Figure 5 Effect of puerarin on the  ALP  ( A ),  BSP  ( B ),  OCN  ( C ),  SP7  ( D ) expression of BMSCs at 7 and 14 days (two-way analysis of variance). The mRNA expression level of GAPDH was used as internal reference. The columns represent the means. Error bars represent standard deviations. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.