Package: kfwy
KFWY Data Analysis ToolkitThis is a toolkit forWindowsthat automatically analyzes data for assays.Installing KFWYTools AvailableUsing the Data Analysis Tools (Tools 1-3)Using the Merge Tools (Tools 4-6)Understanding the OutputInstalling KFWYEnsure that Python 2.7 is installed (preferably the latest version).Open your Command prompt and run the commandpython --version.If the response isPython 2.7.XXthen you have the correct version of Python installed. Otherwise, you should download the latest Python 2.7 Windows installer, open the installer, and follow the installation instructions (Python 2.7.15 is the latest at this time)https://www.python.org/downloads/release/python-2715/.NOTE: If you think Python is installed but the above command does not work, try the commandC:\Python27\python.exe --version. If this command works but not the above command, then consider following these instructions to add python to your PATH environment variables.Open theAdvanced System Settings.ClickEnvironment Variables.SelectNew.Enterpythonas the Variable Name andC:\Python27as the Variable Value.Restart your Command prompt.If you choose not to do this, then you must replacepythonwithC:\Python27\python.exefrom here on out.Make sure that pip is installed. If you have the latest version of Python 2.7, then pip should already be installed.Run the commandpip --version.If the response ispip XX.X from ... (python 2.7)then you have the correct version of pip installed. Otherwise, you should follow the installation instructions athttps://ehmatthes.github.io/pcc/chapter_12/installing_pip.html#pip-on-os-x.NOTE: If you think pip is installed but the above command does not work, try the commandC:\Python27\Scripts\pip.exe --version. If this command works but not the above command, then consider following these instructions to add pip to your PATH environment variables.Open theAdvanced System Settings.ClickEnvironment Variables.Double click thePathvariable.SelectNew.EnterC:\Python27\Scripts.Restart your Command prompt.If you choose not to do this, then you must replacepipwithC:\Python27\Scripts\pip.exefrom here on out.Install kfwy from the Python Package Index with pip.Type the commandpip install --upgrade kfwy.You must have also some other Python modules installed.matplotlibpandasseabornxlrdopenpyxl
Use the commandpip install matplolib pandas seaborn xlrd openpyxl. This may take a few minutes to finish.Tools AvailableMembrane PotentialNADH RedoxH2O2Membrane Potential MergeNADH Redox MergeH2O2 MergeUsing the Data Analysis Tools (Tools 1-3)Place the raw data files (.txt) to be analyzed in a folder.Open the command lineNavigate to the foldere.g. if folder path is users/data_analysis, then type the commandcd users/data_analysisType commandpython -m kfwyto runUsing the Merge Tools (Tools 4-6)Place the excel files (.xlsx) to be merged in a folder.Open the command lineNavigate to the foldere.g. if folder path is users/data_analysis, then type the command "cd users/data_analysis" without quotationsType commandpython -m kfwyto runUnderstanding the OutputThis section explains in detail the significance of the results of the data analysis.Membrane PotentialMetadata Sheet- This sheet hosts information about the experiment itselfID - The experimental ID.Standard Curve - A true or false value indicating whether or not the standard curve calculation was used.Slope - The slope value entered by the researcher to be used in calculations.Y-Intercept - The y-intercept value entered by the researcher to be used in calculations.Substrates - A column containing the list of substrates used in the experiment.Additions - A column containing the list of additions used in the experiment.Date - The date the analysis was run in the form YYYY-MM-DD.Raw Data- The raw data taken from the lab tools.The data is in the form  X | Y | X |...Averaged Raw Data- The average membrane potential for 3-minute (i.e., 180 seconds) increments in each data set. Each data set is annotated with the buffer conditions of the assay at that time.Corrected Raw Data- The same data as the Average Raw Data sheet, but with an additional correction calculation which divides each average membrane potential by the the average membrane potential for Ala.Standard Curve Data- The results of the standard curve calculation on the raw data.The data is in the form  X | Y | X |...Averaged Standard Curve- The results of the standard curve calculation on the averaged data.NADH RedoxMetadata Sheet- This sheet hosts information about the experiment itself.ID - The experimental ID.Substrates - A column containing the list of substrates used in the experiment.Additions - A column containing the list of additions used in the experiment.Date - The date the analysis was run in the form YYYY-MM-DD.Raw Data- The raw data taken from the lab tools.The data is in the form  X | Y | X |...Stripped Data- Contains only the columns that correspond to the NADH data points.The data is in the form  X | Y | X |...Reduced Data- The same data as the Stripped Data sheet, but with an additional reduction calculation.y_new = (y_old-min)/(max-min)*100Averaged Reduced Data- The averages of the reduced data for 3-minute (i.e., 180 seconds) increments in each data set. Each data set is annotated with the buffer conditions of the assay at that time.H2O2Metadata Sheet- This sheet hosts information about the experiment itselfID - The experimental ID.Standard Curve - A true or false value indicating whether or not the standard curve calculation was used.Slope - The slope value entered by the researcher to be used in calculations.Y-Intercept - The y-intercept value entered by the researcher to be used in calculations.Substrates - A column containing the list of substrates used in the experiment.Additions - A column containing the list of additions used in the experiment.Mitochondria - The amount of mitochondria use (in milligrams).Date - The date the analysis was run in the form YYYY-MM-DD.Raw Data- The raw data taken from the lab tools.The data is in the form  X | Y | X |...Slopes Data- The slope for 3-minute (i.e., 180 seconds) increments in each data set. Each data set is annotated with the buffer conditions of the assay at that time.Corrected Slopes- The same data as the Slopes sheet, but with an additional correction calculation which divides each slope value by the mg of mitochondria used.Standard Curve- The results of the standard curve calculation on the Raw Data.Standard Curve Slopes- The results of the standard curve calculation on the Slopes Data.Corrected Standard Curve Slopes- The results of the correction calculation on the Standard Curve Slopes data.Membrane Potential MergeCombines data from all of the Averaged Standard Curve sheets in the Membrane Potential data analysis Excel files. Each row corresponds to a substrate. The Mean and SEM is calculated for each row.NADH Redox MergeCombines data from all of the Averaged Reduced Data sheets in the NADH Redox data analysis Excel files. Each row corresponds to a substrate. The Mean and SEM is calculated for each row.H2O2 MergeCombines data from all of the Corrected Standard Curve Slopes sheets in the H2O2 data analysis Excel files. Each row corresponds to a substrate. The Mean and SEM is calculated for each row.