Introduction A vitamin that naturally occurs in many types of food, Vitamin B6 is a collective name for six different vitamers, all with vitamin 6 activity 1 . Though it may be not as commonly known as other vitamins, it still is essential for the proper functioning of human body. In order to maintain the proper percentage of Vitamin B6 in the patient’s body, it is imperative that the dietary allowances of the vitamin should be in direct proportion to the patient’s age; more to the point, pregnant women will need a greater quantity of the vitamin than other patients, which means that the emphasis must be put on fruits (banaa), vegetables, fish (tuna) and cereals. Vitamin B6: Overview The dietary reference intakes (DRIs) established by the FNB are the recommended reference values for the intake of the vitamin 1 . The recommended dietary allowance (RDA) is the most important DRIs 1 . For infants aged between 0 and 6 months, the RDAs are set at 0.1mg for both genders 1 . Between 7 and 12 months, infants should be given 0.3mg while those aged 1 to 3 years should be given 0.5 mg 1 . For children aged 4 to 8 and 9 to 13, the RDA for both genders are 0.6mg and 1.0 mg respectively 1 . Between 14 and 18 years, the RDAs for males is 1.3 while that for females is 1.2 mg. Between 19 and 50 years, both genders’ RDA is 1.3mg while that of people aged 50 and above is 1.7 mg (males) and 1.5 mg (females) 1 . Pregnant and lactating females aged between 14 and 50, the RDA is 1.9 mg and 2.0 mg respectively 1 . This biosynthetic route is well defined in E. coli. In this process, the substrate 3-hydroxyl-1-aminoacetone phosphate undergoes a four step synthetic pathway from D-erythrose 4-phosphate 2 . In the first step, the compound is oxidated to D-erythronate 4-phosphate by its respective dehydrogenase enzyme (GapB), which uses NAD redox factor 2 . In the second step, the D-erythronoate 4-phosphte undergoes a process of oxidation under the action of its respective enzyme PdxB and the NAD cofactor to produce (3R)-3-hydroxy-2-oxo-4-phosphonooxybutanoate 2 . Then, the enzyme PdxF catalyzes the PLP-dependent transmission from the product of the second oxidation to glutamate, which results into the formation of 4-hydroxy-L-threonine phosphate (HTP) 2 . Then, HTP undergoes an oxidative decarboxylation under the influence of enzyme PdxA to form 3-hydroxy-1-aminocetne phosphate. It is believed that the process takes place in two steps 2 . First, alcohol undergoes a nicotinamide-dependent oxidation at the α-position. Secondly, the resulting α-ketoacid is decarboxylated to form the 3-hydroxy-1-aminocetne phosphate 2 . Then, the unstable 3-hydroxy-1-aminocetne phosphate is changed to PNP by PNP synthase. The enzyme PNP oxidase oxidizes PNP to form PLP, the active form of vitamin B 2 . In this pathway, glutamine is first hydrolyzed into ammonia under the catalytic action of Pdx2 using the Glu-His-Cys triad of catalysis. The formed ammonia diffuses to the active site of Pdx1 through the hydrophobic channel 2 . Here, PLP is synthesized from glyceraldehyde 3-phosphate and D-ribose 5-phosphate, ending the process. Two pathways, A and B, are involved 3 . Pathway A has 8 steps for degrading pyridoxine. First, pyridoxine is oxidized to pyridoxal by enzyme pyridoxine 4’-oxid