则原生质体直接加 40ml 5mM MgCl2 4 度下混1h,直接 15000g 离心,沉淀即为内膜。再重复上述步骤一次,洗涤沉淀。
膜蛋白提取:2% SDS 超声下冰浴提取蛋白(体积比 1:10 左右加裂解液,同时加蛋白酶抑制剂),超声 20min (超5s,停10s) 后4度下 15000g 离心 30min,上清即为膜蛋白提取物
参考文献
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