Resi dues Anal yti calM ethods forM etam i fopand Its M etabol i tesLU OJ i ng,SH I H ai —yan,PEN G W en—tao,W AN GM i ng—huat D epartm ent fdPesti ci deSci ence,Col l ege ofPl antProtecti on,N a畸tng Agri cul turalU ni versi ty.Key Laboratory(J J M oni tori ngandM anagem ent of CropD i s-ea ses and Pest Insects,M i ni stry ofAgri cul ture,N anj i ng 210095,Chi na)Abst ract :Thehi gh perform ance l i qui d chrom atographi cm ethod W aS devel opedfor the si m ul taneous determ i nati on ofm etam i f opand threem aj orm etabol i tes i n ri ce and envi ronm ent.Resi dues w ere extracted w i th a m i xture ofaeetoni tri l e andw ater,fol l ow edby cl eanupw i th a si l i cagelcol um l l and determ i nedbyH PLC w i th al i near gradi ent el uti onprogramoface—toni tri l e and w at eras the m obi l ephaseand ul travi ol et detecti on at 224 nm .The resul ts show ed that the l i nearrangeof m et·am i fopand i ts m etabol i tes w erefrom0.5m g/t。to20m g/L,w i ththe correl ati on coeffi ci entsbei ng0.999 3 to 0.999 9.Them i ni m umdetectabl e concentrati on of the 4anal ytes i nsoi l ,ri ce straw 。ri cehul l s andunpol i shed grai n sam pl esw ere al l 0.10m g/kgand 0.02 m g/kgi npaddyw atersam pl es,respecti vel y.Averagerecoveri es w ere 78.2%to106.9%,w i ththecoeffi —ci ents of vari ati onrangi ngfrom2.0%to1 1.7%.The resul ts m eettherequi rem entofpesti ci deresi dueanal ysi s.Keyw ords:m etam i fop;ri ce;hi gh perform ance l i qui d chrom atography( H PLC) ;resi due anal ysi s