Effects of Zuo/You-Gui Wan on Mouse Sperm Fertilization AbilityXiao-hui JIANG, Lu ZHANG, Lun ZHANG, Xin LIANG, Hua LU(Reproductive Medicine Laboratory of the Second Clinical College, Chengdu University of traditional Chinese Medicine,Chengdu, 610041)【ABSTRACT】Objective: To explore the effects of Zuo/You-Gui Wan on mouse sperm fertilization ability andstudy on its mechanism. Methods: Normal male mice were randomly divided into the experimental group (n=10)taking Zuo/You-Gui Wan and the control (n=10) taking saline, 1 ml/time, qd×14 d. Both groups were served at theend of 14 d. 1) At 7 d after the last intake time, the male mice mated with the superovulated female mice. After matingsuccessfully, the female mice were executed and obtained 1-cell embryos (fertilized egg) from oviduct. Then theembryo cleavage rate and the percentage rate of blastocysts in vitro were observed. 2) The M II oocytes wereretrieved with the superovulated female mice. The epididymal sperms were obtained at 3 d after the last mating to IVF.Then rate of IVF and blastocysts were primayity observated, the acrosome-intact spermatozoa (AIS) percentage andthe acrosin activity were further observed. Results: Compared with the control, the embryo cleavage rate wassignificantly higher; the IVF rate in the experimental group was significantly higher than that in the control and therewas no significant difference in the rate of blastocysts between the two groups. The acrosin activity was signifi-cantly higher than that in the control, and AIS rate of the two groups showed no significant difference.Conclusion: Zuo/You-Gui Wan can significantly improve the mouse sperm fertilization ability and the mechanismworked potentially by adjusting acrosin activity to influence sperm fertilization ability.Key words: Zuo/You-Gui Wan; sperm fertilization ability; acrosin activity