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SUBJECT: Examination of unknown substance'contained in metallic collapsible tube..

Exhibits: Three photographic negatives and four 8" x l0" photographic enlargements of the tube and container as submitted. Two graphs entitled Curve l and Curve 2 representing ultraviolet absorption spectra of the unknown material.

Physical Description of the Unknown: See photos.

Sample as submitted consisted of a collapsible metallic tube contained in clear plastic cylinder.

Tube: The tube was about2" overall and was composed of a body about 1 l2" wide at the base, and a neck and tapering nozzle. The tube body was about an inch in length as received. Unrolled it measured about l". The neck and-nozzle were about 3/L" in length. The appearance of the outer surface of the tube body was dull white and appeared to have been painted. Its surface was soiled. It contained no labelling on its surface. Examination in ultraviolet light revealed no invisihle marks or labelling. Tlie tube as received was uncollapsed. The neck and shoulder contained a dried grey-white exudate. The nozzle opening was corroded and its bore was closed up with decomposed solid material.

The clear plastic cylinder is about 1 5/16" in diameter and about L" in Length. It is divided into two compartments, internally,

C

AM, 3, 5/6

Septembor 15, 1951

TO FROM

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separated by a wall. Each end is capped witha precision ground friction cap. The cap of the smaller compartment ènd contains a minute bore through its center and centered through the wall separating the compartments so that a hypodermic' needle may .be inserted through the small compartment. from the outside into the largecompartment. The rusted frapments of a needle were found in the smali compartment, the sides of which were stained with brownish red smeers: Scrapings of these smeors were taken for possible analysis . They gave strong positive test for iron..

Examination and Analysis: Part l.

September 12-14, 1951

The unknown was opened, physically examined and photographed. The nozzle was penetrated with a hypodermic needle and one droplet of the contents was removed by inversion and gentle pressure on the tubo walls and was placed on a microscopic glass well-slide.

The droplet was a greyish white, somewhat viscous liguid suspension. It was odorless and apparently aqueous. Examination under low 2 power microscope showedit to be heavily laden with particulate matter. A number of clusters of cellular matter with greeriish cell walls were observed at low power. The particulate matter was insoluble in ethanol. Treatment with dilute sulfuric acid (lo) produced large numbers of crystals of calcium sulfate identified positively in polarized light. Slight warning of the slide produced effervescence.

A second droplet was placed in a cuvette containing 2 ml. of
distilled water and an ultraviolet abeorption curve was made in the range
of 220-350 mi. Saples of barbital and scopolamine were run and curves
recorded; they show no absorption characteristics of the unknown. (for curyes,
see graph entitled'curve 1:) The curve of the uhknown is. not characteristic 1

2

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Part 2

Analysis - Attempts to remove more of the sample than the 2 droplets, previously noted, were unsuccessful. After completcly collapsing the tube, the neck and shoulders were carefully scraped to remove solid èxudate. This material was taken up in Jml. of. methanol and labellod Sample l.

The tube itself was cut open with a razor blade revealing a shiry brass colored surface in good state of preservation. Its lower extremity showed a thin coating of a black nubber-like tacky compound. Probably a seal for the folded bottom edge. Therc was no visible evidence of any material on the surface.

The inner surface of the tube was washed with successive portions of absolute methanol totalling several mililiters and the recovered liquid,a milky liquid, was labelled Sample 2.

Samples I and 2 were warmed on a steam bath, cooled to room temperature, centrifuged anialtraviolet spectra were made of each on the Cary Recording Spectrophotometer. Both exhibit absorption in the ultra-violet region of the spectrum but neither curve was characteristic of any chemical or pharmaceutical substance known to the analyst

The solutions were evaporated to dryness and an attempt made to produce a record of their infra-red spectra but the analyst reported to success for lack of sample.

Concluding Remarks.- The analyst has emphasized that although certain data have been presented in this report giving absorption curves, the virtual. lack of sample causes Him to present these data With feservations since it is not

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ccrtain in his view that the scrapings or residual droplets orhwashings represent tho materials desired to be analyzed. The amount of material submitted makes identification,impossible.

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1. The needle is desigred foringection Its gage is not euactly the Sarne as Sto rdard Domsslic nesd

2 The gage of the neebie in deests that it wovld be msed for venepuncturt

3. Subget 'is bung lvomened

4 the hub of the neeble, which is. masie of brass , is a corefully Probuced instruerent The pesee e too bobly do am poscd to hebe a bd- Whose desminer

ful are ugrolly 5.. Yps .042 vutsche deong (on1.0 27" insile diam pinoller Then He neelle is very Slegutly a 19 gogo hippobsrmve neckle. dts outsed deameter is .040" (d1s jrnesent lenyth i 792). Hypo lemic needles generally ane prod in  35",  " 34", 1 and larer Since morivmum length thoboratne coutt a ronsvae

…ー

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10. No in uroopinion, and this iani be proved by Metallagraphict xaminat con it is part os hub of the hypolermic need

Yes. altho no qicher than anrnyectd Probably the design given by syringe. was developed to rever avoid Nransfer or to disguise the anection. Inyection anld are generally put up in glas ampne which are tronsferred to syuinges. It is porsible that wrohout the neeble the inyecti in the tube Gowed faes for some other type also by this means an ingect of medicine coved be given without equpment othir tha a helle. kn ell it is a erude method f pettiny up a potentdruy , if that u what twou intented to contamn

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The Cont ainer

1. no neason apparent to us

3. water proof but cowed not witn hip ya heat wehout fesing. coved n't be Sterlizid bybeat gorcang I donthnow.

6 None

propretanyarno
7 none ppater propre Janyoihe Ghamaoprial duo A
in general use  Physician's arma mentaria mthe

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He Collapsible Tube lluminum (f spectro grum).

ICorld have with Stood neasonoble preosure. (a) yes
(6).no

(e) yes. the tube'is not fragile and woved not. spill its contents unless cut open deffecuet to say. They Coved have been male by hand by a' Carefue technicion

Fire Prof but not heatproof.

of by this is meant: terections, no.

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ht. Micno scopic eamnntion veals hs bains. the neelle  and lenging to it ca not 15no. The manne in which it. was supprnted is. aaoontoas.nol appaent.

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The bos Cloygd w  o mst. thean e Serapings of ust and de cvmposed matter in the arder of 10-25mg. from the outsile surface the nuble gavea positive test for blosd with the Binzidine tot. win I cnnest

Sit.4 This Shoued be investeguted . I suggest that an epert of the be interisewed H-3

Generslly enot thistype. Ainsitogy ue smallpiysewrng tYp typhord notthn kta gaye re Probably potov hoe to Pryoey glyeri ur be onect he whe This depenks on the vescos thicheryels