Figure 7 Reduction in paracellular permeability and mitochondrial ROS with AAV9- HA - Slc4a11 .  A,  Quantification of endothelial paracellular permeability with fluorescein dye. n = 3 animals (2 male and 1 female per group), Mean ± SD.  B,  Quantification of mitochondrial ROS in the corneal endothelium using MitoSOX staining. n = 3 animals (1 male and 2 females per group). Mean ± SD.  C,  Wes immunoassay of corneal endothelial peelings using superoxide dismutase 2 antibody.  D,  Quantification of Wes immunoassay results. When multiple bands were present, lower molecular weight band was used for quantification. n = 3 animals (1 male and 2 females per group for young and 2 females and 1 male per group for older animals), Mean ± SD. ns = not significant,  P  ≥ 0.1 (1-way ANOVA with Tukey’s multiple comparisons test). AAV9-HA = adeno-associated virus serotype 9 hemagglutinin.