Domain: Natural Science — Biochemistry & Molecular Biology
Target concept: Luciferase Bioluminescence Triggered by ATP Addition

Video generation prompt: In a dimly lit laboratory, two transparent vials containing luciferase and luciferin are placed side by side, with red and blue caps respectively; during the recording, ATP is added only to the vial with the red cap.

Evaluation guide:
(i) Target concept: ATP-dependent enzymatic bioluminescence requiring stoichiometric cofactor activation.
Minimum mechanism: Luciferase enzyme must be pre-mixed with luciferin in both vials; ATP must be exogenously supplied to trigger reaction only where added; light emission arises solely from chemiluminescent oxidation, not fluorescence or external excitation.
(ii)Storyline: Frame 0–3: Static shot of two side-by-side vials with red and blue caps, dim ambient lighting, no emission. Frame 4–6: Pipette enters frame and delivers liquid exclusively into red-capped vial. Frame 7–10: Yellow-green bioluminescence emerges and intensifies only within red-capped vial liquid; blue-capped vial remains absolutely dark; no light spillover, no cap glow, no background illumination change; viewpoint fixed, no occlusion.

Manual:
Experimental Procedure
1. Place two identical transparent vials side-by-side on a lab bench in dim ambient light.
2. Fit one vial with a red cap, the other with a blue cap.
3. Both vials contain luciferase and luciferin in aqueous solution.
4. Add ATP solution via pipette exclusively into the red-capped vial.
Observation of Experimental Phenomena
Only the red-capped vial emits yellow-green bioluminescent light after ATP addition; the blue-capped vial remains completely dark.
Summary of Experimental Principles
Luciferase catalyzes oxidation of luciferin using ATP as an energy cofactor, producing light; absence of ATP prevents reaction — thus only the ATP-supplemented vial luminesces.

Prompt Grounding rubric:
level_1: No vials are shown; or vials appear without red/blue caps; or only one vial is visible; or the caps aren't clearly red and blue; or no addition of ATP into a vial is shown at all.
level_2: Two transparent vials with visible caps appear, but the cap colors are wrong: both red, both blue, or some other color pairing (e.g. green/yellow) instead of one red and one blue; or ATP is added to both vials, or to neither; or the vials are not shown side by side in the same shot.
level_3: Two transparent vials sit side by side in the same shot, one with a clearly red cap and one with a clearly blue cap; a liquid is added into exactly one vial, the red-capped one; no other containers or actions appear in the shot.
level_4: Everything in score 3 continues to hold, and further, the red-capped and blue-capped vials look the same in size and shape except for the cap color, the addition into the red-capped vial is done with a visible dropper or pipette, and nothing (labels, gloved hands, other objects) blocks the view of either cap or vial.
level_5: Everything in score 4 continues to hold, and further, the red cap stays a clear, saturated red rather than drifting toward pink, maroon, or orange, the blue cap stays a clear, saturated blue rather than drifting toward cyan, purple, or teal, both cap colors stay the same across the entire video, and the pipette tip only ever touches the opening of the red-capped vial, never the blue-capped one.

Scientific and Causal Correctness rubric:
level_1: Neither vial glows at any point; or both vials glow at the same time; or a vial glows before anything is added to it; or the light that appears is a color other than yellow-green (e.g. plain white, blue, or red) instead of the yellow-green typical of a luciferase reaction.
level_2: Glowing happens only after something is added, but it happens in the blue-capped vial instead of the red-capped one; or the red-capped vial glows but the blue-capped vial also shows a faint or brief glow; or the light source looks artificial (like an LED, a screen glow, or a reflection) rather than coming from the liquid itself.
level_3: Only the red-capped vial glows, and only after the liquid is added to it; the blue-capped vial never glows at any point; the glow comes from within the liquid itself (no external light source visible) and looks yellow-green rather than white, blue, or red.
level_4: Everything in score 3 continues to hold, and further, the glow builds up gradually rather than flashing on instantly, its brightness keeps increasing for a while after the addition without flickering or dimming partway through, and no glow lingers in either vial after the recording ends if the addition happened near the end.
level_5: Everything in score 4 continues to hold, and further, the glow stays confined to the liquid inside the red-capped vial without spreading onto the cap or the outside of the vial, the blue-capped vial and the space around it stay completely dark for the entire video, and the room around both vials stays evenly dim with no stray light source affecting the contrast between the two vials.

Spatiotemporal Consistency rubric:
level_1: A vial jumps to a different spot between frames; a cap changes color partway through for no reason; a vial flickers between transparent and opaque; the pipette appears or disappears abruptly; the room lighting suddenly changes (e.g. the lights come on).
level_2: One vial visibly rotates or shifts position between frames; the pipette's motion looks jerky rather than smooth; the edge of a cap flickers or strobes oddly from frame to frame; the shadows in the scene point in different directions from one frame to the next even though nothing about the lighting has changed.
level_3: Both vials stay still and keep the same side-by-side arrangement throughout; the pipette enters the frame smoothly and holds a steady angle while delivering liquid; the cap colors and the vials' transparency stay the same; the dim ambient lighting looks the same throughout; nothing in the scene splits apart, warps, or passes through another object.
level_4: Everything in score 3 continues to hold, and further, the pipette's motion looks like one continuous, natural hand movement rather than speeding up or pausing oddly mid-delivery, the highlights on the vial surfaces stay consistent with a light source that isn't moving, and no frame repeats or stutters.
level_5: Everything in score 4 continues to hold, and further, the motion throughout the video looks smooth with no visible shaking or trembling of the vials or pipette, the liquid surface (meniscus) in each vial stays undisturbed until the moment liquid is actually added and only then ripples naturally, and each cap keeps its cylindrical, flat-topped shape without warping across the whole video.