Study: NCT03388359

Intervention type: PROCEDURE
Group type: EXPERIMENTAL
Intervention: Bronchial challenge with allergen
Description: Bronchial challenge is performed with D. pteronyssinus allergen. Measurements of differences in eosinophils activity after allergen challenge.

Intervention type: PROCEDURE
Group type: ACTIVE_COMPARATOR
Intervention: Bronchial challenge with allergen
Description: Bronchial challenge is performed with D. pteronyssinus allergen. Measurements of differences in eosinophils activity after allergen challenge.

Intervention type: OTHER
Group type: EXPERIMENTAL
Intervention: Co-culture formation
Description: Eosinophil and linear bronchial smooth muscle cell or pulmonary fibroblast co-culture formation. Bronchial smooth muscle cell and pulmonary fibroblast proliferation, migration, contractillity, differentiation, eosinophil adhesion to the bronchial smooth muscle cells or pulmonary fibroblast.

Intervention type: OTHER
Group type: ACTIVE_COMPARATOR
Intervention: Co-culture formation
Description: Eosinophil and linear bronchial smooth muscle cell or pulmonary fibroblast co-culture formation. Bronchial smooth muscle cell and pulmonary fibroblast proliferation, migration, contractillity, differentiation, eosinophil adhesion to the bronchial smooth muscle cells or pulmonary fibroblast.

Intervention type: OTHER
Group type: EXPERIMENTAL
Intervention: Inhibition of Wnt and Smad signaling pathways
Description: Wnt and Smad signaling pathways inhibitors effect on development of airway remodelling processes (extracellular matrix production, bronchial smooth muscle cell and pulmonary fibroblast proliferation, contractillity, differentiation, migration).

Intervention type: OTHER
Group type: ACTIVE_COMPARATOR
Intervention: Inhibition of Wnt and Smad signaling pathways
Description: Wnt and Smad signaling pathways inhibitors effect on development of airway remodelling processes (extracellular matrix production, bronchial smooth muscle cell and pulmonary fibroblast proliferation, contractillity, differentiation, migration).

Intervention type: OTHER
Group type: EXPERIMENTAL
Intervention: Extracellular matrix turnover and deposition assessment
Description: Eosinophils effect on extracellular matrix proteins (collagen, fibronectin, elastin, versican, decorin, laminin, etc.) and matrix metalloproteinasis (MMP-2,9,12,etc.) production by pulmonary fibroblasts.

Intervention type: OTHER
Group type: ACTIVE_COMPARATOR
Intervention: Extracellular matrix turnover and deposition assessment
Description: Eosinophils effect on extracellular matrix proteins (collagen, fibronectin, elastin, versican, decorin, laminin, etc.) and matrix metalloproteinasis (MMP-2,9,12,etc.) production by pulmonary fibroblasts.

Intervention type: BIOLOGICAL
Group type: EXPERIMENTAL
Intervention: Dermatophagoides pteronyssinus allergen
Description: Dermatophagoides pteronyssinus allergen is required to perform allergen bronchial challenge test.

Intervention type: BIOLOGICAL
Group type: ACTIVE_COMPARATOR
Intervention: Dermatophagoides pteronyssinus allergen
Description: Dermatophagoides pteronyssinus allergen is required to perform allergen bronchial challenge test.

Intervention type: DEVICE
Group type: EXPERIMENTAL
Intervention: Dosimeter ProvoX (Ganshorn)
Description: Device for allergen bronchial challenge test.