VictorJuiz/thesis huggingface dataset:
Private tomb reliefs of the late period from Lower Egypt
Keywords: Ancient Egypt

This study considers the relief decoration of private tombs in Lower Egypt in the period 664-332 BC. The basis for analysis is a chronologically arranged descriptive catalogue, which includes both isolated blocks in museum collections and tombs whose location is known. The present condition of the relief and its content are described in detail there. Texts are considered where they provide infotmation on provenance and dating, and hand-copies are provided. Each piece is illustrated in the plate volume.
Enough of the material can be dated by textual evidence to provide a solid framework for stylistic ordering of the remainder. The resulting chronology has important implications, dividing the period into two major phases, covering the seventh and sixth centuries, and the fourth century, separated by a hiatus in production of tomb reliefs. The chronology proposed eliminates the possibility that either Greeks or Persians exercised any significant influence on Egyptian art before the very end of the period. Instead, native tradition emerges as the primary inspiration for Late Period artists. Two sources stand out. The first is the Old-Middle Kingdom tomb repertory (archaism), the second is the New Kingdom tradition carried on in the minor arts, a source largely-ignored hitherto. These were not slavishly copied, but adapted and "modernized" to suit the taste of the time. The independence and creativity of Late Period artists is emphasized. A discussion of stylistic development in light of the dating system is given, and several themes are analyzed in detail as illustrations of the larger issues raised.
Use of inertial sensors to measure upper limb motion: application in stroke rehabilitation
Keywords: Kalman filters
motion tracking
stroke patients
accelerometers
motion capture systems
gyroscopes
movement analysis
upper limb
inertial sensors
signal processing
metrics
rehabilitation

Stroke is the largest cause of severe adult complex disability, caused when the blood supply to the brain is interrupted, either by a clot or a burst blood vessel. It is characterised by deficiencies in movement and balance, changes in sensation, impaired motor control and muscle tone, and bone deformity. Clinically applied stroke management relies heavily on the observational opinion of healthcare workers. Despite the proven validity of a few clinical outcome measures, they remain subjective and inconsistent, and suffer from a lack of standardisation. Motion capture of the upper limb has also been used in specialised laboratories to obtain accurate and objective information, and monitor progress in rehabilitation. However, it is unsuitable in environments that are accessible to stroke patients (for example at patients’ homes or stroke clubs), due to the high cost, special set-up and calibration requirements.
The aim of this research project was to validate and assess the sensitivity of a relatively low cost, wearable, compact and easy-to-use monitoring system, which uses inertial sensors in order to obtain detailed analysis of the forearm during simple functional exercises, typically used in rehabilitation. Forearm linear and rotational motion were characterised for certain movements on four healthy subjects and a stroke patient using a motion capture system. This provided accuracy and sensitivity specifications for the wearable monitoring system. With basic signal pre-processing, the wearable system was found to report reliably on acceleration, angular velocity and orientation, with varying degrees of confidence. Integration drift errors in the estimation of linear velocity were unresolved. These errors were not straightforward to eliminate due to the varying position of the sensor accelerometer relative to gravity over time. The cyclic nature of rehabilitation exercises was exploited to improve the reliability of velocity estimation with model-based Kalman filtering, and least squares optimisation techniques. Both signal processing methods resulted in an encouraging reduction of the integration drift in velocity. Improved sensor information could provide a visual display of the movement, or determine kinematic quantities relevant to the exercise performance. Hence, the system could potentially be used to objectively inform patients and physiotherapists about progress, increasing patient motivation and improving consistency in assessment and reporting of outcomes.
The making of the Tuoba Northern Wei: constructing material cultural expressions in the Northern Wei Pingcheng Period (398-494 CE)
Keywords: Northern and Southern dynasties
capital cities
Tuoba
Xianbei
Northern Wei
Nomadic dynasties
Yungang
Buddhism
Northern China
Pingcheng

