Fig. 2. Effects of the 4 selected hits on the endogenous nonsense R213X mutation of  p53 . ( A ) Detection by western blotting of the full-length p53 protein in HDQ-P1 cells carrying the endogenous nonsense mutation R213X. Cells were left untreated (0) or were treated with G418 or the 4 positive hits for 48 h. Membranes were probed with the DO-1 antibody directed against the N terminus of p53, and an anti-actin antibody was used as a loading control. ( B ) Mutant p53 mRNA levels were determined by qPCR ( n  = 3). HDQ-P1 cells were left untreated or were treated with TLN468 (80 μM) or TLN309 (40 μM) for 48 h. The results are expressed relative to the normalized relative amount of mRNA in the absence of a treatment. ( C ) Structures of TLN468 (translectine) and TLN309 (amodiaquine).