VictorJuiz/thesis huggingface dataset:
Politics of Diaspora
Keywords: Diaspora and modernity
race and ethnicity
culture and subjectivity
nation-state and citizenry

The intention of the study is to come into a better understanding of the way in which the Diasporic body comes to know and understand its subjectivity within the governing contemporary public sphere. I suggest that this knowledge is diverse and that it can assist us to re-conceptualize learning in the context of schooling and education. I am interested in this seemingly mundane thing of ‘blackness’ and the way in which the signifying power of ‘blackness’ has come to constitute the conditions of possibility for the formation of a certain humanism. I trace somewhat abstract historical trajectories in order to better understand how contemporary everyday Diasporic life comes to be classified, organized, self-regulated and inscribed through particular intersections of race by way of gender, ableism, class, and sexuality. I seek to ascertain ways in which race is interpreted as the ‘Truth’ in order to impute the ethic of colonialism onto the Diasporic body. With this study my interest concerns understanding my lived experiences within the context of Diaspora and about how I come to make sense of race/racism/blackness through the cultural location of the colonial West. I am seeking to understand how, at certain moments, abject bodies of the Diaspora become predisposed to socialize in specific ways through these protean subjectivities. My interest involves coming to know critical pedagogies immanent to African Diasporic spaces that are germane for re-imagining schooling and education. I am interested in the school as a Diasporic space, the pedagogical and instructional implications for the teacher/educator, and about the ways in which meaning is made of Diaspora. I am suggesting writing Diaspora for schooling and education presents alternative ways of making sense of one’s subjectivity, citizenry, identity, about coming to know and understand how belonging, power and privilege come to be inscribed within the governing nation-state.
NAViGaTing the Micronome: A Systematic Study of both the External Effects of MicroRNAs on Gene Repression networks, and the Contribution of microRNA Terminal Loops to MicroRNA Function
Keywords: MicroRNA
Computational Biology
Interaction Networks
Terminal Loops
mirDIP

The first aim of this thesis is to examine relationships between microRNAs targeting gene networks, combining knowledge from microRNA prediction databases into our microRNA Data Integration Portal (mirDIP). Modeling the microRNA:transcript interactome – referred to as the micronome – to build microRNA interaction networks of signalling pathways, we find genes within signalling pathways to be co-targeted by common microRNAs suggesting an unexpected level of transcriptional control. We identify two distinct classes of microRNAs; universe microRNAs, which are involved in many signalling pathways; and intra-pathway microRNAs, which target multiple genes within one signalling pathway. We find universe microRNAs to have more targets, to be more studied and more involved in cancer signalling than their intrapathway counterparts. The second aim was to undertake a more focused view, analyzing the characteristics of microRNAs within the micronome itself beginning with a focus on the under-examined microRNA terminal loop across the micronome to determine if this region of the microRNA structure might contribute to microRNA functioning. We have identified 2 main classes of microRNAs based on loop structure – perfect and occluded, which show biological relevance. We found regulatory motifs within microRNA terminal loops and found a large number of Frequently Occurring Words (FOWs) significantly overrepresented across the micronome. Set analysis of in vitro secreted microRNAs, microRNA expression across a panel of normal tissues, and microRNAs shown to be secreted in lung cancer shows that specific microRNA loop motifs within these groups are significantly overreperesented – suggesting that microRNA terminal loops harbour sequences bearing microRNA processing and localization signals.
Overlay Neighborhoods for Distributed Publish/Subscribe Systems
Keywords: Publish/Subscribe
Reliability
Fault-Tolerance
Middleware Systems
Multipath forwarding
Bulk content dissemination

The Publish/Subscribe (pub/sub) model has been widely applied in a variety of application scenarios which demand loose-coupling and asynchronous communication between a large number of information sources and sinks. In this model, clients are granted the flexibility to specify their interests at a high level and rely on the pub/sub middleware for delivery of their publications of interest. This increased flexibility and ease of use on the client side results in substantial complexity on part of the pub/sub middleware implementation. Furthermore, for several reasons including improved scalability, availability and avoiding a single point of failure, the pub/sub middleware is commonly composed of a set of collaborating message routers, a.k.a. brokers. The distributed nature of this design further introduces new challenges in ensuring end-to-end reliability as well as efficiency of operation. These challenges are largely unique to the pub/sub model and hence absent in both point-to-point or multicast protocols. This thesis develops solutions that ensure the dependable operation of the pub/sub system by exploiting the notion of overlay neighborhoods in a formal manner. More specifically, brokers maintain information about their neighbors within a configurable distance in the pub/sub overlay and exploit this knowledge to construct alternative forwarding paths or make smart forwarding decisions that improves efficiency, bandwidth utilization and delivery delay, all at the same time. Furthermore, in the face of failures overlay neighborhoods enable fast reconstruction of forwarding paths in the system without compromising its reliability and availability. Finally, as an added benefit of overlay neighborhoods, this thesis develops large-scale algorithms that bring the advantages of the pub/sub model to the domain of file sharing and bulk content dissemination applications. Experimental evaluation results with deployments as large as 1000 nodes illustrate that the pub/sub system scales well and outperforms the traditional BitTorrent protocol in terms of content dissemination delay.
Essays on International Trade, Productivity, and Growth
Keywords: productivity
heterogeneous firms
exports
complementarity
international trade
growth

