VictorJuiz/thesis huggingface dataset:
Alegal Midwives: Oral History Narratives of Ontario Pre-legislation Midwives
Keywords: midwifery
midwives
oral history
midwifery history
Ontario midwifery
North American midwifery
midwifery movements
alternative birth movements
midwifery revival
counterculture midwives
unregulated midwives
midwives' inspirations
midwives' motivations

This study examines the oral histories of midwives who practiced in Ontario without legal status in the two decades prior to the enactment of midwifery legislation on December 31, 1993. The following questions are answered: Who were Ontario’s pre-legislation midwives? What inspired and motivated them to take up practice on the margins of official health care? Current scholarship on late twentieth century Ontario midwifery focuses on a social scientific analysis of midwifery’s transition from a grassroots movement to a regulated profession. Pre-legislation midwives are commonly portrayed as a homogenous group of white, educated, middle class women practicing a “pure” midwifery unmediated by medicine and the law. Analysis of the oral history narratives of twenty-one “alegal” Ontario midwives reveals more complex and nuanced understandings of midwives and why they practiced during this period. The midwives’ oral histories make an important contribution to the growing historiography on modern Canadian midwifery.
Tritium Removal Facility High Tritium Distillation Simulation
Keywords: Modeling
Simulation
Thermodynamics
Nodalization
MATLAB
Simulink
Tritium
Distillation
Control
Cascade
PID
Tuning

A dynamic model was developed for the distillation mechanism of the Darlington Tritium Removal Facility. The model was created using the commercial software package MATLAB/Simulink. The goal was to use such a model to predict the system behaviour for use in control analysis. The distillation system was first divided into individual components including columns, condensers, controllers, heaters and the hydraulic network. Flow streams were then developed to transfer enthalpy, pressure and mass flow rate between the components. The model was able to perform various plant transients for validation and analysis purposes. A comparison of the different controllers was made with the introduction of various disturbances to the system. Also, the effect of the system disturbances when isolated from the transients was studied using the same controllers. Studying different plant transients and disturbances under each controller enabled a comparative analysis.
Comparison of CT and Optical Image-based Assessment of Liposome Distribution
Keywords: liposome
contrast agents
CT
optical
FMT

The use of multimodal imaging as a tool to assess the in vivo pharmacokinetics and biodistribution of nanoparticles is important in drug development and imaging-guided therapy. The current study reports the use of combined micro-CT and optical imaging FMT to quantitatively assess the whole body and intratumoural distribution of a nano-sized liposome-based CT/optical imaging probe carrying iohexol and Cy5.5. Micro-CT was used to guide the FMT tumour delineation and signal correction. This investigation demonstrates the critical role micro-CT can play in guiding FMT-based quantification of distribution. As well the combination of CT and optical imaging enable visualization of the liposomes at the whole body, tumor and cellular levels with high sensitivity and excellent anatomical background. Such non-invasive assessment of therapeutic distribution at the macro and micro scale is necessary for implementation of personalized medicine including image-guided patient stratification and real-time adjustment of therapeutic dose.
Methods for Data Analysis in Split-mouth Randomized Clinical Trials, a Simulation Study
Keywords: split-mouth trials
data analysis

Split-mouth trials are a design of randomized controlled trial in dentistry in which divisions of the mouth are the units of randomization. Since there is more than one tooth in each mouth division, the structure of the data is complex, which can create difficulties in the statistical analysis. The aim of this study was to determine what is the most appropriate method to analyze split-mouth trials with continuous outcomes, with regards to the treatment effect estimates, power, type-I error, confidence interval coverage and confidence interval width. A superiority split-mouth trial in the field of periodontology was simulated, using two mouth divisions and varying underlying study characteristics such as correlation among teeth, treatment effects and sample size. Twenty-four statistical methods were compared across 315 scenarios. The performance of the statistical methods depended mainly on the correlation among the data, and a paired t-test performed the best across the different scenarios.
Effects of Insulin Resistance on Leptin Modulation of Hypothalamic Neurons
Keywords: Neuroendocrinology
Hypothalamus
Obesity
Diabetes
Insulin resistance
Leptin
Insulin
Crosstalk
Hyperinsulinemia
Hypothalamic cell lines

