Domain: Healthcare — Pathology
Target concept: Inflammation (acute inflammatory cell infiltration)

Video generation prompt: An acute inflammatory tissue section is placed on the experimental table, and cellular infiltration is observed under the optical microscope.

Evaluation guide:
(i) Target concept: Acute inflammatory cell infiltration as a histopathological signature.
Minimum mechanism: Neutrophils must be present, morphologically intact, and spatially localized in tissue parenchyma or around vessels, requiring intact tissue fixation, proper H&E staining, and optical magnification sufficient to resolve nuclear segmentation and cytoplasmic granularity.
(ii)Storyline: [Phase 1: Setup] Tissue slide placed flat on table surface → [Phase 2: Alignment] Microscope lowered/positioned with objective centered over slide → [Phase 3: Observation] Eyepiece view or observer posture indicates active viewing → [Phase 4: Phenomenon] Microscopic field reveals neutrophils clustered near vessels/interstitium, distinct from background tissue. All phases occur within fixed viewpoint, no camera cuts, no zoom transitions, no external annotations or overlays; visibility constrained to what is optically resolvable at 10×–40× magnification.

Manual:
Experimental Procedure
1. Place H&E-stained acute inflammatory tissue section on glass slide onto experimental table.
2. Position optical microscope directly above slide with objective lens aligned vertically.
3. Observe under microscope at medium power (10× or 40× objective).
Observation of Experimental Phenomena
Neutrophils with multi-lobed nuclei and pale granular cytoplasm infiltrating interstitial and perivascular spaces; background tissue architecture preserved; nuclei stained blue-purple, cytoplasm and collagen stained pink.
Summary of Experimental Principles
Acute inflammation is characterized by rapid neutrophil recruitment into affected tissue, visible histologically as dense, localized infiltration — a hallmark diagnostic feature in pathology.

Prompt Grounding rubric:
level_1: The experimental table, optical microscope, and tissue slide are not shown together, or the scene depicts something unrelated to a microscopy setup (e.g., a patient bedside, a chemistry bench with flasks/reagents, or a bare digital display); none of the three core objects named in the prompt (table, microscope, slide) are jointly present.
level_2: Only one of the three core objects (experimental table, optical microscope, tissue slide) is clearly shown, or two appear but never together in the same shot; or the microscope is shown positioned/focused before the slide is ever placed on the table (reversed action order); or no viewing/observation action (an eye at the eyepiece, or the camera oriented toward the ocular) is ever depicted.
level_3: All three core objects co-occur on screen: the experimental table, the optical microscope resting on it, and the tissue slide; the slide is placed onto the table before the microscope is brought into position above it; the observation step (an implied eye at the eyepiece, or the ocular lens shown in a viewing orientation) follows the positioning step; the order placement → positioning → observation is never reversed.
level_4: In addition to the 3-point requirements: the microscope objective lens is centered directly over the slide along a shared vertical axis rather than offset to one side; the eyepiece/ocular tube sits upright in a focus-ready orientation with no visible tilt, detachment, or loose components on the microscope body; and no core object is missing, duplicated, or swapped for an unrelated item (e.g., no petri dish or beaker standing in for the glass slide).
level_5: In addition to the 4-point requirements: the slide sits fully seated and flush in the stage clips/holder (not floating, tilted, or hanging off the stage edge); and the whole sequence (table, slide, microscope, and observation) is rendered from a single fixed camera viewpoint with no cutaways, inserted close-ups, or viewpoint jumps.

Scientific and Causal Correctness rubric:
level_1: No cells resembling an acute inflammatory infiltrate are visible: the tissue looks histologically normal, necrotic, purely fibrotic, or neoplastic instead; or the staining palette contradicts standard H&E convention (e.g., nuclei rendered pink/red instead of blue-purple, or the background tinted an unnatural color instead of eosinophilic pink).
level_2: Cells are present but the population is wrong for acute inflammation: e.g., lymphocytes or macrophages dominate the field instead of neutrophils, or the visible nuclei are uniformly dense and clumped (a chronic-inflammation pattern) rather than segmented and multi-lobed; and/or the cells are spread uniformly through the tissue with no clustering near vessels or in interstitial spaces.
level_3: Neutrophils are clearly the dominant infiltrating cell type, recognizable by multi-lobed (roughly 2-5 lobe) nuclei and pale, finely granular cytoplasm, and they cluster in interstitial or perivascular locations rather than being scattered evenly; the surrounding tissue architecture (muscle fibers, gland structures, or connective stroma) remains identifiable rather than being obliterated; H&E color fidelity holds: nuclei blue-purple, cytoplasm/collagen pink, red blood cells orange-red.
level_4: In addition to the 3-point requirements: neutrophil density is visibly elevated compared to a normal, non-inflamed baseline: the infiltrate reads as a clear cluster or band rather than a few isolated cells; no processing artifacts are present that could be mistaken for cells (e.g., tissue folds, chatter marks, or stain precipitate); and red blood cells, where visible, are intact (not lysed or ghosted) and appropriately smaller than the neighboring neutrophils.
level_5: In addition to the 4-point requirements: the field of view is dominated essentially exclusively by neutrophils, with no substantial presence of other inflammatory or abnormal cell types (no lymphocyte-rich, plasma-cell-rich, eosinophil-rich, or tumor-cell regions competing for attention); and this dense, localized neutrophilic pattern is sustained for as long as the tissue stays in view rather than reverting to a normal-looking, non-infiltrated appearance.

Spatiotemporal Consistency rubric:
level_1: Severe spatiotemporal breakdown: the slide appears on the table without ever being placed (it snaps into position between frames with no hand or tool motion), the microscope's position or shape changes discontinuously (duplicating, vanishing, or its arm bending unnaturally), or the scene lighting switches on and off abruptly between frames with no transition.
level_2: Noticeable discontinuity: the slide's placement onto the table skips frames or shows no continuous contact from a hand or tool during the transition; the microscope shifts from one position to another without any intermediate motion shown; or the microscope's own body casts a shadow across the slide and tabletop that swings to a different side between two frames despite neither the microscope, the slide, nor the camera having moved.
level_3: Once placed, the slide stays centered under the microscope objective for the remainder of the sequence; the microscope base stays planted on the table without unexplained sliding, rotating, or floating; there are no camera cuts or jumps within the sequence; and the ambient lab lighting stays constant, so the microscope's shadow falls across the same side of the tabletop and slide, at a consistent length, throughout.
level_4: In addition to the 3-point requirements: any hand or tool motion (placing the slide, turning the focus knob) traces one smooth, continuous path in a single direction, without snapping backward or stuttering mid-motion; and the relative sizes and positions of the table, slide, and microscope stay proportionally consistent with each other as the fixed camera viewpoint holds, with no sudden shifts in relative scale between the foreground microscope and the background table surface.
level_5: In addition to the 4-point requirements: depth relationships among the microscope's components stay plausible throughout (for instance the ocular tube consistently reads as nearer/smaller than the objective turret from the given camera angle), and the edges of the slide, microscope, and table never double, warp, or momentarily misalign from one frame to the next.