Figure 4 Decreased corneal endothelial cell loss with AAV9-HA- Slc4a11. A,  Corneal endothelial cell density of young and older  Slc4a11 +/+ , noninjected  Slc4a11 -/- , and AAV9- HA - Slc4a11  injected  Slc4a11 -/-  animals visualized with DAPI staining of corneal flat mounts. Scale bar –10 μm.  B,  Quantification of cell density from  A . Three animals (2 female and 1 male) were used for each group. Seven images were obtained from each group to quantify the average cell density. Mean ± SD. ∗ns = not significant,  P  ≥ 0.1 (1-way ANOVA with Tukey’s multiple comparisons test). AAV9-HA = adeno-associated virus serotype 9 hemagglutinin.