Central resistance to the actions of insulin and leptin is strongly associated with obesity and type 2 diabetes mellitus (T2DM). These anorexigenic hormones modulate one another’s actions at the neuronal level. To investigate the cellular events underlying the effect of insulin resistance on leptin modulation of hypothalamic neurons, a neuronal cell model was established. The rHypoE-19 cell line expresses the insulin and leptin receptors alongside a complement of signaling molecules rendering it an appropriate model to study the molecular events underlying leptin and insulin crosstalk. Hyperinsulinemia was used to induce insulin resistance and leptin regulation of the rHypoE-19 neurons was analyzed prior to and following the induction of insulin resistance. It was found that the attenuation of insulin signal transduction affects leptin signaling and transcriptional modulation of the rHypoE-19 neurons. These studies will ultimately lend itself to an improved understanding of the complex cellular events that accompany neuronal hormone resistance.
Deep Brain Stimulation Suppresses Gamma Oscillations in Treatment Resistant Depression
Keywords: deep brain stimulation
major depressive disorder
treatment resistant depression
gamma oscillations
working memory
subcallosal cingulate gyrus

Background: Major depressive disorder is a debilitating psychiatric condition with high rates of treatment resistance that may be associated with working memory (WM) deficits. For treatment resistant depression (TRD) patients, deep brain stimulation (DBS) is emerging as an effective therapeutic option. Objective: To determine if electroencephalography signals recorded during DBS ON and OFF states while performing WM tasks can serve as biomarkers of therapeutic efficacy for DBS in TRD patients. Results: DBS stimulation suppressed frontal gamma oscillations (30–50Hz) during the ON state relative to the OFF state, an effect that was more pronounced with higher WM load. This suppression strongly correlated with depressive symptoms reduction. Conclusion: Suppression of gamma oscillations by DBS is likely mediated by indirect activation of inhibitory circuits in the frontal cortex. It represents a potential treatment biomarker for DBS in TRD and may lead to more tailored treatment parameters that can result in enhanced efficacy.
Phosgene-free Synthesis of Verdazyl Radicals and Enantioselective 1,3-dipolar Cycloaddition Reactions of Azomethine Imines Generated in situ from Verdazyl Radicals
Keywords: Verdazyl Radical
1,3-dipolar cycloaddition
enantioselective synthesis

Verdazyl radicals started receiving attention as substrates for organic synthesis only a few years ago. Since then, the chemistry of verdazyl radicals has advanced at a very fast rate. There are now a number of generations of novel molecular scaffolds derived from verdazyl radicals. Traditionally, verdazyl radicals have been synthesized from mono-substituted alkyl hydrazine and phosgene, which are extremely dangerous to handle. Alkyl hydrazines are restricted from being imported into certain countries, including Canada. A completely new alkyl hydrazine- and phosgene-free synthesis is reported in this thesis. The new synthesis, relative to previously reported syntheses of verdazyl radicals, is safer, more economical and provides the ability to derivatize verdazyl radicals to a larger extent. In addition, enantioselective 1,3-dipolar cycloaddition reactions with various metal- or organo-catalysts are reported. The project is still in progress with the highest e.e. of > 90%.
Interactive Flexible Switch Mode Power Supplies for Reducing Volume and Improving Efficiency
Keywords: Switch Mode Power Supply
Volume Reduction
Flexible Topology
Interactive Converter

The purpose of this thesis is to introduce a family of interactive Switch Mode Power Supplies (SMPS) for reducing the overall volume of the conventional converter topologies in low-to-medium power (up to 60W) applications. As shown in this thesis, the interaction between power supplies and electronic devices can be incorporated with emerging digital controllers for SMPS, to implement flexible converter topologies. These flexible topologies dynamically change the converter configuration, based on the load requirement, to provide near ideal transient response and/or improved efficiency over a wide range of operating conditions. This interaction relaxes the energy storage requirement for the converter reactive components and results in a low volume implementation. The interaction with the SMPS can be between the electronic load and the power supply or different conversion stages of a multi-stage converter. The effectiveness of the introduced family of SMPS is verified on digitally controlled dc-dc and ac-dc converter topologies.
Characterization of Structural and Binding Properties of 4E-BP2
Keywords: Translation initiation
Intrinsically disordered protein
Eukaryotic initiation factor 4E
4E-BP2
NMR
Fluorescence

