Fig. 4 Targeted editing of  SACPD-C  gene in soybean transgenic hairy roots. (a) Induced mutations of  SACPD-C  detected by heteroduplex analysis. Lines with expected mutations are indicated in red. The red arrowheads indicate DNA band shifts which could be from heteroduplex formation. (b) Indel characterization of representative samples by sequencing. bp, base pair(s). Δ, types of mutation. Different alleles detected in each sample are marked by lower case letters. Red letters are targeted sequences. Blue highlights are PAM sequences. Deletions are presented as red dashes. Mutations at the left target, right target, or large deletions in between are indicated accordingly.