prompt_front: Answer the question based on the given passages. Only give me the answer and do not output any other words.

Question: Which is a flowering plant, Pueraria or Pleiospilos?

The following are given passages.
Context 1: Passage 1:
Pueraria
Pueraria is a genus of 15–20 species of legumes native to Asia  The best known member is kudzu, also called Japanese arrowroot  The genus is named after 19th century Swiss botanist Marc Nicolas Puerari 
The genus, as traditionally circumscribed, is polyphyletic, with different species being more related to other species in the tribe Phaseoleae  Current research, reproduced below, splits the genus into five clades, one of which defines the current monophyletic genus 

Species
The genus Pueraria is highly polyphyletic; the below list is divided by clade following the result of A N Egan & B Pan (2016)  In 2015, the authors validly published their proposal in Phytotaxa  As of February 2022, Kew Plants of the World Online database accepts these names 

Pueraria sensu stricto
Pueraria sensu stricto includes the vast majority of species in the genus  They fall into a single clade sister to or containing Nogra 
P  alopecuroides Craib
P  calycina Franch 
P  candollei  Benth 
P  edulis Pamp 
P  imbricata Maesen
P  lacei Craib
P  mirifica Airy Shaw & Suvat  (= P  candollei var  mirifica in Egan)
P  montana (Lour ) Merr  – US invasive population comes from a hybrid of more than one of the subspecies Pueraria montana var  chinensis (Ohwi) Sanjappa & Pradeep (= P  chinensis, although ILDS and the plant list instead consider P  thomsonii the synonym)
Pueraria montana var  lobata (Willd ) Sanjappa & Pradeep (= P  lobata)
Pueraria montana var  thomsonii (Benth ) Wiersema ex D B  Ward (= P  thomsonii, missing in ILDS)
P  pulcherrima (Koord ) Koord -Schum 
P  sikkimensis Prain
P  tuberosa (Roxb ex Willd ) DC  – type species

Provisionally retained
The following are not included in the 2016 study due to insufficient material for sequencing  They are accepted by POWO 
P  bella Prain: conflicting proposals assigning either to the main clade or to Neonotonia (morphology) 
P  bouffordii H  Ohashi: presumably in the main clade (morphology) 
P  grandiflora Bo Pan & Bing Liu: presumably in the main clade (morphology) 
P  xyzhuii H  Ohashi & Iokawa: presumably in the main clade (morphology) The following are not included in Egan et al  2016 for other reasons, but are accepted by Kew POWO:

P  garhwalensis L R Dangwal & D S Rawat: excluded per van der Maesen (2002)
P  neocaledonica Harms: not mentioned
P  maesenii Niyomdham: not mentioned

Former members
The rest of the genus fall into four clades, sorted by distance from the main clade:
Neustanthus Benth  – sister to Sinodolichos
P  phaseoloides (Roxb ) Benth  → N  phaseoloides (Roxb ) Benth P  edulis, P  montana, and N  phaseoloides make up what is known as kudzu  The morphological differences between these species are subtle 
N  phaseoloides var  javanicus (= P  javanica (Benth ) Benth )
N  phaseoloides var  phaseoloides
N  phaseoloides var  subspicatus
Teyleria
P  stricta Kurz → T  stricta
Toxicopueraria A N Egan & B Pan – sister to Cologania
P  peduncularis Grah  → T  peduncularis
P  yunnanensis Franchet → T  yunnanensis
Haymondia A N Egan & B Pan – notably lies out of Glycininae near Kennediinae; known for a long time to be misplaced
P  wallichii DC  → H  wallichiiThe following names are not accepted even before Egan 2016 but have seen valid publication:

P  omeiensis Wang et Tang – P  montana: unaccepted name after Mount Omei 
P  stracheyi Baker → Apios carnea (Wall ) Benth  ex Baker 
P  maclurei (F  P  Metcalf) F  J  Herm  → Sinodolichos lagopus – still accepted by WFO
Passage 2:
Pleiospilos nelii
Pleiospilos nelii, the split rock, splitrock or living granite, is a species of flowering plant in the family Aizoaceae, native to South Africa  It grows in semi-arid areas with rainfall of between 150mm and 300mm, in the Karoo of South Africa 

Etymology
The species epithet nelii honours the South African botanist Gert Cornelius Nel  The common name "split rock" refers to the appearance of the plant's leaves 

Description
Pleiospilos nelii is a succulent perennial which can reach a height of 5–8 cm (2–3 in) and a diameter of about 10 cm (4 in)  This very short-stemmed, nearly stemless plant has one or more pairs of opposite, almost hemispherical, grey-green or brownish leaves  The surface of the leaves has many small dark spots  A new pair of leaves is produced each year, replacing an older pair  This plant closely resembles a small cracked rock (hence the common name), an appearance which may have evolved as a defence against herbivory  The superficially daisy-like flowers are yellow-orange, 6–7 5 cm (2–3 in) across, and emerge from the plant's apical bud  The flowers are large in relation to the size of the plant  Each flower opens in the afternoon and closes at sunset, over a bloom period of several days 

Cultivation
P  nelii is one of several plants cultivated for their rocklike appearance  They are sometimes collectively called mesembs  In temperate regions it is grown under glass for protection from excessive rain and hard freezes  It has gained the Royal Horticultural Society's Award of Garden Merit 

Gallery
Passage 3:
Pleiospilos simulans
Pleiospilos simulans (liver plant, split rock plant) is a species of flowering plant in the family Aizoaceae, once endemic to the Eastern Cape of South Africa  Known populations of the plant in the wild are now extinct due its collection as an ornamental plant for succulent collectors  It is a small, low-growing, perennial with succulent leaves and orange or yellow many-petaled flowers 
Passage 4:
Pleiospilos
Pleiospilos is a genus of succulent flowering plants of the family Aizoaceae, native to South Africa  The name is derived from the Greek pleios "many" and spìlos "spot"  The plants are also known as kwaggavy ("Quagga mesemb"), lewerplant ("liver plant"), lewervygie ("liver mesemb"), klipplant ("stone plant"), split rock or mimicry plant 
Pleiospilos species have two or four opposite, very fleshy, grey-green leaves growing from a short stem that may be underground  The leaves, with their hemispherical shape and pitted texture, often strongly resemble a pile of split pebbles to deter predators  Flowers emerge from the center of the leaves, and may be very big in relation to the overall size of the plant They reproduce both sexually and asexually  Vegetative offshoots emerge from the root system  Seed set occurs in early spring around March The species P  bolusii and  P  nelii are cultivated as ornamentals 

Species
Pleiospilos bolusii
Pleiospilos compactus
P  compactus subsp  compactus
P  compactus subsp  canus
P  compactus subsp  minor
P  compactus subsp  fergusoniae
P  compactus subsp  sororius
Pleiospilos nelii
Pleiospilos simulans
Passage 5:
Pleiospilos bolusii
Pleiospilos bolusii, the mimicry plant, is a species of flowering plant in the family Aizoaceae, native to the Eastern Cape of South Africa, where it grows at an altitude of 750–1,100 m (2,461–3,609 ft)  The species epithet bolusii honours Harry Bolus, a 19th-century South African botanist 
It is a small, stemless succulent perennial growing to 8 cm (3 in) tall by 15 cm (6 in) wide, with two or four opposite grey-green leaves, quite thick, fused at the base, almost triangular, with entire margins  The leaves are longer and more angular than those of the closely related P  nelii, but in both cases the shape and texture of the leaves resemble a pile of split pebbles, possibly to deter predators  The yellow, many-petaled, daisy-like flowers emerge from the center of the leaves, and are 6–8 cm (2–3 in) in diameter - large in relation to the overall size of the plant  The flowers are often coconut-scented  The flowering period extends from August to September 
The plant is one of several species cultivated for their rocklike appearance, which are sometimes collectively called mesembs  With a minimum temperature of 7 °C (45 °F), in temperate regions it is grown under glass  It has gained the Royal Horticultural Society's Award of Garden Merit 



ground_truth: Pleiospilos

prompt_front: Answer the question based on the given passages. Only give me the answer and do not output any other words.

Question: The large subunit and small subunit that use two types of RNA are major components that make up what?

The following are given passages.
Context 2: Passage 1:
Short interspersed nuclear elements (SINEs)
Short interspersed nuclear elements (SINEs) are non-autonomous, non-coding transposable elements (TEs) that are about 100 to 700 base pairs in length  They are a class of retrotransposons, DNA elements that amplify themselves throughout eukaryotic genomes, often through RNA intermediates  SINEs compose about 13% of the mammalian genome The internal regions of SINEs originate from tRNA and remain highly conserved, suggesting positive pressure to preserve structure and function of SINEs   While SINEs are present in many species of vertebrates and invertebrates, SINEs are often lineage specific, making them useful markers of divergent evolution between species  Copy number variation and mutations in the SINE sequence make it possible to construct phylogenies based on differences in SINEs between species  SINEs are also implicated in certain types of genetic disease in humans and other eukaryotes 
In essence, short interspersed nuclear elements are genetic parasites which have evolved very early in the history of eukaryotes to utilize protein machinery within the organism as well as to co-opt the machinery from similarly parasitic genomic elements  The simplicity of these elements make them remarkably successful at persisting and amplifying (through retrotransposition)  within the genomes of eukaryotes  These "parasites" which have become ubiquitous in genomes can be very deleterious to organisms as discussed below  However, eukaryotes have been able to integrate short-interspersed nuclear elements into different signaling, metabolic and regulatory pathways and SINEs have become a great source of genetic variability  They seem to play a particularly important role in the regulation of gene expression and the creation of RNA genes  This regulation extends to chromatin re-organization and the regulation of genomic architecture  The different lineages, mutations, and activities among eukaryotes make short-interspersed nuclear elements a useful tool in phylogenetic analysis 

Classification and structure
SINEs are classified as non-LTR retrotransposons because they do not contain long terminal repeats (LTRs)  There are three types of SINEs common to vertebrates and invertebrates: CORE-SINEs, V-SINEs, and AmnSINEs  SINEs have 50-500 base pair internal regions which contain a tRNA-derived segment with A and B boxes that serve as an internal promoter for RNA polymerase III 

Internal structure
SINEs are characterized by their different modules, which are essentially a sectioning of their sequence  SINEs can, but do not necessarily have to possess a head, a body, and a tail  The head, is at the 5' end of short-interspersed nuclear elements and is an evolutionarily derived from an RNA synthesized by RNA Polymerase III such as ribosomal RNAs and tRNAs; the 5' head is indicative of which endogenous element that SINE was derived from and was able to parasitically utilize its transcriptional machinery  For example, the 5' of the Alu sine is derived from 7SL RNA, a sequence transcribed by RNA Polymerase III which codes for the RNA element of SRP, an abundant ribonucleoprotein  The body of SINEs possess an unknown origin but often share much homology with a corresponding LINE which thus allows SINEs to parasitically co-opt endonucleases coded by LINEs (which recognize certain sequence motifs)  Lastly, the 3′ tail of SINEs is composed of short simple repeats of varying lengths; these simple repeats are sites where two (or more) short-interspersed nuclear elements can combine to form a dimeric SINE  Short-interspersed nuclear elements which only possess a head and tail are called simple SINEs whereas short-interspersed nuclear elements which also possess a body or are a combination of two or more SINEs are complex SINEs 

Transcription
Short-interspersed nuclear elements are transcribed by RNA polymerase III which is known to transcribe ribosomal RNA and tRNA, two types of RNA vital to ribosomal assembly and mRNA translation  SINEs, like tRNAs and many small-nuclear RNAs possess an internal promoter and thus are transcribed differently than most protein-coding genes   In other words, short-interspersed nuclear elements have their key promoter elements within the transcribed region itself  Though transcribed by RNA polymerase III, SINEs and other genes possessing internal promoters, recruit different transcriptional machinery and factors than genes possessing upstream promoters 

Effects on gene expression
Changes in chromosome structure influence gene expression primarily by affecting the accessibility of genes to transcriptional machinery  The chromosome has a very complex and hierarchical system of organizing the genome  This system of organization, which includes histones, methyl groups, acetyl groups, and a variety of proteins and RNAs allows different domains within a chromosome to be accessible to polymerases, transcription factors, and other associated proteins to different degrees   Furthermore, the shape and density of certain areas of a chromosome can affect the shape and density of neighboring (or even distant regions) on the chromosome through interaction facilitated by different proteins and elements  Non-coding RNAs  such as short-interspersed nuclear elements, which have been known to associate with and contribute to chromatin structure, can thus play huge role in regulating gene expression   Short-interspersed-nuclear-elements similarly can be involved in gene regulation by modifying genomic architecture 
In fact Usmanova et al  2008 suggested that short-interspersed nuclear elements can serve as direct signals in chromatin rearrangement and structure   The paper examined the global distribution of SINEs in mouse and human chromosomes and determined that this distribution was very similar to genomic distributions of genes and CpG motifs  The distribution of SINEs to genes was significantly more similar than that of other non-coding genetic elements and even differed significantly from the distribution of long-interspersed nuclear elements  This suggested that the SINE distribution was not a mere accident caused by LINE-mediated retrotransposition but rather that SINEs possessed a role in gene-regulation  Furthermore, SINEs frequently contain motifs for YY1 polycomb proteins  YY1 is a zinc-finger protein that acts as a transcriptional repressor for a wide-variety of genes essential for development and signaling   Polycomb protein YY1 is believed to mediate the activity of histone deacetylases and histone acetyltransferases to facilitate chromatin re-organization; this is often to facilitate the formation of heterochromatin (gene-silencing state)  Thus, the analysis suggests that short-interspersed nuclear elements can function as a ‘signal-booster' in the polycomb-dependent silencing of gene-sets through chromatin re-organization   In essence, it is the cumulative effect of many types of interactions that leads to the difference between euchromatin, which is not tightly packed and generally more accessible to transcriptional machinery, and heterochromatin, which is tightly packed and generally not accessible to transcriptional machinery; SINEs seem to play an evolutionary role in this process 
In addition to directly affecting chromatin structure, there are a number of ways in which SINEs can potentially regulate gene expression  For example, long non-coding RNA can directly interact with transcriptional repressors and activators, attenuating or modifying their function   This type of regulation can occur in different ways: the RNA transcript can directly bind to the transcription factor as a co-regulator; also, the RNA can regulate and modify the ability of co-regulators to associate with the transcription factor   For example, Evf-2, a certain long non-coding RNA, has been known to function as a co-activator for certain homeobox transcription factors which are critical to nervous system development and organization  Furthermore, RNA transcripts can interfere with the functionality of the transcriptional complex by interacting or associating with RNA polymerases during the transcription or loading processes  Moreover, non-coding RNAs like SINEs can bind or interact directly with the DNA duplex coding the gene and thus prevent its transcription Also, many non-coding RNAs are distributed near protein-coding genes, often in the reverse direction  This is especially true for short-interspersed nuclear elements as seen in Usmanova et al  These non-coding RNAs, which lie adjacent to or overlap gene-sets provide a mechanism by which transcription factors and machinery can be recruited to increase or repress the transcription of local genes  The particular example of SINEs potentially recruiting the YY1 polycomb transcriptional repressor is discussed above   Alternatively, it also provides a mechanism by which local gene expression can be curtailed and regulated because the transcriptional complexes can hinder or prevent nearby genes from being transcribed  There is research to suggest that this phenomenon is particularly seen in the gene-regulation of pluripotent cells In conclusion, non-coding RNAs such as SINEs are capable of affecting gene expression on a multitude of different levels and in different ways  Short-interspersed nuclear elements are believed to be deeply integrated into a complex regulatory network capable of fine-tuning gene expression across the eukaryotic genome 

