VictorJuiz/thesis huggingface dataset:
Model testing of foundations for offshore wind turbines
Keywords: offshore
sand
foundation

Suction caissons are a new foundation option for offshore wind turbines. This thesis is focussed on the behaviour of suction caisson foundations in sand and in clay during installation, and under subsequent vertical and combined moment-lateral loadings. The research is based on extensive experimental work carried out using model scaled caissons. The analysis of the results allowed the determination of parameters for hyperplasticity models. Model caissons were vertically loaded in loose and dense sands to study in service states and plastic behaviour. Bearing capacity increased with the length of the caisson skirt. The bearing capacity formulation showed that the angle of friction mobilised was close to the critical state value for loose sands and close to those of peak values due to dilation for dense sands. The vertical load increased, though at a lower rate than during initial penetration, after large plastic displacements occurred. A hardening law formulation including this observed behaviour is suggested. In sand the installation of caissons by suction showed a drastic reduction in the net vertical load required to penetrate the caisson into the ground compared with that required to install caissons by pushing. This occurred due to the hydraulic gradients created by the suction. The theoretical formulations of the yield surface and flow rule were calibrated from the results of moment loading tests under low constant vertical loads. The fact that caissons exhibit moment capacity under tension loads was considered in the yield surface formulation. Results from symmetric and non symmetric cyclic moment loading tests showed that Masing’s rules were obeyed. Fully drained conditions, partially drained and undrained conditions were studied. Caisson rotation velocities scaled in the laboratory to represent those in the field induced undrained response for relevant periods of wave loading, a wide range of seabed permeabilities and prototype caisson dimensions. Under undrained conditions and low constant vertical loads the moment capacity of suction caissons was very small. Under partially drained conditions the moment capacity decreased with the increase of excess pore pressure. In clay, vertical cyclic loading around a mean vertical load of zero showed that in the short term the negative excess pore pressures generated during suction installation reduced vertical displacements. The yield surface and the flow rule were determined from moment swipe and constant vertical load tests. The moment capacity was found to depend on the ratio between the preload Vo and the ultimate bearing capacity Vu. Gapping response was observed during cyclic moment loading tests, but starting at smaller normalised rotations than in the field. The hysteresis loop shape obtained during gapping cannot be reproduced by means of the Masing’s rules.
Cross-cultural poetics in Kateb, Salih, Djebar and Dib
Keywords: Edouard Glissant
Kateb Yacine
postcolonial literature
cross-cultural
Mohammed Dib
francophone literature
Assia Djebar
poetics
Tayeb Salih

The present study elaborates a poetics of cross-cultural writing. Its primary theoretical reference is the ‘cross-cultural poetics’ (poétique de la relation) of Edouard Glissant: a set of poetic tropes and narrative structural strategies that he identifies in the mixed cultural setting of the Caribbean, in Le Discours antillais.
My thesis argues that if these poetic strategies are indeed a response to specific social, cultural and political situations, then if analogous situations were considered elsewhere, we might expect an analogous poetics to arise. Taking North Africa as an example context, and specifically the novels of the Algerians Kateb Yacine, Assia Djebar, Mohammed Dib, and the Sudanese Tayeb Salih, I argue that these writers’ complex poetic strategies engage with – indeed, help to articulate – analogous socio-political concerns arising in their homelands.
The formal poetical analysis of these authors is based on several key thematic tropes and structural strategies that Glissant advocates in his cross-cultural poetics. My five chapters consider roots and origins, living landscapes, silence and screams, literary opacity, and structural polyphony. They also develop a new critical vocabulary to describe how Glissant’s poetical strategies might take form at a close textual level; my analysis reveals a complex, and reciprocal, relationship between poetic expression and socio-political context.
Glissant’s work is therefore shown to be more broadly relevant, but the founding tenets of his theory are also interrogated and questioned; the comparison with a North African setting entails a (re)assessment of the underlying conceptions of Glissant’s poetics – of the implicit logic by which he connects poetic form to social, cultural and political factors. These factors, for Glissant, also display a clear overlap with the (post)colonial; in studying cross-culturality, the postcolonial, and the poetics engendered by their overlapping, my thesis presents a specific critical focus for the postcolonial literary field.
Cohabitation and convivencia:  comparing conviviality in Casamance and Catalonia
Keywords: difference
everyday life
ethnic diversity
migration
Southern Europe
West Africa
transnationalism
Casamance
conviviality
multilingualism
cosmopolitanism
Spain
religious diversity
Senegal
Catalonia

