Domain: Natural Science — Biochemistry & Molecular Biology
Target concept: ATP-dependent bioluminescent reaction (luciferase-catalyzed reaction, the core technology for ATP detection)

Video generation prompt: Add a small volume of ATP solution to a transparent vial containing luciferase and luciferin, mix thoroughly, and place in a dark environment.

Evaluation guide:
(i) Target concept: ATP-dependent bioluminescence requires enzymatic activation (luciferase), substrate (luciferin), and energy donor (ATP) to produce visible light; minimum mechanism: luciferase must be pre-complexed with luciferin, ATP binding induces conformational change enabling luciferin oxidation, and photon emission occurs only upon successful catalytic turnover in aqueous solution under oxygenated conditions.
(ii)Storyline: [Phase 1] Static shot: transparent vial centered, filled with clear-to-pale-yellow luciferase-luciferin solution, visible meniscus, no light. [Phase 2] ATP solution enters frame from top/right, travels along unbroken path, contacts liquid surface, and merges. [Phase 3] Vial undergoes coherent mixing motion (inversion/swirl) with visible internal fluid displacement; no spillage, no cap disturbance. [Phase 4] Frame cuts to or transitions into full darkness (black background, zero ambient cues); vial remains fixed. [Phase 5] Yellow-green light emerges solely from liquid volume, intensifies smoothly, conforms to meniscus shape, and decays without flicker or spread — all while vial stays motionless and fully visible.

Manual:
Experimental Procedure
1. Place a transparent vial containing pre-mixed luciferase and luciferin solution on a stable surface.
2. Add a small volume of ATP solution into the vial using a precise delivery tool.
3. Mix thoroughly by gentle inversion or swirling.
4. Immediately place the vial in complete darkness.
Observation of Experimental Phenomena
A transient yellow-green bioluminescent glow appears exclusively within the liquid volume of the vial after mixing, localized to the solution, and fades within seconds in darkness.
Summary of Experimental Principles
ATP binds luciferase, enabling oxidation of luciferin; this exergonic reaction emits photons at ~560 nm (yellow-green), serving as a quantitative, real-time indicator of ATP concentration.

Prompt Grounding rubric:
level_1: No transparent vial visible; no luciferase-luciferin mixture present; no ATP solution added; no mixing action shown; no dark environment established.
level_2: Transparent vial is present and contains a liquid, but the liquid is not pre-labeled or visually distinguishable as luciferase-luciferin mixture; ATP solution is shown but not added to the vial; mixing action is absent; dark environment is not established.
level_3: Transparent vial is present and contains a liquid (pre-mixed luciferase-luciferin); a small-volume ATP solution is visibly added into the vial; mixing action (e.g., swirling, inverting, or pipette agitation) is shown; dark environment is established before or during observation.
level_4: All elements of score 3 are present AND the ATP addition occurs directly into the vial (not beside/above without contact); mixing occurs while vial remains upright or inverted but fully contained within frame; dark environment is maintained continuously from mixing through observation phase.
level_5: All elements of score 4 are present AND the vial is clearly transparent (no opaque coating, label obscuration, or tint); the ATP solution is delivered via a fine-tip pipette or dropper showing discrete small-volume delivery; mixing is unambiguous (liquid motion visible inside vial, no static freeze); dark environment is absolute (no ambient light leakage, no visible room features, black background or fully shadowed enclosure).

Scientific and Causal Correctness rubric:
level_1: No bioluminescent emission observed; no color change; no light emission correlated with ATP addition; emitted light is white, blue, or green instead of yellow-green; light appears before ATP addition or persists after prolonged stillness.
level_2: Light emission occurs but is spatially disconnected from the vial (e.g., light source outside vial, glow on hand, or background illumination); emission color is incorrect (e.g., red, purple, cyan, or multi-color); light appears without prior ATP addition or mixing.
level_3: Yellow-green bioluminescent light appears exclusively within the vial interior after mixing; light onset follows ATP addition and mixing; no light before ATP addition; light is localized to liquid volume, not container walls or surroundings.
level_4: All elements of score 3 are present AND light intensity increases visibly during/just after mixing and stabilizes; light remains confined to liquid phase (no glow from air space above liquid or vial cap); no competing light sources (e.g., reflections, LED glints, or screen glow) are visible.
level_5: All elements of score 4 are present AND light appears only after mixing completes (no emission during addition-only phase); light distribution matches liquid shape and meniscus; no flicker, pulsation, or artificial strobing; emission ceases within observable timeframe after vial is left still in darkness (i.e., transient, not persistent).

Spatiotemporal Consistency rubric:
level_1: Vial teleports between frames; sudden appearance/disappearance of ATP solution or light; abrupt lighting shift mid-sequence; container deforms or changes transparency discontinuously; mixing motion jumps or reverses without physical continuity.
level_2: Vial remains in frame but shifts position >1/3 of frame width between key phases; ATP solution appears without visible delivery path; light emerges from non-liquid regions (e.g., cap, label, or empty air space) with no transition; mixing motion is jerky or interpolated (no fluid motion cues).
level_3: Vial stays fixed in frame throughout; ATP solution travels along continuous visible path into vial; mixing shows coherent fluid motion (e.g., swirl, rotation, or oscillation) inside vial; light emerges from liquid volume with smooth onset (no flash-cut); lighting remains consistent in darkness.
level_4: All elements of score 3 are present AND vial maintains identical orientation and position across all frames; ATP delivery path is fully visible from source to vial entry point; mixing motion is physically plausible (no floating droplets, no gravity-defying suspension); light onset begins at liquid surface/meniscus and propagates downward consistently.
level_5: All elements of score 4 are present AND no frame-level artifacts: no cut edits, no speed ramping, no morphing; vial surface reflects zero ambient light (consistent matte/black background); liquid meniscus remains stable during mixing (no splashing beyond internal flow); light emission exhibits natural falloff (brightest at center, dimmer at edges) matching liquid geometry.