Domain: Natural Science — Ecology & Evolutionary Biology
Target concept: Halophilic Artemia that are actively swimming contrast with non-motile Artemia in freshwater.

Video generation prompt: Two transparent bowls are placed side by side, one containing saltwater and the other freshwater; live brine shrimp are simultaneously introduced into both bowls.

Evaluation guide:
(i) Target concept: Osmoregulatory dependence of halophilic Artemia on environmental salinity determines motility capability.
Minimum mechanism: Artemia possess salt-dependent ion channels and contractile proteins; freshwater exposure triggers rapid depolarization failure and muscular incapacitation, not death; motility loss is immediate and reversible only upon return to saline medium.
(ii)Storyline: [Frame 0] Two transparent bowls enter static side-by-side frame; [Frame 1–2] Liquid fills each bowl (visually distinguishable via meniscus stability and surface reflectivity); [Frame 3] Live Artemia introduced synchronously into both bowls; [Frame 4–10] Sustained contrast emerges: Artemia in left bowl initiate and maintain coordinated swimming motion; Artemia in right bowl settle and remain fully stationary: no swimming initiation, no recovery, no drift-induced motion; viewpoint remains fixed, front-facing, level with liquid surface; all events occur within single continuous shot without cuts or repositioning.

Manual:
Experimental Procedure
1. Place two identical transparent bowls side by side on a flat surface.
2. Fill left bowl with saturated saltwater; right bowl with dechlorinated freshwater.
3. Simultaneously introduce live halophilic Artemia (brine shrimp) into both bowls.
Observation of Experimental Phenomena
Artemia swim actively and continuously in the saltwater bowl; Artemia remain motionless or sink to the bottom in the freshwater bowl.
Summary of Experimental Principles
Halophilic Artemia require high salinity for neuromuscular function and osmoregulation; in freshwater, ion imbalance causes paralysis and loss of motility.

Prompt Grounding rubric:
level_1: No two transparent bowls appear; no liquid is visible in either bowl; no brine shrimp appear in either bowl; the bowls are not shown side by side.
level_2: Two transparent bowls are present and side by side, but at least one bowl has no liquid, or liquid is present in only one, or brine shrimp appear in only one bowl, or brine shrimp are absent from both bowls.
level_3: Two transparent bowls sit side by side, each holding a clear liquid, with live brine shrimp introduced into both at the same time; no other animals or unrelated objects appear in either bowl; the two bowls are clearly separate, distinguishable containers.
level_4: All elements from 3-score are present, and the two bowls are matched in shape, size, and transparency so that any behavioral contrast between them is attributable to the shrimp rather than to the containers; brine shrimp are visibly present and countable in both bowls at the start of the clip; neither bowl's liquid visibly differs in color or cloudiness beyond ordinary water clarity (the saltwater/freshwater difference is a setup fact, not a required visual cue).
level_5: All elements from 4-score are present, and the side-by-side placement, fixed camera framing, and simultaneous shrimp introduction are unambiguous throughout the full clip; no shrimp are added, removed, or edited in or out at any point after the initial introduction; nothing (hands, tools, labels) obstructs the view of either bowl for the remainder of the clip.

Scientific and Causal Correctness rubric:
level_1: There is no visible difference in movement between the two bowls: all shrimp sit still in both bowls, or all shrimp swim briskly in both bowls, or shrimp vanish from one bowl partway through the clip with no explanation.
level_2: Shrimp move in only one bowl but not in a way consistent with the salinity setup, or shrimp move in both bowls with the same speed and pattern, or shrimp in both bowls only drift passively with no self-propelled swimming, so no clear motility contrast is established.
level_3: Shrimp in the saltwater bowl swim actively while shrimp in the freshwater bowl are motionless or settled on the bottom; the two bowls show clearly different motility states; no shrimp cross between bowls.
level_4: All elements from 3-score are present, and the saltwater bowl's shrimp show sustained, coordinated swimming (steady directional or undulating movement) rather than brief twitching, while the freshwater bowl's shrimp stay fully still on the bottom or suspended with no propulsion at any point.
level_5: All elements from 4-score are present, and the motility contrast holds for the entire clip: no freshwater shrimp begins swimming at any point, and the saltwater shrimp's swimming does not stop for an extended stretch; all movement in the saltwater bowl looks self-propelled rather than caused by drifting or disturbance.

Spatiotemporal Consistency rubric:
level_1: One or both bowls jump to a new position or angle between frames; brine shrimp vanish from a bowl or appear suddenly without swimming in; the lighting flips on or off or changes abruptly; a bowl's rim warps or flickers; the liquid surface jumps to a different level with no visible cause.
level_2: One bowl visibly rotates, tilts, or slides relative to the other; a shrimp's movement looks rigid or jump-cut rather than swimming (e.g., snapping from one spot to another); the liquid surface stays perfectly flat even while a shrimp swims vigorously beneath it, or ripples appear with no shrimp nearby to cause them; a shadow's position doesn't match where the light appears to come from.
level_3: Both bowls stay in the same position and orientation relative to each other throughout; shrimp move smoothly within their own bowl with no limbs vanishing mid-motion; each liquid surface holds a stable, natural curve at the bowl wall; the direction and strength of the lighting look the same from the first frame to the last.
level_4: All elements from 3-score are present, and shrimp movement looks physically sensible: no sudden bursts of speed without cause, no shrimp passing through the bowl wall, no shrimp floating above the liquid surface, and shrimp do not pass through one another; any surface ripple lines up with a shrimp actually touching the surface.
level_5: All elements from 4-score are present, and the motion of every shrimp reads as continuous swimming with no stutter, repeated frames, or sudden freezing; appendage movement follows a natural swimming rhythm; the transparent bowls let the viewer clearly see shrimp at any depth without them seeming to vanish behind glare or murk.