VictorJuiz/thesis huggingface dataset:
The C. elegans p53 Family Gene cep-1 and the Nondisjunction Gene him-5 are Required for Meiotic Recombination
Keywords: meiotic recombination
p53
C. elegans

p53 promotes maintenance of genetic information either by causing apoptosis of damaged cells, or by altering the cell cycle and repair pathways such that damage can be accurately repaired. The nematode Caenorhabditis elegans possesses only one p53 family member, CEP-1, that controls apoptosis and the cell cycle in response to genotoxic stress. Mutation in the meiotic gene him-5 increases nondisjunction of the X chromosome, resulting in increased frequencies of XO male and XXX Dpy progeny, and it affects the frequency of meiotic recombination on X. him-5 is allelic to the ORF D1086.4, which encodes a putative basic protein with no clear homologues or domain structure. The modest embryonic lethality (Emb) of him-5 mutants is dramatically increased by mutation of cep-1 but no change is seen in the proportion of XO male or XXX Dpy progeny. The synergistic effects of cep-1 and him-5 mutation are independent of CEP-1's DNA damage regulators and other meiotic mutants, and they do not involve deregulated apoptosis. cep-1; him-5 double mutants have abnormal chromatin morphology in diakinesis-arrested oocytes reminiscent of that seen in double strand break (DSB) repair mutants. This phenotype depends on the presence of SPO-11-induced meiotic DSBs, suggesting CEP-1 and HIM-5 function together to promote accurate recombination during meiosis. In support of this hypothesis, cep-1; him-5 show a significant reduction in crossover frequency between autosomal markers compared to wild-type or either single mutant alone, suggesting they function together to promote meiotic crossing over. The X chromosome nondisjunction in both him-5 and cep-1; him-5 is a result of failure of DSB formation and subsequent chiasma formation on the X. However, the embryonic lethality phenotype of him-5 and cep-1; him-5 is caused by a defect either downstream or in parallel to meiotic DSB formation. The diakinesis chromatin phenotype of cep-1; him-5 suggests this defect may be in meiotic DSB repair. This is confirmed by the fact that cep-1; him-5 animals show more persistent meiotic DSB-associated RAD-51 foci staining compared to wild-type, suggesting CEP-1 and HIM-5 may function in efficient resolution of SPO-11-induced DSBs during meiosis. A role for CEP-1 in promoting accurate repair of DSBs during meiosis may be related to p53's function in promoting faithful meiotic recombination in mammalian cells. HIM-5's role in DSB formation and repair suggests another mechanistic link between these recombination steps. Meiotic recombination is vital for genome stability, and characterization of the role of CEP-1 and HIM-5 will increase our understanding of the p53 family and genetic redundancy at multiple steps in this process.
È caso da intermedio! Comic Theory, Comic Style and the Early Intermezzo
Keywords: music
intermezzo
Molière
comedy
commedia dell'arte
opera
Antonio Salvi
Leonardo Vinci
Giuseppe Maria Orlandini
Bernardo Saddumene
Descartes
Naples
Venice
humour
Medici
elastic gag

This dissertation is a study of the comic intermezzo’s literary origins and musical practice in the years before Pergolesi’s La serva padrona (1733). It begins with a chronological examination of Italian comic plays and operas written between 1660 and 1723. During these years comic playwrights adopted a style of writing speech from the improvised theatre which makes use of what Richard Andrews (1993) refers to as “elastic gags.” This style of comedy flourished under Medici patronage in Florence in the last decades of the seventeenth century and then spread to Venice, Rome and Naples during the first years of the intermezzo’s development. It is a style of comedy shared with the plays of Molière, and other contemporaneous French authors. This dissertation examines several scenes based on French works which have previously not been identified as having earlier sources. The decision to adapt these earlier sources for the intermezzo did not occur in a vacuum. The practice of comedy in the intermezzo was conditioned by the artistic, social and political climate of Italy. This study investigates the relationship between intermezzos and the milieus which produced them. The success of some intermezzos, like Il marito giocatore (1719), resulted from a combination of their artistic merit and their broad social appeal, while others, like Albino e Plautilla (1723), were musically adept but remained obscure because their humour was specific to the world they satirized. Both intermezzos are indebted to earlier French sources. Many others which are metatheatrical in nature draw on contemporary debates about opera. A final section examines selected arias from the intermezzo repertory using incongruity theory. Comic theory makes clear that the intermezzo’s musical language was not a new development. Just as librettists drew on earlier written traditions to form the literary text of the intermezzo, composers drew on existing musical practices to create humour. The intermezzo was therefore not naively comic—a portrait of the genre which is all too common—but rather a repertory which was thoroughly enmeshed within contemporary artistic practice and a wider social and cultural world.
System Availability Maximization and Residual Life Prediction under Partial Observations
Keywords: Availability maximization
Residual life prediction
Hidden Markov model
EM algorithm
Partially observable system
Time series modelling
Stochastic dynamic programming
Control limit structure
Multivariate Bayesian control chart
Condition-based maintenance
Semi-Markov decision process
Hidden semi-Markov model

