Domain: Healthcare — Pathology
Target concept: Repair (Old Scar)

Video generation prompt: An old scar tissue section is placed on the experimental table, and the fibrous structure is observed under a light microscope.

Evaluation guide:
(i) Target concept: Visualization of mature collagen architecture in an old scar via light microscopy.
Minimum mechanism: Scar tissue must be histologically processed (paraffin-embedded, H&E-stained), mounted on a glass slide, and placed on a microscope stage; light transmission through thin section enables visualization of collagen density, organization, and cellular absence.
(ii)Storyline: [Phase 1: Setup] Scar tissue section appears stationary on table surface → [Phase 2: Instrumentation] Microscope is statically positioned with objective centered over tissue → [Phase 3: Observation] Microscope eyepiece view transitions (or is shown) to reveal fibrous collagen pattern → [Causal transition] Placement + alignment → optical path completion → structured light transmission → fibrous pattern visibility → [Perspective constraint] Fixed overhead/side-on viewpoint showing table-tissue-microscope triad; no first-person eye view or internal microscope POV unless explicitly framed as eyepiece view; [Visibility constraint] Fibrous structure must be clearly resolvable (not blurred, not out-of-focus, not occluded) in final observed frame.

Manual:
Experimental Procedure
1. Place an old scar tissue section on the center of the experimental table.
2. Position a light microscope directly above the tissue with objective lens aligned vertically.
3. Look through the eyepiece to observe the tissue section.
Observation of Experimental Phenomena
Under the light microscope, dense, acellular, wavy-to-parallel collagen fiber bundles appear as pale pink, homogeneous cords against a faintly eosinophilic background — with no nuclei, vessels, or inflammation.
Summary of Experimental Principles
Old scar tissue consists of mature, avascular collagen deposited during fibrosis; its fibrous architecture replaces original tissue architecture and lacks cellular components, distinguishing it from granulation tissue or normal dermis.

Prompt Grounding rubric:
level_1: No old scar tissue section is shown; no experimental table appears; no light microscope appears; none of placing the tissue, positioning the microscope, or observing it happens; none of the three required elements (tissue, table, microscope) appear together.
level_2: At least one required element is present (e.g. some tissue sample or a microscope) but the old scar section itself is missing, or the table and microscope don't appear together in a way that relates to each other; no placement or observation action ever happens.
level_3: An old scar tissue section sits on an experimental table, a light microscope is present in the same scene, and its objective lens or eyepiece is oriented toward the tissue; however, there's no visible sign that anyone is actively looking through it (e.g. no eye positioned at the eyepiece, no in-focus view shown).
level_4: The old scar section sits centered on the table; the microscope is positioned directly above or beside it with its objective lens aligned over the tissue; the full microscope body and base are visible; there's no unrelated object (gloves, stray labels, other slides) interfering with this core setup.
level_5: Everything required at the 4-point level holds, and in addition: the microscope stays fixed in place with no motion blur or repositioning for the whole clip; the tissue section doesn't move on the table; the only camera transition allowed is the single cut or zoom into the eyepiece view that reveals the fibrous structure, with no other camera cut, zoom, or pan; the tissue and microscope keep the same spatial relationship to each other throughout, with neither one blocking the view of the other.

Scientific and Causal Correctness rubric:
level_1: The tissue looks like fresh wound tissue, granulation tissue, or a non-fibrous tissue type (e.g. fat, muscle, or surface epithelium) instead of scar; no cord-like fibrous structures are visible at any zoom level; the tissue's color doesn't match mature scar (e.g. red/pink like fresh tissue instead of the pale, washed-out look of old scar); there's no visible contrast between fiber bundles and the surrounding tissue.
level_2: The tissue is presented as if it were scar, but the microscope view shows no real fibrous pattern: the slide looks blank, is out of focus, or instead shows something unrelated (e.g. cells caught mid-division, or clusters of inflammatory cells); no cord-like collagen pattern is visible anywhere in the field.
level_3: The microscope view shows pale, cell-free, cord-like bundles running largely alongside one another, matching mature collagen in an old scar; the background is evenly pink-stained; the field contains no visible nuclei, blood vessels, or signs of inflammation.
level_4: Everything required at the 3-point level holds, and in addition: the pale parallel collagen cords fill more than half of the visible field; there's no mixture of younger-looking collagen (which would look wavier, bluer-tinted, or more cellular); no hair follicles, glands, or other skin structures remain in the field; no elastic-fiber network is visible; the pink staining looks uniform across the whole field, with no blue nuclear staining anywhere.
level_5: Everything required at the 4-point level holds, and in addition: the collagen bundles look even more uniformly thick, densely packed, and aligned together across the entire field — the hallmark tightly packed, largely parallel look of a mature old scar, clearly different from the looser, basket-weave crisscrossing pattern of ordinary skin; there are no leftover cell nuclei, no blood vessels, and no pigment or hemosiderin deposits anywhere; there's no processing artifact (no knife marks, folds, or tearing) breaking up the fibers; the entire field is made up of this mature collagen with no adjacent normal or inflamed tissue visible.

Spatiotemporal Consistency rubric:
level_1: The tissue section jumps to a different location on the table; the microscope vanishes and reappears; the scene's brightness suddenly swings from fully lit to dark (or the reverse) mid-clip; something structurally breaks (e.g. the tissue splits apart, the table appears to crack, or the lens detaches); the tissue or microscope moves in a way that isn't physically possible (e.g. the tissue floats, or the microscope rotates 90° with nothing supporting it).
level_2: One clear spatiotemporal problem occurs: the tissue is shown already placed on the table with no placement action ever shown; or the microscope's position changes abruptly between two different angles with no transition; or the shadow it casts swings to a different side even though nothing shows the light source moving; or the focus jumps from sharp to fully blurred and back with no gradual change in between.
level_3: The tissue and microscope each stay in the same place relative to each other and to the table throughout; nothing vanishes, jumps, or breaks apart; the shadow the microscope casts stays on the same side and at roughly the same strength across the clip; the tissue lies flat on the table with no unnatural warping or floating; the microscope stays steady, without unexplained shaking.
level_4: Everything required at the 3-point level holds, and in addition: the camera itself stays completely still (no pan, tilt, zoom, or dolly); there's no flicker or strobing between frames; a hand placing the tissue is never shown at the same time the tissue is already sitting in place; the edges of the table and tissue keep a consistent relationship to each other as the view is held.
level_5: Everything required at the 4-point level holds, and in addition: there's no motion blur on objects that should be perfectly still; no interlacing or compression blockiness is visible; no ghosting trails appear behind any object; every frame shows the tissue, table, and microscope continuously and solidly present, with no repeated identical frames that would suggest a freeze or a looped clip.