
 Fig . 1. p38 inhibition suppresses BPXV replication. ( A ) Determination of the cytotoxicity (MTT assay). Indicated concentrations of SB239063, SB203580 or equivalent volumes of DMSO, in triplicates, were incubated with cultured Vero cells for 96 h and percentage of the cell viability was measured by MTT assay. CC 50  was determined by Reed-Muench method. ( B ) In vitro antiviral efficacy. Vero cells, in triplicates, were infected with BPXV at MOI of 0.1 in the presence of indicated concentrations of SB239063, SB203580 or equivalent volumes of DMSO. At 48 hpi, infectious virus particles released in the infected cell culture supernatants were quantified by plaque assay. ( C ) Virucidal activity. Indicated concentrations of the SB239063, SB203580 or equivalent volumes of DMSO, in triplicates, were mixed with BPXV (10 6  PFU) and incubated for 90 min at 37 °C after which the virus was diluted (1/1000) and the residual viral infectivity was determined by plaque assay.