Domain: Healthcare — Pathology
Target concept: Inflammatory tissue proliferation (chronic inflammation)

Video generation prompt: A histological section of a chronic inflammatory lesion is placed on the microscope stage for observation of histological changes.

Evaluation guide:
Target concept: Inflammatory tissue proliferation in chronic inflammation is defined as a coordinated, histologically identifiable triad: (1) persistent lymphoplasmacytic infiltration, (2) stromal activation (fibroblast proliferation + capillary neogenesis), and (3) epithelial/glandular hyperplasia, arising as an adaptive, reparative response to ongoing immune stimulation. Minimum mechanistic assumptions: (i) chronic inflammation persists long enough to trigger stromal remodeling (≥2–4 weeks), (ii) H&E staining reliably differentiates nuclear morphology (lymphocyte vs. plasma cell vs. fibroblast), cytoplasmic eosinophilia (collagen, glandular secretion), and vascular structure, and (iii) light microscopy in the 10×–40× range is sufficient to recognize the gross pattern of chronic inflammatory proliferation (a dense cluster of small inflammatory cells beside a thickened stromal band with small vessel- or gland-like structures) without requiring resolution of individual nuclear chromatin detail. Expected phenomena storyline: (Phase 1) Slide placement → (Phase 2) 10× survey revealing architectural distortion and inflammatory infiltrate → (Phase 3) Objective switch to higher power → (Phase 4) Simultaneous visualization of three co-localized proliferative elements: (a) plasma cells nestled among fibroblasts, (b) capillary sprouts adjacent to activated stroma, (c) hyperplastic glands overlying inflamed lamina propria, all within one stable, well-illuminated, in-focus field. Visibility constraints: Features should be visible in the 10×–40× range as a recognizable low-to-moderate-magnification pattern of cell clustering, stromal thickening, and small vessel/gland crowding; precise focus and even illumination are still required, but resolving individual nuclear chromatin patterns is not.

Manual:
I. Basic Demonstration Information
Demonstration Title: Histological Observation of Inflammatory Tissue Proliferation in Chronic Inflammation
Subject: Healthcare - Pathology - Inflammatory Tissue Proliferation (Chronic Inflammation)
Demonstration Purpose: To visually identify hallmark histological features of chronic inflammatory tissue proliferation—including lymphoplasmacytic infiltration, fibroblast activation, capillary neogenesis, and epithelial/glandular hyperplasia—using a standard H&E-stained tissue section under light microscopy; to distinguish proliferative repair from acute injury or neoplasia; and to master diagnostic pattern recognition based on spatial organization and cellular morphology.
II. Demonstration Carrier/Equipment
Core Carrier: H&E-stained paraffin section of a confirmed chronic inflammatory lesion (e.g., chronic active gastritis, rheumatoid synovium, or tuberculous granuloma with fibrosis)
Auxiliary Equipment: Compound light microscope (with 10× and 40× objectives), microscope stage with clip fixation, standardized brightfield illumination (Köhler alignment), digital eyepiece or camera (optional for annotation), label card reading ‘Chronic Inflammatory Lesion’.
III. Demonstration Content Description
Layout: Place labeled slide centered on microscope stage; secure with stage clips. Begin observation at 10× objective to survey architecture; switch to 40× for cellular detail. Maintain consistent illumination and focus depth.
State Control: Ensure section is fully deparaffinized and evenly stained—no folds, tears, or precipitate obscuring tissue. Avoid excessive light intensity that bleaches eosin or causes glare.
Comparison Baseline: Contrast with adjacent non-inflamed stroma or normal mucosa in same section to highlight proliferative changes—not absolute cell counts, but *relative increase* in fibroblasts, capillaries, and glands.
IV. Observation of Core Phenomena
Lymphoplasmacytic Infiltrate: Dense, organized aggregates of small lymphocytes (dark, round nuclei) and plasma cells (eccentric nuclei, clock-face chromatin, pale cytoplasm) in lamina propria or interstitium.
Fibroblast Proliferation: Spindle-shaped stromal cells with enlarged, vesicular nuclei and abundant pale-pink cytoplasm—aligned parallel to collagen, increasing cell density 2–3× over normal stroma.
Capillary Neogenesis: Small, thin-walled vessels with single-layer endothelium and minimal red blood cells—often clustered near inflammatory foci, with visible endothelial sprouts.
Epithelial/Glandular Hyperplasia: Crowded, stratified, or branching glands with uniform basal nuclei; increased mitotic figures rare but present; no cytologic atypia.
Characteristic Stability: All proliferative features co-localize spatially (e.g., capillaries adjacent to fibroblasts, glands overlying inflamed stroma); no isolated features—proliferation is a coordinated, tissue-level response.
V. Summary of Key Knowledge Points and Principles
Core Concept Definition: Inflammatory tissue proliferation is the adaptive stromal and parenchymal regenerative response to persistent inflammatory stimuli, characterized by fibroblast activation, angiogenesis, and epithelial hyperplasia—not mere cell accumulation, but *organized tissue rebuilding*.
Pathophysiological Significance:
• Fibroblast proliferation restores structural integrity via collagen deposition.
• Capillary neogenesis supports metabolic demand of inflamed/repairing tissue.
• Epithelial hyperplasia re-establishes barrier function and glandular output.
• Absence of these features suggests inadequate repair or immunosuppression; excess/uncontrolled proliferation may precede fibrosis or dysplasia.
VI. Demonstration Precautions
Slide Quality: Use only optimally fixed, processed, and stained sections—poor fixation causes nuclear pyknosis mimicking apoptosis; over-staining obscures plasma cell cytoplasm.
Magnification Fidelity: Always verify the objective label matches the visual field width (40× shows ~0.2 mm; 10× shows ~2 mm). Gross proliferative patterns — inflammatory-cell clustering, stromal thickening, and small vessel/gland crowding — are already recognizable at 10×; higher power (up to 40×) helps confirm fine detail but is not the only valid resolution for identifying proliferation.
Diagnostic Specificity: Distinguish proliferation from malignancy: chronic proliferative nuclei are uniform, lack pleomorphism or abnormal mitoses; stroma is organized, not desmoplastic or invasive.

