Fig. 3 (a)  Leishmania  positive results isolated from freshly ulcers of suspected patients by PCR targeting ITS-rDNA genotype without RFLP. (b) PCR-RFLP observation in Yazd isolates based on ITS-rDNA genotype. Lane 1 (−ve, negative control containing  B suR1 without PCR product), lane 2 (−ve: negative control containing PCR product without  B suR1), lane 3 (+ve, positive control for  L. major ), lanes 4, 5; Yaz32 and Yaz19 ( L. major , common haplotypes): isolated from wet lesions of suspected patients in Esfand-Abad and Chahgir, respectively. Lanes 6–8; MH488992 (Yaz58), MH492002 (Yaz60), and MH488993 (Yaz61) ( L. tropica , three Novel haplotypes): isolated from dry lesions of suspected patients western and southern Yazd, Lane 9; Yaz27;  L. major  isolated from wet lesion of suspected patient, central Yazd. M, 100 bp size marker; M1, 50 bp size marker; –ve, negative; +ve, positive. Fig. 3