This thesis investigates the role of institutions and firm behaviours in international trade. Chapter 1 estimates a dynamic general equilibrium model of entry, exit, and endogenous productivity growth. Productivity is endogenous both at the industry level (firms enter and exit) and at the firm level (firms invest in productivity-enhancing activities). Three key findings emerge. First, there is no evidence of learning by exporting: the observed positive correlation between exporting and productivity operates entirely via the impact of exporting on productivity-enhancing investments. Restated, exporting decision raises productivity, but only indirectly by making investing in productivity more attractive. Second, there is evidence of learning by producing multiple products: product-mix raises productivity directly in addition to the investment channel. Third, there are strong complementarities among the product-mix, exporting and investment decisions. Finally, we simulate the effects of reductions in foreign tariffs. This increases exporting, investing, and wages. Productivity rises at the economy-wide level both because of the between firm reallocation effect and because of within firm increases in productivity. Chapter 2 incorporates credit constraints into amodel of global sourcing and heterogeneous firms. Following Antras and Helpman(2004), heterogeneous firms decide whether to source inputs at arm’s length or within the boundary of the firm. Financing of fixed organizational costs requires borrowing with credit constraints and collateral based on tangible assets. The party that controls intermediate inputs is responsible for these financing costs. Sectors differ in their reliance on external finance and countries vary in their financial development. The model predicts that increased financial development increases the share of arm’s length transactions relative to integration in a country. The effect is most pronounced in sectors with a high reliance on external finance. Empirical examination of country-industry interaction effects confirms the predictions of the model. Chapter 3 examines whether financial development facilitates economic growth by estimating the effect of financial development on reducing the costs of external finance to firms. The data reveal substantial evidence of decreasing returns to the benefit of financial development in industries that are more dependent on external finance and countries with less financial frictions.
Coupling Temperature Sensing and Morphogenesis in the Pathogenic Fungus Candida albicans
Keywords: fungal pathogenesis
molecular chaperone

Temperature is a critical environmental signal, which exerts powerful control over the development and virulence of diverse microbial pathogens. Fungi, along with other microbial species, exploit a diversity of mechanisms to sense and respond to temperature fluctuations that may be encountered in the host or under other conditions of temperature stress. For Candida albicans, the leading fungal pathogen of humans, temperature influences mating, phenotypic switching, resistance to antifungal drugs, and the morphogenetic transition from yeast to filamentous growth. C. albicans morphogenesis is strongly influenced by temperature, and most filament inducing cues depend on a concurrent increase of temperature to 37˚C before morphogenesis can occur. Further elevated temperature of 39˚C to 42˚C can serve as an independent filament-inducing cue, although the molecular mechanisms underpinning this temperature-dependent morphogenetic transition remain largely uncharacterized. Here, I provide the first comprehensive investigation of the molecular mechanisms mediating temperature-dependent morphogenesis in C. albicans. I establish that the thermally responsive molecular chaperone Hsp90 orchestrates temperature-dependent morphogenesis, and that Hsp90 functions as a key temperature sensor, such that elevated temperature is required to relieve Hsp90-mediated repression of the morphogenetic program. Further, I demonstrate that Hsp90 controls morphogenesis via at least two distinct cellular signaling cascades. First, Hsp90 and its co-chaperone Sgt1 physically interact, and together regulate protein kinase A (PKA) signaling via an interaction with the adenylyl cyclase of the PKA cascade, Cyr1, such that genetic depletion of either Hsp90 or Sgt1 activates PKA signaling and induces filamentation. Second, Hsp90 controls temperature-dependent morphogenesis via previously uncharacterized cellular circuitry comprised of the cyclin-dependent kinase Pho85, the cyclin Pcl1, and the transcriptional regulator Hms1. Together, this research illuminates the central role of Hsp90 in coupling temperature sensing and morphogenesis in the human fungal pathogen C. albicans.
Electrochemical Studies of DNA Films on Gold Surfaces
Keywords: Electrochemical
Label free
Biosensor
DNA
Single nucleotide mismatch
Detection

