VictorJuiz/thesis huggingface dataset:
Functional Electrical Stimulation as a Neuroprosthesis for Sitting Balance: Measuring Respiratory Function and Seated Postural Control in Able-bodied Individuals and Individuals with Spinal Cord Injury
Keywords: neuroprosthesis
functional electrical stimulation
postural control
respiratory function
spinal cord injury
seated postural stability

The level and completeness of spinal cord injury (SCI) above the first lumbar vertebra determine the degree of multi-system impairments including altered respiratory function and decreased capacity to maintain upright posture and seated postural stability in humans. Both systems were studied in able-bodied (AB) subjects and individuals with tetraplegia to explore the potential of functional electrical stimulation (FES) as a neuroprosthesis for seated postural control without compromising respiratory function. Data for AB participants (n=10) indicated higher tidal volumes, greater trunk extensor muscle activity, and different values of seated postural stability in upright sitting compared to slouch sitting. In three case studies of individuals with tetraplegia, surface FES was applied to trunk muscles. Changes in tidal volume, respiratory rate, and seated postural stability were case specific. These studies inform the development of a strategy for non-invasive FES as a neuroprosthesis for sitting balance while preserving respiratory function in individuals with SCI.
Stoichiometric Hydrogenated Amorphous Silicon Carbide Thin Film Synthesis Using DC-saddle Plasma Enhanced Chemical Vapour Deposition
Keywords: Stoichiometric, Hydrogenated, Amorphous, Silicon Carbide, DC-Saddle

Abstract Silicon carbide is a versatile material amenable to variety of applications from electrical insulation to surface passivation, diffusion-barrier in optoelectronic and high-frequency devices. This research presents a fundamental study of a-SiC:H films with variable stoichiometries deposited using novel technique, DC saddle-field plasma-enhanced chemical-vapour deposition, a departure from conventional RF PECVD commonly used in industry. DCSF PECVD is an alternative technique for low temperature large area deposition. Stoichiometric a-SiC:H obtained by fine-tuning precursor gas mixture. Annealing up to 800oC showed no significant change in elemental composition; particularly indicating thermal stability at stoichiometry. Ellipsometry showed wide range of optical gaps whose maximum surpasses values reported in literature. Refractive index measured and change in values studied as function of increasing carbon content in the films. Also attainment of very smooth surface morphology for stoichiometric a-SiC:H films reported. Surface roughness of 1 nm rms demonstrated for films grown at temperature as low as 225oC.
Sex and Strain Differences in Acute Hepatotoxic and Inflammatory Responses to Liver Procarcinogens in the Developing Mouse
Keywords: 4-aminobiphenyl
Aromatic amines
Diethylnitrosamine
Carcinogenesis
Hepatocellular carcinoma
Hepatotoxicity
Inflammation

We previously observed that postnatal exposure of mice to the procarcinogen 4-aminobiphenyl (ABP) produced liver tumors only in wild-type males, while arylamine N-acetyltransferase deficient males and females of either strain were protected. Others have also observed a sex difference in liver tumors in mice using the procarcinogen diethylnitrosamine (DEN). Reasons for these sex and strain differences are unclear, but differences in acute hepatotoxicity and inflammation may be involved. In this thesis we found that neither ABP nor DEN produced overt hepatotoxicity in postnatally exposed mice, and only DEN caused an increase in levels of the pro-inflammatory cytokine interleukin-6 but was not sex-dependent. The lack of sex difference suggests that sex hormone modulation of inflammation following sexual maturation might favour growth of initiated cells in males. However, the lack of detectable inflammation following ABP exposure may be due to localized responses, or that inflammation may be a DEN-specific effect.
Oxidative Stress and Global DNA Methylation in Patients with Bipolar Disorder and an Excellent Response to Lithium
Keywords: Bipolar Disorder
Oxidative Stress
DNA Methylation
Lithium

