What is GeneBass?
GeneBass is a CRISPR-Cas9 screening platform for the high-throughput functional analysis of genes in mammalian cells. The GeneBass platform allows researchers to systematically activate or repress genes of interest and study their effects on cellular processes such as cell proliferation, differentiation, and survival. The system uses a pooled lentiviral library of guide RNAs targeting every gene in the human or mouse genome, which can be introduced into cells using viral transduction. The guide RNAs are designed to target specific regions of the genome adjacent to the transcription start sites of each gene, allowing for specific and efficient gene activation or repression. GeneBass experiments typically involve the transduction of large numbers of cells, followed by selection or sorting of cells based on specific phenotypes of interest. High-throughput sequencing of the guide RNA library before and after selection can be used to identify genes that are essential for specific cellular processes or that are involved in disease pathways.