Fig. 6. Genome-wide translational consequences of TLN468. ( A ) Ribosome profiling was performed in HeLa cells treated for 24 h with TLN468 (80 μm) (blue), DMSO (black) as a negative control, or G418 (red) (722 μM) as a positive control. Each individual transcript was aligned from the stop codon, and normalized RPFs are indicated by the first nucleotide at the A-site. The vertical red arrow indicates the first position of the stop codon numbered +1, +2, +3. RPFs corresponding to ribosomes reading through TC are also indicated. ( B ) Visualization of programmed TC readthrough on LDHB and MDH1 genes. Average read densities for each condition in RNA-seq and RiboSeq are indicated in gray, exons are represented by coding parts are indicated in large blue boxes, whereas noncoding parts are indicated by a blue line. The 12-nt offset between the read extremities and the position of the ribosomal A-site has been manually corrected to simplify the visualization. The 3 reading frames are shown with stop (red) and AUG codons (green). The reading sense is indicated by white arrowheads. The readthrough region is displayed between the dashed red lines.