Correlation between 8-hydroxy-2-deoxyguanosine (8-OHdG) and/or redox imbalance and 5-methylcytosine (5mc) levels was studied in patients with bipolar disorder (BD) and an excellent response to lithium treatment, their affected and unaffected relatives (AR and UR, respectively) and controls. We demonstrated that global methylation was decreased in BD subjects and their relatives compared to controls and remained so after lithium treatment in BD subjects, while UR and AR were no longer different than controls. 8-OHdG levels and Complex I activity did not differ between groups before and after lithium treatment. Finally, glutathione peroxidase activity was higher in relatives of patients before and after lithium treatment and negatively correlated with 5mc levels. The methylation pattern in BD subjects may be specific for BD and that lithium may be involved in regulating global methylation. This study supports the antioxidant properties of lithium through regulation of glutathione system.
The Effects of Substrate Oxidation on Post-exercise Food Intake in Pre-pubertal, Normal-weight Boys and Men
Keywords: Food Intake Regulation
Metabolic Flexibility
Substrate Oxidation
Fat Oxidation
Carbohydrate Oxidation
Energy Balance
Energy Intake

The relationship between substrate oxidation (RER) and food intake (FI) is undefined. This study examined the effects of RER modified by a glucose pre-load (GL), exercise (EX) and GL with EX on, FI and energy balance (NEB) in normal-weight boys (9-12 y) and men (20-30 y). Subjects (15 boys, 15 men) were randomized with treatments of either water or GL followed by either EX or rest. Measures included RER, energy expenditure (EE)(kcal/kg), subjective appetite, FI(kcal/kg) measured at a pizza lunch and NEB (kcal/kg). FI(kcal/kg) was reduced by GL(p < 0.0001), and further decreased with GL ingested prior to EX(p = 0.0254). RER was increased with GL(p < 0.0001) and EX(p = 0.0043), and was higher in men compared to boys (p = 0.007). There was no association between RER and FI(kcal/kg). In conclusion, there was no relationship between RER and FI, suggesting that FI is not affected by substrate oxidation.
An Ultra-wideband Spatial Filter for Time-of-arrival Localization in Tunnels
Keywords: Ultra-wideband
Localization
Time-of-arrival
Tunnel

An ultra-wideband (UWB) spatial filter is proposed to mitigate multipath effects in a one-way, one-dimensional time-of-arrival (TOA) localization system for use inside a tunnel. The spatial filter is a weighted array of judiciously placed antennas and it exploits the fact that electromagnetic waves propagate as modes in a tunnel by selectively extracting these mode(s). The design of several spatial filters is presented alongside vigorous analyses to characterize the localization performance afforded by them in a noisy environment. The filters are evaluated using data from an analytical equation waveguide model, a ray tracer model and measurements. These spatial filters deliver accurate localization estimates across distance and well-designed filters can operate at higher SNRs and further distances than single sensors. Insights into successful spatial filter design are provided and this spatial filtering technique has created a new branch of multipath-aware localization systems.
The Production of Protein Isolates from the Aqueous Extraction of De-hulled Yellow Mustard Flour and Determination of their Functional Properties
Keywords: Yellow mustard
Aqueous extraction
Functional properties
Membrane processing

Two types of protein isolates were prepared from de-hulled yellow mustard flour by aqueous extraction, membrane processing and acid precipitation of proteins at the isoelectric point (IEP 5.5). Their electrophoretic, main functional properties and protein composition were determined. The precipitated and acid soluble protein isolates had 83.0 and 96.0% protein content on a moisture and oil free basis, respectively. The acid soluble protein isolate had comparable functional properties to those of commercially available soybean and other protein isolates. The precipitated protein isolate exhibited less desirable functionality than the soluble isolate, due to its high lipid content (~25%); however, it was still comparable to soybean isolates. Storage temperature had limited effect on lipid oxidation, and hence the stability of the precipitated protein isolate at 25-45ºC. Taste and texture of wieners and bologna prepared with 1-2% of this isolate as binder were comparable to those prepared with soy protein isolates.
Properties and Performance of Photocatalytic Concrete
Keywords: Photocatalytic
Concrete

This research program is focused on the photocatalytic cement with emphasis on evaluating impacts on concrete’s physical, transport and durability properties. The scope of this project is to examine three key aspects of photocatalytic concrete including: a critical review of currently reported applications; an examination of air pollution concentrations in Ontario to assess the potential effectiveness of photocatalytic concrete, with respect to NOx; and an experimental study to characterize the material properties. Research findings revealed comparability between photocatalytic concrete and conventional concrete in mechanical and fluid transport properties. Although photocatalytic concrete resisted rapid freeze and thaw damage very well, in terms of de-icer salt scaling performance, only formed surface performed well and highly variable results for photocatalytic and GU concrete was observed for the finished surface.
MIMO Performance of Low Mutual Performance of Low Mutual Coupling Antennas in Indoor and Hallway Environments
Keywords: Antenna
MIMO
channel capacity
mutual coupling

