Package: pyBigWig
pyBigWigA python extension, written in C, for quick access to bigBed files and access to and creation of bigWig files. This extension useslibBigWigfor local and remote file access.Table of ContentsInstallationRequirementsUsageLoad the extensionOpen a bigWig or bigBed fileDetermining the file typeAccess the list of chromosomes and their lengthsPrint the headerCompute summary information on a rangeA note on statistics and zoom levelsRetrieve values for individual bases in a rangeRetrieve all intervals in a rangeRetrieving bigBed entriesAdd a header to a bigWig fileAdding entries to a bigWig fileClose a bigWig or bigBed fileNumpyRemote file accessEmpty filesA note on coordinatesGalaxyInstallationYou can install this extension directly from github with:pip install pyBigWigor with condaconda install pybigwig -c conda-forge -c biocondaRequirementsThe follow non-python requirements must be installed:libcurl (and thecurl-configconfig)zlibThe headers and libraries for these are required.UsageBasic usage is as follows:Load the extension>>> import pyBigWigOpen a bigWig or bigBed fileThis will work if your working directory is the pyBigWig source code directory.>>> bw = pyBigWig.open("test/test.bw")Note that if the file doesn't exist you'll see an error message andNonewill be returned. Be default, all files are opened for reading and not writing. You can alter this by passing a mode containingw:>>> bw = pyBigWig.open("test/output.bw", "w")Note that a file opened for writing can't be queried for its intervals or statistics, it canonlybe written to. If you open a file for writing then you will next need to add a header (see the section on this below).Local and remote bigBed read access is also supported:>>> bb = pyBigWig.open("https://www.encodeproject.org/files/ENCFF001JBR/@@download/ENCFF001JBR.bigBed")While you can specify a mode for bigBed files, it is ignored. The object returned bypyBigWig.open()is the same regardless of whether you're opening a bigWig or bigBed file.Determining the file typeSince bigWig and bigBed files can both be opened, it may be necessary to determine whether a givenbigWigFileobject points to a bigWig or bigBed file. To that end, one can use theisBigWig()andisBigBed()functions:>>> bw = pyBigWig.open("test/test.bw")
>>> bw.isBigWig()
True
>>> bw.isBigBed()
FalseAccess the list of chromosomes and their lengthsbigWigFileobjects contain a dictionary holding the chromosome lengths, which can be accessed with thechroms()accessor.>>> bw.chroms()
dict_proxy({'1': 195471971L, '10': 130694993L})You can also directly query a particular chromosome.>>> bw.chroms("1")
195471971LThe lengths are stored a the "long" integer type, which is why there's anLsuffix. If you specify a non-existant chromosome then nothing is output.>>> bw.chroms("c")
>>>Print the headerIt's sometimes useful to print a bigWig's header. This is presented here as a python dictionary containing: the version (typically4), the number of zoom levels (nLevels), the number of bases described (nBasesCovered), the minimum value (minVal), the maximum value (maxVal), the sum of all values (sumData), and the sum of all squared values (sumSquared). The last two of these are needed for determining the mean and standard deviation.>>> bw.header()
{'maxVal': 2L, 'sumData': 272L, 'minVal': 0L, 'version': 4L, 'sumSquared': 500L, 'nLevels': 1L, 'nBasesCovered': 154L}Note that this is also possible for bigBed files and the same dictionary keys will be present. Entries such asmaxVal,sumData,minVal, andsumSquaredare then largely not meaningful.Compute summary information on a rangebigWig files are used to store values associated with positions and ranges of them. Typically we want to quickly access the average value over a range, which is very simple:>>> bw.stats("1", 0, 3)
[0.2000000054637591]Suppose instead of the mean value, we instead wanted the maximum value:>>> bw.stats("1", 0, 3, type="max")
[0.30000001192092896]Other options are "min" (the minimum value), "coverage" (the fraction of bases covered), and "std" (the standard deviation of the values).It's often the case that we would instead like to compute values of some number of evenly spaced bins in a given interval, which is also simple:>>> bw.stats("1",99, 200, type="max", nBins=2)
[1.399999976158142, 1.5]nBinsdefaults to 1, just astypedefaults tomean.If the start and end positions are omitted then the entire chromosome is used:>>> bw.stats("1")
