Figure 2 Cultivation and characterization of BMSCs. ( A ) Cell morphology of primary BMSCs. Scale bar: 100 μm. ( B ) Cell morphology of passage 3 BMSCs. Scale bar: 100 μm. ( C ) BMSCs were stained with Alizarin red S after osteogenic differentiation induction. Scale bar: 50 μm. ( D ) BMSCs were stained with oil red O after adipogenic induction. Scale bar: 100 μm. ( E – H ) Analysis of BMSCs surface markers expression by flow cytometry. The high expression is on the right side of the central axis. The expression of CD34 and CD45 were negative, while CD90 and CD44 were highly expressed.