Domain: Natural Science — Biochemistry & Molecular Biology
Target concept: Thermophilic catalase produces oxygen bubbles after heat treatment, compared to n.

Video generation prompt: Two adjacent transparent beakers: red heat-stable catalase and blue regular catalase are heated in a water bath, followed by the simultaneous addition of hydrogen peroxide.

Evaluation guide:
(i) Target concept: Enzyme thermostability determines catalytic capacity under heat stress.
Minimum mechanism: Heat-stable catalase must remain folded and active post-heating to rapidly cleave H₂O₂ → O₂(g) + H₂O(l), while heat-labile catalase must be irreversibly denatured and inert under identical conditions.
(ii)Storyline: Frame 1–2: Static wide shot showing two adjacent beakers in water bath, red solution in left, blue in right. Frame 3–4: Water bath visibly quiescent, no steam or boiling, confirming controlled heating (not boiling). Frame 5: Dual dropper or synchronized twin application delivers H₂O₂ to both beaker surfaces in same instant. Frame 6–10: Immediate, exclusive bubble nucleation and ascent in red beaker; blue beaker surface remains optically flat and motionless: no effervescence, no meniscus disturbance, no micro-turbulence. Viewpoint remains fixed, frontal, and orthogonal to beaker row; no occlusion, no camera motion, no lighting shift.

Manual:
Experimental Procedure
1. Place two identical transparent beakers side-by-side in a water bath.
2. Fill left beaker with red heat-stable catalase solution; fill right beaker with blue regular catalase solution.
3. Heat both beakers in water bath until thermal equilibrium reached.
4. Simultaneously add hydrogen peroxide to both beakers.
Observation of Experimental Phenomena
Only the red beaker produces continuous, vigorous oxygen bubbles after hydrogen peroxide addition; the blue beaker shows no bubble formation.
Summary of Experimental Principles
Thermophilic catalase retains enzymatic activity after heat treatment and decomposes H₂O₂ into O₂ and H₂O, producing visible oxygen bubbles; regular catalase is denatured by heat and loses function, producing no bubbles.

Prompt Grounding rubric:
level_1: Neither a red catalase beaker nor a blue catalase beaker is shown; no water bath heating is depicted; no hydrogen peroxide addition event occurs; the two-beaker side-by-side setup described in the prompt is absent.
level_2: Two containers appear but are not recognizable as beakers (e.g., flasks or unlabeled vessels), or only one colored solution is shown, or the colors present don't match red/blue; the water bath is missing or replaced with an open flame; hydrogen peroxide is added before any heating takes place.
level_3: A red-solution beaker and a blue-solution beaker sit side by side, both visibly submerged in a water bath; hydrogen peroxide is added to both beakers, but with a noticeable time gap between the two additions rather than at essentially the same moment.
level_4: The red beaker and blue beaker are clearly side by side, each holding only its assigned color, both fully immersed in a calm, clear water bath; hydrogen peroxide is added to both beakers at essentially the same visual moment (e.g., two droppers releasing liquid together, or one dual applicator touching both surfaces together) rather than one after the other.
level_5: The red and blue beakers are the same size and shape, placed at the same height in a calm, clear water bath that submerges them to the same level; each beaker holds only its assigned color with no bleeding or mixing between them; hydrogen peroxide is released into both beakers at the same visible instant, with droplets of comparable size landing on both surfaces at essentially the same moment, all within one continuous, uninterrupted shot.

Scientific and Causal Correctness rubric:
level_1: No oxygen bubbles form in either beaker at any point in the video; both the red and blue catalase solutions stay visually static and bubble-free throughout.
level_2: Bubbles appear in only one beaker regardless of which color it is, or bubbles appear in both beakers with similar intensity and timing, or bubbles appear before the hydrogen peroxide is added, or before any heating occurs.
level_3: Bubbles form only after hydrogen peroxide is added, and appear in both beakers but start noticeably earlier in the red beaker than in the blue one; the blue beaker shows at most a brief, minor fizzle rather than sustained bubbling.
level_4: Bubbles form only after hydrogen peroxide addition and only in the red beaker; the blue beaker shows no rising bubbles, surface fizzing, or foam at any point; the red beaker's bubbling is continuous and vigorous, rising steadily from within the liquid; the water-bath heating context established earlier in the video is still visually intact when the hydrogen peroxide is added (e.g., the beakers have not been shown being removed or cooled).
level_5: Bubbles form exclusively and continuously in the red beaker beginning right when the hydrogen peroxide contacts it, with no noticeable delay; the blue beaker stays completely free of bubbles, surface disturbance, or foam for the entire video; the red beaker's bubbles are distinct, roughly spherical, and rise steadily through the clear liquid, while the blue beaker shows no bubble-like artifacts (floating specks, glare, sediment movement) that could be mistaken for gas evolution.

Spatiotemporal Consistency rubric:
level_1: The beakers or their contents jump abruptly between frames with no visible transition; the scene's overall brightness switches sharply (e.g., bright to dark) with no dimming/brightening transition; a beaker's shape visibly warps between frames; the liquid level in a beaker changes suddenly with no depicted cause; the camera view jumps from one framing to another (e.g., close-up to wide shot) with no cut or pan shown.
level_2: One of the two beakers vanishes from view and later reappears; a solution's color shifts abruptly (e.g., red turning purple) with no mixing event shown to explain it; the hydrogen peroxide dropper appears or disappears with no approach or withdrawal motion; bubbles begin forming with no visible droplet ever reaching the liquid surface.
level_3: Both beakers stay in the same place, shape, and size throughout; the overall lighting stays even; the liquid surface in both beakers is calm and undisturbed before the hydrogen peroxide is added; the dropper's approach toward each beaker is visible and continuous; once a droplet contacts the red beaker's surface, bubbles begin forming with no perceptible delay; neither beaker's walls nor liquid surface show any structural break.
level_4: Both beakers keep the same fixed spacing and alignment relative to each other throughout, with no wobble or shift in perspective; the dropper (or pair of droppers) approaches both beakers along matching paths at matching speed; once bubbling starts in the red beaker, the bubbles rise along a consistent, roughly vertical path from frame to frame; the blue beaker's liquid surface stays completely calm and undisturbed for the entire sequence.
level_5: Every element of motion is smooth and physically coherent throughout: no jitter between frames, no motion blur that mismatches how fast an object is actually moving, no inconsistency in how the beakers occlude or reveal each other or the background, and no mismatch between a beaker and its reflection on its own surface; the droplet's fall, its contact with the liquid, the onset of bubbling, and the bubbles' rise all play out as one continuous, physically plausible sequence with no visible interpolation glitches or stutters.