Domain: Natural Science — Ecology & Evolutionary Biology
Target concept: Male Rapid Blue Flash vs. Female Slow Blue Flash in the European Firefly Squid

Video generation prompt: Display two squid models or animations in the dark. First activate the blue flash of the male model, then activate the blue flash of the female model, and focus the image on the duration and rhythm of each flash.

Evaluation guide:
(i) Target concept: Sexually dimorphic bioluminescent signaling in Watasenia scintillans, where flash kinetics (rapid vs. slow) serve as species- and sex-specific cues.
Minimum mechanism: Bioluminescence must be self-generated (not projected light), localized to squid anatomy (photophores), and temporally decoupled between individuals: no shared circuitry or external trigger synchrony.
(ii)Storyline: [Phase 1] Darkness establishes baseline; two static squid models appear simultaneously and remain fully visible. [Phase 2] Male model alone emits blue light in rapid pulse: onset and offset are crisp, duration is subjectively short. [Phase 3] Male flash fully terminates (complete darkness returns). [Phase 4] Female model alone emits blue light in slow pulse: onset and offset are crisp, duration is subjectively longer than male"s. [Phase 5] Both models remain continuously visible, centered, and unoccluded; camera perspective is fixed; no lighting, scaling, or positional change occurs at any point.

Manual:
Experimental Procedure
1. Place two anatomically accurate European Firefly Squid models side-by-side in complete darkness.
2. Illuminate only the male model with a rapid blue flash (discrete, short-duration pulse).
3. After male flash ends, illuminate only the female model with a slow blue flash (discrete, longer-duration pulse).
Observation of Experimental Phenomena
Male squid emits a brief, sharp blue pulse; female squid emits a prolonged, sustained blue pulse — both pulses visually distinct in duration and rhythm, observable side-by-side in fixed frame.
Summary of Experimental Principles
Male European Firefly Squid (Watasenia scintillans) produce rapid bioluminescent flashes for courtship signaling; females produce slower, more sustained flashes — a sexually dimorphic communication strategy under natural selection.

Prompt Grounding rubric:
level_1: No squid models appear; or only one squid model is ever visible; or no blue flash occurs at all; or the flash happens without any squid model on screen; or the scene is not dark when the models are shown.
level_2: Two squid models appear together in the dark, but the male's flash and the female's flash happen at the same time, or only one model ever flashes, or the flash color is clearly not blue (e.g., green, white, purple), or a flash occurs before the models have settled into view.
level_3: Two squid models sit visibly in the dark, settle into view before either one flashes, and flash in the correct order (the male model's blue flash first, then the female model's blue flash), but the two flashes look interchangeable, with no visible difference in how long each one lasts or how it turns on and off.
level_4: Everything needed for a 3 holds, and the male's flash and the female's flash are now clearly different from each other in duration (one plainly reads as a quick flash, the other as a longer flash), but one shortcoming remains, such as the camera cutting, panning, or zooming between the two flashes, the color tone shifting between them, or one squid model briefly leaving the frame.
level_5: Everything needed for a 4 holds with no remaining shortcoming: both squid models stay fully visible, in the same spots, under one continuous, unmoving camera view for the whole clip, and the male's flash is clearly shorter than the female's flash with nothing else about the framing, color, or visibility undercutting the comparison.

Scientific and Causal Correctness rubric:
level_1: The flashes are not blue, or the female's flash happens before or at the same moment as the male's, or both squid models flash with identical duration and rhythm, or the flashing subject isn't recognizable as this species of squid.
level_2: The blue color, squid identity, and male-then-female order are all correct, but the rhythm assignment contradicts the biology: for instance the male's flash is the longer one and the female's is the shorter one, or a flash fades in and out gradually instead of switching on and off crisply.
level_3: The male's blue flash reads as a short, sharply-bounded pulse and the female's blue flash reads as a longer, sharply-bounded pulse, matching the expected male-short/female-long pattern, but one of the two flashes doesn't quite have a clean on/off edge (e.g., a soft fade at the start or end).
level_4: Both flashes now have a crisp on/off edge with no fade, matching the correct short-male/long-female pattern, but one of the two shows an unrelated glitch (a flicker, an uneven brightness across the squid's body, or a stray brightness spike) that has nothing to do with the rhythm difference itself.
level_5: The male's flash is a clean, short, sharply-bounded pulse and the female's flash is a clean, longer, sharply-bounded pulse; both light the squid's body evenly with no flicker, fade, or stray brightness spike on either one.

Spatiotemporal Consistency rubric:
level_1: The squid models jump to a different spot between moments, or one vanishes and reappears, or a model's size or facing changes suddenly, or the dark background suddenly brightens as if a room light had switched on.
level_2: The models stay put, but one squid's body visibly stretches, twists, or breaks apart unnaturally, or the whole scene (not just the flashing squid) brightens when a flash begins, or a shadow appears to fall on the wrong side of the flashing squid relative to where its own light is coming from.
level_3: Both squid models hold their positions and shapes the whole time, the background stays dark throughout, and each flash lights up only the flashing squid and nothing behind it, but the gap between the male's flash ending and the female's flash beginning shows a visible stutter, blur, or brief drop-out.
level_4: The gap between the two flashes is smooth with no stutter, blur, or drop-out, and both squid models stay at the same scale and pose side by side the entire time, but one small blemish remains, such as a single brief flicker in overall brightness that doesn't affect which flash is which.
level_5: Both squid models stay perfectly still, at the same scale and pose, side by side, the background stays uniformly dark, and each flash appears only on its own squid at the intended moment, with a smooth, uninterrupted transition from the male's flash to the female's.