Many real-world systems experience deterioration with usage and age, which often leads to low product quality, high production cost, and low system availability. Most previous maintenance and reliability models in the literature do not incorporate condition monitoring information for decision making, which often results in poor failure prediction for partially observable deteriorating systems. For that reason, the development of fault prediction and control scheme using condition-based maintenance techniques has received considerable attention in recent years. This research presents a new framework for predicting failures of a partially observable deteriorating system using Bayesian control techniques. A time series model is fitted to a vector observation process representing partial information about the system state. Residuals are then calculated using the fitted model, which are indicative of system deterioration. The deterioration process is modeled as a 3-state continuous-time homogeneous Markov process. States 0 and 1 are not observable, representing healthy (good) and unhealthy (warning) system operational conditions, respectively. Only the failure state 2 is assumed to be observable. Preventive maintenance can be carried out at any sampling epoch, and corrective maintenance is carried out upon system failure. The form of the optimal control policy that maximizes the long-run expected average availability per unit time has been investigated. It has been proved that a control limit policy is optimal for decision making. The model parameters have been estimated using the Expectation Maximization (EM) algorithm. The optimal Bayesian fault prediction and control scheme, considering long-run average availability maximization along with a practical statistical constraint, has been proposed and compared with the age-based replacement policy. The optimal control limit and sampling interval are calculated in the semi-Markov decision process (SMDP) framework. Another Bayesian fault prediction and control scheme has been developed based on the average run length (ARL) criterion. Comparisons with traditional control charts are provided. Formulae for the mean residual life and the distribution function of system residual life have been derived in explicit forms as functions of a posterior probability statistic. The advantage of the Bayesian model over the well-known 2-parameter Weibull model in system residual life prediction is shown. The methodologies are illustrated using simulated data, real data obtained from the spectrometric analysis of oil samples collected from transmission units of heavy hauler trucks in the mining industry, and vibration data from a planetary gearbox machinery application.
Modelling Detailed-chemistry Effects on Turbulent Diffusion Flames using a Parallel Solution-adaptive Scheme
Keywords: Computational Fluid Dynamics
Computational Combustion

Capturing the effects of detailed-chemistry on turbulent combustion processes is a central challenge faced by the numerical combustion community. However, the inherent complexity and non-linear nature of both turbulence and chemistry require that combustion models rely heavily on engineering approximations to remain computationally tractable. This thesis proposes a computationally efficient algorithm for modelling detailed-chemistry effects in turbulent diffusion flames and numerically predicting the associated flame properties. The cornerstone of this combustion modelling tool is the use of parallel Adaptive Mesh Refinement (AMR) scheme with the recently proposed Flame Prolongation of Intrinsic low-dimensional manifold (FPI) tabulated-chemistry approach for modelling complex chemistry. The effect of turbulence on the mean chemistry is incorporated using a Presumed Conditional Moment (PCM) approach based on a beta-probability density function (PDF). The two-equation k-w turbulence model is used for modelling the effects of the unresolved turbulence on the mean flow field. The finite-rate of methane-air combustion is represented here by using the GRI-Mech 3.0 scheme. This detailed mechanism is used to build the FPI tables. A state of the art numerical scheme based on a parallel block-based solution-adaptive algorithm has been developed to solve the Favre-averaged Navier-Stokes (FANS) and other governing partial-differential equations using a second-order accurate, fully-coupled finite-volume formulation on body-fitted, multi-block, quadrilateral/hexahedral mesh for two-dimensional and three-dimensional flow geometries, respectively. A standard fourth-order Runge-Kutta time-marching scheme is used for time-accurate temporal discretizations. Numerical predictions of three different diffusion flames configurations are considered in the present work: a laminar counter-flow flame; a laminar co-flow diffusion flame; and a Sydney bluff-body turbulent reacting flow. Comparisons are made between the predicted results of the present FPI scheme and Steady Laminar Flamelet Model (SLFM) approach for diffusion flames. The effects of grid resolution on the predicted overall flame solutions are also assessed. Other non-reacting flows have also been considered to further validate other aspects of the numerical scheme. The present schemes predict results which are in good agreement with published experimental results and reduces the computational cost involved in modelling turbulent diffusion flames significantly, both in terms of storage and processing time.
Disabled Young People, Support and the Dialogical Work of Accomplishing Citizenship
Keywords: Disability
Citizenship

