Domain: Healthcare — Microbiology-Immunology
Target concept: Detection of Alcohol Disinfection Effectiveness (Bacterial Simulation Method)

Video generation prompt: Place two agar plates on the lab bench. Inoculate both plates with simulated bacteria using a cotton swab. Apply 75% alcohol to one plate using a cotton swab, while leaving the other plate untreated. Cover both plates, then show an accelerated time-lapse of the standard overnight incubation period before revealing the comparison between the two plates.

Evaluation guide:
(i) Target concept: Visual demonstration of differential bacterial survival following localized alcohol disinfection on solid culture medium, made visible only after a real incubation period.
Minimum mechanism: Simulated bacteria must be viable and culturable pre-treatment; alcohol must make direct physical contact with the inoculated agar surface; inhibition requires sufficient contact time and concentration (75%) to prevent replication; colony formation itself requires a standard incubation period (about 18-24 hours at 35-37°C) before any growth becomes visible, so this time gap must be depicted as an explicit accelerated time-lapse or clearly labeled transition rather than shown as instantaneous; once visible, colony absence must spatially correspond to the alcohol contact zone.
(ii)Storyline: [Phase 1: Setup] Two agar plates placed adjacent on bench, lids closed → [Phase 2: Preparation] Lids opened simultaneously, revealing uniform pale-yellow/cream agar surfaces → [Phase 3: Inoculation] Cotton swab strokes entire surface of each plate in consistent motion → [Phase 4: Intervention] Second swab dips into visible 75% alcohol container, then contacts only one plate's surface → [Phase 5: Incubation Time-Lapse] Both lids close fully; an explicit accelerated time-lapse cue (e.g., a fast-forwarding clock or a day/night cycle dissolve) conveys the passage of the standard overnight incubation period → [Phase 6: Contrast Reveal] untreated plate is shown with dense colonies, treated plate shows a colony-free zone matching the swab contact area, all observed from a fixed frontal viewpoint with full plate visibility throughout except during the labeled time-lapse transition.

Manual:
Experimental Procedure
1. Place two identical agar plates side-by-side on clean lab bench.
2. Open lids. Use cotton swab to inoculate entire surface of both plates evenly.
3. Close lids. Dip new cotton swab into 75% alcohol solution. Apply swab firmly to surface of one plate through its lid opening.
4. Close lid of treated plate. Leave both plates covered and incubate at 35-37°C for 18-24 hours, shown as an accelerated time-lapse rather than in real time.
Observation of Experimental Phenomena
After the incubation period, the untreated plate shows dense, discrete bacterial colonies across the agar surface. The treated plate shows no colonies in the central region where alcohol was applied; peripheral colonies may remain.
Summary of Experimental Principles
75% alcohol denatures bacterial proteins and disrupts membranes on contact, but visible colonies only appear once surviving cells have had time to replicate — hence a standard incubation period is required before any growth difference becomes observable. Contact time, alcohol concentration, and the subsequent incubation period together determine the visual outcome of this disinfection-efficacy demonstration.

Prompt Grounding rubric:
level_1: No agar plates are visible; no cotton swab is present; no alcohol application action is shown; the two plates are never shown together in the same scene; nothing in the clip resembles an inoculation or comparison setup.
level_2: Two flat circular objects resembling plates sit on the bench, but at least one has the wrong shape (e.g., rectangular or irregular) or is missing its lid; a cotton swab appears but is never actually touched to a plate surface for inoculation or alcohol application; nothing distinguishes which plate, if either, was treated.
level_3: Two matching agar plates with clear lids sit side by side on a lab bench; a cotton swab visibly touches the surface of each plate in turn; a second, visibly wetted swab subsequently touches the surface of only one of the two plates; nothing else in the frame contradicts this sequence, even though no label marks which plate is which.
level_4: Everything described above still holds, and in addition: the treated plate carries a visible, lasting marker (e.g., a small '+Alcohol' label or a strip of colored tape on its lid) or the wetting swab is clearly dipped from a container labeled '75% Alcohol' immediately before touching the plate; the untreated plate carries no such marker and is never touched by that labeled container.
level_5: Everything described above still holds, and in addition: the '75% Alcohol' container is visibly in frame while the swab is dipped into it; both plates are shown with lids fully closed (no gap or tilt) immediately after inoculation and alcohol application and remain fully closed until the two plates are compared; no unrelated tools, hands, or labels appear anywhere in the shot.

