Abstract　Specific　peptide　molecules　can　be　obtained　by　phage　display　technology,　and　they　are　widely　used　in　food　and　environmental　monitoring,　purification　and　separation　of　biological　macromolecules,　biosensor,　imaging,　drug　delivery　and　targeted　therapy　et　al.　However,　the　traditional　phage　display　technique　for　the　screening　of　specific　peptides　has　the　disadvantages　of　biased　amplification,　tedious　operation,　low　throughput,　time-consuming　and　high　cost.　Therefore,　there　is　urgent　need　to　establish　and　develop　a　simple,　rapid,　efficient,　high-throughput　and　low-cost　method　for　screening　peptide　based　on　phage　display　technology.　Combination　of　the　droplet　microfluidic　chip,　with　the　advantages　of　small　volume,　no　sample　diffusion,　sample　and　reagent　consumption,　no　cross　contamination,　stable　reaction　condition,　short　reaction　time　et　al,　and　introducing　the　fluorescence　analysis　into　the　selection,　a　rapid　and　efficient　droplet　phage　display　has　been　developed　for　screening　speci