Propagation and regulation
The RNA coded by the short-interspersed nuclear element does not code for any protein product but is nonetheless reverse-transcribed and inserted back into an alternate region in the genome  For this reason, short interspersed nuclear elements are believed to have co-evolved with long interspersed nuclear element (LINEs), as LINEs do in fact encode protein products which enable them to be reverse- transcribed and integrated back into the genome   SINEs are  believed to have co-opted the proteins coded by LINEs which are contained in 2 reading frames  Open reading frame 1 (ORF 1)  encodes a protein which binds to RNA and acts as a chaperone to facilitate and maintain the LINE protein-RNA complex structure  Open reading frame 2 (ORF 2) codes a protein which possesses both endonuclease and reverse transcriptase activities  This enables the LINE mRNA to be reverse-transcribed into DNA and integrated into the genome based on the sequence-motifs recognized by the protein's endonuclease domain 
LINE-1 (L1) is transcribed and retrotransposed most frequently in the germ-line and during early development; as a result SINEs move around the genome most during these periods  SINE transcription is down-regulated by transcription factors in somatic cells after early development, though stress can cause up-regulation of normally silent SINEs  SINEs can be transferred between individuals or species via horizontal transfer through a viral vector SINEs are known to share sequence homology with LINES which gives a basis by which the LINE machinery can reverse transcribe and integrate SINE transcripts  Alternately, some SINEs are believed to use a much more complex system of integrating back into the genome; this system involves the use random double-stranded DNA breaks (rather than the endonuclease coded by related long-interspersed nuclear elements creating an insertion-site)  These DNA breaks are utilized to prime reverse transcriptase, ultimately integrating the SINE transcript back into the genome  SINEs nonetheless depend on enzymes coded by other DNA elements and are thus known as non-autonomous retrotransposons as they depend on the machinery of LINEs, which are known as autonomous retrotransposons <The theory that short-interspersed nuclear elements have evolved to utilize the retrotransposon machinery of long-interspersed nuclear elements is supported by studies which examine the presence and distribution of LINEs and SINEs in taxa of different species  For example, LINEs and SINEs in rodents and primates show very strong homology at the insertion-site motif  Such evidence is a basis for the proposed mechanism in which integration of the SINE transcript can be co-opted with LINE-coded protein products  This is specifically demonstrated by a detailed analysis of over 20 rodent species profiled LINEs and SINEs, mainly L1s and B1s respectively; these are families of LINEs and SINEs found at high frequencies in rodents along with other mammals  The study sought to provide phylogenetic clarity within the context of LINE and SINE activity 
The study arrived at a candidate taxa believed to be the first instance of L1 LINE extinction; it expectedly discovered that there was no evidence to suggest that B1 SINE activity occurred in species which did not have L1 LINE activity  Also, the study suggested that B1 short-interspersed nuclear element silencing in fact occurred before L1 long-interspersed nuclear element extinction; this is due to the fact that B1 SINEs are silenced in the genus most-closely related to the genus which does not contain active L1 LINEs (though the genus with B1 SINE silencing still contains active L1 LINEs)   Another genus was also found which similarly contained active L1 long-interspersed nuclear elements but did not contain B1 short-interspersed nuclear elements; the opposite scenario, in which active B1 SINEs were present in a genus which did not possess active L1 LINEs was not found  This result was expected and strongly supports the theory that SINEs have evolved to co-opt the RNA-binding proteins, endonucleases, and reverse-transcriptases coded by LINEs  In taxa which do not actively transcribe and translate long-interspersed nuclear elements protein-products, SINEs do not have the theoretical foundation by which to retrotranspose within the genome  The results obtained in Rinehart et al  are thus very supportive of the current model of SINE retrotransposition 

Effects of SINE transposition
Insertion of a SINE upstream of a coding region may result in exon shuffling or changes to the regulatory region of the gene  Insertion of a SINE into the coding sequence of a gene can have deleterious effects and unregulated transposition can cause genetic disease  The transposition and recombination of SINEs and other active nuclear elements is thought to be one of the major contributions of genetic diversity between lineages during speciation 

Common SINEs
Short-interspersed nuclear elements are believed to have parasitic origins in eukaryotic genomes  These SINEs have mutated and replicated themselves a large number of times on an evolutionary time-scale and thus form many different lineages  Their early evolutionary origin has caused them to be ubiquitous in many eukaryotic lineages 
Alu elements, short-interspersed nuclear element of about 300 nucleotides, are the most common SINE in humans, with >1,000,000 copies throughout the genome, which is over 10 percent of the total genome; this is not uncommon among other species  Alu element copy number differences can be used to distinguish between and construct phylogenies of primate species  Canines differ primarily in their abundance of SINEC_Cf repeats throughout the genome, rather than other gene or allele level mutations  These dog-specific SINEs may code for a splice acceptor site, altering the sequences that appear as exons or introns in each species Apart from mammals, SINEs can reach high copy numbers in a range of species, including nonbony vertebrates (elephant shark) and some fish species (coelacanths)  In plants, SINEs are often restricted to closely related species and have emerged, decayed, and vanished frequently during evolution  Nevertheless, some SINE families such as the Au-SINEs and the Angio-SINEs are unusually widespread across many often unrelated plant species 

Diseases
There are >50 human diseases associated with SINEs  When inserted near or within the exon, SINEs can cause improper splicing, become coding regions, or change the reading frame, often leading to disease phenotypes in humans and other animals  Insertion of Alu elements in the human genome is associated with breast cancer, colon cancer, leukemia, hemophilia, Dent's disease, cystic fibrosis, neurofibromatosis, and many others 

microRNAs
The role of short-interspersed nuclear elements in gene regulation within cells has been supported by multiple studies  One such study examined the correlation between a certain family of SINEs with microRNAs (in zebrafish)  The specific family of SINEs being examined was the Anamnia V-SINEs; this family of short interspersed nuclear elements is often found in the untranslated region of the 3' end of many genes and is present in vertebrate genomes   The study involved a computational analysis in which the genomic distribution and activity of the Anamnia V-SINEs in Danio rerio zebrafish was examined; furthermore, these V-SINEs potential to generate novel microRNA loci was analyzed   It was found that genes which were predicted to possess V-SINEs were targeted by microRNAs with significantly higher hybridization E-values (relative to other areas in the genome)   The genes that had high hybridization E-values were genes particularly involved in metabolic and signaling pathways  Almost all miRNAs identified to have a strong ability to hybridize to putative V-SINE sequence motifs in genes have been identified (in mammals) to have regulatory roles  These results which establish a correlation between short-interspersed nuclear elements and different regulatory microRNAs strongly suggest that V-SINEs have a significant role in attenuating responses to different signals and stimuli related to metabolism, proliferation and differentiation  Many other studies must be undertaken to establish the validity and extent of short-interspersed nuclear element retrotransposons' role in regulatory gene-expression networks  In conclusion, though not much is known about the role and mechanism by which SINEs generate miRNA gene loci  it is generally understood that SINEs have played a significant evolutionary role in the creation of "RNA-genes", this is also touched upon above in SINEs and pseudogenes 
With such evidence suggesting that short-interspersed nuclear elements have been evolutionary sources for microRNA loci generation it is important to further discuss the potential relationships between the two as well as the mechanism by which the microRNA regulates RNA degradation and more broadly, gene expression   A microRNA is a non-coding RNA generally 22 nucleotides in length  This non-protein coding oligonucleotide is itself coded by longer nuclear DNA sequence usually transcribed by RNA polymerase II which is also responsible for the transcription of most mRNAs and snRNAs in eukaryotes   However, some research suggests that some microRNAs that possess upstream short-interspersed nuclear elements are transcribed by RNA polymerase III which is widely implicated in ribosomal RNA and tRNA, two transcripts vital to mRNA translation   This provides an alternate mechanism by which short-interspersed nuclear elements could be interacting with or mediating gene-regulatory networks involving microRNAs 
The regions coding miRNA can be independent RNA-genes often being anti-sense to neighboring protein-coding genes, or can be found within the introns of protein-coding genes  The co-localization of microRNA and protein-coding genes provides a mechanistic foundation by which microRNA regulates gene-expression  Furthermore, Scarpato et al   reveals (as discussed above) that genes predicted to possess short-interspersed nuclear elements (SINEs) through sequence analysis were targeted and hybridized by microRNAs significantly greater than other genes  This provides an evolutionarily path by which the parasitic SINEs were co-opted and utilized to form RNA-genes (such as microRNAs) which have evolved to play a role in complex gene-regulatory networks 
The microRNAs are transcribed as part of longer RNA strands of generally about 80 nucleotides which through complementary base-pairing are able to form hairpin loop structures These structures are recognized and processed in the nucleus by the nuclear protein DiGeorge Syndrome Critical Region 8 (DGCR8) which recruits and associates with the Drosha protein   This complex is responsible for cleaving some of the hair-pin structures from the pre-microRNA which is transported to the cytoplasm  The pre-miRNA is processed by the protein DICER into a double stranded 22 nucleotide   Thereafter, one of the strands is incorporated into a multi-protein RNA-induced silencing complex (RISC)  Among these proteins are proteins from the Argonaute family which are critical to the complex's ability to interact with and repress the translation of the target mRNA Understanding the different ways in which microRNA regulates gene-expression, including mRNA-translation and degradation is key to understanding the potential evolutionary role of SINEs in gene-regulation and in the generation of microRNA loci  This, in addition to SINEs' direct role in regulatory networks (as discussed in SINEs as long non-coding RNAs) is crucial to beginning to understand the relationship between SINEs and certain diseases  Multiple studies have suggested that increased SINE activity is correlated with certain gene-expression profiles and post-transcription regulation of certain genes   In fact, Peterson et al  2013 demonstrated that high SINE RNA expression correlates with post-transcriptional downregulation of BRCA1, a tumor suppressor implicated in multiple forms of cancer, namely breast cancer  Furthermore, studies have established a strong correlation between transcriptional mobilization of SINEs and certain cancers and conditions such as hypoxia; this can be due to the genomic instability caused by SINE activity as well as more direct-downstream effects   SINEs have also been implicated in countless other diseases  In essence, short-interspersed nuclear elements have become deeply integrated in countless regulatory, metabolic and signaling pathways and thus play an inevitable role in causing disease  Much is still to be known about these genomic parasites but it is clear they play a significant role within eukaryotic organisms 

SINEs and pseudogenes
The activity of SINEs however has genetic vestiges which do not seem to play a significant role, positive or negative, and manifest themselves in the genome as pseudogenes   SINEs however should not be mistaken as RNA pseudogenes  In general, pseudogenes are generated when processed mRNAs of protein-coding genes are reverse-transcribed and incorporated back into the genome (RNA pseudogenes are reverse transcribed RNA genes)  Pseudogenes are generally functionless as they descend from processed RNAs independent of their evolutionary-context which includes introns and different regulatory elements which enable transcription and processing  These pseudogenes, though non-functional may in some cases still possess promoters, CpG islands, and other features which enable transcription; they thus can still be transcribed and may possess a role in the regulation of gene expression (like SINEs and other non-coding elements)   Pseudogenes thus differ from SINEs in that they are derived from transcribed- functional RNA whereas SINEs are DNA elements which retrotranspose by co-opting RNA genes transcriptional machinery  However, there are studies which suggest  that retro-transposable elements such as short-interspersed nuclear elements are not only capable of copying themselves in alternate regions in the genome but are also able to do so for random genes too   Thus SINEs can be playing a vital role in the generation of pseudogenes, which themselves are known to be involved in regulatory networks  This is perhaps another means by which SINEs have been able to influence and contribute to gene-regulation 
Passage 2:
Exonuclease VII
The enzyme exodeoxyribonuclease VII (EC 3 1 11 6, Escherichia coli exonuclease VII, E  coli exonuclease VII, endodeoxyribonuclease VII, exodeoxyribonuclease VII) is a bacterial exonuclease enzyme  It is composed of two nonidentical subunits; one large subunit and 4 small ones  that catalyses exonucleolytic cleavage in either 5′- to 3′- or 3′- to 5′-direction to yield nucleoside 5′-phosphates  The large subunit also contains an N-terminal OB-fold domain that binds to nucleic acids 
Passage 3:
Ribosomal protein
A ribosomal protein (r-protein or rProtein) is any of the proteins that, in conjunction with rRNA, make up the ribosomal subunits involved in the cellular process of translation  E  coli, other bacteria and Archaea have a 30S small subunit and a 50S large subunit, whereas humans and yeasts have a 40S small subunit and a 60S large subunit  Equivalent subunits are frequently numbered differently between bacteria, Archaea, yeasts and humans A large part of the knowledge about these organic molecules has come from the study of E  coli ribosomes  All ribosomal proteins have been isolated and many specific antibodies have been produced  These, together with electronic microscopy and the use of certain reactives, have allowed for the determination of the topography of the proteins in the ribosome   More recently, a near-complete (near)atomic picture of the ribosomal proteins is emerging from the latest high-resolution cryo-EM data (including PDB: 5AFI​) 

