四级
UID 77185
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积分 561
帖子 781
信誉分 100
可用分 4746
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阅读权限 255
不管做WST-1和已过时的MTT,还是做Elisa、测RNA纯度等都会用到酶标仪,而且从前都认为OD值在2以内才满足线性关系,但是我看了一下Thermo Scientific Varioskan Flash 酶标仪的说明书,里面说96孔板只要0-4的吸光度值都满足线性关系
四级
UID 74485
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积分 521
帖子 701
信誉分 100
可用分 4361
专家分 0
阅读权限 255
不管做WST-1和已过时的MTT,还是做Elisa、测RNA纯度等都会用到酶标仪,而且从前都认为OD值在2以内才满足线性关系,但是我看了一下Thermo Scientific Varioskan Flash 酶标仪的说明书,里面说96孔板只要0-4的吸光度值都满足线性关系
=================================================================
Linearity (Photometry)
0 to 4 Abs, (96-well plate) at 450nm, ±2%
0 to 3 Abs (384-well plate) at 450nm, ±2%
Accuracy (Photometry)
±2% or 0.003 Abs, whichever is greater, at 200 to 399nm (0 to 2 Abs)
±1% or 0.003 Abs, whichever is greater, at 400 to 1000nm (0 to 3 Abs)
Precision (Photometry)
SD