Package: hAMRonization
hAMRonizationThis repo contains the hAMRonization module and CLI parser tools combine the outputs of
18 (as of 2022-09-25) disparate antimicrobial resistance gene detection tools into a single unified format.This is an implementation of thehAMRonization AMR detection specification schemewhich supports gene presence/absence resistance and mutational resistance (if supported by the underlying tool).This supports a variety of summary options including aninteractive summary.InstallationThis tool requires python>=3.7 andpandasand the latest release can be installed directly from pip, conda, docker, this repository, or from the galaxy toolshed:pip install hAMRonizationOrconda create --name hamronization --channel conda-forge --channel bioconda --channel defaults hamronizationOr to install using docker:docker pull finlaymaguire/hamronization:latestOr to install the latest development version:git clone https://github.com/pha4ge/hAMRonization
pip install hAMRonizationAlternatively, hAMRonization can also be installed and used ingalaxyvia thegalaxy toolshed.UsageNOTE: Only the output format used in the "last updated" version of the AMR prediction tool has been tested for accuracy. Older tool versions or updates which lead to a change in output format may not work.
In theory, this should only be a problem with major version changes but not all tools follow semantic versioning.
If you encounter any issues with newer tool versions then please create an issue in this repository.usage: hamronize <tool> <options>

Convert AMR gene detection tool output(s) to hAMRonization specification format

options:
  -h, --help            show this help message and exit
  -v, --version         show program's version number and exit

Tools with hAMRonizable reports:
  {abricate,amrfinderplus,amrplusplus,ariba,csstar,deeparg,fargene,groot,kmerresistance,resfams,resfinder,mykrobe,pointfinder,rgi,srax,srst2,staramr,tbprofiler,summarize}
    abricate            hAMRonize abricate's output report i.e., OUTPUT.tsv
    amrfinderplus       hAMRonize amrfinderplus's output report i.e., OUTPUT.tsv
    amrplusplus         hAMRonize amrplusplus's output report i.e., gene.tsv
    ariba               hAMRonize ariba's output report i.e., OUTDIR/OUTPUT.tsv
    csstar              hAMRonize csstar's output report i.e., OUTPUT.tsv
    deeparg             hAMRonize deeparg's output report i.e.,
                        OUTDIR/OUTPUT.mapping.ARG
    fargene             hAMRonize fargene's output report i.e., retrieved-
                        genes-*-hmmsearched.out
    groot               hAMRonize groot's output report i.e., OUTPUT.tsv (from `groot
                        report`)
    kmerresistance      hAMRonize kmerresistance's output report i.e., OUTPUT.res
    resfams             hAMRonize resfams's output report i.e., resfams.tblout
    resfinder           hAMRonize resfinder's output report i.e.,
                        ResFinder_results_tab.txt
    mykrobe             hAMRonize mykrobe's output report i.e., OUTPUT.json
    pointfinder         hAMRonize pointfinder's output report i.e.,
                        PointFinder_results.txt
    rgi                 hAMRonize rgi's output report i.e., OUTPUT.txt or
                        OUTPUT_bwtoutput.gene_mapping_data.txt
    srax                hAMRonize srax's output report i.e., sraX_detected_ARGs.tsv
    srst2               hAMRonize srst2's output report i.e., OUTPUT_srst2_report.tsv
    staramr             hAMRonize staramr's output report i.e., resfinder.tsv
    tbprofiler          hAMRonize tbprofiler's output report i.e., OUTPUT.results.json
    summarize           Provide a list of paths to the reports you wish to summarizeTo look at a specific tool e.g.abricate:>hamronize abricate -h 
usage: hamronize abricate <options>

