VictorJuiz/thesis huggingface dataset:
The Use of Eye Movements as an Objective Measure of Mind Wandering
Keywords: Mind wandering
Eye movement behaviour
Reading

Previous research on mind wandering has used subjective verbal reports as a means to acquire the frequency and rate of its occurrence. I proposed the use of eye movements as an objective measure of mind wandering while participants attempted a reading task. Participants were placed in a self-classified probe-caught mind wandering paradigm while their eye movements were recorded. They were randomly probed every 2-3 minutes and were required to indicate whether their mind had been wandering before they were allowed to continue reading. The overall pattern showed that eye movement behaviour was slower and less frequent when participants reported mind wandering episodes, with duration and frequency of within-word regressions reaching levels of statistical significance. It may be that the cognitive processes that normally guide eye movements during reading exert less control during mind wandering episodes. Other implications and limitations are discussed.
The Effect of Rapid Palatal Expander Appliances on Speech
Keywords: palatal expander
speech
adaptation

Background: Rapid palatal expanders have a screw that covers the palate and may affect speech. Methods: RPEs were treatment planned for 22 patients. Speech recordings were completed at 6 different time points. Analysis for speech acceptability, /i/ vowel formants, and /s/ and /∫/ fricative spectra were analyzed. Results: When the appliance was inserted, speech acceptability deteriorated but improved over time. For vowel /i/, the first formant increased and second formant decreased. For fricatives (/s/, /∫/), low to high frequency ratios indicated that the sounds were distorted when the appliance was inserted. The formants and ratios returned to normal levels over time. Examination of the four spectral moments found the spectral mean decreased, standard deviation increased, skewness became more positive, and kurtosis decreased at appliance insertion. Repeated measures ANOVAs found significant effects for time for all acoustic measures. Conclusions: Speech was altered when the appliance was inserted, but improved over time.
Rapamycin-induced Allograft Tolerance: Elucidating Mechanisms and Biomarker Discovery
Keywords: Transplantation
Tolerance
Regulatory T Cells
Rapamycin
Genomics
Biomarkers

The long-term success of transplantation is limited by the need for immunosuppression; thus, tolerance induction is an important therapeutic goal. A 16-day treatment with rapamycin in mice led to indefinite graft survival of fully mismatched cardiac allografts, whereas untreated hearts were rejected after 8-10 days. Specific tolerance was confirmed through subsequent skin grafts and in vitro lymphocyte assays that showed recipient mice remained immunocompetent towards 3rd party antigens but were impaired in responding to donor antigens. Mechanisms that account for this tolerant state were then investigated. Splenic CD8+CD44+ memory T-cells were reduced in tolerant mice but had increased frequencies of the CD62LLO population. CD4+CD25+Foxp3+ regulatory T-cells were increased in tolerant mice. Through multiplex PCR, 4 regulatory T-cell related genes were found up-regulated and 2 proinflammatory genes were down-regulated in accepted hearts. This expression pattern may serve as a putative biomarker of tolerance in patients undergoing transplantation.
Towards a Surface Microarray based Multiplexed Immunoassay on a Digital Microfluidics Platform
Keywords: Digital microfluidics
Immunoassay

The use of digital microfluidics (DMF) for sample handling in a microarray immunoassay was investigated. A two plate DMF device was used, with the top plate being used for the immobilization of antibodies for a sandwich immunoassay. A patterning procedure was developed for the top plate to expose patches of glass that were chemically modified, using silane chemistry, to allow for the covalent immobilization of antibodies. For creating microarrays, a set of parallel microchannels were used for the high density patterning of proteins onto the functionalized surface of the top plate. This patterning procedure was optimized to ensure the reproducibility of the immobilization and the physical integrity of the top plate. Preliminary work for a multiplexed immunoassay such as verification of cross-reactivity and detection schemes was also conducted. This work represents the initial efforts towards a microarray immunoassay on DMF, which has the potential to improve high throughput analysis.
Thermal Spray Forming of High-efficiency Metal-foam Heat Exchangers
Keywords: heat