The Tuoba’s success in the making of the Northern Wei as a conquest dynasty in fifth century northern China will be argued in this thesis as a result of their ability to cross between the traditions and practices of the Chinese sphere and those of the Eurasian steppe, through the construction of a "dual presence" in the Pingcheng period (398-494 CE). A negotiation of material culture in this formative phase of state-building allowed for new notions of kingship, dynastic identity, and representations of daily life to be (re)created. This was manifested separately through the application of mountain-side stone sculptures, tomb repertoires, as well as the conception of Pingcheng as a capital city. The material cultural expressions explored in this thesis reflect significant changes in the socio-cultural atmosphere at this point in history. In effect, these ritual, funerary, and commemorative discourses wove together to create new notions of "Chineseness" in fifth century northern China. In the following discussion, we will come to recognize the Tuoba’s maintenance of a "dual presence", not only as "Son of Heaven" to the conquered subjects, but also carrying over practices that befit a Khagan in the Central Asian tradition, as an act of ingenuity.
Excitations in holographic quantum liquids
Keywords: quantum field theory at finite temperature and density
holography
gauge/gravity duality
strongly-coupled gauge theories
String theory
AdS/CFT correspondence

In this thesis we review the gauge/gravity duality and how it can be used to compute the thermodynamic properties and low-energy excitations of holographic quantum liquids - strongly-interacting field theories with a non-zero density of matter. We then study in detail the charge density excitations of two such liquids, the D3/D7 theory and the RN-AdS₄ theory, by computing the poles of their charge density Green's functions, and their charge density spectral functions. Although it is not a Landau Fermi liquid, the charge density excitations of the D3/D7 theory display many of the same properties as one, including a collisionless/hydrodynamic crossover as the temperature is increased. In contrast to this, the charge density (and energy density) excitations of the RN-AdS₄ theory do not share these properties but behave in a way that cannot be explained by Landau's theory of interacting fermionic quasiparticles. This is consistent with other results which indicate that this is not a Landau Fermi liquid.
Towards the development of vascularized constructs for bone repair
Keywords: endothelial cells
syngeneic
biomaterials
regenerative medicine
vascularization
FVB mice
bone marrow stromal cells
orthopaedic transplants

The development of a vasculature within a tissue-engineered construct is one of the largest hurdles to successful bone regeneration. This thesis investigates methods to increase vasculature of such transplanted constructs, based on in vivo transplant studies and in vitro analysis of cell behaviors. A syngeneic mouse model in immunocompetent mice was developed and analyzed for both osteogenesis and hematopoiesis. This study demonstrates that syngeneic bone marrow stromal cells (BMSCs) are not rejected by the host, provided the strain of mice is sufficiently inbred. Additionally, an effective protocol was developed for the isolation of endothelial cells (ECs) from the bone marrow of mice. Two different sets of materials for this study were analyzed, both collagen based, and the GelfoamTM scaffold was found to possess advantages over synthesized collagen or collagen/hydroxyapatite composites, although only for mouse and not human bone transplantation.
In order to gain rapid and integrated vasculature formation within the transplant, attempts were made to increase both (de novo) vasculogenesis and angiogenesis (ingrowth) from the surrounding tissue. For the former, transplant studies were combined with in vitro osteogenic calcification studies. Direct co-culture of the BMSCs and ECs increased osteogenic calcification and was monitored by using both alizarin red S quantification and quantitative polymerase chain reaction. Angiogenesis (as assessed by cell migration) was studied by various motility and chemotaxis assays in vitro, as well as through use of a directed in vivo angiogenesis assay. Growth factors, particularly TGF-β1 and BMP-4, were found to increase cell movement in these systems. In conclusion, we show that although much work remains to be done in order to increase the vasculature in bone transplants, systematic combination of in vivo and in vitro assays can elucidate the nature behind this crucial process in this context.
Direct taxation and the internal market: assessing possibilities for a more balanced integration
Keywords: non-binding coordination
tax (taxation)
EU law
European (EU) integration
the European Commission
direct taxation
the Internal Market
The European Union (EU)
tax coordination
exit tax
enforcement
the tax treatment of losses
tax integration
soft law
good governance
infringement procedure

