Study: NCT05535738

Intervention type: DRUG
Group type: EXPERIMENTAL
Intervention: Squaric Acid Dibutyl Ester
Description: Sensitization dose: 2% Elicitation doses: {0.0001%, 0.00025%, 0.00075%, 0.001%, 0.0025%, 0.005%, 0.0075%, 0.01%, 0.025%, 0.05%, 0.06%, 0.07%, 0.08%, 0.09%, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6% 0.7%, 0.8%, 0.9%, 1.0%, 1.2%, 1.4%, 1.6%, 1.8%, 2.0%}

Intervention type: DRUG
Group type: EXPERIMENTAL
Intervention: Squaric Acid Dibutyl Ester
Description: Sensitization dose: 2% Elicitation doses: {0.0001%, 0.00025%, 0.00075%, 0.001%, 0.0025%, 0.005%, 0.0075%, 0.01%, 0.025%, 0.05%, 0.06%, 0.07%, 0.08%, 0.09%, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6% 0.7%, 0.8%, 0.9%, 1.0%, 1.2%, 1.4%, 1.6%, 1.8%, 2.0%}

Intervention type: OTHER
Group type: EXPERIMENTAL
Intervention: Known patch test allergens
Description: Positive patch test allergens during the course of clinical patch testing will be re-applied on the back followed by skin sampling

Intervention type: OTHER
Group type: EXPERIMENTAL
Intervention: Known patch test allergens
Description: Positive patch test allergens during the course of clinical patch testing will be re-applied on the back followed by skin sampling

Intervention type: DRUG
Group type: EXPERIMENTAL
Intervention: Dupilumab
Description: 300mg

Intervention type: DRUG
Group type: EXPERIMENTAL
Intervention: Adalimumab
Description: 40mg

Intervention type: DRUG
Group type: EXPERIMENTAL
Intervention: Ustekinumab
Description: 45mg

Intervention type: DRUG
Group type: EXPERIMENTAL
Intervention: Guselkumab
Description: 100mg

Intervention type: DRUG
Group type: EXPERIMENTAL
Intervention: Canakinumab
Description: 150mg

Intervention type: DRUG
Group type: EXPERIMENTAL
Intervention: Sarilumab
Description: 200mg

Intervention type: DRUG
Group type: EXPERIMENTAL
Intervention: Triamcinolone Acetonide
Description: 0.1% ointment

Intervention type: DRUG
Group type: EXPERIMENTAL
Intervention: Betamethasone Valerate
Description: 0.1% ointment

Intervention type: DRUG
Group type: EXPERIMENTAL
Intervention: Fluticasone Propionate
Description: 0.005% ointment

Intervention type: DEVICE
Group type: EXPERIMENTAL
Intervention: Microneedle
Description: Painless and non-scarring skin sampling with a 7mm x 7mm patch of hydrogel-coated poly-l-lactide microneedles (\<2mm length) will be used to collect interstitial fluid

Intervention type: DEVICE
Group type: EXPERIMENTAL
Intervention: Microneedle
Description: Painless and non-scarring skin sampling with a 7mm x 7mm patch of hydrogel-coated poly-l-lactide microneedles (\<2mm length) will be used to collect interstitial fluid

Intervention type: DEVICE
Group type: EXPERIMENTAL
Intervention: Suction blistering
Description: Suction blistering is a technique to induce and collect blister fluid using a negative pressure instrument (Electronic Diversities Finksburg, MD). It does not require local anesthetic, stitches or pain medication following the procedure. The blisters will be no greater than 1cm in diameter and no deeper than the epidermis (\<1mm deep). This process of inducing blisters is typically less than 1 hour. After the formation of blisters, the blister fluid will be extracted using a syringe. The blister roofs will be left attached to the skin and covered with petrolatum and a bandage.

Intervention type: DEVICE
Group type: EXPERIMENTAL
Intervention: Suction blistering
Description: Suction blistering is a technique to induce and collect blister fluid using a negative pressure instrument (Electronic Diversities Finksburg, MD). It does not require local anesthetic, stitches or pain medication following the procedure. The blisters will be no greater than 1cm in diameter and no deeper than the epidermis (\<1mm deep). This process of inducing blisters is typically less than 1 hour. After the formation of blisters, the blister fluid will be extracted using a syringe. The blister roofs will be left attached to the skin and covered with petrolatum and a bandage.

Intervention type: PROCEDURE
Group type: EXPERIMENTAL
Intervention: Skin punch biopsy
Description: A skin biopsy is the removal of a small piece of tissue, under local anesthetic.

Intervention type: PROCEDURE
Group type: EXPERIMENTAL
Intervention: Skin punch biopsy
Description: A skin biopsy is the removal of a small piece of tissue, under local anesthetic.