Thermal spray coating processes have been employed in the current study to deposit well-adhered, dense skins onto the surfaces of open-cell metal foams. The result is a channel that consists of a metal foam core and a thermal sprayed skin wall that can be used as a compact heat-exchanger by directing the coolant flow through the foam. To study the feasibility of the metallic foam heat-exchangers, hydraulic and heat-transfer characteristics were investigated experimentally. The local wall and fluid temperature distribution and the pressure drop along the length of the heat exchanger were measured for different coolant flow velocities. The Dupuit-Forchheimer modification is employed with the experimental results to determine flow characteristics. To measure the heat transfer performance, a length average Nusselt number is derived from a volumetric heat transfer coefficient based on the local wall and fluid temperatures. Heat transfer was shown to have increased nearly 7 times compared to that of a channel without a foam core.
Computational Fluid Dynamics Modeling of Redundant Stent-graft Configurations in Endovascular Aneurysm Repair
Keywords: endovascular
aneurysm
computational fluid dynamics
CFD
EVAR
redundancy
stent-graft

During endovascular aneurysm repair (EVAR), if the stent-graft device is too long for a given patient the redundant (extra) length adopts a convex configuration in the aneurysm. Based on clinical experience, we hypothesize that redundant stent-graft configurations increase the downward force acting on the device, thereby increasing the risk of device dislodgement and failure. This work numerically studies both steady-state and physiologic pulsatile blood flow in redundant stent-graft configurations. Computational fluid dynamics simulations predicted a peak downward displacement force for the zero-, moderate- and severe-redundancy configurations of 7.36, 7.44 and 7.81 N, respectively for steady-state flow; and 7.35, 7.41 and 7.85 N, respectively for physiologic pulsatile flow. These results suggest that redundant stent-graft configurations in EVAR do increase the downward force acting on the device, but the clinical consequence depends significantly on device-specific resistance to dislodgement.
"Nanomedicine: Governing Uncertainties"
Keywords: Nanomedicine
law and risk in novel biotechnologies

Nanomedicine is a promising and revolutionary field to improve medical diagnoses and therapies leading to a higher quality of life for everybody. Huge benefits are expected from nanomedicine applications such as in diagnostic and therapeutic field. However, nanomedicine poses several issues on risks to the human health. This thesis aims to defense a perspective of risk governance that sustains scientific knowledge process by developing guidelines and providing the minimum safety standards acceptable to protect the human health. Although nanomedicine is in an early stage of its discovery, some cautious measures are required to provide regulatory mechanisms able to response to the unique set of challenges associated to nanomedicine. Nanotechnology offers an unique opportunity to intensify a major interplay between different disciplines such as science and law. This multidisciplinary approach can positively contributes to find reliable regulatory choices and responsive normative tools in dealing with challenges of novel technologies.
Temporal De-biasing of Behaviour in Residential Energy Consumption: Supporting Conservation Compliance Through Feedback Design
Keywords: Feedback
Energy Conservation
Temporal Construal Theory
Conservation and Demand Management
Compliance

Despite years of research in residential energy conservation, means of inducing conservation behaviour through feedback are not well understood. In this thesis I take a novel approach to feedback design by addressing temporal inconsistencies that may hinder individuals from forming an intention to conserve. To help understand conservation compliance strategies, I proposed a visual framework to categorize interventions. I present two design heuristics that were inspired by temporal construal theory (Liberman & Trope, 2003). They were the impetus for the design of three feedback display prototypes, which were examined. Due to methodological limitations, significant improvements to compliance were not found. However, evidence suggests that comparative feedback may have supported reasoning about conservation rather than supporting conservation compliance directly. Future work includes refinement of feedback displays to avoid direct comparisons, exploring the use of nature imagery, and the study of a possible interaction between environmental values and comparative feedback on compliance.
Kerr v. Danier Leather: an Analysis of the Difficulty to Enforce a Duty to Update Statements about the Future in the Context of Securities Regulation
Keywords: forecast, securities, liability, misrepresentation, duty to update

