. I investigate bacterial cheat-cooperator systems in several ways: 1) I begin with a review describing the evolution of and response to cheating across a range of organisms and discuss the confusion that arises in identifying cheats particularly in microbial studies and therefore propose a key to identify cheating behaviour. 2) I empirically test whether cheating behaviour is context dependent in bacterial populations and reveal that the ability to cheat varies with the abiotic and social environment, which are two fluctuating conditions in natural environments. 3) I take an experimental approach to investigate why cheat invasion is not commonly observed in natural bacterial populations by testing the effect of cooperative bacterial growth dynamics on cheating ability. I find that secretion of public goods varies with bacterial growth dynamics and physiological growth stages which may explain why cheat invasion is more commonly observed in lab cultures and not in established natural populations. 4) In the final chapter I experimentally use natural isolates to examine the role of bacteriocins in mediating competition in pathogenic populations and find that contrary to empirical and theoretical work, bacteriocins do not play a significant role in strain competitive success and dominance. The thesis has laid groundwork for studying and understanding the role of social behaviours in bacterial systems and for further exploring social dynamics in natural bacterial populations.
Two-dimensional colloidal systems: grain boundaries and confinement
Keywords: optical tweezers
soft matter
colloids
grain boundaries
optical microscopy
glasses
crystallisation

The behaviour of colloidal particles in two-dimensional (2D) systems is addressed in real space and time using magnetic fields, optical tweezers and optical video microscopy. First, the fluctuations of a grain boundary in a 2D colloidal crystal are analysed. A real space analogue of the capillary fluctuation method is derived and successfully employed to extract the key parameters that characterise the grain boundary. Good agreement is also found with a fluctuation-dissipation based method recently suggested in simulation. Following on from analysis of the interface fluctuations, the properties of the individual grain boundary particles are analysed to investigate the long standing hypothesis that suggests that grain boundary particle dynamics are similar to those in supercooled liquids. The grain boundary particle dynamics display cage breaking at long times, highly heterogeneous particle dynamics and the formation of cooperatively moving regions along the interface, all typical behaviour of a supercooled liquid. Next, the frustration induced by confining colloidal particles inside a pentagonal environment is investigated. The state of the system is adjusted via two separate control parameters: the inter-particle interaction potential and the number density. A gradual crystalline to confined liquid-like transition is observed as the repulsive inter-particle interaction potential is decreased. In contrast, re-entrant orientational ordering and dynamical effects result as the number density of the confined colloidal particles is increased. Finally, the dynamics of colloidal particles distributed amongst a random array of fixed obstacle particles is probed as a function of both the mobile particle and fixed obstacle particle number densities. Increasing the mobile and the obstacle particle number density drives the system towards a glass transition. The dynamics of the free particles are shown to behave in a similar way to the normal glass transition at low obstacle density and more analogous to a localisation glass transition at high obstacle density.
Optimised label-free biomarker assays with electrochemical impedance spectroscopy
Keywords: Diabetes
Parkinson's Disease
Electrochemical Impedance Spectroscopy (EIS)
Immunoassays

There is huge academic interest and clinical need associated with the development of biomarker immunoassays where general aims are the generation of highly specific, convenient and sensitive sensing formats. In this project, a powerful electrochemical technique, electrochemical impedance spectroscopy (EIS), is applied in the establishment of powerful biomarker detecting protocols. Firstly, ultrasensitive, label-free and reusable insulin sensors, based on an antibody-PEGylated thiol self-assembly monolayer (PEG thiol SAM) interface, were produced and characterised via Faradaic EIS, presenting a detection limit (LOD) of 1.2 pM, a linear range across four orders of magnitude, and high sensitivity in even 50 % serum. By applying similar surface chemistry, a label-free biosensor, specific for the detection of α-synuclein antibodies, was fabricated. The α-synuclein interfaces used enabled the reliable detecting of this biomarker in patient sample serum. The concentration levels in the control and a patient group were determined to be significantly different, and, significantly, this difference was consistently across two different cohorts. Strikingly, this could potentially underpin an entirely new means of early Parkinson’s disease (PD) diagnosis. Non-Faradaic EIS methods were additionally applied to label-free insulin assays at both PEG thiol SAM and zwitterionic polymer film interfaces. The latter presented not only an exceptionally non-fouling interface, but also one seemingly both highly biocompatible and facilitating enhanced receptor: target binding. Finally, impedance assays, though potent, generally, operate by sampling only one of a limited number of available experimental variables, typically, Rct for Faradaic EIS, or C or Z for non-Faradaic EIS. Work carried out herein also explores the generation and utility of a portfolio of mathematically derived immittance functions all obtained from the same raw data sets. A particular focus was the examination of whether these were capable of increasing assay sensitivity and efficiency above normal impedance treatments.
Transcriptional regulation by non-coding RNAs in Saccharomyces cerevisiae
Keywords: S. cerevisiae
non-coding RNAs
transcription

