Domain: Natural Science — Microbiology
Target concept: Fluorescent NAAT reactions gradually become brighter over time under blue light/ultraviolet light.

Video generation prompt: On the bench, two transparent microcentrifuge tubes are placed in a heating module under blue/UVC light: the left tube contains a fluorescent NAAT reaction mixture with the target nucleic acid, and the right tube is a no-target control; incubation begins.

Evaluation guide:
(i) Target concept: Time-resolved fluorescent detection of nucleic acid amplification in NAATs.
Minimum mechanism: Fluorescent dye (e.g., SYBR Green) intercalates into double-stranded DNA; amplification generates more dsDNA product over time; increased dye binding leads to proportional increase in emitted fluorescence under appropriate excitation wavelength (blue/UVC); no-target control lacks amplifiable template, so no dsDNA accumulation and no fluorescence increase.
(ii)Storyline: Frame 0: Static wide shot showing heating module with two identical transparent tubes placed left-right under blue/UVC light; no fluorescence visible. → Gradual emergence of cyan/green fluorescence exclusively inside left tube, intensifying continuously across frames. → Right tube remains visually identical to initial state (no emission, ambient-lit only). → Entire sequence observed from fixed frontal viewpoint; no camera motion, no object relocation, no lighting shift. → Fluorescence must originate from within tube volume, not surface or surroundings.

Manual:
Experimental Procedure
1. Place two transparent microcentrifuge tubes side-by-side on a heating module.
2. Illuminate both tubes uniformly with blue/UVC light.
3. Left tube: fluorescent NAAT reaction mixture with target nucleic acid. Right tube: no-target control.
4. Begin incubation — tubes remain stationary under light.
Observation of Experimental Phenomena
Left tube gradually increases in fluorescence brightness (cyan/green) over time; right tube remains non-fluorescent (no emission) throughout.
Summary of Experimental Principles
Fluorescent NAAT reactions emit increasing fluorescence upon target amplification; under blue/UVC excitation, dye-bound dsDNA fluoresces progressively as amplicons accumulate — a time-dependent, target-specific signal.

Prompt Grounding rubric:
level_1: No microcentrifuge tubes are visible, or only one tube is shown, or the tubes are not placed on a heating module, or no blue/UVC light source illuminates them, or the two tubes are not arranged side by side, or nothing marks the start of incubation (e.g., tubes are never shown settled in place).
level_2: Two transparent microcentrifuge tubes appear side by side, but at least one of the following is missing: placement on a heating module; a visible blue/UVC light illuminating both tubes; a clear left/right spatial arrangement that lets a viewer tell the two tubes apart; or any cue that incubation has begun (e.g., the tubes are still being handled rather than resting in place).
level_3: Two transparent microcentrifuge tubes sit side by side on a heating module under visible blue/UVC light, in a clear left-right arrangement; incubation's start is shown by the two tubes resting still and undisturbed, with no hands or tools still moving them; no other setup element is required at this level.
level_4: Everything in the 3-point description holds, and in addition the two tubes are identifiable as playing different roles (the left tube as the reaction containing the target nucleic acid, the right tube as the no-target control) through a consistent visual cue such as a label, on-screen text, or an established left/right convention carried from the setup; the heating module also shows a visible sign of being switched on, such as an indicator light or a warm-toned surface glow.
level_5: Everything in the 4-point description holds, and in addition both tubes stay completely still with no shaking or jitter; the blue/UVC light reaches both tubes evenly with no obvious dark patch on either one; the left/right identification stays legible for the whole clip because the camera never repositions or zooms; and the heating module's surface looks like one continuous, well-fitted unit with no visible gap or misalignment where the tubes sit.

Scientific and Causal Correctness rubric:
level_1: No fluorescence appears in either tube; or the right (no-target) tube glows before or at the same time as the left tube; or the left tube's glow appears instantly at the very first frame instead of building up; or the glow is the wrong color for a fluorescent dye under blue/UVC light (e.g., white, yellow, or a plain reflection instead of cyan/green); or neither tube's brightness changes over time.
level_2: Only the left tube glows, but its brightness stays constant instead of climbing over time; or the left tube glows the wrong color for a typical NAAT dye under blue/UVC excitation (e.g., red, purple, or plain blue instead of cyan/green); or the right tube's brightness increases at any point.
level_3: Only the left tube glows, and its brightness climbs visibly over the course of the video, going from dark to noticeably brighter; the glow color reads as cyan, green, or yellow-green, consistent with common NAAT dyes under blue/UVC light; the right tube stays dark or shows only plain ambient light with no glow and no brightness change.
level_4: Everything in the 3-point description holds, and in addition the left tube's brightening is smooth and continuous with no flicker, sudden jump, or stall; the right tube shows absolutely no glow, halo, or stray light bleeding around it; and the glow in the left tube stays contained within the tube itself rather than spreading onto the heating module or background.
level_5: Everything in the 4-point description holds, and in addition the left tube's brightening rate looks gradual and steady rather than sudden or accelerated; the glow is not visible right from the first frame: it clearly starts partway through the clip, showing that the reaction takes time to build up; and neither tube shows a false glow from reflections, lens flare, or specular highlights that could be mistaken for real fluorescence.

Spatiotemporal Consistency rubric:
level_1: A tube appears or disappears abruptly instead of staying in view; or a tube's position or orientation changes with no visible motion carrying it there; or the lighting switches on and off discontinuously; or the heating module's surface cracks, warps, or shows an impossible shape; or a tube's material appears to deform, melt, or change transparency for a reason unrelated to the fluorescence itself.
level_2: One clear spatiotemporal break appears: a tube jumps to a new position between frames; the light flickers off completely more than once; the tubes' shadows swing to a new direction with no change in the light source; a tube rotates with no physical cause; or the background shifts suddenly while the camera itself stays still.
level_3: Neither tube teleports, warps, or deforms; the heating module's surface and shape stay consistent; no sudden lighting jump or shadow reversal occurs; no tube's transparency changes in an unnatural way; each tube stays exactly where it started relative to the module and to the other tube throughout the clip; any motion present looks smooth and physically plausible.
level_4: Everything in the 3-point description holds, and in addition the lighting stays spatially consistent (shadows keep the same direction and there's no doubled shadow); each tube's liquid surface looks stable frame to frame with no ghosting or duplication; and the heating module's surface texture looks uniform throughout, without abruptly switching to a different material look.
level_5: Everything in the 4-point description holds, and in addition there's no motion blur inconsistent with how the camera appears to be shooting; no frame is duplicated or skipped; the tube walls show no visible parallax mismatch against the background; and the left and right tubes stay in clearly the same positions relative to each other for the entire clip, with no visible drift of either tube out of alignment.