Conservation
Ribosomal proteins are among the most highly conserved proteins across all life forms  Among the 40 proteins found in various small ribosomal subunits (RPSs), 15 subunits are universally conserved across prokaryotes and eukaryotes  However, 7 subunits are only found in bacteria (bS21, bS6, bS16, bS18, bS20, bS21, and bTHX), while 17 subunits are only found in archaea and eukaryotes  Typically 22 proteins are found in bacterial small subunits and 32 in yeast, human and most likely most other eukaryotic species  Twenty-seven (out of 32) proteins of the eukaryotic small ribosomal subunit proteins are also present in archaea (no ribosomal protein is exclusively found in archaea), confirming that they are more closely related to eukaryotes than to bacteria Among the large ribosomal subunit (RPLs), 18 proteins are universal, i e  found in both bacteria, eukaryotes, and archaea  14 proteins are only found in bacteria, while 27 proteins are only found in archaea and eukaryotes  Again, archaea have no proteins unique to them 

Essentiality
Despite their high conservation over billions of years of evolution, the absence of several ribosomal proteins in certain species shows that ribosomal subunits have been added and lost over the course of evolution  This is also reflected by the fact that several ribosomal proteins do not appear to be essential when deleted  For instance, in E  coli nine ribosomal proteins (uL15, bL21, uL24, bL27, uL29, uL30, bL34, uS9, and uS17) are nonessential for survival when deleted  Taken together with previous results, 22 of the 54 E  coli ribosomal protein genes can be individually deleted from the genome  Similarly, 16 ribosomal proteins (uL1, bL9, uL15, uL22, uL23, bL28, uL29, bL32, bL33 1, bL33 2, bL34, bL35, bL36, bS6, bS20, and bS21) were successfully deleted in Bacillus subtilis  In conjunction with previous reports, 22 ribosomal proteins have been shown to be nonessential in B  subtilis, at least for cell proliferation 

Assembly
In E  coli
The ribosome of E  coli has about 22 proteins in the small subunit (labelled S1 to S22) and 33 proteins in the large subunit (somewhat counter-intuitively called L1 to L36)  All of them are different with three exceptions: one protein is found in both subunits (S20 and L26), L7 and L12 are acetylated and methylated forms of the same protein, and L8 is a complex of L7/L12 and L10  In addition, L31 is known to exist in two forms, the full length at 7 9 kilodaltons (kDa) and fragmented at 7 0 kDa  This is why the number of proteins in a ribosome is of 56  Except for S1 (with a molecular weight of 61 2 kDa), the other proteins range in weight between 4 4 and 29 7 kDa Recent de novo proteomics experiments where the authors characterized in vivo ribosome-assembly intermediates and associated assembly factors from wild-type Escherichia coli cells using a general quantitative mass spectrometry (qMS) approach have confirmed the presence of all the known small and large subunit components and have identified a total of 21 known and potentially new ribosome-assembly-factors that co-localise with various ribosomal particles 

Disposition in the small ribosomal subunit
In the small (30S) subunit of E  coli ribosomes, the proteins denoted uS4, uS7, uS8, uS15, uS17, bS20 bind independently to 16S rRNA  After assembly of these primary binding proteins, uS5, bS6, uS9, uS12, uS13, bS16, bS18, and uS19 bind to the growing ribosome  These proteins also potentiate the addition of uS2, uS3, uS10, uS11, uS14, and bS21  Protein binding to helical junctions is important for initiating the correct tertiary fold of RNA and to organize the overall structure  Nearly all the proteins contain one or more globular domains  Moreover, nearly all contain long extensions that can contact the RNA in far-reaching regions  Additional stabilization results from the proteins' basic residues, as these neutralize the charge repulsion of the RNA backbone  Protein–protein interactions also exist to hold structure together by electrostatic and hydrogen bonding interactions  Theoretical investigations pointed to correlated effects of protein-binding onto binding affinities during the assembly processIn one study, the net charges (at pH 7 4) of the ribosomal proteins comprising the highly conserved S10-spc cluster were found to have an inverse relationship with the halophilicity/halotolerance levels in bacteria and archaea  In non-halophilic bacteria, the S10-spc proteins are generally basic, contrasting with the overall acidic whole proteomes of the extremely halophiles  The universal uL2 lying in the oldest part of the ribosome, is always positively charged irrespective of the strain/organism it belongs to 

In eukaryotes
Ribosomes in eukaryotes contain 79–80 proteins and four ribosomal RNA (rRNA) molecules 
General or specialized chaperones solubilize the ribosomal proteins and facilitate their import into the nucleus  Assembly of the eukaryotic ribosome appears to be driven by the ribosomal proteins in vivo when assembly is also aided by chaperones   Most ribosomal proteins assemble with rRNA co-transcriptionally, becoming associated more stably as assembly proceeds, and the active sites of both subunits are constructed last 

Table of ribosomal proteins
In the past, different nomenclatures were used for the same ribosomal protein in different organisms  Not only were the names not consistent across domains; the names also differed between organisms within a domain, such as humans and S  cervisiae, both eukaryotes  This was due to researchers assigning names before the sequences were known, causing trouble for later research  The following tables use the unified nomenclature by Ban et al , 2014  The same nomenclature is used by UniProt's "family" curation In general, cellular ribosomal proteins are to be called simply using the cross domain name, e g  "uL14" for what is currently called L23 in humans  A suffix is used for the organellar versions, so that "uL14m" refers to the human mitochondrial uL14 (MRPL14)  Organelle-specific proteins use their own cross-domain prefixes, for example "mS33" for MRPS33: Table S3,S4  and "cL37" for PSRP5 : Table S2,S3  (See the two proceeding citations, also partially by Ban N, for the organelle nomenclatures )

See also
Alpha operon ribosome binding site
Ribosomal protein L20 leader
Mitochondrial ribosome, for a list of its protein subunits
Passage 4:
Nuclear cap-binding protein complex
Nuclear cap-binding protein complex is a RNA-binding protein which binds to the 5' cap of pre-mRNA  The cap and nuclear cap-binding protein have many functions in mRNA biogenesis including splicing, 3'-end formation by stabilizing the interaction of the 3'-end processing machinery, nuclear export and protection of the transcripts from nuclease degradation  When RNA is exported to the cytoplasm the nuclear cap-binding protein complex is replaced by cytoplasmic cap binding complex  The nuclear cap-binding complex is a functional heterodimer and composed of Cbc1/Cbc2 in yeast and CBC20/CBC80 in multicellular eukaryotes  Human nuclear cap-binding protein complex shows the large subunit, CBC80 consists of 757 amino acid residues  Its secondary structure contains approximately sixty percent of helical and one percent of beta sheet in the strand  The small subunit, CBC20 has 98 amino acid residues  Its secondary structure contains approximately twenty percent of helical and twenty-four percent of beta sheet in the strand  Human nuclear cap-binding protein complex plays important role in the maturation of pre-mRNA and in uracil-rich small nuclear RNA 
Passage 5:
Ribozyme
Ribozymes (ribonucleic acid enzymes) are RNA molecules that have the ability to catalyze specific biochemical reactions, including RNA splicing in gene expression, similar to the action of protein enzymes  The 1982 discovery of ribozymes demonstrated that RNA can be both genetic material (like DNA) and a biological catalyst (like protein enzymes), and contributed to the RNA world hypothesis, which suggests that RNA may have been important in the evolution of prebiotic self-replicating systems The most common activities of natural or in vitro evolved ribozymes are the cleavage (or ligation) of RNA and DNA and peptide bond formation  For example, the smallest ribozyme known (GUGGC-3') can aminoacylate a GCCU-3' sequence in the presence of PheAMP  Within the ribosome, ribozymes function as part of the large subunit ribosomal RNA to link amino acids during protein synthesis  They also participate in a variety of RNA processing reactions, including RNA splicing, viral replication, and transfer RNA biosynthesis  Examples of ribozymes include the hammerhead ribozyme, the VS ribozyme, leadzyme, and the hairpin ribozyme 
Researchers who are investigating the origins of life through the RNA world hypothesis have been working on discovering a ribozyme with the capacity to self-replicate, which would require it to have the ability to catalytically synthesize polymers of RNA  This should be able to happen in prebiotically plausible conditions with high rates of copying accuracy to prevent degradation of information but also allowing for the occurrence of occasional errors during the copying process to allow for Darwinian evolution to proceed Attempts have been made to develop ribozymes as therapeutic agents, as enzymes which target defined RNA sequences for cleavage, as biosensors, and for applications in functional genomics and gene discovery 

Discovery
Before the discovery of ribozymes, enzymes—which are defined as catalytic proteins—were the only known biological catalysts  In 1967, Carl Woese, Francis Crick, and Leslie Orgel were the first to suggest that RNA could act as a catalyst  This idea was based upon the discovery that RNA can form complex secondary structures  These ribozymes were found in the intron of an RNA transcript, which removed itself from the transcript, as well as in the RNA component of the RNase P complex, which is involved in the maturation of pre-tRNAs  In 1989, Thomas R  Cech and Sidney Altman shared the Nobel Prize in chemistry for their "discovery of catalytic properties of RNA"  The term ribozyme was first introduced by Kelly Kruger et al  in a paper published in Cell in 1982 It had been a firmly established belief in biology that catalysis was reserved for proteins  However, the idea of RNA catalysis is motivated in part by the old question regarding the origin of life: Which comes first, enzymes that do the work of the cell or nucleic acids that carry the information required to produce the enzymes? The concept of "ribonucleic acids as catalysts" circumvents this problem  RNA, in essence, can be both the chicken and the egg In the 1980s, Thomas Cech, at the University of Colorado Boulder, was studying the excision of introns in a ribosomal RNA gene in Tetrahymena thermophila  While trying to purify the enzyme responsible for the splicing reaction, he found that the intron could be spliced out in the absence of any added cell extract  As much as they tried, Cech and his colleagues could not identify any protein associated with the splicing reaction  After much work, Cech proposed that the intron sequence portion of the RNA could break and reform phosphodiester bonds  At about the same time, Sidney Altman, a professor at Yale University, was studying the way tRNA molecules are processed in the cell when he and his colleagues isolated an enzyme called RNase-P, which is responsible for conversion of a precursor tRNA into the active tRNA  Much to their surprise, they found that RNase-P contained RNA in addition to protein and that RNA was an essential component of the active enzyme  This was such a foreign idea that they had difficulty publishing their findings  The following year, Altman demonstrated that RNA can act as a catalyst by showing that the RNase-P RNA subunit could catalyze the cleavage of precursor tRNA into active tRNA in the absence of any protein component 
Since Cech's and Altman's discovery, other investigators have discovered other examples of self-cleaving RNA or catalytic RNA molecules  Many ribozymes have either a hairpin – or hammerhead – shaped active center and a unique secondary structure that allows them to cleave other RNA molecules at specific sequences  It is now possible to make ribozymes that will specifically cleave any RNA molecule  These RNA catalysts may have pharmaceutical applications  For example, a ribozyme has been designed to cleave the RNA of HIV  If such a ribozyme were made by a cell, all incoming virus particles would have their RNA genome cleaved by the ribozyme, which would prevent infection 

Structure and mechanism
Despite having only four choices for each monomer unit (nucleotides), compared to 20 amino acid side chains found in proteins, ribozymes have diverse structures and mechanisms  In many cases they are able to mimic the mechanism used by their protein counterparts  For example, in self cleaving ribozyme RNAs, an in-line SN2 reaction is carried out using the 2’ hydroxyl group as a nucleophile attacking the bridging phosphate and causing 5’ oxygen of the N+1 base to act as a leaving group  In comparison, RNase A, a protein that catalyzes the same reaction, uses a coordinating histidine and lysine to act as a base to attack the phosphate backbone Like many protein enzymes, metal binding is also critical to the function of many ribozymes  Often these interactions use both the phosphate backbone and the base of the nucleotide, causing drastic conformational changes  There are two mechanism classes for the cleavage of a phosphodiester backbone in the presence of metal  In the first mechanism, the internal 2’- OH group attacks the phosphorus center in a SN2 mechanism  Metal ions promote this reaction by first coordinating the phosphate oxygen and later stabling the oxyanion  The second mechanism also follows a SN2 displacement, but the nucleophile comes from water or exogenous hydroxyl groups rather than RNA itself  The smallest ribozyme is UUU, which can promote the cleavage between G and A of the GAAA tetranucleotide via the first mechanism in the presence of Mn2+  The reason why this trinucleotide (rather than the complementary tetramer) catalyzes this reaction may be because the UUU-AAA pairing is the weakest and most flexible trinucleotide among the 64 conformations, which provides the binding site for Mn2+ Phosphoryl transfer can also be catalyzed without metal ions  For example, pancreatic ribonuclease A and hepatitis delta virus (HDV) ribozymes can catalyze the cleavage of RNA backbone through acid-base catalysis without metal ions  Hairpin ribozyme can also catalyze the self-cleavage of RNA without metal ions, but the mechanism for this is still unclear Ribozyme can also catalyze the formation of peptide bond between adjacent amino acids by lowering the activation entropy 

Activities
Although ribozymes are quite rare in most cells, their roles are sometimes essential to life  For example, the functional part of the ribosome, the biological machine that translates RNA into proteins, is fundamentally a ribozyme, composed of RNA tertiary structural motifs that are often coordinated to metal ions such as Mg2+ as cofactors  In a model system, there is no requirement for divalent cations in a five-nucleotide RNA catalyzing trans-phenylalanation of a four-nucleotide substrate with 3 base pairs complementary with the catalyst, where the catalyst/substrate were devised by truncation of the C3 ribozyme The best-studied ribozymes are probably those that cut themselves or other RNAs, as in the original discovery by Cech and Altman  However, ribozymes can be designed to catalyze a range of reactions, many of which may occur in life but have not been discovered in cells RNA may catalyze folding of the pathological protein conformation of a prion in a manner similar to that of a chaperonin 