DNA-metal ion interactions are critical for stabilizing conformations of double stranded (ds) DNA and through specific binding sites will influence the interaction of DNA with other molecules. It has been shown that different metal ions bind to different sites within nucleic acids. Work in this thesis exploits the interactions of Zn2+ with nucleic acids that are linked to surfaces. Zn2+ can interact with the phosphodiester backbone and engage in interactions with the purine nucleobases. Electrochemical studies of ds-DNA films have demonstrated that in the presence of Zn2+ films containing a single nucleotide mismatch give rise to a specific electrochemical signature. Electrochemical impedance spectroscopy (EIS) allows the discrimination of mismatched DNA films from those that are fully matched by monitoring differences in the resistance of charge transfer. Scanning electrochemical microscopy (SECM) allows multiplexing of the data acquisition and monitoring of the current response I, which is attenuated as a function of mismatch. In this thesis, various potential factors were explored in detail that may impact the discrimination of nucleotide mismatches in ds-DNA films by EIS and SECM. These factors include the position of the mismatch, its type, the number of mismatches, the length of the DNA duplex, and the length of target sequences. In particular, when the two strands are of unequal length, the resulting nucleotide overhang may mask the mismatch signature. Such overhangs are expected in real biosensor applications, in which the DNA is isolated from cellular targets. Results presented here clearly demonstrate that mismatches are readily distinguished from fully matched strands even in overhang systems, suggesting that this approach has promise for realistic sensor applications.
Novel Polysaccharide Based Polymers and Nanoparticles for Controlled Drug Delivery and Biomedical Imaging
Keywords: drug delivery, controlled release, nanoparticles, polysaccharides, theranostics

The use of polysaccharides as building blocks in the development of drugs and contrast agents delivery systems is rapidly growing. This can be attributed to the outstanding virtues of polysaccharides such as biocompatibility, biodegradability, upgradability, multiple reacting groups and low cost. The focus of this thesis was to develop and characterize novel starch based hydrogels and nanoparticles for delivery of drugs and imaging agents. To this end, two different systems were developed. The first system includes polymer and nanoparticles prepared by graft polymerization of polymethacrylic acid and polysorbate 80 onto starch. This starch based platform nanotechnology was developed using the design principles based on the pathophysiology of breast cancer, with applications in both medical imaging and breast cancer chemotherapy. The nanoparticles exhibited a high degree of doxorubicin loading as well as sustained pH dependent release of the drug. The drug loaded nanoparticles were significantly more effective against multidrug resistant human breast cancer cells compared to free doxorubicin. Systemic administration of the starch based nanoparticles co-loaded with doxorubicin and a near infrared fluorescent probe allowed for non-invasive real time monitoring of the nanoparticles biodistribution, tumor accumulation, and clearance. Systemic administration of the clinically relevant doses of the drug loaded particles to a mouse model of breast cancer significantly enhanced therapeutic efficacy while minimizing side effects compared to free doxorubicin. A novel, starch based magnetic resonance imaging (MRI) contrast agent with good in vitro and in vivo tolerability was formulated which exhibited superior signal enhancement in tumor and vasculature. The second system is a co-polymeric hydrogel of starch and xanthan gum with adjustable swelling and permeation properties. The hydrogels exhibited excellent film forming capability, and appeared to be particularly useful in controlled delivery applications of larger molecular size compounds. The starch based hydrogels, polymers and nanoparticles developed in this work have shown great potentials for controlled drug delivery and biomedical imaging applications.
Non-holomorphic Cuspidal Automorphic Forms of GSp(4;A) and the Hodge Structure of Siegel Threefolds
Keywords: Theta lifting
Siegel modular forms
Local theory

Let V( ) denote a local system of weight on X = A2;n(C), where X is the moduli space of principle polarized abelian varieties of genus 2 over C with xed n-level structure. The inner cohomology of X with coe cients in V( ), H3 ! (X;V( )), has a Hodge ltration of weight 3. Each term of this Hodge ltration can be presented as space of cuspidal automorphic representations of genus 2. We consider the purely non-holomorphic part of H3 ! (X;V( )) denoted by H3 Ends(X;V( )). First of all we show that there is a non-zero subspace of H3 Ends(X;V( )) denoted by V (K), where K is an open compact subgroup of GSp(4;A), such that elements of V (K) are obtained by the global theta lifting of cuspidal automorphic representations of GL(2) GL(2)=Gm. This means that there is a non-zero part of H3 Ends(X;V( )) which is endoscopic. Secondly, we consider the local theta correspondence and nd an explicit answer for the level of lifted cuspidal automorphic representations to GSp(4; F) over a non-archimedean local eld F. Therefore, we can present an explicit way for nding a basis for V (K) for a xed level structure K. ii There is a part of the Hodge structure that only contributes in H(3;0) ! (X;V( )) H(0;3) ! (X;V( )). This part is endoscopic and coming from the Yoshida lift from O(4). Finally, in the case X = A2, if eendo(A2;V( )) denotes the motive corresponded to the strict endoscopic part (the part that contributes only in non-holomorphic terms of the Hodge ltration), then we have eendo(A2;V( )) = 􀀀s 1+ 2+4S[ 1 􀀀 2 + 2]L 2+1; (1) where = ( 1; 2) and is far from walls. Here S[k] denotes the motive corresponded to Sk, the space of cuspidal automorphic forms of weight k and trivial level, and sk = dim(Sk). ii
Identifying Nursing Activities to Estimate the Risk of Cross-contamination
Keywords: Hospital acquired infection
Infection control
Hand hygiene
Accelerometer
Nursing activities
Sony PlayStation
Sixaxis
Transition points
Recursive least squares
Adaptive segmentation
Mathematical modelling
Markov Chain