This thesis explores conviviality, a set of processes surrounding everyday living with difference. Based on 18 months of fieldwork (2007-2010) equally split between Casamance, Senegal, and Catalonia, Spain, the comparison takes the transnational lives of Casamançais and their embeddedness in both local fields into account. Locally, Casamançais often spoke of cohabitation (French) and convivencia (Castilian). Exploring discourses as well as practices related to encounters with difference and everyday socialising, this thesis addresses three questions: (1) How do migrants who come from a context of religious and ethnic diversity manage to make their way within new social contexts of cultural diversity? (2) How do their pre-migration experiences of diversity affect the ways in which they deal with the changing configurations of diversity that they encounter in Europe? (3) How do ways of living together with difference change over time in both sending and receiving contexts due to migration and other concurrent societal transformations?
In four ethnographic chapters, I firstly explore everyday neighbourhood encounters and the centrality of multilingual greeting and temporary gatherings in open spaces for conviviality. A second chapter focuses on cultural and religious festivities and argues that, apart from the political recognition of diversity, the local residents’ sensuous experiences of difference are a crucial dimension of conviviality. Addressing challenges to conviviality, the third chapter engages with the processes of social closure, isolation and homogenisation which reveal alternative ways of living with difference. The fourth ethnographic chapter puts migration-related inequalities centre-stage, showing how conviviality also involves subtle forms of inequality.
Analytically, this thesis suggests that conviviality is not a static conception of sociality, but one that is in-process. I find that socio-cultural differences are permanently negotiated, that ways of dealing with difference are translated between the old and new contexts of diversity, and that discourses and practices of living with difference are continuously (re)produced in everyday interactions. Casamançais perspectives reveal ways of maintaining minimal sociality among local residents who remain different.
Schrift- und Schreibmystik. Christina von Hane
Keywords: mysticism
Virgin Mary
manuscript culture
Middle Ages
female spirituality
Christina of Hane
bridal mysticism
performativity
Christina von Hane
Premonstratensian Order

The subject of my thesis is a little-studied hagiographical work that gives important insights into rewriting processes and their significance in medieval textual culture. The anonymous Life of Christina of Hane, a thirteenth-century Premonstratensian nun from the Palatinate, is an example of bridal mysticism which combines the medieval tradition of the reception of the Song of Songs with hagiographic elements. A codicological and palaeographical analysis of the only manuscript shows it to be a sixteenth-century copy, but the type of mysticism and the theological questions that it discusses suggest that the text was initially composed in the thirteenth century, when Christina is thought to have lived.
The theological and spiritual ideas in the text belong to the wider context of communicating the transcendental within the world. My thesis uses performative language analysis to address the problems of textuality and authorization in the Life of Christina of Hane. It yields new insights into the ways in which this mystical text makes use of hagiographic strategies, how gender and vernacular theology are linked, how liturgical elements support the text’s pragmatic nature, and how somatic spirituality is reflected on an allegorical level in the embodiment of God’s bride. An assessment of three communicative aspects – medial, narrative, and allegorical – highlights the textualization of the mystical experience. The appellative structure of Christina’s text invites the reader to engage with the text.
This study provides the first comprehensive interpretation of the text on Christina of Hane. It compares it to other mystical texts, to a German–Latin prayerbook, and to a fragmentary legend about Mary Magdalene. It challenges existing judgments about Christina’s biography and offers alternative solutions founded in the latest scholarship on female mystical literature.
An assessment of the use of human samples in ancient DNA studies
Keywords: Yersinia
DNA
pestis
ancient
contamination
humans

This thesis addresses gaps that exist in the theory and knowledge of ancient DNA (aDNA). Much of the underlying basis of the field has been neglected in the excitement that followed the first aDNA studies. Therefore the results of many studies have been based on untested assumptions about the nature of post mortem DNA damage, sample preservation, contamination, and the efficacy of sample decontamination techniques. The validity of such results is questionable if the assumptions prove false.
Hydrolytic post mortem DNA damage may modify recovered aDNA sequences. This thesis reports new insights into the biochemical basis of, predisposition of certain sequences and nucleotide positions towards, and subsequent effects of, such damage. Parallels of post mortem damage with in vivo mutation also enable insights into DNA sequence evolution.
The long-term survival of DNA, and contamination of samples with exogenous DNA are two related problems characteristic to aDNA. The survival of endogenous DNA within bone, teeth and hair samples, the susceptibility of such samples to contamination, and the efficacy of decontamination techniques used to remedy such problems are investigated. The results highlight serious flaws in using bone and teeth as a DNA source. In contrast, the results demonstrate that hair may present a valuable DNA source for future studies.
Numerous studies have reported the retrieval of ancient pathogen DNA from human samples. Analyses of the DNA content within teeth extracted from putative victims of the 2nd plague argue that such studies are at great risk from DNA degradation, and contamination arising due to environmental microorganisms. An extrapolation of these results using basic physical and chemical theory is used to evaluate the potential survival of aDNA in ancient Egyptian remains. This suggests that positive results from such samples are unlikely.
Energy transfer processes in supramolecular light-harvesting systems
Keywords: molecular structure
time-correlated single photon counting
resonance energy transfer
condensed matter physics
spherical organic asssembly
DNA-hybrid structure
morphology
molecular wire
time-integrated photoluminescence

