关键词: 人参皂甙;创伤 /失血性休克;免疫调节;大鼠中图法分类号: R605. 97l文献标识码: AEffects of ginsenoside on immunomodulation after traumatohemorrhagicshock in ratsLIN Jing-hua, CHEN Hui-sun, HU De-yao, LIU Liang-ming (Research Institute of Fieid surgery, Daping Hospitai, Third MiiitaryMedicai University, Chongging 400042, China)Abstract: Objective To study the moduiation effects of ginsenoside (GS) on the immune func-tion in traumatohemorrhagic shock in rats. Methods The study was divided into in UiUo and in Uitropart. In the in UiUo sutdy, the rats were pretreated with gensinoside 2. 5 mg /l00 g for 3 days and infiict-ed with traumatohemorrhagic shock. The rats were kiiied onthe next day and the functions oftheiriympho-cytesandmacrophageswereobserved. Inthe inUitrostudy, theratswereinfiictedwithtraumatohemorrhag-ic shock and kiiied on the next day. The iymphocytes in the spieen and the macrophages in the peri-toneai cavity were isoiated and purified. Their immune functions after the treatment of l to l00 #g /miofGS were studied. Results In the in UiUo study, the pretreatment ofGS resuited in enhanced proiifera-tive capacity, eievated IL-2 reieasing and increased IL-2R expression in spienocytes and enhanced anti-gen presentation, increased TNF reieasing and phagocytosis in peritoneai macrophages. In the in Uitrostudy, administration of GS aiso improved the functions of spienocytes and peritoneai macrophages.Conclusion Our findings suggest that GS administration in UiUo and in Uitro is abie to improve the im-mune functions of iymphocytes and macrophages in traumatohemorrhagic shock.Key words: ginsenosideg traumatohemorrhagic shockg immunomoduiationg rat324第 22 卷第 4 期第 三 军 医 大 学 学 报Voi. 22, No. 42000 年 4 月ACTA ACADEMIAE MEDICINAE MILITARIS TERTIAEApr. 2000 表 1GS 对创伤失血性休克 1 d 大鼠脾淋巴细胞功能的影响 ( ! = 10)Tab 1Effect of GS on splenocyte function 24 hours aftertrauma-hemorrhagic shock ( ! = 10)GroupProIiferation (min- 1)IL-2 (U/mI)IL-2R!( !495)ControI5612.2 1 151 .446.83 1 0.560.350 1 0.01T-HS3002.2 1 164.21! !2.32 1 0.33! !0.169 1 0.02! !T-HS + GS4124.3 1 186.00##5.58 1 0.26#0.282 1 0.02#T-HS: Trauma-hemorrhagic Shock;!!: " < 0.01#$ controI; # : " < 0.05, ## : " < 0.01 #$ T-HS血性休克免疫功能的调节作用。!材料与方法l. l动物Wistar 雄性大鼠, 体重 200 ~ 280 g, 由第三军医大学附属大坪医院野战外科研究所实验动物中心提供。