Genome-wide studies in Saccharomyces cerevisiae have revealed that the majority of the genome is transcribed on both strands, producing both coding and non-coding RNAs (ncRNAs). Initially, these ncRNAs were regarded as spurious transcripts but some have since been shown to have important roles as transcriptional regulators. Very little is understood about how ncRNAs are initiated, terminated and processed or how this influences their function. To address these questions, the expression, stability, and subcellular localization of the ncRNAs at the endogenous GAL locus was analysed. This revealed a complex interleaved transcript map, challenging the conventional view of a transcription unit (TU) flanked by 5’ sequences or promoters (P) that initiate transcription and 3’ regions, known as terminators (T), which control events such as transcript cleavage, polyadenylation, export and transcription termination. By creating conventional (PGAL-T) or unconventional (PGAL-P) hybrid TUs at the GAL locus, in which a promoter or terminator is positioned downstream of a galactose-inducible promoter, this work shows that both promoters and terminators are able to initiate antisense transcription to yield stable antisense transcripts. The data suggest that terminators contribute to efficient but variable expression from the promoter. An unconventional P-P TU, lacking a terminator, is transcribed on both strands but the sense transcript remains at low levels, through the repressive action of antisense transcription, and is retained in the nucleus. In contrast, the conventional P-T bi-directional TUs are plastic, with the Rrp6 component of the nuclear exosome and TATA-like sequences in the 3’ UTR determining whether the predominant transcript is antisense or sense. By relieving the repressive action of antisense transcription, this allows high levels of sense transcript to accumulate in the cytoplasm, contributing to gene expression, supporting a novel mode of gene regulation involving components of RNA quality control pathways acting through the 3’ region of genes.
Investigating and developing beginner learners' decoding proficiency in second language French: an evaluation of two programmes of instruction
Keywords: French
Phonics
Second language writing systems
Instructed foreign language learning
Foreign language phonics
Decoding
Phonological decoding

Second language (L2) decoding – the sub-lexical process of mapping the graphemes of an alphabetic writing system onto the phonemes they represent – is argued to underpin various aspects of L2 learning, particularly vocabulary acquisition. Recently, second language acquisition research has shown increased interest in decoding, consistently finding evidence for L1-to-L2 transfer effects on learners’ processing mechanisms and outcomes. Correspondingly, studies conducted in Modern Foreign Language (MFL) classrooms in English secondary schools – an under-researched context – have found that beginner learners of French tend to (a) pronounce L2 words according to English decoding conventions and (b) make poor progress in this aspect of L2 learning. Recent official guidance for MFL teachers has addressed this problem by advocating an explicit focus on decoding, but there is a lack of convincing evidence (both in the MFL context and more widely) that explicit L2 decoding instruction can be effective. The current study therefore trialled two programmes of French decoding instruction for beginner MFL learners, delivered in ten- to fifteen-minute segments over around thirty lessons. Three intact secondary school classes followed a phonics-based approach; three classes from another school followed a programme in which learners were encouraged to derive the pronunciations of French graphemes from ‘source words’ in a memorized poem; and six classes in two other schools received no explicit decoding instruction. Participants (N=186) completed pre- and post-tests of French decoding; a sub-sample (N=15) also completed task-based self-report interviews. The two intervention groups made significantly more progress than the comparison group in terms of the number of graphemes pronounced ‘acceptably’, although the magnitude of the difference between the groups was small. Compared to the comparison group, the two intervention groups also appeared to show different and more extensive patterns of change in their realizations of individual graphemes, even where their pronunciations were still not ‘acceptable’. Finally, self-report data generally revealed little change in participants’ strategic reasoning, either in the intervention or comparison group. Together, these findings suggest that explicit instruction can improve beginner learners’ proficiency in decoding L2 French, but that their progress may follow a longer and more complex trajectory than simply moving directly from ‘incorrect’ to ‘correct’ forms. Further research is required to assess the effects (if any) of a given improvement in decoding proficiency on other language-learning outcomes; and to design and evaluate alternative programmes of instruction.
Towards the total synthesis of calyciphylline A-type Daphniphyllum alkaloids
Keywords: organic chemistry
organocatalysis
alkaloids
total synthesis

