Task: Translation
Input (Czech): Biologick² zp sob v²roby kyseliny 7-aminocefalosporanov , 7-ACA, p°i n m  se 1) v p° slu n m  ivn m prost°ed  p stuje kmen Penicillium chrysogenum, produkuj c  isopenicillin N a do  ivn ho prost°ed  se p°id v  jako substr t adip t, a to kyselina adipov , jej  soli nebo jej  estery, asimilovateln  a vyu iteln  pou it²m kmenem Penicillium chrysogenum za vzniku kyseliny adipoyl-6-aminopenicilanov , adipoyl-6-APA, 2) expres  odpov daj c ho genu in situ se uskute n  n sleduj c  p°em ny: a) expanze kruhu adipoyl-6-APA in situ za vzniku kyseliny adipoyl-7-aminodesacetoxycefalosporanov , adipoyl-7-ADCA p soben m enzymu expand zy tak,  e se pou it² kmen P. chrysogenum transformuje k dovou DNA pro enzym expand zu, pro ni  je substr tem adipoyl-6-APA, p°i em  u adipoyl-6-APA, produkovan  uveden²m kmenem dojde in situ k expanzi kruhu a ke vzniku adipoyl-7-ADCA, b) 3-methylov² postrann  °et zec adipoyl-7-ADCA se hydroxyluje in situ za vzniku kyseliny adipoyl-7-aminodeacetylcefalosporanov , adipoyl-\
Output (English): In the present invention there is disclosed a bioprocess for preparing 7-aminocephalosporanic acid (7-ACA) comprising the steps of 1) maintaining in a culture medium capable of sustaining its growth, a strain of Penicillium chrysogenum which produces isopenicillin N and adding to said culture medium an adipate feedstock comprising any one or more of adipic acid, or its salts and esters which are capable of being assimilated and utilized by said strain of Penicillium chrysogenum to produce adipoyl-6-aminopenicillanic acid (adipoyl-6-APA 2), whereby said adipoyl-6-APA is produced; 2) carrying out the following enzymatic conversions by in situ expression of the corresponding gene: a) adipoyl-6-APA is in situ ring-expanded to form adipoyl-7-aminodesacetoxycephalosporanic acid (adipoyl-7-ADCA) by expandase enzyme wherein said strain of P. chrysogenum has been transformed by DNA encoding the activity of the expandase enzyme capable of accepting said adipoyl-6-APA as a substrate, whereupon as