Domain: Healthcare — Pathology
Target concept: Dysplasia (hyaline degeneration)

Video generation prompt: A damaged heart valve tissue specimen is placed on the experimental table, and changes in the collagen fiber regions are observed under the microscope.

Evaluation guide:
(i) Target concept: Visual demonstration of hyaline degeneration in collagen fibers of a damaged heart valve specimen, as defined by histopathological criteria (acellular, homogeneous, eosinophilic, glassy appearance). Minimum mechanism: The tissue must be stained with hematoxylin and eosin (H&E); the microscope must resolve subcellular detail; hyaline zones must arise from collagen fibers (not amyloid or mucin) and exhibit optical properties consistent with proteinaceous homogeneity and loss of fibrillar architecture.
(ii)Storyline: [Phase 1: Macro Setup] Damaged heart valve tissue specimen rests centrally on experimental table, well-lit, with standard microscope positioned adjacent and upright. [Phase 2: Micro Engagement] Microscope stage holds H&E-stained tissue section; focus adjustment leads to sharp field of view. [Phase 3: Phenomenon Reveal] Field shows juxtaposed regions: left side displays intact collagen (wavy, blue-purple, fibrillar); right side displays hyaline degeneration (smooth, pale pink, structureless, glassy). [Phase 4: Causal Anchoring] Both regions originate from same valve specimen; transition zone is continuous and anatomically contiguous; no intervening labels or annotations required; visual contrast alone must support pathological interpretation. Perspective is fixed (no camera orbit); visibility requires full clarity of both macro context and micro detail within 10-second duration.

Manual:
Experimental Procedure
1. Place damaged heart valve tissue specimen on clean experimental table.
2. Position standard light microscope over specimen; adjust focus to visualize tissue section at medium magnification.
3. Observe collagen fiber regions under microscope.
Observation of Experimental Phenomena
Collagen fiber regions appear homogeneous, acellular, pale pink (eosinophilic), and glassy (hyaline), sharply contrasting with adjacent non-degenerated collagen fibers that retain wavy, basophilic (blue-purple), fibrillar structure.
Summary of Experimental Principles
Hyaline degeneration is a form of connective tissue injury characterized by accumulation of homogeneous, glassy, eosinophilic material in collagen fibers — commonly seen in chronically damaged heart valves. It reflects irreversible collagen denaturation and protein deposition.

Prompt Grounding rubric:
level_1: No heart valve tissue specimen is visible; no experimental table appears; no microscope is shown; there is no depiction of the specimen being placed anywhere; and no field of view resembling a tissue section is identifiable at any point.
level_2: The heart valve specimen appears but is never placed on an experimental table; or the microscope is present but looks non-functional (e.g., lens cap on, no eyepiece visible, empty stage); no observation activity is depicted; and no framed tissue field is identifiable.
level_3: The damaged heart valve specimen is shown placed on the experimental table; the microscope is present with correct upright orientation and a visible stage; the field of view shows some tissue section, but it is not clear enough to make out any collagen fiber detail (e.g., out of focus, wrong magnification, or too dark).
level_4: Everything in score 3 holds AND the eyepiece view is in sharp focus and clearly shows a tissue section with visible collagen fiber regions (whatever their appearance); however, either the side-by-side comparison layout is missing, or the shot does not keep both the macro (specimen-on-table) view and the micro (eyepiece) view within one continuous, spatially linked sequence.
level_5: Everything in score 4 holds AND the shot keeps a fixed viewpoint that shows both the specimen on the table and the microscope's eyepiece field (via split-screen, inset, or an unbroken pan/zoom), with no gap in spatial continuity between the two views, no hands or extraneous objects blocking either view, and the eyepiece field staying in focus for the entire time it is shown.

Scientific and Causal Correctness rubric:
level_1: The collagen-fiber region shows no structural change at all: it keeps a uniform, typical pinkish-purple hematoxylin-eosin appearance with nothing resembling hyaline degeneration; the tissue reads as histologically normal or shows an unrelated finding such as inflammation or calcification instead.
level_2: The collagen-fiber region shows uneven staining but its wavy fibrillar shape is still intact; there is no smooth, glassy texture anywhere; the coloring pattern (e.g., patchy blue rather than diffuse pink) does not match hyaline degeneration; and nothing in the frame visually ties the valve damage to any collagen change.
level_3: The collagen-fiber region looks pink (eosinophilic) and largely smoothed out, but faint outlines of the original fibers are still visible; the loss of structure is incomplete, so the glassy, acellular quality expected of hyaline degeneration is only partly there; no adjacent normal collagen is shown for contrast.
level_4: The collagen-fiber region is fully smooth, acellular, and evenly pink, with no trace of the original fiber pattern left, matching hyaline degeneration; the collagen right next to it keeps a clear wavy fiber pattern and a stronger blue-purple stain; the two sit next to each other in a way that visually suggests the damaged valve caused the change, though nothing in the frame explicitly labels or confirms this.
level_5: The collagen-fiber region shows textbook hyaline degeneration: fully homogeneous, acellular, pale pink, glassy zones with sharp borders; the adjacent non-degenerated collagen keeps intact, wavy, basophilic (blue-purple) fibrils; the transition between the two zones is continuous and anatomically plausible; no artifact (folding, bubbles, overstaining) distorts the hyaline appearance; and every one of these features (homogeneity, acellularity, eosinophilia, glassy refractility, and sharp contrast with the adjacent fibrillar collagen) holds without exception anywhere in the degenerated zone.

Spatiotemporal Consistency rubric:
level_1: The valve specimen or the microscope jumps from one spot to another with no motion in between; the eyepiece view appears or disappears in a single frame with no transition; the tissue visibly melts or stretches out of its true shape; the overall lighting swings abruptly between bright and dark within a single frame with no adjustment or cause shown; or the view snaps from the macro table shot straight to the micro eyepiece shot with no zoom, pan, dissolve, or simultaneous split-screen/inset connecting them.
level_2: The specimen slides across the table with no hand or tool moving it; the microscope stage shifts position while the focus stays untouched; the shadow cast beneath the specimen swings to the opposite side with no light source moving; the tissue texture flickers between grainy and smooth; or the cut from the table view to the eyepiece view happens abruptly with no zoom, dissolve, or fixed split-screen/inset layout connecting them.
level_3: The specimen stays put on the table and the microscope stays still; the brightness on the specimen and stage stays at one level and the shadow it casts keeps the same direction from the first frame to the last; both the macro (specimen-on-table) view and the micro (eyepiece) view are shown with visible spatial continuity (either via a split-screen/inset held in a stable layout, or via a visible zoom or pan connecting the two), but there is a brief motion blur during a zoom, a hand passes through and out of frame, or the background briefly falls out of focus while the specimen does not (or vice versa).
level_4: Everything in score 3 holds AND, if a zoom/pan is used, it has no blur or hand-occlusion at all; if a split-screen/inset is used instead, its layout stays fixed and aligned to the same spot on the specimen throughout; the specimen is sharply focused against a softly blurred background in the table shot and the tissue section is sharply focused against an out-of-focus stage in the eyepiece shot, with at most one small, one-time flaw such as a brief glint on the lens or a slight wobble in the zoom path (if a zoom is used).
level_5: Everything in score 4 holds with no exception: the specimen's position, size, and angle stay geometrically identical across every frame; the microscope never moves on its own; the brightness and shadow direction on both the table and the stage never change; if a zoom/pan is used, the focus pull between the table and eyepiece views is smooth and physically continuous; if the eyepiece view is a split-screen or inset instead, both views stay simultaneously sharp and aligned to the same spot on the specimen throughout.