Task: Answer Verification
Input (English): Question: Do hydroxylated estrogens ( 2-OH-E2 AND 4-OH-E2 ) activate cAMP/PKA and ERK1/2 pathways activation in a breast cancer MCF-7 cell line?
Answer: We have shown that E2 hydroxylated metabolites do not activate cAMP/PKA in breast cancer cells and confirm previously published data, which showed a lack of ERK1/2 activation in a breast cancer cell line. The observed reversible action of PD98059 on cell proliferation can be explained by the fact that hydroxylated estrogens, as E2, stimulate secretion of a number of growth factors, which affect MAPK activity, suggested by Lobenhofer et al. (2000).
Output (English): no