Domain: Engineering — Food and Bioprocess Engineering
Target concept: Enzymatic Hydrolysis of Soy Protein: Determination of Hydrolysis Efficiency (Solubility Method)

Video generation prompt: Take two test tubes, add the unhydrolyzed soy protein suspension (containing precipitate) to tube A, and add the soy protein solution treated with neutral protease to tube B, then shake thoroughly to mix.

Evaluation guide:
(i) Target concept: Visual differentiation of solubility states driven by enzymatic hydrolysis efficacy.
Minimum mechanism: Soy protein must exist in two physically distinct colloidal states: one aggregation-prone (native, unhydrolyzed) and one molecularly dispersed (hydrolyzed), such that mechanical agitation does not alter their inherent phase stability; precipitate persistence in A and absence in B must reflect irreversible biochemical modification, not transient suspension.
(ii)Storyline: Frame 0–2: Static shot reveals two labeled tubes side-by-side: tube A shows settled white precipitate under cloudy supernatant; tube B shows uniform clarity with sharp meniscus. Frame 3–5: Hand enters, grasps tube A, shakes vertically/horizontally → precipitate disperses temporarily but re-aggregates visibly within 1 s. Frame 6–8: Hand grasps tube B, repeats identical shake → no particle disturbance occurs; liquid remains optically homogeneous. Frame 9–10: Final static hold confirms contrast: A retains coarse particulates; B remains particle-free. Perspective: Fixed frontal view, eye-level, shallow depth-of-field focusing on tube mid-sections; labels A/B always fully in-frame and legible; no background distraction.

Manual:
Experimental Procedure
1. Place two identical test tubes side-by-side.
2. Add unhydrolyzed soy protein suspension (white precipitate + cloudy liquid) to tube A.
3. Add neutral protease–treated soy protein solution (clear, homogeneous liquid) to tube B.
4. Shake both tubes thoroughly and separately.
Observation of Experimental Phenomena
Tube A: Persistent white precipitate remains visible before and after shaking (no solubilization).
Tube B: No precipitate present before or after shaking (complete solubilization due to enzymatic hydrolysis).
Summary of Experimental Principles
Enzymatic hydrolysis by neutral protease cleaves peptide bonds in soy protein, increasing solubility. Unhydrolyzed protein aggregates and precipitates; hydrolyzed peptides remain dispersed in solution. Solubility difference is visually assessed via presence/absence of precipitate after agitation.

Prompt Grounding rubric:
level_1: No test tubes are present; or the unhydrolyzed soy protein suspension (with visible precipitate) is missing from tube A; or the protease-treated soy protein solution is missing from tube B; or no shaking action is performed on either tube; or only one tube is shown at all.
level_2: Two test tubes are present, but their starting contents are mismatched (e.g., both tubes look identical before shaking, tube A shows no precipitate, or tube B looks turbid instead of clear); or shaking is applied to only one of the two tubes; or the tubes are not distinguishable as A and B (no labels, no consistent spatial order).
level_3: Two test tubes are clearly labeled or spatially designated as A and B; tube A initially contains a cloudy suspension with visible white precipitate; tube B initially contains a clear, homogeneous liquid; a shaking action (hand grips the tube and moves it) is applied to both tubes, in either order.
level_4: All of 3-score, plus: the tubes are shown side by side in one fixed shot; the A/B labels stay legible throughout; each tube is shaken individually, one at a time (not both waved together in a single motion); no unrelated object blocks the view of either tube or its label at any point.
level_5: All of 4-score, plus: each tube's shaking is clearly deliberate (a hand grips and agitates the tube rather than just bumping it), both tubes receive a comparable amount of visible agitation, and the two tubes keep their same left-right positions and labels throughout the entire shot with no swapping or relabeling.

Scientific and Causal Correctness rubric:
level_1: Tube A shows no precipitate at all (appears fully clear), or tube B shows a persistent precipitate (appears fully turbid), the opposite of the expected hydrolysis outcome; or shaking makes the precipitate vanish from tube A or a precipitate newly appear in tube B; or the two tubes look identical before and after shaking.
level_2: Before shaking, tube A correctly shows precipitate and tube B correctly shows none, but after shaking the precipitate in tube A dissolves away, or new cloudiness/precipitate appears in tube B; or shaking triggers an unrelated effect such as a color change, foaming, or the liquid separating into layers.
level_3: Tube A shows a cloudy suspension with visible white precipitate before shaking, and the precipitate is still present (possibly stirred up, then resettling) after shaking; tube B shows a clear, particulate-free liquid both before and after shaking; no unrelated effects such as foaming, discoloration, or layering occur in either tube.
level_4: All of 3-score, plus: the contrast between the tubes is unambiguous: tube A's precipitate is clearly coarser and more voluminous than any faint particulate that might appear in B, and tube B's liquid stays free of haze or graininess; no transient artifact (e.g., air bubbles stirred up by shaking) is mistaken for precipitate in either tube.
level_5: All of 4-score, plus: tube A's precipitate resettles again shortly after each shake with no lasting dissolution, tube B never develops any cloudiness or particulate at any point in the clip, and this contrast between the two tubes holds all the way to the end of the shot with no partial convergence toward a similar appearance.

Spatiotemporal Consistency rubric:
level_1: Tube A or tube B vanishes from its spot and reappears elsewhere without any visible motion in between; a tube's shape distorts unnaturally (bulges or warps); the A/B label flickers or jumps to a different position; the shaking hand appears mid-motion with no visible approach toward the tube; or a sudden, unexplained shift in lighting obscures the tube contents.
level_2: Both tubes stay roughly in place but show a noticeable jump or warp in perspective between moments; the shaking motion is jerky, with more than two visible stalls; the shadow cast by a tube switches sides abruptly; or one of the two tubes drops out of view for at least a second while the other stays visible.
level_3: Tube A and tube B keep the same side-by-side positions throughout; neither tube vanishes, warps, or breaks apart; the shaking motion for each tube is a single continuous gesture (hand enters, grips the tube, agitates it, withdraws) without unexplained jumps; the tube contents are never abruptly hidden mid-shake.
level_4: All of 3-score, plus: the camera viewpoint stays completely still (no pan, tilt, or zoom); the light on the tubes stays even, with no sudden brightening, dimming, or shift in where the shadows fall; each tube's cylindrical shape and the curve of its liquid surface (meniscus) look consistent from frame to frame; the shaking hand moves in a natural, single arc rather than snapping or stretching unnaturally, and the contents inside each tube begin visibly moving at the same moment the hand makes contact, with no lag between contact and response.
level_5: All of 4-score, plus: the motion throughout is smooth, with no jerky strobing or excessive blur that would make the tube contents hard to see; the liquid and precipitate inside each tube move the way real liquid would when shaken (sloshing, particles swirling and gradually resettling) rather than looking rigid or pasted-on; no glitchy edges, flicker in transparency, or doubled outlines appear on either tube.