Task: Question Answering
Input (English): Question: Pyruvate carboxylase: 
 Options:  <1> It requires biotin. <2> Use a methyl group activated as a carbon source. <3> It catalyzes the production of acetyl-CoA and carbon dioxide. <4> It is activated in the muscle during the Cori cycle. <5> It is an intermediate of the citric acid cycle.
Output (English): 1