2015年4月湖北农业科学 Hubei Agricultural Sciences V01.54 No.8 Apr.,2015 土霉素暴露对水培小麦生长及根际微生物的影响 (1.三门峡职业技术学院,河南三门峡472000;2.河南师范大学生命科学学院,河南新乡453002)摘要:采用水培法研究了土霉素(OTC)暴露对小麦(Triticum aestivum Linn.)生长和根际微生物群落结构 的影响。结果表明.在设定的土霉素浓度下,小麦发芽率受抑制程度与处理浓度呈正相关,最大抑制率出 现在处理浓度为100 mg/L时,这40%;土霉素对小麦根长和芽长的抑制效应显著(P<0.05),时间和剂量 效应明显.PCR—DGGE分析结果表明.0.8 mg/L和4.0 mg/L土霉素暴露对根际微生物群落结构影响较 小.浓度在20.0 mg/L以上对微生物群落结构影V向显著,出现新的优势种群。 关键词:土霉素;小麦(丹itiCltm Cle￥tivMm Linn.);微生物群落 中图分类号:￥482.28:S512.1 文献标识码:A 文章编号:0439—8114(2015)08—1832—04 DOhl0.14088/j.cnki.issn0439—8114.2015.08.011 Influence oxytetracyclineExposure RhizosphereMicrobial Community WheatSeedlings HydroponicCulture ZHANG JingIYANG Qing-xian92 (1.Sanmenxia Polytechnic,Sanmenxia 472000,Henan,China; 2.College LifeSciences,Henan Normal University,Xinxiang 453002,Henan,China) Abstract:In study,theeffects wheat(Triticumaestivum Linn.)growth micro—bial community structure rhizospherewere investigated under hydroponic culture conditions.The results showed wheatseeds OTC,theinhibition rate positivecon'elation settingOTC concentrations, highestrate reached 40%whenexposed 100mg/L OTC.The elongation wheatshoots signifi—cantly(P<0.05)inhibited agoodtime dose—responserelationship between OTCconcentration inhibitionrates.PCR—DGGE analysis indicated bacterialcommunity structure signifcantlydifferent wereexposed highlevels OTC(over20.0 mg/L)than wereexposed lowerconcentrations.As OTCincreased,some resistant species appeared. Key words:oxytetracycline;wheat(Triticum aestivum Linn.);microbial community 土霉素作为广谱抗菌素.低廉的价格和显著的 抑菌效果使其成为最为常用的抗菌促生长兽药添 加剂[11。