Domain: Natural Science — Ecology & Evolutionary Biology
Target concept: Leaf closure response of Mimosa pudica following tactile stimulation

Video generation prompt: A potted Mimosa plant, after a human finger gently touches one of the leaflets on a compound leaf, is presented in a stable close-up shot.

Evaluation guide:
(i) Target concept: Thigmonastic leaf closure in Mimosa pudica mediated by pulvinus-driven turgor loss.
Minimum mechanism: Contact must trigger localized, reversible, downward folding of leaflets via pulvinus flexure, not passive wilting, not damage-induced necrosis, not hydraulic failure beyond recovery window.
(ii)Storyline: [Phase 1] Stable close-up reveals intact compound leaf with multiple leaflets in natural extended posture; [Phase 2] Bare finger enters frame and makes unambiguous, static contact with exactly one leaflet; [Phase 3] Within observable time, contacted leaflet initiates downward folding at its base (pulvinus hinge); [Phase 4] Folding propagates sequentially to neighboring leaflets on the same pinna: no skipping, no simultaneity, no reversal; [Phase 5] All folded leaflets remain stably closed with no rebound, while non-stimulated leaves/pinnae remain unchanged. Perspective must maintain fixed close-up framing throughout; leaflet bases must remain visible to confirm hinge-based motion, not mid-blade bending or detachment.

Manual:
Experimental Procedure
1. Place a healthy potted Mimosa pudica under steady ambient light.
2. Position camera for stable close-up framing of one compound leaf with visible leaflets.
3. Gently touch a single leaflet on that compound leaf with bare human fingertip—no pressure, no sliding.
Observation of Experimental Phenomena
Within seconds, the contacted leaflet folds downward along its midrib; adjacent leaflets on the same pinna sequentially fold downward in wave-like propagation; no unfolding occurs during observation.
Summary of Experimental Principles
Mimosa pudica exhibits rapid thigmonastic movement via turgor loss in pulvini at leaflet bases; tactile stimulation triggers ion flux, water efflux, and reversible loss of rigidity—demonstrating adaptive anti-herbivory response in plant evolutionary ecology.

Prompt Grounding rubric:
level_1: No potted Mimosa pudica plant is visible, no human finger appears in frame, no compound leaf with leaflets is shown, no finger-to-leaflet contact event occurs, or the shot is not a stable close-up (visible zooming, panning, heavy blur, or a distant framing that leaves leaflet detail unresolvable).
level_2: A potted plant and a finger are both present, but at least one setup element is wrong: the leaves lack the fine, bipinnate, multi-leaflet structure characteristic of Mimosa pudica, the finger contacts the stem, petiole, or soil instead of a leaflet, or the framing drifts, shakes, or cuts noticeably during the action.
level_3: A potted Mimosa pudica with clearly bipinnate compound leaves is shown; a human finger touches one leaflet; the framing is a stable close-up that keeps at least one full compound leaf and the touched leaflet in view; nothing blocks the touch point.
level_4: Beyond the 3-point requirements, the fingertip is shown making direct contact with no visible gap or shadow-only proximity, the pot is intact with visible soil, the compound leaf sits in its natural upright orientation rather than inverted or pressed flat, and no captions, watermarks, or overlays appear on screen.
level_5: Beyond the 4-point requirements, the finger is bare with no glove or tool, it touches exactly one leaflet without also brushing the rachis or a neighboring leaflet, and that leaflet's pinnules are clearly resolved as part of a single intact compound leaf, leaving no doubt about which leaflet was touched or whether the leaf is undamaged.

Scientific and Causal Correctness rubric:
level_1: No leaflet movement follows the contact; leaflets open or spread rather than close; every leaflet on the leaf collapses at once with no order of propagation; leaflets fold upward or invert instead of folding downward at the base; or folding begins before the finger has touched the leaf.
level_2: Leaflets move, but the biology is wrong: they curl or roll rather than folding flat at the base, the whole leaflet blade bends instead of hinging at the pulvinus, the folding starts before contact occurs, or the movement seems unconnected to any contact event.
level_3: After the finger touches one leaflet, that leaflet folds downward from its basal pulvinus, and at least one neighboring leaflet on the same pinna folds a moment later; the response spreads outward from the touched leaflet rather than collapsing the whole leaf simultaneously.
level_4: Beyond the 3-point requirements, every folding leaflet closes only downward, never buckling upward or twisting sideways; the leaflet blades keep their normal shape as they fold, with no kinking or shearing along the blade; and none of the folded leaflets reopens before the sequence ends.
level_5: Beyond the 4-point requirements, the fold spreads leaflet by leaflet along the pinna in one consistent direction away from the touch point with no leaflet skipped; no leaflet elsewhere on the plant folds on its own beforehand; and each fold visibly hinges at the swollen pulvinus at the leaflet's base rather than the blade creasing or tearing.

Spatiotemporal Consistency rubric:
level_1: Severe spatiotemporal break: the finger appears in frame with no entry motion, a leaflet vanishes and reappears, the pot's position jumps between frames, the light shifts abruptly from bright to dark with no cause, the background warps, or the folding motion is visibly discontinuous (jitter, ghosting, or duplicated frames).
level_2: Noticeable inconsistency: the finger's contact point shifts to a different leaflet with no transitional motion, a leaflet's position jumps rather than folding smoothly, the pot rotates or tilts for no reason, a shadow becomes detached from the object casting it, or the light visibly flickers across frames.
level_3: The viewpoint and layout stay stable: the pot stays in place, the finger's approach is smooth and continuous, leaflets change position only through the folding motion itself, and there is no teleporting, no warped geometry, no sudden lighting jump, and no background element blinking in or out.
level_4: Beyond the 3-point requirements, the finger's approach and the leaflets' folding both proceed with no frozen frames, no reversal, and no skipped stage of the fold; the finger's relative depth in front of the leaf stays consistent, and the leaflet edges and rachis line up from frame to frame without misalignment.
level_5: Beyond the 4-point requirements, the pot rim, soil surface, and leaf veins show no visible shake or drift when the plant is otherwise still, there is no visible strobing during the fold, the finger's occlusion of the leaf stays consistent as it moves, and no highlight slides across the leaf surface in a way that contradicts a fixed light source.