Domain: Natural Science — Cell & Developmental Biology
Target concept: Fluorescently labeled glucocorticoid receptors translocate from the cytoplasm to the nucleus.

Video generation prompt: Live-cell fluorescence microscopy imaging of cultured mammalian cells expressing a green fluorescent glucocorticoid receptor; glucocorticoid hormone was added only to the left well during imaging.

Evaluation guide:
(i) Target concept: Ligand-induced nuclear translocation of steroid hormone receptors in living cells.
Minimum mechanism: Glucocorticoid hormone must bind cytosolic glucocorticoid receptor → induces conformational change → exposes nuclear localization signal → enables active transport through nuclear pore complex → accumulation in nucleoplasm. This requires intact cellular machinery (intact membranes, functional cytoskeleton, functional nuclear pores) and cannot occur without ligand addition.
(ii)Storyline: Frame 0–2: Static two-well dish view; green fluorescence uniformly diffuse in cytoplasm of cells in both wells; no pipette present. Frame 3–4: Pipette tip enters field of view and contacts liquid surface *only* in left well; solution delivery is visible as subtle meniscus disturbance. Frame 5–10: In left well only, green signal intensifies *within* stable nuclear boundaries, progressively exceeding cytoplasmic brightness; nuclear shape remains consistent and contiguous; right well shows zero change in signal distribution or intensity. Perspective: Fixed wide-field or confocal-style view; entire left and right wells remain fully in frame; nuclear regions must be optically resolvable (not pixelated blobs); no zoom, pan, or rotation.

Manual:
Experimental Procedure
1. Place two-well culture dish containing live mammalian cells expressing green fluorescent glucocorticoid receptor under microscope.
2. Record baseline fluorescence: green signal is diffuse in cytoplasm of cells in both wells.
3. Add glucocorticoid hormone solution exclusively to the left well using a fine pipette.
Observation of Experimental Phenomena
Green fluorescence remains diffuse/cytoplasmic in right well cells; in left well cells, green signal progressively accumulates inside morphologically identifiable nuclei — nuclear regions become distinctly brighter than surrounding cytoplasm.
Summary of Experimental Principles
Fluorescently labeled glucocorticoid receptors translocate from the cytoplasm to the nucleus upon hormone binding.

Prompt Grounding rubric:
level_1: No two-well culture dish is visible; no green fluorescent signal appears anywhere in the frame; no hormone-addition action (e.g., pipetting into a well) is depicted; and there is no visual indication of which side is 'left' versus 'right.'
level_2: A dish is shown but it is a single well or an unidentifiable multi-well arrangement (not clearly two side-by-side wells); green fluorescence is present but not clearly localized to cell-like structures in identifiable wells; the hormone-addition action is missing or ambiguous (e.g., the pipette tip never visibly touches the liquid, or no delivery motion is shown); or the left/right layout of the dish cannot be determined from the shot.
level_3: A two-well dish is clearly shown with two side-by-side wells; green fluorescence is visible in cells in both wells at the start of the video; but either no hormone-addition action is depicted at all, or the depicted addition is not confined to the left well (e.g., the pipette delivers to the right well, to both wells, or to the boundary between them).
level_4: A two-well dish is clearly shown with two side-by-side wells; green fluorescence is visible in cells in both wells at the start; the pipette tip is clearly shown contacting the liquid and delivering solution exclusively into the left well, with no contact or delivery into the right well; both wells remain identifiable and are not confused with one another; but the camera briefly zooms, pans, or shifts framing at some point, or one well is partially occluded (e.g., by the pipette or a reflection) for part of the video, so the setup is not shown with full, continuous visual clarity.
level_5: A two-well dish is clearly shown with two side-by-side wells, fully and continuously visible with fixed framing (no zoom, pan, or rotation) for the entire video; green fluorescence is visible in cells in both wells at the start; the pipette tip is clearly shown contacting the liquid and delivering solution exclusively into the left well, with no contact, splash, or delivery reaching the right well; neither well is ever occluded, and the left/right identity of each well remains unambiguous throughout: the setup and hormone-addition action are depicted with complete fidelity to the described experiment, independent of whatever fluorescence changes follow.

Scientific and Causal Correctness rubric:
level_1: Green fluorescence is absent, miscolored (e.g., red/yellow/blue), or appears in non-cellular locations (e.g., medium, dish walls); no nuclear translocation occurs anywhere; signal does not originate from mammalian cells.
level_2: Green fluorescence is present but static in all locations; nuclear translocation is absent in both wells; fluorescence appears in non-biological structures (e.g., bubbles, debris) or lacks cellular morphology; signal appears in nucleus before addition or without hormonal trigger.
level_3: Green fluorescence originates from cultured mammalian cells in both wells; initial signal is diffuse/cytoplasmic in both wells; no translocation occurs in right well; translocation occurs in left well but is discontinuous (e.g., abrupt jump, flicker, teleportation of signal), or nuclear accumulation is not visually distinguishable from cytoplasmic background.
level_4: Green fluorescence originates from cultured mammalian cells in both wells; initial signal is diffuse/cytoplasmic in both wells; no translocation occurs in right well (signal remains uniformly cytoplasmic); translocation occurs in left well: green signal progressively accumulates in nuclear regions (nuclear shape is discernible and signal inside nuclear boundary is brighter than surrounding cytoplasm), and this accumulation begins *after* glucocorticoid addition and proceeds continuously.
level_5: Green fluorescence originates from cultured mammalian cells in both wells; initial signal is diffuse/cytoplasmic in both wells; no translocation occurs in right well (signal remains uniformly cytoplasmic with no nuclear brightening); translocation occurs *only* in left well: green signal progressively and continuously accumulates *exclusively* within morphologically identifiable nuclear boundaries (nuclear signal intensity is clearly brighter than adjacent cytoplasm *and* brighter than nuclear signal in right well), and accumulation initiates within the same continuous visual sequence immediately following glucocorticoid addition action.

Spatiotemporal Consistency rubric:
level_1: Severe spatiotemporal violations: dish or cells teleport between frames; sudden appearance/disappearance of wells, cells, or fluorescence; abrupt light/shadow shifts unrelated to motion; fluorescence flickers on/off discontinuously; nuclear regions warp or dissolve mid-sequence.
level_2: Moderate violations: well boundaries shift or distort across frames; cell positions jitter unnaturally; fluorescence brightness jumps without gradual transition; pipette movement shows jerky motion or violates physical continuity (e.g., tip enters dish then reappears outside without exit path); nuclear regions lose structural coherence during putative translocation.
level_3: Minor violations: slight brightness fluctuation in background not affecting signal interpretation; minor jitter in dish position (<10% of dish width) that does not obscure well identity or left/right labeling; fluorescence intensity changes smoothly but nuclear boundary definition blurs momentarily without loss of nuclear shape recognition.
level_4: No teleportation, no structural breaks, no abrupt light/shadow shifts; dish remains fixed in frame with stable side-by-side well geometry; cells maintain consistent morphology and position; pipette motion is smooth and physically plausible; fluorescence intensity changes gradually and continuously; nuclear boundaries remain consistently recognizable throughout.
level_5: Zero spatiotemporal flaws: dish position and orientation are perfectly stable; well geometry is rigidly preserved; cell morphology and relative positions are invariant; pipette motion is smooth, continuous, and terminates with precise contact only in left well; fluorescence evolution is fully continuous (no frame drops, no flicker, no interpolation artifacts), and nuclear accumulation progresses with unbroken visual continuity and morphological fidelity.