Domain: Engineering — Civil & Environmental Engineering
Target concept: Validation Experiment of Petroleum-Degrading Bacteria's Degradation Effect

Video generation prompt: Equal volumes of oily wastewater were added to two flasks, one inoculated with petroleum-degrading bacteria and the other not inoculated. After 7 days of cultivation, the solutions in Flask A and Flask B were observed.

Evaluation guide:
(i) Target concept: Microbial biodegradation of petroleum hydrocarbons in aqueous phase results in quantifiable optical changes (reduced absorbance/scattering) in oily wastewater.
Minimum mechanism: Bacterial metabolic activity must be assumed active and specific to aliphatic/aromatic hydrocarbons; no abiotic oxidation or evaporation dominates; liquid phase remains homogeneous with no phase separation or biomass sedimentation obscuring optical assessment.
(ii)Storyline: Frame opens on static, frontal view of two identical flasks side-by-side on lab bench; both contain equal volumes of opaque yellow-brown liquid; a clear inoculation action (e.g., pipette tip contacting Flask A only) occurs before incubation cue (e.g., timer overlay or calendar flip); final frame shows both flasks unchanged in position, but Flask A liquid is visibly lighter and clearer than Flask B; viewpoint remains fixed, lighting constant, and no intervening actions; visibility constrained to direct frontal observation of liquid column only, excluding caps, lids, or air space above meniscus.

Manual:
Experimental Procedure
1. Place two identical clean flasks side-by-side.
2. Add equal volumes of identical oily wastewater to both.
3. Inoculate ONLY Flask A with petroleum-degrading bacteria; Flask B remains uninoculated.
4. Incubate both flasks undisturbed for 7 days.
5. Observe final liquid appearance in both flasks.
Observation of Experimental Phenomena
Flask A (inoculated) shows lighter-colored, more transparent liquid. Flask B (control) remains dark yellow-brown and opaque.
Summary of Experimental Principles
Petroleum-degrading bacteria metabolize hydrocarbons in oil, reducing pigment concentration and suspended organics → measurable decrease in color intensity and increase in solution transparency.

Prompt Grounding rubric:
level_1: No flasks are shown; no oily wastewater liquid is visible in any container; no bacterial inoculation step or comparison between an inoculated and non-inoculated flask is depicted; none of the prompt's objects (two flasks, oily wastewater, an inoculation action) appear.
level_2: Only one flask is shown, or two flasks appear but hold visibly different volumes of liquid; there is no visible action distinguishing an inoculated flask from a control flask, such as a pipette or dropper adding culture; the two flasks are never shown together side by side.
level_3: Two flasks of the same size and shape sit side by side, each holding an equal-looking volume of opaque yellow-brown oily wastewater; one flask is visually marked or shown being inoculated (for example a pipette adds liquid to it, or it carries a distinguishing label), while the other receives no such addition; both flasks and the inoculation distinction are present, though the flasks are not perfectly still throughout the shot.
level_4: Beyond what a 3 requires, both flasks stay in the same fixed spots on the bench for the entire video with no drift or repositioning; both liquid surfaces stay level and undisturbed, with no pouring, splashing, or stirring shown during observation; the marker or action identifying which flask was inoculated stays clear and unambiguous throughout.
level_5: Both flasks are identical in glass shape, size, and material and are lit the same way with matching shadows against the same background; the inoculation marker or action stays attached to the correct flask only, with no stray hand, tool, or mislabeled tag appearing in any frame.

Scientific and Causal Correctness rubric:
level_1: Both flasks look identical in color and clarity, or both liquids appear fully clear, or the inoculated flask ends up darker and more turbid than the uninoculated control. Any of these contradicts the expected effect of petroleum-degrading bacteria breaking down the oil.
level_2: The two flasks end up looking different, but not in a way tied to biodegradation: for example the inoculated flask is pale while the control looks equally pale, or the difference between the two looks arbitrary rather than linked to which flask received bacteria; the video gives no clear reason why one flask changed and the other did not.
level_3: The inoculated flask (Flask A) ends up visibly lighter and less turbid (pale yellow or straw-colored) than the uninoculated control (Flask B), which stays dark yellow-brown and opaque; this matches the expected effect of petroleum-degrading bacteria consuming hydrocarbons and reducing the oil-derived color and turbidity.
level_4: Beyond what a 3 requires, the inoculated flask is noticeably more see-through, for example its far wall or bottom becomes visible through the liquid, while the control stays uniformly hazy with no view through it; the difference in appearance reads as a genuine property of the liquids rather than an added visual effect.
level_5: Beyond what a 4 requires, the color shift in the inoculated flask is a straightforward lightening and clearing of the same yellow-brown hue, with no odd color cast such as green, blue, or red appearing in either flask; the change in clarity looks even across the whole liquid column in Flask A rather than patchy or layered.

Spatiotemporal Consistency rubric:
level_1: One or both flasks are missing from a frame and turn up again later in a different position with no incubation-passage cue (timer overlay, calendar flip, or fade) explaining the gap; the liquid level in a flask changes abruptly with no visible cause; the room lighting jumps between clearly different states from one frame to the next outside of a single marked incubation-passage transition; a flask's shape visibly warps or bends; or the liquid's meniscus flips orientation (e.g., switching from concave to convex) for no physical reason.
level_2: The two flasks shift position relative to each other during the video, for example one drifts sideways or the framing zooms in and out, other than a difference explained by the one marked incubation-passage transition; the curve of a liquid surface looks different from frame to frame without cause; shadows on the flasks appear, disappear, or swing direction without any change in lighting and without an accompanying incubation-passage cue; parts of the background flicker or break up.
level_3: Both flasks stay in the same side-by-side arrangement in the frames immediately before and immediately after the one permitted incubation-passage transition (a timer overlay, calendar flip, or fade) marking the 7-day gap between inoculation and observation; neither liquid surface shows ripples, waves, or motion blur; the lighting and the shadows cast by both flasks stay steady in direction and brightness within each of the two segments on either side of that transition; neither flask's glass or liquid boundary bends or deforms; there are no abrupt jumps or missing moments in the footage beyond that single marked transition.
level_4: Beyond what a 3 requires, any camera movement, such as a slow push-in, is smooth and keeps the two flasks in a believable spatial relationship to each other within each segment; if the camera is static, the flasks show no visible shake or drift; reflections on the glass move consistently with the viewpoint rather than floating independently; nothing, such as a hand, enters and then disappears mid-motion without a visible reason; the one incubation-passage transition is the only cut or jump in the entire video.
level_5: Beyond what a 4 requires, both liquid surfaces stay flat and level in every frame where visible, confirming neither flask was disturbed after cultivation; the video shows no visible flicker, streaking, or ghosting in the liquid or glass; the brightness and color of the scene stay steady from frame to frame within each segment with no pulsing or flashing.