In this thesis, the 2×2 MIMO performance of several low mutual coupling antennas has been investigated in indoor and hallway scenarios. Three compact antennas intended for mobile applications with low mutual coupling between the input ports are presented in this thesis. To gauge the performances of the three designed antennas, two reference antennas are also used. Channel capacity measurements were conducted in Bahen Center Antenna Lab room 8175 and the Bahen Center 8th floor hallway by using the five antennas as receivers. The antenna spatial location, orientation, line-of-sight and non-line-of-sight situation and richness of multipath effect were considered in the measurements. By averaging the results, it is found that in an indoor environment, low mutual coupling antennas can outperform the reference high mutual coupling antennas especially in higher SNR scenarios. In the hallway environment, low mutual coupling antennas always outperform the reference high mutual coupling antennas due to pattern diversity.
Electrochemical Based Detection of Influenza
Keywords: Nanostructured Micro Electrode
Influenza

Influenza is the infectious agent of the seasonal flu. Flu symptoms from influenza infection are similar to the symptoms caused by bacterial upper respiratory tract infections. This similarity causes the inappropriate diagnosis and prescription of antibiotics, leading to drug resistant bacterial strains. Moreover, the limitations of the current viral detection methods prevent the clinical diagnosis of influenza. The objective of this project is to design a rapid and sensitive influenza diagnostic method based on the highly sensitive Nanostructured microelectrode biosensing assay. The diagnostic method was designed by selecting probe sequences, controlling the quality of the probes and the sensing chips, and optimizing the deposition conditions. This diagnostic method was shown to be capable of differentiating influenza sequences from non-complementary sequences, detecting influenza sequences in the form of ~1000-nucleotide RNA molecules, sensing the target influenza RNA within a complex mixture of cell lysates, and achieving a clinically relevant detection limit.
Possible Modifications to the Accelerated Mortar Bar Test (ASTM C1260)
Keywords: Alkali-Silica Reaction
Accelerated Mortar Bar Test
ASTM C 1260
CSA A23.2-25A

The Accelerated Mortar Bar test (AMBT) is rapid, reproducible, and perhaps the most widely used technique for examining the potential alkali-silica reactivity of aggregates. Unfortunately, this test is often unreliable as it may identify non-reactive aggregate as reactive and vice versa. With the aim of improving the accuracy of AMBT, two modifications to the current procedure were evaluated: 1) the maturity of mortar bars prior to alkali hydroxide exposure and 2) reduction of the storage temperature. The original and modified versions were performed on six aggregates with alkali-silica reactive (ASR) components, and their expansions and ASR classifications were compared. Results show that increasing the maturity had no significant impact on expansions. However, modifying the storage temperature to 60˚C and extending the period of testing to 28 days can be very effective in terms of more reliably identifying the existing falsely identified aggregates.
The Effect of Bisphosphonate Therapy on Neutrophil Function: A Potential Biomarker Preliminary Findings
Keywords: Bisphosphonate
Neutrophils
Osteonecrosis
Biomarker

Bisphosphonate-related osteonecrosis of the jaws (BRONJ) occurs subsequent to intravenous and oral bisphosphonate exposure in a small subset of patients. Evidence of concurrent bacterial colonization at sites of bone necrosis, previous reports of neutrophil-related complications in some patients taking bisphosphonates along with perturbed neutrophil function in bisphosphonate-treated mice suggests an innate immune role in the development of bisphosphonate-related osteonecrosis of the jaws. This study investigates neutrophil function in BRONJ patients to determine if neutrophil functional defects may serve as a potential biomarker for BRONJ susceptibility. Patients with BRONJ and patients beginning intravenous pamidronate were studied. Eighteen patients with BRONJ and five patients beginning pamidronate therapy provided oral and blood neutrophil samples. Neutrophils from the population of patients with bisphosphonate-related osteonecrosis of the jaws and from those post-pamidronate treatment showed lower reactive-oxygen species production. These data suggest that a compromise in neutrophil function may be a potential biomarker for BRONJ susceptibility.
MR Diffusion Measurements of Apoptotic Changes in Tumour Cells
Keywords: imaging
magnetic resonance imaging
cancer
diffusion
apoptosis
medical physics
acute myeloid leukemia
cisplatin