Scientific and Causal Correctness rubric:
level_1: After the time-lapse, neither plate shows any bacterial growth pattern at all; both plates look uniformly blank; there is no visible contrast between the two plates and nothing links the alcohol application to any growth difference.
level_2: After the time-lapse, both plates show colonies of identical density, size, and distribution: the alcohol-treated plate grows just as heavily as the untreated one, with no zone of reduced growth anywhere.
level_3: After the time-lapse, the untreated plate shows visible, discrete, roughly circular colonies spread across the agar surface, while the treated plate shows clearly fewer colonies overall (fewer than half the colony count of the untreated plate, or growth confined mostly to the plate's edge with the middle mostly bare), even though the bare region on the treated plate does not yet have a sharp, clean edge.
level_4: In addition to the pattern above, the treated plate's central region (roughly the middle two-thirds of the plate) is essentially free of colonies, with any surviving colonies confined to the outer rim, while the untreated plate's colonies have grown together into a dense, largely continuous layer covering most of the surface; nothing on either plate looks like mold or an implausible colony texture.
level_5: In addition to the pattern above, the bare central region on the treated plate has a crisp, clearly defined edge rather than a fuzzy gradient, and its shape and position roughly track where the alcohol-soaked swab made contact; the untreated plate's colonies remain individually distinguishable rather than merged into an undifferentiated haze across the rest of its surface; the area the swab actually touched on the treated plate shows essentially zero colony growth.

Spatiotemporal Consistency rubric:
level_1: One of the two plates vanishes from its spot and reappears elsewhere with no hand ever shown moving it; the cotton swab or a lid appears or disappears between frames with no motion shown; a plate flips or rotates with no visible cause; the bench lighting flips abruptly between bright and dark with no in-scene cause outside of the labeled incubation time-lapse; or the plates cut directly from being freshly covered to showing full colony growth with no time-lapse, dissolve, or other transition cue depicted at all, making the bacterial growth appear to occur instantaneously.
level_2: A plate visibly shifts its position on the bench (more than roughly a centimeter) between the inoculation step and the alcohol step without any hand visibly moving it; the swab's angle jumps abruptly (e.g., a roughly 90-degree turn) between frames instead of moving smoothly; a lid shown open during inoculation appears closed in the very next frame with no closing motion shown.
level_3: Both plates stay in the same spot relative to each other and the bench throughout every step; the swab moves smoothly from one plate to the other, then to the alcohol container, then to the treated plate, without jumping around; each lid opens once before inoculation and closes once after the alcohol step, and both motions are fully shown.
level_4: Everything above holds, and in addition: the hand and swab never fully hide the plate surface from view during inoculation or alcohol application except during the explicitly depicted incubation time-lapse; the camera framing does not pan, tilt, or zoom outside of that time-lapse transition; the bench lighting looks the same immediately before the time-lapse begins and immediately after it ends, once real-time action resumes.
level_5: Everything above holds, and in addition: the sequence of events follows this order without being reordered or repeated: plates placed side by side, lids opened, first plate inoculated, second plate inoculated, alcohol swab wetted, treated plate contacted, both lids closed, an explicit accelerated time-lapse (clearly marked, e.g. a dissolve, a fast-forwarding clock, or a day/night cue) conveys the incubation period, then both plates are shown for comparison; that one deliberate time-skip at the incubation step is the only discontinuity in an otherwise unbroken sequence.