Applies hAMRonization specification to output from abricate (OUTPUT.tsv)

positional arguments:
  report                Path to tool report

optional arguments:
  -h, --help            show this help message and exit
  --format FORMAT       Output format (tsv or json)
  --output OUTPUT       Output location
  --analysis_software_version ANALYSIS_SOFTWARE_VERSION
                        Input string containing the analysis_software_version for abricate
  --reference_database_version REFERENCE_DATABASE_VERSION
                        Input string containing the reference_database_version for abricateTherefore, hAMRonizing abricates output:hamronize abricate ../test/data/raw_outputs/abricate/report.tsv --reference_database_version 3.2.5 --analysis_software_version 1.0.0 --format jsonTo parse multiple reports from the same tool at once just give a list of reports as the argument,
and they will be concatenated appropriately (i.e. only one header for tsv)hamronize rgi --input_file_name rgi_report --analysis_software_version 6.0.0 --reference_database_version 3.2.5 test/data/raw_outputs/rgi/rgi.txt test/data/raw_outputs/rgibwt/Kp11_bwtoutput.gene_mapping_data.txtYou can summarize hAMRonized reports regardless of format using the 'summarize'
function:> hamronize summarize -h
usage: hamronize summarize <options> <list of reports>

Concatenate and summarize AMR detection reports

positional arguments:
  hamronized_reports    list of hAMRonized reports

optional arguments:
  -h, --help            show this help message and exit
  -t {tsv,json,interactive}, --summary_type {tsv,json,interactive}
                        Which summary report format to generate
  -o OUTPUT, --output OUTPUT
                        Output file path for summaryThis will take a list of report and create single sorted report in the
specified format just containing the unique entries across input reports.
This can handle mixed json and tsv hamronized report formats.hamronize summarize -o combined_report.tsv -t tsv abricate.json ariba.tsvTheinteractive summaryoption will produce an html file that can be opened within the browser for navigable data exploration (feature developed
with @alexmanuele).Using within scriptsAlternatively, hAMRonization can be used within scripts (the metadata must contain the mandatory metadata that is not included in that tool's output, this can be checked by looking at the CLI flags inhamronize <tool> --help):import hAMRonization
metadata = {"analysis_software_version": "1.0.1", "reference_database_version": "2019-Jul-28"}
parsed_report = hAMRonization.parse("abricate_report.tsv", metadata, "abricate")Theparsed_reportis then a generator that yields hAMRonized result objects from the parsed report:for result in parsed_report:
      print(result)Alternatively, you can use the.writeattribute to export all results left in the generator to a file (if a filepath isn't provided, this will write to stdout).parsed_report.write('hAMRonized_abricate_report.tsv')You can also output ajsonformatted hAMRonized report:parsed_report.write('all_hAMRonized_abricate_report.json', output_format='json')If you want to write multiple reports to one file, this.writemethod can acceptappend_mode=Trueto append rather than overwrite the output file and not include the header (in tsv format).parsed_report.write('all_hAMRonized_abricate_report.tsv', append_mode=True)Implemented ParsersCurrently implemented parsers and the last tool version for which they have been validated:abricate: last updated for v1.0.0amrfinderplus: last updated for v3.10.40amrplusplus: last updated for c6b097aariba: last updated for v2.14.6csstar: last updated for v2.1.0deeparg: last updated for v1.0.2fargene: last updated for v0.1groot: last updated for v1.1.2kmerresistance: late updated for v2.2.0mykrobe: last updated for v0.8.1pointfinder: last updated for v4.1.0resfams: last updated for hmmer v3.3.2resfinder: last updated for v4.1.0rgi(includes RGI-BWT) last updated for v5.2.0srax: last updated for v1.5srst2: last updated for v0.2.0staramr: last updated for v0.8.0tbprofilder: last updated for v3.0.8Implementation DetailshAMRonizedResult Data StructureThe hAMRonization specification is implemented in thehAMRonizedResult dataclass.This is a simple datastructure that uses positional and key-word args to distinguish mandatory from optional hAMRonization fields.
It also uses type-hinting to validate the supplied values are of the correct typeEach parser follows a similar strategy, using a common interface.
This has been designed to match thebiopythonSeqIOparsefunction>>> import hAMRonization
>>> filename = "abricate_report.tsv"
>>> metadata = {"analysis_software_version": "1.0.1", "reference_database_version": "2019-Jul-28"}
>>> for result in hAMRonization.parse(filename, metadata, "abricate"):
...    print(result)Where the final argument to thehAMRonization.parsecommand is whichever tool is being parsed.hAMRonizedResultIteratorAn abstract iterator is then implemented to ingest a given AMR tool's report