This dissertation attempts to understand how energy transfer in a molecular wire and a spherical organic assembly are affected by molecular structure. The molecular wire is a DNA-hybrid structure composed of a strand of thymine bases appended by a cyanine dye. Hydrogen bonded to each base is a naphthalene-derivative molecule. Using time-integrated photoluminescence and time-correlated single photon counting measurements, energy transfer from the naphthalene donors to the cyanine acceptors was confirmed, and its dependence on temperature and DNA-template length investigated. Donor-thymine bonding was disrupted at temperatures above about 25 degrees Celcius resulting in poor donor template decoration and low rates of energy transfer. Increasing numbers of donors attach to the scaffold, forming an orderly array, as the template length increases due to the stabilising effects of the donor-donor pi-stacking interactions. Conversely, modelled energy transfer rates fall as the scaffold length increases because of the longer donor-acceptor distances involved. Therefore, the energy transfer rate was greatest for a template built from 30 thymines.
The spherical organic assemblies (nanoparticles) are formed by fast injection of a small volume of molecularly dissolved fluorene-derivative amphiphilic molecules into a polar solvent. The amphiphilic molecules contained either a naphthalene (donor) or a benzothiadiazole (acceptor) core. The donor-acceptor mixed nanoparticles resemble an amorphous polymer film and were modelled as such using the Foerster resonance energy transfer theory. The Foerster radii extracted from the measurements depends intricately on the donor-acceptor spectral overlap and distance. The latter effect was controlled by the stacking interactions between the molecules.
Altering the morphology of the structural units is the key to optimising energy transfer in molecular structures. To achieve efficient organic molecule-based devices, the importance of this property needs to be fully appreciated and effectively exploited.
Mathematical modelling of flow and transport phenomena in tissue engineering
Keywords: asymptotic reduction
multiphase flow
tissue engineering

Tissue engineering has great potential as a method for replacing or repairing lost or damaged tissue. However, progress in the field to date has been limited, with only a few clinical successes despite active research covering a wide range of cell types and experimental approaches. Mathematical modelling can complement experiments and help improve understanding of the inherently complex tissue engineering systems, providing an alternative perspective in a more cost- and time-efficient manner.
This thesis focusses on one particular experimental setup, a hollow fibre membrane bioreactor (HFMB). We develop a suite of mathematical models which consider the fluid flow, solute transport, and cell yield and distribution within a HFMB, each relevant to a different setup which could be implemented experimentally. In each case, the governing equations are obtained by taking the appropriate limit of a generalised multiphase model, based on porous flow mixture theory. These equations are then reduced as far as possible, through exploitation of the small aspect ratio of the bioreactor and by considering suitable parameter limits in the subsequent asymptotic analysis. The reduced systems are then either solved numerically or, if possible, analytically. In this way we not only aim to illustrate typical behaviours of each system in turn, but also highlight the dependence of results on key experimentally controllable parameter values in an analytically tractable and transparent manner. Due to the flexibility of the modelling approach, the models we present can readily be adapted to specific experimental conditions given appropriate data and, once validated, be used to inform and direct future experiments.
Biochemical characterization of a novel deubiquitinating enzyme otubain 1 and investigation into its role in Yersinia infection
Keywords: host-pathogen interactions
phosphorylation
Yersinia
proteomics
ubiquitin
deubiquitinating enzymes

Deubiquitinating enzymes (DUBs) constitute a diverse protein family. The specificities and functions of the majority of DUBs are unknown, although their impact on many biological and pathological processes is widely appreciated. This dissertation entails a detailed characterization of otubain 1 (OTUB1), an ovarian tumor domain-containing DUB. The presented work describes OTUB1’s specificity, localization, protein interactions, importance in infection with Yersinia, and proposes a novel model of regulation of its enzymatic activity.
I first discuss the structural and biochemical properties of OTUB1, demonstrating its selectivity towards ubiquitin and NEDD8. Moreover, I show that OTUB1 cleaves lys48- but not lys63-linked polyubiquitin, emphasizing its role in ubiquitin-mediated proteasomal degradation. Mass spectrometric identification of interaction partners and localization studies suggest possible involvement of OTUB1 in RNA processing and cell morphology.
Furthermore, I demonstrate that invasion of the host cells by the enterobacteria Yersinia can be altered by changing OTUB1 expression. This effect is dependent on the catalytic activity of OTUB1 and its ability to stabilize RhoA-GTP prior to infection. YpkA and OTUB1 modulate RhoA-GTP stability in opposing ways, leading to cytoskeletal rearrangements that may be involved in bacterial invasion. Moreover, OTUB1 is post-translationally modified by phosphorylation that modulates its ability to stabilize RhoA-GTP and counteracts its effect on bacterial invasion. These findings provide a novel entry point for the manipulation of the host—pathogen interactions.
Lastly, a kinase screen revealed that FER, an oncogenic kinase with a role in cell morphology, phosphorylates OTUB1, as demonstrated by overexpression, siRNA and in vitro studies. The phosphorylated site was mapped to tyr26 and the activity-based labeling revealed that this modification interferes with the deubiquitinating activity of OTUB1.
In summary, the results presented in this thesis confirm that OTUB1 exerts properties of a “classical DUB” and uncover some of its physiological functions.
Investigations of the potential schizophrenia susceptibility gene Kinase Interacting with Stathmin (KIS)
Keywords: schizophrenia
UHMK1
KIS

