-0.8 -0.6 -0.4 -0.2 0.0 0.2 -40 -20 -0.6-0.4 -0.2 0.0 -40 -20 -0.6-0.4 -0.2 0.0 -10 -5 vs.SCE) vs.SCE) vs.SCE) 氮掺杂石墨烯修饰玻碳电极(N-graphene/GC)(图A)、石墨烯修饰玻碳电极(graphene/GC)(图(GC)(图C)在含有0.2mmol/L 溶液(曲线b)和不含有0.2mmol/L 溶液(曲线a)的循环伏安图。Figure Voltammetricresponse electrocatalyticreduction 0.2mM N-freegraphene bareI17,第十三届全国有机分子电化学及工业学术会议论文摘要 Corresponding author: cxcai @njnu.edu.cn GC responseswere recorded -saturatedPBS (0.1 pH7.4) without(curves 0.2mM Scanrate 100mV/s. 在外界刺激作用下,嗜中性粒细胞内的蛋白质激酶C(pKC)会发生活化,导致呼吸爆发,表现为释放出大量H N-graphene/GC电极能用于监测这一释放过程。加入PMA(最终浓度为100 ng/mL)后,N-graphene/GC 电极的响应电流 迅速增加(图2),表明有H 从细胞中释放出来;最大电流约为0.56μA,相当于每个细胞释放出(2.1 10–15 mol 10–15 mol),说明该电极可以准确地监测细胞释放H 机理方面的信息。细胞受PMA 刺激后, N-graphene/GC 电极的响应电流先略有增加(图 ,并发生胞吐作用,进而在膜表面形成了融合孔,导致少量 s;尔后响应电流迅速增大,并在约13 后达到最大值(图2,第II阶段),说明融合孔迅速扩张并造成大量H 从细胞中快速释放出来。II 50100 150 200 50100 150 200 250 PMA(ng/mL) 50100 150 200 250 PMAfMLP AA ADP 200400 600 MCF-7RAW264.7 Neutrophil 50100 150 MCF-7RAW 264.7 Neutrophil culturedwithout N-graphene cultured N-graphene/GC电极响应细胞中H 曲线。曲线(a):溶液中不含过氧化氢酶(300U/mL);曲线(b): 溶液 中含过氧化氢酶。PMA 浓度100 ng/mL。 Figure Typicalamperometric responses N-graphene/GCelectrode releasingfrom neutrophils induced PMA(100 ng/mL) catalase(300 U/mL). References commonbiology ageing.Nature, 2007, 448, 767-774. lipidperoxidation biologicalsystems. Ann Rev Nutr, 1985, Redox-dependentsignal transduction. FEBS Lett, 2000, 476, 52-54. Electrochemical measuring releasingprocess hydrogenperoxide from living cells based nitrogen-dopedgraphene Ping Wu, Chenxin Cai* JiangsuKey Laboratory NewPower Batteries,, College MaterialsScience, Nanjing Normal University, Nanjing 210046)