Domain: Healthcare — Pathology
Target concept: Inflammation (chronic inflammatory cell infiltration)

Video generation prompt: A chronic inflammatory tissue section is placed on the experimental table, and cell infiltration is observed under the optical microscope.

Evaluation guide:
(i) Target concept: Visual identification of chronic inflammatory cell infiltration in a histological section via light microscopy.
Minimum mechanism: The tissue must be histologically processed and stained (e.g., H&E); the microscope must be optically functional at medium magnification; mononuclear cells must be morphologically distinguishable and spatially organized in a pattern consistent with chronic (not acute) inflammation.
(ii)Storyline: [Phase 1] Static wide shot: experimental table with unoccupied space → [Phase 2] Hand places stained tissue section onto center of table → [Phase 3] Microscope is positioned adjacent to section (same plane, fixed viewpoint) → [Phase 4] Camera cuts to or zooms into microscope eyepiece view, revealing a stable, in-focus histological field → [Phase 5] Within that field, dense clusters of mononuclear cells appear in interstitial spaces, morphologically distinct from epithelial or stromal elements, with no acute inflammatory features. Visibility constraint: Eyepiece view must be fully visible and dominant during Phase 5; no occlusion, no out-of-focus foreground, no simultaneous display of table and eyepiece unless using split-screen with strict spatial adjacency.

Manual:
Experimental Procedure
1. Place chronic inflammatory tissue section (H&E-stained) onto experimental table.
2. Position optical microscope beside the section.
3. Observe the tissue under microscope at medium magnification (10× objective).
Observation of Experimental Phenomena
A histological field showing dense interstitial infiltration by mononuclear cells (lymphocytes and plasma cells), blending gradually into adjacent normal tissue without a sharp border; no neutrophils or fibrin exudate present.
Summary of Experimental Principles
Chronic inflammation is characterized by prolonged immune response dominated by mononuclear cells — lymphocytes, plasma cells, and macrophages — infiltrating tissues, often associated with tissue destruction and fibrosis.

Prompt Grounding rubric:
level_1: No tissue section visible on experimental table; no optical microscope present; no observable placement action; no view showing microscope eyepiece or field of view.
level_2: Tissue section appears on table OR optical microscope appears in frame, but not both; placement action is absent or misattributed (e.g., hand places unrelated object); no microscope eyepiece view shown.
level_3: Chronic inflammatory tissue section is placed on experimental table AND optical microscope is present in same scene; placement action is visually completed (hand releases slide onto table); microscope eyepiece view is absent or shows blank/black/blurry field with no cellular detail.
level_4: All elements from score 3 are present, AND the eyepiece view shows a clearly in-focus histological field with visible cellular material (not blank, blurry, or a solid color) filling most of the frame, AND the view holds steady without repeatedly drifting in and out of focus.
level_5: All elements from score 4 are present, AND the eyepiece view is centered and fills the frame without dark vignetting or a cut-off field edge, AND the microscope, table, and slide remain visible together before the transition into the eyepiece view, with only the single storyline-prescribed cut or zoom used to move into the eyepiece view and no other cutaways or inserted close-ups anywhere else in the sequence.

Scientific and Causal Correctness rubric:
level_1: No biological tissue present; microscope shows non-biological objects (e.g., fabric, metal, text); cells appear as geometric shapes, uniform spheres, or cartoon-like forms; no plausible histological structure.
level_2: Tissue section is present but appears necrotic, hemorrhagic, or acutely inflamed (e.g., neutrophil-dominated exudate, pus, fibrin); microscope field shows only adipose, muscle, or epithelium without any infiltrating cells; coloration is absent or non-histological (e.g., uniform blue, neon green, grayscale without contrast).
level_3: Tissue section is stained (e.g., H&E) and shows baseline histological architecture; microscope field contains cells, but infiltration is ambiguous: cells are evenly distributed, sparse, or aligned along vessels without interstitial clustering; no discernible mononuclear predominance.
level_4: Microscope field shows mononuclear cells (lymphocytes/plasma cells) present AND clustered in interstitium AND adjacent to normal tissue structures (e.g., glands, ducts, collagen bundles); however, at least one of the following is missing: (a) the mononuclear cells clearly outnumbering other cell types in the infiltrated area, (b) absence of acute inflammatory cells (neutrophils), (c) presence of plasma cell morphology (eccentric nucleus, clock-face chromatin) or lymphocyte size uniformity.
level_5: Microscope field shows chronic inflammatory infiltration meeting all three criteria: (a) dense interstitial clusters of mononuclear cells that clearly outnumber any other cell type in the field, (b) no neutrophils, fibrin, or acute exudate anywhere in the field, (c) plasma cells (eccentric nucleus, deeply basophilic cytoplasm) and small lymphocytes (dense round nucleus, scant cytoplasm) are both individually identifiable within the clusters; the infiltrate may blend gradually into the surrounding tissue rather than having a sharp border, consistent with typical chronic inflammation.

Spatiotemporal Consistency rubric:
level_1: The scene cuts between unrelated locations (e.g., the lab bench suddenly becomes an outdoor scene); the microscope disappears and reappears; the tissue section's position or orientation changes between frames with no visible motion causing it; the whole scene switches between bright and pitch black with no visible cause.
level_2: The shot stays continuous, but with a major break: the tissue section visibly warps, tears, or turns blocky; the microscope's lens barrel rotates or tilts with no hand or knob shown causing it; the eyepiece view jumps between very different magnifications instantly with no zooming motion shown in between.
level_3: All objects stay in the same relative positions; nothing teleports or reshapes; the hand's motion placing the slide onto the table looks physically plausible; the eyepiece view enters the frame through a visible zoom, pan, or clean cut consistent with the storyline's transition into the eyepiece view, though the view may briefly go soft or freeze for a moment before settling.
level_4: All conditions from score 3 are satisfied, AND once the eyepiece view appears it stays in continuous focus for as long as it is shown (no unexplained blurring in and out); there is no sudden change in brightness or contrast within that view; the transition from the hand finishing the slide placement to the eyepiece view appearing happens as a single deliberate zoom, pan, or cut, not an unexplained or repeated jarring cut.
level_5: All conditions from score 4 are satisfied, AND every spatial relationship holds without drifting: the tissue section stays centered on the table for as long as it is visible, the microscope does not shift on the table, and the eyepiece view looks like a normal upright microscope view (not mirrored, rotated, or upside-down) with anything outside the focal plane softly blurred the way a real lens would render it; no part of the shot appears to stutter, double up, or bend out of shape.