Ribozymes and the origin of life
RNA can also act as a hereditary molecule, which encouraged Walter Gilbert to propose that in the distant past, the cell used RNA as both the genetic material and the structural and catalytic molecule rather than dividing these functions between DNA and protein as they are today; this hypothesis is known as the "RNA world hypothesis" of the origin of life  Since nucleotides and RNA (and thus ribozymes) can arise by inorganic chemicals, they are candidates for the first enzymes, and in fact, the first "replicators" (i e , information-containing macro-molecules that replicate themselves)  An example of a self-replicating ribozyme that ligates two substrates to generate an exact copy of itself was described in 2002 
The discovery of the catalytic activity of RNA solved the "chicken and egg" paradox of the origin of life, solving the problem of origin of peptide and nucleic acid central dogma  According to this scenario, at the origin of life, all enzymatic activity and genetic information encoding was done by one molecule: RNA 
Ribozymes have been produced in the laboratory that are capable of catalyzing the synthesis of other RNA molecules from activated monomers under very specific conditions, these molecules being known as RNA polymerase ribozymes  The first RNA polymerase ribozyme was reported in 1996, and was capable of synthesizing RNA polymers up to 6 nucleotides in length  Mutagenesis and selection has been performed on an RNA ligase ribozyme from a large pool of random RNA sequences, resulting in isolation of the improved "Round-18" polymerase ribozyme in 2001 which could catalyze RNA polymers now up to 14 nucleotides in length  Upon application of further selection on the Round-18 ribozyme, the B6 61 ribozyme was generated and was able to add up to 20 nucleotides to a primer template in 24 hours, until it decomposes by cleavage of its phosphodiester bonds The rate at which ribozymes can polymerize an RNA sequence multiples substantially when it takes place within a micelle The next ribozyme discovered was the "tC19Z" ribozyme, which can add up to 95 nucleotides with a fidelity of 0 0083 mutations/nucleotide  Next, the "tC9Y" ribozyme was discovered by researchers and was further able to synthesize RNA strands up to 206 nucleotides long in the eutectic phase conditions at below-zero temperature, conditions previously shown to promote ribozyme polymerase activity The RNA polymerase ribozyme (RPR) called tC9-4M was able to polymerize RNA chains longer than itself (i e  longer than 177 nt) in magnesium ion concentrations close to physiological levels, whereas earlier RPRs required prebiotically implausible concentrations of up to 200 mM  The only factor required for it to achieve this was the presence of a very simple amino acid polymer called lysine decapeptide The most complex RPR synthesized by that point was called 24-3, which was newly capable of polymerizing the sequences of a substantial variety of nucleotide sequences and navigating through complex secondary structures of RNA substrates inaccessible to previous ribozymes  In fact, this experiment was the first to use a ribozyme to synthesize a tRNA molecule  Starting with the 24-3 ribozyme, Tjhung et al  applied another fourteen rounds of selection to obtain an RNA polymerase ribozyme by in vitro evolution termed '38-6' that has an unprecedented level of activity in copying complex RNA molecules  However, this ribozyme is unable to copy itself and its RNA products have a high mutation rate  In a subsequent study, the researchers began with the 38-6 ribozyme and applied another 14 rounds of selection to generate the '52-2' ribozyme, which compared to 38-6, was again many times more active and could begin generating detectable and functional levels of the class I ligase, although it was still limited in its fidelity and functionality in comparison to copying of the same template by proteins such as the T7 RNA polymerase An RPR called t5(+1) adds triplet nucleotides at a time instead of just one nucleotide at a time  This heterodimeric RPR can navigate secondary structures inaccessible to 24-3, including hairpins  In the initial pool of RNA variants derived only from a previously synthesized RPR known as the Z RPR, two sequences separately emerged and evolved to be mutualistically dependent on each other  The Type 1 RNA evolved to be catalytically inactive, but complexing with the Type 5 RNA boosted its polymerization ability and enabled intermolecular interactions with the RNA template substrate obviating the need to tether the template directly to the RNA sequence of the RPR, which was a limitation of earlier studies  Not only did t5(+1) not need tethering to the template, but a primer was not needed either as t5(+1) had the ability to polymerize a template in both 3' → 5' and 5' 3 → 3' directions A highly evolved RNA polymerase ribozyme was able to function as a reverse transcriptase, that is, it can synthesize a DNA copy using an RNA template  Such an activity is considered to have been crucial for the transition from RNA to DNA genomes during the early history of life on earth  Reverse transcription capability could have arisen as a secondary function of an early RNA-dependent RNA polymerase ribozyme 
An RNA sequence that folds into a ribozyme is capable of invading duplexed RNA, rearranging into an open holopolymerase complex, and then searching for a specific RNA promoter sequence, and upon recognition rearrange again into a processive form that polymerizes a complementary strand of the sequence  This ribozyme is capable of extending duplexed RNA by up to 107 nucleotides, and does so without needing to tether the sequence being polymerized 

Artificial ribozymes
Since the discovery of ribozymes that exist in living organisms, there has been interest in the study of new synthetic ribozymes made in the laboratory  For example, artificially produced self-cleaving RNAs with good enzymatic activity have been produced  Tang and Breaker isolated self-cleaving RNAs by in vitro selection of RNAs originating from random-sequence RNAs  Some of the synthetic ribozymes that were produced had novel structures, while some were similar to the naturally occurring hammerhead ribozyme 
In 2015, researchers at Northwestern University and the University of Illinois Chicago engineered a tethered ribosome that works nearly as well as the authentic cellular component that produces all the proteins and enzymes within the cell  Called Ribosome-T, or Ribo-T, the artificial ribosome was created by Michael Jewett and Alexander Mankin  The techniques used to create artificial ribozymes involve directed evolution  This approach takes advantage of RNA's dual nature as both a catalyst and an informational polymer, making it easy for an investigator to produce vast populations of RNA catalysts using polymerase enzymes  The ribozymes are mutated by reverse transcribing them with reverse transcriptase into various cDNA and amplified with error-prone PCR  The selection parameters in these experiments often differ  One approach for selecting a ligase ribozyme involves using biotin tags, which are covalently linked to the substrate  If a molecule possesses the desired ligase activity, a streptavidin matrix can be used to recover the active molecules 
Lincoln and Joyce used in vitro evolution to develop ribozyme ligases capable of self-replication in about an hour, via the joining of pre-synthesized highly complementary oligonucleotides Although not true catalysts, the creation of artificial self-cleaving riboswitches, termed aptazymes, has also been an active area of research  Riboswitches are regulatory RNA motifs that change their structure in response to a small molecule ligand to regulate translation  While there are many known natural riboswitches that bind a wide array of metabolites and other small organic molecules, only one ribozyme based on a riboswitch has been described: glmS  Early work in characterizing self-cleaving riboswitches was focused on using theophylline as the ligand  In these studies, an RNA hairpin is formed which blocks the ribosome binding site, thus inhibiting translation  In the presence of the ligand, in these cases theophylline, the regulatory RNA region is cleaved off, allowing the ribosome to bind and translate the target gene  Much of this RNA engineering work was based on rational design and previously determined RNA structures rather than directed evolution as in the above examples  More recent work has broadened the ligands used in ribozyme riboswitches to include thymine pyrophosphate  Fluorescence-activated cell sorting has also been used to engineering aptazymes 

Applications
Ribozymes have been proposed and developed for the treatment of disease through gene therapy  One major challenge of using RNA-based enzymes as a therapeutic is the short half-life of the catalytic RNA molecules in the body  To combat this, the 2’ position on the ribose is modified to improve RNA stability  One area of ribozyme gene therapy has been the inhibition of RNA-based viruses 
A type of synthetic ribozyme directed against HIV RNA called gene shears has been developed and has entered clinical testing for HIV infection Similarly, ribozymes have been designed to target the hepatitis C virus RNA, SARS coronavirus (SARS-CoV), Adenovirus and influenza A and B virus RNA  The ribozyme is able to cleave the conserved regions of the virus's genome, which has been shown to reduce the virus in mammalian cell culture  Despite these efforts by researchers, these projects have remained in the preclinical stage 

Known ribozymes
Well-validated naturally occurring ribozyme classes:

See also
Notes and references
Further reading
External links
Tom Cech's Short Talk: "Discovering Ribozymes"
Passage 6:
Ribosome
Ribosomes () are macromolecular machines, found within all  cells, that perform biological protein synthesis (mRNA translation)  Ribosomes link amino acids together in the order specified by the codons of messenger RNA (mRNA) molecules to form polypeptide chains  Ribosomes consist of two major components: the small and large ribosomal subunits  Each subunit consists of one or more ribosomal RNA (rRNA) molecules and many ribosomal proteins (RPs or r-proteins)  The ribosomes and associated molecules are also known as the translational apparatus 

Overview
The sequence of DNA that encodes the sequence of the amino acids in a protein is transcribed into a messenger RNA chain  Ribosomes bind to messenger RNAs and use their sequences for determining the correct sequence of amino acids to generate a given protein  Amino acids are selected and carried to the ribosome by transfer RNA (tRNA) molecules, which enter the ribosome and bind to the messenger RNA chain via an anti-codon stem loop  For each coding triplet (codon) in the messenger RNA, there is a unique transfer RNA that must have the exact anti-codon match, and carries the correct amino acid for incorporating into a growing polypeptide chain  Once the protein is produced, it can then fold to produce a functional three-dimensional structure 
A ribosome is made from complexes of RNAs and proteins and is therefore a ribonucleoprotein complex  Each ribosome is composed of small (30S) and large (50S) components, called subunits, which are bound to each other:

(30S) has mainly a decoding function and is also bound to the mRNA
(50S) has mainly a catalytic function and is also bound to the aminoacylated tRNAs The synthesis of proteins from their building blocks takes place in four phases: initiation, elongation, termination, and recycling  The start codon in all mRNA molecules has the sequence AUG  The stop codon is one of UAA, UAG, or UGA; since there are no tRNA molecules that recognize these codons, the ribosome recognizes that translation is complete  When a ribosome finishes reading an mRNA molecule, the two subunits separate and are usually broken up but can be re-used  Ribosomes are ribozymes, because the catalytic peptidyl transferase activity that links amino acids together is performed by the ribosomal RNA Ribosomes are often associated with the intracellular membranes that make up the rough endoplasmic reticulum 
Ribosomes from bacteria, archaea and eukaryotes in the three-domain system resemble each other to a remarkable degree, evidence of a common origin  They differ in their size, sequence, structure, and the ratio of protein to RNA  The differences in structure allow some antibiotics to kill bacteria by inhibiting their ribosomes, while leaving human ribosomes unaffected  In all species, more than one ribosome may move along a single mRNA chain at one time (as a polysome), each "reading" a specific sequence and producing a corresponding protein molecule 
The mitochondrial ribosomes of eukaryotic cells functionally resemble many features of those in bacteria, reflecting the likely evolutionary origin of mitochondria 

Discovery
Ribosomes were first observed in the mid-1950s by Romanian-American cell biologist George Emil Palade, using an electron microscope, as dense particles or granules   They were initially called  Palade granules due to their granular structure   The term "ribosome" was proposed in 1958:

During the course of the symposium a semantic difficulty became apparent  To some of the participants, "microsomes" mean the ribonucleoprotein particles of the microsome fraction contaminated by other protein and lipid material; to others, the microsomes consist of protein and lipid contaminated by particles  The phrase "microsomal particles" does not seem adequate, and "ribonucleoprotein particles of the microsome fraction" is much too awkward  During the meeting, the word "ribosome" was suggested, which has a very satisfactory name and a pleasant sound  The present confusion would be eliminated if "ribosome" were adopted to designate ribonucleoprotein particles in sizes ranging from 35 to 100S 
Albert Claude, Christian de Duve, and George Emil Palade were jointly awarded the Nobel Prize in Physiology or Medicine, in 1974, for the discovery of the ribosome  The Nobel Prize in Chemistry 2009 was awarded to Venkatraman Ramakrishnan, Thomas A  Steitz and Ada E  Yonath for determining the detailed structure and mechanism of the ribosome 

Structure
The ribosome is a complex cellular machine  It is largely made up of specialized RNA known as ribosomal RNA (rRNA) as well as dozens of distinct proteins (the exact number varies slightly between species)  The ribosomal proteins and rRNAs are arranged into two distinct ribosomal pieces of different sizes, known generally as the large and small subunit of the ribosome  Ribosomes consist of two subunits that fit together (Figure 2) and work as one to translate the mRNA into a polypeptide chain during protein synthesis (Figure 1)  Because they are formed from two subunits of non-equal size, they are slightly longer in the axis than in diameter 

Prokaryotic ribosomes
Prokaryotic ribosomes are around 20 nm (200 Å) in diameter and are composed of 65% rRNA and 35% ribosomal proteins  Eukaryotic ribosomes are between 25 and 30 nm (250–300 Å) in diameter with an rRNA-to-protein ratio that is close to 1  Crystallographic work has shown that there are no ribosomal proteins close to the reaction site for polypeptide synthesis  This suggests that the protein components of ribosomes do not directly participate in peptide bond formation catalysis, but rather that these proteins act as a scaffold that may enhance the ability of rRNA to synthesize protein (See: Ribozyme) 

The ribosomal subunits of prokaryotes and eukaryotes are quite similar The unit of measurement used to describe the ribosomal subunits and the rRNA fragments is the Svedberg unit, a measure of the rate of sedimentation in centrifugation rather than size  This accounts for why fragment names do not add up: for example, bacterial 70S ribosomes are made of 50S and 30S subunits 
Prokaryotes have 70S ribosomes, each consisting of a small (30S) and a large (50S) subunit  E  coli, for example, has a 16S RNA subunit (consisting of 1540 nucleotides) that is bound to 21 proteins  The large subunit is composed of a 5S RNA subunit (120 nucleotides), a 23S RNA subunit (2900 nucleotides) and 31 proteins 
Affinity label for the tRNA binding sites on the E  coli ribosome allowed the identification of A and P site proteins most likely associated with the peptidyltransferase activity; labelled proteins are L27, L14, L15, L16, L2; at least L27 is located at the donor site, as shown by E  Collatz and A P  Czernilofsky  Additional research has demonstrated that the S1 and S21 proteins, in association with the 3′-end of 16S ribosomal RNA, are involved in the initiation of translation 

Archaeal ribosomes
Archaeal ribosomes share the same general dimensions of bacteria ones, being a 70S ribosome made up from a 50S large subunit, a 30S small subunit, and containing three rRNA chains  However, on the sequence level, they are much closer to eukaryotic ones than to bacterial ones  Every extra ribosomal protein archaea have compared to bacteria has a eukaryotic counterpart, while no such relation applies between archaea and bacteria 