Forecasts, predictions and opinions about the future should not be treated in the same way as hard information is treated under the Securities Act. Because this type of soft information cannot be verified in advance, the imposition of liability in respect of these statements about the future may hinder their production and have a result that is adverse to the interests of investors – who would prefer to hear management speak candidly about its thoughts on the company’s future performance. This essay examines the way in which the Ontario Securities Act treats statements about the future, as well as the most important decision in this area up to the present: Kerr v. Danier Leather. It will also discuss whether there should be a duty to update predictions when the circumstances that formed the basis of these forecasts have changed significantly.
Mother? A Portrait of Legal Motherhood in Canada
Keywords: mother
motherhood
sexuality
Canada
legal motherhood

What characteristics make a legal mother? The thesis explores some of the gendered differences in establishing legal parentage. It defends that there is no adequate conception or definition of legal motherhood in Canada. Indeed, the conception of legal motherhood is elusive or reiterates the problematic association between biological motherhood and legal motherhood. The logical leap between a biological situation and a legal status creates two main problems. First, the elusiveness of motherhood as a legal category tends to strengthen gendered assumptions in legal parentage, and it is especially burdensome on women. Second, given the fact that no guidance is provided to decision makers, the vesting of motherhood is often subjected to an evaluation of a woman’s sexuality, sexual choices, or sexual preferences. There is a need a to put forward a comprehensive analysis of legal parentage; a gender and sexuality neutral concept of stratified legal parenthood.
On the Minimization of Regulatory Margin Requirements for Portfolios of Financial Securities
Keywords: Margin Requirements
Capital Charge
Margin Regulations
Margin Account
IIROC

A margin account is a type of brokerage account that allows investors to buy and sell financial securities using credit. The account’s margin requirement is the amount of collateral required, from the investor, to cover the funds or securities extended by the broker to the investor. In Canada, the primary driver of an account’s margin requirement is the account’s Capital Charge [CC] which is calculated using a set of regulatory rules. The regulations are degenerate in that hundreds of valid CCs often exist for a single account. This work outlines a linear optimization model for selecting the minimal CC out of the set of valid CCs for a given margin account. The method proposed is consistent with all of the regulatory requirements and is guaranteed optimal in most cases. Relative to existing methods, the new method produced an average CC reduction of approximately 2% and displayed qualitatively better run-times.
The In vivo effects of Rac1 and Rac2 on Bone Quality and Aging
Keywords: Rac1
Rac2
aging
bone quality

Introduction: The Rho family of small GTPases, including Rac1 and Rac2, are key regulators of osteoclast differentiation and function; however, little is known about their roles in bone quality and aging. Methods: Male four and nine month old mice (n=10) with Rac1, Rac2 or both (DKO) isoforms deleted in osteoclast precursors were assessed using dual energy x-ray absorptiometry (DXA), scanning electron microscopy (SEM), micro computed tomography (microCT), compression, torsion and three-point bending testing, back scattered electron microscopy (BSE), Goldner’s trichrome and TRAP staining. Results: All Rac null mice demonstrated decreased cortical structural properties and improved trabecular architecture. With age, Rac null mice demonstrated the ability to attenuate age-related bone loss. Conclusions: Using an in vivo model with Rac1, Rac2 or both Rac isoforms deleted in osteoclasts, our findings demonstrate the deletion of Rac1 and Rac2 compromised cortical bone while improving trabecular bone properties and attenuated age-related bone loss.
The Brain Coordination Dynamics of Autism: A Study of Auditory Attention
Keywords: Autism
Brain Coordination
Auditory Attention
Phase Synchrony
Rates of Fluctuation

Children and adolescents with autism spectrum disorder (ASD) were compared to age-matched typically developing controls in the context of phase synchrony statistics acquired through magnetoencephalography. Subjects performed a “low-attention” task, pressing a button to a single, repeated auditory tone, and this was contrasted to an “oddball” task where they were required to button press only to deviant tones. The differences in task outcomes were primarily thought to relate to differences in sensory and executive attention. Regardless of task condition, ASD subjects showed greater magnitudes of synchrony between the parietal hemispheres which variably extended across frequencies. Additionally, for ASD subjects, increases in synchrony from the “low-attention” task to the “oddball” task were observed; whereas an opposite tendency occurred for controls. Furthermore, rates of fluctuation of phase differences showed more stable parietal synchrony within ASD across the hemispheric midline. Implications including the possibility of coordination as an indicator of ASD are discussed.
Comparative Analysis between the Canadian Trust and the Panamanian Foundation of Private Interest
Keywords: Foundations of Private Interest
Canadian Trust

