Domain: Healthcare — Pharmacy
Target concept: Comparison of the Diffusion of Liposome-Encapsulated Dye and Free Dye in Water

Video generation prompt: Two identical transparent beakers are placed side by side, each containing still distilled water; at the same time, liposome-encapsulated blue dye is added to the left beaker, and free blue dye is added to the right beaker.

Evaluation guide:
(i) Target concept: Diffusion kinetics difference due to molecular confinement.
Minimum mechanism: Liposomes must remain structurally intact in water (no premature rupture), and free dye must be fully soluble and non-aggregating; water must be quiescent (no convection) to isolate diffusion-driven transport.
(ii)Storyline: [Frame 0–1] Static wide shot showing two identical beakers with clear, still water; [Frame 2–3] Dropper tips enter frame simultaneously from top, aligned vertically over left/right beaker centers; [Frame 4] Identical blue droplets detach and contact water surfaces, causing symmetric transient dimples; [Frame 5–9] Free dye cloud expands radially with smooth intensity gradient; liposome-encapsulated dye retains compact, high-saturation shape with sharp boundaries; [Frame 10] Contrast in spread extent is maximally visible: free dye occupies ≥3× the area of liposome-encapsulated dye. Perspective: Fixed frontal orthographic view, centered on symmetry axis, no occlusion, full beaker height visible.

Manual:
Experimental Procedure
1. Place two identical transparent beakers side-by-side on a flat surface, each filled with still distilled water.
2. Simultaneously add liposome-encapsulated blue dye to the left beaker and free blue dye to the right beaker.
Observation of Experimental Phenomena
Free blue dye rapidly diffuses outward from entry point, forming an expanding, radially graded blue cloud. Liposome-encapsulated blue dye remains tightly localized near entry point with minimal outward spread and uniform internal color density.
Summary of Experimental Principles
Liposome encapsulation impedes passive diffusion by acting as a physical barrier; free dye molecules diffuse freely via Brownian motion, resulting in faster and broader dispersion in aqueous medium.

Prompt Grounding rubric:
level_1: One of the two beakers is missing from the shot, or only one appears; or the beakers are not placed side by side; or neither beaker shows still distilled water; or blue dye is never added to either beaker; or dye is added to only one beaker while the other stays clear; or dye is added to the same beaker twice instead of one per beaker; or nothing in the video distinguishes which beaker holds the liposome-encapsulated dye and which holds the free dye.
level_2: Two transparent beakers with still liquid are shown side by side, and dye is added to both, but: dye reaches only one beaker while the other never receives any; or both beakers receive the same kind of dye (both liposome-encapsulated, or both free); or the two additions happen at clearly different times rather than together; or there is no visual or textual cue anywhere in the clip indicating which side is liposome-encapsulated and which is free.
level_3: Two identical transparent beakers sit side by side, each holding still liquid; blue dye is added to both at essentially the same moment; the left beaker is identifiable as receiving liposome-encapsulated dye and the right as receiving free dye, made clear by an on-screen label, a color-coded marking on one container, or a spoken/captioned statement naming which side is which within the first 3 seconds.
level_4: Everything required at the 3-point level holds, and in addition: the two beakers match each other in shape, size, and transparency; the liquid in both is clearly colorless and undisturbed before the dye touches it; and in both beakers the dye's introduction is shown as a distinct, visible action (a dropper tip entering frame and a drop detaching and falling) occurring at the same visual moment on each side.
level_5: Everything required at the 4-point level holds, and in addition: which beaker is liposome-encapsulated and which is free stays identifiable throughout the full 10-second clip through a persistent marker established at the moment of addition (such as a labeled dropper tip, a colored ring or tag on one beaker, or an on-screen caption that stays visible or reappears) rather than being stated once and then untraceable; and the left/right symmetry of the setup is reinforced by a fixed, centered camera framing in which both beakers appear at matching height and distance from the lens.

