Figure 6 Introduction of {A,C} genome increased  PHT1;1  expression and Pi uptake activity. (A) Relative  PHT1;1  expression and (B) Pi uptake activity in different genetic backgrounds (triangle: maternal line; circle: accessions crossing to Col-0; square: accessions crossing to  pht1;1  mutant). Col-0 maternal line is shown as an opened symbol. Symbols in blue and red indicate the {A,C} and {A,T} accessions, respectively, either the material lines or F1 hybrids. Each symbol represents the average of three to four biological replicates. The mean within the specific group of accessions is indicated as a line. The  P -value was determined by a two-sided Student’s  t  test.