Figure 1 Adeno-associated virus serotype 9 green fluorescent protein (AAV9-GFP) is transduced into the corneal endothelium.  A,  Fluorescence microscopy of mouse eye 2 weeks after AAV9-GFP injection. Note endothelial fluorescence over a uniform background of lens fluorescence. Scale bar –500 μm (left and right images) and 200 μm (middle image). Flat mount of the cornea after GFP antibody staining in green and DAPI in Blue. Scale bar –20 μm.  B,  Cross-section of AAV9-GFP injected and noninjected eyes stained with GFP antibody. No primary antibody sections were only treated with secondary antibody. Scale bar –20 μm.  C,  Corneal thickness measurements of  Slc4a11 +/+  and  Slc4a11 -/-  animals before and 4 weeks after AAV9-GFP injections. Noninjected eyes of the same animals were used as control.  D,  Intraocular pressure measurements of  Slc4a11 +/+  and  Slc4a11 -/-  animals before and 4 weeks after AAV9-GFP injections. Noninjected eyes of the same animals were used as control: n = 3 (2 male and 1 female) per group for a total of 6 animals. ns = not significant,  P  ≥ 0.1 (1-way analysis of variance [ANOVA] with Tukey’s multiple comparisons test).