表(1)-2根拠Bに基づく農薬環境変化体の調査結果

Pesticide	CASRN	PTPW	Ref.	PTPW	Ref.	PTPW	Ref.
bentazone-sodium, bentazone	25057-89-0	NIA
BPPS	2312-35-8	NIA
captan	133-06-2	NIA
carbam	144-54-7	NIA
carbam-sodium	144-54-7	NIA
cartap	15263-52-2	nereistoxin (1631-58-9)	5
chkropicin	76-06-2	NIA
dazomet	533-74-4	NIA
DCMU	330-54-1	monuron (150-68-5)	6	trans -3-chloroalkyl alcohol (4643-06-5)	7.8
D-D	542-75-6	cis -3-chloroalkyl alcohol (4643-05-4)	7.8	trans -3-chloroalkyl alcohol (4643-06-5)	7.8
DDVP	62-73-7	dimethyl phosphate (813-78-5)	9
DEP	52-68-6	NIA
diazmon	333-41-5	2-isopropyl-6-methyl-4-pyrimidinol (2814-20-2)	10, 11	diethyl phosphate (598-02-7)	11	diazoxon (962-58-3)	11
1-hydroxy isopropyl diazoxon (32588-11-7)	11	1-hydroxy isopropyl diazoxon (29820-16-4)	11
DMTP	950-37-8	O,O-dimethyl S-[2-methoxy-3-1,2-thiadiazolin-5-one] (29856-16-1)	12	O,O-S-trimethyldithiophosphonic acid (2953-29-9)	12	O,O-S-trimethyldithiophosphonic acid (152-20-5)	12
O,O-O'O'-tetramethyl-S-S-methyldithiophosphonic acid (-)	12	2-methoxy-4-methyldithiophosphonic acid (19691-52-2)	12	2-methoxy-4-methyldithiophosphonic acid (-)	12
2-methoxy-4-methyldithiophosphonic acid (-)	12	bis(2-methoxy-1,3,4-thiadiazolin-5-one-4-yl)disulfate (-)	12	1,3,4-oxodiazolidine-2,5-dione (-)	12
ethylthiometon	298-04-4	disulfoton-oxon (126-75-0)	13	disulfoton-sulfoxide (2497-07-6)	13	dithiophosphoric acid-O,S-O-triethylester (2524-09-6)	13
fosfiazate	98886-44-3	NIA
glyphosate, ammonium salt	1071-83-6	(aminomethyl)phosphoric acid (AMPA) (1066-51-9)	14
glyphosate, isopropylamine salt	1071-83-6	NIA
isothiocyanate methyl	556-61-6	NIA
isoxathion	18854-01-8	NIA
malathion	121-75-5	NIA
maneb	12427-38-2	NIA
manzeb	8018-01-7	NIA
MEP	122-14-5	formyl-fentrothion (59417-72-0)	15, 16	3-methylphenol (108-39-4)	15	methyl paraoxon (950-35-6)	16
amino-fentrothion (13306-69-9)	16	O,O-dimethyl O-3-formylphenyl phosphorothioate (-)	16	carbomethoxyfentrooxon (-)	17
3-methyl-4-nitrophenol (2581-34-2)	15, 16, 18, 20-25	S-methylfentrothion (3344-14-7)	15-17, 19, 22-25	methyl parathion (298-00-0)	15-17, 20, 22, 23
3-methyl-4-nitroanisol (5367-32-8)	15, 23	carbomethoxyfentrothion (-)	22	carbomethoxyfentrothion (-)	15-17, 22
tetramethyl pyrophosphate (690-49-3)	19, 22	O,O-dimethyl O-4-methoxyphenyl phosphorothioate (59288-81-2)	23	O,O-S-tri-methyl phosphorothioate (152-20-5)	15, 16
trimethyl phosphate (512-56-1)	17, 19, 22	dentrofentrothion (4829-03-2)	15, 16, 22, 23	fentrooxon (2255-17-6)	15, 16, 18, 20-22
polycarbamate	64440-88-6	NIA
propine	12071-83-9	NIA
pyridaphenthion	119-12-0	NIA
thiophane methyl	23564-05-8	NIA
thiram	137-26-8	NIA
tolcofos methyl	57018-04-9	NIA
TPN	1897-45-6	4-hydroxy-2,5,6-trichloroisophthalonitrile (28343-61-5)	26	1,3-dicarbamoyl-2,4,5,6-tetrachloride (1786-86-3)	26	2,5,6-trichloro-4-methoxyisophthalonitrile (57531-87-0)	26
1-carbamoyl-3-cyano-4-hydroxy-2,5,6-trichlorobenzene (115044-73-0)	26	isophthalonitrile (626-17-5)	26	2,4,5-trichloroisophthalonitrile (23076-59-7)	26
2,5,6-trichloro-4-(methyldithiophosphualonitrile (-)	26	dichloro-1,3-dicyanobenzene (-)	27	chloro-1,3-dicyanobenzene (-)	27
zineb	12122-67-7	NIA
NIA: no information available

調査した32種類の農薬のうち6種類の農薬について22種類のPTPWsの環境モニタリングなどが実施されていた。PTPWsの定性分析を行っている23報中の12報は殺虫剤フェニトロチオン（MEP）を対象とした文献であり、対象農薬が偏っていた。また、多くの論文は、予測したPTPWsの生成を確認する方法で同定がなされるターゲット分析を実施しており、未知物質の同定を実施していた論文は1報 $ ^{5)} $ であった。このため、予測困難なPTPWsは検討されておらず、未同定のPTPWsが多く存在していると考えられる。

## 2 ）変異原性および変異原性物質生成能の測定

113 runの塩素要求量試験、384 runの塩素処理実験、44 runのAmes変異原性試験を行い、562サンプルの832,953個の復帰コロニーを計測して解析した。得られた親農薬およびPTPWsの変異原性を