Task: Question Generation
Input (English): Context: To examine the direct effect of high glucose levels on primary cultured human retinal capillary endothelial cells (HRCEC).', 'HRCECs were isolated from donated eyes and cultured for 6 days in the media containing 5 or 25 mmol/L glucose. The cell viability was determined by trypan blue exclusion assay and cell cycle analyzed by flow cytometry, with the cell apoptosis assayed by TUNEL method.', 'The cell viability was significantly decreased after exposure to 25 mmol/L glucose, and the number of apoptotic cells determined by flow cytometry and TUNEL was significantly increased in response to high-dose glucose treatment.
Answer: High-dose glucose induces apoptosis in HRCEC, which may contribute to the development of diabetic retinopathy.
Output (English): Does [ High-dose glucose induce human retinal endothelial cell apoptosis ]?