Simultaneous Determination of Cryptotanshinone, Tanshinone I andTanshinone IIAin Salviae Miltiorrhizae Radix et Rhizomaby Dual-wavelength HPLCSHI Yan1,2*, XIONG Jing1, HU Xiao-ru1, WEI Feng1, MA Shuang-cheng1, LIN Rui-chao1,2( 1. National Institutes for Food and Drug Control, Beijing 100050, China; 2. School ofTraditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China)[Abstract]Objective: To establish a method for simultaneous determination of cryptotanshinone,tanshinone I tanshinone IIAin Salviae Miltiorrhizae Radix et Rhizoma by dual-wavelength HPLC. Method: TheAgilent ZORBAX SB-C18column ( 4. 6 mm × 250 mm, 5 μm) was used with a mobile phase of methanol-water at1 mL·min- 1, and the detection wave-lengths were set at 245 nm ( tanshinone I) and 269 nm ( cryptotanshinoneand tanshinone IIA) . Result: All calibration curves showed good linear regression in test concentration ranges( r > 0. 999 7) , and the overall average recoveries were in the range o