[1.3351851569281683]A note on statistics and zoom levelsA note to the lay reader: This section is rather technical and included only for the sake of completeness. The summary is that if your needs require exact mean/max/etc. summary values for an interval or intervals and that a small trade-off in speed is acceptable, that you should use theexact=Trueoption in thestats()function.By default, there are some unintuitive aspects to computing statistics on ranges in a bigWig file. The bigWig format was originally created in the context of genome browsers. There, computing exact summary statistics for a given interval is less important than quickly being able to compute an approximate statistic (after all, browsers need to be able to quickly display a number of contiguous intervals and support scrolling/zooming). Because of this, bigWig files contain not only interval-value associations, but alsosum of values/sum of squared values/minimum value/maximum value/number of bases coveredfor equally sized bins of various sizes. These different sizes are referred to as "zoom levels". The smallest zoom level has bins that are 16 times the mean interval size in the file and each subsequent zoom level has bins 4 times larger than the previous. This methodology is used in Kent's tools and, therefore, likely used in almost every currently existing bigWig file.When a bigWig file is queried for a summary statistic, the size of the interval is used to determine whether to use a zoom level and, if so, which one. The optimal zoom level is that which has the largest bins no more than half the width of the desired interval. If no such zoom level exists, the original intervals are instead used for the calculation.For the sake of consistency with other tools, pyBigWig adopts this same methodology. However, since this is (A) unintuitive and (B) undesirable in some applications, pyBigWig enables computation of exact summary statistics regardless of the interval size (i.e., it allows ignoring the zoom levels). This was originally proposedhereand an example is below:>>> import pyBigWig
>>> from numpy import mean
>>> bw = pyBigWig.open("http://hgdownload.cse.ucsc.edu/goldenPath/hg19/encodeDCC/wgEncodeMapability/wgEncodeCrgMapabilityAlign75mer.bigWig")
>>> bw.stats('chr1', 89294, 91629)
[0.20120902053804418]
>>> mean(bw.values('chr1', 89294, 91629))
0.22213841940688142
>>> bw.stats('chr1', 89294, 91629, exact=True)
[0.22213841940688142]Retrieve values for individual bases in a rangeWhile thestats()methodcanbe used to retrieve the original values for each base (e.g., by settingnBinsto the number of bases), it's preferable to instead use thevalues()accessor.>>> bw.values("1", 0, 3)
[0.10000000149011612, 0.20000000298023224, 0.30000001192092896]The list produced will always contain one value for every base in the range specified. If a particular base has no associated value in the bigWig file then the returned value will benan.>>> bw.values("1", 0, 4)
[0.10000000149011612, 0.20000000298023224, 0.30000001192092896, nan]Retrieve all intervals in a rangeSometimes it's convenient to retrieve all entries overlapping some range. This can be done with theintervals()function:>>> bw.intervals("1", 0, 3)
((0, 1, 0.10000000149011612), (1, 2, 0.20000000298023224), (2, 3, 0.30000001192092896))What's returned is a list of tuples containing: the start position, end end position, and the value. Thus, the example above has values of0.1,0.2, and0.3at positions0,1, and2, respectively.If the start and end position are omitted then all intervals on the chromosome specified are returned:>>> bw.intervals("1")
((0, 1, 0.10000000149011612), (1, 2, 0.20000000298023224), (2, 3, 0.30000001192092896), (100, 150, 1.399999976158142), (150, 151, 1.5))Retrieving bigBed entriesAs opposed to bigWig files, bigBed files hold entries, which are intervals with an associated string. You can access these entries using theentries()function:>>> bb = pyBigWig.open("https://www.encodeproject.org/files/ENCFF001JBR/@@download/ENCFF001JBR.bigBed")
>>> bb.entries('chr1', 10000000, 10020000)
[(10009333, 10009640, '61035\t130\t-\t0.026\t0.42\t404'), (10014007, 10014289, '61047\t136\t-\t0.029\t0.42\t404'), (10014373, 10024307, '61048\t630\t-\t5.420\t0.00\t2672399')]The output is a list of entry tuples. The tuple elements are thestartandendposition of each entry, followed by its associatedstring. The string is returned exactly as it's held in the bigBed file, so parsing it is left to you. To determine what the various fields are in these string, consult the SQL string:>>> bb.SQL()
table RnaElements
"BED6 + 3 scores for RNA Elements data"
    (
    string chrom;      "Reference sequence chromosome or scaffold"
    uint   chromStart; "Start position in chromosome"
    uint   chromEnd;   "End position in chromosome"
    string name;       "Name of item"
    uint   score;      "Normalized score from 0-1000"
    char[1] strand;    "+ or - or . for unknown"
    float level;       "Expression level such as RPKM or FPKM. Set to -1 for no data."
    float signif;      "Statistical significance such as IDR. Set to -1 for no data."
    uint score2;       "Additional measurement/count e.g. number of reads. Set to 0 for no data."