Eukaryotic initiation factor-4E (eIF4E) controls the rate of cap-dependent translation initiation and is in turn exquisitely regulated by 4E-BPs. 4E-BP2 binds eIF4E with the highest affinity and is implicated in cancer, and metabolic and neurological disorders. Herein we use NMR, ITC and fluorescence to characterize 4E-BP2 structural and binding properties. Isolated 4E-BP2 is intrinsically disordered, but possesses some transient secondary structural propensities. eIF4E, however, is folded but has a disordered N-terminus. The eIF4E:4E-BP2 interaction is tight (Kd = 10-9 nM) and involves 4E-BP2 C-terminal and canonical binding regions, and the disordered eIF4E N-terminus. 4E-BP2 remains largely disordered upon binding to eIF4E. Noteworthy, high affinity interactions are not necessarily mediated by static structures, and 4E-BP2 binding is not the simple “disorder-to-order” transition observed in many interactions involving disordered proteins. This study offers molecular insights into 4E-BP2 functionality, and lays a foundation for development of novel therapies for cancer and neurological disorders.
Dietary Implications of Interactions between Ants and Symbiotic Bacteria
Keywords: Ants
Bacteria
Symbiosis

Studies of symbiotic bacteria have demonstrated that they provide multiple benefits to their hosts. These studies, however, have overlooked the importance of interactions with other bacteria and environmental factors that affect bacterial assemblages. To understand what shapes bacterial assemblages, I manipulated the diet of ants from the genus Cephalotes and disturbed their gut microbiome. I found that a deficit of nitrogen reduces bacterial densities. Furthermore, the data suggest that bacterial abundance may influence ant survival. I followed this experiment up by manipulating a putative protein source in the field. Our lab assigned Allomerus octoarticulatus ant colonies to treatments in which potential prey were present or absent. I collected data on foraging behaviour, colony performance, and composition of the bacterial community. The absence of prey increased ant recruitment to protein-rich baits; these ants were also less fit than ants that had insect prey but their bacterial assemblages were not affected.
Auger Electron-emitting Radioimmunotherapeutic (RIT) Agent Specific for Leukemic Stem Cells
Keywords: auger electron radioimmunotherapy
acute myeloid leukemia
leukemic stem cells
CD123
111-indium

Objective: CSL360 is a chimeric IgG1 mAb recognizing CD123+/CD131- LSCs responsible for acute myeloid leukemia (AML). The in vitro targeting properties of 111In-labeled CSL360 modified with nuclear localization sequence (NLS) were evaluated in AML cells. Methods: 111In-NLS-CSL360 was constructed and its binding affinity, cellular uptake and nuclear importation were analyzed on CD123+ cells. Cytotoxicity was evaluated by clonogenic assays on AML cells (CD123+/CD131-). Results: 111In-NLS-CSL360 exhibited preserved binding to CD123. High cellular and nuclear uptake was observed at 266 nM after 24 hour of incubation. Nuclear uptake of 111In-NLS-CSL360 (266 nM) was 2.0-fold higher than 111In-CSL360 (266 nM) after 24 hour of incubation. Clonogenic survival (CS) of AML cells was reduced to 27.5 ± 4.1%. The nuclear uptake and cytotoxicity were reduced when pre-exposed to unlabeled CSL360, indicating 111In-NLS-CSL360 was CD123-specific. Conclusion: 111In-NLS-CSL360 could be a promising radioimmunotherapeutic agent specific for LSCs.
Localized Corrosion of FrictionStir Spot Welds in AZ31 Magnesium Alloys
Keywords: Corrosion
Magnesium
Friction Stir Spot Weld
AZ31

A scanning reference electrode technique (SRET) apparatus has been designed and commissioned to investigate the corrosion of friction stir spot welds (FSSW) made in AZ31 magnesium alloys. The operational parameters of the apparatus have been calibrated to give good spatial resolution. By combining the SRET data with material flow data and immersion test data it was found that the FSSW process caused the formation of distinct noble and active regimes within the weld area. The noble region was aligned with the stir zone (SZ) and was caused by a dynamically recrystallized grain structure which is void of dislocations / twins, and β Mg17Al12. Localized corrosion attack was observed in both SRET and immersion testing along the thermo-mechanically affected zone (TMAZ). The same effect was consistently observed with a flat versus concave shoulder tool, and dwell times of 1s and 4s.
Highly Transparent Glass using Nanoparticle Films for Enhanced Optoelectronics
Keywords: antireflection coats
glass

