Public procurement notice: Contract Award Notice
Published: 2022-03-04
Buyer: Aarhus Universitet
Country: DK
Contract type: Supplies
Procedure: Negotiated without a prior call for competition
CPV classification: 33000000 | 38000000
Estimated value: 1023000 DKK
Award criteria: Lowest price

Technical requirements: Purchase of a capillary electrophoresis (CE) system for analysis of protein-surfactant interactions. This will allow us to monitor multiple populations of co-existing protein-surfactant species and provide further insight into different mechanisms of protein denaturation by surfactants.
The Sciex P/ACE MDQ automated CE is the only CE apparatus available on the market that combines precise temperature control of the capillary and stored samples, pressurizes the capillary from either or both sides, includes 96-well plates, allows automatic tests of different sample-buffer combinations for method development, provides fraction collection, combines CE on protein-sodium dodecyl sulfate (SDS) with capillary isoelectric focusing (cIEF) and capillary zone electrophoreses (CZE) in one unit and can be upgraded to include laser-induced fluorescence (LIF) detection.
The UNISURF project will develop a universal model for unfolding of proteins in different surfactants (both anionic and cationic) based on a comprehensive analysis of the structures, populations and energetics of protein-surfactant complexes formed at a range of surfactant concentrations. We therefore need to be able to monitor the formation of different protein-surfactant species under a wide range of different conditions, including variations in buffer composition, temperature and protein-surfactant ratios. As the complexes are very temperature sensitive, sll measurements have to be done on accurate temperature control. As the structure of complexes has to be determined by small-angle X-ray scattering (SAXS), collection of different fractions of the separated species is crucial.
An automated CE setup with accurate temperature control and fraction collection for being able to monitor multiple populations of co-existing protein-surfactant species so that we can obtain further insight into different mechanisms of protein denaturation by surfactants, and use collected fractions for further SAXS experiments. For higher sensitivity, we need to be able to add LIF detection, should it turn out to be required.
The Sciex P/ACE MDQ automated CE is the only CE apparatus available on the market that combines precise temperature control of the capillary and stored samples, pressurizes the capillary from either or both sides, includes 96-well plates, automatically tests different sample-buffer combinations for method development, provides fraction collection, combines CE-SDS with cIEF and CZE in one unit and can be upgraded to laser-induced fluorescence.
There has been a systematic search for commercially available CE system offering accurate temperature control, automated sample mixing and handling, fraction collection, multiple applications in one unit and possibility for upgrade to laser-induced fluorescence detection.
The Sciex P/ACE MDQ automated CE is the only CE apparatus available on the market that fulfills these needs based on a perusal of available models.
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Winner: Ramcon
Award value: 1023000
Number of bids: 1