Study: NCT06539078

Intervention type: OTHER
Intervention: Maximal exercise test
Description: Participants will undertake an incremental ramp test on a cycle ergometer to determine maximal oxygen uptake (V̇O2max) and the gas exchange threshold (GET). Throughout the exercise test, muscle oxygenation and deoxygenation will be monitored by NIRS.

Intervention type: OTHER
Intervention: Maximal exercise test
Description: Participants will undertake an incremental ramp test on a cycle ergometer to determine maximal oxygen uptake (V̇O2max) and the gas exchange threshold (GET). Throughout the exercise test, muscle oxygenation and deoxygenation will be monitored by NIRS.

Intervention type: OTHER
Intervention: 3D Ultrasound
Description: Muscle volume and morphological characteristics will be assessed via 3D ultrasound imaging.

Intervention type: OTHER
Intervention: 3D Ultrasound
Description: Muscle volume and morphological characteristics will be assessed via 3D ultrasound imaging.

Intervention type: OTHER
Intervention: 3D Ultrasound
Description: Muscle volume and morphological characteristics will be assessed via 3D ultrasound imaging.

Intervention type: OTHER
Intervention: 3D Ultrasound
Description: Muscle volume and morphological characteristics will be assessed via 3D ultrasound imaging.

Intervention type: OTHER
Intervention: Dynamometry
Description: To determine the contractile properties of the knee extensors, participants will perform maximal isometric and isoinertial contractions of the knee extensors on a dynamometer.

Intervention type: OTHER
Intervention: Dynamometry
Description: To determine the contractile properties of the knee extensors, participants will perform maximal isometric and isoinertial contractions of the knee extensors on a dynamometer.

Intervention type: OTHER
Intervention: Dynamometry
Description: To determine the contractile properties of the knee extensors, participants will perform maximal isometric and isoinertial contractions of the knee extensors on a dynamometer.

Intervention type: OTHER
Intervention: Dynamometry
Description: To determine the contractile properties of the knee extensors, participants will perform maximal isometric and isoinertial contractions of the knee extensors on a dynamometer.

Intervention type: OTHER
Intervention: Exercise test and occlusions
Description: Participants will perform a series of moderate-intensity constant power output exercise bouts on a cycle ergometer following which the recovery rates of muscle V̇O2 will be determined via a series of intermittent arterial occlusions. Throughout all tests, pulmonary gas exchange and ventilation will be determined and muscle oxygenation and deoxygenation will be monitored by NIRS.

Intervention type: OTHER
Intervention: Exercise test and occlusions
Description: Participants will perform a series of moderate-intensity constant power output exercise bouts on a cycle ergometer following which the recovery rates of muscle V̇O2 will be determined via a series of intermittent arterial occlusions. Throughout all tests, pulmonary gas exchange and ventilation will be determined and muscle oxygenation and deoxygenation will be monitored by NIRS.

Intervention type: OTHER
Intervention: Exercise test and occlusions
Description: Participants will perform a series of moderate-intensity constant power output exercise bouts on a cycle ergometer following which the recovery rates of muscle V̇O2 will be determined via a series of intermittent arterial occlusions. Throughout all tests, pulmonary gas exchange and ventilation will be determined and muscle oxygenation and deoxygenation will be monitored by NIRS.

Intervention type: OTHER
Intervention: Exercise test and occlusions
Description: Participants will perform a series of moderate-intensity constant power output exercise bouts on a cycle ergometer following which the recovery rates of muscle V̇O2 will be determined via a series of intermittent arterial occlusions. Throughout all tests, pulmonary gas exchange and ventilation will be determined and muscle oxygenation and deoxygenation will be monitored by NIRS.

Intervention type: OTHER
Intervention: Exercise test in MRI
Description: Exercise will be performed on a custom-built magnetic resonance-compatible cycle ergometer in supine position for determination of muscle phosphocreatine recovery kinetics using 31phosphorous magnetic resonance spectroscopy \[31P-MRS\].

Intervention type: OTHER
Intervention: Exercise test in MRI
Description: Exercise will be performed on a custom-built magnetic resonance-compatible cycle ergometer in supine position for determination of muscle phosphocreatine recovery kinetics using 31phosphorous magnetic resonance spectroscopy \[31P-MRS\].

Intervention type: OTHER
Intervention: Exercise test in MRI
Description: Exercise will be performed on a custom-built magnetic resonance-compatible cycle ergometer in supine position for determination of muscle phosphocreatine recovery kinetics using 31phosphorous magnetic resonance spectroscopy \[31P-MRS\].

Intervention type: OTHER
Intervention: Exercise test in MRI
Description: Exercise will be performed on a custom-built magnetic resonance-compatible cycle ergometer in supine position for determination of muscle phosphocreatine recovery kinetics using 31phosphorous magnetic resonance spectroscopy \[31P-MRS\].

Intervention type: PROCEDURE
Intervention: Muscle biopsy
Description: A muscle biopsy will be obtained from the vastus lateralis using a modified Bergström needle technique with suction.

Intervention type: PROCEDURE
Intervention: Muscle biopsy
Description: A muscle biopsy will be obtained from the vastus lateralis using a modified Bergström needle technique with suction.

Intervention type: PROCEDURE
Intervention: Muscle biopsy
Description: A muscle biopsy will be obtained from the vastus lateralis using a modified Bergström needle technique with suction.

Intervention type: PROCEDURE
Intervention: Muscle biopsy
Description: A muscle biopsy will be obtained from the vastus lateralis using a modified Bergström needle technique with suction.