Exhibit 10.1

FIRST AMENDMENT TO AGREEMENT OF SALE AND PURCHASE

THIS FIRST AMENDMENT TO AGREEMENT OF SALE AND PURCHASE (this “Amendment”) is
made and entered into as of this 14th day of August, 2009 by and between
Greenville Hospital System, a political subdivision organized under the laws of
South Carolina and Board of Trustees of Greenville Hospital System (aka, The
Board of Trustees of The Greenville Hospital System) (collectively, “GHS”),
Greenville Health Corporation (“GHC”), a South Carolina corporation and GHC
Health Resources, Inc. (“GHR”), a South Carolina corporation, all having an
address at 701 Grove Road, Greenville, SC 29605 (collectively, “Seller”), and
HTA — Greenville, LLC, a Delaware limited liability company, having an address
at 16427 N. Scottsdale Road, Suite 440, Scottsdale, Arizona 85254 (“Buyer”).
Seller and Buyer are each individually referred to herein as a “Party” and
collectively as the “Parties”.

R E C I T A L S:

A. Seller and Buyer entered into that certain Agreement of Sale and Purchase as
of July 15, 2009 (the “Agreement of Sale”).

B. Seller and Buyer desire to amend the Agreement of Sale as described herein.

NOW, THEREFORE, in consideration of the terms, conditions and covenants
contained herein, and of other good and valuable consideration, the receipt and
sufficiency of which is hereby acknowledged, Seller and Buyer agree as follows:

1. Greenville Hospital System. The first paragraph of the Agreement of Sale is
hereby amended by deleting the phrase “Greenville Hospital System (“GHS”), a
political subdivision organized under the laws of South Carolina” and hereby
replacing it with the phrase “Greenville Hospital System, a political
subdivision organized under the laws of South Carolina and Board of Trustees of
Greenville Hospital System (aka, The Board of Trustees of The Greenville
Hospital System) (collectively, “GHS”)”. All references in the Agreement of Sale
to GHS shall be deemed to refer to GHS, as such term is amended in this
Amendment, as if such term had such meaning from the Effective Date of the
Agreement of Sale. The Board of Trustees of Greenville Hospital System (aka, The
Board of Trustees of The Greenville Hospital System) hereby acknowledges and
agrees to be bound by the terms, conditions and obligations of the Agreement of
Sale, to the same extent as if it had been an original party thereto and
included as “Seller” from the Effective Date.

2. Greenville Health Corporation. All references in the Agreement of Sale to
“Greenville Health Corporation, Inc.” are hereby deleted and replaced with
“Greenville Health Corporation”.

3. Section 4(a). Section 4(a) of the Agreement of Sale is hereby amended by (i)
deleting “August 14” from the first sentence therein and replacing it with
“August 21”. The Parties agree that for all purposes of the Agreement of Sale,
the Due Diligence Period shall be deemed to expire on August 21.

4. Reaffirmation. Except as modified by this Amendment, Seller and Buyer hereby
reaffirm all terms, covenants and conditions contained in the Agreement of Sale.
The Parties agree that except as modified herein, all other terms of the
Agreement of Sale shall remain in full force and effect.

5. Counterparts. This Amendment may be executed in any number of identical
counterparts, any or all of which may contained the signatures of fewer than all
of the Parties but all of which shall be taken together as a single instrument.

6. Distribution. Counterparts to this Amendment may be executed and delivered by
facsimile or other electronic transmission, and for purposes of this Amendment,
signatures so transmitted shall be deemed to be original signatures.

7. Capitalized Terms. Capitalized terms used in this Amendment and not otherwise
defined herein shall have the meanings ascribed to them in the Agreement of
Sale, unless the context clearly requires or indicates otherwise.

[SIGNATURE PAGE FOLLOWS]

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IN WITNESS WHEREOF, the parties have executed this First Amendment as of the
day, month and year first written above.

    Date: August 14, 2009 SELLER:

GREENVILLE HOSPITAL SYSTEM,
a political subdivision organized under the laws of South Carolina

By: /s/ Susan J. Bickel
Name: Susan J. Bickel
Title: Vice President and CFO

      Date: August 14 2009 BOARD OF TRUSTEES OF GREENVILLE HOSPITAL SYSTEM    
(aka THE BOARD OF TRUSTEES OF THE GREENVILLE     HOSPITAL SYSTEM)    
By: /s/ Susan J. Bickel
   
 
Date: August 14, 2009
a South Carolina corporation  
Name: Susan J. Bickel
Title: Vice President and CFO
GREENVILLE HEALTH CORPORATION,

By: /s/ Susan J. Bickel
   
 
Date: August 14, 2009
a South Carolina corporation  
Name: Susan J. Bickel
Title: Secretary/Treasurer
GHC HEALTH RESOURCES, INC.,

By: /s/ Susan J. Bickel
   
 
Date: August 14, 2009  
Name: Susan J. Bickel
Title: Secretary/Treasurer
BUYER:
   
HTA – GREENVILLE, LLC,
a Delaware limited liability company
By: /s/ Kellie S. Pruitt
   
 

    Name: Kellie S. Pruitt

Title: Chief Accounting Officer

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