Domain: Healthcare — Microbiology-Immunology
Target concept: Giemsa Staining (Mycoplasma Observation)

Video generation prompt: Place a slide with fixed cell smears, add a drop of diluted Giemsa stain using a pipette, stain the cells, then gently rinse with buffer water, air dry the slide, and observe under an optical microscope. The entire procedure is continuous and simplified; a stylized, representative magnified view of the stained cells is sufficient, and exact microbial dimensions are not required.

Evaluation guide:
(i) Target concept: Visual demonstration of Giemsa staining protocol for Mycoplasma detection in fixed cell smears.
Minimum mechanism: Aqueous dye solution must make physical contact with fixed biological material, undergo selective binding to nucleic acids, survive controlled removal of unbound fraction, retain bound complex through desiccation, and yield optically resolvable contrast under transmitted light microscopy; because true Mycoplasma (roughly 0.2–0.8 µm) are at or below what a short generated video can resolve with verifiable precision, a stylized, representative view of small stained bodies distinct from the background is an acceptable stand-in for exact microbial morphology.
(ii)Storyline: Frame 1–2: Static overhead view of clean glass slide bearing a faint, off-white elliptical smear region. Frame 3–4: Pipette enters frame from top-right, descends steadily, and deposits a single cohesive purple-blue droplet centered on smear. Frame 5–6: Droplet spreads uniformly, intensifies in hue, then remains stable for ≥1 second. Frame 7–8: Clear buffer water flows laterally from left, covering entire stained area without overflow or pooling. Frame 9: Slide surface transitions from glossy to matte within smear region; no external contact. Frame 10: Seamless cut or focus ramp to microscope eyepiece view showing a stylized, representative view of small, discrete, basophilic Mycoplasma-like bodies against a neutral background; no transition artifacts, no perspective shift, no lighting break.

Manual:
Experimental Procedure
1. Place glass slide with fixed cell smears horizontally.
2. Using a pipette, deliver one drop of diluted Giemsa stain directly onto the smear.
3. Allow stain to act for visible contact time (no wiping).
4. Gently rinse smear with buffer water — flow must cover entire stained area.
5. Air-dry slide completely (no blotting/toweling).
6. Observe under optical microscope at 100× magnification.
Observation of Experimental Phenomena
Stained Mycoplasma appear as small (0.2–0.8 µm), round-to-ovoid, basophilic bodies with faint halos and no internal structure; nucleoid stains darker blue than cytoplasm; background is neutral gray with no nonspecific precipitation.
Summary of Experimental Principles
Giemsa stain binds DNA/RNA via azure B and eosin; Mycoplasma lack cell wall and stain poorly with Gram methods but retain Giemsa due to high nucleic acid content; fixation preserves morphology; buffer rinse removes unbound dye while preserving bound complex.

Prompt Grounding rubric:
level_1: None of the five required elements (the smear slide, the pipette, the Giemsa stain droplet, the buffer rinse, and the microscope view) appear on screen, or fewer than two of them appear at all.
level_2: At least one element appears (for example the slide or the pipette), but the required sequence of actions never happens: no droplet is delivered onto the smear, no rinse follows it, no drying is shown, and there is no final microscope view.
level_3: The smear slide, the pipette, the stain droplet, the buffer rinse, and the microscope view are all present, and they occur in the right order (slide first, then the pipette delivers a droplet onto the smear, then a visible rinse, then drying, then the microscope view), but at least one step is misconnected (for example the rinse appears to happen before the droplet lands, or the microscope view opens on an empty, unstained field).
level_4: Everything required for score 3 is shown, with each step correctly linked to the one before it: the pipette tip is positioned above the slide when the droplet lands on the smear, the rinse liquid visibly passes only over the stained area, drying is shown without any wiping or blotting, and the microscope view shows visibly stained cellular material; only incidental issues remain, such as the pipette being held at an odd angle or slight blur during the rinse.
level_5: Everything required for score 4 is shown, and in addition every element looks like what it is meant to be (the pipette has a distinguishable tip, the slide's smear area has a visible boundary, the stain droplet is a purple-blue color that spreads evenly over the smear, the rinse liquid is clear and flows without splashing), each action lands precisely where expected (the droplet centers on the smear, the rinse stream visibly originates off to the side of the slide and washes across the stained area, the drying is shown as a gradual loss of wetness rather than a sudden change), and the final microscope view shows multiple small, discrete stained bodies that are clearly distinguishable from the background and colored consistent with Giemsa staining.