Hospital Acquired Infections (HAI) are a global patient safety challenge, costly to treat, and affect hundreds of millions of patients annually worldwide. It has been shown that the majority of HAI are transferred to patients by caregivers' hands and therefore, can be prevented by proper hand hygiene (HH). However, many factors including cognitive load, cause caregivers to forget to cleanse their hands. Hand hygiene compliance among caregivers remains low around the world. In this thesis I showed that it is possible to build a wearable accelerometer-based HH reminder system to identify ongoing nursing activities with the patient, indicate the high-risk activities, and prompt the caregivers to clean their hands. Eight subjects participated in this study, each wearing five wireless accelerometer sensors on the wrist, upper arms and the back. A pattern recognition approach was used to classify six nursing activities offline. Time-domain features that included mean, standard deviation, energy, and correlation among accelerometer axes were found to be suitable features. On average, 1-Nearest Neighbour classifier was able to classify the activities with 84% accuracy. A novel algorithm was developed to adaptively segment the accelerometer signals to identify the start and stop time of each nursing activity. The overall accuracy of the algorithm for a total of 96 events performed by 8 subjects was approximately 87%. The accuracy was higher than 91% for 5 out of 8 subjects. The sequence of nursing activities was modelled by an 18-state Markov Chain. The model was evaluated by recently published data. The simulation results showed that the high-risk of cross-contamination decreases exponentially by frequency of HH and this happens more rapidly up to 50%-60% hand hygiene rate. It was also found that if the caregiver enters the room with high-risk of transferring infection to the current patient, given the assumptions in this study, only 55% HH is capable of reducing the risk of infection transfer to the lowest level. This may help to prevent the next patient from acquiring infection, preventing an infection outbreak. The model is also capable of simulating the effects of the imperfect HH on the risk of cross-contamination.
An Exploration of the Relationship between Poverty and Child Neglect in Canadian Child Welfare
Keywords: child neglect
child welfare
poverty
child maltreatment
child welfare policy

Objectives: Concerns have been raised that child welfare systems may inappropriately target poor families for intrusive interventions. The term “neglect” has been critiqued as a class-based label applied disproportionately to poor families. The objectives of the study are: to identify the nature and frequency of clinical and poverty-related concerns in child neglect investigations and to assess the service referral response to these needs; to examine the contribution of poverty-related need to case decision-making; and to explore whether substantiated cases of neglect can be divided into subtypes based on different constellations of clinical and poverty-related needs. Methods: This study is a secondary analysis of data collected through the 2008 Canadian Incidence Study of Reported Child Abuse and Neglect (CIS‑2008), a nationally representative dataset. A selected subsample of neglect investigations from the CIS‑2008 (N = 4,489) is examined through descriptive analyses, logistic regression, and two-step cluster analysis in order to explore each research objective. Results: Children and caregivers investigated for neglect presented with a range of clinical and poverty-related difficulties. Contrary to some previous research, the existence of poverty-related needs did not influence case dispositions after controlling for other relevant risk factors. However, some variables that should be, in theory, extraneous to case decision-making emerged as significant in the multivariate models, most notably Aboriginal status, with Aboriginal children having increased odds of substantiation, ongoing service provision and placement. Cluster analyses revealed that cases of neglect could be partitioned into three clusters, with no cluster emerging characterized by poverty alone. Conclusions: The majority of children investigated for neglect live in families experiencing poverty-related needs, and with caregivers struggling with clinical difficulties. While poverty-related need on its own does not explain the high proportion of poor families reported to the child welfare system, nor does it account for significant variance in case decision making, cluster analysis suggests that there exists a subgroup of “neglected” children living in families perhaps best characterized by the broader notion of social disadvantage. These families may be better served through an orientation of family support/family welfare rather than through the current residual child protection paradigm.