Domain: Healthcare — Pathology
Target concept: Tumor (Malignant Tumor Cell Morphology)

Video generation prompt: A malignant tumor tissue section is placed on the experimental table, and cell morphology is observed under the optical microscope.

Evaluation guide:
(i) Target concept: Optical microscopic visualization of diagnostic histopathologic features in a surgically resected malignant tumor tissue section.
Minimum mechanism: Light transmission through a thin, H&E-stained formalin-fixed paraffin-embedded (FFPE) tissue section mounted on glass, magnified via compound optical microscope with objective and ocular lenses; malignancy assessment relies solely on morphology visible at ×400 magnification.
(ii)Storyline: [Frame 1] Static wide shot: experimental table with tissue section centered; microscope placed stably beside it. → [Frame 2–3] Cut or transition to microscope eyepiece POV: view snaps into focus revealing histological field. → [Frame 4–8] Sustained in-focus view: cellular field fills frame; malignant features emerge sequentially as eye accommodates (first nuclear size variation, then irregular contours, then mitoses). → [Frame 9–10] Stable composition holds: no camera motion, no object repositioning, no lighting shift. Perspective is strictly observer-eye-level; no overhead drone view, no macro close-up of unmounted tissue, no digital interface overlay.

Manual:
Experimental Procedure
1. Place malignant tumor tissue section on experimental table.
2. Position optical microscope adjacent to section, aligned for viewing.
3. Observe cell morphology through microscope eyepiece.
Observation of Experimental Phenomena
Microscope view shows densely packed cells with enlarged, irregular, hyperchromatic nuclei; high nuclear-cytoplasmic ratio; loss of cellular polarity; abnormal mitotic figures visible.
Summary of Experimental Principles
Malignant tumor cells exhibit characteristic morphologic abnormalities: nuclear pleomorphism, hyperchromasia, increased nuclear-cytoplasmic ratio, disorganized architecture, and pathological mitoses — hallmarks of uncontrolled proliferation and genomic instability.

Prompt Grounding rubric:
level_1: No experimental table, no microscope, no tissue section visible; or scene is unrelated (e.g., human body, CT scan, petri dish with colonies); core objects entirely missing.
level_2: At least one of: experimental table present, microscope present, or tissue section present, but not all three; any two objects appear in incompatible spatial relationship (e.g., microscope floating mid-air, tissue section placed on floor), or core action (placement + observation) is absent or unidentifiable.
level_3: Experimental table, optical microscope, and malignant tumor tissue section are all present and co-located in same scene; tissue section is placed on table (not held, not inside microscope); microscope is positioned adjacent to or above the section; observer viewpoint implies intended observation, but no visible ocular contact or alignment cue (e.g., no eyepiece in frame, no head/eye orientation toward microscope).
level_4: All three core objects present and correctly spatially arranged: tissue section placed flat on experimental table; optical microscope placed stably beside or directly over section; microscope eyepiece is visible in frame and oriented toward viewer's implied line of sight; no object duplication, substitution (e.g., no digital screen instead of optical scope), or mislabeling (e.g., no "normal tissue" label).
level_5: All elements from 4-score are present AND microscope is shown in fixed-viewpoint configuration consistent with standard upright optical microscopy setup (i.e., objective lenses facing downward toward section, stage visible beneath objectives, no inverted/misassembled components); tissue section occupies central region of table within microscope field-of-view zone; no extraneous objects (e.g., gloves, pens, labels) interfering with core spatial triad (table–section–microscope).

