[PAGE 1]
Two extractions vare porforaod; one usir: absolute otiia and tha sedond uatng 3o" aquecus ethanol.

1. baoluto ethanol: 100 g. of dried sushrsoss fron the 17.7 aaaon colloction wore mscsrated under 300 al. of abs. ethano. in e Taring binnior. The suspanslon was then stirred for 12 .. at poon toapevature and Ciltored. -his extraction was twico .. peatad. The first extract wes yelior, the ohers vory pair. residuo from these extrocts was a wite solsd, with. small o of yollow oll. The oil was removed by saahing with n-poatan: the pentana solution gave 60 ug. o dark brown Poul-smellln; The seni-solid rosiduo, aftor deratting, was vory hysmoscoo, and only partially soluble Is othanol, weight 90 as. Two plss of this gwny solid ware taken orally, first lo5 ag. ar. some hours later, 400 ag. mnly a very questionablo, Fleotin. foot was notiaed. A lho . sample of the pontane solubla wns placod in a gelatin oapauio an: taken, this also gavo css.. \_- ly ho offeat.

It rna thon deaidod to continue the extraction with wa. tho choppod matarial was stirrod with warn alcohol but inodv. .. : ws allowed to go dry, and the temporaturo ross to about So".e 75 matorial was then extreoted onco with 50  alcohol; tnis extr. was inactivo.

It should be noted that during all of tho treatnont vit. - solute athanol, the chopped cusiroom tissus rasinel qulto . ulnr, and driod very rapidly.

B-119

152

xtractton of Hallusiaogonis Pvinoiplo froaailoto gneC

4

[PAGE 2]
2. tractisn with N0, Ethansl:ince it was clear that nìs. otharol was inoffectivo in ponetrating the tissue, 00, othano? vas iried. In thia exparimont, an offort was aade to axliaust mustroon tisaue as quickly as pousible. 50 a. of the dried nus rosas vere maceratad undor 250 ml. of 8os athanol in a Waring Vlendor. The rosulting mixturo vas a pasty mass; no supornatant layer soportod. After ons-halr hour, vith interaittent agitation in .e blendor (toep. 35 - h0°), tho nass was pressod on a suotion Iiiter undor a rubbor das. Ths filtrate was a dark anbor, and slightly fluorescent, This extraction in' the blendor vas repaated three tiges; the fourth extract was very pale yellow. No furthar color was oxtrectod vith vatar. The poolod extracta wore avaporato.! in vucuun bolow rooa tazp. alnost to dryness. A darit ambor syrup undt brovn oil wero presont. Vater and a littlo ethanol were gided und the mixture was defatted with n-pentane. Tho romaining othanol vas than renovod; tho final voluze was Bi al. Thirty al. of this solution (corresponding to 20 ge of dried mushrooas) tas takon orsily. : full-blown effeot, lasting about six hours, was experionced. T visunl pattorns ware perhaps somowhat less vivid; the aubjoctive, enotional roaotion vas perhays intensified.in coaparison to the cxporience with tho driad mushrooas. Algo intansified was the cffret on broathins, which was notod to a loaser extent with the driei us rooas. Proathing was oxceedingly labored, end at one point I wis Casping so hard that I nearly lost conoiouanoss. It should bo noto.l that n full effect vas experionced with l0 g. of dried mushroons; : would judgo that the offoot froa this extract of 20 8. of mushroo-r was dofinitely groator. Thus tho axtraetion appoars to havo boon

quite olfeotive.

B-118

[PAGE 3]
A l0 l. aliqust of the ronaining aqusous solution was 'drio! dosn ia ycug to givo l.05 g. of foan. Ths pentano solution gave l.222 S. of brova cil. It is apparont that a muoh more oxhoustivo extraction was roalissd. The total solids in tho aqusous phaso (total were 8.52 £oi or 17  of tho dry woight.

It may bs acsumad that thez a definitoly positivo roaponce would have boen oxporienced with halr or the 3.2 g. of aolids ingosted. This oan bo translated into'a figure of roughly 30 mg/kg., which might bo takon as a rough bass liza for dospge for an oxtract prepared aa,êsaoribade