The role of the Court of Justice of the European Union in the process of direct tax integration in the European Union (EU) has been widely discussed in the academic literature, while another important actor, the European Commission, has attracted much less attention. The Commission’s input is commonly perceived to be limited to the right of legislative initiative. This study questions such an oversimplified interpretation and draws a more nuanced picture of EU tax harmonisation by integrating an analysis of the complex regulatory approaches adopted by the Commission for the establishment and smooth functioning of the Internal Market.
It is argued that the past decade brought a notable change in the Court-dominated pattern of direct tax integration. This change is apparent first in the evolution of enforcement strategies under Articles 258 and 260 TFEU; and second, in the increased reliance upon non-binding regulatory measures. Both developments have strengthened the role of the Commission, supplying it with more possibilities for influencing national direct tax systems, despite the reluctance of Member States to agree upon legislative harmonisation. Against this background, the procedural and substantive problems associated with these instruments require closer examination.
The normative scope of this research covers the key procedural problems related to the infringement procedure and non-binding regulatory measures. The empirical component explores the Commission’s enforcement and coordination practices, drawing particular attention to two case studies: the tax treatment of losses in cross-border situations and exit taxation. Following the analysis of enforcement and coordination in the field of direct taxation, the study argues the need and proposes potential solutions for (i) the developing of a more comprehensive procedural framework for the infringement procedure and non-binding regulatory acts; (ii) the strengthening of accountability mechanisms; and (iii) the enhancing of the effectiveness of their application.
Investigation of kinase activation in fibrodysplasia ossificans progressiva
Keywords: ACVR1
Signalling
small molecule inhibitor
Kinase
FKBP12

Fibrodysplasia ossificans progressiva (FOP) is a rare autosomal dominant disease resulting in episodic but progressive extraskeletal bone formation. FOP is caused by missense mutations in the cytoplasmic domain of the type I bone morphogenetic protein (BMP) receptor ACVR1, leading to dysregulated activation. Currently there are no available drug treatments and the structural mechanism of mutant activation is still poorly characterised. To address this, a number of BMP and TGFβ receptors, including FOP mutants of ACVR1 were cloned, expressed and purified for both structural and biophysical experiments. The arginine at the site of most recurrent FOP mutation, R206H, is common across all type I receptors except BMPR1A and BMPR1B which have a lysine at this site. The novel structure of BMPR1B differed to wild-type ACVR1 showing some of the conformational changes expected of the active conformation. However, a variety of disease related ACVR1 mutant structures, including ACVR1 R206H, revealed a surprisingly persistent inactive conformation in the kinase domain. Some conformational changes suggestive of activation were observed in the mutant Q207D affecting the ATP pocket, the β4–β5 hairpin and the activation loop. Additionally, the structure of the Q207E mutant showed a slight release of the regulatory glycine-serine rich domain from its inhibitory position. These subtle changes suggest that the mutant inactive conformation is destabilised and potentially more dynamic. In agreement, all of the ACVR1 mutants showed reduced binding to the inhibitory protein FKBP12. However, mutant phosphorylation of the substrate Smad1 was not constitutive, but dependent on the co-expression of the partner ACVR2, consistent with recent evidence from transgenic knock-out mice. A novel 2-aminopyridine inhibitor scaffold with favourable specificity for ACVR1 was identified using a fluorescence-based thermal shift assay. Further derivatives were characterised with improved potency and selectivity. The crystal structures of ACVR1 bound to these inhibitors showed exquisite shape complementarity, contributing to their favourable specificity. This work has increased the understanding of FOP-associated mutant activation and provided a novel starting scaffold for potential drug development.
The emotional voter: the impact of electoral campaigns and emotions on electoral behaviour in Britain
Keywords: voting behaviour
emotions
turnout
electoral campigns

