Task: Answer Verification
Input (English): Question: Do baculovirus-mediated gene transfer and recombinant protein expression interfere with insulin dependent phosphorylation of PKB/Akt in human SHSY-5Y and C3A cells?
Answer: Widely-used adenovirus vectors for gene delivery cause a state of insulin unresponsiveness in human SHSY-5Y and C3A cells in culture due to the activation of central protein kinases of the insulin signalling pathway. This phenomenon can be avoided when studying insulin signalling by using recombinant baculovirus as a heterologous viral expression system. In addition, our data may contribute to an understanding of the molecular mechanisms underlying baculovirus infection of human cells.
Output (English): no