The study under consideration is Development of Simulated Intestinal Fluids Containing Nutrients as Transport Media in the Caco-2 Cell Culture Model: Assessment of Cell Viability, Monolayer Integrity and Transport of a Poorly Aqueous Soluble Drug and Substrate of Efflux Mechanisms. The research was conducted by a group of scientists: Marianne L. Lind, Jette Jacobsen, Rene Holm and Anette Mullertz. The description of the study has been published in the European Journal of Pharmaceutical Sciences , Volume 3, Issue 2 (2007). As such, it is a peer-reviewed article that, by the very nature of its publication, can be certified original. According to the study by Patel et al. (2006), in most in vitro studies of oral drug permeability, little attempt is made to reproduce the gastrointestinal lumenal environment. The current research is aimed at identifying a transport medium that would have greater physiological relevance in the realm of caco-2 cell culture studies. As the use of physiological conditions in Caco-2 cell transport studies affects the transport of compounds, the general purpose of the research “was to identify a simulated intestinal fluid containing physiological relevant surfactants (TC and PC/lyso-PC) and nutrients compatible with the Caco-2 cell culture model and to examine the impact of the identified SIF on the transport of a poorly aqueous soluble compound and a substrate of efflux mechanisms.” (Lind et al. 2007, p. 262) The present study extends the knowledge on the problem that other authors have reached. For example, Ingels’s and Augustijins’s researches have shown that solutions such as dimethyl sulfoxide (DMSO) or ethanol or solubilised by cyclodextrins or surfactants reduce the physiological relevance of the transport studies, and there is a need to study simulated intestinal fluids by the addition of bile salts, phospholipids and/or fatty acids (Ingels and Augustijns 2003). Many studies have focused on the components of intestinal fluids and intestinal processes (Mansbach et al., 1975; Persson et al., 2006). Experiments on rats and Caco-2 cells conducted by Patel et al. (2006) have shown that using physiological solutions can influence permeability measurements. Studies also illustrated that absorption of lipophilic compounds, cholesterol and carotenoids were dependent on the type of phospholipid present (Hamada et al., 2005). Lyso-PC enhanced the absorption of cholesterol and carotenoids from micelles compared to PC. In the current study, Leibovitz’s L-15 medium (L-15) has been used as the transport solution. It has been identified as the simulated intestinal fluid containing physiological relevant surfactants that are independent of the supply of CO2. By identifying such a transport solution and by examining its impact on the transport of a poorly aqueous soluble compound, the study adds to the existing literature. It further examines its impact on a substrate of efflux mechanisms. We should admit that the authors of the report under analysis always make appropriate references to the previous studies explaining whether the current work is in line with their findings or contradicts them. For example, the authors refer to Martin et al. studies and suggest that their research