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罗婧,施海燕,彭文涛,等.恶唑酰草胺及其代谢物的残留分析方法[J].江苏农业学报,2010,26(1):187-191
恶唑酰草胺及其代谢物的残留分析方法
罗
婧,施海燕,彭文涛,王鸣华
(南京农业大学植物保护学院农药科学系,农业部作物病虫害监测与防控重点开放实验室,江苏南京210095)
摘要:建立了能同时测定水稻及环境中恶唑酰革胺及其3种代谢物残留量的高效液相色谱(HPLC)法。以
乙腈和水的混合液作为提取溶剂,硅胶层析柱净化,采用高效液相色谱法以乙腈一水作为流动相,梯度洗脱,紫外检测器224nm检测。结果表明:恶唑酰草胺及其3种代谢物的线性范围为0.5~20.0m#L,相关系数达0.999
0.9990.02
3~
9;4种化合物在土壤、稻秆、糙米和稻壳中的最低检测浓度均为0.10mg/kg,在稻田水中的最低检测浓度为mg/kg,平均回收率均为78.2%~106.9%,变异系数为2.0%一11.7%,符合农药残留测定要求。
关键词:恶唑酰草胺;水稻;高效液相色谱;残留分析中图分类号:X592
文献标识码:
A
文章编号:1000-4440(2010)01-0187-05
ResiduesAnalytical
LUOJing,
t
Department
MethodsforMetamifopand
WANGMing—hua
University.Key
Its
Metabolites
SHIHai—yan,PENGWen—tao,
Pesticide
fdScience,Collegeof
Plant
Protection,Na畸tngAgriculturalLaboratory(JJMonitoringandManagementofCropDis-
eases
andPest
Insects,MinistryofAgriculture,Nanjing210095,China)
Abstract:Thehighperformanceliquidchromatographicmethod
metamifopandthree
WaS
developedforthesimultaneousdeterminationof
extractedwith
a
a
major
metabolitesinriceandenvironment.Residues
a
weremixtureofaeetonitrileand
ace—
water,followedbycleanupwithtonitrileand
water
as
silicagelcolumllanddeterminedbyHPLCwith
at
lineargradientelutionprogramof
themobilephaseandultravioletdetection
were
224nm.Theresultsshowedthatthelinearrangeofmet
to
amifopanditsmetabolites
from0.5mg/t。to20mg/L,withthecorrelationcoefficients
being0.9993
0.9999.The
were
minimumdetectableconcentrationofthe4analytesinsoil,ricestraw。ricehulls
and
unpolishedgrainsamples
78.2%to
all0.10
mg/kgand0.02mg/kginpaddy
water
samples,respectively.Averagerecoveries
were
106.9%,withthecoeffi—
cientsofvariationrangingfrom2.0%to11.7%.Theresultsmeettherequirementofpesticideresidueanalysis.
Keywords:
metamifop;rice;highperformanceliquid
chromatography(HPLC);residue
analysis