Figure 3 Transduction of AAV9- HA - Slc4a11  into the corneal endothelium of  Slc4a11 -/-  (knockout [KO]) animals.  A,  Representative Wes immunoassay of corneal endothelial peelings, from  Slc4a11 -/-  young or older animals, KO(Y) and KO(O), respectively, 4 weeks after AAV9- HA - Slc4a11  injections, using HA antibody. Age-matched noninjected  Slc4a11 +/+  (wild-type [WT]) or  Slc4a11 -/-  (KO) corneal endothelia were used as negative controls, ɑ-tubulin was used as loading control. n = 3, 1 male and 2 female (young animals) and 2 male and 1 female (older animals).  B,  Quantification of data from  A . Mean ± SD. ns = not significant,  P  ≥ 0.1 (1-way ANOVA with Tukey’s multiple comparisons test). AAV9-HA = adeno-associated virus serotype 9 hemagglutinin.