Figure 6 Cytoskeletal structure and tight junction architecture are improved with AAV9- HA-Slc4a11 .  A,  Phalloidin staining of corneal endothelia to visualize F-actin cytoskeleton network in  Slc4a11 +/+ , noninjected  Slc4a11 -/-  and AAV9- HA-Slc4a11  injected  Slc4a11 -/- animals.  B,  Quantification of fluorescence intensity of phalloidin staining.  C,  ZO-1 staining of the corneal endothelia to visualize tight junction structure in  Slc4a11 +/+ ,  noninjected  Slc4a11 -/-  and AAV9- HA - Slc4a11  injected  Slc4a11 -/-  animals.  D,  Quantification of cell area.  B  and  D,  Three animals (2 females and 1 male) were used for each group. Seven images were obtained from each group to quantify the average cell density. Mean ± SD. ∗ P <  0.05, ∗∗ P <  0.01, ∗∗∗ P <  0.001, ∗∗∗∗ P <  0.0001. ns = not significant (1-way ANOVA with Tukey’s multiple comparisons test). AAV9-HA = adeno-associated virus serotype 9 hemagglutinin.