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Dr. Eorris Isbell
U.S. Public Hsalth Service Eospital
Lexington, Kentucky

Dear Dr. Isbell:

I have gotten together ali of the information that is presently available on the substance Bufctenine. The source of this is almcst entiraly confined to a talk given by Dr. Ecward Evarts at the Azarican Chenieal Society Modicinal. Chenistry Symposiun at Syracuse which I attenlad last yeok.

Monkeys are regularly given doses of i or 2 ns/ks of tho matericl I.V. At this level the aninaly are auake but totally blind for about an hour. lusole strangth csoss noral dut a profcund ctaxia trevonts valking. The vestibular rochanism:doasn'p seem to te affocted as tho eninal rigits himclf if plocod doan on hés baok. Theso trostsd monkeys are unifornly tractable cnd will not bite even vhon fingers are forced into their nouths. They porsist in this attitudo long after tho blindioss sná atarda vears ofe. lionkeys which were given cbout 6 ns/kg I.V. died in convulsions rosecbling those produced by strychnino aa dià some dogo at about 3 mg/ks I.V. Tee behaviour or dogs at 1 or 2 ms/kg is considerably Cifferent freo that ef the monkeya. Vhereas the monkeys lie quiesly and are very tractable, the dogs howì and indicate an exceelingly disturbed state o2 nind.

Br. Svarts toll of tho rativo use of thia Laterial. Ee said that the Loens wore dried and then pordered and taken as snufe. Prou the concsntraticn or activo principle is tha bann, it was caleulated that if, tho nasel eavity of a person vere cozpletely stusod vith ground bepn, the totol dose per m.s would bo about O.5 =s. Slace tho natives the drug 15 800ive 12 8 C533 02 22r4220 0.1 60 0.5 5 235 2n.

From all that I con gather, the acpivity of this matorial is qualitativoly alaost identical to that of the dzus you have been uoing but Eey be cunsitativaly lers yoesst. I have toos tolt thes 1s is soable and eca be given erally, although in Evarts' expariconts, the I.V. route vas uced.

June 22, 1954

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I hope that I have been able to provide. you vith enough inforzation here to allos sozs prelizinary trials. The problous of supply and stability, etc., voula be vastly sizpliliei if this drug could bo used in lieu of the one you have been investigating.

I'n looking forward to seeing you vhen you are dowm here next zonth.

Pago 2 • Juns 22, 1954

Dr. Isbell

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LSD DOSE-EFFECT DATA

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Notes on LSD Dose-EFFECT Tabie