Governments, human rights bodies and disability studies scholars all have suggested that disabled people’s citizenship – the legal status and lived practices that enable membership, participation and belonging in one’s community - depends on consistent, adequate and readily available home and personal supports. Yet, little theoretical or empirical work examines disabled young people’s citizenship or their use of support, particularly from their standpoints. Consequently, the ‘work’ disabled young people do to accomplish citizenship remains unrecognized, as are their unique requirements for support to do that work. Normative non-disabled citizenship assumptions remain unproblematized. This study explores what disabled young people do to accomplish citizenship, using home and personal support as the empirical foci. I used a dialogic theoretical and methodological approach, drawing on Mikhail Bakhtin and Dorothy Smith. Both posit that our talk, consciousness and actions respond to and anticipate the voices of others. Through participatory media arts techniques with disabled young people, ethnographic observation and interviews with gatekeepers to formal and informal care, I describe the work that a group of disabled young people did to secure and maintain support and how this in turn shaped their opportunities for specific citizenship practices: self-determination, community participation and social contribution. I argue that disabled young people's work to secure and maintain support requires that they mobilize the authoritative discourse of 'the poster child': a set of objectified values and views encapsulated in utterances about disabled people as futureless, deficient and deferential, originating in images to promote charitable giving. I trace three sequences of activities in which participants assimilated, resisted or brought poster child utterances into ‘dialogue’. The findings raise questions about the extent to which formal entitlements to supports influence how citizenship is lived. Drawing attention to the gaps and tensions in support provision, the findings illuminate the tremendous invisible, tacit work these participants do to strengthen fragile supports. This work, organized by philanthropic rather than rights discourses, leads to a qualified or fragile citizenship. Finally, the study raises questions about the normative and material demands that we may all experience with respect to achieving citizenship, regardless of disability or age.
Theory and Applications of Microstrip/Negative-refractive-index Transmission Line (MS/NRI-TL) Coupled-line Couplers
Keywords: RF/Microwave Coupler
Complex modes
Metamaterials
Coupled-mode theory
MS/NRI-TL coupler
Multi-conductor transmission lines
Right-half-plane branch-points
Coupling between forward and backward waves
Network theory of coupling
Independent excitation of complex modes
Backward waves
Negative group delay
High directivity coupler

The electromagnetic coupling of a microstrip transmission line (MS-TL) to a metamaterial backward wave Negative-Refractive-Index transmission line (NRI-TL) is the primary investigation of this dissertation. The coupling of forward waves in the MS-TL to the backward waves in the NRI-TL results in the formation of complex modes, characterized by simultaneous phase progression and attenuation along the lossless lines. Through network-theoretic considerations, we investigate the properties of these modes in the complex-frequency plane of the Laplace domain to help unravel the confusion that has existed in the literature regarding the independent excitation of a pair of conjugate complex modes. We show that it is possible to arbitrarily suppress one of the modes over a finite bandwidth and completely eliminate it at a discrete set of frequencies using proper source and load impedances. Hence we use conjugate modes with independent amplitudes in our eigenmode expansion when we analyse various coupling configurations between the two types of lines (MS/NRI-TL coupler). We derive approximate closed-form expression for the scattering parameters of the MS/NRI-TL coupler and these are complemented by design charts that allow the synthesis of a wide range of specifications. Moreover, these expressions reveal that such couplers allow for arbitrary backward coupling levels along with very high-isolation when they are made half a guided wavelength long. The MS/NRI-TL coupler offers some interesting applications which we highlight through the design and testing of a 3-dB power splitter, a high-directivity signal monitor and a compact corporate power divider. We have included design, simulation and experimental data for the fabricated prototypes exhibiting good agreement and thereby justifying the theory that has been developed in this work to explain the coupling between a right-handed MS-TL and a left-handed NRI-TL.
Patterns, Determinants, and Spatial Analysis of Health Service Utilization following the 2004 Tsunami in Thailand
Keywords: Natural disaster
Tsunami
Distance to a health facility
Distance decay effect
Grossman model
Health service utilization
Two-part model
Propensity score method

