Figure 5. Volcano Plots for ASVs. Sequence variants (each individual point) and their log2foldchange are plotted comparing ( A ) patient eye samples receiving eyedrop treatment compared to controls and ( B ) patient eye samples without eyedrop treatment compared to controls. The log2fold change values are outputs based on DeSEQ2 workflow. Sequence variants found to be relatively abundant with  P  < 0.05 and log2foldchange > 1.0 are denoted in  light blue  (relatively abundant in patients) and  light red  (relatively abundant in controls). Sequence variants found to be relatively abundant with  P  < 0.01 and log2foldchange > 2.0 are denoted in  blue  (relatively abundant in patients) and  red  (relatively abundant in controls). The sequence variants and taxonomic assignments associated with individual data points are noted in  Supplemental Tables S1 ,  S2 .