Task: Answer Verification
Input (English): Question: Is global leukocyte DNA methylation altered in euthymic bipolar patients?
Answer: The assay we used, based on restriction by methylation-sensitive/insensitive enzymes followed by a radioactive DNA polymerase reaction was approved to accurately measure global DNA methylation, but has technical limitations i.e. restriction enzymes do not cleave all potential methylation sites in the genome and restriction sites may be altered by mutations or polymorphisms.
Output (English): no