包埋模具温度对超小活检组织包埋质量的影响 罗添友,唐进,李明峰,张刚 四川大学华西医院病理科(成都 610041) [摘要] 目的 评价包埋模具温度对超小活检组织包埋质量的影响。方法 收集 2019 年 1-3 月在四川大学华西医院内镜下活检及超声科初针穿刺的超小标本 120 例为研究对象,依照随机数字表法分为 H 组和 C 组,每组 60 例。H 组包埋模具温度为 70℃,C 组为常温。比较两组包埋时间、包埋合格率、HE 染色后组织结构完整性、细胞显示效果、网状纤维染色效果及切片等级。结果 与 C组比较,H 组包埋时间延长,包埋合格率增加(P<0.01)。经 HE 染色后, H组组织结构完整性、细胞显示效果、网状纤维染色效果等项目评分高于 C 组(P<0.01)。H 组切片等级高于 C 组(P<0.05)。结论 热包埋模具可提高超小活检组织包埋的合格率及切片质量。 [关键词]包埋模具;温度;超小活检组织;石蜡包埋 Effect of Embedding MouldTemperature on theEmbedding Quality of Ultra-small Biopsy TissuesLuoTianyou, Tang Jin, Li Mingfeng, Zhang Gang. Department of Pathology, West China Hospital of Sichuan University, Chengdu610041, China 【 【 Abstract 】 】 ObjectiveToevaluate the effect of embedding model temperature on theembedding quality of ultra-small biopsy tissues.MethodsAtotal of 120 ultra-small specimens of endoscopic biopsy and initial needle puncture in West China Hospital of Sichuan University from January to March of 2019 were collected as the research objects and divided into Group H and Group C according the random number table methodwith 60 cases in each group.The embedding mouldtemperature of Group H was 70℃,while that of Group C was the normal temperature.Theembedding time and qualificationrateand thehistological integrity, cell display effect, reticular fiber staining effect and slice grade after HE stainingwere compared between the two groups.ResultsCompared with Group C, the embedding time was significantly longerand the embedding qualification rate was significantly higherin Group H (P<0.01). After HE staining, the scores of the histological integrity, cell display effect and reticular fiber staining effect in Group H were significantly higher than those in Group C (P<0.01). The slice grade of Group H was significantly higher than that of Group C (P<0.05).ConclusionThethermal embedding mould can improve the embeddingqualificationrate and slice quality of ultra-small biopsy tissues. 【 【 Key words 】 Embedding mould; Temperature; Ultra-small biopsy tissues;Paraffin embedding 网络出版时间:2019-07-03 09:42:28网络出版地址:http://kns.cnki.net/kcms/detail/51.1705.R.20190703.094