Domain: Healthcare — Pathology
Target concept: Dysplasia (collagenous degeneration)

Video generation prompt: A tissue repair area specimen is placed on the experimental table, and matrix changes are observed under the optical microscope.

Evaluation guide:
(i) Target concept: Microscopic visualization of collagenous degeneration, a connective-tissue matrix injury pattern in a tissue repair zone, marked by fragmentation, disorganization, and hyalinization of collagen fibers in the stroma.
Minimum mechanism: The specimen must be a formalin-fixed, paraffin-embedded, H&E-stained tissue section; the optical microscope must be configured for brightfield transmission illumination at 10×–20× magnification; collagenous degeneration must manifest as irreversible structural derangement, not reversible edema or acute inflammation.
(ii)Storyline: Phase 1 (0–2s): Static wide shot. Tissue specimen centered on clean experimental table, labeled container or scalpel nearby. Phase 2 (2–4s): Hand places specimen onto microscope stage with precise alignment; microscope remains stationary, same fixed viewpoint. Phase 3 (4–6s): Camera cuts to simulated eyepiece POV. Field fills with sharply focused tissue section; stroma occupies central 60% of view. Phase 4 (6–8s): Stable dwell on stromal region. Collagen architecture visibly disrupted: fragmented, non-parallel, hyalinized zones coexist with residual fibrillar areas. Phase 5 (8–10s): No zoom/pan; consistent focus and illumination; no occlusion; final frame holds full diagnostic matrix morphology for unambiguous identification.

Manual:
Experimental Procedure
1. Place a tissue repair specimen exhibiting collagenous degeneration on the experimental table.
2. Position the specimen on the optical microscope stage.
3. Observe the stromal matrix through the eyepiece.
Observation of Experimental Phenomena
Under the optical microscope, collagen fibers in the stromal matrix appear fragmented, irregularly thickened, and partially replaced by homogeneous, glassy (hyalinized) areas—loss of normal parallel fibrillar architecture.
Summary of Experimental Principles
Collagenous degeneration is characterized by pathological matrix remodeling: collagen synthesis becomes disordered, fibers undergo fragmentation and hyalinization, leading to loss of tensile integrity and altered optical properties in tissue repair zones.

Prompt Grounding rubric:
level_1: No tissue specimen appears on the experimental table, no optical microscope is present in the scene, and no eyepiece or stage view of any kind appears; there is no visual evidence of a microscopy setup being assembled.
level_2: A tissue specimen sits on the table and an optical microscope is visible, but the specimen is never moved onto the microscope stage, or no eyepiece view is ever shown, or the specimen and the microscope appear in separate, unconnected shots with no shared placement action linking them.
level_3: The specimen is shown moving from the table onto the microscope stage in the same setting, and an eyepiece view opens, but that view shows a blank field, an unrelated object, or an out-of-focus/non-tissue image rather than a genuine histological section.
level_4: The specimen moves from the table onto the microscope stage within the same continuous setup, and the eyepiece view opens on an in-focus histological image showing recognizable tissue structure (cells and surrounding matrix), but the framing includes a stray element (such as a visible finger, dust speck, or lens edge), or the specimen sits slightly off-center on the stage.
level_5: The specimen moves from the table onto the microscope stage within the same continuous setup with correct alignment, and the eyepiece view opens on a cleanly framed, sharply focused histological section centered on the stroma, with no stray objects, occlusion, or misalignment anywhere in the sequence: full setup fidelity from tabletop to final eyepiece framing, regardless of what specific collagen pattern the tissue shows.

Scientific and Causal Correctness rubric:
level_1: The eyepiece view shows liquid, metal, printed text, an abstract pattern, or other non-biological material; or it shows connective tissue with uniform, parallel, intact collagen fibers and no stromal disruption at all.
level_2: The eyepiece view shows biological tissue but no stromal component is visible (for example, only epithelial cells with no underlying matrix), or it shows necrosis, active inflammation, or hemorrhage instead of any collagen change.
level_3: The eyepiece view shows a tissue section with stroma present and collagen fibers visible, but those fibers remain uniformly eosinophilic (pink-staining), straight, and continuous, with no fragmentation, waviness, hyalinization, or loss of the normal fibrillar pattern.
level_4: The stromal matrix shows collagen fibers with at least one degenerative feature: fragmented segments, OR irregular fiber thickness, OR patchy glassy (hyalinized) areas, but not multiple features occurring together, or the view includes a confounding artifact (e.g., an air bubble overlapping the stroma, or a stain precipitate that mimics fiber breakup).
level_5: The stromal matrix shows collagen fibers exhibiting all three hallmark features of collagenous degeneration together: (1) loss of normal fibrillar organization (fibers are non-parallel, splayed, or disoriented); (2) fragmentation (fibers appear as short, broken, discontinuous segments rather than continuous strands); and (3) hyalinization (glassy, homogeneous, pale-staining regions replace normal fibrillar texture); with no artifacts or staining errors that would confuse the interpretation.

Spatiotemporal Consistency rubric:
level_1: The specimen appears already on the microscope stage with no visible motion from the table (a hard cut skipping the placement step), or the lighting changes abruptly between the tabletop shot and the stage shot (e.g., bright even light on the table then sudden dim or side-lit illumination on the stage), or the specimen's size or orientation changes unnaturally between the table and stage shots, or the eyepiece cut (per the storyline) lands on a jarring double-image, duplicated frame, or glitch instead of landing cleanly on the in-focus stromal view.
level_2: The transfer of the specimen from table to stage happens off-screen and the specimen simply reappears already seated on the stage; the eyepiece view opens already mid-zoom with the framing still settling; the labeled container or scalpel on the table shifts position or disappears between the wide shot and the placement shot; or the shadow the specimen casts on the tabletop points a different way after the hand approaches than before, with no matching change in the light source.
level_3: The specimen is shown moving from the table onto the stage via visible hand motion, and the eyepiece view opens via a single clean camera cut to the simulated eyepiece POV, as the storyline calls for; the direction and brightness of light on the specimen and tabletop match between the wide shot and the placement shot, and no object flickers, duplicates, or changes shape, but the eyepiece framing drifts slightly as it settles after the cut, or a fingertip briefly crosses the edge of the frame for under a second before clearing.
level_4: The sequence's table-to-stage transfer runs as one continuous, physically plausible motion, and the eyepiece view then opens via a single clean camera cut to the simulated eyepiece POV, as the storyline prescribes; the specimen's scale, orientation, and position change only as much as the hand motion and stage placement would produce, the eyepiece framing stays centered on the stroma at a constant magnification once settled, and the brightness and focus of the stromal view do not flicker or pulse.
level_5: The specimen's path from table to stage is a single, physically continuous motion with no teleport or unexplained repositioning; the eyepiece view then opens through a single, clean camera cut to the simulated eyepiece POV exactly as the storyline prescribes, landing immediately on a sharp, centered, stable stromal view with no digital-overlay artifact, duplicated frame, or glitch at the cut point; the specimen, container, and any tool on the table keep the same relative positions and perspective throughout; and the final stromal close-up holds steady with no parallax jump, no duplicated frame, and no unexplained change in framing or magnification.