Introduction “Hematoxylin and Eosin (H&E) is the most common stain method used in a histopathology lab” (Norman 2009, p.45). Hematoxylin is a solution that stains the cell nucleus to a blue or purple hue. Eosin solution is applied as a counter-stain after (H&E) and acts to stain what hematoxylin did not (Norman 2009). Eosin produces red orange and yellow orange cytoplasm and smooth collagen tissues respectively. For the sake of this practical, there was focus on the human uterus. “This method uses hematoxylin solutions for nuclear staining and eosin solutions for cytoplasm staining” (Cook 2006, p.150). Initially the nuclei are stained with a hematoxylin solution and will stain either blue, dark violet to black. This is then followed by counterstaining with a xanthenes dye for example eosin Y, Eosin B or the erythrosine B. For this kind of process cytoplasm, collagen, keratin and erythrocytes will stain red (Luna 2005, p.401). With this method of staining, one is able to have an overview of a tissue structure, enabling the determination of structures as being normal, inflamed or degenerative changed or pathological (Eglars 2009). A diagnosis can be made based on the results of this method and is used for the following materials like; paraffin sections, frozen sections and clinical- cytological specimens. They may be uterus, urine, sputum, lavages or effusions. In the long run this method gives outstanding results from the combination, coordinated components, easy to apply as it is already developed, produced and tested (Knowles 2001). The main principle behind all the hematoxylin process is that the positively charged metal-hematein complexes bind in an acid milieu to the negatively charged phosphate remains of the cell nucleus-DNA. Hematoxylin is then oxidized in the Gill solutions to hematein with sodium iodate as opposed to the mercury oxidation that was used in the past (Raymond 2008). This one is performed in a controlled way, thus more efficient and less harmful to the surrounding. On the other hand the principle for eosin y which is an acidic colorant that binds mainly to proteins. “The most commonly used is the aqueous eosin y solution in histology” (Walker 2002, p.400). The balancing between nuclear staining with hematoxylin and cytoplasmic staining with eosin is a matter of personal preference. The concentration of the ready-to-use solutions makes it easier for quick obtaining of results (Sudha 2006). Methodology A slide was identified from the previous practical session by careful checking of the pots provided to get one that was to be used. A stop watch was used to get the timings right. All the slides were put together in one holder for the next processes as this was done by a group of students. “The sections of the specimen were deparaffinized by the two changes of xylene which lasted for 5 minutes each” (Walker 2002, p.36). The specimen was then rehydrated in 2 changes of absolute alcohol for 3 minutes each. This was continued in 90% alcohol for 3 minutes and then 70% alcohol for 3 minutes. It was then washed in distilled water briefly and thereafter stained in Giles hematoxylin solution for 6 minutes while the excess was washed