Supplementary Fig. 1 Binary vectors for sequential transformation in soybean. (A) Editing exogenous  gus  gene. (B) Editing the endogenous  SACPD-C  gene. (C) Editing both  SACPD-C  and SMT genes with multiple gRNAs. MCSs, expression cassette with multiple cloning sites.  hpt , hygromycin resistant gene.  bar , bialaphos resistant gene. lacZ, Lac operon. GFP, green fluorescence protein. GUS, β-glucuronidase reporter gene. LB/RB, T_DNA left-border/right-border, respectively.