Domain: Healthcare — Pathology
Target concept: Granulation Tissue

Video generation prompt: A tissue repair phase granulation tissue section is placed on the experimental table, and the tissue structure is observed under the microscope.

Evaluation guide:
(i) Target concept: Visual identification of histopathological granulation tissue as the hallmark of active wound repair.
Minimum mechanism: Tissue must be thin-sectioned and routinely stained (e.g., hematoxylin and eosin) to reveal cellular and stromal detail, mounted on glass, and imaged under standard brightfield microscopy; no immunohistochemistry, fluorescence, or electron microscopy required; capillary loops must be biologically plausible (thin-walled, looping, luminal patency implied).
(ii)Storyline: [Phase 1: Macro setup] Tissue section rests on clean experimental table → [Phase 2: Instrument integration] Slide is placed onto microscope stage with alignment visible → [Phase 3: Optical engagement] Microscope eyepiece view activates, revealing focused field → [Phase 4: Structural revelation] Capillary loops emerge first at periphery, then fibroblasts and stroma resolve centrally → [Perspective constraint] Fixed overhead or front-oblique angle for macro; strictly centered, eye-level optical axis for micro; no cutaways, no narrator hand, no annotations.

Manual:
Experimental Procedure
1. Place granulation tissue section on glass slide.
2. Position slide on microscope stage.
3. Observe tissue structure through microscope eyepiece.
Observation of Experimental Phenomena
Moist, red-pink tissue with irregular thin-walled capillary loops projecting vertically into loose connective stroma containing scattered plump fibroblasts and sparse inflammatory cells.
Summary of Experimental Principles
Granulation tissue is the provisional connective tissue formed during wound healing, characterized by new capillary formation (angiogenesis), fibroblast proliferation, and edematous extracellular matrix — marking the active tissue repair phase.

Prompt Grounding rubric:
level_1: The experimental table is empty or holds unrelated items (e.g., blood collection tubes, scalpels, a slide from a different tissue); no granulation tissue section, no microscope, and no glass slide appear anywhere in frame.
level_2: An experimental table and a microscope are both visible, but the tissue on the slide is clearly not granulation tissue (e.g., smooth pale skin, dense white scar tissue, or gray necrotic debris), or a slide sits beside the microscope rather than seated on its stage, or no tissue is mounted on the slide at all.
level_3: A granulation tissue section is mounted on a glass slide and seated squarely on the microscope stage, held by the stage clips, with the objective lens lowered toward it; the eyepiece and objective turret are both visible; nothing beyond this placement (no focusing, no eyepiece view) has yet occurred.
level_4: Everything in score 3 holds, and the shot additionally moves from the tabletop framing of the slide-on-stage into the eyepiece's own field of view, with the objective lens now in focus and the substage or external light source visibly illuminating the specimen.
level_5: Everything in score 4 holds, and the eyepiece field shows only the granulation tissue section (centered, in focus, and free of any timestamp, arrow, circle, or other overlay), with no additional zooming, reframing, or stage repositioning once this view is reached; the same patch of tissue stays in view for the remainder of the shot.

Scientific and Causal Correctness rubric:
level_1: The tissue field is a single flat pink or red expanse with no visible microstructure (no distinguishable vessels, spindle cells, or nuclei), resembling homogeneous muscle or a blood clot smear rather than any organized stroma; or the section looks translucent/gray like adipose or necrotic tissue.
level_2: Vessels are present but are thick-walled and run in parallel, straight courses typical of mature arterioles/venules rather than looping capillaries, or the stroma is densely packed with pink collagen bundles and almost no spindle-shaped nuclei, an appearance closer to a mature, healed scar than to active granulation tissue.
level_3: The field shows the expected granulation-tissue picture: eosin-pink stroma studded with thin-walled capillary loops that bend back on themselves (rather than running straight), scattered spindle-shaped fibroblast nuclei (hematoxylin-purple) among the loose stroma, and occasional inflammatory cells; there is no keratinized surface layer and no intact basement membrane anywhere in the field.
level_4: Everything in score 3 holds, and additionally the capillary loops point their long axis toward the tissue surface rather than lying flat or at an angle, the fibroblast nuclei are elongated and lined up roughly perpendicular to that same surface, and the stroma between the vessels looks loose and pale/edematous rather than packed with dense pink collagen bundles.
level_5: Everything in score 4 holds, and the field is free of anything that would contradict a textbook granulation-tissue diagnosis: no endothelial or fibroblast nuclei are caught mid-division, no clusters of epithelial cells sit within the stroma, no suture fragment or other foreign material appears, no golden-brown hemosiderin pigment is deposited, and no pooled blood obscures the capillary and fibroblast pattern.

Spatiotemporal Consistency rubric:
level_1: The shot cuts abruptly between the tabletop view and the eyepiece view with no transition; the microscope stage or the slide disappears between frames and reappears in a different spot; the scene's overall illumination flips abruptly from dark to bright (or bright to dark) between frames with no dimmer switch or light-source change to explain it; or the tissue's pink-red color swaps to an unrelated hue (e.g., blue or green) from one frame to the next with no such stain change occurring in the procedure.
level_2: The slide or tissue section jumps sideways or vertically by more than its own width between one moment and the next without any camera or hand motion to account for it; the objective lens or eyepiece barrel visibly stretches or bends out of its normal cylindrical shape; or the capillary loops and fibroblasts in focus in one frame become a completely different, unrelated pattern in the next without any stage or focus adjustment shown.
level_3: The tissue section stays in the same spot on the slide, and the slide stays seated on the stage, for the whole sequence; the pink-red color of the stroma and the brightness of the capillary-loop highlights under the eyepiece's own illumination look about the same from the first moment the field comes into focus to the last; the slide never disappears, jumps, or swaps with a different specimen.
level_4: Everything in score 3 holds, and the move from the tabletop framing into the eyepiece field happens as one continuous camera move or optical zoom rather than a hard cut; the stage clips, cover slip edge, and objective lens keep believable relative sizes and correct occlusion (e.g., the cover slip edge stays behind the objective as the lens passes over it) throughout the move.
level_5: Everything in score 4 holds, and across the whole eyepiece-view segment the capillary loops and fibroblast nuclei keep the same relative spacing and brightness frame to frame: no flicker in the highlight on a capillary loop, no shadow separating from the tissue edge, and no moment where the focus looks sharp then soft then sharp again without a corresponding hand or stage movement to explain it.