Task: Answer Verification
Input (English): Question: Is additional reverse transcription-polymerase chain reaction of peripheral slices superior to analysis of the central slice in sentinel lymph nodes from melanoma patients?
Answer: Molecular analysis of peripheral slices yielded six additional patients (10%) positive by RT-PCR who were initially negative by standard RT-PCR of the central slice. However, three of these six patients were found to express only MelanA but not tyrosinase. As MelanA expression was also found in 13% of control lymph nodes, positive MelanA expression alone in SLNs of melanoma patients requires cautious interpretation in order to avoid false-positive findings. Thus, additional molecular processing of peripheral slices did not significantly increase the number of patients with RT-PCR-positive SLNs.
Output (English): no