Figure 2 Vertical fluorescent examination of SI-induced retina damage from Day 1 to Week 3. SI (50 mg/kg, i.p.)-induced retina degeneration in BALB/C mice. (A) Disorganization (asterisks) of retina layers, stained by Hoechst 33342. (B) AF signals in SI-damaged retinas in BALB/C mice. The panel below is overlaid DIC images. Boxed areas are magnified in the inserts. (C) Retina sections stained by RPE65 primary and Alexa Fluor 488 secondary antibodies. (D) Retina sections stained by TUNEL. Asterisks (A,B) indicate ONL disorganization. Scale bars: 50 μm in A; 25 μm in (B-D). RPE, retina pigment epithelium; ONL, outer nuclear layer; INL, inner nuclear layer; GC, ganglion cell layer; AF, autofluorescence; SI, sodium iodate; DIC, differential interference contrast.