中华泌尿外科杂志CHINESE JOURNAL OF UROLOGY1999年　第20卷　第6期 Vol中华泌尿外科杂志 CHINESE JOURNAL OF UROLOGY 1999年　第20卷　第6期 Vol.20 No.6 1999人正常尿路移行上皮细胞无血清培养汤洋　畅继武　隋志芳　马腾骧　　【摘要】 　目的　建立一种比较简便的人正常尿路上皮细胞无血清培养方法。 　方法　标本组织块用冷消化法分离出尿路上皮细胞, 培养于无血清生长培养基。 观察传代细胞形态及生长情况, 并用TGF-β1抑制细胞增殖试验检验细胞在有血清或无血清培养基中对TGF-β1的反应。 　结果　培养的总成功率为73%, 细胞具有典型的移行上皮细胞形态学特征, 细胞能在24小时后增殖进入对数生长期, 传代9~11次后开始衰退。 另外, 无血清培养体系比含血清培养体系更能反映出TGF-β1对细胞抑制作用。 　结论　此培养体系具有简单、 短期内可扩增出大量纯净上皮细胞、 应用范围广和培养成功率高的特点。 　　【关键词】 　细胞学技术　　细胞, 培养的Culture of human normal urothelial cells with serum-free mediumTANG Yang,CHANG Jiwu,SUI Zhifanget al.Department of Urology,The Second Hospital,Tianjin Urological Institute,Tianjin 300211　　【Abstract】 　Objective　To establish a relative simple and convenient serum-free culture method of human unthelial cells.　Methods　Epithelial cells were isolated from sample tissue by cold trypsinization and cultured in serum-free growth medium.Growth and morphology of passaged cells were observed.Moreover,cells reacting to TGF-β1 was cheked with TGF-β1 inhibiting cell growth test in serum-free or containing serum medium.　Results　Total successful rate of culture was 73%.Cultured cells had typical morphological features of transitional epithelium and rapidly proliferated into logarithm growth period.After 9~11 passages,cells began senescence.The changes of cell proliferation in serum-free medium rather than serum containing medium reflected the TGF-β1 effect.　Conclusions　This culture system was simple and convenient,amplifying a lot of pure epithelial cells in short term with more applicability and high successful culture rate. 　　【Key words】 　Cytological technics　　Cells,cultured　　培养人正常尿路移行上皮细胞对认识移行细胞分化和增殖机制, 深入了解膀胱肿file:///E|/qk/zhmnwk/zhmn99/zhmn9906/990615.htm(第 1/5 页) 2010-3-23 19:55:20万方数据 中华泌尿外科杂志CHINESE JOURNAL OF UROLOGY1999年　第20卷　第6期 Vol瘤成因和用组织工程技术构建人工膀胱是不可缺少的研究手段[1, 2]。 人正常尿路移行上皮细胞被公认为是较难培养的上皮细胞之一[3], 对培养的微环境要求高[4]。