Domain: Healthcare — Pathology
Target concept: Cellular injury (ischemic necrosis)

Video generation prompt: A myocardial tissue specimen subjected to prolonged ischemia was placed on the experimental table for histomorphological observation under a light microscope.

Evaluation guide:
(i) Target concept: Coagulative necrosis in myocardium due to irreversible ischemia.
Minimum mechanism: Prolonged oxygen deprivation → ATP depletion → failure of ion pumps → calcium influx → protease & nuclease activation → cytoskeletal collapse + nuclear degradation → preserved tissue architecture with eosinophilic cytoplasm.
(ii)Storyline: Frame 1–2: Myocardial tissue section placed statically on labeled pathology table. Frame 3–5: Light microscope positioned above specimen with eyepiece aligned. Frame 6–10: Stable close-up view through microscope showing central hypereosinophilic zone (no nuclei, no striations) sharply adjacent to viable myocardium (intact striations, visible nuclei); viewpoint fixed, no panning/zooming; lighting uniform; no external interference (e.g., hands, labels, stains).

Manual:
Experimental Procedure
1. Place formalin-fixed, paraffin-embedded myocardial tissue section on pathology lab table.
2. Position light microscope with 10x objective directly above specimen.
3. Adjust focus to observe histomorphology.
Observation of Experimental Phenomena
Hypereosinophilic myocardial fibers with loss of cross-striations and absent nuclei in central zone; adjacent viable myocardium retains striations and normochromatic nuclei.
Summary of Experimental Principles
Prolonged ischemia causes coagulative necrosis: protein denaturation leads to hypereosinophilia; myocyte lysis results in nuclear pyknosis → karyorrhexis → karyolysis; striations disappear due to sarcomere disintegration.

Prompt Grounding rubric:
level_1: No myocardial tissue specimen visible; no experimental table present; no light microscope in frame; no observation setup implied.
level_2: A biological tissue specimen is present on a surface, but it is not identifiable as myocardial tissue (e.g., lacks cardiac muscle striations or anatomical context); microscope is absent or misidentified (e.g., labeled as electron microscope or absent entirely); table is generic and unassociated with histopathology lab setting.
level_3: A myocardial tissue specimen is placed on an experimental table; a light microscope is present in the scene; the specimen is positioned such that microscopic observation is plausibly intended; no other conflicting objects (e.g., surgical tools, blood, fresh heart) dominate the frame.
level_4: Myocardial tissue specimen is placed centrally on a standard pathology lab table; light microscope is visibly present with eyepiece oriented toward specimen; specimen is static and fully within field of view; no extraneous objects (e.g., gloves, stains, slides not yet mounted) appear during observation phase.
level_5: Myocardial tissue specimen is placed on a labeled pathology lab table; light microscope is correctly configured (binocular, upright, objective lens aligned toward specimen plane); specimen is unmoving, fully in focus plane, and occupies dominant visual area; no occluding elements, no irrelevant equipment, no temporal inconsistency in placement or framing.

Scientific and Causal Correctness rubric:
level_1: Tissue appears healthy (no necrosis features); color is uniform pink/red; no coagulative necrosis morphology; no loss of striations or nuclear pyknosis visible.
level_2: Tissue shows non-ischemic artifacts (e.g., autolysis, freezing artifact, crush artifact); color is inconsistent with formalin-fixed paraffin-embedded myocardium (e.g., blue, purple, or bright red); no recognizable ischemic necrosis features (e.g., hypereosinophilia, ghost cells, nuclear karyolysis).
level_3: Tissue displays characteristic ischemic necrosis: homogeneous hypereosinophilia (pink), loss of cross-striations, and absence of nuclei in affected zones; background tissue retains normal myocardial architecture; no contradictory features (e.g., neutrophilic infiltrate indicating acute inflammation instead of pure ischemia).
level_4: Ischemic necrosis features are spatially coherent: hypereosinophilic zones show sharp demarcation from viable myocardium; ghost cells appear without residual nuclei; no mixed patterns (e.g., no hemorrhage, no granulation tissue, no reperfusion injury signs like contraction bands); all necrotic features match prolonged ischemia (not early or late infarction stages).
level_5: All ischemic necrosis features are fully consistent with prolonged ischemia: complete loss of striations and nuclei in central zone; uniform hypereosinophilia without mottling; adjacent viable myocardium shows intact striations and normochromatic nuclei; zero contradictory histomorphological features (e.g., no calcification, no fibrosis, no inflammatory cells, no vascular thrombi visible).

Spatiotemporal Consistency rubric:
level_1: Specimen teleports between frames; microscope disappears/reappears; lighting changes abruptly (e.g., flash, flicker, total shadow shift); table surface warps or fractures across frames.
level_2: Specimen shifts position without cause (e.g., slides sideways without contact); microscope angle changes discontinuously; lighting gradient flips direction mid-sequence; specimen edges exhibit temporal aliasing (e.g., strobing, tearing).
level_3: Specimen remains stationary on table throughout; microscope stays fixed in position and orientation; lighting is stable and uniform across all frames; no structural distortion of tissue or table; no inter-frame jitter or pop-in/pop-out of objects.
level_4: Specimen remains stationary; microscope remains fixed; lighting is stable and matches typical pathology lab illumination (cool white, diffuse, no glare on tissue surface); tissue surface shows consistent texture and reflectivity across frames; no motion blur inconsistent with stillness.
level_5: Zero spatiotemporal anomalies: specimen position, scale, and perspective remain identical across all frames; microscope and table share rigid spatial relationship (no parallax drift); lighting cast shadows consistently; tissue surface microtexture (e.g., eosin granularity) is temporally stable and continuous; no compression artifacts, no frame duplication, no interpolation ghosts.