(via the appropriate subclassed implementation), hAMRonize results i.e. translate the
original inputs to the fields in the hAMRonization specification, and yield a stream of
hAMRonizedResult dataclasses.This iterator also implements a write function to enable outputting the contents
to a output stream or filehandle in either tsv or json format.Tool-specific IteratorsEach tool has a specific subclass of this abstract hAMRonizedResultIterator e.g.AbricateIO.AbricateIterator.These include an attribute containing the mapping of the tools original output report fields to the hAMRonized specification fields (self.field_mapping), as well as handling specifying any additional required metadata.Theparsemethod of these subclasses then implements the tool-specific parsing logic required.
This is typically a simplecsv.DictReaderbut can be more complex such as the json parsing ofresfinderoutput,
or the modification of output fields required to better fit some tools into the hAMRonization specification.ContributingWe welcome contributions for users in any form (from github issues flagging problems/requests) to pull requests of bug fixes or adding new parsers.Setting up a Development EnvironmentFirst fork this repository and set up a development environment (replacingYOURUSERNAMEwith your github username:git clone https://github.com/YOURUSERNAME/hAMRonization
conda create -n hAMRonization 
conda activate hAMRonization
cd hAMRonization
pip install pytest flake8
pip install -e .Testing and LintingOn every commit github actions automatically runs tests and linting to check
the code.
You can manually run these in your development environment as well.To run a full set of integration tests:pushd test
bash run_integration_test.sh
popdTo run unit tests that verify parsing validity for each tool
as well as generation of valid summaries you can use pytest:pip install pytest
pushd test
pytest
popdFinally to run linting and check whether your code matches the project
code style:pushd hAMRonization
flake8 . --count --select=E9,F63,F7,F82 --show-source --statistics
flake8 . --count --exit-zero --max-complexity=20 --max-line-length=127 --statistics
popdAdding a new parserIf you wish to add a parser for a new tool here are the main steps required:Add an entry into_RequiredToolMetadataand_FormatToIteratorinhAMRonziation/__init__.pywhich points to the appropriateToolNameIO.pycontaining the tool's Iterator subclassInToolNameIO.pyadd arequired_metadatalist containing any mandatory fields not implemented by the toolThen add a classToolNameIterator(hAMRonizedResultIterator)and implement the__init__methods with the approriate mapping (self.field_mapping), and metadata (self.metadata).To this class, add aparsemethod which reads an opened file stream into a dictionary per line/result (matching the keys ofself.field_mapping) and yields the output ofself.hAMRonizebeing applied to that dictionary.To add a CLI parser for the tool, create a python file in theparsersdirectory:from hAMRonization import Interfaces
if __name__ == '__main__': 
    Interfaces.cli_parser('toolname')Alternatively, thehAMRonized_parser.pycan be used as a common script interface to all implemented parsers.Finally, following the template intest/test_parsing_validity.py, please generate a unit test that ensures the parser is working as you intend it to!If you have any questions about any of this or need any help, please file an issue.FAQWhat's the difference between an Antimicrobial Resistance 'Result' and 'Report'?For the purposes of this project, a 'Report' is an output file (or collection of files) from an AMR analysis tool.
A 'Result' is a single entry in a report. For example, a single line in an abricate report file is a single Antimicrobial
Resistance 'Result'.Known IssuesHere are some known issues that we would welcome input on trying to solve!Limitations of specificationmandatory fields:gene_symbolandgene_nameare confusing and not usually both present (only consistently used in AFP). Means tools either need 1:2 mapping i.e. single output field maps to bothgene_symbolandgene_nameOR have fragile text splitting of single field that won't be robust to databases changes.  Current solution is 1:2 mapping e.g. staramrinconsistent nomenclature of terms being used in specification fields: target, query, subject, reference. Need to stick to one name for sequence with which the database is being searched, and one the hit that results from that search.sequence_identity: is sequence type specific %id amino acids != %id nucleotide but does this matter?coverage_depthseems to include both tool fields that are average depth of read and just plain overall read-count,contig_idisn't general enough when some tools this ID naturally corresponds to aread_name(deepARG), individual ORF (resfams), or protein sequence (AFP with protein input):change toquery_id_nameor similar?