This thesis details the studies towards the total synthesis of the calyciphylline A-type Daphniphyllum alkaloids, with a particular focus on daphniyunnine D (23).
Chapter 1 introduces these biologically and synthetically interesting polycyclic natural products and describes our designed approach towards their synthesis. Separate studies targeting the construction of two tricyclic ring systems have been developed. These provide rapid entry to synthetically versatile intermediates, allowing for the potential synthesis of numerous members of the alkaloid family.
Chapter 2 describes the first study which focuses on the construction of the main tricyclic [6‒5‒7] ACD core 172 via a proton transfer/IMDAF reaction cascade as the main step. Large scale synthesis of the precursor to this cascade 164 has allowed for the successful investigation of an asymmetric variant giving rise to an enantioenriched adduct 104.
Chapter 3 describes a novel design for the construction of the [7‒5‒5] DEF tricycle common to 100+ Daphniphyllum alkaloids. An IMPKR, double-bond migration, allylic oxygenation protocol was first validated on a model system and later applied in combination with the synthetic route developed in chapter 2 to achieve the construction of the [6‒5‒7‒5‒5] ACDEF pentacycle 249.
Chapter 4 focuses on the construction of the piperidine ring B via an intramolecular gold-catalysed 6-exo-trig hydroalkylation. During the development of the route to daphniyunnine D, various intermediates were afforded which were further elaborated to provide appropriate cyclisation substrates for this study. Their synthesis combined with proof of principle experiments for the desired cyclisation conclude this dissertation work.
The construction of colonial identity in the Canadas, 1815-1867
Keywords: nationalism
history
Canada

This thesis examines the construction and contestation of Anglo-Canadian identity from the end of the War of 1812 until Confederation in 1867. It argues that the conflict between English- and French-speakers in the Canadas was by no means inevitable but a function of the institutional and political circumstances of the time. It seeks to complicate the picture of the British in Canada by demonstrating that they were a diverse community of different groups, institutions and religions that only through struggle and the incentives of party politics were able to unify themselves into a single culture. The development of party politics not just coincided with the creation of Anglo-Canadian identity but played a fundamental role in creating it. Through the burgeoning newspaper industry, the Reform and Tory parties spread their ideas of what it meant to be British, loyal and Canadian to a widespread English-speaking audience. Canadian history in this period is better understood not in the traditional dualist framework of British against French but as the complex interactions of many different groups, including the English, the Scots, the Irish Protestants, the Irish Catholics, the Americans and the French-Canadians. The thesis seeks to deconstruct the terms ‘British’ and ‘loyal’. Both terms were appropriated by various individuals and groups seeking to gain benefits by defining themselves as such. Until the early 1830s, attempts were made to include certain classes of French-Canadians within the broader British polity and identity. The 1837 rebellions marked the ‘othering’ of French-Canadians. Meanwhile the Upper Canada rebellions presented an enemy in the United States and a new strain of anti-Americanism, separate to that of the loyalists, was developed. By 1849, the moment of the rebellion losses crisis, the fundamental tenets of the Anglo-Canadian identity had been established: anti-Americanism, a concern about French political influence and a sense of kinship with English speakers across the province of United Canada. These three periods are shown to have played a crucial role in the development of an anglophone identity that encompassed the whole of United Canada.