On December 26th, 2004, 280,000 people lost their lives. A massive earthquake struck Indonesia, triggering a tsunami that affected several countries, including Thailand. The disaster had important implications for health status of Thai citizens, as well as health system planning, and thus underscores the need to study its long-term effect. This dissertation examined the patterns, determinants, and spatial analysis of health service utilization following the tsunami in Thailand. The primary aim was to determine whether tsunami-affected status (personal injury or property loss) and distance to a health facility (public health center or hospital) influenced health service utilization. The study population included Thai citizens (aged 14+), living in the tsunami-affected Thai provinces: Phuket, Phang Nga, Krabi, and Ranong. Study participants were randomly selected from the ‘affected’ and ‘unaffected’ populations. One and two years after the tsunami, participants were interviewed in-person on demographic and socio-economic factors, disaster impact, health status, and health service utilization. Five types of health services were examined: outpatient services, inpatient services, home visits, medications, and informal (unpaid) care. Distance to a health facility was calculated using Geographic Information System’s Network Analyst. The Grossman model of the demand for health care and a distance decay concept provided the foundation for this study. A propensity score method and a two-part model were used to examine the study objectives. There were 1,889 participants. One year after the tsunami, individuals affected by property loss were more likely to use medications than unaffected participants. Two years after the tsunami, individuals with personal injury were more likely to use outpatient services, medications, and informal care than unaffected participants. Distance to a health facility was associated with the use of medications and informal care. The results confirmed the long-term effect of a tsunami. This dissertation may assist the decision- and policy-makers in the identification of those most likely to use health services and in the request of health resources to the affected areas. The patterns, determinants, and spatial analysis of health service utilization found in this study may not be specific to a tsunami and may provide insights on post-disaster contexts of other natural disasters.
Effect of Chemical Impurities on the Solid State Physics of Polyethylene
Keywords: Insulating Polymers
High Field Conduction
Chemical Impurities
Dielectrics
Polyethylene
Computational Quantum Mechanics
Density Functional Theory

Computational quantum mechanics in the frame work of density functional theory (DFT) was used to investigate the effect of chemical impurities on high field conduction in polyethylene (PE). The impurity states in the band gap caused by common chemical impurities were characterized in terms of their “depth”, i.e. energy relative to their relevant band edge (valence band or conduction band), and in terms of the extent to which their wavefunctions were localized to a single polymer chain or extended across chains. It was found that impurity states can affect high field phenomena by providing “traps” for carriers, the depths of which were computed from first principle in agreement with estimates in literature. Since the square of the wavefunction is proportional to the spatial electron probability density, transfer of charge between chains requires wavefunctions which are extended across chains. Impurity states which are extended between chains can facilitate the inherently limited interchain charge transfer in PE, as the DFT study of iodine doped PE revealed. The introduction of iodine into PE increases conductivity by several orders of magnitude, increases hole mobility to a much greater extent than electron mobility, and decreases the activation energy of conduction from about 1 eV to about 0.8 eV. These characteristics were explained in terms of the impurity states introduced by iodine and wavefunctions of those states. Understanding the effect of iodine on conduction in PE provided a basis for understanding the effect of common chemical impurities on conduction therein. In particular, carbonyl and vinyl impurities create states which should promote hole mobility in a manner very similar to that caused by iodine. It was demonstrated that in the context of high field conduction in PE, besides the traditional focus on the depth of impurity states, it is important to study the spatial features of the states wavefunctions which are neither discussed nor accounted for in present models.
Concussions in the National Hockey League (NHL): The Video Analysis Project
Keywords: Concussion
National Hockey League
Injury Prevention
Video Analysis
Hockey
Sport

Hockey is a popular sport, and at its highest levels, it is a complex contact game characterized by physical strength, speed, and skill. The interaction of these characteristics contributes to the inherent risk of injury athletes must face while playing. Among hockey injuries, concussions are one of the most commonly sustained by athletes across all levels of play and age groups. Significant public attention, combined with poorly understood long-term effects, indicates the importance of tangible preventive strategies. The main goal of this thesis was to understand, through video analysis, how playing characteristics and mechanism of injury contribute to concussions in the National Hockey League (NHL). In the first study, the development and validation of an observational recording tool used to code and analyze NHL concussions observed via video analysis was described. The second study attempted to synthesize the description of players’ characteristics, antecedent events, and contextual variables associated with events leading to concussion at the NHL level. Several specific risk factors for concussion in NHL players were identified, including position, body size, specific locations on the ice, and particular situations based on a player's position. The final study systematically analyzed how concussions occur to identify potential pattern(s) of concussions. A common specific injury mechanism characterized by player-to-player contact and resulting in contact to the head by the shoulder, elbow, or gloves, was also identified. When the principal mechanism was refined further, several important characteristics were discernable: (i) contact was often to the lateral aspect of the head; (ii) the player who suffered a concussion was often not in possession of the puck; and (iii) no penalty was called on the play. Collectively, these studies served to address gaps in the literature; the implications for informing prevention and management strategies are also discussed.