Fig. 9. AS efficiency of  APXII-Fluc  in the tissues of  APXII-Fluc  plants and  apsi1  mutants. Semiquantitative RT-PCR analysis detected APSI1 mRNA and AS variants (sAPX-II, -III, and tAPX-I types) produced from the  APXII-Fluc  gene in  apsi1  mutants. Semiquantitative RT-PCR analysis (24–28 cycles) was carried out using sets of primers ( Supplementary Table 1 ) in extracts from the rosette leaves, stems, and roots of 4-week-old  APXII - Fluc  plants and  apsi1  mutants (M3 generation). The primer positions are shown in  Fig. 2a . Amplified products were electrophoresed on a 2% (w/v) agarose gel. Equal loading of each amplified cDNA was determined using the control actin2 PCR product. M, molecular marker. Control, the  APXII - Fluc  plants. The experiments were repeated at least 3 times using plants grown independently, and the representative results are shown.