Clinical Research on Real-time Quantitative PCR Detection of HPVYUAN Yuan,HUANG He(Liaoning Institute of Traditional Chinese Medicine,Shenyang 110034,China)ABSTRACT:OBJECTIVE to establish a method for the detection of human papilloma virus HPV 23 typing by re-al-time fluorescence quantitative PCR. METHODS DNA HPV was detected by real-time fluorescence quantitativePCR. RESULTS Among 60 patients,19 cases were detected with HPV infection. CONCLUSION The methodhas high sensitivity and specificity for detection of human papilloma virus by real-time fluorescent quantitative PCR,which can provide important clinical guidance for prevention,development and prognosis of cervical cancer.KEY WORDS: Real-time fluorescence quantitative PCR;