Task: Answer Verification
Input (English): Question: Does freezing infective-stage larvae from Anisakis simplex and their produce at -20 degrees C for 24 h prevent the occurrence of autonomic imbalance in rat ileum?
Answer: 1) The gastrointestinal alterations provoked by As are not necessarily associated with a prior infection; and 2) the storage of the seafood at -20 degrees C during 24 h does not prevent the intestinal autonomic imbalance provoked by As, whereas, the prior heating at 60 degrees C for 15 min may completely prevent such process.
Output (English): no