Scientific and Causal Correctness rubric:
level_1: No diffusion is visible in either beaker; or both beakers show the same spreading pattern and speed with no visible difference; or the dye color appears instantly throughout the liquid rather than spreading outward from where it entered; or the liposome-encapsulated dye shows literally zero movement (staying as an undisturbed single drop or pellet with no softening of its edges at any point) while the free dye spreads normally.
level_2: Diffusion appears in only one beaker while the other shows none at all; or both beakers diffuse at the same visible rate with no contrast between them; or the liposome-encapsulated dye spreads faster or wider than the free dye (reversed from expected); or the free dye reaches near-full dispersal within about 2 seconds while the liposome-encapsulated dye shows only the faintest softening at its edges: some minimal spread is visible, but it is far less than what a fully free dye would show.
level_3: Both beakers show visible diffusion: the free blue dye spreads outward continuously from where it entered, forming an expanding cloud; the liposome-encapsulated blue dye spreads noticeably more slowly, staying concentrated near its entry point with only modest outward growth; by the end of the clip, the difference in how far each dye has spread is clearly visible.
level_4: Everything required at the 3-point level holds, and in addition: the free dye's cloud is darkest near its center and fades gradually toward its edges rather than having a flat, uniform color throughout; the liposome-encapsulated dye keeps a comparatively sharp outer edge and even color inside that edge, without fading into the surrounding water; and the two dyes do not mix with each other or cross between beakers.
level_5: Everything required at the 4-point level holds, and in addition: the free dye cloud keeps expanding smoothly, without pausing, splitting into disconnected blobs, or suddenly jumping in size; the liposome-encapsulated dye mass stays intact: it doesn't dissolve away, burst, or shed loose particles into the surrounding water, and it shows no obvious internal swirling; and at every point after the dyes are added, the free dye's spread is visibly larger than the liposome-encapsulated dye's spread, with the gap between them never closing or reversing.

Spatiotemporal Consistency rubric:
level_1: A severe break in continuity occurs: the two beakers' positions or the spacing between them along the fixed left/right symmetry axis suddenly changes from one frame to the next with no camera movement shown; or the water level in either beaker jumps up or down abruptly with no pouring or spilling shown; or the lighting shifts abruptly, such as the scene suddenly turning bright or dark with no visible cause; or a beaker's outline warps into a different shape (e.g., its cylindrical wall bends or its base changes width); or the blue dye appears already inside the water with no dropper, pipette, or hand ever shown delivering it.
level_2: A moderate break occurs: the frame flickers or judders noticeably between frames; the water surface in one or both beakers looks perfectly flat with no meniscus curve, or stays completely undisturbed even as a dye drop lands on it; or the dye drop's motion skips: it is above the water in one frame and already submerged in the next with no drop shown crossing the surface.
level_3: Both beakers stay in the same fixed position and size throughout, viewed from a consistent frontal angle; each liquid surface shows a natural curved meniscus and a plausible reflection of the surroundings; the dye visibly enters through a dropper tip that dips into frame and touches the water surface in both beakers; neither beaker's wall or base shows tearing, clipping, or collapse; and the beakers' cast shadows on the surface beneath them keep the same length and direction on both the left and right side throughout the clip, without one side's shadow shifting while the other stays put.
level_4: Everything required at the 3-point level holds, and in addition: the dropper tip touches the water at the same relative height in both beakers; the small dimple the drop makes on impact looks similar in size and shape on both sides; the camera frame itself shows no drift or wobble over the clip; and anything visible behind the beakers (table edge, wall, other props) stays fixed with no unexplained shifting or refocusing.
level_5: Everything required at the 4-point level holds, and in addition: the motion throughout reads as smooth and continuous, with no stutter, dropped frames, or motion blur that looks mismatched to how fast things are moving; the small surface dimple from each drop's impact settles back to a flat surface smoothly in both beakers; and the drop's entry into the water looks physically coherent in both beakers: no drop hovering just above the surface, no drop appearing already underwater without breaking the surface, and no gap or hole left in the water where it entered.