现代泌尿外科杂志 2017年7月第22卷第7期·临床研究·达泊西汀对体外培养精子DNA碎片指数的影响余宏亮,薄立伟(河南省人口和计划生育科学技术研究院男科,河南郑州450002)Effect of dapoxetine on sperm quality and DNAfragmentation index in vitroYU Hong—Liang.BO Li—Wei(Department of Andrology,Henan Population and Family Planning Institute,Zhengzhou 450002,China)ABSTRACT:Objective To explore the effect of different concentrations of dapoxetine intervention on human sperm mo.tility,mortality,and DNA fragmentation index(DFI)in vitro.MethodsA total of 20 sperm samples were divided into 3groups,which were cultured in F-10 culture medium(10×106/mL)by different concentrations of dapoxetine:observationgroup 1(297 ng/mL),observation group 2(498 ng/mL),and negative control group.The sperm motility,mortality,and DFIof all groups were analyzed.Results Three were no differences in the parameters among the 3 groups before and after dapox.etinein treatment(P>0.05).Conclusion Dapoxetinein has no effects on sperm quality and DFl when sperm is treated in vitrofor 24h with dapoxetinein concentration less than 498ng/mL.KEY WORDS:dapoxetine;semen quality;sperm;DNA fragmentation;in vitro