Public procurement notice: Voluntary Ex Ante Transparency Notice
Published: 2020-01-20
Buyer: Helmholtz Zentrum München GmbH
Country: DE
Contract type: Supplies
Procedure: Contract award without prior publication
CPV classification: 38000000
Estimated value: 1 EUR
Award criteria: Lowest price

Technical requirements: Installation, acceptance, initial start-up of the microscope at HMGU NeuherbergVisoScope Spinning DiskConfocal system
— the system must include a hardware-only based, optical device that forms a uniform flatfield illumination.Adaptable to achieve optimal illumination on a specific objective (e.g 100X),
— the sensitivity requires the microscope to lay on a vibration free microscopy table: size 900 x 1200 mm, 59 mm thick table plat, refined steel surface, max. weight 240 kg, M6 drill hole, 25 mm distance,carrier for arm pad, with pneumatic vibration damper,
— the Scanning head must enable simultaneous dual-color Fluorescence Imaging and include dual camera usage
— the Scanning head must have an automatic dichroic-mirror changer 3-position for dual camera mode, dual disc technology, standard disk with 50 um pinhole, effective field of view 17 x 16 mm, reduced light crosstalk because of larger distance of pin-hole, spinning speed 1 500 – 4 000 rpm, automatic dichroic changer 3-position, motorized emissions filter wheel 10-position, motorized Brightfield Light Path, connector for 2 digital CCD cameras. Variable Aperture for CCD Chip matching, motorized lens switcher C-mount, with additional 2,0 x lens,
— the CSU-W1 filter set must include a multiband filterset for 405/488/561/640 nm with single emission filter (Dapi ET460/50, GFP ET525/50, mCherry 609/54).
— to continuously provide optimal and fully aligned lasers for full laser power at the objective and thus, critically improve image acquisition of larvae, a motorized laser merge module with automatic fine-tuning of laser power is required,
— have the possibility to be upgraded to up to 6 lasers, required laser modules are: laser 405 nm with 150 mW, Laser 488 nm with 150 mW, Laser 561 nm with 150 mW, All laser with ‘fast Trigger TTL’ mode and individual control over the intensity of each of the selected lines,
— the device must include: a 3 channel laser merge module, which combines the beams of 3 lasers to a single colinear laser beam, usage of dichroics offering low intensity loss, an adapter kit to mount Lasers on baseplate for stable configuration of all components, micro lens FC coupling for fiber guide single mode and a system for selection of Laser Wavelength.
— inverted microscope with motorized lightpath (100%L; 100 % binocular; 100 % L; 80%L/20% ocular); magnification changer 1,5x. Motorized, encoded Z-focusing unit (speed 2,5 mm/s, minimum increment 10 nm) with 2 speed levels. Motorized system condenser with 7 positions for brightfield, phase contrast, DIC and NAMC contrast. DIC module: DIC standard polarizer, DIC slide 40x I, DIC slide 100x II, fixed central axis, mot.
Filter block turret 6 positions for filter; motorized shutter. P-apo 10x lambda/0,45/4,0; P-fluoro 40X oil/1,30/0,20; P-Apo 100x lambda oil/1,45/0,13.
— EM-CCD camera with the following characteristics: 1024 x 1024 pixel, 13 x 13 μm, frame transfer CCD, about 90 % QE, 16 BIT ADC, 30 and 10 Mhz operation, 26 full-frame/sec, thermo-electrically cooling down to -80
— white light LED Illumination system is required with the following features: fluorescence light source for microscopy, ‘white-light’ fluorescence excitation, wide spectral range: 360-680 nm, TTL trigger for on/off, with remote control pad, direct microscope adaption.
— image acquisition Software for PC with: realtime data acquisition, realtime display and screen operation, time-lapse function and fast streaming, player for movie sequences, support for all standard file formats, real-time overlay multichannel fluorescence combine, a complete microscope control incl. 5D visualization, scanning of Multiwell (for large scale worm screens), scanslide, realtime processing, an IronPython based macro language with auto-complete function; macros assignable to Toolbars,
— a precise TTL synchronization and blanking during sensor shift of EM-CCD cameras. To optimize image acquisition, a module to allow communication of the software functions from third-party software or client,


Winner: Visitron Systems GmbH, Puchheim
Award value: 1