Domain: Engineering — Biomedical Engineering
Target concept: Biological Effects of Magnetic Field Intensity

Video generation prompt: Place the culture dishes containing rat vascular endothelial cells in the low-frequency pulsed electromagnetic field device and activate the device. The left culture dish was placed under a device with a magnetic field strength of 0 mT, the middle culture dish was placed under a device with a magnetic field strength of 20 mT, and the right culture dish was placed under a device with a magnetic field strength of 50 mT. After 24 hours of cultivation, cell proliferation was observed under a microscope. The microscopic fields of view from the three culture dishes were displayed side by side, with proliferative areas marked by orange fluorescence.

Evaluation guide:
(i) Target concept: Dose-dependent proliferative response of rat vascular endothelial cells to low-frequency pulsed electromagnetic field intensity.
Minimum mechanism: Proliferation marker (e.g., EdU or Ki-67) must be incorporated during exposure and detected via orange-fluorescent secondary antibody; fluorescence intensity/area must scale with field strength due to enhanced Ca²⁺ influx and ERK pathway activation, requiring strict spatial segregation of field conditions and controlled imaging timing.
(ii)Storyline: [Frame 0–2] Bench view: three dishes placed left/middle/right; devices lowered onto each dish; devices activate (subtle indicator light or pulse animation). [Frame 3–5] Transition to stabilized microscope view: three identical FOVs snap into fixed side-by-side alignment; background darkens uniformly. [Frame 6–10] Fluorescence emerges synchronously across all fields, then resolves into graded orange signal: left field remains near-background, middle develops discrete fluorescent clusters, right fills with dense, confluent orange signal, all strictly confined within cell layer boundaries; no motion, repositioning, or lighting change occurs during this phase. Perspective: static overhead microscope viewpoint; full visibility of all three fields and their relative signal differences at all times.

Manual:
Experimental Procedure
1. Place three identical culture dishes containing rat vascular endothelial cells on bench.
2. Position left dish under 0 mT device, middle under 20 mT device, right under 50 mT device.
3. Activate all devices simultaneously.
4. After 24h, image each dish under fluorescence microscope using same settings.
5. Display three microscopic fields side-by-side, with orange fluorescence marking proliferating cells.
Observation of Experimental Phenomena
Side-by-side comparison shows increasing orange fluorescence area/brightness: left (0 mT) ≈ minimal, middle (20 mT) ≈ moderate, right (50 mT) ≈ maximal — all confined to cell monolayers.
Summary of Experimental Principles
Low-frequency pulsed electromagnetic fields promote endothelial cell proliferation in dose-dependent manner; higher magnetic field intensity (0 → 20 → 50 mT) correlates with increased DNA synthesis and mitotic activity, visualized via proliferation marker fluorescence.

Prompt Grounding rubric:
level_1: No culture dishes visible; no electromagnetic field device present; the three dishes (or their microscope fields) are not shown arranged side by side, e.g., only one or two are shown, or they are stacked/overlapping; no microscope view; no orange fluorescence anywhere.
level_2: At least one culture dish appears, but missing at least two of: (a) three distinct dishes in left/middle/right positions, (b) electromagnetic field device(s) shown near dishes, (c) side-by-side microscopic field-of-view layout, (d) any orange fluorescence.
level_3: Three culture dishes are visibly placed left/middle/right in a single frame; a device is present adjacent to each dish; side-by-side microscopic fields are displayed; orange fluorescence appears in at least one field, but fluorescence is not consistently assigned to correct magnetic field strength positions (i.e., no fixed mapping between left=0 mT, middle=20 mT, right=50 mT).
level_4: Three culture dishes are placed left/middle/right; electromagnetic field devices are visibly associated with each dish (e.g., labeled or spatially anchored); side-by-side microscopic fields are shown; orange fluorescence appears in all three fields, and the fluorescence intensity pattern shows a clear left < middle < right progression (i.e., increasing brightness or area), but no explicit labeling or visual cue confirms the 0/20/50 mT assignment per position.
level_5: Three culture dishes are placed left/middle/right; each is unambiguously paired with its corresponding electromagnetic field device (e.g., device visibly overlaid or labeled with “0 mT”, “20 mT”, “50 mT”); side-by-side microscopic fields are simultaneously displayed in fixed alignment; orange fluorescence appears in all three fields with strictly increasing visual prominence (area or brightness) from left to right, and the leftmost field shows minimal/no orange signal, middle shows moderate, right shows maximal, with no deviation in spatial order, labeling, or fluorescence assignment.

