Domain: Healthcare — Pathophysiology
Target concept: Rabies

Video generation prompt: A brain tissue section from a rabies patient, stained with HE and Negri body staining, is placed on the experimental table for observation under a light microscope.

Evaluation guide:
(i) Target concept: Microscopic identification of rabies via pathognomonic Negri bodies in HE/Negri-stained brain tissue.
Minimum mechanism: Rabies virus infection must have occurred in neural tissue; viral replication must have produced cytoplasmic Negri bodies; dual staining (HE for architecture + selective Negri stain for inclusions) must be applied and preserved; light microscopy must be used at appropriate magnification (implied 10×–40×) to resolve subcellular features.
(ii)Storyline: [Frame 0–2] Static wide shot: experimental table with centered glass slide bearing stained brain tissue section. [Frame 3–5] Camera holds fixed viewpoint; light microscope enters frame from top or remains static with eyepiece vertically aligned over slide center. [Frame 6–10] Visual focus remains on slide surface: HE contrast (blue-purple nuclei / pink cytoplasm) is continuously visible; ≥1 magenta-red, round/ovoid, sharply bordered intracytoplasmic Negri body appears and remains stably resolved; no occlusion, motion blur, or fading. Perspective is frontal, orthogonal, and unmoving; no cutaways, zooms, or secondary objects distracting from slide-microscope relationship.

Manual:
Experimental Procedure
1. Place a glass slide bearing rabies-infected brain tissue section on the experimental table.
2. Ensure the section is stained with Hematoxylin-Eosin (HE) and Negri body-specific stain.
3. Position a light microscope with eyepiece aligned vertically over the slide.
Observation of Experimental Phenomena
Under the microscope: neurons show blue-purple nuclei and pink cytoplasm; within neuronal cytoplasm, distinct magenta-red, round-to-ovoid, sharply demarcated Negri bodies are visible.
Summary of Experimental Principles
Rabies virus replicates in neurons and forms characteristic Negri bodies—eosinophilic cytoplasmic inclusion bodies detectable by specific staining. HE staining provides general histologic context; Negri staining confirms rabies diagnosis.

Prompt Grounding rubric:
level_1: No experimental table is shown; no glass slide or brain tissue section is visible; there is no light microscope in the scene; nothing indicates a microscopic-observation setup is being depicted.
level_2: An experimental table is visible and a slide or tissue sample is present, but it shows no staining at all (no color contrast suggesting HE or Negri stain); no light microscope appears, or none is oriented toward the slide.
level_3: An experimental table is visible with a glass slide placed on it; the slide bears a tissue section with some pink/blue coloration suggestive of HE staining; a light microscope is present in the scene, oriented toward the slide, but no distinct inclusion bodies are visible within the tissue.
level_4: All elements from score 3 are present AND the slide sits flat and centered on the table; the tissue section shows a clear blue-purple/pink HE color contrast; the microscope's eyepiece is aligned over the slide; the tissue additionally shows some reddish-magenta staining distinct from the surrounding HE colors, though individual inclusion bodies are not yet clearly resolved as discrete rounded shapes.
level_5: All elements from score 4 are present AND the slide is the only slide in the scene, centered under the microscope's eyepiece with no other tools or distractors visible; the tissue section shows both the blue-purple/pink HE coloration and one or more clearly resolved, distinctly colored (magenta-red) rounded inclusions within the tissue, with the viewpoint fixed and front-facing throughout.

Scientific and Causal Correctness rubric:
level_1: The tissue shows no neuronal architecture and no nuclear-cytoplasmic color differentiation; no reddish inclusions are visible anywhere; colors used are biologically implausible (e.g., green nuclei, blue inclusions).
level_2: The tissue does not resemble brain parenchyma (e.g., looks like muscle or skin); HE coloring is inverted (cytoplasm blue, nuclei pink) or entirely monochrome; any reddish inclusions present sit outside cells, inside nuclei, or are clearly the wrong shape for Negri bodies (e.g., long fragmented streaks rather than compact rounded blobs).
level_3: The tissue shows recognizable brain parenchyma with visible neurons; HE coloring shows a rough nuclear-cytoplasmic contrast (bluish-purple nuclei, pinkish cytoplasm) though the contrast may be faint or inconsistent; one or more magenta-red rounded inclusions are visible within neuron cytoplasm, but their borders look smudged or their shape is irregular rather than clearly round/ovoid.
level_4: All elements from score 3 are present AND the HE coloring is consistent throughout (nuclei reliably blue-purple, cytoplasm reliably pink); at least one magenta-red, distinctly round-to-ovoid inclusion with a clean border sits inside neuron cytoplasm; every visible inclusion is inside a neuron's cell body (never inside a nucleus, never between cells, never inside a blood vessel).
level_5: All elements from score 4 are present AND the tissue is clearly recognizable as brain tissue with identifiable neuron cell bodies; the HE coloring is crisp and consistent (deep blue-purple nuclei, pale-to-medium pink cytoplasm, pale blue-tinted background); at least one Negri body is fully resolved (compact, round-to-ovoid, homogeneously magenta-red, with a sharp border and a visible lighter halo separating it from the surrounding cytoplasm), with no stain bleeding into the background and no inclusion misplaced outside the neuron.

Spatiotemporal Consistency rubric:
level_1: The slide or microscope appears or disappears abruptly between frames; the microscope's position jumps; the lighting flickers violently or its color temperature changes mid-clip; the slide rotates or flips with no physical cause; the slide appears to break apart or float.
level_2: The slide is present but shifts sideways or tilts more than roughly 15 degrees without any visible cause; the microscope's objective appears to move up or down with no corresponding motion of a focus knob; the shadow cast by the microscope changes direction abruptly; the table edge or other background elements visibly warp between frames.
level_3: The slide stays flat and stationary on the table throughout; the microscope stays fixed in position and orientation; the lighting stays steady in brightness and direction; nothing disappears or reappears; there is no sudden jump in scale or viewing angle; the slide remains positioned under the microscope's objective with the eyepiece centered above it.
level_4: All elements from score 3 are present AND the slide's edges stay straight and parallel across the whole clip; the microscope's eyepiece stays vertically aligned over the slide's center; there is no visible shaking or wobble of the slide or microscope; no reflection or lens flare covers up the inclusion bodies or key parts of the scene.
level_5: All elements from score 4 are present AND neither the slide nor the microscope shows any motion blur; the inclusion bodies are visible only once the slide itself is fully in view (never appearing before the slide/scene is established); there is no visible flicker or brightness pulsing anywhere in the scene; the camera viewpoint stays completely fixed with no panning, tilting, or zooming, so the slide and microscope occupy the same screen region in every frame.