Study: NCT02831374

Intervention type: DRUG
Group type: EXPERIMENTAL
Intervention: Local anesthesia
Description: The surgical extraction under local anesthesia (2% Lignocaine and 1:100000 Adrenaline) procedure was the same in all patients.

Intervention type: DRUG
Group type: PLACEBO_COMPARATOR
Intervention: Local anesthesia
Description: The surgical extraction under local anesthesia (2% Lignocaine and 1:100000 Adrenaline) procedure was the same in all patients.

Intervention type: PROCEDURE
Group type: EXPERIMENTAL
Intervention: surgical extraction of impacted third molar
Description: After achieving Local anesthesia using 2% lignocaine hydrochloride with 1:100,000 epinephrine, standard Terrence Ward's incision was placed to raise a full thickness mucoperiosteal flap. Disto-buccal bone was carefully exposed and bone surrounding the buccal aspect of impacted tooth was performed using surgical bur under copious irrigation with 0.9% saline. Tooth was elevated using Coupland elevator and if required situations tooth was sectioned with surgical bur to facilitate its removal. The socket was checked for any tooth or bony debris. The bony margins smoothened using a file and irrigated with 0.9% saline.

Intervention type: PROCEDURE
Group type: PLACEBO_COMPARATOR
Intervention: surgical extraction of impacted third molar
Description: After achieving Local anesthesia using 2% lignocaine hydrochloride with 1:100,000 epinephrine, standard Terrence Ward's incision was placed to raise a full thickness mucoperiosteal flap. Disto-buccal bone was carefully exposed and bone surrounding the buccal aspect of impacted tooth was performed using surgical bur under copious irrigation with 0.9% saline. Tooth was elevated using Coupland elevator and if required situations tooth was sectioned with surgical bur to facilitate its removal. The socket was checked for any tooth or bony debris. The bony margins smoothened using a file and irrigated with 0.9% saline.

Intervention type: BIOLOGICAL
Group type: EXPERIMENTAL
Intervention: Preparation of PRP gel
Description: Before surgery, 10 milliliter of venous blood was collected from the anticubital fossa of the patients forearm and stored into sterile tubes containing anticoagulant (0.5 milliliter citrate phosphate dextrose) from each patient in the experimental group using routine venipuncture procedure. The whole blood is then centrifuged at 1,200 rotations per minute for ten minutes. The supernatant layer obtained was platelet poor plasma and buffy coat was collected in a new sterile tube and centrifuged again at 1,000 rotations per minute for 10 min. The upper half of the supernatant is removed and the lower half is mixed carefully to get platelet rich plasma. Five drops of mixture of 10 % CaCl2 and 1000 Units bovine thrombin to 2 milliliter Platelet rich plasma to obtain the gel form.

Intervention type: PROCEDURE
Group type: EXPERIMENTAL
Intervention: Placing platelet Rich plasma and suturing
Description: Subsequently, platelet rich plasma gel was placed into the extraction sockets of patients in the group A and wound closure was done using simple interrupted 3.0 black silk sutures.

Intervention type: PROCEDURE
Group type: PLACEBO_COMPARATOR
Intervention: Suturing
Description: Wound closure was done using simple interrupted 3.0 black silk sutures.

Intervention type: DRUG
Group type: EXPERIMENTAL
Intervention: Postoperative medication
Description: Patients were advised regular post extraction and prescribed 500 milligram of paracetemol postoperatively (1 tablet every 6 hours for 2 days).

Intervention type: DRUG
Group type: PLACEBO_COMPARATOR
Intervention: Postoperative medication
Description: Patients were advised regular post extraction and prescribed 500 milligram of paracetemol postoperatively (1 tablet every 6 hours for 2 days).