Domain: Healthcare — Nutrition and Food Hygiene
Target concept: Borax Detection

Video generation prompt: A test tube containing 10 mL of food extract with borax was treated with curcumin-oxalic acid solution, then thoroughly mixed and heated in a 70°C water bath for 5 minutes.

Evaluation guide:
(i) Target concept: Chromogenic detection of borax via curcumin-oxalic acid complexation under thermal activation.
Minimum mechanism: Curcumin must be protonated by oxalic acid, then undergo boron chelation to form rosocyanine; this reaction is thermally accelerated and yields red-pink color exclusively in presence of borate ions.
(ii)Storyline: [Frame 0–2] Test tube holds pale yellow food extract → [Frame 3–4] Orange-yellow curcumin-oxalic acid solution is dispensed into tube → [Frame 5–6] Tube is swirled: liquid homogenizes to uniform orange-yellow → [Frame 7–8] Tube is lowered into water bath: liquid level stable, no spill → [Frame 9] After full heating duration, liquid transforms to uniform red-pink; all transitions occur from fixed frontal viewpoint with consistent framing; no off-screen actions; heating context must be visually anchored (water surface, steam, temperature label, or thermal haze).

Manual:
Experimental Procedure
1. Place 10 mL food extract into clean test tube.
2. Add curcumin-oxalic acid solution to tube.
3. Mix thoroughly by swirling.
4. Place tube in 70°C water bath for 5 minutes.
Observation of Experimental Phenomena
Food extract + curcumin-oxalic acid solution remains orange-yellow before heating; after 5 min heating, liquid turns uniform red-pink.
Summary of Experimental Principles
Borax in food extract reacts with curcumin under acidic (oxalic acid) and heated conditions to form a red-pink rosocyanine complex — a specific colorimetric detection method.

Prompt Grounding rubric:
level_1: None of the three core objects (test tube, food extract, curcumin-oxalic acid solution) appear in the shot; no addition, mixing, or heating action is shown at all.
level_2: At least one core object appears (test tube, food extract, or curcumin-oxalic acid solution), but none of the required actions (adding, mixing, heating) actually happen on screen, or the objects appear in a spatial arrangement that has no relation to a genuine benchtop setup.
level_3: The test tube holding the food extract, the curcumin-oxalic acid solution, and a water bath (or another clear sign of ~70°C heating, such as steam, a temperature label, or visibly warm water) are all shown; the curcumin-oxalic acid solution is added into the tube, the contents are mixed, and the tube is then placed into the heated water; these three actions happen in that order.
level_4: Everything described at the 3-point level holds, and in addition: the liquid inside the tube visually reads as roughly 10 mL relative to the tube's height and stays that way once the curcumin-oxalic acid solution is stirred in; the curcumin-oxalic acid solution is poured or dispensed in as a visibly separate stream rather than appearing pre-mixed; the mixing is shown as active swirling or inversion rather than a static cut; the heating is clearly a water bath (the tube surrounded by heated water) rather than a flame, oven, or dry heat source.
level_5: Everything described at the 4-point level holds, and in addition: the test tube stays in frame for the full sequence with no cut-away or repositioning of the camera; the liquid stays inside the tube during mixing with no spill or overflow; the water bath shows continuous gentle ripples or rising steam once the tube is submerged; the whole sequence (addition, mixing, submersion, and heating) plays out as one uninterrupted take with no jump cuts, time-lapse skip, or freeze-frame.

Scientific and Causal Correctness rubric:
level_1: The liquid in the tube shows no color change at all after mixing and heating, or it ends in a color other than red-pink (e.g., it stays orange-yellow, or turns white, blue, green, or brown); nothing about the final appearance of the liquid visually signals that borax has been detected.
level_2: A color change happens, but the wrong one (e.g., purple, black, or a fading rather than a deepening color); or a red-pink color shows up before the tube has been mixed, before it has been placed in the water bath, or before the curcumin-oxalic acid solution has even been added, breaking the required addition-then-mix-then-heat sequence.
level_3: The liquid turns red-pink only after both the mixing and the heating steps are complete; no red-pink tint appears at any earlier point in the sequence; before this, the food extract reads as pale yellow and the curcumin-oxalic acid solution as orange-yellow, never red-pink themselves; the red-pink color is a property of the liquid itself, not a film or precipitate floating on top.
level_4: Everything described at the 3-point level holds, and in addition: the red-pink color fills the liquid evenly with no streaking or layering; the contrast between the orange-yellow pre-heating liquid and the red-pink post-heating liquid is unambiguous; no other tint (a blue-ish or green-ish cast) competes with the red-pink; once it appears, the red-pink color holds steady rather than fading or darkening for the rest of the shot.
level_5: Everything described at the 4-point level holds, and in addition: the red-pink color only becomes clearly visible in roughly the last 10-15 seconds of the shown heating period rather than appearing earlier in the sequence; the hue reads as a red-pink (rosocyanine-like) shade rather than magenta or coral; the color looks even across the visible liquid without edge-darkening or a vignette-like fade toward the tube walls; the meniscus and inner tube wall don't show a separate patch of discoloration distinct from the bulk liquid.

Spatiotemporal Consistency rubric:
level_1: The liquid volume inside the tube jumps between frames with no pouring, spilling, or evaporation shown (e.g., it looks full, then half-full, then full again); the whole tube momentarily goes missing from a frame with no cut shown to explain it; the tube appears to pass through the solid rim of the water bath as it's lowered in; the tube visibly cracks or bends with no impact or heat-stress event shown; the tube appears to spin or rotate by more than roughly 90° with no hand or grip motion causing it; the camera cuts to a new angle mid-action.
level_2: The tube's liquid level looks inconsistent across the swirling motion (e.g., it appears to slosh out and then is shown full again the very next frame); the tube spins or rotates in the frame with no visible hand or grip motion causing it; the camera zooms or jumps between shots rather than moving smoothly; part of the tube is hidden behind an object that wasn't present moments earlier.
level_3: The liquid volume in the tube stays visually consistent from before mixing through the end of heating, aside from the deliberate addition of the curcumin-oxalic acid solution; the swirling looks like one continuous hand or wrist motion rather than a jump cut; the tube's descent into the water bath is a single continuous downward motion; at most one small, brief flaw appears, such as the tube base being partly hidden by the bath's rim for a moment as it goes in.
level_4: Everything described at the 3-point level holds, and in addition: the tube's tilt angle stays roughly steady (within about ±5°) through the mixing and heating portions except during the deliberate lowering motion itself; the liquid surface inside the tube stays a single continuous surface throughout swirling with no splashing outside the tube and no sudden foam; once the tube is submerged, the water bath's surface shows a steady, continuous ripple rather than going suddenly still or turning chaotic.
level_5: Everything described at the 4-point level holds, and in addition: the swirling and lowering motions look smooth from frame to frame with no sudden stutters or skips; the liquid meniscus inside the tube shifts gradually as the tube tilts rather than snapping to a new level; any steam or heat shimmer above the water bath drifts steadily upward rather than flickering on and off; once set into the bath, the tube's resting position stays fixed there for the rest of the heating shot with no unexplained drift.