Single nucleotide polymorphisms (SNPs) within the gene encoding the serine threonine kinase KIS (Kinase Interacting with Stathmin, also known as UHMK1) have recently been associated with schizophrenia. However, little is known about the neurobiology of KIS or the mechanisms through which disease-associated SNPs may increase susceptibility to schizophrenia. The studies presented in this thesis focus on the distribution of KIS and its mRNA, address the mechanisms through which KIS may confer susceptibility to schizophrenia, and investigate the physiological role(s) of KIS in the brain using two lines of knockout mice.
The regional and cellular distribution of KIS was characterised in the brains of adult humans and mice, and through mouse neurodevelopment. The results of these experiments demonstrated that KIS is widely expressed in neurons in the brain regions examined in both species, its encoded protein is localised to the nucleus and cytoplasm, and KIS expression in mouse brain peaked around seven days after birth.
KIS protein and mRNA was quantified in two large human post-mortem brain series to determine if KIS expression is altered in schizophrenia or bipolar disorder, or in relation to the leading schizophrenia-associated SNP (rs7513662). Using tissue from the superior temporal gyrus, dorsolateral prefrontal cortex, anterior cingulate cortex, and cerebellum, no difference in KIS expression (either mRNA or protein) was found between diagnostic or genotype groups in any brain region examined. Furthermore, KIS expression in lymphoblast cell lines also did not differ between diagnostic or genotype groups.
Lastly, KIS expression (mRNA and protein) was characterised in two separate lines of KIS knockout mice. The results were complex, and left uncertainty as to whether either line was a true knockout or, conversely, whether any of the available KIS antibodies were specific. Nevertheless, the absence of KIS mRNA was robustly confirmed in one knockout mouse line, and brains from this line were subsequently used in three experiments. First, investigation of the quantity and phosphorylation state of the KIS targets stathmin and p27kip1; no differences were found compared to wildtype mice. Second, quantification of mRNA expression of several other genes of interest with regard to schizophrenia; altered expression of GAP43, VGlut1, MAP2, spinophilin, and stathmin was found. Finally, stereological measurements were performed, and while no differences in whole or regional brain volumes in KIS knockout mice were found, there was a relative reduction in hippocampal volume.
The results of these studies do not support the hypothesis that altered expression is the mechanism by which genetic variation of KIS may increase susceptibility to schizophrenia, nor that KIS expression is altered in the disease. However, the knockout mice data suggest that KIS may play a role in synaptic plasticity and function, providing novel information on the potential neurobiological roles of KIS.
Deference in international human rights law
Keywords: deference
margin of appreciation
proportionality

Deference in international human rights law has provoked animated discussion, particularly the margin of appreciation doctrine of the European Court of Human Rights. Many commentators describe the practice of deference but do not explain how it affects judicial reasoning. Some approve characteristics of deference but do not provide a justification to defend the practice against criticism. Others regard deference as a danger to human rights because it betrays the universality of human rights or involves tribunals either failing to consider a case properly or missing an opportunity to set human rights standards. This thesis employs a different approach by focussing on deference as the practice of assigning weight to reasons for a decision on the basis of external factors. This approach draws on theories of second-order reasoning from the philosophy of practical reasoning. The thesis offers a conceptual account of deference that accords with the practice not only of the European Court of Human Rights, but also the Inter-American Court of Human Rights and the UN Human Rights Committee. Additionally the thesis presents a normative account of deference, that the role of these tribunals entails permitting a measure of diversity as states implement international human rights standards. Deference in international human rights law then is the judicial practice of assigning weight to the respondent states’ reasoning in a case on the basis of three factors: democratic legitimacy, the common practice of states and expertise. This affects judicial reasoning by impacting the balance of reasons in the proportionality assessment. The account defended in this thesis dispels concerns that deference is a danger to human rights, whilst providing a theory that justifies the practice of the tribunals. The thesis thus provides the contours of a doctrine of deference in each of the three international human rights systems.