Eukaryotic ribosomes
Eukaryotes have 80S ribosomes located in their cytosol, each consisting of a small (40S) and large (60S) subunit  Their 40S subunit has an 18S RNA (1900 nucleotides) and 33 proteins  The large subunit is composed of a 5S RNA (120 nucleotides), 28S RNA (4700 nucleotides), a 5 8S RNA (160 nucleotides) subunits and 49 proteins 
During 1977, Czernilofsky published research that used affinity labeling to identify tRNA-binding sites on rat liver ribosomes  Several proteins, including L32/33, L36, L21, L23, L28/29 and L13 were implicated as being at or near the peptidyl transferase center 

Plastoribosomes and mitoribosomes
In eukaryotes, ribosomes are present in mitochondria (sometimes called mitoribosomes) and in plastids such as chloroplasts (also called plastoribosomes)  They also consist of large and small subunits bound together with proteins into one 70S particle  These ribosomes are similar to those of bacteria and these organelles are thought to have originated as symbiotic bacteria  Of the two, chloroplastic ribosomes are closer to bacterial ones than mitochondrial ones are  Many pieces of ribosomal RNA in the mitochondria are shortened, and in the case of 5S rRNA, replaced by other structures in animals and fungi  In particular, Leishmania tarentolae has a minimalized set of mitochondrial rRNA  In contrast, plant mitoribosomes have both extended rRNA and additional proteins as compared to bacteria, in particular, many pentatricopetide repeat proteins The cryptomonad and chlorarachniophyte algae may contain a nucleomorph that resembles a vestigial eukaryotic nucleus  Eukaryotic 80S ribosomes may be present in the compartment containing the nucleomorph 

Making use of the differences
The differences between the bacterial and eukaryotic ribosomes are exploited by pharmaceutical chemists to create antibiotics that can destroy a bacterial infection without harming the cells of the infected person  Due to the differences in their structures, the bacterial 70S ribosomes are vulnerable to these antibiotics while the eukaryotic 80S ribosomes are not  Even though mitochondria possess ribosomes similar to the bacterial ones, mitochondria are not affected by these antibiotics because they are surrounded by a double membrane that does not easily admit these antibiotics into the organelle  A noteworthy counterexample, however, includes the antineoplastic antibiotic chloramphenicol, which successfully inhibits bacterial 50S and eukaryotic mitochondrial 50S ribosomes  Ribosomes in chloroplasts, however, are different: Antibiotic resistance in chloroplast ribosomal proteins is a trait that has to be introduced as a marker, with genetic engineering 

Common properties
The various ribosomes share a core structure, which is quite similar despite the large differences in size  Much of the RNA is highly organized into various tertiary structural motifs, for example pseudoknots that exhibit coaxial stacking  The extra RNA in the larger ribosomes is in several long continuous insertions, such that they form loops out of the core structure without disrupting or changing it  All of the catalytic activity of the ribosome is carried out by the RNA; the proteins reside on the surface and seem to stabilize the structure 

High-resolution structure
The general molecular structure of the ribosome has been known since the early 1970s  In the early 2000s, the structure has been achieved at high resolutions, of the order of a few ångströms 
The first papers giving the structure of the ribosome at atomic resolution were published almost simultaneously in late 2000  The 50S (large prokaryotic) subunit was determined from the archaeon Haloarcula marismortui and the bacterium Deinococcus radiodurans, and the structure of the 30S subunit was determined from Thermus thermophilus  These structural studies were awarded the Nobel Prize in Chemistry in 2009  In May 2001 these coordinates were used to reconstruct the entire T  thermophilus 70S particle at 5 5 Å resolution Two papers were published in November 2005 with structures of the Escherichia coli 70S ribosome  The structures of a vacant ribosome were determined at 3 5 Å resolution using X-ray crystallography  Then, two weeks later, a structure based on cryo-electron microscopy was published, which depicts the ribosome at 11–15 Å resolution in the act of passing a newly synthesized protein strand into the protein-conducting channel 
The first atomic structures of the ribosome complexed with tRNA and mRNA molecules were solved by using X-ray crystallography by two groups independently, at 2 8 Å and at 3 7 Å  These structures allow one to see the details of interactions of the Thermus thermophilus ribosome with mRNA and with tRNAs bound at classical ribosomal sites  Interactions of the ribosome with long mRNAs containing Shine-Dalgarno sequences were visualized soon after that at 4 5–5 5 Å resolution In 2011, the first complete atomic structure of the eukaryotic 80S ribosome from the yeast Saccharomyces cerevisiae   was obtained by crystallography  The model reveals the architecture of eukaryote-specific elements and their interaction with the universally conserved core  At the same time, the complete model of a eukaryotic 40S ribosomal structure in Tetrahymena thermophila was published and described the structure of the 40S subunit, as well as much about the 40S subunit's interaction with eIF1 during translation initiation  Similarly, the eukaryotic 60S subunit structure was also determined from Tetrahymena thermophila in complex with eIF6 

Function
Ribosomes are minute particles consisting of RNA and associated proteins that function to synthesize proteins  Proteins are needed for many cellular functions such as repairing damage or directing chemical processes  Ribosomes can be found floating within the cytoplasm or attached to the endoplasmic reticulum  Their main function is to convert genetic code into an amino acid sequence and to build protein polymers from amino acid monomers 
Ribosomes act as catalysts in two extremely important biological processes called peptidyl transfer and peptidyl hydrolysis  The "PT center is responsible for producing protein bonds during protein elongation" In summary, ribosomes have two main functions: Decoding the message, and the formation of peptide bonds  These two functions reside in the ribosomal subunits  Each subunit is made of one or more rRNAs and many r-proteins  The small subunit (30S in bacteria and archaea, 40S in eukaryotes) has the decoding function, whereas the large subunit (50S in bacteria and archaea, 60S in eukaryotes) catalyzes the formation of peptide bonds, referred to as the peptidyl-transferase activity  The bacterial (and archaeal) small subunit contains the 16S rRNA and 21 r-proteins (Escherichia coli), whereas the eukaryotic small subunit contains the 18S rRNA and 32 r-proteins (Saccharomyces cerevisiae; although the numbers vary between species)  The bacterial large subunit contains the 5S and 23S rRNAs and 34 r-proteins (E  coli), with the eukaryotic large subunit containing the 5S, 5 8S, and 25S / 28S rRNAs and 46 r-proteins (S  cerevisiae; again, the exact numbers vary between species) 

Translation
Ribosomes are the workplaces of protein biosynthesis, the process of translating mRNA into protein  The mRNA comprises a series of codons which are decoded by the ribosome so as to make the protein  Using the mRNA as a template, the ribosome traverses each codon (3 nucleotides) of the mRNA, pairing it with the appropriate amino acid provided by an aminoacyl-tRNA  Aminoacyl-tRNA contains a complementary anticodon on one end and the appropriate amino acid on the other  For fast and accurate recognition of the appropriate tRNA, the ribosome utilizes large conformational changes (conformational proofreading)  The small ribosomal subunit, typically bound to an aminoacyl-tRNA containing the first amino acid methionine, binds to an AUG codon on the mRNA and recruits the large ribosomal subunit  The ribosome contains three RNA binding sites, designated A, P, and E  The A-site binds an aminoacyl-tRNA or termination release factors; the P-site binds a peptidyl-tRNA (a tRNA bound to the poly-peptide chain); and the E-site (exit) binds a free tRNA  Protein synthesis begins at a start codon AUG near the 5' end of the mRNA  mRNA binds to the P site of the ribosome first  The ribosome recognizes the start codon by using the Shine-Dalgarno sequence of the mRNA in prokaryotes and Kozak box in eukaryotes 
Although catalysis of the peptide bond involves the C2 hydroxyl of RNA's P-site adenosine in a proton shuttle mechanism, other steps in protein synthesis (such as translocation) are caused by changes in protein conformations  Since their catalytic core is made of RNA, ribosomes are classified as "ribozymes," and it is thought that they might be remnants of the RNA world 
In Figure 5, both ribosomal subunits (small and large) assemble at the start codon (towards the 5' end of the mRNA)  The ribosome uses tRNA that matches the current codon (triplet) on the mRNA to append an amino acid to the polypeptide chain  This is done for each triplet on the mRNA, while the ribosome moves towards the 3' end of the mRNA  Usually in bacterial cells, several ribosomes are working parallel on a single mRNA, forming what is called a polyribosome or polysome 

Cotranslational folding
The ribosome is known to actively participate in the protein folding  The structures obtained in this way are usually identical to the ones obtained during protein chemical refolding; however, the pathways leading to the final product may be different  In some cases, the ribosome is crucial in obtaining the functional protein form  For example, one of the possible mechanisms of folding of the deeply knotted proteins relies on the ribosome pushing the chain through the attached loop 

Addition of translation-independent amino acids
Presence of a ribosome quality control protein Rqc2 is associated with mRNA-independent protein elongation  This elongation is a result of ribosomal addition (via tRNAs brought by Rqc2) of CAT tails: ribosomes extend the C-terminus of a stalled protein with random, translation-independent sequences of alanines and threonines 

Ribosome locations
Ribosomes are classified as being either "free" or "membrane-bound" 

Free and membrane-bound ribosomes differ only in their spatial distribution; they are identical in structure  Whether the ribosome exists in a free or membrane-bound state depends on the presence of an ER-targeting signal sequence on the protein being synthesized, so an individual ribosome might be membrane-bound when it is making one protein, but free in the cytosol when it makes another protein 
Ribosomes are sometimes referred to as organelles, but the use of the term organelle is often restricted to describing sub-cellular components that include a phospholipid membrane, which ribosomes, being entirely particulate, do not  For this reason, ribosomes may sometimes be described as "non-membranous organelles" 

Free ribosomes
Free ribosomes can move about anywhere in the cytosol, but are excluded from the cell nucleus and other organelles  Proteins that are formed from free ribosomes are released into the cytosol and used within the cell  Since the cytosol contains high concentrations of glutathione and is, therefore, a reducing environment, proteins containing disulfide bonds, which are formed from oxidized cysteine residues, cannot be produced within it 

Membrane-bound ribosomes
When a ribosome begins to synthesize proteins that are needed in some organelles, the ribosome making this protein can become "membrane-bound"  In eukaryotic cells this happens in a region of the endoplasmic reticulum (ER) called the "rough ER"  The newly produced polypeptide chains are inserted directly into the ER by the ribosome undertaking vectorial synthesis and are then transported to their destinations, through the secretory pathway  Bound ribosomes usually produce proteins that are used within the plasma membrane or are expelled from the cell via exocytosis 

Biogenesis
In bacterial cells, ribosomes are synthesized in the cytoplasm through the transcription of multiple ribosome gene operons  In eukaryotes, the process takes place both in the cell cytoplasm and in the nucleolus, which is a region within the cell nucleus  The assembly process involves the coordinated function of over 200 proteins in the synthesis and processing of the four rRNAs, as well as assembly of those rRNAs with the ribosomal proteins 

Origin
The ribosome may have first originated as a protoribosome, possibly containing a peptidyl transferase centre (PTC), in an RNA world, appearing as a self-replicating complex that only later evolved the ability to synthesize proteins when amino acids began to appear  Studies suggest that ancient ribosomes constructed solely of rRNA could have developed the ability to synthesize peptide bonds  In addition, evidence strongly points to ancient ribosomes as self-replicating complexes, where the rRNA in the ribosomes had informational, structural, and catalytic purposes because it could have coded for tRNAs and proteins needed for ribosomal self-replication  Hypothetical cellular organisms with self-replicating RNA but without DNA are called ribocytes (or ribocells) As amino acids gradually appeared in the RNA world under prebiotic conditions, their interactions with catalytic RNA would increase both the range and efficiency of function of catalytic RNA molecules  Thus, the driving force for the evolution of the ribosome from an ancient self-replicating machine into its current form as a translational machine may have been the selective pressure to incorporate proteins into the ribosome's self-replicating mechanisms, so as to increase its capacity for self-replication 

Heterogeneous ribosomes
Ribosomes are compositionally heterogeneous between species and even within the same cell, as evidenced by the existence of cytoplasmic and mitochondria ribosomes within the same eukaryotic cells  Certain researchers have suggested that heterogeneity in the composition of ribosomal proteins in mammals is important for gene regulation, i e , the specialized ribosome hypothesis  However, this hypothesis is controversial and the topic of ongoing research Heterogeneity in ribosome composition was first proposed to be involved in translational control of protein synthesis by Vince Mauro and Gerald Edelman  They proposed the ribosome filter hypothesis to explain the regulatory functions of ribosomes  Evidence has suggested that specialized ribosomes specific to different cell populations may affect how genes are translated  Some ribosomal proteins exchange from the assembled complex with cytosolic copies suggesting that the structure of the in vivo ribosome can be modified without synthesizing an entire new ribosome 
Certain ribosomal proteins are absolutely critical for cellular life while others are not  In budding yeast, 14/78 ribosomal proteins are non-essential for growth, while in humans this depends on the cell of study  Other forms of heterogeneity include post-translational modifications to ribosomal proteins such as acetylation, methylation, and phosphorylation  Arabidopsis, Viral internal ribosome entry sites (IRESs) may mediate translations by compositionally distinct ribosomes  For example, 40S ribosomal units without eS25 in yeast and mammalian cells are unable to recruit the CrPV IGR IRES Heterogeneity of ribosomal RNA modifications plays a significant role in structural maintenance and/or function and most mRNA modifications are found in highly conserved regions  The most common rRNA modifications are pseudouridylation and 2’-O methylation of ribose 

See also
Context 3: Passage 1:
Whatever Happened, Happened
"Whatever Happened, Happened" is the 11th television episode of the fifth season of ABC's Lost  The 97th episode of the show overall, "Whatever Happened, Happened" aired April 1, 2009, on ABC in the United States  The episode was written by executive producers/show runners Damon Lindelof & Carlton Cuse and directed by "The Man Behind the Curtain" director Bobby Roth In 1977, Kate Austen (Evangeline Lilly) goes to extreme measures to save young Benjamin Linus (Sterling Beaumon) when Jack Shephard (Matthew Fox) refuses to help  In flashbacks, Kate takes care of Sawyer's (Josh Holloway) favor and begins to tell the truth to protect Aaron (William Blanchette) 

