Public procurement notice: Contract Notice
Published: 2023-02-27
Buyer: Ss. Cyril and Methodius University Faculty of Medicine Skopje
Country: MK
Contract type: Supplies
Procedure: Open procedure
CPV classification: 33696500
Submission deadline: 2023-03-24
Award criteria: Lowest price

Technical requirements: A set of reagents for the extraction of DNA from liquid samples (blood and saliva), blood on FTA cards or textile material, body fluids (saliva, semen) on textile material, body fluids on swabs, valid
A set of reagents for the extraction of DNA from liquid samples (blood and saliva), blood on FTA cards or textile material, body fluids (saliva, semen) on textile material, body fluids on swabs, validated for forensic analysis, to include at least the following reagents : a minimum of 27 ml of lysing solution, at least 52 cartridges, at least 52 sample collection tubes, at least 52 elution tubes, at least 52 columns, at least 52 extensions and extension holders, compatible with an Automate Express instrument or equivalent, validated for forensic analysis
A next-generation diagnostic genetic sequencing kit for the quantification of donor-derived free-circulating DNA (cfDNA) in post-transplant patients. The kit should be based on a multiplex PCR method
A next-generation diagnostic genetic sequencing kit for the quantification of donor-derived free-circulating DNA (cfDNA) in post-transplant patients. The kit should be based on a multiplex PCR method for the preparation of sequencing libraries of a plasma-derived cfDNA extract with PCR primers covering a minimum of 202 single nucleotide polymorphisms. Be compatible with MiniSeq or equivalent. Contain software that provides a value for the presence of cfDNA. The kit must include the following reagents: PCR mix, PCR enzyme, SNP Primer Pool, Index Primers (Forward i5 and Reverese i7) for min 24 tests, 2N NaOH, Purification beads and buffer for resuspension.
Group: 365. KITS AND CONSUMABLES COMPATIBLE ON THE ILLUMINA DEVICE OR EQUIVALENT (The lot is not divisible)
Group: 368. Kits and reagents for next-generation sequencing (NGS) on the principle of sequencing by synthesis with the assignment of the necessary equipment to use (The lot is not divisible)
Whole human exome sequencing, including DNA sample delivery costs, targeted exome size ≥ 36.5 Mb, coverage of regions in Consensus Coding sequence-CCDS20≥99.9 %, RefSeq ≥ 99.2 % and GeneCode V28 ≥ 99,
Whole human exome sequencing, including DNA sample delivery costs, targeted exome size ≥ 36.5 Mb, coverage of regions in Consensus Coding sequence-CCDS20≥99.9 %, RefSeq ≥ 99.2 % and GeneCode V28 ≥ 99, 8 %, mitochondrial DNA coverage = 100 % (16569 bp/ 37 genes), sequencing output size ≥ 12 Gb, number of aligned reads in targeted regions ≥ 70%, number of sequenced bases with >30x coverage ≥ 95% , number of sequenced bases with Q30 quality ≥ 85 %). For bioinformatic data processing, comparison should be made to GRCh37/hg19 or a newer reference genome. Additional data filtering (gene panels) to be possible if necessary for certain cases. The estimated time for the analysis should be ≤ 20 working days. Final sequencing data should be in the following file formats (.fastq, .bam, .vcf, .tsv (SNP), .tsv (CNV)). The transfer of the final data should be done via sFTP or in special cases via HDD.
Analysis of whole genome sequencing data to contain gene and variant information from relevant sources including but not limited to CIViC, ClinVar, Clinical Genome Database (CGD), Clinical Trials, COS
Analysis of whole genome sequencing data to contain gene and variant information from relevant sources including but not limited to CIViC, ClinVar, Clinical Genome Database (CGD), Clinical Trials, COSMIC, dbSNP, Ensembl, InterPro , OMIM (via Ensembl), Orphanet, PFAM, Reactome , RefSeq. To support analysis of SNVs, Indels, CNVs and insertions in both germline and somatic contexts. To support at least csv/tsv, gff/gtf, vcf files. To indicate variants with the possibility of assessing their quality using a quality control system. To enable automated classification of variants according to ACMG, with the possibility of changing the classification by the user. Be able to create new and edit existing filtering strategies. To allow samples to be reopened for reanalysis. To enable the organization of the samples in groups with the aim of group-organized analysis of the data. Access to the cloud for at least two people.
Group: 370. Tests, kits and reagents for forensic panels for precise identification compatible on S5/Ion Chef platform or equivalent (Lot is not divisible)
Group: 479. Tests, kits and reagents for next generation preparation and sequencing, compatible on S5/Ion Chef platform or equivalent (Lot is not divisible)

Eligibility requirements: — statement by the economic operator that it has not been sentenced, in the last 5 years, with effective court decision for participation in criminal organisation, corruption, fraud or money laundering, — certificate that it is not subject to bankruptcy procedure issued by competent authority, — certificate that it is not subject to liquidation procedure issued by competent authority, — certificate for paid taxes, contributions and other public dues by the competent authority in the country where that economic operator is established, — certificate from the legal entities penal registry that it is not being imposed a side sanction — prohibition to participate in public competitions, public procurement contract award and public — private partnership contract award procedures, — certificate from the legal entities penal registry that it is not being imposed a side sanction — temporary or permanent prohibition to perform certain professional activity, — certificate that it is not being sanctioned for misdemeanor with an effective court decision, resulting in prohibition for pursuing professional activity or duty, i.e. temporary prohibition for performing certain business activity. Ability of the economic operator to perform business activity: — document for registered business activity - Certificate for wholesale of medical devices in accordance with the Law on Drugs and Medical Devices