Scientific and Causal Correctness rubric:
level_1: The stain shown is not a purple-blue color (e.g., it looks green, yellow, or red), the cells appear unstained or visibly disintegrate, the rinse washes away all color from the smear, no color develops on the smear at all, or the microscope view shows rod- or coccus-shaped bacteria rather than the small round-to-oval bodies expected for Mycoplasma.
level_2: The stain color is wrong (not purple-blue), or the stained smear region disappears entirely during the rinse, or the dried slide shows no remaining color at all, or the microscope view shows only dust, bubbles, or scratches with nothing resembling stained cells, and there is no sign of the characteristic Giemsa purple-blue contrast anywhere.
level_3: The droplet and the stained smear both appear purple-blue; the rinse clearly removes the excess unbound dye while leaving a visible purple-blue tint on the smear; the dried slide keeps that tint; and the microscope view shows small, round-to-oval stained bodies against the background; but the contrast is weak, for example each body looks uniformly colored with no darker central spot, or the background is tinted blue enough to make the bodies hard to pick out.
level_4: Everything required for score 3 holds, and in addition the color visibly builds up (the droplet starts as a lighter purple and darkens where it touches the smear, and that darker color survives the rinse and the drying), and the microscope view shows a visible contrast between a darker-staining center and a lighter surrounding area on each small body, with the bodies clearly separate from any debris or larger cell types; only a small imperfection remains, such as a faint blue cast to the background or two bodies sitting close enough to look joined.
level_5: Everything required for score 4 holds, and in addition the droplet's purple-blue color is unambiguous from the moment it touches the smear, the rinse leaves a crisp, high-contrast result (a darker-staining center clearly visible within each small stained body against a lighter surrounding area), the dried slide shows no blotchy or crystallized residue, and the microscope view shows multiple small, discrete stained bodies clearly separated from one another against a clean, neutral background with no stray specks of unexplained color.

Spatiotemporal Consistency rubric:
level_1: The slide instantly appears in a different position with no motion shown in between, the pipette appears or disappears mid-action, the stain droplet floats without the pipette or slide supporting it, the rinse liquid appears to flow upward, the view jumps straight from the tabletop to the microscope with no transition at all, or the overall lighting swings from bright to completely black in a single frame.
level_2: The smear's position on the slide shifts between frames even though nothing touched it, the pipette tip's position doesn't match where the droplet actually lands, the rinse liquid starts flowing from off-screen with no visible source, the slide's surface changes from wet to dry in a single instant with no gradual transition, or the shift into the microscope view happens as a sudden zoom or spin rather than any kind of smooth transition.
level_3: The slide stays in the same place on the surface throughout; the pipette moves smoothly from wherever it enters the frame to the slide; the droplet only appears once the pipette tip actually reaches the smear; the rinse liquid comes from a visible source and flows continuously across the smear; the slide's surface gradually loses its wet sheen rather than drying all at once; and the shift to the microscope view happens as a seamless cut or a visible focus change/eyepiece alignment, with no transition artifact, perspective jump, or lighting break, though one small hiccup is tolerated, such as a brief flicker right as the rinse begins.
level_4: Everything required for score 3 holds, and in addition the slide never shifts position or orientation across any of the steps, the pipette's path to the slide is smooth and never bends at an implausible angle, the droplet forms and spreads without any sudden distortion, the rinse liquid flows in one steady sheet from its source across the smear, the drying happens evenly across the stained area, and the entry into the microscope view is a clean seamless cut or a natural-looking focus change, with no transition artifact; only one small, non-disruptive imperfection is tolerated, such as a slight wobble in the pipette's motion.
level_5: Everything required for score 4 holds, and in addition the slide never visibly shifts position at all; the pipette decelerates smoothly to a stop right at the point of contact; the droplet appears already formed and spreads outward evenly with no spatter; the rinse begins only after the stain has visibly colored the smear for a moment and before any part of it has begun to dry at the edges, then flows steadily and stops cleanly; the drying front recedes evenly and symmetrically across the stained area; and the transition into the microscope view, whether a seamless cut or a gradual focus change, shows no artifact, centers on the stained region, and settles into a steady, clearly focused magnified image with no further jumps or unexplained cuts.