Domain: Healthcare — Pathology
Target concept: Metaplasia (epithelial metaplasia)

Video generation prompt: A cervical mucosal tissue specimen subjected to prolonged chronic stimulation is placed on the experimental table, and the epithelium is examined under the microscope.

Evaluation guide:
(i) Target concept: Epithelial metaplasia in cervical mucosa as a reversible adaptive response to chronic irritation.
Minimum mechanism: The tissue specimen must visibly carry two adjacent, contiguous surface zones (a redder, columnar-type region and a paler, squamous-type region), so the transformation implied by chronic stimulation is legible from the specimen's own appearance, without relying on a microscope close-up that the shot never actually provides.
(ii)Storyline: [Frame 0–2] The cervical mucosal tissue specimen is carried onto the experimental table and set down. → [Frame 3–5] The upright microscope is positioned so the specimen sits centered on its stage, objective lowered over the tissue. → [Frame 6–10] The observer leans in and aligns one eye with the eyepiece, as if actively examining the mounted specimen. Perspective: A fixed overhead or slightly oblique shot keeps the table, specimen, and microscope together in one continuous view. Visibility: The specimen stays fully visible and unobscured on the stage; the microscope stays fully assembled and recognizable; there is no cut to a magnified histology view; everything about the tissue's zones must be readable from the specimen's own surface, the setup, and the observer's behavior.

Manual:
Experimental Procedure
1. Place cervical mucosal tissue specimen on experimental table.
2. Position upright microscope so its stage holds the specimen, objective aligned over the tissue.
3. Examine the mounted specimen with the microscope in place; no magnified/histological view is shown on screen — the setup and the specimen's own surface are what the video shows.
Observation of Experimental Phenomena
Two adjacent zones are visible on the specimen's own surface, forming one continuous, irregular boundary: a redder, glossier/velvety region (grossly consistent with columnar mucosal epithelium) and a paler, smoother region (grossly consistent with squamous epithelium).
Summary of Experimental Principles
Chronic stimulation induces epithelial metaplasia: replacement of native columnar epithelium by stratified squamous epithelium — a reversible adaptive change in cervical mucosa. At the cellular level this reflects columnar cells (tall, narrow, vertically oriented nuclei) being replaced by squamous cells (flattened, pale cytoplasm); this cellular-level detail is background principle inferred from the gross appearance, not something the video shows directly.

Prompt Grounding rubric:
level_1: No cervical tissue specimen appears anywhere in the shot, no microscope is present, no experimental table is shown, and neither a placement action nor an examination action takes place.
level_2: The tissue specimen and the microscope are not shown together as one working setup: for instance, the specimen never actually gets placed on the table, or the microscope is present but never turned toward the specimen, or only one of the two actions (placing the specimen, then examining it) happens while the other is skipped.
level_3: The tissue specimen is placed onto the experimental table, and an upright optical microscope with a stage and eyepiece is present and aimed at the specimen; placing the specimen clearly happens before any examination begins; the specimen reads as a thin, pinkish-tan mucosal fragment rather than an unidentifiable blob, and the microscope reads as a real bench microscope rather than a generic box.
level_4: Beyond simply being nearby, the specimen sits centered directly on the microscope's stage, and the observer's eye is clearly aligned with the eyepiece (visible in profile or from the front, with eye, ocular lens, and mounted specimen roughly on one sightline), showing the person is actually looking through the instrument at the tissue.
level_5: One continuous camera view holds the table, the specimen, and the microscope together for the whole shot with no cut to a different angle; the specimen stays put on the stage and the microscope stays in its examining position for the remainder of the clip; no stray hand, tool, or glare blocks the specimen or the stage area.

Scientific and Causal Correctness rubric:
level_1: The specimen's surface reads as one uniform color and texture from edge to edge; nothing about its appearance suggests that one part of the tissue differs biologically from another.
level_2: The specimen shows some discoloration or patchiness, but it reads as a random blemish, lighting glare, or staining artifact rather than two distinct, bounded biological zones; e.g., one blotch, one bright spot, or a gradient that never resolves into two separate regions.
level_3: The specimen's surface shows two adjacent, contiguous regions on the same piece of tissue: one region is redder and glossier or velvety-looking (consistent with columnar mucosal epithelium seen with the naked eye), and the other is paler pink and smoother or matte (consistent with squamous epithelium).
level_4: The boundary between the redder, glossier region and the paler, smoother region forms one continuous, irregular line rather than a sharp ruled edge or a flat color block, and the microscope's stage and objective are positioned so this boundary (not an unrelated patch of tissue) sits directly under the eyepiece where the observer is looking.
level_5: The redder region additionally shows faint mottling or fine speckling consistent with small underlying vessels or gland openings, while the paler region looks more even and slightly thicker or duller, so the whole specimen reads as a genuine transformation zone gradually shifting from columnar to squamous character rather than two flat colors placed side by side.

Spatiotemporal Consistency rubric:
level_1: The specimen appears on the microscope stage without ever having been carried there from the table, or the microscope itself disappears and reappears within the shot, or the tissue's outline suddenly snaps into a different shape with no motion connecting the two states.
level_2: One clear continuity break occurs: for example, the specimen is already sitting on the stage in the very first frame with no placement action shown, the microscope's orientation suddenly jumps by a quarter turn between moments, or the room's lighting flips from bright to dim with no visible cause.
level_3: The hand carrying the specimen moves smoothly from off-frame onto the table and comes to rest, the specimen then stays put until it is moved onto the stage, and the microscope does not shift position once it is set up to examine the tissue; nothing in the shot jumps location or appears/disappears without a visible cause.
level_4: As the hand carries the specimen and later the observer leans toward the eyepiece, the hand and head pass behind the microscope's arm or base wherever the geometry calls for it rather than floating in front of parts they should be behind, and the specimen's outline changes only through plausible handling (being set down, nudged into place) rather than through sudden reshaping.
level_5: Across the entire shot, the specimen keeps the same position relative to the stage and the microscope keeps the same position relative to the table once each is set in place (neither drifts or slides on its own), and reflections in the eyepiece shift smoothly with the observer's head movement rather than popping abruptly between states.