    )Note that the first three entries in the SQL string are not part of the string.If you only need to know where entries are and not their associated values, you can save memory by additionally specifyingwithString=Falseinentries():>>> bb.entries('chr1', 10000000, 10020000, withString=False)
[(10009333, 10009640), (10014007, 10014289), (10014373, 10024307)]Add a header to a bigWig fileIf you've opened a file for writing then you'll need to give it a header before you can add any entries. The header contains all of the chromosomes,in order, and their sizes. If your genome has two chromosomes, chr1 and chr2, of lengths 1 and 1.5 million bases, then the following would add an appropriate header:>>> bw.addHeader([("chr1", 1000000), ("chr2", 1500000)])bigWig headers are case-sensitive, sochr1andChr1are different. Likewise,1andchr1are not the same, so you can't mix Ensembl and UCSC chromosome names. After adding a header, you can then add entries.By default, up to 10 "zoom levels" are constructed for bigWig files. You can change this default number with themaxZoomsoptional argument. A common use of this is to create a bigWig file that simply holds intervals and no zoom levels:>>> bw.addHeader([("chr1", 1000000), ("chr2", 1500000)], maxZooms=0)If you setmaxTooms=0, please note that IGV and many other tools WILL NOT WORK as they assume that at least one zoom level will be present. You are advised to use the default unless you do not expect the bigWig files to be used by other packages.Adding entries to a bigWig fileAssuming you've opened a file for writing and added a header, you can then add entries. Note that the entriesmustbe added in order, as bigWig files always contain ordered intervals. There are three formats that bigWig files can use internally to store entries. The most commonly observed format is identical to abedGraphfile:chr1	0	100	0.0
chr1	100	120	1.0
chr1	125	126	200.0These entries would be added as follows:>>> bw.addEntries(["chr1", "chr1", "chr1"], [0, 100, 125], ends=[5, 120, 126], values=[0.0, 1.0, 200.0])Each entry occupies 12 bytes before compression.The second format uses a fixed span, but a variable step size between entries. These can be represented in awigglefile as:variableStep chrom=chr1 span=20
500	-2.0
600	150.0
635	25.0The above entries describe (1-based) positions 501-520, 601-620 and 636-655. These would be added as follows:>>> bw.addEntries("chr1", [500, 600, 635], values=[-2.0, 150.0, 25.0], span=20)Each entry of this type occupies 8 bytes before compression.The final format uses a fixed step and span for each entry, corresponding to the fixedStepwiggle format:fixedStep chrom=chr1 step=30 span=20
-5.0
-20.0
25.0The above entries describe (1-based) bases 901-920, 931-950 and 961-980 and would be added as follows:>>> bw.addEntries("chr1", 900, values=[-5.0, -20.0, 25.0], span=20, step=30)Each entry of this type occupies 4 bytes.Note that pyBigWig will try to prevent you from adding entries in an incorrect order. This, however, requires additional over-head. Should that not be acceptable, you can simply specifyvalidate=Falsewhen adding entries:>>> bw.addEntries(["chr1", "chr1", "chr1"], [100, 0, 125], ends=[120, 5, 126], values=[0.0, 1.0, 200.0], validate=False)You're obviously then responsible for ensuring that youdo notadd entries out of order. The resulting files would otherwise largley not be usable.Close a bigWig or bigBed fileA file can be closed with a simplebw.close(), as is commonly done with other file types. For files opened for writing, closing a file writes any buffered entries to disk, constructs and writes the file index, and constructs zoom levels. Consequently, this can take a bit of time.NumpyAs of version 0.3.0, pyBigWig supports input of coordinates using numpy integers and vectors in some functionsif numpy was installed prior to installing pyBigWig. To determine if pyBigWig was installed with numpy support by checking thenumpyaccessor:>>> import pyBigWig
>>> pyBigWig.numpy
1IfpyBigWig.numpyis1, then pyBigWig was compiled with numpy support. This means thataddEntries()can accept numpy coordinates:>>> import pyBigWig
>>> import numpy
>>> bw = pyBigWig.open("/tmp/delete.bw", "w")
>>> bw.addHeader([("1", 1000)], maxZooms=0)
>>> chroms = np.array(["1"] * 10)
>>> starts = np.array([0, 10, 20, 30, 40, 50, 60, 70, 80, 90], dtype=np.int64)
>>> ends = np.array([5, 15, 25, 35, 45, 55, 65, 75, 85, 95], dtype=np.int64)
>>> values0 = np.array(np.random.random_sample(10), dtype=np.float64)
>>> bw.addEntries(chroms, starts, ends=ends, values=values0)
>>> bw.close()Additionally,values()can directly output a numpy vector:>>> bw = bw.open("/tmp/delete.bw")
>>> bw.values('1', 0, 10, numpy=True)
[ 0.74336642  0.74336642  0.74336642  0.74336642  0.74336642         nan
     nan         nan         nan         nan]
>>> type(bw.values('1', 0, 10, numpy=True))
<type 'numpy.ndarray'>Remote file accessIf you do not have curl installed, pyBigWig will be installed without the ability to access remote files. You can determine if you will be able to access remote files withpyBigWig.remote. If that returns 1, then you can access remote files. If it returns 0 then you can't.Empty filesAs of version 0.3.5, pyBigWig is able to read and write bigWig files lacking entries. Please note that such files are generally not compatible with other programs, since there's no definition of how a bigWig file with no entries should look. For such a file, theintervals()accessor will returnNone, thestats()function will return a list ofNoneof the desired length, andvalues()will return[](an empty list). This should generally allow programs utilizing pyBigWig to continue without issue.For those wishing to mimic the functionality of pyBigWig/libBigWig in this regard, please note that it looks at the number of bases covered (as reported in the file header) to check for "empty" files.A note on coordinatesWiggle, bigWig, and bigBed files use 0-based half-open coordinates, which are also used by this extension. So to access the value for the first base onchr1, one would specify the starting position as0and the end position as1. Similarly, bases 100 to 115 would have a start of99and an end of115. This is simply for the sake of consistency with the underlying bigWig file and may change in the future.GalaxypyBigWig is also available as a package inGalaxy. You can find it in the toolshed and theIUCis currently hosting the XML definition of this ongithub.