Package: ebel
e(BE:L) is a Python package built for both validating and modeling information extracted from publications usingBiological Expression Language (BEL).
This software package serves a comprehensive tool for all of your BEL needs and serves to create enriched knowledge graphs
for developing and testing new theories and hypotheses.e(BE:L)have implemented several other knowledge bases to extend the BEL knowledge graph or map identifiers.BioGridChEBIClinicalTrials.govClinVarDisGeNETDrugBankEnsemblExpression AtlasGWAS CatalogHGNCIntActGuide to PHARMACOLOGYKEGGMirTarBaseDifferent resources from NCBIOffSidesPathway CommonsThe Human Protein AtlasReactomeSTRINGUniProtInstallationThe easiest way to install ebel is to usedocker-compose. See below instructions to use the docker installation.ebelcan be directly installed from PyPi with pip:$ pip install ebelBut we want to encourage you to use the latest development version which can be installed with:$ pip install git+https://github.com/e-bel/ebelPackage RequirementsInstalling OrientDBThis software package is designed to work in conjunction withOrientDB, a NoSQL, multi-model database
that acts as both a graph and relational database. e(BE:L) uses OrientDB for generating the knowledge graph derived from BEL files. To get
started with e(BE:L), firstdownload OrientDBand get a server up and running.
The first time the server is ran, you will need to create a root password. Once it is up and running, you can get
start importing BEL files into it!On Linux you can use following commands:wget https://repo1.maven.org/maven2/com/orientechnologies/orientdb-community/3.2.2/orientdb-community-3.2.2.tar.gz
tar -xvzf orientdb-community-3.2.2.tar.gz
cd orientdb-community-3.2.2/bin
./server.shSQL DatabasesThis package is capable of enriching the compiled knowledge graphs with a lot of external information, however, this requires
a SQL databases for storage. While, a SQLite database can be used, this is not recommended as the amount of data and
complexity of queries will be quite slow. Additionally, SQLite will not be directly supported, the methods will be built
such that they should work with both SQLite and MySQL, but we will not address performance issues due to using SQLite.Instead, we recommend setting up aMySQL serverorMariaDBto use with e(BE:L). By default,PyMySQLis installed as a driver by e(BE:L), but others can also be used.On Lunux Ubuntu you can use following command:sudo apt install mysql-server -yor:sudo apt install mariadb-server -yConfigurationBefore you start working with e(BE:L), a simple to use wizard helps you to setup all configurations. Make sure OrientDB
and MySQL (or MariaDB) are running. Then start the configuration wizard with:ebel settingsThe wizard will create the needed databases and users in OrientDB and MySQL/MariaDB.Package ComponentsTo test the different components you findhereseveral BEL and
already compiled JSON files.BEL ValidationBEL is a domain-specific language designed to capture biological relationships in a computer- and human-readable format.
The rules governing BEL statement generation can be quite complex and often mistakes are made during curation.
e(BE:L) includes a grammar and syntax checker that reads through given BEL files and validates whether each statement
satisfies the guidelines provided byBEL.bio. Should any BEL statement within the file
not adhere to the rules, a report file is created by e(BE:L) explaining the error and offering suggested fixes.You can use the following command to validate your BEL file:$ ebel validate /path/to/bel_file.belIn a single command, you can validate your BEL file as well as generate error reports if there are errors and if there
are none, produce an importable JSON file:$ ebel validate /path/to/bel_file.bel -r error_report.xlsx -jBEL documents should be properly formatted prior to validation. e(BE:L) contains a repair tool that will check the format
and it is highly recommended that this is used prior to validation. The repaired will overwrite the original if a new file
path is not specified. Here is an example:$ ebel repair /path/to/bel_file.bel -n /path/to/repaired_file.belImport ProcessBEL Modeling - OrientDBBEL files that have passed the validation process can be imported into the
database individually oren masse. During the import process, e(BE:L) automatically creates all of the relevant nodes and edges
as described in the BEL files. Additionally, e(BE:L) also automatically adds in missing nodes and edges that are known to exist
e.g. protein nodes with a respective RNA or gene node with have these automatically added to the graph with the appropriatetranslatedToandtranscribedToedges.Model Enrichment - MySQLe(BE:L) goes one step farther when compiling your BEL statements into a knowledge graph by supplementing your new graph model with information derived from several
publicly available repositories. Data is automatically downloaded from several useful sites includingUniProt,Ensembl, andIntActand added as generic tables in your newly built database.