Canadian Trusts and Panamanian Foundations of Private Interest are generally utilized as juridical vehicles through which a creator can designate a person to hold and administer property for the benefit and enjoyment of a beneficiary. Given the similarity of application of both vehicles, and taking into consideration that they emanate from separate juridical and judicial systems, it is pertinent to analytically compare them. As can be expected, there is a significant number of aspects through which Foundations of Private Interest and a Trusts can be compared. The following comparative analysis will be centered around three aspects: Asset ownership, creation mechanisms and the requirement of properly identifying beneficiaries. Preceding the aforementioned comparative analysis, a presentation and description of both legal vehicles will be provided in order to make the comparative analysis comprehensible and hopefully useful.
Circulating Unmetabolized Folic Acid: Relationship to Folate Status and Effect of Supplementation
Keywords: Folic acid
Pharmacokinetics

There are increasing concerns that exposure to unmetabolized folic acid, which results from folic acid intakes that overwhelm the liver’s metabolic capacity, may be associated with adverse effects. In this study, we examined the folic acid status of women of reproductive age in relation to dietary intake and the effect of folic acid supplementation (1.1 mg or 5 mg). Plasma unmetabolized folic acid was not significantly correlated with folate intake estimated by food frequency questionnaire or biomarkers. The proportion of women with detectable levels of unmetabolized folic acid increased from 65% to 100% after twelve weeks of supplementation (p < 0.05), however, the increase in concentrations did not reach statistical significance and the effect was not sustained. Moreover, there were no significant differences between the two doses. This suggests that there are mechanisms by which the body adapts to high folic acid intakes to limit exposure to unmetabolized folic acid.
Ginkgo biloba for the Treatment of Vitiligo vulgaris: An Open Label Pilot Clinical Trial
Keywords: vitiligo
Ginkgo biloba

Objective: To conduct an open label clinical pilot trial using Ginkgo biloba in the treatment of vitiligo in Toronto to test the feasibility of recruitment, patient retention, variability of outcome measures, identify safety concerns, and magnitude of treatment effect ahead of a full randomized clinical trial. Methods: 12 participants 12 to 35 years old were recruited to a prospective nonrandomized open-label pilot trial and treated with 60 mg of standardized G. biloba BID for 12 weeks. The primary outcome was the validated Vitiligo European Task Force (VETF). Secondary outcomes included the Vitiligo Area Scoring Index (VASI), photographs, and adverse reactions. Results: Ingestion of Ginkgo biloba was associated with a trend towards improvement on VETF measures of vitiligo lesion area and staging, and significant improvement in VETF spread and total VASI vitiligo measures. Conclusions: By achieving full recruitment, showing benefit, and indicating no adverse reactions the pilot study shows that a future RCT is feasible.
FCHSD2 Regulates Cell Death and Cell Adhesion
Keywords: leukemia
cell death
cell adhesion
F-BAR

FCH/CIP4 homology-Bin-Amphiphysin-Rvs (F-BAR) domain proteins are a subfamily of the Bin-Amphiphysin-Rvs (BAR) superfamily of proteins. They contain unique domains that bind and reshape the phospholipid bilayers of endosomal compartments during endocytosis. Using a functional assay for cell survival, we identified an F-BAR protein, FCH/CIP4 homology and double Src homology 3 domains 2 (FCHSD2), that confers drug resistance. Stable expression of shRNA against FCHSD2 in multiple cell types showed that loss of FCHSD2 sensitized cells to apoptosis by doxorubicin. Silencing of FCHSD2 also enhanced the ability of fibroblasts to grow colonies in culture. Mass spectrometry analysis of FCHSD2 protein complexes identified multiple interacting proteins that are involved in adhesion and endosome trafficking. We identified and confirmed a novel interaction between FCHSD2 and sorting nexin 18 (SNX18), a BAR domain protein that binds to endosomes. Our results suggest that FCHSD2 is involved in regulating cellular adhesion and cell death.
Global Cities and their Response to Climate Change
Keywords: climate change
greenhouse gas emissions
mitigation
adaptation
urban greenhouse gas inventory
strategic mitigation planning
cities
urban development

