王秀英 (mary at labome dot com)
美国新泽西州普林斯顿合原研究有限责任公司 (Synatom Research)
译者
王秀英 (mary at labome dot com)
美国新泽西州普林斯顿合原研究有限责任公司 (Synatom Research)
DOI
//dx.doi.org/10.13070/mm.cn.2.132
日期
引用
实验材料和方法 2012;2:132
英文摘要
A systematic review of secondary antibodies cited in literature, based on two surveys conducted by Labome.
Goats and donkeys are the predominant host species for secondary antibodies.Rabbit and mouse are the two major target species.Anti-IgG secondary antibodies are commonly cited; a recent study suggests researchers should start to use subsclass-specific anti-mouse secondary antibodies such as anti-IgG1, or anti-IgG2a etc., to increase the detection signals.Western blot, immunocyto- and immunohisto-chemistry are the most common applications for secondary antibodies.Horseradish peroxidase-conjugated secondary antibodies are commonly used in Western blots; while Alexa Fluor 488-conjugated secondary antibodies are in immunochemistry. PE conjugated