Domain: Healthcare — Microbiology-Immunology
Target concept: UV Sterilization Effectiveness Testing (Microbial Culturing Simulation)

Video generation prompt: Inoculate two agar plates with the same bacterial culture, then place them side by side. One plate stays covered as a control. The other has its lid removed and is exposed to ultraviolet light for a short period of time at a fixed distance. Its lid is then replaced, both plates are labeled, and both are incubated.

Evaluation guide:
(i) Target concept: Differential microbial survival under UV-C exposure, verified by comparing colony growth on two identically inoculated agar plates, only one of which was UV-treated.
Minimum mechanism: Both plates must receive the same microbial inoculum (e.g., S. aureus or E. coli) during an initial swabbing step, before their lids go on for the UV phase; the agar must support growth of that culture; the control plate's lid must stay on for the entire UV-exposure phase so no UV reaches its surface; the exposed plate's inoculated surface must receive direct, unobstructed UV-C flux for long enough to inactivate the culture.
(ii)Storyline: Frame 1: Two open agar plates sit side-by-side; a swab spreads the same bacterial suspension evenly across both plate surfaces. Frame 2: Lids are placed on both plates. Frame 3: The left lid stays on (control); the right lid is fully removed and set aside. Frame 4: A UV lamp activates, illuminating only the right plate's surface (violet-blue glow visible). Frame 5: The right lid is replaced. Frame 6: Both plates are labeled (text visible on lids or plates). Frames 7-10: Both plates stay covered and undisturbed, implying incubation - the final frame reveals the differential outcome (colonies on the left plate, none on the right). Perspective: fixed frontal view, both full plates in frame, no occlusion, consistent lighting and focus throughout.

Manual:
Experimental Procedure
1. Place two identical agar plates side-by-side on a clean surface, lids off.
2. Using a sterile swab, apply the same bacterial suspension evenly across the surface of both plates, then place a lid on each.
3. Keep the lid fully on the left plate (control). Remove the lid completely from the right plate (to be exposed).
4. Illuminate the exposed plate's surface only with a UV-C light source for a short duration.
5. Replace the lid on the exposed plate; label both plates clearly.
6. Incubate both plates under identical conditions.
Observation of Experimental Phenomena
After incubation: the control plate (inoculated, lid never removed for UV) shows abundant discrete microbial colonies across its surface; the exposed plate (inoculated, then UV-treated) shows zero colonies - a sterile-looking surface with the same texture and moisture as the control except for the total absence of growth.
Summary of Experimental Principles
UV-C radiation damages microbial DNA and blocks replication. Because both plates received the same bacterial inoculum before the right plate's lid was removed for treatment, the control plate's abundant colonies simply reflect normal growth of that inoculum, while the complete absence of colonies on the UV-treated plate shows that the UV-C exposure - not any difference in starting contamination - is what killed the microbes.

Prompt Grounding rubric:
level_1: No agar plates appear in the shot; there is no covered control plate and no UV-exposed plate; no UV light source is shown; the plates are never labeled and no incubation period is implied.
level_2: Only one plate is shown, or two plates appear but never together in the same shot; both plates are shown with lids on the whole time or both with lids off, so there is no covered-control-versus-exposed-plate distinction; a UV lamp is missing, or its beam falls on empty bench space rather than on a plate.
level_3: Two agar plates sit side by side in the same shot; a swab or loop is shown spreading liquid across both plate surfaces before either plate is covered for the UV phase; afterward, the left plate's lid stays on (control) while the right plate's lid is fully removed and a UV lamp with a visible violet-blue glow sits over its open surface; the right lid is put back on, then both plates are labeled, and the final shot shows both plates covered and undisturbed.
level_4: Everything required for score 3 is shown AND: the same swabbing motion and liquid appearance is used on both plates during inoculation, so both are treated identically at that stage; during the UV step, the lamp's glow falls only on the right plate's surface with none of it visible on the still-covered left plate; the right plate's lid is shown fully back in place before the labeling step begins; labeling happens after the lid is replaced, not while the plate is still open to the UV lamp.
level_5: Everything required for score 4 is shown AND: the left (control) plate's lid is never lifted at any point after the initial inoculation frame; the right plate's lid removal, UV exposure, and lid replacement happen in that order with no step skipped or reordered; both plates carry a legible label in the same style and position once labeling occurs; the spacing and orientation of the two plates on the bench match between the opening inoculation shot and the closing incubation shot.

