Figure 3 RPE degeneration in horizontal view of whole mounts. SI (50 mg/kg, i.p.)-induced RPE degeneration in BALB/C mice. (A) A schematic image of quadrant whole mount with regional indication from center (R1) to peripheral region (R3). R1 is near to optic nerve head and R3 is outside the muscle, and R2 is between R1 and R3. (B) Sclerochoroid/RPE/retina whole mounts were stained with Alexa 488-conjugated phalloidin and Hoechst 33342. Images were taken of RPE layers from center (R1) to peripheral (R3) retinas at Day 1, Week 1 and Week 3. Scale bar: 50 μm. SI, sodium iodate; RPE, retina pigment epithelium.