This thesis provides an investigation and review of homogeneous multilayer anti-reflective coatings (ARC) on glass. Recently, numerical optimization has become popular in optimizing the number of layers at a defined wavelength range and at angular incident angles. In this investigation, the design of the index profile is optimized for normal incident angle and angular incident angles by an evolutionary genetic algorithm. The anti-reflective coatings consist of multilayer porous silica or tin oxide nanoparticle films, which are fabricated by mixing 10nm silica nanoparticle or 10nm tin oxide nanoparticle colloidal solutions with varying amounts of 50nm polystyrene colloidal solutions, followed by spin coating on a glass substrate, and sintering at 400˚C for 40 minutes, which burns off the embedded polystyrene and renders a voided matrix. Experiments were carried out to produce ARCs based on well-known index profiles, and based on genetic algorithm optimization
Glycemic Index, Oxidized LDL, and CHD Risk
Keywords: Glycemic Index
CHD
Oxidative Damage
Heart Disease

The aim was to determine whether the dietary glycemic index (GI) related to coronary heart disease (CHD) risk and whether oxidized LDL could explain this relation. Nine prospective cohorts of GI or glycemic load (GL) associations were pooled in a meta-analysis and showed an increased risk of CHD for high GI (near significant at RR=1.13, 95%CI; 1.00-1.26) and GL diets (significant at RR=1.40, 95%CI; 1.17-1.68), both with significant evidence of heterogeneity (P<0.07). Sera from 151 type 2 diabetics who completed a 6-month trial of a low GI diet demonstrated no treatment difference in measures of oxidative damage. However, when data from both treatments were pooled, oxidized LDL as a marker of CHD risk inversely related to low GI carbohydrate intake. We conclude that GI and GL relate to CHD and oxidative damage to LDL may explain part of this association.
Characterization of Escherichia coli Single-gene Deletion Mutants Impaired in Bacteriophage Reproduction
Keywords: Bacteriophage
Bacteriophage Resistance
Genetic Screen
Escherichia coli

An assay was designed to measure the sensitivity of Escherichia coli mutants to bacteriophage infection via growth curves, using a Tecan temperature controlled plate reader. I screened 3985 single-gene deletion strains in Escherichia coli K12 from the Keio collection and identified 43 strains displaying varying degrees of resistance to four different phages, three non-contractile tailed phages (λcI857, HK97, and HK243) and the myoviridae T6, including 20 genes not previously implicated in phage infection. Additional assays, such as adsorption and tests of DNA-injection, were designed to further characterize resistant strains. The use of these assays helped identify varying sensitivities to LPS structure and LamB receptor concentration in the three non-contractile tailed phages, showing HK97 is the most sensitive to changes and HK243 the least. I also found that the periplasmic chaperone, FkpA, is required for HK97 DNA-injection.
Barriers & Facilitators to Overcoming Obesity in Canada and the Role of Fat-related Nutrient Content Claims
Keywords: obesity
nutrient content claims
nutrition policy
nutrition regulations
fat claims
weight management
food choice
consumer survey
nutrition labelling

Our overall objective was to explore some individual and environmental determinants of eating habits with a main focus on nutrition labels and their usefulness for choosing foods for weight management. We first broadly examined these determinants using participants of the AFMNet Canadian Consumer Monitor (CCM), and found a number of attitudinal and perceived environmental barriers to healthy eating. In an experimental study with CCM respondents, we found that reduced fat claims may help consumers make better choices for weight management if calorie content on the Nutrition Facts Table is also considered. However, in our examination of foods with fat claims in the Canadian marketplace, we found that most products with fat claims are not significantly lower in calories than comparable foods without such claims. We conclude that fat claims could be useful in assisting consumers with food choices for weight management, if the foods were also reduced in calories.
Molecular Characterization of a Recurrent t(2;7) Translocation Linking CDK6 to the IGK Locus in Chronic B-cell Neoplasia
Keywords: Genetics
Malignancy
Hematology
B-cell Neoplasia
Chromosomal Translocation
Monoclonal B-cell Lymphocytosis
Chronic Lymphocytic Leukemia
Non-Hodkin Lymphomas
PCR
CDK6
IGK

