Domain: Natural Science — Ecology & Evolutionary Biology
Target concept: Heat-tolerant seedlings versus heat-sensitive seedlings under short-term heatwave conditions

Video generation prompt: Heat-tolerant seedlings were planted in red pots and heat-sensitive seedlings in blue pots; both trays were placed side by side in the growth chamber during the heatwave, and the seedlings remained there even after the heating was turned off.

Evaluation guide:
(i) Target concept: Differential turgor loss under acute heat stress as a functional marker of heat tolerance in seedlings.
Minimum mechanism: Heat-sensitive seedlings experience faster stomatal closure failure and membrane leakage under elevated temperature, leading to rapid water loss and loss of leaf/stem rigidity; heat-tolerant seedlings sustain hydraulic conductivity and osmotic adjustment, preserving turgor and posture.
(ii)Storyline: Frame opens on tray containing red and blue pots side by side; heat-tolerant seedlings are planted in red pots, heat-sensitive in blue pots, all upright pre-heat; heatwave begins (implied by chamber context, no visual heater needed); after sufficient duration, heat-sensitive seedlings visibly wilt while heat-tolerant remain upright; final frame holds both states simultaneously in fixed side-by-side composition; viewpoint remains static, eye-level to tray, ensuring unobstructed comparison; no human intervention, labels, or time indicators shown.

Manual:
Experimental Procedure
1. Place heat-tolerant seedlings in red pots and heat-sensitive seedlings in blue pots.
2. Arrange all pots side by side in trays inside a growth chamber.
3. Initiate heatwave protocol; maintain throughout exposure and continue observation after heating stops.
Observation of Experimental Phenomena
Heat-tolerant seedlings (red pots) remain upright and turgid; heat-sensitive seedlings (blue pots) exhibit visible wilting (leaves and stems drooping downward) — both states coexist side by side.
Summary of Experimental Principles
Short-term heatwave induces differential physiological stress: heat-tolerant seedlings maintain cellular water balance and structural integrity; heat-sensitive seedlings lose turgor pressure rapidly, causing reversible wilting — a key ecophysiological trait distinguishing thermal resilience.

Prompt Grounding rubric:
level_1: No seedlings, pots, trays, or growth-chamber setting are shown; or the scene is unrelated (e.g., an outdoor field, an unrelated lab bench, or an animated diagram).
level_2: At least one pot color (red or blue) and one seedling are visible, but trays, growth-chamber context, or the side-by-side arrangement are missing; seedlings are not clearly planted in pots; or only one pot color appears with no color contrast.
level_3: Trays holding both red pots and blue pots are visible; seedlings are planted in both pot colors; the background reads as a growth chamber (plain walls, even controlled lighting); all pots and seedlings sit side by side within a single continuous shot.
level_4: All elements from 3-score are present, and red pots hold one seedling type and blue pots hold the other, matching the red = heat-tolerant / blue = heat-sensitive assignment stated in the setup; the two pot groups sit in the same tray or in adjacent trays with no gap or discontinuity between them; nothing extraneous (labels, thermometers, hands) blocks the view of the setup.
level_5: All elements from 4-score are present, and only red and blue pot colors appear (no third color); every pot sits flat and level on the tray surface with no visible tilting or perspective distortion; the camera holds a single frontal or slightly angled framing that keeps every pot and seedling base in view without cropping, for the entire clip.

Scientific and Causal Correctness rubric:
level_1: There is no biologically plausible stress response: e.g., all seedlings look identical and equally upright, or all are equally collapsed regardless of pot color; or the response is reversed, with red-pot (heat-tolerant) seedlings wilting while blue-pot (heat-sensitive) seedlings stay upright.
level_2: A change in seedling posture appears but doesn't track with heat tolerance: e.g., both pot colors wilt identically, or only one pot color changes while paired with the wrong color assignment, or wilting appears before the heatwave phase is implied to begin.
level_3: Seedlings in red pots (heat-tolerant) stay upright and firm; seedlings in blue pots (heat-sensitive) show visible wilting: leaves drooping, stems bending toward the tray; both states appear together in the same shot with no mixing across pot colors.
level_4: All elements from 3-score are present, and wilting appears only in blue-pot seedlings (no red-pot seedling wilts, no blue-pot seedling stays fully upright); the red-pot seedlings' upright posture holds steady with no intermittent drooping; the blue-pot wilting shows as drooping leaves and bending stems rather than browning or dried-out tissue, which would suggest damage beyond a short-term stress response.
level_5: All elements from 4-score are present, and the contrast is unambiguous: upright (red-pot) seedlings hold their leaves level or angled upward, while wilted (blue-pot) seedlings have at least two leaves clearly sagging below horizontal with stems curving toward the tray; no seedling shows a mixed posture (part up, part down); the difference reads clearly under the shot's lighting without motion blur obscuring it.

Spatiotemporal Consistency rubric:
level_1: Pots jump to new positions between frames; seedlings appear or disappear without any growth continuity; the lighting shifts abruptly (e.g., daylight to darkness within a second); the tray surface cracks, warps, or seedlings appear to float above it.
level_2: Pot positions shift sideways between frames without any visible motion connecting the two positions; seedlings flicker in or out of view; the tray's edge disappears and reappears; the lighting's brightness or direction changes inconsistently from frame to frame; a shadow points a different way with no matching change in the light source.
level_3: The red and blue pots stay in the same positions relative to each other throughout the clip; seedlings stay rooted in their own pots with no jumping; the tray surface stays flat and continuous; the lighting keeps a steady direction and brightness across the clip, with shadows landing consistently under that lighting.
level_4: All elements from 3-score are present, and nothing pops suddenly into view (no sudden dust, condensation, or reflections appearing from nowhere); the footage shows no frozen or repeated frames that read as stutter; the camera holds a single fixed framing with no zoom, pan, or rotation at any point.
level_5: All elements from 4-score are present, and the pot rims, tray corners, and seedling edges look crisp and stable with no shimmering or flickering texture; the overall brightness holds steady with no visible flashes or dips; if any subtle leaf tremor is visible, it moves smoothly and naturally rather than sliding, snapping, or jittering unnaturally.