个地理种群红条毛肤石鳖COI基因的遗传多样性分 Abstract[Objective] geneticdiversity COIgene Acanthochitonrubrolineatus from littoralgeographical populations China.[Method]COI gene 80samples A.rubrolineatusfrom geographicalpopulations were amplified polymerasechain reaction PCRtechnology).And we used DNASP5.1001,MEGA7.0 othersoftwares fragmentbase content,distance othergenetic diversity parameters constructingphylogenetic trees geneticdiversity data.[Result]The length COIgene fragment 659bp,COI gene encodingregion averagecontent 20.4%,41.7%,14.9%,23.0%respectively ,showing phylogenetictrees Acanthochitonacrinite,we found allhaplotypes A.rubrolineatuspopulations Chinacoasts were clustered twoapparent geographic lineages phylogenetictrees,named Northerngroup Southerngroup,the genetic distance ranged from 0.000 66 0.08785.The results haplotypegenetic diversity reflected nucleotidediversity low.[Conclusion]Therewere obvious differences between northerngroup southerngroup southerngroup should protectedseparately t