Figure 3 Effects of puerarin on the proliferation of BMSCs. ( A ) The growth curves of puerarin-treated groups at different concentration were drawn according to the results of CCK-8 analysis. ( B ) CCK-8 analysis for the proliferation of BMSCs in various concentration of puerarin on day 3 and 5 (two-way analysis of variance). ( C  and  D ) Colony formation assay was performed to test the colony forming capacity of BMSCs. After 10 days, more and larger cell colonies were observed in the experimental group ( D ) than those in the control group ( C ). ( E ) The colony forming efficiency of 10 −6  mol/L puerarin group (one-way analysis of variance). Scale bar: 100 μm. The columns represent the means. Error bars represent standard deviations. *P< 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.