Curative medicine, Diagnostic products

## Life Sciences

## Development of rapid diagnosis kit with IgM monoclonal antibodies labeled with gold particles

Keyword: Protein engineering of IgM antibody, Prolapse the ready-made ideas for a product development, Rapid and reliable diagnosis kit for a bedside diagnosis

## Organizations Involved

☐ Takeshi Sanekata, Associate Professor, Faculty of Agriculture, Tottori University

Yasuharu Namba, Director, Research & Development, BL Co., Ltd.

## 【Abstract】

High performance immunochromatography using anti-CPV IgM monoclonal antibodies labeled with gold particles for the detection of the canine parvovirus (CPV) was developed in collaboration with the Faculty of Agriculture, Tottori University and BL Co., Ltd., which is the world's first. Tottori University has a licensing agreement with BL Co., Ltd. and commercialization of the kit has been a success.

## [Summary of the technology transfer]

Technological Impact

• Establishment of anti-CPV IgM monoclonal antibodies with high reactivity against CPV

• Establishment of labeling techniques of gold particles to IgM monoclonal antibody

• Safety in testing (closed system)

• Shorted time (within 15 minutes)

• Higher sensitivity compared with available commercial kit (about 50 times)

• Contribution for the proper treatment and prevention of infection

## Market Impact

This diagnosis kit has a very high quality compared with foreign kits, so we are doing marketing research to sale this kit in Japan and foreign countries.

## ● Social Impact

This licensing agreement is the first case for Tottori University since becoming a national university corporation. It was published in Nihon Keizai Shimbun and Nihonkai Shimbun in 11. May, 2006.

## ●Special Features of the Collaboration

We developed a high performance diagnoses kit for CPV using anti CPV IgM monoclonal antibodies labeled with gold particles for the first time in the world. In the future, it is expected that various clinical diagnosis kits will be developed with high reactive IgM monoclonal antibodies.

T. Sanekata

## Project Background

Clinical veterinarians desired an instant and rapid diagnosis kit for infectious diseases. We have techniques for the preparation of highly reactive monoclonal antibodies and gold particle labeling techniques. We collaborated to develop a instant kit.

Y. Namba

## Funding History

Viral extraction kit: Sample was mixed with viral extraction buffer, then filtrated with filter.

1. Selected for collaboration project from BL.Co., Ltd.

## I ntellectual property protection

2.JST: Research for Promoting Technological Seeds

Patent application

「Detection of canine parvovirus」

Publication Number JP2007-57495

Positive band

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Reaction plate: Place one drop of a sample in a well, then let stand for 15 minutes. If the CPV exists in a sample, CPV will react with the gold labeled IgM monoclonal antibodies, anti-CPV antibodies and anti-mouse IgM antibody, then observe a red line with naked eyes.

## Turning point in the Project

ODo not become twisted around someone’s little finger.

OCooperative development with a venture company which has technical capabilities is very desirable

ODo not give up until success.

For more information, contact : Takeshi Sanekata, Faculty of Agriculture, Tottori University, +81-857-31-5679, sanekata@muses.tottori-u.ac.jp