
 Fig . 5. ( A ) BPXV infection induces phosphorylation of p38 and eIF4E. Confluent monolayers of Vero cells were mock-infected (uninfected) or infected with BPXV at an MOI of 5. Cells were scraped at indicated time points. The levels of indicated cellular proteins as determined by Western blot analysis ( A1 ) are shown. The blots were quantified by densitometry (ImageJ) and the data are presented as mean with SD. The histograms  A2  and  A3  show band intensity of p-p38α and p-eIF4 E  levels, respectively (normalized with β-actin).  n  = 3 independent experiments. ( B ).  SB239063 blocks p38/eIF4E signaling.  Confluent monolayers of Vero cells were infected with BPXV at an MOI of 5. Inhibitor or DMSO was applied at 3 hpi. Cells were scraped at 24 hpi and levels of the total- and phosphorylated form of p38 and eIF4 E  were determined by Western blot analysis ( B ). The blots were quantified by densitometry (ImageJ) and the data are presented as mean with SD. The histograms  B2  and  B3  show band intensities of p-p38α and p-eIF4 E  in inhibitor-treated and untreated cells respectively.  n  = 3 independent experiments.