Domain: Healthcare — Microbiology-Immunology
Target concept: Lactophenol Cotton Blue Staining (for Fungal Observation)

Video generation prompt: Place a drop of lactophenol cotton blue stain on a clean slide. Use an inoculating needle to collect a small amount of the fungal specimen, gently disperse the specimen, and carefully cover it with a coverslip. Observe under a microscope, starting from low magnification and gradually increasing to high magnification.

Evaluation guide:
(i) Target concept: Visual verification of correct wet-mount preparation and microscopic visualization of fungi using lactophenol cotton blue stain.
Minimum mechanism: Aqueous stain must remain liquid and spreadable on glass; fungal specimen must retain structural integrity (not lysed or dissolved); coverslip must seal specimen without compression or displacement; microscope optics must resolve stained chitinous structures without optical artifact.
(ii)Storyline: [Frame 0–2] Clean slide enters view → droplet of blue liquid stain placed centrally. [Frame 3–4] Inoculating needle enters frame, contacts fungal culture, withdraws with visible specimen load. [Frame 5–6] Needle tip touches stain droplet and gently agitates; specimen disperses visibly. [Frame 7] Coverslip descends vertically, aligns flush, and settles without shifting or trapping bubbles. [Frame 8–10] Microscope eyepiece view appears: first low-mag field showing diffuse blue area with filamentous shapes, then smooth zoom-in to high-mag field revealing branched, septate, uniformly blue hyphae; no interruption in specimen visibility or staining fidelity throughout.

Manual:
Experimental Procedure
1. Place a single drop of lactophenol cotton blue stain on a clean glass slide.
2. Use an inoculating needle to collect a small amount of fungal specimen.
3. Gently disperse the specimen into the stain droplet.
4. Carefully lower a coverslip onto the stained specimen.
5. Observe under microscope: start at low magnification, then increase to high magnification.
Observation of Experimental Phenomena
Blue-stained fungal hyphae and/or spores appear sharply contrasted against pale background; hyphae show branching and septa; spores are round/oval and uniformly blue; no air bubbles or stain overflow under coverslip.
Summary of Experimental Principles
Lactophenol cotton blue preserves fungal morphology and stains chitin in cell walls blue, enabling clear microscopic identification.

Prompt Grounding rubric:
level_1: Fewer than two of the five required elements (clean slide, lactophenol cotton blue stain droplet, inoculating needle, fungal specimen, coverslip) appear anywhere in the clip.
level_2: At least two of the five required elements are visible, but none of the staining actions occur in sequence: no stain droplet is placed, no specimen is collected by the needle, no dispersion happens, no coverslip is applied, and no microscope observation begins.
level_3: All five required elements are present, and the sequence begins correctly (a stain droplet is placed on the slide and the needle contacts and lifts the fungal specimen), but visible dispersion of the specimen into the droplet is missing or unclear, or the coverslip is never placed or is placed badly misaligned, or no microscope view is shown.
level_4: All five elements are present and all five actions occur in the correct order: stain droplet placed on the clean slide, specimen collected by the needle, specimen visibly dispersed into the droplet, coverslip lowered flat over the specimen without trapping air bubbles or letting stain overflow past its edges, and a microscope view appears showing at least a low-magnification field, with no action skipped, reordered, or performed with the wrong object.
level_5: All conditions for score 4 are met, AND the microscope view moves smoothly from low to high magnification within the same continuous shot, the high-magnification frame clearly resolves hyphae or spores in sharp, uniformly blue focus, no artifact (smearing, streaking, a drying edge, floating debris) obscures the specimen, and the slide stays centered with the coverslip aligned and the needle fully withdrawn before observation begins.

Scientific and Causal Correctness rubric:
level_1: The stain shown is not blue at all (e.g., colorless, yellow, red, or purple liquid), or the fungal specimen is either invisible or reduced to a shapeless blob with no hint of hyphae or spores, or nothing in the final microscope view shows any blue-stained structure.
level_2: The stain is blue but is shown as a solid powder, thick gel, or dry crystal rather than a spreadable liquid droplet, or the specimen shows no resolvable structure at all (just a dark speck with no visible branching or spore outline), or the microscope view shows blue color only at the coverslip's edge with none over the specimen itself.
level_3: The stain droplet is blue and liquid, and the specimen retains a recognizable basic shape (either a filamentous strand or a cluster of round bodies) after dispersion, though the blue staining is faint or patchy in places and the low-magnification microscope view is somewhat blurred or low-contrast.
level_4: All conditions for score 3 are met, AND the specimen is evenly dispersed with clearly resolvable branching hyphae or distinct spore/conidial shapes, the blue color penetrates the specimen uniformly rather than sitting only on its surface, and the microscope view, at both low and high magnification, shows the fungal structures in sharp focus with good contrast against the pale background.
level_5: All conditions for score 4 are met, AND the blue staining is equally intense across every visible hyphal strand and spore with no faded or unstained patches, the high-magnification view resolves fine diagnostic detail (e.g., septa dividing the hyphae, or a branching conidiophore), the color stays a consistent blue with no drift toward green or gray, and the specimen's shape and position hold steady with no shrinking, swelling, or dissolving during observation.

Spatiotemporal Consistency rubric:
level_1: The slide, needle, stain droplet, or coverslip appears or disappears abruptly with no motion connecting the two states (e.g., the needle is suddenly touching the specimen with no visible approach), or the view cuts hard from one microscope magnification to another with no zoom or pan connecting them, or the whole scene's brightness jumps sharply between one frame and the next with no visible cause, or the coverslip flips or spins into place in an impossible way with no hand or tool moving it.
level_2: The slide jumps sideways by a large, sudden distance (more than about half its width) between shots, or the needle moves toward the specimen with no visible hand or holder guiding it, or the stain droplet's size changes suddenly (e.g., small in one frame, covering half the slide in the next) instead of spreading gradually, or the coverslip drops onto the slide in a single instantaneous snap rather than a visible descent.
level_3: The slide holds still while the needle approaches and only wobbles slightly rather than staying perfectly fixed; the stain droplet's edge briefly flattens in an odd, non-physical way rather than spreading smoothly; the coverslip settles onto the slide with one small visible wobble before coming to rest rather than landing perfectly still; the microscope's focus changes in one visible step rather than a smooth rack, though no object disappears or breaks apart.
level_4: All conditions for score 3 are met, AND the needle's approach to the specimen is a single steady, continuous motion; the stain droplet spreads gradually and stops on its own without further change; the coverslip lowers and settles with a visible, gradual deceleration rather than any wobble; the microscope view zooms and re-centers smoothly between magnifications with no jump cuts.
level_5: All conditions for score 4 are met, AND the slide's position on the stage never shifts once the needle has withdrawn, the needle is fully removed from the frame before the coverslip is placed, the coverslip stays flat and parallel to the slide for the rest of the clip, and the microscope view keeps the specimen centered throughout the entire low-to-high magnification transition with no drift, blur, or ghosting at any frame.