Domain: Healthcare — Medicine
Target concept: Compatibility Check of Medications (pH Compatibility)

Video generation prompt: A transparent test tube containing 1 mL of epinephrine hydrochloride injection was placed on the table. Subsequently, 1 mL of sodium bicarbonate injection was added to the test tube. After thorough mixing, the changes in the appearance of the mixed solution were observed.

Evaluation guide:
(i) Target concept: pH-induced oxidative degradation of epinephrine causing a visible color change (progressive pink-to-brown discoloration), a classic IV drug incompatibility.
Minimum mechanism: Epinephrine hydrochloride injection is acidic and pale, faintly straw-colored when fresh; sodium bicarbonate raises the local pH well above epinephrine's stability range, promoting auto-oxidation of its catechol structure to adrenochrome and further oxidation products, visible as a progressive shift from the original pale color toward pink and then brown, without requiring any precipitate to be visible.
(ii) Storyline: [Frame 0–2] Static shot: transparent test tube centered, containing epinephrine hydrochloride injection (pale straw-yellow clear liquid) on a plain table. [Frame 3–4] Syringe/applicator enters frame, delivers colorless sodium bicarbonate solution into the tube; meniscus rises visibly. [Frame 5–7] Tube is swirled/inverted continuously; liquid rotates cohesively, no splashing. [Frame 8–10] Motion stops; camera holds steady on tube; the solution's hue visibly deepens, shifting from its starting pale color toward pink and then toward brown over the observation window; the solution stays clear/translucent (no white cloud) as the color changes. Perspective: fixed frontal view, shallow depth-of-field focusing on tube mid-section; background neutral/uncluttered; lighting even and diffused so the color shift is unambiguous.

Manual:
Experimental Procedure
1. Place a transparent test tube containing 1 mL of pale, straw-yellow epinephrine hydrochloride injection on a table.
2. Add 1 mL of colorless sodium bicarbonate injection into the test tube.
3. Mix thoroughly by swirling or gentle inversion.
4. Observe appearance change.
Observation of Experimental Phenomena
The solution's color progressively deepens from its initial pale yellow toward pink and then toward brown over the observation period; the liquid stays clear/translucent throughout (no precipitate, no cloudiness); no gas evolution.
Summary of Experimental Principles
Epinephrine hydrochloride is acidic (pH ~3–4); sodium bicarbonate is alkaline (pH ~8.3). Mixing raises the pH above the range in which epinephrine is stable, promoting oxidative degradation of its catechol structure to adrenochrome and further oxidation products — visible as a characteristic pink-to-brown color change, the classic sign of this drug incompatibility.

Prompt Grounding rubric:
level_1: No test tube appears; or the epinephrine hydrochloride injection is never shown; or the sodium bicarbonate injection is never shown; or the tube is never placed on the table; or nothing is ever added to it; or no mixing action occurs; or there's no moment where the solution is observed afterward.
level_2: A test tube with some liquid is shown, but epinephrine hydrochloride and sodium bicarbonate are not both shown as distinct agents (e.g. both liquids look identical before mixing, or only one of the two is ever visibly added); or the actions happen out of order (e.g. mixing shown before the second liquid is added); or the tube is observed before mixing has occurred.
level_3: The test tube sits on the table; the epinephrine hydrochloride injection is visibly present inside it as a pale straw-yellow liquid; the sodium bicarbonate injection is shown separately as a colorless liquid (in its own syringe or container) before being added; the sodium bicarbonate is added into the tube; a mixing action (swirl or inversion) follows; the solution is then observed; all in the correct order.
level_4: Everything required at the 3-point level is shown, and additionally: the two liquids are clearly distinguishable by color before mixing (pale straw-yellow vs. colorless); the test tube is transparent enough, and free of labels/glare, that its contents stay visible throughout mixing and observation.
level_5: Everything required at the 4-point level is shown, and additionally: the tube never moves from its spot on the table; no unrelated object enters or leaves the frame; the syringe or dropper delivering the sodium bicarbonate is clearly seen depositing liquid into the tube itself (not near it or on the outside); the mixing motion visibly reaches the entire liquid volume; and the camera holds a clear, unobstructed frontal view of the tube's contents for at least a second after mixing stops.

Scientific and Causal Correctness rubric:
level_1: No visible change occurs after mixing (the solution still looks exactly as pale as at the start); or the color shifts to something implausible for this reaction (e.g. blue, green, or purple); or gas bubbles/fizzing appear, which this pH shift does not produce.
level_2: Some color change occurs, but it goes the wrong direction or is implausible: the liquid turns visibly cloudy/opaque with a solid precipitate rather than staying a clear/translucent solution; or the color jumps straight to dark brown/black immediately upon mixing rather than progressing; or the color changes and then fully reverts to the original pale color before observation ends.
level_3: After mixing, the solution's color visibly deepens from its initial pale straw-yellow toward pink, consistent with early-stage oxidative degradation of epinephrine at elevated pH; the liquid remains clear/translucent (no precipitate, no persistent cloudiness); there is no gas evolution.
level_4: All of the above holds, and in addition the color continues to progress beyond pink toward a brownish hue within the observation window (rather than stalling at a single shade); the color change is uniform through the liquid rather than confined to one layer or streak; the solution stays translucent throughout, with no settling solid appearing at the bottom.
level_5: All of the above holds, and in addition: the color change begins only after mixing is essentially complete (not while the sodium bicarbonate is still being added or only partially blended in); the progression from pale yellow through pink toward brown is smooth and one-directional (it never lightens back toward the starting color); the solution shows no precipitate or turbidity at any point, so the only reaction sign visible is the pigment shift itself.

Spatiotemporal Consistency rubric:
level_1: The test tube's position or appearance changes abruptly between shots with no visible cause (e.g. it jumps to a different spot on the table, or its shape changes); the liquid inside jumps to a different level or color instantly rather than changing gradually; the scene's overall brightness swings from bright to dark (or the reverse) partway through with no explanation; the tube's shape stops looking like a cylinder (e.g., it briefly looks conical).
level_2: The tube stays roughly in place but tips or rotates more than about 15 degrees for no physical reason; the swirling/mixing motion stops and restarts abruptly two or more times; the liquid surface stays perfectly flat and rigid during mixing instead of showing any ripple or meniscus movement; a hand or tool's shadow appears or disappears in a way that doesn't match when that hand or tool is actually in view.
level_3: The test tube stays in the same spot on the table with no abrupt cuts; the mixing motion is unbroken and moves in one coherent direction (consistent swirl or inversion path); the liquid surface behaves plausibly while mixing (the meniscus shifts, small ripples appear); shadows and highlights stay on the same side of the tube throughout, matching a light source that itself hasn't moved.
level_4: Everything required at the 3-point level holds, and additionally: the table and background do not shift relative to the tube as the camera or objects move; there's no flicker or strobing between frames; the hand/syringe motion and the liquid's response to it are properly synced (e.g., the syringe is fully in frame before liquid is shown entering the tube); the liquid volume stays constant, with no sign of spilling or sudden evaporation.
level_5: Everything required at the 4-point level holds, and additionally: any background motion relative to the tube looks physically proportionate (closer objects appear to move more than farther ones); the liquid keeps a faint residual swirl for a moment after active mixing stops, as real liquid would; shadow edges stay soft and consistent with diffuse lighting; straight edges in the scene (e.g. the table edge) stay straight all the way to the frame's border.