Public procurement notice: Voluntary Ex Ante Transparency Notice
Published: 2019-09-13
Buyer: Oslo Universitetssykehus HF (hovedenhet)
Country: NO
Contract type: Supplies
Procedure: Contract award without prior publication
CPV classification: 33100000
Estimated value: 36060403 NOK
Award criteria: The most economic tender

Technical requirements: Avdeling for medisinsk genetikk, OUS, [the Department of Medical Genetics at Oslo University Hospital (OUS)] will procure sequencers for high throughput sequencing (HTS)/next generation sequencing, well suited to large scale exome and genomic sequencing. The department has HiSeq4000 and HiSeq3000 that can be used as back-up during downtimes.
The laboratory needs both exome and genomic sequencing for various issues. To maintain a quick response time, it is important that these can be run on the same flow cell. The department has a need for very large capacity, as samples for many different purposed must be sequenced and analysed. It is important that he response time and analysis time are kept to a minimum. The requirement highlights the need for flexibility in regard to whether analysis time or capacity should be prioritised, as there will be instances where urgent samples must be processed. These will have an acceptable analysis time of 30 hours. The instrument must be able to handle different protocols in order to cover the different purposed that the samples are sequenced for. PCR free sample preparation will be quicker (with multiple benefits), while other protocols needed can only be found PCR based. It is very important that the sample preparation is automatic and fast. There are multiple reasons for this: Less labour time is required, less danger for pipetting error and sample mix-up, and also standardised sample flow and protocol. The Department has Hamilton MicroStar pipetting robots for this purpose, and therefore there must be finished validated sample preparation protocols for Hamilton MicroStar for exome and genomic sequencing. Preferably, other protocols must also be adapted to Hamilton MicroStar. Different protocols can be optimal with different reading lengths. For a large number of analyses, longer fragments such as 250 bp will be an advantage, as this for e.g. can lead to seeing longer haplotypes, have higher sensitivity in regard to structural variation, and be advantageous to be able to separate pseudogenes from real genes. The Department also offers 16S metagenomic sequencing as a service, and for this utilises a procedure with 250 bp reads (https://microbiomejournal.biomedcentral.com/articles/10.1186/s40168-017-0279-1). The instrument must be flexible to also have protocols for shorter reading lengths, as this will be of benefit in regard to costs,and that for some protocols it will not be necessary to have 250 bp reading lengths. FASTQ, BAM and VCF are standard formats for the software programmes that the Department utilises for the analysis and interpretation of sequencing data. There is a need for < 48 hours response time from when OUS contacts the service provider until the service technician is in place. This is very important to maintain delivery deadlines, and thus downtime must be limited. It is believed that due to technical reasons, Illumina is the only service provider that can deliver in accordance with the requirements. The intention notice is pursuant to the public procurement regulations § 21-5. The terms in PPR § 13-4 (2) and 13-1 (5) are considered fulfilled, as well as the value of the full service contract and a contract for consumables for the lifetime of the equipment (the equipment lifetime is estimated to be 5 years).
The laboratory needs both exome and genomic sequencing for various issues. To maintain a quick response time, it is important that these can be run on the same flow cell. The department has a need for very large capacity, as samples for many different purposed must be sequenced and analysed. It is important that he response time and analysis time are kept to a minimum. The requirement highlights the need for flexibility in regard to whether analysis time or capacity should be prioritised, as there will be instances where urgent samples must be processed. These will have an acceptable analysis time of 30 hours. The instrument must be able to handle different protocols in order to cover the different purposed that the samples are sequenced for. PCR free sample preparation will be quicker (with multiple benefits), while other protocols needed can only be found PCR based. It is very important that the sample preparation is automatic and fast. There are multiple reasons for this: Less labour time is required, less danger for pipetting error and sample mix-up, and also standardised sample flow and protocol. The Department has Hamilton MicroStar pipetting robots for this purpose, and therefore there must be finished validated sample preparation protocols for Hamilton MicroStar for exome and genomic sequencing. Preferably, other protocols must also be adapted to Hamilton MicroStar. Different protocols can be optimal with different reading lengths. For a large number of analyses, longer fragments such as 250 bp will be an advantage, as this for e.g. can lead to seeing longer haplotypes, have higher sensitivity in regard to structural variation, and be advantageous to be able to separate pseudogenes from real genes. The Department also offers 16S metagenomic sequencing as a service, and for this utilises a procedure with 250 bp reads (https://microbiomejournal.biomedcentral.com/articles/10.1186/s40168-017-0279-1). The instrument must be flexible to also have protocols for shorter reading lengths, as this will be of benefit in regard to costs,and that for some protocols it will not be necessary to have 250 bp reading lengths. FASTQ, BAM and VCF are standard formats for the software programmes that the Department utilises for the analysis and interpretation of sequencing data. There is a need for < 48 hours response time from when OUS contacts the service provider until the service technician is in place. This is very important to maintain delivery deadlines, and thus downtime must be limited. It is believed that due to technical reasons, Illumina is the only service provider that can deliver in accordance with the requirements. The intention notice is pursuant to the public procurement regulations § 21-5. The terms in PPR § 13-4 (2) and 13-1 (5) are considered fulfilled, as well as the value of the full service contract and a contract for consumables for the lifetime of the equipment.


Winner: Illumina Norway AS
Award value: 36060403