Figure 4 Effects of puerarin on ALP activity and mineralized nodule deposition of BMSCs. ( A ) ALP activity quantification of BMSCs stimulated with puerarin for 7 and 14 days (two-way analysis of variance). ( B ) Quantitative analysis of Alizarin red S staining of BMSCs stimulated with puerarin for 4 weeks (one-way analysis of variance). ( C–E ) Alizarin red S staining of control group ( C ), 10 −5  mol/L ( D ) and 10 −6  mol/L ( E ) puerarin group. Scale bar: 200 μm. The columns represent the means. Error bars represent standard deviations. *P < 0.05, **P < 0.01.