Introduction The developments of the serological test have taken some time to revolute from tiresome manual procedures to simpler techniques, which can allow automation during diagnosis and when reading results. This antigen antibodies detection is now a mandatory laboratory practical test usually performed to determine and identify various disease causative agents such as; viruses, bacteria’s, fungi’s and other parasites capable of penetrating the body and stimulating an immune response such as humoral or cell-mediated immunity which is very specific to the invaded antigen. (1) Samples required for the diagnosis include; body fluids such as blood, urine, and saliva which have the capability of harboring antigens. The detection and identification of the disease causative agents are very important when it comes to treatment administration. The physician may also do some preliminary examinations aided by visual symptoms on the patients before a confirmatory antigen detection diagnosis is done. (1, 2) Old serological techniques Conventional Tube method This is an essential technique precisely used for blood cross-matching tests which should always be performed before any blood transfusion takes place. This technique is also referred to as the spin tube method, basically used for blood compatibility confirmatory test and which requires potentiation with the bovine albumin enzyme together with anti-human globulin (AHG). (1) The technique requires a drop of 5% suspension of antibody screening cell, added to 4 drops of the polyspecific AHG test serum and two drops of LISS-ADDS; all in their appropriate tubes. (1) The tubes are then incubated at 37oC for 15 min then the cells are completely washed three times using normal saline and the supernatant drained out leaving only cellular residual at the bottom of the tube; “the anti-globulin test follows using the DiaClone polyspecific IgG (DiaMed) together with compliment AGH reagents”. (1) After centrifugation for 15 minutes at 3400r/ min, hemolysis or agglutination is observed macroscopically for positive tests, and the negative results are confirmed microscopically. (1) Immunoprecipitation This is another manual method of antibody detection; it is also scientifically referred to as IP (Immunoprecipitation) . (2) During laboratory Immunoprecipitation tests, the patient blood is first drawn and exposed to a specific test antigen which is known (2) and if the patient body had produced antibodies against that test antigen, then the antibodies which are in the blood will bind to all the test antigens present forming antigen-antibody complexes. (2, 3) Notable to mention is that all the antibodies will remain unbound if the antibody specific to the test antigen is absent in the patient blood and this would give a negative result. (2) On the contrary, if the test is positive, the bounded antigen and antibody are thereafter isolated and removed from the sample for analysis using other more advanced lab methods used for specificity confirmation. (2) Presence of the antibody confirms that the patient is sick and should be subjected to immediate treatments; this technique helps diagnose diseases like hepatitis c viru