Domain: Healthcare — Pathophysiology
Target concept: Acute Appendicitis

Video generation prompt: A paraffin section of the appendix from a patient with acute appendicitis was placed on a slide, stained with hematoxylin and eosin (H&E), and examined under a light microscope at increasing magnifications, from low to high power.

Evaluation guide:
(i) Target concept: Histopathologic diagnosis of acute appendicitis via H&E-stained paraffin section microscopy.
Minimum mechanism: The tissue must be human appendix, fixed in formalin, embedded in paraffin, sectioned at 4–5 μm, stained with hematoxylin (nuclear dye) and eosin (cytoplasmic dye), mounted under glass, and imaged under brightfield illumination with optical magnification, enabling visualization of neutrophil infiltration as the defining acute inflammatory feature.
(ii)Storyline: [Frame 0–3s] Static wide-field view: full slide in focus, showing rectangular glass slide with centered tissue section, faint coverslip reflections, and characteristic H&E color contrast; appendiceal wall layers (mucosa, muscle, serosa) are coarsely visible. [Frame 3–7s] Smooth, continuous optical zoom-in: field narrows radially while maintaining centering and focus; tissue texture sharpens, revealing individual cells. [Frame 7–10s] Stable high-magnification view: neutrophils appear as small, round, hyperchromatic, multi-lobed cells densely packed in lamina propria and penetrating muscularis; crypt abscesses visible as neutrophil-filled glandular lumens; no vascular congestion or necrosis dominates. Perspective is fixed frontal microscope view; no rotation, tilt, or oblique angle; all visibility constraints respect real optical limits (no sub-diffraction detail, no fluorescence, no 3D reconstruction).

Manual:
Experimental Procedure
1. Place H&E-stained paraffin section of human appendix on glass microscope slide.
2. Mount with coverslip using permanent mounting medium.
3. View under light microscope: first at low power (4x or 10x objective) to locate tissue; then switch to high power (40x objective) to examine cellular detail.
Observation of Experimental Phenomena
- Low power: layered tubular structure with central lumen, mucosal folds, and concentric muscle layers.
- High power: dense neutrophil infiltration in lamina propria and muscularis, crypt abscesses, and preserved epithelial integrity.
Summary of Experimental Principles
Acute appendicitis is characterized histologically by neutrophil-predominant transmural inflammation of the appendix wall, initiated by luminal obstruction and bacterial overgrowth, leading to mucosal ulceration and recruitment of acute inflammatory cells.

Prompt Grounding rubric:
level_1: No paraffin-embedded appendix tissue, no glass slide, and no H&E staining appear anywhere, and there is no sign of any change in magnification across the clip.
level_2: A tissue section sits on a glass slide, but it cannot be identified as appendix (no recognizable wall layers), the staining is not H&E-like (single-color or flat), and the clip shows no distinguishable difference between a wider and a narrower field of view.
level_3: A paraffin-embedded section on a standard glass slide shows genuine H&E coloring (blue-purple nuclei against pink-red cytoplasm), and the clip includes at least one clearly wider (low-power) view and one clearly narrower (high-power) view of what reads as the same specimen.
level_4: The tissue section stays centered in both the low-power and high-power views, and the coverslip's reflection or the mounting medium's sheen looks the same in both views, confirming both views are of the same mounted slide rather than two different specimens.
level_5: The low-power view shows the appendix's overall wall architecture (mucosa, muscularis, and serosa layers in roughly correct order from lumen outward), the high-power view resolves individual inflammatory cells consistent with neutrophils within the tissue, and no smudge, fold, air bubble, or stain precipitate obscures either view.

Scientific and Causal Correctness rubric:
level_1: The tissue looks non-biological (like fabric, paper, or plastic), the staining is a single flat color or has nuclei and cytoplasm colored backwards (pink nuclei, blue cytoplasm), and no cell shapes, tissue layers, or inflammatory cells are visible anywhere.
level_2: The tissue is biological and layered but the staining doesn't read as H&E (nuclei aren't blue-purple, cytoplasm isn't pink-red), cell outlines are indistinct, and even at the highest magnification shown, no neutrophils appear and the appendix's characteristic wall layering is not recognizable.
level_3: H&E coloring is correctly rendered (blue-purple nuclei, pink-red cytoplasm and stroma), the wall shows appendix-consistent layering (mucosa, submucosa, muscularis, serosa), and at high magnification, small multi-lobed dark-staining neutrophils are visible within the tissue layers themselves rather than confined only inside blood vessels.
level_4: Neutrophils specifically sit within the lamina propria and/or muscularis rather than only around vessels, they clearly outnumber any lymphocytes, plasma cells, or eosinophils in the infiltrate, and no necrotic or perforated-looking tissue is introduced anywhere in the section.
level_5: The density of neutrophils visibly increases from the submucosa toward the muscularis, at least one crypt in the mucosa is shown packed with neutrophils forming a crypt abscess, and nothing in the section resembles granulomas, tumor cells, parasitic eggs, or fibrosis that would point to a different diagnosis.

Spatiotemporal Consistency rubric:
level_1: The clip cuts abruptly between unrelated shots, the slide is missing from one frame and fully present in the next with no motion shown, the coverslip's glare or position changes suddenly with no cause, the tissue section jumps position or rotates with no motion shown, or the lighting swings sharply between bright and dark with no cause.
level_2: The tissue section shifts sideways by more than half its own width between the wide and narrow views, the coverslip's glare appears or disappears inconsistently between shots, the focus visibly hunts back and forth (sharp-blurry-sharp) rather than settling, or background elements like the stage clips or objective-lens rim change identity or position between shots.
level_3: The slide stays fully in frame and stationary, the tissue section stays centered throughout, the coverslip's reflection and mounting-medium sheen look the same in both views, the transition from the low-power to the high-power view is smooth and moves in one direction without backtracking, and the lighting's color and brightness stay even.
level_4: The microscope's stage and objective turret show no visible rotation or lateral shift, and the field of view narrows smoothly and evenly from all sides (not lopsided or with a hard crop appearing partway through) as the magnification increases, with no warping or distortion appearing during the transition.
level_5: The spatial relationships between tissue structures (for instance, the distance from the lumen to the mucosal surface relative to the muscularis) stay proportionally consistent as the magnification changes, scaling up evenly rather than stretching or shearing in one direction, and no flicker or motion blur disrupts the sense of one continuous zoom.