Monitoring treatment efficacy is a large area of cancer research as it can increase the effectiveness of therapy regimens. Diffusion weighted Magnetic Resonance imaging (DWI), allows assessment of tissue microstructure without exogenous contrast agents. In this thesis, two different DWI techniques were used to acquire data from acute myeloid leukemia cells undergoing apoptosis, and data was fitted to an analytical model of re- stricted diffusion. Results indicated a decrease in average restriction size from 6.4 to 2.7μm, and an increase in the restricted diffusion coefficient from 0.17 to 0.82μm^2/ms in untreated versus treated cells. The free diffusion coefficient was constant indicating changes in restrictions, rather than any intrinsic changes in the intra-cellular or extra- cellular fluid. This combination of techniques has the potential for use in preclinical and clinical settings as it demonstrates that apoptotic changes may be measured consistently.
Development of Synthetic Processes and Characterization of BsubPcs with High Crystal Densities for Application in Organic Photovoltaic Devices
Keywords: subphthalocyanine
boron
organic
photovoltaic
halo
dimer
oxygen
bridge
bridged
synthesis

The original goal of this thesis was to develop process chemistry to yield boron subphthalocyanine (BsubPc) derivatives which were previously difficult to access. Retrospectively, it was found that these compounds show extremely high density crystal packing in comparison to other known BsubPcs, and thus this also became a focus of the thesis. A process to synthesize and purify fluoro-BsubPc was developed. This led to a detailed comparison of the physical and chemical properties of the three halo-BsubPcs in order to answer the question of which halo-BsubPc is appropriate for different purposes. Through this work, the previously unpublished crystal structure of the oxygen bridged dimer, µ-oxo-BsubPc, was found. A process was subsequently developed for the practical synthesis of µ-oxo-BsubPc for use in vacuum deposition and a number of µ-oxo-BsubPc crystal polymorphs were found and analyzed. The properties of this group of compounds are discussed in the context of other known BsubPcs.
Goal Management Training: A Web-based Approach
Keywords: cognitive rehabilitation
cognitive psychology

This study was undertaken to introduce an innovative approach to cognitive rehabilitation intervention (Goal Management Training- GMT) delivery, through a web-based platform administered to adults with cerebrovascular disease (CVD) or suffering from CVD risk factors who presented with executive function impairments. The feasibility of this approach was investigated by developing a semi-quantitative-qualitative tool to measure therapist competence and group engagement adapted from the Cognitive Therapy adherence-to-protocol scale. Results from two raters analyzing random web-based GMT session recordings showed no compromise in any aspect measured. In addition, the efficacy of the intervention was established using neuropsychological and functional outcome measures, with significant results observed on the Goal Attainment Scale functional measure. To our knowledge, this study is the first of its kind to a) employ videoconferencing technology to overcome accessibility barrier to cognitive rehabilitation and b) develop an adherence to protocol tool to measure various aspects of GMT.
On Mesh Convergence and Accuracy Behaviour for CFD Applications
Keywords: engineering
aerospace
mesh
topology
aeronautical
aircraft
aerofoil
convergence
accuracy
adjoint method

Computational Fluid Dynamics (CFD) is a main field that contributes to the development of high efficiency aircraft. CFD accuracy depends on the flow solver and the meshing of the geometry, and while it is doable to determine why a certain solver is more accurate than another, it is much more difficult to discern why two meshes produce differently accurate solutions. A framework is presented to evaluate the performance or ``goodness" of a mesh and to compare meshes. The framework is composed of quantifiable mesh parameters which define a mesh, and three performance measures: functional accuracy, their order of convergence, and their behaviour under the adjoint correction method. Although it seems that the relationships between parameters and results are not trivial, there are trends from which optimal mesh parameters are deduced. The H topology performs best, and the most important parameters are related to spacings and cell quality around the aerofoil leading edge.
Microfluidics for Steam Assisted Gravity Drainage and Petroleum Applications
Keywords: Microfluidics
Steam Assisted Gravity Drainage

