Fig. 6 Identification and characterization of  SMT  indels in soybean hairy roots from multiple-gRNA construct. (a) Induced mutations of  SMT  gene detecting by heteroduplex analysis. Lines with expected mutations are indicated in red. The red arrowheads indicate DNA band shifts which could be from heteroduplex formation. (b) Indel characterization of representative samples by sequencing. bp, base pair(s). Δ, types of mutation. Different alleles detected in each sample are marked by lower case letters. Red letters are targeted sequences. Blue highlights are PAM sequences. Deletions are presented as red dashes, insertions as green highlights. Mutations at the left target, right target, or large deletions in between are indicated accordingly.