Fig. 1 Identification of 120 bp of the conserved region of the  Leishmania  minicircle-kDNA in bone marrow aspirates from newborns using polymerase chain reaction (PCR) revealed in 2% agarose gel stained with ethidium bromide. Lane 1: molecular weight ladder (M); lane 2: empty; lanes 3 and 5: bone marrow from newborns 1 and 2, respectively; lane 4: negative control (NC) without DNA; lane 6: positive control (PC) consisting of cultured promastigotes of  Leishmania chagasi  (syn.  Leishmania infantum ).