Plot
Flashbacks
Shortly after the Oceanic six's rescue, Kate Austen and a baby Aaron visit Cassidy, who is an ex-girlfriend of James "Sawyer" Ford  Kate gives Cassidy and Sawyer's daughter Clementine (Olivia Vickery) money from the severance pay from Oceanic  Cassidy deduces that Aaron is not Kate's baby, prompting her to tell her the truth about everything that happened on the island  Cassidy eventually leads Kate to realize that the reason she took Aaron as her own, is because Kate needed him in order to get over her emotional attachment to Sawyer  After the survivors's confrontation with Ben, Kate momentarily loses Aaron in a supermarket  She recovers it from a woman who bears a strong resemblance of Claire Littleton (Emilie de Ravin), Aaron's mother  After this, she decides to return to the island with the others who left  She leaves Aaron in the care of Carole Littleton (Susan Duerden), Aaron's biological grandmother  Kate also tells Carole that she is returning to the island to find Carole's daughter, Claire, and bring her home 

1977
After Sayid shoots a young Ben, Jin-Soo Kwon (Daniel Dae Kim) wakes up and notices the situation  He brings Ben to the barracks so that his injuries can be treated by Juliet Burke (Elizabeth Mitchell)  Juliet is unable to perform the necessary surgery on Ben and sends James "Sawyer" Ford (Josh Holloway) to retrieve Jack Shephard (Matthew Fox), a spinal surgeon  Meanwhile, Hugo "Hurley" Reyes (Jorge Garcia) and Miles Straume (Ken Leung) debate the nature of time travel  Hurley is convinced that they have changed the past, as in Back to the Future, but Miles tells him that these events always happened in the past  Hurley, however, points that an older Ben didn't recognize Sayid when he was tortured in 2004, as the man who shot him as a kid  Jack, knowing what Ben does in the future, refuses to help, saying that "he already saved him once"  This drives Kate Austen (Evangeline Lilly) to do everything she can to help Ben 
Kate goes to the sick bay where Ben is being treated and donates her blood because she is a universal donor  She strikes up a conversation with Ben's father, Roger (Jon Gries), who is upset that Ben stole his keys and freed Sayid from the jail  Once it becomes clear that Ben will succumb to his injuries without further intervention, Kate decides to take Ben to the Others, the island's native population  Sawyer comes to Kate's aid and they bring Ben to Richard Alpert (Nestor Carbonell), who warns them that if he treats Ben, he will not remember what has happened and will never be the same again  They agree anyway  Another Other urges Richard to inform "Ellie" of his intentions and implies that "Charles" will not be pleased if he finds out, but Richard says he doesn't answer to either of them  Richard then carries Ben into the temple 

2007
After being knocked out unconscious by Sun, Ben (Michael Emerson) wakes up in the infirmary, and is greeted by John Locke (Terry O'Quinn), who welcomes him back to "the land of the living" 

Notes
Passage 2:
SNICK
SNICK (short for Saturday Night Nickelodeon) was a two-hour programming block on the American cable television network Nickelodeon, geared toward older (preteen to teen) audiences, that ran from August 15, 1992 until January 29, 2005  It was aired on Saturdays starting at 8 p m and ending at 10 p m  ET  In 2005, SNICK was revamped as the Saturday night edition of TEENick  Nickelodeon continues to run a Saturday night programming block today, though since the TEENick name was removed from the lineup in February 2009, the block no longer goes by any name 
The block debuted on Saturday, August 15, 1992, with a pair of Sunday favorites (the teen sitcom Clarissa Explains It All and The Ren & Stimpy Show) and the network premieres of Roundhouse (a musical comedy variety series) and Are You Afraid of the Dark? (a horror fantasy-drama anthology series) 

Background
At the time of SNICK's creation, traditional networks such as ABC, NBC and CBS didn't like to program for younger viewers on Saturday nights  The consensus at the time was that viewers who were 50 years of age and older, were the only ones available, since younger viewers traditionally went out on Saturday nights  This would explain why shows such as NBC's The Golden Girls and Empty Nest were the most predominant shows on Saturday nights at the time  Previously on Saturdays, Nickelodeon themselves ceded the 8 p m  timeslot to the vintage sitcoms of the channel's late night programming block, Nick at Nite Then-Nickelodeon president Geraldine Laybourne wanted to expose the myth that there is no audience for kids and teen programming on Saturday nights  Laybourne on that end, was a purveyor of market niche-talk, which was a strategy of programming highly focused programs targeted to specific groups defined by age, gender, race, education, religion or any of a number of other factors  In theory, the audience who would most likely watch SNICK would be too young to be out on the town and subsequently too old to be in bed by eight Laybourne believed that the original shows on the SNICK block would double Nickelodeon's audience on Saturday night by as many as 650,000 to one million viewers  According to Nickelodeon, about one-third of The Ren & Stimpy Show's audience, more than a million viewers, were between the ages of 18 and 35  By early 1993, Nickelodeon according to A C  Nielsen ratings, was the number one network among viewers ages 6–11 on Saturday nights  With a 6 4 age-group rating, Nickelodeon beat Fox's 5 5, NBC's 5 2, CBS' 4 8, and ABC's 3 2

History
1999–2001: SNICK House
On October 16, 1999, SNICK was revamped and was renamed to the SNICK House  With this came a number of changes  The block was now hosted by Nick Cannon, and each week, a celebrity or music group made an appearance  The format was very similar to the former TEENick block, but was more of a party  Each week, kids could go online and vote for their favorite SNICK House Video Picks  The winning music video would then be played during the block  The SNICK House was cancelled on October 1, 2000, making the way for the return of the regular SNICK block 

2001–2002: Elevator Music Era
After SNICK was cancelled on June 30, 2001, with the last program aired being All That, Nick replaced SNICK's normal slot with "Nick Flicks", 90 minute Nicktoon specials followed by The Brothers García  This went on from July 7, 2001 to January 12, 2002 and from June 29, 2002 to September 7, 2002  On January 19, 2002, the brand new SNICK began with a whole new lineup, including a brand new season and subsequently a new cast of All That, which had been on hiatus for a year and a half  Bumpers now featured still pictures of various SNICK stars with a SNICK "talk bubble" above them, with elevator music playing in the background 

2002–2004: SNICK On-Air Dare
Starting in September 2002, SNICK featured a series of On-Air Dare segments featuring members of the All That cast  All but three members of the cast would pull a lever to determine the night's "dare", which one of the three would have to do  The three cast members from All That in each segment would be placed in a glass cylinder and one would be randomly chosen to participate in a dare  If chosen, two security guards enter and grab the cast member (as if he or she was arrested) so they don't escape  This appears to have been based on Fear Factor Some of these dares included singing the National Anthem in a diaper, apple bobbing in a toilet, taking a bath in a tub of raw eggs, eating a couple gallons of blue cheese, being painted with peanut butter and licked by dogs, hanging upside down and being dipped in dog food, having buckets of worms dumped on the cast member's head, drinking a gallon of sweat, sitting in a giant bowl of chili, eating 1,000 toe nails, the cast member putting an entire scorpion in their mouth, the cast member being pecked by hungry chickens, or shaving their school principal's legs 
During this era of SNICK, the SNICK line-ups went through some major transitions that included the phasing out of The Nick Cannon Show and Cousin Skeeter and the addition of a new show, Romeo! 
In 2003, design company Beehive created brand new bumpers for SNICK, featuring an orange splat morphing into a show's character  Instead of saying "SNICK", the announcer said "Saturday Night Nickelodeon" 

2004–2005: Saturday Night on Nickelodeon era and the end of SNICK
On September 4, 2004, SNICK was quietly rebranded as Saturday Night on Nickelodeon  However, the SNICK name was still used during live on-air segments  TEENick Saturday Night replaced SNICK for the 2004–2005 television season and onward  The TEENick block name was dropped in February 2009 in preparation for the launch of a separate channel named after the block, TeenNick  TeenNick launched in September 2009 and much of its programming was sourced from the original TEENick block 

2011: SNICK on The '90s Are All That
In 2011, TeenNick would begin airing 1990s era Nickelodeon shows starting at 12:00 AM Eastern Time under the banner The '90s Are All That  All That and Kenan & Kel are the most prominent and consistent SNICK shows to get reruns  It was announced that the week of December 26, 2011 up until New Year's Eve that TeenNick would air classic SNICK lineups from each year of the 1990s, with a special marathon airing New Year's Eve, all with classic SNICK and Nickelodeon bumpers from the 1990s 
On August 17, 2013, SNICK returned to The '90s Are All That, for its "SNICK-iversary", celebrating its 21st anniversary, reaching drinking age if it were a person  The original lineup was aired (Clarissa Explains It All, The Ren & Stimpy Show and Are You Afraid of the Dark?) with the exception of Roundhouse being replaced by All That 

2017: SNICK on NickSplat
Since the block's 2011 resurgence, SNICK has returned to TeenNick three times  The first two under the block timeslot of The '90s Are All That, which was renamed to The Splat on October 5, 2015, and was renamed once again as NickSplat on May 1, 2017  SNICK's third appearance on TeenNick was to celebrate SNICK's 25th anniversary by airing episodes Saturday nights during the month of August 2017 
August 5, 2017:

12AM The Adventures of Pete and Pete
12:30AM Clarissa Explains It All
1AM Are You Afraid of the Dark?
1:30AM The Ren & Stimpy ShowAugust 12, 2017:

12AM All That
12:30AM All That
1AM Kenan & Kel
1:30AM Kenan & KelAugust 15, 2017:

10PM Clarissa Explains It All
10:30PM Roundhouse
11PM The Ren & Stimpy Show
11:30PM Are You Afraid of the Dark?August 19, 2017:

12AM KaBlam!
12:30AM Rugrats
1AM CatDog
1:30AM The Angry BeaversAugust 26, 2017:

12AM The Amanda Show
12:30AM The Amanda Show
1AM All That
1:30AM All That

Ads and bumpers
Ads and bumpers for SNICK featured the programming block's "mascot," dubbed "The Big Orange Couch," in several locales, including in front of the Midnight Society's campfire, Ren and Stimpy's house, the Roundhouse, as well as various real life and fictional locations  It was retired in June 1999 (however the couch was briefly returned from 2000–2001, in which it was redesigned), when the iconic couch, stuffed with $25,000 and 6,000 cookies, was given away in a contest celebrating Nickelodeon's 20 years on television  In 2006, one of Nickelodeon's celebrities would take over Nickelodeon from Monday to Friday, sitting on the Big Orange Couch 

SNICK line-ups
The following are the shows aired during SNICK for the year listed  Although these are the standard shows aired, some days would see variation in the SNICK line-up 

Home video releases
In August 1993, Nickelodeon released two VHS video tapes meant to recreate the SNICK-watching experience by including episodes from all four of the original SNICK shows: Clarissa Explains It All, Roundhouse, The Ren & Stimpy Show, and Are You Afraid of the Dark?  The tapes also included episodes of the original The Adventures of Pete & Pete shorts in between each SNICK show  as well as SNICK bumpers featuring the Big Orange Couch  The videos were released through Sony Wonder and came in orange-colored cassette tapes 

Volume 1: Nick SNICKS Friendship
Clarissa Explains It All: Season 3 episode "Sam's Swan Song"
The Ren & Stimpy Show: Season 1 episode "The Littlest Giant"
Are You Afraid of the Dark: Season 1 episode "The Tale of the Lonely Ghost"
Roundhouse: Season 1 episode "New Kid In Town"
The Adventures of Pete & Pete shorts "Artie, the Strongest Man in the World," "X-Ray Man," and "Route 34"

Volume 2: Nick SNICKS The Family
Clarissa Explains It All: Season 1 episode "Cool Dad"
The Ren & Stimpy Show: Season 2 episode "Fake Dad"
Are You Afraid of the Dark: Season 1 episode "The Tale of the Hungry Hounds"
Roundhouse: Season 1 episode "You Can't Fire Your Family"
The Adventures of Pete & Pete shorts "The Burping Room," "Mom's Plate," and "The Punishment"

Notes
Passage 3:
CBS Block Party
CBS Block Party (referred to on-air as the CBS Friday Night Block Party) was a programming block that aired on the CBS television network during the 1997–1998 television season  The block was similar to, and was intended as direct competition to, ABC's TGIF lineup and aired on Friday nights from 8:00 p m  to 10:00 p m  ET, and included two former stalwarts of the TGIF lineup  Although the block was canceled after one year, the resulting audience fracture caused what turned out to be irreparable harm to the previously dominant TGIF, eventually clearing the way for CBS to dominate the Friday night lineup beginning in the next decade 

Background
When ABC (a network that was in the midst of an overhaul as The Walt Disney Company took over) canceled the long-running shows Family Matters and Step by Step, CBS picked them up, paying a $40 million sum to earn the rights to the shows, and made them the cornerstones of the new "Block Party " Two new family comedies were added  The first was a new production from Miller-Boyett Productions (the production company behind Family Matters and Step By Step among other TGIF series), Meego  Meego, in addition to being produced by TGIF alumni, also starred a TGIF alumnus: Bronson Pinchot, who previously starred as Balki Bartokomous in Perfect Strangers and as Jean-Luc Rieupeyroux in the sixth season of Step by Step (Pinchot even used a similar accent to the one he used for the Balki character); Meego also featured well-known contemporary child stars Michelle Trachtenberg and Jonathan Lipnicki  The second new series was The Gregory Hines Show, an eponymous sitcom featuring entertainer Gregory Hines; CBS head Leslie Moonves described the Hines show as being more mature and edgy than the other shows in the block, but still family-friendly enough that children could watch comfortably Jaleel White, who played Family Matters star character Steve Urkel, stated that the producers jumped at the opportunity to jump to CBS because ABC was already shifting the TGIF block toward a much more child- and teen-oriented image, moving away from the whole-family approach it had taken at the beginning of its run (White believed being paired with the likes of The Gregory Hines Show was a far better fit than shows such as Muppets Tonight and Aliens in the Family that had been appearing on TGIF at the time), and that they did not believe Disney would give Miller-Boyett as prominent of a role as they had held with ABC before Disney had bought it  CBS, still experiencing aftereffects from the loss of NFL rights and multiple key affiliates to Fox in 1994, saw the purchase as a golden opportunity to draw a younger demographic than it was drawing at the time The CBS Block Party was CBS's second and final attempt to compete with TGIF; in 1992, the network attempted a similar block, albeit targeting an older demographic than either TGIF or the Block Party, that featured The Golden Palace (the continuation of the long-running NBC sitcom The Golden Girls), Designing Women, Major Dad, and Bob  Like the Block Party, this block also failed after one season, and by the end of 1993, all four series had been canceled 

