Figure 5 Lactate levels are reduced and lactate transporter expressions are increased with AAV9- HA-Slc4a11  in young animals.  A,  Schematic of the corneal layers and a diagram of lactate movement from the cornea into the anterior chamber with the aid of lactate transporters, monocarboxylate transporter (MCT)1, 2, and 4. 1 , 23 B,  Quantification of lactate levels in  Slc4a11 -/-  animals. n = 3 (young animals, 2 female and 1 male for each group), n = 5 (older animals, 3 male and 2 female for each group). Mean ± SD. ns = not significant,  P  ≥ 0.1 (1-way ANOVA with Tukey’s multiple comparisons test). (C) Representative Wes immunoassay of young and older animals for lactate transporters MCT1, MCT2, MCT4, and loading control ɑ-tubulin.  D,  Quantification of Wes immunoassay results from young animals. n = 3, 1 male and 2 females per group. ns = not significant,  P  ≥ 0.1 (1-way ANOVA with Tukey’s multiple comparisons test).  E,  Quantification of Wes immunoassay results from older animals. n = 3, 2 males and 1 female per group. ns = not significant,  P  = 0.05. For MCT1, the band closest to 66 KDa was used for quantification. Lower molecular weight band was used for MCT4 quantification. AAV9-HA = adeno-associated virus serotype 9 hemagglutinin; MCT = monocarboxylate transporter.