Uncovering the chromosomal abnormalities associated with human malignancy can provide significant insights into the molecular basis of tumorigenesis, as well as identifying potential targets for therapy. The present study set out to examine the genetic characteristics of t(2;7)(p11-12;q21-22) translocations arising in conjunction with chronic B-cell neoplasia. Using long-range PCR, a t(2;7) was initially mapped in an individual presenting with the preclinical entity CD5- monoclonal B-cell lymphocytosis. This revealed a breakpoint at 2p11.2 localized to the recombination signal of the immunoglobulin kappa (IGK) variable gene IGKV3-15, and a breakpoint at 7q21.2 located 520 bp upstream of cyclin dependent kinase 6 (CDK6). The same approach was subsequently employed to elucidate near-identical t(2;7) breakpoints in 4 additional cases presenting with chronic lymphocytic leukemia or indolent non-Hodgkin lymphomas. The remarkable consistency of these translocations implicates the dysregulation of CDK6 via translocation to IGK as a recurrent pathomechanism during the emergence of B-cell lymphoproliferative disorders.
Hybrid Organic / Inorganic Solar Cells Based On Electrodeposited ZnO Nanowire Arrays on ITO and AZO Cathodes
Keywords: ZnO Nanowire Arrays
Hybrid Organic / Inorganic Solar Cells

ZnO nanowire arrays (NWAs) and Al-doped ZnO (AZO) cathodes were applied in hybrid organic / inorganic solar cells for lower-cost solar energy. Parameters for the electrodeposition of ZnO NWAs and the fabrication of NWA-free baseline devices were systematically optimized using ITO as the cathodes. High efficiencies of up to 5.4% were achieved. Incorporation of the ZnO NWAs into the baseline devices significantly reduced their efficiencies due to possible shorting in the active layer. Devices fabricated using AZO cathodes were characterized. The AZO-based devices achieved efficiencies of up to ~4.8%, showing promising results for the application of AZO as an ITO alternative. Formation of numerous large nanoplatelets was observed during the electrodeposition of ZnO NWAs on AZO cathodes. The NWAs grown on AZO cathodes were also non-uniform. Future studies were proposed to address the issues with incorporation of ZnO NWAs in hybrid solar cells and their combination with AZO cathodes.
The Role of Adult Neurogenesis in Contextual Learning and Memory Interference
Keywords: Behaviour
Neurogenesis
Interference
Irradiation

New neurons are continually produced throughout adult life in the dentate gyrus of the hippocampus, in a process termed adult neurogenesis. Although there is a significant effort in the literature to understand the functional significance of hippocampal neurogenesis, conflicting experimental reports have left the role of neurogenesis unclear. Recently, computational modelling studies have hypothesized that neurogenesis may play a role in allowing association between event and context to be formed in memory. By using a novel odour task and a raised plus maze task, our work demonstrates that the reduction of hippocampal neurogenesis using focal irradiation impairs the ability of animal subjects to utilize contextual information to learn interfering information. The result of this work provides experimental evidence of a unique role neurogenesis may play in learning and memory.
Predictive Factors for Outcome in Patients having Surgery for Cervical Spondylotic Myelopathy.
Keywords: cervical spondylotic myelopathy
magnetic resonance imaging
predictors
surgical outcomes

PURPOSE: The objective was to determine if particular magnetic resonance, clinical and demographic findings were associated with functional status prior to surgery and predictive of functional outcomes at follow-up. RESULTS: The study included 65 consecutive CSM patients. The modified Japanese Orthopaedic Association Scale (mJOA) was used as the primary outcome measure. Higher baseline mJOA scores were associated with younger age, shorter duration of symptoms, fewer compressed segments and less severe cord compression. Better post-operative mJOA scores were associated with younger age, shorter duration of symptoms and higher baseline scores. Using multivariate analysis, baseline and follow-up mJOA scores adjusted for baseline mjOA score were best predicted by age. CONCLUSION: Age and clinical severity scores at admission can both provide valuable information. However, MR imaging features of the spinal cord before surgery cannot accurately predict the functional prognosis for patients with CSM and hence alternative imaging approaches may be required.