Domain: Healthcare — Pathophysiology
Target concept: Hyaline degeneration of muscle tissue

Video generation prompt: A slide of hyaline degeneration (skeletal muscle) of muscle tissue is placed on the experimental table, stained with hematoxylin and eosin (H&E), and observed under a light microscope.

Evaluation guide:
(i) Target concept: Visual identification of hyaline degeneration in H&E-stained skeletal muscle via light microscopy.
Minimum mechanism: Skeletal muscle tissue must be present and properly sectioned; H&E staining must yield differential nuclear (hematoxylin) and cytoplasmic (eosin) contrast; hyaline change requires protein coagulation producing optically homogeneous, refractile, eosinophilic material that obliterates myofibrillar architecture.
(ii)Storyline: [Frame 0–2] Slide placed flat on table with label facing up; [Frame 3–4] Microscope moved into adjacent, fixed position with objective lens centered over slide; [Frame 5–10] Eyepiece view stabilizes on skeletal muscle tissue showing normal fibers (striated, peripheral nuclei) transitioning into hyaline zones (homogeneous pink, non-striated, acellular); all transitions occur within same continuous field-of-view, no cuts or repositioning; perspective remains fixed at observer-level height, no aerial or oblique angles; visibility constrained to what would be seen through standard 10× or 20× objective (no subcellular ultrastructure, no 3D depth cues beyond focal plane).

Manual:
Experimental Procedure
1. Place H&E-stained skeletal muscle slide on experimental table.
2. Position light microscope adjacent to slide with eyepiece aligned over tissue.
3. Observe under microscope for hyaline degeneration features.
Observation of Experimental Phenomena
Hyaline degeneration appears as homogeneous, eosinophilic (pink), glassy-appearing material within skeletal muscle fibers; striations are lost in affected areas; nuclei are pyknotic or absent within hyaline zones.
Summary of Experimental Principles
Hyaline degeneration is a pathological accumulation of homogeneous, glassy, eosinophilic proteinaceous material in injured skeletal muscle fibers, resulting from coagulative denaturation of contractile proteins; it is identified histologically by loss of striations, absence of nuclei in affected zones, and characteristic H&E staining.

Prompt Grounding rubric:
level_1: No slide present on experimental table; no microscope visible; no H&E-stained tissue shown; no placement or observation action depicted.
level_2: Slide is present on table but not labeled or identifiable as skeletal muscle; microscope is present but not oriented toward slide; H&E staining coloration is absent or unrecognizable (e.g., uniform blue/pink without tissue architecture).
level_3: A glass slide with tissue section is placed on the table; light microscope is positioned adjacent to it; tissue appears stained with hematoxylin (blue-purple nuclei) and eosin (pink cytoplasm); no explicit indication that the tissue is skeletal muscle (e.g., no striations, no multinucleated fibers visible).
level_4: Slide is placed on table; microscope is correctly positioned with eyepiece aligned toward slide; tissue shows clear H&E staining (blue-purple nuclei + pink cytoplasm); skeletal muscle morphology is partially visible (e.g., elongated cells present, but striations or peripheral nuclei not clearly resolved).
level_5: Slide is placed centrally on experimental table; microscope is fixed in side-by-side spatial relationship with slide, eyepiece oriented directly over tissue; tissue exhibits definitive skeletal muscle features: elongated, unbranched, striated fibers with peripheral, oval nuclei; hematoxylin stains nuclei blue-purple, eosin stains sarcoplasm pink; no other tissue types or artifacts present.

Scientific and Causal Correctness rubric:
level_1: No hyaline degeneration present; tissue appears histologically normal or shows unrelated pathology (e.g., necrosis, inflammation, fat infiltration); no color or structural changes consistent with hyaline change.
level_2: Tissue shows generalized pallor or homogeneity but lacks hallmark features of hyaline degeneration; no eosinophilic, glassy, homogeneous material replacing muscle fibers; no loss of striations or nuclear pyknosis.
level_3: Focal areas of homogeneous, eosinophilic (pink), glassy-appearing material are present within muscle fibers; striations are partially obscured in those areas; nuclei may appear shrunken or displaced but are not completely absent.
level_4: Hyaline degeneration is present in multiple muscle fibers: eosinophilic, acellular, glassy material replaces sarcoplasm; striations are fully lost in affected fibers; nuclei are pyknotic or absent within hyaline zones; surrounding non-degenerated muscle fibers retain normal striations and basophilic nuclei.
level_5: All hyaline degenerated fibers show identical morphological hallmarks: complete loss of cross-striations, uniform eosinophilic translucency (no granularity or vacuolation), sharp demarcation from adjacent viable muscle, absence of inflammatory infiltrate within hyaline zones, and preservation of basal lamina outline; no overinterpretation (e.g., no collagen deposition misidentified as hyaline).

Spatiotemporal Consistency rubric:
level_1: The glass slide holding the skeletal-muscle section suddenly disappears from the table or appears out of nowhere; the microscope's position on the table changes to a different spot with no visible movement shown; the brightness or color of the light abruptly flickers or shifts partway through the clip with no camera cut to explain it; the skeletal-muscle tissue visible through the eyepiece switches to a different region with no pan or zoom shown.
level_2: The slide and microscope are both visible, but their fixed side-by-side arrangement breaks down: the slide slides sideways relative to the microscope between two frames; the tissue seen through the eyepiece drifts off-center from where the objective lens is aimed; objects behind the slide and microscope, such as the tabletop edge or background equipment, shift position or bend out of shape with no explanation.
level_3: The camera viewpoint never moves for the whole clip; the slide stays put on the table; the microscope stays in the same adjacent spot; the tissue region visible through the eyepiece (showing muscle fibers transitioning into the hyaline zone) stays the same across the clip; a small amount of continuous wobble, or the image briefly going soft-focus and refocusing, is acceptable.
level_4: The camera viewpoint stays completely fixed; the slide and microscope stay motionless and in the same rigid arrangement relative to each other; the eyepiece view keeps showing the same muscle-fiber and hyaline-zone region with no sudden crop, zoom, or rotation; the brightness on the slide and the shadow cast by the microscope's arm stay the same strength and direction from the first frame to the last.
level_5: No warping is visible anywhere in the shot, and the slide or tissue image never shakes or trembles; the view never shifts sideways as if the camera had moved, no lens flare appears or disappears partway through, and the sharpness of the tissue stays even rather than drifting in and out of focus; the slide surface, the microscope's stage, and the eyepiece keep the same rigid physical relationship to one another in every frame; the muscle-fiber texture and the border of the hyaline zone render smoothly throughout with no flickering or jagged edges appearing and disappearing.