Scientific and Causal Correctness rubric:
level_1: Fluorescence appears in wrong context (e.g., on device, in air, on non-cell surfaces); no proliferation-related signal; orange color absent or replaced by incorrect color (e.g., green, blue, red); fluorescence appears before activation or without device presence.
level_2: Orange fluorescence appears, but only in one field and unrelated to magnetic field strength; or fluorescence appears in all fields but with identical intensity/area across all three; or fluorescence appears in reverse order (right < middle < left) with no biological justification shown.
level_3: Orange fluorescence appears in all three side-by-side microscopic fields and is confined to cell-populated regions (not background); the right field is clearly more fluorescent than the left field, and the middle field's fluorescence falls somewhere between the two; the middle-vs-right or middle-vs-left distinction may be subtle (e.g., middle looks close in brightness to one of its neighbors), as long as the left field is clearly the dimmest of the three and the right field is clearly the brightest.
level_4: Orange fluorescence appears exclusively within cell-populated regions in all three fields; fluorescence area/brightness increases strictly left → middle → right, with each field clearly distinguishable from its neighbors; no fluorescence appears outside cell layers; no false-negative (e.g., zero signal in the right field) or false-positive (e.g., signal in the 0 mT dish exceeding either treated dish) violations, but the left field may retain a faint baseline signal, so long as it stays visually indistinguishable from background/autofluorescence, consistent with minimal proliferation at 0 mT.
level_5: Orange fluorescence is present only in cell monolayers; left field shows negligible or absent orange fluorescence (visually indistinguishable from background autofluorescence); middle field shows clearly greater orange fluorescence than left; right field shows clearly greater orange fluorescence than middle; no fluorescence appears in device hardware, media, or empty areas; no merging, bleeding, or halo artifacts distort spatial localization of signal; fluorescence distribution matches expected endothelial cell morphology (cobblestone pattern) in all fields.

Spatiotemporal Consistency rubric:
level_1: Severe spatiotemporal violation: one or more culture dishes teleport to a new position or size between frames; the field-of-view jumps to a different magnification or angle with no visible transition; a dish or its device flickers, disappears, or reappears; or the orange fluorescence in a field appears or disappears instantly with no visible ramp-up or fade.
level_2: Moderate violations: (a) the three dishes' left/middle/right order changes partway through the video; (b) a device separates from its dish, or ends up over the wrong dish, mid-sequence; (c) the three side-by-side microscope fields collapse into an overlapping or stacked layout instead of staying side by side; (d) the orange fluorescence in one or more fields flashes on for only a frame or two rather than staying visible.
level_3: All three dishes stay in their left/middle/right positions, and each device stays over its own dish, for the whole video; the three microscope fields stay arranged side by side without overlapping or swapping places; once orange fluorescence appears in a field it stays visible in all three fields for the remainder of the video, but the field borders show slight jitter, or the background brightness behind one dish drifts noticeably over time.
level_4: All 3-point criteria satisfied; plus: (a) the three dishes and their devices show no drift, rotation, or resizing at all; (b) the background behind each of the three fields stays evenly lit and unchanging for the whole video, so any change in orange signal is attributable to fluorescence rather than a background shift; (c) fluorescence stays visible in all three fields for the full observation window, but one field may show a single brief flicker in its fluorescence brightness or a slightly soft edge where the fluorescent region meets the unstained background.
level_5: All 4-point criteria satisfied; plus: (a) the three fields keep the same size, spacing, and left-to-right alignment relative to each other for the entire video, with no visible resizing or repositioning of any field; (b) the background behind all three fields stays equally dark and even throughout, with none of the fields growing brighter or dimmer for a reason unrelated to the orange fluorescence itself; (c) fluorescence appears at the same time across all three fields and stays steadily visible, without flicker, fading, bleeding across field boundaries, or being blocked, for the entire video.