Domain: Healthcare — Nutrition and Food Hygiene
Target concept: Aflatoxin Testing

Video generation prompt: Ten milliliters of peanut extract containing aflatoxin B1 were placed in a clear test tube. The tube was carried into a darkened room, where a long-wave (365 nm) ultraviolet lamp was switched on and aimed directly at the tube from the side, with nothing placed between the lamp and the tube.

Evaluation guide:
(i) Target concept: Native photoluminescence of aflatoxin B1 -- a photophysical excitation/emission event, not a chemical reaction. Aflatoxin B1's fused difurocoumarin ring system strongly absorbs long-wave (365 nm) UV light and re-emits part of that energy as visible blue fluorescence; this is the same property that gives the 'B' (blue-fluorescing) aflatoxins their name, as opposed to the green-fluorescing 'G' series.
Minimum mechanism: Aflatoxin B1 must be present in the peanut extract; ambient visible light must be minimized (the room darkened) because the fluorescence is comparatively faint and is washed out by room light; a long-wave (365 nm) UV lamp must be switched on and aimed directly at the tube with nothing opaque between the lamp and the tube; the glow appears essentially as soon as the UV light reaches the sample and disappears as soon as the lamp is switched off or its beam is blocked -- no reagent, precipitate, incubation period, or multi-minute wait is required for it to appear.
(ii) Storyline: [Frame 1] Static shot of empty test tube -> [Frame 2] Steady pour of 10 mL pale yellow-brown peanut extract into the tube (volume visually ~50-65% tube height) -> [Frame 3] Tube capped and carried into a room where a UV lamp is set up nearby, room lights already dim or being switched off -> [Frame 4] Room reaches a fully darkened state with only the (still-off) UV lamp visible -> [Frame 5] UV lamp switches on and is aimed directly at the tube; the extract begins glowing a distinct blue almost immediately, with the tube remaining fully visible to the camera the whole time -> [Frame 6] Continuous static shot holding on the glowing tube for several seconds, tube and lamp both fully in frame -- all transitions occur from a fixed frontal viewpoint with no camera repositioning, no cutaways, no overlays.

Manual:
Experimental Procedure
1. Place 10 mL peanut extract into a clear test tube.
2. Carry the capped tube into a darkened room where a long-wave (365 nm) UV lamp is set up.
3. Switch on the UV lamp and aim it directly at the tube, with nothing opaque placed between the lamp and the tube.
4. Observe the tube under continuous UV illumination.
Observation of Experimental Phenomena
Under ordinary room light, the peanut extract is pale yellow to light brown with no glow. As soon as the UV lamp is switched on and aimed at the tube in the darkened room, the extract emits a distinct blue fluorescence that remains visible for as long as the lamp illuminates it, and stops the instant the lamp is switched off or its beam is blocked -- no precipitate, no phase separation, no color change under ordinary light.
Summary of Experimental Principles
Aflatoxin B1's fused difurocoumarin ring system absorbs long-wave UV light (365 nm) and re-emits part of that energy as visible blue fluorescence. This native blue emission -- the same property that gives the 'B' aflatoxins (B1, B2) their name, as distinct from the green-fluorescing 'G' aflatoxins (G1, G2) -- is used here as a rapid visual indicator of aflatoxin B1 in the extract.

Prompt Grounding rubric:
level_1: No test tube is present, no peanut extract liquid is shown, the room is never shown darkened, and no UV lamp is shown; none of the four core setup elements (test tube, peanut extract, a darkened room, a UV lamp) appear anywhere in the video.
level_2: At least one core setup element appears (for example only the test tube, or only a lamp), but the full setup is never shown together: the 10 mL peanut extract is never poured into the tube, the room is never shown dark, and no UV lamp is ever shown aimed at the tube or switched on.
level_3: All four core elements are present -- a clear test tube, pale yellow-brown peanut extract filling roughly half the tube, a visibly darkened room, and a UV lamp -- and the extract is poured into the tube before the room is darkened, but the sequence has a gap: for example the UV lamp is never shown switching on, or the tube's size or shape changes between the pouring shot and the later shot.
level_4: All four core elements are present and correctly sequenced: the extract is poured into the tube first, the tube is capped and carried into the darkened room, and the UV lamp is shown switching on and aimed at the tube; only non-core flaws remain, such as the lamp housing looking generic, ordinary room light lingering faintly in the background, or a slight camera-angle shift between the pouring shot and the later shot.
level_5: All four core elements are present, unambiguous, and correctly proportioned: the test tube stands upright and stable; the peanut extract fills roughly half to two-thirds of the tube and reads as pale yellow to light brown; the room is shown fully darkened except for the UV lamp; the UV lamp is clearly visible, switched on, and aimed directly at the tube with the tube remaining fully visible to the camera throughout; framing stays fixed and continuous throughout with no cuts, no zooms, and no inserted shots.

