Task: Translation
Input (Czech): Rešení se týká zpusobu detekce genetického polymorfizmu bodové mutace u ovcího kappa-kaseinu polymerázovou retezovou reakcí (PCR) a za použití prístroje typu Light Cycler. Stanovení bodové mutace C ---› T, tedy zámeny nukleotidu, se provádí novými primery a próbami  Primery:  k-casein F: 3' - ATA ATC AAT TTC TgC CAT ACC CAT AT - 5' (25-mer) k-casein R: 5' - TgT ACT gTA ggC TCA gCA CTA gC - 3'        (23-mer)  Próby:  Anchor: 5' - TTC CAC CAA AgA AAg ATC Agg ATA AAA CAg - 3' (30-mer) Senzor[A]: 5' - CCC ACA TTT ATC ATT TAT ggC C- 3'      (22-mer).
Output (English): The present invention relates to method of detecting genetic polymorphism of point mutation in ovine kappa-casein by polymerase chain reaction (PCR) and by making use of an instrument of the Light Cycler type. Determination of the point mutation C ---ø T, i.e. substitution of nucleotides is carried out by new primers and probes. Primers: k-casein F: 3' - ATA ATC AAT TTC TgC CAT ACC CAT AT - 5' (25-mer); k-casein R: 5' - TgT ACT gTA ggC TCA gCA CTA gC - 3' (23-mer). Probes: Anchor: 5' - TTC CAC CAA AgA AAg ATC Agg ATA AAA CAg - 3' (30-mer); Sensor [A]: 5' - CCC ACA TTT ATC ATT TAT ggC C- 3' (22-mer).