Domain: Healthcare — Pathophysiology
Target concept: Fibroid necrotic connective tissue

Video generation prompt: A section of fibroid necrotic connective tissue is placed on the experimental table, stained with hematoxylin and eosin (H&E), and observed under a light microscope.

Evaluation guide:
(i) Target concept: Microscopic identification of necrotic connective tissue in uterine fibroid via H&E histopathology.
Minimum mechanism: Tissue must be fixed, paraffin-embedded, sectioned, and stained with hematoxylin (nuclear dye) and eosin (cytoplasmic/stromal dye); necrosis must manifest as irreversible structural disintegration detectable at ×100–×400 magnification.
(ii)Storyline: Frame 1–2: Empty experimental table → Frame 3–4: Glass slide with mounted tissue section placed centrally → Frame 5–6: Microscope lowered into position with objective aligned over tissue → Frame 7–8: Eyepiece view activates, revealing focused field → Frame 9–10: Stable microscopic image shows necrotic zone (eosinophilic, acellular) adjacent to viable fibroid (blue nuclei, spindle cells); all transitions must preserve fixed viewpoint, consistent lighting, and anatomical plausibility: no zoom, pan, or rotation; microscope must remain stationary with only focus/adjustment motion affecting eyepiece content.

Manual:
Experimental Procedure
1. Place H&E-stained fibroid tissue section on clean experimental table.
2. Position light microscope directly above slide with objective lens centered over tissue.
3. Look through eyepiece to observe microscopic morphology.
Observation of Experimental Phenomena
Necrotic connective tissue appears as fragmented, intensely eosinophilic collagen bundles with loss of cellular detail; nuclei are pyknotic or absent; ghost cell outlines may be present; adjacent viable fibroid shows spindle-shaped cells with blue-stained nuclei.
Summary of Experimental Principles
Fibroid necrosis involves coagulative or hyaline degeneration of smooth muscle and connective tissue, resulting in eosinophilic, acellular stroma on H&E stain due to protein denaturation and loss of basophilic RNA.

Prompt Grounding rubric:
level_1: No experimental table, no glass slide, no fibroid tissue specimen, and no microscope appear anywhere in the frame; the scene bears no resemblance to a histology bench setup.
level_2: Only one of the required objects appears in isolation (e.g., a bare table with nothing on it, or a microscope with no slide beneath it), and the fibroid tissue section itself is absent or cannot be identified as tissue at all.
level_3: An experimental table holds a rectangular glass slide bearing a pink-and-blue-tinted tissue section, and a microscope is present in the same shot, but the slide is not clearly resting on the table's surface, the microscope's objective is not lined up over the slide, and no eyepiece or magnified view is shown at all.
level_4: The table, the slide with its H&E-colored tissue section, and the microscope are all present and correctly co-located (the slide sits centered on the table and the microscope's objective is aligned vertically above it), but the magnified/eyepiece view is either not shown at all or appears as an empty, out-of-focus circle.
level_5: All setup elements are rendered together correctly: the slide rests centered on the table, the microscope objective is aligned directly over the tissue, and a magnified inset or split-screen view shows a clearly in-focus field with visibly fragmented eosinophilic (pink) collagen, faint ghost-cell outlines, and loss of nuclear detail in the necrotic zone, bordered by intact spindle cells with blue-stained nuclei, all with correct H&E color fidelity and no stray objects cluttering the bench.

Scientific and Causal Correctness rubric:
level_1: The tissue looks healthy, non-fibroid, or unrelated (e.g., glandular, vascular, or fatty tissue); the staining does not resemble H&E at all (uniform purple, green, brown, or grayscale); no connective-tissue architecture is visible.
level_2: The tissue is labeled as fibroid and shows some H&E-like coloring (blue nuclei present), but no necrotic change is shown: the stroma is uniformly pink, cells throughout look intact, and there is no loss of nuclear staining anywhere in the section.
level_3: General H&E coloring is correct (blue nuclei, pink stroma) and the tissue shape is consistent with a fibroid nodule, but the defining necrotic features are missing: the collagen looks intact rather than smudged or fragmented, no acellular (nucleus-free) zone is visible, and no ghost-cell outlines appear anywhere in the section.
level_4: The necrotic zone shows fragmented, intensely eosinophilic (deep pink) collagen bundles with no intact fibroblast nuclei inside it, faint ghost-cell outlines are visible within that zone, and it sits adjacent to a clearly viable region of spindle-shaped smooth-muscle cells with normal blue nuclei; the transition from necrotic to viable tissue reads as one coherent specimen.
level_5: In addition, the necrotic zone's position within the fibroid nodule (central or eccentric, surrounded by compressed but viable smooth muscle) matches how fibroid infarction actually presents, and nothing in the image could be mistaken for necrosis by an artifact instead: no knife-mark tearing, no trapped air bubbles, and no tissue folding rendered in a way that could be misread as a ghost-cell zone.

Spatiotemporal Consistency rubric:
level_1: The slide, microscope, or tissue section is missing from a shot and then fully present again with no placing or removing motion shown, the microscope's body loses or regrows a part (e.g., its stage or arm), or the lighting flickers hard enough to break the sense of one continuous take.
level_2: One clear break in continuity occurs: the slide jumps from the table's edge to its center with no hand or motion carrying it there, the focus knob visibly turns while the eyepiece view stays frozen and unchanged, or the tissue section's outline changes shape between shots with no deformation shown.
level_3: The table, slide, and microscope keep the same relative positions throughout, lighting stays even, and any motion shown is continuous, but one small inconsistency remains, such as the microscope stage nudging slightly with no matching change in the eyepiece view, or a hand placing the slide but pulling away just before it visibly settles.
level_4: The slide-on-table, microscope-over-slide, and eyepiece-aligned-with-tissue relationships hold steady for the whole clip with no popping or jump, and any focus adjustment looks physically continuous, but one small timing mismatch remains, such as the eyepiece view sharpening a beat after the focus knob turns rather than at the same instant.
level_5: The slide settles smoothly onto the table exactly as the placing motion ends, each turn of the focus knob is matched instantly by a corresponding change in sharpness in the eyepiece view, lighting direction and warmth stay constant throughout, and no frame shows a jump, warp, or mismatch between the bench-level action and the microscopic view.