Information from these popular repositories are then linked to the nodes and edges residing in your graph model, allowing for more complex and
useful queries to be made against your data. This data is automatically downloaded, parsed, and imported into a specified SQL database.Importing - Getting Startede(BE:L) supports OrientDB as graph database andMySQLandMariaDBasRDBMSMake sure you have downloaded/installed and runningOrientDBMySQL or MariaDBMySQLWindowsMacOSLinuxUbuntu, Debian, Linux Mint, …RedHat, Fedora, CentOS, OpenSUSE, Scientific Linux, …MariaDBWindowsMacOS (PKG,Homebrew)LinuxUbuntu, Debian, Linux Mint, …RedHat, Fedora, CentOS, OpenSUSE, Scientific Linux, …This can be configured as a service in both Windows and Unix systems.Set your MySQL connection parameters in e(BE:L):$ ebel set-mysql --host localhost --user root --password myPassWord --database ebelOnce you have made sure both OrientDB and MySQL are running, you can now import an e(BE:L) compiled JSON file:$ ebel import-json /path/to/checked_bel.json -u root -p orientdbPassword -d ebel -h localhost -p 2424After you have successfully connected to the OrientDB database at least once, the login credentials will be written to the config file and no longer need to be passed (same withenrichcommand):$ ebel import-json /path/to/checked_bel.jsonYou can also import all e(BE:L) compiled JSON files in a passed directory:$ ebel import-json /path/to/bel_json/dir/If you do no wish to enrich the graph, or wish to disable the protein/RNA/gene extension step, you can toggle these with the following options:$ ebel import-json /path/to/checked_bel.json -e -gYou can run an enrichment step later using theenrichcommand:$ ebel enrichThis command can also be given a list of resources to either skip or include during enrichment:$ ebel enrich -i uniprot,hgncor:$ ebel enrich -s intact,keggQuerying the GraphOnce the knowledge graph has been created (and optionally enriched), one can use e(BE:L) to query it directly.fromebelimportBelbel=Bel()relation_query="SELECT out.bel as subject, @class as relation, in.bel as object FROM bel_relation"df=bel.query(relation_query)# Returns results as pandas DataFrameresult_list=bel.query_get_dict(relation_query)# Returns results as a list of dictsTo specify a different graph database to connect to than the one in your config file, you can pass connection parameters when initializing theBelclass:fromebelimportBelconfig_params={"db":"my_db","user":"superdude","password":"password123","server":"localhost",# defaults to localhost if none given"port":"2424",# defaults to 2424 if none given}bel=Bel(graph_config=config_params)# To overwrite your default values in the config filebel=Bel(graph_config=config_params,overwrite_config=True)Docker installationMake suredockeranddocker-composeare installed.docker-compose up --build -d
docker exec -it ebel_ebel ebel settingsSeveral question will follow. You can accept the default values (just press RETURN) except the following questions:OrientDB server [localhost] ?
ebel_orientdb
OrientDB root password (to create database and users)
ebel
MySQL/MariaDB sever name [localhost]
ebel_mysql
MySQL root password (will be not stored) to create database and user
ebelIt’s strongly recommended, if you are using ebel in the production environment, to change the
standard root MySQL and OrientDB passwords in the docker-compose.yml file.To load example files in container and import.docker exec -it ebel_ebel git clone https://github.com/e-bel/example_json_bel_files.git
docker exec -it ebel_ebel ebel import-json example_json_bel_files/phago.bel.json -eTo enrich the network:docker exec -it ebel_ebel ebel enrichFollowing services are now available:OrientDB Studioe(BE:L) REST serverphpMyAdminAPIFinally, this package comes equipped with a built-in RESTful API using Flask. Users that have a running and populated set of databases
can also create a running API server which contains several queries for retrieving information from both the network itself, as well
as the downloaded enrichment information stored in the SQL database.This server can be activated using:$ ebel serveYou can also specify certain parameters as options:$ ebel serve -p 5000 --debug-modeDisclaimere(BE:L) is a scientific software that has been developed in an academic capacity, and thus comes with no warranty or
guarantee of maintenance, support, or back-up of data.