Domain: Healthcare — Pharmacy
Target concept: Antagonism of Anticoagulant Drugs by Vitamin K

Video generation prompt: Two identical transparent containers are placed side by side. The left container holds a blood plasma sample from a patient whose anticoagulant therapy has already been reversed by vitamin K, combined with a clotting activator reagent; the right container holds a blood plasma sample from a patient still under anticoagulant therapy without vitamin K restoration, combined with the same clotting activator reagent. The clotting reaction is observed.

Evaluation guide:
(i) Target concept: A clotting-time assay reveals that vitamin K restores functional, clotting-competent factors after anticoagulant therapy, while plasma still under anticoagulant therapy remains clotting-deficient.
Minimum mechanism: Vitamin K, once administered in vivo, serves as the cofactor recycled by VKORC1 for the hepatic enzyme γ-glutamyl carboxylase, enabling γ-carboxylation of clotting factors II, VII, IX, and X over hours to days; a plasma sample drawn after this restoration contains functional clotting factors, while a plasma sample from a patient still under anticoagulant therapy (without vitamin K reversal) contains under-carboxylated, non-functional factors. Adding a shared calcium/tissue-factor clotting activator to both plasma samples triggers clot formation only in the vitamin K-restored plasma within the assay window; the still-anticoagulated plasma shows delayed or absent clotting.
(ii)Storyline: Frame opens on a static frontal view of two identical transparent containers side-by-side → left container holds vitamin K-restored plasma, right container holds anticoagulated plasma (both visually similar, pale yellow) → the same clotting activator reagent is added to both → left container progressively develops a visible clot (a solid or gel-like mass) within the assay window while right container remains liquid → the contrast is maintained and observable for ≥2 seconds → no intervention, labeling, or narration required; all causality must be inferable from the visual state difference alone.

Manual:
Experimental Procedure
1. Place two identical transparent containers side-by-side.
2. Fill the left container with a plasma sample from a patient whose anticoagulant therapy has already been reversed by vitamin K administration (functional clotting factors restored); add the clotting activator reagent (e.g., calcium chloride with tissue factor).
3. Fill the right container with a plasma sample from a patient still under anticoagulant therapy without vitamin K restoration (clotting factors remain under-carboxylated and non-functional); add the same clotting activator reagent.
4. Observe clot formation over the following seconds to minutes, as in a standard prothrombin-time-style assay.
Observation of Experimental Phenomena
The left container (vitamin K-restored plasma) forms a visible clot within the assay window; the right container (still-anticoagulated plasma) shows delayed or absent clot formation and remains liquid over the same window.
Summary of Experimental Principles
Vitamin K restores functional, γ-carboxylated clotting factors (II, VII, IX, X) by acting as the cofactor for the liver enzyme γ-glutamyl carboxylase, recycled via VKORC1 — this hepatic synthesis process takes hours to days in vivo, not an instant reaction in a container. Once functional clotting factors are present, plasma clots within seconds of adding a calcium/tissue-factor activator, as in a standard prothrombin time test; plasma still lacking functional factors shows prolonged or absent clotting under the same activator. This demonstration depicts that downstream clotting-activator assay, which reflects each plasma sample's factor status rather than showing the hours-to-days hepatic synthesis process itself.

Prompt Grounding rubric:
level_1: No two transparent containers are shown side by side; or only one container appears; or the left container does not visibly receive both a plasma sample and the clotting activator reagent; or the right container does not visibly receive both a plasma sample and the same clotting activator reagent; or either plasma sample is missing entirely.
level_2: Two transparent containers appear side by side, but at least one of the following is wrong: the left container's plasma sample or activator reagent is missing, the right container's plasma sample or activator reagent is missing, or the two containers visibly receive different activator reagents, breaking the controlled comparison.
level_3: Two transparent containers sit side by side; each one visibly receives a plasma sample and the same clotting activator reagent; no unrelated object (label, extra glassware, hand) dominates the frame.
level_4: Everything required at the 3-point level is present, and both containers keep the same transparency, shape, and size, with no occlusion or partial framing of either container; the moment the activator reagent enters each container is visible for both.
level_5: Everything required at the 4-point level is present, and the two containers keep a visible, unbroken gap between them, both stand upright at the same angle, and the camera holds a fixed frontal framing showing the full height and width of both containers for the entire clip.

Scientific and Causal Correctness rubric:
level_1: No clot forms in either container; or both containers reach the same end state; or a clot appears in the right (still-anticoagulated) container instead of the left; or a clot appears before the activator reagent is added to that container.
level_2: A clot forms in one container, but the pattern is wrong: e.g., the clot forms in the right container instead of the left, or both containers end up looking the same, or the state change happens in a container before its activator reagent is visibly present.
level_3: The left container (vitamin K-restored plasma) develops a visible clot (a solid or semi-solid mass, loss of liquid homogeneity, or a distinct opaque clump) within the assay window, while the right container (still-anticoagulated plasma) stays a clear, uniform liquid with no clumping; this contrast holds for at least 2 seconds within the clip.
level_4: Everything required at the 3-point level is present, and the clot in the left container does not re-dissolve or come and go, while the right container stays liquid throughout; no bubble or floating sediment in either container is mistaken for a clot.
level_5: Everything required at the 4-point level is present, and the left container shows one continuous, unambiguous clot mass with no remaining free-flowing liquid layer, while the right container shows no solid material anywhere in its volume and keeps a flat, undisturbed liquid surface throughout.

Spatiotemporal Consistency rubric:
level_1: One or both containers vanish, appear from nowhere, or change size abruptly between frames; the scene suddenly goes dark or over-bright with no visible cause; a container's outline bends or stretches out of its true shape; the camera view jumps to a steep top-down or side angle with no lead-in.
level_2: One container's position jumps to a new spot with no visible path connecting the two positions; light or shadow briefly hides more than half of either container's contents; a container's edge flickers or breaks apart across three or more consecutive frames.
level_3: Both containers stay fully visible, in place, and intact throughout; the lighting stays even enough to see the container walls and the liquid inside each; nothing (a hand, a label) suddenly blocks either container; the clip plays without dropped or frozen frames.
level_4: Everything required at the 3-point level is present, and any liquid motion (settling, gentle swirling from the activator being added) looks physically natural and occurs similarly in both containers rather than only one; any shadow keeps a consistent direction and darkness matching a single steady light source.
level_5: Everything required at the 4-point level is present, and the whole clip plays smoothly with no jerky cuts or repeated frames; each container's walls bend the background behind it the same way on the left and right container; the camera's viewing angle and distance to both containers stay constant with no drift.