CSF第一管:生化(蛋白、糖)
CSF第二管:微生物(革兰染色,培养,PCR,快速诊断检测如乳胶凝集试验)
CSF第三管:血液学(白细胞计数)
若无充足样本,则优先保证细菌培养,若样本量充足应按以下顺序送检:
Remove CSF (1 ml minimum, 3-4 ml if possible) and collect into sterile screw-cap tubes. If 3-4 ml CSF is available, use 3 separate tubes and place approximately 1ml into each tube.
Transport the CSF to a microbiology laboratory within 1 hour for culture and analysis.
If possible, three tubes (1 ml each) of CSF should be collected for microbiology, chemistry, and cytology.If only one tube of CSF is available, it should be given to the microbiology laboratory.Because the presence of blood can affect cultures of CSF,if more than one tube of CSF is collected from a patient, the first tube collected (which could contain contaminating blood from the lumbar puncture) should not be the tube sent to the microbiology laboratory.
只强调了第一管不能送病原学检测(除仅有一管标本外)
胶乳凝集试验、微生物检测
MedscapeWhy are four tubes of cerebrospinal fluid (CSF) sample drawn in suspected meningitis and whe