Domain: Healthcare — Pathology
Target concept: Calcification (Atherosclerotic calcification)

Video generation prompt: An old atherosclerotic plaque tissue specimen is placed on the laboratory bench. The lesion's internal structure is observed under the microscope.

Evaluation guide:
(i) Target concept: Visualization of dystrophic calcification in an aged atherosclerotic plaque via light microscopy.
Minimum mechanism: Specimen must be formalin-fixed paraffin-embedded (FFPE) tissue section stained with hematoxylin and eosin (H&E); calcific deposits must be optically dense, electron-dense, and appear as sharply demarcated, irregular, basophilic (blue-purple) or acellular eosinophilic (pink-white) foci depending on stain intensity and section thickness, not as smooth, crystalline, or metallic structures.
(ii)Storyline: [Frame 0–2] Bench is visible; specimen is placed centrally with no motion blur. [Frame 3–4] Microscope is brought into frame and locked in fixed position adjacent to specimen, objective vertically aligned. [Frame 5–10] Stable ocular feed appears and remains centered, showing progressively resolved microscopic field: first general tissue outline → then fibrous architecture → finally distinct, embedded, irregular calcific deposits with high contrast against stroma. Perspective is fixed (no camera orbit); ocular feed occupies consistent region of frame; all transitions are continuous and causally linked (placement → alignment → viewing → resolution).

Manual:
Experimental Procedure
1. Place fixed atherosclerotic plaque tissue specimen on clean laboratory bench.
2. Position upright microscope with objective lens directly above specimen.
3. View specimen through microscope eyepiece or display feed.
Observation of Experimental Phenomena
Microscopic field reveals solid, heterogeneous plaque tissue with irregular, densely basophilic (deep blue-purple), granular calcific deposits — occasionally paler eosinophilic (pink-white) where less densely mineralized — embedded within fibrous stroma; no necrosis, hemorrhage, or acute inflammation.
Summary of Experimental Principles
Atherosclerotic calcification is dystrophic mineralization occurring in aged, fibrotic plaques; deposits are composed of hydroxyapatite crystals and manifest histologically as dense, irregular, basophilic (occasionally pale eosinophilic) laminated foci within collagenous matrix on H&E-stained sections.

Prompt Grounding rubric:
level_1: No laboratory bench is shown, no plaque tissue specimen is visible, no microscope appears, or no observation action is depicted at all; none of the prompt's required setup is established.
level_2: The bench is shown but is empty or holds an unrelated object, or a generic (clearly non-plaque, unlabeled) tissue sample appears instead, or the microscope is present but not oriented toward the specimen at all.
level_3: A tissue specimen is placed on the laboratory bench, a microscope is present in the same scene positioned near the specimen, and the framing implies an intended observation (an eyepiece is in view, or the specimen sits beneath the objective), but no microscopic field or viewfield is actually shown resolving the tissue's internal structure.
level_4: The specimen is placed on the bench, the microscope's objective is aligned directly above it, and a viewfield or ocular feed appears showing some resolved tissue texture, but the field shown does not progress from a general outline into a clearly resolved, high-magnification tissue view (it stays blurry, or stops at low magnification).
level_5: The specimen is placed on the bench, the microscope is correctly aligned over it, and the ocular view or monitor feed shows a progressively sharpening field (first a general tissue outline, then fibrous architecture, then a sharply focused high-magnification view), with the whole sequence (placement, alignment, viewing, resolution) shown in clear causal order and no step skipped or reordered.

Scientific and Causal Correctness rubric:
level_1: No calcification-related feature appears in the tissue; the field looks uniformly soft and homogeneous with no mineral-associated color or texture contrast at all.
level_2: The tissue shows an implausible state (liquid, dissolving, or actively bleeding), or the calcific deposits appear as smooth, uniform, sphere-like or metallic-looking structures rather than irregular basophilic clumps, inconsistent with the granular, dystrophic calcification expected in an aged plaque.
level_3: The tissue is solid with visible fibrous architecture, and deposits consistent with calcification (irregular, densely basophilic blue-purple, or pale eosinophilic pink-white patches) are present, but they appear isolated or superficially sitting on the tissue rather than embedded within the fibrous stroma, or their shape looks too regular/rounded for dystrophic calcification.
level_4: All conditions for score 3 are met, and additionally: the calcific deposits are irregular, clustered, and clearly embedded within the fibrous matrix rather than floating free; there is a visible color/density contrast between calcified and non-calcified regions, but a slight artifact (e.g., an odd halo or edge glow around the deposits) is present.
level_5: The deposits are irregular, densely basophilic (or, where lightly mineralized, pale eosinophilic), fragmented, and fully integrated into the fibrous stroma with no halo, no blur, and no artificial sheen around them; the contrast between calcified foci and the surrounding eosinophilic collagenous matrix reads clearly and naturally, with no staining artifact, air bubble, or optical distortion visible anywhere in the field.

Spatiotemporal Consistency rubric:
level_1: The specimen's position on the bench changes abruptly between frames with no motion carrying it there; the microscope's position or angle jumps without any adjustment gesture shown; the bench surface or specimen visibly fractures, warps, or scales/rotates discontinuously between frames; or the scene's overall brightness swings sharply from bright to dark and back within a couple of frames.
level_2: The specimen shifts slightly on the bench without any visible contact causing it; the microscope's objective changes angle without a matching mechanical motion (its arm doesn't visibly move); the ocular view blinks on and off rather than fading in cleanly once established.
level_3: The specimen, microscope, and bench keep a consistent spatial relationship to each other throughout, and the ocular view, once it appears, stays visible continuously, but there is a brief, minor unsteadiness in the ocular feed or in a reflection on the bench surface (a slight flicker or wobble) that doesn't rise to a structural break.
level_4: No abrupt jump, scaling, or rotation occurs anywhere; the specimen stays adjacent to the microscope base with the objective aligned vertically above it once the microscope is brought into position; the scene's brightness and color stay the same from first frame to last, but the ocular view shows one brief instance of misalignment (shifting noticeably off-center for a moment) or goes slightly soft and refocuses once.
level_5: The specimen stays rigidly fixed in place on the bench once placed; the microscope is brought into frame with smooth, continuous motion and locked into position by the time the ocular feed appears, after which the microscope body and objective hold an identical pose for the remainder of the sequence; the ocular view, once present, stays continuously centered and in focus without ever drifting off-center or blurring; the bench surface and any reflections on it stay visually stable and coherent throughout.