北JOUR NAL OF PEKING UNIVERSITY( HEA LTH SCIENCES)京大学学报( 医学版 ) V o1. 46 No. 5 Oct.2014 · 733 · 交联聚乙烯醇栓塞微球的制备与体外性质 杨( 北京大学药学院药剂学系, 北京琴 , 孟文静 , 卢晓静, 吴雅楠 , 仁增顿珠 , 范田园 1 00191) ·论 著· [ 摘方法: 采用乳化化学交联法制备 PVA-Ms, 以红外光谱法考察微球交联产物的结构, 以光学显微镜观察微球的形态、 测定粒径大小, 考察微球的吸水率和溶胀率, 以物性测定仪测量微球的弹性 , 采用创新设计的装置模拟栓塞推注微 球的过程并实时测定推注压力。结果: 红外光谱确证了微球为聚乙烯醇与 甲醛的交联产物。制备的 PVA— Ms 圆 整, 冻干微球的粒径范围为 80 —1 800 Ixm, 平均粒径 574. 2 I xm; 湿微球的粒径范围为 100 ~1 900 p,m, 平均粒径 602. 2 m。冻干微球在生理盐水中2O min 内达到吸水平衡, 平均吸水率为 1 75%, 平均溶胀率为48. 6%。微球具 有良好的弹性, 可顺利经导管推注, 大粒径的微球通过导管的压力较大。结论 : PVA — Ms 的理化性质可以满足栓塞 治疗的要求 , 本研究为系统评价栓塞微球的体外性质提供了方法。 [关键词] 栓塞, 治疗性; 微球体 ; 聚乙烯乙醇 ; 交联试剂 [ 中图分类号] R944. 23 [文献标志码] A [ 文章编号] 1671— 167X (2014)05-073343 6 doi : 10. 3969/ j. issn. 1671— 167X . 2014. 05 . 013 要] 目的: 研究交联聚乙烯醇栓塞微球( polyvinyl alcoho l microspheres, PVA. Ms) 的制备方法及其理化性质。 P rep aration an d i n vitro ev aluati on of crossl ink ed p oly v in y l al coh ol mi c rosp heres for em boli zation Y ANG Shen, ME NG Wen-jing,LU X iao-jing , WU Ya— nan, REN ZENG Dun— zhu, FAN Tian. yuan ( Department of Pharmaceutics,Peking University School of Pharmaceutical Sciences. Beiiing 100191.China) ABSTRACT 0 bjective: To develop and study the properties of crosslinked polyvinyl alcohol microspheres ( PVA — Ms) for embolization.n ethods:The PV A. Ms were produced by emulsion chemical crosslinking method.Fourier ~ ansforIn infrared spectroscopy f FT . IR ) was used to investigate the special functional group s of P V A . M s :the morphology and par t icle size of P V A . M s were determin ed by optical microscope ; the ratio of water absorption and the swelling ratio were also investigated : the c omp ressibility was examin ed by texture an alyzer. A new devic e was designed to measure the pressure of P V A . M s dur i ng their delivery throu gh catheter for embolization .R esu lts :T he c rosslink ing reaction of P V A an d for m ald e— hyde was proved by FT — IR . T he P V A . M s were round with smooth surface. T he average diameter of lyop hilized PV A — M s was 574 . 2 p. m with a range of 80 —1 80 0 with a range of 100 ~1 90 0 LLm.T he average ratio of water ab sorption was 175 % and the swelling ratio was 4 8 . 6 % .T he P V A — Ms were mech anic ally stable with appropr i ate elasticity and delivered th rough the c atheter withou t an y dif f icu lty . and the pressure was h igher f o r larger size of microspheres to be delivered . C on clus ion :PV A — M s prep ared in this study was supposed to b e suitable f o r clinical emboliza— tion acc ording to the physicoc hemical prop erties .T he stu dy p rovides a series of method s to evaluate the properties of microsp heres systemic ally for embolization in vitro. K E Y W OlID S E mbolization ,therapeutic ;M ic rospheres ;Polyvin yl alc ohol ;C ross— link in g reagents m and of wet P V A — M s was 602 . 2 m 血管栓塞术是治疗肿瘤 ( 如子宫肌瘤 、 宫颈癌 、 肝癌等 ) 、 动静脉畸形 、 出血等病症 的有效手段。可 用于栓塞治疗的固体栓塞材料有很 多 , 如聚乙烯 醇 ( polyvinyl Mcohol , PVA) 、 明胶、 海藻酸等 , 由于 PVA 安全 、 生物相容性好 以及具有永久性栓塞等特点 而 成为了最常用的栓塞材料¨J 。 PVA 用于栓 塞治疗 已有 30 多年 的历史 , 国外 已 上 市 的 PVA 栓 塞 剂 有 Ivalon ( Nycomed/ M- PACT ) 、 Truf i ll ( Cordis) 、 PVA ( Cook ) 、 Contour SE ( Boston Scientif i c) , 其 中 Contour SE 为微球型栓塞 剂 , 其他均为颗粒型栓塞剂 。