2008年8月广州医学院学报 ACADEMIC JOURNAL OF GUANGZHOU MEDICAL COLLEGE V01.36 No.4 Aug.2008 电针对脑梗死患者血清中谷胱甘肽过氧化物酶活性 及谷胱甘肽含量的影响 (-暨南大学医学院人体解剖学教研室,广东广州510562;2广东佛山南海区中医院针灸科,广东佛山528200) 摘要目的:探讨电针对脑梗死患者血清中谷胱甘肽过氧化物酶(GSH.Px)活性及谷胱甘g/c(GSH)含量的 影响。方法:利用比色法对20例正常人及20例急性脑梗死患者电针治疗前、后血清中GSH含量与GSH.Px活性 分别进行测定,并对比分析。结果:急性脑梗死患者电针治疗后血清中GSH含量(1】.951.67)mg/L、GSH—Px活性 (192.4919.27)IU比治疗前[(2.530.84)mg/L,(122.40_+24.26)IU]和正常组[(4.980.83)mg/L,(144.0635.07)IU] 都显著性增高(P<0.01);电针前与正常组比较,GSH含量、GSH.PX的活性有显著性减低(p<0.01)。结论:电针治 疗能提高人体血清中抗氧化荆的含量,对脑梗死患者因氧化应激生成的自由基有一定的清除效应。 关键词脑梗死;谷胱甘肽;谷胱甘肽过氧化物酶;电针;自由基;脑缺血再灌注;脂质过氧化;氧化应激 中图分类号:R743.33文献标识码:A文章编号:1008一1836(2008)04.0017-03 Effects SerumContents CerebralInfarction ZHANXiang lil,SUBao-guip.PANSan-qian91,SUXuan2LVLai—qin91.HANHuil (1Department ofAnatomyJinan Unwenity Medical College,Guangzhou 510632;2Department ofAcupuncture,Nanhai Hospital ofChinese Traditional Medwine,Foshan 528200,China) Abstract Objective:To investigate electroacupuncture(EA)onactivity Of GSH—Px serumcontents GSHin叫ents cerebralinfarction.Methods:Serum levels 20patients cerebralischemia 20healthy controls were determined comparedbetween twogroups.Results:After treatment 研Ih electroaeupttacture,the serum levels ofGSH(11.951.67)ng/L GSH—Pxactivity(192.49_+19.27)xu isehemicpatients were significandy increased鹊compared baseline[(2.53士0.84)ms/Land(122.d&a:24.26)IU,19<0.01]and controlgroup[(4.980.83)mg/L and(144.0635.07)IU,P<0.01].But GSH—Px activity GSHcontent pre—EApatients were found muchlower than those controlgroup.01).Condusion:EA Was shown human,whichmay scavengefree radicals generated from oxidative stress cerebralinfarction. Key words cerebral infarction;glutathione;glutathione peroxidase;electreacupuncture(EA);free radical;cerebral ischenmia—reperfusion;lipid peroxidation;oxidative stress 在我国,脑血管病、心脏病和恶性肿瘤构成了三 大致死疾病,患病率(719—745.6)/10万人,幸存者中 基金项目:广州市科技计划项目(2007J1.C0051)。 作者简介:湛湘丽(1977.8一),女,主治医师,在读硕士研究生。 研究方向:中枢神经系统的可塑性。 ‘通讯作者:Tel:020-85220243,E—mail:tbgsu@jnu.edu.cn 50%~70%遗留严重残疾[川。因此,康复治疗对脑梗 死患者显得尤为重要。