This thesis examines the role of emotions in mediating the effects electoral campaigns have on political behavior in Britain. I contend that electoral campaigns, aside from direct effects, can also have indirect effects, manifested through the impact of the emotions they induce. I theorize that, through manipulating the tone, framing and targeting of their messages, electoral campaigns induce specific emotions. Emotions are argued to have a direct effect on turnout (intentions) and a moderating effect on the impact partisanship, policy preference and leader evaluations have on vote choice.
Extending the Theory of Affective Intelligence, I hypothesize that individuals who are enthusiastic about their preferred party, or experience anxiety or anger in relation to an out-party, are more likely to turn out, and to cast their vote based on their partisanship. Contrarily, anxiety and anger experienced towards the preferred party are expected to decrease the importance of partisanship and increase the relevance of policy preferences and leader evaluations when voting. While anger experienced towards this party is also hypothesized to also decrease turnout, anxiety is not thought to affect it.
To test these propositions, I rely on a multi-methodological approach that uses both panel and experimental data. The panel data was collected in two waves prior to the 2010 British General Election. The laboratory experiment, designed to specifically test the emotion-induction capacity of campaigns, was conducted on British participants in the aftermath of the same elections. The results corroborate the theory. First, the analyses confirm that campaigns, not only can, but actually do induce emotions. Second, it is shown that emotions do influence political behavior as expected. Third, it is established that the effect of the campaign on turnout intentions is partly channeled through emotions. Finally, it is shown that campaign exposure indirectly affects vote choice by increasing the magnitude of the impact emotions have on the effect of partisanship on vote choice.
Aside from the literature on campaign effects in Britain, the thesis also contributes to the emerging literature pertaining to the role of emotions in politics. Moreover, it contributes to the field of voting behaviour by extending our understanding of the psychological underpinnings of vote choice.
Law, government and authority in mid-Tudor England 1540-1570
Keywords: Sixteenth Century England
Law
Goverment
Authority

This thesis investigates legal and political authority in mid-Tudor England, using the operation of the legal system in Oxfordshire and Berkshire in the period from 1540 to 1570 as a case study through which to give context and content to more abstract forms of contemporary political thought. It seeks to address the broad question: how did people experience the civil—as opposed to the criminal—legal system, and what does this tell us about their understanding of authority? In so doing, it aims to shed light on mid-Tudor attitudes towards legality, limited government and the rule of law.
The argument of the thesis is built up piece by piece. First, people in the Thames Valley engaged in a remarkable, and increasing, amount of litigation over the period in question, much of which was based on antecedent commercial transactions. Encountering the law, whether in its litigious or transactional forms, was thus a very common social experience. Second, despite their frequent interaction with the legal system, ordinary people perhaps knew less about the technical law than some historians have suggested. Third, even if technical legal knowledge was not widespread among the population, the complicated map of overlapping jurisdictions and rival legal institutions meant that ordinary people nonetheless cultivated a detailed mental map of their legal world. Boundaries mattered because jurisdiction and legal authority mattered. Fourth, partly because of their own lack of detailed technical knowledge, and partly because of the complicated legal landscape which they had to navigate, people sought out legal advice from professional advisers. Fifth, even if people frequently sought out professional legal advice, many people’s experience of the law (and of lawyers) was far more negative than their desire to seek out such advice might suggest. Seen in this light, the social utility of legal institutions was quite separate from their ability to provide an idealised standard of abstract justice.
King, cities, and elites in Macedonia c. 360-168 BC
Keywords: Greek history
king
ancient history
Macedonia
settlement