Prompt Grounding rubric:
level_1: No microscope, slide, or tissue section appears at all; the scene shows something unrelated (e.g., a whole organ, a blood smear, or a blank stage) with no inflammatory features and no labeling.
level_2: A microscope is present but out of focus or empty, or a normal/healthy-looking tissue slide (e.g., unremarkable liver or muscle) sits on the stage with no sign of chronic inflammation; there is no label and no magnification cue of any kind.
level_3: A tissue section sits on the stage in roughly correct focus and basic structures (nuclei, pink-staining collagen) are visible, but the specific chronic-inflammation features (a cluster of small round inflammatory cells, thickened stroma, small vessel- or gland-like structures) are absent, unclear, or hard to identify; there is no magnification or orientation cue.
level_4: The tissue section is centered under the objective and the field shows a plausibly stained sample with a cluster of small round inflammatory cells and mild stromal thickening, but it stops short of clearly showing the proliferative elements themselves: no clearly thickened fibroblast-rich band, no small vessel-like channels, no crowded gland-like structures; the magnification looks like it could be 10×–40× but is never confirmed by a label.
level_5: The stage holds a properly mounted, pink-and-purple (H&E-style) stained section labeled 'Chronic Inflammatory Lesion'; the field is in sharp focus at a magnification in the 10×–40× range; the view clearly shows inflammatory-cell proliferation: a dense cluster of small round inflammatory cells together with a visibly thickened, cell-rich stromal band, and either small thin-walled vessel-like channels or crowded/piled-up gland-like structures; a magnification label (e.g., '40×') is visible on screen.

Scientific and Causal Correctness rubric:
level_1: No biological plausibility: the tissue looks necrotic, purely acute (a uniform sheet suggestive of neutrophils), or overtly malignant (disorganized, irregular masses), with no visible cluster of small round inflammatory cells, no thickened stroma, and no small-vessel- or gland-like structures; any apparent 'proliferation' is really just a fold, bubble, or stain artifact.
level_2: Inflammation is present but its chronic, tissue-building nature is misrepresented: the field looks dominated by a single, acute-looking cell population rather than a mixed cluster of small round cells, and the surrounding stroma looks flat and unremarkable rather than thickened; there is no visible cue of new tissue forming (no denser pink band, no small channel-like structures, no crowded gland pattern).
level_3: Some chronic-inflammation cues appear (a cluster of small round inflammatory cells is visible), but the tissue-rebuilding response is only weakly shown: the surrounding stroma looks only slightly denser than normal, with no clearly thickened band and no visible small-vessel-like channels, and any gland-like area looks evenly spaced rather than crowded; the link from 'inflammatory cluster' to 'tissue rebuilding' is not visually apparent.
level_4: All three gross hallmarks appear somewhere in the field (a dense cluster of small round inflammatory cells, a noticeably thickened, cell-rich pink-staining stromal band, and either small vessel-like channels or a crowded gland-like region), but they sit as separate patches rather than clearly joined together, so proliferation is shown without being visibly driven by the inflammatory cluster next to it.
level_5: The three hallmarks appear spatially and causally linked within one field: the dense inflammatory-cell cluster sits directly against the thickened stromal band, and that same band carries both small vessel-like channels and a crowded gland-like region, so a viewer can see the infiltrate, the stromal thickening, and the new vessel/gland structures forming together rather than as isolated patches; the overall purple-cell / pink-stroma color balance looks correct, with no area looking necrotic, acutely neutrophilic, or malignant.

Spatiotemporal Consistency rubric:
level_1: Severe spatiotemporal breakdown: the slide or stage seems to jump position between frames; the visible field's overall size changes abruptly with no zoom action shown; a cell cluster appears or disappears between frames with no focus or stage change to explain it; the image snaps from blurry to sharp with no visible focusing motion; or the illumination abruptly swings between bright and dim with no adjustment (e.g., condenser, light source) shown to explain it.
level_2: Moderate inconsistency: the slide looks already in focus before it's placed on the stage, or the field of view narrows (implying higher magnification) with no visible turret/objective rotation; the tissue's stain color or texture shifts abruptly between shots; or a vessel-like structure appears fully formed as if considerable time passed, with no corresponding action shown.
level_3: Minor gaps: a brief blurry moment while focus is adjusted; slight jitter during a focus-knob turn; one inconsistent moment such as a magnification label flickering or the background light changing once; the slide otherwise stays in place with a continuous field of view.
level_4: Motion stays physically plausible throughout: the slide is placed and then stays fixed on the stage; any magnification change is preceded by a visible turret rotation; the focus sweep moves gradually rather than snapping; illumination stays even; and the cell cluster, stromal band, and any vessel- or gland-like structures keep proportionate relative sizes across the clip.
level_5: Full spatiotemporal integrity: the slide never shifts on its own once placed; every magnification change is visibly driven by a turret rotation with a matching change in field-of-view width; the focus transition is one smooth, continuous sweep with no jump cuts; illumination stays even and glare-free throughout; and the cell cluster, stromal band, and vessel-/gland-like structures keep consistent relative size, shape, and position across frames, without warping, splitting, or drifting apart.