Fig. 1 gRNA target locations on tested genes and transgenic vector structures. (a) Locations of gRNA target sequences on each tested gene. Target sequences are presented as triangles, gene-specific primers for genotyping as purple ‘L’ shapes. Lengths of PCR products are also indicated in base pairs (bp). (b) T-DNA regions for the targeted editing of  gus  (pCAM1301 _gus- G1-G2_GFP),  SACPD-C  (pFGC5941_ sacpdc -G1-G2_GFP), and both  SACPD-C  and  SMT  genes (pFGC5941_ sacpdc -G3-G4_ smt -G1-G2), respectively. CaMV 35S, Cauliflower mosaic virus 35S promoter. 6xHis, 6xHis affinity tag. NOS-Ter, Nopaline synthase terminator. HygR, Hygromycin resistance gene. AtU6p,  Arabidopsis thaliana  U6 promoter. GFP, green fluorescence protein.  gus , β-glucuronidase gene.  bar , bialaphos resistance gene. LB/RB, T-DNA left and right borders, respectively.