Figure 3 Analyses of Pi-related traits in the seedlings of T-DNA insertional mutants. Pi concentrations in mutants relative to Col-0 WT in the roots (A) and shoots (B). Twelve-day-old seedlings grown under moderately low Pi conditions (see “Materials and methods”) were sampled. The results are shown in a box and whisker diagram with individual data points superimposed.  n  = 9 biological repeats. The bars within the box plots represent the 25th and 75th percentiles, the center line represents the median, and the whiskers show the minimum and maximum values. Significant differences between mutants and Col-0 were determined by two-sided Student’s  t  test, and  P -values were adjusted for multiple testing using Benjamini–Hochberg correction (*Adjusted  P  <0.05; **Adjusted  P  <0.01). Insertion mutants for the following genes were tested:  GER2  ( AT1G17890 );  EGY3  ( AT1G17870 ); Rhodanese ( AT1G17850 );  C-NAD-MDH2  ( AT5G43330 );  CKL8  ( AT5G43320 );  MYB52  ( AT1G17950 ), and tetratrico peptide repeat (TPR)-like superfamily protein ( AT2G27610 ).