Please select the eligibility criteria that correspond to the clinical trial below.
Title: The Impact of Intermittent Fasting on Human Metabolism and Cell Autophagy
Brief Summary: InterFast is a Cohort study with an embedded randomized controlled pilot trial. Study participants will be healthy subjects and subjects who already practice Alternate Day Fasting. The trial will include 100 participants (50 Participants in Alternate Day Fasting group and 50 participants in the control group). Those participants in the control group will be asked to participate in a short randomized controlled trial, where they will be either allocated to an Alternative Day Fasting group or another control visit.


Options:
A: Inclusion Criteria:

* Females;
* Aged between 18 and 59 years old;
* Body mass index equal or greater 25 kg/m²;
* Agree to sign the informed consent.

Exclusion Criteria:

* Any chronic health conditions (e.g. chronic renal failure, autoimmune, heart, and liver disease);
* Thyroid diseases or medications for its treatment;
* Medications known to affect total energy expenditure;
* Use of steroids, immunosuppressants or non-steroidal anti-inflammatory drugs, weight-loss medications, oral hypoglycemic agents or insulin and any medications that influence metabolic and/or inflammatory parameters;
* Previous surgery for weight loss;
* Pregnancy or breast-feeding;
* Smoking;
* Alcohol use (\>2 doses/day).
B: Inclusion Criteria:

* Body mass index in the range of 22.0 - 27.0 kg/m2,
* Fasting blood glucose \<110mg/dL (without medication)
* LDL-cholesterol \<180 mg/dL (without medication)
* Blood pressure \<140/90 mmHg (without medication)
* Stable weight (change \<± 10%) for 3 months immediately prior to the study,
* No history of metabolic disorders or cardiovascular disease
* No acute or chronic inflammatory disorder
* No current medications to regulate blood sugar, blood pressure or lipids or hormones
* No heavy drinking (more than 15 drinks/week)
* No use of tobacco or recreational drugs within past 5 years
* No dietary restrictions (e.g. vegetarianism and vegan)

Exclusion Criteria:

* Known Malignancy
* Women who are pregnant, breast-feeding or trying to become pregnant
* History of any chronic disease process that could interfere with interpretation of study results
* Women or men on hormonal supplementation or anti-conceptive hormonal medication for at least 2 months
* Therapy with antidepressants within past 6 months
* Regular therapy with acetylsalicylic acid
C: Inclusion Criteria:

* Age ≥ 18 years
* BMI≥24kg/m2
* Polycystic ovary syndrome has been diagnosed

Exclusion Criteria:

* Taking medications affecting weight or energy intake/energy expenditure in the last 6 months, including weight loss medications, antipsychotic drugs or other medications as determined by the study physician;
* The body weight fluctuated more than 5% in recent 3 months.
* Liver and kidney dysfunction: renal impairment, creatinine clearance rate \< 30 mL/min/1.73 m2, transaminase increased, more than three times higher than the normal limit;
* History of serious cardiovascular or cerebrovascular disease (angina, myocardial infarction or stroke) in the past 6 months;
* History of thyroid diseases;
* Having been in pregnancy.
* Researchers believe that there are any factors that affect assessing subjects' participation in trial.
* History of malignant tumors;
* History of Cushing's syndrome, hypothyroidism, acromegaly, hypothalamic obesity;
* Currently participating in weight loss programs or weight change in the past 3 months (\> 5% current body weight)
* Patients who cannot be followed for 16 months (due to a health situation or migration)
* Patients who are unwilling or unable to give informed consent.
D: Inclusion Criteria:

* The participants in this study will be 50 men and women in the 30 to 65 year age range, who have a BMI in the high normal to moderately obese range (i.e. 24 to 35 kg/m2),
* Participants who are eating usual US diets and are sedentary to moderately active (i.e. not exercise trained).

Exclusion Criteria:

* History of any chronic disease process that could interfere with interpretation of results
* Smoking, pregnancy, alcoholism, psychiatric problems, life situations that would interfere with study participation and compliance.

Answer: B