Study: NCT04992481

Intervention type: PROCEDURE
Group type: EXPERIMENTAL
Intervention: PMTC
Description: In patients from the study groups I, II, III, IV a professional mechanical tooth cleaning (PMTC) including air-polishing was proceeded on every visit using a glycine-based prophylaxis powder (Air-Flow Soft, EMS, Switzerland).

Intervention type: PROCEDURE
Group type: EXPERIMENTAL
Intervention: PMTC
Description: In patients from the study groups I, II, III, IV a professional mechanical tooth cleaning (PMTC) including air-polishing was proceeded on every visit using a glycine-based prophylaxis powder (Air-Flow Soft, EMS, Switzerland).

Intervention type: PROCEDURE
Group type: EXPERIMENTAL
Intervention: PMTC
Description: In patients from the study groups I, II, III, IV a professional mechanical tooth cleaning (PMTC) including air-polishing was proceeded on every visit using a glycine-based prophylaxis powder (Air-Flow Soft, EMS, Switzerland).

Intervention type: PROCEDURE
Group type: EXPERIMENTAL
Intervention: PMTC
Description: In patients from the study groups I, II, III, IV a professional mechanical tooth cleaning (PMTC) including air-polishing was proceeded on every visit using a glycine-based prophylaxis powder (Air-Flow Soft, EMS, Switzerland).

Intervention type: PROCEDURE
Group type: EXPERIMENTAL
Intervention: fluoride varnish
Description: In patients from groups I, II, III, IV a fluoride varnish of 5% NaF (Profluorid Varnish, Voco, Germany) was applied with a microbrush. Every patient was instructed to stop from drinking and eating for the next two hours (as recommended by the manufacturer).

Intervention type: PROCEDURE
Group type: EXPERIMENTAL
Intervention: fluoride varnish
Description: In patients from groups I, II, III, IV a fluoride varnish of 5% NaF (Profluorid Varnish, Voco, Germany) was applied with a microbrush. Every patient was instructed to stop from drinking and eating for the next two hours (as recommended by the manufacturer).

Intervention type: PROCEDURE
Group type: EXPERIMENTAL
Intervention: fluoride varnish
Description: In patients from groups I, II, III, IV a fluoride varnish of 5% NaF (Profluorid Varnish, Voco, Germany) was applied with a microbrush. Every patient was instructed to stop from drinking and eating for the next two hours (as recommended by the manufacturer).

Intervention type: PROCEDURE
Group type: EXPERIMENTAL
Intervention: fluoride varnish
Description: In patients from groups I, II, III, IV a fluoride varnish of 5% NaF (Profluorid Varnish, Voco, Germany) was applied with a microbrush. Every patient was instructed to stop from drinking and eating for the next two hours (as recommended by the manufacturer).

Intervention type: PROCEDURE
Group type: EXPERIMENTAL
Intervention: gaseous ozone therapy
Description: Patients from groups II and IV were additionally subjected to in-office ozone therapy using OzonyTron-OZ (Mymed, Germany). Ozone was applied on a silicone double-jaw tray of a proper size (allowing even ozone distribution in the oral cavity) following professional mechanical tooth cleaning. A Full Mouth Desinfection program lasting 5 minutes was used.

Intervention type: PROCEDURE
Group type: EXPERIMENTAL
Intervention: gaseous ozone therapy
Description: Patients from groups II and IV were additionally subjected to in-office ozone therapy using OzonyTron-OZ (Mymed, Germany). Ozone was applied on a silicone double-jaw tray of a proper size (allowing even ozone distribution in the oral cavity) following professional mechanical tooth cleaning. A Full Mouth Desinfection program lasting 5 minutes was used.

Intervention type: PROCEDURE
Group type: EXPERIMENTAL
Intervention: domestic octenidin mouth rinse
Description: Patients from groups III and IV received a domestic mouthrinse (Octenidol MD, Schülke, Germany) and were recommended to use 15 ml of the liquid for 30 seconds after evening toothbrushing.

Intervention type: PROCEDURE
Group type: EXPERIMENTAL
Intervention: domestic octenidin mouth rinse
Description: Patients from groups III and IV received a domestic mouthrinse (Octenidol MD, Schülke, Germany) and were recommended to use 15 ml of the liquid for 30 seconds after evening toothbrushing.