Fig. 4. Sequence specificity of TLN468. ( A ) Schematic representation of the dystrophin gene and its different domains with the position of the selected 40 mutations. ( B ) Quantification of stop codon readthrough efficiency for the 40 most frequent nonsense mutations found in the DMD gene ( n  = 6) in the presence or absence of 80 μM TLN468. A similar quantification was performed with gentamicin (2.5 mM), in the same conditions, to compare the effects of TLN468 with those of gentamicin. ( C ) Box plots of fold change readthrough stimulation for TLN468 (gray) and gentamicin (white) for the 3 stop codons. Center lines show the medians; box limits indicate the 25th and 75th percentiles as determined by R software; whiskers extend 1.5 times the interquartile range from the 25th and 75th percentiles; outliers are represented by dots; data points are plotted as open circles. (UAA  n  = 6; UAG  n  = 7; UGA  n  = 27 sample points). Due to a wider distribution of the points, the Y scale has been modified for the UGA codon.