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出品公司:ATCC
细胞名称:THP-1细胞, ATCC TIB-202细胞, THP1细胞, 人白血病单核细胞
细胞又名:THP1; THP 1; THPI; THP-1(ATCC); Tohoku Hospital Pediatrics-1
存储人:S Tsuchiya
组织来源:单核细胞
疾病特征:急性单核细胞白血病
细胞形态:单核细胞
生长特性:悬浮生长
培养基:RPMI-1640(GIBCO,货号31800022),90%;FBS,10%。
产品目录号:TIB-202
生长条件:气相:空气,95%;二氧化碳,5%; 温度:37 ℃,
传代方法:1:2至1:6,每周2次。
冻存条件:90% 完全培养基+10% DMSO,液氮储存
支原体检测:阴性
安全等级:1
应用:该细胞可以作为转染宿主细胞。
STR:
Amelogenin: X,Y
CSF1PO: 11,13
D13S317: 13
D16S539: 11,12
D5S818: 11,12
D7S820: 10
THO1: 8,9.3
TPOX: 8,11
vWA: 16
备注:
Nucleotide (GenBank) : X70326 H.sapiens MacMarcks mRNA.
参考文献:
Tsuchiya S, et al. Induction of maturation in cultured human monocytic leukemia cells by a phorbol diester. Cancer Res. 42: 1530-1536, 1982. PubMed: 6949641
Skubitz KM, et al. Human granulocyte surface molecules identified by murine monoclonal antibodies. J. Immunol. 131: 1882-1888, 1983. PubMed: 6619543
Cuthbert JA, Lipsky PE. Regulation of proliferation and Ras localization in transformed cells by products of mevalonate metabolism. Cancer Res. 57: 3498-3504, 1997. PubMed: 9270019
Huang S, et al. Adenovirus interaction with distinct integrins mediates separate events in cell entry and gene delivery to hematopoietic cells. J. Virol. 70: 4502-4508, 1996. PubMed: 8676475
Clark RA, et al. Tenascin supports lymphocyte rolling. J. Cell Biol. 137: 755-765, 1997. PubMed: 9151679
细胞图片:

THP-1