现代食品科技 Modern Food S cience and Technology 2013, Vol.29, No.1 复合乳酸菌粉冲剂增强免疫力作用的研究 朱韶娟 ,张立彦 (1.华南理工大学轻工与食品学院,广东广州 510640) (2.广州雅芳保健品制造有限公司,广东广州 510900) 摘要:研究了复合乳酸菌粉((嗜酸乳杆菌+乳双歧杆菌+低聚木糖)的益生菌冲剂在增强儿童免疫力方面的作用,研究方法依 照《保健食品检验与评价技术规范》(2003版)之“增强免疫力功能检验方法”。结果表明:对SPF级昆明种小鼠经口每日分别给予该 复合益生菌产品0.25、0.50、1.50 g/kg· BW,相当于人推荐日服量的5、10、30倍共4周,实验显示:绵羊红细胞诱导的小鼠迟发型变 态反应试验和ConA诱导的小鼠脾淋巴细胞转化增殖试验结果呈阳性, 说明该样品具有增强细胞免疫功能作用 (P<0.05); 同时受试动物 NK 细胞活性试验结果呈阳性,说明该样品具有显著增强NK细胞活性作用(P<0.01)。实验证明摄入该复合益生菌产品具有增强免疫力 的功能作用。 关键词:复合乳酸菌;低聚木糖;增强免疫力 文章篇号:1673-9078(2013)1-77-80 1,2 1 Study on Enhancement of Complex Probiotics Granules on Children Immunity ZHU Shao-juan1,2 , ZHANG Li-yan1 (1.College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China) (2.Avon Healthcare Products Manufacturing (Guangzhou) Ltd., 510900, China) Abstract: The efficacy of probiotics (Lactobacillus acidophilus + Bifidobacterium lactis ) and xylooligosaccharide complex granules on enhancing junior immunity was studied. According to “Technical standards for testing & assessment of health food (2003) - Method for the assessment of enhancing immunity function”, the test results indicates that, after 4 weeks mice( SPF grade from Kunming) test with daily dosage of 0.25, 0.50, 1.50 g/kg· BW (comparatively as 5, 10, 30 times of recommended human daily dosage) by oral. Both the results were positive for the test of mice. Delayed hypersensitivity induced by SRBC and conversion proliferation reaction of mice splenic lymphocyte. It can claim that the product could enhance the cell immunity (P<0.05). It is positive for NK cell activity test. The sample could enhance NK cell activity significantly (P<0.01)). It showed that the product had the function of enhancing immunity. Key words: probiotics complex; xylooligosaccharide; enhancing immunity 益生菌的增强免疫力作用已被广泛认同。