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Purooset To determine if three days preireatment with BCL would attenuate the LSD reaction.

Background: Ginzel and Mayer-Gross have reported that two days pretreatment with 3 mg. of BOl,plus an additlonal 1 mg. on the day of the test, attenuated or completety blocked the reaction Induced by smalf doses (40 to 50 mcgm.) of L5D. We attempted to raproduce their experiments, using doses of l-1.5 megm. of LSD, but could show no significant blocklng. Four of the 8 patlents used, however, had very minor reactlons to LSD. The experlment, therefore, was repeeted uslng larger doses of LSD in order to insure a falrly marked degree of reaction.

METHOOS

Sublects: Ten Negro mele postaddlcts, all of whon had been abstinent from opiates for at least six months, voluntecred as subjeets for the experiment, all were in good mentai and physleal conditlon, end' all had experlenced iSD-effects prevlously.

Drugs: Drugs were all glven ln solutlon. One mg. of BOL was administered at 8 a.m. and 6 p.m. for three days prlor to the LSD. On the morning of the test I mg. of BOL was given at

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108

ATTEMPTED BLCCKING OF LSD REACTICN BY PRETREATMENT WITH BOL

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6 otclock, and iSD was glven at 8 o'elock. The dose of LSD was l.5 to 3 megm./kg. LSD doser were choses so as to Tnduce et least a "Grade. 3" reectlon.lhalluclnatlons In all subjectsl.

Designs A randomlzed double-blind deslgn was used. Each patlent recelved the following treatmentst {l} BOL placebo plus LSD, and I2} BOL plus LSD. The comblnatlons of BOL placebo plus LSD placebo, and BOL plus LSD placebo were not studied, parfly because of shortage of materlal and partly because prevlous experlnents have shown that oniy laconsequentlat effacts vouid be expected.

Observatlons: Kneejerks were assessed by determlnlag the angle through which a callbrafed hammer must falt in order to elielt barely perceptible kneejerks. Systollc blood pressure was measured by auseultatory method, Puplllary, diameter was measured In a dart room under constant condltlons of llght and accommodations. These observatlons were obtalned twlce befare and at lntervals of l, 2-1/2,-4, 5-t/2, and 7 hours after LSD.

A nodiflcetion of the Abramson-larvik questlonnatre was admlnlstered by the atde tuice before and at 1/2, 1-l/2, 3, 4-t/2, 6, and 3 hours after LSD. "Citnical-grades" were esstgned on the basts of short mental status exsminatlon performedl at Intervals after LSD.

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Analysls of Data: Areas of the time-actlon curve were calcuiated in the usual way and means and standard errors exfracted from the raw data. Questlons were counted, elimlneting any aaswers that were also reported posltive prior to admlnlstration of iSD.

Resultst Results are shown in the table. There appears to be'a slight trend foward the reductlon of sil aspects of the LSD-reactlon.  The differences, however, ore sltght and not signlflcant, ali being well wtthin the error of measurement.

Conclusloni It was concluded that admtnistratlon of 2 mg. of BOL daily for three days prlor to administratlon of the LSD w1ll not block the response to 1.5 to 3 megm./kg. of LSD.

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Flgures are means on 10 subjects £ standard errors.