Decision-makers in cities have realized their pivotal role in addressing climate change, and they are responding accordingly. This thesis presents three papers that explore the process of responding to climate change in cities, highlighting the situation in selected global cities with varying economies and development priorities. The methodology for conducting an urban greenhouse gas inventory in three Chinese cities is detailed in the first paper, illustrating issues of economic development and climate change mitigation in a transitional economy. Next, the greenhouse gas emissions savings of various strategic mitigation plans are quantified for Toronto, demonstrating the aggressive actions needed in developed cities to approach carbon neutrality. The third paper explores issues associated with climate change in three developing cities, emphasizing the need for synergic development incorporating strategies for both mitigation and adaptation. The thesis concludes with an overview of the importance of innovation and further research to future responses to climate change.
The Impact of Neighbourhood Density on the Energy Demand of Passive Houses and on Potential Energy Sources from the Waste Flows and Solar Energy
Keywords: Passive House, Community Energy, net-zero energy

This study demonstrates how the density of a neighbourhood affects its energy demand, metabolism (energy and material flows) and its ability to produce its own energy. Single-family detached houses and row townhouses were each modeled using passive solar housing guidelines with the DesignBuilder building energy simulation software. Energy demand is then modeled within neighbourhoods at two densities based on south facing windows fully un-shaded at 9:00 am, and 12:00 pm solar time on Dec. 21. The neighbourhood metabolisms were then calculated based on location and density. The potential energy supply was evaluated from the spatial characteristics of the neighbourhood (for solar) and the metabolism (municipal solid waste and wastewater flows.) The potential energy demand and supply are then compared for the varying building types and densities to determine the sensitivity of the energy supply and demand relationships.
Hha and YdgT Act Through H-NS to Repress Horizontally Acquired Genes
Keywords: H-NS
Salmonella

The bacterial protein H-NS acts to silence horizontally acquired genes. H-NS physically interacts via its N-terminus with two paralogous proteins, Hha and YdgT. Deletion of hha and ydgT results in derepression of a subset of H-NS repressed genes. I compared expression of hha/ydgT-dependent genes in Salmonella strains lacking hns and hha/ydgT/hns. Deletion of all three genes does not result in greater gene expression than deletion of hns alone, indicating that Hha and YdgT cannot act to repress genes in the absence of H-NS. Further, I used site-directed mutagenesis to generate H-NS proteins incapable of binding Hha. Complementing an hns deletion with an Hha-blind H-NS molecule, H-NS I11A, recapitulated the pattern of gene expression in the hha/ydgT strain. Indicating that elimination of the Hha-H-NS interaction is sufficient to result in derepression of hha/ydgT repressed genes. Hha and YdgT repress gene expression by acting through H-NS and cannot act independently of H-NS.
Activated HH Signaling: Deleterious Lineage-dependent Effects on Nephrogenesis and Collecting Duct Formation
Keywords: Kidney, Hedgehog, Patched, Mesenchyme, UPJ, Gli, HH, Nephrogenesis

Hedgehog (HH) signaling controls renal development. Mutations in PTC1, the HH receptor, cause cancer in non-renal tissues. We hypothesized that constitutively active HH signaling is deleterious to renal development in mice with PTC1 deficiency targeted to the metanephric mesenchyme (MM)(Rarb2-Cre;Ptc1 loxP/-, termed Ptc1 mutants). Increased HH signaling in MM of mutant mice was confirmed by qRT-PCR for Ptc1. A decrease in NCAM-positive nephrogenic precursors at E13.5 and WT1-positive glomeruli at E18.5 was found. Increased cortical expression of Foxd1 was observed. At E13.5, a cluster of ectopic cells expressing Raldh2, Ptc2 and Bmp4 accumulated at the presumptive uretero-pelvic junction (UPJ). Magnetic resonance imaging demonstrated an increase in pelvic volume. Constitutive expression of GLI3 repressor via the Gli3Δ699 allele in Ptc1 mutants increased nephron number comparable to wild type mice and decreased pelvic volume compared to Ptc1 mutants. Thus repression of HH activity is required for proper nephrogenesis and patterning of the UPJ.