Lineup
8:00pm Family Matters
8:30pm Meego
Replaced midseason with Kids Say the Darndest Things
9:00pm The Gregory Hines Show
9:30pm Step by Step(all times U S  Eastern Time)

Aftermath
None of the shows in the initial lineup lasted beyond that season  Meego lasted a mere six weeks, and The Gregory Hines Show was gone after fourteen  Though the two ABC series were picked up for full seasons, they suffered badly from the network jump, with both series hitting all-time lows in the Nielsen ratings (Family Matters was nevertheless modestly successful enough to beat the show that replaced it in the TGIF lineup, You Wish; the success was short-lived when You Wish was canceled)  Family Matters also suffered due to extensive retooling: Steve Urkel was revamped to tone down his nerdiness, several characters were written out or reduced to guest appearances, and Jo Marie Payton left the show midseason after getting into an altercation with White that nearly turned into fisticuffs (her role as Harriette Winslow was recast with Judyann Elder playing her the rest of the season) 
When the series were canceled, none were afforded a series finale  Kids Say the Darndest Things, however, would continue for two additional seasons, usually paired on Friday nights with a revival of Candid Camera  Kids Say    and Candid Camera would have more sustained success against TGIF, which eventually declined over the next two years until it ended in 2000 CBS has mostly focused on dramatic programming in the time slot since the Block Party was canceled, a programming strategy that has been a relative success in the so-called "Friday night death slot "
Passage 4:
Bob Ferris (Likely Lads)
Robert Andrew Scarborough Ferris is a fictional character in British sitcoms The Likely Lads and Whatever Happened to the Likely Lads? and in The Likely Lads film, played by Bingley-born actor Rodney Bewes  He is single in The Likely Lads, marries Thelma Chambers in Whatever Happened to the Likely Lads? and is still married to her in the film  He works as an electrician and later as a civil engineer  Bob is a long-term friend of Terry Collier 

Early life
Bob was conceived in Scarborough (hence his middle name) by unmarried parents, the day before his father was posted to Catterick by the British Army  Sharing his birthdate with entertainer Bruce Forsyth - as we learn in 'Birthday Boy' - Bob was born on 22 February 1944 into a working-class family  Once he started school, he proved to be an impressionable child, on his own being conscientious, but being easily coaxed into trouble by friends, particularly Terry  At an early age Bob and Terry got into mild trouble, stealing Dinky toys from Woolworths and a hosepipe from a care home  Bob left school to become an electrical apprentice with Ellison's Electrical  While serving his apprenticeship, he also attended night school, eventually becoming a qualified electrician  In the last episode of The Likely Lads, he signs up for the British Army but is discharged for having flat feet  Terry, who only signed up in order to be alongside his friend, is accepted, and goes on to serve in Munchen Gladbach, West Germany 

Personality
Bob is keener than Terry to mask his working-class roots  After marrying Thelma, Bob aspires to be accepted into the middle class, much to Terry's disgust  Bob enjoys playing squash with Thelma, but equally enjoys drinking with Terry  He often blames his drinking, smoking and poor diet on Terry  Although that is in part true, Bob needs little persuasion to stay out drinking with Terry rather than going home to his wife 
Bob is hard-working, conscientious, fairly conventional, and (unlike Terry) ambitious, although he is usually in need of guidance in order to achieve his ambitions  However, he is not particularly assertive, which makes him easily led by his headstrong wife Thelma and equally headstrong friend Terry 
Despite Bob's ambition and enjoyment of his new-found status, he has often defended Terry's "down-to-earth" and "unpretentious" ways, on one occasion even offending one of his own and Thelma's friends in order to defend Terry 
Passage 5:
Whatever Happened to    Robot Jones?
Whatever Happened to    Robot Jones? (simply known as Robot Jones or WHTRJ?) is an American animated television series created by Greg Miller for Cartoon Network  It follows Robot Jones, a teenage robot who attends the fictional suburban Polyneux Middle School in a retrofuturistic 1980s world  Episodes follow Robot Jones researching aspects of human life, including music, facial hair, and gym class  Jones is guided by his three friends, Socks, Mitch, and Cubey  Robot Jones is often smitten with his crush, Shannon Westerburg, a tall, young girl with orthodontic headgear and a prosthesis  In school, Robot Jones interacts with his teachers, Mr  McMcMc, Mr  Workout, and Mrs  Raincoat; the principal, Mr  Madman; and janitor Clancy Q  Sleepyjeans  His arch-rivals, Lenny and Denny Yogman, try to sabotage Jones's research by making school more difficult for him 
Miller's first pilot aired on Cartoon Network on June 16, 2000, as part of "Voice Your Choice Weekend", a contest in which previously unaired pilots were broadcast for viewers to decide which should be given a full series  Even though the Robot Jones pilot ranked second below Grim & Evil in the event, Robot Jones was greenlit for its own series, which premiered on July 19, 2002  The first season voice of Robot Jones was created with a Microsoft Word 97 text-to-speech function  Beginning with the second season, Robot Jones's voice was dubbed over by child actor Bobby Block, and reruns of the first season were re-dubbed with Block's voice overs  The series ended on November 14, 2003, after 13 episodes and a pilot 

Premise
Robot Jones (voiced by a text-to-speech program in the pilot and season 1; Bobby Block in season 2 and season 1 reruns) is a teenage robot living in a fictional early 1980s version of Delaware where robots are commonplace  Robot attempts to learn human nature by attending Polyneux Middle School with his new friends Timothy "Socks" Morton (Kyle Sullivan), a tall boy who loves rock music, Mitch Davis (Gary LeRoi Gray), a headphones-wearing boy whose eyes are hidden by his long hair, and Charles "Cubey" Cubinacle (Myles Jeffrey), a shorter boy who loves video games  He holds a crush on Shannon Westerburg (Grey DeLisle), a girl with a large retainer and prosthetic metal leg 
In each episode, Robot Jones explores a concept faced by average teenagers, such as gym class or competitions  Robot immerses himself in each subject to fully understand it while trying to fit in with his human peers, but this is challenging due to his social ineptitude and others' lack of understanding  As Robot settles in at school, he explores humanoid concepts of his own will  Though the situations he finds himself in are usually at his parents' insistence, others are a result of Robot trying to get closer to Shannon



ground_truth: Ribosomes

prompt_front: Answer the question based on the given passages. Only give me the answer and do not output any other words.

Question: What Friday night programming block included what collective series that includes "Whatever Happened to... Robot Jones?"?

The following are given passages.
Context 3:  An example is in "Summer Camp" when Socks convinces Robot to go camping and Robot discovers the ability to feel jealous  Due to his polite nature and short stature, students at his school tend to ignore Robot or are oblivious to his existence  His good grades, poor social skills, and status as a robot are at odds with Principal Madman (Jeff Bennett), a technophobic principal, Mr  McMcMc (Rip Taylor), a jealous and insecure math teacher, and Lenny (Josh Peck) and Denny (Austin Stout) Yogman, two genius twin brothers  At the end of an episode, Robot reads a "data log entry" about what he learned that day and what conclusions he has arrived at on humanity 
The opening sequence, in which Robot Jones is factory-assembled and inserted into a school bus, is an homage to that of 1980s children's show You Can't Do That on Television  When the title of the show is spoken, a group of young children voice the "Whatever Happened to   " part in unison while the "Robot Jones?" part is done by a Macintosh Macintalk voice known as Trinoids  The first season has children speaking out episode titles while season 2 episode titles are spoken by voices of the characters 

Production
Greg Miller's original series pilot aired on Cartoon Network on June 8, 2000, in a contest featuring 11 animated shorts to be chosen for a spot on the network's 2000 schedule  During the weekend of August 25–27, 2000, all 11 pilots aired as part of a 52-hour marathon called "Voice Your Choice Weekend", in which viewers would vote for their favorite pilots  While Grim & Evil won the contest with 57% of the vote, Robot Jones came in second place with 23% and was given its own series run beginning July 19, 2002 Robot Jones's animation style can be seen as a throwback to 1970s and 1980s cartoons such as Schoolhouse Rock!, with an intentionally messy and rough look; it also strongly resembles classic newspaper comic strips such as Calvin and Hobbes  The series' animation technique is different from most American cartoons from the early 2000s  It was animated with traditional cel animation, at a time when many American cartoons had switched to digital ink and paint  The show was animated at Rough Draft Studios in Seoul, South Korea 
Greg Miller stated in an interview on Facebook that he used a Microsoft Word 97 text-to-speech software on his old Macintosh computer for Robot's voice during production for season one, but after the first season was completed, the executives of Cartoon Network didn't like how it sounded  Bobby Block was chosen to take the role of Robot in season two  Robot Jones's text-to-speech voice was also recorded for production of the second season, but because the voice change happened during the production of those episodes, this voice was never dubbed into the final prints  In that interview, he also said that he would want to do a revival of Robot Jones, but it would be up to Cartoon Network 
The aforementioned interview also revealed that the show was originally planned to be about Robot Jones growing up in the style of The Wonder Years only to take over the world in the style of The Terminator, and when asked how the show would have ended, Greg Miller explained it would end in the show's version of the 1990s with Robot Jones rallying a robot army to attack the human race 

Episodes
Series overview
Pilot (2000)
Season 1 (2002)
Season 2 (2003)
Broadcast
After production ceased on Robot Jones, it aired in syndication before being removed from Cartoon Network's schedule, but episodes were available online on Cartoon Network Video for a short period 
From 2005 to 2006, Robot Jones reran sporadically on The Cartoon Cartoon Show, along with segments of other Cartoon Cartoons from that time period 
Reruns began airing on Cartoon Network's Latin-American sister network Tooncast in 2015  It has also been added to the local version of HBO Max in 2022 
Robot Jones made a cameo appearance on the OK K O ! Let's Be Heroes episode "Crossover Nexus" that aired October 8, 2018, along with other Cartoon Network characters from current and ended shows  This marks the first appearance of Robot Jones's character since the show's cancellation and the first time since the first season where the character's voice was provided by the Microsoft Word 97 text-to-speech programmed voice 

See also
List of fictional robots and androids
List of science fiction universes
Passage 6:
Whatever Happened to    ?
Whatever Happened to   ? is a series of eleven plays broadcast in two series on BBC Radio 4 in 1994 and 1995  They covered the fate of various fictional characters, such as Popeye and Susan Foreman, the granddaughter of the Doctor in Doctor Who  The writer was Adrian Mourby, who in 1997 published a book called Whatever Happened to    ?: The Ultimate Sequels Book, in a similar vein with the further adventures of Frankenstein's Monster, The Artful Dodger, Snow White, Romeo, Big Bad Wolf, Pinocchio, Man Friday, Jane Eyre, Dorothy Gale from The Wonderful Wizard of Oz, and Jim Hawkins 

Episodes
Series one
Series two
Commercial releases
Whatever Happened to Susan Foreman? has been released as an extra on the Doctor Who DVD The Dalek Invasion of Earth 
The Radio 4 series starred Sir Michael Hordern, Jane Asher (as Susan), Lesley Philips (as the Big Bad Wolf), Roshan Seth (as Mowgli) Warren Mitchell and James Grout  The first series won Adrian Mourby the Sony Silver Award for Creative Writing on Radio 

External links
Whatever Happened to    ? at BBC Online
Episode listing on epguides com
Review of the Susan Foreman episode at pagefillers com
Passage 7:
Whatever Happened to Old-Fashioned Love
"Whatever Happened to Old-Fashioned Love" is a song recorded by American country music artist B  J  Thomas  It was released in May 1983 as the first single from the album New Looks  The song was written by Lewis Anderson 
The song was his biggest hit in over five years  "Whatever Happened to Old-Fashioned Love" was the second of three number ones on the country chart  It was his first number one since "(Hey Won't You Play) Another Somebody Done Somebody Wrong Song" eight years before  The single stayed at number one for one week and spent a total of fourteen weeks on the country chart  The song was Thomas' final entry on the pop chart, reaching number 93 
The song was a Top 20 pop hit in the UK for Daniel O'Donnell in the 1990s 

Charts
Weekly charts
Year-end charts
Passage 8:
Richard J  Maybury
Richard J  Maybury (born October 10, 1946) is the publisher of U S  & World Early Warning Report for Investors   He has written several entry level books on United States economics, law, and history from a libertarian perspective  He has written these things in epistolatory form, usually as an uncle writing to his nephew, answering questions  Maybury was a high school economics teacher  After failing to find a book which would give a clear explanation on his view of economics he wrote one himself  Some of his books include Uncle Eric Talks About Personal, Career & Financial Security; a book that is basically the foundation for his other books about the model perspective and Higher Law, Whatever Happened to Penny Candy?; a book that explains the history of the [United States] economic model and how it was based on free-market Austrian economics, Whatever Happened to Justice?; a book about his juris naturalist philosophical viewpoints regarding the foundations of America's legal system, British Common Law, the law of the Franks, and early Christian Ireland 

Early life and family
Richard J  Maybury was born on October 10, 1946 in Hamilton, Ohio, to parents Anthony J , and executive of a West Coast coffee company and co-author of Common Sense Business for Kids, and Ruth M  (née Wellinghoff) Maybury  He married Marilyn N  Williams on August 7, 1967   He has 4 siblings: David, Linda, Jane, and Debra 

Theories
Juris Naturalism
Maybury's viewpoint is "juris naturalism"  Maybury created the term juris naturalism, and called himself a juris naturalist, because he believed no other label was able to fully describe the concept, which he believed is modeled on the viewpoints of many of America's Founders 

