Please select the eligibility criteria that correspond to the clinical trial below.
Title: Effectiveness of PGT-A by Using IVF Versus ICSI for Non-male Factor Infertility: a Pilot Study
Brief Summary: The investigators want to investigate if the use of conventional in vitro fertilization (IVF) can generate more euploid embryos as compared to intracytoplasmic sperm injection (ICSI).

To verify this, the investigators will inject half of the oocytes by ICSI and the other half of the oocytes will be inseminated by conventional IVF. The most important aim is to see if IVF is able to generate more euploid embryos.


Options:
A: Inclusion Criteria:

* Having ≤ 2 IVF/ICSI cycles
* Total sperm count and motility are normal (WHO, 2010)
* Antagonist protocol
* Agree to have ≤ 2 embryos transferred
* Not participating in another IVF study at the same time

Exclusion Criteria:

* In-vitro maturation (IVM) cycles
* Using frozen semen
* Poor fertilization in previous cycle (≤ 25%)
B: Inclusion Criteria:

* Infertile couples scheduled for their first or second IVF/ICSI cycle.
* Men with non-severe male infertility: Sperm concentration 5,000,000-15,000,000/ml or sperm with progressive motility (type A+B) 10-32%.
* Women received either gonadotrophin-releasing hormone agonist protocol or gonadotrophin-releasing hormone antagonist protocol as their controlled ovarian hyperstimulation treatment.
* Informed consent obtained.

Exclusion Criteria:

* Couple with contraindication for IVF or ICSI.
* Couples receiving donor sperm or donor eggs.
* Couples undergoing PGD and PGS.
* Sperm concentration with progressive motility used for insemination \<100,000/ml on the day of oocyte retrieval.
* Women with 0 oocytes after oocyte retrieval.
* Using frozen semen.
* Poor fertilization in previous cycle (≤ 25%).
C: Inclusion Criteria:

* Women 18-42 years of age (both included) at the beginning of the ovarian stimulation
* BMI 18-35 kg/m2
* Indication for IVF due to tubal factor infertility, unexplained infertility, PCOS or light to moderate decreased semen quality
* Women treated with gonadotrophin in a standard short or long protocol and receiving ovulation trigger for oocyte pick up
* First treatment cycle for the couple
* Male partner with normal or non-severely decreased sperm parameters, where the sperm sample (purified) on the day of oocyte pick up is expected to contain a minimum of 2 million/mL progressive motile spermatozoa. Alternatively use of donorsperm.
* Willing to sign the informed consent

Exclusion Criteria:

* Ovarian cysts \>4 cm
* Known liver or kidney disease
* Previous IVF or ICSI treatment with current partner
* Use of donor oocytes or frozen oocytes
* Unregulated thyroid disease
* Endometriosis stage 3-4
* Hypogonadotropic hypogonadism
* Severe comorbidity (e.g. diabetes or cardiovascular disease)
* Not speaking / understanding Danish or English language
* Not willing to sign the informed consent
D: Inclusion Criteria:

* Sperm parameters Sperm concentration before capacitation: \>15\*106 per ml (WHO) Total motility (PR+NP,%): \>40 (WHO) Progressive motility (PR,%):\>32 (WHO) Sperm concentration after capacitation: \>0.6\*106 per ml (not WHO defined) Progressive motility (PR,%):\>65 (WHO)
* ≥10 COCs after oocyte retrieval
* BMI ≤30 kg/m2
* Female age 18 to ≤ 40 years
* All ovarian stimulation protocols
* Fresh ejaculates
* Presence or absence of sperm morphology data: as the investigators do not have a diagnostic sperm analysis for all participants, the presence or absence of \>4% normal morphology (WHO) will not be taken into account, even with known low (\<4%) normal morphology
* Couples requesting PGT-A
* Arab population

Exclusion Criteria:

* If after denudation (ICSI) only 2 mature oocytes are obtained
* If all time lapse spaces are occupied
* If the volume to be added after IVF is insufficient to perform IVF on all needed oocytes
* Presence of \>1 \*106 per ml round cells in the ejaculate
* If a couple's previous cycle was included in the study

Answer: D