Scientific and Causal Correctness rubric:
level_1: Neither plate shows any colonies after incubation, or both plates show a similar amount of growth, so there is no visible evidence that the UV exposure affected microbial survival; a UV lamp appears in the shot but nothing about the final plates shows it had any effect.
level_2: Growth appears on both plates in similar amounts; or the control plate shows colonies while the exposed plate instead shows non-biological damage (scorching, melted or dried-out agar) rather than a simple lack of colonies, leaving it unclear whether the UV light or some other damage caused the difference.
level_3: After incubation, the control plate shows clearly visible, discrete microbial colonies scattered across its surface, while the UV-exposed plate's surface is free of any colonies and otherwise looks like the same moist agar as the control, with no melting, drying, or discoloration; the difference is attributable to the earlier UV step and not to some other visible cause.
level_4: Everything in score 3 holds AND: the exposed plate has no colonies anywhere, including at its edges (no ring of surviving growth where the UV beam might not have reached); the control plate's colonies are numerous and look opaque and raised, consistent with live bacterial or fungal growth; nothing on the exposed plate (a scratch, a condensation droplet, a smudge) could be mistaken for a colony.
level_5: Everything in score 4 holds AND: the control plate's colonies vary naturally in size and spacing rather than forming a uniform, stamped-looking pattern; the exposed plate's agar surface is otherwise indistinguishable from the control's in color and moisture, with a total absence of any raised or opaque growth; a viewer can tell at a glance that the same starting inoculum produced abundant colonies where the UV light never reached it and none where it did.

Spatiotemporal Consistency rubric:
level_1: A plate that was on the bench in one shot is simply gone from the next shot with no hand, tray, or motion shown removing it; the camera cuts between a close view and a wide view of the plates with no camera move connecting them; the scene's brightness jumps from bright to dark, or the reverse, within a single beat with no dimmer or lamp switch shown causing it; a plate's lid bends or looks like it is melting; the UV lamp's glow switches on and off repeatedly with no hand near a switch; a plate's surface changes appearance (e.g., colonies appear) with no incubation time shown passing.
level_2: One of the plates shifts position on the bench between the inoculation shot and the UV-exposure shot with no pan or push-in to explain the change; the lid being lifted off the exposed plate skips straight from 'on' to 'off' with no lifting motion shown; the UV lamp's beam swings or wanders during exposure instead of holding steady on the plate; the shadow a lamp or hand casts changes shape between shots without the light or hand itself moving.
level_3: Both plates stay in the same spot on the bench for the whole sequence; no plate, lid, or lamp vanishes or jumps to a new position between shots; the swabbing motion, the lid removal, the lid replacement, and the labeling each play out as one continuous action rather than being spliced from separate takes; the UV lamp's glow comes on gradually as it is switched on, then holds steady while the plate is exposed.
level_4: Everything in score 3 holds AND: the lid-lifting and lid-replacing motions for the exposed plate are smooth and gravity-consistent (lifted straight up, set down without bouncing) rather than sliding or snapping into place; the UV lamp's brightness and violet-blue hue stay constant for the whole exposure instead of pulsing; the shadows the lids and plates cast keep the same direction and softness from the inoculation shot through to the final incubation shot; no action shows a jump cut partway through.
level_5: Everything in score 4 holds AND: the two plates keep the same spacing and relative size on screen from the opening inoculation shot to the closing incubation shot, with no drift between them while the camera holds still; every motion (swab stroke, lid lift, lid replacement) eases to a stop rather than halting abruptly; the UV lamp's glow neither flares nor dims at any point during exposure; no plate surface shows a stray reflection or flash that breaks continuity with the rest of the shot.