Domain: Healthcare — Nutrition and Food Hygiene
Target concept: Detection of Artificial Pigment (Tartrazine)

Video generation prompt: A test tube containing 10 mL of candy extract solution with artificial pigment (tartrazine) was treated with polyamide powder, shaken for 5 minutes, filtered, and the image was continuously captured with the test tube in focus.

Evaluation guide:
(i) Target concept: Adsorptive removal of artificial food dye (tartrazine) from solution using polyamide as selective solid-phase adsorbent.
Minimum mechanism: Tartrazine molecules must physically adhere to polyamide surface upon contact and agitation; this binding must reduce dissolved dye concentration, resulting in measurable color lightening of supernatant/filtrate and visible dye loading on solid phase.
(ii)Storyline: [Frame 0–2s] Static shot: test tube centered, filled with homogeneous bright yellow liquid. [Frame 2–3s] White polyamide powder dispensed into tube, visible as discrete granules entering liquid. [Frame 3–6s] Continuous shaking: tube oscillates, liquid turbidity increases, powder darkens progressively, liquid hue lightens. [Frame 6–8s] Filtration begins: cloudy suspension poured onto filter; yellow solids retained visibly on paper. [Frame 8–10s] Final still: clear, pale-yellow filtrate collected below; filter paper shows dense yellow deposit. Perspective: Fixed frontal close-up, shallow depth-of-field isolating tube and immediate apparatus; all critical states and transitions fully visible without cutaways or occlusion.

Manual:
Experimental Procedure
1. Place 10 mL candy extract solution (tartrazine) into a clean test tube.
2. Add polyamide powder to the tube.
3. Shake tube vigorously for 5 minutes.
4. Filter the mixture through filter paper.
5. Capture continuous footage with test tube in focus throughout.
Observation of Experimental Phenomena
- Polyamide powder turns yellow upon contact with solution.
- Solution color visibly lightens during shaking.
- After filtration, filtrate is paler and transparent; retained powder on filter paper is intensely colored.
Summary of Experimental Principles
Tartrazine (E102) binds selectively to polyamide via hydrogen bonding and hydrophobic interaction; adsorption removes pigment from solution, enabling detection and quantification in food hygiene testing.

Prompt Grounding rubric:
level_1: No test tube is shown; there's no liquid meant to represent the candy extract; no polyamide powder appears; neither shaking nor filtration is shown; the tube is never kept in view.
level_2: A test tube is present, but something essential is missing or wrong: it holds no liquid or the wrong liquid (e.g., clear water instead of a colored dye solution), the powder added is clearly not a fine white powder (e.g., it looks like sugar or salt), no shaking is shown, no filtration step is implied at all, or the tube is out of focus for most of the video.
level_3: A test tube holding a colored liquid is shown; a white powder representing the polyamide adsorbent is added to it; the tube is visibly shaken; a filter paper or funnel appears next to or under the tube, implying a filtration step; the tube stays in focus for most of the video.
level_4: Everything at the 3-point level is present, and further: the liquid volume looks like roughly 10 mL in a standard test tube, well below the rim; the liquid is a bright yellow color consistent with a tartrazine solution; the added powder is clearly white and granular; the shaking is sustained and clearly visible rather than a brief nudge; the filtration step is explicitly shown, with liquid passing through the filter into a separate vessel; the tube stays sharply in focus the entire time.
level_5: Everything at the 4-point level is present, and further: the powder is added before shaking begins, and shaking happens before filtration, so the steps occur in the correct order; the filtered liquid is collected within the same continuous shot rather than cutting away; no unrelated objects enter the frame; the test tube stays in the same position and orientation throughout.

Scientific and Causal Correctness rubric:
level_1: The liquid's color does not meaningfully change at any point: it stays an even, saturated yellow throughout; the polyamide powder stays white and evenly dispersed with no sign of taking up color; nothing in the video suggests the powder is drawing dye out of the liquid.
level_2: The powder is added and the tube is shaken, but the liquid's color never gets lighter; the powder settles as loose, separate grains without clumping together; the filtrate looks just as saturated and yellow as the original solution; filtration removes no visible haze or particles.
level_3: The polyamide powder clumps into visible aggregates while being shaken; the liquid noticeably grows cloudier and paler in yellow color during that shaking; after filtration the filtrate looks less saturated than the original solution; the powder itself appears to be taking on some of the yellow color, suggesting dye transfer onto the solid.
level_4: Everything at the 3-point level holds, and further: the powder clearly turns yellow as shaking continues; the liquid becomes visibly paler and clearer during agitation, not just cloudier; after filtration the filtrate is distinctly pale and clear; the powder caught on the filter paper is strongly yellow; the sequence reads as powder added, then contact during shaking, then dye drawn onto the powder, then a paler filtrate.
level_5: Everything at the 4-point level holds, and further: the color transfer to the powder is unmistakable, going from plain white to a strong, saturated yellow; the liquid does not start lightening until after the powder has been added and shaking has begun; the final filtrate looks essentially free of visible particles; the yellow color does not return to the filtrate once it has lightened; the powder's granules clump together and settle out over time in a way that looks like ordinary gravity-driven settling under shaking, not some unrelated motion.

Spatiotemporal Consistency rubric:
level_1: The test tube abruptly snaps to a different spot or orientation between frames instead of moving continuously; the room lighting suddenly flips between bright and dark; the liquid's level or shape suddenly changes between frames with no pouring, shaking, or tilting shown to cause it; the powder appears or disappears with no visible motion bringing it in or out; the filter-and-funnel setup changes shape or arrangement between shots.
level_2: The tube wobbles unnaturally or spins far more than the shaking motion would explain; the liquid sloshes in ways that don't match how the tube is moving; the powder disperses and then clumps back together in a way that looks scripted rather than physical; the stream of liquid during filtration starts and stops abruptly instead of flowing continuously; the focus jumps in and out rather than shifting smoothly.
level_3: The test tube stays in the same place in the frame; the shaking looks like a continuous, plausible back-and-forth motion rather than jerky jumps; the liquid's surface moves in a way that matches the tube's motion; the powder settles and mixes at a pace consistent with gravity and the shaking rather than instantly; the filtration stream flows steadily without interruption; the tube stays in focus throughout.
level_4: Everything at the 3-point level holds, and further: the tube, the powder, the filter paper, and the collection vessel all keep the same position relative to each other throughout; the lighting stays even with no flicker, sudden shadow jump, or unexplained flash; the tube's motion speeds up and slows down gradually rather than snapping between speeds; nothing is unnaturally clipped or hidden behind another object.
level_5: Everything at the 4-point level holds, and further: the tube's tilt and orientation stay essentially the same from one frame to the next, without random drifting; the curve of the liquid's surface against the tube walls looks consistent with the tube's actual shape at every point; the powder settles and clumps in a way consistent with gravity and the shaking motion, not some unrelated pattern; the filtration stream flows smoothly without unexplained splashing, dripping, or pulsing; the video plays back at one steady, consistent speed with no visible warping or stretching of objects between frames.