This thesis investigates the nature of the relationship between cities and king in the late Classical and Hellenistic Macedonian kingdom. It will consider the cities from two main perspectives: the city as a community, and the city as a settlement. Section 1 re-examines the evidence most commonly used to argue for the Macedonian cities gaining substantial autonomy in this period. It will be argued that this evidence has less to tell us about the political autonomy of the Macedonian cities than their 'social relations' with other Greek communities: Macedonian cities engaged in international exchanges which did not represent any challenge to the authority of the monarch, but which could also be used to represent the relationship between king and city as cooperative. Such latitude was balanced, however, by forceful expressions of royal dominance in other arenas. Section 2 considers the position of the cities within the royal economy, and examines how, as a result of the king's monopolisation of Macedonia's resources, and the fact that the Macedonian elite was more interested in advancing their position at court than acting as civic benefactors, the cities were left economically subordinated to the king. Section 3 uses the increasingly abundant archaeological evidence to consider how royal building programmes served to project royal ideology into the localities. Royal palaces, large-scale urban development, and fortifications created an experience of urban space in Macedonia which emphasised the roles of the monarch as guardian, benefactor, and unifying figure. The picture that emerges is of a kingdom of civic communities which were engaged in meaningful exchanges with their peers outside Macedonia, but which were living in large and impressive urban settlements which stood as monuments to the extent and ubiquity of royal authority. Late-Classical and Hellenistic Macedonia was a kingdom of poleis, but that kingdom was first and foremost a royal space.
Dissecting tunicamycin biosynthesis: a potent carbohydrate processing enzyme inhibitor
Keywords: glycosyltransferase
epimerase
Streptomyces
biosynthesis
Tunicamycin
antibiotic
natural product

Tunicamycin nucleoside antibiotics were the first known to target the formation of peptidoglycan precursor lipid I in bacterial cell wall biosynthesis. They have also been used extensively as inhibitors of protein N-glycosylation in eukaryotes, blocking the biogenesis of early intermediate dolichyl-pyrophosphoryl-N-acetylglucosamine. Despite their unusual structures and useful biological properties, little is known about their biosynthesis. Elucidating the metabolic pathway of tunicamycins and gaining an understanding of the enzymes involved in key bond forming processes would not only be of great academic value in itself, it would also unlock a comprehensive toolbox of biosynthetic machinery for the production of tunicamycin analogues which have the potential to act as novel therapeutic antibiotics or as specific inhibitors of medicinally important NDP-dependent glycosyltransferases.
I – Cloning the tunicamycin biosynthetic gene cluster. We report identification of the tunicamycin biosynthetic genes in Streptomyces chartreusis following genome sequencing and a chemically-guided strategy for in silico genome mining that allowed rapid identification and unification of an operon fractured across contigs. Heterologous expression established a likely minimal gene set necessary for antibiotic production, from which a detailed metabolic pathway for tunicamycin biosynthesis is proposed.
II – Natural product isolation and degradation. We have developed efficient methods for the isolation of tunicamycins from liquid culture in preparative quantities. A subsequent relay synthesis furnished advanced biosynthetic intermediates for use as precursors in the production of tunicamycin analogues and as substrates for the in vitro characterisation of individual Tun enzymes.
III – Functional characterisation of tun gene products. Individual tun gene products were over-expressed and purified from recombinant E. coli hosts, allowing in vitro functional studies to take place. An NMR assay of biosynthetic enzyme TunF showed it acted as a UDP-GlcNAc-4-epimerase. Putative glycosyltransferase TunD showed hydrolytic activity towards substrate UDP-GlcNAc but failed to accept to the expected natural acceptor substrate, providing unexpected insights into the ordering of biosynthetic events in the tunicamycin pathway. Initial studies into the over-expression of the putative sugar N-deacetylase TunE were also described.
IV – Towards synthesis of tunicamycin fragments. Investigations into a novel synthesis of D-galactosamine – a structural motif within tunicamycin – led to the unexpected observation of inverted regioselectivity upon RhII-catalysed C-H insertion of a D-mannose-derived sulfamate. This was the first example of N-insertion at the beta- rather than gamma-C-H based on conformation alone and warranted further investigation. The X-ray structure of a key sulfamate precursor offered valuable insights as to the source of this unique selectivity.