Scientific and Causal Correctness rubric:
level_1: Tissue section shows no cellular morphology; appears as uniform solid color (e.g., solid blue, blank white rectangle), or displays non-biological patterns (e.g., grid lines, cartoon cells, text); microscope view is black, blurred, or empty: no observable microstructure.
level_2: Microscope view contains *some* cellular-like shapes (e.g., circles, blobs) but zero morphological hallmarks of malignancy (no nuclear pleomorphism, no irregular nuclei, no high nuclear-cytoplasmic ratio, no mitotic figures); section color is physiologically implausible (e.g., bright green tissue, fluorescent-only without excitation context); or section and view are desynchronized (e.g., section is pink but view is purple).
level_3: Microscope view displays a histological field containing multiple cells; at least one cell exhibits *one* visually verifiable malignant feature: either markedly enlarged nucleus, irregular nuclear contour, or abnormally high nuclear-to-cytoplasmic ratio, but other required features (e.g., loss of polarity, abnormal mitoses) are absent or ambiguous; tissue section staining matches standard H&E (pink cytoplasm, purple-blue nuclei); no staining inversion or monochrome distortion.
level_4: Microscope view contains ≥3 distinct malignant tumor cells, each showing ≥2 of the following simultaneously visible features: (a) nuclear pleomorphism (visibly differing nuclear sizes within same field), (b) hyperchromasia (darker-stained nuclei relative to background), (c) loss of cellular polarity (disorganized arrangement, no glandular/acinar formation), (d) visible abnormal mitotic figure; AND tissue section staining is consistently H&E-concordant across entire visible section surface (no patchy discoloration, no bleed-through artifacts).
level_5: All elements from 4-score are present AND the microscope view shows a continuous, in-focus cellular field where every identifiable nucleus displays a coarse, grainy dark chromatin texture rather than a smooth one, and at least one cell shows an unambiguous abnormal mitosis (a tripolar or multipolar spindle with asymmetrical chromosome separation); no normal epithelial architecture (e.g., an intact gland lumen or continuous basal layer) appears anywhere in the field; the tissue section's surface shows a crisp, well-defined edge with no folding or artifact obscuring the morphology.

Spatiotemporal Consistency rubric:
level_1: The tissue section or the microscope vanishes and reappears between frames, or the tissue section's position on the table jumps to a different spot with no motion connecting the two positions; the microscope's stand or arm visibly snaps, or the table's leg fractures, mid-video; the tissue section or microscope appears to duplicate, with two copies visible in the same frame at once, or one object passes straight through the solid body of the other as if neither had substance; or the shot's lighting abruptly flips (e.g., shadows reverse direction, or the scene jumps from bright to dark) with no light source movement to explain it.
level_2: At least one major spatiotemporal violation is present: the microscope's orientation changes discontinuously (e.g., it appears rotated by roughly 90 degrees in a single cut with no visible rotation shown in between); the tissue section visibly deforms as if it were soft or liquid (bending, flowing, or pulsing) instead of staying a flat, rigid slide; a hand or object occludes the microscope and then reappears in front of it without ever visibly passing behind it; blur obscures the table, microscope, and tissue section all at once for three or more consecutive frames.
level_3: The table stays in the same place, the microscope stays attached to the same spot on or beside the table, and the tissue section stays flat and unmoving on the table for the whole clip; aside from the single prescribed cut or transition from the wide establishing shot to the microscope eyepiece POV, there are no other jump cuts or sudden swings in camera angle; the room's overall brightness doesn't change dramatically from one moment to the next, but a small amount of frame-to-frame wobble in the microscope's or tissue section's outline, or one brief moment where the view softens slightly out of focus and then sharpens again, is acceptable as long as nothing changes position, appears, or disappears.
level_4: All elements from 3-score are present AND the table, tissue section, and microscope base show no sliding relative to one another even if the camera moves (they shift together, keeping the same spacing between them); the room's brightness does not flicker at any point in the clip; the edges of the microscope body and the tissue section stay crisp and well defined rather than breaking up or shimmering; there is no noticeable delay between an adjustment shown at the microscope (e.g., a focus knob turning) and the corresponding change in the eyepiece view; other than the single prescribed transition into the eyepiece POV, the shot that follows holds a fixed framing with no additional pans, tilts, zooms, or cuts.
level_5: All elements from 4-score are present AND the entire clip shows no motion-smoothing artifacts (no ghost trails behind moving parts, no object appearing twice within the same frame); reflections on the microscope's metal parts stay geometrically consistent with the rest of the scene as the camera or objects move; playback looks like one continuous, evenly paced recording with no stutter or skipped moments; aside from the single prescribed cut/transition from the wide establishing shot into the microscope eyepiece POV, the camera's framing stays exactly fixed for the remainder of the clip, with no panning, tilting, zooming, or dollying at any point.