Thousand Year War
Maybury has had disagreements with those who say that Muslims are terrorists  In his book, The Thousand Year War, he says that Muslims have been persecuted as much as the Jews by Western civilization through events such as the Crusades, and that they are retaliating after being wronged by the Europeans and the western culture, including the United States government  Maybury states that Muslim nations and people still treat events that occurred centuries ago as modern-day events, and that the recent attacks are retaliatory strikes against what they perceive to be their long-time enemies  He praises the mediaeval Muslim civilizations for their advances in many fields  He also states that Muslims are responsible for preserving the philosophies of ancient people, such as Aristotle 

Maybury's Two Laws
Maybury bases his work on common law, namely

Do all you have agreed to do
Do not encroach on other persons or their property The first law is related to contract law   A contract is an agreement between two or more parties, in which they promise to perform certain actions for and recognize certain rights of the other parties   The second law is related to some criminal law and tort law   Violators of these types of laws have committed acts like theft or violence against other people  (When referencing these two laws, Maybury has at times requested they be known as "Maybury's Laws," and stated exactly as above ) He has also mentioned that there may be another (or others) undiscovered law related to the subjects of law that the two he shows don't cover 

Chaostan
Maybury has declared that nearly a third of the Earth's surface is Chaostan, the land of great chaos  Chaostan is in his view prone to war, financial ill, and tyranny because they never received the Two Laws  The area extends from the Arctic Ocean to the Indian Ocean and Poland to the Pacific, plus North Africa 

The New Axis
Maybury thinks it is possible that some or all of the areas in Chaostan are secretly co-operating, either through political alliance or along ancient ethnic lines  This term was first used in May 1996 

Military Experience
Maybury was a sergeant in the United States Air Force from 1967 to 1971  He served with the 605th Air Commando Squadron in Central America, and with the 75th Military Airlift Squadron in Vietnam  He was also a General Military Training instructor at Travis Air Force Base; and participated in covert operations in South and Central America 

Books
Themes
Maybury's books are marked with emphasis on paradigms, or "models" as he calls them  One of his books, Are You Liberal, Conservative, or Confused is dedicated entirely to this topic  He commonly addresses his letters to an imaginary student named Chris 

Style
All of his books are written in the epistolatory style, as letters from the fictitious Uncle Eric to his nephew  The personal tone of the "letters" convey a certain sense of urgency, yet are remarkably understated compared to other revisionist and contrarian viewpoints 
The books have many illustrations, maps and pull-quotations of historical persons   Though, oddly to some, he rarely if ever quotes recent (20th century) writers   Perhaps it is because he distills the essence of free-market economic thought (what we often call "Libertarian") into fundamental terms that stand on their own   In other words, rather than quoting for instance Henry Hazlitt to support a thesis, he describes an idea in foundational terms that arrive at a conclusion that readers of Hazlitt may find familiar 

Titles
Maybury's in-print books to date are:

Ancient Rome: How It Affects You Today
Are You Liberal? Conservative? or Confused?
Evaluating Books: What Would Thomas Jefferson Think About This?
The Clipper Ship Strategy: For Success in Your Career, Business, and Investments
The Money Mystery: The Hidden Force Affecting Your Career, Business, and Investments
The Thousand Year War in the Mideast
Uncle Eric Talks About Personal, Career, and Financial Security
Whatever Happened to Justice?
Whatever Happened to Penny Candy? 5th Edition
World War I: The Rest of the Story and How It Affects You Today
World War II: The Rest of the Story & How It Affects You TodayAll of his books are published by Bluestocking Press

Journals & Newspapers
EWR
The US & World Investors Early Warning Report (EWR) is Maybury's monthly financial newsletter  EWR seeks to apply the Uncle Eric model to the real world and provide forecasts and warnings of financial changes before they happen  It is very cautious in outlook, and has low toleration of risk 

Freelance Work
Following his military experience, but prior to EWR, Maybury was a freelance writer whose works were featured in the Wall Street Journal, Washington Times, USA Today and other notable publications 

Moneyworld
Maybury was previously global affairs editor for Moneyworld
Passage 9:
The New CBS Tuesday Night Movies
The New CBS Tuesday Night Movies (known as The New CBS Friday Night Movies in its first season) was a weekly 90-minute motion picture made expressly for television   The series aired on CBS from 1971 to 1974  During its first two seasons, the program was similar to ABC's Movie of the Week, which presented a brand-new full-length feature film in a regular weekly time slot with no connecting theme or arc among the films   In the fall of 1972, the series moved from Friday nights to Tuesdays, with its Friday night slot given back to traditional previously released theatrical films under The CBS Friday Night Movies banner (The New CBS Friday Night Movies replaced The CBS Friday Night Movies during its first season) 
During the 1973-1974 television season, CBS revised the series into The New CBS Tuesday Night Movies   In the revision CBS adopted both the ABC and NBC approaches   They developed two rotating series, similar to The NBC Mystery Movie (both produced by MGM Television), and continued to premiere brand new feature-length films as television movies seen on alternating weeks 
The two series-like projects were:

Shaft, a series television version of the 1970s blaxploitation film franchise (itself based on Ernest Tidyman's 1970 novel of the same name), starring Richard Roundtree reprising the role of John Shaft 
Hawkins, starred James Stewart as Billy Jim Hawkins, a rural lawyer who investigated the cases he was involved in, not unlike Stewart's role in the 1959 film Anatomy of a Murder 
Every third week was a television movie The New CBS Tuesday Night Movies was cancelled after the 1973-1974 television season  Television films seen on CBS would be incorporated into its Thursday and Friday night movie programs, beginning with the 1974-1975 season 
Passage 10:
Cartoon Cartoons
Cartoon Cartoons is a collective name used by Cartoon Network for their original animated television series originally aired between 1995 and 2003 and produced in majority by Hanna-Barbera and/or Cartoon Network Studios  Beginning with its inception into cable broadcasting on October 1, 1992, Cartoon Network had focused its programming on reruns of older animated series which it had acquired through its parent company's film library  The Cartoon Cartoons label originated with Fred Seibert's animation anthology series What a Cartoon!, an animation showcase series featuring pilots of original cartoon ideas submitted by independent animators  Dexter's Laboratory was the first such pilot to be greenlit by the network for a full series in 1996  After other pilots were successfully produced into their own series, including Cow and Chicken, Johnny Bravo, and The Powerpuff Girls, the collective Cartoon Cartoons were featured on the network's Friday night programming block, Cartoon Cartoon Fridays from 1999 to 2003  Not all CN original series created around this time were officially recognized as Cartoon Cartoons; Samurai Jack, for example, did not bear the moniker 
The moniker was retired by the network in 2004, and its last surviving series, Ed, Edd n Eddy, ended in 2009 after a ten-year run  Since their heyday, reruns of the Cartoon Cartoons continued to air on The Cartoon Cartoon Show (2005–2008) and Cartoon Planet (2012–2014)  In 2021, the name was resurrected by the network for a new shorts program 

History
Cartoon Cartoons first appeared as shorts on animation showcase series What a Cartoon! in 1995, under the name of World Premiere Toons  The series was produced by Hanna-Barbera and Cartoon Network Studios under the direction of Fred Seibert  Seibert had been a guiding force for Nickelodeon (having overseen the creation of Nicktoons shortly prior to his departure) prior to joining Hanna-Barbera and would establish Frederator Studios years later Through What a Cartoon!, Cartoon Network was able to assess the potential of certain shorts to serve as pilots for spin-off series and signed contracts with their creators to create ongoing series  Dexter's Laboratory was the most popular short series according to a vote held in 1995, and became a full series in 1996  Dexter was retroactively labeled the first Cartoon Cartoon in 1997; however, the network's previous original shows, The Moxy Show and Space Ghost Coast to Coast, were not retroactively given the label 
The Cartoon Cartoon brand was first introduced in July 1997 for the network's Cartoon Cartoon Weekend block  Three more series based on shorts debuted in 1997: Johnny Bravo, Cow and Chicken, and I Am Weasel (the latter two as segments of the same show; I Am Weasel was later spun off into a separate show)  These were followed by The Powerpuff Girls in 1998 and Ed, Edd n Eddy in 1999, and Mike, Lu & Og and Courage the Cowardly Dog in 1999, creating a lineup of critically acclaimed shows  From 1999 to 2003, the Cartoon Cartoon Fridays block was the network's marquee night for premieres of new episodes and series 
In 2001, the network received Time Squad and Grim & Evil  Also in 2001, the show Samurai Jack premiered, but was not officially branded as a Cartoon Cartoon despite airing during the various programming blocks  In 2002, Codename: Kids Next Door became a full series after being chosen in the previous year's Big Pick Weekend  In 2003, Grim & Evil was split into The Grim Adventures of Billy & Mandy and Evil Con Carne; they were the last original series to officially carry the Cartoon Cartoon branding before it was discontinued 
The Cartoon Cartoons were intended to appeal to a wider audience than the average Saturday-morning cartoon  Linda Simensky, vice president of original animation, reminded adults and teenage girls that the cartoons could appeal to them as well  Kevin Sandler's article on them claimed that these cartoons were both less "bawdy" than their counterparts at Comedy Central and less "socially responsible" than their counterparts at Nickelodeon  Sandler pointed to the whimsical rebelliousness, high rate of exaggeration and self-consciousness of the overall output which each individual series managed In October 2003, the live-action Fridays premiered on the network as a replacement for Cartoon Cartoon Fridays  The Cartoon Cartoons bumpers (that appeared before and after episodes of its original series) were dropped after the network's CN City rebrand in June 2004  In August 2004, the block Cartoon Cartoons: The Top 5 was renamed to simply The Top 5  CN still kept the Cartoon Cartoons name around in various forms applying to their older series (such as for The Cartoon Cartoon Show from 2005 to 2008), but since newer shows such as Foster's Home for Imaginary Friends, Camp Lazlo, and Ben 10 were stylistically different from previous shows, the moniker was not applied to them 
However, internationally in places like Asia and Latin America, the moniker continued on until 2007 with shows like Foster's Home, Hi Hi Puffy AmiYumi, Camp Lazlo, and My Gym Partner's a Monkey 

Revival
On April 15, 2021, Cartoon Network announced a new iteration of the Cartoon Cartoons shorts program  On November 24, 2021, the first new Cartoon Cartoons shorts completed production  The first nine shorts include Accordions Geoffery & Mary Melodica by Louie Zong (of The Ghost and Molly McGee and We Bare Bears), Dang! It's Dracula by Levon Jihanian (of Tig n' Seek), Hungy Ghost by Jesse Moynihan (of Adventure Time), Fruit Stand at the End of the World by Rachel Liu, Off the Menu by Shavonne Cherry (of Ren & Stimpy and The Looney Tunes Show), Harmony in Despair by Andrew Dickman (of Looney Tunes Cartoons), Unravel by Alexis Sugden, Mouthwash Madness by Lisa Vandenberg (of Animaniacs), and Scaredy Cat by JJ Villard (of King Star King)  On June 7, 2022, More Cartoon Cartoons completed production  The next seven shorts include The All-Nimal by Nick Edwards (of Apple and Onion and The Fungies), Buttons' Gamezone by Fernando Puig (of The Cuphead Show and Tig n' Seek), Tib Tub, We Need You by Sean Godsey and Mike Rosenthal, I Love You Jocelyn by Tracey Laguerre (Art and Animation Director for brands like Google, Dreamworks, Buzzfeed and more) , Pig in a Wig by Sam Marin (of Regular Show), The Good Boy Report (based on the webcomic of the same name) by Kasey Williams (of Niko and the Sword of Light and Harley Quinn) and Maude Macher and Dom Duck by Kali Fontecchio (of The Looney Tunes Show and Jellystone!) 

Programming blocks
More shows premiered bearing the Cartoon Cartoons brand, airing throughout the network's schedule and prominently on Cartoon Cartoon Fridays, which became the marquee night for premieres of new episodes and shows beginning June 11, 1999  On June 9, 2000, the Cartoon Cartoon Fridays block began to be hosted each week by a different character from a Cartoon Cartoon series, with the first host being Eustace from Courage the Cowardly Dog  The June 9 broadcast also began the first week of The Big Pick, a showcase of cartoon pilots that could become full series based on the results of an online viewer poll  A similar event, The Big Pick II, aired the following year  On October 3, 2003, following a months-long switch to Summer Fridays and Fridays, the block was rebooted under a hybrid live-action format as Fridays, hosted by Tommy Snider and Nzinga Blake, the latter of whom was later replaced by Tara Sands  It aired shows outside the Cartoon Cartoon moniker, such as Foster's Home for Imaginary Friends, Hi Hi Puffy AmiYumi, The Life and Times of Juniper Lee, Camp Lazlo, My Gym Partner's a Monkey, Squirrel Boy, and Class of 3000  The last airing of Fridays was on February 23, 2007 
Cartoon Cartoons: The Top 5 (simply retitled The Top 5 in 2004), an hour-long program featuring a countdown of the week's five "best" Cartoon Cartoon episodes from the network's lineup, ran from 2002 to 2008  From 2005 to 2008, the Cartoon Cartoons label was primarily used for The Cartoon Cartoon Show, a half-hour program featuring episodes of older Cartoon Cartoons that were no longer shown regularly on the network 
The block Cartoon Planet was revived on Cartoon Network from 2012 to 2014, airing in a format similar to The Cartoon Cartoon Show  It featured Cartoon Cartoons such as Dexter's Laboratory, Johnny Bravo, Cow and Chicken, I Am Weasel, The Powerpuff Girls, Courage the Cowardly Dog, Ed, Edd n Eddy, Codename: Kids Next Door, The Grim Adventures of Billy & Mandy, and other original Cartoon Network Studios series such as Foster's Home for Imaginary Friends, Camp Lazlo, and Chowder 

List of series
Precursor
Full series
(+) Indicates that the show originally aired as part of Grim & Evil, and that the 2003-2004 episodes were not produced for the standalone show 

In other media
DC Comics ran an anthology comic based on the Cartoon Cartoons; the series ran from March 2001 to October 2004 for a total of 33 issues 
In the OK K O ! Let's Be Heroes episode "Crossover Nexus", the Cartoon Cartoon logo is shown in the bottom of a wall inside the Cartoon Network headquarters; the Cartoon Cartoon jingle theme song is played when Ben Tennyson (Ben 10) shape-shifts into different Cartoon Network characters 

See also
List of programs broadcast by Cartoon Network
Nicktoons

Notes



ground_truth: Cartoon Cartoon Fridays