Petroleum research is responsible for making previously unusable reservoirs economically viable and for limiting the environmental impact of petroleum development. Microfluidics, the study of fluid flow at the micro-scale, is predominantly used to study biological phenomena. Adapting microfluidics to study petroleum requires the use of materials and methods not commonly used. This thesis focuses on applying microfluidics to the study of petrochemical fluid flow at high-pressure and high-temperature. The first problem addressed is the study of fluids during alkaline steam assisted gravity drainage (alkaline SAGD). The addition of an alkaline additive is found to improve the bitumen production rate by 35-67%. Secondly, two high-pressure, high-temperature, solvent resistant microfluidic interfaces for glass chips are designed to be operated up to 140 bar and 70 degrees Celsius. Lastly, a novel method for fabricating solvent resistant microfluidic devices from Teflon film is demonstrated by measuring the viscosity of toluene-heavy oil blends.
Rational Design of sym-Triazines For Multitarget-directed Modulation of Cholinesterases and Amyloid-beta in Alzheimer’s Disease
Keywords: Alzheimer's disease
amyloid-beta
acetylcholinesterase

Alzheimer’s disease (AD), a progressive age-related neurodegenerative disorder is characterized by impairments in memory and cognitive functions. The two main pathogenic hallmarks associated with the progression of this multifactorial disease include accumulation of amyloid-beta (Aβ) plaques and the deterioration of the cholinergic system in the brain. Using cost-effective synthetic procedures, mono-, di-, and tri- substituted sym-triazine derivatives incorporating acetylcholine substrate analogues and aromatic phenyl moieties were synthesized for the targeted modulation of Aβ aggregation and acetylcholinesterase (AChE) activity. A subset of these sym-triazines demonstrated dual inhibition of Aβ fibrillization and AChE hydrolytic activity in vitro studies. These highly effective compounds were also shown to be well tolerated by differentiated human neuronal cells in cell viability tests. These novel compounds have the potential to undergo future in vivo pharmaceutical analysis and have a positive impact on the quality of life of the people living with this devastating disease and their caretakers.
Saprolite Leaching and Iron Control in Concentrated Magnesium Chloride Brines
Keywords: Saprolite
Magnesium Chloride
Atmospheric Leaching
Nickel

MgCl2 brines present a number of potential advantages for the processing of saprolite ores for nickel production. Concentrated MgCl2 solutions enhance the activity of acid used, allow atmospheric leaching at elevated temperature and inhibit magnesium dissolution, which reduces acid consumption and increases metal selectivity. However, with a chloride system it is economically requisite to recover hydrochloric acid, conventionally accomplished by pyrohydrolysis. This work was performed in conjunction with a novel flowsheet for the processing on saprolite ores, which recovers HCl by the precipitation and subsequent decomposition of magnesium hydroxychlorides, alleviating some of the issues with pyrohydrolysis. Leaching and iron control experiments have been conducted in concentrated MgCl2 brines, up to 4.5 m, to determine the most amenable process conditions. It was determined that > 95% extraction of metals was possible using both aqueous and gaseous HCl. In addition, the feasibility of iron control by precipitation with MgO addition was proven.
Insulin Receptor Signaling is Necessary for the Maintenance of Epithelial Phenotype in MCF10A Cells
Keywords: Breast Cancer
Insulin
Cell Signalling
Obesity
Type 2 Diabetes

Obesity is an adverse factor in the development and severity of breast cancer. Obesity is often accompanied by an increase in circulating insulin, which is also associated with poor BC prognosis. Although not expressed in normal breast tissue, the insulin receptor (IR) is highly expressed in BC, therefore insulin signaling in BC cells may be responsible for the negative prognostic effects associated with hyperinsulinemia. This thesis describes the development of a cell-based system to study how insulin affects BC. My work shows that MCF10A, untransformed human breast epithelial cells that express the IR, require insulin for normal proliferation and morphology. Interestingly, I discovered hyperactivation of ERK1/2 in MCF10A cells in response to insulin withdrawal, resulting in a loss of epithelial phenotype. Unexpectedly, while losing epithelial phenotype, MCF10A cells depleted of insulin failed to migrate. In conclusion, breast cells that express IR require insulin for migration and maintenance of epithelial characteristics.