Domain: Engineering — Agricultural Engineering
Target concept: Soybean seeds were separately placed under conditions of 25°C and 4°C, verifying that seeds germinate rapidly at 25°C, while showing no significant changes at 4°C.

Video generation prompt: Close-up time-lapse photography demonstration. Two labeled petri dishes are lined with moist filter paper, and an equal number of soybean seeds are placed in each dish. One petri dish is placed in a 25°C incubator, while the other is placed in a 4°C refrigerator. Time-lapse photography is conducted continuously until the seeds germinate.

Evaluation guide:
(i) Target concept: Temperature-dependent seed germination via enzymatic activation and water uptake.
Minimum mechanism: Imbibition must occur in both dishes (moist paper present), but only at 25°C does sufficient kinetic energy enable enzyme-mediated cell wall loosening and radicle protrusion; at 4°C, membranes remain rigid and hydrolytic enzymes are inactive.
(ii)Storyline: Frame 0: Two labeled dishes with dry-appearing seeds on moist paper. → Swelling appears synchronously across all seeds in 25°C dish (no swelling in 4°C dish). → Radicles emerge from hilum of swollen 25°C seeds (none in 4°C dish). → All changes occur within fixed viewpoint, side-by-side spatial layout, and continuous forward time progression. Visibility constraint: Hilum, radicle tip, label text, and filter paper moisture gradient must remain fully discernible at all times.

Manual:
Experimental Procedure
1. Place two identical petri dishes side-by-side in fixed frame.
2. Line each dish with moist filter paper.
3. Place 5 soybean seeds evenly spaced on filter paper in each dish.
4. Label left dish "25°C", right dish "4°C".
5. Position dishes in respective environments (implied by labels) and begin time-lapse.
Observation of Experimental Phenomena
- Seeds in 25°C dish swell uniformly, then white radicles emerge from hilum.
- Seeds in 4°C dish show no swelling, no radicle emergence, no color/texture change.
Summary of Experimental Principles
Soybean seed germination requires adequate temperature; 25°C permits metabolic activation and imbibition-driven growth, while 4°C arrests cellular activity and prevents germination.

Prompt Grounding rubric:
level_1: None of the setup elements from the prompt are shown: no petri dishes, no soybean seeds, no 25°C/4°C labeling, no side-by-side dish layout, and no moist filter paper in either dish. The germination-comparison setup is not established at all.
level_2: At least one dish with a few visible seeds appears, but something essential is missing: only one dish is shown rather than two, neither dish is labeled 25°C or 4°C, filter paper is absent from both dishes, or the two dishes are not placed next to each other (e.g., stacked, overlapping, or shown in separate shots).
level_3: Two petri dishes, each holding roughly the same number of soybean seeds (at least three per dish) on visibly moist filter paper, sit next to each other in the same shot; one dish is clearly labeled 25°C and the other 4°C; and there is some sense of time passing (visible seed swelling or radicle growth) rather than a single static image.
level_4: All of 3, plus the labels correctly match their own dish (not swapped), the seeds are placed and oriented similarly within each dish, the filter paper fully covers the bottom of both dishes without gaps or curling, and the camera keeps both dishes in frame throughout without cropping or repositioning.
level_5: All of 4, plus the two dishes look like a matched pair (same size, shape, and plastic appearance), and the moist sheen on the filter paper looks similar in both dishes at the start, so that any difference a viewer later sees between the dishes reflects the seeds' response rather than the containers looking different to begin with.

Scientific and Causal Correctness rubric:
level_1: No biological change is visible in either dish: seeds in the 25°C and 4°C dishes look identical throughout, with no swelling and no radicle in either. The clip gives no evidence that temperature affects germination.
level_2: Swelling appears in only one dish, but the causal story is wrong: no radicle ever appears in either dish, a radicle appears in the 4°C dish, a radicle appears before any swelling in the 25°C dish, or the seeds discolor or brown before any radicle forms.
level_3: The 25°C dish shows visible seed swelling followed by white radicles emerging from the seeds; the 4°C dish shows no swelling and no radicle at any point; neither dish shows decay, mold, or discoloration.
level_4: All of 3, plus swelling in the 25°C dish always precedes radicle emergence for each seed that germinates (no radicle appears without prior swelling), and no seed in the 4°C dish shows even slight softening or a glossier, moister look.
level_5: All of 4, plus essentially all seeds in the 25°C dish follow the same swell-then-radicle sequence at a similar pace, the radicles are pale and emerge from the seed's hilum region, and the 4°C seeds look the same at the end of the clip as they did at the very start.

Spatiotemporal Consistency rubric:
level_1: Severe spatiotemporal rupture: a dish's position, its seeds, or its label are visibly different from one frame to the next with no pour, hand, or growth process shown causing the change; a seed goes from dry to fully sprouted between consecutive frames with no intermediate swelling shown; a dish's circular rim visibly warps or stretches out of shape, or the lighting swings abruptly between full shadow and full highlight with no shown cause; the camera viewpoint suddenly jumps, pans, or rotates instead of holding steady; or the two dishes trade places (25°C dish and 4°C dish swap sides) partway through the clip.
level_2: Several moderate breaks appear: a dish's lid lifts and resettles for no shown reason, the filter paper curls at one edge then flattens again without cause, a seed's swelling appears to reverse and then reappear, or a label is illegible or blocked from view for an extended stretch of the clip.
level_3: The two dishes and their 25°C/4°C labels stay in the same relative places throughout, each label always paired with the same dish; dish and seed outlines stay sharp, without dishes deforming or rotating unexpectedly; and neither label nor any seed is hidden from view for an extended stretch.
level_4: All of 3, plus the swelling-then-radicle progression in the 25°C dish advances smoothly from frame to frame rather than jumping straight from a dry seed to a long radicle, and no seed's stage reverses: a radicle never shrinks back into the seed.
level_5: All of 4, plus every visible change in the 25°C dish, such as seed swelling or radicle lengthening, plays out as one smooth, gradually progressing sequence without stutter or sudden jumps, while the seeds in the 4°C dish stay visually unchanged and stable in position for the entire clip.