Fig. 1　Observation of cell shape under inverted phase constrast m icroscope　○ a　The primary culture of canine bladder transitional epithelial cells,the clones were formed after 48 hours (× 100) 　○ b 　The primary culture of canine bladder transitional epithelial cells w ere about 80% 290%confluence after 7 days (× 40) 　○ c　The 4th passage of cultured canine bladder transitional epithelial cells arranged like slabstone (× 200) 　○ d　The7th passage of cultured canine bladder transitional epithelial cells had cell aging appearance (× 200) 　Fig. 2　The growth curve of the 3rd passage ofthe can ine bladder transitional epithelial cells　Fig. 3　I mmunohistochem ical sta in ing of the cultured can ine bladder transitional epithelial cells withbroadly reacting anti-cytokeratin antibodies(SABC × 200) 　○ a　The 3rd passage of cultured canine bladder transitional epithelial cells were stainedpositively　○ b　The normal canine bladder urothelium in positive control was stained positively　○ c　I mmunohistochem ical staining of blank controlwas negative　○ d　The normal canine bladder smooth muscle cell in negative control was stained negatively　Fig.can ine bladder transitional epithelial cells　○ a　The cellswere characterized by well2developedm icro2filament and desmosome junction (TEM × 12k)4　Ultrastructure of cultured　○ b　Small and immature transitional epithelial cells which differentiated highly(TEM × 4k)・1421・中国修复重建外科杂志 2007 年11 月第 21 卷第 11 期 4　参考文献1A tala A. Regenerative medicine and urology. BJUInt, 2003, 92(S1): 58267.2A tala A , Bauer SB, Soker S, et al. T issue2engineered autologousbladders for patients needing cystoplasty. L ancet, 2006, 367(9518): 124121263.3韩平, 魏强, 杨宇如. 组织工程尿道重建的研究进展. 中国修复重建外科杂志, 2006, 20(3): 2432246.4杨志明. 组织工程基础与临床. 成都: 四川科学技术出版社, 2000:26244.5