Domain: Natural Science — Morphology
Target concept: Growth under nutrient-complete conditions vs. growth in nitrogen-deficient soil

Video generation prompt: Same seedlings were separately planted in two pots: the blue pot was filled with complete nutrient soil, and the red pot was filled with nitrogen-deficient soil; they were placed side by side within a few weeks, under identical light and watering conditions.

Evaluation guide:
(i) Target concept: Nitrogen limitation induces chlorosis and growth retardation in seedlings due to impaired chlorophyll biosynthesis and reduced protein accumulation.
Minimum mechanism: Seedlings must be genetically identical, developmentally synchronized, and exposed to identical non-nitrogen environmental variables (light intensity/duration, water volume/frequency, temperature, CO₂); soil matrices must differ *only* in nitrogen bioavailability (not pH, texture, or micronutrients).
(ii)Storyline: Frame 0–1: Static wide shot showing two adjacent pots (blue left, red right), each containing one morphologically identical seedling (2–3 cm tall, 1–2 cotyledons, no true leaves). Frame 2–8: Continuous static view (no action, no camera motion) allowing passive observation of differential development: blue pot seedling maintains upright posture, deep green color, and subtle new leaf emergence; red pot seedling exhibits shorter stature, pale yellow-green leaves (especially lower leaves), and reduced rigidity. Frame 9–10: Final frame preserves full visibility of both seedlings and pots; no fade, cut, or annotation. Perspective: Eye-level frontal view, fixed focal plane encompassing both pots entirely; no oblique angles or depth-of-field blur that hides critical features.

Manual:
Experimental Procedure
1. Place identical seedlings into two separate pots.
2. Fill blue pot with nutrient-complete soil; fill red pot with nitrogen-deficient soil.
3. Position pots side-by-side under identical light and watering conditions.
Observation of Experimental Phenomena
After several weeks: seedling in blue pot is taller and greener; seedling in red pot is shorter and shows yellowing (chlorosis) on leaves.
Summary of Experimental Principles
Nitrogen is essential for chlorophyll and protein synthesis; its deficiency impairs photosynthesis and growth, causing stunting and chlorosis.

Prompt Grounding rubric:
level_1: No two pots visible; or only one pot shown; or pots not distinguishable by color; or seedlings absent; or no side-by-side spatial arrangement; or lighting/watering conditions not implied as identical (e.g., visibly different watering, shadow asymmetry, or single light source casting divergent shadows)
level_2: Two pots present and side-by-side, but at least one of the following is missing or incorrect: blue pot is not blue, red pot is not red, seedlings are absent in one or both pots, or seedlings are not same species/size at start (e.g., visibly different morphology, number >1 per pot, or pre-existing leaves inconsistent with seedling stage)
level_3: Two pots visible side-by-side; blue pot is blue-colored; red pot is red-colored; exactly one healthy seedling visible in each pot; seedlings appear morphologically identical at video onset (same height, stem thickness, leaf count ≤2, no visible discoloration); no explicit action (e.g., watering, moving) occurs: static observation setup only
level_4: All 3-point criteria satisfied AND both seedlings remain fully visible throughout entire video without occlusion, cropping, or zoom change; pots maintain fixed relative positions (no sliding, tilting, or repositioning); background is uniform and neutral (no competing objects or text); lighting is consistent across both pots (no unilateral shadow, glare, or brightness difference)
level_5: All 4-point criteria satisfied AND the blue pot and red pot are identically shaped and sized; seedlings are centered in their respective pots; no motion blur, flicker, or inter-frame jitter; frame composition maintains strict symmetry (vertical midline bisects gap between pots; seedlings aligned horizontally at same visual height)

Scientific and Causal Correctness rubric:
level_1: No visible growth difference between seedlings; or both seedlings show identical severe stunting/yellowing; or red pot seedling is larger/greener than blue pot seedling; or discoloration appears on blue pot seedling only
level_2: Growth difference is present but violates causal direction: e.g., red pot seedling shows taller stem or more leaves than blue pot seedling; or yellowing appears only on blue pot seedling; or both seedlings are fully green and identical in size/leaf count
level_3: Red pot seedling is shorter than blue pot seedling AND red pot seedling exhibits yellowing (chlorosis) on leaves/stem while blue pot seedling remains green; no other color anomalies (e.g., purple, brown, black) appear on either seedling; leaf count on red pot seedling ≤ blue pot seedling
level_4: All 3-point criteria satisfied AND yellowing is confined to leaves/stem of red pot seedling (no yellowing on soil, pot, or background); blue pot seedling shows no yellowing whatsoever; stem of red pot seedling is visibly thinner than blue pot seedling; no wilting, necrosis, or leaf curling on either seedling
level_5: All 4-point criteria satisfied AND yellowing on red pot seedling appears specifically on older (lower) leaves first (i.e., apical leaves remain green while basal leaves yellow); blue pot seedling displays ≥1 new leaf unfolding (visible emerging leaf tip or slight curl), confirming active growth; no texture degradation (e.g., papery, translucent, or brittle appearance) on any leaf

Spatiotemporal Consistency rubric:
level_1: Pots or seedlings teleport between frames; or sudden appearance/disappearance of pot/seedling; or abrupt change in pot orientation (e.g., front-facing → tilted 45° without motion); or lighting shifts (e.g., shadow vanishes or jumps) between consecutive frames
level_2: Minor discontinuity: e.g., seedling flickers (appears absent in ≥1 frame then reappears); pot edge warps or pixelates inconsistently; background elements shift laterally without parallax; or shadow shape changes without corresponding light source movement
level_3: All objects maintain continuous presence and stable geometry across all frames; no object occlusion or clipping; pots remain rigidly fixed in position and orientation; lighting and shadow remain spatially coherent (shadows cast consistently downward and toward same direction); no morphological warping (e.g., seedling bending unnaturally without motion)
level_4: All 3-point criteria satisfied AND seedling motion (if any) is smooth and physically plausible (e.g., gentle sway only, no jerking or oscillation beyond natural wind response); pot surfaces reflect consistent material properties (matte soil, non-glossy pot finish); no inter-frame misalignment (e.g., sub-pixel jitter or frame wobble)
level_5: All 4-point criteria satisfied AND zero inter-frame velocity discontinuity (no acceleration spike in sway); no micro-motion artifacts (e.g., shimmer, aliasing, or moiré on leaf veins or soil surface); shadow density and softness remain constant; no depth cue conflict (e.g., foreground pot edge never visually overlaps background pot in a way violating side-by-side layout)