本论文发表于基因组学与应用生物学杂志,属于农业相关论文范文材料。仅供大家论文写作参考。
AbstractNucleosomeisthefundamentalrepeatingunitofeukaryotchromatin.Bychangingtheaccessoftrans-actingfactorstotheirtemplateandcontrollingthedegreeofchromatinopeningorfolding,nucleosomeassemblyregulatesmanybiologyprocessesoccurredinchromatinlevel,suchasDNAreplication,RNAtranscription,DNArepairandDNArecombination.Histonesarethebasiccomponentsinnucleosome,andinvestigatingtheeffectofhistoneconcertrationonnucleosomeassemblyisthebasisforunderstandingthedymanicofnucleosomeassembly.FourhistoneswereexpressedandpurifiedfromEscherichiacoliBL21cellscontainingpET-histoneexpressionplasmids.Thenucleosomeswereassembledwithdifferenthistoneconcentrationusingthesalt-dialysismethodinvitro.Basedonthequantificationalresultsofefficiencyofthenucleosomeassemblyandhisoneconcentration,adynamicalmodelofhistone-dependentnucleosomeassemblywasproposed.Theexperimentalresultsfoundthattheralationbetweenefficiencyofthenucleosomeassemblyandhistoneconcentrationshowedtypicallinearity,andtheregressioncoefficientwas0.963intheequationoflinearregression.Thereactionrateconstantkofnucleosomeassemblyon601DNAtemplatewas1.49×10-5mL·h·μg-1.VerificationtestonCS1DNAsequenceshowedthattheregressioncoefficientofequationoflinearregressionwas0.989andthereactionrateconstantkonCS1DNAtemplatewas1.52×10-5mL·h·μg-1.ThereactionrateconstantkindynamicalequationcanbeemployedtoevaluatetheabilityofDNAsequencestoassemblenucleosome,efficencyofnucleosomeformationonbetweenhistoneand基金项目:本研究由国家自然科学基金项目(31760247;61671256;61662055)和内蒙古自然科学基金项目(2017MS0303)共同资助引用格式:ZhangF.H.,GuoM.X.,ZhaoH.Y.,andCaiL.,2019,Dynamicmodelofhistone-dependentnucleosomeassemblyinvitro,JiyinzuxueYuYingyongShengwuxue(GenomicsandAppliedBiology),38(7):2996-3001(张凤慧,郭明欣,赵宏宇,蔡禄,2019,体外装配核小体过程组蛋白浓度依赖的动力学模型,基因组学与应用生物学,38(7):2996-3001)体外装配核小体过程组蛋白浓度依赖的动力学模型2997histonemutantorhistonevariants.Thedynamicalmodelmaybehelpfulforunderstandingtheregulationmechanismofnucleosomeassembly,nucleosomepositioningandchromatinstructure.KeywordsHistone,Nucleosomeassembly,Chromatinstructure