Figure 8 Illustration of our GWAS approach. A, GWAS of two Pi-related traits. Our approach identified the well-known PHT1 Pi transporters and an additional seven genes potentially involved in regulating P acquisition under low Pi. B, Conditional GWAS of PHT1;1/2/3 protein level revealed a biological interaction between SNP locus 1 and locus 2 and an  MYB52-PHT1;1  regulatory module. C, The results of heterozygosity analysis revealed improvement of Pi phenotypes by the introduction of the {A,C} genome into {A,T} lines. D, Different  PHT1;1  haplotypes discriminate soil P contents in the Arabidopsis habitats.