Scientific and Causal Correctness rubric:
level_1: No fluorescence or glow appears at any point under the UV lamp; the extract's appearance with the lamp on looks identical to its appearance under ordinary light; or the liquid instead shows an ordinary-light color change (for example turning green, brown, or clear) rather than glowing only when illuminated by UV -- showing no link to aflatoxin B1's actual light-emitting behavior.
level_2: Some visual change appears, but it contradicts aflatoxin B1's known fluorescence: the extract glows red, white, or yellow-green rather than blue; the glow is already visible before the UV lamp is switched on or while the room is still lit; or the glow only appears after several minutes of waiting, as though a slow chemical reaction were producing it rather than an immediate light-driven emission.
level_3: The extract shows no glow under ordinary light and, once the UV lamp is on and the room is dark, emits a distinct blue glow that appears essentially as soon as the lamp turns on, roughly uniform across the liquid -- but one science-relevant ambiguity remains, such as the glow reading closer to green-tinted cyan than blue, or the glow appearing to linger for several seconds after the lamp is shown switching off.
level_4: Baseline appearance under ordinary light is pale yellow to light brown with no glow; the instant the UV lamp illuminates the tube, the extract emits a clearly blue fluorescence, uniform through the liquid column, with no precipitate, layering, or gas evolution, and the glow is shown depending on both the UV lamp being on and the surroundings being dark; only one minor issue remains, such as the blue reading very slightly desaturated or a faint bright rim at the meniscus that is not itself mistaken for a separate color change.
level_5: Under ordinary light the extract shows only its pale yellow-brown color with no glow; once the room is dark and the UV lamp is on, the entire liquid column immediately fluoresces a clear, saturated blue with no delay, no secondary hue such as green or red, and no non-photophysical artifacts such as bubbling, precipitate, or phase separation; the blue fluorescence is shown strictly coincident with the lamp being on and the surroundings being dark, matching aflatoxin B1's documented blue emission under 365 nm UV excitation.

Spatiotemporal Consistency rubric:
level_1: The test tube's position or size jumps abruptly from one frame to the next with no pouring, carrying, or placing motion shown in between; the peanut extract's volume changes with no visible stream of liquid explaining the change; the UV lamp appears switched from off to on (or on to off) with no switch or button action shown; or the room switches from lit to fully dark in one frame with no light being turned off or dimmed shown in between.
level_2: Major continuity breaks remain: the extract's pour starts and stops with no visible stream forming the rising fill level; the tube snaps from tilted to upright with no righting motion; the room's lights switch off in a single instantaneous jump with no visible switch-flipping or fading motion; or the scene switches from lit to fully dark in one frame with no dimming shown beforehand.
level_3: The pour, the tube being carried into the room, and the lamp switch-on are each shown as continuous physical actions -- a visible liquid stream fills the tube, the tube is visibly carried from one location to the next, the lamp's switch is visibly pressed or toggled -- but a minor flaw remains, such as one frame where the tube's cast shadow does not match the lamp's shown position, or a brief stutter in the lamp's switching motion.
level_4: All actions are physically coherent: the pour follows a smooth, gravity-consistent stream; the tube is carried and set down without any snap in position; the room's darkening is shown as a continuous fade or a visible light switch-off rather than an instant cut; the UV lamp's on transition is shown via a visible switch or button press rather than an instant cut; only one small flaw remains, such as a single frame of slight focus softness while the lamp is being aimed at the tube.
level_5: The entire sequence is free of spatiotemporal breaks: the tube's position, tilt, and size change only through visibly continuous motion during pouring, carrying, and placing; the room's darkening and the UV lamp's switch-on are each shown as a complete, uninterrupted action; once the lamp is on and the room is dark, the tube and its fluorescence stay steady and unchanging in position, framing, and brightness for the entire held shot, with no jump cuts, no flicker, and no unexplained shift in the lamp's position or angle.