Study: NCT05301231

Intervention type: DIAGNOSTIC_TEST
Intervention: Genomics (DNA Extraction)
Description: Blood samples for detection of:~Genes for Dyslipidemia BY WES and NGS (4 GWA) (30 cases) Genes polymorphism for NAFLD/NASH BY TaqMan SNP Genotyping Assay on 10 GWAS

Intervention type: DIAGNOSTIC_TEST
Intervention: Genomics (DNA Extraction)
Description: Blood samples for detection of:~Genes for Dyslipidemia BY WES and NGS (4 GWA) (30 cases) Genes polymorphism for NAFLD/NASH BY TaqMan SNP Genotyping Assay on 10 GWAS

Intervention type: DIAGNOSTIC_TEST
Intervention: Genomics (DNA Extraction)
Description: Blood samples for detection of:~Genes for Dyslipidemia BY WES and NGS (4 GWA) (30 cases) Genes polymorphism for NAFLD/NASH BY TaqMan SNP Genotyping Assay on 10 GWAS

Intervention type: DIAGNOSTIC_TEST
Intervention: Epi-genomics
Description: * blood samples for detection of Expression profiling of plasma microRNAs~* PCR array to determine the altered miRNAs for 168 plasma miRNAs (12 cases)~* Expression analysis of top 5 altered miRNAs (All)

Intervention type: DIAGNOSTIC_TEST
Intervention: Epi-genomics
Description: * blood samples for detection of Expression profiling of plasma microRNAs~* PCR array to determine the altered miRNAs for 168 plasma miRNAs (12 cases)~* Expression analysis of top 5 altered miRNAs (All)

Intervention type: DIAGNOSTIC_TEST
Intervention: Epi-genomics
Description: * blood samples for detection of Expression profiling of plasma microRNAs~* PCR array to determine the altered miRNAs for 168 plasma miRNAs (12 cases)~* Expression analysis of top 5 altered miRNAs (All)

Intervention type: DIAGNOSTIC_TEST
Intervention: Proteomics (Glycoproteomics)
Description: blood samples for Identifying glycosylation pattern of five glycoproteins linked with NAFLD/NASH (transferrin, apoC III, haptoglobin, Mac2 binding protein, IgG)

Intervention type: DIAGNOSTIC_TEST
Intervention: Proteomics (Glycoproteomics)
Description: blood samples for Identifying glycosylation pattern of five glycoproteins linked with NAFLD/NASH (transferrin, apoC III, haptoglobin, Mac2 binding protein, IgG)

Intervention type: DIAGNOSTIC_TEST
Intervention: Proteomics (Glycoproteomics)
Description: blood samples for Identifying glycosylation pattern of five glycoproteins linked with NAFLD/NASH (transferrin, apoC III, haptoglobin, Mac2 binding protein, IgG)

Intervention type: DIAGNOSTIC_TEST
Intervention: Salivary Metabolomics
Description: Salivary Samples for detecting Salivary Metabolomics~1. Genome analysis (Identification of microbial strains common among Egyptians BY apid RT-PCR (DNA sequencing: 16S rRNA gene amplicons)~2. Microbiome related Metabolites Identification using GC-MS Analysis (the top 5 identified microbial-related metabolites) as metabolomics~3. Meta-genomic assess of three microbiome-related metabolites; lactoferrin, (LPS), (IgA) BY Commercial ELISA kits

Intervention type: DIAGNOSTIC_TEST
Intervention: Salivary Metabolomics
Description: Salivary Samples for detecting Salivary Metabolomics~1. Genome analysis (Identification of microbial strains common among Egyptians BY apid RT-PCR (DNA sequencing: 16S rRNA gene amplicons)~2. Microbiome related Metabolites Identification using GC-MS Analysis (the top 5 identified microbial-related metabolites) as metabolomics~3. Meta-genomic assess of three microbiome-related metabolites; lactoferrin, (LPS), (IgA) BY Commercial ELISA kits

Intervention type: DIAGNOSTIC_TEST
Intervention: Salivary Metabolomics
Description: Salivary Samples for detecting Salivary Metabolomics~1. Genome analysis (Identification of microbial strains common among Egyptians BY apid RT-PCR (DNA sequencing: 16S rRNA gene amplicons)~2. Microbiome related Metabolites Identification using GC-MS Analysis (the top 5 identified microbial-related metabolites) as metabolomics~3. Meta-genomic assess of three microbiome-related metabolites; lactoferrin, (LPS), (IgA) BY Commercial ELISA kits

Intervention type: BEHAVIORAL
Intervention: Individualized counselling for behavioural modification
Description: Individualized counselling for behavioral modification (3 sessions):~Nutritional education, Promotion of physical activities and Cognitive \& Psychological support

Intervention type: BEHAVIORAL
Intervention: Individualized counselling for behavioural modification
Description: Individualized counselling for behavioral modification (3 sessions):~Nutritional education, Promotion of physical activities and Cognitive \& Psychological support

Intervention type: BEHAVIORAL
Intervention: Individualized counselling for behavioural modification
Description: Individualized counselling for behavioral modification (3 sessions):~Nutritional education, Promotion of physical activities and Cognitive \& Psychological support