Domain: Engineering — Biomedical Engineering
Target concept: Thermal Effects on Cells

Video generation prompt: Three petri dishes containing human red blood cell saline suspensions were placed in a water bath at 37°C (normal), 42°C (mild hyperthermia), and 48°C (severe hyperthermia), from left to right, respectively. After incubation for 5 minutes, the morphology of red blood cells was observed under a microscope. Normal red blood cells were marked in pink, while ruptured red blood cells were marked in colorless. The three microscopic fields were displayed side by side, with the left field corresponding to the 37°C water bath, the middle field to the 42°C water bath, and the right field to the 48°C water bath.

Evaluation guide:
(i) Target concept: Temperature-dependent hemolysis of human red blood cells in isotonic saline.
Minimum mechanism: Thermal energy disrupts spectrin-ankyrin cytoskeleton and phospholipid packing → loss of membrane cohesion → osmotic lysis → release of hemoglobin → optical transparency (colorless appearance) in suspension under microscopy.
(ii)Storyline: [Frame 0–1] Static overhead view reveals three identically sized petri dishes on water bath surface, labeled 37°C (left), 42°C (middle), 48°C (right). [Frame 2–3] Cut to fixed-magnification microscope view: three adjacent, non-overlapping circular fields fill screen horizontally; each field displays suspension medium with cellular structures. [Frame 4–9] Visual differentiation emerges: left field contains exclusively closed, smooth, pink-stained biconcave discs; middle field contains interspersed pink discs and amorphous colorless zones; right field contains only colorless zones with no pink structures. No camera motion, no zoom, no pan; all fields remain simultaneously visible and geometrically aligned throughout. Visibility constraint: all morphology and marking must be resolvable without annotation aids—no arrows, no callouts, no text overlays beyond temperature labels.

Manual:
Experimental Procedure
Three petri dishes with human red blood cell saline suspensions placed in water baths at 37°C, 42°C, and 48°C (left to right). After 5-minute incubation, samples observed under microscope.
Observation of Experimental Phenomena
Side-by-side microscopic fields: left field (37°C) shows only pink-marked intact biconcave red blood cells; middle field (42°C) shows mixture of pink-marked intact and colorless-marked ruptured cells; right field (48°C) shows only colorless-marked ruptured cells (no pink cells).
Summary of Experimental Principles
Thermal stress denatures membrane proteins and disrupts lipid bilayer integrity: mild hyperthermia (42°C) causes partial hemolysis; severe hyperthermia (48°C) causes complete hemolysis; normothermia (37°C) maintains membrane stability and cell morphology.

Prompt Grounding rubric:
level_1: None of the three required elements appears: no petri dishes on a water bath, no three-way left-middle-right layout, no 37°C/42°C/48°C labels, and no pink/colorless marking scheme used anywhere in the video.
level_2: Fewer than three petri dishes appear, or three appear but not arranged left-to-right; the three temperature labels are missing or attached to the wrong dishes (e.g., a 48°C label on the leftmost dish); the three microscope fields are not shown side by side in one fixed view; or no pink/colorless marking scheme is used at all.
level_3: Three petri dishes sit on the water bath in left-middle-right order; the labels 37°C, 42°C, and 48°C each sit next to one dish in that same left-to-right order; three microscope fields are shown together in one fixed camera view; every field contains visible round-cell material; a pink/colorless marking scheme is used somewhere across the three fields.
level_4: Everything at the 3-point level holds, and additionally: the three microscope fields share the same magnification, orientation, and framing as each other (no field is rotated, zoomed in further, or angled differently than the others); no pipette, glove, extra label, or other object intrudes into the three-field display; the three fields stay simultaneously visible together for the whole sequence rather than being shown one at a time.
level_5: Everything at the 4-point level holds, and additionally: all three fields share the same background tone, brightness, and focus sharpness as each other; the boundary between adjacent fields stays clean with no overlap, gap, or visual artifact joining them; the temperature labels stay legible and correctly positioned next to their dish for the entire video; no field flickers, blurs, or otherwise looks inconsistent with its neighbors in image quality.

Scientific and Causal Correctness rubric:
level_1: All three fields show the same marking and cell appearance regardless of the labeled temperature (e.g., every field is entirely pink, or every field is entirely colorless), or a field's marking directly contradicts its temperature label (e.g., the 48°C field shows only pink, fully intact-looking cells).
level_2: The three fields differ from each other, but the marking colors are placed on the wrong fields relative to the temperature gradient: e.g., colorless (ruptured) marking shows up in the 37°C field, or pink (intact) marking shows up in the 48°C field, or the 42°C field looks identical to either extreme instead of showing an in-between mix.
level_3: The 37°C (left) field shows only pink-marked, smooth disc-shaped cells; the 48°C (right) field shows only colorless-marked regions with no pink cells; the 42°C (middle) field shows a visible mix of both pink and colorless; but at least one field also contains ambiguous shapes (e.g., fragmented pink patches or shapeless colorless blobs that are hard to read as cells).
level_4: Everything at the 3-point level holds, and additionally: the pink-marked cells in the left and middle fields consistently look like whole, closed, smooth-edged discs rather than fragments; the colorless regions in the middle and right fields consistently look like clear, cell-shaped voids rather than scattered debris; each field is dominated by the marking pattern its temperature would predict, without scattered stray patches of the wrong marking color breaking up that pattern.
level_5: Everything at the 4-point level holds, and additionally: the left field shows no visible patch of colorless marking anywhere within its cells; the right field shows no visible patch of pink marking anywhere in the frame; the middle field's pink and colorless regions have a clean, readable boundary between them rather than a smeared transition; the pink color looks the same shade across all fields that contain it, and the colorless regions look equally clear/transparent across all fields that contain them.

Spatiotemporal Consistency rubric:
level_1: The three fields change position or size relative to each other between moments with no motion shown getting there; one or more fields vanish and reappear; the overall lighting jumps abruptly partway through; a petri dish container snaps to a new position or angle without moving there; a field's border flickers or warps; or the three fields are shown from inconsistent viewpoints (e.g., one field top-down, another at an angle).
level_2: At least one field visibly changes scale or aspect ratio during playback; the field layout wobbles or jitters enough to notice; brightness visibly differs between fields within the same frame; a container's edge flickers in and out; or something (e.g., a hand) enters the frame and vanishes without ever leaving it.
level_3: All three fields stay visible for the entire video in the same left-middle-right order; the petri dishes stay in place without moving or rotating; nothing teleports or suddenly changes scale; the lighting inside each frame stays steady; any drift in the fields' alignment relative to each other is slight enough that a viewer would need to compare frames closely to notice it, and any softening/sharpening of focus is brief and mild rather than a persistent blur.
level_4: Everything at the 3-point level holds, and additionally: the three fields stay visibly aligned with each other with no drift a viewer would notice while watching normally; none of the fields shows extra vignetting, color fringing, or edge distortion that the others lack; the brightness and evenness of lighting look the same across all three fields in every frame; nothing in a still field shows motion blur; and the fields do not appear to shift relative to each other as if the camera itself were moving.
level_5: Everything at the 4-point level holds, and additionally: all three fields share the same apparent depth of field and background blur as each other; no field shows visibly more noise or compression blockiness than the others; nothing in the video repeats or skips; the contrast and overall brightness stay matched across the three fields for the entire sequence; and the outer rim of each dish/field stays a clean, unbroken shape throughout, with no visible shaking or vibration of the field boundaries.